LOCUS       DASNNU010000001        94803 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.001, whole genome shotgun sequence.
ACCESSION   DASNNU010000001 DASNNU010000000
VERSION     DASNNU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 94803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.001"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            140..1198
                     /locus_tag="U8116_000001"
     CDS             140..1198
                     /locus_tag="U8116_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEN8433027.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQSMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1202..2236
                     /locus_tag="U8116_000002"
     CDS             1202..2236
                     /locus_tag="U8116_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEN8433028.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2338..3054)
                     /locus_tag="U8116_000003"
     CDS             complement(2338..3054)
                     /locus_tag="U8116_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN8433029.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(3023..3808)
                     /locus_tag="U8116_000004"
     CDS             complement(3023..3808)
                     /locus_tag="U8116_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEN8433030.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3799..4548)
                     /locus_tag="U8116_000005"
     CDS             complement(3799..4548)
                     /locus_tag="U8116_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN8433031.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4846..5370
                     /locus_tag="U8116_000006"
     CDS             4846..5370
                     /locus_tag="U8116_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEN8433032.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5386..6885
                     /locus_tag="U8116_000007"
     CDS             5386..6885
                     /locus_tag="U8116_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEN8433033.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6973..7575)
                     /locus_tag="U8116_000008"
     CDS             complement(6973..7575)
                     /locus_tag="U8116_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN8433034.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7679..8635
                     /locus_tag="U8116_000009"
     CDS             7679..8635
                     /locus_tag="U8116_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEN8433035.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8940..9896)
                     /locus_tag="U8116_000010"
     CDS             complement(8940..9896)
                     /locus_tag="U8116_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN8433036.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10070..10630
                     /locus_tag="U8116_000011"
     CDS             10070..10630
                     /locus_tag="U8116_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433037.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10623..11447
                     /locus_tag="U8116_000012"
     CDS             10623..11447
                     /locus_tag="U8116_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEN8433038.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11457..12074
                     /locus_tag="U8116_000013"
     CDS             11457..12074
                     /locus_tag="U8116_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN8433039.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12092..12775
                     /locus_tag="U8116_000014"
     CDS             12092..12775
                     /locus_tag="U8116_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN8433040.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13458..14741
                     /locus_tag="U8116_000015"
     CDS             13458..14741
                     /locus_tag="U8116_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN8433041.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14757..15644
                     /locus_tag="U8116_000016"
     CDS             14757..15644
                     /locus_tag="U8116_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN8433042.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15661..16497
                     /locus_tag="U8116_000017"
     CDS             15661..16497
                     /locus_tag="U8116_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN8433043.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16500..17123
                     /locus_tag="U8116_000018"
     CDS             16500..17123
                     /locus_tag="U8116_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433044.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17120..18412
                     /locus_tag="U8116_000019"
     CDS             17120..18412
                     /locus_tag="U8116_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN8433045.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18771..18868
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18984..19565
                     /locus_tag="U8116_000020"
     CDS             18984..19565
                     /locus_tag="U8116_000020"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEN8433046.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19568..20875
                     /locus_tag="U8116_000021"
     CDS             19568..20875
                     /locus_tag="U8116_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEN8433047.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21291..21779
                     /gene="purE"
                     /locus_tag="U8116_000022"
     CDS             21291..21779
                     /gene="purE"
                     /locus_tag="U8116_000022"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEN8433048.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21772..22896
                     /gene="purK"
                     /locus_tag="U8116_000023"
     CDS             21772..22896
                     /gene="purK"
                     /locus_tag="U8116_000023"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEN8433049.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22909..24204
                     /gene="purB"
                     /locus_tag="U8116_000024"
     CDS             22909..24204
                     /gene="purB"
                     /locus_tag="U8116_000024"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEN8433050.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24326..25714)
                     /gene="ltrA"
                     /locus_tag="U8116_000025"
     CDS             complement(24326..25714)
                     /gene="ltrA"
                     /locus_tag="U8116_000025"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN8433051.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(26283..26660)
                     /locus_tag="U8116_000026"
     CDS             complement(26283..26660)
                     /locus_tag="U8116_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433052.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            26916..27644
                     /locus_tag="U8116_000027"
     CDS             26916..27644
                     /locus_tag="U8116_000027"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEN8433053.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            27644..27898
                     /gene="purS"
                     /locus_tag="U8116_000028"
     CDS             27644..27898
                     /gene="purS"
                     /locus_tag="U8116_000028"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEN8433054.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            27900..28571
                     /gene="purQ"
                     /locus_tag="U8116_000029"
     CDS             27900..28571
                     /gene="purQ"
                     /locus_tag="U8116_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEN8433055.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            28572..30794
                     /gene="purL"
                     /locus_tag="U8116_000030"
     CDS             28572..30794
                     /gene="purL"
                     /locus_tag="U8116_000030"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEN8433056.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            30779..32218
                     /locus_tag="U8116_000031"
     CDS             30779..32218
                     /locus_tag="U8116_000031"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEN8433057.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            32241..33293
                     /locus_tag="U8116_000032"
     CDS             32241..33293
                     /locus_tag="U8116_000032"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEN8433058.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            33290..33868
                     /gene="purN"
                     /locus_tag="U8116_000033"
     CDS             33290..33868
                     /gene="purN"
                     /locus_tag="U8116_000033"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEN8433059.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            33865..35400
                     /gene="purH"
                     /locus_tag="U8116_000034"
     CDS             33865..35400
                     /gene="purH"
                     /locus_tag="U8116_000034"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEN8433060.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            35416..36690
                     /gene="purD"
                     /locus_tag="U8116_000035"
     CDS             35416..36690
                     /gene="purD"
                     /locus_tag="U8116_000035"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEN8433061.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            36812..37726
                     /locus_tag="U8116_000036"
     CDS             36812..37726
                     /locus_tag="U8116_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN8433062.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(37859..39424)
                     /locus_tag="U8116_000037"
     CDS             complement(37859..39424)
                     /locus_tag="U8116_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN8433063.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(39417..40868)
                     /locus_tag="U8116_000038"
     CDS             complement(39417..40868)
                     /locus_tag="U8116_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN8433064.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(40882..41898)
                     /locus_tag="U8116_000039"
     CDS             complement(40882..41898)
                     /locus_tag="U8116_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN8433065.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            42118..43206
                     /locus_tag="U8116_000040"
     CDS             42118..43206
                     /locus_tag="U8116_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEN8433066.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            43260..44786
                     /locus_tag="U8116_000041"
     CDS             43260..44786
                     /locus_tag="U8116_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEN8433067.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            44788..45993
                     /locus_tag="U8116_000042"
     CDS             44788..45993
                     /locus_tag="U8116_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN8433068.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            46250..47227
                     /gene="guaC"
                     /locus_tag="U8116_000043"
     CDS             46250..47227
                     /gene="guaC"
                     /locus_tag="U8116_000043"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEN8433069.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            47491..48915
                     /locus_tag="U8116_000044"
     CDS             47491..48915
                     /locus_tag="U8116_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN8433070.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            49376..50485
                     /gene="pdhA"
                     /locus_tag="U8116_000045"
     CDS             49376..50485
                     /gene="pdhA"
                     /locus_tag="U8116_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEN8433071.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            50488..51465
                     /locus_tag="U8116_000046"
     CDS             50488..51465
                     /locus_tag="U8116_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEN8433072.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            51490..53133
                     /locus_tag="U8116_000047"
     CDS             51490..53133
                     /locus_tag="U8116_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEN8433073.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            53141..54547
                     /gene="lpdA"
                     /locus_tag="U8116_000048"
     CDS             53141..54547
                     /gene="lpdA"
                     /locus_tag="U8116_000048"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEN8433074.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            54709..54984
                     /locus_tag="U8116_000049"
     CDS             54709..54984
                     /locus_tag="U8116_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEN8433075.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            54990..55757
                     /locus_tag="U8116_000050"
     CDS             54990..55757
                     /locus_tag="U8116_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEN8433076.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            55925..57754
                     /gene="typA"
                     /locus_tag="U8116_000051"
     CDS             55925..57754
                     /gene="typA"
                     /locus_tag="U8116_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEN8433077.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            58015..59787
                     /gene="recQ"
                     /locus_tag="U8116_000052"
     CDS             58015..59787
                     /gene="recQ"
                     /locus_tag="U8116_000052"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEN8433078.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            59901..60203
                     /locus_tag="U8116_000053"
     CDS             59901..60203
                     /locus_tag="U8116_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEN8433079.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            60206..61369
                     /locus_tag="U8116_000054"
     CDS             60206..61369
                     /locus_tag="U8116_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN8433080.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            61395..64823
                     /locus_tag="U8116_000055"
     CDS             61395..64823
                     /locus_tag="U8116_000055"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEN8433081.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            64844..65968
                     /locus_tag="U8116_000056"
     CDS             64844..65968
                     /locus_tag="U8116_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433082.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            65958..66395
                     /locus_tag="U8116_000057"
     CDS             65958..66395
                     /locus_tag="U8116_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN8433083.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            66379..66717
                     /locus_tag="U8116_000058"
     CDS             66379..66717
                     /locus_tag="U8116_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEN8433084.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            66836..67396
                     /gene="rsmD"
                     /locus_tag="U8116_000059"
     CDS             66836..67396
                     /gene="rsmD"
                     /locus_tag="U8116_000059"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEN8433085.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            67393..67884
                     /gene="coaD"
                     /locus_tag="U8116_000060"
     CDS             67393..67884
                     /gene="coaD"
                     /locus_tag="U8116_000060"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEN8433086.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            67874..68917
                     /locus_tag="U8116_000061"
     CDS             67874..68917
                     /locus_tag="U8116_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEN8433087.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            69076..69804
                     /locus_tag="U8116_000062"
     CDS             69076..69804
                     /locus_tag="U8116_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEN8433088.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            69920..70165
                     /locus_tag="U8116_000063"
     CDS             69920..70165
                     /locus_tag="U8116_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN8433089.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            70162..70380
                     /locus_tag="U8116_000064"
     CDS             70162..70380
                     /locus_tag="U8116_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEN8433090.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            70516..71055
                     /locus_tag="U8116_000065"
     CDS             70516..71055
                     /locus_tag="U8116_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEN8433091.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            71048..73321
                     /locus_tag="U8116_000066"
     CDS             71048..73321
                     /locus_tag="U8116_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEN8433092.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            73359..74405
                     /gene="holA"
                     /locus_tag="U8116_000067"
     CDS             73359..74405
                     /gene="holA"
                     /locus_tag="U8116_000067"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEN8433093.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(74494..74748)
                     /locus_tag="U8116_000068"
     CDS             complement(74494..74748)
                     /locus_tag="U8116_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEN8433094.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(75148..75417)
                     /locus_tag="U8116_000069"
     CDS             complement(75148..75417)
                     /locus_tag="U8116_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433095.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(75887..76342)
                     /locus_tag="U8116_000070"
     CDS             complement(75887..76342)
                     /locus_tag="U8116_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEN8433096.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(76480..78408)
                     /locus_tag="U8116_000071"
     CDS             complement(76480..78408)
                     /locus_tag="U8116_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN8433097.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(78605..79438)
                     /locus_tag="U8116_000072"
     CDS             complement(78605..79438)
                     /locus_tag="U8116_000072"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEN8433098.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(79630..80334)
                     /locus_tag="U8116_000073"
     CDS             complement(79630..80334)
                     /locus_tag="U8116_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN8433099.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(80357..80983)
                     /locus_tag="U8116_000074"
     CDS             complement(80357..80983)
                     /locus_tag="U8116_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN8433100.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            81309..83684
                     /locus_tag="U8116_000075"
     CDS             81309..83684
                     /locus_tag="U8116_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEN8433101.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(83708..84199)
                     /locus_tag="U8116_000076"
     CDS             complement(83708..84199)
                     /locus_tag="U8116_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEN8433102.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            84386..85306
                     /locus_tag="U8116_000077"
     CDS             84386..85306
                     /locus_tag="U8116_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN8433103.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            85460..85804
                     /locus_tag="U8116_000078"
     CDS             85460..85804
                     /locus_tag="U8116_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEN8433104.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(85994..86494)
                     /locus_tag="U8116_000079"
     CDS             complement(85994..86494)
                     /locus_tag="U8116_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8433105.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            87022..87105
                     /locus_tag="U8116_000080"
     tRNA            87022..87105
                     /locus_tag="U8116_000080"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:87056..87058,aa:Leu,seq:cag)
     gene            complement(87429..88067)
                     /locus_tag="U8116_000081"
     CDS             complement(87429..88067)
                     /locus_tag="U8116_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN8433106.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            88402..88836
                     /locus_tag="U8116_000082"
     CDS             88402..88836
                     /locus_tag="U8116_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN8433107.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            88859..89179
                     /locus_tag="U8116_000083"
     CDS             88859..89179
                     /locus_tag="U8116_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433108.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            89320..89511
                     /locus_tag="U8116_000084"
     CDS             89320..89511
                     /locus_tag="U8116_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433109.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(89582..89839)
                     /locus_tag="U8116_000085"
     CDS             complement(89582..89839)
                     /locus_tag="U8116_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEN8433110.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            89997..90260
                     /locus_tag="U8116_000086"
     CDS             89997..90260
                     /locus_tag="U8116_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433111.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            90284..90472
                     /locus_tag="U8116_000087"
     CDS             90284..90472
                     /locus_tag="U8116_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433112.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(90555..90740)
                     /locus_tag="U8116_000088"
     CDS             complement(90555..90740)
                     /locus_tag="U8116_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433113.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            90955..91326
                     /locus_tag="U8116_000089"
     CDS             90955..91326
                     /locus_tag="U8116_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN8433114.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(91476..91548)
                     /locus_tag="U8116_000090"
     tRNA            complement(91476..91548)
                     /locus_tag="U8116_000090"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91513..91515),aa:Gln,seq:ctg)
     gene            complement(91594..92022)
                     /locus_tag="U8116_000091"
     CDS             complement(91594..92022)
                     /locus_tag="U8116_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433115.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(92082..92840)
                     /locus_tag="U8116_000092"
     CDS             complement(92082..92840)
                     /locus_tag="U8116_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN8433116.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            93135..93332
                     /locus_tag="U8116_000093"
     CDS             93135..93332
                     /locus_tag="U8116_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433117.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            93438..93920
                     /locus_tag="U8116_000094"
     CDS             93438..93920
                     /locus_tag="U8116_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433118.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(93978..94574)
                     /locus_tag="U8116_000095"
     CDS             complement(93978..94574)
                     /locus_tag="U8116_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN8433119.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT        30644 a        16404 c        20156 g        27599 t
ORIGIN      
        1 cataagtttc aaaagtttct ttaatttcct attgttcaat aagtaacaat gtaaagataa
       61 aattaaaaat gttaatacgt attagcaaaa taaaagcttt ttttattgtg aataaatgaa
      121 cataattagg aggagaaaga tgaacttatt tgacattttt cgatcgactt tgacagatat
      181 gtcgattgtc agtgcaatca cttcaacagt ttttattata ttattagggt ttgtttgtag
      241 aaaaaagggg attttttctg aatctgttgg aaaaattcta tcgaaagtcg tcttaacagt
      301 ggctttgcca gcactggcat ttcgagcttt catgcaagat atcaatacag aattactgat
      361 gcaaggaatc aatgtcttga tttggggatt agttatttat attattctta ttctagtttc
      421 aaaacctttt ttcttaagct ataaagggga taagcaaaca acgttgcgtg tcctgacgat
      481 ctttggttct acgacctttt tcggaacgcc tatcgtcagt gcgatttatg gtcctgtcgg
      541 cgtgatgtat tcctcaattt tcaatattgg ctaccgaatc tttttgtatt cttatggata
      601 cataaaaatg agtggcttga agatggaagc taaaaatatc aaagcgatgt tcgtcaatcc
      661 aatcattatc gctacttttg ccggcctttt catttggctg ttccaagggt atctgccaca
      721 agtgtcagtt acaggtgcag aaggagagat aagccaagta gcgtttttgc gaattgacca
      781 gactgcaccg tggctatttc agtcgatgga ttatttagct ggtttaagtt caccattggc
      841 gtggctagca atcggcgcaa cattaggtga agtaagcttc aaaaaagctg catctgaaaa
      901 aacttcatgg tattacagtc tagtcaaagt ggtatttgta cctgcattga atatcgtgat
      961 tctagcagtt ttgacggcaa cgaatattct accagtcaat tttgaagcac tggcaacaat
     1021 cgttatcatg atggctacac cagctgcaac agtggcagca gcctatgcta tcagttttga
     1081 tagggaggca cttttagcct ccaatgcatc cttactgtca actatttttg ctgtggtact
     1141 gacacctgta tggatcgttg tattacaaat gattcatggc ataggtttat tttcataaga
     1201 aatggaggaa gaaagtatga gaatcgcatg ttacggtgtt cgtccgaacg agatagattt
     1261 ttttgaaaaa ttgaatattt accattatga tctcagtctt tatgaagaac tgttgacaca
     1321 tgacaatatc gtgtcagcaa aggaccatga tgctgtattg ttgaggggaa attgtatagc
     1381 agatgagacc aatcttgcaa aaatgcagga atatgggatt cgttatgttt ttacacggac
     1441 tgtgggagtc aatcatatcg atctacaagc ggcagctgat tttggtatga cagtagcccg
     1501 tgtaccatcg tattcgccaa atgcgatagc agagttatct ttgacttttg ccatgatgct
     1561 tttgagaaat actgcgtata cgacgatccg aacatctttc aaagattttc gagtggatga
     1621 gcaaatgttt agtcgtgaaa tcagaaattg cacagtaggg atcataggaa caggcaggat
     1681 agggttgacg gaagctgttt tgtttaaggg actaggagcc aaagtgatcg gctacgatat
     1741 tcatcagaca gaagctgcta aaaaagtatt gacgttccac tcgcttgaag agttattagc
     1801 caaaagcgat attgtcagta ttcatgtacc tcatatccca ggtgaaaacg atcaaatgat
     1861 caatgaagga tttttagctc aaatgaaaaa aggtagcatc ttgattaata cagcccgtgg
     1921 agagcttcaa gataatgaag ctattttaag agctttgaaa agcaatcatt tagctggttt
     1981 tgcaaccgat gtttttgcaa atgaaacaga ggtttttttc cgatccttca agccatggga
     2041 gacgattcca gaccctgcta tccagcaatt agtagaaatg taccctagag ttcttgtaac
     2101 cccgcatgta ggttcaaata cagatgaggc attatcaaat atgatttcga caagtttcga
     2161 gaactttcgt gaaataatag aaactggaaa aacgaaaaat gaggtatctc ttcctaaagc
     2221 ccgtcaatta agataagaaa acatggatat tcgtcaataa aatgtttaaa aatagtccaa
     2281 gtaaacagtt gttacttctg tatacttgga ctattttgta tgggataatt cgatgactca
     2341 acagtaatct gtgagctttt tccgcccatt ctttgtcaat tcataacagg ttagcggccg
     2401 gccgatttcc aaatatttga ttctttcgtt caatatatct tgttctacta aaaaattcaa
     2461 atatttcttg acggatatac gtgacagtga gcttttttga gccagttctt ttgttgaaaa
     2521 aatagtatct tcttttagaa taaatgcact tatttttttt aaagtcagtt ttgacagacc
     2581 tttaggcaaa tcgatgaatg atggtatttc gtgaatggga gtggtgtggt tgaatacttg
     2641 atcgatcaat ttctgatcgg ctttttcagt cgtttggatt gtctgagaga aagcaagata
     2701 cttttcgatc gccagctgaa aacgttgcaa agtgaacggt ttgatcaaat aatctaaggc
     2761 accgtagcgg atcacattct ttatggtttc gatatcatct gcagcagtga tcaaaatcac
     2821 tgggatatca tttccttgct gtctaagcca agataaaaaa tggataccac tttcgccagg
     2881 catgtataca tcaagcagca gcagatctgc gtttttttgt ctgaaatatt tttttccttc
     2941 ttctacattt ctgaaacttt cgattacgtt catattaggg atgctttcca caaaagtacg
     3001 attcaattct gcaaccattg gatcatcttc gattatcagg acatttgtca tcattttgat
     3061 cttccttttt tggtaattct agatagacga ttgtaccgcc atttgtttgt ttggaaaatt
     3121 ctaaaaatcc accgtggcgt ttgacgattg aatggatgag attcaatcca aacccacgat
     3181 tgattccctt cgtcgaaaaa cctagcgtaa atattttttc tgagatttct tctgatatcc
     3241 cgacaccgtc atcgcagacg gtcacgatga atacttcagc ttctgtgtca tagtttaaat
     3301 gaatccggac ctgtccgttt ctttcatgtt tttgtagcac ggcttctttt gcattatcaa
     3361 gtaagttccc taacacttgc aaaagtgaat ggatggagtc atccatctcc attttaggga
     3421 taaaagaatc gctttctaac agcatttcaa cagattgttc tttggcttcg tttattttgc
     3481 ctaataaaaa accggcaatt gcaggtactt tgatcagact tgagacttgg ccaatctctt
     3541 ttttataatt ttgatttagc tgctggataa aatgggtgac ttcttcatac ttcttcaaat
     3601 caatcaaacc agaaatgaca tgaagtttat tcatgaattc atgggtttga gctctcatgg
     3661 agtttatata ttgtttcgtc cccccaagct cctccattaa tttcattact tctgatcgat
     3721 ttcgtaaagt tgcgactgct cctaaaaaac ggtcattgct aaaaagaggt gtaacgctag
     3781 aaatagtttc tatcccattt aaatacatgg attgagtccg aacggtcgtt tgatgctcaa
     3841 tcgtctcttt aaacaaaacg tcatatagat tatcactgat ttttttccta cttcgaaatc
     3901 caatagctgc tctgtttggt agaaaatgtt ttttgcaggt gtattgatca agaggatagt
     3961 tttatcagaa gagatggcta agattccttc atctacttca ttttcgataa tcgttttttc
     4021 acttaactgt tctgctattt cttgtggttc aagtccaagc agtacttttt tcattttttg
     4081 ggcaagaaaa acggcgccta tgatgcctac gctaagtcca ataatcaatg aaaaaacaat
     4141 cattttttgt ccatctttga cttgattgtc aatcgtgttg tttgttaagc caacacaaac
     4201 tgcaccaata atctcttgat cataataaat agggacgaaa tagcggaagc cgaaccctaa
     4261 cattccttct ttttcagaga aaaattcttg tcctccaatc acttttttca catcttcatg
     4321 acttgagaaa ggaagaccga tgtaacttgg gtctgcgtga gaatatcgaa tgccttcagg
     4381 gtccatgaca acgataaaat ctaaattcga tgcaatcatg atttcatttg ttattccttg
     4441 aatcgcatgt gtcgggtcac cagtctgcag ctttttttgg atgagtttat cattggcaac
     4501 gattttagca atgctagaga ttttatctga cacactagat cggttcatcg aaagaataaa
     4561 attcctgatc aatatggcag aaacaatcaa agaaacgaat gtggagaaac aaacgacaag
     4621 gatgatcgtg ttctggagct ttaatggttt tttcttaaaa aaaggaaaca taggctacct
     4681 cgaaagaatt tattgtgaaa attttaacat gaaaacaaaa atttaacaaa ttgtcaacga
     4741 taaacacaac cttcctataa aaaacaccac tgagtcggta aaagaagcta tacagaattt
     4801 tcaaacattg gtatgataga cgtagaaatg gaaggaggcg tgtatatgat agggggaatc
     4861 gcacttgaga cgatttattg gtatacattg ctgatttgtg caggattggc agtcctattg
     4921 attatttttg gagatgtatt cgattttgat ggcccgcttg atccgatgct tattatccct
     4981 tggttaactt tcacaagctt atttggtttt ctaggtgagc gtttaacaga tcaatcatct
     5041 ttatttttat ttattgctag tggaggcata tcgatagtgt tggtattttt gcttaatttc
     5101 tatatattga tgccgatgaa acatgccgaa tcaacgatgt cgtcttctga aaaaactctg
     5161 gaaggacaag tagctgttgt gatcacaccg atccctgtac gaggtatggg agaaattcag
     5221 ttgaaaagtg ttaccggttc cattagtcgt ccagcttgtt tttacagccc ccaagaacag
     5281 acagctgagc ggggaacgaa agtattgatc attgagatca aggaaagaat ctgttatgtc
     5341 atgccttatg aaagcatgat aaaagcttaa aggagcgtgt gttaaatgga aataccagtt
     5401 gtttttatcg cgatcgcaat cgcagctttg attttactga tgttgctgat cgtctttgta
     5461 gcgaagtacc agacagcaaa accggatgaa gcgttgatca tcagtggaag ttatttagga
     5521 agcaaaaatg tgcacattga tgaaagcaat aacaaaatca aaattgtgcg tggaggtggc
     5581 gcattcgtct tgccagtttt tcaacgttcg aatcggatca gtctattatc gagtaaattg
     5641 gacgtctcca cccctgaagt gtatacggaa caaggcgtac cggtgatgtg tgatggtact
     5701 tcgatcatca aaatcggttc atctgtagaa gagattgcta cagcagcaga acagttttta
     5761 ggcaagacaa gagaagaact ggaaaatgaa gcaagagaag tattagaagg acacttgcgc
     5821 tcgattcttg gttctatgac tgtagaagaa atctatcaaa atcgcgataa gttcagtcaa
     5881 agcgtacaag aagtcgcttc tgtagacctt gcaaaaatgg ggttgatcat cgtttctttc
     5941 actatcaaag aggtcagaga caaaaacggt tatcttgact ctttagggaa accaagaatc
     6001 gcccaagtca aaagagatgc agatatagca gaagcggaag ctctaaaaga aacgcgtatc
     6061 aaaaaagctc aagcagaaaa agaatcacaa gcagctgaat tacagcgaca aacagagatc
     6121 gcagaatcat taaaagaaaa agaactgaaa ttagcaacct acaaacaaga acaagatgtt
     6181 gctaaagcaa aagcagacca agcctacaat ttagaaagtg cccgagcaca acaacaagta
     6241 atcgaacagg aaatgcagat taaaatcgta gaacgtcaaa aacaaatcga actggaagaa
     6301 aaagaaatca cacgtcgtga aaagcaatat gattcggaag tgaagaaaaa agcagatgcc
     6361 gatcggtacg ctaaagaaca agaagcacaa gctcaaaaag tcaaagaagt gactgaagcc
     6421 gaagcagaac gattccgtgt ggaagcactg gcagaagcgg aagccaataa gacacgttta
     6481 gctggacaag cagaagccga agcagcacta gcaaaaggaa aagcagaagc cgaagcaaaa
     6541 caaaagatcg ccaatgcctt caaggaatat ggcgaagcag ctgtcttgag tatggttatc
     6601 gatatgctac cgcaattgat gcgcgaagca gcacagccgc taggaaatat cgaaaaaatc
     6661 tctgttgtcg atacaggaag cagttcagga gaaaccagtg gagcgaaccg agtgacaaat
     6721 tatgccacaa atttgctttc ttctactcaa gaaacactaa aagcgacaac aggacttgac
     6781 ttgaaggaaa tcgtagagaa tttgtcgaca cgtgggacta ctagccaagt aaatatgtat
     6841 gaagcgccaa aagaggcaga acaaaactcg atgaaggaag aataaagtag tacacttcac
     6901 cgttcaatca aaaatggagg aagacttttt aaagttcttc tctccatttt tttattttat
     6961 tgcaaaagaa aattagtcct cgctattttt cttttgtttt tggatcttag cttctatctc
     7021 ttgtaaattt gttcttagca atcgttcttt cttttggagt tcgaccaaat cttctgatag
     7081 ttcttccttc ttttctgttt caggaggacg aagaaagaaa ttgataccga ttgcaatgaa
     7141 tgtccctata aaagtatcta gtacacgttg gatagcatac aaagcggatt cgccttgagg
     7201 gatgctcaac gcaatcagca acaacgtagc tattgcggaa atgataccgg aattattgtt
     7261 aataccatct gacacgacga tcacaataat aactagaata ggaagcaaga tcaattctac
     7321 caaaaaatca ttgtgaaaat agcttttgac aaaaaaataa atgatagcta atgtaccacc
     7381 taatgtattg ccgatgatcc tagactttcc aaaagaaacg gttgtagtca agtcctgacg
     7441 caaagaaaaa acagcagata atgcagcaat catcgggatt cctcgttgag tcaaatgaaa
     7501 aaacaagata cataaaaaaa cagctaaggc acttttgatc gtacgcatac ctagtcgaaa
     7561 acggccaaat tgcatagaga ttctcctttt tggtcttttt actatcatac ctaaactttt
     7621 gtctgatagc tagtttttca ttattcaagt ataattaaaa taaaaaaggg ggaagtaaat
     7681 gcgtatagga atgcgaacag tcaaaacagt gatcagtgtc ttcttttcga tggtcacagc
     7741 gggtagctta tcgctgttgt attggccagc tgcagggatc attgcattat taagtgtagg
     7801 aaataccaag cgctctacat tgcgtacggg catgaatcga atcgcagcct ttatattagc
     7861 tacggcaaca gcactgttca gtttttttgc tgttgggtat actatttttg gcttcagtct
     7921 ttttttattc ctgttcatcc cgcttgcctc tcgcttcaaa ttaaccgaag ggatagtagt
     7981 aaattcggtt ttggtcactc attatttagt ggaggaaaac atgtcttggc agctgatcgg
     8041 caatgaagcg ggattaatgg cgattggatt agtatttgct ttgcttgcaa atgtttacat
     8101 gccagatcgt acgaagcagc tgaaggaaaa ccaaatacag atcgaaaaag aatttcgaaa
     8161 tattttgaga caaatggcag aatttctttt atcagaaaat aaagaagatg tgcagatcaa
     8221 atgcgaacat ttactgacat tcattcgcga aagccaagaa gatgccagag aacatcaaga
     8281 aaactactgg ctcagacagc cgctatacta cgaaacttac ttctctatga gaagagcaca
     8341 ggccaatgtg attaaagaca tgctggaaaa tctagagaga atccagcaac cagcttatta
     8401 tggaaaacat atctatggtt tattgattta tactgcagaa acattttcag agtcaaatga
     8461 tggcagacaa attttaacac gtattgaaga agtttatgta ttgtatcggc aaatgcctct
     8521 gccgactagt cgccctgaat tcgaggatcg agcagaattg ttccagtttt tgcagtcttt
     8581 taaaagtttt attgaaatca aggcagaatt ctcccaacaa aaaatccaaa aatagaggtt
     8641 gtgaattcag ctaatattca ggtcaaaaag ctgatttcac aacctcttct ttaaacggtg
     8701 ttctcctgtc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgtttt
     8761 ttgaaattgc tccttgtgac tcggagctga acggtaggct cacaacctct ttcttaaacg
     8821 gtgttcaaaa gctttcctct gcggtaataa gtccagccaa acgaaaaata ggaggagcta
     8881 tttttgtttg gctgttctta ttacttgatg cagaacagct ttttcacaac cttttaaaat
     8941 tacatatttt tccatagaac ttgcatttga tccaagattt cgatgacatc ttttgtcaag
     9001 aagagagagc gattgggcaa gtcaccttca atcatgtgta ccatattctt tatttcgtaa
     9061 ttcattgctt cagatgtatt cccgctttcg atgaattcct ttgtaccatc tgtaaataag
     9121 atctccgcac gatcagcacg cgggtaatca gcaatcgtca cataaccgtt ctcaaaggca
     9181 acgataccga cttttggcat ttttgcctga aaagtcaagc tgacagcagc aagttcattt
     9241 tctttgttac gcaagatagt gacggattgt tcgtccacgc ctgtttcaaa aggaaccatt
     9301 gtagaggcaa ctacttcagg ttgagagctc aaaaagaaac gagcgaaaga tacagcatac
     9361 gtaccgatat ccaacagagc tcctccagct aattctggat tgaagaaccg gtttttaggg
     9421 tctggttctt tgtaactgcc gaaaggtgct tggatcattt ttaaagcccc cagttttcct
     9481 gtatccatta ttgagcgtaa ctgctgataa agtggcatgt tgaagattgt catcgcttct
     9541 gctaagatca aattcttttc ttctgctaag cgcatagcgt cagccagttc ttttttattc
     9601 atcgtgatgg ctttttcaca tagaacgtgt ttgcctgctt ccaatgctgc caagatatgg
     9661 tcgatatgct gtctatttgg tacagcaatg tacacgatgt cgatttcagc atcgtctaac
     9721 atttcttgat agcttccgta agcttttgga atattgtaac ggtgagcgaa gttttccgct
     9781 ttttctgaag ttctggaagc aactgctgtt agttcgctcg tctcttgatt gaagctttcg
     9841 gcaaactcat gtgcgatggt accaagacca acgattcccc attgatattt tttcatctga
     9901 aaacgctcct tcatagttgg attgagtttc ttgtctgtct attattataa caaaaatcac
     9961 gaagaataaa gaggagagga cttgaaacat taaaaaaaat gaaagcagcg atttaagagg
    10021 tgctttttga tcaaaactta agtagaatag ttaaaaggag gggataaacg tgcatcaagt
    10081 atatattgat cttattatga gtgtattatt agaacagttt gaaactgaaa aaaatttttt
    10141 agatgaatac ttgctgatca gtccagaaca atggcaaaaa tggaaagatg gcgagaaaaa
    10201 tctgccatct gaagcgatgc agaaagtaaa aaatcttttc actgattatg aatggatgct
    10261 aacgcaaaag attttgcgtc aaacgattat ttttcccgaa aaacgaaacg ttgccgttgc
    10321 agagtacaag caaatcaaaa cacgcatcgc gcaaaaatgg cttagcagca aagcagcaaa
    10381 agtcgagctg atccctttga aaaataatca gcaggctggt agataccttg acttgaaagt
    10441 aagtattcaa tatgatgaat ggggctatga tgatattttg aattttcgtc tgccagcaga
    10501 tgttcaaaaa cagatcgaag gagaaaaaat cgaacttctc gattgggtga atgaaaatct
    10561 ggaagaaact tacgttcatg aggcgaatgg ggaacgtgaa gaacaacgaa aggatttttc
    10621 aagtgaataa atttttagga tttggagcag ttgtgattat gatcgcagca atgggctatg
    10681 ttatttttag cgatgatgat tcgcatgttc aagccggcaa tcaagcagtc gaacgaacaa
    10741 gcaccaaaac aactgctagt aaagaaaaga acagtcaaac aaccagcagc aaggtaaagg
    10801 acaaagaaac aaaagatcgt acgtccaaac aattgccaga tgttaaaaca gacgattgga
    10861 agctagtttt ggttggtcca gatcacaaat tagaaaaaga aattgatgag tctactcaat
    10921 tggcatcatt aagtaacgga tacttgatcg acaaacgcat tgctccagaa tatgaagcat
    10981 ttgccaaggc tgctgaagca gctggatttc cattagtgat ggtctcagct taccggtctg
    11041 tatcatccca acaacaagtt ttcgaacaaa atgtccaaga tgtgatgagt cgtcaaggtg
    11101 tatcagaaga ggaggcgaca aagattacta aagaaacgat tacggagcca ggatacagcg
    11161 aacaccacac aggtctggca gtcgatgtcg tcgatcaaaa ctggtacaac agttatcctt
    11221 ctactgtatt ggatgcaagt tacggagatc agcctggagc taaatggatc gcagataatg
    11281 ctgcgcagta tggatttatc gttcgttatc caaaaggaca cgaagatata actaagatca
    11341 catatgaacc ttggcactta cgttatgtcg gtaaagaaaa cgctgagtat atcaccaaac
    11401 accaattgac gctagaagaa tatctgaatg aattaaatga aaagtaggaa ttgatcatgg
    11461 cgaaaaaaaa gtggaaaaag caaaagcgca agaatatccg ttggccagca gtggttgccg
    11521 gtgtagcagt gatactctta gcctttgttt tttccttgaa gaatctgtct tctcctttta
    11581 ctgataatca gcaagaaaat gaagcgcaat cccatcagca atttattgaa cggcttgttc
    11641 cgcatgccca agaactacaa gatggttatg gagtgcttcc tagtatcatt ttaggacaag
    11701 ctatattaga atcaaattgg ggaaaaagta ccttggctag caaatacaat aatcttttcg
    11761 ggatcaaggc ttatggggat cagaagaaag taagcctgga aacaaaggaa tttgttaacg
    11821 aagaatggat aactatccaa ggagacttca aagtgtatga ttcatgggaa cagtccatgg
    11881 atgaccatac acaactgttc gtccaaggag tagattggaa tcctgcgctg tatgaaaaag
    11941 tgattacggc caccaactat caggaagccg cacaagcatt gcaagatgca ggttatgcga
    12001 cagaccccgg ttatgcgcaa aaaatcatcc aggtcatcga gacgtatcaa ttgaatcagt
    12061 atgaccggag atagcaaagg agaagtggct gatggcagaa caatttacgc cagaaatcat
    12121 gacggaattg agaaaagata tcgtaagagt accggaaatc attttagaag caagcgggat
    12181 caagatattt ggaaaattga tccgttcgat catctttact acggatattg cgatcatacg
    12241 taatacaaat gcgaatgcag taattgctgt gtatcctttt acacctcatc cagcgattac
    12301 taaaagcatt attgaggcag ctgacattcc agttttctcc ggtgtaggtg gtggtttgac
    12361 acaaggtgct cgttcttcct acatgagcat gttcgcagaa gcgcaaggtt cgttaggggt
    12421 cgttttgaac ggtcctacgc ctttagagac agtaaaagaa gtttgtcgag tagttgatat
    12481 tccagttatc agtacggtaa cgtcgaaata tacagaaata gatgaaaagt tagcagctgg
    12541 tatttcgatt atcaatatca gtgcaggtaa agaaactgca gaagcagtac gccattttcg
    12601 taaaaaatat cctgaacttc ctatcattgc gacaggtggt ccaacggaag aaagtattgc
    12661 tgagaccatt caagcaggag caaatgccat cacatatacg ccaccaagta gcggagagct
    12721 ttttagtcgt aaaatggaaa aatatcggga aactgagaaa aagaatcatg aataacaggt
    12781 gagaatcaaa agacgaggaa gagcagatag tggctcttcc tcgtcttttg tactccagta
    12841 ctatttgttc agaattcaaa aaaaagagaa atttttttgt ttttttacct gtaatgtaca
    12901 taagaagagt taaaaccttt tgttattatt agcattctaa caaaaaaatg tcttgattta
    12961 ttgaagcgct ttctatagaa tgaaaaacga ataaaaacta aaaaaggcac ccgacaattt
    13021 agtagcagct aaattgtcgg ggcttgaaaa gtcagcggga actgaccaat caagttgcca
    13081 tagtcaatgc ttgtgcatcg acgtgtatat tgtacctaat ttcatacaga attactagtt
    13141 atttttctgt tttttaggga agagcgcaca cgaagaagca tgcttattta gctatgtcaa
    13201 ggatattggg cagttgtctg atatcctttt ttctttgcgt aattatcctc ccatcattga
    13261 agcaagaagt gtttttagcg atgaaagaag aatcagcact atctaatttt tcttgaaagt
    13321 attgagttgt tcatcctagg cagaacgata aggcaagtct tggtttttat tccccttgaa
    13381 aaagatcgtt taataaatat gttctgtata aaaatacagt atattgaaaa ataacaatgg
    13441 aagtagaggt gcagagaatg aagaaaagaa tgagaatcat cttagcttta agtgttatgt
    13501 tattcttctt gacggcttgt gggcaagaag atgacaaaaa aatcacgctg aacatggctt
    13561 ggtggggttc gcaagtccga cacgatgcaa cagtaaaagt aatcgagctt tatgaagaaa
    13621 aaaatcccca tgtgacgatc gattatgaat tttatgatta tgaaggatat tacacaaaat
    13681 taggtacact agcagcatca gatgatatct gggacatttt ccaactgaac aatcaatttc
    13741 ctcaatacat caatcagatt gaaccattag atgaatatgt ggagcaaggg ataattgata
    13801 tttctaatgc agatgaatcc tatttagcaa cgacgactta cgacgggcaa ttagttggac
    13861 tgtctaacgg ggtgaattcg tttgcgattg cttacaataa aaatatattt gaacaattaa
    13921 atatccctga accagcttca aattggacat gggatgaatt tgaacaaatc agctatcaaa
    13981 tcactgaaga attaggaatc tatgctattt caagattcga agattttata gctggctgta
    14041 ttattcaaat accacaagtt gagaaaggat tgaattttta taatcgggaa gatctatcta
    14101 acagcttggg atttgaaaat ccagactatc taacagattt tttccagctg cgtaaaaatc
    14161 tggtagacaa aggggcccat ccagaaccag gtggtggagc tgcaagtacg acggatctgt
    14221 cttttcaggc ggttagggat agagaagcgg caatggtgtt tctatcaagt aaccagttga
    14281 cagaaatatt gtcaggggct cctgaagata tggagattca gttgattaat ccacctcgca
    14341 gaaaagcaga tggagaatct ggtatccctt tacgttcttc gcaaatgctg agtatggcaa
    14401 aaagctcagc ccataaagaa gaagctgcta agtttattga tttctttcaa aacagtgaag
    14461 aagccaatga aattttaaaa ggagaacgtg gtgtaccaat catgtcgaat attcgagaac
    14521 agctcgctcg tgcagatgat gcaactttgc aagcgacatt tgattattta gaaatgattt
    14581 ccgatatgga caacggcgaa gtgaatgtct ttgatagtgc tttgaatccg gaaattcaag
    14641 accaatacaa tcttttagtt gaaaaagtca tttatgaaga agtaacagca gaagaagcag
    14701 cagagagtct ttatgagttt gcgtcaaaca ttctaggata gcagcaggag gaaggcatga
    14761 aaaaagaaaa actaaaacgg ttcttggatc aagacaatgt ggtcggttac gtatttgctt
    14821 ctccattcat cctaggattt ctattgataa cagtgatacc gatgatcttg tcactttatt
    14881 attcgttcac cgattatcgt ttgggaaatc cagtctcatg gatcggcttg gataattata
    14941 taaagatgct gagtgatgcc cgttttttga actccatccg agtcactttt ctctatgtat
    15001 ttgcatctgt acctgtgaaa ctgatctttg gtctattgat tgcttatctt ttaacgagga
    15061 atatcaaagg aacgactttc tttcgttctt tgtattatgt gcctagtttg atcggtggca
    15121 gtatttctat cgctctagtt tggcgggagt tgttttctaa aaacgggtta gtgaatattt
    15181 tcctggaacg attaggcgca cctgcgcttt cctggtttag cgatcaaaaa ctggtcatga
    15241 tccctcttgt tttgatgtct gcttggcaat tcggttcggc aatgatcatc tttgctgcag
    15301 gtatcaaaga gatcccaggc tcgtattatg aagcggcaga gatcgatgga gcgaacaaat
    15361 ggcagacgtt ttacaagatc acgttacctc ttctttcgcc gatcattttg tataacttgg
    15421 ttatgcagac aatcgcggcg tttatgactt ttacgcaggc atttatcatt acccaaggtg
    15481 gtccgaatga tgccacgaac ttttatgctc tttatgtgta taaccaagct ttttctttta
    15541 attctatggg gtatgctagt gctatttcgt ggatcatgct gttgattatg ggagctgtca
    15601 cctttctgat tttcaaatca tcaaaaaaat gggtcgttgt gtgaatgaga ggaggaagaa
    15661 atgaataata cagtaacgaa atggtttcaa tacggtttta ttttgctatt aggtctagtc
    15721 atgatttatc cggtcttatg gatgatcgca ggttctttta aaacggatca agagattatg
    15781 agtggttcgc tgaatttgat tccagagact tttcaatggg aaaattacgc aaaaggatgg
    15841 gcggggtttg ctggaatttc attttttact ttctttagga attcgtttgt catctctgca
    15901 gtttcgacga tagggacagt tctgagctcg acttgtattg cttacgcatt atcgagaatc
    15961 gatttcaaag gaaaaagatt ctggttcgtc gtcatgttag taacgatgat gattcctgca
    16021 caagtgattt tgattccgca gtttattatt tacaatcggc tgaatttggt tgggacgtat
    16081 gtgccgctga ttttaccaca tttttttgga caagcatttt tcatttacca gatcatgcaa
    16141 ttcatggtca gcatccccaa agagttagat gaatcagcga ttattgacgg atgcagcaag
    16201 tatagtgtct ttaccaaaat tatttttcct ttgctgaaac cttcgatcat cacgacgatc
    16261 atcatccagt tttattggaa atgggacgac ttcatgggac cattgattta tttaaacaag
    16321 ccgagatctt acacggtatc gattgccatc aaattatttg cagacgctgg atctagtacg
    16381 acttacgcga gtatgtttgc gatgtctacg ttgtcgctgc taccggtttt tctgattttc
    16441 ttattcttca ataaatatct tgtccagggg atcagtacaa gcggtctgaa gggctgaata
    16501 tgaaaaagcc aatagaagag atgtttcttt acaagctcaa ctactatgtc tgtcggattt
    16561 ttttaaccag tcttatcagt aatgctcttt tcgctttagt tctaggatgt ttgttcactt
    16621 taggattagt gctgtctgct cgtgttgtct atctgattta ttttttgatg atgtatcctt
    16681 cttattatct atctgtccat tacttggcag aaaaaaaggt atgttggagg aaaggaaaaa
    16741 tcaaacggat gtgtttcatc attacgttgt ttcagctgtt tgtcattatt tttgcagttg
    16801 attggtattt attagtggga aagattggag cttggtatct gattcctttt ctattgtgtc
    16861 tgttgctttt tgtatcccat agttttttta gtacgctaat tgatatctgc aaagaagata
    16921 aagcagatag tcttgtttat ttgatcaaaa ggaatatctg gctgttgtca aaaaacgtca
    16981 aggggtttct gtggtattta tgccttttgt atggactgtt ctggtgtata cagcatgatt
    17041 ttttactcac tgcattactg tatccagggc tgccatttgt cacagtattt tgcttaaaag
    17101 aaaaggagaa tgactttaaa tgaagaaaaa atatgtacaa gtcggacttg gtgggagagc
    17161 gcgttttttt tatgaggcaa ttgcctctac gtatcatcaa acttgtgaac tagtcggttt
    17221 ttgtgatgta tctcaaacaa ggatggatta tgcaaatcgg atacttgtga acaagtatgg
    17281 acagaaaaaa gtaaagacat gtatgccatc acaatttgag caactactgg atgacgtacg
    17341 gccggctgaa gtcattatta cgtcagtaga tcgaacgcat catgattata tcattcgtgc
    17401 aatgaaaaaa ggatatgatg ttatttgcga aaaacctctg actatcgatg aggaaaaagc
    17461 tcaagaaata ataacggtcc aaaaacagac gggaagaagc ctgcgagtca cttttaatta
    17521 tcgttatgca ccgtatcaca cgaagataaa ggaactgata gaaagtgatg tgattggtga
    17581 tgtgttttct atccacttcg aatggctgtt aaatacgcaa cacggggcag attatttcag
    17641 aagatggcat cgaaataagc acaatagcgg cgggcttctt gttcataaat ctacccatca
    17701 ttttgatcta gtgaattttt ggttgaacag ccgtccccaa aaggtatttg cattcggtgg
    17761 attaaacttt tatgggatgc ataatgcgga gaaacgcgga gtagaaatat tttacagtcg
    17821 atgctatgga agcgaactag ctaaagctga tccatttgct atagacctgt cagccaatga
    17881 ggaattgcga gaaatgtatt tagaagcaga aaaagacagt ggatatattc gtgaccgcag
    17941 tgtattttcc gatgatatct ctattgaaga tacactaggc gtaacggtca aatatgaaac
    18001 tggtgcgatt ttgacgtatt cgctaaacgc gtatatgccg tgggaaggat tcaatgtttc
    18061 tttcaatggc agcaaaggac ggatcgaaat gaaagtgatc gaaaagtctt acatcaatgc
    18121 aggtggagat aaaaaggatg aaggagcagc cgttcttcag tccatccatg tctatccgat
    18181 gttcggtgag ccatatgagg taatcgtcga acaagaagcg ggcggacatg gcggaggaga
    18241 tccgcgttta ttagatgatc tatttggtga agaaaaagaa gaagatccgt ggaatcgggc
    18301 agcaactcac gtagatggta ttttgtcgat tttgacagga atcgcagcaa atcattctat
    18361 tgcttcagga aaagcagtag tgattgacga gcttgtcagc ttaaaagaat aagaatagat
    18421 ttacggaaat tacttcccgt agtttataaa atcttgcctt attgccgata ggctgtcttt
    18481 tgaacactat ttattcggaa aaaggggcta tgacatgctt tttgtcatgc cttcttttta
    18541 tgtgggctgc ttttttagat aggagaaaat aaaaataact atttaaaccc gaacaaaaag
    18601 gaataatgca ttaaaaatca ttagatttta caaaaaaata agtaaaccac gttgtcagtt
    18661 tcagaaagta gtgttagact gttttcaata tttgaaacaa cattttttat tcaaatatgg
    18721 caaactgcct tcacgtgcaa aacagttgca agaaaatgaa tttacactat tattagtttt
    18781 acatacctac ttatatatcg tcataatatg gatgacagtt tctagccagt accgtaaatg
    18841 ctggactata agtaaaagat tggctatttc acccttcttt tcttatgata aagtggaggg
    18901 tgtttttctt taacagaaac atagtaggtt tttgcacaaa agcggttatt tcggtattaa
    18961 taaatgataa aggagcaaat aaagtgaaag aattagttga acgtattcaa aaagacggac
    19021 gtatattagg cgagggcgta ttaaaagtcg acagcttcgt cactcaccaa gtagaccctg
    19081 tattgatgga acaaatggga aaacggtttg cagaagtatt taaagaacaa ggaatcacga
    19141 aagttgtgac cattgaagct tctgggatcg caccagcttt atttgctgca agagaattag
    19201 atgttccaat gatcttcgct agaaaagcaa aaagtttgac gatggatgaa gaattattga
    19261 cagcttctgt ttattctttt acgaaacaag tgacaagtac gatttctatt tctcgtaaat
    19321 ttttatcttc agaagacaaa gtattgatta tcgacgattt cttggcaaac ggacaagcgg
    19381 caaaaggatt gatcgaatta tgccagcaag ctggagcgca agtagaagga atcgggatcg
    19441 tcatcgaaaa gtctttccaa gatggacgtc agttacttga agaaatggga ttgagagtcg
    19501 tttctcttgc gcgtattgct tccttgacga atggacaagt tgagtttctt gaggaggacg
    19561 cataatcgtg gaaaaagaaa cacaaaatgg aaaagctgcg gttttagggc tgcagcatct
    19621 gctagcgatg tatgcaggag cagtagcggt gcctcttttg atcggtacag gactaggctt
    19681 taatcaagaa cagatgactt atttgatttc aattgatatc tttatgtgtg gattagcgac
    19741 attattgcaa ttgacagtta accgattttt cggaattggc ttgcctgtcg ttttaggatg
    19801 tgcgatccaa gcagtagctc cactgatctt gatcggaacc aatgaaggag taggagctat
    19861 ctacggatct attatcgctt ctgggatctt tgttgtgtta gtttctggtg tcttttctaa
    19921 aattaaaaaa ctattccctc ctatagtaac aggaacggtt atcaccgtta ttggtttgac
    19981 tttgattcct gtagcaatcg agaaaatggg tggaggtaat gcaacagcag ctgattttgg
    20041 tgacaaaaca aatctgctac ttgcatttgt gacgattctc ctaattgtag gcgtgcaaat
    20101 gctggcaaaa gggttcattc gttcaatcgc ggtattgatt ggtttagtcg gaggaagcat
    20161 cttagctgct ttcatgggaa tgattgatct gagcgcgatt ggagatgcgc cgttgttcca
    20221 tgttccacaa cccttttatt ttggtaaacc aactttcgac gtatggtcga ttttactaat
    20281 gatcatcatt tccatggtca gcatggtaga atcaacaggt gtatattttg cacttggtga
    20341 cattactggg aaaaatgttg gagaagaaga attgaaaaag ggatatcgtg cagaaggact
    20401 tgccgttatc ttaggcggta ttttcaatac attcccttat actggctttt ctcaaaatgt
    20461 cggattggtt caactttcgg gaatcaaaac aagaaaaccc atctatttct ctgcattctt
    20521 cttgattgtt ttaggattat tccctaaaat cggtgcgtta gcacaaatca tccctgagcc
    20581 ggtacttggt ggaggaatgt tagtcatgtt cgggatggtg gcagtccaag gaatccgtat
    20641 gctgttggaa gttgatttca gcaatgataa aaatctattg attgttgctg tttctgttgg
    20701 atttgggctg ggtttcaatg ttacgccgac attattcagt caaatgccgc aaacattgca
    20761 aatgttcact ggaaatggca tcgtaatgag tagcattacg gctatcatat tgaatttgat
    20821 ctttaatgga ttgaaaaaag atgaagaaat attggaagaa gccttcaaga agtaatagag
    20881 caaaagaaag gttgtgaaat cagcgttttg acctgattgt tagagaaaca aacggaaaaa
    20941 tagttcctcg tattttgccg tttgtttgag ctaataccga gggtcagctg attgaacacc
    21001 gttgaaggaa gaggtcgtga gcctgccgtt catctccgag tcacaaggaa caatttcaaa
    21061 aaaaagcttc tcatgtttgt tgaatattgt tccttgtgac cgaggagttg gcaggagaac
    21121 accgtttatc cataaaaagg aatgtgacct aacttttgtc acattccttt tttttatctt
    21181 taataaacac gaacaataaa atgtttttct ttgttaacat tcgaaatttc tttgacatca
    21241 aaaaaagtga ttgttaaaat atgaatgtag aaaaactggg aggaaaaaca atgacagcaa
    21301 aaatctctgt gattatgggg agtacttcag attgggaaac aatgaaagaa gcatgtcaaa
    21361 tattggatga attcggtgtg tcttatgaaa aaaaggtcgt ttctgctcat cgaaccccag
    21421 atttgatgtt tgaatatgca caaaacgctc gaaaaaaagg aatcaaaatc attattgcag
    21481 gagcaggggg agccgcgcat ctgccgggta tggtcgcatc aaaaacaact ttgccggtta
    21541 tcggtgtacc ggtccaatca cgaacattaa atggtttgga ttcgcttctt tcgattgtcc
    21601 aaatgcctgg aggagtacct gtagctacta cagcaatcgg aaaagcggga gcaattaatg
    21661 ctggactact tgcgatacag atgctttcca tgtatgattt agaattggag gctaaattgg
    21721 ctgaaagacg ttcaatgttg gctaaaactg ttatagaaag tagtgatcaa cttggttaaa
    21781 cctttgttgc ctggttcaac gatcgggatc gtcggtggcg gtcaattagg tcgtatgatg
    21841 gcgattagtg caaaagaaat gggctttaaa gtaggtgtgc tggatcctgt gagcgattgt
    21901 ccagccgcac aagttgcaga ttggcacatt gaaagtgtct atgatgacac ctttgcgctg
    21961 aaagagcttg ccagaagaac agatgtcatt acctatgaat tcgaaaacgt cagtgtagag
    22021 gcgttaaatg cgattttacc catgtcattt attcctcaag ggacagactt gcttgcgatt
    22081 acgcaagacc gcctgttaga aaaatctttt ttggaaacga ataacatcgt gattgctcct
    22141 tatgcaacga tcgtcagtcc gacggatatc caagatgcga ttgacgggat cggctatcct
    22201 tgtgttttga aaaccactcg aggcggctat gatggcaagg ggcagtatgt tttgaaaagt
    22261 cgggcggatc tagctcctgc catggacctt ttaagagaag gaacctgtgt tttagaagca
    22321 tggatcccgt ttgaaaaaga gatttcgatc atggtggcag gaaatggtca aggagatttc
    22381 acgacttttc cggtcgtgga aaacatccac cacaacaata ttcttcatga aacgattgct
    22441 ccagccgcga ttgatcagga tgtgatcgag gaggctgagc gtattgcccg tgtgattgca
    22501 gaagcagtga gcctttcagg tgttttaggg gtagaaatgt ttttaacaaa aacaggcggg
    22561 ctatatgtga atgaattagc tcctcgtcct cacaattctg gacattattc tattgaagct
    22621 tgttctatga gtcagttcga tgcccatatt cgaggtattt gtggttggcc attaggagaa
    22681 aaagtacgtt tgataagcaa tgcagtaatg gtcaatgttt tgggagaaca actgctggaa
    22741 agctatcgct tgattgagaa taagccagac tggcaattcc atttctatgg aaaaaaacaa
    22801 gcaaaaaaag gtcgcaagat gggacatatc actatcttga cagaagacat ccagcaaacg
    22861 ctggaagaaa tcaaacaaac gaatatttgg gattagagga gaaaaaacat gttagaacgt
    22921 tatacacgcc ctgaaatggg gaatatctgg acggaagaga acaaataccg tgcgtggctc
    22981 gaagtcgaga ttttagcaga tgaagcttgg gcagaacttg gtgaaatccc gcaagaagat
    23041 gtgaaaaaaa ttcgtgagca tgcatcattt gatatcgatc ggatcttgga aatcgaaaaa
    23101 ttaacacgcc atgatgtcgt tgcgtttaca cgagcggttt ctgaaacatt gggagaagaa
    23161 agaaaatggg tccattatgg tctaacaagt acggatgtcg tagatactgc ttatggctat
    23221 ctcatcaagc aagcaaatga tattttacgg gaagacttga aacgttttac ggaaatcatt
    23281 ggcgaaaaag caaaagaaca caaatataca gtgatgatgg gtcggacaca cggtgtccat
    23341 gctgaaccaa ctacttttgg attgaaactt gccacatggt attcagaaat gaaacggaac
    23401 atcgaacgat ttgaacatgc ggcaaaagga gtagaagcag gaaaaatctc aggagcagtg
    23461 ggaacgttcg caaatatccc gccatttatt gaagagtatg tatgtaaaca attggggatc
    23521 cgtccacaag aaatctctac tcaagttctc cctcgggatc tacatgctga atatttctca
    23581 gcgatggcgc ttattgctac aagtattgaa cgttttgcaa cagagatacg cggattgcaa
    23641 aaatccgaaa cacgtgaagt agaagaattt tttgcgaaag gtcaaaaagg ttcttctgct
    23701 atgccgcaca aaaggaatcc gatcggttca gaaaatatgg ctggtctagc cagagtggtg
    23761 cgcggacaca tggtcactgc ctatgaaaat gtcacattat ggcatgaaag agatatttct
    23821 cattcttcag cagaacgaat catcttgcct gataccacga ttttattgaa ttacatgtta
    23881 aatcgttttg gcaatatcgt taaaaatcta acggtctttc ctgaaaacat gaagcggaat
    23941 atgtctgcaa catttggttt gatttatagc cagcgcgtta tgctgaaatt gatcgataag
    24001 ggaatgacaa gagaagaagc ttatgatctt gttcagccaa aaactgcgta cgcttgggac
    24061 aaccaagtgc agttccgccc gctgttagaa gaagatccaa ccatcacatc ggtattatct
    24121 aaagaagaaa tagacgatgc ctttgattat catcatcatt tgaaaaacgt tgacgtgata
    24181 ttcaaacgag taggtttgga ataaagaaga gtgagttgca aaattcaatt aaaatagaat
    24241 agcaaaaata atcgagtaga ggggaaagcc aactgacctt ccgcccctct cacaccaccg
    24301 tacgtacgga cccgtatacg gcggttcaat aagttgagtg tacgaactgg tacttcaagg
    24361 agatgtcctc atatccttgg cgggccagtt ctttgtctgt taatgatcga tgaagaatat
    24421 gacttttgga gactgaccaa ataccttttc ttgtatttga ccattcatac gctttcgctt
    24481 tatcgatccc taaacgaatc agattttttc ttttggttct tggattcttc cattgtttcc
    24541 aaatatattg tctgattctt cgcttcaacc aaccgtttaa ctccctcatg aattctttca
    24601 tctcaccaat tccatagtag ttgatccaac cagtcattag ttgcttgatt tcttttaaaa
    24661 taagttggat acttcttcca cggtttcttt tagtgagttg tttgagcttg cttttcacac
    24721 gctgttttgc ttttgcgtga gggcgaattt taataccttt ctttgttggc agaatgcaga
    24781 atccaagaaa ctttcgtttc aatggacttc ctaccgcact cttttctcga tttaccgtca
    24841 actttagttg ttcttcgaga aattgaatgg atttcttcat cacacgctgt cctgctcgtt
    24901 tgcttctaac atagatattg cagtcatcgg cgtatcgaac aaatttatgt cctagatttt
    24961 ctagcacttg atcaaattcg ttcaggtaga tgttacttaa caggggagag agattgcctc
    25021 cttggggcgt tccttcttca gttgttgtga tgagtccatt ttccattaca ccactcatta
    25081 agtattgacg gatcagtttt aggacttgtt tgtcctttac tttccgctca atgaaataca
    25141 tcagcttatc gtgatttacg gtgtcaaagt aggctttcat atcaatgtcc accacatatc
    25201 ggtaaccttg ttcataataa gattttgctt tcttcactgc gtcatgtgcg ctgcgatttg
    25261 ggcgaaaccc ataactatta tctgagaatt ctggttcaaa tagcggttgt aacacttgat
    25321 tgattgcctg ttgaagcata cgatcaataa ccgtgggaat tcctagtttt cttacaccgc
    25381 cgtttggttt cggtatttct actcgcaaaa caggctgagg tttatagttc ccttggcgta
    25441 aattggatag taattcctcg cggtgctctt tcaagtaagg aagcagttga tcgacggtca
    25501 tcccatctgc accagccgcc cctttatttc tgactacttg aagatacgct tgattgagat
    25561 tattccgatc tagaattctc ccaaacagat tttctacacc atcttgtttc ctctgttcac
    25621 cgcaagaagt gctgcgcact tcatcatacc cttcgtgttc cacactagct ctttgatgat
    25681 agccaagttt tcttgttttc tgcggtcgtc gcatcttctc acacctccta aattattcaa
    25741 gattcttatt gttcagtcct tcgtaacggt cagtgtcgtt actactatga cctcggctga
    25801 cttctcatga ttcaccatca tgtcgccatg atcgttgtgc tggtttgttt tcattcaacg
    25861 cccacaacac cctcatgaga tctcccccgg taagagtgaa aactttcctc ccatgtaact
    25921 gctagattta ctgtataggt ttcgggcagt attggacttt gtcttgtgat gaagacttat
    25981 ccgacctaat tcagccttgt atctagtttc tgttcgtcag ttcaggagtt tgcgtccgac
    26041 ttccttcagc cacaacctca cgattgccgc cttgtctttc gctaacagtt cctactgcca
    26101 agcctgtagt ggactttcac caccaagttt tcacccatgc agggcgcaca ccaaaaaaga
    26161 agcagatccg ttagcggttc tgcttctttt agtttattat attttcttat caataatagg
    26221 aacctgattt taaaagcggc tacctcttta ccgtgcctct ttacatacaa aaaatagagg
    26281 aattaagcgt aaatggcttg gctattataa ctaaaatgct ggttatggag atgttgcagc
    26341 aagttttcga gaaaagcgta ttccatctct tgtccattga ataagatctg gatacgataa
    26401 tgatttttga aacgcttctg ttcatgaatc tgactgattg aaagttcgcc tttgtgttct
    26461 gtctgataaa gaaaagtcct tttacggcat tcagcacagc gcttggttgc tcggcggatt
    26521 tttcggaatg tataaaaact agtatcttta ttgcataaat aaacgtcttt gatcgacagc
    26581 aaatcattat atagacagga acgttcttca gctgttaatt ctgataaaaa aagctgacct
    26641 tgttttattg catcttccat cggcaagtgc ctcctcgaac ccattatcca aatttttagt
    26701 taattctaat ataccatcat taagtataaa tgtaaacgct tgttgatggt ataagatttt
    26761 cgtttctgat aaaacacgaa cattaaaaga atctaatgcc ttaaatgtac atatttttgt
    26821 tgatttataa aaagatgatt gctaaaattt aaagtgaaaa aacgaacaaa aaacgatgta
    26881 tttgctttta atgtaaataa aaaggagaga cgtacatgga gaagaaagaa cttttgtatg
    26941 aaggaaaagc caaaaaaata tatcttactt ctgatgagaa cttgatttgg atagaatatt
    27001 tagatcaagc aactgcgttg aatggcgcga ggaaagacca gataagtgga aaaggagaat
    27061 tgaataatca aatcacaagt gcaattttcc gttatctgac tcaaagagga ataaaaaatc
    27121 attttattga acaattgtct aaaaccgaac aattagcaaa aaaagtacaa attatccctc
    27181 tggaagtggt tatccgaaat attgcagcag gaagtttctc aaaacgtcta ggtgttcccg
    27241 aaggtaaaga attcatcaaa ccaatcattg aattctacta taaggatgac cagttggatg
    27301 atccatttat caatgaagag catatctatt tcttacaact tgcaaagccg gaagaaatca
    27361 gtgagctgaa agcccaagcg ttggctatta atgaaatttt gaccgaatta tttgctaagg
    27421 tcggtttggt tttagtagat ttcaagctag aatttggccg gtcgaatgga caaattttgc
    27481 ttgcggatga agtaacaccg gatacttgcc gattatggga tgaacgtacg aatgaacata
    27541 tggacaaaga cgtttaccgc agagatctag gtgatttgat cccagtctat caagaagtat
    27601 ataaccgtct atcggaacta actgaagaaa aggagcgagt atgatgtatt tcgtaaaagt
    27661 atatgtaacc tataaagatt cagtgttaga tcctcaaggg gaggcagtaa aaaaagcagt
    27721 ccatcgtatg ggatatgaaa caatcgaaga tatccgaata ggaaaatact ttgagataca
    27781 acttgctgat gcagaagaac caatcgatac agtgattgaa acgatttgtg acaagctgct
    27841 tgcaaatgta aatatggaaa cataccgata tgaaatcaaa aaaatggagg aagtataaca
    27901 tgaaatttgc ggtcatcgtt tttccaggtt caaattgtga cgcagatatg ttatgggcag
    27961 ttcgcgagat catgggcgct gatgccgaat ttgtacggca tgatgagtcc tctttagaag
    28021 gatttgatgg ggttttactg ccgggaggat tctcctatgg tgactatttg agatgcggtg
    28081 cgattgctcg gttttctaca atcatggaag aagtgattcg ttttgctgaa gaagggaaac
    28141 cagtattcgg tacatgcaat ggattccaga tattgacaga agcaggactt ttgccaggtg
    28201 tactgagacg aaatgagtct ttgcatttta tttgcaaaac tgtaccattg aaagtcaacc
    28261 ccaagacggc ctttacttca gagtatgaag ccgacgaaat aatctatctt ccagttgctc
    28321 atggggaagg aaattatttc tgtgacgaag aaacacttca agaactaaaa aaaaataatc
    28381 agattgcctt tacttataat gacaacatca acggcagcgt ggaaaacatt gcagggatta
    28441 cgaacaaagc agggaatgtg ttaggaatga tgcctcatcc agaacgtgca gtagaagact
    28501 tgctaggatc agaagatggg aaacggttct ttgcttccat tttaaaaaac ttcggaaagg
    28561 tgaccgtata gatgaaaaca ttgaaagaac cgacagctga agagatcaag aaaaaaagaa
    28621 tctatgccga atggggattg actgatgaag aatatcgaat gatcgaagaa gatatcttag
    28681 gaagaatgcc taattatacg gaaactggac tgttttctgt tatgtggagc gaacattgtt
    28741 cctataaaaa ttctaaaccg gttctacgca aatttcctac tagtggacct catgtgttgc
    28801 aaggccctgg agaaggcgca ggcatcgtcg acatcggtga tggactagca gttgtcttca
    28861 aagcagaaag tcacaatcat ccttcagccg tggagcctta tgaaggagct gcgacaggag
    28921 ttggcggaat catcagagat attttcagca tgggggctcg tccaattgcg ttgctagatt
    28981 cattgaggtt cggcgaatta gataatccaa gaacgaaata tttactagaa gaagtagttg
    29041 caggtatcgg cggatacggc aattgtatcg gcataccgac tgtaggcggc gaaattgcat
    29101 ttgatccttg ttatgaagga aacccattag tcaatgcaat gtgcgtaggt ttgattgaac
    29161 acaaacatat ccagaaaggg caagccgctg gcgtcggtaa ttcgatcatg tatgtaggtg
    29221 caaaaacagg tcgcgacggt atccacggag caacttttgc ctcagaagaa tttaatcaag
    29281 aagaagaaca gcaacgttct gctgtacaag taggcgatcc ttttatggaa aagctgctgc
    29341 ttgaagcgtg tttggaatta atcttgcatc attcggattc tctaataggt atacaggata
    29401 tgggagcagc aggacttgtt tcatctagtg cggaaatggc ttcaaaagct ggctcaggac
    29461 ttatattgac attagatgaa gtcccacaaa gagaaactgg gatgacacct tatgaaatga
    29521 tgttatcgga atcacaagaa cggatgttga tttgtgttaa atcaggagag gaagaaaaaa
    29581 tccaagaact atttcagaag tatgatctag atgcagtcac tatcggtaaa gtaacagacg
    29641 acgggcaata tcggctatat catcaaggag aagaagtagc aaatctgcca gtcgatgcat
    29701 tagctgaaga tgcaccagtt tatcataaag aaatgaaaga accagctcga atcgtagaat
    29761 ttcagcagtt agctccttat cagccagtta tcgaagaacc tggacaagta ttgcttgatc
    29821 tgctgcagca accgacgatt gcttcaaaac gttcaatcta cgaaacttat gattcacaag
    29881 tacaaaccaa tacagttgtc cgtccaggaa gcgatgctgc tgtgatgagg gttagaggga
    29941 cgaataaagc gttagctatg accacagact gcaacgcgcg ctatctgtat ttggatccaa
    30001 aaatcggagg acaaatagca gtggcagaag ctgctagaaa tattattgct agcggtggta
    30061 agcccttggc gatcaccgac tgcctgaact atggttctcc tgataaacca gaagtttttt
    30121 gggaattatc gacttcagct gacggaattg ctgctgcgtg tgaaacgtta ggaactcctg
    30181 ttatttcagg aaatgtctct ttatataatg aaacggatgg tcaagcaatc tatccaacac
    30241 caatgattgg tatggtcgga ttgattgagg atcataaaca tatcactact caggaattca
    30301 aaaaatcagg ggatctaatc tatatattag gtaagacgtt tgcagatttt gatggcagtg
    30361 aattacaaaa gatgcagctt ggaagaatcg aaggtgtgat ccgaaacttt gatctatcca
    30421 ttgaaaagag aaaccaagag ttagtattga cagccatcca aaacggattg attgaaagtg
    30481 ctcatgactg ttcagaaggt ggcttggcaa ttgcactggc agaatcagct ttcaaacatc
    30541 agctagggat atctgtacag tttgaattgt cctctgctca attatttgca gaaactcaat
    30601 caagatttgt gttgacagtc gcaccggaaa ataagacacg attcgaagaa atgatgggag
    30661 atgctgctgt tttagcaggt aaggtgacgg atgaagcgat aatcgaaatt tcagcaactg
    30721 acggtcagat caaaatagaa actgcagtag caagaaagtg ctgggaggat gcaatcgtat
    30781 gtcttatgaa gtgaagagtt taaatgaaga atgcgggatc ttcggtgttt ggggtcatcc
    30841 agacgccgct agtgtggcat acttcgggct ccatagtctt cagcatcgag gacaagaagg
    30901 tgcaggtatc gtgtccaatg atcgaggaaa gcttaagggg catcgagatt tggggctgct
    30961 ttctgaggta ttcaaagatc aaagaaaatt gcagcagtta acaggagaag ctgcaatcgg
    31021 acatgtacgt tatgcaacag caggcaatgg aagtgtggac aatattcagc cttttctatt
    31081 caaatttcat gatcagtcga tcggcttagc gcataatggt aacttgatca atgctcgttc
    31141 ccttagaaaa gagttagagg aaaatggggc gatcttccat tctaactccg atacggagat
    31201 attgatgcat ttgatccgac gaagtgaaga aacagatttt ttaaataaat taaaaagtgc
    31261 tttgaatgaa gtcaaaggtg gctttgccta tttattaatg acagaaacgg caatgattgc
    31321 cgctttggac ccaaatggtt ttcgtccatt agcaatcgga caaatgaaaa acggagcata
    31381 cgtgattgcc tcggaaactt gtgcattaga agttattggg gcacgtttta taagagatgt
    31441 ccagcctggt gagatagtga tcatcaatga cgaaggaatc cagattgatt gctttacgaa
    31501 ggacacgcag ctttctattt gttcgatgga atatatctat tttgccagac cagattcaaa
    31561 catcgcaggg atcaatgtgc atactgcacg gaaaaatatg ggacggaatt tagcgaaaga
    31621 atcgccagta aaagcagata tggtcgttgg cgtacctaat tcttctttgt cagccgcaag
    31681 cggttatgcg gaagctagtg gaatccctta tgaaatcgga ttagtaaaaa atcaatacat
    31741 tgcccgaaca tttatccagc caacgcctga gctaagagag caaggtgttc gaatgaaatt
    31801 atctgctgta cgaggagtag tggaaggtaa aaaggtgatt atggtcgatg attcaattgt
    31861 tcggggtacg acaagcagac ggatcgtcca tcttttgaaa gaagcagggg caaaagaagt
    31921 ccatgtgagg attgcttctc ctcctttgaa atatccatgt ttttacggga ttgatattca
    31981 aacgcgtaaa gaactcattg cggcaaatca cacgattgat gaaatcaaag aatgtatcgg
    32041 tgcagattct ttaggttttt taagtgaaga gggtctgatc gaatcgatcg gtctggaaaa
    32101 agatgctccg tattcaggat tgtgtatggc ttatttcaac ggagattatc cgacgccgtt
    32161 atatgactat gaagaaaaat atcaggaatc actaaaagaa aaagtatcgt tcttttaaaa
    32221 gcagaccaag aagggagtat atgaattcaa tggcaaatgc atacgcaaaa gctggcgtag
    32281 atgtcgaagc tggatatgaa gtagtagaac ggatcaagaa gcatgtcaaa aaaacggaac
    32341 gtttaggtat gatgggcgct ataggaggat tcggcggttg ttttgattta tcggtctttc
    32401 aattaaaaga gccagtcctt gtctcgggga ccgatggcgt ggggacgaaa ttagcagtgg
    32461 caatcgaagc agacaaacac gatacaatcg ggatcgactg cgtagcgatg tgtgtcaatg
    32521 atatcttagc acaaggcgca gagccgttat atttcttaga ctacttagca acggggaaaa
    32581 atgatccgtt acgtttggaa aatgtagtaa aaggtgtagc agatggatgt gttcaaggaa
    32641 atatggcgct gattggcgga gaaacagctg aaatgcctgg tatgtactca ggtaatgatt
    32701 atgatctagc cggatttgct gtagggatag ctgaaaaatc tcaattattg acaggagaaa
    32761 cgatcaaccc aggagatgtg ttgcttggaa tagcatctag cgggattcat tcaaatgggt
    32821 actctcttgt tcgcaaaatt ttctttgagc agcagcagtt ttctttatca tcaaaactgc
    32881 cagaattgaa aaaaacactt ggcgaagaat tgcttgagcc aacgaaaatc tatgtttctt
    32941 ccttgcttcc gctgatcaaa gaaaagaaaa taaaaggagc tgcacatatc actggtggag
    33001 ggtttgtaga aaatatcccg cgaatgttac cagaatctat ggctgcacaa atcaaacttg
    33061 gctcatggcc gattcttcca atattcggct gtttacaaaa atatggtgaa attcctgaga
    33121 tggagatgta tgaaatcttc aatatgggca tcggtatggt cctggcagtc caagaatcga
    33181 aagtaaaaga aatagtacac gaaattgagc aagctggcga aaaagtttat gaaatcggga
    33241 aagtgatcgc caaagaaaaa catcgtgtgg tgtttatagg agaagaacga tgagaatcgc
    33301 tgtttttgct tctggaaacg gaagcaactt tcaagcgcta gccgattatc tatcgaaaaa
    33361 aggattggaa tcttcgattg actggttgtt ttgtgatcag cctgaagcat atgttttgaa
    33421 acgtgcaact gcgttgagtg taccggcaga ttgtttttca cctaaagaat ttgattcaaa
    33481 aaaagagtat gaagaagcaa ttttgcataa attgaaagaa aaaaagatcg atttgatcgt
    33541 attagctgga tatatgcgaa tcattggacc ggtattgctg aaaaattatg ataaaagaat
    33601 catcaatatc catccgtctc ttttacctgc cttccctggt ttgcatggca tacgtgatgc
    33661 ctttgaagct ggagtaaaag aaacgggtgt cacaattcat tatatcgacc aaggtgtaga
    33721 tactggacca atcatccggc aggaaaaagt gagaattgaa caggaggaca cgttcgattc
    33781 tttagaagaa aaaatccata gagtagagca tcgaatctat ccagaagtga ttagtgaaat
    33841 tattgaaaat ggaggctatc tgaaatgaca agagcattga tcagtgtatc agataaaact
    33901 gggattgtag cattcgccca aggtttgatt gaacaaggag tagaaatcat ttcaacagga
    33961 ggaacaaagc atgcgttgga taaagctggg atcaaaacgc tttcgatcga agaagtgaca
    34021 gggtttcctg aaatgatgga tggccgtgtc aaaacacttc atcctaaaat ccatggtggt
    34081 cttttaggaa gacgcgatct ccctagccat atggaggcaa tggaaacgga aaatatccaa
    34141 ccaatcgact ttgtttgtgt caatttatat cctttcaaag aaacgattat gaaaccagat
    34201 gtcgaaatag cagaagctat tgaaaacatt gatatcggtg gtcctagtat gctccgtagt
    34261 gcagcaaaaa accatgaatt tgtcacctct gttgttgatc catctgacta tgaagaagta
    34321 ctttcagaat tacgtcagta tgggcatact tcactttctt tcagacaaaa actagcggca
    34381 aaagtctttc gtcatactgc ggcatatgat gcactgatcg ctcaatactt aacggattgg
    34441 gtggaagaaa aagaaccgga aaaattgaca cttacgtatg aacgaaaaca aacgcttcgt
    34501 tacggtgaaa atagccatca agccgctgcc ttttatcagt ccgctcttct ggaaaaatat
    34561 tcgatcgcag cagcaaaaca actccatggg aaagaacttt cttataataa tatccgagat
    34621 gcagatgctg cgctacggat catgaaggaa tatgacgagc caacggtagt cgccttgaaa
    34681 cacatgaatc cttgtggtat tgggacagca tctacgattt tagaagcgtg ggaagcagcg
    34741 tatgaagcag atcctgtttc aatctttggt gggatcatcg ttttgaatcg agaagtagat
    34801 ctgccaacag cagaacagat gcataagctg tttctagaaa tcatcattgc cccttcttat
    34861 acacaagaag ctttggaatt actgcaaaaa aagaaaaaca tccgattact gacagtagac
    34921 tttgcgcagg aagaaaatca aaatcgagaa gaaacggtct cagtcttagg tggacttctt
    34981 gtacaagatc aagatctggc aatagaaaat gaagaagagt ggaaagtcgt gacaaaacgt
    35041 aagccgactc cagcagaagc aaaagcatta gcgtttgctt ggagagctgt caaacatgtg
    35101 aaatctaatg cgatcgtact ggcaaacgaa cgtcagacag taggaatcgg agccgggcag
    35161 atgaatcgag tcggttcagt aaaaattgcg attgaacaag cgcaagagaa aatggaaggt
    35221 gcagttttag ctagcgatgc ttattttcca atggatgatt cggttgaata tgcagccaaa
    35281 catggtataa aagcaatcat tcagccagga ggaagcatca aagaccaaga ttctattgat
    35341 atggcgaata aatacgatct agcaatggtc tttactgatg ttcggcattt tagacattag
    35401 agaggagtcg gataaatgaa cattttggtg atcggtgcag gtggtcgtga acatgcaata
    35461 agtaaaaaac ttttagccag tccgaaggtg gatacggtgt attgtgcgcc aggaaatccg
    35521 gggatgacaa aagatggtat caattgtgtc gctatcagtg agcgagatca ttcagcgtta
    35581 atccattttg ttaagaaaga ggctattgat tggagtatcg ttggtcctga agttcctttg
    35641 ttaaatggga tcgtcgatga ttttatggcg gaaggattga aaatcttcgg tccgacaaaa
    35701 gcagcagctc tgatcgaagg ttcgaaaagt tttgctaaac aattaatgga tacttatcaa
    35761 atcccgacag ctaaatccag aagttttgaa tgttacgaaa aagcagcagc gtatatcaaa
    35821 gaaaatggtg cgccaatcgt agtgaaagcg gacggcttag cagctgggaa aggtgtagtc
    35881 gtggctacca cagtgaagga agcgctggat gcggcagaac aaatgttgaa ccagcatcga
    35941 tttggggata gtagcaaaaa aatcgtcgta gaagagtttt tggcaggaga agaattctcc
    36001 cttttggcgt ttgtccgtaa taccaaggtg tatccgatgg tcatttcgca agatcataag
    36061 cgggcatttg ataacgataa agggccaaat acaggcggaa tgggtgctta ttcgccagta
    36121 ccgcaaattt caaaaaagat gattgaaaaa gctgtggaag aagttttaaa accagcggct
    36181 ttgggaatga aagaactagg acgctctttc acgggtgttt tatatgcggg tttgattgct
    36241 acgaaggaag gaccaaaagt gatcgaattc aatgcgcgtt tcggtgatcc tgaaacgcaa
    36301 gtcgttcttt ctcggttgaa gagtgacttt gcgcaagtaa tcgatgattt attagagaat
    36361 cgtacagtag agttaaagtg gcagcaagaa ggatacaatg taggcgttgt ggttgcagct
    36421 gccggttacc ccgaagaata tgagacagga atggttctac cagattttct ggaagaagag
    36481 ctttctgttt actatgctgg agtttcctca aataacggca aactagttgg agcaggagga
    36541 cgtctttatt tagtagaagc atacggtgag acgatcaaag aagcacagaa aaaaatttac
    36601 accgcgttaa tggataaaac gacagatgga acattttatc gtacagatat tggagaaaaa
    36661 gctgccaata gcgaactgat cgtgaagtaa atcagctgct tccatttata aaaaatacaa
    36721 caaacataat cggaagcgcc gattttagtg aagccttttc ataaagagtg tggtacactt
    36781 ttgttgtgta tataaataag gaggagttca gatgaaaaaa attcaattag gtacttctga
    36841 tcagaaagca gctgcagtga tcttaggctg tatgcgtatc aacaatgcgg aagatccagt
    36901 aaaagtgatt gagacagctt atgaaaacgg cattgatttt tttgaccatg cagatattta
    36961 tggaaacggt gaatgtgaag aaatctttgc agatgctcta gcaaaaacat caatcaaacg
    37021 agaagatctt tttattcaaa caaaatgtgg aatcgttcca ggtgtgatgt ttgatttttc
    37081 taaaaaacat atcattgaat ctgtagaaaa aagtttgacc cgtctgaaaa tggattatgt
    37141 ggatgcttta cttcttcatc gtccagatac attggtagaa ccagaggaag tagctgaagc
    37201 gtttgatgaa ttggaacggt caggaaaagt tcgttatttt ggcgtcagca atcaaaaacc
    37261 aatgcaaatc gaattgctga aaaaatatgt tcgtcaacca ttagtagcta atcagctgca
    37321 atttggcttg aaacatactg gaatgatcga tcaagggatt catgtcaata tgacagatga
    37381 agcaagtatc gatcatgacg gtagtgtact tgattattcc cgattgcatg atatgacgat
    37441 ccaagcatgg tcaccatacc agtatggatt ttttgaaggt gtatttatcg ggaacgaaaa
    37501 attccctgaa ttaaaccaaa cattagaaga actagcagct aaatatcaaa cgacaccaac
    37561 tggcttggct tctgcttgga ttttacgcca tccagccaat atgcaagtga tcgccggtac
    37621 gatgaacgta gggcgaatca aggaaatcgc tcaagcttca gaaattaata tgtctcggga
    37681 agattggtac gaattgtacc gtgctgctgg aaacatttta ccttaaagat tgtcataagc
    37741 aggtcaaaag ggctataaat taaaaagaca gaataacttg aaagctgttt tcagcttcga
    37801 gatattctgt ctttttttat tgcaaatgga aagagtatca ttgtacggat ttcgtttatt
    37861 agttattttt gtttttaagg aaaagctgtg tccgatattc ttttggagaa atcccttgtt
    37921 ttttcttaaa taaataactg aaatagttag ggtcattata accaatttca aaagcaatat
    37981 ctgaaatacg atagtctgat tgctgcaata atttttttgc taattcgatt ctttgttctg
    38041 tcagataatc gatgaatgtt tggttaaacg attgagaaaa aatcgtactg aaatgagagg
    38101 gacttaatgc aacttgttga gcgacccaag ttaaggacat atctggatcg gtaaaatttt
    38161 ctttgataaa atccaatgca tggttcaaaa ttggctgata tttgttcctt gaaggctgtg
    38221 agggcagagc agttaagaaa gaaatgaaca tataaactgt cgaacggtaa agcgagtcgt
    38281 cggcgctgat tgctaagcgt tgactggtgg atgtgcaatg aaccatttct tgcgggattt
    38341 tcttttcttt caggcagaca tgctggagta gcttagtcaa gacaaataaa cgatataatc
    38401 gtgttcgagt tggtgtgcct tgtggtttcg tcaagtgttc gatgtactta gatacttcgt
    38461 ttttcgataa tgaagacaaa tcaagtgaga ggtcaaagta aaagacagaa tcaactggaa
    38521 gtttcgcttc ttgaatcaaa tcctcatagc tgataatttg ttctttaagg gaactggcgg
    38581 tttcaagtat catctgcgtg atctcaaaag atgcaggtaa ttcgctgatt ctttctgcga
    38641 caggaccaat ggcgacagaa aaattatttt tttgtccctt ttctaattca tggaccaacg
    38701 tttgggctac ctgataactg gtctcgatca cattttcttc aagagaatca actattaaaa
    38761 atttgatgaa tcgagaggaa acagcagaga agatcacttg ttgattctcc ctaaataaaa
    38821 acttcaaata atcactgaga tgagcgtaat cttcaaagtc tgtactgact tggtttgctg
    38881 ctaataatac ggtcattttt ttccctgcta aagagcgatt gaaagattca gcaagtttca
    38941 atgtttcact gatcgtaagt gagcctttga acaaaccgtt caagaaatga ttttttttca
    39001 attcgaaaac aagatcagaa gaagtgtgcg tttctttttg ctgcaaatct aaagcagtta
    39061 ctgctttttc tattgcttgt ttcaattcgc tacttttaat cggtttaagc agataatcat
    39121 ctgcctgtac ctggattgct cctttcatat aatcgaaatc gtcgaatcca ctaataaata
    39181 tgagacgagt ccaaggaagt agtttccttg cttcttttga aaaagtcaac ccgtccatga
    39241 aaggcatttg gatatcagtc agaatgatgt cgggctgtaa gtcgataatt gaagacagtg
    39301 ccatctcacc atctccagct tcgccgacat aagtgattgg ataataagaa ccaaggacag
    39361 ttatttgttc ccgcaagtta ttgcgaatga ggtattcgtc ttcgatgata aaaactttat
    39421 acatgtttgt ttcctccaat tgttttggga gatagaggga tcatcagtgt gacagttgtt
    39481 ccttctcggt atttgctgtc aatcgtgata ggtttttgat catttccgta ataaagaagg
    39541 agacgcttgt ttacgttgta aagtccgtag ccgatactga aatctgaatc gatccctttg
    39601 gctaactctt tacgtatttc tgccagtttt tctgcaggga tgccaatccc gttgtctata
    39661 actttgaatt ggatcatctc atcaattttg catgtggtaa tacgaataaa tcctttgtga
    39721 cggacgtact tcgttccatg gtatacagca ttttcaatca atggctgcaa gatcattttt
    39781 aaaatagaaa caggaaaaag tgtttctggt atatcgatct tatagtcaag catatggccg
    39841 tatcttattt ttaagatgac tagataatca ttgatatggc taatttcttt ttcaagagga
    39901 atccagtctt gtcctttgct taaagacgta cgaaaaaagc cggaaagtgc atgggtcata
    39961 gtcagagctt cttcaaagca gtcttgttcg atcaaagaaa taatggcatc caaactgtta
    40021 taaataaaat ggggagtaat ctgagcttgc aatacacgaa cttcactttg tgctaaatgg
    40081 tattgtttca aagcgttctc ccgtaaagac tggtttagat cttccgccat tttattcatg
    40141 ctagcggtaa gtgtttgtaa ttccagtgtt tccggcaggg ttaaacgaaa gccaaggttc
    40201 cctgaagaaa gttgttctga catctcgatc agctgattga tcggttcttg gacctttctc
    40261 tctaaaaagc gacgattctt tttcgcatag aaaaaaatgg tactaagaac tccaagttcc
    40321 actattgtta atagaatgag cgagcggaca agtgtcgtat tttttttgct tgctaagtcg
    40381 atctccacac gaacaaaatc ttgtaagata tctgacagca gttgagtgac agaatctacc
    40441 tgattgatga tctcttcatt ggtatccata aaatcttttg ccaagatatt attgatcagc
    40501 tcgttttgat aatcttccag tgtatcgatc gtacgtagcg atacatctaa cgtactaatt
    40561 tctttaagtg tatttgtatt atttttgatc tctcgaatat cattcctaag ttcttctagg
    40621 atattttcct cattgaactc atctacggtt acttggccag aaatcaagtc ccacatactt
    40681 tccaaaactt tttgatcaac cttcaaagca attgcatctg ctttttggat atttgcgatc
    40741 gtctgttgat aaatcaaaag ctgttgcgaa tataacagta tgctcagtag caaagggata
    40801 acagcaaata gaagcatggc tttgtttgct tgggcaaaaa tctgttttat gcttaaattt
    40861 tttgtcatga tacttctcca gtcaataatt tctggttggg atgttagcta tattctgctg
    40921 ggaaaagaca gtctcggaaa taaaaatatc tttaggtaat gtttttcctg tttgaatatt
    40981 tccaaaataa cgagtaacgg tattggctac ataccagccg acatcaggtt tacactcgac
    41041 tacacaattg acgattcctg agcgtagctt ctcgatcata tcttcttgtc catcaatagt
    41101 tacaatgatc aaatcttctc caggtttgat tgatgtttct tttatcgcag ctaaagcacc
    41161 gtgagtcatt tcatcactat ggctgtataa tacatcgatt tcggataaat catttgtttc
    41221 taaatatcgt ttcatctggt cttttccttt catccgaata aaatcaccga caattcggtg
    41281 aacaatagtc atcggaggtt tatggtcgat ggttccatct ttttttaggg ttggtttacg
    41341 attgattgat tccaaaaatc cttcggagcg tagcttggtc ggtgtggagt tctctgaacc
    41401 agcaagttct aggattctga tttcctgttt ctttgaattt gtgaagtgat tgctgacaaa
    41461 taaacccgcc cgatttcctt ctgctttgaa actggggcca atgtgggtga cgaaaagctc
    41521 gggatcattc gtttgaatat gtctgtcgac aagtatgaca gggatccctg ctcgctttgc
    41581 ttcttttaat acagagtccc acccggattc ttgcaaagga gtaaaaatga tcatatccac
    41641 tttataggca atgaacgtcc gtatatcttg gatttgccgt tcctgattca tgtagccatt
    41701 tcggtagaga acttggtaat cttctttttc aagcgcagtg ataattgatt cagtatgctt
    41761 tttccgccag ctactctccg ttcctgattg cgaaaaacca acgactagtg ggtcaggtgc
    41821 ttcctttgta ttaggtgtac accccatcat ttggtaagcg attacaacaa gggctaaaaa
    41881 aaataaaaca cgtttcactt taaatcactc catctggatt tgttagtact atcttatcag
    41941 ataaaaggga aaatcaaagt caaaaggaat caatcgaaaa aaatccaaga aacttaaaaa
    42001 aatccaagac aaaaaaagta cacctaaaaa atttaaagta aatcccataa aaaaatcaat
    42061 gttaacgctt tctttattag ctatactcgg tgtatcaatt gaaatggggg ataaaaaatg
    42121 aaaaacaaat ttagaaagat tgttgggact atcgctattt tgaccgcaag cattctatta
    42181 agtgcgtgtg gcaattcagg atctgcagat gatagtacgg ggtatgtagg aatcgctatg
    42241 ccgaccaaat cagcagaaag atggatcgct gatgggaaca acatggtctc ggaattagaa
    42301 aaactgggtt ataaaacaga tcttcaatat ggagaagata aagtagaaaa tcaagtagca
    42361 caaatagaaa atatgattac aaaaggggta gatacacttg tgattgcttc tatcgatggt
    42421 tcggctttaa ctgatgtgtt agctaaagca aaagaagcag atatcaaggt gatcgcctat
    42481 gatcgtttac tgatgaattc agaaaatgtc gattattacg cgacttttga taattttgga
    42541 gtaggtgtat cgcaagcagc ttatattgaa gagcacttag gattgaaaga aggaaaagga
    42601 ccattcacga ttgaattatt tggtggctcg cctgacgaca acaacgcact aatcaattac
    42661 aacggagtga tgtctgtttt acaaccatat atggacaacg gtcagttggt cgtgccatct
    42721 ggacagacga gtttcagtca aatcgctacg cttcgctggg atggttctac ggcgcaagca
    42781 aggatggata atctgttaag cgcaaattat actgaccaaa cattggatgc tgttttgtcg
    42841 ccatatgatc caataagttt aggaatcatt tcgtcattaa aaggcgtggg gtatgggagt
    42901 gaaagtaaac ctttacctgt gattactgga caagatgcga cagttgctgg cgttaaatcg
    42961 atcattgccg gggaacaaac acaaacgatc tttaaagaca cacgtatctt agcaaaaaat
    43021 acgattgaaa tgatcaaagc aatcagtgat ggagaagagg ttccggtcaa tgatactgaa
    43081 acttatgata atggggtgaa aactgtccct acgtatttag caaatacagt atctgtcgat
    43141 aaagataatt atcaagcaga gctgatcgac accgattatt ataaagaatc cgatctgaaa
    43201 aattaagagg aaccaaacaa ggaactaaag agatctaagt cagagagagg ataagaatta
    43261 tggcggattg tattttaaaa atggcagata tcaccaaaaa attttctgga atccgagcgt
    43321 tagattgcgt gaatctgatg attgaacgtg gggaaatcca tgcattgtgc ggagaaaacg
    43381 gagcgggaaa atcaacattg atgaatgtct tgagcggctt gtatccatat ggctcctatg
    43441 acggggaaat cttttatgat ggaaagctgt gtcaatttaa agatttgaaa gatagtgaag
    43501 aaaaaggcat cgtcatcatt catcaagaat tagcactaag tccttatctt tcaataaaag
    43561 agaatatttt tctcggaaat gaacaagcag aaagaggcgt gattgattgg gaactgacag
    43621 aaaagaaaac atatgaactg ctgaataaag tcggtttgtc tatcgacccg aatcttctag
    43681 tatcccagat tggcgtaggg caacagcaac tagttgaaat agctaaagca ttatcaaaat
    43741 ccgtccgtct gttgattttg gatgagccta ccgctgcatt gaatgaagaa gaaagcgcga
    43801 atttgctgag attgatccga gagttgaaag agcaaggtgt aacctccatc attatttcac
    43861 ataagctgaa tgaaatcgtg gatatcgcag atcgaatcac cattttacgg gatggacaga
    43921 cgattgagac actagaaaat gaaatgatca gcgaagagca tattattcgc ggaatggtgg
    43981 gcagagattt agttgatcgt tatcctgaaa gaaaaccgga tattggagaa gtttatttta
    44041 aagtagaaga ctggacggtc tatcaccctc ttgatcacga gcgaatcata aacgaccaca
    44101 tcaacttgca tttgaaaaaa ggagagattg taggaatagc tggactgatg ggagcgggca
    44161 gaactgaatt cgctatgagt ctatttggac attcttatgg aagcaagata tccggacgta
    44221 tattaaagga aggggcagag atatcagtta agaccgttcc ttcagccatt gcaaacggac
    44281 tcgcttatgt ctcggaggac cgaaaatctt taggactgaa tctgctgatg gatattcggg
    44341 aaaacgtgac gctgagcagt ttggataaaa taagtcaaaa aggagtatta gataaagaaa
    44401 aagaagtaac tgaggcagaa aatttccgga aaaagatgcg tataaaaaca aactccatcc
    44461 atcaaattgt cagtagtctt agtggtggga atcaacaaaa agtcgtttta tccaagtggc
    44521 tgatgacaca acccgatatt ctttttttgg atgagccaac ccgtgggata gatgttgggg
    44581 caaaatatga gatctacacg attatcgaag aaatggcagc aaatggcaaa gctgtctgtc
    44641 ttatctcttc tgaacttcct gaaatattag ggatgtgtga tcgaatctat acgatgaacg
    44701 aaggacgtat tacaggagaa gtctcgcgag aagatgcaga tcaggaagtc ttgatgaaat
    44761 taatgacgaa agaagaggtg ggttaagttg gaacaggcaa aaaatcttac ggataaaaaa
    44821 gagaaaaaac taaatttgaa aagtagagca gctgatcttt ttggtcgcta cagcatggtg
    44881 atcattttag cgggactact tattgcgttt caattgatga cggatggtat tttctggcgt
    44941 ccattgaata taactaacct tgttctgcaa aatagccata tattagtact agctgcaggg
    45001 atgttattag ttgtgctctt aggtcatgtg gatttgtcgg tagggtcagt gatggcgttt
    45061 gttggtgcaa ttgcgggcat gatgatggtc aatcgccaaa tgaacccgtg gatgacagtt
    45121 atcgcatgtc ttgcagtagg cgggctgatc ggcgcatggc aaggtttttg ggtagcttat
    45181 gcagggatac cggcgtttat cgtgacttta gcaggattgc tgatgtttcg tggactgaca
    45241 caagttgttc taggaggcca atctctagca ccttttccag ctgttttcca aaaaatgtct
    45301 acgggttatt taccagactt aagcgaagga agcagccttc acttcttgac attgatttta
    45361 ggaatgatca tcacgatcgt gttagttgtt ggtcaatggc gaacaagaaa tcgtcggaaa
    45421 aatcatttat ttgaagtgga atctccaaca gctttcttgg taaaggcggt atttacagca
    45481 gtcgttgtac taggaataac ttatatcttt gcctcttatc aagggtatcc gattattttg
    45541 atcattcttg gtttgatcgt tgcagcatat gggtttttga caaataaaac agtagcaggt
    45601 cgacagattt atgcgacggg cggaaatcga aaagcggctg aactatcagg gatcaaaaca
    45661 aagcgaatca cgttctgggt attcgtcaac atgggagtca tggcagcttt agctggatta
    45721 gttttagctg ctcgattgaa tgcggcaaca cctcaagcag gtacatcgat ggaactggat
    45781 gcaatggcgg cagtatattt tggtggtgct tcgacttccg gcggcattgg aacaatcatg
    45841 ggtgcgattg tcggtggctt ggtcatgggt gtattgaaca atggcatgtc gattttaggc
    45901 gtcggggtag actggcagca ggcaatcaaa ggattgatct tgcttttagc cgttgtgcta
    45961 gacttgtata ataagaaaaa acgaacaacg taggagaacg ccggttattc agaaaaaaga
    46021 ctgtgacaag acatttgtca cagtcttttt ttcgctaaaa gcgaacattc attgtttcgt
    46081 ttgtatagac taaaaataat atatgttttt ttaccattcg tataaattct ctttttgctc
    46141 ttgatttttt tgcttaattg agttaaatta catagggcgc taaaaaaagc cttgattaaa
    46201 ttaataatgt tcgtgttttg ctggatgtga acatcaggag gataatgtaa tgaaagtatt
    46261 tgattacgaa gatattcaat taattcctaa taaatgtatc gtaaatagcc gttctgaatg
    46321 tgatacaacg gtcacattag gtaaacatac attcaaaatg ccagtagtgc ctgccaacat
    46381 gcagacgatt attgatgaaa caatcgcaga attcttagcg gaaaatggtt atttctatat
    46441 catgcatcgg tttgatgaag cagctcgaat tccgtttatc aaaaaaatga aaaagcgtgg
    46501 attgattacc tcgatcagtg ttggcgtcaa gaaagaggaa tattccttta tagagaagtt
    46561 ggcagaagaa tcgctaaatc ctgattatat cactatcgac atcgcgcatg gacatgctaa
    46621 ctcagttatt gatatgattc agcatattaa aaaatatttg cctgaaactt ttgtgattgc
    46681 cggtaatgtt ggtacaccgg aagccgtccg tgaattggaa aatgcgggcg cagatgcaac
    46741 aaaagtcggg attggtccag gaaaagtatg tattacaaaa atcaagacag gctttgggac
    46801 tggaggctgg caactggcag ctcttagatg gtgtgcgaaa gcagctcgca agccgatcat
    46861 cgctgacggt gggatcagaa cacatggcga tattgcaaaa tctgtccgtt ttggtgcgac
    46921 aatggtcatg attggttcat tgtttgccgg tcacgaggaa tctcctggcg aaacaaaagt
    46981 cgaaaatgga atcgtgtata aagaatactt tggttctgct tcagaattcc aaaaaggcga
    47041 aaaacgaaat gttgaaggaa agaaaatctg gatccaacac aaaggatctt tgaaagatac
    47101 actagtggaa atgcaacaag accttcaatc atctatttct tatgctggag gaagagatct
    47161 ggaagccatt cgaaaagttg actatgtcat cgtgaaaaat tcgattttta atggagacac
    47221 aatctaaaaa ataaataaaa tattaggatg ttgtgaaaaa gttgtcctgc attaagtaat
    47281 aagaacagcc aaacaaaaat agttctttat atgttttgtt tggctggaat tattatcgca
    47341 gatacaagct tttgaacatc attattcaga ataagggact gtgataagat ttttgtcaca
    47401 gtcccttatg ttttgcaaaa aaagtttttc ttttttctcg taaattcggt aaaatcaaaa
    47461 gaaaaaatag acaatagaaa agagaagctt atgtgggaaa aagtagagaa aaaacaagga
    47521 ccgatttatc gtcaaattat ggaacagatt atgagagaaa tcgaaaacgg aaggatcaat
    47581 cctggaagtc aacttgaatc agaacgaaaa ctagggcaac tttttggtgt gaatcgttcc
    47641 accgttgtcc atgcattgga agagcttagg gagcttgggg tactaacgag caaacgtggc
    47701 agcggatggt ttgttaatca gactgaatgg gggaaattcg ctgttccaag aatcgattgg
    47761 cggcaaatga tctctcctcg ttatgagcag acggactggt atgagcaaaa aataagagaa
    47821 gcaaagcaga tccaaaaaac atcgtttctt gatttatacg ctagcgagat gccagatgag
    47881 cttttgccga attttgagtt tccttcttat tctcttgaag aaatcttagc ggaagaaaaa
    47941 gaaatggatc ttttaggcta tactccgtta cgtaacaaaa tcaaacggta tttggaaagt
    48001 aaatttgaat tggcattttc ccccaaccag cttcttgtga ctggtggcgg tcagcaagca
    48061 atctttttga ttttacaaac gattctttca gctggagagg ggatagctgt agagtcacct
    48121 tctttttttt accgattacc tcttttcaaa gcttcaggaa cgcgtttatt tgggataccg
    48181 atggatgaag aagggatcga cttagaaaaa ctgaaagaat cgatccataa aaataaattg
    48241 aaagcagtgc ttatcaaccc gaattttcaa aaccctacag gaaaagtgat gagtcaaaaa
    48301 cgacgagaaa agctagtaga attgtgtcga cagtatcggc ttccaattat tgaagacgat
    48361 gtatttacgg atctttcatt tctaaaccaa gaagaagtcc ttcctatacg aacgattgat
    48421 ccggataatg tgttatacgt cggttcgtta tctagggttt taggaaaaac aacgaaaatc
    48481 ggttgggtga taggacctga gacttttatc tcgcaactag ctcaagcgca agaaatgatg
    48541 gagttttcga tgaatatcct tacacaaatc acagtggcta atgtatttaa tgaagcaatt
    48601 gatttgaaaa tgcaagatgt aagaaaaaag ttgaaagaaa aaagccgact gataagcgct
    48661 tggggagatt cacaatcgtt tttcaagctt cattcaattt taggtggtta ctatgcctgg
    48721 attacttggg atgggaaaaa actagacccc gaactagcag aaaaagtagt agatatgggt
    48781 cttggaattg caccaagtct actttttggg caagatataa atggaatcag aatcaattac
    48841 agtaggctgg atgaaaacca aataccgttg ttttattcaa agatgaaaat tctagaaaag
    48901 tggttaacag aataatttca caaagtaaat agattaaaga atataacagc ttggatttaa
    48961 atttcaagct gtttcttttt tttcggaaaa agaatacgat ataaagaaac taagaaaaaa
    49021 ccattatttg tacgtccgtt cacgatgtgt aaataaaata aatgtttgca acaaaactgt
    49081 ataacaccaa cgtttttaaa tagaaacaga tggttgtaag tttgattaga aaatagaaaa
    49141 aatgcagatg aaatataaaa cgctttcctt attgtgattt tattcactaa tgctgttaat
    49201 atgcggattg gcataatttc ctttcatcgt gaaaatttgt gcttttagct tgtatttctc
    49261 ataaatccat gataatttat taatgaagac ggttaaatag tgatacagtt ttattcactg
    49321 tttcaatcgc agaatgaaga gattttctta tagatgagag aggaatgtgt gacaaatggc
    49381 aaacaaaaaa gcaccgatcg acttccaagc actgctagat gaagtcaatg cagatttccc
    49441 agtatatcaa gcacttgatc aagatggaaa cgtagttaac aaagatttag tacctgattt
    49501 atccgatgat gaactcgttg aattaatgac tcatatggta tggtctcgtg tattagacca
    49561 acgttcaaca gcgctgaacc gccaaggacg cctaggcttt tttgcaccaa ctgctggaca
    49621 agaggcaagt cagctggcta gtgcttacgc atttgataaa gaagatgttc ttcttccagg
    49681 ttatcgggat gtacctcaac tagtaaaaca tggtttacct ttatctcaag cttttttatg
    49741 gtctcgagga catgctgcag gtaattctta tccggaagaa ttgaaagctc taccaccaca
    49801 aattatcatc ggtgcacaat atgtacaagc agccggtgtt gcattaggtt tgaagaaacg
    49861 aaacaagaaa aatgtcgtct tcacttatac tggtgatggc ggttcatcac aaggtgattt
    49921 ttacgaagca atcaatttcg ctggcgctta ccatgcaaat gccgtatttt atatccaaaa
    49981 caacgggtat gcgatctcta cacctcgtga aaaacaaact gcagcaaaaa cacttgcaca
    50041 aaaagctgtc gcagctggga ttcctggtat tcaagtcgat ggaatggatc cgcttgcagt
    50101 atatactgta tctaaaatgg cacgggaatg ggcgatcagt ggaaatggac cagtcctaat
    50161 cgaaacactg acttatcgtt atggaccaca tacactatct ggtgacgatc caacccgcta
    50221 ccgtacaaaa gacacagatg acgattggca gaaaaaagat ccattgatcc gcttccgtaa
    50281 atatttgacg gaaaaaggac tttggtctga agaaaaagaa gaacaagtca tcgaagcaac
    50341 taaagaagaa atcaaagcag ctattgctga agccgacaaa gtaccaaaac aaaaggtttc
    50401 agatttcttg aaaaacatgt tcgaagtatc tccacaatca atcaaagaac aaattgcaat
    50461 ttatgaagcg aaggagtcga aataatcatg gcacaaaaaa caatgatcca agcaatcaca
    50521 gatgccttag cacttgaact agaaaatgac gagaatgtcg ttgtttttgg tgaagatgtt
    50581 gggaaaaacg gaggagtttt ccgggcaact gaaggattgc aggaaaagtt cggagaagac
    50641 cgagtattcg atactccttt agctgaatct ggaatcgcag gattgagttt cggtcttgcg
    50701 ttagaaggat ttcgtccagt tcctgaaatc caattctttg gttttatttt cgaagcaatg
    50761 gacgaagtag tagctcaaat ggctcgtaca agatatcgaa tgagcggcac tagaaatcta
    50821 cccatcacaa ttcgttcgcc atttggtggc ggtgttcata caccagaatt gcattcggat
    50881 aatttagaag gtttgatcgc tcagtctcct ggtattcgag tagtgattcc ttccaaccct
    50941 tatgatgcaa aaggactatt gatttcttct attagaagta acgatccggt tgttttccta
    51001 gaacacatga aactatatcg ctcattccga gaagaagtgc cggatgaagc ttacgaagtt
    51061 cctttggata aagcagcagt tactcgtgag ggaacagatg tatctattat cacatacgga
    51121 gcaatggtgc gtgaagcaat caaagcagca gacaatcttg caaaagaaaa tatatctgtt
    51181 gaaatcattg atcttcgtac agttgcgcca ttagatgtag aaacgatcat tcaatccgtg
    51241 gaaaaaactg gtcgcgtagt tgttgtccaa gaagcacaac gacaagcggg cgttgccgcg
    51301 caagtagttt ctgagatctc agaacgcgct atcctttcat tagaagcccc aatcggacga
    51361 gtttctgcac ctgatacagt cttcccattt ggacaagcag aaaatgtctg gttgccaaat
    51421 gcaaaagata tcgaagacaa agtaaaagaa atcgcggaat tttaagaaaa gaagggacga
    51481 aacaaagtca tggcttatca atttaaattg ccggatatcg gtgaaggaat cgcagaaggt
    51541 gaaatcgtca aatggttcgt caaaccagga gatacgatca atgaagatga tacattacta
    51601 gaagttcaaa atgacaaatc agtagaagaa atcccgtcac ctgttacagg aacggttaaa
    51661 aatgtgattg ttccagaagg taccgtagca aatgttgggg atgtattagt agaaatcgat
    51721 gcaccaggac acgaagacaa tgaaggggac agtggtgtag ccgcagaatc ccaaactcca
    51781 gctaaaccgg ctgctgaacc cactgttgat acagaatcag ctggatcgtc aagcgaagga
    51841 gtcttccaat ttaagttacc ggatatcggt gaaggaatcg cagaaggtga aatcgtcaaa
    51901 tggttcgtca aaccaggaga tacgatcaat gaagatgata cattactaga agttcaaaat
    51961 gacaaatcag tagaagaaat tccgtcacct gtcacaggga cagttaaaaa cgtgattgtt
    52021 ccagaaggca ctgtagcaaa tgttggggat gtattagtag aaatcgatgc tcctggacac
    52081 aatagtgcac cgtcaactag tgcgccatca gctgaagcac caaaagaaaa agtggaaaca
    52141 tcaggttcag caagtgtcgt tgaagctgcc gatccaaata aacgtgtatt ggctatgcca
    52201 tctgttcgtc aatttgcccg tgaaaaagat gtagatatct cacaagtaac tgcaactgga
    52261 aaaggtggca gagtaacaaa agaggacatc gaaaacttct tggcaggtgg tccaagcagt
    52321 gcaccagcga aatcagaagc gccagaagcg gcagcaccga aagaagcagc tcctgcagca
    52381 gaaagcaaac cagctgcacc agcaaaacca ttcaagtcca acttaggaga cttggaagaa
    52441 cgtgtagcaa tgacgccaac acgtaaagct atcgctaaag caatggtcaa cagtaagcat
    52501 acagctccac acgtgacatt gcatgatgaa gtagaagtgt ctaaactatg ggataatcgg
    52561 aaacgattta aagaagttgc tgctgcaaat ggtacgaaac ttactttctt gccatatgtc
    52621 gtgaaagctt taacagcgac tgttaagaaa tacccagtat tgaatgcatc tatcgacgat
    52681 gcaaaccaag aaatcgttta taaacattac tacaatatcg gtattgcaac tgatacggat
    52741 cacggactat atgtaccaaa tgtgaaagat gcagaccgca aaggaatgtt tgctattgca
    52801 gacgaaatca atgagaaagc aaaattagct catgacggca aactatctgc agaagatatg
    52861 cgcaatggaa caattacgat cagcaatatc ggatctgtag gtggcggttg gtttacacca
    52921 gtcatcaact atccagaagt agctatttta ggtgtaggaa cgattgcaca acaaccaatc
    52981 gtaaatgctg aaggcgaaat cgttgtcggt cgtgtgatga aactgtcatt aagtttcgat
    53041 caccgtatcg tagatggagc aactgcgcaa caagcaatga ataatatcaa acgtctttta
    53101 gctgatccag agctattaat gatggaagga tgataaacaa atggtagttg gagattttgc
    53161 tgtagaatta gatacagttg taattggtgc gggacctggt ggctatgtag ctgccatccg
    53221 cgctgctgaa atgggacaaa aagtagcaat cattgaaaga gagtatattg gcggtgtttg
    53281 tttgaatgtc ggctgtattc cttcaaaagc actgatagct gcaggacacc attatcaaga
    53341 gtcactagat tctgaaatgt ttggagtaac tagtgaaaac gtcaaactag attttgcaaa
    53401 aacacaagaa tggaaagaaa acaaagttgt ccatacgttg acttctggtg ttggtttcct
    53461 attaaaaaaa cataaggttg aaacaatcga aggagaagcg ttcttcgtag atgatcacac
    53521 actacgcgtc attcatccag attcagctca aacttattcg ttcaataacg caatcatcgc
    53581 aacaggttcc cgtccaatcg agattccagg atttaaattc ggaggacgtg tgttagattc
    53641 tactggcgga ttagcattga aagaagtacc taagaaattt gtgatcatcg gtggcggtgt
    53701 gattggtgcc gaattgggcg gagcgtacgc aaacttagga gcagaagtaa cgattcttga
    53761 aggctcacca caaattttac caacttacga aaaagatcta gtaaaattag tagaagatga
    53821 tttcaagaaa aaaggcgtaa cagtcgtaac gaatgcaatg gcgaaagaag cagtcgataa
    53881 cggtgacagc gtgacagtga aatatgcagt ggacgggaaa gaagaatccg ttactgctga
    53941 ttatgtgatg gtcactgtag gtcgtcgtcc gaatacagat gatatgggct tagaacaagc
    54001 gggtgtcgaa gtaggagaac gaggattgat cacggtggat aaacaaggac gtacgaacgt
    54061 acctaacatc tatgccatcg gtgatatcgt tccaggggcg gcattagcac acaaagccag
    54121 ttatgaagca aaaatcgctg ctgaagcaat ctctggcaaa aaagtagctg tagattataa
    54181 agcaatgccg gcagtagcct tcactgatcc tgaattggct tctgtaggta tgacgatcaa
    54241 agaagcaaaa gatgctggta tcgaagcaaa agcatataaa ttcccatttt cagggaacgg
    54301 tcgggcatta tcgcttggta aaacagaagg attcatccgt ctagttacga cgatcgaaga
    54361 taacgtgctt attggcgcgc aaatcggtgg agtcggcgca agtgatatgg tctctgaatt
    54421 agctttagca atcgaatcag gaatgaatgc tgaagatatc gccttgacga ttcatcctca
    54481 tccatctctt ggcgaaatcg tcatggatgc ttcggaatta gcattaggtt taccaatcca
    54541 tatttgattt gaataataca ggcgaaacgc aactgaaaaa tcagttgcgt tttgtttttt
    54601 agaattatca ataatattat tatgtaaact taaaaatatg acaacttctt ggtttattac
    54661 cacttcctat gctacactga agaggaatat gaaataggag agttgaagat gcaagaatat
    54721 cagtatccac ttgatttaga ttggacaaca gacgaaatgg tcattgtaat gaagatgtgg
    54781 gaagatctgg aacgtgcgaa tgaaaaaggg ataaacaatg aagctttttt gaagacttat
    54841 cagcagttta aaactgttgt caaatccatt ggtgaggaaa aacgattggg acgggaattt
    54901 gaaaaagctt ccggttattc tttatatcgt acagtgcagc aagcaaaaaa aaatccaaac
    54961 aaaactttga gaatgaacgg gtgagataaa tggaacagat gattgatcat atcaagtcct
    55021 ggcttgaaga agctggtgag aaaatcaaat cttctcaaca aacattaaca atctcgcaaa
    55081 aatcaaatcg aacggatcta gtaacaaatg tcgatcgaaa catccagaat tttctaatcg
    55141 aaaaaatcca ttcctttgat ccagaggcga aaatattggc agaagaagaa gggtgtaatc
    55201 aattatcgga tctcgatgga agagtcttta tcatcgatcc tattgatggc accttgaatt
    55261 ttgtgattga aggggaaaat ttttgtatta tgctcgctgt ttatgaatcc ggcgttggaa
    55321 agcttggttt catttatgac gtgatgagaa atgaactttt ttggggtgga aaaggaatag
    55381 gcgtgtataa aaatgatgag ccattgccta gaccagcaaa taaatcctta tcggaaggct
    55441 tacttggtgt gaatagttat cttttcggtc ataataaata tggaatccga gaaattgcgg
    55501 aaagcagcat ggggacacgt atgtacggat gcgctggtct tgaactagtg gcaatcttga
    55561 aaggaaacca cattgcttat atttcaaacc ttagcccttg ggattatgca gcgggaaatg
    55621 tcttattgga agagtttggt atggtaacaa gcggtctttt tggtactcct ttgacatttg
    55681 aaaaaaggga atactattta gcagggactt cttgtgcata tgcagaaatc gcagaccttt
    55741 tgaaaaaaga aggctgaaaa aaaggtagaa atcattcaca aaaatgataa aaacgagcaa
    55801 aagattaatt ttctcgaaaa aatgaaaaca ttttactata tttcatgaaa aaagtctgct
    55861 ataattagca gtcgagtaaa atttgccctg tcataaagaa ccaataaaaa aggagttagt
    55921 tacattgaat tatagaaacg atatccgtaa cgtcgcaatt attgcccacg ttgaccacgg
    55981 aaaaacaaca ttagttgatg aactattaaa acaatcagat acattagacg cacatacgca
    56041 attgcaagaa cgtgcgatgg actcaaatgc gctagaaaaa gaacgtggta tcacgatttt
    56101 agccaaaaac acagctgtag actataaagg tattcgcgta aacatcatgg atactccagg
    56161 acacgcggac ttcggtggcg aagtagaacg gatcatgaaa atggttgacg gcgttgtgtt
    56221 ggtcgtagat gcgtatgaag gaacaatgcc tcaaacacgt tttgtattga aaaaagcact
    56281 ggaacaacat atcacaccaa tcgttgtcgt aaacaagatt gacaaacctt ctgctcgtcc
    56341 tgagcatgtc gtagatgaag ttcttgaact attcatcgaa ttaggagcag atgatgatca
    56401 gttagacttt cctgtgattt atgcatctgc actaaatggt acgtcaagct tgtcagatga
    56461 tccagcagat caagaaccaa ccatggcacc tatttttgat acaattattg aaaaaatccc
    56521 tgctcctgtc gataacagcg acgaaccatt acaattccaa gtttcattac ttgactacaa
    56581 cgattatgta ggtcgtatcg gaatcggacg tgtatttaga ggaacaatca aagtaggaga
    56641 ccaagtagca ttaattaaat tagacggaac agttaagaaa ttccgtgtaa cgaaattatt
    56701 tggtttcttt ggactaaaac gtctagagat tcaagaagca aaagctggag acttgattgc
    56761 tgtctcaggt atggaagata tctttgtcgg tgaaacagtt actcctgtgg atcatcaaga
    56821 tgcgttacct atccttcaca ttgacgaacc aacattgcaa atgactttct tagttaataa
    56881 ttcaccattt gccggtcgag aaggtaaatt cgtaaccgct cgtaagattg aagaacgttt
    56941 gatggcagaa cttcaaacag atgtatcctt acgagttgaa ccaacaaact ctccagatgc
    57001 atggacagtt tctggtcgtg gagaattgca tttgtctatc ttgattgaaa acatgcgccg
    57061 tgagggatac gaattgcaag tatctcgtcc agaagttatt gaaaaagaaa tcgacggtgt
    57121 aaaatgcgaa ccgtttgaac gcgtgcaaat cgatacacca gaagaatata tgggtagtgt
    57181 tatcgagtcc ttgagcttac gtaaaggtga aatgcaagat atggtccata caggaaacgg
    57241 acaaatccgt ttgacattct taacacctgc ccgcggtttg attggttatt caactgaatt
    57301 cctttcaatg acacgtggtt acggaatcat gaaccatact tttgaccaat atctgccaat
    57361 gcttcctggt caaattggcg gacgtcacca aggggcgctt gtttcgatcg atacaggaaa
    57421 agcaacgact tattctatca tgagtatcga agaacgcggt acagtcttcg tagaaccagg
    57481 tacagaagta tatgaaggaa tgattatcgg ggaaaacagc cgtgataatg atttgactgt
    57541 caacatcacg aaagcaaaac aaatgacaaa cgtacggtct gctactaaag accaaacatc
    57601 tgtcatcaaa aaaccaaaac aattgacgtt ggaagaatct ttggaattct tgaatgatga
    57661 tgaatattgc gaagtaacac cagaaagcat tcgcttgaga aaacaaattt tagagaaaaa
    57721 tgctcgtgaa aaagcatcta aaaagaaaaa ataatctgta aaaggatgaa gagagaaacc
    57781 agcttgttat aaaaaatagc tggtttcttt ttttgtccaa ctcttttctg tggagaagtc
    57841 atctttgtca atggacataa attcagaaaa tacgccgaaa gacgtttaaa agacttttaa
    57901 aaaacttatt tctagagagt ttttgtttca taataagata atgtttcacg aaaggagatc
    57961 aacttgtcct tcgaagaaaa tcgaagtaaa atggcaagag aaaggagcgg gaagatgaac
    58021 gacttattaa aacaatactt tggctatgat gagtttagac ctggtcaaaa agaaattatc
    58081 cagaaagtca ttgatcagga aaatgtatta ggaatcatgc ctaccggaag tgggaaatca
    58141 atctgttacc agcttccagc tttactatta gatgggctta ctgtggttgt gtccccttta
    58201 atctctttga tgaaggatca ggtggacgcg gcgaatcaat taggtatacc cgccactttt
    58261 atcaatagct cgttagatgg ttacgaaaca gctagacgct tccaagaaat cgatagacag
    58321 cagtaccgtt tactttatat cgctcctgaa cgttttatca tgccggattt tatccaagca
    58381 atgaagcgat ggaacgtccg tatgatcgct attgacgagg cacattgtat ctctcagtgg
    58441 gggcatgatt tccgcccaag ttatttacag atggcgaacc agctagatca actgccaaat
    58501 cgtcctgtaa ttgttgcttt gacagctaca gcgactgtgc aagtagctgc tgacataaaa
    58561 agacttttaa aaattccaga aaacaaccat attcagactg gttttgaacg agaaaatcta
    58621 cgatttcaag taattaaaga tcaaaaaaaa gaacaatatc taatcgaata tttaaagatc
    58681 aacaaaaatc aatctggtat catttatgca gcaacgagaa aagaagtaga ccgtctctat
    58741 catttgctta aaaaattcga tttttccgtg ggtagatatc atggtggtct aaatgagaat
    58801 gagcgaacag aaatgcaaga agccttttta tacgatcgct tgcagctgat tgtagcgaca
    58861 aatgcatttg gtatgggtat aaataaaagc aatgtccgtt ttgttatcca ttatcaaatt
    58921 cctggctctc tagaagcata ctatcaggaa gctggtcggg cgggaagaga tggtctttcc
    58981 agtgaagcca tcctgctttt ctctcctcaa gatatccaag tccaaaagtt ttttatccag
    59041 cagtcccaaa gagaagaagg gcaaaagcaa aaagaatatg aaaaattaaa agcgatgaca
    59101 gaatacgtgt atattgagtc ctgtcttcag caatacatcc taaactattt tggcgagaca
    59161 agcagtccgt gcaaccgttg tggtaactgt ttagatgaca gagaaatagt agaagtaacg
    59221 acagaagcac agatggttct ttcttgctta aaacgtatgg gtgagaacta tggtaaacaa
    59281 atgctgatga aagtcttagc aggatcgaaa gaacaaaagc tgcaagcttt aggttttgga
    59341 catttatcta cctatggact gatgaaaaac caatcgcaaa aagaaacgat gcagttaatt
    59401 gaatatttga tatcgaatgg ctatcttctg accatcaatg gtgagtatcc tgttttgaaa
    59461 gtaacagaaa gagggataca agtattgaaa ggacaagaat ctgtttatcg aaaggaaccc
    59521 aagaaagtcc agcagctatc ggatgaagaa accgatacac tatttgaagt gctgcgggaa
    59581 ctgcgaacgg atctagcatc agaagcaggc gttcccccgt atgtcgtatt ttcagattcc
    59641 acattgaaag aaatgagccg aatacgtcca tcaagcagat tggagatgct tcagataaaa
    59701 ggagttggcc aaagcaagct tgataaatat ggcgaagcct ttttatctcg gataaaaaat
    59761 gcggactcta acgttctgga taagtagaaa gaacgaatac gtaaaaatga ataaaaacaa
    59821 ctggttaaat ctgttttttt tgctccttat gaaaatcaaa tatgttataa ttaaggaatt
    59881 agaatgggag gtaagcacag atggattctt tgtcgaaaaa acttgcgatc caagtattaa
    59941 atgatgaagc agaacggatc aaacgattaa tccgaaatca aaacaacagt ctctgtatct
    60001 cacaatgcaa agccttcgaa gaagtggtag atacacagat gtacggcttt tcccggcaag
    60061 tcggttttgc tgtccgtata ggagtaatcg gaaagaacga gggccagctg atgttaagtg
    60121 agctagaacg agaattgaat cagttatata gtgaggttta ccaagaaatt tatgataaaa
    60181 aagaagttgg caaggaggaa tagacttgcc taagaaagtg aaaaaaaggc atctattaga
    60241 ctatagcatc ttgattccct atttaatttt atgtgtattg ggattgatca tggtatatag
    60301 ttctacgtcg tatctattat tagaaaacgg acaaaatccg tccgcatcag tcattaacca
    60361 gagtattttt tgggtgctta gcttgattgc tatcgcacta ctttataaaa tgaaaacaga
    60421 tgttttgaaa aaccagcgtt tgatcatggc agccatcgca gttttgacga ttttgctgct
    60481 gattgttgtg ttttttggta aggagatcaa tggggcgaaa ggttggctgc aaattgcagg
    60541 attctcgatc caacctgccg aatacctaaa aatcatttct atctggtacc tgtctctgac
    60601 tttatctaaa agacaaaaca gtgtacaaaa agattttctt gggactgtga aacgtccact
    60661 tgcaatggtt atcggactta ctgcattagt cgctattctt cccgattttg gtaatgcggc
    60721 agttatcttt ttgattattt tggttctgct tttagccagc ggagtcaatt atgtgtatac
    60781 tttgattgta ggagtaggag gcttttgtct aagtactttt acgatctggc tgatcaatat
    60841 cacaaacgga aagatcttac ccggacgttt acagtatatc tataatcgat ttgctattta
    60901 ccaaaatcct ttctcagacg aattaaataa agggcatcaa ctagtcaacg gctactatgc
    60961 catgttcaac ggaggactat ttggcagggg attaggtaac agtatccaga aaaaaggatt
    61021 tttacaagaa gcacaaaccg attttattta tgcaatcgta gtggaagaac tgggagtgat
    61081 tatggggatt ttgatattag cgttgctatt ctttatgatt gtgcgcattg tccttgtagg
    61141 tatccgttca aaagatccgt tcaattcgct tttgtgcatt gggatcggcg caatgttttt
    61201 gatccaagta tttgtcaatc ttggaggaat tactggcgtt attcctttga cagggatcac
    61261 gttcccattt ttaagtcaag gaggctccag tttgctgatg ctttcgattt gtgtcggctt
    61321 tgtcctcaac atcagtgcgg atgaaaaacg caagagctta ggtttataat aaaaaaagat
    61381 gtaggagtgg ttcgatgaaa aaagttttag ttgctaacag gggtgaaatc gctgtacgtg
    61441 tatttcgtgc ttgtacagaa ttaggaatta aaactgttgg aatctatgca gaagaagatg
    61501 aatattccgt acaccgtttc aaagcggatg aagcatattt agtaggacaa ggaaaaaaac
    61561 cgattgatgc gtatctagat attgaaggga tcatttctat cgcgaaagaa tgcggagctg
    61621 acgctattca tccgggttac ggtcttttgt cggaaaactt gaattttgcg caacggtgcg
    61681 aagaagaagg catcattttt gttggaccca aactgcatca tttagatatt tttggagata
    61741 aaatcaaagc aaaagctgca gctatcgaag cggggattgc ttcgattcca ggcacagatg
    61801 gaccaatcgc ttctatagac gatgccttgg agtttgcgaa acaatacggt tatccgatta
    61861 tgatcaaggc agctctaggc ggcggcggac gcggcatgcg tgtagctcac gatgaaaaga
    61921 gtgcaagaga gggctacgaa cgtgcgaaaa gcgaagcaaa agcagccttt ggtagtgatg
    61981 aagtgtatgt tgaaaaatac atcgctaatc ctaagcatat cgaagtacag attctcgggg
    62041 atacgcatgg aaacgtcatc catctttttg aaagagattg ttctgttcag cgccgtcacc
    62101 aaaaagtagt agaagtagcg ccttgtgtct ctatgaatga acagcaacgt caaaaaatct
    62161 gccaggcagc tgtccaattg atgaaatatg tcgggtatgt caacgcaggt acagtagaat
    62221 ttcttgttga aggagatgat ttttacttta tcgaagtcaa ccctcgcgtc caagtggaac
    62281 atacgatcac agaaatgatt acagatattg atatcgtaac aacgcaactg ttgattgctc
    62341 aaggtttgga tctgcataaa gaaatcggat tgcctcagca agaaggaatt aaattaaatg
    62401 gttcagccat tcaatgccga atcactacag aagacccgtt gaataacttt ttaccagaca
    62461 cagggaaaat cgatacgtat cgttcacctg gtggttttgg cgtacgtctg gatgtcggaa
    62521 atgcttacgc gggctatgta gtgacaccgt attttgattc attgctagta aaagtatgta
    62581 cgcatggtgc tacctttgaa acagccattc agaaaatgga acgttgcttg aaagaattta
    62641 gaatccgtgg agtgaaaaca aacattccat ttatgctcaa tgtcatcaca catcctgaat
    62701 tccaatcagg aaatgcaaaa acaaccttca ttgatagtac agcaacatta tttgaatttc
    62761 ctcgactacg agaccgcgga aacaaaacga tgaaatatat cggtgaaatt actgtgaatg
    62821 gttttccagg tatcgaaagt ggcgaaaaac cattttatga agaaccacgt atgcctaaag
    62881 atctaatcac aagaactgat tacgtaacgg ctaagaatgt attagatgcg aagggtgccg
    62941 atgctttggt cgaatggatc aaaggacagg aaaatttgtt gttgacggat acgacattta
    63001 gagacgccca tcaaagtctg ctagcaactc gtgttcggac aaaggatttc aagcagattg
    63061 cacgtttgac tggagaaggt cttcctgaac tcttttcaag tgagatgtgg gggggagcaa
    63121 catttgatgt ggcttaccgc tttttgaatg aagatccttg gcaacggttg agaaaaattc
    63181 gttctttaat gcctaatacg ttgctgcaaa tgcttttcag aggttcaaat gccgttgggt
    63241 attctaatta tcctgacaat gtattggtgg aatttgtcaa agaagcagca gcgcaaggaa
    63301 tcgatgtatt tcgaatcttt gatagtttga actggactcc acaaatggaa aaaagtatcc
    63361 aagctgtacg ggatactggt aaaatcgcag aggcagctat ttgctatact ggcgatatca
    63421 acgatccaag tcgggcaaaa tacaatgttc agtattataa agacatggca aaggaactag
    63481 aacaactggg tgcgcatatc attgcaatca aagatatggc tgggctgctt aaaccgcagg
    63541 cagcttatcg tttgattagt gaattgaaag aaacgacaga tctgccaatc catctgcata
    63601 ctcatgatac cagcgggaat ggaatcatca cttattctgc tgcttcaaaa gcaggagtag
    63661 acatcgttga cgtggcaatg agtgcaatga gtgggaatac tagtcagcct agcatgagca
    63721 gcttgtatta tgcactggtc aatggtccac ggctgccaga aatcacgatt gaaaatgcac
    63781 agaaattaaa ccattattgg gaagatgtgc gcatgtatta caaaccgttt gaaaacggct
    63841 tgaatgctcc tgaaacagaa gtatatatgc atgaaatgcc aggaggacag tattcgaatc
    63901 tacagcaaca ggcaaaagct gtcggattag gtcatcgctg ggatgaaatc aaacaaatgt
    63961 atcatactgt caacttgatg tttggcgata tcgttaaagt aactccatca tctaaagtag
    64021 ttggagatat ggcactattt atggtccaaa atgatcttac agaagaagat atttatgaaa
    64081 aaggcgaaac actaagtttc cctgaatctg ttgtgacttt cttccaagga gaactagggc
    64141 aacccgttgg cggatttcct aaaaaattgc aaaagattat tcttaaaggc agacctgcat
    64201 tgaatgaacg tccaggtctt ttggctgaat cagtcgactt caacgaagta aaaaaagaat
    64261 tggcagaaaa aattggctat gaaccaaaac aagacgaagt attaagctat ttgatgtatc
    64321 cgcaagtctt tttggactat caaaaagcat ataatcaatt tggcgatgtt acattactgg
    64381 acacaccaac atttttccaa ggtatacgtt tgggtgaaac aatcaatgta caaatcgaac
    64441 gcggaaaaac gcttatcatt cgtttagatg aaatcggaga acccgatatc gaagggaatc
    64501 gcgtactgtt cttcaatttg aatggacaaa gacgtgaaat cactatcaaa gataattcga
    64561 tcatcagtac ggtccaaacc cgattgaaag ctgaaccgac aaaccgagaa caaattggag
    64621 caacgatgtc tggttctgtt ttggatgtat tagtgaaaaa aggcgataac gttaaaaaag
    64681 gtgatacgtt gatgattact gaagcgatga aaatggaaac ggccatcgaa gcacgttttg
    64741 acggagaagt cgcacatgta tatgtgtctt caggagacac tatttcgtct ggtgaccttt
    64801 tgattgaagt gactgagaaa taataagcga aaggtgggga aacatgaaaa gactgggttt
    64861 gttttggctg attctgctat tagttattgt ggcattctac atggaaccag tcttgtttcc
    64921 gccaaaactt gaagtgaagg aaaactctga tcaacaaata tcaaaccaaa tgacaacgtg
    64981 gaaataccag gagcttaaag caactggttt ttctacttat atcggaaaac cgatcgatca
    65041 gctggaaaag gattttggcc aagcctatga tcgattaact agcggatttg gttttgaaac
    65101 aagatattat gaaaataaag ctagtcatct cacatttgaa gcgaacatcc aagacagcag
    65161 agtcacaact gttaaagtac tgcaaacagg gcctgctgat atcgcgccgt tttatttagg
    65221 gatgacgatg caagatctta cgaaaatcac tactatctat actaatttca cttttgagta
    65281 ccatgataca gaagttggaa tcgaactaat ggaagaggat atgaattatc gtccgctaat
    65341 tgcttttgat aacgacactt ttgctatttt attttttgat caaacaacaa acggcctatt
    65401 ttcagttgtg tatttagata aagatagtct tttgaaacta ttgccatatc aagtgtttgg
    65461 tgaaggcctt ccacattatc aagcagataa atcggctgat tgggaatcaa tcaatcgcgt
    65521 taaagaacga aaaagtacga ctttattaaa tatgcttaga agagaagacg aacttcccat
    65581 atacaatcaa acacttgcct ttcaaaacca cagtcagctg ctactacatg attttttgac
    65641 aaggcctgag gaaatattgt cagaggaacg tatggctgat tggcaaaaat cgctaggcag
    65701 tgctcaaacg acagtgaaat tctcactgac gaatgatgaa ttggagaagt taatcagtaa
    65761 acaaaaatta gaaaatacta gtgggctatt tattcatccg gcgattgacg ctactttttc
    65821 gtttttatat tggtacagcg atccttacaa ccatgaccgt ttcatggacc cgggaacgga
    65881 ttctgtaggc attgcttttt ctaaagaaaa tatgttagtc ttattgcaag aagaaaacgg
    65941 agaaagtagt gatagcagtg atcgttaacg aagagctgtt tcaactggag gatcaatgcc
    66001 tccaagtcgc tgaaatgata aaaaagagta aaacgatggc ttcttatctt gaacataaac
    66061 agtcaatgga agaatctgca aaagtttgcc agttgaaaga agcgttcttg tctaagaaag
    66121 aagcatttga aaatgtagcg gcatatggtg agtatgcacc tgattatcgt gaaaaaaaac
    66181 gagctgttcg aaaagcaaaa agacagcttg atatggatga gcatgttgca gctttccgat
    66241 atagtgaaac acagctgcaa agtctgttgg atcaaattgt ccaagtactt gccgaagaag
    66301 tttctaatga aatcaaagta gatgcaggta gcccgttttt tgaaacgaaa aaacactttg
    66361 gatgcggagg taattgtcat gcaattagac aatagtgaat ttgaaataac aaaacgacgc
    66421 gggctgatcg tatgggtcta cagcttaaaa caattaaaga atctgaaaaa gttcggcttg
    66481 ttgcattatg tctcaagaag aatgaaatat gtcgtgcttt acttaaatga agagacattg
    66541 gaagaaacag aagaacgaat ccgtaaatta cattttgttc gaaacgtaga acgttcttac
    66601 cgaccggata tcgaaatgaa ttttgccgaa aaaataggaa acaaaacagt attaaaagac
    66661 gaaggtttcg aaattgaaga gctaaataca gaaatccgat tagctgaaca tctttaaaaa
    66721 tctcttatga gcagttttta aataaaaaat aacttggata acgatcgatt tttgatcatt
    66781 gtccaagtat ttttttggta tactagtacc agtaccattt ctaaggagtg agtagatgcg
    66841 gataatatca ggaaattatg gcgggcgccg tttaaaaagt ttagcagggg cgaacaccag
    66901 accaactaca gataaagtaa aagaatccat attcaatatg attggaccat attttgatgg
    66961 agagacagta ttggatctgt ttgcaggaag cggtggttta gctatcgaag cagtatctag
    67021 aggctgcagt catgcggtat gcgtagacaa aaattaccaa gcgctaaaaa tcatcaaaga
    67081 aaacatcgaa attacgaaag aaccagagaa atttaccaca ataaaacttg atgcggacaa
    67141 agcaatcgaa cggctagcag cggagaaaca acaattcgac tacttgtttc ttgatccgcc
    67201 atatgcaaaa caaaaaataa tcgatcaaat ccataaaatg gaagaactgg gacttttttc
    67261 taaaaccgca gttatcgtat gcgaaacgga taaaacagtc gaattgccag aaactgtggc
    67321 tggattccat caaataagaa aacagattta tggcataacg gccgtaacta tctatagaaa
    67381 agagggaaat caatgaaacg tgcattattc ccaggaagtt ttgatccttt tacaaaaggt
    67441 cacttagata cggtggaacg tgcggctaaa ttattcgatg aggtagtaat tggtgttttt
    67501 atcaatactt caaagaaaag tttgtttccc ccagaagaaa gaatgacatt gatcacaaaa
    67561 gctgtttctc atcttcctaa tgtaaaagtc atgcatcaag aaaatcaatt gacggtagaa
    67621 acagcaaaag agattggtgc agatgcgttg atcagaggca tacgaagtat caaagacttt
    67681 gaatatgaac gagagattgc tcagatgaat catcatctat atgatgaatt ggaaactgtc
    67741 ttcttattag caaaaccaga atatagtcac gtgagttcca gtattttaaa agaagtactg
    67801 cactttggcg gagatgtgag cagctactta ccgccagtga tcaacgaagc tttagctgga
    67861 aagcgtgaat caaatgaaag ctaaacaatg gttgaaaaca gtactgatta tcatatttgg
    67921 aatgttgatc attggatttg tagtagttcc cttgccgtat tatgtagaat caccgggagc
    67981 cacgatcgat ctgaaagaac taattacggt caatgataag gaagataaag gttctgggtc
    68041 tttctctctt acctctgttg gtatcaggca agctacaggt ttcttagctt taaaagctaa
    68101 aatgacccct tttgaagaat tgatcagtga agaagaattg actggtgggg cgacaaatga
    68161 agaatataat caaatgcaac agtattatat ggattcttcc caaaatgcag caatcgaaca
    68221 agcgttaaaa cttgcagaca agccttataa aatggaattc aaaggcgtat atgtcatgag
    68281 tgtagagaaa acatcgaatt tctttggcaa gctttcagtt ggagatacag taacaaaagt
    68341 agacgggaaa agctttcaaa gtacagaagc atttatggat tatgtaaaaa gccaaaaagt
    68401 tggacaaaca atcgaaatca gttatttgca taatggtgaa gaaaaaacag cttctggtga
    68461 tctgatcgag ttaccaacag ataaaaaggc aggaataggt atcacgctta ctgaccatac
    68521 cgagatcgaa tcagatacag atgttcgtat taattctggc tctatcggtg ggccatcagc
    68581 gggattgatg ttcactttag aaatctatga acaagtcact ggtaaaaact tgcgacatgg
    68641 taagcaaatt gcaggaactg gaacgatcaa cagtgaagga gaagttggcc gaatcggagg
    68701 aatcgataaa aaagtggcaa gtgcggataa agctggcgta gagattttct ttgcaccaga
    68761 tgatacgatt tcagacgatg ttaagaaaga atatcctgaa ataaaaacca actacgaaga
    68821 agcaaaagct gccgctaaaa aattgaattc ctcaatgaaa atcgtaccag taaaaacagt
    68881 acaagaagcg cttgactatc tagagaaaat gaaatgaaac taaataatta aataggggtt
    68941 gtgacacgag ttctgtcaca acccctcttt ttcgtcaaaa atcttcacga taaaaataaa
    69001 aacaaaagaa aaaattgacg taattagatg ttgaagggac gattttgcca gagctcactg
    69061 tttcttcaat tttaaatgaa aggagtaatg aatgattttt actggtcatt ctttgaaaaa
    69121 agaacaatga tagaaaaaat cagcaagaaa atactagctt cacccaaatt gatcatttgg
    69181 atgtctatga tagttcttat cttttgtatc gcaagtggtt ttttattatc aaatttgcta
    69241 ttttatagag aaacagaaga gactgctgca aaagaaatac tttcttcgtt tagttccagt
    69301 ggagaagatc catcagctga aggaaacgat gtagaagagg atgctcgtcc aaaggtcatg
    69361 tacaccgata tcaaaggaag tgtgaaggag cctggtatct attccttttc ttcagaagaa
    69421 cgggtctatg atgtactaaa gcgtgcaggt ggattattag aagaagcgga tagcgatcga
    69481 atcaactttt ctgcgaagat agaagatcag caagtcctat atatccctgc tgtaggtgaa
    69541 gagctgccag aacatcttga tcagttggca tcctcggaag gaaaacagcc tacagcagat
    69601 tccgagcctc ccaaaataaa tatcaataca gccactccat cagaacttca gcagattcct
    69661 gggatcggat cagtcaaagc gcaagaaatc attcgattta gagaagaaaa cggatccttc
    69721 cagaaagtgg aagacctgca ggagatttct ggtatcggtg aaaagacagt tgaaaaacta
    69781 aaaaacttcg ttacaattaa atagaatgat tcaaaatcga ctggcacgag caataaatgg
    69841 acgctaatgg atactaagaa taatccaatt ctaagagctt ggcaggacga ggaggtagta
    69901 tttttggata ggaaatagct tgattgttcg caaagaaaca ttaaaaaaac caatgctgaa
    69961 tgtctatctc caaaataaaa tatctgggat tcatattatg aatacggctg tcagcggcaa
    70021 taacagtcaa gctctacgag aacgctttgc caaagatgta cttagctata cagcagataa
    70081 agtatttatt ctcattggga cgaatgatct agcagagcat aaacaacttt ctaaagaaac
    70141 gtaccaaaaa atctgcagtg gatgatagtt cagctgaaaa aagactatag ttctgatcat
    70201 gctttcttta tcacgccacc agcagtcgat gagacaaaac aaaaatatcg gaataacaag
    70261 ttgatcaaag aatatatctc tgttctcaca gaagtagcaa aacgccatga ttgtgaagta
    70321 tcgattttta tacggaattt cttgaacaaa tggattcgaa gaatttttat aatgggctaa
    70381 tggatgacgg acttcatttc ggcagtgaag ggtaggatct tttgtcagac aggatgattc
    70441 gttatattta aagaatattt tgtttaagca atgagtactt tttaatcaac ttgaaaaaac
    70501 ggaggaatat aaaacatgtt agaaaaatct tcaaaagcat gcgatacgaa taaagaaaaa
    70561 gttactgata agtcacataa tgaacgcatt ccttgggacc agtactttat ggctcaggca
    70621 gtactgttat ctttacgtag tacgtgtaca cgtttagaag taggagcgac actcgttcga
    70681 gaaaaaagga tcattgctgg cggctataat ggagcagtca gtggagatgt ccactgcata
    70741 gatgaagggt gttacgtcgt ggatggacac tgcttgcgaa cgatccatgc agagatgaat
    70801 gcactcctac agtgtgcaaa acttggtatc ccgacggaag gttcagaaat ctatgtcaca
    70861 cattttccat gtttggcctg tacaaaggct ttgttgcaag ctggtgtgaa agggatcaat
    70921 tatctccatg actatcgaaa tgatccttat gcccaagcgt tgatcgaaca attaggtgta
    70981 tctgtacacc aagttgcttt agactcacag catttttcaa aacttcaaaa tgaattaaca
    71041 ggaaaacatg tctagcgttt tagaaaggca tgttcgaaat cggttgattt ttccggcaat
    71101 tacgtcgatg attatctgtt tgttgtatta cactgacaat tggggttatc gtttgcttct
    71161 tgccggaatt tttttatggt tttcgctaaa agaaggacgt tttatcgcgt acttgtgtat
    71221 tttgtgtagc ataacggtat ccatttctta taggctacaa gagtattcat ttttagagga
    71281 aaaaacaaca tctgcgcaaa ttactgtatt tacagatacg attcgtgcaa atggtaattt
    71341 tttgacaatg gaaggaaggt tatcagagaa taaacaaaag ctccagcttt cttatcaagt
    71401 aaacacagaa gcagaattga aaacatggct taacttccga ggaaagggaa tagaattgac
    71461 tgctgaaggc gagttcatct ctccagcaaa aaagcggaat ctttataatt ttgaccaaga
    71521 agcttacttc aggaaccata gaatttctgg acggttccaa attcaaaaga ttgaacagat
    71581 caccatgcaa aatcactggc gaaactggct tcagagaaaa agaggatctt ttatctcctt
    71641 tgtccaatct gaatttccgc aaaaaacaag tgtatatatt aattctttat tatggggcta
    71701 taaagatgca ggattcagag aaccagaaga aatttttagg gagagtggtt tgctgcatct
    71761 gttcagtcta tcgggtctac acatccaatt ttatttaggg tggctgtatt atttgtttcg
    71821 acgtatcgga tggccactac acgtcagtat tattccgttg agtctactaa ctatctgtta
    71881 tgtttctatt gcaggaagtt ctatttctgt tttgcgtgct agtttgctgt ttttattgcg
    71941 attgctagtc aaattgctga ataagcatta ttccaccatg gacttatttg cagttgtctt
    72001 ttggcttctt ttactatttg atcctcgatt tctttttcat gcgggtggac agcttagttt
    72061 tttcatgagt tttttgttta tcctgatttc ttttgatgga gattggttga aaaaaacggc
    72121 ctctcacgtc ctgtttacta tgatcacgat gccgttgatt gtatggcatt tttatgagtg
    72181 gcctatttta ggtagtcttt ttacattact gattgcaccg gtattcaagt ttctttttct
    72241 tcctggtgtg ttatttctca cactcttcaa tcgctggctg ccggaaactt tacttttgct
    72301 gatagaagag ggattaatct tatttgagcg aataatcgat ttttcttcag ggtcgtctct
    72361 cattatagga cgtttgcccc ttgtgctatc tgggatgtat attttcggat tactgatttg
    72421 tatggatagg ttgaaagaac aacctgtgaa acacttgttt tatgttggtc ttttttcttt
    72481 tatttttttg actttgccat ttttacgctt ttcttcaacg atcgctttta tcgatgtcgg
    72541 tcaaggagac agtatctttt tgcaaagtcc ttttcgaaaa gaaacgattt tgatcgatac
    72601 tggaggtcag ttacactttg aaagagaaaa gtgggcgcaa ggtatgatac gtcctcctgc
    72661 tgaaagaaat atcgttccat atctaaaagg tcaggggata agtgtaatcg ataaactcat
    72721 cttgacacac gacgatacgg atcatgtggg agaattaccc acgttatctc agcattttac
    72781 tatcaaaaaa atttatatcg gttggggcgc tggacgaaat gagccattac aaaggcattt
    72841 atctgaagct caaaaaaatg gtactgagat cattgaaatc aaacatgggg acaggatcag
    72901 cggttattat gatctttatg tattgactcc ttttgaaaag ggagagggaa gaaatgaaga
    72961 ttcgattggt ctatggatgg aatataacaa taggcgcttt ttatttttag gagatttgaa
    73021 tcaagcaatg gaaaaacaat tgcttttgat ttatcctaat ctgaaagccg atgtagtcaa
    73081 attaggtcat cacggtagcc gtacgtcaag taacacagat tttctttccc atattgaagc
    73141 aaaacatggg attatatcct gcggcgtaaa taatcgttat ggtcacccgc acagcgaagt
    73201 attggagaca ctagaagaaa atcagataaa aacatggcga acggatcaac aaggaatgat
    73261 cacctatcgc tggcatccag tttttcatcc tcatgggcta gtggaaacaa tgatagacta
    73321 gtaattctca tctgctcatg gtaggataat ttttgaaagt ggtgagtaat ttgagtatac
    73381 aagcagagtt acaaaaaata cgtaaagaaa aacttgcatc ttgttacttg attttaggaa
    73441 cagaaaagtt tctccaagat caagttcgga cagaaattgt caaaaaaatc caattagagg
    73501 gcgaagatga cttaaatttt ttaagttttg acatggaaaa tgcaagttta gatgaggtag
    73561 tagctgaagc agagacgttg ccgtttttcg gagatcaacg gttagtattc gtagagaatc
    73621 cttatttttt aacaggtgaa aaagtaaaca atggaatcga acaaaatact gatttattga
    73681 cggcttattt gaaagaacca ttagaatcga ctgtattagt attctttgcg ccatatgaaa
    73741 agttggatga acggaaaaaa gtaacgaaac aattgaaaaa aacagcaatt atcatcaatg
    73801 tccaacagtt gaatgaaaaa gaagtacgtc aatatctgat gaatacctta gagaattcct
    73861 caatcaaaat ggaacgaagt gcaattgatt tattcttgag gttgaccgac ttagatttat
    73921 ccaaaatgat gagagagtta cagaaattgg tcctttatgg acaaaatcaa cagcaaatca
    73981 cggtaagaga agtagaagaa ttagttccta aaacgttaga acataatatt tttgacatga
    74041 cccaatatat tttaaaaggg aagacggaac aagctttgcg tttatacgaa gatttagtta
    74101 tgcaagggga agaaacaatc aaaattaatg cgattttgct ttctcaatta cgtttatttt
    74161 tacagacaaa atttcttgta aagattggtt atcagcaggc aaatattgct gaaacattga
    74221 aaatccatcc atatcgtgta aaacttgcca tgcaggaagt aaaaaaattc gatgaacatt
    74281 tgctggtaca gttgtttgat caattagttg agatggatta tcaaatcaaa acgggacaaa
    74341 tcgaaaaaga attaagtttt caactattcg ttttgcgcac agggcagttg ttaagaaatc
    74401 gataagaaga aagacgaggg caaagcatgc gggaaggtcg tttgtttttg tccttttttt
    74461 gacaaataaa aaaatcggcc gaagccgatt cttttattta gcgatttttt tgcttaaacg
    74521 agatttgtcg cggttagctt tgtttttgtg gattagacct tttgattcag ccatatcaat
    74581 agctttaaca gcttctttat ataacgcatc tacgttgtca gcacctgaag ctacagcatc
    74641 ttcaaatttt ttgattgcag tacgcatagc gcttgtttga gatgaatttt tcacattagc
    74701 gttttcgctt gtacgaacac gtttgattgc agattcaatg tttggcatta gtttcacctc
    74761 cgataactta agttcttacg cgtgttaccg cataattcaa cattcattat tatacctaaa
    74821 ggcagcgtgc tttgcaatat gaacatgaca agtttttttc cttaacagtc ttttattagg
    74881 attttttttc agtaaaaacg gatagtacaa gctgaaaaaa atcaaaaaga cgctattgtg
    74941 ggaggcaata gcgtcaaacc aaactagatt tttatttatc tatttaaaca aagtttgaat
    75001 gggaatgttt ggtactaagt tattatataa caaagagaac cgtttgcaaa gttttttttg
    75061 aaaagggata cgattccctg ttgttctttg tttttctatt aagaaactgc agttatttta
    75121 taaccagtat cctttgatca gtaaaggcta ttctggtgta ccgattattt taatgtattc
    75181 atcgatattt tttatcaaca ttttttttat gaccaactgg ttccgacggt tccatttgcc
    75241 acttacttcc agcacatatt tatttagggg caaacgtaag atatattttg ccaaatagcg
    75301 atctttaagc aggccattga tcacacggtc ctcaagctgc aagttgatcc cagtcagcat
    75361 cattggctgt tcttgtaaaa cgtacgtatc aattaaaaga ccttcaaaag tttccacaag
    75421 cgtcttcctt tcttttcaaa gtaacctgaa aacaggcact aattcttcca ttttgatatt
    75481 cgttgaagtg gatgcgatca ttctttcttt tgtccactgt aatctagtat aacaaaaaag
    75541 agcagaagag ccaaaaagaa aaaagagcat cgaattctat aggaagtctc attggcacgg
    75601 cctaaattat tacaaaacat tgattaaaaa aagcccctgc atcgcaggag catcccctat
    75661 atcgttttaa taggtcaatc aaaaagcagt atcaaacaca ttttgtatca aatcaggaaa
    75721 taatagataa gctaagccca gaatcaaaag taccgctaga agaaaccagc cgattgttcc
    75781 tatgaaatac tttaattttt cgaacattgt atcacctcgt tgtcagtata tcacggatca
    75841 gtaaattggg ggtgttaaga atctcctaaa atagacaaaa tttgttctat tttcctaaag
    75901 caaatacttg taagatacag taagtgatac cagaagcaat gaccggtccg gcggcgattc
    75961 ctcttaagaa gacaacacct aaaatcgtgc cgaaaacaag tgcaacagtg atttgtggat
    76021 cagaagcaat caatcctaca cccttggaag ataagacagc cactagccca ccgcaaaaaa
    76081 cagctaccca gccaataggg gatttaaagg cttctaatag atcacgtaga ccgatttctc
    76141 ctgttgcgat agggattaga atagccgctg tgatgatcgt caccccaata ttgattcctt
    76201 tgccttgaag ccaagggaaa aaggaacggg aggctggtat gagcttcata atcaaaacaa
    76261 aagctgccgc tatcatcaga ctttgatttt tagctaaaat tgctacaagt aaaataattc
    76321 ctaaaaaaag ccaactttcc atgaaatata ttcctcactt tcttcgaaac aatcataaca
    76381 tgtttttaga aagttacaaa agtaggaatt catccttcaa gacctattca taagctgttt
    76441 ttttcataaa ataaacaaaa tcataaaaaa acactttgct tatttgacag aagacagccg
    76501 cgctttgtat agtggacaag acttacattt ttttgaagaa tcagaaagat caggtaaagg
    76561 tcttagtaaa cgcaagccat tcagaataac caatattgtg cttccttcgt gtcctacaac
    76621 tccaagaggt agatttaaaa attgcaggaa gttagaagca atcaacaaga gaataacaag
    76681 aatcgaaaaa ataatatttt gtttgatgat ccgccgcatt tttaaagata gccgatgact
    76741 gtagacaaat ttatttaagt cgcttttcat taagacgatg tctgcgatat ccatagcggc
    76801 atctgtccct tgccccatag cgacacctaa agaagcggct gctaaagcag gcgcatcatt
    76861 gactccatca ccaaccatcg catttacttg gtaaagttca ttctcttttt tgactagttg
    76921 tgttttttct tccggagtac agcttgcata gtaatgttca atccctactt gttcagcaat
    76981 ggcctttgct gtgccagaat ggtcgccggt tatcatactt gtatggatgt tttgggattt
    77041 aaagtaggaa attacttgtt gggtatttgc ttttggaata tctagcagac ctaaaacagc
    77101 aattaactgc tgatctttag aaagataaat cacggttttg ccttgatttt ccaaacgttc
    77161 gatcatttcc gcaatctctt tgctgattat catcgtctca ggatcatatg cgtgcttgcc
    77221 aactttccat tgcgcaccta aataggtagt ttgtaatcca aaaccagtaa tttcttctat
    77281 tggtaagtat tggatttctt caggtattgt gcaatcgaaa tattgcaaaa tcgcatgagc
    77341 aagtggatgc gtcgtttttt gttccatagc aacaagaagt tgttgtgcca aatccttttc
    77401 atcaaagaaa aagctgtcag ttacaactgg ttttccttta gtgagcgttc ctgttttatc
    77461 aaaggcaatc gcttttaatt cagaaagctt ttctaaatgg ataccgcctt tgattaaaat
    77521 cccatttttt gcagaatttg aaagagcagc tagagaagct ggtgtagcag atgcgacaag
    77581 cgcacaagga gaagccacaa ctaataatac cattcctcga taaaagcttt catcccaact
    77641 ccaacctagg aaaaagtggg ggagaaggat cattagagga acagcaagca aaatcaattt
    77701 tacataatta ttttcgattc gttcgataaa tgaagctgtt tgagttggcg tattctgcgc
    77761 ttcttctact aattggataa tcttagcaaa gacagtttcg gaactcgttt ttgttactcg
    77821 aatcgtgatg ggatttcctt gattcagcgt tcctgcatac atctcatcgc ctaaatattt
    77881 ttctacaggc acagattcac cgcttaatgc agactcatcg atcgttgcct gtgtgctttc
    77941 taaaaagcca tctatcggaa cagctgctcc tttagcgaca aataccaagt tatccacctt
    78001 tagttgagaa acatctactt cttgcatttg tccattttct tttaataatt gggctttttg
    78061 tggttgaaga ttcatcaaag cagtaatttc tttttgactt ttattagttg tgtattcctc
    78121 taatgcaccg cttaaacaaa agatgaatgt caacattgct ccttcaaagt aattaccgat
    78181 caaacaggca ccaatagcag ctaaagccat caataaatcc acattcagtg tccgatcatt
    78241 ccacaattct ttcaagcctt ccgatgtttg tttatatcca ccgatgataa tggcactgat
    78301 ataaaaaagt ggggtgatgg taagagagaa tcggtcgaag ataaaaccga agatcataaa
    78361 taaaaggcag ccgattgttg ctgcaaaagc ttttttctga ttagtcatcg ttattagacc
    78421 tccttgtcaa aacaaggata gcataataaa aatgtaattg ataataataa cagctaaatg
    78481 agaacgataa tggttatcat ttggtgatta agcttgatgt tgtttttatt atttataatg
    78541 attattatta aagaaaataa aaaaccagaa catttctcaa ataagagaag tgttctggtt
    78601 ttttctacat tgcaataaac caataaccaa tcaaaatgat accaaggata atacgatacc
    78661 agccaaaagc ggtaaagtcg tttttcttca aatagcttag taagaatttg atagctaaga
    78721 cggaaaccac aaaagatacg aatgttccta caagaagaac aacagtttct tccattttga
    78781 atgaattccc ttcagctaaa tatttgacga tcttcaagcc gcttgcgcca aacatgacag
    78841 gaatccctaa gaaaaatgaa aactctgtag cgacaaaacg cgaagcacca atcaagatag
    78901 atcctaggat cgttgcgcca gaacgggatg ttccgggaac aagtgccaag acttggaata
    78961 acccaacaag aagagctgct tggtacgtaa agtcattcag agaagtccaa cgaggtgttt
    79021 tgtttttgtt gcgtttttcg acaataataa aggcaatacc gtaaacgatc aaaacaatgg
    79081 caactggtaa gaacttgtgg aaatttgctt ctaaccaatc atccaaaggc aaaccaatga
    79141 tgatggctgg tatgcaagca acgatcactt taaaccaaag tgtccacgta tctttttttt
    79201 ctaccgttga tttacttgga gaaaatgggt ttaacttgtg aaaataaaga acgacgacag
    79261 ccaagatcgc ccctaattgg ataacgacat tgaacatcga cataaattgt gaactggcat
    79321 ccagtttgat gaattcatct gctaggatca aatgaccagt actactgatg ggcagccatt
    79381 ccgttattcc ttcaataata ccgagaatca ctgctttgat aatatttgca aaaaacataa
    79441 attccttcct tcccaattaa gatacatctt atagtatacc gttatttttt gagaaaccca
    79501 atctgttttt taatattaag aaaaacttga gaaataaagc agataagtac acgaagagaa
    79561 aaaagaagac aaagccctcg ttcagacttt gccttttatg attctctggt aaaaattaaa
    79621 atatccccat tatagaaacg agtcgcgact atgtgttttc ccgttatcga tataagacgt
    79681 atcattgatg ccgtggatag aaaatcgacc gtttgcataa gtgacctttg ttacgctgcc
    79741 atttaaaagc ccagcacgaa caggctgttt aggatcgatc agattaagca aaaaggcgat
    79801 cgtcaaacca tgagatacga ctaaaacatt tccaccacca tttttttcgc agtgatgggc
    79861 gatatcttca aatcctgtcc atacacggtc tcgcaatgtt tcataagttt ccgcccaccc
    79921 agcggtatct aagttgtata cagcattagc aatttgttca aaagaaactt ctgtggtaaa
    79981 catttcatca tatgttttaa aattcaatac acgagggatc acaccccaca tttccatgtc
    80041 atatccacct tcaagagacc cgaaacacca ttctctaatc cttttttcaa taaaataagg
    80101 aatttctttt cccttagaat gttctcctag aataatacgg gcagtttcga ttgcacgacc
    80161 actgtcacta gaataagcaa atgaaaagtc gacatttttt aacccgcgac ctaaataatg
    80221 aatgccttct tgtccttgct ttgtgagggg tgtatcacac cagccttgta ctcgttcaat
    80281 tgtattgaac atcgtttttc cgtgtcgtac gatataaagt gtagtttctg tcattttatt
    80341 ctcctcctaa gtcgttttaa cgattgaaaa aaggatttgt ccttttttcc tgttcaatcg
    80401 tcgttgcatc gccatgtcct ggatagactg caaattcatc aggtaataca aacagctggg
    80461 tccgaatact atgaagtaat tgttctaaat tccctgtata cagatcggtt ctaccgatac
    80521 ttcctctaaa cagggcgtcg ccagaaacga caaaatcatc aaaaataaag ctgacgcttc
    80581 ctatagaatg ccctggggta gggacgacgc gaaaagacat gtttcctaaa gtataagttt
    80641 tcatttcaaa ttcatattct gcaggcgaga caataacatc agcaatatca tcatgtctac
    80701 cgagtccaga aagattcaaa atcggatcac ctagccattt ttgctctagt gggctgacat
    80761 aaactgggat ttgataatac gcgcgtaaag gttccactgc accgatatga tcgtagtgag
    80821 tatgggtaag caaaatcgct accggctgtt ggtttgtttt tttgatttct tgtttgattc
    80881 gttccgcatc ttctccaggg tcaatgatca aaagtgcttc ttcgttgtag accaaataac
    80941 agttttcttc aatcattcct gtttttaatc tttcgatatg catgtgcatt cctccttgat
    81001 agccaaaact tctttcatcc attagtataa cgcattcatt gctaggaaac aaaaacaggt
    81061 acttatttct ctttacttat ctgaattatc agactatatg aaaagtattt ggatattcta
    81121 aagagaagga aagtaagttt tcatagcatt ttaatatgtc cttgttacat tagattcatc
    81181 caaaaatcca aattctatat cctatatccc tgcctaatca gcagggtgtt tttttatgtt
    81241 tggaaagaga gctctttttg ctaaactagg ttttttcagg taatcttaga gagaaagagg
    81301 tggtggaaat gatcaaacag tcaaaaatat ctgtgcggca cttagtagaa tttattttgc
    81361 gacgtggaag tatcgacaat cgtaaaaaaa gtaaccatac tgcattagaa ggagcgagaa
    81421 tccaccgcaa gctccaaaaa gatgcgggag aaaactatga aaaagaagtc tttttaaaaa
    81481 caacagttca attagatgaa tattacctga tcgttgaagg ccgtgcagac ggtattttca
    81541 aaagagacgg tatttattat atagatgaaa tcaaaacctc cgaaccacga tttgaagatt
    81601 tagaaccaga acaagtggaa cttttctttc atcaagcaag ggtgtatgcc tatatctata
    81661 gtcaagaaca tgatttacaa gaaataaatc tgcaattgac ttacttccag acggcagaag
    81721 aagtgatcac gagaaaggtt gaacatcaga caagagagca gttagaaact ttttttaaaa
    81781 agctgactga agattataaa gaatggcttg tttttcaaga aaactggcgc actgtacgca
    81841 atgcctcttt gatggctttg aaattccccc atgatgagta taggaaaggt cagcgtgaac
    81901 tagcggtagc tgcttataaa acgatccgta cgaaacagaa attgtttgta gaagcaccta
    81961 ccggaacagg aaaaacaatc tctacgttat tccccgcttt gaaagctgta ggggaagaag
    82021 agggagaaaa gatcttttat ctaacggcta aaacaataac tagacaagta gctgaagatg
    82081 ctatgacagc attaaaagat acaggagctg aagtaaaaag tgtcactttg acggcgaaag
    82141 acaagatctg ctttttagat gagacgacct gtaatccaga tcagtgccca tatgccaatg
    82201 gttattacaa tcggattaat gaaggtttat gggatctttt gaatcatgag aatcagatca
    82261 ctcgtgaagt catcgaaact tatgcaagaa agcatacact ttgtcctttc gaactatcat
    82321 tagatgtcag tatctggtgt gacgtaatca ttggggatta caattattta tttgatccaa
    82381 ctgtttattt aagacgtttt tttgaagatg aaaaaaacga ggactatctg tttttgattg
    82441 acgaagcaca taatttggtg aatcgttccc gagagatgta ttcggcagaa ctatcttatg
    82501 aaaaaacgaa acgaagtaag gaagctatac caaaggaatt caaaaaactt catcgccgtt
    82561 tcaacaaact attaaaagaa tttgattcga tccgagaaat agcaaaagaa gatcattggg
    82621 attatcatca tcaaaaagca ccggctgaat ctttagtaaa ggcaggttat caattaagcg
    82681 aaaaaattaa agaatggctg gcagaatttc ctgagcaccc atcacaagaa caattgcttc
    82741 cttattattt tgatttgctt cattttttga aggtcagtga attttatgat gatcactacg
    82801 aaacaacagt cgaaaaaacg tatcgcgatc tgatagtaaa agagttttgt attgatccaa
    82861 gcttgtttct tgagcagtcg ttggataaag gaaaaagcag tattcttttt tctgctagtt
    82921 tttcaccact ctcttattat caggaaacac tggggggaca aaaaagtctg gcttacaaac
    82981 tgccgagtcc tttcccagaa ggaaaccagc aagttttcat cgcaaattac ctagagacga
    83041 cttatcgaaa aagggaacac agtctggcaa aattagtaga gacgattcat gcttttgctg
    83101 atggaaaggt cgggaattat ttcgttttct ttccttctta tcaatattta gatcttgcgg
    83161 tggaagcatt cagacagcaa tatcccgaca gcaatgtgct aatccaagaa acggacatga
    83221 atgaggaaga acgcgaacga tttttaacaa aatttcaaac aaatcccgct gaaacattga
    83281 ttggattttg cgtacttggc gggatttttt cagaaggaat cgatttgaga ggggaccgat
    83341 tgatcggcag tatgatcgta ggtgtaggtt tgccgcagat gaatcatgag caggaactga
    83401 tcaaatcata ttatgatgag aaggaacacc ttggatttgc ttacgcttat cagcttcctg
    83461 gaatgaacaa agtcttgcaa gctggtggtc gagtgattcg tggcatggcg gatcaaggaa
    83521 tcgttgtatt ggcagatcaa cgattttcta gtttttctta tcgacgttta ttcccacaac
    83581 attggcaaac agcaagagaa gtacgaagtg ttggggaatt ggaagaagga atacagcgtt
    83641 tttggttatc aaaagaagca gtaaatcaaa aaaacgagga atgacagacg tcagcctcgt
    83701 tttttgatta acgatagtta gtgaattgca actcgattgg taagtcaagt ttacgtaata
    83761 aagcaatcac agcttgtaga tcatcacggt tcttacctgt cactctaatc tgatcttctt
    83821 ggatttgtgc ttttactttc agtttttcat ttttgatagc tgtagtgatt ttcttcgcgt
    83881 tttcacgatc gattccgttg accaaatcgg cagtttgtcg agctttgcca cctagagcgt
    83941 gttctgattc ggaaaaatgg atatttttga ttggcacatt tcttttgatc agcttaccaa
    84001 ataatacatc tttgatctgt tcgattttga aatcatcatc gctcaataca actagattgc
    84061 tgttctctaa tttgatttct actgtagaat ccttgaagtc aaagcgattg gacaattctt
    84121 ttgttgtttg ctgaatcgca ttttttactt cttctgtatt gacttctgat acaatgtcga
    84181 agctagcatc ttttgccata ggaaccctcc tgatatacat ataaatattt accatacatc
    84241 tatcttatca taggattagg gcagattctt acctgtttgc taaaattctt ctcatttgag
    84301 ttcgaagtat tttattttaa tagataaact gttattctta catttggaat accaaagtag
    84361 gggagaggtg cttgtgagta aaattatgac tgcgtatcat aaaaatgaaa gattaaagaa
    84421 aatagtggtt attttgataa ccggattgac cgctgcggtc gctttaaatt attttttgat
    84481 tccagccaat gtcttttctg cagggatgaa tgggatcgct caaatcattt cctcgctttt
    84541 atatgaatgg tttcatatca agatagacac aggaacattt atttttcttt tgaatatccc
    84601 tgtgtttgta ttagggtttc tcaaagtagg aaaacgagca acgatcctta gttttgtgaa
    84661 tgttgtatgt gtatcggtga tgacgatgct gcttccagtc ggacagatca ctgataatat
    84721 tttgatgaat gccctagtag gcggtgtttt attaggtctt ggcgtaggat tatctttaaa
    84781 aatgggcttt acaactggtg ggatggatat catctctctt gtactttcac aaacaactgg
    84841 aaaaacagtc ggaaactaca tgctgattct aaatggtatc attgtagtca tcgcaggctt
    84901 cttctttaac tgggaaagtg ccttgtatac gattatttcc atttatgcaa tgactcaagt
    84961 aattgatgcc atccacacga gtcatcaaaa agtaacagca atgatcgtta ccgtcaagcc
    85021 agaagaggta acagaagaac tagcaaaacg gatggtacgt ggaatgacat tactgccgtc
    85081 tgtcggtggc tattcaaaaa aacaagggaa catgatcatg atggtcatca cccgctacga
    85141 aatgtatgat cttgatcaga tcgttcatga aatcgacgac gatgcattta tcaatatttt
    85201 accaactcat tcagtatttg gccggtttgc aaatgaaaac gaacaaaaaa tgtatcgaac
    85261 aactggcgta ttgccagaaa taaaaaatcg ttcacaaaaa aaatgaggaa tcaggtttat
    85321 tttagaataa atttaatcta ttacatctaa taagaacatt tttataaatt taaacgagaa
    85381 tcttacttct aaattctcat ttacaagcag ctaaaaaaag agtatgataa ataaaaatcg
    85441 tgttaaggag tgtcgtacga tgagcgaagc aaaccaagaa cggtctggac aagaaataga
    85501 actaatcaaa gaacgtattc ttgctgcctt ggaaatggtg attgatcctg aattaggaat
    85561 cgacatcgtc aatttaggct tgatttatga tatagaattc aatcctgaaa atggagaaac
    85621 agtcattaag atgactctaa caactatggg ctgtccatta gcagatattt taacagaatc
    85681 tatccatgac gcattaaaag aagtgccaga agtaacgaaa gcagaagtca aactagtctg
    85741 gtatccagca tggacgacag ataagatgag ccgttatgca cggattgcgt tggggatacg
    85801 atagaaatct tggctgacta gctatgagaa gcaaatagga aaaacaaaaa agccttttat
    85861 cctttctaga agaaaagaga taaaaggttt ctttgtttat aaaattttgt ctgctatatt
    85921 ataagttaat taaataaatg tgttctattg ataaaggaca ttcaaatccc tttccttttg
    85981 gaatccttct tttttacatc agtttgattt tcgtatagta ttctttctca tcttcacgat
    86041 aaatcgcagc cccatttttt tgttgtgctt taagactgcc aagatttttt ttatttacag
    86101 acaaaagtgc ctcatcaatg ccgatttttt ttgcctcttg tagtactaat gctaatcctt
    86161 gtgtggcata tcctttttta cgataattag gggagatgcc gtaaccaata tggccagcgc
    86221 cgtttcttaa tgtttcgttc agtttatggc gcaaattaaa aattcctaca tactcatgtt
    86281 catcactttc taaaataaag acggttgcgg gaacgtaact ctcaggaaga tcgattccta
    86341 aagacgtttt ttcgcatttt tttacataat ctaaaaattc agcaaatgta tagccataag
    86401 cattgttgat aaatccactc tcatttggtc caaagctagt ttgtagctga taagctttct
    86461 ttaaatctgc ttcccatagt ttgatcagtt tcatcgtatt cacctcttta agtaacctta
    86521 ttcatttgtt ttctactata gcactattgt tgtaaatagc catgcctatt tttatttatt
    86581 gtgtcatatc gtattaagaa tcatcgaaca atacaaatat gaattacaat aaaaaaatta
    86641 tttgaagttc gttcattcgt tacttatgat ctctagaaga aagaaaacgt gaattacttc
    86701 agcgtctaga attccagaag gatagtaatc aacagaagaa ataatcaata tccaaatgaa
    86761 agaagatagc tgaatcgaga aaaagaggaa aaaagaaatg aagaaaaaaa taaaatagtt
    86821 actaatccca gagtcgttcg tttaacattg aaatcgatca tatggataaa acagattttt
    86881 tcaccaaccc tcaatagatg atggagttgc tcggatgaat tatttgtgta gatggcataa
    86941 gggagggcgt acagaaatga aaaagaacaa aaatatgttc ttgacaaaaa acacatgtaa
    87001 tcctaaacta aaagatgtga tgcggctatg gcggaattgg cagacgcgct agcttcaggc
    87061 gctagtgagg gtgacctcgt ggaagttcga gtcttcttag ccgcataatt tgttttttta
    87121 taaagagaag gtacacctgg agcgatgaga aagaaaataa catattatct aaaagaatat
    87181 aggaaaaatt ttaaacaacg gaaccagatt caaataaatc aaaaaaaatt tgtgtgaagg
    87241 tgggacagtt taaaacctaa tagattgatt caaatagtag atttatagat tctgatacaa
    87301 gaaatactgg caaacgatag ttaaggaata aagactaaac ttaaactcaa aaagaggtca
    87361 tgcgaaagaa taaatcgcat gacctcttac tatattttga atgtgtttaa acgttcaaat
    87421 aatgaatctt agtaccaacc gtttgcttcc cagaatgctt tcgccgcttc ccaagaacca
    87481 taacgtgaag taacatattg ttctgctact ctttcttggt ttgcagcaga gtagtcacca
    87541 tttaagtaag aagaatctaa ttggtaacgg ccgatgtaac gtccgttcgt tgctgtgtaa
    87601 gaaccgcttg attctttttg tgcgatccat tcttttgcag agcttgtact tgctggagta
    87661 gcagcagttt ctgttacagc aggagcaggt tgtgtttcag caacaggcgc tgcttgtgtt
    87721 tccgtttgaa ccgtttcttg tacaacaggt tgttcagctt gaacaggttc agcttggact
    87781 ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc ttctgttggg
    87841 attgttaatt gttgtcctga gtagatcagg ttaatatcag aaattgaatt tgattctgca
    87901 atggcattga tcaaggagtt atcaccaacg tatttttgag aaatcgttga tagagtatca
    87961 cctgattgaa cggtataagc ttcgtccgca tgagcagtcg ttcccatgaa gaatgcagcg
    88021 ccggcagcca aagttgttcc aaaaagtaaa gttttaagtg atgtcatgaa aaatctcctt
    88081 tattcgttaa aaagttttat ttgttgtgtt caacgaggaa tactttaaca cgtgaaaata
    88141 tttcataggt aaaagaagca tgacaattta tgacaaatcc acgttttttg taacagaatg
    88201 atgttcgctc gatgtgaaaa atgatgagga atttcacata atgcagaaat aatgggatta
    88261 gaacaggtgt tctgatgaga aaagaaatgt ataaataact tgaaagaaat tgcttttgtt
    88321 catcatttta tttacatatt gcgtatattt tttgtatttt tgcttgagat gttacaataa
    88381 aagaaaaagg aggcggttcc aatgagtaaa agataccaga acatcatggt agcagttgac
    88441 ggatcaagac aatcgatcaa agcgttcgct gaagcccttg acttagcaaa ggacaatgaa
    88501 gcccacttat ttatcgtgtc aatcatcaac aaagttgagc tgacccatag tgcctatgcc
    88561 ttttcaaaaa tctatgcaga tgaaaaacaa agaacagaag tagccatgct gaaaaaaatc
    88621 aatgatgcca aagagtacgg cattgctgag atccacgcaa tagttgaaac tggagatcca
    88681 agaaatttga ttgccaatgt catccctgga caagaaaata ttgatttgat cgttatggga
    88741 gcgacaggaa aaggtgctat acaacaagca ttagttggat cgaccgcttc ttatgtcgta
    88801 tcacatgccc cttgcagtgt gttggtcgtg aagtaatgag ataagggagg aatagaaaat
    88861 ggttcgtcct caagaagtcc gtgcgccaaa agaaaaaata gaaattttag caattattga
    88921 agacggcaca caaacaaaga agggatactc gatcgcttta gtaaagtgga aaggaaaaaa
    88981 aggtgttgct ctgagatggg atggagataa ccaacaagat aaaggattcc ctgtcacagc
    89041 taacggttat catccagcgt ggtttgttct gcctgataaa tttatggaat tatacagcta
    89101 tgattatgca agagccgcag catcaatcaa agcattagat aaattaacag aagagcagaa
    89161 tgaaatggag gaaaaataaa aaagacgaac caatcagcct ttagaaaact gattagttcg
    89221 aggagtaaaa atgaaaaaag gttatttggg atgaaactag aataatgcgt ataacttaaa
    89281 taaaacttaa agcaataaaa tggcaagagg gtgaggcaaa tggaagcagg aaaagtatta
    89341 caggaagtaa aagcactatg tgcagataaa aaatatgaag aagcgaaaag cctgatcgaa
    89401 aagaacaaag aaaatttgga agaaaagtac ccgatcgcac aagaatttat agaattaaaa
    89461 caaggaacga tccttcaccg attcaaacat ttttttggta taacagaata gaaaaaacga
    89521 ttaaagatcg gcagaaattt gctgcatctt taatcgtttt ctttttaaac atgcgaggga
    89581 ttcaattctg attcttatat aaaaaataga tccaaataat aagaaaaatt actgaaagcg
    89641 tcaattcgat gatgtgtgtt attaatacag acgttgtttc ccaatgccta aaagaaaaaa
    89701 tttgataagt aaaatataca gcaggtaaag aggccagtaa aactgaaaac ttcttttttg
    89761 tccattttat gtaatagaaa agaatgctta tcataataaa aaaaggaata taaaaaacaa
    89821 attgaccgat cgcgatcata ctattgcctc cgataaataa agtccatgat acaaaataac
    89881 actttgtgcg aaatggcgca agcagttttg tggatttatc cggtaaaatc tgtatcgatt
    89941 ttgaaattag aggagaaact tgctataatc aaagtgaaag aaggagtggg attttcatgg
    90001 atcatgtagt gttagaaatc gatttgcatt tgatcaataa tattcgggtg atttactatg
    90061 ttgtaagtaa cagtgtcgaa caacgtgtac tgacgaataa aataaatggt attttagcaa
    90121 agaaagacgt tcaccgattc aataatggtg aaggaagtta ttatagtatc ccagtagaaa
    90181 aaattattta tacgacagtc aaagtcagag aagatctaga taccaaaaaa gcatatgaac
    90241 ctatttttac aacttattga ttgacagcag atggaggaaa aaaatgaatc catatgaaca
    90301 acttattcaa gatttgatcg atggaaaaat cgaaaagatt gatgtaaaaa gagaagaatt
    90361 aatggctttt cgagaagcgt ggctgaagtt ggagaatcgt aaatatgtgg tgggaaaagc
    90421 ctcactgaac ggcaatgtga cttatcatta cgatccgaca cgtttatttt agtatgaagt
    90481 attcagaaaa gaaccaaagg aggacgatgc agcagcattg tcctcctttg attttacaaa
    90541 agtgaaagtt ttacttattt gaatagattt ttaaatttgt ttttgacact gtcgatattt
    90601 acatctttta aaagattttg ggctttctct ttataaggtc ctaattgttc tttatgttct
    90661 tcgataaatc gttttgcgcc atcgaaattg ccgttcttaa tcatttcctt gactttttga
    90721 aataattctt cttgattcat tttgaacaca tcctttgcta tcaacatgat tttttacatg
    90781 gacaaaacga gaaaaactga tttatccaca cgtcaataat atcaaataac gatcattttt
    90841 actaaaaaaa cactcgattc tcgcttctaa agtaggagaa aaatctccaa cttgggacgg
    90901 atggaatttc tttaactgac ccatatactg aatatataac aaggaggtag aaagatgaaa
    90961 gtgattaaat atttgattgg actggcggcg attagtggta ttttgctatt tggactgaaa
    91021 tattatactg aaggaagtta tggagaaatc cctggtatac ttgatcagct aaatccatta
    91081 gttgaaaaag gagaggtcta tgtaaagaca caaaaaccag aggaagtaaa tgaatatggt
    91141 acagcaaggt atgtgcagaa agctgctgat gccaatggga aagaacgaac ggttgaattt
    91201 aatggtcttt ctgtgttaaa agaaaatcat tatttgaaaa ttagcaataa aggagcacac
    91261 atagaaacgt atgaggaagt tcctagagaa aaagttccgg agaaagcatt agcaattatt
    91321 gggtaaatca gtttttgtat taagaatacg cccgatttta ttattcataa tttaattatg
    91381 taaactaaat acaagtagta aaaataatac aataatcccc ctgcaaaaag attgttcttt
    91441 tacagggggt tcattttttc agcaagcaca tcaaattggg atggcagggt tcgaacctac
    91501 aattgcttga ttcagagtca agtgccttac cagtttggct acatcccatt tattcaacaa
    91561 aataatcata gcactttcct aaaaaaacgc aagttatttt tcttgatcgg atagttgttg
    91621 ctttactcgt aaaaggcggc gtactagttt cttttctaat ccagcaggaa gggcaatcag
    91681 ttctgctact gacaagacgg tgtccaataa ctgatattct ttgacaggtt cattcataat
    91741 atgatagatg tttgctaaac gttcaatata ttcaaagcga gtatactctg ttcgagcaat
    91801 tagaaatttc aataaatcgg tggctttctc gtactcttga tctgcgtaat aaacaagaga
    91861 ttgacgatac aatttgattt ccagttcatt gagggtagca ggtctgcgac gctcgttcca
    91921 ctcattaata gagactttac cttgaacatt tcccttgcag tcacggacaa aaataatcgg
    91981 aatttcattt ggatccattt ttttatgtgt gaatttttcc attatctacg ctcccttcaa
    92041 aggtttggta tcttgagtat aagggatttt tgtttgaatt actagctttt ctccaaagtt
    92101 agcatctgtc cacccattgc agtgaaaatc gccgaaaaat cgttgatcgg tactgctcga
    92161 ttttttacac catagggatc gttgacataa atattttctt tgtcatatcc agtgactaca
    92221 caagcatgaa caagaggagt gactcttact tctccattat ttgtggtcca agtcataaaa
    92281 tcagtttcat ctgggacttc aaaattgata gtcacagaag tccagacagg atttccttta
    92341 gatagtgctt ttttgatatc atcgagttga gcatcggttt ttggaaaagt gtgataatcc
    92401 gtttggacga tttgctgagc aagggaggca actgggggaa catagacgac catagcccag
    92461 tctccgccac tgatatcacc aacaaatcct tcattaggat cgccatgagt cccatctgca
    92521 ttgaaaacag ggacgtaatc caattgctca gctaactggt ttttagttgt ttcataacca
    92581 taaaaagata aaagcataga aagcgaagtg acttcacagc cattttctaa agaaggagaa
    92641 tcaaactggc tctctaatgg aacatctagt aaaacttctg ctttctcttt tgtctttttc
    92701 gttttttgtg ttgaactttt ttgtgcagtg ttcattgtat gttctggaga atggatatgt
    92761 aggtaataac ttgtaccgct taataaaaga gcagctgtgc cccctagaaa caatagtttt
    92821 ttgaacgttt ttttcttcat ttgaaatctc ccttttcagc tgtttatctt tcctattata
    92881 aatgtatttt tattggaaat ggaagtatta tattttaata tgtaggaaat aattagtttt
    92941 taaggaatat gttttaattt tttgaaaaaa attcttctaa atacaacagc tgttttaatt
    93001 gtgaaaacga aaacagtacg ctataatgcc tgtaaggttg atttttaact gtaaataaaa
    93061 atcaatgagt taaatctcca aagaaatagc agtggtaatg tggagaaaag ttccagcagt
    93121 ttaggaggat tttgatgacg gaagaaatga ttaatttagg agaacaatat gcatgcaaac
    93181 caattggttt tacaaaaacg gtcattggtg aagttgtatc aaaaatgaca aactgcgccg
    93241 tggtcaaagt tgcccaatgt gctgcagagg accaagaatt attagatgaa aaagcaagca
    93301 tggttgttgc aaaatatgat acatttgaat aaagaaaaca attggacgtg cggcgtgctg
    93361 caagtccaat ttctttgaga aggtaaatca gtattctttt cctattttct ggagaagaaa
    93421 aagcgaggcg agttgtcatg gtaacattct ttacggttct attgttttta ttcgttgttg
    93481 tgatgttttt atttctttgg ctggttcgaa aagaaattat ttatcggact gtgcgaaatc
    93541 gatgggtcta cttggtgata cccttcttag tgtcactagt catttggtat acactcatca
    93601 gtcatcctac agcagatgaa ttggcaaaag ggatcttgtc agcaatgatt tttattagtt
    93661 ttttgttgga cagtagagga atcacagaag aaggacttgt tttgaatagc tttgacaaaa
    93721 aaggtgttcc tttttctgag atcaataaaa ttgtattata tcagccaaaa ggttctaaaa
    93781 tcgtaaaaat gaattttttc cgaaacggat ggcgaggacc gatgctgaaa ttttctgcat
    93841 cattagaaga attagttcca tttttgtccc aacgcttaaa cgatgaggca gagattgata
    93901 ttatgattga tccagaataa aaacagaggt tgcgacaaga tgtttgtcac aacctctgtt
    93961 ttttatttca aataaaatta tccaaatacg cggaagcgat cggcatcgtt catgtattct
    94021 ttttcctcag ccggtgtttc tggtgaaccg aatactaatt gcgaacgtag tttccagttt
    94081 gaagggatat tccattcttt tgcaactgct tcgtcaataa ctgggttgta atgttgcaag
    94141 tttgcgccca atcctttatc aactaatgct acccaagtat tagctgttgc gattccgttt
    94201 gattgttctg accaatctgg gaagttgtct gcatataatt caaattgttc ttgtagacct
    94261 tttaccacgt cagtatcttt gaagaataat actgtaccat aaccattttt gaaaccagct
    94321 aatttgtttt gtgtatttgg gaaagcttct gctggagtca atgggcgaag tgcttcttca
    94381 gtgatttccc aaagtttttc atgtgcatct ccgaaaagga taactgcgcg tgttgattga
    94441 gcattgaaag cagttgggct ttctttaatt gcttctttga tcaaagttgt taattcttca
    94501 ttagaaagtg aaacattacg tcctagatga tagattgaac ggcggttttt caaagtgtca
    94561 gtaaaagttg tcataataaa atttctcctt tgatttgcgt tttgcatttt tttaattgat
    94621 tacgaaatga agtatagcac cttacaaaaa gtaagtaaac agatatgctc acaaaaaata
    94681 agcgaagttc aaaaaacaat tttaataaga aaaatcgtta tgtaaggatt ttcttagtta
    94741 tcgctttcat tgttgtgttt taggaatttt tgcttatttg aaaaaaacag ctgatatata
    94801 att
//
LOCUS       DASNNU010000002        79662 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.002, whole genome shotgun sequence.
ACCESSION   DASNNU010000002 DASNNU010000000
VERSION     DASNNU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.002"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            14..646
                     /locus_tag="U8116_000096"
     CDS             14..646
                     /locus_tag="U8116_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433120.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(780..905)
                     /locus_tag="U8116_000097"
     CDS             complement(780..905)
                     /locus_tag="U8116_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8433121.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1039..1932)
                     /locus_tag="U8116_000098"
     CDS             complement(1039..1932)
                     /locus_tag="U8116_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEN8433122.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2114..3040)
                     /locus_tag="U8116_000099"
     CDS             complement(2114..3040)
                     /locus_tag="U8116_000099"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEN8433123.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3125..3886)
                     /gene="pflA"
                     /locus_tag="U8116_000100"
     CDS             complement(3125..3886)
                     /gene="pflA"
                     /locus_tag="U8116_000100"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEN8433124.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4130..6361)
                     /gene="pflB"
                     /locus_tag="U8116_000101"
     CDS             complement(4130..6361)
                     /gene="pflB"
                     /locus_tag="U8116_000101"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEN8433125.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6638..9088)
                     /gene="parC"
                     /locus_tag="U8116_000102"
     CDS             complement(6638..9088)
                     /gene="parC"
                     /locus_tag="U8116_000102"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEN8433126.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9100..11145)
                     /gene="parE"
                     /locus_tag="U8116_000103"
     CDS             complement(9100..11145)
                     /gene="parE"
                     /locus_tag="U8116_000103"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEN8433127.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11311..11745)
                     /locus_tag="U8116_000104"
     CDS             complement(11311..11745)
                     /locus_tag="U8116_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEN8433128.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11872..12510
                     /gene="plsY"
                     /locus_tag="U8116_000105"
     CDS             11872..12510
                     /gene="plsY"
                     /locus_tag="U8116_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEN8433129.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12642..13517)
                     /locus_tag="U8116_000106"
     CDS             complement(12642..13517)
                     /locus_tag="U8116_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEN8433130.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13599..14390)
                     /gene="codY"
                     /locus_tag="U8116_000107"
     CDS             complement(13599..14390)
                     /gene="codY"
                     /locus_tag="U8116_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEN8433131.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14408..15808)
                     /gene="hslU"
                     /locus_tag="U8116_000108"
     CDS             complement(14408..15808)
                     /gene="hslU"
                     /locus_tag="U8116_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEN8433132.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15822..16370)
                     /gene="hslV"
                     /locus_tag="U8116_000109"
     CDS             complement(15822..16370)
                     /gene="hslV"
                     /locus_tag="U8116_000109"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEN8433133.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16407..17312)
                     /gene="xerC"
                     /locus_tag="U8116_000110"
     CDS             complement(16407..17312)
                     /gene="xerC"
                     /locus_tag="U8116_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEN8433134.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17510..18886)
                     /gene="trmFO"
                     /locus_tag="U8116_000111"
     CDS             complement(17510..18886)
                     /gene="trmFO"
                     /locus_tag="U8116_000111"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEN8433135.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18855..20933)
                     /gene="topA"
                     /locus_tag="U8116_000112"
     CDS             complement(18855..20933)
                     /gene="topA"
                     /locus_tag="U8116_000112"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEN8433136.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21055..21912)
                     /gene="dprA"
                     /locus_tag="U8116_000113"
     CDS             complement(21055..21912)
                     /gene="dprA"
                     /locus_tag="U8116_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEN8433137.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21968..22735)
                     /locus_tag="U8116_000114"
     CDS             complement(21968..22735)
                     /locus_tag="U8116_000114"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEN8433138.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22728..23588)
                     /gene="ylqF"
                     /locus_tag="U8116_000115"
     CDS             complement(22728..23588)
                     /gene="ylqF"
                     /locus_tag="U8116_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEN8433139.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23848..24246
                     /locus_tag="U8116_000116"
     CDS             23848..24246
                     /locus_tag="U8116_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN8433140.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24525..25025
                     /locus_tag="U8116_000117"
     CDS             24525..25025
                     /locus_tag="U8116_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN8433141.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(25108..25659)
                     /gene="lepB"
                     /locus_tag="U8116_000118"
     CDS             complement(25108..25659)
                     /gene="lepB"
                     /locus_tag="U8116_000118"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN8433142.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25811..26038)
                     /locus_tag="U8116_000119"
     CDS             complement(25811..26038)
                     /locus_tag="U8116_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEN8433143.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26035..26553)
                     /gene="msrA"
                     /locus_tag="U8116_000120"
     CDS             complement(26035..26553)
                     /gene="msrA"
                     /locus_tag="U8116_000120"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN8433144.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26573..27286)
                     /locus_tag="U8116_000121"
     CDS             complement(26573..27286)
                     /locus_tag="U8116_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEN8433145.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27289..28185)
                     /locus_tag="U8116_000122"
     CDS             complement(27289..28185)
                     /locus_tag="U8116_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN8433146.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28343..29185)
                     /locus_tag="U8116_000123"
     CDS             complement(28343..29185)
                     /locus_tag="U8116_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN8433147.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29317..29970
                     /locus_tag="U8116_000124"
     CDS             29317..29970
                     /locus_tag="U8116_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEN8433148.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30033..30560)
                     /locus_tag="U8116_000125"
     CDS             complement(30033..30560)
                     /locus_tag="U8116_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN8433149.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30580..31527)
                     /locus_tag="U8116_000126"
     CDS             complement(30580..31527)
                     /locus_tag="U8116_000126"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEN8433150.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31539..33425)
                     /locus_tag="U8116_000127"
     CDS             complement(31539..33425)
                     /locus_tag="U8116_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN8433151.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(33585..35663)
                     /locus_tag="U8116_000128"
     CDS             complement(33585..35663)
                     /locus_tag="U8116_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEN8433152.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35676..35951)
                     /locus_tag="U8116_000129"
     CDS             complement(35676..35951)
                     /locus_tag="U8116_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433153.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36313..36768)
                     /locus_tag="U8116_000130"
     CDS             complement(36313..36768)
                     /locus_tag="U8116_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433154.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36922..38130)
                     /locus_tag="U8116_000131"
     CDS             complement(36922..38130)
                     /locus_tag="U8116_000131"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEN8433155.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38242..38574)
                     /locus_tag="U8116_000132"
     CDS             complement(38242..38574)
                     /locus_tag="U8116_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN8433156.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38722..39597
                     /locus_tag="U8116_000133"
     CDS             38722..39597
                     /locus_tag="U8116_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN8433157.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39679..40260)
                     /locus_tag="U8116_000134"
     CDS             complement(39679..40260)
                     /locus_tag="U8116_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEN8433158.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40266..41525)
                     /locus_tag="U8116_000135"
     CDS             complement(40266..41525)
                     /locus_tag="U8116_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN8433159.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41527..42573)
                     /locus_tag="U8116_000136"
     CDS             complement(41527..42573)
                     /locus_tag="U8116_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433160.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42808..43083)
                     /locus_tag="U8116_000137"
     CDS             complement(42808..43083)
                     /locus_tag="U8116_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEN8433161.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43326..44636)
                     /gene="der"
                     /locus_tag="U8116_000138"
     CDS             complement(43326..44636)
                     /gene="der"
                     /locus_tag="U8116_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEN8433162.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44818..46047)
                     /gene="rpsA"
                     /locus_tag="U8116_000139"
     CDS             complement(44818..46047)
                     /gene="rpsA"
                     /locus_tag="U8116_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEN8433163.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46182..46862)
                     /locus_tag="U8116_000140"
     CDS             complement(46182..46862)
                     /locus_tag="U8116_000140"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEN8433164.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46911..47546)
                     /locus_tag="U8116_000141"
     CDS             complement(46911..47546)
                     /locus_tag="U8116_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN8433165.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47612..49015)
                     /locus_tag="U8116_000142"
     CDS             complement(47612..49015)
                     /locus_tag="U8116_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEN8433166.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(49002..50033)
                     /locus_tag="U8116_000143"
     CDS             complement(49002..50033)
                     /locus_tag="U8116_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8433167.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50064..50285
                     /locus_tag="U8116_000144"
     CDS             50064..50285
                     /locus_tag="U8116_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEN8433168.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50450..51187)
                     /locus_tag="U8116_000145"
     CDS             complement(50450..51187)
                     /locus_tag="U8116_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEN8433169.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51340..52680)
                     /locus_tag="U8116_000146"
     CDS             complement(51340..52680)
                     /locus_tag="U8116_000146"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEN8433170.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(52917..54440)
                     /locus_tag="U8116_000147"
     CDS             complement(52917..54440)
                     /locus_tag="U8116_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEN8433171.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(54818..55429)
                     /locus_tag="U8116_000148"
     CDS             complement(54818..55429)
                     /locus_tag="U8116_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN8433172.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(55552..55677)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55759..56475)
                     /locus_tag="U8116_000149"
     CDS             complement(55759..56475)
                     /locus_tag="U8116_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN8433173.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(56481..57050)
                     /gene="scpB"
                     /locus_tag="U8116_000150"
     CDS             complement(56481..57050)
                     /gene="scpB"
                     /locus_tag="U8116_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEN8433174.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(57034..57831)
                     /locus_tag="U8116_000151"
     CDS             complement(57034..57831)
                     /locus_tag="U8116_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEN8433175.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(57801..58172)
                     /locus_tag="U8116_000152"
     CDS             complement(57801..58172)
                     /locus_tag="U8116_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN8433176.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(58191..59078)
                     /gene="xerD"
                     /locus_tag="U8116_000153"
     CDS             complement(58191..59078)
                     /gene="xerD"
                     /locus_tag="U8116_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEN8433177.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(59174..59647)
                     /locus_tag="U8116_000154"
     CDS             complement(59174..59647)
                     /locus_tag="U8116_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN8433178.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(59752..60615)
                     /locus_tag="U8116_000155"
     CDS             complement(59752..60615)
                     /locus_tag="U8116_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN8433179.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(60811..62910)
                     /locus_tag="U8116_000156"
     CDS             complement(60811..62910)
                     /locus_tag="U8116_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN8433180.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(63130..64239)
                     /gene="rpoD"
                     /locus_tag="U8116_000157"
     CDS             complement(63130..64239)
                     /gene="rpoD"
                     /locus_tag="U8116_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEN8433181.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(64263..66104)
                     /locus_tag="U8116_000158"
     CDS             complement(64263..66104)
                     /locus_tag="U8116_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN8433182.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(66286..68601)
                     /locus_tag="U8116_000159"
     CDS             complement(66286..68601)
                     /locus_tag="U8116_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN8433183.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(68786..69646)
                     /locus_tag="U8116_000160"
     CDS             complement(68786..69646)
                     /locus_tag="U8116_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEN8433184.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(69705..70226)
                     /locus_tag="U8116_000161"
     CDS             complement(69705..70226)
                     /locus_tag="U8116_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEN8433185.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            70504..71265
                     /locus_tag="U8116_000162"
     CDS             70504..71265
                     /locus_tag="U8116_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN8433186.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            71291..73882
                     /gene="mprF"
                     /locus_tag="U8116_000163"
     CDS             71291..73882
                     /gene="mprF"
                     /locus_tag="U8116_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN8433187.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(74070..75257)
                     /gene="tuf"
                     /locus_tag="U8116_000164"
     CDS             complement(74070..75257)
                     /gene="tuf"
                     /locus_tag="U8116_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN8433188.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            75625..75981
                     /locus_tag="U8116_000165"
     CDS             75625..75981
                     /locus_tag="U8116_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN8433189.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(76222..77226)
                     /locus_tag="U8116_000166"
     CDS             complement(76222..77226)
                     /locus_tag="U8116_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEN8433190.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            77392..78243
                     /locus_tag="U8116_000167"
     CDS             77392..78243
                     /locus_tag="U8116_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN8433191.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            78260..78505
                     /locus_tag="U8116_000168"
     CDS             78260..78505
                     /locus_tag="U8116_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433192.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            78692..79396
                     /locus_tag="U8116_000169"
     CDS             78692..79396
                     /locus_tag="U8116_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433193.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT        21930 a        16726 c        13683 g        27323 t
ORIGIN      
        1 atttggagat attatggaaa ttcaacgtag aaccactaat tttttacatt taggctcttt
       61 ttttgcaact ttgacgatct tattttttct atgttattca taccttaaga cctcacatgc
      121 gccttctagt gcattaggct ggttgctttt ggatcattct gatatcacag aagccgaaga
      181 aaatccgttt tttttgattt tgtcttctct ttgcggctta ctgttttctg tttatttcgg
      241 tgcctatttt tttcatcgac atcctggtac gttaaacgaa gcttcaagcg tattgattgc
      301 atttaaaacg ttttcttttt tagcaattct tttttattcg tttaatcaca atacgctttt
      361 cttctttatc atgaatggtt tagtcgtgat tttatccatt tttacttatt ttattttttc
      421 actcattttt agccaattga aatgtccgct cttctttcga ttagttcctg tcacctgttt
      481 tatttacagc ttgttttcct tttttgtcct tgctttaatg ccactgacca acccttcgac
      541 gatccaagtc gcaggaaatc tgttggatat gctcttcatc gtcagtctag gtgtatttat
      601 gtggcaaaga aaaaacaaac aaaataaaaa gcattttgaa gcctgactat tacgacttca
      661 taaatgcttt tttagtttta aacacttcaa tctaaaagaa taacccaaga ttggataaat
      721 tattaatgac tcgccattcg tttcttagtg attctggatt atcaaatgga cttcctaatc
      781 taatcagcta gttcttccca taaaatgcca tacttgactg aatcataata ttgattattt
      841 aaaaatctaa cttttcttat cactacttcc tttgtcattc cacatttctc accatttttt
      901 tgcattcctt tgtttccaga ccatgtagtg aatctaatag gtggtagata ggcaaaatac
      961 tcaaataatt cttttaacca taaaggaaga gaaaggctcc tcctgcaaga gtcagtataa
     1021 tttttgctta ctgctctctt aggttttact cactaaaact gttccatatt ctttgatata
     1081 agaaaatgca tgcggtataa atttcttgct cagctcagaa aagatttgtc gtttaagaaa
     1141 ggctatttcc tccattgtca atgaaggatc caatgttggt aattcaatct caaaattgtt
     1201 tttttcgaca ataggtaatt ctaatgtgaa tgattcttct tcctttatag tattcgtcac
     1261 aagtagagac tgatggaaaa tactaggccg gcgattttta tatttattct tacgtatcct
     1321 gtcaacgata acttctattt gatcaaaaaa gtacgattca tttacgtctc caataagttc
     1381 ggctaattct ttgaatgcct tcgttacagg aagctgattc ttctgtacaa aaaagtaatt
     1441 agccaaagta agcgggaagt gttctataga ggaaagtgtg ataaaacggt acttctcatt
     1501 tcctgcagtt gtcctcagca attctgtctc tcggctgttt cttactccat aaaaatacgc
     1561 ttctttccgt tcatcttcga aacaatacgc ccaaataact tctcccatcg ctaatttttg
     1621 ttcatcaaca gagagtctct tcaattgttc agctatggtt tcagcagccg aagtggcttg
     1681 ttgcaagtaa tctttaggat caaatattgg aaagttcaaa tgatatcttc gttcttccct
     1741 cttgattaat cctgcatcga tcaataaatc aagaaaatgg gaaaatttat tttgcgaaaa
     1801 ttcttttttc aattctctta gaatcgtttc ttgttctcga tgttcatata gataatcggt
     1861 caatgaagaa aaaagcggat catcggtcag tttttgataa aagttttcct tattttttga
     1921 ataatagttc atatatgttc tcttttctat ccgttttatt ttacttaaaa ggcttttacg
     1981 taaaaaggag gaagaacaag agtcataatt gactgttatt ctcctccatt tcttcggcct
     2041 ttagtatgtt ctatgtttct ttttatatca atcgcttatc cattcatttt gaatgcttca
     2101 tactaccatt tgattagcta aatacagccg tcaattgagg tacgacttgt tttttacgtg
     2161 aaacgacacc tttaagcaaa gcacggttgt tggtcaaagt tgtttggaaa gcttcttcta
     2221 ctttttcttt tcctttacca gcaacaagta attctgaatc actgtctaag atgtttgtga
     2281 caactaatac gaacagatcg tagccatttg ctttcatttc atcttccatt gctgcttcta
     2341 gctctgcttg tcgatccatc acttcattaa ggtccactgt attgacttga gcgatacgaa
     2401 cgttggaact gcccattggg aaactttttg catccaaatc aagaagaacc gcagctgatt
     2461 tatcacttag atttgttcct gctttcaaca tttcaagtcc ataagtgttt agatcgactt
     2521 cagcgattgc tgcaagttct tttgcagctg cttcatcttc aggcgtacat gttggtgatt
     2581 tgaacaacaa tgtatctgaa atgatagctg agagcatgat tcctgcgatt tctttaggaa
     2641 ttgttacttg gttttcttta tatagtttta aaataatcgt acttgtacat ccaactggtt
     2701 ctgcacgata gtacaatgga ttagctgttt caaagtttgc aatacgatgg tggtctacta
     2761 cagctaaaat atttacttcg ctgatatctg aaatactttg ttggaattca ttatgatcca
     2821 caagcatgac atctgcattt tctgctttag ctgactctac tactcgtggt gcagatagct
     2881 gaaagtggtc taatgcgtat tgtgtttctt cattaggtgt acctagagca actgcttctg
     2941 tttctttacc taattcattt tgcaagtgtg aaaacgcaat ggctgccccg attgcatctg
     3001 tatctggatt ttggtgacca aaaactaaaa tttttgacat gtcattcgct cctttgtgat
     3061 ttctcctatt taataatagc aaaaaaacgg aaagaagcaa tacttatctt ctttccgatg
     3121 cgacctatcg cgttaaatat tttgtatact cttctgtatg aagcaatcgt ttagcatttt
     3181 cgacacgttc ttttgtaggt ggttcgatcc cttctaatgg atatttcaaa ccaagtgttt
     3241 cccacttgta ttttcccatc gtatgataag gaagaatctc tactctgtct acatttgaca
     3301 aggtttgaat aaagttgtct agtcggatca aatattcatc ataatcactt cgctcaggaa
     3361 ctagtacatg gcgaatccaa accggtttgt tgatttttga caaatattga gccatttcta
     3421 aaatattttc attgtcccaa tgcgttagtt ttttatgctc ttcattatcg atgtgtttga
     3481 tgtcaaataa cagtaaatct gtatacttca tcaattcttg aaaacgtgaa aagaaaggtt
     3541 cttcatacgt aaaaggtttt ccgcatgtat ccaatgtagt atgcatcttc cgctcttttg
     3601 cttttttaaa tagttcgatc aaaaaatcga tctgcaatag aggttctcca ccactaacag
     3661 taataccacc tttatctccc cagtaaggtc tgaagcgttc tgctttatct aaaagttcat
     3721 ctgctgtgta ttctttccct ccgccaatat tccaagtatc tggattgtga caaaattggc
     3781 aacgcatgcg gcatccttgc ataaacacga caaagcgcag tccaggtccg tctactgaac
     3841 cgaaagtttc gatagaatga acatagccga ttgttttttc ttccatttaa gttcacatct
     3901 tttctttgat ttcattttaa gtacaagtta gctgtcgaag cgaagctttc tgaacaaaat
     3961 ttttcgcaat tgccgcttcg ttatgtgtaa aatttacagg gacggaacat acaagtcctg
     4021 cccctgtaaa tcgtttcttt caaataagca tactgattat tcgattttga tgcgatggct
     4081 attcaacgtc ctttcgacgc taagtcgaaa aacttttttc acgaactttt tacattctgt
     4141 cgtgtgaagt tctcatcaag acatctaatt gttgttctct tgtcaagtca cggaatttaa
     4201 ctgcataacc agatacccgg atcgtcaagt tcggatattt cattggattt tccattgcat
     4261 cacgcaataa atcattactg aatacattga cattcaaatg ataagctcct tttgagaagt
     4321 atccatctaa gacattacgc aagttttcga ttcttgtatc ttgttctttt ccaagacctg
     4381 aaggattgat tgtttgggta tttgaaattc catccaatgc acagccgtaa ttcattttag
     4441 ctgttgagtt caggctagct aataacccac tttgttccgc attgtaactt ggatttgctc
     4501 ctggcgcaag tggttttcca gcttcacggc catcaggagt atttcctgta tttttaccgt
     4561 aaacaacatt tgaagtaatc gtcaataatg aagttgttgg ttttgagttg cggtaagttt
     4621 tatgacgttt gatttgtccc atgaagtatt ccaagatcca tgaaactaat tcatctgcac
     4681 ggtcatcatc atttccgtat ttagggaagt cgccatccac ttcaaaatca actgctattc
     4741 ctttttcatt acggattgct tttacttttg catatttgat cgctgacaag ctgtctgccg
     4801 catgagagat cccagcaata cctgtagcaa atgtgcgttg caagtcagaa tccatcaaag
     4861 ccagctgagc tgcttcataa gaatatttat catgcatata atgaatgatg ttcaacgtat
     4921 tgacatatag ttctgctaac caatccatca tattcatgaa gttatgtttc acttcttcaa
     4981 agtctagtat ttctgatgtg attggacgat attctggtcc aacttgggcg ccagtcactt
     5041 catcgactcc gccattgatg gcatataata gtgttttagc caagttagca cgagcgccaa
     5101 agaattgcat gtcttttccg acaactgttc ctgatacaca gcaagcaatc gcacaatcat
     5161 cagaacccca ttgttcacga agctgattat cattttcgaa ttgaatcgaa gaactttcga
     5221 tagcgattct gctagcatat gttctgaatc cttctggaag gtgttcagaa tacaaaacag
     5281 tcaagtttgg ttctggtgaa ggtcccatat ttgttaatgt atgaagcaga cggaaatcat
     5341 tctttgtaac taatgaacga ccatctaatc ccatacctgc aatggataaa gtagcccaga
     5401 tagggaatcc tgagaataat tggttatatt caggtgtacg cgcgaatttg accatacgta
     5461 atttcattac taagtggtcg atcaattctt gtgcttcttt ttctgtgatt actccattat
     5521 ccaaatcacg ttggatatag atatcaagga atgctgagat acgtccgatt gacatcgcag
     5581 caccattttg tgatttgatt gctgctaaat atccgaagta tagccattgg acagcttctt
     5641 ttgcatttgc agctggttta gaaatatcat agccatattt cgcagccatt tctttcattt
     5701 gcgctaatgc acgatattgt tcagtgattt cttcacgtaa acgaatcact tcttcactca
     5761 ttgtatgata accagtcata tcatgatctt ttttcttttg ttccatcaaa tagtcgattc
     5821 catacaatgc cacacgacga tagtcaccga tgatacgtcc gcgaccgtaa gcgtctggaa
     5881 gccctgtaat gattttgttt ttacgagcag cacgcatttc agcagtataa gcgtcaaata
     5941 caccttgatt atgtgttttg cgccagtcag tgaaaatatg tgtcaattca tcgtttggtg
     6001 tatagccatt gctttttagc gcattgttag ccatattgat tccgccgaac ggcatgaatg
     6061 cttgtttcaa aggtttgtct gtctgtagac cgacgatttt ttccaaatct ttatccaaat
     6121 agcctgcatc gtgtgaagta atagttgata cgatttctgt gtccatatca tagacaccgt
     6181 tacgctcgaa ttggatatca tttaactttt gcaattcatc ccataatttt tctgttgctt
     6241 ctgttgggcc ttccaaaaag ctgtcatcac cgcgatattc agtgtagtta ttttgaataa
     6301 agtctctagt gtctacgcct tctttccatt tggtaccttt aaatccattc cattgctcca
     6361 ttgtatggac ctcctaaatt tctgttttat gtaacagttt ttacgtgatt agtataacac
     6421 agcaccctat ctttgcaagg gttttcttgt tttttattta acaattttta tgttttattt
     6481 acatatattt aaatcaacgt tgacatacca acatttaagc attgtgaaca ctttcatctt
     6541 tttttgcaaa cgtataagga tttattaaaa atgaatgaaa aagagacgcc acctgtatta
     6601 atacagatgg cgtctcttca ttaaaatact acaacagtta cgactcgatg aaaatctcag
     6661 gagcatcgat aacttcgatg acttctccac cttttttctc atccatcgca aatgatccat
     6721 tcgatgttcg atcacctata gggaaatcag ttgggtcaat cgttaactgc tgcccttttt
     6781 gattcatcaa ggttatttct tttgatcttc cttccgacat atagataata cggtgcggat
     6841 tttttttcag ctcacgcatg accgtcactc cccgcttcgc acgtgaagtt tggctgattt
     6901 cttgagcaag catgcgttta acagctcctc gctgagtaac gattacgatc ggcgtatcac
     6961 cttctgctaa cacaagcagc ccgttgataa tcctatctcc atctttcaaa ttcatagact
     7021 tcactccagc tgcctttgct ccaacaacgg gaacatctgt taaaggatag cgcaaaccca
     7081 ttccttgatg cgttactaag aaaacatcta agcctggctg atcttctttc agatacacag
     7141 caatcagttc atcttcttcc gcctttagct tcatacagct gagcggacgg ctcttatagg
     7201 tgcgccatgg ttcgaactct gtcattcgtg tttgcttgat caatccattc ttgctgatga
     7261 atacaaatgt tttctctgct tgaatttttt gatatgggaa aacagctaaa atcgattcgt
     7321 ctacagacag attggagatt gtttgagaaa tgtgctcacc agcatctttc catttcaaat
     7381 cgggtagttc atggacaggg cggtagatca catttgcttt atttgtgatc agaagaacat
     7441 ggtccaatgt actcaattca cctgagtata gcaagtagtc gccttctcgc atcccgattt
     7501 cttctggttt ggaagcacta taagaacgaa ggcttgttcg cttcacatag ccttctcgcg
     7561 tcacactgac aattacgtct tcttgtgcca ctaagacttg cgtatcaatt ttgatttctt
     7621 cgatttcgtc ttcgattgtc gttagacgag cggaagaata attttttttc acttctcgca
     7681 attctttttt cataacagag aaaagctctt tgtcatttga aaggatttta ttcaactctg
     7741 tgatttgcgc aatcaagctt tcggattcct tttgaagctc tgtgatatcc gtgttcgtca
     7801 aacggtataa ttgcaatgtg acaattgctt ctgcttgttc ttctgtaaat tgaaagacgt
     7861 cgactaaatt ctttttggcg tcttttttgt ctttactttc acgaatcgtt gcgattactt
     7921 catccagaat cgataaagct ttcatcaatc cttctacgat atgctgtctt ttttgagctt
     7981 tggctaaatc gaattgacta cgttttgtta tgacttgctt tcgatgctcg atataacttc
     8041 ttaaaatgtc tttcagtcca acttgatgcg gagtcatatg gtcgatagcg accatattga
     8101 aattgtaatt gatctgtagc tcagtgtttt tgaacaaata attcaaaatt ccttgagcat
     8161 tggcgtcttt tttcaattcg acgacaattt gcagacctgt tctatcactt tcatctcgta
     8221 cttcagctat tccatcgatt ttcttattca aacggatctc atccattttt ttcaccaagg
     8281 tcgctttatt tacttcatat gggatttctg tgatcacgat ttgctgttta ttacccttca
     8341 ttgcctctat ctttgttttc gaacgaagaa taacttttcc tcgtcctgtt tcataagctt
     8401 ttttgatttc atcttttcct tgcaagatcc ctcctgttgg aaagtcaggt ccaggaatat
     8461 attccatcaa tttttcaagg ctcgcttgtg gatgatcgat catatagatt gtgccatcga
     8521 tcacttcagc taagttatga gtcgggattt ctgttgcata tccagcagat atacctgtcg
     8581 atccattgac gagaaggttt gggtatcgag ctggaaggac tgttggttct ttttctgtat
     8641 catcaaaatt ccacacgaga tcgactgttt ctttctcgat atctgctagc atctctccac
     8701 ttaacttgga taatctggct tcagtataac gcatcgcagc aggtggatca ccgtccatac
     8761 tcccgttatt cccgtgcatt tcgatcagta cttcccgcaa cttccagtct tggctcatgc
     8821 ggaccatcgc ttcataaata ctgatgtcac catgaggatg ataattcccc atgatatttc
     8881 cgacagattt tgctgattta cggaatcctt tgtcatacgt attgccatct ttgttcatgg
     8941 caaataatat ccggcgttgc actggcttca atccatcacg gatatcaggc agtgcacgtt
     9001 cttggataat atatttggaa taacgtccga agcggtcacc catcacttct tctaaagtta
     9061 attcttgtac ttcttgtcga ttttccatca ggtgacctcc tattctactt caaataactg
     9121 attgttttca tttttatctg ctcgttcttc atctaataaa tcattagaaa ctgaaggaga
     9181 gatttcagta tcctcttttt tatctaagat actgccatct tcttcgagcg taaattgcac
     9241 gtgattttcg atccattttc ttcgaggttc tactttgtca cccatcagtg tggttactcg
     9301 acgttcagct tgcgcagcat catcgatacg gacgcggatc aacgtacggg aagtcggatc
     9361 catcgttgtt tcccacaatt gttccgcatt catctctcca agtcctttat atcgctgcag
     9421 catatatcct ttgccgactt ttttaatcat tgctgccaat tcgtcatctg tccacgcata
     9481 ttcaatgact tgcttctttc cttgtccttt ggatacttta tataaaggcg gtaacgcgat
     9541 atatactttc ccagcttcaa tcagcggttt catataacga taaaagaaag taagcagtaa
     9601 tacttgaata tgggcgccgt ctgtatccgc atcggtcatg atgattactt tgtcgtaatt
     9661 acagtcttcg atagaaaact caggaccgac tcctgcacca atcgtataga tcatcgtatt
     9721 gatttcttca tttttcaaga tatcttgcat tttagctttt tcggtattga tgacttttcc
     9781 tcgtaaaggt agaattgctt ggaactttct gtctcttcct tgtttagcag atccgccagc
     9841 agaatcccct tcgactaaat acaattcatt tttcttaggg tttcgggatt gtgcgggtgt
     9901 caattttcct gaaagaagag attcgccttt tttacgcttc ttcccatttc ggctttcttc
     9961 tctggcttta cgtgctgctt ctctcgcttc tctggctttg attgctttac ggaccaacat
    10021 ctggctcatt tcgctatttt cctgcaaata aaagcccatt tgttcactga tgacgttatc
    10081 tactacggta cgagcaactg gcgtaccaag tttttctttt gtttgccctt caaattgcag
    10141 aagattttca ggtacacgga tagaaagtac agcagctaac ccttctctga aatcactacc
    10201 ttccagattc ttgtcccgtt ctttcagcaa accaactttt ctcgcatatt cattataaga
    10261 tttcgtcatc gccgctttca taccagcttc atgggttccc ccatcttttg tccgaacgtt
    10321 attgacaaaa gataaaacat tttctgaata accgtcattg tattgataag cgacctctac
    10381 ttcgatacct tctttttctc ctgaaaaata aacaactggt gtcaatgtat ctttttcttc
    10441 attcagataa tcaacaaatt ctttgattcc ttcttcgtaa tgaaaaattt ccttgactgg
    10501 ttcttctcca cgaagatcag aaagagagat tttaacgcct tttagaagaa aggcagattc
    10561 tcttaaccgt tcggctaaaa tttcatatga aaatttcgtt gtactgaata ttgtatcatc
    10621 tggtaaaaaa tggacagtcg tcccgtttgc tttttttgtt tttcctatct ttttcaaagt
    10681 accgtcaggt ttaccgccgt ttttaaattt ctgctgatac tctacgccat ctctaacgat
    10741 cgttacaatt agccatttcg ataaggcatt taccacactg gctcctacac cgtgcagtcc
    10801 accagaagtt ttgtaccctc cttgaccaaa ctttccgcca gcatgcagca cagtaaaaat
    10861 cacttcaact gttggtattc cggaagcatg cattcctaca ggcatcccac gtccgctatc
    10921 tgcaacagtt atactattgt cttcatggat ggtcacatca atctcactgc cataacctga
    10981 taatgcttcg tcaactgcat tatcgacgat ttcatagact aaatgatgca agcctcggct
    11041 gtcagtggat ccaatgtaca tccctggtct ttttctaact gcctctaatc cttccaatac
    11101 ttggatggac gagtcattgt attcgttgtt tacttttttt gccaaggcaa acgctccttc
    11161 aataatactt tgcggcaaaa ccgcaccacg attatcatac tagaaaaggt cgtgaaaaaa
    11221 aagagggtag acgagaaatc aattttaatt atctcctaac cgctttttcg acattcccct
    11281 ctatcgatca tctcttcttt gtggtcattt ttatttttgg ttttctttca acttaccaag
    11341 ttcaatctta atacaacgat ccattacgat atctgtacgt cctgcttttc ttagcatctc
    11401 agctgcttct ttgttttcaa tccctaattg tgcccagaac actttagcat cagttttcaa
    11461 aaaatcacct gctatttcag gtaaaaattc actccgtcta aatacatcta cgatatcgat
    11521 tggatcatcg atttctgtca aattcgcata gactttctcg cctagaattt cttcaccagc
    11581 caataatgga tttactggta tgattttata tccattgtct tgcaatacct gtgcaaccat
    11641 gtaacttgtt cgttctggtt tattgctcaa gcctacgacg gcaatatttt ttgcttcttc
    11701 caaatagttg aaaatcgttt cttgtgttgg attttttaca gacatttctg ccaccccttg
    11761 cttttctttt tattccatct attatacttg tatctttcat tttttcaatt gttcctgtta
    11821 cctattcatg ctaaaataaa ggatagtttt caacttgaaa gagggtaaaa aatgaaattg
    11881 atcttattac ttattttagc atatctttta ggttctattc cttccggtgt ctggattggc
    11941 aaattgtttt tcaaaaaaga tatccgacaa cacgggagcg gtaatactgg aacgaccaat
    12001 acatttcgtg tattaggcaa aacagccggg attgttgtcc tgcttatgga tattttaaaa
    12061 ggtacattgg ctacttgttt gccgatgatc ttccaccttt ctgggattaa ccctctttgg
    12121 tttggggtat gcgccattct tggccataca tttccgattt ttgctgggtt caaaggagga
    12181 aaagctgttg cgacaagtgc aggaatgctt ttaggattta atcctgtatt ttttgtttat
    12241 tcttgtatca tctttatcgt tagtttattt tgtacaagta tggtgagttt gagcagtatg
    12301 atcagtgcag tattgatcac tttatctacg atcgtccttc cttatgtagc tcctgtgatt
    12361 ttggctaagc ccaattggtt actgacgatc attgcctttt tagtttcttc ctttatcttt
    12421 tatcggcata aagacaatat caagcgtatc agaaaccata ctgaaagtcg aatcccgttt
    12481 ggtttgaata gcagcaagaa aaaatcctga tactttttca ttccaaagag ataaaacggg
    12541 agtgtgacaa atcgatgtca cactccctta ttacttacag gatagctttt ttacacacct
    12601 tggtaaacgg tgttccctgc taacttctcg gtcacaacct cttacttcac tgtgatggaa
    12661 tattttgtct tgaagatttc atgggcacct agttgattga tccctacttt ttccatcaag
    12721 tttccactac tgttcaccgt gtcagcgatt ccgcaccacg gttcgataca aacgaaaggc
    12781 gcttcttttg gataaggaga ccagatccct acataaggca tatctttata gcttaaagtt
    12841 acgctatggg aagatttttc acttcgaatg gcgtacgcat ttagacccct tgtttcaaaa
    12901 accaaggcat cttgctcaaa aagttcacga ttcaaagcca gattcgtatt tgtctgacct
    12961 aacgtctttt gatccatatc gatgaatggg cctttcaatg gtaggcgtaa tcttgatttc
    13021 aatggagaaa atgccagata gaaatcttca aatgacaatc cttcttcaag aggtacattg
    13081 aaagcaggat gtccaccgat agaaaagaac atttcttttt caccggtatt tgccacttga
    13141 taatgaaccg tgatgccgtc tcctcctaat tcatagctga tcactaattc aaaatcaaat
    13201 ggatactttt ctaatgtctc cggcgtactc cttaagatga aactagccat ttcttgtcca
    13261 tgttcaatga cttcaaaatc catatcccga gcaaatccgt gttgccccat ttgatatgtt
    13321 ttcccttgat aaacatactg atcatctttt aaacgtccaa caaaagggaa taaaaccggt
    13381 gcatgtctgc cccaatacgc tggatcacct tgccagatat actctaaacc atgttctttt
    13441 gatttcagac tgatcaattc cgcaccgtgt tcagcgattt cggcgatcag ctggtcattt
    13501 tgaataataa cagtcattgc cattcctcca acttcgactt actttttaaa atgggaaggc
    13561 tggaacaaac atttctatct gccccagtcc ccttacttct aataactgtg ttttgctaac
    13621 tcgtctttaa aacgggaatt caatactttt aaataagttc ccttcatgcc taacgaacga
    13681 gattcaatga ttccagctga ttccaatttt cgtaaagcat tgacgatcac agaacgagtg
    13741 atgccgatac tatcggcaat attagaagca gtcagacgac cttcatctcc atccaatgct
    13801 tcaaagatag cttgtacagc cttcaattcg ctataagata atgtgttgat tgccatttgg
    13861 acagccgttg cactccgtac atctgcttcg atgcttcgag acttctgata aaggatctgc
    13921 ataccgacaa ccgtagctcc atattctgct aagactagat cgtcatctgc aaacgattgt
    13981 tccatacgag ataagataat cgtacccaaa cgttcgccag ctccaaagat cggaacgaca
    14041 gtcgtcagac caaatggata tttctctctt gactctaccg gaaatgcagt aatatcacta
    14101 gtaattggga tattagcttc agttttcatc aactgtgcca ctgtatcagt ataagattca
    14161 ggaaattgtt tctcaacaaa cattgttttg attcgttcat tgttcacatc gtgacgttca
    14221 ttataaccta gtaatactcc gtcacttcct atgatatagg tgttgctgtc aagaatacca
    14281 cctagtgtga cagccatttt atcgtaaggc aaatctgctt gcatgtcaaa agtattcttt
    14341 tgctgcaaaa gtgtgtttat cttgcgggtt ttcgttagca agtctgtcat tgtcgtcctc
    14401 cgatttttta taaaatatag cgacttaaat cttcatttgc cgcaattgag ccaagtttgt
    14461 catccacata tgcttctgtg atcgttattt ctcccatttg catatctggt gcttcaaata
    14521 atagatcttc tagtagtttt tccaaaatgg tatgcagtct gcgtgcgccg atattatctg
    14581 tgtcacggtt gacatcgaaa gcgatttgag cgatccgttc aattgcttct tttgtaaacg
    14641 taactgacac attttctgta ccgatcaacg cgacgtattg ttttactaaa gcattatttg
    14701 gttcggtcag aatcttcaca aaatcttcag ctgtcaaatc atctaattct acccgtatcg
    14761 ggaagcgtcc ttgaagttct ggaattagat cacttggttt agacaaatgg aaagctcctg
    14821 aagcaatgaa taggacgtga tctgtttgga tgatcccgta ttttgtgttt acttgtgaac
    14881 cttcgacaat tggcaatatg tctctttgga cgccttctct ggatacttct ccagattgct
    14941 gtgatttaga agtgatctta tcgatttcat cgataaaaat aattccactt tcttccgcta
    15001 atttgattgc ctcactatgg atatcagcat ccttgactaa ttttgctgat tcttccttga
    15061 tcaataactc taaagcttct ttgactgtta ccgtccgttc cacttttttc ttcggtgtca
    15121 aagcacctag agtttcattt aggtcgatcc ccatttgctc caatccattg ttcatcattg
    15181 gtgcaggttt cttttggtct tcaacttcaa tggttacttc acgactatcc agtaaaccct
    15241 tttgtagttg ttccaagact gtacggcgat tgattcgaat atcttctgtc acttcttctt
    15301 gcggttcagc ggtattttgc tgactagcat taaaaagatt catcatttgt tcaaattgat
    15361 tagtggattg tttctgttct tttttgatac caggtaccaa gatttttacc aaacgttttt
    15421 ctgcattttt tttagcactt gtgtatacac ggctgtactg ctctttttct acgatttgga
    15481 tcgcattttc tactaaatcc cgaaccattg attctacatc tcttccgaca taaccgactt
    15541 cagtaaattt tgttgcttcg actttgacaa atggtgcatt gacgattttt gccaaacgtc
    15601 gtgcgatttc tgttttaccg acccctgtag gtccgatcat cagcatattt tttggtgtaa
    15661 tatcttgttg cattttttca tctaactgca aacggcgata gcggttacgc aaagcaacgg
    15721 caacagattt ttttgctgcg tgctgtccta cgatatattc atctaattca cttacgattt
    15781 gtcttggtgt ttgttttaat tcatacatat ctactcacct cttacagttc ttctacgata
    15841 atattatgat tagtaaagat acagatatct gcggcaatat ttaacgcatt ttctgcaatt
    15901 tctttcgcac tcatgtcgtt ttgagcatag tttttcattg ctcgggctgc agccaacgca
    15961 aaattccctc ctgatccgat agctaagata ccatcatctg gtgtgataac ttctcctgta
    16021 cctgaaacaa gaagcatttc tttatcattc attacgatca gcatcgcttc aagtttttgc
    16081 atagattgct gtgtccgcca ttcttgagct aattcgacag ctgcgcgttg tagattcccg
    16141 ttatattcgt ttagtttgcc ttcaaatttt tcttctaaag taaacgcatc tgcaacactt
    16201 ccggcgaaac ctacaacaac ttctccattg tagatacgtc gtactttctt tgcagtgcct
    16261 ttcatgacaa cagattcacc cattgttact tggccatctc cagccatagc aaatttcccg
    16321 tctttttcga cggcacagat agtggttgaa tgaaatggtg attcgaccat gtttgtttcc
    16381 tcctaaattt gttcttttcc ttatttttac gctcttggat ggaaagaacg atagtttttt
    16441 tgtaaacttt cttttgttac atgggcatag atctgggtag tcgataaatt ggcatgtcct
    16501 agcaattctt ggactgtccg caaatctgcc ccattattta ataaatgagt agcaaatgta
    16561 tggcgcagca tgtgaggatg gatatcacta tccaaactgc tttttttgat catttgattc
    16621 aagacatatt caattccagt aggcgtgatt ggttttccat gatgattcac aaaaacgata
    16681 tcatgctctt cgtgatattt tgtcatcaat acctggcggc cttccctgaa atattcttgc
    16741 agagcatctt gagcaaaaga acctagagga gcatatctct ctttgttccc tttaccatgg
    16801 atcaacatga cctctgaatc aaaatcaatg tctgataccg acaaactgct acactcactt
    16861 aaacggatgc ctgacccgta aaggacttcc agtaaagcgc ggttcctcaa atccaaagga
    16921 ctatctcctg ctacgctgtc aaaaagtgct tgcatttcat tttcataaaa aaaacgtggc
    16981 agtctcaaat ttttcttttt gagatgaacg taagaaaatg gattttcttt tatcacttct
    17041 tctttgagaa gatactgata aaacgaacgt aggctggcta tcttgcgact gatagaattt
    17101 ctgctatagc taggacgatg atcagctaat gcactcaaat agacacgaac cgtaaaatat
    17161 tccgccttca gcagatcagc gtctccagat tcctccaaaa aatgtttgaa atggagaatg
    17221 tcttcttcat atgcttcttt cgttttttct gaatatcctc tttcgacaat caaataacgc
    17281 aaaaattttt gttcccacgt ggcttctgtc attttctcac cttccttttg tcccagagaa
    17341 taatttagca cagcgtttat aaaaagacaa ataaattgtc agaatttaac caatatttct
    17401 aagtttgttc acaattggcg caattcctca cattttgttt gagaaattct taagaaaaac
    17461 gcaagaaaaa gagcgagact atccgtctca ccctcttcat ggagtttcct tactcatttg
    17521 ggattgcagt tttttctaat tctgttttta ttgcctcatt agcttttaaa gctcgttgtg
    17581 caattgcttc atatcttgct tttttgtctc gaatgcgctc tggcagttca ggaaacagac
    17641 catagttggc attcatcggt tggaaatgtt ttccttccgc atgagtaatg tagtaagcca
    17701 tacttccaat agctgtttct tgtggaaact cgactggttc catttctttt gctaagcgtg
    17761 cggcgttgat tccagcaagt agtccgctcg ctgcactttc aacatagcct tctacaccag
    17821 tcatctgacc tgcaaagaac agatcttcgc gtttcgttga ttggtaagtc ggtttcagca
    17881 attctggcga attcataaag ctgtttcggt gcatcacacc atagcgaacg aattcagcat
    17941 tttctaaccc aggaatcatc cggaaaacac gtttttgctc gccccatttc aagtgagtct
    18001 gaaagcccac gatgttgtat aaagaagctg cagcattgtc ttgacgcagc tggataacag
    18061 cataaggacg tttgccagtt ttaggatctt ccaatccaac tggtttcata ggtccgaaaa
    18121 gcatcgtttt gaatcctcgc tgtgccatta cttcaattgg catacatcct tcaaaatatt
    18181 tttctttctc aaaagattta agttctgcca cttcggcatt gattagtgcc tcatggaaag
    18241 ctttgaattc ctcttctgtc atcggacagt tcaagtaagc tgcttctcct ttgtcatagc
    18301 gggacttcaa atatacttta tccatatcga tcgtagcttt gtctacgatt ggtgcagctg
    18361 catcatagaa ataaaaacca tctgaaccat tgaatgcttt gatgctttct gctaatggat
    18421 gagaagtcaa aggtcctgtt gctactacgg tgattccttc cggtatttca gtaatttctt
    18481 cgtttatcac tgttactaat ggatgctctt tgacttttcg ggtaattgtt tcagaaaaag
    18541 aatcgcggtc aacagctaag gcaccaccag ctggcactgc tgtttcatct gccgaagtga
    18601 ttactaccga attcaatcgg cgcatttctt ctttcaataa ccctactgca ttagccaaac
    18661 tgtttcctct taatgaattg gaacatacta gttcagcaaa attacttgtg tgatgcgcct
    18721 cagtcgattt gactggacgc atttcatata aacggacagg gactcctgct tgtgcgatct
    18781 gccaagcagc ttcacttccg gcaagccctg ctccaacaac atttaccatt ttcaatgaaa
    18841 gaacctcttt tctattattt ttggatgtct tcttcgtaat ctccatttac acagactact
    18901 tgtttaccgc ctttgacttt cttctcgacc agatatccgt cacattttgg acaatttctg
    18961 cccactggtt tatcccaaga agtgaattca cattctggga aacgggaaca accatagaat
    19021 agtctgtttt tcttagattt tcgttcaatg acttgtcctt catgacatac tgggcaagtc
    19081 acaccgattt cttttacgat tggtttcgta ttccgacaat caggaaaatt actgcaagca
    19141 taaaacttcc catatttccc taattttatc accatcggat gtccacagac atcacaatcg
    19201 aatcctgctg gttcatcttt gatttgaatt ttttcgattt tttcttccgc attcgtcagt
    19261 tctttttcaa acggtttata gaaacgatca accactttga cccacgcttc cgtgccttct
    19321 tcaattttat ccaaatctcc ttccatttct gctgtgaaat ggatatcgac gatttgaggg
    19381 aagaattcac agattaatga attgacaatt tctcctagtt cagtcggctc aaatcttttt
    19441 tgtgtcaatt ttacatagta gcgtctttgg atcgtatcaa gtgttggtgc ataagtggaa
    19501 ggtcgcccta ccccgttttc ttctagtgca cgaatcaacg tagcctcact aaaacgtgct
    19561 ggtggttgag taaaatgttg ttttggttca atatcaacag attggactac atcgccttct
    19621 tcaagttctg gaagaatatt ttctttctct tctttgccgt catctctacc ttccacatac
    19681 acttgcatga atcctttgaa tttgattttt gaaccgttcg caatgaagat gacaccattt
    19741 tgttctaatg tcactttcat tgtatctaaa atagctggtg tcatctgact agccacaaaa
    19801 cgtgaccaga tcaatgtata aagtttcaat tgatctttat ctaaatattg tttgagttca
    19861 tctggtgtac gaagcacact cgttggacgc acagcttcat gggcatcttg tgcgccttga
    19921 gcatttttga tctttcggtg atcatgtcta gaaaattctt ttccatacgt atcttcaata
    19981 aatgctgcgg cctctgcttt tgctgtatcg gcaatccggg tagaatcagt acgcatgtat
    20041 gtaatcaagc cgactgttcc ttgtttaccc aaagtgattc cttcatataa ttgctgtgct
    20101 accatcatgg tttttcttgt tcgaaagttt aacttacggg cagcttcttg ttgcaggcta
    20161 cttgtagtga aaggattcgc agggtttctt ttgcgctctt tcttttctac ttttttaact
    20221 tcgaagtctg ttccatcaat tctagcagtg acttctttta cactttgtgc atctggtaat
    20281 ttctttttct taccgtcgat tccccagaaa tttgctttga atttcttgcg agctttttta
    20341 aaattaccgt cgatgctcca atattcttct ggaacaaatt ctcggatctc tttttcacgg
    20401 tcaataataa tttttaaagc gacagattgg acacgtcctg cacttaaccc tttcttgacc
    20461 ttacgccaga gaataggact gatcgaataa ccaacgattc tgtctaaggt acgacgagct
    20521 tgctgtgcat ctactaaatc tacatcgatc gttcttggtt ccttaaaagc tgctttgact
    20581 gcgtcttttg tgatttcatt gaagaccacc cgattttttt ctttcagatc caacccaagt
    20641 aggtacgcta aatgccaagc aatcgcttct ccttctctat ccgggtcact tgccaagtaa
    20701 actttttgag ctttttttgc tgcggctttc aggcttttga tgacatcgcc ttttccgcgt
    20761 atagaaatat aatggggttc ataattgttt tcaaaatcga tacccatttt acttttcggt
    20821 aaatctctta tatggccaac actggccatt actttatagt ttcgtccaag atatttctca
    20881 attgtcttgg cttttgcagg tgattctaca atcactaaat atttatacgc cattactgaa
    20941 tggactcctt tccattgtcg tgttcctact attaaaagag gattcataca aatcgtagct
    21001 aatcatatcc attcagaaaa cgtttgtcaa gaaaagcaaa tgaaaagtat ggtgttattc
    21061 tttaaattct gccaagatat cttcacccga aaccgtgcaa actgcaccgt cttggatcaa
    21121 atgatgacat ccggtagaat gttctgttaa aatatttcct ggaacagaaa aaactgttct
    21181 tccaagatcc aatgcttgct gtgctgttat cagagagccg ctttttttct tggcttcaaa
    21241 gacacatgtt cctttagaca aagctgcgat aatccgattt ctcattggaa aacaatgctt
    21301 tttcgggccg gattgaggag gatattcact caaaagaaga tgatcatttg caatcacctc
    21361 ttgcaagtca cgattttcag caggatagac ttgctctatc cctgttccaa tgactgcgat
    21421 tgtccgtcca ccatgagaga ttgctgcttg gtgaccgtat gtatctatcc ctttcgcgca
    21481 gccactgacg atcacatatc cagcttctat cactggtttt agtaaataat aaactgtttt
    21541 tttggctaaa gcagaagtgt gtcttccacc aacaacgctt aacatgttac aagaaagtaa
    21601 agatagatca ccattataaa acagtacaat tggcggatag gtcaagtgaa gcagctgagg
    21661 gggatatttg ggatcaaagc aagtgataaa cgactgtttc gattggattt tgccccagtt
    21721 agcgtgaatt tcctgccaac ttttgcgaaa cagctcccgg tgtttggtta ttttacctat
    21781 cttaatgatg tcttcaaatc cgatttcttt ataaggatac tgcatcatcc agttagcaag
    21841 tgcccatttt ccttgactac tgattccttt tgtataagca agtttcaata aattttcttc
    21901 tatttgatac atcataaaac cgcctttcgt ctctctttct aataagatac gtaaaagcgg
    21961 ctgtttttta ttgtaaatat ttttttacag gtgcaaaact tttacgatgg attggcgtaa
    22021 ttccttgtcg ttcaagtcct tctaagtgtt cctttgttcc ataacctgca ttatgcgaga
    22081 aaccatatcc aggataaagc acgtcatatt ctttcatcaa ttggtctcga tatactttag
    22141 caatgatact tgcagcagca atcgacacag atcgtgcatc ccctttgatt attttttcct
    22201 gctgaatagg caaatccaag gtcatcgcat cgatcaacaa atagcttggt acaatggaca
    22261 atttttctac tgcttggatc atcgctaatt tacttgcttc ataaatattc acatcatcga
    22321 ttgtctgctg atcaacgatc ccgatcccga ttgccagggc tttttcttga acttgttgat
    22381 aaagaagttc tcgctttttc tctgataatt gtttggagtc attcagccct aatatgatct
    22441 catttttcgg caagataact gcagctgcca cgacaggtcc agctaatggt cctcttccta
    22501 cttcatcaat gccggcaatc cattggtgac cttgtgcgta cccttgcttt tcaaagcaaa
    22561 acatttcttc atatttttcc ttaattaatg cttccttttt caatcgtcgt tcaaaagaag
    22621 ccagtgcttg ctgcacgcct ttgcgtggat catttcttaa tgattctagt gtaggatcat
    22681 ctattctctg tatgcttttc aatcgttctt tgatactttg gatggattca gtcatcactt
    22741 ttttgctcca tttcttcgca acgatccagt gtgtatcgtc caagttttcc ttgacgaata
    22801 tcatgcacga ctaattcgct gccacgatca tagtcttctt tatacccacg ttttgctgta
    22861 atatccatca gtaattccgg tgccaatttt tcttcttgct cgattgataa accataacga
    22921 tcgctgattc ttcctgaata atatcgggca aaaaagtcta atccgtaaat cgctaaatca
    22981 tctaaatgga caagctggtc tttgatcgcg cctgtcagcg ctagtttttt gccgatttct
    23041 tcatcttcaa atttgggcca taaaatccct ggtgtgtcta acaattccaa ctcattacct
    23101 aaacgaagcc actgctgacc ttttgttacg ccaggcttat ttcctgtctg tgcaattttc
    23161 ttgcctacta gtcgattcat caacgtagat ttcccaacat ttggaatacc taagaccatt
    23221 gcccgaatag cgcgaggctt gatcccccga gcagcatctc ttgcccgttt ttctttcaat
    23281 gcttctttag ccgcaggtac tagaccttta attcctttac tttcttgtgc cgtgatagct
    23341 aatgcaagat ggccttgttt acgaaaatat tcttgccaca gctttgtctg ttgtggatct
    23401 gccagatccg ctttattcaa taaaacaagg cgaggttttt gctgaatgat ctgatctagc
    23461 attggattac gagaagataa aggtaatcgt gcgtctacca attcaaatac gatgtccaca
    23521 tatttcattt tttcagaaac ttctcgtcgg gcttttgcca tatgtcccgg aaaccattgg
    23581 atggtcattg ttctctccat tcctttcata ccggaatatt ccccggcttt acttgttttt
    23641 ttctttttca atataaattc acttttttat tataaagaaa ttccatctat tagttaagtg
    23701 aaagtgaacc agtcctcatt ctatcacatc ttcacttttt tgatgatgca aatttttatt
    23761 ttggtttcta tatatgaatc taaatttttg acagcgtttc ctaaataaga gatgatagac
    23821 gaacaaaaca gaaaggagac gaaacaaatg aatagtatgg tttttgttaa ttttcctgtt
    23881 gctgatgtca atcgttccgt tgctttttat gaaaagctgg gttttaaaaa aaatgaagaa
    23941 ttttctactg aagaagctgg ttcaatggta tgggatgaca ctttttggat aatgcttctc
    24001 tctcatgatt tttataaaaa atttataaaa gataaagaaa tagctgataa taaaaaaaca
    24061 agtggtgctt tgatttcgtt tagtgttgaa aatgcggatg aagtaaaaaa atttgctcaa
    24121 attgcaaaag aaaatggtgg tactttccac gaagtagata tgggaatgcc tgaagaagaa
    24181 atgttttcat tagaagtcca agatctagac ggaaatacat tagaacccgt atggatgaag
    24241 atgtaagcag ataactaatc tgacgttttt tataacaaaa aaacaaaggc ggatgatacg
    24301 ataagtattc tccgcctcta ttttttttat tcgtacaatc tcttttttac agtttataaa
    24361 agcagtcttc tcaccctttt tttaattgtt agatgccagt gtatgtaaat caccaatgtg
    24421 tcatgacaca ttggtgattt tatggtatcc ttttattatt aactgacaaa ggagagaata
    24481 ttttgaacac tttgattgca aaagacgttt ttagattgcc cgaaatgaat gcaagattgt
    24541 tgtcgacctc tgctctttta ttagcccttg atgtcatctt atacaagata gcgattggtc
    24601 ctgcttattt tcaaatcaca ttcggatttt taagtatggc gcttatgggt tatctatatg
    24661 gaccgatctg ggctggtttg cttgaaatgt tgtcgaacac tttaaatttc acgatatttg
    24721 gttcaggaac gtttcaagct gcttttctta tcacggcttt ccttggtgga tttattaatg
    24781 gcttattttt atatcataaa aagataaacc tgccttccgt catcgctgcc caattattga
    24841 ttatgatccc agtcagcctt tttatcaatt cttggctgat gtctgttttg tttggtgcag
    24901 atttcaaagt aatttttcta ggtagattca tcaggaatat gatccaaatc cctattcaaa
    24961 taatggttgt ttattcattt atcaaaactt tagaggctag aaaagtgttt aaacgagctg
    25021 tttgatttta cgtactaagg tagtaaactg attttggatt aaacaataaa gcgtgcagtc
    25081 tcaacagact gcacgcttta ttgtttctca ctggatccat tttatttcat caagtggata
    25141 atagacaaat cttgctttac ctagaatatc agttttattg atcgtgccaa acgaacgact
    25201 atcttttgaa actcttcgat tatctccaag tacaaaataa ttatcttccg gaagcttgga
    25261 ttgtcccgtt agctcttgta gtgtaaagtc cgtcgtatag gatgcatttg catgatctga
    25321 tttcaggttt ttagttagat aaggttcctt aactactttc ccattgatct tcaattgatc
    25381 attttggtag ctgacatttt ctcctggaag accgattact cgtttgatat aaatcgtgcc
    25441 atctgctaat tgaaaaacaa caatatcaaa tcgtctgatc tccgaaaatt tttccatcac
    25501 aaccatatca ccttgcttca acacgttttc cattgaattg ccttccactg gtaccgggat
    25561 gaataaaaat ccccgcaaca tgaacgcaag gaatacagaa acaagtaagt atttgaatac
    25621 aagccagaag cgattaatat agcgttgttt ttttgtcaat ctctatcaat ccatccctct
    25681 ttttttcatt ctttctttat tataagaaaa atatcaaaaa aatgcgacta gatagctgaa
    25741 ataaaaataa ttctttcaat taggctattg gacaaaaaac ttttttgatc ataaagtaca
    25801 aaactatctc ttatatgtca tgattatttt ctaaatattt ttcccatgct tcgtcaaaga
    25861 tatccatgga cggcaaatag tcgacattca gttccagata attagaaatt tcttcgtagc
    25921 tagagctgtg cttaggaaat tggatgtctt taccggcttc tgtagcgaaa acagataaag
    25981 catcatgtgt gccaccgcct cgcagtgtca ttaaataatg aaaaaagctt cgattcaccc
    26041 ttcgttcctc caattttctt cgataaaggc agcccggcgt gcatggtttc ttttaaaatt
    26101 ttcaggatgc tttttataat actcttgatg gtcttcttct gctagataaa atggttgggc
    26161 tggttcgatt tttgtaacga ttggctgttc gaaacgtcca ctttcagcta actgtttctt
    26221 acttttttca gcaatgatcc gctgttcttc tgtcaaataa aagatcaccg ggcgataatt
    26281 gtcaccgcga tcctcgaatt gtccgaaagc gtcagttgga tcagtttgct gccagtaaag
    26341 ttctactaat tgttcatatg aaatcaatgt tggatcatag ataatctcca ctgcttctgt
    26401 atgtccagtc gtttggcttt ttacttgttc ataagtcgga tctggtacat gccctccagt
    26461 atatcctgac atcactgtat ggataccagg aagcgtgtcg aaaggctgga tcatacacca
    26521 gaagcagcct cctgcaaata ttgctcgttc cattatgatt ccccctctaa tactactcat
    26581 ccgatgattt tttattttct tttgtcttgc tttcagtact gctggtgcta gttttgttgg
    26641 tatttgagtt gtttgaagca tcagaggtat cttttggcaa atatagacta aaccgaatgt
    26701 catcatttac taagtcgatt ttatctgctt taatatacat cccgttttgc attcggaatt
    26761 gatccaacct aagcatgata gtcagatcat ctgtattgac ttcgatccaa tctggtaatt
    26821 tatagtttct cttgatcata ttcatcacat ctttgattgg caaactcaat gttccgatag
    26881 aaagacttct agcttttagt tggacatttc cgttttccat cacatacgga tcaaaatata
    26941 aatagaaatc gataggaaaa cctagtactt cgaaagtacc attgagtagt gcttcatttt
    27001 ccaaataaaa tttatattca atatcactat ctttttgata ctcactaagg aaaaagctga
    27061 tgatttgatt gactttttct ttatttgaat tgatcgttaa gacaggatct ccttgtcttt
    27121 ccgtaagagc tggtatttct gggagttctg gttcccgatt agcaaaaata cggctgccta
    27181 aaaatacggt cgaaccaagt actaccccaa gaaggataag gaaggcgaat ttccagtaat
    27241 ttgttttctt tgttttgttt gaagatacgt cagatcttgt tttcatgttt attgatcatc
    27301 ctccttctct aaccattggt tttgtgtctc gatcagctga tctctcactg catttgccat
    27361 caattgataa cctaaattat ttggatggaa atgatcttct tcatagagca cattattttt
    27421 gatgccattt agattttctg tcgtgctagt tgtttgatct gttccttctt ctgtgacacc
    27481 tacttcctga ttcaatcctt gataaagcaa atcattgatt gggataaagt agcagttcgg
    27541 catttttttt gtcaccgttt ctgttccttt gttccaattg ttaacaattg tctgcatgtc
    27601 agtgatctca ggaaagttca aataaaatgg attgtagatg cctaaaatgt aaataggtgc
    27661 ggttttattg agggatcgaa attcttccaa tagttctgtg acattttcct gatagttttt
    27721 cagtggcttt ttaaaggaat tgaccgttaa cccgaataaa ttattttgga taactttcat
    27781 caaatcatta ccgccaacag tcagtgtgat aaaatcagct gttttgatat tgtttcgaat
    27841 ttcttctttt gtttttactc gctttaagat ctggtcactt cgttcaccat ttacaccata
    27901 gttttctgtt tcaaccgcgg tcaaattata acggtctttt agatcatttg cgacaagggg
    27961 aacgaatcct ccttgcttgg tttgatctcc tactccttca gtcagcgaat ctccgatagc
    28021 tatgtagtga agtttttctt ttttatttgg atcttctctc acagtagtta ctggtttcat
    28081 tagtgggcca gctttcggta tagaagcagc taaacctgca tagatgatga tcgcaattaa
    28141 taatgggaac aaaacgagga tcaaatgtcg tttcgttaat ttcatttttt cacctcttgt
    28201 attcgattgg ttctaatgtt atcaaaaatt ttatcaaaaa ggtagtcttt atgaaaaaag
    28261 aaaacaatta ttttcctaat tttataaaga aaaagagaat aagctgcaag cctttcaggc
    28321 tgagggctta tcctcttttc gactaatcta cataatacat gattgcaaac gcgtctttac
    28381 cagtatgcgt agcgataacc ggattcgtat gcagtacagg gatatgcatg tccgggaata
    28441 attcttgcaa accctctttg aattcttgcg ccaattcttt tgcatccgca tgtgaaatac
    28501 cgatttgtcg cacatttggc aattctttca gttcattttt caaattatta aaccatttat
    28561 cgaacgtttt ctttcctcgc cccttagtta cagggattaa ttcagaatgg gcgaaattca
    28621 tcaccacgcg tatgttcaaa acactagaca gcaaaccagt tgttcggctg attcttccac
    28681 cttttaccaa gttatctagt gtggatattc cgatgtataa ttttgtattg tcttgtactt
    28741 ttttgacttc ttcaaggatt ttctctactg ttttaccttc ttttgctaat tcagctgctc
    28801 gaattacctg aaaagataat ccttggtcag tggtatcact gtcgataacc gttactttcg
    28861 tttttgttag attactggct tgtcttgctg cttctactgt tccactcaag ccttttgtca
    28921 tatggatcga aatcacttca ctgccgtctt ctcctagctc atcatacatc gagatgaatt
    28981 ctccaattgg tggttggctt gttttgggca gattttttgc tttcgccatc atttccataa
    29041 atctttcgcc gtctagttgg tcatcatcgg gatatactgt tccgtcgatc atgatggata
    29101 gcggcatgac acgaatgcct aaagcatcgc gtacattttt ttccatcgta catgaagaat
    29161 ctgttacgat ttttacattt gtcattattt tctctctctc tttctttaac aagatgcctt
    29221 attcatgtta aactacatgt aagacaattt ctatttttag tataacagac aaataatgat
    29281 ttttcattaa ttacacctag taaaggagcc gttttcatgg aaaaaacgaa atttaatcga
    29341 acgtatgaaa tattaaatga agtgtttaat gcagtcaccc atggaattgg gactggattg
    29401 agtatcgcgg gactagtggt tcttttagta aaaggtgccc acttgcattc ctccctccat
    29461 attgtctctt atgctatcta tggatcgatg atgattcttt tatttttaac gtctactttg
    29521 tttcattcat tgatttttac aaaagcaaaa aaggtgttcc aagtattcga tcatagttcg
    29581 atttttcttt tgattgcagg aagttatact cctttttgtt tgctgagtat ccgaggatgg
    29641 ctaggttgga ctttatttgt attaatctgg ttgtttgcga ttcttggcat tatatataaa
    29701 gcgttgacgt tgcataaacg ggagagagta tcaaaagtat ctacgatcat ctatattttt
    29761 atggggtggc tctgtgtcgt ggctgctgtt cctctttatc actctcttgg aatgactggt
    29821 gttattttga tggtagcagg cgggctttct tacacgatag gcgcattctt ttatagcttg
    29881 aattctatcc gctatatgca tgttgtttgg catttgtttg ttatgttagg tgcaggtttt
    29941 atgtacttct ctgttttatt ctttacttaa taaaaaaaat aaagctatgg atgggacagc
    30001 tctaaataac agctagtcta tccatagctt ttttatatcg tcaattcgcc tgtcccgctt
    30061 tttacaggat tctcattacg ctgatacgtt tcaaattgat gcggataacg atttttttca
    30121 tccatttctc cttgactagt attgatcagt gaccaatcag accagtcaac aggtggaaaa
    30181 taagcatctc catcgaatac atgatggatg actgtacggt ataatatcgt acaaaatggc
    30241 agaaattctt gataaacagc tgaccctcca gcgatgaaaa agataccgtc tgtttctttt
    30301 tccagagcaa gcacgtcttc cacactatgc atgacatgca cgccttctgc tttataggaa
    30361 gaatctctcg tcaaaacaat cgtctgacga ttcggcaacg gacggctacc catcccttcg
    30421 aatgtttttc gtcccatcac tagtgtattt gcttctgtca tttgtttgaa gaatttcaga
    30481 tcatttggca aacgccaagg aagacggtct tcttttccaa tcaatccgtt ttcatcctgt
    30541 gcccaaattg cagctaacat tttctcatcc tttctgattc taaacagcga taggagcttt
    30601 gatcgccgga tgtgggtcat atccttctaa ttggatatct tccatctcaa aatcaaatac
    30661 tgactgcttt tcctgattca aaactaaagt cggaaatgcg cgtatttctc ggctcaattg
    30721 ttctttcatt tgttcgatat gattgatata aagatgagca tctcctaatg tgtgaacaaa
    30781 ttcgccaacc gctaaacctg tttcatgtgc aattaggtga gtcagcaacg cataacttgc
    30841 aatgttgaac ggaacgccta gaaacacatc cccactgcgt tgatacaatt ggcagcttag
    30901 ccgtccctct tgaacataaa attgaaacat cgtatgacaa ggaggcaaag ccatgctagg
    30961 aacatcttcc ggattccatg cagaaacgat caatcttcta gaatcaggag tctttttgat
    31021 tgcttcgatc acttgtttga tttgatcgat aaagccaccg tcttttgttt cccagtgacg
    31081 ccattgatag ccgtagatgt gtccaagatc gccgtatgtt tcagcaaagg aatcatcggt
    31141 taggatcttc tcgcaaaaaa gcttgctctg ctttttatat tcttcattga attcaggatc
    31201 tgtcaaacat ctatggccaa aatcagtcat atctggtcca ttatagtctg gacttttgat
    31261 gtagcgttca aatgcccatt catcccaaat atggttttta ttttgtaata gatagcggat
    31321 attcgtatct ccttttaaaa accaaagcaa ttcgcttttg atcaatccaa aaggtactcg
    31381 tttcgttgtt aacaaaggaa accctttttg cagatcaaac cgcatttggt atccgaataa
    31441 gcttaatgtg cctgtgcctg ttctgtcttc tttacgatgc ccttcttcca acaattttct
    31501 tcctaagtca agatacgctt gttccatcct ttttcctcct aatcttctac ccattcactc
    31561 aagtattccc accgtgccat tttttcttca agttgtgctt ctgtttcaga tacgtcgttt
    31621 tgcaattctt gtaatcgagt gaaattatct ccttgatgat tcatctcttc ttggagcaat
    31681 gagatttttt cttcaagttc tgcaatctca tcttcaatcg tttcccattc tttttgttcc
    31741 atgtaagata atttcttctt ttcagcaggt tccttgctct gtatttttgg tttcgctttt
    31801 tcagcaggct gctgggtctc tttttgcttc agtaaataac tggacatgtt tccgtaataa
    31861 agttcttgtt ttccgtttcc ttggaatact aataacttat ctgctacctt gtccaagaaa
    31921 taacggtcat gggaaacagt gatcacagca cctggaaaac ttctaaaata atcttccaaa
    31981 atcgtcaatg ttgctatgtc caaatcattg gtcggttcat ccaataataa aacattaggt
    32041 tgctgaatca gcagtttcaa caaaaataac cggcgttttt ctccgcctga aagtttgcct
    32101 atcagtgtcc catgcataaa tcttggaaat aaaaatcgtt caagtaattc tgctactccg
    32161 atttgactgc catctgctgt ttttgcttct tcagcagctt cttgtaagta agcaatcatc
    32221 cgtttatctg gtgccatttc ttcattttct tgtgtataat acgcaagatg aaccgtttca
    32281 ccgatagaca ttgtaccgct atccagggga attctgccag ctagaatatt aagcaatgtc
    32341 gattttcccg caccattttt accagtaata cctagtcttt cccgagattg aatcaataaa
    32401 tctaaatgtt ctagcagtgt ttggtcattt atacggtaac tgccatcttt gatttccagt
    32461 acttttttcc ctaaacgttg tgtcgctagg ttcagctcta aatcatcttc ttggtttact
    32521 tggtacaagt tctcctttag atcttcgaaa cggttgatcc gtgcttgctg cttggtgcca
    32581 cgtgcctgta cgccagccct catccaagct aattcctgtt tataaagccg cttgcgcttt
    32641 tcttcttgtt ctacttctac acgatcacgt tctgcttttt ctaagacgta agcttcataa
    32701 tttcctttat actcgtataa ttttccaaat gaaagttcga agattcggtt tgctactcta
    32761 tctagaaaat aacgatcgtg ggtaaccatc aatagtgcgc cacggtattg tttcaaatag
    32821 ttttccaacc actcgatcgc ttcataatct aaatggtttg ttggttcgtc tagtaatagt
    32881 aaatcgggtt catcaatcaa cacttgcgcc agactaactc gttttttctg accacctgat
    32941 aaagtactga tttttttatc taattcactg attcctaatt tttgcaaaat aatctttgcg
    33001 ttggtatctg tagtccaagc atcttcttta ttcatttgtt cttctgcttt agcataacgt
    33061 ttttgcacat cagggtcttc tccgttttcc cctagttcga tcaatgcttc ctcatatgct
    33121 cgaacagttt ggatcaaggg gctgtttcct tgaaagactg cttggagaac agttaattca
    33181 tcggaaaatt cagctgcttg agaaagataa ccaatacggt aatcagttgg ataaaacact
    33241 gtttgccgat caccgtcgct gctatcgata ccggcaataa tatttaagag acttgtttta
    33301 cccgtcccgt tcgtcccaat caacccgata cggtcttttt cgtggaccaa aaaagaaatc
    33361 tggtcaaaca aatctttttc accataagtt tttttgaggt cagttacttt taattctttc
    33421 aacatgttcc ctccttatct tggatattgt atcataaaaa tagtgaactt caggaaaatt
    33481 tttttggtat ttgttctttt tgtgttctca aaaaaagaac aggaccgatt cgatcctgtt
    33541 ctgatatgtt ttgtttttta taaaacttga aaatttttac ctttttattt tattggactc
    33601 gttttttttg actttgctcg aaacgcaaat cggaaagaaa agttatcaga tggtttgttc
    33661 atcgtataag attctaacaa attgatattc ctgcgatagc tgttcgcaga aaacgtcgtg
    33721 attttttcag caacttcgta ttcatcaatg atttttcttt caagataata acctcttagc
    33781 atttttttcg taaacagact atcttgctgg atcatccacg aagaaatcaa attccccgag
    33841 ttcattttta ttccttcaag tttgcgttgc tggataaaaa attgaaccat cgtttcatca
    33901 taaatatcag tcaagttatc taaactttca ataatcaact gagtattttt ttgatatgtc
    33961 tctctgattt cttggatatc ttcatcacta ataaatgtct gacgatcagc atatcttctt
    34021 tgccaaaaca ttttcggatg gagaacgatt cgcattaacc tacggacaac aatgaaccaa
    34081 aagccgataa aagacaaaat ctggcttgtg atagaatgtt cgaatcgaga aagaaaacgg
    34141 gagtaaaaac gataaccatt agaagaaagc ttgcccgaac gataactttc atccaatcca
    34201 tcccgttcga tcgacaaaat cagtgcttgt atttcttgta cttcttgctt ttcatcatcg
    34261 gttaaatctt ccaagtacag atcacgtatc cgttcttgat aattttctat cactgcttca
    34321 gtggctaaaa attcttttgt attacgatca atttccttta tttcttttcg caaaacttca
    34381 acgacctctt ctaaaatcat caaagcattc aaatccgtgc ttttcgcttc ctctgtttcc
    34441 gtcagcatag gcaagacaat gatccctatg accaaagtca ctaagatgac acccgccgtc
    34501 agaaacaaaa gcaaagaccg ctggtaaaac atcatattat tgattgaaaa tggcaaaata
    34561 aaaatcgtag ccaaactgac tgttcccttt acaccgccaa atgttaagat caagatctcg
    34621 ttcaactgtt ttttgaactt tttactgcca tctttgaata cataaaaaag actgatagaa
    34681 atgaatctga tcacaaaaag catgacagag attaatacga taatcgctaa taaaagaccg
    34741 tttggatata aaccgtctcc ccatactggc gagaacactt gcgtcaattc gattcctaaa
    34801 aatataaata ctaatgcatt cagtgtaaaa gcaatagttg tccatgtact gtgggacaag
    34861 ctcgacaatt ctgcatcaaa cacagagatt ttcctaaaac cagaagcttg gagaatacct
    34921 gctgccactg cagcaatgat tccagataca tcaaatgcct ctgccaatac ataggctaga
    34981 aaaggaagca aaagttcgat cagcagatac cctgtgacat cttgtgctga tgctttttcg
    35041 atcaaatgaa ttattttttg tttgaaccag acaagcaaaa agccgatcaa agctccccca
    35101 atactagaaa ctactagact catccccgcg tttactgctg aaaaagatcc ggtgatcagc
    35161 gcaccaagag caaactggaa ggcagtaaca ccagatgcat cattcaaaag accttctcct
    35221 tccaaaatat gcatcgcctt tttagggata ttcactcgtc cagaaagaga actgactgct
    35281 accgcatctg tcggacctaa cgctgctcca aaagcaaagc aggcagcaag tgggacactt
    35341 ggcaacagga atgataacgt cgcacctacc ccgacaaggg tcaaaatgac tcctccaaat
    35401 gccaagaaca aaattgtccc gaaatttttg agaatcgaag aaatatctgc tttttctcct
    35461 tctctaaata acaatggtgc aataatcata actaagaaca attcaggttc aaaatcgatt
    35521 gattgtcccc actctgtcag acctaatacc actccaagga aaatttggat cagtggagct
    35581 ggaatcattg gcaaaatgcg gctagctatg ttcgataagg tgatcgttac tgcaaataca
    35641 ataactaact cagcaaattc catcgtccat cccctttatt ttttattttg tgtaagcatt
    35701 tttaaaaatt gttctttttt ctttaaaatt ttggtttcga ctacttcaac ctgtgtttgg
    35761 ttcccctcta attcacactg tacacgtttt gactccaaac gaagcagatc atgtttcaga
    35821 cgttccaact gacgatacat caatatattt cttttacgca acttacaaag tatttgacgg
    35881 cggtcatcca tcgaaagggc gccatagcga tcatgcaaca gttttttttg ttgttcaaaa
    35941 ctctctctca ttttccagat ctcctataca caaacttcca tcattatagc aaagttcttt
    36001 ttggatgaaa aatatttcat tgtttcatcg tttattgcta gttagaaatt tccataaatt
    36061 tttcaacgcc tcattctatt ccaaatagta tcagatatca agtaaaactt aagaaagata
    36121 aaagtttctg acaaaaggat gtttactttc ccattcctgc ctttcttgtt cttttatcac
    36181 aacaaaaaaa tggggctgcg acaaaaatct tgtcacagtc ccttattctg aataaacggt
    36241 gtggaagaga cagcctatca gtagtaataa gacacatctc tttcacagcc tcatttttgc
    36301 gtaaaaatcg atttattcga aaaattcccc gtatacatct tctacacgtt gatctggatt
    36361 gatcacgata tacaagttac ctgttttttc gctgattttt gttgattgat attgattgtc
    36421 taatccttct gttcctttat ctagatatgg gaagtacaca agatcaggtg tgccatttcg
    36481 gtaaaggatt tccattcgct cgatatcttc gtatttcaat gcgcgattaa acatgcccat
    36541 ttccagtccg cctaggttga tatcatgtgt cgcaactgcg tccccttcat ggaagatttc
    36601 gattttaaat ccagcacacg gatggatttc aacaaattca ctgccattga ttcgtccaaa
    36661 acttgttgtg atttgtttga tccaaagatc gccgatatga cggcggttga tcgtccaggt
    36721 ttctccattt ctaaaataaa attttagcgc tgtcatttct ttactcatta aaaagactcc
    36781 tttatgatgt aatttattct tgtactacct cagtttagca caaatttatg taagtggtca
    36841 catcttatgt tttttcttct aatgacgtat ctgcccactt taagtagcca acaaagctga
    36901 gcagatacag tctagtgttt tttaaatcaa tttttctttc gataaaaaat ccaaaacctg
    36961 ttttttatca ttttttattc tttgttctac gacggctttt tcagctaatt gcaaagcttc
    37021 actaacccac ttaccaggtt tttttccagt ttcttctagc aagattttac catctactgc
    37081 taattcgctt aatgacttga taggtaaacg atcataggat tctcctacgg catccatctg
    37141 aggtgtttcg tcaaaataga tcatcaattc ttctacttgc agtgcatgtt ttttgcctaa
    37201 gaagtacaat tcgaatggtt ccaaagctcc agtttctcgt tgtcgtagtc cttggacaag
    37261 tgcttgaacg tctttaatca tttggttaga acacttccac gatttcaaaa aggaacgaat
    37321 ctcattttct tttagatcca gttggctgat caataatgcc catgcttggc tctcttcttt
    37381 caatgggacg gatggtaatt cagcaaaacg taaaagtgct tcgccttctt ctttcaaacc
    37441 gggacagcaa atgtagcact ctgtttcaat aaatgcatgg attcccgcgg cccgttcatt
    37501 tcccaagagc atcttgatga attccacatt tatacgttct acagatattt tacctaatag
    37561 ctcgtggttt tctttgatgg cgtagaaggt ttcttcctct aatttgaacc ctagttgact
    37621 gacaaaacgc aacccgcgca tcattcgtaa ggcatcttca tggaaccgct catgaggatt
    37681 gcctacagct cgcaagatct gttcttctag atcttgcaga ccatcgaaca aatcgatgat
    37741 ttctccatct tctttcaaag caaacgcatt gatcgtaaaa tctcttcttt tcaaatcttc
    37801 tgccaacgaa cgaacgaatt cgacatgatc tggtctacgg tagtcctgat aagttgattc
    37861 cgtccgaaat gtggtgatct catattgttc atcattttct aataccaaca cagttccatg
    37921 ttcgatccca atatctatcg tttttggaaa aagctgtttg atttcttctg gaaatgcgct
    37981 tgttgcgata tccacatcat ggattggctg gttcagcaag atatcacgaa cacttccacc
    38041 tacaaaataa gcttcatatc ctgcattctc taattttttc agaactggaa ttgccttttg
    38101 gtattccaga ggtaattgtt ttaatttcat ttttccaacc tttcctgcgt gtttcttgtc
    38161 ctgagataaa gactcagttg catggtgact gtttgacaag tccgtttcag agtcttcaag
    38221 gccaggacca cttcatcttg tttactcgtc cttacgctca aagcgattat ggtctctttg
    38281 attgaatttt tccatgtttt tttcaaaaac ttcagttaag tcgatattca atgaattagc
    38341 catgatcatc gtaacgaata aaacatcacc aagttcttct gctaccgagt taactggttc
    38401 agaagccttt ttgctttttt ctccataagt gtggttgact tctcttgcca attcacctac
    38461 ttcttcagtt aatcgagcca tctgagctaa tggagagaaa taccccacct tgaattgctg
    38521 gatatattca tccacttcct gctgcatact gtataaagaa cgctttttat ccatcttttt
    38581 ccctccaaaa ctatcttacc aaaactttaa cgcaaatcct agataagagc aattgagaaa
    38641 atcgaatttc catgctacat ttaggaagga atttctaaat ggaaattctg tgaaaataca
    38701 taattgaaag ctggaattca tatggtcaaa cgaaaaacag taaaagaagt tctttttatc
    38761 attataggaa caagtattta tgcatttggt cttgtttact tgaacatcgc taatcattta
    38821 gcagaagggg gagtctcagg gatcaccttg atcttaagag ctttatttgg tattgatcca
    38881 gcttatacga ctttattaat caacatccca ttgattttac taggaggaaa gatcctcggt
    38941 aaacgttcct tggcctatac tgttctaggt acactttctt tgtcattctt tttatggatc
    39001 tggcaaaggg ttccactaga aatcaattta cagcatgatc tactgatcgt ttctctactt
    39061 gcgggtttag ttgcaggtgt aggaagcggg atcgtctacc gcatgggtgg aacaacaggc
    39121 ggcagtgata ttatcgctcg gattttagaa caaaattacg gcatcactat ggggcgttcc
    39181 ctattggcat ttgatatcat cgtcttactt gcttcattaa cctatatcga cttgaaacgt
    39241 atgatgtata cgttgattgt ttcgtatgtt ttcagtcgtg tgattgattc gatcttagat
    39301 ggcggatatt ctgcaaaagg tctacttgtc gtctccaaca aaagcgaaga gattgcacca
    39361 ctactgatga ccggtctgca aagaggtgtg actttccttt ctggagaagg cgcattctct
    39421 ggtaattcaa aaaaaatcat ctatatggtc gttagctcta gtgaattaac tgaagctaag
    39481 aggatcgtcc acgagattga cgaaaaagct ttcctttcaa tcatcaatgt gcatgaagtg
    39541 gaagggcaag gctttactta tttgaagcca cagacaagat tactgaaaaa actataagaa
    39601 aaaagacaaa gataggctat gaggaaagat tccacatcca gctgaccttt gtctttttta
    39661 gtactatact atttttaact aattgttatg ttcacttacc cggttacttt tcttttgtgt
    39721 ctgcaaaagg atctttcggt taacttcatc agataatttc aaaaaagcat ccctatctcc
    39781 atcttctagt gcttgatcga tttgagcata aagcattttt agttttgcct cttcttcttt
    39841 ttcttgaaaa tatcgttcaa ttaattcagt atcttcttca ctgactgaag aatcccaaga
    39901 agaaaatgga ttgtcttcta agatagatag atattctctt gtctgccaag cattttcgaa
    39961 tatacattcc acatataaag gctttttcca attcaaacgg atttcatgaa aaatctggtc
    40021 tgtgtccgta aattcttttc cctgcaaaaa caatttcatg ggttcccctt caacagatag
    40081 atcactgatc tgcagtcctc gatccgcaaa attcgcccct tcaacaaagt ggacattgct
    40141 taaaatcgct tcatgattgg caagataatt caagatccaa aaaacttctc ttcggctaaa
    40201 agatacatga ttgaccatcc acgttaaaaa attcttcttt tcccgtacat caattagcat
    40261 ttttctcacc tctctgttag gtcatctaat aatgacagtg cctccatatc tcctggttca
    40321 agtgttaaat aataagtcaa taattcttgc gctcttttca gctgcccttc ttctcttaag
    40381 aaaaaggcat actctttcaa gaaatctggt tcatgtgata gttcatgata agcctgttca
    40441 tagtgtacag ctgcaacggt aaagtcttct aattcattat aagcatgggc taggttccat
    40501 tctgcataag gattagcagt ttcttccatc tgattgatca cttgtatcac atcttcatat
    40561 ctttcatcat ccaagtaaag attacttaaa gtaagtaatg tttcatcttg tttgtctcct
    40621 acttctaaag ctttcagtaa aagttcttct gctcgctgtc tatcatgcag acggaaagca
    40681 ttttccgagg ccaggtgata aagttcgact tggtaaggat tttctttgat tccttcttct
    40741 aacactgttt gtgcttcttc aatcatttct tcttcttgaa gagcttgtcc tagatataga
    40801 tataacgatt gataatgcgg attaacttct cgaagttctt ccagatagtt gattgcctgc
    40861 tgattttctt tcaattgaag attcgtaaaa gctagctgga acagtcgatc atctgtcctt
    40921 tcttctttca gtgcttcttc taaaggtacg atagctgctt cgaattctcc ttgcatactt
    40981 aaggattgtc ctaaacgttc ctgcatcgag attcctgaaa tttcatttat cccgcttgcc
    41041 aacaatgttt cataaaccac tgctgcctct ttgaaacgat caactgaaaa atacaattca
    41101 gctagcgcaa actggatcaa tggttcatca ggaagcaacc cagctgcttc tttcaatttt
    41161 gcttcactca cttcagggat tccgataact tggtacaaat cagctatcgc caataagctt
    41221 tgcggatagt aatcactttg cttatccact ttttccaggt attcaaaagc taagtcgatc
    41281 tcattatctt cgattgcaat ttctgctaac gggatgttca gtccgtcatt ttctgggtat
    41341 tggttgatca gttgttcaaa aatctgtttt gcttcgatca aaaagccaat agccagcaat
    41401 tcttcgccta gttcagctaa tatatctgct gggtcttctt ttaatgcttt ttctaaaaag
    41461 atttgtgctt gtgacaagtc ttcatctgat aaagcttgca acattttttc gctatttgta
    41521 ttcatttcaa ttccctctta tctcactgac gatttgttga aataccgtca ttgttctcaa
    41581 taattctttt tcgtttggat gttggtatcc gtctattata cctatttttt ctaaaacaag
    41641 tatttttttg cttttttctg ccaaaaaaag aactttttcc aaatactcca tctttgaaat
    41701 ctgttccagc cagtggattg gatagccaag caagtccagc cacacaaaaa agttttttag
    41761 ttggaaatca aaaggatatg cttggagatt ccacacgaaa tgaaacatca ttcctaaaaa
    41821 ttttttcata ctagcagaaa gtagatggcc attttcggtc agataaaaag cttgttcaaa
    41881 cagtttacca taatttgaca ggttttctgc ctctaattga taaaaataga tcatttcttc
    41941 gatcatcccg gcaaacggcc gagtcgttaa ttgcttttta tttggatagt ttttatacag
    42001 attttgcaaa aaacgatgat cacaaatcat tccgcaacag ataaaagtca aaaagtcaat
    42061 ctgctttccg tccacctgcc gctcgctgat tgtttgatca taaacgatgc gttctggcag
    42121 attgatggtt tgcagcacta cacgatttgg tagctgcaaa atcgtccgct gcggtgttaa
    42181 tgctcggctt agagaacgga tcgatacagg gatcatccat agtgatgaat caagggttgt
    42241 atgtttttga aaaaaacctg ccagatccat gatcccttca tttccaaatc cgatgataaa
    42301 catggattga ttttcgggat agcgtttgac gaaatcaagc aggttcttta attctgttaa
    42361 atgattgcat tgagtgttgg cacaaatata ccagtcattg tttgcttgat ttgcaaataa
    42421 cgggttgatc ttatctgcaa acaaatcata atagcgctga ttcgttaaaa ataagattgg
    42481 ctgatcaatg gaaagttctc ttagctgact gactagcgtt tctccgtaaa acactttcgt
    42541 caacgcatct tctacttgat aatcaagttc catacgcatc cctcctcttc ttatttttat
    42601 tttaccatta gtaagcaaat tctagaaaag ataagagcaa agctcacctc ataacttgca
    42661 aataaataaa aattaaataa aatacatgtg ttttctatgt atgacatacg gattcggaat
    42721 ctctgtctct tatttattcc ttttctgcta ggaaataaaa agaaaagcac ttgtagccct
    42781 agagccacaa gcgctttttc aactgtctta tttaacagcg tcttttaatg ctttacctgg
    42841 tttgaatgca ggtactttgc tagctggaat ttcgatttct tcaccagttt gtgggttacg
    42901 acctttgcga gctgcgcgtt cgcgaacttc aaagttacca aaaccgatta attgaacttt
    42961 ttcgccgtta gctaaagcat cttgaatagt tgaaaataca gcatctactg ctgcagttgc
    43021 atctttttta gttaaatcag tagctgaagc aactttttcg attaattctg ctttgtttgc
    43081 catggataga ttcacctcct ccacaagaga tatgatagaa atatcattta gttaaaactt
    43141 tctgagtggt ccagaagttt aaaaagaaat attgattaac ttcaatattc tgttatcacg
    43201 atatcatatg aaccgcttaa tagcaaggtt tttaccaatt ttcacatgct ttttttagtc
    43261 atagaaacgt aacaaacgaa actgtctttt tcttttgaaa aaaaaacgtc gatatggtaa
    43321 aatgcctatt ttctcctacg agggataatt cggatggacg tcccttcaaa agtaaatgct
    43381 tttctgattt gattttctaa gaaacgttca taagaaaaat gcatcaattc ctcttcattg
    43441 acaaaaacga caaatgtagg tggtttgaca gcgacttgtg tagcataaaa aatcttcagg
    43501 cgttttcctt tatctgttgg tgttggattg attgctacag catccataat gatatcattc
    43561 aacacagccg aagagatacg aagattctgg ttcatactta cttgttcgat catttcaggc
    43621 agacgttcca accgttgttt agtcactgca gaaacaaaaa cgattggagc gtaatcaaga
    43681 taccggaatt catcacgaat ctcttgttcg aaatcacgca ttgtattggt ttctttcttt
    43741 acaagatccc atttattcac gacgataata attccacgac cagcttcatg ggcgtaaccc
    43801 gctactcgct tgtcttgttc ccgaatccct tcttcagcat tcagtaccat caaaacgata
    43861 tcagaacggt caatggcacg cattgcacgc atcacgctgt atttttcagt attttcgtat
    43921 acttttccgc gtttacgcat accagctgta tctatcatta agaatttctg accactttca
    43981 gaaacgaagt gggtgtcgat tgcgtctcgg gtcgttcctt caatatccga aacaattaca
    44041 cgttcttctc ctaaaatcgc attgatcaaa gaggatttac caacatttgg gcgaccaatc
    44101 aaactaaact taatcgtgtc gtcgtcctct tcttcagtct cttcagagaa atacttcact
    44161 gcttcatcta aaatatcacc aatccctagt ccatggcttc ctgaaacagg ataaggatcg
    44221 cctagcccta aggaataaaa ttcatagata tcgtttctca tttctggatt atctacttta
    44281 ttgactgcta aaatcactgg tttattactt ctgtataaaa ttttcgcaac catctcatct
    44341 gcgtctgtca cgccttcgcg tccgctgact acacaaatga ttacatctgc ttcttcaatc
    44401 gcgatttctg cttgatgctt gatctgatcc ataaaaggtt catcgccaag atcaattccg
    44461 cctgtgtcga tcacgctgaa ttctcgacct aaccattcac cttttgcata aatacgatca
    44521 cgagttactc caggagtatc ttcgacaatc gagatacgct caccagcgat gcggttaaaa
    44581 atcgtagact taccgacgtt cgggcggcca acgattgcaa ttgttggatt tgccatatta
    44641 tttcctcctc acaagtttcc aatctcactt agtttaacga atgcattgcc aactttcaag
    44701 catagattgt caattaaacg aagtattttt attccttatt gaaatactaa tcgcttttca
    44761 aaagaaaaac tgagacaaaa gcagctgctt tcatctcagt cacagataca aaatcaatta
    44821 tttttcagaa tcatcggttg ctttttcttc tacttcttct ttcaacgcat cacctaaaat
    44881 gtctcccata gtaaatccag tattttcttc tggtaattca taagactcaa cattttttgg
    44941 ttcttcttct gaagctggtt tttcttctaa agctttgata ctcaatgcaa tacgatgttc
    45001 ttctggatgg atctctaata ctttcacttt cacttcgtca ccttcatgaa gcacttcatg
    45061 tggtgtcgca atatgtttat gtgaaatttg agagatatgg actagccctt ctactccagg
    45121 gaacacttca acaaaggcac cgaagcttgt caaacgttta actgttccgt ctaatactgt
    45181 gccaactgga gcttttgtat cgatttctga ccaaggtcca ggctgtgtgt ctttgattga
    45241 caaggaaaca cgttcttgtt caggattgat agacaacact ttgacattta catcatcatt
    45301 gacagccaat acatcacttg gttttgctac atgactatgt gaaatctctg agacatggac
    45361 tagaccgtca attccgccta ggtcgacaaa tgcgccaaaa tcagtaagac gtgcaacacg
    45421 tccactgaga acatctccct catggatagt tgaaagaatt tcttttttct tttcttctct
    45481 ttcgttttcc actaccgctt tatgcgataa gatcaaacga ttttctgatg gttcgatttc
    45541 gatgatttta aagctcaatg ttttgccttt atagtctgaa aagtctgcaa caaaatgatc
    45601 ttccaccatt gatgcaggga caaaaccacg tacaccgacg tcaacgacta aaccgccttt
    45661 gacaacattt gtaacaggtg cttcgatgac cttgccctct tggaaatctc tttcgatatc
    45721 ttcccaaact tttttagcat ctaaacggcg ttttgaaagt agatagctgc cgttttcttt
    45781 atctttgccg atcgtactga taacgactaa atctaataca tcaccgactt ttactacttc
    45841 gctgatatct tctaccggca aagttgataa ttcttttgcc ggaacgactc cttcaactcc
    45901 agccccttcg attccgacaa tcacttgttt gtcttcaatg actaaaactt cgcccttaac
    45961 aacatcgcca acttttactt cttgtacgct tttcattgcg tcttccattg actggttgtt
    46021 ctcttgattt tgatcaaatt ctgtcataac acttgtcctc ctaaccaaca ttctgccgta
    46081 agtacggtac tgtttttagt ttaattgatt attcaaaaaa aataaagcga tttcgttcgt
    46141 tttccttgaa aaaacaattg tttttcattt tttttcttaa tttaaaaaag aagataacct
    46201 tttttcaaaa caacagcttt tattgctgca acgacttctg gaatcgattt gccagtcgta
    46261 tcgatacgaa cagcatcctc tgcttgcttc agcggagaga cgtcccttgt tgaatcataa
    46321 tggtctcttt cctgtatctc tttcgtcagt gtttcaagat cagtcggaat tcctttttct
    46381 tggttctctt tgaaacgtct ttctgcacgc tcacttacgc ttgctactaa aaagattttc
    46441 acttctgcat tcggtaatac agctgtcccg atgtctcttc cgtccatgac aacgccaccg
    46501 cgttccccta tttttcgttg gacagcaact aattcttctc tgacttttcc atgtgcagag
    46561 acttcagaaa cagcttttgt gacatctggt tgacggattt ctaaagtcac atcttttgat
    46621 gctatgaata catgctgtcc atcttcttgt tgcgaaaaag agataggata ctttttgatc
    46681 agcgaaacta gcccttcttc atctgaaaaa gatacattgt tcgtaatagc taaataagtt
    46741 actgcgcgat acatggcacc ggtatctgta taaatataat tcaattcttt tgctaatatc
    46801 ttggcaacgg tacttttccc agatgaagca ggcccgtcga ttgcaatact gatttgtgtc
    46861 atgatataac tcctttttct cagaaaaaaa gccgcaaagc gcggctatta ttatttaatt
    46921 cttaattctt gaccagggta aagcataaag ttgtttggat ctaagccatt caattcaaac
    46981 aattgatcag tggtgatccc tgcacgctca gccacttgtt ttggaccttc gcctgcttga
    47041 acagtcgttg ttgaaccggc tgcagctgat gaagaaggtg ttgtttctgc tgcattgctg
    47101 cttgaatccc ctgcttgata agttgcctca cttgaggaag tcggtgttgt ctctgctgca
    47161 atactgcttt ctgacgttac acctgtatct tgagacacag tagattcttc cgcgattgta
    47221 ctagattcta ctgttgaagt ctcagttgac tcctcttttg ttgtacttgt agaactttct
    47281 actactgatg tcgaagtttc agaagaagtt gctgctgttg cattgttgct tgatccattt
    47341 ctgatccaga aataagcacc tgctggaata gcgatacata agaccagtaa aatcaaaaat
    47401 agcgaaagaa ataatgtatt tcctttcttt tgttgtctct gagaacttct tgatgaaact
    47461 tcatcatctg tatcgtagat tggttgttcc catggttctt gtgtttcgtt attttctgta
    47521 gattgatgtc tatcttttct gctcactgtt ttacctccta aactctatcg tgtgtattat
    47581 accataggaa aaagtgtctt gtcctaaaaa atcaatgttc catgtttttt ttaaataaat
    47641 ttagtaaaat cgttccccaa tgaagttgtt cagtcttaga aaaaagcttc ctctttggca
    47701 gaagttctgc ttttgctcca tcaatatcac aacaattctg cggcttttcg gagagttttt
    47761 cgccaaaata agctaagatg aattttctgc ggcagttttc ttcattaata tagcgaagca
    47821 tctgctgcag gcggaattct ttttcttttt cctgacgttt gagccgttcc acccaatttt
    47881 ctggtttttc agactgctgg atcagctcca tccatttttt ttgcagctca tcaaagggag
    47941 cttgctctgt ggcatattcc aaataaagct caaaactttg tcgctgttct ctagaaagtt
    48001 gattaaagaa ataatggata cgttcatccc cactttgata aaaaagaatt gctgcacttt
    48061 cttcctgatc tctgcctgct cgaccgattt cttgtacata gttttctaga ctgtctggca
    48121 aatcataatg gaccacataa cggatatcag atttatcgat ccccatacca aatgcattcg
    48181 tagcaatgag aaactgcagc tgatttttta caaactgctg ttgaagttgt cttctttgag
    48241 ctgcatcaag accaccgtga tagtatccaa ctgtaaaacg ttccctgaat aaatgataca
    48301 atcgttcgac ttcttttttt gttgcacaat aaatgattgc cgctccttgt gctctttcca
    48361 tgaagtgttc aagttcttgt tctttttgct gcgttttccg aacaaaaagc gagatatttt
    48421 gccgattgac tgattgtctg atctcttttg gtggtcgatc aaatagtaca tgacgtatat
    48481 ctcttgctac taaatctgtt gctgtcgccg ttagtgccag tgtgaccggg tttcctaaaa
    48541 gctttcggat ttttcctaac tgttgatatt ctggacggaa atcaactccc cattgagaca
    48601 cgcaatgagc ctcatctact acatataacg caatattttg ttttttcaga tgctctaaaa
    48661 gagaaggatt agtcaacatt tcgggactca aaaacaaaaa tttatattgc gatagatgac
    48721 ggaggacgta tcttctttca gattgcgaaa gtaaactatt gaaagcagct actcgtttct
    48781 cccctcgttt ttgtaattgc gtcacttgat cttccattag agaaagcagc ggtgacacga
    48841 tgatgaccag accttccatt aaataccccg tcaattgata gcaaaggctt tttcccgttc
    48901 ctgtaggcaa aatagcgaga gtatcttctc catcaagcaa cgcctgaata atttcttctt
    48961 ggccgggacg aaatgaatca aatccaaaat atttttttaa ttcatttatt aacattctgt
    49021 atcgcttcct ttctcaatag atagatttga tacaaacgaa attctccata atctaattgt
    49081 ccatctacat tccattcgct aaagcgccac tctcgcacgg aatccttatt tggttccaaa
    49141 cgttcgattg tttctgcaga gagaatccac tcgtatggaa aatcatcaaa aaacaaggcc
    49201 cactcaataa gatgatctgt tactgttcct ctttttaaat gacggattgc catgatctgg
    49261 tcaattgtct gaccattcat aaacatctgc ttggtgatca tcatgctttg attgaaattt
    49321 tgctgtaata atgaatcgat caaagcgtgc caaagcgaat ctggccgttt ttcgatttgt
    49381 gctaacaaaa ggtggataca tctgctttgt tgcagataaa ccgctgactc atcattgttt
    49441 tttgccagct gataaggaag cagccctgtt cgctcatttc cagataattg gttagctaaa
    49501 aaatctgctg cttcttctgg tatctccgaa aacagctgca ccaaactctc atacgcagta
    49561 tcaatcaaaa tttcacgtgg caattggctt tcactaagcc atttttttag ttggaaagta
    49621 taaaaaggac ttgtctctgc tggaagatag tcttgaatac ccgttgccag attagaaatt
    49681 acttgaacag caaatttaat gagccgccaa ctagtttccc ctgttctgcc atagcggtca
    49741 aaacgcaaac cagtatgttc tatttgatgt tcggacaaca tatcagcccc tgcaggcgtt
    49801 agctttgctt cattttcatt tatttcgatc caaccttgct gcgcaatttt ttgcaaaatt
    49861 tgataaaact tatcctgctt caattctgga aaactgttat ggataaacaa aagttcgtgg
    49921 gtaaatccat aaataagtac tgaagtcgtt cgttttccca caaggagttg gtaaagagaa
    49981 ctaatccgca gcttgtgtcc tgatgaaaat agcgataaaa taaaaacaat catattattc
    50041 tccttaagtt aggatggtta gaaatgtcac tactatgtaa acttgtacct gaacgctgta
    50101 ttgcatgcgg tctttgtcaa atcgaagcgc cagatatttt cgattatgat gaagaaggaa
    50161 tcgttttatt tgcccaagaa ccagaggctc atcaacagtt cgtgatgccg gaagaagaaa
    50221 catatgtaaa aaaagcgtat caaaaatgtc cagttcgagc gattttatta gaaaataaaa
    50281 tataaatata aacgaaactt taaaaacgta cactaagttc tctaaacttg aaaataagcc
    50341 gggatctgtt tctcttgttc cgtgaaatcc cgacttattt tgaaagattc atttgctatc
    50401 tatagttaat tgactagttt tttcacggac cctataggac ccatttatat tatttcaact
    50461 ttttcagcca gttttccaat gtattgacga tcacagtttg ctggtctgcg ttagaaattt
    50521 ctgcctctcc atctcctttt tgctcaccat agctgccaaa acctccatga tttccgccat
    50581 tgattgatac atactctgtt ctttccggta aatactgctt agcttcttga taagcttcct
    50641 gattcaatac tttatctttt gtcgccgtga tcgataaaac aggaacagct gattctgtca
    50701 atgttccttt tttgtcggga taacttgcca tgaagaatac cccttttaaa tgtttgtctg
    50761 aatgattctt attgacataa cgacttgcca ttacgccacc taatgaatgg ccaccgatca
    50821 cataatcgga atttggaaaa tcttctttga ccttatccgc tttgtttccg cccatcactg
    50881 ctagatcaag tggcattttg acaatagcga ctgaaaagcc gttttttgct aaatcttgcg
    50941 cccaaatact ataactttct ggctctacga gagcaccagg ataaaaaata agtaatggag
    51001 actttccatg attctttttc tctccttcaa aaaataagta atcttctttg ttttttcctt
    51061 cttttaccaa cacttgcgct tctgacgatg gttcgtacgt attttgctgt atatagtaat
    51121 aaccggctcc accgagtaat cctaaaatga tgactaagcc aattagtatt cgtctaaata
    51181 ttttcacttt ctcccacctt ttctatctga gtgacccctt tttcttaatc aagtatgatt
    51241 ataccttaaa aaaataagag agaaatgcaa actttccagc atcaaataaa aaaagacgga
    51301 atatccattt gaatatccgc cctttttgta tgtttatttt tatttagcac ataaacgata
    51361 cacagcttct gcataaattt ccattgcttt atacatacta cttaattccc atcgttcatt
    51421 cgcctggtgc ataaagtcag gagtatcagg gaacatcgca ccgaatgcca cacactggtt
    51481 catcgtacga gcaaaagtag cgccgcctga tacaaacggt tcagtcatgt ctccagtaat
    51541 atcgcgatat gtccccaata gatttttcac taactcactg tctagtggca cgtataatga
    51601 atcaaggaag tcaaattctt cataagtcaa cccatatttt tctgctgttt tagttaattt
    51661 agctactaaa tcatctttct tgaatgtaac agggattctc atatctacac cgatttttgt
    51721 ttgttctggt gtgatttcca aacttgcaaa gttcatcgtt agctcgccgg attgttcatc
    51781 ttctgttttg cccacgacag cttcaccagt tgcattttct tgaactaatt ttcctaagaa
    51841 attgattggg ccaaaatcaa atacttctga aagagcaatt gccagacgag aaatagcatt
    51901 cactccttgt gctgcatctt tcgcgtggat acttttacca agaacgacga tgccttcacc
    51961 ttgttcttcg aaatcgaatt cgtgtttttt caatgcttct tttacttcag aaagcttttc
    52021 tccagagtaa ggagctgcat caggaacaac attcaacgct cctccagctt ttacagaaaa
    52081 ctcgttcgtt cctggtcctg tcaagtaagc ttgcaataag cctttttctg cataaattaa
    52141 aggaaattca gcatctggcg caaatccttg cgtaattcct tcttcttttt cgttatattt
    52201 ttctaaacaa cgccataaat tttcttcatc tgtaccaaag ataaaacgaa tacgtgtctt
    52261 gaattccacg ccagcatcaa tcaaagcttt gacggcatac attgccgcaa tagaaggacc
    52321 tttgtcatct tgggatccgc gaccaactaa ccagccgtct tttatagttg gatcgaatgg
    52381 ttttgtttcc caattatttt cgtcccctgc tggaacaacg tccatatgac acaagatacc
    52441 gaataattca tctccagagc caatctctga ataaccgtaa tagccttctg gatctttaaa
    52501 tgttctaaat cctagctttt cactgatttc caatacttta tccaaagctt caatcacttt
    52561 tttaccgaag ggatgtcctt gtcctgaatc tgcttcatct agtacggaag gaattcggat
    52621 caattcgctc aatgattcta atgcttgttc atgatgttgt tctgtcacaa attttttcat
    52681 gatattatcc tcctaatatg ttaaaaaggg tcatgtcaaa ttcctccgac acaacctctt
    52741 cttctcaaat aatgacgttt taagcctgct ccatcagcaa gcagaatgtt cttaacaaat
    52801 gatgaaagaa aaaacactca ctgaatggag ctattgactt atccacgtgc ttatcgatag
    52861 ccgtttatcg gtttcttcat tcttatgata gaccttgtac ctgtatttgt ctaggtttag
    52921 aacattgctg ctacacctag gatcacacag atggctacaa ataatatcgc aattaattta
    52981 ccggtaaatt tccaccacac tgaaacatcg aaacgagcaa ttgctacagc acccattacg
    53041 atagcagatg ttggtgtaat taagtttact aaaccagatg cagattgata agcagtgatt
    53101 actaaatcct taccgacgcc ggcaaattca cccataggtc ccatgattcc cattgttgca
    53161 gcagctaagc cggaagttga aggaatcaag aatgacatag ggatatagaa aataaatgta
    53221 ataatgatga atacacttga agataatgaa cttaatcctt gttcgcccca atgtaagatg
    53281 gtgtctgtga ttaatccatt gttcataacc acttggatac cacgagcaac agctactacg
    53341 attgctacac cgatcaaatc agaagcacca gctaaaaatt cagagacaaa ttcagactct
    53401 ttcatgccat aagtgattgc tactacgatg gacattacta agaataacat agtaatttct
    53461 gggaagtacc agtcacctaa tggaagcatg cttttaccta gaaccgtacc tagtaccgga
    53521 aggtttgtta accagtttgt caatgattca aaaatcgtga aattcgcatt caaggttgtc
    53581 caaggaatca aactgatgat catgatacca aaagtaataa agaacaatac attaacatgt
    53641 ttttgttttt tcgtgattac ttcttgacga ccgattgctt caatatcgaa atgcttcata
    53701 tcttctttac gttggttgta tactaatgat tttgttggat ctttttcgat ctttgatgca
    53761 taacgataca catatacaat agaaatagct aacaaaataa cgaacatcaa gaatcgaaga
    53821 ccaatacctt cacccatcga aatcccaaca gcttgtgaag ctacgcctgt tgcaaaagga
    53881 tttaccgttg aagctagaca gcctacttga gaccctacta atacgattgc tacagctact
    53941 actgtatcaa agcctacagc catcatgact gggatcagta aagcatagaa tggaatcgtt
    54001 tcttctgcca ttccgtaagt agagccgcct aatgcaaata acagcatcaa tactggaata
    54061 agcatttttt cttttccttt gaatttcttc acaactgaag caatcccggc atctagtgcc
    54121 cctgttttgt tgataatacc taaaaatcca ccaacaacca aaatgaagaa agagattggg
    54181 attgcagcag gtgtttcttt agttagctcc cccgctggta cacctagcat cccattgatt
    54241 ggcgccatga aaatatccca cagcccttga ggattttgtt gtactcgttc atatgatcca
    54301 gcgacgatgt tgcctgcatc gtctacacta tagtgcccag ctggtataaa ccaagtaagt
    54361 acagcgatta ctgcaataat aataaataag accgtatacg ctgaaggcat ttgaaatttt
    54421 ttcttttttg tttgttccat cttcttcttc ctttcttttc cgttagagac aacaattttg
    54481 gctctaatgt tattttatca aaaagagtat cagaacaaaa ataataacac gcattttgta
    54541 aaacttttgt tcaaaaagtt cgtttttccc tattccctcc tcttttttca ggtttttaaa
    54601 gctttcttct ttttcattgt agcgtttcct tttttttata aacaaacatt ttcgtctttt
    54661 aaaaggagaa gaatttcaca tttgcaaaga attgattgat ttttatgcat aaatcaaaaa
    54721 aatactcaaa acaaacaaat aaaacgttta cattattgca tgtcttgtat ttttttccaa
    54781 aaaaaaagac caagaaattt cttgatcttt ttgatattta tttgactgct gtatgacgtt
    54841 gttcgaactg atgttgtttt cgacttaacc atggaagaag ttgcgcatgc agcactaaaa
    54901 agacagcact cacgatgaat cctttgatca agttgaaagg aacgatccca atcagtacat
    54961 agtttgctaa agacatgccc agcatctgtg ttgcgttcag ccctaaaaac attaagtaaa
    55021 gaggcgtaat aacgaaataa tttgcgatac tcataaaaat agttagagcg atggttcctg
    55081 taatgacacc taaaaatttg tttcttcctt tatgctgttt ctttacacta aagaaataat
    55141 aaatcgggaa tgtaaagatc gttgttgata agaaactagc agtgtctcct accaaattat
    55201 ccggagaaaa acctgtagtc agcagatgta atactgaacg caagaacgct gttgcaactc
    55261 cggccgctgg accgaataag aacatgctga tcaataccga gatatcacta aaatcgatct
    55321 tcaaaaaact aaaagccggc atgatcggaa aagcaaaata ctgtaaaact aatgcgatcg
    55381 cagcaaacat cgctactgca accattttct gtacacgggt gttcttcatt tgaaatcctc
    55441 ctgttaggac tcctcttcag cgaacttttt gacgtttctc tccaaatgaa aaaactctat
    55501 tttccaggga aaatagagtt agagttattt gatcaagcgt cgtcaaataa gctctatctt
    55561 ctttatccag actatactgt cggtatcgga atttcaccga ttcagctatt tttttgcaaa
    55621 ataggtcgtg gactataacc accggtcggg aatttcaccc tgcccctgaa gacgaacctt
    55681 atataatttt tgtttacata ttcattatac atatctattc aaaaaacgca acatctaaca
    55741 cttataaaaa gtaagtgttc attttgagtg gttcagcaaa ctgctgactt cttttttgtt
    55801 cagttcacgg taatccccag gacgtaggcc ttgtaaagtc agatctccgt atttttcacg
    55861 ttttagtttt tgcacaggaa gaccaactgc ttgaagcata tttttcactt gatgattacg
    55921 tccttcgtgg atcgtcaatt ccacgatact gtggtttgtc ttaggatcca ctgataaaat
    55981 ttggaattta gctggagatg tttttcgtcc ttcgatcttt attccttttg tcaaaggtgc
    56041 aagcatcgat tttgtagcga tcccttttac ctttgcaacg taaactttat ccacttcatg
    56101 ttttggatgt gtcaggcgct gagcaaaatc tccatcattt gtcagtagca agatccccga
    56161 agtatcgtaa tccagtcgac ctacaggata gatacgttct tttaccatcg gcaaaagatc
    56221 cgtaacaact tttcttcctt tgtcatctgc aacagcagaa ataactcctt taggtttgta
    56281 caataaatag taaccgtatt cttcttgata aattgggact tcatccactt ccacagtgtc
    56341 atgtttccca acttttatcc ctaattccct tacaacttgt ccgtttactt ttacacgtcc
    56401 tgcaaggatg aattcttctg cttttcgacg agaagtgatg ccagcgtggg caattacttt
    56461 ctgcaatctc tccattacaa ttattctcct ttattcattc catcaaagaa tagatctggt
    56521 gacacgtctt cttcaagtga aatttccatt tcttgtgtat caggcagttc ttctaatgat
    56581 ttcaaaccaa aataatctaa gaagtagcca gttgttccat ataatatcgg ccgacctggt
    56641 ccttctactc tacctttttc ttcaatcagt ttatgggcag ctagacgctg taccgctcct
    56701 gaaacctgaa ctcctcgaat ctgctcgatt tcagctcttg tgattggctg tttataagcg
    56761 atgattgcta aggtttcgac ggcggcttga gataatgtgt ttgatgtccc ttgcgcatat
    56821 cgcttaagta agggagccag ctctttttta gtcgttaata cataatggct ttccgtttca
    56881 aaaatatgca aagctgagtg cgagttttga gtgtaagatt cttttaattc tttcactaaa
    56941 cttgtgactt ctgcttcgct gagatttaac agtccgctta attcttcatt actgatccct
    57001 tcctcgccaa caataaaaag aagggcttca agttcactta atttcgtcat gttcggtatc
    57061 tcctactgct ggataaagca taattgattc atagttttct ttttgttgcg caatgatctg
    57121 cccttttttc atcaattcta gcaaagccat aaaagtagtg acaacttcac ttttagaagg
    57181 tgactcaaaa aaagaatcga aagtacagcc atgcgcattt ctttttatac gctcttcgat
    57241 aaataccatt cgttcttcga tcgttgtgtc atcgggcgtg atcgtttttt ccactttttg
    57301 agaaaatttc ttacgttcta agattttctg catggcacgg atcaaatcga tttttttgac
    57361 ttgtcctcgc ttcaacggct tttcagcatc cgctaaccca tcgatgttca taggttcttt
    57421 tgtgtaataa agacttcgtt cctctgcttt ttctgtcagt ctttcagctg catatttata
    57481 tttacggtat tctaaaagct gagtgaccaa ttcttctcga ggatcttctt cccataattc
    57541 ttcatcgtct gtgacgaatt cttggacagg aagcaatgtc ttgcttttga ttgccatcaa
    57601 tgtagcagcc atcactaagt attctcctgc aacagctagc tcgagtgttt tcattgtatg
    57661 gatataattc atatattgct ccgtgatcgt agcgatcggt atatcatata tatcgatttc
    57721 catttgctga atcagatgaa gcagcagatc gagaggaccc tcgaatacat ctaatttaat
    57781 attgatctcc gtcaactcat tcactctttc ttctttttct ttgctagcca ttttccaatg
    57841 taaggctctg tgatttcagt tgcacggatg gagacatccg ggtacttttc ctgtaattca
    57901 tctaacattt gaaagcccat attctcaccg cgataagacg gattcaaaga aatgtcatgg
    57961 atgatcagct gatcgttcgt tgcccagtaa ataccaataa tcccttgaat gttggttgaa
    58021 ccttctggcg taaagaaaaa aagttcataa tcgtcttcag attcgtacgt atcaatctcc
    58081 ttcaacaaag aagttttacc tgtcagtttc ttatggaaac tcaatagtcc catagcaatt
    58141 ttacggtttt ctttccggta atgtgttaac atcttctcac cttcctatga ctaagccctt
    58201 ggaaaatgct gcttataaac atccgtcatt cttttcttcg tgatatgtgt ataaatctga
    58261 gtcgtagaga tatccgcgtg acctaacaat tcctgtactg tccgcaaatc agctccattt
    58321 tctagcagat gtgtggcaaa gctatgccgc agcgtatgtg gtgttacttc tttgtaaatc
    58381 ccagcctctc gtactaactg cttcagattt ttccagattc cttgccgaga aagacctttc
    58441 ccatgattat tgacaaatac atgcatttca gatgcgtcct ttacaagtaa tggacgtgct
    58501 tcgtctaagt agcgttccag ccattggatc gcataatcac ctaaaggaat gatccgctct
    58561 ttatctcctt ttccaactgt ctgtaccagt ccaagagaaa gatgaagatc tcccaatttc
    58621 aatccaatca attcactgac gcgcatccca gtagcataca tgacttccaa gattgcacga
    58681 tctcttatcc ctaacgtttt tgtcgtatca ggtgcttcaa tcagcttttc tacttctgtc
    58741 agagtcaatg tacttggtaa tttctgtgct ttcttcggcg tatcgatatg ttgcatcgga
    58801 tcatgatcag tgatacgctc ttgccgtaaa aactgatgga atcttctgag acttgagatc
    58861 atccgagtaa cagttgctgt cgcttttttt tcttcatgca acttctccag aaaacggatg
    58921 actaaaaagc gatcgacatc ctgccatgaa gcaattttct gctcctgtag aaaatgcaag
    58981 tactgttcca aatcacgttc atagctttgt cgtgtattgg cggacaatcc ccgttcaata
    59041 ttcaagtagt ggagataatc gttgatttgc tctttcataa taatccccct catgcctctc
    59101 tatcatatca gataccgctt cattctgata gtctttttcc ataatctgag caaagaaaaa
    59161 gccgtctaga cactcagacg gactggtttc ttttttcttt ttcatagcat tcctgacaga
    59221 ttccatggaa cgttagccga tgatctttta ctaaaaaatg ataacgttct tctactactt
    59281 gctccacttc aagtaaaaga tcctcttcta cttcctcgat cgtgccgcag ttcaagcata
    59341 gtaaatgatg atggaaatgt ttggcgcctt cttttctcaa atcataacgg gctaatccat
    59401 cattaaaact tactttatcg acaattttca attctgtcaa gatttcaagg gttcgataaa
    59461 cagtagcaag tccaatttcc ggactctttt ttttcactaa aaagaagatt tcttctgcag
    59521 ataaatgctc tttctcattt tccaaaagca caagcagagt agcttctctt tgcggtgtca
    59581 gcttaaaccc cgcttcatgt agctgctgtt ttgttttttt catcgccgaa atagcattca
    59641 tgaccattta ctcaacccca acaatttata ataattacaa ataaaaaaag atataacact
    59701 atgttaatta aaattattat aagctagtta agaaaaacat gcaagggatt ctcaattaga
    59761 ttttttgact agttttgtca caccgttttc ttgtgtaatc aatccttgtt tcaaaagatt
    59821 acctaatgcg cgcttaaatg ctcctttgct gataccaaaa gctttcatga tttcttctgg
    59881 atccgattta tctgtaaagt tgatttgctg atctgcagaa cgttctaaaa tggttaaaat
    59941 catttgtgca tcatctgata tagcttcata tgctcttggc ttcaatgaaa gattcagtac
    60001 accgtctggt cggacaccaa tcactcgacc ttttatctgc tcgcctagac gaggttcatt
    60061 atatcgttca gaaggatgga taaacccaag atagaaatct tccgttaaaa cgtacgtacc
    60121 tgttaatttc aagcggtatg ctgttcctat cacatctttg tttttcattt cttctgttcc
    60181 atatttgctt aaagatttga atattttttc atctgctaaa ctgccccaaa tacggtcttt
    60241 tgcatcaact ctaagggaaa tcatcaagcg atcccctttt ttaggccata gctctgtcat
    60301 tgtcggcaat tcatcaagag atacgacaaa gtctttatct ggaagaccga tatcgacaaa
    60361 cgcacctaaa tcttttcgtg tagcagtaac gattccaaaa gcgtaatgac cgatacggct
    60421 tttaggaatt tgtgtagtga tccgattttc atgtttttga ttttggtaac cgaatccttc
    60481 aactgcttca ccgattttgt gttcaccttc tgatttgctt aagccaaagg tttgtccgtt
    60541 tttttggaca aagtagtttt tttcattttc atcaataata atacctgtaa atatagttgc
    60601 taataaatcg ttcatttttt tctccctcta tagtgaaagg ttttcttaat acatttcttt
    60661 gctatcataa cataatgtta tctacttgaa tagctgagcc ttggtttgga aaggtgaaaa
    60721 aaggagtatg acaacagttg tgtcatactc ccctttttcg aataaacggt gttaaacggc
    60781 aggctcacag cctctttcat ttgcctaaaa ttaattgctc ccatttcctc cgaaccatcc
    60841 agttttacag ttgatggtta cgatgtaaaa tggcaaagca ctttctccaa tgcctttcat
    60901 caatgcagat tttgttgaag tggcatatag attataatag ccttccccat tttcgattct
    60961 ggctttttct aatctgtaac ctccttcaac gatatatcca cgttggatac atgtagcaat
    61021 cactttgctt tggacacctg gtgcccacgt ccatgctgga ttagatgcat cggcaatatc
    61081 acttgctctg gcagatttga ttggctgatt cgctgagttt gaagaggaag gggtcgagga
    61141 tgccgcttct tgctgtgctg cttgtgcgac cgcagcttgt tgagcggctt gagctgcagc
    61201 tgctgcttga gcggcttgtt ttgcttgttc ttcagctgct tttttcgctt cttctgcttt
    61261 tttcttttcc tcttcttctt ttttctcttt atcagtgagc atcttatcta cttgtttcaa
    61321 ttgttcattc aactgagcag atagatctgt atttttcact ttttctactg cttcttttgc
    61381 cgtcttgtat tggtcgcgtg ttgctgaatc cactactttt ccgtctttaa agactatagc
    61441 tgttttttct ttagctgttt gcaattcagc atattgattt ttagcttctg tttgtgcttg
    61501 ttgaatcaat tttccaaaat ctgtgttatc cgtttcgatt gcttggatct ctttatctgc
    61561 tttcaacttg atgtcatctg ctagatgatc atcgacgatg gcaggtgcta tgaaccactc
    61621 attcactttt tggatatctc ggatcttaga ttggatatct tggtaagttg tttctaactc
    61681 tgaatatcct ttttcattct taagactgga taaatcattt tttagttttg ataaatcttg
    61741 atgaatcata tctgcacgaa taaattgatg gtgatcatct gtataaaagg cagcaagttt
    61801 ctcatgaagt tcttccattt gttgctgtct ttttgcttct gctgctactt cggcttctac
    61861 tttttgatga tggtcataat aagcccagct tcctccaccg agtagaagta ctgctgctaa
    61921 aacgagataa ggtacttttt ttgatttttt cttttgcgct ttatctggat ctgttttttc
    61981 taagtttgat tcttgatgtg ttttttcagt atctggcacg attgttattg atttcgcttc
    62041 tgcttcaaac agcttaggct ggttctcttc tttttctatt tttgcttcaa gatcagtacc
    62101 ctctttgctt acagattgat tttcagcatc ggattcacta tttatttcat cgggctctgt
    62161 tgactttttg tctgtgtttt cgagtatttc ttttttctct gcttccggca gtttattcgt
    62221 tttttccttt tctaattgtt tttcagtaaa tacttcagtt ttttctgtga tatccgtttt
    62281 tacgtctttt atttttactg tttctttacc tgtggcgttt tctctatcag agtctttttc
    62341 agtttcttgt tccttttctg gctctacttt gttttcctgt gcaatttttt cactgatcat
    62401 tttgagcagc tctttcgcgt gctcttcttt tgtatcttca cgatgtgctt tgatataagc
    62461 ggatagtatc gaattttctt cctcattatc gacgatttct ttcagttcag ctactcttct
    62521 tttttgtttt gattcaagcg tttcttcttg attctttcct gattcttccg aagtctcatg
    62581 aacagttgct ttcttctgtt catttttctc tatattagtt acttgatcgt gagcaggtgg
    62641 ttcttcgtct tctggttctg ctttttcgaa taattccatt acagattcta gaggtaaatc
    62701 tttcaattcc gaccattcga ttggatcgtt taattctgta tcaggaaaga ctgcatcttc
    62761 agttgattta tcctgcatag ctgcatctgt atttgtttct tcaattgatt cttttatttg
    62821 ctgatcttct gcagttattt gatgaccgca gtctggacag atagtattct gctccatttt
    62881 tttcgatccg caatttggac attttttcat cacttagcga tcggctcctt tactaggttt
    62941 acgctaccat ttaatctatc atattctata cgtcacgcca atagcttatt tttaggattt
    63001 acttaaaaat aatgaatgaa tttccttcct atagaaaaaa aagcttgaga ctgcgtagtc
    63061 tcaagctttt tttcttttaa aataaaccaa acagttttac tgatttctct tctggtcaat
    63121 agtccacgct tattctaaga aatcttttaa ttgttttgaa cgagaaggat ggcgcaattt
    63181 tcttaatgct ttagcctcga tctgacgaat acgttcccga gtaacaccaa atactttccc
    63241 tacttcttcc aatgttctcg tccggccatc atctaaacca aaacgtaagc gaaggacatt
    63301 ctcttcccga tcagtcaatg tatctagtac atcttccaat tgttctttta gtaactcata
    63361 agctgcgtgt tctgctggac ttgttgcttc ctggtcttct atgaaatcac ctaagtgaga
    63421 gtcatcttct tcaccgattg gcgtttcaag ggaaacaggt tcttgcgcga ttttcaagat
    63481 ttctcggact ttttctgttg gaagatccat ttccgcaccg atttcttctg gcgtaggttc
    63541 tcttccaagg tcttgcagca attgacgttg gatacgtatc aatttattaa ttgtttcaac
    63601 catatgaact ggaatacgga tcgtacgtgc ttggtcagcg attgctcgag tgatggcttg
    63661 acgaatccac catgtagcat aggttgaaaa cttaaatcct ttgcggtagt caaatttttc
    63721 aactgccttc atcagtccca tgtttccttc ttggatcaag tcaaggaact gcatgcctcg
    63781 tcctacataa cgtttagcga tacttactac taaacgaagg tttgcttctg ccagacgctg
    63841 tttcgcttct tggtcgcctt cttcgatttt caatgctaac gctacttctt cttccgcagt
    63901 taaaagggag acacggccaa tttctttcag atacatacga accgggtcat tgatttttac
    63961 accggttggc gcagaaagat cttctgtttt tgcttgttca gcttttttct cagcactctt
    64021 caagctgtga attgatggat caccattttc atccaccaca ctgatacctg cgtcttcaat
    64081 cttttggatc aatttgtcca tttcatctgc attcaaagtg aatggtgtag caagctgatt
    64141 ggataaatca tcatatacaa cttgtcgttt gggtttgttt tctttgatga aagctgctac
    64201 tgctgcttca tacgtttttt tatctgttcc atttgccata aaagaaggcc ccccttgagt
    64261 agctatttgg cttgttttaa ctgtttcgtt aaattgatga tttcaatcgt caattcaagc
    64321 tcaagctgtt tgtttccagt tcgtctggct tcttgctgca tttgtttttt taatgttatt
    64381 tcatcagcta agccagattt gctgatcaca tctagcaagt cttggatttc tcgtggcgaa
    64441 ctttcttctg ccaccggtaa atattcgatt tccaccacca accgccttaa attttcatct
    64501 tgcaaaaagt ctaaaaactc tgcaaggttg aattcataat gttgtgagac atatgcatcg
    64561 aataaaaaat aaacttctgc atatgcatca tgaataaact gaaattctcg ttcttttaaa
    64621 atattacgga cagaaacttc tttaaatgca cgatataata gaatctgctc tgccttttct
    64681 acttgcgtca aaggtctctt tttccgtgca gtttcttgtc tcgtaacctg agtgtgttga
    64741 ctttcctgag gttcttgacg attttgctga tttgtccttc gaagatcgcg cagttgtttt
    64801 tgtatcgtct ctctgccgat ctgaaactct gttgacagtt gagatagata catatcttgt
    64861 tcgattggtg aaacaactcg gctaagttca actagtaatt cttcgaggta ttcgatcttg
    64921 tctttttcat tatctaaatt tttcccttgt tttaaataac gtgatttaaa agtaaagact
    64981 gtttcgcgcc catgagccac taattcttgg aaagatgctt ctccatactt acggagatat
    65041 tcatcaggat caagcttttc tggaacgact acgacagaaa gtgctaatcg actgttttgc
    65101 cgaagcaatt caatccctcg attggttgct tcaaaacctg cgttgtctcc gtcatagcaa
    65161 aagaccaatt ctttcgtcag cttttccatt tgttggatct gttgagctgt tagacttgtc
    65221 cccatagaag ctaatccagt tttgatacca gcctgccatg cagcaatcac atccataaac
    65281 ccttcgaaca acaaaatttc tccgtttttc cggatatcgc ttcttgcttt atcaagatta
    65341 aataaaactt gtcttttatt gaacagttct gtttctggac tattcaagta tttgggctga
    65401 tctttttcct gccctttttc aggtgcaagc cagcgtcctg aaaaaccgat cgtctggcct
    65461 tgagaattac gaagtggaaa catcactctt tgataaaaac gatcagacat acggccatca
    65521 tcatgcgtga caaacaatcc agagcgttct aacaattcat ttgaaattga ttcattcttg
    65581 aatacttgct gaagaaaaga acgctcatta ggtgcaaaac ctatgttgaa ttcttcaatt
    65641 aattctaaag acaacccacg actttgcaaa tattctaaag ctggctgtcc agcttttgta
    65701 tgcaatagca tgtgatgata gatttccgct gctttttcat gagcagcaat cagtttcccc
    65761 gtagcagatt caggatttcg gacagctata gtacgctgac gccattgatc ttctaacggt
    65821 atctgttcga agtctgccac ttttaccact gcttctggaa aacttagacc ttctagttct
    65881 tgaagaaaag taaacacgtt tccgccttta ccacaaccaa agcaatgaaa aatctgcttg
    65941 tcttctgcaa cagaaaaaga cggtgtacgt tcttcatgaa aaggacacag ccccaagtaa
    66001 tttttaccag actttttcaa ctggacatat tgtccaatca cctcaacgat attcgtttga
    66061 ctgcgtactg tttcgataac ctcttgagga atccgttgag ccattttttc accccctgct
    66121 ctattgatat cttttgggtt taacagaaga tatttcagga gaataaccgg taaaaaatcg
    66181 cactaactga aatcagtgcg attttaaagg acacaattat acatgtttga ttgtatcata
    66241 tttcattgct ttttcaagta taacacattt ctatctcatt aagcatcaat ttgctactct
    66301 tttcagtaac tgattttgaa tatcagcctt ccagtacaat actcgaagaa caatatttac
    66361 gagcatgaat acaaagaagt atcctacgct ctctcccaac cctaagttta cttccaaaat
    66421 tccatggaac agcattgcag ctacatatat ccctacgatc gtcgttactg ctaggaagat
    66481 gcgcaatggg ttgaagggat aacaagcttt tacaactgcc atgcaactga tcccaatcag
    66541 caagtaatac atcaaagtag tagtttcagc atttccccat gcgaatgttt ttccaagtag
    66601 atacaccgca atgatattca ctactacaag caaggcattt ggtaaagcat taataagtgc
    66661 tgtaggcaaa taacggtaag tgatttttct tttatcttgt tcaaaagata aaaagaagga
    66721 aggataccct tcaatcgcta aatcaatcag cgtgatttgg atagggataa acggaaatcc
    66781 aattgctgta acggcacata taatcgataa aataaatgaa taaatcgttt tgataaaaaa
    66841 gatgcctgag actttcgtca cattgttgac cactcggcgt ccttcaaaca ggacttctgg
    66901 caatgtagta aaatcagagt ccaacaatac cagattcgca atctgacgtg ttgctccatc
    66961 accttctgcc atagcaatgc tgcaatctgc ttctcttaac gccaaaacat cattcacacc
    67021 gtcgcctgtc attgcaaccg ttctgccgct ttctttcaat tcatttacca gcaatttctt
    67081 ttgctgcgga ctcactcgtc caaaaaccgt atagcgatga gcagcttctc gcacttccat
    67141 ttcttcagta atttgagaaa gatcgacata cgcatcataa ttcggtaatc ctgcacgtct
    67201 agcgatattg gagacagtaa aaggattgtc tccagaaatc actttcagat ccacaccttc
    67261 ctcatgaaga taagctagtg tttcttgagc gttttttcgg attggatcat cgatttcaaa
    67321 cagagcaacc ggctcaacaa atgctggttt ttcttcgttc aattgctgat ttggagcgat
    67381 ccccaacata agtacgcgat agccattcgc ttgggctgtt tttagactgt ccggaagccg
    67441 atcttcttcc accaaacgtt caggcgctcc tacgtacacg actcccagtt ctggaaattc
    67501 catcgcgccc catttgcgat cagaagaaaa tgcgactttg tttgtcacat cataccgatt
    67561 agaaagctca aaataatctc tcatagcccg catagtgatg ttgttatcac tgctttctgc
    67621 cagatacgtc cccataattt ctgggatggc tttctcgtac agcggatgta gtgcttctac
    67681 ccgctggata ctcattttcc cttcagtaat ggtccctgtt ttatccaagc ataatgtatc
    67741 gacatgggcc agcgtttcaa ttgaatacat gtcttgaaca aggattcgtt tttttgccag
    67801 tttggttacc cctgtcgtta atgcaatact tattagcaat accaaaccct ttggaagcat
    67861 gcctaacaaa gctgctgcag atgcaataac agaaattttg atagaagcat cgcggatgaa
    67921 cagtgcttcc aaaaatagta tgatccccaa aggaataatc acgaaactag tgaatttgga
    67981 tactttgcga atcgaatcta caagttcaga acgaatagct ttgtgagtct ttgcctcgct
    68041 agtcaagcgg acagcataat tatctgcacc tactcggata acttcagcca tgacagaacc
    68101 gcttgtcaaa aagcttccag aaagcaattc agctccagtt tctttgacaa tcaaatcaga
    68161 ttcgcctgtc aaaagagctt catttgcttc tgcccgtcca gatataacct gtaaatcggc
    68221 tggtatctgt tctcctgctc cgatttttac caaatctcct aataccagct ctttagtaga
    68281 aatcgattgt tctttaccgt cccggatcac tagtacttct gattgggaaa caatcgataa
    68341 tttccgtaca agatttcttg catgtatttc ttgataaatt ccaataacga tattgacgac
    68401 aataattgct aaatacgcca tgttggaata cgcacctaca aataacagac aaatcccaat
    68461 accaaagttc agaaaattga ataacgtcaa tatattatcg ctgaatattt gttttgtact
    68521 ttttgatgca tcttcctggt agtcattttg ctggccagaa gccattttct gaactacctc
    68581 ttgctgtgta agtccattca ttttcttttt ctccattttc cgccaacttt caaaaataat
    68641 cacgtgttta agtctagcgt gttccaagat taaaaacaac ttgtttttat aaaatttata
    68701 gaaaaacaca cgaatgattt tacaaaaaag atgccaacct ttttgtttga atcacccgtg
    68761 tgctgttttt attatcaatc tatctttact tatattgttc ctccaaacgg ctcatccacc
    68821 aacctagtgc tgtaccggct ataaaaagaa caggacaagc tagtacccca gcaactccca
    68881 atcccgcgta atcggtagga atgatcataa tcgttgaagc aaaaactaca cctaaaatga
    68941 aatggaatag cttgctatat gcctttgaga aaatgtaatc catgattttt gacaatccaa
    69001 gaacacaaag aatcccacca attccgatcg gcacaatcac ttcaagtcgt acttctttaa
    69061 atccgtcagc catcgcttta tacaatccca tatacaccaa aaaattagaa gggctcaatc
    69121 ctgggacgat catacccaaa ccaatcaatc cgccggcaat cagccatgtt ccaaaattct
    69181 gatcgacttg cccgtcaaat aattgagcgc cgtataataa aaacaaataa gccagaacaa
    69241 aagtaacaac catgatgatc acatcagaag tatctcttcc ttttttccct gcttctttcc
    69301 ataacgcagg aacagttcca gcgatacatc cgacaaaaaa ccataatatc gttgtttcat
    69361 acgcacctaa taaaaaactg actaaaaaag aaagtagaaa gatgcctgtc actccgccta
    69421 gaccaacagg cagaaaaaac aaaacatttt ctttgaaatt ttttgtaagg tgggctaaaa
    69481 aagaaatcag ccgctcatac atgccaaaga cagctgctaa tgcaccaccg ctgactcctg
    69541 gcaagataaa tcctgaaccg ataaacattc ccttcacaaa ccgtaacatc cagtctttgg
    69601 ttgtgtaccc cacttcttga ttttccattt tttgcctttt ttccactatt tgcgtactcc
    69661 ttctttcatt taattttatt catgattgaa catttcctgc tattttactt aaagactttt
    69721 tcataggtct ctattatttt ttcattcatt ttgacaatat caggataaaa catgttacct
    69781 ccattatgat agagatagcc gccattgtaa agcaaggact ggatccgcca atatcggtat
    69841 tgtttccctt cctcatttcc taaaagtggg ctcagcacat ctttagaata cgcttctgct
    69901 aattctactg tgttttctcc gccatgattt tttacatagg caatataatc tttcccaaaa
    69961 ttatatgctt ggacagctgt ccataaatcg catccttgtt tctttgcttc tactatcatt
    70021 tccgccaaat atttgacacc ttgatcaata ctgtcagctg gattatcaat gatctcagct
    70081 tctccatgca gactttccga gctctgcatg agatctgtcc cttttccttt tgattcggtc
    70141 aatattatcg ccttgatcaa attctcgtag ttttccatat gataattttt tgcagctgtt
    70201 gttatttcta tttgatagct ttccactcgt tttaccgaac gataagttct tcctcataaa
    70261 aacaacaccg caccaataac cgtcagaatg atcatgtaaa aaagaatacg gataatagat
    70321 aatcgttttc gcttcatttc atcacctagt tattttgaca tcttttttta tcttactatt
    70381 tctttatctt cttggctatt tcttccttga tttctgtgta cgccttaaga aaaaatgaat
    70441 tttcctgtat tttttgacat cttacgaaaa tgatcgttgt ttttcagtaa aaacaattgc
    70501 ccaatgactg atataatcgt tatactttac acagtaggtg attatgtgaa aaaagaaaag
    70561 aaatatgcaa aaatgccaga tgggagcgaa atttactatg aaaagagcgg tcaaggcttc
    70621 cctctctttt tactgcatgg caatgacggc agcggccgct ttttttcaga acaggtccct
    70681 gtactcgaac gctactatac cgtgtatcta gtagatagcc gaggacatgg tcgttcgaca
    70741 aacgaagcat ctatgttgaa ttttcagtta atggccgagg atctgaatac gatcatgttg
    70801 ctggaaaaaa tcgatcaggc agattttctt ggatttagtg atggagcaaa tctagcactg
    70861 gtatttgcca gcagcttccc taaaaaagtt catcgattga ttttaaattc tggaaacacg
    70921 cttgttaaag gcgttcgttt ttctgccaga gtcatcagta atattcatta tgcatgggta
    70981 tggttactct ctttatttcg cccaagtctt cgcaaaaatc ttttagttat caaacttttg
    71041 ctgcatgaca tcgggcttac agaaaatgat ttaaaaaaaa tcaactcccc cactttgatc
    71101 atcgtgggaa aaaaagatgt aatcaagcta aaacattctt tgtatatagc taaaactata
    71161 ccaaaagctt cctttgtgct tgtgaaagaa caaggacatg aattagctag aaaagatcct
    71221 gaacgcttca atcgagaagt tcttcaattt ctttctgaaa cataactatt aggaatagaa
    71281 ggtgatttat ttgttaaaaa ataccataca atggctgaaa aaccacctag gactgttcaa
    71341 aacaatcttt ttgatttctg tcattgtcat tattgtcggt gaattgatgt ccatcggtaa
    71401 aactctatct atccagcaat taggagaaac gtttgccact atacctgtat ggaaaagcgg
    71461 actaatgcta ttgattggtc ttatctctgt gttacctatg gttggttatg atattatttt
    71521 gaatcggatg cttgaacaaa agcagaatcc tcgttacctg tttgagacaa gctggctgat
    71581 caatacgatc aacaatattg ccggtttcgg cgggtttgtt agtgtggggc ttcgctctga
    71641 actatatgga caaaagaaag agagcagaaa cgtgatccaa gcattatcga aagttttctt
    71701 gtttttgatg gctggacttt ctatttacag tctgctttct tttttacttg tagtcttcac
    71761 gccagttgct ccttttttga agcaatattg gatatggttg atcggcggcg gtctatattt
    71821 tcctgtcgtc tttttattta ctactttcaa aaagaaagga ttccttggtg gtacctcact
    71881 taaaactcaa ggacagctat tgctagtttc attgttggaa tggaccggtg tcctgtttag
    71941 tttcctttct gttggttatt taatggaaat ccggattgat ctgtggcaaa caattccatt
    72001 attcattgct gcttcagtga taggaatcgt gtctatgatt ccaggagaaa ttgggagttt
    72061 cgatgtaatg atgattatcg ggttgtccgc tattggtgtg ccaagagaaa tagtcgttgt
    72121 gtggatcttg ctctatcgcc tgttctacta catcattccg tttttgattg gaatcgtctt
    72181 tttcttcaaa aatatcggtt ccacttttga tcagcgttat tctggtatcc ctaaacaatt
    72241 agcaacagaa attgcgcata aaatcgtggt tgtcttatta tacttttctg gaatcatgct
    72301 agtactttct gccactatcc cgcaagcttt tacagagttt cgctggcttc atagtttgaa
    72361 cccattgaaa tttcatttta ttatccaatt tcctagtata ctattgggat ttcttttaat
    72421 cattatgggc agagggatcg cagcacgtgt gaaacgagca tatttgccta caatcttctt
    72481 gattgttcta gctttacttt atgttttatt aagtgatttt agtttcacac cggtggtttt
    72541 cttgtctatt ctgcttctga tcattcttgc ttctaaaaac gaattatttc gggaacaatt
    72601 gatctattca tgggagtggc gtacgatcga tggaatcatt attggtgcac tgtccttgct
    72661 ttacatcgtt attggggtat acaatctgcc ggatttccct catcggcgtc accattttat
    72721 ttcctttttc ttatttcctt ctgaaaagat ttggttttcg ggattgttgg ccatcatcgc
    72781 tgttagtttc atgatcgttc tatttgttca tttccttcaa ggagaaaaga aacagattgg
    72841 cgaagccttt aatgaagaaa aggccctgaa gatccttaca acttatggag gaaactccga
    72901 tagtcaattg atcttcttaa aagataagcg gatgtttgcc tatgaaaaag acggtgaacc
    72961 aactgttctt ctccaattcg cttgcttcaa taataaatgt attgtaatgg gtgatccttc
    73021 tggtaagaaa gaagattttc cagaagcaat cgaagctttt atcgaagaaa cagatcgact
    73081 atgctattta ccagttttct acgaaacaag cgaagagatt gttatgattt tgcatgaatt
    73141 cggctacgat ttcattaaga tgggggaaga agcttatgta gacctgaata gttttacaac
    73201 ttcagggaaa aaaatgaagg gcactcgtgc tgtcttgaat cggatcgaac gagaaggatt
    73261 tacatttgat gtcttacaac caccattttc tactgagcaa atgagtactt tcaaaaatat
    73321 ctctgataat tggttgggtt cacgaaagga aaaagggttt tctttaggct ttttctctga
    73381 agattatcta caaagagcgc ctattgcagt tgtcaaaaat acccaagaag aaattgtcgc
    73441 ttttgcaacg atcatgccta cttacacgaa caataaagta ggaacgattg atctgatgcg
    73501 atatgattct aaaaaagcac cttctggcag tatggatttc ttgttcttgc atttgtttgc
    73561 ctatatgaaa gaagaacata tccaatggtt caatttaggg atggcccctc tttcaaatgt
    73621 cggaacttcc agaaagagtt ttttacagga aaggattgca tacttggttt acgagtttgg
    73681 ttctcatttt tattctttcc atggattaag agaatataaa agcaagtacg ctacaaactg
    73741 ggtctcacgt tatactctat attctagaga tagttggatt gcatatgtta tgattgcttt
    73801 gctgattatt gataatgcgc cagttgagca gacacaaggt agaataagtg gtttgaaaaa
    73861 atggatacga agaaagtatt aaaaactgca taccgataga tgaacactct catgatgaat
    73921 aaaagagact gaagtaaaac gaaagttcat gcttctacaa tctcttctta tacaattgaa
    73981 gaacaaagag cggtccaaac agaaatcctg tttggaccgc tcttttattt taaacatttt
    74041 tcattaccaa aatattcgag tcgcgtttct tatgcttcga tctctgtaac gatacctgaa
    74101 cctactgtcc gtccgccttc acgaattgag aatgtcgttc cgttttcaac agcgattgga
    74161 tggatcaagt caacatcaat cgtcacgttg tcaccaggca tgaccatttc tgtatcttct
    74221 ggcaatgtga ttgttcctgt aacatcagtt gtgcggaagt agaactgtgg acgatagttg
    74281 ttgaagaatg gagtatgacg tccaccttct tcttttgtca acacgtacac ttctgctttg
    74341 aattttgtat gtggagtaat ggaacctggt ttagcaagta cttgtccacg ttcgatatcg
    74401 tcacgttgga tcccccgtaa caacacgcct acgttatccc cagcttcccc gtaatctaac
    74461 gttttacgga acatttctac acctgttaca actgcttttt gtgtttcagg tttgatccct
    74521 acgatctcta cctcatcacc gacacgaaca gcaccacgat caatacgacc tgaggcaaca
    74581 gttcctcgac ctgtaattga gaagacgtct tccactggca atagtaatgg tttatctgta
    74641 tcacgttctg gtgttgggat atattcatct actgtatcca tcaattccat aatagcagct
    74701 tcagcatctg gatcgccttg caatgctttt aatgcggaac ctttgatcac aggagtgtca
    74761 tcgcctggga aaccatattc gctcaataac tcgcgaactt ccatttctac caaatcgatc
    74821 aattcttcat catcgactaa atcaactttg ttcaagaaaa caatcaggta ttttacacca
    74881 acttggcgtg acaataaaat gtgttcacgt gtttgaggca tcggaccgtc agttgcagaa
    74941 acaactaaga ttgcaccatc catttgtgct gcccctgtaa tcatgttttt cacatagtcc
    75001 gcgtgtcctg gtgcatcaat atgcgcgtag tggcgttttt ctgtttcata ttctacgtgt
    75061 gcggtattga tcgtaatccc acgttcacgt tcttctggag ctgcatcaat actagcatag
    75121 tcttgaggat ttgctaggcc ttttttgcct aatactgtag tgatcgcagc agttaaagta
    75181 gttttcccat ggtcaacgtg tccaatcgta ccaatgttaa cgtgtggttt acttctgtca
    75241 taatgttctt ttgccataat ttacaaacct cctaaataat atagatcact gaaaaatttc
    75301 agctctcacc tttttattat agtctttttt tgactaaatc aaaaagaata cgtccgactt
    75361 actttttgta agattcgaac taagcttatt atacgtcaat attggtcaaa gtcaactaag
    75421 agcactcgtc tgtcttatac gcgaaaaaag taccttttat aaataaaaaa atgtgttttt
    75481 tgagaaaaac ttcaagatag tccttaactt tcttttgaag caataagaac aaaatgagga
    75541 aaagtttaaa attcagctgt tgccaacaac tttcaataga cgaaatcttc tatttcgtta
    75601 ataataagga aagaggtgaa gtacatgaaa aaattaattg gcgtacttgt cgttttaggc
    75661 atatttctcg gcggaagttt tgcagcttat cattattttt acggcggcga agcttattat
    75721 acaaaaatca cgacgaatgg cgaaatgtca gctacccaag cagataacgg agagaaattc
    75781 acgacttata cctacaagca agcggcttat aataaagatg gtgaaaaaaa acaggtgacg
    75841 cttcgcgaag aaagagaaca tccattaaag atgaatgcgt atcttaaact caaagtcaat
    75901 cctcgtaaag gtgtattaag ctgggaagaa ataaaggctt ctgaagtgcc taaaaatgcg
    75961 gcagaaaaaa tcgataatta gtaaatggtc ttaaaatttt attagataaa aagaggctgg
    76021 gacagaagcg tttaactcca agaaataaga aggagttgct tctgcttccg ccgtttatac
    76081 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc tcacgctctt tttttgttat
    76141 attcgtcttt tccgatcctt ctcttgacac aattcattta actgatcact ggtacttgaa
    76201 cacctaatat tctagtcatt tttataaacg attcaatact tcatccacat ttttgatcat
    76261 cacggatatc gtaccatccg gattattcac aaattcaatc aaatctgggt tgcgatatac
    76321 atctactggt acgatcagtt cgatcccgtt tgatagcttg aatttttgtt ttccaaattt
    76381 tttctcagaa atttcttgta cttcttttac aggaggtgct tctgggacaa atccttgctc
    76441 ttgtacttct tctttaaaag ccatttttgc cgtgatgttt tccttgaaga ctttttccgc
    76501 aatcatctca tgatccaacc gacctttttc ttctatcgta tcatagacag cttctttcaa
    76561 atcagccatg atatcaaatt cttctgtctc aaattttttt ccaagatgct tggctacttt
    76621 ttggatcact tttacatttt cttctaaaga aggtgcagga acactttcga tcactcttcc
    76681 agaaaaataa agctcttttt tgccagaaaa ctcatatttt ttttcaatta attcgtaggt
    76741 ccaatcagtc aaattcacag cgaatgcttc atctgctttt tgtgttttcg cccctagaat
    76801 tgctcgattc aaaatcaagc ggttgtcgat ccctgcttcg tctgcttcaa caaagtgagt
    76861 gtaagcctca cgataattga ctttcaataa agataagtac atcactgtat ctaattcata
    76921 caagacaaca aatacatctg cactaggtgc ttcttcactt tcttggtaga tatcgaacca
    76981 ccgctgaacg attgcttcac ttgcttccac aaaattttct tgagcgattt taagtgattc
    77041 aacgatcatg ctttcttctg ctagttgtcc agtttttgtt tgagcagaag agattttttg
    77101 gatcttcttt tgaagaaaat cacgaatata ttcttttgtc agatccagct ctttttgtga
    77161 aaagactgga gaaccagttt ctctgtcaat aatatgcaga attgcttttt ttaaatagat
    77221 atccattttc tcattccctt ctgtcttagt ttacggaaaa aaaatcaaaa ataccagaat
    77281 caaaatcatt ttcttttaaa caaaaatttc aagagtagaa acaaaatgga ttcttttttt
    77341 cgaaaaatgc gctacactta tagttgaacg aataaaaaag gagctgaaag tatgaattca
    77401 ttaacaagca cttatcgtct atcaaatggt tacgaaatcc cggtagtagg attcggcaca
    77461 tggcaaacac ctgatgggga tgtcgctgtt tcctctgtta aagaagcgct ggctgcaggt
    77521 tatcgccata tcgatactgc ccaaggatac aagaatgaag aaagcgtcgg ccaagcgatt
    77581 aaggaaagtg gaattcctag agaagagata ttcttgacaa caaaattatg gaatgcaaac
    77641 cacagctatg aacttgtgat gtcttctttt gaagaatcat tgaagaagct ccaaacagat
    77701 tatttagatc tgtttttgat ccactggcca aaccctgttg cttttcgtga taattgggaa
    77761 caagcaaatg cagatacatg gcgagcattt gaagaactgt atgaagcagg taaaatcaaa
    77821 gcaatcggtg tgagcaattt cttaccccac catctagata cactagcaaa aacagcaaaa
    77881 attatgccga tggtcaatca agttttcctt gcaccaggcg aattacagac aactgtagtt
    77941 gagtatgcaa aaaaacatga tatgatcctc gaagcatata gtccattagg cactggtaaa
    78001 atcttcgatg tgcctgaaat gaaacaaatc gctgaagcac atgacaagac gattgcacaa
    78061 gttgctttgc gttggtcgct gcaacacgaa ttccttcctc ttcctaagtc agtgacacca
    78121 agcagaatca aagaaaatac tgaattattt gattttgaat taacagaaga agaaatgaaa
    78181 caaatcgatc agttagatgg tgtagtcggc aaagcaaaag acccagatac tacccaattt
    78241 taagtcgaaa ggtgatttta tggatccaat cgaacgcttg aattctctct cggaagaagt
    78301 tacccagact tttcattccg attttgtctt tttgatcgat gcagagaaaa tccaacattt
    78361 tcctgcaaga aactggacac atgatcaaat catcgaagaa ctgaaaaaac ggttcgatca
    78421 ttcgctaatg gtcaaaccct ggcacgaaca tgaagtcatt tattcacctg aactacctgt
    78481 ttttgccttg ataccgaaaa aatagaaaga atggaacgga ttatgtatat atacataatc
    78541 cgttccattt tttatttttc tttcaaaaaa cgggaaaata cgcaaatttt tctgttgtaa
    78601 tttgcgtata cgcaagtttt tttgcattac tccttttcat ttggctgttt ttttcttata
    78661 atcgaaaggg tatgagcgaa ggagagaaca tatgaagaaa aaacagaaac aaaaattatt
    78721 aaatcaattc cgtccttcat tggatggtgt aagaggacaa ttattccgtt ctctagaaga
    78781 agagagcttg cgacgttatg gtttgccgtt acaagtcatg cttgatccca aaaaccgtca
    78841 aatcttaacg atcggattag ctgggaaaac aaatgaagat acacggatca acgtgccaat
    78901 cgatgacaac ttcaatactg tattaaagag aattcgttca ggagaaaacg ggattttcga
    78961 acgattccgt gacaatcttc ttattgaaat cgtgtcttac tggaatgatc aacgattgaa
    79021 aaacgatgaa ccaactgcgc aagctgttgc gacaccagtt acagaagaaa cagtcagtgt
    79081 gccagaagaa aaaacggaaa aaccaaaagc ggacacaact gaaaatacac gctcaactga
    79141 tacgaatgcc gctttaagtt ttgctgattt ttcaaaagcc gttgctgagt atcctaaatt
    79201 ttacacagaa caaaatgaac atgaagtagt tattaatgaa aaaaatgctg atgaacctcg
    79261 taaattagca actatttcta tgactactga aaatgagttc gttatcgaaa aagcactcga
    79321 acggaaatac aaagtcaaat tgactttgat tcctttgatc gaacaatttg cagcgacagc
    79381 gattactgat cgataaaaat tctatttgtc ataaaaggct gggatttaaa atcccagcct
    79441 ttttagtcat ataataattt ctacagcaag taaaaagaaa ttccttctaa catcaggctc
    79501 aaatatcagc ttgacctgtc caacaaatca cctatgatcc tacaatgtta ggcgacttct
    79561 cttctacctg aagatatctt ggagttgcct gatttcttaa gatgcctaac cttaactata
    79621 aaaaattcta ataataaaca tattttctga tagagaaaaa ag
//
LOCUS       DASNNU010000003        69771 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.003, whole genome shotgun sequence.
ACCESSION   DASNNU010000003 DASNNU010000000
VERSION     DASNNU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.003"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(606..962)
                     /locus_tag="U8116_000170"
     CDS             complement(606..962)
                     /locus_tag="U8116_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEN8433194.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /gene="spx"
                     /locus_tag="U8116_000171"
     CDS             complement(976..1386)
                     /gene="spx"
                     /locus_tag="U8116_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN8433195.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1732..3918)
                     /locus_tag="U8116_000172"
     CDS             complement(1732..3918)
                     /locus_tag="U8116_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN8433196.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4136..4819
                     /locus_tag="U8116_000173"
     CDS             4136..4819
                     /locus_tag="U8116_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEN8433197.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4900..5619)
                     /locus_tag="U8116_000174"
     CDS             complement(4900..5619)
                     /locus_tag="U8116_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN8433198.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5931..6137)
                     /locus_tag="U8116_000175"
     CDS             complement(5931..6137)
                     /locus_tag="U8116_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433199.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6382..6984
                     /locus_tag="U8116_000176"
     CDS             6382..6984
                     /locus_tag="U8116_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN8433200.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7125..8405
                     /locus_tag="U8116_000177"
     CDS             7125..8405
                     /locus_tag="U8116_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN8433201.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8418..9146
                     /locus_tag="U8116_000178"
     CDS             8418..9146
                     /locus_tag="U8116_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN8433202.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9215..10738)
                     /locus_tag="U8116_000179"
     CDS             complement(9215..10738)
                     /locus_tag="U8116_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN8433203.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10990..12171
                     /locus_tag="U8116_000180"
     CDS             10990..12171
                     /locus_tag="U8116_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN8433204.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12231..13859)
                     /gene="treC"
                     /locus_tag="U8116_000181"
     CDS             complement(12231..13859)
                     /gene="treC"
                     /locus_tag="U8116_000181"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEN8433205.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14310..17021)
                     /locus_tag="U8116_000182"
     CDS             complement(14310..17021)
                     /locus_tag="U8116_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433206.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(17031..17732)
                     /locus_tag="U8116_000183"
     CDS             complement(17031..17732)
                     /locus_tag="U8116_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8433207.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17918..18517
                     /locus_tag="U8116_000184"
     CDS             17918..18517
                     /locus_tag="U8116_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN8433208.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18598..19611
                     /locus_tag="U8116_000185"
     CDS             18598..19611
                     /locus_tag="U8116_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN8433209.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19719..20636
                     /gene="rbsK"
                     /locus_tag="U8116_000186"
     CDS             19719..20636
                     /gene="rbsK"
                     /locus_tag="U8116_000186"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEN8433210.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20664..21059
                     /gene="rbsD"
                     /locus_tag="U8116_000187"
     CDS             20664..21059
                     /gene="rbsD"
                     /locus_tag="U8116_000187"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEN8433211.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            21059..22432
                     /locus_tag="U8116_000188"
     CDS             21059..22432
                     /locus_tag="U8116_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN8433212.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22789..24933
                     /locus_tag="U8116_000189"
     CDS             22789..24933
                     /locus_tag="U8116_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN8433213.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24985..26025)
                     /locus_tag="U8116_000190"
     CDS             complement(24985..26025)
                     /locus_tag="U8116_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN8433214.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26302..28104)
                     /locus_tag="U8116_000191"
     CDS             complement(26302..28104)
                     /locus_tag="U8116_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433215.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28118..28870)
                     /locus_tag="U8116_000192"
     CDS             complement(28118..28870)
                     /locus_tag="U8116_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8433216.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28879..29241)
                     /locus_tag="U8116_000193"
     CDS             complement(28879..29241)
                     /locus_tag="U8116_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN8433217.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29417..30289)
                     /locus_tag="U8116_000194"
     CDS             complement(29417..30289)
                     /locus_tag="U8116_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN8433218.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30340..30828)
                     /locus_tag="U8116_000195"
     CDS             complement(30340..30828)
                     /locus_tag="U8116_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEN8433219.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(30966..31757)
                     /locus_tag="U8116_000196"
     CDS             complement(30966..31757)
                     /locus_tag="U8116_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEN8433220.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(31812..32420)
                     /locus_tag="U8116_000197"
     CDS             complement(31812..32420)
                     /locus_tag="U8116_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEN8433221.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32413..32730)
                     /locus_tag="U8116_000198"
     CDS             complement(32413..32730)
                     /locus_tag="U8116_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN8433222.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32886..33614)
                     /locus_tag="U8116_000199"
     CDS             complement(32886..33614)
                     /locus_tag="U8116_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEN8433223.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33773..34654
                     /locus_tag="U8116_000200"
     CDS             33773..34654
                     /locus_tag="U8116_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN8433224.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34891..35934)
                     /locus_tag="U8116_000201"
     CDS             complement(34891..35934)
                     /locus_tag="U8116_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN8433225.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35937..36605)
                     /locus_tag="U8116_000202"
     CDS             complement(35937..36605)
                     /locus_tag="U8116_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN8433226.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36747..39083
                     /locus_tag="U8116_000203"
     CDS             36747..39083
                     /locus_tag="U8116_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN8433227.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39198..39509)
                     /locus_tag="U8116_000204"
     CDS             complement(39198..39509)
                     /locus_tag="U8116_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433228.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39690..41165
                     /locus_tag="U8116_000205"
     CDS             39690..41165
                     /locus_tag="U8116_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEN8433229.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41212..41796
                     /locus_tag="U8116_000206"
     CDS             41212..41796
                     /locus_tag="U8116_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEN8433230.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41798..42217
                     /locus_tag="U8116_000207"
     CDS             41798..42217
                     /locus_tag="U8116_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEN8433231.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42498..42782)
                     /locus_tag="U8116_000208"
     CDS             complement(42498..42782)
                     /locus_tag="U8116_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEN8433232.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(43134..43844)
                     /locus_tag="U8116_000209"
     CDS             complement(43134..43844)
                     /locus_tag="U8116_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEN8433233.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43841..45313)
                     /locus_tag="U8116_000210"
     CDS             complement(43841..45313)
                     /locus_tag="U8116_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN8433234.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45451..45924
                     /locus_tag="U8116_000211"
     CDS             45451..45924
                     /locus_tag="U8116_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN8433235.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(46077..46628)
                     /locus_tag="U8116_000212"
     CDS             complement(46077..46628)
                     /locus_tag="U8116_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN8433236.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46733..47263
                     /locus_tag="U8116_000213"
     CDS             46733..47263
                     /locus_tag="U8116_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEN8433237.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47746..48189)
                     /locus_tag="U8116_000214"
     CDS             complement(47746..48189)
                     /locus_tag="U8116_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8433238.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(48205..49365)
                     /locus_tag="U8116_000215"
     CDS             complement(48205..49365)
                     /locus_tag="U8116_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN8433239.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49872..51572
                     /locus_tag="U8116_000216"
     CDS             49872..51572
                     /locus_tag="U8116_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433240.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51674..52507)
                     /locus_tag="U8116_000217"
     CDS             complement(51674..52507)
                     /locus_tag="U8116_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEN8433241.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52811..54976)
                     /locus_tag="U8116_000218"
     CDS             complement(52811..54976)
                     /locus_tag="U8116_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN8433242.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            55236..57530
                     /locus_tag="U8116_000219"
     CDS             55236..57530
                     /locus_tag="U8116_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN8433243.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57523..58188
                     /gene="pgmB"
                     /locus_tag="U8116_000220"
     CDS             57523..58188
                     /gene="pgmB"
                     /locus_tag="U8116_000220"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN8433244.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            58181..59188
                     /locus_tag="U8116_000221"
     CDS             58181..59188
                     /locus_tag="U8116_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN8433245.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            59210..60229
                     /locus_tag="U8116_000222"
     CDS             59210..60229
                     /locus_tag="U8116_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN8433246.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60510..61877)
                     /gene="nhaC"
                     /locus_tag="U8116_000223"
     CDS             complement(60510..61877)
                     /gene="nhaC"
                     /locus_tag="U8116_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN8433247.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61986..63407)
                     /locus_tag="U8116_000224"
     CDS             complement(61986..63407)
                     /locus_tag="U8116_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN8433248.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63650..65527)
                     /gene="tdc"
                     /locus_tag="U8116_000225"
     CDS             complement(63650..65527)
                     /gene="tdc"
                     /locus_tag="U8116_000225"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN8433249.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65818..67074)
                     /locus_tag="U8116_000226"
     CDS             complement(65818..67074)
                     /locus_tag="U8116_000226"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEN8433250.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(67186..67391)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            67654..68955
                     /locus_tag="U8116_000227"
     CDS             67654..68955
                     /locus_tag="U8116_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN8433251.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(69024..69623)
                     /locus_tag="U8116_000228"
     CDS             complement(69024..69623)
                     /locus_tag="U8116_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN8433252.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        20946 a        14104 c        12356 g        22365 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1621 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1681 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1741 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1801 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1861 tagtcttcgt tcttcttcta tcgtcgttac tggttctgct tttatttcat taaagtcgat
     1921 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1981 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     2041 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     2101 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     2161 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     2221 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     2281 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggctat
     2341 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2401 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2461 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2521 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2581 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2641 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2701 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2761 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2821 ataattagtt aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2881 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2941 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     3001 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     3061 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     3121 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     3181 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     3241 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3301 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3361 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3421 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3481 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3541 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3601 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3661 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3721 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3781 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3841 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3901 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3961 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     4021 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     4081 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     4141 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     4201 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     4261 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4321 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4381 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4441 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4501 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4561 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4621 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4681 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4741 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4801 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4861 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttagttg taaagattcc
     4921 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4981 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     5041 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     5101 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     5161 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     5221 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     5281 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5341 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5401 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5461 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5521 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5581 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5641 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5701 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5761 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5821 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5881 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5941 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     6001 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     6061 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     6121 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     6181 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     6241 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6301 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6361 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6421 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgtcttagaa
     6481 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6541 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6601 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6661 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6721 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6781 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6841 tctctcatta cgttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6901 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6961 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     7021 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     7081 caattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     7141 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     7201 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     7261 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7321 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7381 ttttaggaga tcacttaaca agcatcggcg atttttttgt tttgaaatct tcggttattg
     7441 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7501 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7561 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7621 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7681 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7741 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7801 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7861 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7921 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7981 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     8041 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     8101 gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     8161 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     8221 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     8281 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8341 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8401 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8461 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8521 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8581 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8641 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8701 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8761 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8821 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8881 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8941 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     9001 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     9061 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     9121 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     9181 agccgatcca aacccttcgg ttcttttttg cctatcagtg caaaagcatt tcctgtgcaa
     9241 tttcacgtcc atccattgta tctggataaa atgttggcca attctccatt tctttcagca
     9301 atacttcttg actttcattt cctgcatata aatgaaaatg ttcggagtca gccggtgcaa
     9361 tcgtgtgatc actgaattga atatatctaa aagcgccact ttccttatct tctgcttcga
     9421 atagatacct tacgccacgg tttcctgcct cgtaggtcaa gatttctttc cctacataac
     9481 gataatttga acgtttcgtt gttccattct taaacgtaaa tgccatctct ttctcactga
     9541 tcttgattga agcaatatct gtttgatagc ccttcgtata atattcttta tattcttgtg
     9601 ctgtcttatc ctgattcaat ttttctttat attcaaaaac aggatcaaaa gtcccatctt
     9661 ctacatacgt atctactgtt tgccaagttc ctgcccaatc tgaaagagag cgatcttgta
     9721 catcctcgtc ttcaaaataa ccgttgtaga cgctcttttc ttttttgggg gtcaaagtat
     9781 cttctagcgg gttttctgtc tctgtcgttt ttttcaacgc ttcgaggttt gcttccatga
     9841 tagaaagata gttttctcca ttttctattt gttcattcgt caatccttcc aaaggattca
     9901 atacttctaa agatacacct gcttcgtttg ctaacgttct ggcaatacta tctttggcgt
     9961 tttcttcaaa ataaatatag ttgattccat gttctttaac aaagtgtttc agttcagcaa
    10021 ttctcgccgc agacggttct tcactagaag aaagaccagc aatcgatact tgattcaatc
    10081 catattctaa cgctagatag ttgaatgcag catgttgcgt gacaaaagat ctttgttttg
    10141 cttgcttaaa ggtttcttga taaagctgat ggagtgcttg aagtttctcg gtataggctg
    10201 ctgcattttt tgtccatact tcctgttttt ctgggtaagc ttcaatcagc tgatcacgta
    10261 tgtttgctac ctgcttgatt gctaaacttg gagctagcca tacatgtggg tctagctcat
    10321 gtgtatggcc ttcttccccg tgttcatggt catgatcatg tccttcttca cttcccggaa
    10381 gtaaaagcat accttctgtc gcttttatcg tatggacatt gccttcttgt aatgtttttt
    10441 ggattgcggg gacccatgtt tccatgtttt catcattata aacaaagata tctgtatctt
    10501 ggatctttgc catgtcttta gcagaaggtt cataatcgtg ggaatcagtc cctgctggaa
    10561 tcaataactc tacttctcct tcatccccga cgattgcttt tgtaaaatca tacatgggat
    10621 aaaaggtcgt catcacgctc aatttttctt tactgtcctc ggctttccca cttgccccgc
    10681 aagcagttaa catacctgct aaaaccagca tggccaatat acctatcaat cgtttcattt
    10741 atgacactcc tcttctcatc ttctttcatt tgaatgttcc gattcgcttt tcgcaaaccg
    10801 taatagttac gttttagcaa caggatttaa tttatcatat ggttaaaaga actgtcaagc
    10861 gttttcttat tttaattgat gagaaagttt atatgaatga ttgacgatat ttttgcaatc
    10921 aacctctatc attttccttt tatacccgct tttgtctata atgaatttat agataaagga
    10981 ggaaaaagag tgaaaaaaga aacagataca acgaatcgtt tgatccgctt tttaggagga
    11041 aaaacctctt actatgtttt aggaatagtt atcttacttg cgctgatcat ttattttttc
    11101 catcagatct cgtttatatt caagcctgtc ggaatcatta tcgctactat cttgccccca
    11161 ttattgtttg cactgatctt gtattatctt ttggaaccag tcatcaaacg tttgagcaag
    11221 cacctttcga ggaacttgtc agtcattatc gtttatgtga ttattttatt tttattgggg
    11281 cttggaagca tatggcttat tccttttctt gaagaacagg caaagacatt gatcagtagt
    11341 ttgccagatt tgtttcaaga ttttcaagag agtctccgac aatttttgga gaaaacacct
    11401 tttgccgatt catttaatca atttttcgct tctattgacg acgtgacgtc tgatatagct
    11461 ggatttattg gaaattattg ggaaagtggc gcacaacgga tcagcaacat tttttcaact
    11521 gttacagcta tttttattac tttatttact ggtccaattg tcgctttttt ccttttgaga
    11581 gatcctcaaa aattttatca gtcagtttta gcgattgtcc caccagcatt tcgtacagat
    11641 tttaaaaatt tgacaaagat tgctaatcaa caattgggtt catttctaaa ggggcaaatc
    11701 attgcttcat ttatccttgg agcagtctac tgggtttgtt tcttactgat cggattagaa
    11761 tttgctagtg ttttagctat ttctgctggg ttgttatgta tcattccata tattgggccg
    11821 tttatcgcct tctttccagg tttgatcatt gcattccaag actctacttt tatggctgtc
    11881 aaatttgtca ttgtctggtt tgccgtacaa cttctccacg gtgacttggt gattcctcga
    11941 gtaatgggtg atcgtttaca gatccatccg attacgatct taatcgtttt acttgtcatg
    12001 ggtgatctaa tgggaattgt gggcgttatt tttggtattc ctatctatac gttagtcaaa
    12061 ttactcgtta cttttatttt ccgtaaattc aaacaacgct acaataaatt ttatggcgat
    12121 aaaggagagt atgaaaactc ttcattttct gaagaagatt attttaaata aaacacaaaa
    12181 cgtcgatctt acgagaatag ctcgtaggaa cgacgttttg tgtttttgtt ttaatttttc
    12241 agcaaagcaa ttgcctggta tggttttagc tccagacttt gatgaagaac agtatcggaa
    12301 taattatcga taagaatatt ggcttctgtg aatccttctg ggatggcgat ccttgtatct
    12361 ttatcataga aatttgttaa tacaagcagc tgctgtcctt caaattggcg aagaaagcca
    12421 taaacttccg gatgatttgc ttcataagca tgataatctc cttgtgcaac gattggatat
    12481 gtttttctca gccgaatcaa tttcttgtaa taggacaaaa tagaatcttc agaagatagt
    12541 tctttttcta cattaatctc ttctagcttg cctccaactt ttaaccaagg tttccctgta
    12601 gtaaatcctg cttgttctgt gtctgaccat tgcatgggtg ttcgagagtt atcgcgagat
    12661 tttgctttga tccgacgaaa cgcttctttt tctgtgaatc cttgttcaag gagtaactta
    12721 taagcattca aactttctac gtcaatatag tcggaaatcg atgaatagtc tgggtcgatc
    12781 atgccgattt cttctcccat ataaatatag ggcgtcccac ggttcaaatg gatcatggtt
    12841 gctagcatct ctgctccttg ttttcgatac tgatcgatag ctacaaatcg attgagcgca
    12901 cgtggctgat catgattatt aaaaaataga gcattccagc cgttaccctc actcatcttt
    12961 tctccccatg tatgaaatag tcgctttaat tctgcaaaat caaatggagg agtagaccat
    13021 ttattcccat tttcataatc gactttcaaa tgatggaaat taaagaccat tgataattct
    13081 tctctactag gttctgtata caagatacaa ttttcgatcg tcgtagagct catttcaccg
    13141 actgtgatga tagtggaatc cgtcccaaac gttgcttggt tcaattcttt tagatacgta
    13201 tgggtgattg gtttgtccgt atattcaggt ttgccatcat tttctttatt atttttcaat
    13261 tgttcgtctt taccgataac attgataaca tcgaaacgga atccttttac ccctttttcc
    13321 atccaaaaac gaacaacttt gaatagttcc tctcttactg cttggttccg ccagttcaag
    13381 tcagcttgtg tccgatcata taagtgcaaa taatatttcc ccgtatctcc aaatggcgac
    13441 caagcacttc caccaaattt tgatacccaa tccgttggct cttctcttaa aatgaagaag
    13501 tcctgataat gctggtcccc tgctagtgct tttttgaacc attcatgttc tgtcgaaacg
    13561 tgattcagca ccatatcgag catgatttct atccggtatt tctttgcttc ggctattagc
    13621 tgttcgaatt cctccatcgt accaaagata ggatcgatgg ctgtgtaatc agagatatca
    13681 taaccgttgt ctttttgagg gcttggataa atagggttta gccaaatcat gtcgatgcct
    13741 agatcattca gatacgggag tttttctttg attcctttta aatctccaat tccatcacca
    13801 ttactgtctt tgtaagattt gggatagatt tggtaaatga ctttgtcttt aaatgtcatg
    13861 gatatttacc tccattcaac tgatttatta cctaccacaa cttatatata caagttatca
    13921 tttattatta taactgaacc gaaagcgttt gtaaaattta tctttataca ttctaagagg
    13981 tctgttgccc aataaaaaac aagagatcag acatgtattt gtctatcctc ttgtttaaaa
    14041 tagcaaaata ttcttgtttc ttctataaat ggtgttcaaa agcttttatc tacggcaatc
    14101 agtcttctca aatgtaaaac atgaggagct aattttattt gagtattctt tttgctcgat
    14161 gcaggacggc ttttttacaa cctctgattc atggtgttca atcagctgtc cttcggtatt
    14221 agctcaaaaa acggcaaaat atgaaaaact attttgccgt ttgtttctct aatactcagg
    14281 tcaaaccgct gatttcacaa cctctgtttt tattcattcg atttcaacgc atcaatcata
    14341 tcgatatgct ttaatttgaa atgggtaaca atcatcacaa taaccgtaaa tacgatagtt
    14401 aatcctgctg ataagacata agcccaccat gtgatcacta atggaaagat gacattctcc
    14461 atagacgctt gttgcaagat gaagtcagtc aatatatatc ctacaccaaa tcctccgatg
    14521 attcctaaaa gagtaaagat gatattttct cgaacaatgt acatcgtgac ttctttatca
    14581 aaaaatccaa ggacttttat tgtagataat tcgcgtacac gctcggaaat attgatatta
    14641 gttagattat acaagacgat aaaagccagc agtcctgata atacgacaaa gattaagaca
    14701 accgagtcta agttcgccat tgattcttct tgcgtttcga tttgtttgga catgaaagac
    14761 gtatttgtca cagcgtctgt ggcaagcagt ttctcggaaa gcatttcttc ttcttttgtt
    14821 gtcatcttat ctgtacgcag caaaaatgca ttatccatca tcgattgatc agtaactgaa
    14881 gcataatatt caggggacat ataaataaag ctacctagat agtttttcac gatttctttt
    14941 acttcgactt tccattcttt ttgatcttca tcataaagcg aaatatgatc tcctgtagat
    15001 aagtcaaata gttctgctac tttttgcgta atagcgattc ctgttttagg caaggagcgt
    15061 gggttctctt caagatcagt taaatggata aaacgatcca aatcagctgt tttacttggt
    15121 acgatcactg tcagactttg cgaagcatgt cctttttttc tcaattctac ggtttgagta
    15181 tgaatagcta ggatctcatt tactttttct tgcttctgca gcatatccgc tgctttttga
    15241 cgactatctt ccgtggatga atccaatgta acgattgctt gatagtcaat gatcggtcca
    15301 aattgtttgg ctgaaacaga acttagagaa tctttcaatc ccacgcctgc aaccatcagc
    15361 ccggcacatc cagcaattcc gataatcgcc ataagcattc ttgacttata tcggaagaga
    15421 tttcggtaac tgattttttg attaaagcta aggcgtgacc acagcggagt gatgtattca
    15481 agaaaaatgc gtttccctgg cttcggtgct ttcggttgaa gcaatgcagc aggccgttct
    15541 cttaaatctt tgaataaaac gatcattgca gcgccaaatg cggcaatgaa gaatgcggca
    15601 gctgcttgta aaatcggcgg ccagtcataa aaaataagcg cactcccaat atcataccgc
    15661 tcgttagaca gctcaaaaat gatccttggc aaaagattcg ttcctaatat cgttcctaaa
    15721 acaataccta tccctgaggc tagacttgca tagattgcat atttttttgc gatttcaact
    15781 ttcgtataac ctaacgcctt taatgtcccg atttccctgc ggttttcttc taccatacgt
    15841 gtcattgtcg taaaggtgat caatgcggca ataaagaaaa agaaaacagg aaaaacattg
    15901 gcaatcgcag cgatacgatc agacagatca ccaaattctt gaaaaccgac attttctgaa
    15961 cgttcattgt ataggtatga tggtttagtt aaattttcaa ttttttctgc atttcgattc
    16021 aattcttcct ctgcagaggc cagttcacgt tctttctcaa ctaattgcct ttcttgttca
    16081 gcaagttgag cttcttgttg agataatgtt tgaacagcga ttgttcgttg cgcttcagga
    16141 agctgctgga tagttgctga ctgttgcgct aactgttttt tagcatcttc taaccgtgtt
    16201 cttgcttgcg ttgtttgtgc tttgccatcc gctatttttt gctgtgccgg ttctaattct
    16261 tcgttggccg attttttcag ctcttctaag cgttcttcgg gtcgatcttg taaatacttt
    16321 tctgcctttt cttgattttg ctccatttta tctttgtaag ctttgctgta cgtattcaaa
    16381 ttctttacgt ttgtaaaatc aagatataag acagaataaa tatctgtttt aaaaacggat
    16441 tcaggtaaat agacaaaata atcgacgcta ccatttccga cgttcgcgta tcctctctcc
    16501 aaattgttga tgaacaatgg agaacggaca aagcccgtga tggtatactc tttttctttc
    16561 aattcatctg aatcaatctg ataaatatca ccgattttat acccttcatc ttccgctttt
    16621 tcatccaata caagctggtt tggtttttcc gggagttttc catcagttaa ttgtagcttg
    16681 ttctgcttgt tatttttgtt ataagaaaat atttgaatca cttcgttttt gccagtattg
    16741 gaataataga tctgttttcc tgtttctact tttgcatctg ggatcttttc tgcttgagca
    16801 atatcttttt gtgtcaagcc accagtagaa acgatttgca aatcagaaag tcctgctgta
    16861 ccaacatagt gatccatcgt tttttgcatg acaggtccag cagttttgac acctacaaat
    16921 aaaagtgttc ctagcatgac aataaggatg attgcaatga atcgtccttt agattgccga
    16981 atttcgcgaa aacctgcttt aagatatgtt cttgttttca taaaatcccc ctaccataca
    17041 atctcatcaa tggatgaagg atgatcattt aattcaacag accttacttt agcatcatta
    17101 atgtgaatga ctctatcagc gatgggcgct aaagcagaat tatgtgtaat gatcaaaacg
    17161 gtattgccgt gttgtctgct agcgttttgc aataatttca atacttgctt gcccgtttca
    17221 aaatccagcg cgcctgtcgg ttcatcacat aaaagaagtt tgggattttt agctagtgca
    17281 cgagcaattg aaaccctctg ctgttcgcct cctgataatt gggacggaaa gttattcaat
    17341 cgatgagcta ggcccacttg cctcaatacc tctacaggat caagcgcatc aggagaaact
    17401 tccgttgcca attccacatt ttcttttgct gttagattag gaacgagatt gtagaattgg
    17461 aaaacgaacc ctacatctgt tcgtcggtaa gcagtaagct gtttatctga aaattgcgca
    17521 atatctgtgt catcaataat gatctgacct tcatcaggtg tatccatccc acctaaaata
    17581 ttaagaatcg ttgactttcc tgctccactt ggaccaagta taatcactaa ttcgcctttt
    17641 tcaattgaaa aagaaatatc gtcatttgct gtaatggtcg tttctcccat tttgtatcgt
    17701 ttgtattcat tttttacttc gacatacttc actctgattc ctccattttc acgatatttc
    17761 tcgctatatc tatgcatcga atgcgttttc tcacacttat ttattttacc actgaatttc
    17821 tctagactaa agaaaggaat cctttaattt agttgacaaa taaacgattt atgatttact
    17881 tacataaaaa acattgttat ttgaaagaag gaacatcatg aatgatttaa aggaaaatat
    17941 gatttttgat ttatcaaaaa ctgcttttgt cgcgatcgac atgcaaaaag agatcgtaaa
    18001 taacggtaca ctctctccac acactgcagc atctattctg acaaacaatg atttgttagt
    18061 aagaacattg aaacgtaccg ctgccttgaa agttttagtg aacgtagaca tctccacttt
    18121 cccttatttg agtcaacaaa ctgatatggg tggaatgact gcttctgtac ctccagaatt
    18181 cactgattta cttttgaagg attctttgaa agatacagat aatatgttga ctatcacaaa
    18241 atacaatcca agtgcttttt ttggtacttc tcttgatctt caacttcgcc ggcgtggaat
    18301 cgaaactatc attttaagcg gtgtagcgac aacaaacggt gtatatgcta ctgcattaga
    18361 tgcctttcaa cacgggtacc atatcgtgct tgctgaagat gcttgcagtg atcgtgacaa
    18421 agaatcccat caattgttca taaaaaagat atttccgaaa actgcacgag tacgctcaac
    18481 gaaacaaata atcgaagcta tccaacaaag caaataagta ctggcacacg tgattttcat
    18541 ttacttcttt ttgatttcat gggaaaataa ccacaggatt tagataggag tatgaacatg
    18601 gtgaaaaaaa agatcacaat caaagaagtc gctaaacttt ccggcgtttc aatcacaact
    18661 gtctccttga tattaaatgg aaatacggag aaattcagtc cgaaaactgt tcagaaagtt
    18721 ttagctgcca aagaagaatt aggctatcag cctgactatt ttgctagaca aatgattacc
    18781 aaggaaacga aaacaattgg tgtactcgtt cctgatatca cgaatccatt cttcagtaca
    18841 ctgatgcgag gaattgagga tatcttgtat aagcagaatt tcgtcacgat attatgtaat
    18901 gctgattctg atcatcaaaa agaaatcgaa tatctagccg agctcacacg tcgaggtgtg
    18961 gatggattca ttatcgcgac ttctgctgtc tctacagatg caatcaacga aaatttgaaa
    19021 aaacaaggcc ggcccttcat tgttttggat cagaaaaaat ctgaaggatt tagtgatgcc
    19081 gtacgaacag acgattttcg cggtggttac ctagcgggca tgcacttgct tagtctagga
    19141 catcaaacga tcgcccttgt ctatcctgaa aatccaccgg aaaatgtcca cgctcggatt
    19201 gaggggttta aatctgctct tgatgtttac caaatccctc acgaccaact gatcctctta
    19261 cctacacagt tctcaaaaca aggtggctac cagatcacag cagaacttct tgattctgct
    19321 gcaactggtg tgtttgcttt aaatgatgaa ttagcttttg gattatatcg cggattagag
    19381 gaagctggaa aaagcatccc tgaagactac agcattattg gctatgataa tatagatatg
    19441 tgcgagtata tcaagccgaa actgacaacg atcgcacaac caatcttcga attgggacaa
    19501 acatctgcca agttattgtt agatcggatc caattcccag aaaaagaatg ggaagaaaaa
    19561 agattacctg tacgattaga aaaaagattt tcaactgcac ccttgaaata gctttcaagg
    19621 tgtgctatat ttttatcgaa ctattaaaac gttttactaa aacgttttaa aaaataattt
    19681 tcccatcaaa ataaatgaga tagttggagg tttgtaaaat gaacacggtt acagtaattg
    19741 gaagtattaa tatggacact acccttcgtt taaccaacat gcccaagcca ggcgaaacga
    19801 tgcatgcgca tgagattttt cacgctggcg gaggtaaagg tgccaaccaa gcagtggcag
    19861 ccaaacgttc aggcgcacgg acatctttta ttggaggcgt tggggctgac agcgaaggac
    19921 aacagttatt agatctactg acaaaagaaa acatcgatac atctgggata gctgaaatcc
    19981 aaggcgccac aaccggacaa gcaatgatta tggtagacgc agcaggagaa aatagtatct
    20041 tgatccatgc aggggcaaat aatgctttcc atgaacaaga agtcctaaaa aacaagcaac
    20101 tgattacaaa cagtgacttt attattgctc agtttgaaag tagcttagat gcgacgatcc
    20161 tagctttttc tattgcaaaa gacgcaggaa aaacaacgat cctgaatcct gcaccagctc
    20221 gtgaaacgat tcctactgaa ctacttgaaa agacagatat cattatacca aatgaaacag
    20281 aaacagaaat cattacagga atcagagtga cagatcataa cagtttggtc gcagcagccg
    20341 aaaaactcca tgaattaggt attggaacag taatcattac actaggcagt gccggcgctt
    20401 tctatcatac agaaaaagaa cacggtatcg tccctgcttt taaagtggat gccgttgaca
    20461 caacagccgc aggtgataca tttattggcg cattaagcag cacacttcag ccagacctta
    20521 gtaacttgaa agaagcgatc ctttatggaa atctagcctc ttctgttgct gtgcagtctt
    20581 atggtgcgca accttctatc ccttaccgcg aagctttaga acaatcagca gtctaaaatt
    20641 aacaatgaga ggaagaaaca aacatgaaaa aaggaaaagt aatcaattca gatatttcac
    20701 gagtaatcgc acaaatgggg cactttgata aattaagtat cggggatgca ggcatgcctg
    20761 tcccctttgg tactgaaaaa atagatttgg ctgtctcaaa cggtcttcca agttttttat
    20821 ctgttttaga aaacgtttta gaagaacttg aggtacaatc gattgaattg gcggaagaaa
    20881 taaagacaat gaacccggaa atattggaaa aaatcaaatt attgttacca gatacaccga
    20941 ttagctttat cccacatgaa gaaatgaaaa aagagttgaa taactgtcat gcatttattc
    21001 gtacaggcga aatgacacct tacgccaata ttatattagt gagtggagtg gtcttctaat
    21061 ggaaacgaca atgaatcctg aacagatggt actgtccgag cagattcata tgctgattga
    21121 acagttggga ggaaaagaca atattgattc tgtccttcca tgtgcgactc ggcttcgtat
    21181 caaattgaaa aaacagccag tagccaatgc agaaaaatta aaacagcaaa tcccttccat
    21241 taagtatttc attttactcg atagatatgc agaaataatc atggatcagg gattggctcc
    21301 tgcttacaag gaattgacgc gtcaaatcga tcattcagct ccgaaagaaa agctaaatcc
    21361 gatcgaaaaa ctatttggtt tgatatcgga aatctttacg ccgatcattc cgctgtttgc
    21421 cggttctggt atattaaaag gtctggttgt tttagcgact acgattggct ggcttagtga
    21481 aacaagcgga acttatcata ttttgtctgc tgcgtcgaat gccgtctttt attttctgcc
    21541 aatctttctt gctttttctg ctgccaaaac gtttaaagtg aatggatata ttgcggctgt
    21601 cattgctgca gcactgattg aaccgaatct gacctcttta ctttcagaca gtggcaaagc
    21661 agctactgat tttctgggga ttcctgtagt actgatgcag tatacgtcta ctgtcatgcc
    21721 ggcgatttta ggtattttct tttattcttt tgttgaaaaa ttcctcaaaa aatatattcg
    21781 cgaaaatatg caattagtat ttgtaccttt actttcatta gttatcacgg ttcctttgac
    21841 gttaatggta ttcgggcctg cgggtgttta ccttggcgaa ggtttagctg ccgcaattac
    21901 ttggatgatg gatattaacg gaccgatcag tggcgcaatc atcgctggtg gttggaatat
    21961 tttggtaata tttggtatcc aatgggctgt aaaccctgtc atgatcagta atatctctgc
    22021 ttatgggttc gaccgaatcg ttccgttgac aggagcagcc aattttggaa tggctggtgc
    22081 agctttaggt gtatttctgc gaagtaaacg ttcaaaaacc cgttcgatct ccggttctgc
    22141 ttttgcttcg attttacttg cgggagtgac cgaaccaact gtttacggaa ttgcgatccc
    22201 attgaaaaaa ccatttgttg ctgcttgcat tggtgcagct gctggtggag ctgtaatggg
    22261 atttgcccaa gtgaaagcga tcgcttttgt atttggtagt ctgaccacat tgcctgcatt
    22321 catttccggt acttttttct ggtacttagc aggactggct gttagtctag tcgtagcaat
    22381 gatcactact ctcgttagcg ggtttgatga agacctaatg tcttatgaat agttaatgaa
    22441 aaagactgaa gtatgagatg tttatcacat atttcagtct ttttcttttt aggatcatta
    22501 tttttataaa tttttgtgat actttccaaa ctttggtata atttacatat gcataaccat
    22561 ttttatcccc acttgtttat ccccaaacaa aggtggtcta tgcaataaaa tgaacgtcaa
    22621 agaaaaacga atgaatgctc gttgcttatt tacaaattgt tttatttaga atacacagac
    22681 agatttatga aaagaaaaag cttttcgaca cgattatctg ttccaaaaaa tgtctaatga
    22741 atgctgaata agacagaaaa tcaatgaaag gaaaaggact agtttattat gaaaaataaa
    22801 aaaacgatac gtttgctagc tgctacttgg ctgattgctc cgtctgtttt gtcgaccaat
    22861 accgtatttg ccgtagataa tcaaatagct gatcaaacag tcacaacaga tacgactaca
    22921 tccagctcag ctactagtga aacaacggat tcttttacag aagacccaac tggaacaact
    22981 aactcaacaa caggatcgac cgagccaact gaaattgatc cggctgatcc atcagaaaat
    23041 gaaaagccag ccgagacata ttcccttaca attgctcctt ctttgattgg taagatccaa
    23101 ctgactgctt cgacagcgga ttcccctgaa cagcaacctt tggatattac ggaaagtgga
    23161 acggtagaag gtttattagc ggaaacgaag gtaacttaca ctattacgcc agctgaaggt
    23221 gaacaaattg cttcagtcgc tgttaatggt gccaaagaag taaattatga tgcaaagacc
    23281 tttacagtag gtacgaacaa tttatctatt actgcagaat ttaaagctgg accggatact
    23341 tcaacagaga aaccaactga tcctagtgaa gaagtcccta gtacaccaga tccggaaacg
    23401 ccaaacaaac cagatgtggg taataacaac aataataatg gttcaaataa ttcaaacaat
    23461 aataacaata attcgaacag cgggaattct tcgaatcaaa caaacccatc tggatcgaat
    23521 aacaatggaa atactcagag agaaccaaac catccaaaca atggttcatc gaatggcgga
    23581 acgaacaatg cttcatctcg tccaaataca gcaaatcaaa gcggtataga gaatccatct
    23641 tctgcttctt ctgattttgt tgtaaaatca ccaattgatg ctgtattgcc tacaaataca
    23701 acgaatgtcc aacaagcaat cgttcgagaa gcattcaagc atcttggaaa accatatgta
    23761 tggggagcaa aaggtccaaa tacttttgac tgttctggtt tgacttatta cgtatatatg
    23821 aaagcaaccg gtcattatat cggtggctgg acaggcgaac agcaatatgc aggtacgcag
    23881 attccagtaa gtcaggccca acctggcgac ctcgtcttct ggggaccttc ttccggtgtt
    23941 actcaccatg taggtatcta tattgggaac ggacaattta tccatgcgcc tcaaccagga
    24001 gataaagttc gagtaacttc tatttcagat ttcacgcctg attttgctgt ccgagtaaac
    24061 ttagcaggtt tacctgctgc ttcaggttcg ctgacaggcg gctcatctat tttagatgga
    24121 ttagatggct cgttccattt cagtcaaaac caaacaactg atcagttctt gaaaaagatt
    24181 gcagatgatg cacaggaaat cgggcaaaaa gaaggaatct acgcatcagt catgatggct
    24241 caagctatcc ttgaaagtgg atctggaaac agcttattat caagcgaacc aaatcataac
    24301 ctttttggta tcaaaggatc atataaagga agcagcgtga ccttcaatac acttgaacaa
    24361 gatagttccg gacaaagcta ccaaatccgc gcacaattcc gtaaatatcc ttcttacaag
    24421 gaaagtttgg aagactatgc cgacttgatc aaaaatggac tgactggcaa tcctgatttc
    24481 tacaaaccaa cttggaagtc ggaaacaaaa gactacaaag aagcaaccaa atatttggaa
    24541 ggacgttatg ctacagatcg ccaatattca caaaaattga acgcgatcat cgaagcgtac
    24601 gacctgacaa aatatgatga accgaaaaaa gaagcagcaa caactgaaga agcaaacgct
    24661 gaaatcctca atatgtctaa gcgattcgat gtgccaatca aatggacaac agatggcgtg
    24721 ccaatcacag gattagaatt ttcaacgata aaacgcgtca acaacgcttc ttccatcttc
    24781 cgtgtcgctt caatctggga tttgtggaac aattttacgg caagacagat accagaaaca
    24841 actactcgta cagttaaagc tgcaactgtt cctgtactaa ccttgttaag tttagacaaa
    24901 tcattctggc ttgatctgcc tttaaagaaa tagaataaat taaaaagggg atgtgacact
    24961 gtcacactcc ctttttaatt tattctattt ctttatattt attttgtatt ccgttacaaa
    25021 ctcgcgtact tggttaggtt ccagagtgac atctccaaaa ctttcaagtc gctccgctcc
    25081 tggcagttct tgagtctcaa atgtgatacc gccatgattt actaatttat tcccgcgcat
    25141 ttccggtcca tcttctccaa aatttgctgt aaaaatcacg attcctgaag catctgtttt
    25201 tacttggatc gatacttttt catctgggct tgtcaattcc gcagcattta gtaaaccaga
    25261 ttgttttttc aacaaaaatg gatgatcgat gccgcccact atcattgatt ggcgaaaatc
    25321 actggcaaag acctcaccta attttttagg acgttggaag tcaaatgctg tgccacttac
    25381 tgaaactact tctcctgtag gcgttgtatc tttattgagc gtagcaaata atcggctatt
    25441 caaccaaagt atatgttcgt cgattgactg tgctggatca ccggttaaat tgaaatagac
    25501 atgatttgta ggattgaata ctgttttgcg gtcactgatc ccttggaccg tcactcgcca
    25561 tgtatcatcg ttcgtcagcg tataccgtac ttctacatct aaagttccag gaaatccgtt
    25621 ttccatatct gggctagtct gatagaaaat gaccgatgct tcgttttcac cattgatgac
    25681 tgtataatgc cactgcttgg cttcaaagct aggtgcacca ccatgtaaac aatgaccagt
    25741 gtccgggtct gtttttactt cgaacgtttc atcatctaat ttgaatgtgc cattcgtaat
    25801 ccgtccagcc atacgtccga ttgaagcacc gatataaggg tctttttcta gatattcttt
    25861 tgcttcatca aaacctaaga tcaactctcg tttttcatta tcgattattg tttgaaaatg
    25921 aacaacccgt gccccaagat cactgacaga caaagatgtc ccattttgat tcgtgatcgt
    25981 aatcaagtga tagttttcgc caaaattttg ttcattgatt tccatgttcc ttgcaccctc
    26041 ttttctttca tttgcactga ttccagtata catggaaacc gttttcttaa cagcttattc
    26101 cgtgagtaac gagaaaaaat ttactatatt ctaataaaag accgtgaaaa aaaggttgtg
    26161 acaaaaatat tgtcacaacc tctttttccg aacaaacggt gttcaaaagc ttgcatctgc
    26221 ggtaataagc ccaaccaaac gaaaaatatg aggagctatt tttgcttggc tgttcttatt
    26281 acttgatgca ggacagcttt ttcacaatct ctttttcaat acgattccag tatataacca
    26341 tgtcgtcagt acatagtaga tgaaatacaa tacgaagaat atcgtaaacg gaacccaaat
    26401 atctttgtat ggattttgca tgaatagacc aaacatttgt aatccgaata agacatggac
    26461 tacacctaag attcctggtg ctaagaataa cacgcctact tctctacgga tcgattgttt
    26521 aagcaacccg cgtcgtgttc ctatcttttc aagcatttca taacgagcaa tgtcactctt
    26581 gcttcctgac aagatcttga acatcaaaca gctagcaagc atggtcaaga aagctaaacc
    26641 taggaagaat cccataaatt caaaaccaga gaatagacca ttatacactt catacatttc
    26701 atatttctgg ttgctgtact ctactttttg caaagatgga ttgttcattt tattttgagc
    26761 aacaagtgtt tcgattggtt tcaggtttgc ttggaagtct tttacctgaa ctagctgtaa
    26821 agtggattgt ggcaagttca gttgatcaaa ttctgattga ctgactacct tgatttcttt
    26881 ttcttgctgt tccggcaatt catagcttcg aagttcctct tgtacagtca gttcattcat
    26941 ttttttagca ggaacttttt ctttcgtgat cttcggcggt gttccatctt catgttttat
    27001 catgatcaat ggttgagccg caaattcact tgcattatag taaatcgttc ccgcatcttc
    27061 tttttgagta taagaagcag aaagtgttgg tgaaagttct ttgatttttt gctgatcttc
    27121 tgcccgagga ttattcatca ctagatcata tccagaattg ttttctgtca agtctgcaat
    27181 ttcattttta aaacctaatc ctaccgtcaa agcgcccagt gccaaggcaa acagcatagc
    27241 gaccattgag agcatttgtg tatattcacg aatacgaaag tttaactgtg acaatgtgaa
    27301 attgttcaat tttttcaatg aaatctgttc tgatttcttt agtaatgata aaaagaaaat
    27361 gatcacagaa tggaaaatga aataagttcc taatacgatc gtaactaagg ctacccctat
    27421 accaaagact tgataggtcc ccacgtgttt catcatatag taaccgcagc ctagaaaagc
    27481 aattcccagt actacttcaa ggaaaaacaa gaatttatgc tgcttgattc ttgttggtgt
    27541 ctgatccgca cgaatcaatg ctaaaatcgg ttttttgaca atcgaaaaag cattgacaac
    27601 agcagctaat aaaaacaaaa cagcaaaaaa caaaacagtg atcaacaacc cgtgaatact
    27661 aaaaggtgta aaatgcgaaa tattgagatc taactgatcc actaataatc ggtggacgat
    27721 acttgtcaat cctacaccga cagctgaacc cactaaagta gctgcgatcc ctactaagaa
    27781 tgtttcagaa aaaatcatct gtgcgatttt tctgcctttt gctcccagca tcataaacat
    27841 tgcatagtct ttttgccgca tcgtcatcaa aaaggaattt gcatataaaa tataaacgaa
    27901 tgtgatgatc cctagtaaaa cagaaccgaa tcggaaaatg ataactgtag ccgcaacgat
    27961 cgtattactt ttcaaaaaag cttcgttgct tgccattgat tcaaacatgt aaaaaatagc
    28021 agatgccata actaatcctg agaataacac caagtagtct cttaaacggc tcttgatccc
    28081 agtaagtgat aatttccaaa gcatagtcaa tctcccccta ctgttccaag tttccaagaa
    28141 tagatagtat ttctcgatag aaggaatcac ggctttgatc tccacgtttc acttcttgat
    28201 ggataccgcc atccttgatg aataaaatac gttggcaata acttgcagaa aacggatcat
    28261 gcgtcacaag aagaatcgat acatgatctt ttgtattcaa ttcgtccatc gtatctaaaa
    28321 gatcccgggc acttttggaa tccagcgcac ctgtaggctc atcccctaat agaatcgttg
    28381 gttgtgtgat caatgcacgt gcggcggcga cccgttgttt ttgtccaccg gaaatctcag
    28441 aaggatagct ttccaaaata tgagagatgg ataaacgttc tgcaattttt tggacacgtt
    28501 gcttgatttc ttttggtctg acgccttgta aagaaagagg aacagcgata ttttcttgtg
    28561 ctgtcaagct ttctaataaa ttgaaatctt ggaaaataaa gccaatctca ttcgcacgaa
    28621 aatctgctag ttgatttcct tttaatgtgg tcacatcttt ttgattgatt cggatatgcc
    28681 catctgttgg tttatctaat gtggataaaa tattcaaaag tgtcgactta ccagaaccag
    28741 aagcgcccat gatcccaata aattcgcctt tttctacatc gaagctgatg ccgcttaatg
    28801 cttgcgtctt tttctcattg ttttttccgt atacttttgt tacattctgt acttcgacta
    28861 ttttgttcat ttctttccct accctttaat tagtttttcc agtgctgttt tcggcacgtc
    28921 ttttttttcg acttcttcat acgaagtgac acctttattt ttgttccagg tgatttttaa
    28981 atatgctcct ttacgaagcg gtcgagtcaa ttgtcccttg aagtccagtt ctttttcctt
    29041 accctctttc gtatagccag gcaggatata tctatagttg actgtttctt gtccagagtc
    29101 tgattttgaa actgtttttg taccatctgt cgtaatttgt acatagtatt cttctccacc
    29161 cataactacg taatctgcga ttttcacccc tgcaagtacc acaacgatga aaacaagtag
    29221 accgatcaga agttttttca ctttttcccc ttctctctta ctttatatac tgattataga
    29281 aaaaagcgaa caattctgcc ttcgaatcaa ctatcattct tctttcaatt ttgtcatttt
    29341 ctttaaaaca actaaaaaaa gagttcaaga caataatccg tcctaaactc cttcttccta
    29401 tcttacaaaa agtatgtcac ttacggtctt tcatgtcgcc tgtattgata acaggttgcg
    29461 ttcctttgtc tgtaatgatt gagacaagta aattattgat caattggact ttccgatcat
    29521 cggtgaaatt gatttcttgt ccatcttcga tttgctctaa ggccatttga gtgattgtga
    29581 cagctccttc aacaatggtt tgtcgagcag acaaaatggc ttttgcttgt tgacgttgga
    29641 gcatggcact agcgatttct gttgcatagg ctaaatggtt caagcgtgtt tcgattactt
    29701 cgacacctgc tactgctaaa cgctcttgca gctctttttg caactcgtca gacactgccg
    29761 tcgtgtttcc tcgaagcgtc aaatcattct cattgaatgt atcatacggg tattggctag
    29821 cgatatgccg aatggctgtc tcactttgga tttctacaaa atcttgataa tacgctacat
    29881 cgaataatgc ctttgctgta tcaacgactt taaagactac tactgctgag atttcgatgg
    29941 ggtttccatc caaatcattg acttttaaaa cagaactatt gaaattccga actttcaacg
    30001 aaacagtcat tttttgtgcc aaaggtatcg taaggaaaaa gccattttct cgaatcgtac
    30061 ctaaatattg tccaaaaaac aaaattactt ttgcttgatt aggactgact acagttgccg
    30121 aacttaacaa caataacgtg atcaaccaaa gaaaaatact taaaaaaagt gcccaaactt
    30181 tcgcttgcca catacctaaa tagaagaaaa acaatccaac taaagctaat acgaccaatc
    30241 cgacaatccc tgcataacca ttgatataaa atgctctttt ttctctcatg ctacttcctg
    30301 cgctccttta tccttcaata aatatctaat gttacttttt taattttttt ttcgaatacg
    30361 cgccattaca ccttctagtt ctaactcttt gtcttcagtg atgtaccaac cgcttgcctg
    30421 tgtccctccg gctgcctgcg gtaaataatc caattttgtt cgaacccagt gcttttcttc
    30481 tgtaaaatag tcttctacca agacctctag atccatccct gctgaaagag gcaaatcttc
    30541 ccgaacagat ttaaagataa atcgttcctt cttcccatca tacttcaata ttcctgtatc
    30601 gataatcggt aaagaataat aatccagctc tttcttcacc gtcaataaat gtttgaaaac
    30661 ttcatggtac acttgctcta attggcgacc ttctgaattg ttcgggtaat taaccatcac
    30721 tttttctttg tctggatcat agaaggtttg gcttagttga gtgatataag tatgtaatct
    30781 ttccatttct gtttttaact tttctctttg accttcttct accttcatat actctttcac
    30841 cctgttttct ttttagatat tttctttctt attttactat agttatgtgc gttaagcgat
    30901 gaaaaacagc aaagattgga aggatctttg ctgttttttc ttaaaatatc cagttgagta
    30961 caggatcaat ctgtttcatt gtcctcttga tccgtcgtaa tattgatcga tgtgtcttta
    31021 gcagtgatct ttagcttttt ctgtccttgt tctaacaaag tatttgttat tggctggtag
    31081 tctttttcaa tataagattc accagacgtt tggatagtat agccactgtt ttccatctca
    31141 tgcaaagata aattgccacc gtttaggatg acttttcctt ctttgctgat caaactgtct
    31201 tctagatgga agttgctgtt ttcacctgtg acgatcaagt tatccacatt ggcctcatct
    31261 aatcgaagtg agcctaattc atctttcgca gttattgttc ctcctgtaaa ttcattgaac
    31321 gatacccagc catcatgtgt cttagtattc acttttttga ttgatacttc gttgatatca
    31381 attgcactat tattcccttc gattgtcaat tcagataatt catgatctgc agggacaaca
    31441 atagtgattt gtgcacttgt gtcttctaaa ctaatgattg cacctgttga ttttttgact
    31501 gcattcacag tcaacacgcc atttttctcc tttatttctg gcttttctgt cttatcataa
    31561 gtcgttttta tctcaaaatc caatccgcgt atgaccgaaa ctgtttgatt ttctgcatgg
    31621 atgacgattt tgtttgcatc cttgattttt tttgtctcag taaccatatc gatcaaccga
    31681 ggaccattat cccaagtcag actcttttga gcgcctaatg tataagttgc acctgctagg
    31741 aggccaccag taagcatcag accaataccg gtaatcaaaa aatgtttcaa tctcaattta
    31801 tgtgtcctcc ttcaaacgcc cgctgaatgc agatctcttt ttgtaataaa acgtcgtccc
    31861 atccatttaa caaagttcat catgacataa aaaagccctt ttgttccttt cagcaacagt
    31921 ggcgaaataa atactgcgct tcctgtggcg aacagaccga caccgataaa gaataatcca
    31981 ctcagaatcg attgtccaaa cacactgatt cctgagatga tcatgaatag gccaacacct
    32041 aagatactga tacataaaac atagatacta aaaatgagcg ctagtaaagc aaccaatagt
    32101 cccccaataa ccaatataaa ccccagtgct agtggaatga ggacaggaga agcaaataaa
    32161 ccgataatga ttaaccaaat caggcgaatt tgtctttttg gatttttcac taactcgttt
    32221 tctgcttgtt cttcttctga aaagtaactg atttttaatt ttaaggcaaa ctgctttggt
    32281 gtaccatatt catcaatgat ctgatctaat gtttttccag aatctaaaaa atattcctca
    32341 taatactgga tcaaatcaaa acgatcgtct tcaggaattt cttgaagatg atccacaact
    32401 tcattcaaat attcattcat ttgtgctctc ctccaataac cggtctaaag aatctcgaaa
    32461 aacaatccat tcatcaataa tttcttctaa atgacgactt cccattggtg tcaacgagta
    32521 atatcgacgg tttctgcctt caaaaggttc atcataggtc tcaagatatc cttctttttt
    32581 caatcttctc aaaaccggat atagtgtaga ttcactgaca gagagacttt cttgtacgat
    32641 ttttgttaag gcatacccat acatatcttc cttgcttaat gctgctagca cagtaccatc
    32701 aagaagaaca gttggaatct ggatgtgcat tacgtcacct tctattctca agtgtaatat
    32761 tttatttcaa tctatattat acaacatata atatagtttc aaaatcagtt tgtcaaatac
    32821 aaaaaaactg actaaaagat ttgatccttt ttagtcagca aaaaatggct tctctttgat
    32881 ttttattata acagtggcat aatccaattt gtccacatcc agctcagtgg catgataacg
    32941 atcattgcag gtatcatgtc agctactggg aacattttca cgttgatcat tctaaaaccg
    33001 gtagcgacca tcaagaatcc gccgcaagct ttaaaatcta agatcatgtc tggtgtagtc
    33061 aatggataaa taaattttgc caataagaac aaaatataaa aaataatgaa ttggggaatt
    33121 gctatcacag aaacgacata ccctaaatta cacgcaaaaa tagctgctgt gaaaaaatca
    33181 aggacagatt tagaaatcaa aatcgtactg tctcctgtca tcccagaatc taaacttcca
    33241 tagatccccg taccacttgc acaaaataga acaatgaccg tcactaatgt gctgataaat
    33301 tcatcgtggg tcataccaag cttttcacta gggaagattt tagaaatagg tacttgcatc
    33361 agcgcagccc ccttatttat cccattacct aaatgaacaa tcaaaccaat cgctgttcct
    33421 atcactaaag caaaaataac agccggcata tttttcatcg gcgcaatgga atagattccc
    33481 atccccatcg aacacacacc aaaaaccatc gtcagctgtg ctttaaaatg ttcacttaat
    33541 ttatttccta ctaatccgcc aattagtccg ccaaacaaaa cggaacctac attgatcaaa
    33601 actccagttg gcatcaaaaa actcctctct ttttctacga agcgaaaaaa atcagaaaaa
    33661 tttctgtaat tattgctttc gctgcaagaa agattgtaac agacagagaa gattacacca
    33721 attcattttt tgtgttaaag tattctttat tggaataagt aggaggaata tgatggatat
    33781 ccataaattc gaagtttttc ttgatttagc cgaaacaatg aatttcacca aaactgctga
    33841 tcgacaattc accacccaag gaaatatttc taaacagatt ctttcgttag aaacagaatt
    33901 agacgtcaaa ttattcgaac gcgcccatag aaaaatcgaa ttgaccgaag ctggaagctt
    33961 attgctccca tatgtaaaaa atgtcgtaga acaataccat gctatgcaag aagtgcttga
    34021 aacctatacg aaagacaaca atctttcatt aaatatctta acgataccga caatgatcaa
    34081 ttataaaggt ttttccaaaa tcaccgagtt tttgaaacgc catcctgaat ttacagtcca
    34141 gttgaaagaa gtggaaagtg tcgaattaac ttacaatgac gccaatgaac atagtgacac
    34201 gatccacttt gctagaagtt tccaggcccc accaagttcg attgagtggt tgcctactga
    34261 aacagacgat tttgtagcag ttctacctaa aaatcatcct ttagctagta aaaagaaatt
    34321 ggatctaagc gaactgaaaa aggagaactt tttgctcctt ggccccacta cgaacttgta
    34381 tcaacctgta ctagatctgt gtcgcgaagc tggatttgaa ccaaaaatct cttataaagg
    34441 agcaagaatc gacttgatca tcaatatgat tgcaaatgac atggggattg ctattgttat
    34501 ggaaaaaaca gtcaagaatc tattaaaaga aaatactgtg attctcccta tttctccaac
    34561 aacagagagt tatcttgcat ttactcgtaa agtcggtgag cattctttgg cttcagatac
    34621 attttgggag tatttaaatc aagaagaaaa ctaagcccta cattttctgt ccatctatcc
    34681 acccaaaaaa agagagcgac gcatgacttt cgctctcttt cttgcatcat gtccttgttt
    34741 caatgacacc tctaacattt ctcgtagttc aataagaaag cagcgatttt tatgccaaaa
    34801 gaaccgtaag aacatttccc taatcacaaa aaaacgacta aaaaccccta aaaaccttca
    34861 atagaatttt taggggtttt attctttttc ctattgttta cttaaccgta tcattgctgt
    34921 cgttcctact ccttcgatac tgctataatc aatacgtcca tcatgcagtt ctagaatttt
    34981 tttaaccagt gaaagtccta gcccattccc agaaatttga cgacttgtat ctccttgata
    35041 aaatttttca aacattcgat ctctggtctc ttcattcatt cctacacctg aatcggtcac
    35101 ttccagtaca atctctgtgg ctgtctcaaa tagtttgata tgaatctgtc cattgatttg
    35161 attgtacttt atggcgttat ccatgatatt cagccacact tggtacaaaa actcttcatt
    35221 gcctgtgtaa tttactgcag caagttcgat atccaattct agctgttctt tctcccattt
    35281 aggctgaaaa aaaacgatta cttctctgat ttgttcatct aatcgatact ctttcttttc
    35341 cagttggata cgctggtttt ccagtttgtt caattttaag atgttctctg ttaattgtga
    35401 aagctgcgtg atactttcga taatccgata taaaaatatt tggcgttctt cgtcagagag
    35461 atttggggct tgtaacaatt gaacatatcc ttggattgtt gctagtggtg ttttgaattc
    35521 atgggataca ctggacacaa agtcattcct cagtgtctcg atactattga gttcttggac
    35581 cataacgttg aaatctttat acagttgctg tacttctgct actttctgct gttcatccat
    35641 tcgaatcgaa aaatctcccg ttgctaccag actcatattt gtccctagcg cactgattgg
    35701 atgcaggata cgccgcccaa caaaaatcga tattcctgta cccacgacca tactgaaccc
    35761 cgcaaagacg aataacgggt ataacgttgg ttttccagcc gattcagcag gcgagataga
    35821 atgtcgagac aataagaaaa taaaaccgag aaaacacagc agtgtcacta agatcgaaat
    35881 gaagacaata ctagcaaaat agatccataa ttgtgaaaat agcttttttt tcatctttaa
    35941 ataaccgcct tatagcctag tccccgtact gtgatgattt gaaaatcttc cgtcttttca
    36001 aaacgggtcc ttaaacgttt gatatgtaca tcgatcgttc gttcttctgt attcgtatct
    36061 aatccccaaa tatcatccat caactgttgg cgagtgaaga tcttgttggg ataagacagt
    36121 aatttataca gaagcagaaa ttctttttga ggcagaatca acgaatgctg atcttgtaac
    36181 actgtataag catctagctc taattttgtt tgtccgatta tcaagctact gctgtaattg
    36241 atctttgaac gtcgaagcaa tgcttccaca cgtaggatca tctcattgac atcgatgggt
    36301 tttgtcatat aatcatctgc tcctaatttg aacccgcgtt tcttatcctc aaaatcggcc
    36361 tttgccgtaa tcattagaac aggtgtgtca aatctggtgc tacgtaatga gtctagcaag
    36421 tcatagccat ccattttcgg catcatgata tctgtaatga ttaattctat acgtgtcgct
    36481 tctagttttt tcagcgcatc ctctccattc aatgcggtga tgactgaaaa gcccactcgt
    36541 tccaacacag aacggtaaag caactgtaat tgcggatcat cttctactac taaaatacga
    36601 ttcattgatc tccctctgtt ttttgaaaaa tttctagatt ttttcttgta gttcatcttc
    36661 agttaacatt tatttcgtat actacgatca ttgttctgat atacttatta gtataccgaa
    36721 tatttcaata cgggagaagg gtgattatgc ttcaattaaa agatataaaa aaatattata
    36781 aagtcggcga aaccaccacc aaagcattgg atggtgtgtc tgtcgctttc agacaaaaag
    36841 aattcgtagc gattttaggt cctagtggtt ctggtaaaac gactatgctg aatgtgattg
    36901 gtggactaga taattatgat tccggtgata tggtaatcaa cggaaagtct actaaagact
    36961 ttaaggacag tgactgggat gcctaccgaa acaactctat cggtttcatt tttcaaagtt
    37021 acaacttgat tggtcatcta ggcattattg aaaacgttga acttgggatg acattaagtg
    37081 gtgtttctaa agacgaaaaa cgaaaacggg cagaagatgc attgcaccga gtcggcttga
    37141 caaatcatat gcataaaaag ccaaatcagt tatctggtgg acaaatgcag cgagtggcca
    37201 tcgctcgagc actagccaat gacccagata tccttttgtg cgatgaacca accggagcgt
    37261 tagatacaga aacaagtatc cagatcatgg aattgatcca agagttatcg aaagaaaaat
    37321 tagtgatcat ggtcacacat aatcctgaac tggcccatca atacgcagat cgaatcattg
    37381 aattttctga tggtaagatc ttaacagatt cccatcctca catcgaacga ccaaaagatg
    37441 atcaatttaa cttacgcaga acaaagatga gtttttggac agcgttaaag ctttccttca
    37501 acaatatccg aacaaaaaaa ggacgtactt tcttgacctc ctttgcttcc agtattggga
    37561 tcatcggaat cgcgatcgtt ctgtcccttt cttctggatt ccaaaaacaa atcgataaca
    37621 cgcaagctga aacgatggcg aaatttccga ttactatttc taaggtaacg actaaccaga
    37681 ctcgtgacga tgccggattg ggtgcaagca aagcggatta tccagacagc aaaacgatta
    37741 ctgcaaaagt cagcgaagaa gataaagcac agcacacgaa taagattgat caaacatacg
    37801 tcgattacgt aacagacatc gatcctaact taagtaataa tatcggattt acccgtacta
    37861 caggaatcaa tcttttacgt gatgtaaatg gcaaagtaca gcctgtcagc ttctcgaatc
    37921 aaaatcctga tacggaatct ctttctttat ctagtacgat gtcagcaatg acaggtgtgg
    37981 gagtgtcaag tttccctaca cagcttgaca ctagcaaaga aaacttttta aaagacaatt
    38041 attctttact tgccggaagt tatccagcct cagcaacaga tgtcgtgctg atcgtagacg
    38101 gcaataataa tactaatatc aacgcattga agaatcttgg atttgatgtg aaagaagacg
    38161 aaaaacttga ttttgatgat atcgttggta caacattcaa attagtaaac aacaatactt
    38221 actatacaaa acttccaact gggaatttta tccctaatac agattatgat gcgatgtatc
    38281 agaatgcttc tgatgaattg aagatctcgg ggatcctacg cgtgaaatct tcttctacaa
    38341 tgaatctctt atctccagga atcgcttata gcgatcaatt aacgactcaa atcgtcaacg
    38401 aaaacaaaga atcagaaatc gtcaaagctc aaagagacag tggtgtaaat gtcttaacta
    38461 ctgaaaaagt tgacgaaaat gccaaacaaa cattgcttag ttatttaggt ggagacagct
    38521 tgccttcaag tatcatgatt tatccgaaca attttgaaga caaagaaaag atcttggatt
    38581 atctagatga ttacaataaa ggcaaatcag atgaagataa aatcatttat actgatttag
    38641 ctggaacgat gactgaactg actggcggat tgatggatgc tatcacttat gtccttattg
    38701 cttttgcagg gatctcttta gtaacaagta tgatcatgat cagtatcatt acttatacat
    38761 ctgtgattga acggacaaaa gaaatcggtg tactaaaagc tttaggtgca cgtaaaaaag
    38821 acatcactcg cgtatttgac gcagaaacgt gtattttagg tatttcttct gggatattag
    38881 gtgtgtttat tgcatggcta gctacgttcc ctatcaatag catcttatac aacatgacgg
    38941 atctaaaaaa tgttgctcaa ctaaatcctg tccatgcgat tattctagtg atcgtctcta
    39001 ctatattgac gatgcttggc ggtcatcttc ctgcacgaat ggcagcgaaa aaagacgccg
    39061 ctatcgcttt gcgtgcagaa taaggttgtg acaaccacat ttagatttga gtcataagag
    39121 cgctgaaata gtagaaaaca gcaagaaacg aggaagtaat atgttccttg tttcgcgctg
    39181 ttttttttaa ttatttttta taattctggt atacttttct ttcctttgta ccacaactct
    39241 tctggcgtat acctgtcttt tccatttatg aatactgttc catctgcttc aatcgttact
    39301 tcagtcccaa tccacatatt acgagaaagt gcgagaagat tttttctttt tcctcttcct
    39361 ttttttccaa attgcaaggc atcggttatc tcttctgtcc aatgagattt cacgtatttt
    39421 acttctccgt tttcaaatgt aactttcaaa atattcgcta tattttccac ttttactgct
    39481 ttaggaaatg actcctccat ttttttcact ttccttcctc caacactaag tatgcgcttt
    39541 cttttagtga taagtatatc atagtttatt ctattatcaa aaaaacgcat ccattccatt
    39601 gtagaatact ttaccgaaca agttgaattg ttttcctctt tttcaccttt tactatagat
    39661 aggtaaagac aactaaggag gaatctctca tgacagatca accgtatgat ttaagaaaag
    39721 tcatcgaaga actaaaaaca cttccaggtc aatatcacga aacagatatt gaaatcgatc
    39781 ctgaagcaga attatctggt gtttaccgtt atatcggtgc tggcggtaca gtaaaacgtc
    39841 cgactcaaga aggaccagct atgactttca acaatatcaa aggtttcccc ggcacacgtg
    39901 tgaacatcgg aacaatggct agtcgtaaac gtgtaggtga aattttgcac catgattatc
    39961 gaacacttgg tcgtttcttg aaagacgctg tagaaaatcc aatcaaacct gtgaaagtag
    40021 acaaagcaga cgctccagca caagaagtcg ttcatctagc taccgatgca gacttcgata
    40081 ttcgcaacat tttgcctgca cctacaaata ctcctgaaga tgctggtcca tacatcacaa
    40141 tgggtgtagt cttaggatca gatccagaaa aaacaatgag cgatgtaacg atccatcgta
    40201 tggttctaga agataaagat acgatcggta tgtacatcat gcctggcgga cgccacattg
    40261 gtcacttcca aaaacaattc gaagccttga ataaaccaat gcctatcact atcaatattg
    40321 gtttagatcc agcaattgcc attggcacga ctttcgaacc acctaccact ccacttggct
    40381 acaatgaact atggtgtgca ggagctttgc gtaacgaacc tattcaaatc gttgatggta
    40441 tagcagtcga tgaagtgggt attgctcgtg ctgaattcat cattgaagca gaaatcatgc
    40501 caaatgaaac aatgcaagaa gatatcaaca cgaactcagg aaaagcaatg ccagaatttc
    40561 ctggctatac tggggatgct aatccagctg tacaagtagt gaaagtcaaa gcagttactc
    40621 accgaaaaga caatccaatc atgcaaacaa cgattggtcc cagtgaagaa cacgtatcta
    40681 tggccggtat tcctacagaa gcaagtattc ttgccttggt cgataaagct attccaggaa
    40741 aagtagtaaa tgtctacaat cctcctgcag gtggtggtaa attgatgacc atcatgcaaa
    40801 tccgtaaaga aaacgaagcg gatgaaggta ttcagcgtca agcagctatt cttgctttat
    40861 cagccttcaa agaattgaag acagtcattt tagtcgatga agatgttgat attttcgaca
    40921 tgaacgatgt agtttggact ttcaacactc gtttccaagg agataaagat atcatcgtga
    40981 tcccaggaat gaggaaccat ccattggacc catcagaatt accagaatat aatcctggct
    41041 ctattcgttt ccgtgggata agtgcaaaaa ctatctttga cggtacagtt cctttcgata
    41101 tgaaagaaca ttttgtacgt gcaaaattcc aagaagtaga agattgggaa aaatatttga
    41161 aataatgaat gatctgcaac tgttttgaca tcataaaaag gagtgcggaa aatggaacaa
    41221 ttggcacaac ggaagaaaaa aatcgtgatt ggtgtaagtg gcgcttcagg caccatttat
    41281 gccatcaacc tagtgaagaa actaaaagaa tatccgatga tagaaacaca cggaatcatc
    41341 agtccatggg caaaacagaa cttaaagctg gaatccgact tatcactaac tgaattcgaa
    41401 agcctgtttg attaccatta ttcaaacaaa gatttaggtg ctgctgtcgc cagcggatca
    41461 tttttgaccg atggaatgat catcgtaccg gcaagtatga aaacagtcgc aggtatctct
    41521 gttggtttag gcgacaacct gattgcacgt gctgctgatg taacaatgaa ggagcaacga
    41581 aagttagtca ttgttcctcg tgaaacgcca ttaaatgcca ttcacttgga aaacttgact
    41641 aaattagcac gactaggtgt gcaaatcata ccaccaattc ctgcttttta taatcagcct
    41701 caaacgatcc aagatcttgt taatcaccat acaatgaaac ttttggatca atttgaaatt
    41761 gtaaatgagc attcaaaacg ttggcagggt gattaatatg gaaaaagaaa cgatagttga
    41821 agtaaccaaa ctaggtttca cgatccaagc tgctgttaaa gtaatcggta aagacttatt
    41881 gatcacttta acaggcggag atacaccaca tatcggaaca gtcactacat tttctcgtga
    41941 tacagagatg caaactgtac gtttccctag ccacgatggt cggttccata aagacgatgt
    42001 cttagccgaa aaaatagctg tgatcatcaa aactgccgtt ccgggaagtt gtacgatcac
    42061 ttccggtatc catgttgacc atatctccaa tgcacaaatc aacgcctctt ttccgatgtc
    42121 agaagagtta ggttacaaga ttttagattg gttggaaaaa atagatttcc atatcaaaga
    42181 accagtttat tataaagatg gtgaaaaacc attatgaaag aagaggttgt gaaatcagcg
    42241 ttatgacctg agtatgagag aaaaaaatgg aaaaatagct tctcatattt tgccgttttt
    42301 gagctaatac cgagggacag ctgattgaac gccgtttatc aagaggttgt gataaagccg
    42361 cttcgtttcg agtagtaaga aaaattttgt aaacatgggt ttccacattt ttgcataaat
    42421 ttgacttact accgagaaac tggcttttga acaccgttta tcaagaggtt gtgacacgtc
    42481 ttttgtcaca acctctttta tttttgcttc actgtaactg ctacaatatc accgatttct
    42541 tttccgatgt ttttacgaat atcttttcgt agtccaagga tgtagcagac tgaaccatct
    42601 ggatttttca cgcccatatt cacgatactg ccttcatacg gcacattgtc aaaagttgct
    42661 tggactttaa ctcttccagc accgaattct tttcttatat catacggaaa aataatatat
    42721 gccccgccct tgccgacttc ggaagcatag atttttgatt caaaagaata aatttccctc
    42781 attttatttc tcctattttt tacatctagt atagcatagc aaaaaaaaga aggaactgtg
    42841 acaaaagtct tgtcgcagtt ccttttttcg aataaacggt gttctcctgc caactcctcg
    42901 gtcacaagtc acaatagtca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    42961 ctcagagtta aacggcaggc tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc
    43021 tacggcacta agcccaatca gatgaaaaat atggagtgct atttttatct gattgttctt
    43081 actgcttgat gcagagcagc tttttcacaa cctcctacat ggtgttcaaa atattattcg
    43141 ccttttgctt ctttcactaa ttttgcgaat gcttgttgtt ggaattcagc agctaaggct
    43201 ttcgtacgtt gttcttgctc tgctttgctc atttttggat tttcagtcat tgcttttacc
    43261 ataccgtctt gaacataagc ttttgcttca ctttccatcg tactttgctg tttcttcact
    43321 aattcagcta acttggctgc ttggtccgta cttaattcta cccatgtctc tgggattttg
    43381 aatgtattca ctcggctgtc atattcgtga ttgttatcag caatattgaa ccagaattta
    43441 tacatgtcgt ttttgtattc ccaataagta gttttcgtca ctttttcagc atcgccgtct
    43501 gtcttttcta ctttatttgt ttcgttatcc gtaccagttg cttttggttt ttcttgtttt
    43561 tcatttgttc ggtaaagata cactttttct tttccattcc ctaaagcttt atacaaaagc
    43621 atatctgctc cttgggaatc agcactggaa acaatttttt ctgtttttgt ttccgttact
    43681 ttttccattc caaagtgatt ggacaaatta gcgacgatca aacttagtga agcgacgaag
    43741 attagtccga aaatcaaaga taaaaaggtt tgccatgttt ttttagcgaa cacgttcgtt
    43801 aaagcaaaag caagcaagct gacaatcaaa actccaataa tcatgcatta tcccccgctt
    43861 tcattagaac aggttctttt ttcttcaata aaaaggcaat aataaagcca agaatcccaa
    43921 agatgatcgc tacgataaac gcggcatggt aacctgacag tgtggcattg attgcctgat
    43981 ctttataagc taatggcgta ttatccaata cagatttgct tgggatattt gcttttgtca
    44041 cattcgtcaa cacactgatc aaaatagctg taccaatcga gcttgcgatt tgacggaatg
    44101 tattatttac cgctgtccca tgactgatca agttatttgg caatgcattc ataccagaag
    44161 tcgttaccgg catcatgacc atggagatcc caaacatacg gatcgcataa aggatcacga
    44221 tatataaaac tggagtcgtt tttgtcaaga aagcaaatgg tgcagttgcc gctgtcaaaa
    44281 tgaacatccc tgtgatggct aaacgtttcg cgccgtattt atcgaaaatc gcacccgtca
    44341 caggcatcat agcccccatg atcaatgcac ccggcaacaa catcaagcca gaatggaatg
    44401 ctgattctcc acgaatattt tgaatataca atggtacgac catttctgcc cctaccatcg
    44461 ccatatttgt tacaccagct aaaacagctg caatcgtgaa tgtagatgat ttgaatacac
    44521 gaagttccaa gaatggatgt tccaaatgta attgacgcca tgagaacaag gcgatgaaaa
    44581 tcacaccaac aactaagaaa ccaattactt tcaagcttcc ccagccgtca tctcctacac
    44641 tagaaaatcc gtaaagtaaa ctaccgaatc caatcgttga taaaatagcg gataaaatat
    44701 caatgctagg atttgacaat ggaataacac ttttcattag gaagaatgct aaaaccaaga
    44761 caagaacaac gattggcagt accataccaa acaagtcacg ccaatggtag ttatcaatga
    44821 tataaccaga taatgtaggg cctaaagcag gagcaagtcc aataacgatc cctgacatcc
    44881 ccattgctga tcctcttttt tctggcggga aaatagatag catgatattt tgcaataatg
    44941 gcatcgatac tcccacaccg caagcttgga tcaaacgacc gattagtaat gtactaaaat
    45001 taggtgctgt aaagcaagta atcgttccga tcaaaaagat cgtcatggca gaaagatata
    45061 aatttcttga actgaattta ttgatcagcc atgctgttat tgggatcatg atcccgttga
    45121 ctagcaagaa accagtcgtt aaccattgca catcagacgc tgaaatatcg aaagctttca
    45181 tcaatgtagg aaaagctgtc gtcaataagg tctgattcaa aacggtacaa aatgtaccaa
    45241 ttaataagac aacaactaaa agtgtccgat tatatggttt tccgtaaata tctacgggtt
    45301 ggttgttact catatttctc cttctttctg aattttttct gaaactacta gaatacttta
    45361 gtcctaaatt cgctatttgt caacctactt gacacataag ttaatacgga tataattaag
    45421 ggaagcaaga ggacaaaagg agtgacaaaa atggttgggg cttcccttaa acgtttgttg
    45481 gaaaatgatc aattggttgt tggcatcagt gaattaagtg atatcgtagg tgtgtctcca
    45541 cgtcaattaa ggtattggga acaaaaggga ttcattcgtt cgatcgaagc tgatgcgaat
    45601 tgtcctagaa aatatcgttt acctacagtc ataaaagtag aactgatcaa aaaatattta
    45661 gatgacggat ttactttgtc aaaagctgca gaaaaggcag aaatccgcca aaagaaaatg
    45721 caccatgtcc gtaaagtttt ttcaaaagca atcaaagata tcgaattgat cgacgaacgc
    45781 tatacgattt tacaaatcgg taacttcaaa gaagagaatg ctaagatgta catcattcat
    45841 gacaacgaga cagacgaatt aacttacaag ctccttcctg tcgatgaaaa agctgactac
    45901 gaacagttgt taaaagaact ctaaacgcca ctaatctcct aagtaccaag ctttttaata
    45961 agtagatgct acataaatac ttgaaaaaag gcctgtttga acataagaat atccgaacgg
    46021 tgatctgcgt ttctttcata tagaaaggaa ttctccatca ctgttcggat atttttttat
    46081 ttttttcggt tactttcttc taatacatct gttagccaat ttgcatattc tgtttcccga
    46141 ttgatcgcat gcgctaaaat caggtagtgt ccgtaatttt catgtttttc tttttcagta
    46201 ggaaagatcg ttttttttcg ctctaataga tgaagtcgtt tttcttcatg aaggtcacgc
    46261 tgctgctgaa taatttctac taatgccgga tcattcacgt ctttgacgaa atacaatttc
    46321 agaacaaatt cgtctcgatt aaccggtagt tcattggtgg gtgtatgaat ccactctgcc
    46381 aattgttcct tcccttcttc tgttaattca taaactttct tttccaattt ttcacctgtg
    46441 atctctactt gatgctgaat gattccttgc tcttccattt ttgcaagttc aggataaatc
    46501 tgactatgtt ttgcttgcca aaattctcct atttcatttt tgaacgattg attcaattca
    46561 tatcctgtca tcttcttctt acttaataac cctaacaaaa tatatcgaag tgtatttttt
    46621 cttgccatca gttttccctc cttgacaaat ctttaaatcc attctatcat aaatgagtaa
    46681 tcgaaaacat gtagtttatt acatgtttta atttataagg agatgcttaa acatgaaaga
    46741 atttaaagaa ttggaagatt ttattggaac tcactttatc tatacgtatg acaatggttg
    46801 ggaatatgaa tggtatgcca aaaatgacca tactgttgat taccgtatcc atggcggaat
    46861 ggtcaaaggc cgttgggtaa aagaccaaga agctgatatc gttaaattga cggacggcgt
    46921 attcaaaatc acttggacag agccaacagg gacagacgtg gcattggact ttatgccaaa
    46981 tgaaaagaaa cttcacggaa tgatcttttt ccctaaatgg gttgaagaac atccagaaat
    47041 cactgttact ttccaaaacg aacatatcgc agaaatggaa gcagctcgtg aaaaatatga
    47101 aacttatcca aaattacttg ttcctgaatt tgctacgatc acttatatgg gtgatgcagg
    47161 tatagacaat gaagatgtta ttagcgaaga accatacgat ggaatgccag atgatatccg
    47221 tagcggaaaa tacttcgatg aaaattataa acgagtaaaa taaggttgtg aaagagaggt
    47281 tgtgaaatca gcgttctgac ctgagtatta gagaaagaaa cggaaaaata gttcctcatg
    47341 ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tatcaagagg
    47401 ttgtgaaatc agcgttatgc tttgagtaat aagaacgcta gttagaaaat agcttttcat
    47461 attttctagg ctagtgggct tattaccgag aagcagccgt ttgaacaccg tttatcaaga
    47521 ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaatggaaaa atagttcctc
    47581 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttatcaaa
    47641 gattgtaaca actgcatatt cacacagtta aattagtgac ttcaaaatga aataatgctc
    47701 ccagcaaaaa attaggctgg gagcattatt tcgttattct gttcatcaaa aatcagcgtt
    47761 ttgattccat ttcatcacaa gttcctcctc atttactgct tcatcttttt gctgtgtttg
    47821 aattgtaaaa ccttgtgaaa gatagaaatg aatagctgat tgattttttt gatagactgt
    47881 caaagtcagt gttttattgg tttcttttat ttttttcatt agttgcgtac cgattctttg
    47941 ttgccggtat tcccttttca caaagattcc tgcgataaac tgatcaatca ccccagcaaa
    48001 accaataatt tcttgatcta tttctgctaa gtaaatcact gcatctggta aagctttcct
    48061 aacagattct acttgattct cccaataagt tgatggaatg aattcatgag aatccaagtt
    48121 agcatgcagc caaatcgata gaacagattc cagttctttg gcagacaatt ttgttgttgt
    48181 gcggatcatt ttttctctcc ttacttattc attgatttta aaataaaaac catttttttg
    48241 aagctctttt acaagttttc gcccctcaaa aaagtatatc ttctcttgtt ttacttttct
    48301 aataccaaaa gaaacagcaa ctttaggatc atcagctgaa acgattcgat aagttccacc
    48361 aggcaattga tccagcttga ctttcccatg atggtcgctt tgtactgaaa acggcacacc
    48421 atttctagta agtgtttttt tcttatgttt caaaatgaat gaactaccat ttaccggttt
    48481 ccctgacgaa tcactaataa taaatgaaaa atcaagcact tttttactta atctatactg
    48541 atagattagt cgtccgataa acactaacag tatgactaca gctattatcg ttcctgcaag
    48601 aataaaccat tgccgcatgg ttctttcctg atttcctttt ccttcttctt tttttaccga
    48661 ttcgttataa ggaatccgat gtcctgtcac tagcaaacga tgcgtgttga tcatatacgg
    48721 cgtacatgta agcaaggtaa ctaagtcttc tccttctgaa atcgttagaa cagacgtgtc
    48781 atctggcagt accacttgta tatttttaac ttgataagcc aatttctccc ccaaaacagt
    48841 caaaataaac gtgtctccgt tttctaaacg atctaattcg gtaaataacc gtcgattagg
    48901 taaaccgctg tgagcagaga tcactgaatg cgtcgaattt ccaccaagcg ggaaagaggt
    48961 tccttgcaag acgcctgctc cattttcaag gatcctacca ttgagccgat cataaagtga
    49021 aatatttaat tggatcttag gtatattgat acttccgatc agccattcct ctaattggct
    49081 ggatgttgta ctttcatttc tgttcttttc attgaaagga tcagtaccag gattgaatcc
    49141 ttggtcagtt aatcgttggt ttttctcctc cattttcttt cgctgcactt caatttcagc
    49201 tgcggtccgt ttttgtactt gttcgatacg tttttgatca ataaaactat ttaaagaatc
    49261 cacataaaat ggatataaag cgatcagtaa ccctacgacg atacatatta ataaaaacaa
    49321 acgaaacaaa agtcgacgag actgtttttg cttactctgc ttcacatgtg tcctcccttg
    49381 caagtttttt tgttctttct tttattatat gggttcagga aaaaaataga aaatgaaagc
    49441 cgatgaggtt gtgaaaaagc tgtcctgcat caagtaataa gtacagccca aaaaaatagc
    49501 tactcatatt ttttgttggg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    49561 taaggaagag gttgtgaaaa ctgcgttatg ctttgagtaa taagagcgct agtcagaaaa
    49621 tagcttctca tattttctag gctagtgagc ttattaccga gaagcagctg attgaacacc
    49681 gtttaccgag aggtcatgaa aaagctactt tgtttcgagt attaagaaaa attctgtttc
    49741 aaaacaagga gatgcaacaa aacttatgtc gcatcctctc tccttagaac tttcacttat
    49801 gtttttcttg taaaattcat aaatattttc atgaattttc taatttcttg cctacctctc
    49861 gcataggata tatgatgaat ttatctaaag tttgcaaagg agttcgacaa ttgaaaaaat
    49921 atggcccatt tttagcaatt tttttcattg ctttggcatt tctcatgcct catttattca
    49981 cacataaact aattttgggt gctgatgccc cattccatta caatcggttc tatgatgccg
    50041 cgcaacaaat taaatatggg gatttccatt attttccttc cctttatgga ttccagcaaa
    50101 gtggcaggat cgtgaatgcc gtttacggcc catttttcgc ttatttacaa ggattgatcg
    50161 tgttgcttgc aggtagctgg tttcattatc agattctttc aaatttcatt ctttatctta
    50221 tttcaggatt ttctatgctg attttactga gatatgtcaa tgttcgaaat tgggtaagtg
    50281 taccagtagc cgttttattc atgagtacgt attctattca atactggtct atcaaccaag
    50341 gattcactag ctgggggact tgtttttatc ctttatgtat gatccctatt gctgatttcg
    50401 tccttcataa aaagttccct gttatcaaag tcgctgtctc agtgggactt atgacacaga
    50461 tccatttact gtcttcgatc atgttagctt tgatgtatat ccctttttat ctttattact
    50521 tttttaacca gactgaaaaa ggaaaagcac tgcttcaact gattaagtcg gtcttgcttt
    50581 attttgttct gacagcaaat atttgggcag gattagctat tgtttacagt gggaataaga
    50641 ttcttcctcc ctttgttaac agagaaatga gtcaaaaggc gatagatctg gatggcagtt
    50701 attggctcca ttaccctaag gcctttccta tattgatatt atcaggtttt ttgcttttta
    50761 ttttctttca acaaaagaac accttattcc aaaaaatcgt gttgtacaca agtcttgttt
    50821 tcttttttct ttctactagc ttgattccat ggacgacatt gatcgaaaaa aatgtgcctt
    50881 ttattagttt gatccagttt ccatttcgat ttttcgtgcc atttacgcta ttgcttctac
    50941 tttaccttgc cctttcactg aaccagtggt ctgaacaaaa gtggttccgt cttttaagta
    51001 tatttggcat tcttattttt tcagcacaga cgatccagaa cttataccag catctggaaa
    51061 agtgggaaaa tgaaacattt gtcagtcgac atacttatct attcgatacg ccggaagaag
    51121 caaggaaaac attcttcagc aaagatatgt ctaccagctt gttcgttttc caaaaaacca
    51181 cacctgacta tctgccaatc tataaggaga caaaagacaa taaatacgac cggtataacg
    51241 aagaaatact agaaaacgaa acaactttta taaaaacaca tccaggcggt cttttgacaa
    51301 tcaagtggaa aaacactggg gacaaaactg ttcatatccc tgttatcgtc tacgatcgga
    51361 ctgtgctaca gcaaaatgga aaaacattga ctgattatga agtaacagat atcgggaccc
    51421 caatcgtgaa gcagcaaaaa ggaatcaacg agctcacgct tcattatcaa acgccaatct
    51481 atttctattt cattctttct cttactttga ttggctggtt taccttgcta tgtttattta
    51541 tttatcatag atacaagctt ttacgtgctt aggcaaaagg gataaataca tccgatcaaa
    51601 aaactctcca aacggtataa tcgtttggag agttttattt taatcagttt ttttatgtta
    51661 tatgttatat tttttattcg ttcacatcta attcgatttc caccccgtag tgatcactga
    51721 tgattggact atcgatccca tcaaaaatcc gttgatattt tttaacctgc attccttcag
    51781 ggacgaaaat aaaatctata cgcaattttt ctgtattttg ttcccaacca tctattttct
    51841 tttccacagt agctgctttt cctttttcat tcgcgacaac atacgaatct tgaagaagaa
    51901 ggcaactttc tcttacagta taataacctt gagagtctac tggattattg aaatcaccta
    51961 ggaaaaaaag tggtacgcga gtttccaaaa agtagttttc caagtttttc cactcaaaat
    52021 aaaagccact ttttttatct gtccaccaag aaaagtggca gctcgagaca aaaatgtcct
    52081 gatcatctat ctttgttttc ccaatcaaga tcttgcgtgt ctgataatct tctttctttt
    52141 gaaattggga gacggtaagg acctgacttt ccaatgtttt tttcgtaagc agtgcatttc
    52201 cttcttcata tttatcataa ccgatgtggc tcatttccca actccaaaaa tactcttttc
    52261 cgtgttcctt taaaagctga actaaacggt aggcaaaatt atctttacgg ataggtactt
    52321 gattctttac cggacagaac ttcattagct tcgcaggttc tacttcttca gattccagta
    52381 attggttgat ttcttgtaaa gcaatcactt cataatcttc taacaggatc ttatctgcta
    52441 attcttgcaa ttttttctct ggttctggct caagccagct atgcgtgttg agtgtcatga
    52501 atttcattgg ttttctcctt gtcttcattt cgaaacaaaa gggttgtgac aaaatttttg
    52561 tcacaacctc tccagcaaat agtgttctcc tgctaactcc tcagtaataa gctcaatgga
    52621 ccaggagcta ttttttgttt ggctgttttt atcacttggt gcagggcagc tttttcacga
    52681 cctctccact aaacagtatt caaactgctg cttctcggta ataagcccaa ccaaacgaaa
    52741 aataggagga gctatttttg tttggctgtt cttatgactt gatgcaggac agctttttca
    52801 caacctctcc ttacatttta atggagccga tcactttacc agctgtcact gtacctaatt
    52861 gcttgatcac tacttcttct actttatcgt ttgtaaatac gatcatgatc gttggatctt
    52921 tttcttcgtt ttggatcaca tcaagatcca tctcagctaa tttcgttcct gttcctactt
    52981 cttggccttc tttgacagca atctcaaaag ctggctgatt catttggact gtatcgattc
    53041 ccatatggat caaaacttcg ataccatctt tcgtttccag cccaattgca tgtttgctag
    53101 ggaaaatact tgtcactttt ccagcgatcg gcgcaacaac ttctcttgat gttggaataa
    53161 cagcaaagcc gtcacccatc attttttgtg agaataccgg atcttttact tcagataaag
    53221 gaatcacttc tccagaagct gcggaaacta gttcttgttc tatacctaag tattgcttac
    53281 tacctgcttg tccatttcct gtagaaacca tttctggctc tggaatctct gctcctgatt
    53341 gcagcagatc ttcgatatct gatttcaaga catctgcttt tggtccataa actgcttgga
    53401 ctccattgtc tttgacgatc aatcccatcg cccctgctcg tttccaggct tcttcagtcc
    53461 ctactttttc tcgatcattt acacttacgc gtaaccgagt catacaagca tcgacatctt
    53521 tgatattttc ttttcctccc agcaaatgaa tgatctggac cacttgctga tctgctgttc
    53581 ctgttccgct gttagataca acggtttctt cactgtcatt gtcgtaattt ccgttacgtc
    53641 ccggtgtagc aaagttgaat tttttgatca agaaatttgc taagaaatac gtaacaacac
    53701 caaaagcgat cacacacaac acgaagttga taagatctcc acctagaccc gctttgatag
    53761 ctaacggtgt ccgagtcagc aattcgatat tcccaaatga atggacccgc aatggcaaga
    53821 tatctgccat cgcaaatgca gccccttgga tcactgaata aatgacatat aatggcacag
    53881 cagcaaacat aaacatgaac tccaatggtt ctgttacacc tgttagaaag acagccaacg
    53941 ctgctgaaaa atacattgat ttatatcttg ctttcttatc aggatcgaca ttacgataca
    54001 tcgcaaaagc cattcccatc aagatacctg aagaaccaat catttgtcct actttgaaac
    54061 gagccggcgt ccagttatgc aacacaaact catattgcga catatcccct gcacctttaa
    54121 gattcactaa atcagtggcc caagccaacc ataacggatc ttgcccaaat acttgtgtcc
    54181 ccgcctgtgc gcctgataaa atctcatacg atccgccaag ctgtgtatag ttgataggaa
    54241 tcgtcagcat atgatgcaat ccaaatggca atagtaaacg ttctaatgtt ccaaataaaa
    54301 acggtgctag aatcggtgcg ctttcttgtg attctgcaat ccataaacca aaattattga
    54361 ttccagcctg tacgtttggc cacaccacag caagaatcaa tgccacgatt gttgaccata
    54421 agataacaac aaacggaaca aagcgctttc cattgaaaaa tgacaaagca tccggcaatt
    54481 ttctataatt atagtactta ttataagcca ttgccccgac aaatccagcg atgatcccta
    54541 cgaatactcc catattcaaa gctggtgctt ccaatacact tgtaaaaaat cctttaacca
    54601 tgatcttcgt accgaacaga gtgtgggtaa atgcatcttc attcgcaagc atgtcacttg
    54661 tgacaccaaa gatagcccca gtgatccgat tgattaaaat aaatgagatc ccagctgcaa
    54721 atgctccgcc tgctctttct tttgcccaac ttcctcctat tgctaaagca aataaaatat
    54781 gaagattacc aataattgcc caaccaatat tttcaattac tccgcccgtt gtgacaagcg
    54841 gcgtccaatc aggattgatc aagggaattg atttccctat actgatcatc aacccagctg
    54901 ccggcataac tgctaccaca accattaaag cctttccaaa tttttgccaa aactcaaaac
    54961 taaatatctt tttcattccg tttagctcct ttacgcaatc ggtttcgtta aacgtattat
    55021 agcgtgcgtt ttcttttttg taaatgcttt attttttatt tttctgtcta taccattcta
    55081 taaaacctat aatacgtgtg ttcccttcta taattttctc ctaaaaattt atttttttaa
    55141 gcttttttct atgaaatatt ttcttttgtc tattgacttg tgcaatcggt tgcgtttatt
    55201 atgaacgtat aaaacagctc attaggagga gaaaaatgaa acagatcaaa cgactatttg
    55261 ctattgatcc atggaaaatc agcacgacac aattagataa aaaaaatctt cgactgcaag
    55321 aatctttaac aagtattgga aacggttata tggggatgcg cgggaacttt gaagaaactt
    55381 actcagggga ccaccatcaa ggtacttatt tggcgggtgt ctggtatccc gataagacac
    55441 gtgtcggctg gtggaaaaat ggttatccag aatactttgg aaaagtgatc aacgccttga
    55501 actttatcgc gatagatatt tcgatcaacg atcagctgat cgacttggct tctttggaac
    55561 cagaagactt ttactgggaa ttagatatga aaaatggtgt attatctcgt acattcacaa
    55621 tcactacttc tactaataaa gtacgcattt ccttcgaacg ttttctgagc attgtcaaaa
    55681 aagaatcagc ttatatctat ttaactatag aaatgcttga aggtagcgga acagtcaaac
    55741 tcgtatcaaa attggatgga aatgttcaaa acgaagacag caactatgat gaacattttt
    55801 ggaatgagat tgatcgtgga actatagaag aaatcggcta tttgacgact aaaactattc
    55861 caaacaactt tgggattgaa caatttactg ttactgcagc aatgcgccat ttcgttaatg
    55921 gaaaatcagt catccctgac tatcacgaag atactttagc attacatggt tgcttgtctg
    55981 cagaattatc tcaaggtgag acatggtcag tggaaaaaga aattatcgtt cttactagta
    56041 gagatattcc agaagcgcag caagcagaaa aagctgtctc agaattaacg aaccagacaa
    56101 acaattatat ccaagcaaaa gaagagcaaa cagcggcttg ggcagaacga tggaagcttg
    56161 ccgatgtggt catcgaagga gacgaagaag cacaacaagg tattcgattc aatttgttcc
    56221 aactattctc tacttactac ggcgaagatg aacgattgaa tatcggcccc aaaggattca
    56281 caggagaaaa atacggcgga gccacttact gggatacaga agcgtatgct gtccctcttt
    56341 atcttgcttt agccaaacct gaagtaacta agaatctgct gaaatatcgg cataatcaac
    56401 tgccgcaagc aatccacaac gctcagcaac aaggattaaa aggcgcgctt tatcctatgg
    56461 tcaccttcac tggtgtcgaa tgccataacg agtgggaaat cacattcgag gagattcacc
    56521 gaaatggcgc gattgcctat gcaatctaca attatacgaa ttacaccggc gatacggaat
    56581 atttgaaaaa tgaaggactg gaagttttag cagaaatcgc acgcttctgg gcagaccgtg
    56641 tccatttttc aaaacgagca aataaataca tgatccacgg tgtgaccggc ccaaacgaat
    56701 atgagaacaa tatcaataac aattggtata caaatacgat cgcagcttgg gtcttgcgtt
    56761 acacgcgaga aaattattta acttatcaaa aagaagctgc tatcactatc agcgaagaag
    56821 aactagccaa atggaaagat atcgcagaga acatgtactt cccagttgac gaagaattag
    56881 gaatctttgt ccagcatgac acattcttag ataaagatct catgcctgta tccgatattc
    56941 ccacaagcga tcttcctctg aatcagcatt ggtcttggga caaaatcctt agatcttgct
    57001 tcatcaagca ggcagatgta ctacagggga tttatttctt caatgatact tttacaaaag
    57061 aagaaaagca aaaaaacttt gatttctatg aaccaatgac tgtccatgag tcttcccttt
    57121 cgccaagtat ccattcgatt ttagcagcag aattaggaat ggaaagcaaa gctgtagaaa
    57181 tgtaccagcg aacagctcgc ttggatttgg ataattacaa taacgacaca gaagacggct
    57241 tgcacatcac ttccatgact ggtagctggt tgtcgattgt ccaaggattt gctcaaatga
    57301 aaactgccca tgatcagttg agattcgcac catttctgcc aaaaacttgg aaagcttatt
    57361 ctttccatat caattatcgc ggacgtttat tgttcatctc agtcacagat gaacaagtta
    57421 ctttcgagtt attagaaggt tctcctctag aaatttacgt ctatgaacag tcttattctt
    57481 tagcggatcg tctagaaata acgctaagaa aggaggaagc aaatgtttaa aggggtattg
    57541 tttgatttag acggtgtcat taccgatact gctgaattcc attatcgtgc ttggcgaaag
    57601 cttggtcagg aaatcggcat tacgatcgac cacacattca acgaacagct aaaaggcgtc
    57661 agccgagaag actcactctc tttgctttta gcttatggcg gaaaagaaca ctctttttca
    57721 aaggaagaat ttgcagaatt agcgaaaaga aaaaacgatt attatttaga gatgatccag
    57781 acgatcgaac caaaagatgt cttccccggg gtcgtgcctt tactggattc tttaaaagaa
    57841 gaaaaaatca agattgctct tgcttctgca agtaaaaacg ggccattcct acttgaaaaa
    57901 atgggtctta caccttactt tgatgctatt gccgacccgg cagaagcaac aaacggaaaa
    57961 cctgcgccag atatcttcct ccttgcagca aaagctgtcg gactaaaagc agaagaatgt
    58021 atcgggatcg aagacgcgca agccggtatc caagccatcc tctcaagtgg cgctcaacca
    58081 attggcgtgg gacgtgctga agaccttgga gaggagatcc cgcttgtccc tgatacgact
    58141 tttcttacga tcgattactt gaaagaaaag tggcatgacc atggataatc cagagatctc
    58201 cgtcgtacca gttccagaga ctgaaataaa gaaaatcaca ttgaagaacg actacttaga
    58261 agtcgttctt ctcaattacg gtgcccgcct gcaccaaatc ttcgcaccag acaaagaagg
    58321 aaaatcagaa aatgttttat tgtcttacga tcgtttcgaa gatgttctaa cagacaaaag
    58381 cttttttgga gcaactgtcg gacctgtagc cggaaggatc cgggatgctt cttggggtga
    58441 gcatagattg gagaaaaact gcggatcaca ccatatccac ggtggcacga aaggctggtc
    58501 tttccaattt tgggaagtag aagttttcaa aactccatca tcaataggtg tggtctttta
    58561 cttaaaagat gaattttcta cctatccagg accaatcact gctacgatca cttaccgttt
    58621 aacaaaaaac gaattagaga tgacaacctc tgctagtagc cttgttgaaa ccatctgcaa
    58681 tccgaccaac catgcctact tcaacctttc aggaaatgga acaagagaca tctatgaaca
    58741 tcagttaacg gtctttttag atggtgtgct tgaattagat caagaaaaac tgcctactgg
    58801 aaattggaca aaaaaagaaa aactgccaat cgactttaga aaatccccaa cactgcaaga
    58861 gatccttgca tgttatccag atggactaga tgatgtattt cttttgcatc atcctaggct
    58921 ttccaaaact tccttacaat tattcgagaa gcattctggt cgccaaatga ctatagcaac
    58981 ttctaataaa agtatggtat tattttctac gactggattc gaagctgatt tctctgtcaa
    59041 tggaaaacag atgcattcaa actacggatt agcaatcgaa ccgcaagaaa tccctgatat
    59101 cgttcatttc cccaaatttg gatcgatcaa cctccatcct ggtcaagaac ggatcagtca
    59161 gacgatttat cggttctctg cacaataaca agagaaaggt gtttttatca tggctattac
    59221 cgtaaaagat gtggcgaaaa aagccggtgt tgccacctcg accgtctctc gtgtcatcaa
    59281 cgatcatcct agtatttccg agacaacaaa gaagaaagtc cgaaaaatta tggatgaact
    59341 aggttatgtt cctaacatga ctgcccgcaa tctaggaaaa cgcatttcca gtgccatcgg
    59401 cgtaatctta ccccctcttg attcaaaaga gcggatcggt aacccatttt atcttgagat
    59461 catggaagca atcaacgaag aagcgcggaa ttatgatatg actacagcaa tcgctactgc
    59521 taaaaatttt gaattgctgt tagagaacgt ccagcgtatg catttacaaa aacaagtaga
    59581 tggttttatt ctagtgtatt cagatagtca agatactgtc attgattatt tataccaaaa
    59641 ccacatccct ttcactttga tcggacagcc ttatcatcat gagaacgaaa tcgtttatgt
    59701 agataatgat aaccagcttc taggtaaaca agcaacagaa tttttgatcc agaatggaca
    59761 taagaatatc ctgtttgtga caaacacgac tcatgaaagt ttgtatttcg aacgatattt
    59821 cggttatcaa aaagcaatga tgatggctgg tcttcctgcg ttatccagtg tagtactaga
    59881 gcatccggag gattatctgt cctttgagaa aacgttgaaa gaaaacgaag ccacagcatt
    59941 ggtggtcatt gacgatatct tcgcccttcg cacgatgcag ttggcgcaaa tgtatggcta
    60001 tgtcattcca gagacactct ccgtcattag cttcaacaac tcgatcttct caacattaac
    60061 acacccttac cttacaagta tcgatatcga tatctccgaa ttaggacgtg tcgctatgca
    60121 aaagctgcgc gaattgatcc atgaagaatt gagcagtggc gtacgtttgg tcatccctca
    60181 tcgcttgatc aaaagagaga cagtcgttcc tttagaaaac ccatactaaa tagctgactc
    60241 actaaatacg ctgaccattt atcctatggt cagcttttta tgtaaaagaa gatactactt
    60301 ctaacacatt gtttatctgt aaaaaagagc gtgggacaaa agtaaagaac acttttgtct
    60361 cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa
    60421 attcacaaaa atttaaaaag caattttctt gagtttcttc ttatttcttg gagttaaacg
    60481 cttctgtccc agcctctttt ttccttcctt caatttttta cacgtcgacg attcaataaa
    60541 aaaccgccta atattgtaaa gacaggtgca agaatcgaga aaaaggcgaa tggcaagtaa
    60601 tgcaacgcgc taactttcat cgttcccata atgaatgtgc cgctgactcc ccacggtacg
    60661 agagaattga cggtcgcacc tgaatccgct agtgttcgtg tcaaataggt ttttgataag
    60721 ccaacgtcat caaaagaacg cttgaatgtt tctcctggaa gaatgatgga cagatactgt
    60781 tctccaacga tcagattgat tccgatacag ctcaaggcag taaacccgac taaacgagaa
    60841 ggtcggtcca tcttttcttt taattcttga acgatggtat caacgatcag atatttgatc
    60901 agtaacccac ctaatgccaa tgcaagtaaa atcaaactag cagaacctaa catactcatg
    60961 atcccgcctc gagaaagcag ttcatctagc tttggatcag gtgtatttgc gacataacca
    61021 ttcatcaaga tgtctgctgc ttttccgacc gtcaaacttt tctcatggat aaaagaaaga
    61081 acgagagcag ctgtcgatcc cgctagtaat gtagggatag ccggaatttt tttgattgcc
    61141 cccccaaata aaataactag tggaatcaat gtccaaggag agatccaaaa gctcgctttc
    61201 atgttttgga tcatttcatt gatactagct agatcggctg aatgactgtt atgtcctaat
    61261 atcgtataaa aaatcaacga aaggacgaat gctgggaaat ccgtataacg cataccagcg
    61321 atatgttcga atagattaac tcctccaata cccgctgcca agttggtcgt atctgataaa
    61381 ggcgagcagt tattccctaa aaaggcaccg gaaacgactg cgcctgtagt cattgcatca
    61441 ttgaatccaa gtaaatgacc gatccccaaa aaagcaattc caatcgtcga aatcgttgta
    61501 aatgaactgc cgacagtcac tccgacaatt gcacaaacaa gaaatgctgt cggtaaaaag
    61561 aaacgcacag aaacaagttt aaatccataa accataatcg tagggatggt tccagataaa
    61621 atccatgaag caactaagac accaatcaaa agaaaaatca ggattggaat gatccctgga
    61681 gtgattcctt tgatgatccc gtcatggatc tcatcccatg tgaacttttt aaatctgcca
    61741 taaaacaaca aacacatgaa agctgcaagg atcgggatat gtggcgagag ctggaatccg
    61801 atgatcagta ctcctaggag tgccaacaac acgatcaata cagcgatact ttcttttaaa
    61861 gaaatatttt tattcatcgt cgatctccta tttcaaatga aaaggccctg ccatgaaaaa
    61921 actattcact tggcaggacc tcctactaaa tcagcgacaa aacgtcggct gaaagactta
    61981 tttttttatt tttcttctaa acttttttct acctcaatcc cccaagaatc tttcttgaag
    62041 tgattgatga tcaatggaat aacgaacatg accactgtga tagctgctac taataatgca
    62101 tcactcaatg tcgctgtacc taatgtagca aatacgacaa cgatgataga gaagatcagc
    62161 atgcagctga ctacccaagc taatccgttt ccgtttttac caattcggta aggacgttcg
    62221 aattctggat gtttcttacg cattttaagc aatgaaacag caatcaaaat gtacacgata
    62281 caataaataa cagttgtagc atttgtaagt gtcaagaata cagcacttac gtcatccatt
    62341 acaccataaa gcaaagcaaa taatgaaata acgattgatt gtgttagaac gacatttcta
    62401 gatacgccgt atttgttttc tttatggaat ccgaattttg gtggaaggaa tccttcacga
    62461 gctacttgga tgattgtttt tgatggtcca gtaacccaag ctgataattg taaaagcaca
    62521 ccaataaata ccatgaagct gaagatattt ccgatcactt ctggtaagcc taggatttga
    62581 cagtcaatca agataggctg cgtgatattt gctaattcca tttttccatc tggtacgaca
    62641 tttgctacaa gcatcgcatt gatgacattc aataaaacta atccgatcaa agcgataaat
    62701 acgccttttg tataattttt tgttgcgtct ttcaaacgag gcatgtaaac agaactcatt
    62761 tcgatcccaa caaaaataaa tgtgatacca gctaagtatt tcaatgaatc aatatgttcc
    62821 aagtcaggga aagcttttga ccatgaaaag tcacctagat agccgtttga gactagtccg
    62881 actttgaagg ctgccaaaac acctaaaacg aacatgatga ctactgggat atatacccct
    62941 agccatacac cgatgttccc gccgactttt gccatatcga atttcaagtt caagatcgtg
    63001 atgatccagt agataactaa aatacaacct aaaacgaacc agtgattatt gccaagttct
    63061 acattaccaa atgtgtttcc taacaatggt cctaaagtag aagcgaccat taccatacct
    63121 gggaacattt gcacccataa tagccatgca acgacaaatc cccatttgct gcccagtgct
    63181 gtttttaccc ataactgagg accgccttct tgaggaagca tcgtggaaag ctcaccggaa
    63241 accattgata tcggaccggc aaaaaacaaa actgcaaaaa ttgaataaaa aatcaatgtc
    63301 caaccgactg cggctaacgt aggaatactc cgaaccgttg cacacagtgc cattgtcaga
    63361 ccgataaagg tcccaagacc cattttactt acttcagatt tactcaccgt tgacactctc
    63421 cttgattttt tggataaaat aacggtgtct ttgaagtagt ttcagcaata aacgaaacaa
    63481 ttcagcgaat atttgagggg attctttgag tttattgctt gaagggtttc ttcaaagaca
    63541 acctcaaaac aaagggatcg aagagcttag gtctcccctt gctcttcaga tccaaacatg
    63601 cgtcagaaac agttagtatt caagctgttt tctaacatga ttaccttgct tattttgctt
    63661 cgcttgccaa taattggtct gcgtagattt tttccaattt ttcttgaaga gctgctttga
    63721 ttttttcagc atattcttca aagtgttctt ctttgttcat gtatggtgtc attacgctgg
    63781 cacgcaatac agttactttt ccggcacggt tccattcttc atctgagaat cctagttggt
    63841 tcacaaattg taatgggctg ttgccatagt ctgggatagc aaagtcagta tgtgaagtta
    63901 agaactcatt accgtaaatg cttcctttca cgtatgaaga ataatcatat acgtcatggt
    63961 tcaatttatt cattgcaact aagtcatcgt tgcctttttc tttgaataca tagtctacca
    64021 tattgaaatc tggataagtt aatggatgaa cttcgatttc tttgtctcct actttaaatg
    64081 acaagtcatt caagaagttg tagaaacgat gtgcaccttc gattgatgca cccattaatt
    64141 taccatatcc tgtgacattc aaaggtaaga catggtgtgc tgcccaaacg ctggctgctg
    64201 ttgcccctgc ttttgaacct tccaagatat aagccccaag taatgctggg atatctgcgc
    64261 ctttttcaaa tacatatgtc gcaaagtaag agataacgtc tctcatgcgg atatctttga
    64321 tgacgatacc accggcagaa tatggaacat agcccatttt atgtgggtca atcgttacag
    64381 attctgcttc ttcgattgct ttatatgcac tgtgtacttc ttctaagata taatctttgt
    64441 tttctgtaaa gacattgtat ttgtaatgaa catcttttaa atcttcaaat ggaatgaagt
    64501 tgttgtcttc gtctaagaaa atcgcacggc cgtatccgcc ataagcagca tctacgtgta
    64561 agtagaagta aataccgtct ttttctaata cgcgacgcaa tgcaacgatt ttatcgatac
    64621 cgtcgattgc tccttcttct gttgatccta caacaccgac tacacctagg attggtgttt
    64681 tttcagctgc tagaccacga acgatttttt caagttcgtt gatgtccatg cgatagttgt
    64741 ggtcaactgg cactggaatc acttggtcta aaccgatacc gatgatgtcg gcagctttta
    64801 gccatgaata gtgtttagtt tgtggaacca accattttcc taatttttct aaatgttttc
    64861 cgctacgtgc tgagtgagct ttgatttcgt cgattttttc tgggacgctg tctaacagat
    64921 tcatgatttc ttctgttgac aagttcatta attcccaatc gctttttcca gcaactagtt
    64981 caggtgtcac ttctttcatt gcaagcggca atgatttgat gttgcgggca taccaaagtc
    65041 cttctaagtt tgctaaagaa ccatcagcaa cgatgtggcc ccagccatct ttatagctca
    65101 ttaattttgc aaattccatt cctacttctt cttccatttg gcttgttgca ggagaagatt
    65161 cataggcaac gttattacca ttccatagca tagcaaaatt gtacgctagt aaagatggca
    65221 tcaatgtttc agagttcata tgaccccagt aacgtccggc attatgccaa ggaacggaat
    65281 gggtacgcat ccgcgctgaa atttctgata gaacatcctt tgttttattc acagtgtgtt
    65341 caaaactagc actgcttttt tcttctggcg taataatagg catgtcttga ggcatgtagt
    65401 tttgacgcca gcctaaatgt tcatccacca attcattcaa caaagcttta tagatttggc
    65461 cgttctcagc tttgtcccca atgaataggg catttaggtt tagatctttc gacaatgatt
    65521 cactcatagt gaaaccctcc taaaaaaata tttaaatttt gtcgagccct acattacacc
    65581 tgttcttcta ttctgtaaac taagttttgg cctgattttt aatcatattt taatatgtat
    65641 caattatccg aattgttcac acttttgaaa taattaaaaa aaacgaggga atttacaaat
    65701 gtaaactccc tcgcaaagat tgtattaacg acaaccagcg ttctatttta ggatgggttt
    65761 caaaattgga atgttgtaat agtatttccc tttttcaagg taaaaactta ctaaattcta
    65821 atcaattact ttggccaaaa agtaattttt cttacctttt cgaataacca caaacttacc
    65881 atcgaattca tcggatggat ttatgacaaa atttaaatcg gtaacgcggt caccgttaat
    65941 acttatagct ccgtttgaaa catcttcacg tgcttgacgt ttagatggtt caatttttgt
    66001 ggaaacaagc agttccacga tattttcagg tgtgcttgaa atttcaacgt tcggcatttt
    66061 accgaatcct tgcgcgattt cttccgcatt tagttcttta atatttccag agaacaatgc
    66121 ttgcgttatt ttttgcgctt ctttcaagtc ttcttcacta tgcacaaatc ttgtaacttc
    66181 ttctgccaaa cggcgttgtg cttcacgttt ctcaggttct gtctctactt ttttcgctaa
    66241 gtcttcgatt tcttcttgag acaagaaagt aaagaatttc aagtatttca ctacatcgcg
    66301 atcatcttga ttcaaccaga attggtagaa ctcaaatggt gaagttttct tcggatcaag
    66361 ccaaacggca ccgcctgcag tttttccgaa tttcgttccg tcagccttca gcatcaatgg
    66421 gatcgttaaa ccgaacgctt ctgcttctgg tccttctaat tttcggatta ggtctaatcc
    66481 agctgtgata tttccccatt ggtctgctcc accgatttgc aattgcacat cgtatgtttt
    66541 atgcagatgc aaaaagtcga tagattgcag gatttggtaa gtaaactctg taaatgagat
    66601 tcctacttcc aaacggctgg caacgatatc ttttgctaac atcgtgttga tgttgaagtt
    66661 tttaccgtag tctctcaaaa attcaagtaa agagattttt gacaaccagt cgtagttgtt
    66721 cacaaatgtg atatttgcat ctttaccaaa caattttctc atttgattgg aaagtgcgtc
    66781 cacgttatgt tgcacttgtt ccatcgtttg caacacacgt tctgttttgc ggccgcttgg
    66841 gtcgccgatc gttcctgttc cgccgccaat cagaatatat ggatgatggc ctgccaattg
    66901 gaatcttttc atcatcataa atggaatcaa atgtccgata tgcatcgaat caccagttgg
    66961 atcaacgccg caataaagtg aaatgctctt ttcttcaacg agctcgcgaa gtctttcttc
    67021 gtttgtttgc tgattaatgg cgtcgcgcca agttaattca tcaataatat tcattttact
    67081 gacctccgtg tagttttttt atgaaagcag gaacctgcta gcatatgtaa ttaattattt
    67141 cttcaggata ggataggaaa taaaaaaagt cccttggcat ataagccaag ggacgaaatc
    67201 tcgcggtacc acccaatttg tatgtctaga tatccaacat acctctcgaa catttaacgc
    67261 tgtcgcgtgg caatcactgc caaagactcc tgaaatgtaa ttcgctttaa agcatgtact
    67321 ggtttgcagc gaccaccagc tttctgaaac attggcttta acaactactt cgtttcgatc
    67381 ttcgtcttgt tattattaat atgtgtctat catagagcgc aactttttct ttgtcaacag
    67441 aaaaaggata ttttttttaa attcctcatt ttttcttttt tgaatccgct ctaattccga
    67501 acattttatt ttcaattatt gttaaaagaa ataacttttt ctgtattttt tctgcaattt
    67561 atcgagaagt cactttactt tgcctcttat tctttcttcg ttacaataag gaatgggaca
    67621 agtaaaaaaa tgaaaaagca agggacgagg tatatggaaa aaaagcaaaa aattatttta
    67681 ggagccactt ctgctttaac tatcgttatt ttaattctaa gtttgttttt tgtcatccga
    67741 tcatctacaa aaaaagaacc aatcattcct gaaaaaaaaa taacagaaaa agcaaaagaa
    67801 ccgacagatg ataagcaaca actagttact caagcaaaaa atctggctca aagctacgat
    67861 tatgaccaag cgattgcgtt attggaaaaa gatagtaaga acggatctac tgaaacacaa
    67921 cagcttcttc aaacactaaa aaaggagaaa gaacaactga tcgactggtc tgatcctact
    67981 cagatctctc atgtcttttt ccacagcttg atcgtggacc cgcaaaaagc cttccactct
    68041 caacaggcac aaggatacaa agattatatg gtgactgttg aagaattcaa ccgttcgatc
    68101 gaacaacttt atcaaaatga ttttgtttta gttaatttga atgatttgat ccaaaaagac
    68161 gaacaaggaa atctttcctt taacgggctt gcgctacctg aaggaaaaaa accattggtt
    68221 ctttcacaag atgatgtcag ctattatgaa tatatggata attccggttt tcctagccga
    68281 ttggtcgtat caaataacaa tgaaatcaaa aatgtctata aagagaaaga taaagaagaa
    68341 atcggtgact ttgacatggt tcctctgatc gatgcctttg tcaagaaaca tcctgatttt
    68401 tcttatcgag gcgcaaaagg taccttggcc ctgacaggtt acaacggagt tttaggttat
    68461 cgaacatcaa aatctcaata tggagacaat gaaaaaacaa aaaaagagat taaagaagct
    68521 aaaaaagtgg cggaacagtt gaagaaagac ggctggagct ttgcttccca tacatggggt
    68581 cacttgaata tgactcaagc ttcactagaa gatgtaaaaa aagacaatga attgtggcag
    68641 caagaagttg cacctatcct aggaaaaaca aatattctga tttatccatt tggtgcagac
    68701 atcagcgact ggcagcctta ttcagcagaa aatcaaaaat ttgcttactt gaagagtcaa
    68761 ggtttcgata ttttctgtaa tgtcgatgct tctacaccag cttggggaca attaggtacg
    68821 gatttctatc gaaatgctcg gatcaatatc gatggcattc gttttcaatc tgatttagac
    68881 ggaaaaaatc ctattcttga tccattcatc aatgtccatg atgtctatga caaagaagca
    68941 cgcgaatctg cttgatttta cgtcatttat gaaccaaaaa agccgaactg acattttatc
    69001 gtcagtttcg gcttttttat gctttatttc ttcttcggtg cgattccctt gatcaggact
    69061 tctgtccatt ctgctgtata tggaagcagt tttgcgtata tctccggctt agaaattcct
    69121 tctaccattg cttgaaaata gaggatcaag aactcatccg aatatttcaa atccactttt
    69181 ccttctttcc gccctcgatg gaaaagatca agcatgatac tgaaactttc ttgcgtatac
    69241 gcatccatcg cgtcagaaaa ttcttttttt tctttttttg tatagtatgt catcaaatcg
    69301 atataaaatt gtttgttcac tcgattgaga taacggattt tgttttcact catggcaatc
    69361 agtgtttctt caaatggaag attttccgca atcaacgctt tcgcttcttg ccctaaatca
    69421 ttcataaatg caaaaaatac ttcataaatc agcccttcct tgctgccaaa atatttaaaa
    69481 atcgtcgctt tacttacctg agcgttctca gctaccttat ccattgtcac tccagcggtt
    69541 cctatgtctg tgttcatcaa gtcaaaagct gcttgtaaaa tctctttctt tttctcttgt
    69601 gctctctttt caaaaccgtt catttgtaga gcctcccgta attttaaact tttataatat
    69661 atatagttca aatttattct gaggtttatt ataactatct attgagaaag tatcaagcta
    69721 gcaattcctt gtttatgaat tcattgacag aaatttataa aaagaatatt a
//
LOCUS       DASNNU010000004        61088 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.004, whole genome shotgun sequence.
ACCESSION   DASNNU010000004 DASNNU010000000
VERSION     DASNNU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.004"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(31..1422)
                     /gene="sufB"
                     /locus_tag="U8116_000229"
     CDS             complement(31..1422)
                     /gene="sufB"
                     /locus_tag="U8116_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEN8433253.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(1427..1897)
                     /locus_tag="U8116_000230"
     CDS             complement(1427..1897)
                     /locus_tag="U8116_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEN8433254.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(1884..3119)
                     /locus_tag="U8116_000231"
     CDS             complement(1884..3119)
                     /locus_tag="U8116_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN8433255.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(3116..4402)
                     /gene="sufD"
                     /locus_tag="U8116_000232"
     CDS             complement(3116..4402)
                     /gene="sufD"
                     /locus_tag="U8116_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEN8433256.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(4415..5188)
                     /gene="sufC"
                     /locus_tag="U8116_000233"
     CDS             complement(4415..5188)
                     /gene="sufC"
                     /locus_tag="U8116_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEN8433257.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(5385..6233)
                     /locus_tag="U8116_000234"
     CDS             complement(5385..6233)
                     /locus_tag="U8116_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN8433258.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(6257..6943)
                     /locus_tag="U8116_000235"
     CDS             complement(6257..6943)
                     /locus_tag="U8116_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433259.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(6936..7970)
                     /locus_tag="U8116_000236"
     CDS             complement(6936..7970)
                     /locus_tag="U8116_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN8433260.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(8033..8289)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8365..8703)
                     /locus_tag="U8116_000237"
     CDS             complement(8365..8703)
                     /locus_tag="U8116_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEN8433261.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(8705..9061)
                     /locus_tag="U8116_000238"
     CDS             complement(8705..9061)
                     /locus_tag="U8116_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEN8433262.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(9067..10254)
                     /locus_tag="U8116_000239"
     CDS             complement(9067..10254)
                     /locus_tag="U8116_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN8433263.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(10445..11290)
                     /locus_tag="U8116_000240"
     CDS             complement(10445..11290)
                     /locus_tag="U8116_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN8433264.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            11524..12489
                     /locus_tag="U8116_000241"
     CDS             11524..12489
                     /locus_tag="U8116_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEN8433265.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            12866..13909
                     /locus_tag="U8116_000242"
     CDS             12866..13909
                     /locus_tag="U8116_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEN8433266.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(13971..14435)
                     /locus_tag="U8116_000243"
     CDS             complement(13971..14435)
                     /locus_tag="U8116_000243"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEN8433267.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            14681..15628
                     /locus_tag="U8116_000244"
     CDS             14681..15628
                     /locus_tag="U8116_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN8433268.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(15839..17911)
                     /locus_tag="U8116_000245"
     CDS             complement(15839..17911)
                     /locus_tag="U8116_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN8433269.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(17844..18221)
                     /locus_tag="U8116_000246"
     CDS             complement(17844..18221)
                     /locus_tag="U8116_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEN8433270.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(18350..18928)
                     /locus_tag="U8116_000247"
     CDS             complement(18350..18928)
                     /locus_tag="U8116_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN8433271.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            19060..20352
                     /locus_tag="U8116_000248"
     CDS             19060..20352
                     /locus_tag="U8116_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN8433272.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(20412..21530)
                     /locus_tag="U8116_000249"
     CDS             complement(20412..21530)
                     /locus_tag="U8116_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEN8433273.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(21544..22965)
                     /gene="arcD"
                     /locus_tag="U8116_000250"
     CDS             complement(21544..22965)
                     /gene="arcD"
                     /locus_tag="U8116_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEN8433274.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(23211..24332)
                     /gene="mnmA"
                     /locus_tag="U8116_000251"
     CDS             complement(23211..24332)
                     /gene="mnmA"
                     /locus_tag="U8116_000251"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEN8433275.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            24736..25335
                     /locus_tag="U8116_000252"
     CDS             24736..25335
                     /locus_tag="U8116_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEN8433276.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(25473..26759)
                     /gene="efmA"
                     /locus_tag="U8116_000253"
     CDS             complement(25473..26759)
                     /gene="efmA"
                     /locus_tag="U8116_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEN8433277.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(27030..27374)
                     /locus_tag="U8116_000254"
     CDS             complement(27030..27374)
                     /locus_tag="U8116_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEN8433278.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(27511..28656)
                     /locus_tag="U8116_000255"
     CDS             complement(27511..28656)
                     /locus_tag="U8116_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN8433279.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(28836..29810)
                     /locus_tag="U8116_000256"
     CDS             complement(28836..29810)
                     /locus_tag="U8116_000256"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN8433280.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(29890..30765)
                     /locus_tag="U8116_000257"
     CDS             complement(29890..30765)
                     /locus_tag="U8116_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEN8433281.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            30895..32802
                     /locus_tag="U8116_000258"
     CDS             30895..32802
                     /locus_tag="U8116_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEN8433282.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(33343..36621)
                     /locus_tag="U8116_000259"
     CDS             complement(33343..36621)
                     /locus_tag="U8116_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEN8433283.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(36892..37437)
                     /locus_tag="U8116_000260"
     CDS             complement(36892..37437)
                     /locus_tag="U8116_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN8433284.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(37449..38141)
                     /locus_tag="U8116_000261"
     CDS             complement(37449..38141)
                     /locus_tag="U8116_000261"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEN8433285.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(38160..38444)
                     /locus_tag="U8116_000262"
     CDS             complement(38160..38444)
                     /locus_tag="U8116_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433286.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVFFLAIAFI"
     gene            complement(38457..39020)
                     /locus_tag="U8116_000263"
     CDS             complement(38457..39020)
                     /locus_tag="U8116_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN8433287.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            39248..39910
                     /locus_tag="U8116_000264"
     CDS             39248..39910
                     /locus_tag="U8116_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEN8433288.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            40034..41230
                     /locus_tag="U8116_000265"
     CDS             40034..41230
                     /locus_tag="U8116_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEN8433289.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(41331..42707)
                     /locus_tag="U8116_000266"
     CDS             complement(41331..42707)
                     /locus_tag="U8116_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN8433290.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(42744..43610)
                     /locus_tag="U8116_000267"
     CDS             complement(42744..43610)
                     /locus_tag="U8116_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEN8433291.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(43699..44724)
                     /locus_tag="U8116_000268"
     CDS             complement(43699..44724)
                     /locus_tag="U8116_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN8433292.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(44742..45416)
                     /locus_tag="U8116_000269"
     CDS             complement(44742..45416)
                     /locus_tag="U8116_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN8433293.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(45600..46493)
                     /locus_tag="U8116_000270"
     CDS             complement(45600..46493)
                     /locus_tag="U8116_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN8433294.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(46567..48264)
                     /locus_tag="U8116_000271"
     CDS             complement(46567..48264)
                     /locus_tag="U8116_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN8433295.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(48257..49570)
                     /locus_tag="U8116_000272"
     CDS             complement(48257..49570)
                     /locus_tag="U8116_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN8433296.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(49570..53607)
                     /locus_tag="U8116_000273"
     CDS             complement(49570..53607)
                     /locus_tag="U8116_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN8433297.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(53620..54078)
                     /locus_tag="U8116_000274"
     CDS             complement(53620..54078)
                     /locus_tag="U8116_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN8433298.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(54376..54894)
                     /locus_tag="U8116_000275"
     CDS             complement(54376..54894)
                     /locus_tag="U8116_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN8433299.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(54896..55512)
                     /locus_tag="U8116_000276"
                     /pseudo
     CDS             complement(54896..55512)
                     /locus_tag="U8116_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(55509..56372)
                     /locus_tag="U8116_000277"
     CDS             complement(55509..56372)
                     /locus_tag="U8116_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN8433300.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(56359..57270)
                     /locus_tag="U8116_000278"
     CDS             complement(56359..57270)
                     /locus_tag="U8116_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN8433301.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(57311..58645)
                     /locus_tag="U8116_000279"
     CDS             complement(57311..58645)
                     /locus_tag="U8116_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN8433302.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            58853..59683
                     /locus_tag="U8116_000280"
     CDS             58853..59683
                     /locus_tag="U8116_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEN8433303.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(59927..60245)
                     /locus_tag="U8116_000281"
                     /pseudo
     CDS             complement(59927..60245)
                     /locus_tag="U8116_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(60367..60561)
                     /locus_tag="U8116_000282"
     CDS             complement(60367..60561)
                     /locus_tag="U8116_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433304.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT        17241 a        13150 c        11000 g        19697 t
ORIGIN      
        1 atatcaaggg ttctagctcc tataacaggt ttacccaaca ctgccttcca tctcataact
       61 gatcaatcga ttgagttcga ctgcatattc cattggaagt tctttggtga atggttcaac
      121 aaagcccatg acgatcattt cagttgcttc agattcggtc aggcctcggc tcatcagata
      181 gtagagttgt tcttctgaga tttttgagac tttggcttcg tgttctaaag agacttggct
      241 gttgtggatc tcattgaatg ggatcgtatc ggatttcgac cattcgtcca tgatgatcgt
      301 gtcacattcg atatgagaga tcgaacccgc actgtttttg gcaaaggtta cttgtcctcg
      361 atagtttact tctccgccgt cttttgaaat tgatttagag acgatagagc tagatgtatt
      421 tggtgcattg tggatcattt ttgcacccgt atcttggatt tggtttgctc ctgcaaaggc
      481 gatggagagc atggtgcctc gtgcaccttt tccatctaag taaacacttg ggtatttcat
      541 cgttgttttt gcacctaggt ttccgtcgat ccattcaacg gttgcgcctt cgtatgcttt
      601 tgctcgtttg gttactaagt tgtacacatt gtctgaccag ttttggatcg ttgtgtaacg
      661 gcaatacgca tcttttcttg taaagatttc tacgatggcg gcatgcaggc tgttacttga
      721 ataagtaggt gccgtacaac cttcgacata gtggatgctt gctccttcgt caacaatgat
      781 cagcgtacgt tcgaattgcc ccgtgttttc cgcattgatc cggaagtacg tttgtaatgg
      841 aacatcgact ttgacgcctt ttggcacgta aataaaggtc ccacctgacc atacggctga
      901 attcagcgct gctaatttgt tgtcggttgg cggtacaagt tttgaaaagt actctttgaa
      961 taattcaggg tattctttca aagcagagtc agtgtcagta aagacgattc ctaacttttc
     1021 gaattcatct ttcatattat ggtacacgac ttctgattca tattgtgcgg aagcaccggc
     1081 tagataagct cgttcagctt ctggtatacc aatcttttca aatgtatctt tgattttttc
     1141 tggtacgtct tcccagtcac gagcgggttt gtcacttggt ttttggtaat atttgatggc
     1201 atcgaaatcg atgtctgaaa ggtcggggcc ccatgtttgc attggcattt tgttaaatgt
     1261 ttctagagat ttcagacgga attcaagcat ccattctggt tcgcctttga ttcttgacat
     1321 ctcacggatc acttcttctg tcaagccttc tcctgtactg aaaaccggtt cgacatcatc
     1381 gtgaaacccg aatttatatt cttctaattc tggtacgccc atgttctcac tcctcttcct
     1441 tgtggtaatg atggaaagct gcggtaccat cgttttccat tgcttttcct agtgctttcc
     1501 aggcaagtgt cgcacatttg atccgagcgg gaaatttagc tacaccacca agcatagcag
     1561 catcgcctaa ttggtcggga tctttgactt cttttccttg gacaagctgt agaaagtcta
     1621 cagttaaatg ttctgcttct tctattgatt ttccgatgac ggcgtctgtc atcatcgagg
     1681 cacttgccgt actgatggag cagccactgc cgtcaaaggc gatatttttg atgattccgt
     1741 cttttacatc gacttgcaat tcgatcacat ctccacatgt ggggttgttc aattcgatct
     1801 tgcttgtcga gtcagtcaag cttccacgat gatgcggatg ggaagaatga tccaagatca
     1861 cttgacggta taaattgtct aatttagata gtcccatgtt ggaaaaactc ctttgttgcc
     1921 atgattgctt caactaaacg gtcggcgtct tgcgttgtgt tgtagaaata aaagctcgcg
     1981 cgagcagtcg ctgatacttc aaggtacttc aatagcggtt gcgcacaatg atgacctgct
     2041 ctgacggcta ccccttccat gtctaaagct gtcgcgacat catggggatg taacccatcc
     2101 aaattgaatg caacgactcc tgtccgaaaa ttcgggtctt gtggaccata aacagtcaat
     2161 ccatcaattg cttgtaattt tggcaacaca taagcggcta gttcttcttc atatcgatgg
     2221 atattgtcca taccgagttt ttgcaaataa tcgattgcat ggcctaatgc gatagcgccg
     2281 gcgatatttg gtgtccctgc ttcgaatttc caaggtagtt ctttccatgt actgtcgtat
     2341 aattcgacaa aatcgatcat ttcgccacca aattcaacag gttccatttc ttctaaccat
     2401 tttcgtttac catataaaac accaatacct gttggtccgc acattttgtg tccgctgaaa
     2461 gcaaagaaat cacagtctag tgcttggaca tcaatggcca tgtgcggggc agattgagca
     2521 ccatctacta cgatgacccc gccgttttca tggacaagtt cagctaattc ttgaacagga
     2581 ttgaagacac ctaagacatt ggatacatga gcaatggaga cgattttggt tttgtccgta
     2641 atctttttcg ctgcatcctc catatccagg aagccttctt ctgttagttc gatatatttc
     2701 aagacagcac ctgttcgttt tgccaactgc tgccacggga taacattaga atgatgttcc
     2761 atgtaagaga gcactatctc gtctcctgct ttgatatagc ggtcgccgaa ggttctggct
     2821 acccaattca agcttgtcgt cgtaccacga gtaaacagca cttctgctgt ttcttttgca
     2881 tggatgaacg cccgtacttt ttctcttgct tgttcataat catgggtcgc gcgttctgct
     2941 aacgtatgga caccgcgatg aacattggcg ttatcgtgtt cgtaatagta cgctaatgcc
     3001 tggagtactt gttttggctt ttgggtcgtc gctgcattat ccagataaac taaaggttca
     3061 tcattgacgt cttgaaataa gataggaaaa tcattgcgta gctttttagc atcgatcatc
     3121 cattcaactt cccttcgatt acttcgacta attctttccg aacttgttct acagggattg
     3181 ctgttaagac agaaccgagg aatcctcgaa tcactaagcg ctctgcttct tctttatgca
     3241 atcctctact catcagatag tacatttctt ctggatcaac tcgtcctaca ctggcagcat
     3301 gtccagcagt tacttcgttt tcatcgatca aaagaatagg attcgcatca ccacgtgctt
     3361 tatcagaaag catcaagaca cgactttctt gttgggcatc tgcacctttt gcccctttca
     3421 agatgtggcc gataccatta aaggtcaatg ttcctttttc tcggatcact ccgtgttgca
     3481 aaatatgccc gatcgtatga ggggctttat tggttactcg tgtatcgatt ccttggattt
     3541 gtctgcccga gctgattgcc acgattttga cttctgcgtg tgcaccttct cctgctaaat
     3601 ctgagtcaaa gtcagcgatc acatgaccgt cattcatgac accaattgcc cagtcgacag
     3661 aagcatctcg taaaatgtgc ccacggcgat tcatataaga agtaatattt tctcctaatt
     3721 gatctacggc tgaatatttg atttttgaac cagctttagc gatgacttcc acgatgatat
     3781 tacctgatga ttttgctact tgttctttgg ttgtctggaa acgttccaga taagaaaatt
     3841 cactatgttc atctgccacg atcaacacat gcttgaagaa gtgctcatcg gatgcgccat
     3901 cttgaatgaa taaggactcg agtggttcat cgatcacgac atttttaggc acatacagga
     3961 aaataccgct gttcataaaa gctacatgga gtgcagtcaa ttggtcttcg ttcatgttta
     4021 ctgctttttt catataatat tcttggacta gttctggata ttcgatcatt gcagtgaaca
     4081 agtcggtaaa aatgactcct ttttctgcta attccacagg cagttgttca aaaatcgtcc
     4141 atgaaccttg ttggacaagc acaggattgt ctttcattgc atcaaatgcc ggaatcgtgc
     4201 ctgtttcact gatggtttgt gtttctttca catcaaataa cgaccagcga tggaatttca
     4261 cccgatcgat atgcggcaat ggcagttcat ctgctttcgc tagtgcgtct tgacggaagg
     4321 ttctcatcca atcaggttcg cctttactta aagaaaaatc atttaccgcg tcaagataat
     4381 cgagccagtt tttttctttc attcttgctc ccccttacgc ttcttcttct ttatagtcga
     4441 ttcctaattc tttactgata ccagcgtagc cttctgcttc cagacgttta gctaggtcag
     4501 cattccctgt catgactact cggccttcca tcatgatgtg aacgacatca ggtgtgatat
     4561 agttcaataa tcgttgatag tgggtgatga tcaatgcacc gaaattgtct ccgcgcattt
     4621 catttactcc tttagcaacg actttcaaag catcgatatc cagacctgaa tcgatttcgt
     4681 ctaaaattgc aaatgtaggt tctaacatca acaattgcag gatctcattc cgtttctttt
     4741 ctccgccaga aaaaccttcg ttcagataac gttcagccat ttcttctggc atatttagta
     4801 aagccatttt ttcatctaac tttttcaaaa atgccataac agaaattttg tcgttttcat
     4861 cacgtttcgc attgatcgct gcacgcatga attccgcatt cgtgatacct ggtatctcac
     4921 ttggatattg catagctaaa aataagccaa gtcgcgcacg ttcatctact tccaagtcta
     4981 aaacattttt cccgtcaaac aaaatttcgc cttctgttac ttcatagttt ggattgccca
     5041 tgatagcggc agataaggta gattttcctg tgccgttagg tcccatgatg gcatggattt
     5101 ctccggtttt cattgttagg tttacgcctt taaggatttc tttttcctcg atcgacacat
     5161 gtagattttt tatttctaag acagacatgc attatacccc caaattctct ttcgttctca
     5221 ctcattttag actaattgag aatgaagcgc aatgaccttc cttactaaaa tcaaatatct
     5281 tttatttatt attaaacaat tttgatttct aaattagaat aggtataaaa aagatgagaa
     5341 ataagccatt acagacttat ttctcatctt ttttgttcca taccttattt tgcatcagcc
     5401 tttactggtt taacagagcc gccccatttg tctgtgatcc attcttggac ttctttgcgg
     5461 tgaagcactt caatcagttt tttgattttg tcactgtctt catcaccttt acgagtcgca
     5521 ataatattca cgtaaggaga gttgtccttt tcaagcaata cggcatcttc taatggattc
     5581 aaccctgcgc cgtaagcgaa gtttgcgttg attgcaacta ggtcaccttc atcattttgg
     5641 tacgtagaag ctaaaagtga tgggtcgatc gtatggttga acttcaagtt cttagggttt
     5701 tcttcaatat catcgaatgt tgctgtttcc agatctactc catctttgac tttgactaag
     5761 ccagcatctt gtagaattgt gatgatgcgt ccccagtctg attcagaatt tgaagtaatg
     5821 attgttgcac catcttttaa ttcggaaaca tctttgactt ttttagagta caagcccatt
     5881 ggttcgatgt ggacagcacc ggcattgaca aagtcatatc ctttttcttt gatttctttg
     5941 ttcaaataag gaatatgctg gaagtaattg gcatcaatgt cgccttcttc caaagattgg
     6001 ttcggtaata catagtcatc gaatttgacg atttcaagat cgattccttc atctttcaat
     6061 aatggtttca catgttccaa aatctctgcg tgcggtgtcg gtgaagcgcc gactttcaat
     6121 gtcgtgtcgt ctgctttatt tgctgaatct ttgctgtcgc tgttgttacc gcatgctgct
     6181 aatcctacag tcagcaaaag cgctgctgca aatccaaaaa ttttcttttt catatcctac
     6241 atccccctat atcctattat cttttgtctg ttttcttagt taagaaatca ccgattcctt
     6301 gaacgataaa tacgattagt aaaataatta aagtcgcaac gaatgtcact gttagattac
     6361 cacgctggta gccttcctgc caagcaagac ctccaagtcc acctgcgccg atcgcgccgg
     6421 ccatcgcagt aaatccgatc atcgagatcg ttgtaactgt taatccagaa atcaatgcgg
     6481 gaatactttc aggtatcaag accttccaga tgatttccca gtaacttgct cccattgcat
     6541 ctgctgcttc cagtacacca gggctgactt cacgaaaagc aatctctact agacgggcat
     6601 aaaatggtgc tgcggataaa acaagagctg gaatggctgc ttgcgcacct agcatagtgc
     6661 ctacgattgc tttagtaaat ggaatgatca aaaccatcag gatcataaat ggcgttgacc
     6721 ggaaaatatt actgatgatc gatacgattc cataaatgat tcgagcatac ggtttgtttt
     6781 tgcgtccaat agagtaaagg atcaatccca aaactaatcc gatgatcgtc actaagacca
     6841 ttgagattaa tgtcatatat agtgtatcaa ggatagcctg attcatcgtt tccgggttga
     6901 tttgtttaaa atctagatat gtttctgaaa agcttttatc cacgatggat cacctcaatt
     6961 cctactcctt gttctttgat gaacacttct gcttgatcta attctttggt ttcaccagtc
     7021 atcaagacag tcaatgaacc aaaggaacct tctttcactt gtttgatctt tccatacaca
     7081 acgttgacat cgatgttata gcgtcgaatc gcttgggaaa tgactggctc gttggcattt
     7141 ctttcgctaa aggtcaaagt tgccaaacgg ccgcttggat tttctttgat gagttcagct
     7201 aatagttctg tcgaatcagc ttctggttcg atgtcttgtt gtacaaagcg tttggttact
     7261 tcttttttcg gttgtctgaa gacagtcaat acgtctcctt cttctacgac tttccccaac
     7321 tccatcacag cgactttgtt gcagattttg cggatcacat tcatttcatg tgtgatcaag
     7381 acgatggtca aattcaattt cttattgatc gctaacaata agtctaatac ttcttctgtt
     7441 gtttgcgggt ccaatgcgct agtagcctca tcacaaagca ggatctcagg atcatttgcc
     7501 aatgctcgtg cgataccgac gcgttgtttc tgtccgccgg aaagttgtga aggatatgct
     7561 tgtccgcgtc cttctaaccc gactaaacgc aacagttctt ctgcccgttc tttacgtttg
     7621 ctctttggta cgccagctaa ttccagcggc agctggatat tttcaagtac agttcttgac
     7681 cataacaggt tgaaatgctg gaagatcata ccgattttct ttcggaatgt acgtaattct
     7741 ttgtttttta attttgtaat atcttgatcg tttacgatca cttcgccgct cgtgggaagc
     7801 tcaagtccat tcaggagacg gactaatgta ctttttcctg cgccagaata gccgacgatt
     7861 ccataaatat cccctttttc aacgtttagt gaaacgtcat tgacggcttg gatgtttccg
     7921 tttttcccat tgaatgtctt tttgacattc gttagttgga ttaatgacat gccattcaca
     7981 tctttctctt ataatttttc aaacaaaaaa gccttcgccc tcttgcctta agcaaaagga
     8041 cgaaagcttg aacttccgtg ttaccacctt gatttaccgt tacttcgcaa taacagcctc
     8101 ttccagtact tggacttagc ttctttccgt atactgtcgc gctgtaacgg gcactcccgt
     8161 agtaagtttg cttacgctcg cttactctgc tctaagacca tcttccatct cattttcgtt
     8221 acccattttc agcaaacagg gctctcttca aacgtccgtg acatgtactc ttcttttcaa
     8281 agctttctcg tttcatatag gattgcttat gattatagca actggaagaa tcagtgtcaa
     8341 cttttttcaa gtgattttct gattttataa ttcgtcaaat tcttttggat caacatcttt
     8401 gaaacttaag atccacgcat tcatttcatc tgaatcattc aagatattga tatcttcacc
     8461 gattttatca ttaacagaag aaactgtacc cgttagcggg ctggcaaatt cattgaccgc
     8521 tttttccgct tcaacttcga tcagaggttc atcttttttt agcgattgtc ctgcttttgg
     8581 cagattggca aacgtgatgt ctccaagttc ttcttgcgct tcatttgtca aaccaacaca
     8641 gtattctttt ccattaaata agatccaaag gttgtccttt ttcttcaaat gcttgcctgc
     8701 cattttatat cagttctcct tcataaattt tttcattgaa tccaattgca acaagctggt
     8761 cctctcgtaa gatcagtggt cgcttgatca gcataccatc tgtcgataag acttggctcg
     8821 cttttgaagc cgtaaactcg cctacttgat ctttcagtcc aagttcccga tatttcatcc
     8881 cgcttgtgtt gaacaggcga cggattggta cttgactcgt ttctagccaa gtctcaatcg
     8941 ttgaggcctt cggtacttct ttgatcatat caacggtttg ataactaacg ccatgctggt
     9001 ctaaccacgc tttggctttt ttgcatgttg aacatttcgg gtaccagtaa aaggtgtaca
     9061 attttcttac ctccttgaag atcttgcagg tgcttcattt tggtatctca tcgataatat
     9121 caacccgata ccgatcatgt tccctaaaat cgaggatcct ccctgactaa tgaaaggcaa
     9181 tgggatccct gttaaaggaa gtaatccgat atttgctcca atattctcaa agacatggaa
     9241 cagcagcatc atgatcaaac cgcttgcgat ataggcgtaa aattcattat tcgtatcgaa
     9301 acacacccga atcatacgat agattaaaat aaaatacaag aaaatgacga atgcaccacc
     9361 gataaagccg aagttttcac cgataacggt aaaaatcata tcggactcac gaacaggcac
     9421 atagatattg cttacgttga atcctttacc aaacaagcca ccggtaccaa ttgccaaaag
     9481 tccttgggcc ggctgataac tcattcctga cgtatcatgg aaagggtcca gccatgaatc
     9541 aatacgtgca aactgataag atttgaatcc aactttataa agcaagtctc ttccgccttc
     9601 agtcgtaacc aagaagatcg ttcctgctcc aacgactact gccaagatta ctacaggtgc
     9661 gatgatgcgc caagagattc cagacatcag gaaaatcccg ccaaagatcg caagaaagac
     9721 aagcatcgtt ccaaagtcat cttgggccat gatcaatgct aaaaccggta ttgtcacgat
     9781 caagagcttc ccaatcagcc aaaagtccga tttcatcgtc cgttctttta cttgcgtatt
     9841 atgtttagtt acgatcagtg ccatcatcaa gatgtacgca atcttcatca actctgctgg
     9901 ctggaaagta aacgcaccga atcgaaacca gtttttagac cctgttgaag cagctaaatt
     9961 ctgatcatag aatttgagca agagtcccat taccactaac cccaaaccat aaaggtaagg
    10021 agtcagtttc cagatccatt tggaattcaa atgcatgatg acaacgattg ctgccgctcc
    10081 aactaaatac cagacaactt gcatccctac gcctttcaag actgctggat tgttcggatc
    10141 atggctaagt gctacatata atgagagcag accaatcaaa cataacagaa agactggtaa
    10201 aatcacgcca taatcaatac gattatcgtt cgaagctttt aatttttttt ccatgtttca
    10261 tcccttttct taaaaaccat actgctgatt attatagcgg tccagttaga taatggaaag
    10321 tctttccata gacatttggg aaaaattaac tttccgtttg tgcagttgct tgagcaaatg
    10381 cagtacaaaa gcctactgct tatctttagt gtatcataga actttttttg atacatgcaa
    10441 ttatctacca gctgagattc tcctcgctgt gagaagtttc tgtgctttca ttgtatcgaa
    10501 ggatctcttc ggcaatctct agcagctgat cagaaaccag attggttcgt tggatgaaat
    10561 aaaaatatcg atggaaattc aacggagtca ttggcacatt ttctggaaca gagcgcttag
    10621 acaaaatcga gcgaccgatt ccttccttca gcaacgcaac aattacatcg ttacttttca
    10681 cgatcatctt cttatcttgg atattcatct cttcaaaata acgctgcgtg taatgaaaaa
    10741 caccagagtt tgcttctctt acaagccaca atttgctttt tgggtcccca gcgatgacta
    10801 gctggtcttc catcaaagag tatctttgta cgccggaagt ctctaatggc ttttcgataa
    10861 aaccaaaatc ggattcatgt ttcgctactt gttctaatga ttcatgagaa ttcgtgattt
    10921 cgatctcaaa atgcacttta ggaaaccttt tgatcagatg tttcatcaac tgaggcaaaa
    10981 gatagacagc aaaggtgtgg gaagcaacga tccgacatac ttcttcttca ttttgttcat
    11041 tgagcaattt ttgctgagtt tcttcccatt cttctaacaa aaccagtgtc cgttcatata
    11101 ataagtcggc ttgcttagtt ggaagcatct cttttcttcc gttacgttga aaaagatcga
    11161 tattcaattc ccgctccaac tgtttgattt gattcgagac tgctggttga gataaaaaca
    11221 gtacttctgc tgctctggaa aaatttcttg tttcatatac tgctcgaaaa gtcgttaata
    11281 acttaaacat ggcaccactt ccataacttt ttatgataat tactataaaa actatttgat
    11341 ttaaatatac aaaaaaccat ctataataga aatgtacaac aaaacagttg ctgatccaac
    11401 tagtaactta gatgaagaca atcagctgtc tgcttgctcg taatcataca ttcctattca
    11461 ttttacaatg tattcgttca tcttggtact aaaatctatt aaataaaaag gagaatatta
    11521 tatatgaaga actttttgaa aattattccg atcccgatct gcgggttgat ccttgggtta
    11581 gcttcccttg gaaatctgtt taaagactat caccatgttg ctttaggcaa tctaactggc
    11641 ggtatctcta tgattcttat gatattcatt tttgttaaat tgtttatctt attcgaacac
    11701 acgaaacaaa cgttgcatga tccaatcatc gcctccgttt caccaacttt tacgatgtca
    11761 ctcatggtta tttgtactta ttttgtttca tatgaaagta ttgcaccaat tgtcaaattc
    11821 atttggctgt gcgctgttat cttccaagtg atccttgtct tttatttcaa ttatcaccat
    11881 gttatcaaag ctgatctttc aattgaagca atttatccaa gctggtttat catttacgtt
    11941 ggatttgggg tcatcactgt aactgccggg aacttcttcc cacttattgg aaaaattttc
    12001 ttttggattt cattgatctg ctatgcgttc ttattgccga tcatcatcaa acgtgtcttt
    12061 ttcgtaagaa atatggctca tccgacacgt ccgctgatta cgatcgttgc agcacctggt
    12121 tctctttgcc taacaggtta tcttaaaaac tttgctgaac ctaattttta tcttgtcgta
    12181 gcattgttcg ctttatctca agtattgtat ttcgttgtgc tgtctatgct tccacgtttg
    12241 ttcaagcttc gtttttatcc aagctatgca gcctttacat tcccgcttgt tatctctgca
    12301 acagctatat ttactactgt tcattacttc aacagtttag gtatctatag tagcgtattg
    12361 gatttcttaa aagttgcgga atgtctgttt gcaagtgtag ttgtcttcta cgttcttgct
    12421 cattacgtac gtttcttaat taaagaacat agcaaacatg gaaaatttgt ttctaaaaaa
    12481 atcgcttaat aatcgattca ataggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    12541 cggccaaaca aaaacagctg ttcatgtttt tgtactggtt gggcttatta ccgcagatgc
    12601 aagcttttga acaccgttta ccaagaggtc gtaaaagaag tggtcagctt caagaaataa
    12661 gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa tttcggctta tttccgtaga
    12721 agctacttct ggaacaccgt ttattcgaaa caagaggtcg tacaagactt ttgtcacgac
    12781 ctcttgttct tttgtcaaaa tactggttta gtgctactat gttcgagtat gcttttagta
    12841 tttattgacg aaaggaaccg atcaaatgaa atcattgtca catccaataa aatctgtaaa
    12901 ggattcagcg gtccgcgaaa tcttacccgg tttactgctt tcttttgcca tcgctgcctt
    12961 cagcaggctt cttgctattt atctgccaca gttaggtgga gcgactattg ctattttatt
    13021 gggtatttta ttaggaaata ctttttttaa acagcctgtt ctagcaatcg gcacaaaatt
    13081 tgctgaaagc aaactattag aattctctgt ttttttacta ggtgcaactg tcacagctca
    13141 aacgattgct caaattggtg taaaaggaat tgtttttgtg ttactgcaaa tgtcagtcac
    13201 tatcatcggt gcttacttga ttggtaaaaa actgcaattc agcgatactt cttctttgat
    13261 gatggctgga ggaaacgctg tttgtggttc ttctgctatt gcttcaatcg ccccttccat
    13321 ccatgcgaaa gaggaagaaa aaggacagat catcacacta gtcaatctac tgggtactgt
    13381 gatgatgttg actctccctt ttcttggatt acagttgttt ggtggaaatc tcattgaaaa
    13441 aagtgcactt ttgggtggta cactccaatc tgttggtcaa gtagtcgctg gggcaagctt
    13501 acttgacaat tctgttgtcg aattcgccat gctatttaag attctacgta ttatgatgct
    13561 cgttgttgtt gtctatctgt ttgaaaaatt catgcacaag aaacaaccag ctctgtttga
    13621 aacgccaact gatacgcaga aaaaacgaaa attaccgctt ccttggtatg tattagggtt
    13681 tgtgatcttt tgtttggtca acagcttgtt ttctttacct gtagttattg accaatcggc
    13741 acattttatc agcagctggt ttgagatcac tgctttagcg gcaattggtc tgcgtttaga
    13801 ctttgcaaaa ttcatgaaag aaggtacacg cttcttatct tatggattag ctgtcgggct
    13861 tctccaaaca gtgatggctt tactatttat ttggctgatc aaagtttaga atgaacatga
    13921 aaaaacagtt gtgattctcg catggaacaa tgtccgaatc acaactgttt ttaattttgc
    13981 agatatcctt ctaacaacaa caattggcgt ttgatttcaa ccccatctat tgcgtttccc
    14041 atatatcctg tcagttttcc attttgcccg atcactcgat gacaaggaat aacgattggc
    14101 aatggattgg ttcgatttgc ctgcccaatc gctcgcacag ctttcggact atcaacagca
    14161 acggctactt ctttatacgt tcggatttct ccataaggaa tggtcgacaa agctttccag
    14221 acattttgct gaaaaggcgt acctctcttt atagataaag gaacatcaaa ttcttttctt
    14281 tttcccgcaa aatattcttc aaactgttct tgtgtgcgcg ttaaaattcg ctgattcgct
    14341 gattcgcttt ctgaaattgg ctgaaaagat acagcagtca atccggattc atctgcatct
    14401 aaccataaaa agccgatagg tgtgtgtatt ttcattttgt ttgctcctag tcaatagact
    14461 ggaacttcct cctttttctc tatccaaaac tacttgccaa ctaataaaaa ttgttcttaa
    14521 aagtttgata aaccgaggaa acatggtaaa ctaaatacgt tagtttacaa aaagaatacc
    14581 tgttctttta cgtaaaatca aattttaaag gtgttattta aggtttatta aattttgaat
    14641 gtaaaccata tataaacata cgattgaaag gatagaaacg atgaaagtat caattgttgt
    14701 cccatgtttt aatgaagaaa aaactgttcc tctttttttt gatgaggttg aaaaattcaa
    14761 aggtgcccat gagttcgaat atttgtttat tgacgacgga tcaaaagacg gtacgttagc
    14821 agcaatcaag aagctagcct cagcttatcc ttatgtccgc tttgtctcat tttccagaaa
    14881 ttttgggaaa gaagcggctc tgtacgctgg attgcaagct tctacaggag atttagtgac
    14941 cgtgatggat gttgatctgc aagatccgcc cgaactgctt cctgagatga tcaaccggat
    15001 cactacttct gatatcgatt gtgtcggcac ccggcgttcg actcgtgaag gcgagcctgc
    15061 gatacgcagt ttctttgcga aaaaattcta ccagttgatc aataaaatca gcgatacaga
    15121 aatggtcgat ggcgctcgtg atttccgctt gatgactcgt caaatggtgg atgccgtcct
    15181 agaattaaca gaatacaacc gattttcaaa aggattattt agctgggtcg gatttaaaac
    15241 agaatatatt tcttatgaaa accgcgaacg tgtggctggc gaaacatctt ggtccttctg
    15301 gaaattattc aactactcga ttgatggtat cgtgaacttc tcggatgcac cgttaacgat
    15361 tgcttcattt atcggcgctt tgtcttgtgt cggttctggt attgcaattt tgttcattat
    15421 tttgcgggca ttgttgtttg gtgatcctac atcaggatgg ccttccatgg tctcgatctt
    15481 cctgttcatt ggtggaatcc aattgttatg tcttggaatc atcgggaaat atattgggaa
    15541 aatcttctta gaaacgaaac agcgtcctgt ttatatcata agagaaacag aaaaagtgaa
    15601 gccaaatgaa ttgaagcctc accaataata tacgtcaaat gaagtaaaca tttttttaaa
    15661 tgaaaaagga ctgtgacaaa agtcttgtca cagtcctttt ttccgaataa atggtgttcc
    15721 agaagtagct tcttcggaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt
    15781 aaattccttc ttatttcttg aagctgacca cttctatcac aacctctttc atatcttatt
    15841 acttcttcat tgctgctttt agtgctgctg ctaatgggct ttcttctggt tcttctgctt
    15901 gtgcatattt tttcagcaag cgtcgttctt catgtttggt cattttcttc ttctgctctt
    15961 ttttatcaag caatttttct gtcgttccat cgtatttaca acggaaataa gcaccgtttt
    16021 taccttcgat gatttccatt ttccggtgac attgcgggca tcgatgatta gagacttttg
    16081 gatcttttct tctgcggtag ttgcaatctg aattggtgca gacatagatt ttgccgtcac
    16141 gtgtgttttt ctcgcgcaga ttggccccac attctgggca tttcttttgg gtaatcgaga
    16201 aatcttgata ttcttgtttg cttgatttga tctcattcac caagcgtttt gtttcttttt
    16261 cgattccatt caagaaggtt cctgcttgtt tctttcctgc agcaatagct tcaaggtctt
    16321 tttcccagct ttctgtcaaa tctggtgtta ctaaagaggg atttaccaat actaataatt
    16381 gcttaccttt aggagatact tgcagtcctt gaggggtacg ttccatcagt tcagatttga
    16441 ttagtttctc gataatctct gcacgcgtag ctggggttcc cagactgtat ttttccattt
    16501 gaccaagcaa gcttgcttct gacaatggtt taggcggtgc agatagttct tttttgatgg
    16561 cgaaatcggg attgattctc attccttttt cccatttgac tgtttccgtc tcagtttcag
    16621 tttcgccttt ccatcctgct tggacgacac gattttgccg aaaaatgaat cgttctttac
    16681 caaattcaac cgtaactttt gtttggctga ctttatgggg ttgagcaaac agtcctaaaa
    16741 aacgagagac aatcatttga tagatttttc gctcttcatt acttaatttt tcataacgtg
    16801 ctctgttttc agttggcaaa agtgcatggt gatcggtcac tttactgtct tggaatactt
    16861 ttgtctgttg gacgatggct ccgtttttca aatactgctt gacctcagga gcaaagtcgc
    16921 tgactgcttg caatcgttct ttcatcgtac ttttcatgtc atttgtcaga tatttgctgt
    16981 ctgtacgcgg ataagtaacg atcttatggg tctcatacaa actttgtaca agacttaatg
    17041 ttttctttgc agaatagcca tagcgttgat tggcttctct ttggatctct gttaaatcat
    17101 aaggcaatgg cgcagattcc gtttttaatt tttcttggac ttctgtgaca gttcctttgc
    17161 ttgtactgat caattgaaca agtttttgtg cttcttcttg tgtttttaaa gcaaattgat
    17221 ttttttgaag cattttggct tgttcttttc cagcttgcaa cgtgatcgtg aaatacgttt
    17281 gtggacggaa ctgctcgatt ttcttttctt ggtctctcac taatgctagt gtcggtgtct
    17341 gcacacggcc tgctgataaa ttatccgcgt acttcacagt taaggctctg gtcacattca
    17401 atcctaccag ccagtccgct tttgcccggg caagagcaga ttcatacaat gcatcatatt
    17461 ctttcgcagg tcttagttgc ttgaatccag ttttgattgc tttatctgtt tgagaagaga
    17521 tccataaccg cttcactggt ttattgaaat gcacatactc taagatccag cgagcaacaa
    17581 gttccccttc tcttccggca tctgtcgcaa tgactgcttc tgagacatct tttcgctgag
    17641 ccagctgctt gattgctttt aattgatgtc ctgttttagg aagtggtttg attcctagtt
    17701 ttttagggat cattggcaac gtgtccattt gccagctctg ccattcctta ttcagatctt
    17761 ccggcatttt caatcctaat aaatgaccaa gggcccaagt aacgatcacc ttgggtcctt
    17821 cataataatt tttgtgcttt tgattagcac caagcacctt gcttaggtct ttcgcaacgc
    17881 ttggtttttc cgctagaatt agttgtttca taaaattcct ttctgctttc tactgtgatg
    17941 ggctgatgat ttttcaacaa cgcaagtccc ttgtattgct gtaaatcatc gtcacactct
    18001 tcacaccagc gctcatctcc gtcattccaa aaggcgctga gaaagttact ttttgcattg
    18061 atatattcat aattttgatg gatttcgttc cattgttttt cgtacgcttg tcgtgcttct
    18121 tcgaaagact cgaatgtttc ttcttcttca atatcttctt cccagtcttc aaaaaaccac
    18181 caaggctcat tgtccccgaa catggtaatt acttgataca tactcgtgct ccttctttca
    18241 gcttttctag actcatctta gtcacttttc aagaaagttg caagaatttt ctcttataaa
    18301 agggtcattt aatgaacatc ttaaaattgt tacaaatgaa tctgattgtt taaggcaaag
    18361 tagcgataaa gttattgatc atccaaaaat agctgtcttc tggatcgatt gttggttctt
    18421 ttgtaaagta tcctaaaaaa cgtaaggaaa gataaccgtg gatcatacta cgaagttccc
    18481 tgcttcgatg aactttttct tctttcgtta attgaaaagc ctcaaatatc tgcaaaatga
    18541 tctgattcgt ttcatggatt ccatctaata aaacagcttc attggtatgc ggtacactga
    18601 tcagtaactc atacactgct tgatttgaaa aagcaaagga tcgataagtt tctgcataga
    18661 tctgcagtgc ttcctgacca ctttttccca caagagcccg tcgcagctgg tcattaagtt
    18721 cctgcagaag tttcgctgtt agcgctgttt tgacatcacg aatatttttg aaatggttat
    18781 acagcgaggg agccttgata tccaagcgtt tggctaaatg ctgaaaggtt atttgctgaa
    18841 catccatctc ttctgctagt tctcgaaagc agtcgatgat tttctcttga gacagatttc
    18901 tttttttcat ttccatgttt cctctcataa aataaatcct tcactctttc agtttaccac
    18961 gaaaaagaaa gagaaaaaag agactttcat tgataaacta catattgtag tttataataa
    19021 agagctgtag atgaaaattt tcagaaagaa ggagtctaat tggctgcaag aataaaggaa
    19081 aaattattta ggaaaagaga aacttgggaa caaaacttgt ttgtcttatg gtttggaaca
    19141 tttatggctg gtatcggatt tagtttagtc atgcctttta tgtcacttta catagatacg
    19201 ttaggtaatt atagtacgtc acaattgaac ttttggagcg gattgacttt ttcatctact
    19261 ttcttggtga ctacgcttat ctctccttgg tggggaaaat tagccgacca aaaaggaaga
    19321 aaattgatgc tgttgcgtgc ttcattagga atggcaatcg tcatttcttt gatgggttgc
    19381 gtgactagtg tgtatcaatt agtcgcattg cgtctgcttc aagggatttt ttctggttat
    19441 atcagtaatg ccactgccct tgtcgcaact gggacgccaa aagaaaaaag tggtcaagtt
    19501 cttggaacct tagcaactgg ttctgttaca ggaacattac ttggaccgct tcttggcggg
    19561 atctctgctt cagcgtttgg ctatcgccct acctttttca ttacagggat catcttattt
    19621 ttcgtcttct tgttgagtct gtttttcgtc catgaaaaat ttgtcccagt gaaaaaagaa
    19681 aacatggttt ctgccaaaca gattttcaaa gacttgaaat atccacacgt cgtcatcggt
    19741 atgttcgtca caacgatgat catccaagca tccaacaatt ccattagtcc aatcatcagt
    19801 ctgtacattc gccaattgct gcatggacat gggaatgtta cgttgatcag cggaatcatt
    19861 gcttcgatac ctgggattgc cactttgata gccgctccaa gatttggtcg cttaggtgac
    19921 aagattggga gtgaaagaat acttgcgatt ggtttgggtt ttgcgattct ggtttatatc
    19981 ccaatggcat tcgtcacgaa tgtttggcaa ttagctgcct tacgtttttt gattggtatc
    20041 tctgatgctt gtctgctccc tgctgtccaa gcattgatca caaaatattc gcctcatcaa
    20101 gctgctggac ggatcttcag ttataaccaa tctttccaag caactggtaa cgtagtaggg
    20161 cctatgatcg gctccagtgt ttccagtgca tttggttatc gcggcgtctt tctttcgact
    20221 tctcttcttg ttttggtcaa cttcttgctt gtacgacaca atacacaaga gatccatcag
    20281 caagaaactg cagaagaaaa agcttcagga gtctccatag cttcagggca ctctccttct
    20341 catagacaat aagcgtaaat gaaaaacact gcttgaacag ttgtcacaga ctgtccttgc
    20401 agtgtttttg tttaggcctg ttttttcaag aactcagata agaggaataa tcccttttcc
    20461 aaggtttctt ttttacaaca gtaacctagt cgggcatgct tcggtaaatc aaaggcactt
    20521 cctggtacta atagtactcc tgtttcttct aataaattga tacaaaatgt ctggtcatct
    20581 tcttcgatgg ctaatttaat gaaagaagta gagacgtaat tgggagcgac aagttcaact
    20641 ttcggttcat tggctaccca ttgttttaaa atcacaaggt tctctgtaat gatctttttg
    20701 tttcgcgcca atattttttc tttattcttc aatgcatgta cagcaagatc atctgacagg
    20761 actccgccgc aaatcatcgt gtagtctcga tactttcgaa aaacttctgc caactcaggt
    20821 ccagtagctg tccagccaat acgaatacct ggaatggagt aggttttgga tagggaattg
    20881 gttgctatac ctttttcata gacatctacg atcgacaaga attcaccttt atccgttaaa
    20941 ggcgcatata cttcatctat caatacatat gcatctactg tacgagcaat ttctgcgatt
    21001 ttttctaaag ttttccgatc tagcaatgtg cctgtaggat tgtttgcact attcaaacag
    21061 atgatttttg tttcaggttt gactaaggct tctagctgtt ctaaatcaaa ctgccaatct
    21121 tcttcttcct tcaaatgaac aaagtcaaca gcggcaccta aagatttggg gatgtcatat
    21181 aattgctgat aagtaggaag catcgaaaca acatggtctc ctgcttccac taacgcgtat
    21241 agagctaaaa gattagcacc agtcgctcca ttcgtttgta agatgttttc cggatcgacg
    21301 gtttgataaa gagaagcgac ttcttgtttg aaaacatctg atccttcgat ccagccatag
    21361 tccaatggtt cgtttttgcg tttttcgaaa aatttactta cgctagtccc atctaagcca
    21421 acgagttcgt ctaatgtcaa tgcttcgatc gagctttgtg caatatcata taccgctttc
    21481 ttttcccatt tatttagcca ttcttctaca ccgaaatttg cgattttcat gtttctttcc
    21541 cccttatatc ttgatccagc ctacagcaag caacccgatt gaaagcagtg cggccacaca
    21601 aatcagccca atcattactt tttcttttcc cgtaatttga tgtttggttt cacgacaagc
    21661 gatccagtag aagcaaaaac caggaagata ggcaatacta cataaaagga cataagaaaa
    21721 tcctgctaaa atgatgccca tcaattcaaa aactacagca atcccaccga tcactacttg
    21781 ttttttgatt ccttctttta aagaaaactg gacttgatag atcccgacaa gtagataaca
    21841 aatcaaaatt gccgctgtac ataaagaata ggcaaattga taggcataat cagtaactaa
    21901 gaaagtcaat aagaatagat tagtcaagat tgcagtaaca actaacgaat aagttggggc
    21961 atttttttta ttcgtttttc cccaaccttt cggcaacaga ccgtctcgag ccatcaacat
    22021 cgttgtttct gctgggagca tcgtccaaga caaccaagaa ccaatgatcg aaataatcaa
    22081 gccgatattg atcagcgctg ctccccaatt tcccacgatt ccttttaaaa tatttgccat
    22141 agcaggttgt gaaatagttg ctaactcttc ttgagataag acgccgtatg gcaagatcga
    22201 agcaaggata tagataaaca ataaacaaag cagaccaatg atcgatgctt gctgggcttc
    22261 tgaacgtttc ttcgcgcggt tggctaaaac acttgcgcct tcgatcccta caaagaccca
    22321 catcataacc atcaaacaac ctttaatctg caaccagatc gttccttgct gccctgttcc
    22381 attcactaga ttatcagata cgttacccca aaaatcagca gtgaatatat tcactttaaa
    22441 tgcgataaat acaaatatta aaaataggaa taatggcact aatttacaga tcgtcacgat
    22501 tgtattgatg acgctcgctc cttcaactcc attgtttact aataaagtca gaccccaaat
    22561 aaaaatactt gcaaataaaa tagaaggaac attttgtcca cctttgaata tcgggaaaaa
    22621 ttctcccatg gcgctcatca gcatcgttgc aaaagcaaca tttcccagcc acgcagacaa
    22681 ccagtaaccc caaccactga tgaagccacc taatttacca aatgaagctt ccgcgtaacc
    22741 gaaaatacca ccatctagat ctggacgctt ttctcctaaa tgattcagcg ataataccaa
    22801 agctaaaata ccaatcccaa cgattatcca tgaaaggatc gctggccctg gagctgcaga
    22861 actagccaga tcacttgtga tcccaaacac accgcttccg attgacgaac tgacaaccag
    22921 agcaaccagt ccccatttgc taatacccgt cgatttcttt tccatttcca tcaccctcac
    22981 ttataaaatt gaacaaatca cttgcaaaag attcccttct attttgcaac tgctcttgct
    23041 taacaaaaag caactttatt catattattg gatattatga actttttaca caagtatttc
    23101 aagtataaaa tgaataaaaa ttaattttat tgcaaaaaaa cagctatgct atccaaaaga
    23161 aaaggtgttt cttttgttag ccatagctgt tttgattcat ttaatatcgc ttaaacgtat
    23221 tgtaaaactt ttgtttcttg atatgcgtgg tcgatcaaac caccacccag acattccatt
    23281 ccatcataaa atacaactgc ttgtccagga gtgattgcac gtacaggttc gtcgaatacg
    23341 acttctgcac gattatcatc caataagcga actgtcacag gaacgtcttg ctgacgataa
    23401 cggaatttcg ctgtacactt gaattctttt ggcattggtt cattcgtcgt aaagtggatt
    23461 tcacttgcat ccaagcttgt ggcatacaat gcaggatgat ggaacccttg ccccacatat
    23521 aatgtgtttg tcgctagatc ttttcctaca acaaaccagg gttcttgcga atctcctccg
    23581 ccaccgatcc ctagtccttg acgttgacca atcgtataat acatcaagcc agcatgttgt
    23641 cctttgactt caccatcaag agtgaccata tttccttttt ttgctggcag atagttgctc
    23701 aaaaattgtt tgaagttctt ttcaccaata aagcagattc ccgttgaatc ttttttctta
    23761 gcagtcgcaa ggcctgcgcg ttcagcaatt gcccgtactt ctgatttttc catgccgcct
    23821 agagggaaca tcgtttttga aagctgttct tgtgataatt ggctaaggaa gtaagtttga
    23881 tctttattat tgtctactcc acgaagcatg tggaccacgc cgttttcatc gcgagtgact
    23941 tgcgcataat gtccagtagc tacataatcg gcgccaagat ccattgcata atccaagaat
    24001 gctttgaatt tgatttcttt gttgcacatc acgtctgggt taggtgtacg tcctgcgcgg
    24061 tattcggcta ggaaatattc aaatacacga tcccaatatt ctttttcaaa attcacagag
    24121 taataaggaa tcccgatttg agcagctact ttcgctacat ccttataatc ttccgtggcc
    24181 gtgcatacac cgttttcgtc cgtgtcatcc caatttttca tgaatattcc gatcacgtcg
    24241 tatccttgtt cctttaacaa aagtgctgtt acagatgagt cgacaccgcc gctcatccct
    24301 acgacaacgc gtgttttgct gttgtctgtc atgatatcac catcatttca attagattct
    24361 gaaacatcta cgagttgaag gtcgcaactt ttcagtattt tacattatac gtcatatttc
    24421 tgaaaatttc atcccttaat tatcaagtcg taaaaatact gttctactgg acctattcag
    24481 tacacaaaca tgatagtcaa ccaaaaaatc actcatcttt tgagcattcg atataaaaaa
    24541 ttggcatact cataaaaagg gccagccttt cggcaataag gaataatttt ataaattgtg
    24601 aaaaactatt tttcaaaatt gcttcttatc gcttaaagct gcacgtctca tttacaacct
    24661 tttcttttta gatgttatga gttgctcaga taagtgattt tgtttactct aagaaaaaag
    24721 ggaggcaaaa gatacatgaa ttactttatc agtgatatgc actttttcca tgaacgctta
    24781 ttaggaaaaa acgactttgc tccgcggctc tttgcaaatg tggatgaaat gaaccaacag
    24841 ttgatcacta gctggaatgc agtaataaaa gaaaccgacc atgtttatca tctaggcgac
    24901 cttgctatgc atccgcaata tgaaaaagca aatgcagaaa ttttggaact ggtcactcaa
    24961 ctgcaaggaa caatccactt gatcaaaggg aaccatgaca gtcgcgcttt tttccgttat
    25021 ttagaaaaaa atgatcctag gcttactgac ggaagtccca aattttattt ttatgatgta
    25081 ggtgccatcg ttaaattcga ccatcaacaa tattacttga ctcattatcc gatgttgctc
    25141 gggcctaacg acactatccg caatcttcat ggacatatcc atcattacag cgtacctatc
    25201 gagaacgatg tcaatgtagg cgtagacgca ccggaaagag aactcttaaa ggaaaaacta
    25261 ccatttggca ctccgttatc tgagcaacaa atcaaagaaa tctatgaagc aaagaggaag
    25321 tacgataggt cgtgaaaaaa ctgccctata tcaagtatta aacaaataaa gataattact
    25381 catatttttc atttgagtag gcttattgcc acaggaacat taggatcaga aatggagttg
    25441 taacaagact tctgtcacaa ctccattcca tactattcgt ctttttgctc atggtgttct
    25501 tttaccagtt ttttctgtaa ttctttatca taatttcggg cagctgacgt taaaaataaa
    25561 acgattccgc ataataaaat cccgattccg gaaaacaaga acattttttc tacccctaaa
    25621 cgatcagcta atggtcctgc aaataataat ccaattggac ctgccaggtt catcaaagag
    25681 ttgaagttcc ctaagacacg acctaaaaca ttcgattcat aactctgctg gatcattgcc
    25741 atcagcagtg tattgaaata tggcgttgca caacctgctc ctgcattcaa gataagaaag
    25801 tacaagaacc cttgactatc gtttggaacc aatccgcttg caccgatcgt caggcccata
    25861 atgatgtagg ctataataat aggtttcatt cggtctttcc atgttcctat aaaactgatc
    25921 agtgcaccgc ctaagagcat tccgactgcg tagactactt ccactgctcc cgcctgtcca
    25981 acggaaccac caaagtaatc tagcgtcatc aatggataca gactgattgc tggcatgaac
    26041 aacagcatca caaatgcacc attgatcgtt atgtaccata accctttatt atccatcaat
    26101 tgctgcagcc caaattttgc atctttaaaa atagtaagcg gaacttccaa tatttcagga
    26161 gaaacttttg ggatcttcac aaaaatcaat aagccgacac caaaaactgc ccccaataca
    26221 tctaataaaa taaggtaatt gacaggaacg actgaaaaca aagccgcacc tagagctggc
    26281 gcaatgatga aattcgctga ttggaccatt cctaactgtc cattcgtcct tgttatgtga
    26341 gaagatggaa cgattgtcgg cataatcgac tggatcgttg gcatttggaa tgtttgtgcg
    26401 accgaacgca taaacaaaga cacaaaaact agccaaatcg ggaaggattc tgaaatcgtt
    26461 ccgactactg ctaaaataag cgcgaaaatc gctacaataa tatccgtgac aatcaaaaga
    26521 gcttttttat cccagcgatc cactagcggt cctacaaatg ggcttagtaa aaccatggga
    26581 atcattccta gtaaagtagc aaaactcaaa attgtcgccg aaccggtttc tcttgtcaaa
    26641 taccagatga tcgcatattg gacgaccata ctagtgattc ctgacaaaaa ctgtcccgat
    26701 aaaaagaaca aataatttct cttccaattc gttaaaacga ccgactgttc attttccata
    26761 aacatccctc ttttcttttt cattttgtat agcgtttgca caacaatcat tatacgggat
    26821 tcttttttca aaaaatacct ttttccctaa ataacaatcg tttcaattta aatttcttta
    26881 cttagaaaca ataaatcagc aaaatattaa aaataacaac atgtgttaaa tttaaaaata
    26941 aaaaagacaa ctaatttgcg tcgtccttac aaagttagtc atctaacaat aggccgtttc
    27001 aaaactagtt gtctttttat atccacagat cattcgactt tttcgaataa cccacggcta
    27061 atcatcccat ccatcagaaa ataaaattta tcttggctca ttttctgttt tggatctttg
    27121 aaaatattga ctgctttcaa tccatttgct gtagtgatcg acattttcaa tgtttgaata
    27181 tcttttgata cagtgatttt taatttgaat ccttctttgc tttgcggcgt ggcatctaat
    27241 ggtcggatca attgataatt gcctccgttt gtttctgtaa atccattatc gcgcaatgta
    27301 tatctttttg catcaggatg caaacgatag ctaattggtg cgcccagtac cgttgctgtt
    27361 gtttcaaatg ccatgatttt cacctcgata aaattagttt ctctttatta taaaagttta
    27421 tcctgatttt tactaggttt ttcagttaag ttccctaaaa aatgaaggcg ttctctcaga
    27481 catctgttca tataagaatg acttcactag ttattttttg acagcacgaa ctaattgatc
    27541 aaggaaatac tggatgtcgt cttcggtatt tcccaaacca aagctgatac ggatggattc
    27601 ttttagtatc ggtgtatctt ctccatacat tgcttctaat acgtgtgaag gctcgatatt
    27661 tcctgctgta caagctgaac cagtagaaat agcgacccca ttaagatcaa gcttcatcag
    27721 tagcaaatca tttgcttcgc caattaagcg aagattcaac acatgaggga gtttattcgt
    27781 taaatcacca tttacttgat aagggatttc gtttgcttct aatgtatcaa gaatcaattg
    27841 ctggaatcct ttatatttgt tgttacgtgc ttcacgttct tcaggtgaca gcagttcaac
    27901 tgcttttgcc ataccccaga tccctgctaa attttctgta cctgcacgcc gcttttcttc
    27961 ctgctccccg cctaaaagca atgcagggat atttactccg tctcgtttat ataagaaacc
    28021 gacgcctttt ggcccattga ttttatgagc agagatactt aataaatcga ttcctaattc
    28081 ttctggatgg atcgtttggt ttccgtacgc ttgtaccgca tctgtatgga aataagctgg
    28141 atgatccgct aaaagttcac cgatttcttt gattggcatc aaatttccga tttcattgtt
    28201 cccgtacatc acagagacca agatcgtatc ttctcgtaac gcttttttga cctcttcgat
    28261 cgacagattc cctttttcat ctacaggcag atatgtaacg tcaaacccct ttgtctctaa
    28321 atatttcatg gtattaagaa cagcaggatg ttcgatagct gtagtgatta gatgtctgcc
    28381 ttctttttga cgcgagaacg ctgtttctaa gatagctgta ttatcccctt ctgttccccc
    28441 actgttaaag atgatctcgt gaggttttgc ttgcagactg tctgcaatga tttgtcttgc
    28501 tgtttccaat ttttcatgcg cgtggcgacc aaatccatgt atgcttgaag gatttccaaa
    28561 cgtgtcttgc atgattgccg tcatcgcttc aattacttgt ggatgcatgg gggtagttgc
    28621 cgcatgatcc agataaatag ttttcacttc attcatccct tccaaattat tgctaggcac
    28681 attctagcat aaaatagcga acaaaccaaa gaagagctca tttttgtcta gaggacgttt
    28741 catgggcata gaaaaaagga gctgtgacaa aagtcttgtc gcagctcctt gaagctgctc
    28801 acttctgtca caacttcttt ccctcttttt tttgtttatt cgaattcttt ttcttccaga
    28861 cggaataacg gactcatcgg tgtattttca tggatgcgtt tgaccgcttc acccattaat
    28921 tctgaacatg taacgattgt taagttttct ggatgacgat tttcttctgt gaaaacagaa
    28981 tcagtgatac aaatatcttt gattggtgca ttgtctaaaa tacttttcgc atgatcggac
    29041 aataatccat gtgacgcaca ggcatatact tcttttgcgc cgttttcttt taatacattt
    29101 gctgctcgtg caagcgttgc tcctgtgttc aagatatcat ctaccatgat acatgtcttt
    29161 ccttgaacat caccgatcac ataaccaccc tcagttgaag catcatctgc atgatctacg
    29221 atggctaatg tgctgttcaa atattctgaa agactacgtg cacgctgaac accgctgttt
    29281 tttggcgaaa caactacgat gtcatctccc gtcagaccta attgacggta ataatgagca
    29341 aacaacggca tcgtaaataa attatctaca ggaatatcaa agaatccttg tacttgaacc
    29401 gtgtgcagat ctaaagtcag tactcgagtt gcccctgctt cttcgatcaa gttagcgatc
    29461 aatttcgcag tgatcggttc atgaggtttc gccgtgcggt cttgcctagc atacccatag
    29521 taaggcaata cgacattgat cgttttcgca cttgctcgct tcattgcatc gatcatgatc
    29581 agtagttcca tatagtaatc attgacaggt gcattcgttg attgaacgat atacacatga
    29641 tcccctcgaa tactttcttc gatattgatg gaaatctcgc catcactaaa ttgcttcacg
    29701 acgcttttac ctaattctgt tccgaacact ttcgcgattt tttcagctaa tggacggttg
    29761 ccattcaaac taaaaatctt caacgtgtca tcctgatact tattactcat gtagtcctcc
    29821 ccaaaacttc tctattcttc gatagtttaa catatttttc caagattttc tgttaatttc
    29881 agcaaattct taaggaagtg aaactgttcc gttactccaa ctggcaggaa tgactttatg
    29941 ataatcgccc cagttgtcgt gtactttgta ataacgttct cctgtttcag tttcaaaata
    30001 agcgtacgct gtcacccaat gattgccata tgtactgcca agtactttca aaataccaat
    30061 catcacaggt ttgccttcac gtatacgttt tgtagctcgc tgccatgaac ctagaagagt
    30121 tgctcgtgca cgctcatgat tgccattctt ctttaaaaaa ctagaaatcc ctgtactaac
    30181 ctgaaaagga atcgtaggaa gtcctagtgg ttggatctgg ctacgcaatg ccttcgtcaa
    30241 aaccaacgaa tcagccgtat ttttcttgcg aatctcattt ggaagcatcc accccttacg
    30301 atagtcttga taataggcaa ggagaactgc tgccgcgtaa gtaccacaaa gatagcctga
    30361 gggggtagcc catttcttat acctcaaaaa acgttcttct ggcaatccca cccagcaatt
    30421 tgcgagttcc tttcctgacc ttttttcttc gatcaaaacg ggaatatctt cttttctgtt
    30481 gatccatcta gataactcga ttggctcttc attgatcagc ttccattttt tacaagaaag
    30541 accctcttga taagtatatt ttgctggaga atattctacc ggatgtcctg attgatcgat
    30601 aatcacataa ctttgatctt caaaaaccag agctaaaaaa agcaatttac tttttcttga
    30661 ctgcagctgc tgagtgctgg ctaatttttt aattttttta tctactttga aatataaagg
    30721 tttatccact acttgattca gttgggatcg cctccttttt ttcattataa gtgaaggatt
    30781 gaaaaaaaca aatcagtttg attgttcgaa ttttcaattt tttatgttct atttgttaat
    30841 tccttcattt tgtaagataa tgggcacacg aaacaaaaaa ggagcgatcg atgaatgaac
    30901 aaagaacgat tgaaacaaga tttctatgaa gcagtcaatg aagaatggca aaaaacagca
    30961 aaaatccctg cagataaacc agctacaggc ggtttccaag atttagttga cgggatcgac
    31021 caacttttga tgggagaaac agacgatctg cttgcccatc cagaaaaagt gtcttcagaa
    31081 aaaatgaaac atttccttgc atattatcaa ctggcaaacg attatgaccg aagaaatgag
    31141 ttgcatgctc aaccgcttca acctataatc gaaaaagttg aatcattgca gtctttcgac
    31201 gaattgaata agcaatttcc agaatggctg ttggatacat taccattgcc atttaatctg
    31261 gatgtagatg cagatatgaa gaatgcgcaa acgaacgcct tctttgctta tccgccttct
    31321 ttattccttc cagataagac gtattatgca tcagaccatc ccaacggtgc acaactaata
    31381 ggcgttttct ttgatatggc tgttcagtta ttagagcttg ccggcaaaga aaaaagtcag
    31441 gcagaagcaa tcacggaaca agcagtgcaa tttgataaat tgattgctcc tcatgtaaaa
    31501 agtgcagaag aaaacgcaga ttacagcaaa atgtataatc cgcaatcttt tgatacattt
    31561 attaaataca cagataaact ggacttgaaa aaacttgtca cgggtttgat tgggacaact
    31621 ccagataaag tgatcgtaac tgatccagtg tacttcgatc atttgaatga catcctgaca
    31681 gaagctcatt ttccgttatt caaaagctgg atgatcatta aaacagtacg ttctcttagt
    31741 ggttatctat cagaagaatt ccgccaagtc agcggaatct attctcgcgt attgtctgga
    31801 acagacgaag caatgcctca gaaaaaagca gcgtattacc ttgcctctgg acaattcgat
    31861 caagtagttg gcgattacta cggaaaaaaa tattttggcg aagctgctaa aaaagacgta
    31921 gaacaaatgg tgaaaaaaat gatcgccgtt tataaaaaac gtctagaaga aaatgactgg
    31981 cttggggatg caacaaaaga aaaagccgtc ataaaattaa ataacttagg tatccaagta
    32041 ggctaccccg ataagatccc agcattattc gaccagttca aaacagtacc tgcagatcag
    32101 ggtggtactt tgctttcaaa tactcttgtt ttcagcaaac tcacattaaa agatcgtttc
    32161 agcaaatgga acaaaccagt ggatcgttca gaatgggaaa tgagtgctga cacggtcaat
    32221 gcttattatc atccatttcg gaatgtgatc gtctttccgg cagcgatcct tcaagcacca
    32281 ttttacagtc tagaacaatc aagctctgct aattatggtg gaatcggagc tgttatcgct
    32341 catgagattt cccatgcatt tgataataac ggcgctttat ttgacgaata tggcaacttg
    32401 aacaactggt ggacagaaga agatcttaca caatttcaaa acaaagcaca agcaatgatc
    32461 gaagaattcg atggtctgcc ttttgctgac ggaaaagtaa acggaaaatt aaccgtttcg
    32521 gaaaatattg ctgatgcagg cggtctaagc tgtgcattgg aagcagcaaa aacggaatca
    32581 gatctttcta tcgaggaatt ctttgtcaat tgggcaacga tttggcgtac aaaagcaaaa
    32641 aaagaatacc aacaactttt actgcaaatc gatgtccatg cccctgctaa actccgagca
    32701 aacgtgcaac caaaaaacct tgacgaattc tatgaaacat ttggtatcac ggaacaagac
    32761 gctatgtatc tagcgccaga aaaaagagtg cgaatctggt aagaggttgt gccagaagtg
    32821 gtcagcttca agaaataaga aggaatttgg gaaaatcgct tctcaaattt ttgtgacatt
    32881 tcggcttatt tttcgaagaa gttacttctg gaataccatg tattagaggt tgtgaaaaag
    32941 ctgcttagtt tcaagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata
    33001 aatttgactt actaccgaga aactggcttt tgaacaccgt ttatcgagag gttgtgaaat
    33061 cagcgtcctg acctgagtat tagagaaaaa aacggaaaaa tagttcctca tattttgccg
    33121 tttgttgagc taataccgag ggtcagctga ttgaacaccg tttagtggag agattgtgaa
    33181 aaagctgtcc tgcatcaagt aataagaaca gccaatcaaa agcagcaagg agaacctcat
    33241 ctataaaaag atagaaggaa gtgagacatc tattggtctc atttccttct atttttactt
    33301 tcctcagtca attgttttac atcataaaag gagaaattcc acttattttt ttcgctgttt
    33361 catgacataa acaactacac cgattaacaa aattcctcca gctaatgcta gcaacgggtt
    33421 attaaattct cccgtttttg ggagtccttg agtgccattt ttttcacttt cttttgctgt
    33481 cctatccgga tgagaaggtt ttccatttgg ttttccattt ggtgattcat ttcctggatt
    33541 ttctctttct ggtttgtcat ttcccggggg cacatcggca gtttctcgca cagtgatcgc
    33601 tttttgctgg gtaacagatt ctgtatgagc agaccatttt tccccatcaa atatttccga
    33661 acgataagtg acttctagcg tatattctcc ttttgaaggt gctgaaaaaa tagcggtatg
    33721 attagaaaat tgagcacttg actgtgtttc aacgatttgg atcttttctg ggaaataacg
    33781 catatcttca aagacaggcg ttccttgatt tggtgtgaaa gttgacacaa aaactgtaaa
    33841 aggcttcagt tcaatgatat ttccttctgc attctccgcc acaccatttg cttcttgtat
    33901 aataatcgga ttattttgtt catttggttt tgtctgattt ggttcttgta cgattgcata
    33961 ctctggatat tccgaagacg gtgaggtttc tccgtcaaaa atagcaccta cagataatgg
    34021 aagaccatcc actctttcct tttctgtttg acgattcccc ggtttaatct gcatcagtcc
    34081 atgaacatgg atacttccat tttcatagcg cgtaacaggc tgctgacctt ctttcgaaca
    34141 tgtatttaga gactcgatcc aatcttttga tatggtatgt ccaagagtat tttctgattg
    34201 tttctctcgt tcattccaat agtagttcaa cgagtggtag atttgatctt cgttttgatt
    34261 caatgggcgg atctgctctc tcatctcaag attctctgtt cggaaagata agataccaga
    34321 agcatggtag tctgtgtttc ctgctgaaga ggtataaaga gccacattat atgaaccgtt
    34381 attaaaactt gtactgctat tgacgataat atctgttccg ctattggaat cgattccttt
    34441 cgcaagattt tcgaaagaca aactattgat caattcatga gggcccgtta aagaagaacc
    34501 gcccattttg aatccattac cgttaccttc tccctcaatt cctggtaccc agccgtttcg
    34561 ataagcaact gagtttttga tgattacttt tccgatcgaa cctgtttcat ttttagcaaa
    34621 aaggtcccaa ccgtcatctg cattatgata cgcgatacaa ccgtcaaata catttccttc
    34681 tcctattgtc agtttggccg caaatccatc ggcatcttca aatcctggat cggcattttc
    34741 aaaagatgta cagttcttga ctaagttatg cgcgggccat gcttcgaaag gatcatttga
    34801 gctaccgctg atttggattc ctgtatttcc attccgataa gcatgaatct cttcgatcaa
    34861 attatgattt ccagaaatct gtaatccttt ttgcatagct gcactatttg taatgtcaaa
    34921 ccctcgtaaa ccccagtagt gactgaacag tgtcatccca tttccagttc ctttaaaatc
    34981 aagtacagga cgcttgtttt ctggattttt ctctgcaatc aactgaatcg ggttttctct
    35041 tgtcccgtta acgccttttg gaatcgtcaa tcctctttcg aaggaataag ttccaggcgc
    35101 catgacgatt gtctgcccag catatgcgta tctcaatgct tgtgtcagag ataatggatt
    35161 ttgtctgctt ccgtttcctg catctgaccc ttctggtgtt acataaactg tcgaatattt
    35221 aggtttacgg tagtccactg tatgaagaat ctcttttgtt tcataactag tcatctctac
    35281 gtattcttct ggcggatagt ttttatctgg cgtaaaactg aaagtaaatt cgttcgttcc
    35341 ttccatcagt tttgtctgat aatcaaattc ccgattcgca gtgatagcca agttttttgc
    35401 gttcttaagc acctctcctg ttgctttatt tttaattgtc aataatccgt cagcatttgc
    35461 tctaaaggac atagtatgat cttggtttcc tgaatgatta ctagaggtca cagtgtaaat
    35521 cggatcaatc aagcgagtag gtctttcttg cccaggagga tctgtcgcag gatctgagta
    35581 agcaacttcg attccttcta ccttgatcgt catattcctt gctgcagcaa atcctgcata
    35641 aataaccgat tcatctgaag cgtataattt ttcccagtca tacatgatgt cagaagccat
    35701 ttcttctcct gtttcaggat cgtaataata tgcctctaaa ccagtattcg tttttttcat
    35761 ctctacgtcc agtttagtaa tcgagttcgt ggccggaaac agattcccat gaccgtttcc
    35821 gaatatatta taagaacctg tttctttttc caagaaccct gctgaagtct ctaaaggtgt
    35881 tgtttccact gagacactcc caggagctgg cgcagcttgc ggatcttcag atgatattcc
    35941 tgtgattgcc cgtgtcccca atccaagtcg catcgtatat ttgctgccgc tcgtgttgac
    36001 cacttcttgt ctatccgagt cccagacata ttcgatccga ctgcctagta tggcaaatga
    36061 atttgaataa aatgcctgcc caaacaaatg attttttggt actgcgtctc ttaccattaa
    36121 agcaaatcct tcttgggcat tcgtataggt ccaagattct atttcgatcg tcgcccgcat
    36181 cctaaagttg atgtctttgg ggattgcttg ataataatag gtcattccat ctgatccgct
    36241 tgactggaat tttccgccgt tattcgtgga agagagccgc acgccgtctt ctaatggttc
    36301 aatggcatac ctactagcac ttgtgctttc tccgaaaaca acccctttcc actcttcatt
    36361 tgcttcttca gattgctttt ttgtcttttc attttctgat tctttcatca taggatcatt
    36421 tccttcttcc gtatccatat cttcactagt tcttggtatt tcctccattg ttgacatccc
    36481 ttcaatgttc acgtgtgcat tttcttcgtt cacagactgt tgttcgttag cagcaacttc
    36541 aacgacagaa attaaaaccg gggtactgag cataaggaga ttcatcatta gcgcactgta
    36601 ttttatacgt ttttttaaca agtgtattcc tcccgtaaca aattagaaag cgtttactta
    36661 aagtattacc taatttattt ttactgtcaa taaaaaacaa aattataata agtgtatttt
    36721 ataaaaagaa aaaatataac ttaagttgtt gataataaag agatttatta acggaaaaag
    36781 aataagtttt tttaaaataa cgtataaaaa agagcataaa acaaagtcgt ttgttttatg
    36841 ctcttctttt attattttgt gctttttatt ttatcaaatc actttgtttt tttataaaat
    36901 ttctgcgcct agtgtggact caaaatgtct caatgcccat tcatgtccaa cgggatcgaa
    36961 acttgccacg gaatctttga aaatatggag tttgtatcct agattatacg catcgactgc
    37021 tgtatgcaac acacagatat ctgtacatac gccggtcaaa taaatatctg tgatctggcg
    37081 ttctcgcaaa cggatgtcta gatctgttcc actaaaagcg gaataatgcc gtttgtctat
    37141 ccaataaaca ttttcctctt gctcatagtc ttggtataaa ctttttaaag agccgtacaa
    37201 ttcccgtcca cccgttccta tcacattatg cggaggaaat aagcggttct ccggatgaaa
    37261 ggaatctttt ggatcatgcg catcaatggc aaaaacgaca aaatcctttt gatcaataaa
    37321 cttctttgta tgagcagtca gctttttttc aattacttgt cctgctgtcc cagtagtcag
    37381 ctttccatcc tctgcaacaa aatcatatgt gtagtcaatc gagatcaatg cgttcatctt
    37441 agtcctcctt atacgagatg tttgacaaat tcgatcacca tttctgctga tcgtttgcct
    37501 gcgtcttcga taaattcgtc aaagctttgt gtcgccgagt gatctgctgt gtcgctcatt
    37561 gcacgtacga tcaaaaatgg gatattgaac tgacgagctg tttgtccaac agcggctcct
    37621 tccatttcgc aagctaatgc ttcagggaaa ttcgttaaga tttctttgat tttatctggc
    37681 gagtcgacaa atgtatcacc tgtaacgatc aatccttctt ttgcgttcaa attcgttgct
    37741 tttgctgctt taaccatttc actgcgcaaa tatgtgcttg cttcataata tagaggcatt
    37801 cctggtaatt gtcctgggtt gtagccaaat cctgttgcat ctgcatcaaa ataagctact
    37861 ttgtctgata taacgatgtc acctacttgt aagccttcac cgattccgcc agctgaacct
    37921 gtattgatca ccatgttcac gccgtattgt tggatcaaca gacttgtggt aatagatgct
    37981 aagacttttc ctataccgga acgtacgacg atcacttcgt ggttacctaa tgaacctgaa
    38041 ataaataaag cgcctgcgcg ttcccatgat aatggttcgc ttaggttctc tctcaaaatt
    38101 tttacttctt cttccattgc gccgataata ccgattttca tctttttaat tccttctttc
    38161 tatataaatg cgattgctaa aaaaaacaca cataacatta ggataacaat aataagagat
    38221 ttcatcaaaa aagagttcca tttggtcgtc ttttcccgtt cgctgatcct tgttttcgtg
    38281 attggtttat tctttttgct ttctttacga taaaagctgg caatcttttt ttcttcttgc
    38341 tgataagctg tttctgcttt tcgctgtttt tttaatgact cgcttgcttg tgcttgttgt
    38401 cgcttgcgaa gttcgcttcg cgtaataagc ggtcctttgg ccataagccg atccctctac
    38461 tttcctttgg tcaatagttc ccagtattga atcgcataaa tcgtctttgc atcacaaatc
    38521 aactgatctt tcatcgcttg ttgcgcctct tcaagagtta gatgatacaa ctccagtact
    38581 tcgtcttcat cttgtgctaa aggattctcg accttctcta ccccttgtgc atgataaata
    38641 tgaaggactt catttgcaaa acctggtgaa agatacatcg aagtaagatg gctcagcgac
    38701 ttcgcacgat agccagtttc ttcttctaat tctctagctg ctgtcatttc gggattttgt
    38761 ccttctcctg gatcgatttt tccagctgga atctccaaaa tcactttttc taaaggtttt
    38821 cgaaattgtt tgacaagaag cagacgatct tgttcgtcaa aagcaatgat cccgacaccg
    38881 ccaggatgaa ataccagttc tctcttcgaa gttcctccat ctggcaaacg aacttcgtct
    38941 acagcaacat caataatttt tccgctatag atttctttgc gactgattgt tttttcttca
    39001 aattgctcat tgttcagcat atcttctcct gctttctttg taatcataac tgcttttttt
    39061 tcatttttca atttttctca aaaactcctt attagtttac catagataaa ccttagattt
    39121 tctctaaata tagaaaatcc ttctttagtc cgatactctt tctagtagtc cgtatgctaa
    39181 aataagtatg taatcgaata acgaaaagaa ttcaatactc aacgataaat gacatggagg
    39241 gtttattatg ataaaacgaa attggccgtg gcttctagca ttggtcttgc tatttctctt
    39301 tgtccgaaac aatagttttt cttttgttct aggactttta ttgataggtg tagttttagt
    39361 tttgtgggga ctctttggtt cgcgcaggaa aaagggaaaa caagaagaaa ttcctgcact
    39421 gactgacgaa cttgaaactc actatgaaaa aagcggtatg acagcgaatg aaatttcatt
    39481 ttttcgacaa acaatgaatc aaacaaaaaa tgagatcaat caattgcaaa tgaatatgca
    39541 gcagacagcc aaacttaaag caatcgatct ccgccatgat ccagtcaaag cagcgaaagc
    39601 tttattcaaa gaattggtga aagaaccaaa ccgactgcat gaagcaagtc agtttcttta
    39661 tacgcatctg ccgaatctag ttgatttgac aaataagtat aatgagatca atgaccatga
    39721 gatcaaaaac aaacagacct atgaaaaact agaagaaagc gcgcagatca tcgaccagct
    39781 gtcagcttta atcgcacaag attatcaaaa attcgttgct gaggatctag atgatatcga
    39841 cgtagagata tctgttgcga aacagagctt aaagcgtgat aataaatatg aaccagaaca
    39901 aaaggattaa ctcttttgtt cataaaagaa gactcggaac attcagatga tgtctggctg
    39961 tttcgagtac tttttctaaa gtagagacat tcatgtaaga atcgagaaca gattcaacaa
    40021 ggaggaattt tttatggatc ataaaccgac tgaaacaaaa gatgttacac cagttaacga
    40081 tacacttgaa gatttattga acaacccgtt ttcaactcca acagatacgc tgaccacttc
    40141 acaacaagcg gagattgatc agctccaaga gcaacagaca gctgctcgtt taattgacaa
    40201 actaccggcg gaacgccaag aacaagctaa acagctagca gcaaaaatcg atgctaatga
    40261 tgcacagtct gtcatcagct acggatcggc tgcccaagca aaattaagtg aattctcaca
    40321 atcgatgttg aatcatgtgc aagcacaaga tattggtcct gtaggagatt ccttaactga
    40381 actgatgtat cgtctccaag aagccaatcc tgacgaactg cgtgccggag aagggaacat
    40441 cttccagcgt gtcttcggga aggtcaaaca atcgatctat gaagtgacag caaaatatca
    40501 aaagattggt gcacaaattg acaagatttc agtgaaatta gataaagaaa aagacggtct
    40561 gctaaaagat aatctgatgt tagaacagct ttaccagaaa aacaaagact actttgatgc
    40621 actgaatatt tatatcgctg ctggagaatt gaaaatggaa gaacttcaaa caacgatcat
    40681 tccagaagcg atgaaaagag cagaagaaac tggcgatcag atggatgttc agattgccaa
    40741 tgattacacg caattcctag atcgtctgga taaacgtacg catgatcttc gcttagctag
    40801 acagatcacg atccagcaag caccacaaat ccgtttgatc caaaacacaa atcaggcctt
    40861 ggctgaaaag atccaagcat ctatcgctac agccattccg ctgtggaaaa accaagtcgt
    40921 gatcgcattg accttgcttc gtcaaaaaga tgcagtaact gctcaacgcc aagtctctga
    40981 aacaacaaac gacttattga agaaaaattc agaaatgctg aaaatctcag ccatcgaaac
    41041 agcgaaagaa aatgaacgtg gaatcgtgga tatcgagacc cttcaaacca ctcaaaatga
    41101 tttgatcgaa acaatccaag agacattgcg tatccaaaaa gaaggaaaag aaaaacgtcg
    41161 tcatgcagaa gtagagcttg gacacatgga agaagatctg aaacaaaaac tgttggattt
    41221 aacccaatag atcacctttc gcttgttact aaaacaaacg cccaacaaca gtttattaca
    41281 actgttgatg ggcgtttttc ttatctttct tctaacttct ccattggagt ctatttctca
    41341 ttcatcttgc tgtgtccata gccatagcca aaataaatga tcagtccaat aaccattacg
    41401 ataccgaaca cgatccatgt cacagtttgc agacgaagca tcaatacaat acaagtcaag
    41461 atggagacga ttggcaatag cggaacgaat ggcactttga attctccagc ttttggtttg
    41521 ccaaacattt ttctcaactt cagtaagcct agactaacaa gcatcaaata ggcgagcgcc
    41581 atgatattcg ttagttccgc taatacattc aatgggaaaa atccggcaaa gaatacagct
    41641 gccacaccag cggcataagt cgcttttttc ggtgtacgat gcttttgatc gatttctgtc
    41701 ataaacttag gaagtaagcc atccttgctg acagcaaaca gtaaacgagc aagtgaatac
    41761 atcatcgaga tcgttaccgt cagtagcgtt aaaattgccc ctacagcgat caccgcgccc
    41821 actgttccat gtcctacata cctcattgca aaagccactg ggtcattgac tcctagttca
    41881 gtatacggaa cgattcctgt caagatcagc gtcacgatga tgtaaaggac agtagcaatc
    41941 agtatcgaac caatgattcc tcttggcaca tctttttgcg ggtttttgac ttcctcggct
    42001 gccattgcaa cggcatcaaa tcccaagaaa gcaaaaaaca caagagctgc accatctagc
    42061 acaccggaaa aaccaaaagg catgaatggc tgccagttat caggtttaac gaaaaatatt
    42121 cctacaatga taaacaacgc gatgatacca aatttgacat ataccatggc attattcaaa
    42181 cgtaatgctt tcttggcttc aagcgacacg atgtaagtga cgaatatcac tacgagcacc
    42241 gcaatcaagt caatgtatgt accattttcc ggattgtacc cgccagttaa tgcttgtgga
    42301 agttcggccc ctaaactttt caaaaaacct tgcatatagc cagaccatcc ggaagcaaca
    42361 gaggaaaccg ctagtaaaaa ttccccgatc aacaaccacc ctgtcagcca ggcagcaaat
    42421 tcaccaaata caacatacag ataggcatat ggcccaccaa tcactggcac tcgcgaagca
    42481 aattctgcga aacttagtcc agacaaaatg acaacgattg cagctaagac aaatgaaagt
    42541 gacagtgctg gaccagcatt ttgattggcc gcaacccctg tcaccacaaa tattcccgta
    42601 ccgatgatcg caccaattcc aagcatgatc aaatcagacg tttttagttc ttttttcatc
    42661 tcactcgtat gattgagatt gatctctttc ttacgaaaca aatccattga agttcctccc
    42721 cattttaggt tggaaaacag cgattaaact gttttcatat accctaaaag gataccacct
    42781 aaaatgacta aaaggcaacc aacaataaca taaatgagct cttttttcgt ttttctttcg
    42841 ccaaggatga aaatcccacc taaagtagag ataatgatcc ccatttgtga aagtgagaag
    42901 ccaacagcta aaccaagtga tttgactgtt agaagcatgc agatgtttcc taagccccat
    42961 aataatccac agatcatgtt tttccacaca aactgatcga ctttaacttt acggaaagca
    43021 aagaagcttg cgcctaagat cattccgata ctttgaggta agataattga catcgcatcc
    43081 aaattcgccc agttgacgat gatggtatat acaccataac caatcgtaga gaagatcaat
    43141 gctctaaaac ccttaccaaa atcaagcatt ttattatctg tatctatttt tccttcgtca
    43201 tcttgtcggg cagtcaaata cgcaccgctt acaagaagag ctaaagcaat aataccgtat
    43261 acataatctt tcgtttgtgt ccattcatgg aagaaaacgg ctcccgcaat cgtattcaaa
    43321 attaattgaa aaccagtaga caatggtagc cctacagaaa cacccatgta cttcattcca
    43381 tggaactgtc cattttgtcc aacactccaa gctaagccgg ataaaaatcc gatcaagaaa
    43441 atccaagggg tgattacagg actaacaacg aaaaagacaa ctaaagaaaa cagaaatgca
    43501 ccgatcgtca ttcctagtgt ttgttgattg gcatcgccac caattttacc acttactaaa
    43561 ccaatactac cccatgccaa catagggact aatgctatta aaatttccat aaccaatctc
    43621 cttttatttc acatgataag aaacttatca gcgaaaacgt taacaacaca agcacttttt
    43681 ttcgtttcta acgattttct aacgtttttc attataaaaa tttaagattg ggaaaaataa
    43741 agtcattgtt gtcccttttc cttcagttga ttcagccgtc aaatggatac ctaatttttc
    43801 ggccagattt tttgctaaat aaagtcctaa tcctgttgaa tgtgttttgc taagtcgacc
    43861 atttctccca gtaaatccct tatcaaagat ccgtttcaag tcttccgctg ggataccgat
    43921 ccctgaatct ttaactgaaa ggctgactcc ttgcgcttcc ttttctatgc tcacaatgat
    43981 gtttccgtga tccggcgtat atttgatggc attactaagc aattggttga agataaaagc
    44041 gatccattta gcatcggtca aaacagtatg atcctcacca acgatctcgt agcgaatatt
    44101 tttttggata aaataatttg cctgactgcg tatcacaggc tgaatgattc cttttagtcc
    44161 atattcttgg atgaggtagt cccgcgagaa actgtcgagt cttgcataat acaaaacttg
    44221 ttctacgtat tcatcgattt ttgataattc attttctagt aatgtatact tttgatcgtc
    44281 aatatcgaac tcgatcgaac gaactagcaa tttagaagcg gaaagtggca ctttgatttc
    44341 atggacccat gagtcaatat aatctttttg gtcttgttga ttactgattg ctgattgcat
    44401 tacttcttgg tgttcacgga tcagctgatt gatatattct tctaccagtt tttcttcttc
    44461 tgtcctccca ccactcaaat aattttgcaa aggggaagtt ttttgtatgt cggctaattt
    44521 tccccaccat cttctcttgc tcaagtaacc acccagcaga taaaaaatca gaaagacgcc
    44581 ctcaatcaaa gctaaataag caattgtgct caaatcaact ttgatgtttg gcgccagcca
    44641 catcacaaag atcgtcaatg caataaaaaa cagccatccc aagatcaatg tccactgatc
    44701 tttcaaatat ttaaaaaaat tcatcttaac cgctcatcct ttcagggaac gatatatccc
    44761 tgtcctactt ttgtttcgat atatccttgg atacctgcct gttcgatttt tcggcgtaac
    44821 cgattgatgt tgactgtcaa tgtattgtca tctacaaatc gttcgtcatc ccacagtgct
    44881 cgcagcaatt tttcccgact taagatcttt ccattgtgct tcatcaaaat aaataataga
    44941 cgatattcat ttttgctcaa atcaatgatc tctccgttga tttccatact tccgttgtca
    45001 acattcaacg taatgccatt gtggctcatc atttcactag ataattctgt gtagttgtaa
    45061 gaacggcgaa gtaatgcatt gatttttgca atcagtacat cgatttggaa atgttttgtc
    45121 acaaaatcat ctgcccccat gttcatagcc atgatcatat ccatattcgt attcctgctg
    45181 gagataaaaa tgatcggtac tttcgatact tcacgtattt tttgacacca ataatagcca
    45241 tccaaaacgg gaagattaat atctagtaat acaagctgtg gttcttctct ttcaaagtct
    45301 gagaacactt gattgaaatc ggtcgttcca aaggtttcaa attgccattt ctcaagttcc
    45361 tccatgatca attgtcgaat gatctcttcg tcttcgacta ccatgatttt tgccataaaa
    45421 aatccctcca tctttcttct tgctactaca atatcaaaga agcccttgtg aaacaagttg
    45481 gcttacctgg aaaatgtcat tttagcaaac gttaaaaaaa actggaaata tgcgatttaa
    45541 tcgcgtttgt ctcttccaag cagcttaaat ttattatgtt attcttaata attggtctat
    45601 tatctgttat ttttctttcg ttgtttttgt cttttaaatt ttcgataaca ccaataaccg
    45661 aaaagcgtta gcactagcaa tatacctaga aggagtataa gtaatcgata aagatggtat
    45721 ttttttgtct gttctatctt tgaagagact tcttctgtaa agggaactcg tcttcctgtc
    45781 actagtaatc ggtgagtgtt cacggtgtaa ggtgtacaag tgataagtgt gactagatcc
    45841 tgcttttctc taattgctaa tgtatttgtt tttgttggaa ggacaacaat tttctcaacg
    45901 acttcataag cgagcatccg accatacact tcaagataga atcggtcatc ttgctccatt
    45961 tcttcaagat ccgtaaagag ttttttctca gcaataccgc tatgggcggt aatcacggaa
    46021 tgtgtactct ctcctccagt tggaaaagag gtgccttgca ggactgtcgc tcctctatct
    46081 aacaaaagat cattcgtttc atcaaataaa ggcaagctaa cattgatttt cggaatatag
    46141 atggctccga tcatatgttg ttcgtaatat tcttttcctg gattttggac atttctaacc
    46201 gcttgctcaa aaggatcttt gacttgtccc attcctggga ttactgatgt atgagcaagc
    46261 gtctgatttt tcttttcatg cactgctaat tgttgctcat gtttttcttc tttatcgcgt
    46321 tcaagctgct tttgataatt ctcgatcctt ctttgatcca aataattgct gagagcatct
    46381 gctaaaaatg gatatgtaaa aactgcagct ccggcaagaa acatcactgc cataaataat
    46441 tgtaataaaa gactgatttg ggtttgaggt ctattaccta ttttcttttt cattttcttc
    46501 accttcttat ttaaaaaaac tgcagcaacc tttataaaac cgattgctgc agcttatttt
    46561 tgttgcttat acttcagact tcattttaga tttacggtac cagctgtacg ctccaatcat
    46621 caaacttatt ccgattagta agaatgccaa gagcccgttc ccaccagtag caggaagtga
    46681 cccgcgtttc atattcggta ctctttctgg ccctggattt tctactccta cgatctgaat
    46741 ttcttcatta tagcttccat aagagatgat aaattcagta aaggctgcct cacctgttgg
    46801 aagtacataa ccttctggcg ccatggtctc tttcatttga taggttccat aatctaaccc
    46861 ttgaacctca aagtatccat ttccatctgc gacaaattct gttgcttgtc cgcttgttgc
    46921 ccactcaatt ctatctgctc ctgattcata aacagctaaa ggcgactccc ctttataaaa
    46981 tattgcgtat tcagaacggt cttcattcca caattcgaat cttgcatctt taagcaattc
    47041 actgcttgat gcatcatatt tttcaaactt tcttccacct gttgtgactt tctctggcgg
    47101 ctctactcct tcttcataat caaattcatc atttcgtccg atttcaataa ccgctgtgtt
    47161 gttatggaaa tcatccgctt ctagttctgt ggaaaccttc aaattatacg tgatcgttaa
    47221 atctcctcca cgtgcatctg ctaatctacc taaagtatct actttggcta ctccattttc
    47281 ttctgtcaaa atcgtcacga caaacccgtt gcttactact tctactatat aatcttgtcc
    47341 ttttgttaaa atcgtctcgc cggctcgcac ttctatttcc tgatcataat aagttagacc
    47401 aactgtaggc gtatctgtga ttttgtaatt tttgataaca gcgcttccgt tttcaaggac
    47461 agaatcgatg aaataaggaa taggtgctgt gatcgtgtaa gaagccgttt ttcctctttc
    47521 tagatcaata taagaaatcg tttcttgttc tccagcttca ttaataatcg ttacttgtct
    47581 aaggtcgctt tcctctacat tcatctgttt atctgcttct ctgatttcgt ttttcggata
    47641 caaatgcaca tctgtattgt atgtgtcttt ccatgtagaa ccatcccaca cgccatccac
    47701 catatcaggc atcatcactg gcatcataac aaccattgga gacgcgattc tggaaatatg
    47761 agctggcgaa taagtttcta agaaaagata gactttatct ctagaagaag gtgcttcttc
    47821 cgtcacttgg acggtagaga ataccgcttc tccagcttca ttcgttgttt gtttgtcaat
    47881 acttgtcagt cgatatttta aaaaccaact ttctgtgtgc cgtttttgaa tccattcaat
    47941 cgcttcggca gaattaagac cgtcttctcg ttccggtgtt aaaggattat ctcgaatcaa
    48001 atcgtaatat acttccgtta cgtcaaacaa atcaaaatcg actcctggaa tattttcttc
    48061 accaaaatcc gtagaaacca atccgctatt cggtcgttct tccggaacag atgagaaacc
    48121 ttttttgtgt aatgtaatcg tcacttcact tggtttttgt gcgacttctt cggctttgac
    48181 tagttttccg ttcccgaaaa tccctattcc cgcagtaacg agtaacagta cactcattcc
    48241 cgtgacaatc atttttttat tcattttttt ctccccgttc tttttttatt aataaaacag
    48301 cacctaacaa taaaagaatt cctagtaaag aaatcaacgt tcttgcttca ttcgtttgcg
    48361 ggaatacgga ccattttcct gattgacctg gattgcttgg accactcggc tctttcggtt
    48421 tgtccgttgt ttcttttacg gtctcgttat aaacacgtgg tgttgcggat tcataggcag
    48481 agactggaac tttcccggat tctaattcag ccatctcctg tccgttaact actacatatt
    48541 gtggttggcc ttttgcatcc acccaaaatt cagggatgat gacctcgatt ctcttacttg
    48601 cttcatccat ctcgtaacct tccacacctt ttaattcttc aaaataatag gtgccagaag
    48661 gcaagaaacg ttgtcctgtc gtaacaaggc cctctttatc cgaaatgaat ttgcttacat
    48721 ttttgtcatt caaaggatca ttgtctgctg gtttgatcca ttgatttttc aagtcattag
    48781 caggagccat atctaaataa tatttccccc cttctatcat ctgatataga gcaaaaacag
    48841 caccttctaa tggttttcct ttttcagttg ttcctctttg acgtccatac ttgaagaagt
    48901 atggatctct tagataaccg acatttttag gatagatatg aatctcttgc agttcctcgg
    48961 ttgattctgt cgggtgataa attggcaaaa tagcagcaat tgggatagct gtctgatcga
    49021 tatcaacatc caatccaact tctggatcaa tcgattcttc gaaaatcaaa taaactgcat
    49081 cccgatcttg gtttttctta ggtgctttga tacgtgcaat cccgtctcct aatccgccaa
    49141 ccgtatcttt cccagttgtt accgaagtcc ctatttggac aagcctttct tctgtgatta
    49201 gctgacggat attgtttctg ttcgtttcag cgatttgctg gacgaattct ttaggagtca
    49261 tgttttcagc agcttttttt tcatgataat aatcggtcat gtcgtaaatg gcaaatgtcg
    49321 ctccatttag tggcaccgtc tgtgaaatga tatctgctgt ttcccctgct tcctcagaga
    49381 tagccaagcc atcattttca tggtattcaa attgatcgtt ataatcgata tctcgaatca
    49441 tccgtttatg caacacgaat tccacgacat cttcccctga ttcttctgcc gacatcgcat
    49501 gtggaagaag gactgctgca agcggtagta aaacaagtaa ttgaagcatc aaacacaaca
    49561 gctttttcat taccttgctc cctccttttt agattgtctg acaaacaaac cataggctgc
    49621 tcctgcaagc gccatgaaga atgcagcaag caggatatag aataatcgtc cggatcctcc
    49681 tgtagcaggc aacggatttt caggatcgtt cccgacacta agctgtaatc tgattcgatt
    49741 tgtctccaaa agttcatatt ctaactcttg ctgtagatca ggcgtctctg cttcttcgtt
    49801 tggataatac gaaatctcaa cttttagtgt acccgcttct tttcctggaa ctacccgatc
    49861 attttctatg tcaggttctt ctcgataact aatttctaaa ataaagtaac ctggaagttt
    49921 tctatagctg tcaggtgact tcacttcctt gatccgatag accccttcca acaagtctga
    49981 agcattgaat agggaagaat cgtttggatc tggtgccaag cctccctcga tgatttcata
    50041 ctctcctgca ctattttgtt tctcaattga aaattctgcg ccttcaagac gtcggctatt
    50101 catactgtca attttcaaaa gttccaaatc gattggcgct agttcatctt ctaaggttac
    50161 ttgtagtaca ccatcgatga tttcatagcc ttccggcaag ggagcagttt cgtcttcgat
    50221 gaccgtatct tcaatgataa acttaccttc ttcattcagt gcaaactgga acacttgatc
    50281 agatagccga tacccatttg gtgcgacaga ttccgagacc tcatattcac caatagacag
    50341 atgcagaggt tggtcgtaat ttaaatcgac atacttttct actcctacgg cacgagaata
    50401 gaagtatccg atcccaggca aacgtttcga ttcttctggt tctaacggat taccagtgta
    50461 tgctcgatac agcgggaagg aagaatcccc cactttcctg atatcgaaac ctgcaccatc
    50521 taatgtatgg ccttcatggt ctaattttct aactcggatc tcccgataat caaacgtatt
    50581 gtaagcagtg aatgctaccc ataaacgatc ctcttcatct gttgtatacg tgagatattg
    50641 ccaattttct tcacctgttt gcttgagttg gatagtccct atagcattat cgtaaagtcg
    50701 caagatccat gtgtcatttt catcaggttc tttgaaacct tctgggttct ggctctcaat
    50761 gattttgtat tctgcatagt tggtagtttc ttcactatcc ttataaacaa atgaatctgg
    50821 ctctccgatt ggatgaataa ctttctcacc atccacgatt acatatttcc cgtcttcatc
    50881 tactgaatag aacgtgagtc tccctttctc ttcctctact tcgttcacac cagaaagccc
    50941 tttcgctact ggatatccga tttccagtcc gttaccatct gtttcataaa gttcaaactc
    51001 tgcgccattg attggcatat tactgccttc aattagttta ttgatattca tgtggattgg
    51061 cttgaattca tttcgcattt ccagtttgac atacaactca tcatactctg tgccttctat
    51121 gttaggctct tgtacaacag aaagcttgtg atattctcct tcataatagg aattcggatc
    51181 tttaccttcc ggtccgccga ctctcgaacc agatgtatat tcatctacaa gctcgtagtg
    51241 acctaacatc tcatcttcgt tggctgcata ttttttgatt tggaattgat acgtctcatc
    51301 tactaactga taacgtggtg gtccagcgat ctctctgatc caaaatatgt cgctgactgc
    51361 tccagtttca tctcctaaaa tcggcatctt cgtttcaaag atcgctattc cgtccgtggt
    51421 agttttactt gtttcgattg gttcggcttc cccactctct ggattttggt catataatgc
    51481 aaattcgact ccagccaacg gctcgccatt ttgacccttc ttgctgactt ggaaattacg
    51541 gcctttatgt tcgtaacgaa tggaagaaga tgggaaatac atccgtccag tagtgatatc
    51601 attctcataa tcctccaggt tctgaatata ggccatattg ttcactgtgc aaaaatttcc
    51661 gtcttgagct cccgcatcaa gttttacttg gtacttgatc ctgaccattt ccccttcacc
    51721 taaagaaatc ggagaaaatt tataagcttg gttttcccgt ccgacatacg ttgtcggagt
    51781 cgccactact tctggtgggg cttgtccatg acgttcttca tcccatattt ctggctcttg
    51841 gtacttcatg ccgttttctc ctggatctag atggtaaaac tgaatatctg ccgatctgcc
    51901 agctccaaat ggtgagacat taaaaccagt ttcatcataa agtgtggctt gctggatcgt
    51961 tctcttgaag gtagaagaga tatcctccaa ggctgtaaca atgtcattct tgttgttcgc
    52021 attgtaatat cgcggtgtta cgccatcttc ttctaaatca gcaatatttt tcaatacatt
    52081 tcgtccaata gcatctagtc gttgtctccc gctaacgttt ccatcgattc ctaaaccaat
    52141 ggagaagata cttggcgggt tctcttgata cttgtttttc agccaatgat gataagcaac
    52201 tgtgttgatc gcagatgact tttcttgcga agtaggactg ccattcgtat tgttattaga
    52261 agaaccatca ccaacccagt gagtcgcttt attatctcct tcttttacct ctccataacg
    52321 atagtgtaca tttacttcat cttctggacg gttaaagtca gatgcatagg taactggatg
    52381 agtaaaccct gcattagaag tattcccaga aaactcctca aaatttctga acaaaacgtc
    52441 attatcttca gcaatcttat cttccatcac agtccagttg ctccaagctc cattagtgct
    52501 cgtacgataa cttgactgaa tcggtgcata actgaaggtc ggtgtcccat ctcccacaac
    52561 gattaggatt ttttctggat ttctattttc tccgccattg tctttataca atgtttcata
    52621 gccattttta agccctagtg taagcggagt tcctccgatt ggtgtacgat ttaatgctgt
    52681 attctcactt cccgtcaaac gattgatatc atttgtcaat ggaaagatgt ccgaactgat
    52741 ttgctcatgg ttattgatag ctgtactatt atggtaaaaa ttcaccatac cgattcgaat
    52801 atccagactc gggtcagaga gcagatcttt actcatttcg tttactgcct caatcaacgc
    52861 agcattttta gtttgactat tggtcccagc ggttaattca ctcatactcg ctgacttatc
    52921 tagaacaaaa acgatatcga ttggactgat ttcctttttc tcgcccccga tgacatctag
    52981 atagacattg taaagggttg ttgcatcttc aggatgtaga tcagatgctg tgctgcttgg
    53041 cagaacaggt tctacccatt ttttgatgta cgccccgtta taatccaagt aactgttttc
    53101 aaaattccgt tcatttcccc aaagctgaac ggtttgagct ggaacgtttc tatcttcttc
    53161 gcctgcagtt gatccattat tagtagcata gtcgtaattt cgccagttcg ctcccccgtt
    53221 tgtataatca ctatcatcga tcaagttctc tccgtcatat ggcgagcctt tgtctccttg
    53281 atagcgttca ttgctttgtg tccaatttcc taacactttt gtatagtcat tcgttgtaaa
    53341 cttcgggtag cgatgctgtt cgttttcaac ttctccgcta ggatagatat attcataggg
    53401 gtcttgcgta gctgttggcc caagacgctc caaaataccg ttgaataact ggcgttgaat
    53461 gcgctggagt gcttgaccgc tgggtacgat tggttcgtcc agcttttcca tctcattttc
    53521 ttcgctacgt tgaatttcag cggaatcttc tgtatcagtt tcattttctt gtaattcttg
    53581 gttatctatt ctttcatcaa ccaccacttg aagaggtagt tacccttcgt tcacatcggg
    53641 gatcttttct gtttcaaaaa tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc
    53701 gacataccat tcttgaccat cgatcgtctc gacttttagg tcttctccat cttcatcata
    53761 ggaggataga cctgttccac gaacgttttt tacggtacca atcccggcgg ctgccgcatc
    53821 gattgctaaa cgtattctac cttcattggc actttccttt ttttcaaaag ccagatgcca
    53881 ttcaagggtt tcttcttttt caatatactt cccttcaatt tttgcatagt cctcatctaa
    53941 gaatacctca tcctcctttg tttctgttga ctcagcagct acatccaggg tcgtcatatg
    54001 atagactgac tggaaatatg gtccaaatag caaactgaca gttactaact gcaaaagctt
    54061 caattttaac cctttcacat ccttatactc ctttcaaaaa tatttgtctt ctttcacaac
    54121 aatcaacaaa acaatgccca tctaattata atttaattta attttaattt taaatcaaat
    54181 gatatccttt ttagttaaaa aacaccaatt atacaacatt tattattaaa attaaattat
    54241 aaaattttat aacgttctca tttaacaaaa cttaagttat taagacaaat gaataaacaa
    54301 ctacgctttc ctcgaaacaa aaaaataaag cacaaatgcg catttacgca ctcatgcttt
    54361 attcgtacaa ttaattcaga taacggcagc gcataggatg ccaataacac ttcctaaaat
    54421 agctcctcct aaaatgtcag taggataatg taaaagtaaa aaaatcctcg aataagcgat
    54481 caaacaagca aataaaaagg ctaatattcc tgcaactgga cttaattcga acaacgcagc
    54541 tactgataaa aaggctagcg ctgtatgtcc tgaaggaaaa gaagaagtct tcggtttact
    54601 tgtgtaaagg acaagccgac gatttttttc ataaggtctt ttccgtcttg tcgttttttt
    54661 gataaaaagc aaaccaacaa aaaaatagat caaactgccg ataagaatgg ctagaccata
    54721 ttctctagta tcaggtatca agactaaaac agcagcaagg agcaaccaaa taaaaccacc
    54781 attagagata ttactgataa atttcaaaaa attgattttt cgactatccg ttttccgttg
    54841 cttggcgaac ttgttgataa tactatcatc gattttagca atcaaacgca acatgttatt
    54901 cctccttttt aagaaccttt tcttgcgtac gatggataaa aagcaggact tgaaaataaa
    54961 acaagaaact gaaacctaat aaagcaaaat agattggtcc tgcaaaccag aatgcgacaa
    55021 gaaggatcaa cgtgcctgcc aaatagaaaa gcgcgctttt gaaatcatta aaaaacaaga
    55081 aaaatgcagt aatccaagaa gcttcttttt gttccagttg aagcaagatt ttgtaactaa
    55141 agagaagcgc gcttaataga agcaaaaaca acgtagtata catcaatgta tagccaattc
    55201 ccccacccca atagcggaaa atctgataat cgatcaatag aagaagaaaa taaacacttg
    55261 ctaaactatc ttgctttcca taaagagaga agttctcacg aaactttttc cagaatagtg
    55321 aaccgagctt tttctcttca tttttttgta atgataaatc atatacagaa ctgttcgact
    55381 tgcagttcct aatgtgaaaa caggcaagac gcacaggaac catagtagct gtaaatggat
    55441 aagacgtgga atccattcaa tgatttctaa gaaataatca ttggcttttt taccaatcga
    55501 tcgattcatc atccttttga acctcctgtc aaatccatcc cttcaatgaa ataacgttgt
    55561 gcaaaaaaga acagcaaaat cgtcgggatc aagacaagtg ttgctcctgc catcagataa
    55621 ttccattgtg tcgttgcttg gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt
    55681 tcttgatctt ggatatataa cagaggacct aagaaatcat tccaagctgc gttgaaggaa
    55741 ttgatcgcaa tggtaatcaa ggcaggtttc gtcaatggaa gcatcaaatg actccaaata
    55801 tacaaatgat tcgctccgtc gatttcagca gcttcgatca attcattatt gatcgataag
    55861 taaaattgac gcatcagaaa tatattgaac gcattgccga agaacgatgg aacgatcagg
    55921 ggcaaataag tacctaccca gccgatcttg gagaataata catattgagg gatcatcgtt
    55981 acaaaccccg ggatcatcat cgtagaaaga accaaagcaa acatcagctt tttcccagga
    56041 aaatcaagct tcgcaaatcc ataagcaata aaagaatttg aaaggacatt accgatcact
    56101 actagcacag tgatgaataa tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc
    56161 gtatccagat agttttccca tacgattttt tctgggaaaa aggtcggagg ataacgaacg
    56221 atttcttcca ttggtttcaa tgaagtaaac accatccaaa gcaatggaat gatcaaaatg
    56281 attcctatta ctgtcatgag gacataactt agtatttttt tgtttgccga ttcacactgg
    56341 tctttttgaa ataatccatc atttgccgtc acctccagaa taatagaccc acttaggtgc
    56401 aagcttcaaa ttgacaaacg tcagaaccaa cacgatcaga aacaggatca ttgacatagc
    56461 catcgcataa cccatatcaa aagctacgaa cgccttgttc cacatatgca gattataaaa
    56521 aagcaaagaa tttgcaggtc cgcctgttcc atttttcgtc atgacataag cttcttgaaa
    56581 aatttggaaa gaaccgatcg tagaggtgat cacatcgtaa aaaataatag gagtgattaa
    56641 tggcaaagta atacttttga actttcccca aacgcctgct ccatcgattt ctgctgattc
    56701 atataaagat ctaggaacat tttgtaatgt agctaagtaa agtagcattg ctcctccaga
    56761 actccatatt ttcatcaaaa tcaacgcagg tttcgtccat tgtggatcaa acagccaagc
    56821 tggtccaggg atatggaacc agcccagaaa tgtgttgatc aatcctgcag atggagaaag
    56881 cagctgcatc cataagatgt agactgctac tcctgataag actgccggta aatagaaagc
    56941 tgtacggaat acactgattc ctttgatttt ttgattgagt actgtggcta aaaacacact
    57001 gaaaatcgtc gtcagcggta cattgaagat gacgaaataa acggtgttgt ataacgattt
    57061 ccaaaataaa tcatcgtgaa cgaacattct tttgaaattt gctagaccaa tgaaattcat
    57121 cttggatgtc aaattataat cggtgaaact accaataact gaaaaaagca taggccctag
    57181 cataaatacc aagaacccga taataaaagg cgagataaat aaataggccc atcttgcttc
    57241 ttttttcttt cggatagata aacgcttcaa attttcttgc ctcctctgta cctagacagt
    57301 caaaaatgga ttattgattt tgcttgacta agttttctac tgctttttgt gcatctgcca
    57361 atccttcttt tggtgatttt gttcctaaaa gaatctggtc aatctgttca ttcactaatg
    57421 tcgtgtaatc cggagcatct aaaggaactg gtgtgatcac cgtattttta aagttttcgt
    57481 ccgccatttg atagatcatt tttcctttat ccgttaggtt gtcattattc actaaattct
    57541 cattggctgt cttgctcgcc atgatatcaa aacttttttc gccgaatttt tcttgtactt
    57601 ctggagaaga aagatatttg atgaattcat aagaggcctc aggatctttt gctccgtatg
    57661 gtacttccaa gacgaatccg ccaccccacg accaatggcc tgaaccttct tctttttctg
    57721 ggagctgcat cacgccaaaa tcaaaatcat ccggtgcatt ttccatcaaa ctagagtaat
    57781 aattgatatt ttgggcacgc atggctacta atccggaaat aaatggatca gatacaccag
    57841 acccaaattc ggcttctaat ttattgatcg tatttttccc ataataatct tgccagtctt
    57901 ggatccactc caaggcttcc actttatttt tagtatcaat cttgactttt tcatctttgt
    57961 caaaccaact agtcccgtta tctgcgttta gcgcccatac atcagcccct aaattccata
    58021 atggataaaa accgatgcgt gtaaattctt ttccttcttt tttatctagt ttacgtgctg
    58081 tttcttctaa ttcatcccaa gttgtcggta gatcttgttc actgattcca gcttctttaa
    58141 atatcttttt attataaaaa atgacttgtg tatctgtatt gaatgggatc gcataaggat
    58201 caccttcata ttctactgta tcccaaagct gcggataaaa atccttagaa gtgtcttttt
    58261 ctacatattt ggagagatta gttgcttgct gtgcttctgc tctttgggca acggaattta
    58321 tgtcctgtac aataacatcg ggtgcatttc cagctgttat tgcagataaa gatttagtcc
    58381 agatgtcacc ccatggctga tacttataat ttacatggat ctcgttttgt gattgattga
    58441 aatcctcaat gatttcttcg atagcttctt ttcgtgcacc ggatccccaa aaggaccaga
    58501 aatcaatatt ttttcttcca tcacttgttt tttcagaaga atttgctgca cttgaactgg
    58561 catcgcttgc attacttgat ccgcatcctc ctaataaaaa caatgctgaa cttgccagca
    58621 accctgtaaa caataatttt ttcatatttt acctccaaat tttaatgtaa ccgatgccgg
    58681 ttttaatata acgatatcat tttgttctga aaataacaat ttataaggta tgattctaaa
    58741 aaaattatta atgataccaa tactttatat aattcgcgag cgctttcttt gaatttttta
    58801 atctttcgta gcagaatgag aatgtattaa aaatagatta ggaggaattt tgatgtttag
    58861 acaccagaaa gaattgcagt ttgaagcaaa accagatcgt ccgaatccat taatcgcgaa
    58921 atatctgcag gagctgatcg gcggccaata tggcgagatg agtgtagcta tgcagtacct
    58981 gttccaaggc tggagctgcc gaggagaaga aaaatataaa gatatgctga tggacattgc
    59041 aacagaagaa atcggccacg tggaaatgtt ggcgacaatg gttgctcgtt tattagaggg
    59101 tgcgcctgtt gaagatcaag aagcagcgat gcaaaaagat ccaacaatcg gtgccattat
    59161 ctctggaatg aatccacagc acgcgatcgt agctggctta ggacctcgtc ctgctgacag
    59221 cgtgggcaat ccatggagcg gtgcctatat cattgcgagt ggcaacttgt tagcagactt
    59281 tagagctaat ttaaatgccg aatcacaagg aagacttcaa gtagcacgta tttacgaaat
    59341 gatcgatgat cgtggcgtaa aagatatgct gtctgttctc cttgcaagag atagctatca
    59401 tcaaaatcta tgggaatgtg cagtgaaaga attagaagaa aaagaaggtt caatcttagt
    59461 cccaagtact ttcccgcgtg acaaagaacg aaaagacatt gcttatgact tctataactt
    59521 ctcagaggga gaccaaagta gtgaaggttc ttgggctagt ggcaaagcac tggatggcga
    59581 aggcgaatat caatggttga aagaacctaa gatcgaaggc gctgcaccaa aattagaacc
    59641 agtaacacca aaaatgtatg ggacacctcc attcaagaaa taaatatcta cccgtagaaa
    59701 cacccgtttt ttgttccaca cgctttgtga gcaaaactga ctaaagtcat taatcataac
    59761 cacccttcaa aagggtggtt ttctctagag ctataagctc aaatcaccgg ccagcgccta
    59821 aagacgctgg ctttcatatt ttatgttcaa gccatattgc ctttgccagt tttgcccacc
    59881 tcttaaaagg tttatgtact actccttagc ccttaaaagg atcgtcttac tctcgtacac
    59941 tcagcttatc taacgcacta tcatgttttt cttgttcttg aatgtacttt ttgatggtcg
    60001 cttcattttg tcctactttt gtcacatagt atccttccac ccaaaagtgt cgatacccaa
    60061 atttatattt tagatttgca caatctcttg atgatctcct gaatattggc tcgatattga
    60121 ttataaatta tttttcttct atactttggt gtgaatacaa tgtggtattt acacatctac
    60181 tttgtacggg ctaaactatt tccctttatt gctccacccg catagcaagt ggttttgtat
    60241 ttcaatcgct cggcgcgctc aattaggtct aaagacaata aatctaaaaa cccgctgctt
    60301 cactgacaga aacaacgggt tttcagaaaa aaatgaaatg aaaaagatct cttacggcaa
    60361 tctattttat ttttttgatt ttttataaat ggaaaatgct tcaagaagtc caagaaataa
    60421 agccgtataa aaacaagaac ctatgaaatc tttatttatt agatattcaa cagaaatccc
    60481 gataacacta gccaacaata tatttatcag aagtgtaaaa taaggatgct ttttatagaa
    60541 tagaactagt gttgttttca tagactctcc tttcttcata tttccaatta atcacaaatt
    60601 cacggcatta ctagaaaaat taaaggggcg cagaagtgga aaaatactct tcgactttcc
    60661 ctacaaacca ggccatcttg tgtttaataa ctataccaaa tctaaggtac ctctaaatct
    60721 aaattccatt tgtggcggtg acctccaatt tttaactctt ctatctccca tccagtatac
    60781 ataaccttga tacttcgacc acttatatat cgaacacaca aatacctatc cattgcagga
    60841 gtactaccta acttagttgc tacttgcatc taaataacag ctgaaaagat aggacttact
    60901 cttagtaaag tatctgaact attaattttt aaactatcat ttttatcctc aactacatgc
    60961 acatctgttg caacttcact ttctgtctca tccagataaa tgattaagga atcgggaatt
    61021 gcaatatctt cagctgacgc tggaatagtt gagctaaatt agtcatcata tctttattat
    61081 aaaataac
//
LOCUS       DASNNU010000005        58491 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.005, whole genome shotgun sequence.
ACCESSION   DASNNU010000005 DASNNU010000000
VERSION     DASNNU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.005"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            122..640
                     /locus_tag="U8116_000283"
     CDS             122..640
                     /locus_tag="U8116_000283"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN8433305.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            768..1451
                     /locus_tag="U8116_000284"
     CDS             768..1451
                     /locus_tag="U8116_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEN8433306.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1760..1833
                     /locus_tag="U8116_000285"
     tRNA            1760..1833
                     /locus_tag="U8116_000285"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Arg,seq:tct)
     gene            1992..2351
                     /locus_tag="U8116_000286"
     CDS             1992..2351
                     /locus_tag="U8116_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEN8433307.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2474..4105
                     /locus_tag="U8116_000287"
     CDS             2474..4105
                     /locus_tag="U8116_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEN8433308.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4167..4808)
                     /locus_tag="U8116_000288"
     CDS             complement(4167..4808)
                     /locus_tag="U8116_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN8433309.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4994..5070
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5090..5398
                     /gene="rplU"
                     /locus_tag="U8116_000289"
     CDS             5090..5398
                     /gene="rplU"
                     /locus_tag="U8116_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEN8433310.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5425..5769
                     /locus_tag="U8116_000290"
     CDS             5425..5769
                     /locus_tag="U8116_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEN8433311.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5782..6075
                     /gene="rpmA"
                     /locus_tag="U8116_000291"
     CDS             5782..6075
                     /gene="rpmA"
                     /locus_tag="U8116_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEN8433312.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6509..9316
                     /locus_tag="U8116_000292"
     CDS             6509..9316
                     /locus_tag="U8116_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN8433313.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9507..9998
                     /locus_tag="U8116_000293"
     CDS             9507..9998
                     /locus_tag="U8116_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN8433314.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10101..11090
                     /gene="manX"
                     /locus_tag="U8116_000294"
     CDS             10101..11090
                     /gene="manX"
                     /locus_tag="U8116_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEN8433315.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11123..11926
                     /locus_tag="U8116_000295"
     CDS             11123..11926
                     /locus_tag="U8116_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEN8433316.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11947..12861
                     /locus_tag="U8116_000296"
     CDS             11947..12861
                     /locus_tag="U8116_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEN8433317.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12944..13318
                     /locus_tag="U8116_000297"
     CDS             12944..13318
                     /locus_tag="U8116_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEN8433318.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13457..13702
                     /locus_tag="U8116_000298"
     CDS             13457..13702
                     /locus_tag="U8116_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN8433319.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13874..14938
                     /locus_tag="U8116_000299"
     CDS             13874..14938
                     /locus_tag="U8116_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN8433320.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15184..15609
                     /locus_tag="U8116_000300"
     CDS             15184..15609
                     /locus_tag="U8116_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN8433321.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15602..16051
                     /gene="nusB"
                     /locus_tag="U8116_000301"
     CDS             15602..16051
                     /gene="nusB"
                     /locus_tag="U8116_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEN8433322.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16148..17002
                     /locus_tag="U8116_000302"
     CDS             16148..17002
                     /locus_tag="U8116_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEN8433323.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17003..18340
                     /locus_tag="U8116_000303"
     CDS             17003..18340
                     /locus_tag="U8116_000303"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEN8433324.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18345..18569
                     /locus_tag="U8116_000304"
     CDS             18345..18569
                     /locus_tag="U8116_000304"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEN8433325.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18571..19461
                     /locus_tag="U8116_000305"
     CDS             18571..19461
                     /locus_tag="U8116_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN8433326.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19470..20288
                     /locus_tag="U8116_000306"
     CDS             19470..20288
                     /locus_tag="U8116_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEN8433327.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20395..20844
                     /locus_tag="U8116_000307"
     CDS             20395..20844
                     /locus_tag="U8116_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN8433328.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20862..22544
                     /gene="recN"
                     /locus_tag="U8116_000308"
     CDS             20862..22544
                     /gene="recN"
                     /locus_tag="U8116_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEN8433329.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22599..22715)
                     /locus_tag="U8116_000309"
     CDS             complement(22599..22715)
                     /locus_tag="U8116_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEN8433330.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22788..23744)
                     /locus_tag="U8116_000310"
     CDS             complement(22788..23744)
                     /locus_tag="U8116_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN8433331.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23915..24283
                     /locus_tag="U8116_000311"
     CDS             23915..24283
                     /locus_tag="U8116_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEN8433332.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24453..24884
                     /gene="mraZ"
                     /locus_tag="U8116_000312"
     CDS             24453..24884
                     /gene="mraZ"
                     /locus_tag="U8116_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEN8433333.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24905..25864
                     /gene="rsmH"
                     /locus_tag="U8116_000313"
     CDS             24905..25864
                     /gene="rsmH"
                     /locus_tag="U8116_000313"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEN8433334.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25869..26270
                     /gene="ftsL"
                     /locus_tag="U8116_000314"
     CDS             25869..26270
                     /gene="ftsL"
                     /locus_tag="U8116_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEN8433335.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26271..28463
                     /locus_tag="U8116_000315"
     CDS             26271..28463
                     /locus_tag="U8116_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN8433336.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28489..29451
                     /locus_tag="U8116_000316"
     CDS             28489..29451
                     /locus_tag="U8116_000316"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEN8433337.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29465..30826
                     /locus_tag="U8116_000317"
     CDS             29465..30826
                     /locus_tag="U8116_000317"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEN8433338.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30852..31940
                     /gene="murG"
                     /locus_tag="U8116_000318"
     CDS             30852..31940
                     /gene="murG"
                     /locus_tag="U8116_000318"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEN8433339.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31955..33175
                     /locus_tag="U8116_000319"
     CDS             31955..33175
                     /locus_tag="U8116_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEN8433340.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33331..34656
                     /gene="ftsA"
                     /locus_tag="U8116_000320"
     CDS             33331..34656
                     /gene="ftsA"
                     /locus_tag="U8116_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEN8433341.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34679..35920
                     /gene="ftsZ"
                     /locus_tag="U8116_000321"
     CDS             34679..35920
                     /gene="ftsZ"
                     /locus_tag="U8116_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEN8433342.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35937..36614
                     /locus_tag="U8116_000322"
     CDS             35937..36614
                     /locus_tag="U8116_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEN8433343.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36628..37227
                     /locus_tag="U8116_000323"
     CDS             36628..37227
                     /locus_tag="U8116_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEN8433344.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37242..37529
                     /locus_tag="U8116_000324"
     CDS             37242..37529
                     /locus_tag="U8116_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEN8433345.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37578..38360
                     /locus_tag="U8116_000325"
     CDS             37578..38360
                     /locus_tag="U8116_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEN8433346.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38381..39088
                     /locus_tag="U8116_000326"
     CDS             38381..39088
                     /locus_tag="U8116_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEN8433347.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39350..42148
                     /gene="ileS"
                     /locus_tag="U8116_000327"
     CDS             39350..42148
                     /gene="ileS"
                     /locus_tag="U8116_000327"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEN8433348.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42197..43723)
                     /locus_tag="U8116_000328"
     CDS             complement(42197..43723)
                     /locus_tag="U8116_000328"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEN8433349.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43738..44385)
                     /locus_tag="U8116_000329"
     CDS             complement(43738..44385)
                     /locus_tag="U8116_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEN8433350.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44569..44898)
                     /locus_tag="U8116_000330"
     CDS             complement(44569..44898)
                     /locus_tag="U8116_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEN8433351.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45075..45803
                     /gene="sfsA"
                     /locus_tag="U8116_000331"
     CDS             45075..45803
                     /gene="sfsA"
                     /locus_tag="U8116_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEN8433352.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45819..46832
                     /locus_tag="U8116_000332"
     CDS             45819..46832
                     /locus_tag="U8116_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN8433353.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46832..48109
                     /locus_tag="U8116_000333"
     CDS             46832..48109
                     /locus_tag="U8116_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN8433354.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48172..50874
                     /locus_tag="U8116_000334"
     CDS             48172..50874
                     /locus_tag="U8116_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN8433355.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51026..51343
                     /locus_tag="U8116_000335"
     CDS             51026..51343
                     /locus_tag="U8116_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN8433356.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51373..51693
                     /locus_tag="U8116_000336"
     CDS             51373..51693
                     /locus_tag="U8116_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN8433357.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51801..53261
                     /locus_tag="U8116_000337"
     CDS             51801..53261
                     /locus_tag="U8116_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN8433358.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53329..53550
                     /locus_tag="U8116_000338"
     CDS             53329..53550
                     /locus_tag="U8116_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433359.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53581..53763
                     /locus_tag="U8116_000339"
     CDS             53581..53763
                     /locus_tag="U8116_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEN8433360.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53763..54176
                     /locus_tag="U8116_000340"
     CDS             53763..54176
                     /locus_tag="U8116_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEN8433361.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54299..55480
                     /locus_tag="U8116_000341"
     CDS             54299..55480
                     /locus_tag="U8116_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8433362.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55551..55715)
                     /locus_tag="U8116_000342"
     CDS             complement(55551..55715)
                     /locus_tag="U8116_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433363.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55869..55942
                     /locus_tag="U8116_000343"
     tRNA            55869..55942
                     /locus_tag="U8116_000343"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55903..55905,aa:Arg,seq:cct)
     gene            complement(56011..57150)
                     /locus_tag="U8116_000344"
     CDS             complement(56011..57150)
                     /locus_tag="U8116_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN8433364.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(57449..58084)
                     /locus_tag="U8116_000345"
     CDS             complement(57449..58084)
                     /locus_tag="U8116_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433365.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58197..>58491)
                     /locus_tag="U8116_000346"
     CDS             complement(58197..>58491)
                     /locus_tag="U8116_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEN8433366.1"
                     /translation="FTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATP
                     KVSYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT        19224 a         9875 c        12443 g        16949 t
ORIGIN      
        1 gaggaaatag aattagaaac atataattat taaagggaaa catttttaca gctataattt
       61 gaaaaaattt atcgttatta tatgattaag aaggattgaa taaagaaaga agttgaaaac
      121 gatacctgat tacaaaaaaa taacttttat aagttctata acgttgatta tatttttaat
      181 tcttattctt ctcgtcgtct tggttccccg aatttttggt ttgactcctt atattattga
      241 ggacaaagaa atgagtccat attatgcaca aggaaatctg atatatgtac gaccgaaaaa
      301 tacaaatgag attttagtcg gagatattat tacatattat gaaaattcag gaaaaaaaat
      361 ctcgacgcgt cgcgtaatag ccgttgaaaa caaccacgaa gatttttata caaaagctga
      421 tacaaagact tatattgagc ctggagtagt aaaaaagaga aatattatcg gtagtcccat
      481 ctttcagatt ccatatattg gactagtcct ttcaaaaacg gcatttgcat ggattcaggg
      541 gttattcttt acgattgctt tttgcctaac aggaatcaca gcatggaata cctttgtaga
      601 gttgaaaaag aagcggagac gaacacagaa atttttgtaa gaatgttaaa aacataaatg
      661 tattccagag atccaacgac tcttctgaac atgaaacacg agaaaacact catgcagctt
      721 gtagatttag tcagttgttc gtataatgtg tgaagaggag gagtagtatg cgcaatgaaa
      781 tgatgcatcg tcctgtagtc aaagaagaac tattggattt tatgcgtacc cagcaaaaaa
      841 aattaccggg tgagctaggc aaactggaag aagaagctca tgtagcagaa gtaccagtta
      901 ttccccatga aacagttgta tttttgaaat ttttacttgg acagcttcag cctgaacgaa
      961 tcttggagat cggagcagct atcggttttt catcaagtgt catggccacg acaatcggag
     1021 aaaacgctca tgttacgaca attgatcgtt tcgatgtcat gatcgagaaa gctaaaaaaa
     1081 actatgaacg tctaggactg acagataaag tgactttgct tgaaggtcaa gcagcagata
     1141 tcttgccgga gttgtctggt ccgtatgact ttatttttat ggatagtgca aaatcaaaat
     1201 atattgaatt tttacctgaa tgcttacgtt tgttaagaaa aggtggcgtt ttgatggtag
     1261 atgacatctt ccaaggagga acgatcttgc tcccagatga agagattccc cgtggcaaac
     1321 gagctatcca tcgtaaatta aatgaatttc ttcgtgttgt gatggatcat cccgatttga
     1381 catcgacaat cttgccatta ggtgatggtg tgatcttaat gacaaaggaa tcagaaacaa
     1441 tcgagttgtg atacatgatt atggcagcct gtagataatc gattgaatcg agggctcaat
     1501 tcgaacttga tgtaaaagtt tctatatttg taagtcaatt aaaggataca ggaaatctaa
     1561 ataaacagga gtagcatgaa gtcaatgtag ctcctgtttt caattgataa ccttagaaca
     1621 gctgaaggag aatgtctcgt tttaaaagat tgatttagta gttgggccaa aataattcat
     1681 tatttgctaa ttaaataact atttcgtatt gacagaagca atttaattat ctataattat
     1741 tgatgttccg ctgattcttg tcatagtagc tcagctggat agagcatccg ccttctaagc
     1801 ggacggtcgg gggttcgaat ccctcctgtg acgctaaaaa accgttagaa actgaggttt
     1861 ctaacggttt ttctttgttt tttgtgccaa tattgtttgt tttcaaaggc aaataaaata
     1921 aaaaaacatc cttatcgagt acaattataa ggagcaaaaa aataaaactc atcagaaaag
     1981 aggaagaaga tatgacgata ctatctagaa ttttagtgac tctagttgca attgaatttt
     2041 tttatatcat gtatatcgaa acggtgagaa ctgattctga cacgacaagt agggttttta
     2101 aaatgagtaa agaagagtta agcagaaaat ctgttcaaac attgtttaag aaccaaggag
     2161 tgtacaatgg actattagga gtgggactac tttatggagc ttatctttct tccgcatcta
     2221 aagaaatcac aagtatgcta ttaatatcta ttttttttgt tgcattatat ggcagtttga
     2281 ctagcgatcg ttggatttta ctcaaacaag gtgggctagc tctgttggca ttgctttcta
     2341 ttttcttata aatatagaaa aaataagaat ttatctaaca ggaaactttg tgaaaaatat
     2401 tactttatct catgaaatat gatattctaa tgaatggtat tttagaaaat ttcatgaaaa
     2461 aggagttaga taggtgttca aaaattatgt ttcctcacta aaaaaagaat tttcaggtta
     2521 tgatctttca agatttcaga aagatctttt ggctgggatc acagtagctg ctgtagcatt
     2581 gcctttagcc ttggcttttg gggtatcaag cggagctgat gcggccgcag gtttgattac
     2641 ggctatcatt gccggtatcg ttattggcgg tttatcaggc ggtttttatc aaatatcagg
     2701 tccgacaggt gcgatggcag ctatcttgat gtctattgcg gcaacaaagg gaatgcaagg
     2761 tgtattgctt gctacttttt tagcgggtct atttttactt gcagcaggac ttttccgatt
     2821 aggaacactg acttctttta tcccagcacc cgtaattacg gggtttactt caggtattgc
     2881 tatcattatt gcattaggtc agatcgataa tttatttggt gttcactcgg aaggtacaaa
     2941 cgttattgaa aaactaagta gttatcaaac gataggattt ccgataaaca cagcctcttt
     3001 gatcatggga gggctagtca ttttagggat gtttatctat ccaaaaaaat gggcgaaacg
     3061 aatgcctagt tcgctgctgg ctatcatttt ggcaacagca gttatgctat tgttccattt
     3121 accagtagca actgtgggta agattccaca aacattgatc agtagtaatc gtttgaatat
     3181 gggagatttt agcttttctg ccttgcaaca agtggcagtt ccagctatta gcattgcttt
     3241 attaggaatg attgaaagct tgctatgtgg tgcttcggct ggccgaatgg caaataagcc
     3301 cttagatagc aatcaagaat taatggcgca aggaattgga aatctgttgc ttcctttctt
     3361 tggcgggatt cctgctactg cggccatcgc ccgtacaagc gtggccatca aatctggtgc
     3421 acaaactaga ttagctggta tgatacatgc agttgtctta ctgctctcca tgcttgtttt
     3481 tgcgccagtg atgtcacaga ttccaatgcc agcacttgca ggtgttttga ttgtgacggc
     3541 ttggcgtatg aatgaatggg aaacgatcaa ggagctattt actaataaat attggtcagc
     3601 agttctattg tttatcctta cgatgatttg tacagttatc tttgatttaa gtattgctat
     3661 cgtgatcggg gtgatcggcg gctgtgtatt ttttgttgtc aaaagcgctg ctatcacgat
     3721 ttcattagaa acgatcgatt ggcaaagaat ggggctgcca gaaacggaaa agctgaataa
     3781 ctggacagta gtgtatatta gtggtccact gttttttatg tcttcagaaa aactcaaaaa
     3841 taaattggaa gaactaaatc atcacgatgg tgtcattttc tccatgcgag gcgtaccaag
     3901 tattgatttg acggcacgtg cagtttttga tgaatttcaa gaaaaagcag agcaaagagg
     3961 acaaacgatt atctatactt ccttgcagcc agaagtagaa aaacaacttt ctcacttgtg
     4021 ggaacataca ttcacgcatc aacatcaaac agttgctcat gcgttgacag ctttacatgc
     4081 gaaatatact gcaattagcc attaaaacaa tgaaaaagaa cagacaagac tgtaaactga
     4141 agattttatg gtcttgtctg ttctttttaa tattgctgta aaagtgaagc tagtacttgc
     4201 cagtcttgat ggataacagg tacaagggag cgattgtaga aaatagctgc tggatgaaaa
     4261 agtggaagaa ttcgatagtt tttttctgac cattcgtagg catccttttg ttctgttaat
     4321 tgtaagacag gccctgtata gagttgacca tgatttttgg taaccgtata ttctttgcca
     4381 agtaaacgtt gaagaccaat gtttccaagt gtcgcaatta attttggctg gaccttcatc
     4441 agttcgtaat ccaatattgg tgcatgcgca aatacttcgg aacgagtagg tgtccgattt
     4501 ggataaacag tttctgactg ttgtgtccgt ttattgatcc gatgagtcac gcgatacggg
     4561 cggctcctta cagcactggt gatatacacc tcttctcgtg ttaagccagt tgaagaaagt
     4621 gcctgcatca attcttttcc agcttgaccg ctaaaaggaa catgcgtatc aatctctgtt
     4681 ttccctgggg cttctcccac gagcatcagt ttaggatgaa gaggtccttg cccagcgata
     4741 aatccttcta caggacggcc gttcattctt aaagaagctt cttgtatcag ttcttttggg
     4801 tattccattt atgctcctcc tttttctttt attataagaa taaaagaaac acaatgcgac
     4861 tattttattt ggtagaactg tgaggataga aaagcagaag tattgcttga atttttgttt
     4921 tactttttga ttttaaattt agttgactaa cggttgctgt tctggtaagc tagtactgtt
     4981 gtaattgtgg actccacagc tacaaccgct cagaacaagt aataagcaca gcatatgctg
     5041 tcacttcgga tggcgagtct aagtatatca gaaggaggtg ctttatagca tgtacgcaat
     5101 tattaaaact ggtggtaaac aagtaaaagt tgaagtggga caagcaatct atgttgaaaa
     5161 acttgacgtt gaagcaggag aaaaagttgt ttttgacgaa gttatcttag taggcggaga
     5221 atctacaaaa gtaggcgctc caactgttgc aggtgcaact gttgaaggta ctgttgaaaa
     5281 acacggcaaa caaaagaaag tcgttacttt caaatacaaa cctaaaaaac attcacaccg
     5341 taaacaaggt catcgtcaac catatacaaa agtggttatc gacgcgatca acgcttaata
     5401 gcgtttgaga aagaaggcct atatatgatc aaaggaacat ttaagcgaaa tgacgcaggt
     5461 cagatcgttt cattcacttt gaccgggcat gctgatgctg gtccttatgg aagtgatatc
     5521 gtttgtgcgg gtgtttctgc gttagcgatc agcactgtca acgggattgc ctcattagcc
     5581 ggttttgagc cgattgttga aatgaatgaa gaagaaggcg gttatcttta taccgaagtc
     5641 acttcaggaa tgacacaaga acaaaacaat attgctgaga ttctattaga aaatctttta
     5701 ttaggattac aatcgataga agctgaaaac tcagaatata tccaaattaa aacaattaat
     5761 gataaatagg aggtgcagac catgttgtta tctatgaatc tacaattatt tgcccacaaa
     5821 aaaggtggcg gttctacatc taacggccgt gactcagaat caaaacgtct aggtgctaaa
     5881 cgtgctgatg gacaaacagt tacaggtgga tcaattcttt accgtcaacg cggtacaaaa
     5941 atctatccag gtgtaaacgt gggtatcggt ggagacgaca ctttatttgc aaaagttgat
     6001 ggcgtagtac gtttcgaacg taaaggccgt aacaaaaaac aagtgtctgt ttatccagta
     6061 gctcaagaag cttaattgaa tcttatgatt aaagaggttg tgacaagact tttgtcacag
     6121 ccttttttgt ttataaaaat aaaagttgta gtggaggcta cgaagtttac tttgtctttt
     6181 tacacttaat atttttttgc caatttggaa tcaggatgtt ttgataacta tcgttttcaa
     6241 ttgatggaag ctatattgtt tgctatcagt gaaataatta tatggtaaac agtgttcttt
     6301 ttcttgtttc cattataaaa atcgtatcgg gatttttcgt tcttttaaat tgaacatttc
     6361 tatctatccg cgtgttatca acaaaacaaa atcaggtaaa tagttgacga aacagaaata
     6421 aatacatagt attatgtttt gaagcctaag tacaatagtc tatagaaaag gagcgatacc
     6481 attggcaaaa cgcattgacc gaatttatat gtatgtcaaa gaaaaaaccg ctcatttaac
     6541 aaaggcagaa tatgatcaag gagtaacaac acaggaaatt gctgaagtgt tagggattca
     6601 gcgaacaaat agcagtaaag atctgaatca gctagtgcgc gaaggaaaac tgataaagac
     6661 agacggacga ccagtacgtt acatatatca aacactcgtt acgcacgtaa agccagttac
     6721 aaaacatgta gtcagttaca aagaaacgcc ggtctctgaa gaaaaaaata cactccaaat
     6781 agatactaag gatatctttg caaaaatcat tggagctaac ggcagcatga aaaattcagt
     6841 ggaacaggca aaagctgcga ttttgtatcc gccaagagga ttaaattgtt tgattacagg
     6901 acctaccgga tcaggaaaga cttattttgc acacgcgatg tatcattttg caaaagcgca
     6961 tcatgtaata gatgaggaaa cggaactgat tgttttcaat tgtgctgatt atgccaataa
     7021 ccctgagctt ttgatgagtc atttatttgg gtacgcaaaa ggtgctttta caggcgcaga
     7081 tgaagaaaaa atggggatta tcgatcaagc ggatggcggt atgctgtttt tggatgagat
     7141 tcaccggttg ccgcctgaag gtcaagaaat gatcttttac tttatggatc atggaactta
     7201 cagtcgtctt ggggaaacaa cgaaatccca tgaagcaaat gtacggatcg taggtgcgac
     7261 cacagaagac ccaggttctt ctctattaga aacttttgtt cggcgtatcc caatcaatat
     7321 caaacttccg gcttttgaaa aacgtcctgc aaatgagaaa gtcgatttag taaaaatcat
     7381 gattgcccac gaagcaaacc gcacgcagcg aaagatttct ttgacagaag atgtagcaaa
     7441 agcattgatc ggcagtgtga cctatggaaa tataggacaa ttgaagtcaa atatccagtt
     7501 agtatgtgca cgaggatttc taaatcatat gcaatcaccc gaaatctcga tcacaatcga
     7561 tgatttgaca gaagggattc gtagcggatt gattcaatta gcaagcaacc gcagtgcaat
     7621 ggcagagctt tctaaaatat tggagccaaa gataactgta gcaccaaatg atacgatcat
     7681 gaaaatccaa tcggactcct atgagttacc ttataatcta tatgatatca tcggagataa
     7741 agcagcttta ctgaagtcgg atggtttgga tcaagaagca atcaatcatt tcatttctac
     7801 agatatcaac atccatctga agtctttcta taaagaccat ggtttttctt ttaatgcaga
     7861 cagcaaatta gcagaatttg tagatccgaa aatcatagaa gtgaccaatc agatttatgt
     7921 aatggtcaaa agatctttac catatgagtt tcagcaaaac tttatttatg ctatgagctt
     7981 gcatatcagt tcttttttga aaaaaatcaa cttaggagaa gaacggcata cgaatgataa
     8041 catccgtgag atggcttttg attttccaga tgaatatgct gttgcgaaag aagtgcgtag
     8101 ttatatcgaa acgtattttc aagtggagat tccagaaagt gaagactatt acttgaccgt
     8161 tctactcgtc tcgttacggg caaatcaagc aagcggcaga atcggtgtag tcgttgcagc
     8221 tcacggaaac agcacagcaa gcagtatggt ccaagttgtc caacagttgt tagatgcaga
     8281 gaacgtacga gcagtagaca tgcctttgga tatggatccg aaaacagcat tggaccgaat
     8341 tgaacgcaat gtacaagaag tagatgaagg aagcggggtc atactcttag tagatatggg
     8401 gtcccttgcg tcgtttaatt cgcaaattca aagagacact ggaattccag tacgtactgt
     8461 tgatatggta acaacttctc tagttttgga aactgtacga aaggtctcag tattagggac
     8521 agatctagat atgttgcatg aatcattgaa aaattttcga ggttacgcag aaatatcttc
     8581 tgtagaatct gttcaggaag caaaacctat tcataagaaa aaagctgttt tggctatctg
     8641 cgcatctggc gaaggtacag cacaaagaat caaagaattg atcgaacgag cggtaaacaa
     8701 gcgtcaagaa acagaattag aagttctagc tttgtcgatt gtggaagtga aagaagaact
     8761 gccagttatc caaaaagaat atcagatcat agctactact gggattactg atttgaatat
     8821 ccaagcccca tttattcctt tagaacgttt tattgatcaa aatatcgaag tcattttgga
     8881 tcagttgctg atggaatcag aattggagga aactgaattc atctccctag atgaagagtc
     8941 agctaaaaat acgtgtgttg agtttatctc tgataacttc atctttatta atggtagtaa
     9001 gttaatcgat ccaatgtggc aattttctac acaaatctct cagacgacgg gaatcggcga
     9061 tgaagaatat ggattcaaaa tcaacctagt gatgcacaca gcaggaatga tcgaacgtat
     9121 tattcggaat gaaccattga ctgtggaaga aaacgaattg acaaatacaa cgaatgatcc
     9181 tctttatagc caattggcag cctcagttgt tctgttggaa gatcagataa aagtaaaagt
     9241 gccgatagaa gaaatgtatt atctgctacg tctagtccat aatcagttag ataaaaaaga
     9301 atacactgtg ccatgaatac acaaaaacga tacactttta tagtgtatcg tttttgtgta
     9361 ttggttaaga atcctttaaa ttcaattagt gtatcgtaaa aaagttggca cgctttttgc
     9421 ttatagtagt gtgtgctagg aggtggttgt tcacacagaa atgatagggg tttctgtgaa
     9481 gtagtgagga agaaggagtt ttgcagatgg atattcgatt ggcacgtata gatgatcgtt
     9541 taatccacgg gcaagtcgca acagtttggt cgaagcaaac aggtgttgaa cgtatcattg
     9601 tgatcagcga tgtagtcgct caagataaat tgagaaagtt tctactaaaa gaagctgcac
     9661 cgccggggat cacagcaaat gtaataaccg tgcagaaaat gattcaaatc tatcatagtg
     9721 gattattatc acgagcaaaa gtgatgcttt tattcaccaa tccccaagat gttgaagcgg
     9781 ttgtccgctg cggtgtacag ttgaagtcgt tgaatatcgg cggtatgagt tttactgaag
     9841 gcaaatcaat gattaccaat tttgtttcag taaatcaaca agatattgat tcctttcgtt
     9901 acttgaacag tcaagaagtg gagttagaga ttcgaaaagt acctgctgac aggaaaatcc
     9961 ttttgatgga actactagcg aaaatcaaaa aggtgtagca agaatggttg ttggaaaagg
    10021 tcaaataatt ctgttgtaat ggttagcaat tattaatatt tttgaatttg ttacaacaaa
    10081 agtaaatagg aggtataaat atggtaggga ttatcctagc aagtcatgga caatttgctg
    10141 aaggaatctt acaatccggt tcaatgatct ttggcgaaca agaaaacgtc aaagccgtga
    10201 ttttgaaacc aagtgaaggt ccagatgact tgagagccaa attggaagaa gcggttgctt
    10261 cttttgataa tcaagacgaa gtgttattct tagttgattt atggggcgga acaccgttca
    10321 atcaatcaaa cacattattc gaagagcaca aagataaatg ggcaatcgtc agcggtttga
    10381 atttaccaat gttgattgaa gcctacgcat cacgcttttc aatggaatct gctcatgaaa
    10441 ttgcagcaca catcattgaa acagcaaaag acggtgtaaa agtaaaaccg gaagaattag
    10501 aaccggcaga agcaccgaaa gcagcagttg aagacgcaca acctaaagga gcacttccag
    10561 aaggaactgt ggtcggagac ggaaaaatca aatatgtatt agcacgtgtt gactcacgtc
    10621 tgttacatgg acaagttgcg acagcttgga caaaagcagt gcaaccaaac cgtattatcg
    10681 ttgtttcaga tgcagtatca aaggatgatc tgcgtaagag actaatcgaa caagctgctc
    10741 ctccaggtgt aaaagcaaat gttattccta ttagcaaaat gatcgaagta gcaaaagacc
    10801 ctcgttttgg aaataccaaa gcattgcttt tatttgaaaa tcctgaagac gtattgacag
    10861 ctgttgaagg tggcgtagac atcaaagaat taaatgttgg gtcaatggcg cattcggtcg
    10921 gtaaagtcgt tgtaagtaaa gtattgtcta tgggacaaga agacgttgaa gcgtttgaga
    10981 agcttgaaca aaaaggtgtg aaattcgatg tacgtaaagt acctaatgat tcacgcgata
    11041 acatggatga tattttgaaa aaagcaaaag ctgaattagc aaaagcgtaa taaataatat
    11101 taaaatttat aggaggctta tcatgtctat tatttcaata attttagtcg tattagttgc
    11161 ctttctagct ggtatggaag gtattcttga tgaatttcaa tttcatcaac cactagtggc
    11221 atgtacattg atcggtttag ttaccggtaa cctcgaagct ggtatcgtac ttggcggtac
    11281 gcttcagatg atcgctttag gttgggcaaa tatcggtgcc gctgtagcac cagatgctgc
    11341 attagcatca gtagcatcag caattatttt agttttaggt ggacaaggtg ttaaaggtgt
    11401 tccttctgcg atcgcgattg ccgttccttt ggctgttgca gggttgttct taacaatgat
    11461 cgtacgtaca atcgctgttc caatcgttca tttgatggat gcggctgctg aagaaggaaa
    11521 catccgtaag gttgaaatgt ggcacatcat tgctgtatgt ttacaagggg ttcgtatcgc
    11581 ggtcccagca gcagcgttgc tatttatccc agcacaaact gtgcaatcat tccttgaatc
    11641 aatgccagca tggttaacag atggtatggc tatcggtggg ggaatggtcg tagctgtagg
    11701 ttacgctttg gtaatcaaca tgatggctac aaaagaagta tggccattct tcgttattgg
    11761 tttcgttgta gctgcaattt ctcaattaac attgattgct ctaggtgctt taggtgttgc
    11821 attagctctt atttacttga acctttctaa aatgggtggt tcatcaaata atggaggcgg
    11881 aagcaactct ggtgaccctt taggcgacat tttgaatgac tattaatctt gaaggaggaa
    11941 aataaaatgg cagaagaaaa aatcaaatta tcaaaaaaag atcgtttagc tgttgcgtgg
    12001 cgctctacct tcattcaagg ttcatggaac tacgaacgta tgcaaaacgg tggctgggca
    12061 ttttctatga ttccagcaat caaaaaatta tacaaaacaa aagaagaacg ttctgcagca
    12121 ttaaaacgtc acttggaatt ctttaataca cacccataca ttgcttctcc aattttaggg
    12181 gttacattgg ctctggaaga agaacgtgcg aatggcgcgc ctgttgatga tgtagcgatt
    12241 caaggggtta aagtaggtat gatgggtcct ttagctggtg ttggtgaccc agtgttctgg
    12301 tttacagtac gtccaatgct aggtgcacta ggtgcttcat tagctatggg aggaaatatc
    12361 cttggaccaa tcatcttctt cttaggttgg aacattattc gttgggcatt catgtggtat
    12421 acacaagaat ttggctacaa agctggttct aaaattactg atgacctttc tggcggattg
    12481 ttacaagacg taacaaaagg tgcttcaatt ttagggatgt tcgtattagc tgcccttgta
    12541 caaagatggg tatcaatcaa attcttacca gttgtatcaa ctgtaaaatt agataaaggt
    12601 gcttacatcg aatgggataa attgccttca ggtggtgaag gaatcaagag tgcatttgaa
    12661 caagttaata atggcttagc cctttctcct gaaaaggtaa caacattaca agacaactta
    12721 gaccaattga ttcctggttt agctgcatta ggattaacat tgttatgtat gtggttgctt
    12781 aagaaaaagg tttctccaat cgttatcatc cttggtttat tcgtagtcgg tgttgttgga
    12841 cacgtcatcg gacttttata atccataaaa atatagctcg ggcgttgtcc cgagcttttt
    12901 ttatatagta tactgttcat aaacaaatat ctggagggag aaaatggttc aatcaatcaa
    12961 tacaaaagta gacttggtaa ttgatgctac ttcgtttacg ggtctgacag attacgggca
    13021 aatcatgata ggtaataatg gttttgagtt ttaccatgcg cgagatagac gcaaatatat
    13081 ccaaattcca tgggaagaag tggattatgt actagcatca gtcatgctaa agggaaaatg
    13141 gattcctcga tatgccattc aaacgaaaaa aaatggtaca tacacatttt cttccaaaga
    13201 acctaaaaaa gtgcttcgtg cgatcagaga atatgtagat gaagatagaa tggtacaatc
    13261 attgagtttc tttgatgtag tgaaacgtgg tattcgtagt atgtttaaac gcaagtaaaa
    13321 aataaagaaa acttctaaga ggaggttttc tttatttttt tactttgcag aagaaaatat
    13381 gaaatagcaa aaccaattat ttgtcccctt taaatcccat atgctaaaat tgattagcaa
    13441 agaaggaggt tatattatga gttggttatg gacattgatt gttggagccg tgatcggagc
    13501 aattgcaggt gcgatcacca gtaaaggaaa ttcgatgggc tgtgtaacga atatcattgc
    13561 tggactagtc ggttcaagta ttggacaagc gattttcggc gaatggggac caaaagctgc
    13621 aggtatggca cttattccat ctattttagg cgcagtgatt ttggtagcag ttgtctcatt
    13681 tttctttgga aaacgatcat aagtttttac aaaatagtaa actaaaagga gttgcagcga
    13741 gactgtgact cctttttggc tttatttatt taaaataaaa agaagttttc cggaaatttt
    13801 tatttaactg tcgtgtacga agaaaagttt gctataatgg aaaaaagcgt gcaaagaaag
    13861 agggagtttc attatgatgc tacgtgtaga aaaattgcgc aaaaaaatgc aagaagaaaa
    13921 tttggattca tttttggtta ccagtccgta caatcttcgt tacttaacga actttacagg
    13981 tacgacagga ttagctgtta ttactttgga aaaagcattt tttattactg attttcgtta
    14041 taccgagcag gcggcagcgc aggctcaagg gtttgaaatt attaaaaatg tcggacctat
    14101 ttttgatgag gtagcaaact tagtacgcaa agaagaactg cactcattag cttttgagga
    14161 aacaacagtc tcatttttag aatattcggt tttagaagaa atcatcgatg cggaattagt
    14221 acctgtttca cagatgatcg aagaactcag agaagtgaaa gatgaagaag aaatcgctat
    14281 tatcgaaaaa gcatgtcata ttgcggatat ggcatatgat cacatcctga aaatgatcca
    14341 accgggcatg actgagattg aagtcgcaaa tcaactagac ttttatatgc gttctcttgg
    14401 cgcttcaggt gtttcttttg atacgatcgt tgctagcgga ttgcgttcgg ctatgccgca
    14461 tggtgtagca agcaaaaaaa tcatcgaaca aggtgacttg atcacattgg actttggttg
    14521 ctattacgaa gggtacgttt ccgatatgac acgtacattc gctattggtg atcctgggga
    14581 aaaattaaaa gaaatttatc aaatagtctt agatgctcaa ttagcagtta ttgatgctgc
    14641 aaaaccagga atgactggtg tccaattaga tgcagttgct agagattata tttcaaaaca
    14701 tggttacggg gaagcatttg gacattctac aggacatggg atcggtttag agatccacga
    14761 aggaccaaac gtttcttctc gtgcagaaaa gcaatttgta ccaggaaatg tgatcactga
    14821 tgaaccaggg atctacttac caggaatcgg tggagtacgt attgaggatg acttgttgat
    14881 cacagaagat ggaaatcgtg tgttgactcg ttcaccaaaa gaattgatca tcttatagtt
    14941 tcatcaatag aagaagtaag cgtaaaaatc agtgttcaca gactttgtta tagaaaatca
    15001 ctcgatttaa tgatacacta tagaagaact tttaagctgt aagagataat aaagttgggt
    15061 gttttctcca attcatagca gtttgcagct gatcggcggc agttttctta ccacgtgatc
    15121 actatgccgc atcatagaga attcggatgt atctcgtgct taggcaaagg gaggaataaa
    15181 taaatggcag atgaaaaaaa tttgatttta aacgcaaacc aagatttagg agagatcgtg
    15241 atcgctcctg aagtcatcga agtaattatc gggattgcag cttctaaagt agaaggcgta
    15301 tacggaatgc gcggaacttt tgccagcaat gtcactgaat tattaggacg tgcagctcac
    15361 ggaaaaggtg tttacttagc aaacgaggaa gatggcttga aagtagatct ttactgttat
    15421 ctagaatatg gtgtttcagt tcctaaagta gcaatggaaa tgcaggaacg ggtcagccag
    15481 caagtccttt tcatgacgga tatcgattta tctgaagtca atatccatgt agtagctgtt
    15541 gttcctgaaa aattagaaca accaacaatc gaagaacttt ttgaagacga agaggaagag
    15601 aatgaataaa gaattatcaa gacacgagat tcgggaaatg gcattgcagg ctttatttcc
    15661 attagacttc aatgcagacc taacaaaaga agatgcgatt ttcaacgcaa tcgaattaga
    15721 ccaccgcgac atgatcaacg aagatgaatc agagtttgtt cctgtttatc ttgatacgct
    15781 agttggcggt gtctgtgcaa aaaaagatga attggataaa gtgattgaaa aacatttaaa
    15841 aaacaattgg cacatcaatc ggatctcaaa aatggatttg gtgatcttgc gtatcgcgat
    15901 ttttgaaatg acttatgtga cagatgtacc agcggctgtt gctttaaatg aagcaatcga
    15961 gttggcaaaa acatttagtg atgaccgttc acgtaaattc gtcaacggtg ttttatccaa
    16021 tgtattgaaa gaattagaag ctggggcgta agtcctagct tttttctatg aatcatcata
    16081 aacctgttat aaatcgggaa aattctatgg tagaatgaaa acacaaaaaa tatctgggag
    16141 tggttagatg gcagaattga ttaacggaaa agaattagct gaaaaaatgc aggcagagat
    16201 tgctgaaaaa atccaaaaat tgaaagataa tggtatccat cccggattag tcgttttatt
    16261 ggtaggggaa aatccagcca gccaaatcta tgtaagaaat aaagaacgtg tagcaaaaga
    16321 aatcggaatc cattcattgg tagagcgtta tcctgagacg atttctgaag acgaactact
    16381 tgcagaaatt gaaaaataca atcaagatcc tagttttcat ggtatattgg tccaattacc
    16441 attgccaaag catatagatg aagaaaagat cctgttagct atccgccctg aaaaagacgt
    16501 agacggtttt catccgatga atttaggcag actccttgcg ggtaaaccgg acaagattcc
    16561 ttgtactcca tacggcatca tgaaaatgtt tgaagcttat tctatcgatc cggcgggtaa
    16621 acgagcagta gttatcggac gaagcaatat cgtcggtaaa ccgatggctc agctactctt
    16681 gatggcagat gcaactgtta ctgttgctca ttctaaaacc gaaaatctgg ctgaactggc
    16741 acaagaagca gatattttag tcgtagcaat aggacgtgga cattttgtca caaaagaatt
    16801 cgtaaaacca ggtgctgtag tgatagatgt agggatgaat cgtgatgcta acggtaagct
    16861 gatcggtgat gtgaaatttg atgaagtgga gcctattgca ggctggatca ctcctgtgcc
    16921 aaagggtgta gggccaatga ctatcacgat gctgatgtat caaaccgtaa aatctgccga
    16981 agcttttgga gcaggtgaat agatggcaca agaatattta actgtcacta cattaacgaa
    17041 gtatttgaaa agaaaatttg atgccgatcc ttatttagaa cgcgtatatt taacaggcga
    17101 aatatcgaat ttccgtttac gtgccaatgc ccatcaatat ttcagcttaa aagatgacca
    17161 tgcaaaaata tctgctatta tgttcaaagg agcattccaa aaattacgat tccagccaaa
    17221 agaaggaatg aaagtccttg tcgttgggcg tatttcatta tatgaagcgg gaggttctta
    17281 tcaaatctat gtagagcaca tggagcctga tggaataggt gctttatatc aagaattgga
    17341 agaacgaaaa gaaaagctga tgaaggaagg cctttttcaa gggcctaaaa aaatgctgcc
    17401 acgtttccca aagcgaatcg cggtccttac cagtccaagc ggagcggtta tccgcgatat
    17461 catcacgacg gtgaaaagaa gatatccaat tgtccaattg gttttgttcc caacagttgt
    17521 acaaggaaac caagcagcgg acaatgttgt caggaatatc cagagagtag aagagatggg
    17581 caatttcgat acaatgatca tcggtcgtgg gggtggttcg attgaggatt tatggccgtt
    17641 taatgaagag cgggttgctc gggcgattta tcaagctaat acgccggtga tttcatctgt
    17701 aggacatgaa acagatacca cgatcgctga tttagtagca gatgttcggg ctgccactcc
    17761 aacagcagct gcagaattag ctgttcctgt tttgtcagaa gagttgatgc gcattcaaga
    17821 acggcaagct agattgcagc aggcgtttgt acgccagatc cagcgcaaac aagaacggtt
    17881 cgagcgtgct cgacgttcct atgtatttcg gcagcctgaa agattgtatg aaggtcagac
    17941 catcaaatta gaccagttga aacagcgatt atatcaagct acacagcaaa tttatcatgc
    18001 gaaagaaaaa caaacgatcg cattaagcca ccaattggaa caagtggctc cgctttatcg
    18061 agttaaagca gctcgccaag aaactgctta tctagaaaaa cgcttagaag agaaaatcca
    18121 acaatatatg aaacaacagc agcagcgttt tgctcaagct gttcaatctc ttgatttgtt
    18181 gagtccattg aaaatcatgg gaagaggcta cagctatacg acgttaaatg atcaagtagt
    18241 aaaaagtgtc accgagattc atcaagggga tttgctgacg atccattatc aagatggaat
    18301 cgttaaagga caagtacaag agatagacaa aggagaataa aaatatggaa aatccaactt
    18361 ttgaagagtc actgcaagaa ttagaaaaaa ttgtcatgca gttagaacaa ggagacgttc
    18421 cattagaatc agctctagat tcgtttaaac gaggaatgga attaagcaaa cattgccaag
    18481 atacattagc taaagcagaa gagaccttga cgaaaatgat gacagaatca aatgaagaag
    18541 ttgattttga cggaaacgag gaaagctgag atgagattaa ctgacttttc agctattcat
    18601 ctacctgctg tagaaaaaga aatcttgtct tttttagatg agcatacaac ggatagagga
    18661 ctctatgatg cgatgagtta ttcagtgaaa gcaggcggga aaagaatacg tcccttatta
    18721 cttttaacgg ccgttgcttc ctttgatgag cctattgatg ttcccgtcta tcaagtagca
    18781 gctgctttag aaatggtgca tacgtattcc ttgatccacg atgatttgcc tgcgatggac
    18841 aatgatgatc ttcgtagagg aaaacctacc aatcataaag tattcggcga agcattagct
    18901 atattggccg gagatggttt gcttacagga gcattccagc taatcagtat ggcacactta
    18961 ggtaattctc ctaagcttct gctgcttcag caattagctg tttgtgcggg atcacaaggt
    19021 atggttgctg ggcaagctgc agatatcgag ggcgaatcga aaaagctttc tttagaagaa
    19081 ttagcattta ttcatgaaag aaagacaggg catttgattc gttatgcctt gctggctggt
    19141 ggcatattag cgaagcaacc tgaagaaatc ttattgctac ttcaacgatt agccgaacat
    19201 ttaggtttag cttttcagat aagagatgat ctactcgatg tgattggtac aacgaaaaca
    19261 ttagggaaaa cagctggtaa agatgaacgt atggaaaaaa atacttaccc gcgtttatta
    19321 ggcctagaaa agacacgtga ggcgttggag attgagctgt tatccgccaa caagatcata
    19381 gacaaattag aagaggacgt tttttctttt gatggcgggc tgatgcgcca gatgatcaag
    19441 caatttgatg tggagaaatg agggaaagaa tggaaaaaga acgcgtagat gtattagcgg
    19501 tcaaacaagg attatttgaa acaagagaac aagccaaacg ttcagtgatg gctggtttga
    19561 tttacaacga aaaaaacgag cggtttgata aaccgggaga aaaaattcca gtgagtagtg
    19621 aattaagagt caaaggtaaa aaacttcctt atgtttctcg agggggatta aaattagaaa
    19681 aagcactcaa agcatttgat ctatctgttg aaggaaaaac attgttagat attggtgcat
    19741 ccactggcgg atttactgat gcagctttgc aaaatggcgc aaaaatgagt tatgctttag
    19801 acgttggcta caatcagcta gcttggaaaa taagacaaga tcctagagtc gtcgtaatgg
    19861 aaagagtgaa ttttcgttat gcaaaacccg aagactttac cttaggaatt ccagaagttg
    19921 ctgttatcga tgtttctttt atttcactaa aactgatgct gcctccatta catgctattt
    19981 taaaagaaga gggagaagtg attgctttaa tcaaacccca attcgaagca ggcagagaag
    20041 ctgtagggaa aaacggaatt gttcgtgacc ctcagaccca tcaaaaagtt ttagaagaca
    20101 ttttgtcatt tgcggcacaa ggtggatatg atgtgctgga attaagttat tctccgatca
    20161 cggggggaga aggaaatatc gagttcttgg ctcacttgag gaaagtaccc gaaagcggaa
    20221 caatcaattc agctattaat atggcagaag tggtttcaaa tgcacacgaa cagtttgacc
    20281 ataaataaat tgtaactgtg aaaatcaagt gagaagtatg cggattcttc tttattatac
    20341 caaggaaata agctatcatt gattaagaag aatatgctaa ggagcgaaga cgtaatgaga
    20401 aaaaaagatc ggcatcgttt gattactcga ttgttgagtg agcaagatat ccgaaagcag
    20461 gaagaatttg ttgaaatcct gaaaagcaaa ggaatatctg ttactcaagc tacgatttcc
    20521 agagatataa aggaactgaa actgatcaag gtaccggctt cagatggcgg ctaccgttat
    20581 agcataccag ctgaaacaaa cgaagatgta aatactaaat tggaaaaact attaaaagac
    20641 gctttcgtgg cagttgacca aatggaaaaa tttgttattt tgaaaacatt gccgggaaat
    20701 gcttcggcag cagcaaattt gattgataaa cgttacaaga aagaattatt ttcgattatc
    20761 aatgatgacg ataatgtttt gatgattaca cgaactgaag accaggcagt tttattgaag
    20821 aaagacttcc tgcattacct ttaagttgag gagcgaatgt gatgctacaa gaaatcagta
    20881 ttactaactt tgcgattatt ccggaattgc ggctttcttt tcacgaagga atgaccgcgc
    20941 tgaccggaga gactggtgcg ggtaaatcca ttattatcga tgctttaggt ctgttagcag
    21001 gaggaagagg ttcaagcgat tatatacgtc aaggagcaga aaaatgtgtg cttgaagggt
    21061 tgtttgaact accaaaacaa gaaggattct ctgaactgat ggttgaatta gggatcgaaa
    21121 cagatgagga caatctgatt gttcgacggg atatgtcctt aactggaaaa aatgtttgtc
    21181 gagtgaatgg tcatattatc acattagcta atctgagaag aatcggcagt tatttagtag
    21241 atatccaagg acaaaacgaa catcaggaac tgctccagcc tgaatcacat cttgctttat
    21301 tggatcgttt tggagatgta gctttccaac agaagaaaaa gagttatcag caagaatata
    21361 tctcttatcg tgagctggaa aaaagggtac gaaaagtgca gcaaaatgaa aaatcctatg
    21421 ttcagagaat agatatgctc catttccagc aagaagaaat agctgctgct gatttgcaag
    21481 tcggagaaga agagaaatta aaagaagaaa gagaaaaact tagcaattat caaaaaattg
    21541 ctgatggatt agctgcaggt tacggagcac tgacgaataa tgaacaaaac agtgtagatg
    21601 gtgttggctt ggccgtttca gaactgcaag ggatcgccca tctagatgtg gagtatgaag
    21661 caatttatga aaatatccaa agtgcttact atttgctgca agatgcaatc ggcgatatga
    21721 gtcgtcagat tgatttattg gagttagatg agagtcgttt agaagaagtt acgcaacgtt
    21781 tagaattaat ccgacagttg aaacgtaaat acggcgaatc cattgagtca atattggctt
    21841 actatgacga aatcaccgaa gagctagctt cttctgattt ttcagaaggt caattggaca
    21901 aaatgaaaga ggaactagag caaaaagagc ttctacttca gcaacaagcc gcagatcttc
    21961 atgaagcgcg taaaaaaatc gcaaaagaat tggaaaaatc cattctgcat gaattgaaaa
    22021 gtctgtatat ggaaaacact gagtttgagg tacgtttctt aaaagaagaa aatagacaat
    22081 tgaatcatga tggatttgac caaatcgaat tttacattac gaccaatcca ggagagccac
    22141 tgaagccttt ggtgaaagtc gcttctggtg gcgaactgtc tcggatgttg cttgctttga
    22201 aaactatttt ttcttctgag caaggtgtga caagtattat ttttgatgaa gtggatacag
    22261 gtgtgagcgg aagagttgca caggccatcg cagataaaat ttctcaaata tcgaaatact
    22321 ctcaagtttt gtgcatcact catctgcctc aagttgctgc agtcgccgac tatcaatatt
    22381 atattgtgaa agcagtgatt gatggacgta cgcaaacttc tgtttcagag ttgaaaacaa
    22441 aagaaagaga agaagaaatt gcacgtatgc tggctggaag tgagattaca aaactcacac
    22501 tcgaacatgc aaaagaacta ttgaaactcg caaaaaggac ctgagccacc tcgcagtggt
    22561 tcaggtcctt ttcagcagct gatagctata ttttattttt acatgacact cattacagca
    22621 gtcaatacaa cagatccaat cgttaaaatc agcattgtcc aaatgacaaa ttttgttact
    22681 ttagcaaaag tacttgtttg ttttttttca cgcattgttt tcaacctcgt cattaattag
    22741 ttgattcaag tgtacaatct tttttccatt tccacaagag ggctttttta taaaaacttc
    22801 ccttttttta aataataggt agttacgata caaataaata atgttaatag aactagccat
    22861 ataaaggcat catccctttc agcaataggt aacttaatgt tcattccata gatactgccg
    22921 acgattgtcg gtatagttaa aataatcgtt aaagaagtta aaattttcat aatattattc
    22981 agattattcg atacgattgc tgaaaaagtc tgattcattt gttctactag ttttaattga
    23041 atgcgagaag tagtcattgc ctgctctgtt tccaccaata catcacgtaa atgatttttc
    23101 aaagcatgat tttcttggaa aagtttattt tgcgctagtt ctttcaatgc atctaaatta
    23161 gcagaagtcg cttctctgaa ataaacgagg cttttttgga tatccatgat ttgataaagt
    23221 tgtttgtttt cagtggatac ttgaagtttg gcttccaaag tatcgcattg agattgaagc
    23281 gtagctaaat aactgttgta agagagtgtg acctgccaaa tgaattgaaa gaagatattg
    23341 agattaggcg aaatatcact ttttggaaag gtctggctaa agactttctt taaaaacagt
    23401 ggttcatgat tcgtaattgt gataagcttc tttttgggcg tcaaaataat agagagcggg
    23461 tatgtatcca cttgaaaata accgctagga cttgttttag caaaaggata ttgtaacaat
    23521 aataacgctg gctttgtcaa ctttgattga ttgaattctt ctgtacggga attttctgca
    23581 tcgtccaata tggaagtgat gtaatctgac ggaatttcaa attgatgtgt cagctgatga
    23641 atctcttctt gattaggacg ttctacacaa taccagatcg tttggtcagt ttcctttccg
    23701 gtattacgca gtttctcatt atctaatgta aaatatttga tcatgatttt cctcctgttg
    23761 cctgtttctt ttattataac ggatagggaa ttggaataca tggaatgtgt ttgtaagata
    23821 tagaatataa ctgcataaat ttaaataaaa tgaaacagtt gtgtaaagta tatacagtat
    23881 acttgaagca acgataaaaa aggagtttac gttcatgggc acattcacac cacttatctt
    23941 gttttggtat atttacccgg taatcgtttt gtttggttgc caatttcttg taaaattatt
    24001 ttcgttaaat cgtcgtttta aaataaaagc ccctgatttg gcgattcctt ttttgtgggg
    24061 cggtatgcat gcattgtcta gaaatacagg gacaatttct atcttaccgt ttttctttat
    24121 ttctgttttg ctaatgggaa tttgtattgc tgttttccaa gcatattatt atgaagaaat
    24181 tatttatccg cgttatttca agatgacttg gcgtttggtt tttctgttga caatcgtctt
    24241 gtacgctgtc ttgatcattg tgaacatact ttcttaccta tgaaaaaaat agaaaagctg
    24301 taaaaagtct tttaaaaggc tttttgcagc ttgattttta aaaaaatgtt tatgaataaa
    24361 aaaagttgtt cccggatttc ccagaaaagg tggtagaaag tgggggattg tggtagacta
    24421 attttagtta gtggaggaat ggggggcttt caatgttgat gggtgaattc caacataata
    24481 ttgatgcaaa aggacgttta atcgtgccct caaaacttcg tgaagaactt ggcgaaaagt
    24541 ttgttctgac acgcggattg gatggctgtc ttttcggcta tccgatgagc gaatgggaaa
    24601 atctggaagc taaactgaac gaaatgccgt tggctaaaaa agacgcacgt acttttgtgc
    24661 gattcttcta ttcggctgcc actgaatgcg aacttgataa acaaggacga atcaatatac
    24721 ccagcacgtt aagaaattat gcagctttga caaaaggttg cgtagtcatc ggtgtatcta
    24781 accgaattga aatttgggat gagacacgat ggcaagattt ttctgcagca gcagaagaga
    24841 actttgacga aattgctgaa agtatgattg atttcggact ataagaatag gaagaggctg
    24901 actaatgact gaaacatttc aacactatac agtcatgttg aaagaaacgg tagatggatt
    24961 gaacatcaaa ccggatggcg tttatgtaga ctgtacactc ggcggtgccg gacatagtga
    25021 atatttatta agccaactaa gttctaaagg gcatctgtat gcgtttgacc aagatcaaaa
    25081 agcaatcgat cacgccaagc tgcgtttagc ttcttatgtc gaacaaggta tggtcacatt
    25141 tatcaaagcg aacttcagag aattagaaga acgcttgagt gagcatgtag ataaggtaga
    25201 cggcattttg tatgatctag gggtttcctc tcctcagcta gatgaagcag aacgtggatt
    25261 tagttatcat caagatgcac cgttggatat gcgcatggat cagtcagctc ctttatccgc
    25321 ttatcacgta attaatgaat attcctacca tgaattagta aagattttct tccgctatgg
    25381 cgaggagaaa ttttccaaac aaattgctcg tgaaatcgaa cgagtacgaa aaacaaaacc
    25441 aatccaaaca actggtgagc ttgtcgaaat cattaaacaa gtcatcccag ctcccgcaag
    25501 aagaaaaggc gggcatcctg caaaaagaat ttttcaagct gtgcggattg cagtaaatga
    25561 cgaactcggt gccgaagagg cctctttaga acaggcaatc cggttattga aagtcaatgg
    25621 aagaatcagc gtaatcacat tccattcatt ggaagacaga attgtcaaaa gtatgtttaa
    25681 agaatatagt acggtacaag atctaccacc gggacttcct gtcatacctg acgaattcca
    25741 accagaactg aaagtcatta ctcgtaaacc aatccttcct tcagaggaag agctaacaga
    25801 aaacaatcga tcacgcagtg caaagttacg tattgcagaa aaaataagaa tgaaagagga
    25861 gtagaatgat ggcagaatta aagaaaatgc aagattacga atatgattta cctgaattgg
    25921 tggatcagcc ggaacaacaa atgcctgatt cttcttcgaa tcaagaaatc attcttccac
    25981 aatcaccggc aaaacgatta aagcagattt ctgctttgga aaaaattgct gtggcaagcg
    26041 tggcgattgc tattgttgct ttatgtgttt taacagttat gctgcgcaca aatatcagcg
    26101 gagctgaaaa agaaatcacg tcaatacaaa cagaaatcaa tgataaaaag caagaaaaaa
    26161 cgagtttaga acaagaaaaa aacgaacttt ctcgtactga acggattaaa caaatcgcag
    26221 aagaaaaagg attgtctatt aatgacgaca atttaaggaa agtgaagtaa atgagtctta
    26281 aaaataaaat tcggcggttt atggaaaaga aaaatctaaa tccgatgaac aatcgcaaaa
    26341 aagtaggtat cattttattt gctacgagta ttggattgtt ctttttattt gccgtccgtt
    26401 tctcgtatat cgttattgga ggtcatgttg ctggtacctc attagctgaa aaaacaaaac
    26461 agttgtacca aggaagtgaa gtagtaaaag caaaaagagg aacgatttat gatcgtaacg
    26521 gtgtggcgct cgcagaagat gcctcctctt attcaattaa agcgatactc tcaaaaacat
    26581 atacctctgg ggataaaaaa ctgtatgttg aagaaaagaa ttttgataaa atagcagaaa
    26641 tcctgcataa gaatctttct atagacaaaa aagatgcact gaatatttta gaggacggcg
    26701 caaaaaaaga gctgtatcaa gttgaatttg gtagctatgg gaaaaatatc tcccaagaaa
    26761 ccaagcaaaa catcgaagcg gacatgaaaa aagaaggtgt cgctggtctt tattttgtcg
    26821 accatcaggc tcggatgtat ccaaatggcg tcttttcttc tcattttatt ggatacgcag
    26881 ttccagacaa agatgagaat ggcttagttg gtaaactagg tttggagtct gcttacaatg
    26941 atatcttgag cggtaaagat ggtaagatta tttatcaaaa agataatttt caaaatcctc
    27001 tgccaggtac agtggcagaa gaagaaaaag ctgtcgacgg acaagatatc tacacgactt
    27061 tagacagcag actacaaagt tatttagaaa cactgatgga tcaagtaaat gaagaatatc
    27121 agccagaaga attgacagca gtcctgatga aagcaaaaac cggagaaatc ttggcaatgg
    27181 gacaacgacc tactttcaat cctgaaacaa tggaaggatt gactggaaaa gatgctatct
    27241 ggcgtaattt cttggtacaa gatagttatg aaccaggatc aaccatgaaa gttttcacta
    27301 ctgcagctgc tatcgaagaa ggagaattca acgagaatga aaccttccaa tctggaaaaa
    27361 tccaagtaga agatgctacg atcaatgacc atgactttgg tgaaaaaggt gtgctgacca
    27421 tgcgtcaagc tctttcatgg tcaagtaacg tcgggatggt catactggaa cagcgattag
    27481 gcggacgatg gtataactat ttacaaaaac tgggatttgg acaaagtact cattctggat
    27541 tagatgatga agtaaacggt gctttaccta cattaaatat tgtcgaccga gcaatgagtg
    27601 cttatggaca agcggtaggt gtgacgaatt tccaaatgat gaaagctttc acttctattg
    27661 cgaataacgg tacaatgatt caacctcgat atatcagcaa agtggtggat ccccaaactg
    27721 gagaagagcg aacaactcaa acagaagttc tagggcaacc tttttcaaaa gaaacaacag
    27781 aaaaagtccg tgaatatatg agagatgttg tcgaaagcga aaactacggt agtgcttatg
    27841 gtgtatatag tgtacctgga tacaatgtat cggcaaaaaa cggaacagcc caaatcgctt
    27901 ctgatacagg gggatatcaa acaggagata cagcctacct gtattcgatt gtggagatgg
    27961 ttccttctga agatccagac tatgtcttgt atctaacaat gaaacaccct aagacatatg
    28021 atcgaatggc attggctaaa atcgcaaatc cattgatgaa acgggcaatg gattttaaag
    28081 aaactgaaga agattcagat acagaaacaa aaacagaaaa agtatccgta gccgattatc
    28141 gtaatcttga agcggacgtt gcagcagctg atgctcaaaa aagtggactg caacctgtag
    28201 tgatcggtaa tggtaaaaaa gtacaaaaac aatctactgc caacggtgat cagttgatct
    28261 ctggagaaaa actgatccta tatactggtg gtgataagct gatgcctgac gtaacaggat
    28321 ggtcaaaagc agatatcatg aagctgggga aaatcctcgg tattgaagtt agttttgatg
    28381 gagatggtta ttgtgtcaaa caagaacttg ctccatatga aaaaatcact gaagacaaac
    28441 ttagttttac tttagaagaa taagaagaga aataggagag aaaagagcat ggaatggaca
    28501 caagcattga ttcccattgt cagcagttgt gcaatgacaa ttgccgcaat gccgctattt
    28561 attggttatt ttcaaatgaa aaagcaaggt caggcgattc gtgaggaggg accaaagtgg
    28621 cacaatagca aagccggaac tcctacaatg ggcggcttag tgtttctgat cggctctatt
    28681 ttgacaggaa tttgggttgg agcttggcaa aaacagctaa ctcctactct tttcatcttg
    28741 ctttttgtat tagcgttata cggagtaatc ggctttcttg atgattttat aaaaatattc
    28801 aagaaaagaa acatgggact gaactcgaag cagaaattga ttggacagat tctaggggga
    28861 atcatctttt atcttgttta ccgttctgaa ggttatccag gtgtattgaa tttcttcgga
    28921 ctagatctac ctttaggaat catttatggt attttcgctc tcttctggct tgttggattt
    28981 tcaaatgcag taaatctgac agatggcatc gatggattag ttgctggctt aggaagtatc
    29041 tcttttgcag cttacggtct aattgcttgg catcagcatc agtatgatgt attaatcatt
    29101 tgcttaagtg ttttaggagg tttactgggt ttcttcgtct acaacagaaa acctgccaag
    29161 atttttatgg gagatgtcgg ttcattagcg ttgggcggac ttttagcggc aatttctata
    29221 atgctaaacc aagaatggac attgcttttg attggtttga tctatgttat ggaaacagca
    29281 agtgtgatgc ttcaagttac ttcctttaag cttacaggaa aacgaatttt taaaatgtct
    29341 ccaattcatc atcactttga aatgtgcgga tggtcggagt ggaagattga tacagttttt
    29401 tggttgatca gcattgtgac ttcgttgatc acattatgga tcgtttggta ggagatgacg
    29461 ggaaatgaaa aaaatatcaa catatgaaaa taagaaagtg cttgtcttag gactcgcaaa
    29521 aagcggagta agtgcggcca aattattaca tgaattgggc gcacttgtca ccgttaatga
    29581 tggaaaacct tttgacgaaa atccggaagc acaagagctg ctttctttag ggataaaagt
    29641 aataacaggt agtcatccaa tcgaattgtt ggatgaagaa ttttcattga tggtcaaaaa
    29701 tccagggatt ccctacagtc atcctttagt agcaaaagcc caagaaatgg gtattccggt
    29761 aattacggaa gttgagctag cttacgaagt agcggaatgc ccaattattg ggattacagg
    29821 aacgaacgga aaaacaacga caacaacgat gacaggacta ctactgaatg caggagcaga
    29881 tcaaggaatt gcacgtttag cgggtaatat tgggtatcca gcaagtggtg tagcgcaaga
    29941 agcaaaatca gaagataaaa ttgtgatgga attgtctagc ttccaactca tgggaatcac
    30001 agatttccgc cctcatatag cggtgattac caatatctat gaagcacata ttgattatca
    30061 tggtacaaga aaagaatatg tcaaagctaa atggaacctt caaaaaaaca tgacagaaaa
    30121 agattatctg atccttaact ggaatcagtc agaactacaa gaattagcgc aaagaacaaa
    30181 agcacgcgta ctgcctttct cgacaaaaga agtgttggaa gacggtgtgt atgctgacga
    30241 ttactcaata tactataaaa aagaaaaaat aatggaaata tctgaacttg gcgtaccagg
    30301 taagcataat gtagaaaatg cactagctgc tatttctgta gcaaaattgt atggtatatc
    30361 aaatgaagct atacgagaaa cgttgcattt tttccatggc gtaccacatc gtacgcaata
    30421 tgtaggagaa atccaaggca gaaaatttta taatgattca aaagcaacca atattttagc
    30481 gactaaaatg gctctaagtg gatttgaaac gtctaaggtc gtgttattgg ctggcggatt
    30541 agacagagga aatacatttg atgaattaat tccttcatta aaaggaatca aagcaatggt
    30601 cgtatttggc caaacaaaag aaaagttgat ggatgctgga aaaaaagcag gcatcgagac
    30661 aatagtaaca gccgattctg tagaacaagc agttccttta gcattagaaa acagtacaga
    30721 tggtgatgta gttttattat caccagcgaa tgccagctgg gatcaatacc caaattttga
    30781 aactagagga aacagattca tggaagctgt gaaccgatta aaatagaaaa agtagaagcg
    30841 agcgataaaa aatgaaaatt ttggttacag gcggaggaac agggggacat atttatccag
    30901 ctcttgcctt tgtcaattat gtaaagactc aagaaccgaa tgctgaattc atgtatgtgg
    30961 gagcaaaacg aggtctagaa aacaaaatcg tccctgatac aggaatgcct ttccatacct
    31021 tggagattca aggatttaaa cgaaaaattt ccatgcataa catcaagacg gtccgattgt
    31081 ttcttaagag tatccgtcaa gcaaaaaaaa tcctcagtga ttttcaacca gatatcgtta
    31141 tcggtactgg aggatatgtt tccggagcgg tagtgtacgc tgcagccaaa atgggtattc
    31201 caacagttat ccatgaacaa aacagtgtac caggtgttac caataaattt ttaagccgat
    31261 atgtggatcg gatcgctctt tcatttaaag atgcggcttc cttttttcct gaaaacaaag
    31321 cagttcttgt aggtaatcct cgagcacaag aagtagcaga tactaaaaaa tcagatattt
    31381 tgaaaacatt tgatttggat ccagaaaaga aaacagtctt gatttttggc ggtagtcaag
    31441 gagcacttaa aatcaatcaa gcagtatcag tttttttaag tgaatatatt ccttcttctt
    31501 accaagtttt gtatgcttca ggagatagat attatgaaga gatcctaaat aaaataaatc
    31561 ctgaaataca aaatattagt gtccagcctt atatcaaaaa tatggcagag gtcatggctt
    31621 ccagtgatct gctggtgggg cgtgctggtg caacttcgat cgcagaattc acagctctgg
    31681 ggttaccggc agtgttggtt cccagcccat atgttacgaa tgatcatcag actaaaaatg
    31741 caatgagtct tgttcacgca ggagcagcaa aaatgattgc agataatgaa ctaacaggtg
    31801 aatcactaag tcagacagtc aacgaaatca tgggtgatga agaattgcaa aagcaaatgt
    31861 gccgagcttc taaagaacaa ggaatccctg atgcttcaaa acgtctttat gacttagtaa
    31921 aacaaataat caaaaaataa aggcggtggt cttagtgagt aagaaaaaag aaccaaaaaa
    31981 attgtctcat ccgtcttctg atgaaaacaa tttgactcct tggcaaaagg ctaaccgaaa
    32041 atatctggca gaacaagaga tgaaaaacca agagccaaaa aaagaagaca taacattaga
    32101 tacacccctt tcagattctg aagaagaaaa aacggttcaa gaggaacagc aagaagaaag
    32161 ccagtccgag aaaaaaatcg aattttttga agagatcaag caaaagaaaa acggtggacc
    32221 atataacggt tcttttttga accggctgcc caatttaaaa agtcaacggc acaaagtact
    32281 atttcgtcgg ttagctttga tcatcactgt attgggtata ccgctgattt ttttgatcta
    32341 ttatgtttct ccttatagca aattacaagc agttgcagta tcaggcaata agacagtcaa
    32401 cagccaagaa atcatctctg atacaaaatt gtcattagga gaaaatgttt ggggacagta
    32461 ttttcatcga tctacttata tcgatcgcct taaaaaagca cagccaagaa tcgaaacagc
    32521 aaatatacat ttcaaaggga tgaatgaatt cgatttggat gtaacagaat ataaagagat
    32581 tgctttgatc gcaaaaaaca atcaatacta cccagtgatt gaaaatggaa cagtattaga
    32641 tgaaaaagta gcaaatccta caaaaaatct ccctatcttg gaagagttca aagactctgc
    32701 aaaaatcaaa gaattaacaa agcaatacaa tcagttatca tcagaattac aaaaagcaat
    32761 ttctgaaatc aagtacacgc caagagcaag caataaaaat ctgatccaat tgaatatgaa
    32821 tgacggcaat caagttattg taaatatcaa taatcttgcg aaccaaatga aatattattc
    32881 gcaagtagcc aaagatatgg atgaaaaagg tgtgatcgac atggaagttg gtatcttctc
    32941 gcatccttac ggtagcactc aagaaacgga agaatcgggc caatcggaag aaacaactga
    33001 aagtgaagaa aatgcggatc aaacaaacga atcaagtgaa gaggcagaaa cgcaaaaagc
    33061 acaaacacct ggatcgtcta gcacaaacgg atcatctgat tcaaatgatt caagtgaaac
    33121 aagtcaaata gaagataatc caagtacaac tccgtcaagt ttaaatgaaa attgataaaa
    33181 caactttaaa aagtaagaat gtactttcta aaaaccagta atttatggta gaattggaag
    33241 cagtgaatat atctattaat aagtatattt taaaacggac gctagttgtt cgcttgtata
    33301 tggaatagta agtaggaggg aatcccattc atggcaaaaa caggaatgta tgtaggcctt
    33361 gatattggaa cgacatctgt taaagttgtc gtggctgaat atatcgacag ccaaatgaat
    33421 attattggag taggaaacgc aaaatcagaa gggattaacc gaggcattat cgttgatatc
    33481 gataaaacgg tccaagcgat acaacgagca gtacgacaag cagaagaaaa agcaggtatc
    33541 cagatcaaag gcgtaagtgt cggtttgcca gcaaacatgc ttgaagtaga aaattgccag
    33601 ggaatgattg cagttaacgg agattcaaag gaaatcaccg atgaagatgt cagaaatgtg
    33661 gcttcagctg cccttgttcg ttcaatacct cctgaacgtc aaatcgtgtc aatattgcca
    33721 caagatttta ctgtcgatgg gttcgaggga atcaaagatc ctcgtggaat gatcggcgta
    33781 agattagaaa tgtacggtct gttgtttaca ggacctaaaa cgatcatcca taacatacgt
    33841 aaatgcgtgg aaaatgccgg attaatcgtc aacgaaatgg tgattactcc tttagcgctg
    33901 actgaatcaa ttctttctga cggagaaaaa gattttggta cgatcgtcat tgatatgggt
    33961 ggcggccaaa caacaacggc tgttatgcat gataagcaat tgaagtttac caatcttgac
    34021 caagaaggtg gcgaatttgt cactaaagat atctctattg tattaaatac gtcatttaac
    34081 aatgcagaag cgttgaaaat aaactacggg gatgcatacc cagaacggac atcaccagat
    34141 gaagaatttc cagtagatgt gattggtcag tcagaaccag tgaaagtcga tgaacgctac
    34201 ttgtcagaaa ttatttcagc tcgaatggaa cagattttca acaaagcaaa agatgcattg
    34261 gaccaaatcg aagcattaga gttgccaggt ggcgtcgtat tgacaggcgg agctgctagt
    34321 cttcctggtg ttgtggactt ggcacaagaa atcttcggcg taaatgtaaa attgtatgtt
    34381 ccaaatcaca tggggctgag aaacccggta tttacaaatg taataagtat cgtggattat
    34441 tcagcaaact tgagtgaagt gtatcaattg gcaaaaattg ctgttacggg tgaaacctca
    34501 gcagctcgtc aaatggtagt tgaacaagaa acaacaaaca catatgagag ctatgaagca
    34561 cctgaagaaa caatctacga tgagccggaa ccaaaagagt ccggagaaaa tgtaaaaaat
    34621 aaaatcaagg gtttctttac aaatattttt gattaattca tcctgggagg aataagatat
    34681 ggaattctca attgataata atatcaatga cggcgcagta atcaaagtca tcggagtcgg
    34741 tggtggcggt ggaaatgctg taaaccgaat gattgaagaa aacgttaaag gtgttgaatt
    34801 cattacagcc aacacagacg tgcaagcatt aaaaaattca aaagctgaaa cagttatcca
    34861 attaggcccc aaatatactc gtggtttagg tgctggttct cagccagaag ttggacaaaa
    34921 agcggcagaa gaaagtgaac aatccttacg cgaagcttta gacggtgcag atatgatttt
    34981 catcactgca ggaatgggtg gaggaaccgg tacaggtgct gcaccaatcg ttgcaggtat
    35041 cgcgaaagaa ttgggcgcat tgactgttgg tgtcgtgaca cgtccattca cttttgaagg
    35101 accgaaacgt ggccgttttg ctgcagaagg tatcgctcgt ttgaaagaaa atgtggatac
    35161 attattaatt atctcaaata accgtttatt agaagttgta gataaaaaga caccgatgct
    35221 tgaagctttc cgtgaagcag ataatgtttt acgacaaggt gtacaaggta tctctgattt
    35281 gatcacagcg ccaggttacg ttaaccttga ctttgctgac gtgaaaacag tgatggaaaa
    35341 ccaaggtaca gcattgatgg ggatcggtgt ggctagtgga gaagatcgcg tagtagaagc
    35401 aacgaaaaaa gcaatctcct ctccattgct tgaaacatct attgatggag ctgaacaagt
    35461 acttcttaac atcacaggcg gtttggatat gacattgttc gaagcacaag atgcttcaga
    35521 tatcgtagca aatgcagcaa ctggcgatgt aaatatcatt ctaggtacat ctatcaatga
    35581 agaaatgggc gatgaaatcc gtgtgacggt catcgcaaca ggtatcgatg aatcaaaaaa
    35641 ggaacgcaag tctagccgac cagctagaca agcacaaatg caatcacctg ctcaaaagac
    35701 tgtattagat atggatcaag ctaaaccaac ttcttcagaa gaagaaaaca gttttggcga
    35761 ttgggatatc agacgtgaac aaaatgttcg tcctagagtt gatgattcca attttgacaa
    35821 tatcgaaaaa aaagaatttg atacattcaa ccgcgaagaa acaaaatctc atggcgatga
    35881 tgaattaaat acgccaccgt ttttccgtcg taaaaaataa aggagaggaa gagcacatga
    35941 ttgctgacaa cttgcagaaa atcaagcaag aaattcagga ttcgtgtgct cttgttcatc
    36001 gtgatccgaa agaagttaca ttagtagctg taacaaaatc tgttgaatcc gctcaagctg
    36061 aagaattggt tagagctggc gttatgaatt gtgcagaaaa ccgagtagat aaattattag
    36121 aaaagaaaca agatttattt gattttcctg aaataaagtg gcatttaatt ggtaatttac
    36181 agcgcagaaa ggtaaaattg attgtaaatg aaattgatta ctttcatgcg ttggacagtt
    36241 taagattagc agaagaaatc cagaagcggt tagagaaaga attatcctgt tttattgaag
    36301 taaatgtttc tggtgaggaa tcaaaacatg gaatttcacc agatgagctt ttaccgtttg
    36361 tggaatcttt agctgcctgc acaaaaatca aggttgttgg gttgatgact atggcgccta
    36421 aagaagcttc tgatgagcaa atccataaag tgtttggtac gctgaaagaa ttgcgtgatc
    36481 ttgttcaagc aaaaggttat gcccatgcac catgtagtga attaagtatg gggatgagtc
    36541 aagactttca aattgcgatt gaagaaggtg caacatttat ccgtgtagga acagcgctat
    36601 tcaaagacga ataaaggagg aacaaaaatg ccgcttttaa acagaggagc aattgctaat
    36661 tttttcggat tagctgatga ggaagagtac gaataccaag attatcctga aacaacaaaa
    36721 aaggctgtac agccaactaa aaaaaataca acacagaatc aatcaactca ttctgtatct
    36781 gctgcgaata ctcatgaaaa acaaaaaacg gcgacttcac gtcctgtaac gcatgaaagt
    36841 attcagccgg ctgaaaagaa aaaagtaatt gaactacatc aatcacctac aaaaactgta
    36901 gcgaaagaaa gaacagcttc aagaacctct acacaaccta aagcaaatct tgctggaaaa
    36961 atcactgtaa tggaaccaag agcttattct gaagctatgg cgattgctaa acgaatcatt
    37021 gctggagaag caaccttagt gaattttcat ttagtagaag aaaaacaagc gagaagaatc
    37081 gttgattttc taacaggaac agtttatgca ctagatggag atatcaaacg tgttggagat
    37141 gagatttttc tttgtacacc gactgggatg gagattgaca gttcaaccgc tcaatctttt
    37201 gcagatgcga atttgtttga tctgtaagag gaggaactac gatttatact attgcactat
    37261 tacttcattt acttcaacgg gcagcctatt tctatagcat tatcttgatg gtatatgcat
    37321 tgctctcttg gtttccaggc gggtatcaat cagggctagg acgattatta gctaagatct
    37381 gcgaaccgta tatcagtttg ttcgatcgtc tgcctttgag gattggaccc ttggatttca
    37441 caattttagg ggcaatcctg atcttgaact tagccgtaca aggtttagca cagatcgtta
    37501 tatggataac tgtccattta gtttattgat tgaagcaaca aaatctttat tctcagaata
    37561 gataggaggg gaaaattttg gatgcaaatg tataccagca ctttcgggca gatgaacgta
    37621 catttatcga ttcggtaggc gattggattg aacaggttca aggacaatat gcaccctatc
    37681 tgaccgaatt tcttgaccca agacaatcct atattttaga aacactgatt cgacaaagca
    37741 gtgatttgcg tttcatgttt tatggcggat atgaacaggc agaaagaaaa agatgtttga
    37801 ttttccccga ttattacgaa cctgtagagg aagattttga aacagcattg tatgaaatcc
    37861 attatcctag caaatttgcc agcctaagtc atgggaaaat ccttggaact ttagtgggaa
    37921 cggggatcaa acgctcttac tttggcgata tcatctcgga cggtgatcgc tggcaagtat
    37981 ttattgcaaa tgaggtggat cattttgttg ctgcacaagt aacaaagatt ggaaaggttg
    38041 ctgtacgctt ggaaccaatc aaatacacag aaatgattat gccgaaagat agctggacac
    38101 aagaaagagg aacagttagt tccatgcgac tagacagtgt gatctctgag atttataata
    38161 tttccagaca gcggtccaag cagctgatcg aagctggtaa agtaaaagtg aactggacag
    38221 aaaatacaag acctgatttt gtacttgaac tattagatat tatttcagtc agaggttatg
    38281 gcagagtcca gatccaagaa cttgagggga agacgaaaaa agaaaagtac agggtactgt
    38341 tcggtgtatt acgtaaataa aataaaaagg tggtttactc atggctttaa ctccattaga
    38401 tatccaaaat aagacattcc cgacaaaaat gcggggatat aatcaagacg aagtagatga
    38461 tttcttagat ttagttgttc gtgactatga ggaattgact caaagaaatc gagaattaga
    38521 aaaagcagtc aaacattctg aagaaaaact tgagtacttc aatgaattaa aagatgcttt
    38581 gaaccaatca atcatcgtag ctcaagatac ggctgataaa gtaaaaacaa gtgcaagcaa
    38641 ggaatccgaa gtgatcgtta cttctgcaca aaacaaagca gatgagttag ttgcgaacgc
    38701 tgaaaaacgt gcacatcaat taacaactga tgcagaagaa aaagcaagaa aaatcttgac
    38761 agatgcaaca gaaaaagcaa gacagcttgc gacagaaact gaagatctta agaaaaaaac
    38821 acgagtattc catcaacgta tcagcttgat gcttgagtct caattagaac aagtgaaaag
    38881 tccggaatgg gatgaaattt tacaaccatt ctccagctac gtgactgaca gccatgaagt
    38941 gatcaaagaa gttttagcaa aacagcttga caatgaaaat gatactgatg taaactcaga
    39001 cacaggagtc atagaagcac ctatgacggc atttgataca caggctattg atatctcgat
    39061 cgtgcctggt gaaaatgaaa acgagtagaa cagaaaagca ctttgttatt cttaaagcga
    39121 gccgatgaga gtggaagttc ggtaagacac tgcatggtga aagatcctct acttgagacc
    39181 tgcttttccg aaattagagt aagaagcagc ggctgacctc gttattggtc acagagagtt
    39241 tgcactagat agatgcaact aaatttgggt ggtaccacgg catgttcgtc ccttgcggct
    39301 gaacatgctt ttttgtgtcc ggaaaacaaa taaaggagtg tatggttgaa tgaaaatgaa
    39361 agaaaccttg caattaggaa aaacaaaatt ccctatgcgt ggtaatttgc caaaccgaga
    39421 aggcgagtgg caaaaagaat gggaagaaaa tcgaatctac caaaaaagac aagagttgaa
    39481 cgaaggaaaa cctagctttg ttcttcatga tggacctcca tatgccaatg gtaatatcca
    39541 tttaggacat tcattgaata aaatcagtaa agacattatc attcgttcaa aatcaatgtc
    39601 tggctatcgt gcaccatatg ttccaggatg ggatacacat ggattgccga ttgaacaagt
    39661 attggctaat aaaggcgtga aacgtaaaga aatgtccatg gcagaatact tggaaaaatg
    39721 tcgtgaatat gcactttccc aagtagataa gcaacgagca gatttcaaac gcctaggagt
    39781 tgcaggagat tgggaacatc cttatgttac attagatcct tcttatgaag ctgcacaagt
    39841 acgcgtattt ggtaaaatgg ctgaaaaagg ctatatttac aaaggattga aaccaattta
    39901 ctggtctccg tcaagtgagt cttctttagc ggaagctgaa attgaatata aagatgtaaa
    39961 atctccatct atttttgtag ctttcaatgt caaagacggc aaagatattc ttgatgaaga
    40021 tacagctttt gtcatttgga cgacaacacc atggacattg ccagcaaacc aaggtattgc
    40081 tgtaaatcca aaatttacgt atgtattggt ggaagccgat ggccgtaagt ttgtcgtagc
    40141 gaaagatttg ctagaaacag tccaatcaga aatcggttgg gaaaatgtaa cagttctaaa
    40201 agaatttgct ggacaagaaa tggaatatat gactgcaagt catccattct atgaacgtga
    40261 atcacttgtg attttaggcg atcatgttac tttagacgct ggtactggat tagtccatac
    40321 tgctccagga catggggaag atgactatat cgctggtagc aaatataaac tatctgtagt
    40381 gtcacctgta gactcaaaag gattgtttac agaagaagca cctggatttg aaggtatctt
    40441 ttatgacaaa gcaaatccta tgatcactga tctattaaaa gaaaaaggag ctttgctgaa
    40501 gctagatttc tttactcata gttatcctca cgattggcgg acgaaaaagc ctgtgatcta
    40561 tcgggcaact ccacaatggt ttgcatctat cgataaattc cgtcagaata ttttggatga
    40621 agtagaaaaa gtagagtggg ttatcccatg gggtaaaaca cgtttgtata atatgatccg
    40681 tgaccgcggc gattgggtaa tttcccgtca gcgtgcatgg ggagtaccac ttccgatttt
    40741 ctatgctgaa aatggagaag caatcatcac accagaaaca atcgaacatg tggcaaaatt
    40801 atttgctgaa catggatcaa ttatctggtt tgaaaaagaa gcgaaagaac ttcttcctga
    40861 aggatttacc cacccaggtt ctcctaatgg cgaatttaca aaagaaaaag atatcatgga
    40921 tgtctggttt gattctggtt cttctcatga agctgtttta cgtcagcgac cagaattatc
    40981 attcccagca gatatgtatt tagaaggatc agaccaatat cgtggctggt tcaactcaag
    41041 tatcacgact agtgtggcta tcaatggtgt agcaccatac aaatcagtta tttctcaagg
    41101 atttgcttta gatggtgaag gccgtaagat gagtaaatct ttaggaaaca tcattgcacc
    41161 agaaaaagtg atcaaacaat ttggtgcaga tatccttcgc ttatgggtat ctagtgtaga
    41221 ttatgaagca gatgttcgtg tttctatgga tatcttaagt caagtggcag aagtatatcg
    41281 taaaatcaga aatacgatgc gtttcttatt agcgaataca gaagatttcg atcctaaaaa
    41341 agatactgtt tcgtacaatg atttacgttc tgtagacaaa tatatgaccg ttcgattgaa
    41401 ccaagtaatc aaagaaatcc gagaagaagg gtacgacaaa tataacttca tgcatattta
    41461 ccgtacagtt atgaacttct tgacggttga tctatcgtca ttctacctgg attttgctaa
    41521 agatgtagtt tatattgaag cagaagatga ttatcaacgt cgttgcatgc aaactgtttt
    41581 ctatcaaaca gctgtagctt tgacaaaatt attgactcct atcattccgc atacagcaga
    41641 agaaatctgg tcattcctga aagaagaaga ggagtacgtt cagttaagcg agtttcctgg
    41701 ttatgaagag tttgcaaacc aagaagaatt gatggatacc tggactgctt tcatggactt
    41761 ccgtgataat gtcttaaaag cattggaaga agcaagaaac agcaaattga ttggtaaatc
    41821 tttggaagca aaactaactg tttatccaaa acaacaagtt cgtgaaatgt taaaagcttt
    41881 ggatgcagat attgcacaat tattgatcgt ttcaccagac tactttgaag ttatggcagc
    41941 agatgaacaa gtgccagata acgctatggt atttgatgat gttgctatta cagtggaaaa
    42001 agcagacggg gaaacttgcg atcgctgtcg ccaagttcgt aaagatgtag gagtagatga
    42061 aaaacttcct catctatgcg gtcgttgtgc aaaaatcgtt gaagacttct atcctgaagc
    42121 tgtagctgaa ggattcgaag aaaagtaaaa gggaaaaggg ggtgtgacat gttctttgtc
    42181 acaccccctt ttgttatcaa tcgtttaatt ttccacgttc acgataccag ttatctggtt
    42241 gccagatcca ttcgaatccg tctttttcaa gtagttcgaa agctgcttta ggtcccattg
    42301 atccagcttt gtagttggga aaatccggtt gtgtttggtc ccaagctttt cgaatgacat
    42361 ccacgatatg ccaagattga gcgacttctt cccaatgaga gaagttcgtt ccatcaccat
    42421 tcaacgcatc taatagtaat ttttcgtaag cttctgggct attttcaacc atttctgcac
    42481 tatttctaaa ttccaatttg attggttccg tttcaaatcc ttgccctact tctttcccat
    42541 tcaaggataa agaaaatcct tcagttggct gaatatagat cgttagaaca ttaggtggaa
    42601 gtgtcgtatc gtcacatggt tcatcgacag atgttttgaa tacattaaca ggaacttgtt
    42661 tgaatacgat gttgatccga gtcccttttt cagtcaaacg tttgcctgta cgtacataga
    42721 aaggaacacc tgaccaacgg aagttatcga tagtgaattt gcctgcgacg aatgtttcag
    42781 tctctgattg agcatccact ttgtcttctt cgcgatagcc cttgaattgt acaccatcca
    42841 attcaccttg agcatattgc ccgcgaacga agttttctaa cacttcttct tcgttataaa
    42901 tgcggatagc tttcaatgct ttgatctttt ctgtacggat ttctttttcg gaaaatgcta
    42961 ctggcggttc cattgcaagt aatgctacaa cctgtaaaat atgattttgc accatgtctt
    43021 taagtgcgcc actatgatcg tagtagcccc cgcgatcttc tacaccgagg ttttcgccaa
    43081 aagtaatttg gatattgtcg atatagcgat tgttccattg agcctcaaaa atattattgg
    43141 caaaacggat agctgagatg ttttggatca tttcttttcc taaataatga tcaatcctga
    43201 aaatatcttg ttctgggaat acttctctga tttgctggtt caattcaaaa gcagactggt
    43261 aatcagaacc gaacggtttt tctatgatca aacgattgaa gccatttttt gtcataatat
    43321 tttgtgattt caaatgagaa acgatcgttc caaaaaattg aggtgccata gccaaataat
    43381 agacacggtt accgtttaat tgatagtgtc catctaatcg gtcagacaat tcttttaatg
    43441 tctgataatg cgttgaatca tttacatcat gggattgata gtaaaaatgg cttgcaaagg
    43501 tagttgcttc ttcatctgaa ggattcaatg cacggattgt ttctttgaca acttcacggt
    43561 agtgctcatc gctccaggga cggcgagcgg ttccgattac agcaaaatgc tcgcctaaat
    43621 ttccttttct gtaaaggcgg aacaaagacg ggtacaattt acgctgggct aggtcacctg
    43681 taccgccgaa tatcgtaaac aatacatttt tttcattaga catgtggata cactccttta
    43741 tattttatcg acaaaaatcg tgctggccgc cttaaagctg acggggattt tctttctatc
    43801 attttggata gtgatcggtc cttcataagc tgcgatgtca acgatctcat attcttcgtt
    43861 gatattcagg tcaatagtga ctaagtaatc caacaattct ttttcatcaa gcactcgtgc
    43921 gatacgcact tttgtaccga caggatagga ctccaatgtt tgtcggcgtt cttcatggac
    43981 aacttcctct tttttaggaa tcgatcctcc atgaggacaa tgttcaggat gattcaaata
    44041 gttttctaaa tgatctgcaa gatgttccga agtcacatgt tccaatactt cagcatcatc
    44101 atggacttca ttccagctat agtctaaatg ttcaactaaa aaaacttccc ataagcgatg
    44161 tttgcgaatc agactgctag ctttttgcag tccgcttgca gtcaattgta caccttgata
    44221 aggggaatgt tctacaagct cttctttcac aagtttagaa atcatttcac tgacagatgc
    44281 agctgagaca tctagactag atacgatttg tttattgttg actttcgttg tatctccacc
    44341 tagttctaaa atgattttca aataatcttc tcgatttggt gtcatattcg atcatccttt
    44401 catggtaaaa aaccatcatt agtaagtgta acaaaaaaaa cgttaattga ctatttttct
    44461 aaataaaaaa caaaggattt ttagacgttt ttacaacaaa aaacgaaaga tgatagagaa
    44521 gactcattct ttcgtttata aaggatatat atatacattg ggagggatct atttttcaga
    44581 taacaactta tatgaagtgt ctgcagattc gtcataaaga gtttgttcgg gataagaaaa
    44641 gataagataa aaaccatact gatcacgtac gactaaacga tattcatcta tttctaccac
    44701 agatgtgcca agagactgac cgttgatcaa aaacgtgtaa tcagatgtaa tctgtaattg
    44761 ttgtttttgc tggctatcac taaagaacca tgtaccttga tactttgaga gtccttctga
    44821 cttttccgct tgttttttct ttttcaagta aataaaactt gagagaacgg taatgagtga
    44881 accaagaaaa tagatcattt tttttatcac caaatcactc ctttatgaga aaataatcgc
    44941 atagacatat tctttcttct ttatttcatt gtaccgaata aaagctaaaa tttctctaag
    45001 actttcgact ggttttttta ctgtttttct acaaattatg ataggataca tagtaggaag
    45061 aaaggtgaca taatttgaag tatcagcaag tggaaatagc tttttttatt gaccgcccga
    45121 atcggtttat agcacattgt atgaaaaaag acggagaaat aattattgcg catgtcaaaa
    45181 atacaggtag aggaaaagag gtcctgatac caggcgctga agtggctatt gtatttgctc
    45241 ctggacctaa acggaaaacc gcctatgatc tgattgctgt aaaaaagaag ggtgactggt
    45301 ttaatatcga tagtcaattg cctaatcggc tggcaattga tggtatttta gacggcacga
    45361 tccttttacc aggattaact ggtccattta cggtatacaa gcgtgaagta acttatcttc
    45421 actccaaatt tgatatttat ttagagacgg tggtcggaca aaaagcattt gttgaagtca
    45481 aaggtatgac acttgaaaat aaagcgattg gtgcttttcc tgatgcgccc acactcagag
    45541 gattgaaaca tataggagaa ttaacgtatg ccgcacaaga tggttatgcc gcttatgtct
    45601 tgtttattgc tcagttcgaa catcttcatc tggcaacgat ccatgaagaa atgcaaccag
    45661 cactagctga tatggttcga catgcgcagc aaagcggagt tcaaatcctc gcctataatt
    45721 gtcaagtatc ggcagatgag gtaactgtca agcaggccat tccatttgat gtagatatcc
    45781 cttttgaaga tcctatacaa tagaaagaag gtaaaataat gatcaactta ggtattatcg
    45841 gaacaaattg gattactcat caatttgtcc aagcagcttt aagcacaaaa aaatatgaat
    45901 tgacagcggt atattctaga catttggaaa aagcacagga atttggttct cattatgaag
    45961 gggatatcga attcgcaaca gatttgaaga cttttttcaa tattactcat atggacacca
    46021 tttatattgc ttctccgaat agtcttcact ttagtcaagc taagcaagca attttggcag
    46081 gaaaaaatgt gatagttgaa aagccagctt tttcgacgcc aaaagaaatg gatgaaatca
    46141 tccatttggc aaatgaacac aatgtgtatt tttttgaagc agcaagaaat atccatgaat
    46201 caggttttaa aaagattgcc gattacttac cattaaaaga cgagataatc ggtgcaaatt
    46261 tcagctatat gaaatattct tcgcgctatg accaagtatt agatggagaa gaaccgaata
    46321 tcttctcgac tcatttctca ggtggtgcat tgatggattt aggtgtttac cttgtatatg
    46381 cagctgttgc ttggtttgga atgccaaaag aagtccatta tttcccaaga aagatttcta
    46441 ctggtgtaga tggtcttgga tgggggatct tacgttatga gaattttgat gtatcgatcc
    46501 agccaggtaa aatcggtgat tcttatttac cgtcagagat ttattttgac tcaggaacgc
    46561 tagtgatgaa tggagtcaat gcaattgaac aagcacaatt tcatgatcgc gcgcataaag
    46621 aaatcattga tttggcggta acagctgcag aaaatccaat ggcagatgaa gcagaggatt
    46681 ttgccaatgt catggctcat ccgaaagatc cagcttgggg attgttgtat gaagagtgga
    46741 tcgagctagc tagaaatgtg aaccaagtga tttatgattt aagaaaaaat ggcggcatca
    46801 aattcgatgc agataatgag gaagattttt aatgaacgaa tttgtacaag tatttcccga
    46861 aaattggcag aaaaaatatg aggataaagg gtttatctca ccttctctta ttcaacgagc
    46921 agtttttcag ccactaagta ataaacaaag tatagtagcg gtttctccta caggttcagg
    46981 taaaacactg gcttatcttt ggcctctact actaaacgtg gaaccaggtg aagcaagtgc
    47041 gttggtcatt tttgcttctt ctcaagaact agctattcaa gtagcagatg ttgcacgtga
    47101 atggggaaaa gacaaagagt tgaaagtcca atctttggtg ggcggagcca acgtcaaacg
    47161 tcagattgag ggtttgaagg aaaaaccaga aatcttgatt ggtacaccag gaagaatatt
    47221 ggaattgatg aaagcaaaaa aaataaaagc acatcaagtg aaaacaatgg tatttgatga
    47281 agcagaccag ctatttgacg ctgggaacag ccaaatcatt gatcaaattc ttcatcaagc
    47341 tccaacagag tatcagctag cttttttttc agcgacagca gatcgttccc ttgaaagcat
    47401 tgaaaaaatc actgggaaaa cgtttgagac aattgacgtg acagcagagg acgattctcg
    47461 aaaaggactg agacattact ttttacgtgt accgatacga aagaaagatg actacttgag
    47521 acgtctcacg catatagaag attttcatgg tctgattttc tttaatcaac tgactgaatt
    47581 aggcgtaatg gaagaaaaat tgctgtatag aggtgtaccg gtaggaagtc tggcctctga
    47641 tcagaacaag ctactccgaa aagcagcgct agaacaattc aaaaagaaaa tggtcagagc
    47701 acttttgacc acagatgtgg cggctagagg tttagatatt acaggtcttc cttatgtagt
    47761 gaatgcagaa gttccgttaa gtgaagaagc ttatttgcat cgttcgggaa gaactggtcg
    47821 aatgggtaat gaaggaatag taattacact cgtacaagat aataatctaa gagatttaaa
    47881 acggctttta cgtccatcaa atataacact tgaagagatt tttttatacg gaggtaagct
    47941 gcaaactgag cagccaacca aagaagatgc agctcctgca gtaaataaaa agtatgcgaa
    48001 aactttttct ggaaaggagc agaacccaga atataacaaa gaaaaaataa gaaaaaaagt
    48061 taaaaataaa tcaaaaaaag agagaaataa aggagcaagg cgcaaataag cgccttttta
    48121 tgctatgatt atgaaaacgg gaacaaaatt ggttgccaga ggaggggtta tgtgaaagaa
    48181 cgtcaagaag agatcttgca gctattgaca gaaaaccaac aaggattgac agcaagtgat
    48241 gtagctgagc gcttaactat cgatcgaagc aatgcaagcc gatatttaag tgaactatac
    48301 aaggcccatc atattgtaaa aaccgctggc cgaccagtcg tttactcttt gccaacggaa
    48361 aagtcaaaat ctgatgaagt gcatgtcgac tcttctacgc aagtgacttt tgaaacactc
    48421 gttggagaaa atgattcttt aaaagtcagc atacagcagg caaaagctgc gattctttat
    48481 ccaccgcggg ggttgcatac gattattttt ggagaaactg gtacagggaa gtcgatgttt
    48541 gctgaatgta tgtatcattt tgcaatcgat tcagagatgc tttctgcaga tgcgccattt
    48601 gtttctttca attgtgcgga ttatgatcaa aatccgcaac tgctctttgg acacatcttt
    48661 gggatcaaaa aaggggctta cacaggagca gctcaagata gtcccggtct aatagccaaa
    48721 gcagacggcg gtatattgtt tttagatgag atccaccgtt taccgccgga agggcaggaa
    48781 atgctgttta ctttcattga taaaggtatt taccgtccgc ttggagaaag tgcacaagta
    48841 catgaagcat ctgttcagat cattggggcg acgactgaat cttcagaaag tttcttatct
    48901 acgttcaatc gacggattcc tatggcaatc accttgccga atcttgcttc acgttcactg
    48961 gatgagcgtt atgaaatcat ctctcttttt atcaaacaag aagcgaatcg tttgaatcaa
    49021 cgtatcgata tcgaaaaaga agcgattctt gccttcatgc tttatgatgc ggaaggaaat
    49081 atcggtcaag tgaaacgaga cttgaaacta gtctgtgcaa aatctttttt acattaccgt
    49141 actcatcatg agaaaaagct aatcattcga aaagaagaac tttcccttca agtacaaaaa
    49201 ggattactga aaatcaagga aataccggaa cgtctcgacc gattcatgga cagcaaaagt
    49261 cagtatttga catttgaacc aggatttgct gatgttgtct ggtcacaaga tccagaacga
    49321 aacatgcagg tctataacga tattgaggaa aaagtcactt ctctttccga aaccggtgtg
    49381 gagaacattg atctagagac cctcatttca aaagatgtgg atgcttattt ccagacatat
    49441 gtcaaagaat tgactaaaag ttcgattccc aaagatcttt tgccagatga aatctggcgg
    49501 ttaaccaatc aattgtatga tgtggctgaa aaacggttag accgtattta taatgaaaaa
    49561 gctcgttttg cttttgcttt gcatctgcaa agtacattgg atcgtgtgag agaaggtcat
    49621 atgatcgtcc acccagattt gaataacgta cggaaaaatt ttaagaggga attccaggta
    49681 gcaatcgatc tttcaacgat cattgaagaa gaagaacaag ttgagattcc ttttgatgaa
    49741 atcggcttta ttagtatgtt tctttctatc aatctaggag aaagtgagcc tgttaaagaa
    49801 aacaaagtag atgttctcat attgatgcac ggcagacaga ctgcttcaag catgttggag
    49861 atggcacaag aactattagg aacaaaagta gggaaagctt ttaatatgcc actggttatg
    49921 gaagttcaaa caatgtatga agaagtgtta caatatgtca aaaactatca agagcatttg
    49981 acgaatggcc tactcttgtt aacagatatg ggatcattaa acaccttcag tgatttgatt
    50041 tatgaagaga ctggcattcg gacgaaagct atcacgatga caagtaccat gattgtgata
    50101 gaagcgatac ggatggcaag tgttggaaga agcttagaag atatttatca aaatatccag
    50161 ctttcctttg aatcgatcgt acgtgatcag ttcaaagcgg aaaagtctga aactttacag
    50221 cgtaaaaaag ccgtaatcgt gacatgcttt actggtgaag gagtagctgc caaattatat
    50281 cagcgtatta tgccagtgat taatcaaaat aaggtagaac tgatccagat gcagttttta
    50341 gaacgtgaat cattcaaaaa acacatcgat gccttattgg aagaatatga gatcaaagca
    50401 attgcgggaa cagttgatgt tgattatcaa aatatcccat ttttttcagc ctatgacgtt
    50461 tttgatgatg aaaaactaaa tattttaaag cgtatagctg atgatgagat cccgacagct
    50521 aaaattgtcg attcacttgc tggatcatta agcgatgtct tttctattga tgaactgatc
    50581 cgttcattgc aaaagacagt tcagcagatc cagaccgatt tgtcgattat cgtggaacct
    50641 ggagtagata ctggaatcgt tatccattta gccttcatgt tagacggatt gatcaaacaa
    50701 gaaagccatc ggcaatttga gaaactgtct attttctcaa aaatttatcg tttggaaatg
    50761 gatatcgttc gggcaaactt gatggcacta gaacgaaaat atcaggtaaa aattccagaa
    50821 gatgatatcg catttcttac acaaatgttt cttgaaaata aaataaaaaa atgaacacac
    50881 taatcatact gtttacacac taaacatctg tgtgcgcact ataaaaaaga gctaaaaagc
    50941 atgaaaacgc tatttcagct ctttttttta ttggcatgct tcttgcgtta tataaaagtg
    51001 taaaaaaata aaaggagtgt taaaaatggc aaaaaaaaca atcatgttag tttgttcagc
    51061 agggatgagt acgagtttat tggtcacaaa aatgcaaaaa gcagcagaag aaaaagggat
    51121 ggaagccgat atttttgcgg tctctgcctc agatgcagat aataatttag aatctaagaa
    51181 tgtcgatgta ttattattag gtcctcaagt acgtttcatg aaagctcaat ttgaacaaaa
    51241 acttgcgcct aaaggtattc cattagatgt catcaatatg caagactatg gcatgatgaa
    51301 cggagaaaaa gttcttgcac aagcagaaaa tctaatgaaa taaatcaagt atgcagttgg
    51361 gggtataaca acgtggaaga tcaacaaaat ctagaagcca tcatggggtt aatcatgttt
    51421 ggcggtaatg caaagagcga tgcaatggaa gcgattgcgg ctgctaaaat cggtgatttt
    51481 gagttagcgg ataggaaaat cgctgatgca gaagaatcac ttatacaagc gcatcatgca
    51541 caaacagaca tgttgacaca agaagcacaa ggaaatcata tgcaagtgac attattgact
    51601 gttcacagtc aagatcactt aatgacatct attgcgttta ctgatttagc aaaagaaatc
    51661 atcgaacttt atcgtcgcgt atacagcgaa taaatacaac ttcttttttg aacggaagaa
    51721 gaaaaatttt tgaacggatt ttttattttg ttataaggag aactctcttt atataaaaaa
    51781 cttataggag gaagtaaaaa atgaacggac tatcaagttg gatggagaaa tacatcctgc
    51841 cggttgccgc aaaaatcgga gcgcaaaaac atttagttgc tttgcgtgat gcttttatcg
    51901 gcatactgcc cgcaacaatg gcgggatcag tcgctgtaat gatcaatgca attattcgtg
    51961 atttaccgca acagtttaac agtacctatg ctgacagttt agcagcagac aaaggaatct
    52021 ttgccgtatt gaatgtcatc atcggaatca atggttttgt ctggaacggt acactagcaa
    52081 tcgccggatt gatctttgcc ttttcttggg ggtataatct tgcccgagct tatggagtaa
    52141 atgatctagc tggggggatc gttggtttag ctaccttgat tcaaggtatt tcctttggtc
    52201 cagcattatc agcagcatta gatgtgaatg ttccttcaaa tgtcgcagat gcaatcaata
    52261 gttctgagat tggtgcaacg attgcagatg gcacactgtc agttggatta tggggttggc
    52321 taaaactaga tcatttgaat ggaaacgctt attttacagt tatgatcatg ggagctattt
    52381 cagtcattat tttttgtaaa ttgatgcttg ccaacattac gatcaaaatg cctgattcag
    52441 taccaccagc agtatctaaa gcatttgcag ccattttacc agcgacgatc gctttatacg
    52501 tcattgctat tatcaattat atcgtaggtc gtgtagctga tgggcaactg atcattgatt
    52561 tggtgcaaaa atatattgca gaaccattcc taggattatc tcaagggttg ggagcagtat
    52621 tgatcgtcac agtatttgtt caagtattct ggttctttgg tattcatgga tcaaatgtat
    52681 tggcaccagt gctagaggga atctggggac aagcacaact aatcaatatt gatattttcc
    52741 aaaaaggtta taacggaaaa actggtacac cagccgtgtt agatgcaatc gaagatggaa
    52801 aagcttatat gtgggtccgc ggatcgttcg atgcttttgc atggtttggc ggatcaggcg
    52861 gaacgatcgt cttactgata gctatcttga ttttctctaa acgtgcagat tatctaacag
    52921 tagctaaatt atccattggg cctggtatct tcaatatcaa cgaaccagtg atgtttggtc
    52981 tgccaatcgt attgaatgca atcatgttta tcccattctt gattgctcca gtagtagcaa
    53041 cagcaattgg atggacagct acttatcttg gcctagtcgc tccggtatcg cagcaagtca
    53101 catgggtagt accgccgttc ttattgtcat tcttagcaac aggagcggat tggcgggcac
    53161 cgatcgtaac attagtatgt atggtcgtga ctttcttgat ttgggcacca tttgtcatct
    53221 ctgcaaacaa aatggaccct gccgcaggcg aacaacaata agatgaacgt gaggagcggg
    53281 acgctttaag tttcgttcct cataatgaat tttcgaggag gaactgaaat gattacaatt
    53341 gccagtgcaa tgcaacaaga tgaaatcaaa gaattgttag aaggatacaa tgaagaagga
    53401 atgactttta catttaaaga aaaacaagga atcaagctgt tctttgaaac aagtgcagaa
    53461 gatccggaag ttgcggctaa aaaagcaaag gaactaatca aagcacagcc ttggggaaca
    53521 gtcctttatt ttcaatcaac agctgtttag aaaatggttt aaaaagatag gatggttgag
    53581 atgataaaaa acggtttatt tttatgtagt attggttctt tgatcgtgtt gtatagtttg
    53641 aatccacatt cgcaaaggta tgatttgtat agtatggtca caggtctttt tctaacagga
    53701 ttaggaatct atttcttcat gaaggggaaa aaaagagaag aagcaaagga gaaaaataac
    53761 taatggaaat cacgaaaaaa ttaaatattc cagcatcgtt tttttacgat aaagttatgg
    53821 actctgtcct tttcgatgta cgtaaagcga ccggcaaatc agtaactaga aaacagctga
    53881 aaaatttgga gtacgtcaaa caattctcgc aaaataaccg ggctcgcata aaaatcgaag
    53941 aagtcattga aaaccagtct tataagtttc gtacatcgac aacgaagaat gaattcgtcg
    54001 ttcattatca aatcaaacca ttggacgatt cgacctgtga ggttcattac acagaaaaaa
    54061 tggagtctta tggcatgctt caaaagttga atgatatgct tttaggaaca atcttgatgt
    54121 attttaaacg gagacgtttc aaaaaaatgc tagatatgat cgaagcaacg tattaaatca
    54181 atgaaacatg gtactattta gttattgaaa aatgaagtaa gctagaagga atttttcata
    54241 ggaagataga tctttcctgc gaatcctcac tagctttttt gtaggaaggg gcagcaaaat
    54301 gacaaacaaa gtaagagagc taggtgtatc ttcgattgag gaaatgtttg atttagccac
    54361 ttatgcattt aatggggcag atacacctga aaggcgagaa cgttttcgta cacttgcgga
    54421 gaattcttgg aattacggat tttttgatga ggaggatcgg ctaacaagtc aagtcatggc
    54481 gacccctttt ccagtgaatt tttacggaca agagtaccta atggccggca ttggctatgt
    54541 tgcttcttat ccagaagcaa ggggacaagg cgggatcaat cagatcatgg aaaagatcct
    54601 tgaagattgc cgtgatcgta aagtaagctt atcttatctg gcgccattct cctatccctt
    54661 ctatcggcgc tatggctatg aacaatcatt tgataaaatc agttatcaac taaacagtcg
    54721 tgatgtgccg tttatcaaaa aaaaatctgg aaaaataaag cgtatggatt ttgaagatgc
    54781 aaaagctgca atcagaagaa tttatgaaca gatgccggaa aaccaacgag ctggtctgaa
    54841 gcgcgaagac tggtggtata cgtataaatt caagcaaaaa ggcaattacc agtttgcttt
    54901 gcattttgat gataatgagg aagcaacggg atatatcgtt tatcagcttg caacgtcttc
    54961 ctttattatt gcggagtggg gatttttgga tcatgatgct ttttgcagcc ttgttcgctt
    55021 cgtaagttct cacaatggtg cttttgaaac atttgaatat tcatgcggct acggcggtaa
    55081 tgatcaaaat tatctattag aaaatccatt tgcttctgta atgatcacac cttatatgat
    55141 ggcgaggatc gtagatattt ctttgttttt ggaaaattat ccatttaaaa agagacaagc
    55201 tgcatttgcc ctacaaatag aagaagatca ttatgcgaaa tggaatgaag gaatcgtgga
    55261 agtaacaatc gaaaatggaa aaaatcatct ctcaaaagta gaacaaacaa aactcccaat
    55321 cgttcgagga agtatccaac atatcacgca gctattatta gggtatcgta aagtggaaga
    55381 gttggtattc caagaaagaa tacaggtgac aaaagaatat gaagaaacgt taagctcaat
    55441 cttgccacag caacagccaa ttcttaatga ctatttttga taaaaaacgg gtgatgacaa
    55501 tatttttgtc accacccttt tgtttttcat ttggcatttg aaggaaaatg ttaaagtaaa
    55561 tcctcttttt tcgtaaattt gaattttttg ttttcttttg ttttagggtt ttcttttttg
    55621 tggtcttgga ttgcttcttt cgtaatcttt tcccgatttt ttttagatga atctaagcgt
    55681 tctgcgtata ctttttcttt tggcttatca ttcattggaa aggacctccc tacattattt
    55741 cattctacct ctagtttatc atctgttaag gactagtgga aacgttttgc ctaacttata
    55801 agctgatttt tttagcagat aagcttgcaa gtaaaaaaca aacaagctac aattgattta
    55861 ttgaaattgt tcctgtagct cagtaggata gagcaatcgc ctcctaagcg ataggtcgct
    55921 ggttcaattc cagtcaggaa cgtataaata acccctgtat cctttgcggt acaggggttt
    55981 gttttgtatt tgtgccaaaa atgtgccaaa ctagttatca tttttatata aactatcgaa
    56041 cacagtattg attttttctt tatcctgatc ttcaagctct cgaacaatat gaagataagt
    56101 agacattgta gtttctaaac tgctatgtcc taaacgtttc gatacgctaa gtatatttac
    56161 tccttgataa agtaaaatag atgcatgtgt atgccttagt ccatgcaaag taaattgctt
    56221 ttcaatccct aattctttta attttttgcg caaatatttg ctgacggcgt ttgatgaaac
    56281 taatccattt tttaagttga aaaaaacaaa attatgaggg tttttgattt caaaattctc
    56341 gaataattct ttttgattga ttttaaactt ttttaagaga tcaatcgtat gtttatcaat
    56401 ggaaattttc ctatttgaag tttcgttttt tgtttttccc cattcattta atttataatt
    56461 ccatgtttta ttaatagtga tgatttgatc ttcgaaatcg atatcttccc atgttaatcc
    56521 tagaagttca gcaaatcgca ttccagtggc accagcaact aacacgagca taggagaaga
    56581 gtactgcgca gaaaggtttt cttctacgag tttcattaaa gatttaaatt gatcataatc
    56641 taaatattta tcttcttttg gcttcaatga atcttttcct ttgattactg cttttctagt
    56701 tggatcaaac ggaattaatc cttcttctac ggcatctttc aacgacgctc taatatggtt
    56761 attaaactta ataacggtag attttacatg gtctttagca tacttattta aaaagcgttg
    56821 atatcccact ctatctaaat cagaaatcaa taccgctggc atatatttct ttatgttcat
    56881 taacgtatct tcgtattttc tataagtgat aggagatact gttccttctt tataaagttg
    56941 catccaatcc tcaaagtaat ctgataataa tagatttttg cgatcccctt taaggccttt
    57001 gttcaagtta tattctaatt cgttggcggc atcttttgct tctccttttg tcttaaatcc
    57061 tgattttctt atcttcgtgt attttccgtc atcttttttg tatgaaattt cgtattgcca
    57121 actatttcca cgtttgacta atcttgccat aattgaatca gctctctttc tctgatacaa
    57181 taggaactat aaagaagccc attgtttggg ttttattatt ttttgcacat cctttttgct
    57241 tgccggcgca aggatgtgct atttgtcgtt ttaatggtat aattggatgt gtattagatc
    57301 atccaatcag aggtgtttat tatgattaaa gtgattttta aaagaataac tgaacttcca
    57361 gagtttagat tatttgttga ttattggtat gtctggtttt ttattgttgc attgctgata
    57421 ggtctagatg tcatattaga aagaaaactt aagtaagagt tgagttaata aattgaattc
    57481 ctaaatgaga tattttgtag cccatatttt gtttgccgac atcacctaat actatggagc
    57541 taatcgtttt ttctaacaga ccaaaatcag ataatctatt taagctacca tctattagct
    57601 cttgtgaata gccagacatg ctgatatttc cagtgaaatt ttcgtttcca cgactcatta
    57661 agcatgctaa caattgaatc tcaatcgatg ttaattgatt cagtacaaga agataatact
    57721 cctcgttatc ccatgaagcg ttgttaattc gaagtaaaca attcttataa agatttttat
    57781 acatatctat tttattttgt gcctttgatt tttctacctc gtcatttatc ccttcgaata
    57841 ttccaatgag ttggtccttg ttctctaagt tgaaaacatc ttctggaatt tgatcgcgaa
    57901 tgttttctaa tgattcgttt aaatttttgt agaatttctc gatgcgtttg aaacgcttct
    57961 cgtttttaga cccaaaataa agtgactgca caacgccacc aatataagga attgctccaa
    58021 taccgccttc aactattaat tcaagatatt cttttttggt tagtttttca ttgctattat
    58081 ccatagttac ctcctatttt gtcatttcct ttacaagcat tgtgactcct gatggcactc
    58141 ttttgtatag gttttattat tgtgcacaat ctaactctta gtggggcgtg gttcatttat
    58201 tttttactta ctttactatt aatcatatcc cattgagcat aggatttttc tatgttcgat
    58261 cctcttgaac taatttctcc tgtaaaagtt actttatcgc cctttgtaaa ggtatcagca
    58321 ttgtttaagt ccttcgcaaa aattacatat gaaactttag gtgttgcaga aatatcattc
    58381 caacttttat catcgtactc tttgtctgct ataatggcta ttctagtagt cattgattcg
    58441 ataacagttc cttcaaaagt atatgtttta ccactaatat atttagtaaa a
//
LOCUS       DASNNU010000006        51214 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.006, whole genome shotgun sequence.
ACCESSION   DASNNU010000006 DASNNU010000000
VERSION     DASNNU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.006"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            290..1135
                     /locus_tag="U8116_000347"
     CDS             290..1135
                     /locus_tag="U8116_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN8433367.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="U8116_000348"
     CDS             1416..2636
                     /locus_tag="U8116_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEN8433368.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="U8116_000349"
     CDS             2633..5353
                     /locus_tag="U8116_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN8433369.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="U8116_000350"
     CDS             5337..6281
                     /locus_tag="U8116_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN8433370.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="U8116_000351"
     CDS             complement(6350..7360)
                     /locus_tag="U8116_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN8433371.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="U8116_000352"
     CDS             complement(7540..7884)
                     /locus_tag="U8116_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN8433372.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="U8116_000353"
     CDS             complement(7877..8311)
                     /locus_tag="U8116_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEN8433373.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="U8116_000354"
     CDS             8449..9186
                     /locus_tag="U8116_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8433374.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="U8116_000355"
     CDS             9179..10390
                     /locus_tag="U8116_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433375.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10594..11376
                     /locus_tag="U8116_000356"
     CDS             10594..11376
                     /locus_tag="U8116_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEN8433376.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="U8116_000357"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="U8116_000357"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEN8433377.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="U8116_000358"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="U8116_000358"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEN8433378.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="tadA"
                     /locus_tag="U8116_000359"
     CDS             14092..15021
                     /gene="tadA"
                     /locus_tag="U8116_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEN8433379.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="U8116_000360"
     CDS             14978..16027
                     /locus_tag="U8116_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEN8433380.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="U8116_000361"
     CDS             16024..16302
                     /locus_tag="U8116_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEN8433381.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="U8116_000362"
     CDS             16277..16747
                     /locus_tag="U8116_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEN8433382.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="U8116_000363"
     CDS             16764..17066
                     /locus_tag="U8116_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433383.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="U8116_000364"
     CDS             17083..17454
                     /locus_tag="U8116_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEN8433384.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="U8116_000365"
     CDS             17426..17785
                     /locus_tag="U8116_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433385.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="U8116_000366"
     CDS             17957..18964
                     /locus_tag="U8116_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN8433386.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="U8116_000367"
     CDS             18989..20173
                     /locus_tag="U8116_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEN8433387.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="U8116_000368"
     CDS             complement(20610..22193)
                     /locus_tag="U8116_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN8433388.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="U8116_000369"
     CDS             complement(22771..22914)
                     /locus_tag="U8116_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEN8433389.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="U8116_000370"
     CDS             complement(23137..23616)
                     /locus_tag="U8116_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN8433390.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="U8116_000371"
     CDS             complement(23689..23973)
                     /locus_tag="U8116_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433391.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="U8116_000372"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U8116_000372"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="U8116_000373"
     CDS             complement(24317..25603)
                     /locus_tag="U8116_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEN8433392.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="U8116_000374"
     CDS             25741..26412
                     /locus_tag="U8116_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEN8433393.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="U8116_000375"
     CDS             26378..26866
                     /locus_tag="U8116_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEN8433394.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="U8116_000376"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="U8116_000376"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEN8433395.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="U8116_000377"
     CDS             27827..28591
                     /locus_tag="U8116_000377"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEN8433396.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="U8116_000378"
     CDS             28824..31037
                     /locus_tag="U8116_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEN8433397.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /locus_tag="U8116_000379"
     CDS             31048..31494
                     /locus_tag="U8116_000379"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEN8433398.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="U8116_000380"
     CDS             31665..32924
                     /locus_tag="U8116_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEN8433399.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="U8116_000381"
     CDS             complement(33013..33960)
                     /locus_tag="U8116_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN8433400.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /locus_tag="U8116_000382"
     CDS             34354..35646
                     /locus_tag="U8116_000382"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEN8433401.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="U8116_000383"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="U8116_000383"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEN8433402.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="U8116_000384"
     CDS             37539..38414
                     /locus_tag="U8116_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN8433403.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="U8116_000385"
     CDS             38680..40563
                     /locus_tag="U8116_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN8433404.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="U8116_000386"
     CDS             complement(40638..40922)
                     /locus_tag="U8116_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN8433405.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="U8116_000387"
     CDS             complement(40912..41646)
                     /locus_tag="U8116_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEN8433406.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="U8116_000388"
     CDS             41775..42398
                     /locus_tag="U8116_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEN8433407.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="U8116_000389"
     CDS             42590..43711
                     /locus_tag="U8116_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEN8433408.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="U8116_000390"
     CDS             43718..46846
                     /locus_tag="U8116_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEN8433409.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="U8116_000391"
     CDS             46865..50059
                     /locus_tag="U8116_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEN8433410.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="U8116_000392"
     CDS             complement(50205..50912)
                     /locus_tag="U8116_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN8433411.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16760 a         8948 c        10632 g        14874 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaat taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatt ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gtgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ccagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    51121 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51181 ttaatacagt ctcaatataa ctattcaaac ataa
//
LOCUS       DASNNU010000007        49826 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.007, whole genome shotgun sequence.
ACCESSION   DASNNU010000007 DASNNU010000000
VERSION     DASNNU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.007"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(22..219)
                     /locus_tag="U8116_000393"
     CDS             complement(22..219)
                     /locus_tag="U8116_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEN8433412.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(613..2340)
                     /locus_tag="U8116_000394"
     CDS             complement(613..2340)
                     /locus_tag="U8116_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEN8433413.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2333..3526)
                     /locus_tag="U8116_000395"
     CDS             complement(2333..3526)
                     /locus_tag="U8116_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN8433414.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3713..4357)
                     /locus_tag="U8116_000396"
     CDS             complement(3713..4357)
                     /locus_tag="U8116_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN8433415.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4451..4585)
                     /locus_tag="U8116_000397"
     CDS             complement(4451..4585)
                     /locus_tag="U8116_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEN8433416.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4587..6719)
                     /gene="feoB"
                     /locus_tag="U8116_000398"
     CDS             complement(4587..6719)
                     /gene="feoB"
                     /locus_tag="U8116_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEN8433417.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6716..7189)
                     /locus_tag="U8116_000399"
     CDS             complement(6716..7189)
                     /locus_tag="U8116_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEN8433418.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7590..7814
                     /gene="nrdH"
                     /locus_tag="U8116_000400"
     CDS             7590..7814
                     /gene="nrdH"
                     /locus_tag="U8116_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEN8433419.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7973..10132
                     /gene="nrdE"
                     /locus_tag="U8116_000401"
     CDS             7973..10132
                     /gene="nrdE"
                     /locus_tag="U8116_000401"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEN8433420.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10182..11147
                     /gene="nrdF"
                     /locus_tag="U8116_000402"
     CDS             10182..11147
                     /gene="nrdF"
                     /locus_tag="U8116_000402"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEN8433421.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11236..12375)
                     /locus_tag="U8116_000403"
     CDS             complement(11236..12375)
                     /locus_tag="U8116_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN8433422.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12684..13397)
                     /locus_tag="U8116_000404"
     CDS             complement(12684..13397)
                     /locus_tag="U8116_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEN8433423.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13651..14352)
                     /gene="nagB"
                     /locus_tag="U8116_000405"
     CDS             complement(13651..14352)
                     /gene="nagB"
                     /locus_tag="U8116_000405"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEN8433424.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14586..16118
                     /locus_tag="U8116_000406"
     CDS             14586..16118
                     /locus_tag="U8116_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN8433425.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16309..17511)
                     /locus_tag="U8116_000407"
     CDS             complement(16309..17511)
                     /locus_tag="U8116_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN8433426.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17593..18282)
                     /locus_tag="U8116_000408"
     CDS             complement(17593..18282)
                     /locus_tag="U8116_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN8433427.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18388..18756)
                     /gene="rplL"
                     /locus_tag="U8116_000409"
     CDS             complement(18388..18756)
                     /gene="rplL"
                     /locus_tag="U8116_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEN8433428.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18809..19312)
                     /locus_tag="U8116_000410"
     CDS             complement(18809..19312)
                     /locus_tag="U8116_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEN8433429.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19516..19643)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19722..20411)
                     /gene="rplA"
                     /locus_tag="U8116_000411"
     CDS             complement(19722..20411)
                     /gene="rplA"
                     /locus_tag="U8116_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEN8433430.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20515..20937)
                     /gene="rplK"
                     /locus_tag="U8116_000412"
     CDS             complement(20515..20937)
                     /gene="rplK"
                     /locus_tag="U8116_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEN8433431.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21280..21948
                     /gene="sdaAB"
                     /locus_tag="U8116_000413"
     CDS             21280..21948
                     /gene="sdaAB"
                     /locus_tag="U8116_000413"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEN8433432.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21961..22833
                     /gene="sdaAA"
                     /locus_tag="U8116_000414"
     CDS             21961..22833
                     /gene="sdaAA"
                     /locus_tag="U8116_000414"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEN8433433.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22924..23943)
                     /locus_tag="U8116_000415"
     CDS             complement(22924..23943)
                     /locus_tag="U8116_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN8433434.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKY
                     TKLMD"
     gene            complement(24116..25021)
                     /locus_tag="U8116_000416"
     CDS             complement(24116..25021)
                     /locus_tag="U8116_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN8433435.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25080..25625)
                     /gene="nusG"
                     /locus_tag="U8116_000417"
     CDS             complement(25080..25625)
                     /gene="nusG"
                     /locus_tag="U8116_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEN8433436.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25726..25896)
                     /gene="secE"
                     /locus_tag="U8116_000418"
     CDS             complement(25726..25896)
                     /gene="secE"
                     /locus_tag="U8116_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEN8433437.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25920..26072)
                     /gene="rpmG"
                     /locus_tag="U8116_000419"
     CDS             complement(25920..26072)
                     /gene="rpmG"
                     /locus_tag="U8116_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN8433438.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26234..26878
                     /locus_tag="U8116_000420"
     CDS             26234..26878
                     /locus_tag="U8116_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN8433439.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26934..27437)
                     /locus_tag="U8116_000421"
     CDS             complement(26934..27437)
                     /locus_tag="U8116_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN8433440.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27450..27496)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28034..28999
                     /locus_tag="U8116_000422"
     CDS             28034..28999
                     /locus_tag="U8116_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN8433441.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29098..30270)
                     /locus_tag="U8116_000423"
     CDS             complement(29098..30270)
                     /locus_tag="U8116_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433442.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30257..31402)
                     /locus_tag="U8116_000424"
     CDS             complement(30257..31402)
                     /locus_tag="U8116_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433443.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31416..32340)
                     /locus_tag="U8116_000425"
                     /pseudo
     CDS             complement(31416..32340)
                     /locus_tag="U8116_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32457..33536
                     /locus_tag="U8116_000426"
     CDS             32457..33536
                     /locus_tag="U8116_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN8433444.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33568..34161
                     /locus_tag="U8116_000427"
     CDS             33568..34161
                     /locus_tag="U8116_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN8433445.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(34271..34498)
                     /locus_tag="U8116_000428"
     CDS             complement(34271..34498)
                     /locus_tag="U8116_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN8433446.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34601..37189)
                     /locus_tag="U8116_000429"
     CDS             complement(34601..37189)
                     /locus_tag="U8116_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEN8433447.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37209..37868)
                     /locus_tag="U8116_000430"
     CDS             complement(37209..37868)
                     /locus_tag="U8116_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN8433448.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37917..38420)
                     /gene="trmL"
                     /locus_tag="U8116_000431"
     CDS             complement(37917..38420)
                     /gene="trmL"
                     /locus_tag="U8116_000431"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEN8433449.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38641..39507)
                     /locus_tag="U8116_000432"
     CDS             complement(38641..39507)
                     /locus_tag="U8116_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN8433450.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39616..40257
                     /locus_tag="U8116_000433"
     CDS             39616..40257
                     /locus_tag="U8116_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEN8433451.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40319..41680)
                     /gene="mgtE"
                     /locus_tag="U8116_000434"
     CDS             complement(40319..41680)
                     /gene="mgtE"
                     /locus_tag="U8116_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEN8433452.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41698..42597)
                     /locus_tag="U8116_000435"
     CDS             complement(41698..42597)
                     /locus_tag="U8116_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN8433453.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42599..43396)
                     /locus_tag="U8116_000436"
     CDS             complement(42599..43396)
                     /locus_tag="U8116_000436"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEN8433454.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43374..44078)
                     /locus_tag="U8116_000437"
     CDS             complement(43374..44078)
                     /locus_tag="U8116_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEN8433455.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44209..44785
                     /locus_tag="U8116_000438"
                     /pseudo
     CDS             44209..44785
                     /locus_tag="U8116_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            44903..45580
                     /locus_tag="U8116_000439"
     CDS             44903..45580
                     /locus_tag="U8116_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN8433456.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45659..47467)
                     /gene="pepF"
                     /locus_tag="U8116_000440"
     CDS             complement(45659..47467)
                     /gene="pepF"
                     /locus_tag="U8116_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEN8433457.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47643..48641)
                     /locus_tag="U8116_000441"
     CDS             complement(47643..48641)
                     /locus_tag="U8116_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEN8433458.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48867..49520)
                     /locus_tag="U8116_000442"
     CDS             complement(48867..49520)
                     /locus_tag="U8116_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEN8433459.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14907 a        10467 c         8758 g        15694 t
ORIGIN      
        1 tgcttagcat tttttttcat tttagcactt ccctggaatt gctccaccta aaaaaccacc
       61 tatagacatt cctgcaatac aagtagttgc cttggcccaa tcgaccgtac atttattttt
      121 agtgcaatac actccatttc cataatattt tccactatga gtggtacccc catagataag
      181 ttgtgtctct ttaatagaca aaatttttaa atgtttcata atctaatcac ccctaaatta
      241 atgtttttag actattattc taacattcat taaatttcaa aaaacaattc cttcttgaaa
      301 cgtagcaaaa taattcctga ataagaattt aatcactttt aagtatataa actatgtata
      361 ttctttaata ttttttgaga atgcacttcg tacataataa actttaatca cattagtctt
      421 tcatacctac gatcgatctc tttgactcgc acccataaat tcataatagt ttgtcggcgt
      481 ctttggacgt ttcgaagatg agctacgcat tctctttttt tatagacaaa aaagatagag
      541 acgaacatgg agcttccaaa tttggatatt tgatccaatt ttggaagctc catttatttc
      601 gtgtctgtct ttttattttt tccagctttc gactaattct tggttctctt tgatccagtc
      661 ttttgctgct tgctgtggat cttttccatt gttgatttcc aacataactt tttccatatc
      721 tttttctgac caatggaaat tgtctaatac tttgtaagct tctggctggt cttctttcaa
      781 tccttttcga accatcgtat ggatctgctc ttcgcttccc attccgtttt caggatcttc
      841 taagtatttc agatcatatt tggcaaacat ccaatgaggt gtccatcctg taactacaat
      901 tggttcgtgt tttttgatcg cttgacctag tgcaacggtc attgctcctg aagaagatgt
      961 ctctactttc cagtctttca agttgtcata tttttggatc gtgttttcag cagccgtaac
     1021 tacaccagct cctggctcga ttcctatgat tttcttacct gcctgatcgg ttaactcatc
     1081 gatcgaattt acatccatgt aggctggcac tgctaaacca acttttgctc ctgtcaaatt
     1141 ggcacctagg tcttcgactt gatctttata ttgtgcatat tgtgaaccat gagttttagg
     1201 tagccaagct gaaaccatgg catctgactc gccatttgaa acagatttcc acatgattga
     1261 attatctaat ggcgtcatcg ttacatcata gcccatttgt ttcaatactt caccgactac
     1321 atttgttgaa gcaacttctg tatcccattc aacataagat aagttgatac gttttgtttc
     1381 tttggcagat gaaaaactcc ctataccaat cgcacctaag atcactacta ctacagcacc
     1441 aatgatcagt ccttgacgtt ttttactttt ctgtgttgtt gctcctgcag cttttttatt
     1501 gtttggctgg ttcaaatgct gtgtaaaacg gtcaatgatg attgctaaga taactaatgc
     1561 aaccccgttg acaaatccat tcccgacttg tgctctttgc aatgctgata atacgccgcg
     1621 tcctaagccg ggtgcaccga tcatagaggc aataacgacc attgaaagtg acagcattgt
     1681 tgtttgattg atccctgcca tgatcgtact tttagctaaa ggcaattcta gtttgaatag
     1741 tttttgacga cttgtgctcc cgaatgaatc agaagcttct actaattctt taggaacttg
     1801 tcgaatccct aaattcgtaa aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa
     1861 tacccctgga accatcccga taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc
     1921 tggcattgtc tgcataaagt caaggatcgg tttgataata ctttgggctt tactgctttt
     1981 tgccatcaag atccctaatg gtaccccaat gatgatcgag atcacactag ataaaagaac
     2041 aagtgtgaca gtattcatca aatcattcca tagaccttgg ttataaataa accatagacc
     2101 gattaatgtg aacaacgtaa gtcctggacg tttcttagaa ataaagaaag ctgctaccgt
     2161 caataacaag ataaacaaag gagctggaat cactaacagt aaatttgtaa tccccgacat
     2221 cactgcctgt ccaatcgttt gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca
     2281 ttctgtgata ttttctaccc agtttgccac tggtaattga tagttcaata gattattcat
     2341 gttcactcac ctcgcttgtt tctgctaatg cttcaatcac actacctcgg atgacgacac
     2401 cttttaattt tccatcctct acaactgcaa gtggagtcgg tgagtcataa ataatattga
     2461 aaatatcact taccaccatg tctttcccaa tcaccgtcac attttcatct aggaagtcga
     2521 tcaatggacg tttttgcttc ctagcttcta acgcttgatc tgcagtgatg atccctttca
     2581 ggtgtctctt tctgtcaaca gccatcagca tgctgacttc ttcattacgc atccttgtca
     2641 atgcaacgtt tggtccatca gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt
     2701 gagccgtaag cactttggaa cgatcgactt cttccacaaa ttcacgtaca taatcatttg
     2761 ctggattggt caatatttct tcacctgttc cgatctgcat gatttcgccg tctttcatca
     2821 aggcgattct gtccccaatc cgtaaagctt cattcaaatc atgggtaatg aaaataatcg
     2881 ttttttgaac attggcttgc aggtccagta attcgtcttg catttctcgc cggatcagcg
     2941 gatcaagagc cgagaacgct tcgtccatca gcaagatctc aggatcattt gctaaagctc
     3001 tggctaggcc aacccgttgt tgcatcccac cagataactg actaggatat tggtctttaa
     3061 atgaaagcaa actagaattt tctaatgctt tttctgcttt ttcttgtcgt tcttctttag
     3121 gtacaccacg gacttctaac ccgtattctg tattttccaa gatcgttcga tgtggaaaca
     3181 gaccgaagtt ttggaaaacc atattgattt tatgacgacg gacttctcgc aattcttctt
     3241 tgcttaactt cgatacatct tctccatcaa tatagatact gcccgaagtt ggctcaatca
     3301 gacgattcaa tagacggatc aatgtggatt tcccactccc ggaaagcccc atgatcacga
     3361 agatttctcc ttcttttacg tcaaaattca cgtcatacac accaaccgtt gcaccggttt
     3421 tctttaaaat ctccgttttc ggctggtgat cattcatcat atctaaagcg gcttgtgtct
     3481 ttttaccaaa tatctttgtt aaatgtttta cttctacttt taccaaacaa attctccttt
     3541 tttatgtaaa ttcatttttc tttctgaaaa aagtgaccac actatgttaa cgtcatgtag
     3601 tcactttgtc aaaaatgaaa gaaatttgct tgtccaaact gaaaaaaact tggctaaatc
     3661 tttgtaaata aaaggtttag ccaagttaat atttttttaa tatttcacac aatcattctg
     3721 caagctgatg ctccgggtaa aaaaagtttg tcatacgctg tagtgtatat tcatttccaa
     3781 taaaatgaac gatgtcccct gccgtaaatt tagcataggg gccaggtgaa acaagcagtt
     3841 cttcattatg agaaattgcg ataactgtgg cgccagtcgc ctgccagata ttcaactcgc
     3901 taattgaacg gtccagatgc attgcttttt ctgataaaga taattcaaac ggaagtaaag
     3961 gattcatctt atgtattcgc ttcgtttgat tgaccagctg atccaataat tgagaaaaat
     4021 gattcagctc atcttgttgc ttctccacac ttgccatgat ttctgctttt gtatcttgga
     4081 tggattgtac atcttgatat tgcaatagga aatcttttgc tttttccttc gaagcaacaa
     4141 atgccccact tccatgccgt acttccatga ttccaagatc taccagcaca ttgatagcct
     4201 tgcgggtagt ctcaggagaa gcattgtacg tactggcaag tgttgagcgg gcatggatct
     4261 tctcacccac tttatatcgg ttttcaacga tacgttcggc taaatcaaca gcaatctgct
     4321 ggtagcgcgg gcggacgatt tttttctttc cagacatttt ctttcctctt tcttttttta
     4381 ctttccctca tcttactatg ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg
     4441 caacgactga tcatcgcttg atctgttcct tttttactgg gcaagctgtt tgacagtcat
     4501 cacaattctt accggatttt aaacgagtat agacaattgc gcctgcactt ccaaagatta
     4561 acacacttaa taagaatgtc gccatcctat tcctctcctt ctaatgacaa tatagtataa
     4621 aactggttct tggattttgg ctgacggaac aaaacataaa cgagtcctat catcagtaaa
     4681 atagcaagat acgtccctaa cgctacctgt ccaccctcca ataagtgtcc caactgataa
     4741 atcacaaaac taactaaata agctaaaccg cattgatagc ctatggcgat ccaagtccat
     4801 ttcttggcat tcatttcccg atggatagcc ccaattgccg caaaacaagg cgcacaaagt
     4861 aaattgaagg ctaacaatga ataagctgct gcgggagtat atgtgtgttg tagcacttgc
     4921 cagatttctc tgccattttc ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca
     4981 ataatatttt ctttagcgac caatcctgta atcgctgcga ctgttccttg ccagtttccc
     5041 caaccaagcg gctgaaaaac aactgccagg attcgcccga aaaaggctaa gatactttgg
     5101 tcttcagaaa caggttgagc ataaaaatta taagaagagc tgaaccagat cacgatacta
     5161 gaaacaaaga taatcgttcc tgctttttta acaaaagact tgctgcggtc ataggtttgt
     5221 cttaggaccg ctgaccattg aggaaaatga tatgcgggca attccatgat aaaaggagct
     5281 gtatctccag caaacaaacg tgtttttttc aagctgattc cagagaaaat aattgcagtc
     5341 ataccaagaa aataggcaga aggagctacc caagattgat gaggaaaaaa ggcccctgct
     5401 actaaggcaa taatcggcaa ctttgcagaa caaggcatga aggttgtgac catgatcgtc
     5461 aaacgacgat cttgctcgtt ttcaatcgtg cgactggcca tgacgcctgg cactccacat
     5521 cctgtagcaa tcagcatagg aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt
     5581 cgatccatga caaaagcgat cctcgccata tatccgcagt cttccagaaa gctcaagcat
     5641 aaaaacaata taatcaattg cggtagaaat cctaacactg ctcccacgcc tgcgatgatg
     5701 ccgttcaaga tcagctcctg catccatccc gctacttgcc attcagctaa caatctgcca
     5761 acatgttctg gtaaccatgt accaaacaat gtgtcattga tccaatctgt ccccattgtt
     5821 cctactgttt gaatagccag ataatagatc agccacatca caaatgcgaa gatcggcaag
     5881 gctaaccaac gattcgttgc aatctgatcg attttgtcac tcatcgataa ttggaaagaa
     5941 tcatttgaca caacacagag tgtacatagt tgggtaataa attcataacg ttcattgacc
     6001 agaatcgtct cactatcgtc gtccaatagt ttctctgtta actggatcag ttgctcgatc
     6061 tctttttgct gagaaactgc tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa
     6121 tatttcattg cataccagcg tttcttgccg ctttgttctc ctagtacatc ttcaatttca
     6181 tgtaaagctg cttctaaacg tggatcatat gtacagggaa caggaataga aggccgcttt
     6241 tttgcagact cctgaatctt ttcgattagt ttttcgattc cttgcttttt caatacgctg
     6301 attggtaaaa ctggtacacc aagaccatag gaaagctttt ctagattgat tttttttcct
     6361 tcttttttga tgacatccat catattcaga ccaatgatca taggaatgtc taaatccaat
     6421 aactgcgtag ttaaataaag atttcgttct aaattggtag catcaataat gttgatgatc
     6481 agttcaggct gttcctgaac caaataatct cttgcaatca cttcttctgg tgtataagga
     6541 gacaaagaat agatgccggg taggtcctga atatgaatgt tcggattttt tttcaacgat
     6601 ccttcttttt tttctactgt tacgcccggc caattcccta catattgatt cgaaccagtc
     6661 aagagattga acaagctagt tttcccgctg tttggatttc cgactaacgc gatattcatt
     6721 ctttttcctc cctttgtatc aagattagtt cggcctctga ctttcgtaac gtcagttcat
     6781 accctcgcac tgtcagttcg attggatctc ccagaggggc tagttttcgg ataactattt
     6841 ttacatttcg cgtcaatccc atatccatta accgtcgctt cactgcgcct tgtccgtgga
     6901 ttgctacgac gatcccagtc tctcctgctt ttaattgatc aagagaagtc cagctttcag
     6961 aggaagcagt ctttttattg acaaagattt gacctagtat ttcccgactt actgccactc
     7021 gatgactttt taccagtaaa atggcattgt tgcctgataa attgacgacg acgattttac
     7081 ttccggcaac gatcccgata ttttgcaaat ggcgttttgt tttttcttct gtttcgattc
     7141 gttcgacttg ataaacgaaa ttctgctttg cctgcgctaa ggtctgcata ttgattcctc
     7201 tcttgtgaaa taaatcatgt attaaaatca ttcattatat gttctaattt tcttacgtgt
     7261 tacgcaaata aatgagaatc attctcacat agaatactct cggtaattga gaatgtcaat
     7321 tatacttgtg atattatgag cgaatcttta gtattgatag ataaaaatga gaaaaaaata
     7381 tttcttcata gttttccaca attttttctc tattaaattt gctattttcc cacacgatac
     7441 ttgttttcag ctcttttttc aaaaacacaa aacttagtat tccacagttt ccacaacaca
     7501 atatgtagtg tttgttgttt gactttttta ctaagagttt tatactatct attatagata
     7561 ataagaatgt gttagaggag gcactcacca tgaacgtaaa aatcttttct aaaaataatt
     7621 gtatgcaatg taagatggtc aaacggtttt taacagaaaa tcaaattgct tttgaagaag
     7681 tcaatatcga tgaacagcct gaagcagtag actggttgaa agcacaagga ttccaaagtg
     7741 ttcctgtcat tacttctgat gcagaaacag ttatcggatt ccgtcctgat cagttgcgta
     7801 aacttgccag ctaacatgac taaaagaatc aaaacgtgat acatggattt gaagggcttg
     7861 agacagcacc agtttttgtc gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc
     7921 aatgatttgc aatcaagcat catcaaatac gaaatgaagg ttgggcgtac gtatgagtct
     7981 taaaaattta caagatgtca gttatttcaa gctgaataat gaaatcaatc ggccagtcaa
     8041 tggtcaaatc cctttgaata aggataaaga agctttagtt gccttttttg aggaaaatgt
     8101 caaaccaaat acgatgactt ttctatctat tatggaaaaa atcaattact tgatcgatca
     8161 agaatatata gaagcagaat tcattcaatt gtattcacca tcatttattg aaaagctata
     8221 tcaatttttg gacgagcaac atttttcttt caaatctttt atggctgctt acaaatttta
     8281 ttctcaatat gcgttgaaaa ccaacgacgg ttccgcttat ctggaaactt atgaagatcg
     8341 agtagccttc aatgcgcttt actttgcgaa cggggatgaa gaattagcac ttgctcttgc
     8401 agacgaaatg atccatcaac gatatcagcc agctacacct tctttcttga atgctggacg
     8461 taaacgtcgg ggcgaattgg tgtcttgttt cttgactcaa gtcactgacg atatgaactc
     8521 tatcgggcgc tcaatcaact ctgctttgca attgtcccgt attggtggcg gcgtagggat
     8581 tactttatca aatcttcgtg aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag
     8641 tggtgtcgtt ccagtcatga aattattcga agatagtttc agctattcta atcaattagg
     8701 tcagcgtcaa ggagctggcg tggtttattt gaacgttttc catccagaca ttattgcttt
     8761 cctttcagca aaaaaagaaa atgcagatga aaagattcgc gtaaaaactc tttctcttgg
     8821 cgtgatcgta cctgataaat tttatgaatt gacacgtaaa aacgaagata tgtatttatt
     8881 tagtccatat agtgtcgaaa aagaatatgg tgtcccattc tcttatgtaa atattacagc
     8941 tgagtatgat catctagttg caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga
     9001 tcttgaaaat gaaatctcaa aattacaaca agaatctggc tatccttacg taatcaatat
     9061 tgatacagcg aaccgcgaga atccaatcga tggcaagatc atcatgagta atttatgttc
     9121 agagatttta caagttcaaa cgccttctgt gattaacggc aaacaggaat atgaagtatt
     9181 aggaacggat atctcatgta acttaggctc gacaaatatc gtgaatttga tgcaaagccc
     9241 taactttggt caatctgtgc gtacgatgac tcgtgcattg acatttatta cagatgcatc
     9301 tgatatcgat gtcgtaccac cgatccaaaa cggaaataag ctgaatcata cgattggatt
     9361 aggtgccatg ggcttacaca cttatcttgc aaaagaacaa atggaatatg gctcagagga
     9421 ttcattagac tttacaaata tttacttcat gctgttgaat tattggacac ttgttgaaag
     9481 caacaatatc gctcgtgaaa gaaaacaagt tttccataat tttgaaaaat cagcttatgc
     9541 tgacggcact tatttcgaaa aatacgtaac tggagaattc cagccaaaat cagaaaaagt
     9601 caaagcattg tttgaaggca tttttatccc aactgctgaa gattggaatg ctttgaaaca
     9661 agcagtaatg aaagatggac tgtatcatca aaatcgcttg gcagtagcac caaatggctc
     9721 aatttcatat atcaacgata cgagtgcaag tattcatcct atcactcgca tgatcgaaga
     9781 acgccaagag aagaaaatcg gtaaaattta ttacccagcg ccttatctat ccaatgagac
     9841 gattccgtat tacacttctg cttatgatat ggacatgcgt aaagtaatcg atgtttatgc
     9901 aatggcacaa caacacgtcg atcaaggcat gagtttgact ttattcatgc gttctgaaat
     9961 cccagaaggc atttatgaat ggaagacaac aacgaagcaa acgacacgtg atttgaatat
    10021 tttaagacat tacgctttcc acaaaggaat caaatccatt tattacgtac gtacctttac
    10081 tgatgatgca gaagaaatcg gaagcaatca atgtgaaagc tgcgtgatct aatatcatag
    10141 atagattcgc ttattcatat tttaattaaa ggagcattct catgtcagaa acatattatg
    10201 aagcaataaa ctggaatgaa attgaagata tcattgataa atccacttgg gagaagctga
    10261 cagagcaatt ttggctagat acacgtatcc cactttcaaa tgacctagat gattggcgta
    10321 cattgtctca attggaaaaa gatacagtag gacatgtatt tggcggtttg acactgctag
    10381 atactgtcca atctgaaagt ggcatggatc agttgcgtaa agatgtccgc actccccatg
    10441 aagaagccgt tttgaacaat attcaattca tggaatccgt tcatgccaaa agttattctt
    10501 ctatttttag tacactgaat acgaaaaaag aaatcgaaga aatttttgac tggacaaata
    10561 cgaatcctta tctgcaaaaa aaagctgagc gtatcaatga aatctacaaa cacggtacac
    10621 cgctagaaaa gaaaattgcc agcgtctttt tagaaacatt cttgttctat tcaggttttt
    10681 atactcctct ttattattta ggaaacaaca aattggctaa cgtagcagag atcatcaaac
    10741 taatcatccg agatgaatct gtacatggaa cctacatcgg ttacaaattc caattaggat
    10801 tcaacgagtt acctgaagca gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt
    10861 atgagcttta cgaaaacgaa gaacgttata cagaagaatt atacgatcca atcggctgga
    10921 cagaagaagt caagacattc ctacgctata atgccaacaa agcattgatg aacctaggga
    10981 tggatccact ctttccagat actgccaatg atgtcaaccc aatcgtaatg aacgggattt
    11041 caacaggcac aagcaaccat gacttcttct cacaagtcgg aaacggctat ttgttaggtc
    11101 atgtagaagc gatgaaagac gacgattatt tgattggatt ggaataagga tttattttaa
    11161 ttcaaaaatt tttcgaaaaa taaaaagagt ttagcgtaca gatattttaa atgtacactg
    11221 aactcttttt tatgcttatt cacttgtttc ttccagcact tttggactgt ctaacttttc
    11281 cgaaacatgt tccattcttc tcaataaata gattttccgg atatttaata ttatcgtctt
    11341 aatcactgca tagacaggta tgcaaatcag catgcctaat attcctgcga cacttcctgc
    11401 tccaatcaat accaagatga tcgtcaaagg atggatattc attgatttcc caaataacaa
    11461 cggcttgatc aaatttccat ctaattgttg gatcactaaa atcgacaacg ccatatacaa
    11521 agcttgaaat ggagagataa acaaaccgac aatgattgct ggtaccgctc caataaaagg
    11581 gccgacatac gggataatat tcgtgattcc gcaaatgacc gcaagcaaca agctataagg
    11641 ctgatcgaat attctcattg ccaaaaagct cattacacca ataatcaaag catctaagat
    11701 cgtactgctg atataggcag ataatgtttc gtttaattct tttaccgttt ggcgtaactc
    11761 tttacgaatc gctttcggaa agaagtgaga caacgcatca ataaatttgt gcccatcctt
    11821 gaacataaat agcaaaataa atggaacggt aaataacaaa ataaaaaatt gcatcataaa
    11881 tgaaatgatt tttgagacac ttgcagataa actgactaat atgatattga acaaattact
    11941 aagagataaa tttgctgatg ctaattgttc ttccaaattg aaatttttaa actcttcccg
    12001 ctgcgccaat tcatttaacc atttgcttgt ttcatctgca tacattggca agctctgggt
    12061 caattgaatt gtctgttcca ttaactgagg cactacattc atgacagtta aaacaactaa
    12121 aatgattacg accaaaaacg tcaataaaaa accgaacact ctcgggatcc gatgcttttc
    12181 gagaaaaaca acgattggat cgaacatata atagataaaa ccagcaacaa gcaatggtaa
    12241 aaaaactgcc gaaagcattt tcaatattgg actaaaaata ttcggcattt gtaaaataaa
    12301 gtacacgccg atgatcgtga gtactagctc cactgtccag aaaaataatt ttgattgttt
    12361 aaaacgtgat tgcatacatc tcctccaagg tcctttgata cttctattct atcatttaac
    12421 aaagagttac gcgctgagat tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt
    12481 taatgagtag acgagtactt aaacaaaaac acggtttgga gtagtgacaa aaattatact
    12541 gcagtccctt tttccaaata aacggtgttc aaaagcttgc atcttcgtaa taagcccagc
    12601 caaacgaaaa atataaggaa ctatttttat ttggctgggc ttattacttg atgtaggaca
    12661 tctattttac aacctcagat catttattgc gcaatactga ctaaatgcaa ctttgtgata
    12721 tttttgtgta cggacaactc ctcgatcaca tcggcataag tgagcccttt tggaagatca
    12781 atcgtataga tatttttgta cactcggtag tcatcaacga attttacgtc aaaattcaca
    12841 tcttcgatta cgatattttt ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt
    12901 ctatggataa attggatttc taacttttta gtcgtaggta catggatcac gcgtttcact
    12961 actgtgagtg caatagcaat cccgataaaa ctgatgatcg caatggaata gtaacccatg
    13021 ccgatggcga tccctaatcc agcaattgcc cataaagaag cggcagtcgt aagtcccgtc
    13081 accgattgtt tcgtgacgat gatcgttccc gcgcctaaga aaccgacacc actcaccact
    13141 tgagcaatca gccttgcttc atctgaacga atcgttccag caagctgtgg atactctaaa
    13201 gcatctctta atgcaccact agcgatctca acttggatca atgcaatgat acatgctccc
    13261 atacaaacta aaatatgcgt acgcatacct gctggacgat ttttatattg tcgttcaaaa
    13321 ccgattgctc cgccaaatag catagcgatc cctaaacgta aaataatctc tggaattgtc
    13381 aataccatat cattcatcta aacaccctcc agtccaatat atcatgtctg aaaggagcag
    13441 acaaaaaaag tgcaattttt acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat
    13501 agtccttttt atgaataaac ggtgttcaat cagctgaccc tcggtattac tcaacaaacg
    13561 gcaaaatatg gggagctatt tttcctttgt cttctctaat actcaggtca aaacgctgat
    13621 ttcacaacct ctttagccta ctattgaaaa ttacaactta cttgctgctt cttcatcgat
    13681 gatcacaaca acattgtcgt gattttgcaa tacacttgct ggcaaatgat tcgttacagg
    13741 tccttcaatc atccctttga ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat
    13801 ttctttactt ttcataattg aaccgattcc cattgaaaca gcttttgttg gtacatcttc
    13861 tgctttatcg aagaatcgtt tgtttgcatt gattgttgat tctgttaagt caactactga
    13921 agtcgttccg tcaaatgaag caccaggttc attgaaaccg atatgtccat tttgcccgat
    13981 tcctaagatt tggatatcga ttggatgtgc atcgatgatt tgatcatact ttttgcatgc
    14041 tgcttccaga tcttctgctt ttccatctgg tacaaacgtt tctttgaatg gttttttatc
    14101 aaataattgg acatccatga aatgacggta gctttgttca tctgaaccgc ctagtccaac
    14161 gtactcatct aagtttacag aagtcatttc actgaaatcc aaatcgcttg aagtcatttc
    14221 tttatataaa gtaactggtg tgcttcctgt tgccaatcct aatacttttg cacctttgtt
    14281 cataccttct ttgatgattt caaatgcttt ttttccgcct tcttcagcgt tttttacacg
    14341 aatgatttcc atagtgattg ctcctttata ttttggtata gcccaattat agcattcttt
    14401 ttttcttttt acaataatta gtatagacca aattgcattt ttttcatttt cttgacaata
    14461 aataggaatc cgtgttaggt ttcataagat ggttcaatca aacaatccat gttgcatgaa
    14521 aaccaagcaa gtgattcact gtggacgctt tatgtctgca gtttttttat gaaaaaggag
    14581 ttattatgag tatactgaca ttaaacaaca ttacccagca atttggcgac aaaatcctct
    14641 atgaaggtgt cgatctgcaa ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg
    14701 gagccggtaa aagtacattg attaaaatca tcactggtga gatcttacca gatgacggac
    14761 agatccattg gcaaaaaaat attcacaaag gttacctcga tcaatatgtg gaagtcgatg
    14821 aaacattaac aatcgaagaa tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag
    14881 aaaaaattgc ttttctttac caaacatata gcgagacctt cgatgattcc ttgctggaaa
    14941 aagctgggaa actccaaacg gaattagacc aaggagtgtt ttatcaaatc gacaccttag
    15001 ttgctgagat gagcagcggg ttggggatcg atgtcttagg tttagattca ctgttgaaaa
    15061 atctaagtgg tggacaacgt tcaaaggtga ttttagcaaa actactttta gaaaacccag
    15121 atgttctgat cttagatgaa ccaaccaatc acttagatga tcagcatatc caatggctga
    15181 cgcagtttct tcaggatttt gacggtgcgt atatcgtgat ttcccatgac caagaatttc
    15241 ttgatcagat caccacccat atcgcagaca tcgagtttgg aaagattact aagtatactg
    15301 gccatctaaa acaagcattg aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat
    15361 atcatgctca acaaaaacaa atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg
    15421 gttcccgatc aacgatggca aaaagccgac aaaaacaatt agataaaatc gaacggctga
    15481 cgcctccagc ttcagcaagt aaagctgtct ttgattttcc attctcgcct attgtcacga
    15541 cattagcagt ggaaacaact gatttagtca tcggatatga tcgaccgcta cttgcaccca
    15601 tcaatttgac catccgcttt ggtgaaaata ttgccattcg aggcttcaac gggattggaa
    15661 aatctacttt attaaaaaca ctgatcggtg agattgaaaa aataagtggc gatttccact
    15721 tcccagataa cacaaaaatc aattactttt cacaagatct gtactgggaa aatccattag
    15781 aaacaccact acaatattta agcagtcaat ttccaaaagc gactatcaaa gaattacgtc
    15841 gccaactagc taaagcaggg ttagttaatc aattagcaag tgaacctctc actacattaa
    15901 gtggtgggga acaaactaag gtgaaacttg cacagttgac aatgaaccaa ggtaatttac
    15961 tgattctcga tgaaccgacg aaccatatcg atcaagaaac gaaagaaagc ttacaagacg
    16021 ggatacagaa atttccagga acagtaatca tcgtctctca tgaacaagaa ttttatcaag
    16081 atcttgtgca acgagtcatt gagattgaac caaaataaac gtttgattga atagttgaaa
    16141 aaaaggagct gtgacaaaag ttttgtcaca actccttctt ctgaataaat ggtggccaat
    16201 tagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg
    16261 ctattcttat tacttgatgc agaacagctt ttcacaacct cttttcgtct attttttatt
    16321 ttcgagattt ctctttcctt taaaaacaac tgtttgaaag aagaacgcac atccttgaaa
    16381 aacaagcccg ctaaccgtta cacctaacca gccatactgc tgccacattg tcgtccctag
    16441 caatgaacca gccgcacctc caataaagta cataaacata aatacagtat tatttcggtt
    16501 gcttgctgct tcacctagcc cctgtactcg tgtttgattg gcgacttgac caaattgatt
    16561 cccgacgtct aataaaataa tagctaaaag caaaatggcc aaatggcttc cgccaaccaa
    16621 taaagcaata aagcttattg tttgcataac aagtcccata tacacgattt ttctttcaga
    16681 ataagaatct gccagtcgtc ccacgatagg tgcagcaact gctccagaca aaccaaaaat
    16741 cgctaatatc cctgcttcga atgttcccca attgtaggcg ggactgctga tataaaaaat
    16801 caacgtagac caaaagatcg aaaatgttcc aaacatgaaa aatccactga ctgccgcttc
    16861 tcttaataga cgttgagaag caactaaatg cggcaaactt ttaagtgaat caaggtatga
    16921 aacagctgta ctactttgct ggacagaacg cggcattttc cagtaaagca gaagtgtaag
    16981 aattccgact gcacataaag caaataaata gatcatccgc caagaaaaaa gactggcaac
    17041 aataccagat accgtccgag aaagcaaaat ccccacgagt aaaccgctaa gcaatgtacc
    17101 catgaccttc cctcttgctt ttggtcccgc tagtaccgca ccgtaaggaa tgatgatttg
    17161 cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat gcaaataacg taaacgaagg
    17221 cgcaaaaaaa gcggccaaca gagagacggc agataacgct gccattcgaa tgatcaattt
    17281 tggacgatcc accacgtccc ctaaaggaac taaaaacagt aaccctaacg catagcctaa
    17341 ttgggtcaac atcgtcaata tcccgactgc acttgtgcta acagataaac tagctgctac
    17401 ttttgttcca atcggctgga tgtaatacat attggcaaca acaacgccac aagtgattga
    17461 taatagtaaa ataagcgaag ttgttaaagg ttttttttca ttactcttca ttgatttccc
    17521 ccatttccta tcttagttta caacttatgg aaaagattgg aaatagaaaa atacaactat
    17581 tcgccacact tattatttgt gcttttgttt tccttgtttt ttcttgcgat ctttataaat
    17641 gaagacaact aaccctacta gcacagtaac acttgcaacc aacgtgactg cagttttgtt
    17701 atatccgtga aacagagctt ctccaccaca cgcatacaac acagacgaag ggatgactcc
    17761 aatcacgatc gacaatatga tttgctgtag aggcaatttc atcgcatctg cagcaaaatt
    17821 gaccatagaa gatggaataa ccggtatcat atagcctaac attacaccta tttttggatg
    17881 tttcatttgt cggattgctt gcacccaatg attcgtttcg gttttcttat ctaaaaattt
    17941 taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga gcattaccca aaatgttgat
    18001 gatcgcacct gtcagtggtc caaagctcaa accagcaatg actccaacta ctgaagtagg
    18061 aatacctgga acagcacaca tcaccgctgt caatacgatc aagagtatcg atgcaaaagt
    18121 cccatgcgaa cgaatatgtg cgagtagctc tgccttactc gctctcggat ttaaaaataa
    18181 ttggatgtct gtctgatatt ctaaatacaa ccgatagccc actaccagta tcagaatgat
    18241 tcccaacgag atcaatatca atcggattat tttctttttc atatttcctc ctgaaaaaaa
    18301 tctatattca ttaaataata gtttaacata aaaacaaaaa agcaccgaaa tccaacatag
    18361 gatctcagtg cttttaaata gtaaaaatta tttaacagtt acagatgcgc caacttcttc
    18421 aagtttagct ttcaattctt cagcttcttc tttagatacg ccttctttaa ctggtgcagg
    18481 agcgccatca actacagctt tagcttcttt caagcctaaa ccagttgctt cacgaactgc
    18541 tttgataact ttaacttttt ggtctccagc tgcagttaat tctacagtga attctgtttg
    18601 ttcttcagca gcagcagcgc caccagcagc agctacagct acaggagcag cagcagatac
    18661 gccaaattct tcttcaatag ctttaactag gtcgttaagt tctaggatag ttgcttcttt
    18721 taactcagca acgatgtttt caatgttcaa tgccattata atttcctccg tttaattgtt
    18781 cttttttatt taatgcttta tgcggcaatt aagctgcatc tccatcttct ttgtctgcca
    18841 ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg tagtacagat agcaacatag
    18901 atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt gatttcttcc aatgaagaga
    18961 ctttaccttc gatgataccg cctttgattt ctaatgcttt tgcttcttta gcaaattcgt
    19021 ccatgatttt cgctggtgcg actacatctt cgttactgaa tgcaacagct gtaggtccag
    19081 tgaatacttc gtccatgcct tcaaggccag cttttttagc tgcacgtgaa agaatagaat
    19141 ttttgataac tttcatttct acgttagcat cacgaagttg tttacgtaaa cgtgttactt
    19201 catcaactgt taatccgcgg tagtcaacga ctactactga tgcagctgct gtaaattttt
    19261 cgcttacttc atcaacgatt gctgcttttt ttgcgataat tgcttcactc atttttgagt
    19321 ttcacctcct gaattttgat ggaagagttt tttgcactga aaaggcaagt cacttttata
    19381 gttactttgc tcaaccagta ttattcatca cctattcact tcgttcatag tgattctact
    19441 cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc agtattgttc atcatctctt
    19501 cacttcgttc atagtgattc acaataaaaa actctatgcc accgcagaca tagagggact
    19561 attgaatata ttcaataaac gtcctcggta ggaaattaag gcttgatgcc acctactgtc
    19621 ttcggtacaa gttaattatt aacctcaact agcttagcat gctaaactag ttgagtcaag
    19681 aattattttt cagttggatc aatcaacatt ctaatattta attagaaaga agcttggtca
    19741 acatggatac caggtccaaa tgttgttgtg atcgttacat ttttcatgta ttgaccttta
    19801 gctgctgatg gtttagcttt tagcaataca tcatggattg ctgcaaagtt ttcaactaat
    19861 ttttcatcgt caaatgaaac ttttccgatt ggtacatgga tatttccagc tttgtccaca
    19921 cggtaagtta ctttaccagc tttcacttcg tttactgctt ttgttacgtc cattgtaact
    19981 gtaccagttt tagggtttgg cattaagcct tttggtccta atactcgtcc tagacgacct
    20041 acagtagcca tcatatcagg tgttgcaaca actacatcaa agtcaaacca tccgttttgg
    20101 attttttgaa ccatgtcgtc gtcacctacg aaatctgcac cagctgcttc agcttctttt
    20161 gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt taccagttcc gtttggtaat
    20221 acaacagcac cacggatttg ttggtctgct tttttagggt ctacgttcaa acggtatgca
    20281 acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt ttgctaaagc tactgcttcg
    20341 gcaacaggat aaactttact tgaatcaact ttttttaatg catcttgcat ttttttgctc
    20401 tttttagcca ttttggttcc tccttgattg tggttataac ggttgtacct cccacgtgtt
    20461 ctatatcttt caatatagag ccaactgaga agctgctgtc ttagggtgta tagattattc
    20521 tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg cgcattgctg cttcaacgtt
    20581 agctgcgttt aggtcttcca tttttaattc agcgatttct cttacttgat cgctagaaac
    20641 tttcgcaact ttatttttgt ttggttcgcc tgaacctttt tcgattttag ctgctttttt
    20701 caatagaaca gcagctggcg gtgtttttgt aacgaatgtg aatgaacggt cttcgtatac
    20761 agaaataaca accggaatga tcaaacctgc ttgatcagct gtacgagcgt tgaattcttt
    20821 tgtgaatccc atgatattga tacccgcttg acctagtgca ggacctactg gtggagctgg
    20881 agttgcttta cctgcaggaa tttgcaattt aacgattttt tctactttct ttgccacgag
    20941 acatacctcc ttaagtccgt gatgtggtta attggagatg catatttctc ctcccacgtt
    21001 ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa caatgcacac tggtacagta
    21061 tagcaaattt atttgttttt tcaagatttt tttctgctat ttttacattt tgcttgaata
    21121 aacgctaaat ttattctaaa aaaacaacaa tttttcgttc tcatttcttc ttttatgtta
    21181 cacttgcgct agcatggttc aacagatgaa agcaaacaat aacacggttt tttagaccct
    21241 tgtacaaaga ttcctacata acaaaatgag gtggagaaaa tgaaagattt aaggtataga
    21301 agtgtctttg atattattgg acctgtgatg attggtccaa gtagctctca cactgccgga
    21361 gctgctagaa tcgggaaaat catacgtagt atatttggcg aacagcctga ttctgtagat
    21421 atttatctat atgaatcatt tgcaaaaacc tatcgggggc acggaacaga tattgcatta
    21481 gttggcggcc tgttagggat ggaacccgat gatgaacgtt tagctgattc tttgaaattg
    21541 gcatatgaag ccggtatgga agtcttgttt gttcctaaga gtgaaaaagc cgaacatcca
    21601 aactctgtaa agatgcttct aaaaaaagga acacgaaagc tttcagtcac tggcatttcg
    21661 atcggcggtg gcaatatcca gatttctgaa ttgaatggat tcaaaatttc tttaagcatg
    21721 ggtactccta ccttgatcgt tgtccaccaa gatgttccag gtatgattgc taaagtgact
    21781 agtatacttt ctgagaccaa tacgaatatc ggcactatga cagtgacacg ggaatcaaaa
    21841 ggtgaaaatg ccattatgat aattgaagta gatgatcctc aagtagaaga aacggtaaaa
    21901 agactcaaac agctgccaaa tatcgacagc gtaaattatt tcgattaagg agctaaaaaa
    21961 atgtttttat cgattgaaga attagtagat caagcaaaag cttatccaag tgtagcagaa
    22021 ttgatgattg cagttgaaat ggaaatgagt ggccgttccc gtagtcacat catcgagatc
    22081 atggaaaaaa acctctcagt aatggaacaa tcaatcaacg agggaaaaga aggtgttacc
    22141 tccgtcacag gaattaccgg tggtgatgca aaaaaattag atgattattt aaatgaggca
    22201 gactttttaa gtggagaaac aatcttaaaa gctgtacgca atgctgtagc tgttaatgaa
    22261 gtcaatgcaa aaatggggtt gatttgtgct acaccgactg ccggaagcgc gggtgttgtt
    22321 cctggtgtgc tgatggctgt ccgagatcgg cttgatttga cacatgaaca gcaattggaa
    22381 ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa acaacgcttc tatcagtggt
    22441 gcagaagggg gatgtcaagc agaagttggt tctgcaagtg caatggcagc agcagctttg
    22501 gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag cagtagccat tacattgaaa
    22561 aatatgatgg gactgatttg cgatcccgtt gcaggcttag ttgaagtccc gtgtgtcaaa
    22621 cgtaatgctt taggctcttc tcaagctttt atctctgctg atatggcatt agcgggtatc
    22681 gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt accaagtcgg acgtcaaatg
    22741 ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg ttacgccaac tgctaaacga
    22801 cttgcggaag agattttaaa taagagggcg tgataaagct ccttaatttc gagtattaag
    22861 agattagtta tcttcataac aaaaaacgaa gagaggacta attgcccttc tcttcgtttt
    22921 ttattaatcc atcaatttcg tatattttac ttcatcgatc gtttttgttc ctgcaagttg
    22981 catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca gatgttactc cttctgcccc
    23041 acctagattc aacccataaa tcactggtcg gccaatcgca actaagtcag caccacttgc
    23101 cagagctttg aagacatgct ctcctcgacg gacaccagag tcgaaaatca ctggtacacg
    23161 tttgttgact acttcggcaa tttttggcaa tacttcaaag gacgcaggac ctccatctag
    23221 ttgacgtcca ccatgattgg atacccagat accatctgcc cctgcagaga tagcgattac
    23281 tgcatcttct ggcgattgta ttccttttac gataactgga agatgggtga tttctttgat
    23341 tgttttgatg tcatctggtg tcaagccttg ttttgcagct gcataaattt ctgcgattcc
    23401 ttttccttcg ccattaccac ttgcactttg ttcgctatac gcagctaagt taggcattgg
    23461 taatggaaat tggaattgat tgatgacgtc ttcttcacgg taaccaccga gcgttgaatc
    23521 tgccgttaaa atgatagctt tagcccctgc ttctactgct ttgttcaaaa tgaactcatt
    23581 gaatctgtcg tctttactca tatataattg gaaaaactgc ggtgcgcctg gcgctgcatc
    23641 ggctgcgtct ttgattgtcg tatttgcata agtgctgatg gaaaagattg atccagcagc
    23701 agctactcct ttagctgtat cagcttcccc tttttcatgt gccaatcctt gggccgctga
    23761 tggcgcttgg ataatcggtg tatctaattc aattccgaaa acagatgtat gcaaatcggc
    23821 tgagtcgatt ccgcgtaata tccgtggcat gattttcttt gtattaaaag aagtcgtatt
    23881 ttctttcatc gtccactcat cttcagaacc accgcgaata tagccaaaag ctcctgcttc
    23941 catgcgctct tttacacgac cttctagtta ggcaatgttt acaatatcga ttggatgttc
    24001 ttcattactt gtatgataag tcatagtaga tccttctttc atcttcttga ttacatataa
    24061 atcatactaa acttacaaaa agtaagcaaa catttactct cagatacttt tatcgctatt
    24121 ctttccaaat aaatcgactt acaagctgat ctacttcttg gttttcatga agcatactat
    24181 gagaggcatt ttcacctgtc actacttttt cctctatttc atttccgcgt tgcttcaaca
    24241 acgcatagac cgctaatgca ctgtttaacg gtactgttcc atcactcaag tctgtttcat
    24301 ctagttgacc agcaatcaag aaaaatcgag tggttatagg aatattaccg atcatttgct
    24361 ggtaatcttg atagcggtta gaaacgacag caggcccctt tgctaactca tctgtcagag
    24421 actgcgcact atcatcgaca aaatcattaa aaggcgcgcc aattgctgtt aatgtctgaa
    24481 cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata gcgtagtgca ctaactcctc
    24541 ccatggaatg ccccactatg ttgactttat ctatgccgta gttttctttc agatacaaaa
    24601 gacaagtttt gatccattct gcttgattcc actcgttatt tttattatct tcaaacaata
    24661 cttggaccat cggattgtct gcttttccag acaaatctcc tgttgcttgt atttgtccat
    24721 ctgttcctac tgttaaaacc aattctttta ctgcttgctg atttttttcc aaacgccgca
    24781 acatcccttt aaaggaattt tctgtcccgc tgtagccgtg gaaaaaaagt gttggaatag
    24841 tggttttttc tatcacttta ctggattctg ccgttttttc tttatcagaa acttcagtag
    24901 tatttatctt cgtcgtctgg caaccactta acgcaaacaa actgataagg actaaagctg
    24961 ctgcagttgc acgtttgcat aaatttttga ttggttctgt catacacctc ttcctttcca
    25021 tatagaaaaa agaaagactt gaaaggccaa tcatatgatc gacatttcaa gtctctttat
    25081 taatcgattt tatctacttg atcgaaatca agttctgtac tggtttcacg tccaaacata
    25141 tcgatgttga ctttcaattt ttctttttct ccgtcaactt ctgttacttg tccttctaat
    25201 ccagcaaatg cgccttcgat gattttcacg acttcgccaa cttctacttc tagatcattt
    25261 tgtcgcaggc tcataccgat agaacgcaag atatgattga cttcttctgg aagtaatggt
    25321 gctggtttgc ttccggctcc gtgtgaacca acgaatcctg tcacaccagg tgtgttgcgg
    25381 acaacatacc atgaagcgtc tgtcatgatc atttcaacaa gtacgtatcc tgggaatgtt
    25441 ttatgaacga tttctttttc tttcccattt ttgacttcag tttctttttc ttcaggaaca
    25501 acgacacgga aaataaagtc ggccattttc atactttgcg cacgagattc gatatttgct
    25561 tttactttgt tttcatatcc tgaatatgta tgcaagacat accagttttt ttcaaaagat
    25621 tccattgaag gctcctcatt tcattgtaaa ataaaaaaac ctcagtacac ccgaagtttt
    25681 tctttcgctt cattatagca ctaaaagtac gttgtggcta gcgagttatt ttaaaatcca
    25741 gccaaataag ctttggatgg ctgtatccat tacgtaaaac atcaatccga aaataataga
    25801 agtttcgatg actactaacg tatctttacg gagttgtttt ctgcttggcc aagttacttg
    25861 tttgatttca tcttttacgc tttttagaaa tttcatgaga caacctccct tgctactgtt
    25921 tatttcgttt ctttatgtaa tgtatattga ttacaatatt tacaaaactt attgatttcc
    25981 aaacgttcgc cgctttttcc ttcactgact gattttgtgt agttgcgaga cccacaaacg
    26041 gaacaagcga gtgctgcttt tttctttgcc atttacttct gcccccttgc tgagaatcag
    26101 acttatactt cataacagta tcacgtaact ttttacttgt caatttgtga aataaactat
    26161 ctattgtttt ggataaaatt atgatatgat taatttgaaa ttaagctaaa atgagacaat
    26221 tggaggtata gcgatgctat taaaaacact tgtatataag aaaaaagatc tgacaaccgt
    26281 tacagagact agtacattag aagaagcatt aaaaatttta gaaacttccg gataccgttg
    26341 tgtgccgatc ttagattctt caggtaatat tttccgtgga aacatttaca aaatgcatat
    26401 ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct gtcactcatc ttttgaaaaa
    26461 cgctacaaag ttcatttacc tggatacttc tttcttcaaa gtattcttta cgatcaaaga
    26521 gcttccttat attgctgtcc ttgacagtga aaatcatttt tatggtattt tgacacatag
    26581 tacgttgctt aatatgctct ctcaatcttg gagcgtagaa caaggaagct atgtactgac
    26641 aattgcttcc acaggaaagc aaggagattt ggctgctatt gcaaaaatca tttcaaaaca
    26701 cagcagcatt gccagctgta ttactcttga tatcgaaaaa gacgaataca ttcgacgtac
    26761 attgatcacg ctaccggcta acactgaagc gaaagtatgt gaaacgatcg tcaaccactt
    26821 agagaaaaag aattataaag tggtagaaat tgaaaatttg caaaaccaag aagcataatc
    26881 aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt tcgcttttta taattagaca
    26941 cgcttttcaa aatcaagcgc gcgatctaaa taatacatca atggtgctga aacagccatg
    27001 acgataaact cacttaatgc aagcgtgcca taggttgtcc aaaatgcttg gccacctgtt
    27061 ggaactaaca tataagcaat cagtcccatg ctgatggtaa agaagatcgt gttcaatact
    27121 aaacgcatct ttacattttt cactttgttt tgcaataaag ctgtcaggcc taaagaaagc
    27181 aatgtttggc cgccaccaaa aaccacatcc atgataccaa aaccaaagaa ggcattatag
    27241 acgatgaccc cgcctaaaac tccccaaagc aatttacggt taaagacaac taaatgattc
    27301 aaactttctg aaatacgaaa ctggatcggc cctgaggata caggcgcaac taaaatagtt
    27361 aacgctagat aaagagccat gatgatgcca tttacaacga ttacggataa ccgttgtctg
    27421 cttttcgttt tttgcatttt ctctcctctt ttctcctagt ttttttaacg cgggatggtt
    27481 gatgaaccgc ggttatgtga aacaaacaat aattataaca gacttttcat ttatttcaat
    27541 ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat gtaaacgttt gctaaaaatc
    27601 gtgggatatg gtataaagta agtgtaaggg gtcataagaa ttcccagtaa agcagacgaa
    27661 gtaggatctg caagaacaga attgttgtga aacttaaata aataaaaaag gatgtgggaa
    27721 tttgagcaac ctcttactag agaacggtct agcaagcagc tgaagatcta agtggacatg
    27781 ccgaaccttg gaacttctcc ttacgtaagt aatggatcat gcgcttgcta ggcttttttg
    27841 tgtttttcgg tcttcggacc tttttcattt tcctagactt ctgctataat aaccaagaat
    27901 taattttgta acagattttt ttgaaaaaac aagtttcttt cattcaaaaa atagaaaaag
    27961 cttgctaatt attggttaac cagcaaacag gagtattaaa atatcttgtt ttattgaata
    28021 gcaggaggaa atcatgaaat tccattatca tatattgatc aatccatcgg caggcagcgg
    28081 aaatggctat aaagtagcag aaagaatttt accggtatta aaaaataagc atattgacta
    28141 tacacttcat tatagtgaat acaaaggaca cgatgctgag attgctgaaa ctttggcaaa
    28201 agaaacattg attccttgga tagaagaaga acagaaaaca atcgatactt tccccctttt
    28261 gatcatcgta ggaggtgacg gcaccttaca tcaggtactt gatactttct atcagatgga
    28321 agtagaattc ccagttgctt atattccagc aggttctgga gatgattttg ctcgaggggc
    28381 aggccttcca aaaaatccca aaaaaggact tcagctgatt ttagcagctc aatcaccaga
    28441 aaaagtccac ataatggctt atgaagaaaa aataagtgaa aaaaaaggaa tagctgttaa
    28501 taatttcggt ataggattag atgcagctat cgtccatgcg acaaatcatt cttccacaaa
    28561 aaagcgattg aacaaataca acttaggatc attttcgtat ttattctcta ttctccgtgc
    28621 tttattcact caaaaaggat ttccaattct ggtagatgca ggaggaaaac aattaagttt
    28681 ttcaaacgca tttttatgta cagcaacgaa gcatcctttt tttggcggag gaatcgcgat
    28741 tgttccaact gctgatgcgt ctaaaccagt cattgatttt gtcgtggtcg aacgaatcaa
    28801 tttctttaaa atcttgtggc tcattctttt acttatacag aaaaaacaat tgaaatcgaa
    28861 atatttccat cactacacaa caaacagatt acgtatcgtt tcaacaacac cgcaacatgg
    28921 tcaagaagat ggagaagaaa tggaaaaacg accttttgat atcacaataa ccacaactaa
    28981 acagttgttt tggtgttaat aaaagaggtt ttgtcagaag ctacatctgg aacaccgttt
    29041 attcggaaaa aaggactgtg acacgacttt tgtcacagtc ctttttttac tatgcagtta
    29101 attctttgcc caattgggtt gccatcgata gtttctttct ttacccaaaa taaattggat
    29161 cattgaaaac accgcagcaa aagcaactaa gacacaacca ttgaaccaaa aagattctgc
    29221 aaatgtttga ttgtaactga gtgtttcact gatcaagtta ttattttgaa caaaccaata
    29281 tgttggtgtg aatgccgcta ttttgctaac catttctggc aagtattctg ctggtacaaa
    29341 caccccaccg ataaagcaac tgcctgtgat aaaaatatct ttgatcccac cgctggtttc
    29401 tgagtttttt accaatgaag tgatacaagc cgaaaagcta atgatcggca ataaaaacaa
    29461 gaaagtattt aaaatatgat accataccgt tatatcccac gaggtgtgga tcactaacag
    29521 aaccattaga agtaagaagc cactgaacaa cagcagcgag taactaatca aactaaaggt
    29581 tactttgcga ttcaagtttc gtttggtaat gggcgcactc tggtttcgtt tactgatctc
    29641 tttgcgatta aatgctagat tgatgacgct gaaaccgcta aaaatggata gaaacaaccc
    29701 ataagacaag aaattaaagg ttgtgcccgt caacagcttt ttcatccgct gactatatcc
    29761 cgcacttagc tcaacttctc cagtaaccga gagtgccatt tgtgtctgtt ccatagctgc
    29821 atcgattgtt ttatcttctt cttgaaacag aatcagtgtt tgcaaaaagg tgttcacttg
    29881 ctgtgtaaca actgttttag aaaaggcatc tgggcgactc tgtatcttga tcgtcggcat
    29941 ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga tgtaagataa tgtttacacg
    30001 attaaaataa agagcatcat cgatttcttt ttgtgaggtt ccatccaatg taacgactgt
    30061 ttccttgtca ttcaaatatt tacgaaggcc atcgactaca ggcgactctt cttcactaat
    30121 caaggcgatt ttcgcatctt gcagagaggt attttcaaca tcaatatttt gcgcaaaaaa
    30181 gaatgtggca acaacagtga taattacacc tatcagtaaa ttcgatttat ttgctttaag
    30241 caatgtccaa atcgttttat aaagcgtcat aacgcacccc tctttccatc caagcatttc
    30301 ctaacgcaaa aaggaaaata aatccgatca accatagaag attggtataa aaactagcca
    30361 aatcagtgta gaaataaagt ttatacaaac tttcgctgat caagttgacg agatttaatt
    30421 gaccaacaat cggaatatga agatcaatcc aatacttcac ttgttccgac cccatcatac
    30481 cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat gctatccttt tgcaccagat
    30541 tcatttttgg tatcaaatta ccaaccaatg tacctaacaa aatcgcacac aacgacccca
    30601 gagcggtaac aatcatcaac cattgccaac gttcaccaaa gtctacttga tagataaaac
    30661 gaaagacagc taacataata taaatctctg tcaaaacaat cgtaaagctt gctgccaaat
    30721 tagcgattga aactaacgat tgctttctag gaatcaagga caagcgaata ccatttgacg
    30781 attgatttgc ttgctgatcc tgtacattgc ggacccccca catcgtcccg tagagaatcg
    30841 ccattccaat cagagtgaag aaataaaaac ttttgaaact catattacga cctgcatgaa
    30901 cttcttgaaa agatacttct tgttcaaaaa cagtcatgat gggcgcgaga tcaacgttct
    30961 ccacttctgc cactttactc gaaattatac caatacgctg taaaaattga tccatgacgt
    31021 ttttcatcaa cgtctgactc attcctgctt gacccacatg gagcgaaaca gatgaaccat
    31081 caatgagata aaaaccagcc aaatcatctg catttagctt ttcattcgct tctacttccg
    31141 atagttcctg tacttcaaat aatggtttat cattgacttc gacttgttct aagactgtgg
    31201 taaaattttc cgaaagcgct gtttgatccg ctttgattac aattccagta ttaatcgttt
    31261 cgagattgcc accttgatca atctcgccaa aggccgccat aaacatcaat cctaaaagaa
    31321 ttggaaaggc caatgtccaa aacaacatgg atttatttcg cagcagtatt tttgtgcgat
    31381 atttataaaa atgccaaaac attatttccc ctcctctagt ctctcaaatc cttgccagta
    31441 atctctaaaa agacatcatt caaggacgct tctttggttg ctatttttcc gtaactgatc
    31501 ttttcttctt gtaagattgt aagaatcggc aacaatactg tatttagttc agtagttgtt
    31561 acttggagct gacctttttt ataagcgatt ccatcagaag ctattgttcg ctgacaacac
    31621 gttttctggg agtaatggga tgtcgatcgt caaacgatcc gccgtccgta ccatatcttt
    31681 tagagattct ttggttcctt tagcaatcac ctgtccttga tccatgatca cgatctggtc
    31741 gcaaagaatt tctacttctt ccatgtaatg tgttgtatag acaatggttg ctccttgtcg
    31801 attcagctcc ttgatacttt ctaaaatttt attccgactt tgggggtcta cagctactgt
    31861 cggctcatct aaaaaaatta accgcggctt gtgggcaatc ccacatgcga tgttcaaccg
    31921 acgtttcaag ccaccactaa gttctcctgg acgaaacgaa acatattttt ctaatcccac
    31981 taattgaatc acctcttcaa tcaaggcttt ccttttctct ttttcggaaa catataaacc
    32041 gcaaaaataa tcgatatttt cttgcacact tagttcgtca aagaccgcaa cgtcttgagg
    32101 aacgacccca atcattgcct tgacagcata attttcagga gtcattgttg tcccgaacaa
    32161 tttaatggtt cctttgtcat aatttaacaa agaaagcata cagttgatgg cagtcgattt
    32221 cccactgccg tttggtccta acagacctaa gacttctcct tcttgtactt gcagatcaaa
    32281 atgattcaat gctacaaaat ctccatagcg tttgactaat ttatcaattt cgataatcat
    32341 catgaaaaac tccttccaaa tttcttgttg atctgactat aataaagtgc aagaaaagaa
    32401 actagtgacg attggctaaa ccacacatga catttgtcac aatgaaggag tctgctatgc
    32461 ataaatctcg gctactcaac gaaactagct ttctactggt tatctcattg cttttgctgg
    32521 taagtcaagg attcgttgct atgcaagtta tctatttatt gatcgccgtt ttattcactg
    32581 gtgtcagtta tttactgcct acaaagccaa attggatttt ccaagttatt ctatttcttt
    32641 tatgtgcctt tttacctgat cttgtcctgt ttctgccagc gacgatgcga gtgatcgccc
    32701 ctgaagaaag ccaccttgat tacttgtttt tgttaggttt tttgttgatt tcgacggttg
    32761 gtaatcatcc ccccatcaag gtcgcctttg tcgtaatttt gctgtttttt tccttttact
    32821 tacgaaaaaa agatcaacga acgaccgatc ttgagaaaaa gtacattgtc tccaaagata
    32881 ctcattggga aaaacaacaa gagttatcgg aacaaaataa agcattggcc ctgtcacaag
    32941 aaacacaatt agccttgcaa attgtggaag aacggaaccg aattgcccga gacatccatg
    33001 acaatgtcgg tcatctgctg tcaagtgcga ttttacaaat tggtgcgctt gaaatgatca
    33061 atcaagaacc aaaactaaaa gaaccattag aaaatttaag tgccaccgtt cacactggga
    33121 tggatcggat tcgtcaaagt gtccatgacc tccatgagac atcgatctct tttcaacact
    33181 ccttagagtt tctgatcgct gattttcctt cttctgttac gattgaagga aacttgtttg
    33241 aaggattgaa tgaaacgcag caaaactgct tcttaatggt catcaaagaa gccttaacca
    33301 acagtatcaa acatagtcat gccaaaaccg ttgttttaca ttttcgaacg ttgccggcct
    33361 tttatcgctt gcaaatcacg aatgatggca ccagcccaca aaaaaattct gctcccggta
    33421 ttggtttgaa aacgatgcgt caacgtgttc aagaacaaaa gggacaagtc catattttcc
    33481 aagaaaacca gcaatttcag ttaacgatca ttttaccaag ggagggacac caatgataac
    33541 agtagtcatt attgagatga tccatttgtg acgaaatcgt tacaaaccat tttagaaagc
    33601 acgaaagaga tccgtgtgtt aggcattggt cattgtgcca aggatgcgct agtcttatac
    33661 gaaacccatc gtcccgatgt tttgctgacg gacatccgca tgcctaaaca aacgggcatc
    33721 gatgctgcca aagaaatttt ggctaaattt cccaaagcca tccttttgct tttgacgaca
    33781 ttcaaagatg aagaatacat ccgcgaagca ttttccatcg gtgtcaaagg gtatttaatc
    33841 aaacagaact tgcaagcgat tattccttct gtcaaatccg cttataatgg acaggccgta
    33901 tttggtaatg aaatcatcga gactttcact caaatgatga agaataaacc gacgattgat
    33961 agtcatgctt atgcttcttt tagtgaacgt gagcttgcaa tcatcaaaga agtagctgcc
    34021 gggaaaaaca ataaagaaat tgcagatgcc ttgtacttga gtgacggcac tgttcgcaat
    34081 tacatcagcc aattactaga aaagctagat ttacgggatc gcacgcaatt ggctatctat
    34141 ttttatcagc atttgcaata agtcttatct aacacaaaaa aacctgaatg atgctccttc
    34201 gcatcattca ggtttttttc acatcttcta ttcaattgtc ccgcaaacca tttcctcacg
    34261 attgaaagaa tcaaggattc gcagggctgc caggattcgt tggattgttc ggcgtgttag
    34321 gcggaaaaat aggcgccttg tctaacgttt tttctagttt tgattcttca cgatcgtctc
    34381 gttctacatc aggactttca ttcaatttga attctttttc catctcatct aactttttaa
    34441 agtcattgcc agattctttt gctaatttcg ctaatttatc ttcggtttct ttagccataa
    34501 agtaacgccc cccttttgat ttcagcttag catatcacac tgaaaaaaca aaagaaaacg
    34561 ctaaccaacg atcattttta gctatttctc atctttaaaa tcaagcattt tttgccggta
    34621 taaaatcgta gggagaactg ttttccatac caatcatcgg atctactaac tggttatcga
    34681 tcacttctgc tgtaagaacg aaatctttag aaacagcagg ttctacctct tctttctttt
    34741 cttctccaac aaatgaagaa aacagatctg gttccactgt ttccattact tggtttttcg
    34801 tttcattcgt cccactgtca tcttctacag gtagcttttc ttttacaacc cgtggctctt
    34861 ttgtatagtt cccatcttca aatggatcat ttccgaccaa agaatcttca taagctgcta
    34921 gtttggtccg gagagtgata ttcattttat cattatccat gatccttgat ttcaacgtag
    34981 tcatccggct tgctgattca ttcgctacac aagtgacata ggcttgctct tcccctaaca
    35041 aaatcaacag ttccttactg cgagtcactg ccgtgtagag caaattacgc tgaagcatcc
    35101 gctgatattg atggaccatg ggcaaaataa ccatcttgaa ttcactgcct tgtgatttat
    35161 ggattgagca acaataagaa agagtgattt tattccattc gtttctttta taagtgacct
    35221 catttgcatc aaactggata actaattcat ccactttatc ttcagaatct tttgccaaga
    35281 tgatacccgt gatttctccc atatctccat tgaatacatt caattcggga gtattgacta
    35341 attggaggac tttgtctcct atccgataaa cggtgtcgtt ccaatgtact tccttttttc
    35401 caggtttccc tggattgaat atctcctgca tcattttatt taacgcatcg atccctgcag
    35461 gtccccgata cattggtgct aatacttgga tatcttgtgc acggaatccc ttatttttcg
    35521 ctttttccac aattttagag atcaagggtt caatttgata agcatcactt cggaaatagg
    35581 aacgatcttt ttgattcttt gtgaaatctt ttggcagacg tccttctttg atctcatggg
    35641 caagaggaat aatactagaa ccatcacctt gccgataaat ctgattgagt tccattttgg
    35701 gaatttgttc gatttccaaa agatcatgga gtacttgacc aggaccgaca gagggaagct
    35761 gatctttgtc tcctactaag ataacttgca tattcgtcgg aatcgctttg aataatgtgt
    35821 tagccagcca tgtatcgacc atagacatct catcaacaat caaaagtccg ccttctaatt
    35881 ctttcgtact gactgaaggt gttttttccc gtccattcag ccccagaaga cgatggatcg
    35941 tactggcagg cagacctgtc gtttcgttca ttcgtttagc tgctcggcca gttggtgctg
    36001 caagtagaat aggaaaaatt tcttgggtat attttgaagg atccaattcc agtccgttca
    36061 actctgcaaa aagattgacg attccgttga tcacagtagt tttccccgta cctggtcctc
    36121 ctgttaaaat aaatagaggg gaacggattg cttcagcgat agcttgttct tgcgaaggtc
    36181 catactgtat gccgaatatt ttttcgatac gccgaatatt cttctgcact tctttttcag
    36241 gataatggat ttctttttta cgactgagaa ggcgctggat cgaattaccg atcccccatt
    36301 cagaaaagta caaactgttt tcgtaaagat tagtctcttc ttgttgtatc tttccttctt
    36361 caacaagttg gataattaca gtagcaacct gatcaggctg gatttcaatt gggcgacttg
    36421 tttctaaaat acggatcgtt tgctgaagca atgtctccgc cggtacgtat gtatctcctg
    36481 tttgaactgc atgctgaaaa atctgatgca agatagctgc tcgaattctt ctcggtgaat
    36541 ctgctgcaat accgatttgt tcggctagat tatctgcacg tttgaacccg attccttcta
    36601 tgtcttcgac tagctggtac ggattttctt cgatgatatc caaagactcg ttcttatagg
    36661 cctggtagat actgaaagct aactgactac cgaacccgta acgattcagt cccatgatca
    36721 cttgatccat cccgtggttt agccgaatcg tctcagcaag catttctctt tttggcttcg
    36781 tcaaaccgga gatttgtttt aataccgaag gatcagcaat gattttttcg atagcatctt
    36841 ctcccagcaa ttctacaatt ttttctgctg ttttcttccc gattcctggg aatttttcgc
    36901 tggaaagata cgctacgatc ccatttgcag ttgttggctt ttcttgctca tatctttcga
    36961 gtttaagctg ccttccgtat ttaggatgat ccactaattc tccataaaaa cgataagtct
    37021 cattttcttg aatatcgccg aaacttccgg tgactacaat ctctttttca cggtaatcag
    37081 ctgtggtttc actgatgtgt accaaaagca ctttgtaaaa attactagga ttttgaaaaa
    37141 aaatcgcggc aactgtacca acgaaataag gcatttcttc tggctgcatc ggaagccgcc
    37201 tccttcttct atagtaaagg ttctggtgct gtttcagttc ctaaaaaatc tgtttggttc
    37261 catactttca gttcatatgg caataatctg caaggctctt cagtctctaa aatcgtcaaa
    37321 ctactattga acaatccacc catttcacgc aactcactta gctcttttcc tgtcatccat
    37381 tgaatggctg cagttaaaga agctccatgt cctacgaata aaagtgggcc atctttatgt
    37441 tgggcaaccg catctgctac aacagtttcg atacgtgtga ttgccgattc aatcgtttct
    37501 ccgtgaaaag gagaaggatc atacagatct aaatgattac gcaaattcgg cagattatcc
    37561 ggatatttct ggtacatttc atcgatcgac tgaccttcta aagtacccag ccctagttct
    37621 cttagcttat ctgtataaaa aatctctacc ggacgagcaa gctctttgtt gataccttct
    37681 gctgtatcac gtgctcgctt tgctgtactg gaataaattg ctttaaaagg aacatatttt
    37741 acctttttcc caaatgcttt gatttcttcg taactttgtg gcaaaagcgg agaatcgcca
    37801 ttgctccctt gaaaacgtct ttcctgattc cattgtgtct tcccatgtcg ggtaaaatat
    37861 agttccatac gcagcaaact cctgtctttc ttttatttta ctctataata aactgtctaa
    37921 tccagcttgt cattttcata gtgatgcgtc aattctaaat tagggaatcc ttgctggcga
    37981 agtgcttcat acactatcat tgcagctgta ttcgataaat tcaatgagcg gacatgctca
    38041 tcattcatag ggatgcgtaa acacttttct tcgttttcac gcataaatgt ttctggcaaa
    38101 ccggttgttt cttttccaaa taaaaagaag tgctcttctc catcagcaaa attttcgtca
    38161 ctatatactt tatgggcaaa ttttgagatc aaatgtagtt tacgattacc taaaaatgtt
    38221 aaaaaagctt ctaaattact gtggtaagta atatttacat cattccaata atccaaccct
    38281 gcccgtttta gatgtttgtc atcagttgaa aaacctagtg gctcgattaa atgaagcgga
    38341 gtattcgtgg ctgcacacgt acgggcaata tttccagtgt ttgcagggat taatggttca
    38401 aataaaacga tatgattcat caggatcatt cctctcatat ttatgttcgt ttctattttc
    38461 tgacaatttg atttttgcat aaaaaaaaac gtatcaactc ggtgtcagtc actgcgagtc
    38521 gttacgttag tgaagctctt ctcactaact tttatcagaa caggaacctg ataaaaacca
    38581 atgaaaaaac aacttgttgc taatataaca ccctcaactt ttttttgcaa cctctactca
    38641 ttaaaaactg ctagaaaaac gttttctctt agttcctttt aataattcta tgtcaattgt
    38701 aatcttcttc aaaggattct tatataagtc tgctttgatt cgctcttcta cctgccattc
    38761 taaaggtgac atggccaaaa gatctttttg attttcctct ggtatctcaa attcataggt
    38821 tacacgctga cgttcagtta atttcattgc ttcagcaaac ttggctacta cacgttcatt
    38881 ggaataactc tgtttttcag gttgattcgg ataaaaagcc tgacgtagtt cctttaaata
    38941 attttcttgt ggcaccacct tcaacacttc tccatcttct gagagtacac gttggaattc
    39001 ttggtaatga gaaggcgaaa agatatttaa aaccgcgttg aatgtttgat cagcaaacgg
    39061 caaattggtc aaatcagcta cacaccaaaa tgcttcggca gattgttctg tagctgcgta
    39121 gactccttct tttgaaatat caaatccaac agcattttga atctttttca attttgaaag
    39181 ttcattcaag aagctacctt ctccgcagcc aacatctaat agcgagactt cttcaggtaa
    39241 atgctgcgca atagtctcaa taacgggctg atacatgcca gattggatca ttctacgcct
    39301 tggaacgaac attccttgat cgtattctgt tttaaccgca tgggataaaa aataaagtgt
    39361 tccttttttg gaaaggtcaa aacggtgatt atttttgcat atcaagccat tatcagactt
    39421 ttttacttct tcttggcaaa ccggacagcg gaacagctga tgattttctg ccaaaaactg
    39481 gcgtcctcgg tcaatttttt ttaacatagt tccccctcat ttctgtttta tcttaccgtt
    39541 agatgctcac tttggcaact gctaaaaata tgatacaatg cctagaagaa agtgaaagat
    39601 aaaggagaag attagatgat caaagaattt tgcgctgaaa actacacatc tattcctcta
    39661 gcaattgcta gaggagccga tcggatcgaa ttgtgtgaca acttagcggt cggtggcaca
    39721 accccgagta ctggtgtgat cgaagaaacg ttaagttatg ctggggaaaa agatatcccc
    39781 gtaatgacga ttattcgtcc tagagggggc gacttcgtct ataacgatat cgaattgaaa
    39841 atcatgcata ctgatttgat tgaagcaaag aaattaggga cggatggcgt agtatttggt
    39901 tgtcttactc cttcaggctg gctagacgaa gaggccttag aactattgat cgaaacagca
    39961 gaagggttgc agatcacctt ccatatggct tttgacagta ttcctgaaga tcggcaattc
    40021 gaagcaatcg attggctggt agaacatggt gttgaccgta tcttgactca tggcggtcct
    40081 gctgacaaaa ctattgtaga aaatttccca cggcttaaag aattgattgc ctatacagat
    40141 ggccgcttaa tcattttgcc tggtggtggt gtcactgcag agaatgcagc acatgtagct
    40201 aaagaattga acgtaagcga agtacacgga accaaaatcg tggatcttca gttataatct
    40261 tataaacaaa aaaacatttg caacaggcat aatgcttatt gcaaatgttt ttgttttttt
    40321 acataaaata gcccataaat aaagaagcaa tattgaaata aatcagtaca ctcgtcaaat
    40381 cgctcaaagt cgtgataaag ggaccgcttg ctactgctgg atcaaatcct agacgatcca
    40441 ttagcatcgg gattaaacta cctgcaagat tcgccactgt gattgcaaat agcatagcca
    40501 tccctatgac aaaccccaag ataaaattat gtttccaaaa acctacgatt agaaaaatcg
    40561 tgatccccgt aacagctcct gtgacaacgc cggtcaagac ttcagccaaa acaagtcgaa
    40621 caaatccatg atctttctca tcagaaatcg caagtcgacg aacagccaca gctagcgact
    40681 gggttccagc attaccggca gttcctgtaa tcagcgaaat gaagactgct aaaatactcg
    40741 cttcacttac caagtcttca tagtgactga ttaaagaagc ggtcgacata cctaaaaaca
    40801 acagagtgat cagccaaggc aaacgtcgag acgctgcctt tactggattt tcattaattt
    40861 cttccacatt gaccccggca agtcctgaat aatcgctagc agcttcatca tcaataacgt
    40921 cgatgatatc atcgactgtg acgattccta acaaatgatc atcataatcg gtaacaggaa
    40981 gagctaagaa gtcataatct ctaaatgttt gtgccacatc ttcttggtcg tcgcctacat
    41041 gaactgaaag cactcgttcg ctcatcagat cagaaatcat tgcgtcgtca tcattcacga
    41101 ttaggtcgcg cagagaaata acccctacta gctgaccttc ttgattaacg acatagatgt
    41161 aatagattgt ttcagctaca tctgcttcgt tcttcagcac gtacatcgca gagcgaacag
    41221 tttgattggc tacgatcgaa acaaattcag ttgtcatgat cgcgccagcc gtttcatcct
    41281 cgtaatggag aagttccttg atttcactgg catcgtctgc cgacatcaaa cttaaatatt
    41341 tagcaatttg ctttttatcc agcgtattaa gcaagtcaac agcattatct gtatacatct
    41401 cagaaagcat atcagctgca tagctaggac gcatttcatc taggtattct ttcatatgtt
    41461 cgttgtcttc ttcaatcaca tcgaacatat ccgctaattc tttaggcgac agataggaat
    41521 agatcagctg cctgttctct tctgtaagag attggtagaa ttgcccctgc tcatagatat
    41581 gcatctctaa aaaactgttt ctgaatgcct gaatattatt ttcccttaaa tcttgagtca
    41641 actgggcaaa tctttcctct aattcttgat tttcatccaa accaatctca cctcgcctta
    41701 ttcttcaaac cgtctcacaa ctgcctccat gtctgcggct aaaggctgct tgaactccag
    41761 caactcttct gtaaatggat ggtaaaaacg cagatcataa caatgaaggg cttgtctttt
    41821 tattcccaga tccattcgcc cgccatacat atcatcaccg agcagggcac agccaattgc
    41881 ggaaaaatga actcttattt gatgtgtcct tcctgtatgc agctggatat caactaacgc
    41941 gatttctgtt agtcgcttct ctatccagta ctcagtctgt gccgaacgtc cttcatcaga
    42001 gaccattcgc ttcaatagcg aagaataatc tcgggcgatc ggcaaatcga tatgtccatg
    42061 tgccgataaa tctgaaagcc ttccactgac taaggcctga tattttttca caaacttctt
    42121 ttcacgaagt tgcatatcta attttgcgtg cgcaaatcca tgctttgcaa aaagcatcaa
    42181 accagatgta tcccgatcta gtcgcgtcac cacatggatt acttggtttt cgtaaccttg
    42241 ttttttataa taagccttta cacgattagc cattgtccca tttggatggt attgggcagg
    42301 aatagacgaa acgcctgctg gtttatttac taccagaacg tgttcatctt catacacgat
    42361 atcgatcggt gtttcatcca aaagtacggt ttcatgttct ccttcagcag gaatcacaat
    42421 ggttacctta tcatccttag ctagagaaaa caagacattt tctacttgat cattaacttt
    42481 gatttgaccg ccttgaaatt ttattttcgc taacagaccc tttgagatcc ctttttcttt
    42541 taaaaaatat tttacttgct gagaatgatc tttctcataa tcccatgtaa actgcatatc
    42601 aatcctcact aataaaggca tctttcacac gatgccaaaa atgcatatgt cgataagaag
    42661 caaaatggat ccgttcttct gctattcgat aataaattga gcggatattt gcttttgcga
    42721 cattcaattg atcaaccgtt acaagataat cttctgtatt ttccaatttt atttctagcc
    42781 attcatcatg ggcaatcacg atcggagaac ccaaagtacg aaacactcga ttattcagtg
    42841 aagcaatctc tgccaactga aaggcattga tactaggatg gatcacagca ccgccgacac
    42901 ttttattata tgctgtcgaa ccagtaggcg tagaaatcgt caatccatct cctctaaaac
    42961 tttcaaacag ctcgtttttg atgtatacat cggctaccat cgtacgattg gcccgtttaa
    43021 tcgttgattc attcagtgct aaaaaatgct ggtccggtgt ttcatccaaa taactgatcc
    43081 gaacatccag caagggataa ctaacacttt gttcccggtt cgttcgtaag ctgtctacta
    43141 gttcttccag ctcgtaatcc cgccaatccg tgtaaaaccc taaatgacct gtatgcacgc
    43201 ccaagaaacg cacatcattc agacaatgac tgtagtgatg gaacgccgac aataatgtgc
    43261 cgtctccgcc gactgagatg accaattctg gctcatgatc atctcgttga atccctgctt
    43321 gttctaaaag tgcgttcaaa cgttctgtca cttctatagt ctttgcttct ttattatgaa
    43381 cgatcgctac tttcatcccc tgcctcctct ctttcgcttt ctttcttcaa aagcgttcga
    43441 tagtagacat catctgattt tcctcttcca tgggagaata accgctgtgc ttcttggatt
    43501 tcttcacgga tactggacat ctcttcatcc aactgatagg cagcttctgc cgcacgctga
    43561 agtcgttcac tcatttcttc tggaaaaacg ccttgatatt tataattcag cgaatgctca
    43621 atcgttgccc agaaattcat cgcaagagtc cgaatctgga tttccgccaa aattttcttc
    43681 tcaccgctga tcagctgtac aggatactcg atcaccagat gataagaacg gtagccactt
    43741 gcttttttat ttgtgatgta atctcgttct tggagaactt tcatgtcttt tcttttacgg
    43801 atgatctcaa caacttgatg gatatcttct acaaactgac acatgatcct taacccggca
    43861 atatcagaca tttcttcttc taaacgtccc attggaatat gccgtaattg tgattttgtt
    43921 acaatactat cgactggctt gactctccca gtgacaaatt caatcgggac atgtcgattt
    43981 tgttcacgga actgtttgcg gatcccccgc agtttcactt tcaattctga tacagtttgt
    44041 tcatatggtg ctaaaaatgc ttcccaatct cgttccatag ctgatacctc tacttcatta
    44101 acattcattg tttattctac catatatgca aagttttttc gtactcttga tttatgcatt
    44161 gaccaaaaaa agaaaattag gcaaaataga caaaaaggag tcgatcaaat gacacaaatg
    44221 cttgaaatcg aatataaatc tatgctgaca aagcaagagt acgagatatt catcgctcac
    44281 tatcaattaa cagataaaga tttccgtgta cagactaata tttatttcga taccgttgat
    44341 gaacagctga agaaacaaaa ttgtggccta cggatccgtt tcgttggtca tcaagctgaa
    44401 tatacattaa aaacacctgc tgaaaaaggg cgtctcgaaa caacggatac ctttataaca
    44461 gaagaagcag aagcatttat cttcgaaaaa cgtctgcctc gttcaggcgc agtttttcaa
    44521 aaactttctg atttgaatat cgatcctgct tcattaatcc aaaccggaaa gcttacaaca
    44581 aaacgggctg aattccctat tgaagaagga cttttagcta ttgatgaaag ctggggagac
    44641 aatctacacg attttgaact ggaactagag gtaaggggat gcatctgtgg gaaaaattgc
    44701 ttttataaat tttttaaaac gtttttcttt accttatcgt cctgctaaga acaagatcca
    44761 acgtatgctt gaagctaaaa attagaaacc tttcattttc acaaggcact tgagttcatt
    44821 tttttacttt tttctgttaa gttataggat gtgaaagaat ctagtcacta gttgtataga
    44881 atctattttg ggggagattc atatgatcga aatctattta tttgttaatc ctttagggga
    44941 atactgtttc gaaatggaac aacagttatt acaatttatc gaagaagagt acgggacacc
    45001 atcaaaagaa aagatgcaat ttcgctttct tcctttggtc aatttgcaga caattgggga
    45061 tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag gaacgtaatc acttattctc
    45121 aatgacttat tctgcagcat tggactgcaa agcggcgcag tttcaaggaa agaaaaaagg
    45181 ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt aatgacatac catattctca
    45241 agaacttgct gaatccatat ttgaagaaat cggcggcgat ttgaatatgt ttaaagaaga
    45301 tcgtcagtct gacttcgtta aagaagtatt cttttctgat caaaaaattg ctcgggaaat
    45361 gggcatcaca aaacaccctt ctgcagtcgt ttacaattat gcctgcgacc gtgattttgg
    45421 tgttttagta gaagtaaaag gtacaagtag cctttggaga atcacagagc tgtgtaaaac
    45481 aacagataac tcatatcaag tttttaatgg tgaacattta catacaaatg attctcagtt
    45541 ccattcaaga actagccatt tacacttgct ttctaattaa aaaaatacgt cgactccctt
    45601 acaagagagc cgacgtattt ttttcgtttc tattcgagaa aatcaaaaat agaaagcctt
    45661 ataaacgatc gatcaattct tctaattcat ttagacgttg ttcaaacatc gacattgcat
    45721 cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt tttcattact tccacaggat
    45781 aatcgctgtt acctgctttt aggtaagcca agtagttctc tagtgcctct ggttcctggt
    45841 ttagtatttt cttcgccagt gctgaagcag cagaaaagcc agtcgagtat tggaaaacat
    45901 agtaattata ataaaaatgc ggtatccgtg accattcgaa tttaatttct gggtcttctt
    45961 cgactgctgg accataatat tttgcattca atttaccata actatcactt aggtattcac
    46021 tagtcaatgg cacacctttt tcatcttccg tatgcatgaa atgctcgaat tcagcgaatt
    46081 gtgtctggcg gaaaactgtt cctttaaacc catccaagta atggttgagt acataagccc
    46141 gtacacgagg gtctttttct gtttccaata aatactccgt taggatattt tcattcgttg
    46201 tcgaagcaat ttctgctaaa aagatggagt agtcgccgta cacataaggc tggttcgaac
    46261 gagtgaaata actatgaaca ctatgtccca tttcgtggac aagcgtaaat agctgatcca
    46321 gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc atagcttccc gaagaataag
    46381 caccgcttcg tttcccttta ttttcgacaa catcgatcca acgttcagag aatgcttttt
    46441 ctacgatggc catgtattct tcacccattg gtttcagtgc ttctaaagct ttttcttttg
    46501 cttcttcgta cgtaaaggta attggagctt cccctaagac cggtgtataa agatcataca
    46561 tgtgcagttt ttccacttct aataaacgtt tccgtaattc catgtatcga tgtaacaaag
    46621 gcaaatgctt gtttaccacg tctaccaaag tatcgtatac actttcagga atatgattat
    46681 tgctcaaaga cgcttctctg gcagagctgt aattacggac tttcgcttta aaattatgtc
    46741 cttttatatg tgtgcctaaa gtagaagcaa atgtatttct aaattgttcg taaacactgt
    46801 acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt gctttctagc aactgaccat
    46861 acacgccatg agataattgg actatttcac cattttctcc ttcaatcgtt ggaaaaacta
    46921 gatctgcatt attcaaaaca gcaaatgtat cacttgaagc atcaaagatt tcacctgctc
    46981 cagcaagaag agattcctgc tcagcagata agacatgggc tcgattatct actatttgct
    47041 ggatataatg gcgatagact tccaattttg gttcttcttt gaaatattgc caaatctggt
    47101 catctgacaa ttgcaatatt tctggttcaa accaggaaac ggcttcacta actttcgaaa
    47161 acagactgct tgctcttgca taaagcgctt ggtaatctgt atttccagtg tcttgatcgt
    47221 ttttaaggtg cgcatataca taaatgactt cagtttggcg ataaaccctc aatacgaatt
    47281 caatcgcatt taaaaattga gaagcgcctt gatcaagtgt tcctttgtgt ttttcagatt
    47341 gttttaactc ttctgataat tccaaatatt tctcatcaaa ctcttggtcg cttgaaaaga
    47401 tcttggtcaa gtcccaagtt aaattttcag gcaattcttc tcgttttggc aactgcttta
    47461 cttccatgaa atccactcct tttcttccct tagctctatc tatcaaaata gatcatagtc
    47521 taacatccac atgtcacagc atctaacttt tctactcatt catgtgtctg cttccatcaa
    47581 ttttagcata actaaatctt gttttacata gaaaaccgat tcctagccta tttttatact
    47641 tactattttt tcaaagaaga caagtgtatg cattcgttaa aaaactgctg atagaccatc
    47701 tcttcatcaa tcataggaaa aagccattcc cttttatgtt cttttatttg gcaaagccat
    47761 gagttgaaat caggaacttt ctggttttcc aaggcttctt cgtacaacat tcgataaaga
    47821 aaaacctgtt cttgtaaata aaagaaaaac cgactttttt gatagatcca cgatgataga
    47881 tgcaaaagat gtttatgacg caagtagcat tgttcttgta tggatactgt attcttttgc
    47941 ttgtgaataa gttggcgtgt cagccatttc ttcccatctt cactcacttt ttctattttt
    48001 ttcatttggt tcattttaat ttcgttacta gtgaatattt ctttaaggtc cctactagaa
    48061 aaagtccagc agctactgca aaaggaaata tgccctgata cagtctctgt aatatgatga
    48121 tacaaataaa gcttttgttt cttgagatca gctttccagc aatgcactcc tctttttcta
    48181 ttatagtagc agcaactttt ttctatcaat gagaattgcg attgtccaag aaaagaatta
    48241 ccaagtatcc accatacttg gtaactgtta ttcaaataat tttgggttct ttcaataaat
    48301 ctctggtgag acagtctaga acattggatt tctattgcta gattcgccaa caaaatatcc
    48361 ggcctttgcc gcaacctcgg caaataggct tccaaaaata ctggttctac tgactgtccc
    48421 agccaatcca tgaagcattc ctttaactgt aagtgctctt ttgattctct ttcactaaag
    48481 accgaacagt tatctgattt ttgatgggca aaatgagcac attttttctt tccctttttc
    48541 aaaatcactc tttttcgaca gccacgacaa taatatttct ttcctttttc tgcatgttta
    48601 gcaaaacagc acttattata ttcattcatt gcaagcagca tttttctccc tctgaactta
    48661 tttacgtcta agaatttatt tacacgaaaa aagagggcgt gaaaaagcta ttctgcatca
    48721 agtaataaga acagctaaac aaaaatagtt cccaatattt ttcgtttggt tgggcttgtt
    48781 accgcagatg caagcttttg aacacattta tacgaaaaaa agactgcgac acaatttttg
    48841 tcacagtctc ttactataat gctgatttat acaaaataaa atttcgttcg ctcgatagca
    48901 ttacgcgcca tcagacattg tccatattca ttcaatgttt ctggtgtacg atcgctcttt
    48961 tcagcaaact ccaaaattcg agcaatctga ttctctacat ccgcttggtt gagattttcc
    49021 atccagaagt gaacgactag gtaatagcgc tcttcgaata aatacaaatc agtccaagca
    49081 gactctagtt cgacatctgt tgcaagttgg atcacttgtt caaaatcttc aaaagcaaaa
    49141 gttgcttgtc tttcctgttc ttctagggcg cttccagata attcttcctc cggatcgctg
    49201 ccaataatct gctgacgaag aaagtcactc acatcttctg aatcaacagt agaatcttct
    49261 gattgagaaa tttcttcatc cggaagattt ttactgataa acagttctaa tccgtctcct
    49321 tttggcaaaa cttggaaagt gacagcttcg cttcctcgga actcctcttc gatatcaact
    49381 tcttccaaaa tactataaaa gaaattttcg atttctttgt gattgccaag taaatccaaa
    49441 aatgaaacgc cccgttcctc taaatcttca tgaccaatta gtacacggat cgtattttca
    49501 ttaatatgtt ccatttccat tttgctacac ctcactttgc cacaatgttt aatatagatt
    49561 cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc tcgctgttaa gcgaatttaa
    49621 taaaaccagc ggatttttca acctttcaag ctatcttatt taactataac atttttggca
    49681 gacgaatact atatagccga cttttcagct aattttccct ctctattttt cgtgcaatca
    49741 tcatcttcta ctaccttttt atacatctaa tcaagaatac tttttgtaaa aacaaaaaaa
    49801 cttggaaaaa ctatctttgt aatagt
//
LOCUS       DASNNU010000008        48046 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.008, whole genome shotgun sequence.
ACCESSION   DASNNU010000008 DASNNU010000000
VERSION     DASNNU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.008"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            271..642
                     /locus_tag="U8116_000443"
     CDS             271..642
                     /locus_tag="U8116_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN8433460.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(916..1608)
                     /locus_tag="U8116_000444"
     CDS             complement(916..1608)
                     /locus_tag="U8116_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN8433461.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1611..2396)
                     /locus_tag="U8116_000445"
     CDS             complement(1611..2396)
                     /locus_tag="U8116_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN8433462.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2633..4033
                     /gene="lacG"
                     /locus_tag="U8116_000446"
     CDS             2633..4033
                     /gene="lacG"
                     /locus_tag="U8116_000446"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEN8433463.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            4058..4375
                     /locus_tag="U8116_000447"
     CDS             4058..4375
                     /locus_tag="U8116_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN8433464.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4388..6061
                     /locus_tag="U8116_000448"
     CDS             4388..6061
                     /locus_tag="U8116_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEN8433465.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            6075..7706
                     /locus_tag="U8116_000449"
     CDS             6075..7706
                     /locus_tag="U8116_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEN8433466.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8197..9762)
                     /gene="guaA"
                     /locus_tag="U8116_000450"
     CDS             complement(8197..9762)
                     /gene="guaA"
                     /locus_tag="U8116_000450"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN8433467.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9821..10744)
                     /locus_tag="U8116_000451"
     CDS             complement(9821..10744)
                     /locus_tag="U8116_000451"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEN8433468.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10940..11545
                     /locus_tag="U8116_000452"
     CDS             10940..11545
                     /locus_tag="U8116_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN8433469.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11853..12869
                     /locus_tag="U8116_000453"
     CDS             11853..12869
                     /locus_tag="U8116_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN8433470.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13010..14311
                     /locus_tag="U8116_000454"
     CDS             13010..14311
                     /locus_tag="U8116_000454"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEN8433471.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14357..15019
                     /gene="deoC"
                     /locus_tag="U8116_000455"
     CDS             14357..15019
                     /gene="deoC"
                     /locus_tag="U8116_000455"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEN8433472.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15040..15438
                     /locus_tag="U8116_000456"
     CDS             15040..15438
                     /locus_tag="U8116_000456"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEN8433473.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15504..16586
                     /locus_tag="U8116_000457"
     CDS             15504..16586
                     /locus_tag="U8116_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN8433474.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16798..18360
                     /locus_tag="U8116_000458"
     CDS             16798..18360
                     /locus_tag="U8116_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8433475.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18353..19486
                     /locus_tag="U8116_000459"
     CDS             18353..19486
                     /locus_tag="U8116_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433476.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19486..20442
                     /locus_tag="U8116_000460"
     CDS             19486..20442
                     /locus_tag="U8116_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433477.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20541..21707
                     /gene="deoB"
                     /locus_tag="U8116_000461"
     CDS             20541..21707
                     /gene="deoB"
                     /locus_tag="U8116_000461"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEN8433478.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21731..22549
                     /locus_tag="U8116_000462"
     CDS             21731..22549
                     /locus_tag="U8116_000462"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN8433479.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22566..23273
                     /gene="deoD"
                     /locus_tag="U8116_000463"
     CDS             22566..23273
                     /gene="deoD"
                     /locus_tag="U8116_000463"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN8433480.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23571..24257)
                     /gene="gpmA"
                     /locus_tag="U8116_000464"
     CDS             complement(23571..24257)
                     /gene="gpmA"
                     /locus_tag="U8116_000464"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN8433481.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24429..25010)
                     /locus_tag="U8116_000465"
     CDS             complement(24429..25010)
                     /locus_tag="U8116_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN8433482.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25143..25826)
                     /gene="rpiA"
                     /locus_tag="U8116_000466"
     CDS             complement(25143..25826)
                     /gene="rpiA"
                     /locus_tag="U8116_000466"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEN8433483.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26145..26558
                     /gene="rpsL"
                     /locus_tag="U8116_000467"
     CDS             26145..26558
                     /gene="rpsL"
                     /locus_tag="U8116_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEN8433484.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26642..27112
                     /gene="rpsG"
                     /locus_tag="U8116_000468"
     CDS             26642..27112
                     /gene="rpsG"
                     /locus_tag="U8116_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEN8433485.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27206..29293
                     /gene="fusA"
                     /locus_tag="U8116_000469"
     CDS             27206..29293
                     /gene="fusA"
                     /locus_tag="U8116_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN8433486.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29465..30652
                     /gene="tuf"
                     /locus_tag="U8116_000470"
     CDS             29465..30652
                     /gene="tuf"
                     /locus_tag="U8116_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN8433487.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31059..31367
                     /gene="rpsJ"
                     /locus_tag="U8116_000471"
     CDS             31059..31367
                     /gene="rpsJ"
                     /locus_tag="U8116_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEN8433488.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31398..32027
                     /gene="rplC"
                     /locus_tag="U8116_000472"
     CDS             31398..32027
                     /gene="rplC"
                     /locus_tag="U8116_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEN8433489.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32055..32678
                     /gene="rplD"
                     /locus_tag="U8116_000473"
     CDS             32055..32678
                     /gene="rplD"
                     /locus_tag="U8116_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEN8433490.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32678..32968
                     /gene="rplW"
                     /locus_tag="U8116_000474"
     CDS             32678..32968
                     /gene="rplW"
                     /locus_tag="U8116_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEN8433491.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33007..33840
                     /gene="rplB"
                     /locus_tag="U8116_000475"
     CDS             33007..33840
                     /gene="rplB"
                     /locus_tag="U8116_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEN8433492.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33883..34161
                     /gene="rpsS"
                     /locus_tag="U8116_000476"
     CDS             33883..34161
                     /gene="rpsS"
                     /locus_tag="U8116_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEN8433493.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34184..34531
                     /gene="rplV"
                     /locus_tag="U8116_000477"
     CDS             34184..34531
                     /gene="rplV"
                     /locus_tag="U8116_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEN8433494.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34545..35201
                     /gene="rpsC"
                     /locus_tag="U8116_000478"
     CDS             34545..35201
                     /gene="rpsC"
                     /locus_tag="U8116_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEN8433495.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35204..35638
                     /gene="rplP"
                     /locus_tag="U8116_000479"
     CDS             35204..35638
                     /gene="rplP"
                     /locus_tag="U8116_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEN8433496.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35628..35816
                     /gene="rpmC"
                     /locus_tag="U8116_000480"
     CDS             35628..35816
                     /gene="rpmC"
                     /locus_tag="U8116_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEN8433497.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35841..36107
                     /gene="rpsQ"
                     /locus_tag="U8116_000481"
     CDS             35841..36107
                     /gene="rpsQ"
                     /locus_tag="U8116_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEN8433498.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36165..36533
                     /gene="rplN"
                     /locus_tag="U8116_000482"
     CDS             36165..36533
                     /gene="rplN"
                     /locus_tag="U8116_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEN8433499.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36570..36878
                     /gene="rplX"
                     /locus_tag="U8116_000483"
     CDS             36570..36878
                     /gene="rplX"
                     /locus_tag="U8116_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEN8433500.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36906..37445
                     /gene="rplE"
                     /locus_tag="U8116_000484"
     CDS             36906..37445
                     /gene="rplE"
                     /locus_tag="U8116_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEN8433501.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37464..37649
                     /locus_tag="U8116_000485"
     CDS             37464..37649
                     /locus_tag="U8116_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEN8433502.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37685..38083
                     /gene="rpsH"
                     /locus_tag="U8116_000486"
     CDS             37685..38083
                     /gene="rpsH"
                     /locus_tag="U8116_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEN8433503.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38115..38651
                     /gene="rplF"
                     /locus_tag="U8116_000487"
     CDS             38115..38651
                     /gene="rplF"
                     /locus_tag="U8116_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEN8433504.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38812..39168
                     /gene="rplR"
                     /locus_tag="U8116_000488"
     CDS             38812..39168
                     /gene="rplR"
                     /locus_tag="U8116_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEN8433505.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39189..39689
                     /gene="rpsE"
                     /locus_tag="U8116_000489"
     CDS             39189..39689
                     /gene="rpsE"
                     /locus_tag="U8116_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEN8433506.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39704..39883
                     /gene="rpmD"
                     /locus_tag="U8116_000490"
     CDS             39704..39883
                     /gene="rpmD"
                     /locus_tag="U8116_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEN8433507.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39927..40367
                     /gene="rplO"
                     /locus_tag="U8116_000491"
     CDS             39927..40367
                     /gene="rplO"
                     /locus_tag="U8116_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEN8433508.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40367..41662
                     /gene="secY"
                     /locus_tag="U8116_000492"
     CDS             40367..41662
                     /gene="secY"
                     /locus_tag="U8116_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEN8433509.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41724..42371
                     /locus_tag="U8116_000493"
     CDS             41724..42371
                     /locus_tag="U8116_000493"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEN8433510.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42562..42780
                     /gene="infA"
                     /locus_tag="U8116_000494"
     CDS             42562..42780
                     /gene="infA"
                     /locus_tag="U8116_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEN8433511.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42813..42929
                     /gene="rpmJ"
                     /locus_tag="U8116_000495"
     CDS             42813..42929
                     /gene="rpmJ"
                     /locus_tag="U8116_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEN8433512.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42947..43312
                     /gene="rpsM"
                     /locus_tag="U8116_000496"
     CDS             42947..43312
                     /gene="rpsM"
                     /locus_tag="U8116_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEN8433513.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43340..43729
                     /gene="rpsK"
                     /locus_tag="U8116_000497"
     CDS             43340..43729
                     /gene="rpsK"
                     /locus_tag="U8116_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEN8433514.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43809..44747
                     /locus_tag="U8116_000498"
     CDS             43809..44747
                     /locus_tag="U8116_000498"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEN8433515.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44775..45155
                     /gene="rplQ"
                     /locus_tag="U8116_000499"
     CDS             44775..45155
                     /gene="rplQ"
                     /locus_tag="U8116_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEN8433516.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45427..46266
                     /locus_tag="U8116_000500"
     CDS             45427..46266
                     /locus_tag="U8116_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN8433517.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46242..47111
                     /locus_tag="U8116_000501"
     CDS             46242..47111
                     /locus_tag="U8116_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN8433518.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47101..47898
                     /locus_tag="U8116_000502"
     CDS             47101..47898
                     /locus_tag="U8116_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN8433519.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        15194 a         8780 c        10524 g        13548 t
ORIGIN      
        1 tttagtaagt agctacacac ttctatataa attcagaagt tcttctagag tttctaaagt
       61 ttgtttctta attagcttat tgattctata atatttttga tttatatagg aaattaaaaa
      121 tattatagaa tcaacactca gagaacgggt tggtttttaa tagatttaat aagtactgat
      181 aatgaaattt ctcctggttg ggaaaacgaa gttggttacc ctgtagaaga attttttcaa
      241 gattactttg cggagtatgc cgataggata atgagagatt acgtaacaga taaaactcaa
      301 gaagtcatca atcaatccca gtcattaaat gaattatctg ataaggcgaa agaagaagca
      361 gttgccatta tttcttcagg gattagtgac tatattgata tgagtggtga aatcaattat
      421 ggcgataaag acaaaattgt acgcatgata aaagctttta tcaaaacttt tgatgtgaag
      481 gcagatctta agtttaatga tacttaccta gtaggaaaat taattaatat ccttgggaca
      541 gagaaagata cggaaacgtt tatttcctct ttgtctatgt tgtttacaga taagcatttt
      601 tcaactcagc ttggtggaga ggaactagtg gaaatctttt aaccattacg cccagcatta
      661 ataaaagtat atcaaaaaag tactcctgat gatttttaag attggtttga gaaataatgt
      721 gaaaacacta tttttaatgt taattataga tagagatgaa cgcgtcatat ggttaaaaga
      781 atgaaaaagt caaagataca ctgctatatt tcactaagga tatagcagtg tatctttcat
      841 gtcgtttcac tggcagtttt ttataaaagg aggtgtgcat ttcagttgct tttctttggt
      901 agttagttga atcgtttaag aattaacgga tgtggcagac aggtgctctg tccaaaaacc
      961 agtaaaatct ttgatggttc catctttatt tagctgtgcc tgttcaaata ctgttttgtt
     1021 tttaccgaat ggatcaattg caataattgt tccgatcccc gcacgtttgg cagcaagtaa
     1081 tcctgagtaa gcatcttcga taacaagaca atcagccgga gctaagctca gtttgtctgc
     1141 cgctttaaga aaaatatccg gttgcggttt cccagggaaa gagccgtcgt catagacaat
     1201 cttgtcccag tcaaaccagc gggctaaatc gaaaatatca aaataaaaag caacattctc
     1261 tttgacagta gccgtggcaa tcgttaatgg aatctgttgg tcagtcagct ggtcaagagt
     1321 cgcttcaagc ccttttgtca actgaagctc gttttcattt tgcaagcaaa gcatacgata
     1381 atagctttct ttttcttctg acaatacggc gatctcagag gcggtcaaat gctctgaaat
     1441 gaaatgtttc aaaatctccg tattcgtccg accatgaata ttttttaaaa tatcctcgtc
     1501 tgataaacca tcttttgtat gatcatgtat catatgcagc caagcagctt catgcagatg
     1561 agagtccaaa aacattgttc cgttgaaatc aaatataatc cctttcatag ttacgctcct
     1621 atagtggtat tttcggttga atcagcaatt tgcagatcga catattgtgc atagtgctct
     1681 tgaacctccc gcgagatctg gcgatccgta atgagcagat cgaaatcatg caaattataa
     1741 aacacataaa aatcatctcg attgaatttt ttattatcaa cgagtagata tttcttacgg
     1801 gaattattta gagcgatctg ctgtgcttct ccttcttcgg tactgtaagt agagatcgct
     1861 tcgttgtgaa cgccatttga actgataaat gcttttgtaa actttagtct tcttaaattg
     1921 tcgttggtga tcggtccgac aaatgcgccg gtatttttcc ggaaatcacc gccaatcagt
     1981 agaatttctg gtccatctgc ttgagcaaga atatcaaaaa ctggataatt atttgtaatg
     2041 atccgaattt tcttgttcag taaatgcgtc gccaatagtt cgattgttgt acctggtcct
     2101 aagaaaatcg tttctccctc gtgaatcaag gaggctgctg tggcagcaat ttcttttttt
     2161 tcttgaatct gcagagtggc tttttcagtg tgagaaagtt cttgatctaa agtgtaggaa
     2221 agactttgag cccctccatg gacacgcagc agcttgcccg atttttctaa ttcatctaaa
     2281 tctctgcgaa cggtcatatc agaaacatct aattgattga tgatttcatt aacggtaagg
     2341 attccttttt tattgaccat ttctacgata gtaagcaatc tttctttttt caacattgtc
     2401 ttcacctcta atttaaggat aatctatcat ataaacggac aacagtcaat ctaattcgaa
     2461 caaaagtaaa catttagggt ggtttttatg tttgttacaa tagattttat tgctgttttc
     2521 tggtttttat gattatttgt gtttgttttt gttgacaaat gttttggatg cgtttacaat
     2581 gcaatcagtt agaaagagcg aaaactgatt atagatttga ggagagatgt acatgcttaa
     2641 attaccagaa gattttattt ttggcggagc cacagcagcc tatcaggttg aaggagccac
     2701 taaggaaggt ggcaaaggcg ctgttgcttg ggatgacttt ttagaggaac aagggcgatt
     2761 cagtcccgat ccggcaagtg atttttatca tcaatatgca aaggacattg aattgtgtga
     2821 acgttttggt gtaaatggtc taagattatc cattgcttgg agccgcattt ttcctgatgg
     2881 agctgggaaa ccaaatccag aaggaatcgc tttttatcat cgtgtgttcg aagaatgtaa
     2941 gaaaagaaac gtaacgccgt ttgtgacgtt gcatcatttt gataccccta aacgtttgtt
     3001 tgaccatgga gattttttga atcgagaaac aatagaagca tttgtttcct atgccatttt
     3061 ttgttttcat gaattcaaag aagtcaaggt gtggagtacc tttaatgaaa tctatccagt
     3121 tgcaaccaat caatatttgt taggtgtttt cccaccagga atcaaatatg attttacaaa
     3181 gatcgtcgcc tgtcttcata atatgatggt tgctcatgcc cgtgtagtga actatttcaa
     3241 agaaaacgaa cttccgggtg aaattggtgt agttcactca ttagagacga aatatgcggc
     3301 aacagacgcg ccggaagata agcatgctgc ctttttagac gatgcattgt cgatccgttt
     3361 cttattagat gctacctatt taggctatta ttcaactgaa accttgactg ctcttgatga
     3421 gatatgtgaa gcaaatcagg ccagctatca ttttcctgaa gaagatttcg ttgaattgaa
     3481 aaaagcatcc acgcgtaatg actacttagg gatcaatcac taccaatgcc attttgtcaa
     3541 agcttacgat ggagaaaatg caatccatca taacggtacg ggtgaaaaag ggacctctgt
     3601 ttataaagtg aagggaatcg gagaacgtat ttataaagaa ggaattcctc gaactgattg
     3661 ggactggctg atttatccag aaggtctata tgacttgttg ctgcgaatta aaagcgatta
     3721 cccacattac aacaaaatct atatcactga gaacgggatg gggtataaag atcagtttga
     3781 agatggcatt attatggatc agccgagaat cgactatttg agagtctatt tggaatcctt
     3841 aagcaaggcg ataacagccg gggtaaatgt gaaggggtat tttttgtggt cgcttatgga
     3901 tcttttctca tggacaaatg ggtataacaa aaggtatggc ttgttttatg ttgattttga
     3961 aacccagaag cgttacccaa aagaatcagc ttactggtac aagttggtca gtgaaaccaa
     4021 aacgattatt taataagtga taatggagga agaaaagatg aataaagagg aattacaaat
     4081 gcttggtttt gagatcgttg catattcagg cgacgctcgc agtacattat tgaaattatt
     4141 gaaagaagta agacaaggaa actttgaaca tgttgattcg gcgttgaaag aagccgacga
     4201 aaatttaacc cttgcacata attcgcaaac aaaaatttta gcggaagaag catctgggaa
     4261 agagatggag atgggattca tttttatcca tgggcaggat catttaatga ccactttatt
     4321 gctgagagat ctgattcaag actttattgt actgtacaga cagaatcaag gataggagaa
     4381 gaacgctatg gatggaatca ttaaacaaat cgaaaaaggg aaaccatttt ttgaaaaaat
     4441 ctcacgtaat agttatttag gagccgttcg tgacggattt ttaacagcga tgccagctat
     4501 tttattttca agcattttca ttttgattgc tgcggttcct gagatttttg gttaccaatg
     4561 gcctgaatct gtctccacat ggctatggaa agtctataat tattcgatgg gtgttgtagg
     4621 gttactcgtt tcagcaacca ctgctcgctg cttggctgat tcgatgaatc gtcgcttacc
     4681 gaaaaacaaa gtaatcaaca ccacctctgt gatgctggcc tctgtggtaa gtttcctgtt
     4741 gttaagcgtc tctccgattg aaggtggatt ttcaacggaa tatatgggga caaagggact
     4801 actggcttcc tttgtcgctg catttttaac agttaattta tataagtttt gtgtgctgaa
     4861 agatattacc attaaaatgc ctaaagaagt tcctggaacg atttcgcaaa ccttccgaga
     4921 tatttttcct ttctcttttt cagtatttgc ggccgtttta attgatttag gcattcgaca
     4981 aatcttccaa atctcatttg cagaagcaat cattacggtg ctgcagccgc tatttacagc
     5041 agctgacggg tacttaggaa tcgctattat ctggggagcg atggcgctgt tttggtttgt
     5101 cggtgttcat ggaccgtcga tcgtggagcc ggcaattgcg gcaattatct atgccaatgt
     5161 ggaaaccaat ctgcaattgt tcaattccgg tgaacaagct tctaaagtac tgacggtggg
     5221 tttagggaat tttgtaggaa ctatgggagg aactggggca acattggttg ttccttttat
     5281 cttcctactc tttgctaagt ctaagcagtt gaaggcagtt gggaaagctt cctttattcc
     5341 agtaagtttc gctgtcaacg aaccattgct atttgcggca cccatgattt tgaaccctta
     5401 tttttttatt ccatttttgt taacacctgt tgtaaacgtt tgcctgttca agttttttgt
     5461 cgatgtattg ggcatgaaca gctttatgta cgttttgcct tgggcaactc cagcaccgat
     5521 cggtttggtt ctaggaacgg gcatgggtat cttagctttt gttctagcag cggttctaat
     5581 cgtcgttgac ttcctgatct atctaccatt ttgcaaagca tacgatgctg tcttagtaga
     5641 gaaagaagca caaaaagcag aggtgatcgc aaccgaggaa ggtgcttctg ccgatgtacg
     5701 tgaggaagta cctgtggcag cgagtcaagt tatcagcgat gcaaataaag aaatcaatgt
     5761 gttggtactt tgtgctggcg cagggacaag tgggatgtta gctaatgctt taaaaaaagg
     5821 tgcatctgaa tcgaaagcac cgatcagtgc aatggcgggt gcttatggtt ctcattacga
     5881 aatgctgcag gattttgata tggtggtctt agcaccgcaa gtcaattctt actatgatga
     5941 tatcaaagaa gacaccgatc gcttaggaat caagctggtt gcgacaaaag gagcagaata
     6001 tatcggatta acgagagatc ctgaaaaagc tgttcagttt attctgaacc aatttcaata
     6061 aaggaggagc cataatgaaa atgggaattt ggttgcgtaa agctgtttta tttgcagctg
     6121 tgttaggggc tttgggggct gtttcaaaaa ctgttgaagc atctgccagc gaatttattc
     6181 gcggagcaga tatttcgatc ctgtcagata tggaaaaatc tggtgcaaaa tattatgaag
     6241 acgatatttc aaaagatgcc ttagagattt taaaaaataa tggtgtcaac tatgttcgac
     6301 tgcgtttgtg gcaggatcct tataatagta atggagaaag ttatggagca gggacaaatg
     6361 atctggaaac gacgattgac cttgcaaaac gagcaaaaaa tctgggactt aaggtgctgc
     6421 tggattttca ctacagtgat ttttgggttg atcctgggaa acagaatctt cctaaagcat
     6481 ggcaaggtct gacctttgaa gaaatgaata cggcattgta cgactataca aagaacgttc
     6541 tgtcggaaat gaagcagctg gacgtttatc ctgatatggt tcagatcggc aatgaactga
     6601 atagcggaat gctctggcct tatggcaagt cttggggaga aggcggggga gaatttgatc
     6661 gattggctgc ttttttgaag tctggtatcc aagctgttcg agacactcaa cctaaaacaa
     6721 ctccagtaat gctccactta gctgatggtg gcgatacagg agcatttact tggtggtttg
     6781 atgaaatcac gagtcgaggt gtgtcatttg atttaattgg agtgtcgtat tatccgtatt
     6841 ggcacggttc aatgaatgaa cttcaaacca atatgaatgc gatcagccaa cggtatggta
     6901 aagaagtgat cgtagttgaa acagcttatg ggcatacaac agcgaatgcc gatactatgc
     6961 caaatgcttt tggtgaagcc gaagcagctg cggggggata tgaaccaaca ccgacaggac
     7021 aagcagagta tttgctggat cttgccgatc ggattcaggc agtaccgaat aatcgcgggg
     7081 cgggcttctt ctattgggaa cctctttggt acaacggaaa tgtgagttgg gcaacacaag
     7141 caggtatgaa ttatctcagt gttcaatcca ctatgggaaa cgaatgggac aaccaagcga
     7201 tgtttgattt tgctggaaac gccttgccaa gcttgcgtgc gtttaaacaa gcaggggcgc
     7261 aaataaatct agttaaaaat gcaagtttcg aaagcgatgg accaacgacc agtccttcca
     7321 attggcaaac ctggtttcaa caaggaaccg ctaagggtac gatcaaaacg gaagcgggca
     7381 ctttttttgg ggactacaaa ctaacttttt ggcaggatca agcatacgag gcatcagctt
     7441 atcaaaatgt tagtggcata agcaaaggaa cttacacatt gagtgcctat gtaatgtctg
     7501 ggggagagtt tgatactaat cagttatata tcaaaaatta tggtggagcg gaactaaata
     7561 ctgcggttgt taaaacaagt gaatggacaa aaattacaat caaagacatt ccaattacaa
     7621 caggtgtttg tgaaattggg gtttatagca aggcttcagg aaatgcttgg acgaacgttg
     7681 attttgtttc attggtgaaa caatagactg cggatcgttt cgattcaaga tcggaggttc
     7741 ccttcagcca catcgttagc gtagaaggca ggaatcgaac ctgcacctcg cctttgccaa
     7801 aaataaccag aaacgatttc cagtcatttt taccaaaacg agtaaagcac cagacttcat
     7861 ctacactttg tattatgcta aagagagttc aacgatttcg taagaagcaa tgaaaaaatt
     7921 gcaaaaagtt gtgcagataa cataaagttt gtttaagaaa gggtaacctt agaattccgt
     7981 cgtgaatatg atttgagcac ttagatggat agaaaaaagt tctcggaaaa cagttcgttt
     8041 ttcgagaact tctttttatg ataagaatga aacaaattaa cttatattat agtctctttt
     8101 ggtagggaac catcttattt ttaatgaaaa tcaagcattt tagctattga agaatgttga
     8161 tttataggga ttccccctcc atattatgtt ggctctttat tcccactcca ctgttgctgg
     8221 tggtttagaa gtgatgtcat acacgatacg gttgacgtgg gctacttcgt ttacgatacg
     8281 tacagagatt ttttgcaaga cttcccatgg gatacgggca aagtcagcgg tcattccgtc
     8341 gattgaagtt acagcacgga taccgactgt gtagtcgtaa gtacgtccgt cacccataac
     8401 accgacagag cggatccctg gaagaacagt gaaatattgc cagatatcac ggtcaagtcc
     8461 agcagctgcg atttcttcac gcaagatagc atcagattcg cgtacgattt ctagcttgtc
     8521 ttcagtgatt tcgccaagaa cacgaatacc aagaccagga ccagggaatg gctgacgcca
     8581 aacgattgaa tcaggcatgc ctaactgagt ccctaattca cggacttcgt ctttgaataa
     8641 tgtgtttaat ggttcgatca attcaaattg catatcttct ggcagtccac ctacattatg
     8701 gtgagatttg atcgtctgtg ctgtttctgt accagattcg ataacgtcag tatacaacgt
     8761 tccttgtgct aggaaagaga cgccttcttc accagcaagt tttgttgctt cgtcatcaaa
     8821 aacatatacg aattcattcc caatgatttt acgtttttgt tctgggtcag atacaccttt
     8881 caacttgtct aagaagcgat ctcttgcatt gactttgatg atgttcaagc cgaattttcc
     8941 gcctagactt tccattactt gttccgcttc gcctttgcgt agcagaccgt ggtcaacaaa
     9001 gatagatgtc aattggtcac cgatcgcttt ttgtaaaaga accccaacga cactagaatc
     9061 aacgccacct gaaagaccaa gcaagacttt tttgtcacca acttgttcac ggattttttc
     9121 tacttgcata tcgataaagt tatccattgt ccagttacct tcacagtgac aaacatcaaa
     9181 ggcaaagtgg cgcagcaatt cgattccgta ttcggaatga cgtacttctg catggaattg
     9241 gataccataa aaattacgtg ttgtgtcttg gatagaagca atcggacaat ctttgcttgt
     9301 tccgactgtt tcaaagcctt caggaacttt agttaccaag tcaccatggc tcatccatac
     9361 tgtttgttgt tttggtgttt ctttaaacaa gacagcttga tcgcttgtta cttctaacaa
     9421 ggctttccca tattcgcggt ttttagcagg ctctacggaa ccgccaagtt tgtacgtgat
     9481 caattgcata ccatagcaaa tccctaatac aggaatacct aaattgaaga tctcaggatc
     9541 gatcccaaat gcattctcat catatacact gtttggtccg ccagaaagaa tgattccctt
     9601 tggattcatc gcttttactt cttctgcagt aatgcggtgg ctcaataatt cggaaaagac
     9661 tccaagatca cgaatacgtc ttgtgatcaa ttggttatac tgactaccgt agtctaatac
     9721 gataatcttt tcaacgttcg tcatatcggc aacgtttgtc aaatttatta cccctatccc
     9781 tttaaatttt atctcaaaat agtcaaacta ttttaaaatg ctaatatttt cgcatgaaaa
     9841 tcgaatcgat gatatgattt tcagttttat ggagaattat atcggctctt cctcgtgtag
     9901 gcaagatata ttcctgtaaa tttttcaaat tcacattttt ccatacatct tttgccattt
     9961 taaaggcttc ttcacgatct ccgatagcaa aacgataata ataattctct ggattttgga
    10021 aagccgtatc cagtaacgca ccaaaacgat ccaaatacca tttttcgatc agcacgggat
    10081 cggcatcgac atagacagaa aaatcaaaaa agtcgctgac gtagatttgc tgattcgcag
    10141 gtaattgaag cgtattgatc ccctcaacga tcaagatatc aggctgctgg atcaattcgt
    10201 acttgtctgg aatgatatca tacacatcat gtgagtaaac aggtactttt atctcttcct
    10261 gaccagattt tacacggttc aagaaatcga tcagcatctc catatcataa ctttcaggaa
    10321 atcctttacg gtccatgatt ccacgctctt ttaatatacg atttggataa agaaatccgt
    10381 cagtcgtgat cagctgaacg tttcttcgtt tgaaggtacg agacaaaatc gtctgcagca
    10441 gtctggctgt tgtacttttt ccaacggcta cacttccggc gatcccgata ataaaaggcg
    10501 gtacttctac gtatttatga agaaataaac ctttactcaa gaacaaagat tcaaattctt
    10561 tcatataaag ataaatcaga tgagcaagag gtacatagat atctcgaaca tcctgcatgg
    10621 aaatgcggtc attcaaactt ttgatattat ctagttcatc ttgtgtcaaa ggtgcttgcc
    10681 cattacgata aaattctcgc cattcttctc ttgccacacg gtagtagttc attggatcat
    10741 tcatattagc ctcctgcgtt ctcccatcaa tggaactatt ttaacacaaa ataaacgcaa
    10801 accccaccac ttaagagaga ttgccgatct ttttgtataa tctttataat agaaagaaac
    10861 ggagtcttat tcttgacttg cgcacggata cataaaagga tagactgttg tcatgaaaga
    10921 aatgagaagg atggaagaca tgacaaatca ttactattct gaacgcccag atacggcgca
    10981 cgattttgaa caatgggctt ttgagttaaa aggcagaacc tttcaatttg taacagattc
    11041 tggtgtattt tcacgtgaaa cagtggatta tggttcacgt gtcctgattg atgctttcga
    11101 ttgggaagag ttacctgatg aaggaaagat cttagatgtg ggatgcgggt atggaccgat
    11161 tgggttagct ttggcatttg cgacacagcg gtttgttgaa atggtcgaca ttaatgaacg
    11221 agcggtaagt ctagcgcaag gaaacgcaaa gcgtaaccaa atcgaacatg ttgatatcca
    11281 tcaatcgaat atctatgaag cagttcatga agaaacctat gcagctatcg taagcaatcc
    11341 gccgatccgt gctggaaaaa aagttgtaca tgaaattttg acaggcgcat atcctcggtt
    11401 gaagaagggc ggtacactga caattgtgat ccagaagaaa caaggagcgc cgagtgcgca
    11461 aaagaaaatg gaagaaacat ttggtaatgc agaaatcgtc acaaaagata aaggatatta
    11521 tatcatcaaa agtgtgaaag aataactagc tgaaaaatag tggagcgacg aaagagtgga
    11581 actgggcgtg catgtacggg tacgtatcag ttcttttttt gttgagaagt ttttaaaaac
    11641 aaagaactat tttttcttca aaaagaaata gtaggaacag agtcgttgtt ttttacgata
    11701 aaataaataa gatacagaac ttgtatagaa ggctgccaag tcggtagata aaggaggttt
    11761 ttatcaaaaa acatactttc cttttttctc tattcttttc ttgggggttt tggtacactg
    11821 atagggaatt tagatagaag ggagccaata ggatgaagct gaccatcaga gatatcgcag
    11881 aaatggcagg cgtatcagta acaactgtct cacagatttt gaataacaaa ggaagcagat
    11941 ttagtgataa gacaagaaaa aaagtgttgt cgatcgtgaa tgaacatcat tataagcccg
    12001 attatttcgc ttcaaatgtg atcaatcgac attcaaagac catcggaatg atcgtccctg
    12061 atgtcactga tttcttcttc tccaaactga tcgaaggtgt agagagctat ttgaatcctt
    12121 tgggctatgt gatcatgtta tgcaattctc gtcatagcca agaaaatgag atcaagtatt
    12181 tacaagagct ttcccaccgc tcagtggacg gaatcttgct ggccacacca aatattttac
    12241 ctgagaaata tgcattagac agcggttttt atcaaaaaat gccaatcatt ttaatcgatc
    12301 gcggattgaa tcgtcgggat aacggacggc ttatcgtaaa ggaatatgag ggcgcctatc
    12361 aagctgtttc gctgtttatt gaaaatggcc atacgaagat cggcatgctg aaggaaacca
    12421 ctggatacta tcaattagaa gaacgtttca acggatatcg tcatgcgctg aaagatcatg
    12481 gcctgccttt taatgggaaa tttgttgaac atggagagtt gactgtccaa ggtggatata
    12541 ctgcatcaaa aaaattactc aaacataaag atatcacggc tattttttgc gggaatgatg
    12601 ccatggcgat cggatgctat caggcgatcg atgaattggg caaaaaaatc ccagaagata
    12661 tctcagtcat aggattcgat ggattaaagc tgtcagaata catgattccg caattgacga
    12721 cagtgcagca gccaagtttt gatattggat tttatgctgc acgtttcctg atcgatacga
    12781 tcgaatttcc tcaaagaaaa gttccgaaca aagtgttcga aacaaaatta attattcgtg
    12841 agagcgtaaa agtattgaca aagggctaac tcggcggtat attgcttatg taagcattta
    12901 caaaggctag ttagtgcttt ttttattttt agtaaaccag tttactaaag tggtttacct
    12961 ttcctaaaaa taatgtataa taccgaatga agacaaggag gatcaggcaa tgcgaatggt
    13021 ggatctgatt gaaaaaaaga gggatggaaa cgagctgtcg aaagaggaaa tcgagtacat
    13081 cgtcacgaac tatacgaatg gaaagattcc agattatcaa gtcagtgcat tattgatggc
    13141 aattttttat caagacatga caaatgaaga aatcacgaac ttaacattgg cgattgctaa
    13201 ttctggtgat gtcatcgatt tgagttctct tgaaggaata aaggtagata agcattcgac
    13261 aggtggcgtt ggtgacacga ccacattgat tttagccccc ctagtagcta gtgtcggagt
    13321 gacagtggct aaaatgtctg gacgcggtct aggttacaca ggtggtacac tagacaagct
    13381 agaagcaatt cccggatttc agatcgagct ttcggatgaa gaatttgtac gcattgttaa
    13441 tgaaagtaaa gtagcggtaa ttggtcaatc aggaaacttg gcaccggcgg acaaaaaatt
    13501 gtatgccttg cgtgacgtca cagcgaccgt tgattctctt ccgctgattg ccagttcgat
    13561 tatgagtaaa aaaatcgcgg caggagcaga tgcaatcgtt ttggatgtga ccactggaga
    13621 cggtgctttc atgaagaaca tcgaagatgc aagacgtctg gcaaagacca tgacgagcat
    13681 tgggaaactg gctaaccgag aaacagtggc ggttatctca gatatgtcag aacctcttgg
    13741 cgaagctatc ggaaatagtc tagaagttgt cgaggcaatc gaaacattac aaggaaatgg
    13801 tccagaagat ctagtagaaa tgtgctacgc cttaggaagc caaatggttg ttcttgctgg
    13861 caaagctaag acaaccgatg aagcaagaac gttgcttcaa gaagctctgg aatcaggtaa
    13921 agcgttagca aaattcaaag aaatgatcca aaatcaaggg ggagacccag cgatcgtgga
    13981 acatccagaa cgaattttga ctgcacgcta cacaatggag cttcctgcta aacaaagcgg
    14041 agttgtgtca aaaatcgtgg caaatgaatt aggaattgct gcaatgatgc taggtgctgg
    14101 gcgtaaaacc aaagaagaag atattgatca cgctgttgga ttgaaactgc ataaaaaaat
    14161 tggtgacact gtgacaaaag gtgagtcact attgacgatc tatagtaacg acgaagaaat
    14221 cacttctgtc attgaattat tatataaaaa tattgaaatt ggtgaatcgg caatgaagcc
    14281 aacgttgatt catgacatca tcacagaata aaggaaaaaa gcggacttta ccgttttaaa
    14341 gaaaagggga aaagaaatgg aactaaaccg aatgatcgac catactattt tgaaagcaga
    14401 cgcatctaaa gaagctgtga tgcaaattat cgaagaagca aaaaaatacc atttttattc
    14461 tgtctgcatc aatccagcat gggttgcgct agcgaaagaa caattacaag gtgagccagt
    14521 agccgtatgt acagtaatcg ggtttcctct aggagcaaac acacccgaga caaaagcatt
    14581 tgaaacaaca gatgccatca ataacggtgc agatgaagtc gatatggtca tcaatatcgg
    14641 tgctttgaaa tcaaaggatg atcaacaagt ccaaaaagat atcgaagcag ttgttgaagc
    14701 agcaaaagat cgtgcgcttg tcaaagttat tattgaaaca tcgttattag atagagaaga
    14761 aatcatccga gcttgcgaaa tagcaaaagc tgcaggcgct gattttgtca aaacatcaac
    14821 tggtttctca acaggcggag caaaggtaga ggatgtccgt ttgatgcgtg aaactgtagg
    14881 accagaaatg ggtgtcaaag cttccggcgg tatccataac gaagaagaag caatggcaat
    14941 gatcgaagct ggtgctacaa gaatcggaac aagcgcagga gtagcaatcg tatctggttc
    15001 aactggtgaa ggctattaat tttttcaaag gagaagctca tggcagaact caaacaagaa
    15061 tggatcaaca cagctattga agcgttagat aaagcctatg ttccttattc ccattttccg
    15121 gtaggcgctt gtctcgtaac ggaatcaggc aagatttatc aaggcatcaa cattgagaat
    15181 gcatcatttg gattaacgaa ttgtgccgag cgcacagctt ttttcaaagc agtatctgaa
    15241 ggagaaaaaa catttacgca tctagtcgta gcggggcaca cccccgatcc tatctctcct
    15301 tgtggtgcat gtcgacaagt aatggcagaa ttctgttccc ctgatatgcc ggttacgcta
    15361 gtaggagaca atggtgtgac gaaggcaacg accgttcgtg cactgctgcc ttatgcattt
    15421 actgaaaaag atctataact catgttcatt cagtcagctg actgttgaaa aataaaacaa
    15481 ttattttagg aggctttaca gaaatgaaaa aagcaaaatt atttggttta ggtgctgtcg
    15541 cactagcagc agggttattt ttaggtgcat gcggcaacaa cggctctact gattcatccg
    15601 gtggcaaaag ctcatcagac acaacaactg cagcattgat cacagacaca ggtggtgtag
    15661 acgaccgttc attcaaccag tcagcttggg aaggactaga aaaatggggg aaagatcaag
    15721 gactttcacg aggaaatgat ggcttccaat atttccaatc ttcaaatgaa tcagattaca
    15781 tcccaaatat tgaccaagca ttgaatgctg gattcaaaac gatttttggt attggttata
    15841 aactaaaacc agcgatccaa gaacaagcaa caaacaatac aggtacgaac tttgttatta
    15901 tcgatgatgt catcgatgga ttagataacg ttgtatctgc aactttcaaa gacaacgaag
    15961 cttcttatct tgcaggtgtt gcagcagctt atacgactga aacaaacgta gtcggcttta
    16021 tcggcggtgt aaaaggtgaa gtaatcgatc gttttgatgc tggattcaaa gccggtgttg
    16081 atgcaggtgc gaaagaatta ggaaaagaaa tcaaagtatt gaatcaatat gctggtgact
    16141 tctctgctcc agataaagga cgttcaattg ctcaaggtat gtatgcacaa aacgcagata
    16201 tcatcttcca tgcttcaggc ggtacaggta acggtgtatt ccaagaagca aaatcattga
    16261 acgaatcagg cgacaaaaaa gtttgggtta tcggtgttga ccgcgaccaa tcagacgaag
    16321 gcgaatacac attaaacggc gaaaagaaaa acttcacatt gacatctaca ctgaaagcag
    16381 ttggaacagt agtagaagat ttggcacaaa aatcagcgga cggtaaattc cctggcggcg
    16441 aacacactgt ttatggattg aaagaagacg gtgttggtct aacagaagga caactttctg
    16501 acgaagcgaa aaaagcagtt gatgaagcaa aagaaaaaat catttcaggt gacgtaaaag
    16561 ttccagaaac tcctgaagaa aactaaaacc atttctatag ccttgttaga cacatgtttg
    16621 acaaggctat ggtttgatta gaacaagaat tgttcattta tccaagctgc cttgtgacag
    16681 tttggataag ggaacaactc cctgaagtaa tgtttgtttc aaaagaagaa cgctcactaa
    16741 gcgtgaatct tagtcaatcg ttgccctaga tagaaatcgt taggatgtga aaaaaaggtg
    16801 gttcaagaaa atattgtcat agaaatgcag aatattacca aagaatttgg aacattcaag
    16861 gcaaatgaca acatcaattt acaagtaaag gcaggagaaa ttcatgcgtt gcttggagaa
    16921 aacggtgctg gcaaatctac attgatgaac gtgctttccg gattattaga gccgacatca
    16981 gggaaaattt tgatgcgtgg gaaagaagta aagatcacaa gtccgaccaa agccaaccaa
    17041 ttagggattg ggatggtcca tcagcacttt atgcttgttg atgcctttac tgtaacagaa
    17101 aacatcgtgt tgggaagcga gcctagtcgc gcagggatgc ttgaccataa aaaagcacga
    17161 aaagagatcc aaaaagtttc tgaacagtat ggattgtcag tcaacccaga tgcttacgtt
    17221 cgtgatattt cagttggaat ggaacaacgg gtagaaattt taaaaacact ttaccgagga
    17281 gcagatgtac tgatttttga tgagccgaca gctgtattga cccctcaaga aattgatgaa
    17341 ttaatcgtga tcatgaagga attagtcaaa gaaggcaagt cgatcatttt gattacgcat
    17401 aagttagatg aaatcaaagc agtagctgac cgctgtacag ttatccgccg tggaaaagga
    17461 atcggtacag tcaacgttaa agacgtaacc tcacaacaat tagccgatat gatggttgga
    17521 agaacggttt cattcaaaac gatgaaaaaa gaagcgaagc ctcaagaagt cgttttgtct
    17581 attgaaaatc tagtggtgaa agaaaatcgt ggattagaag ccgtgaaaaa cttgaactta
    17641 gaggttcgtg ctggcgaagt acttggtatc gctggaatcg atggaaacgg gcagtcggag
    17701 ttgatccaag ctttgactgg tttgcgaaag gcagaaagcg gacatatcaa gctaaaaggg
    17761 gaagacatca ctaataaaaa accccgaaaa atcactgaac atggtgtagg acacgtgcca
    17821 gaagaccgtc ataaatacgg gttagtccta gatatgacat tatctgaaaa tatcgcacta
    17881 caaacctatc atcaaaaacc ttacagtaaa aacggcatgc tgaattattc agtaatgaat
    17941 gaacatgcca gagaattgat cgaagaatat gatgttcgaa caacgaatga acttgttccc
    18001 gcaaaagctt tatcaggcgg aaatcagcaa aaagcaatta tcgctcgtga agtcgaccga
    18061 gatcctgatc tgttgattgt tgcaaatcca actcgtggac tggatgtagg tgcgatcgaa
    18121 tttattcata aacgtctgat cgaacaaagg gacaaataca aagcagtgtt actgatcagt
    18181 ttcgaattag aagaaatttt aaatgtttcg gatcgtattg ctgttatcca tgaaggagaa
    18241 attgtcggta tcgttgatcc gaaagaaaca tctgaaaatg aattaggtct attgatggcg
    18301 ggctatacct tagaagaagc acgaaaagag ttagaaaagg gggctggcgc acatgaatga
    18361 tcgcagcgaa aaattccgta atttattagt gcctgtactt tcagttgttt taggattgat
    18421 cttaggcgcg atattgatgg ctgcttttgg ttttcagccc atccaaggtt attcttctat
    18481 gctagatgca gcccttggtt cgcaaagagg gataggtgaa acgttaaggc aagcaacgcc
    18541 gttgattttt acagcattag gtttttctgt agctaactca gctgggtttt tcaatattgg
    18601 gctttctgga caagcacttt gcgggtggat cgtcagtgtc tgggtggcac ttgcgtttcc
    18661 agatctgcca aaagttgttt tgcttccgtt atgcgtgatc ttaggtgcct tagctggtgc
    18721 gatggcagcg gctgttccag gtttacttcg agcatttttt ggaacaagtg aagtgatcgt
    18781 aacaatcatg atgaactata tcttattata cgtaagcaca tttgctttac atgaagtgat
    18841 gcctgaaagc tatcgctcaa gtttggactc ttccaatatg atcagtcaaa atgcctcact
    18901 gcggttacca tggttaacac aagcttttgg cggctctcgc gtaaactcag gattattttt
    18961 agcaatcatc gcgcttattt tcgtttggct gctgatgaag aaaacgactt taggatttga
    19021 aatccgttct gttggattga atccttttgc ttctgagtat gcaggaatga gtagtaaacg
    19081 tacgatcgtt ttgtcgatgg tcatttctgg cgctttggca gggcttggcg gtgtggttga
    19141 aggattagga acgtatcaaa acttctttgt tcaaacgact tcgctatcta ttggatttga
    19201 cggaatggct gtttccttgc ttggttctgg atcagcaatc ggtattcttt tatcagcgat
    19261 cttgttcagt attttgaaga tcggtggact tggcatgcag acaggagcag gtgttccgtt
    19321 tgagatcgtc aatgtatcga ttgctttgat tatcttcttt gtcgggatca acttcctgat
    19381 tcgtttcgtc atggctaaag tcttccaagg gaaaaaacaa gaagagatcg ttgcgacaat
    19441 agaatcgaaa ccaacgaatc aaaaccatga agggggcgaa ctataatgtc gactatcgaa
    19501 ttaattgcct tgatccttac ttctacactg gtttattcaa caccgctaat tctaacgtca
    19561 ctaggcggaa ctttttctga acgaagcggg atcgtaaacg tagggctaga gggaatcatg
    19621 gtcatgggtg cttttagttc catcgttttc aacttgacga tgagtccatc acttggttct
    19681 atgacaccat ggatcgctat tttagtgggt ggcgtcgtag gtatcctatt ttctctgatt
    19741 cacgcagtag caacagtcag tttgcgtgct gaccatatta tcagcgggac agtaattaat
    19801 ctgatggcac ctgctttagg tgttttcttg atcaaagcat ggtatggtaa aggtcaaact
    19861 gataacatca cgcagaatct ggggtatttt tctttcccag gattagcaga tattccggtc
    19921 attggaccaa ttttctttaa aaatacattc ttacctgctt attgtgctat tttgattgct
    19981 gtcctagctt gggtgatcat cttcaagacg aaattcggct tgcgcctccg ttcagttggt
    20041 gaaaatcctc aagctgccga cacattaggt atcaatgtat atgctatgcg ttattcaggc
    20101 gtactgattt ctggcttgtt aggcggtatg ggcggtgctg tttttgctca gtcgatttcc
    20161 ggtaactttt cagctggtac gatcgttgga caagggttca tctccatggc tgctatgatc
    20221 tttggtaagt ggaatccttt aggggcaatg ggtgcctcat tgttctttgg ttttgctcaa
    20281 agcttgagta tcattggcgc acaattacca gttatttctt cgattccgcc agtattgctg
    20341 caaattgctc catatttgct gacaatcatt gtgctggttg ttttcttagg gaaagcttct
    20401 ggtccaaaag caaacgggaa gaactatatc aaatcaaaat aatcttttag cgaataaaaa
    20461 gggtgggtta cgggaagtgt ttttcgtctc gccctttttg ttttaaaggc aataaaaata
    20521 aaaagaaaga aggaataatc atgtttaaac gcgtacatct gatcgtcatg gattcagtag
    20581 gaattggaga agcaccagat gccgataagt tcggtgatgt tggttctgat acattaggac
    20641 acattgcgaa agaagcagga ttgaacgttc cacatttaga gcagcttggt ttaggaacga
    20701 tccgtcctct tgaaggtgtg gaatcagtga aagatcatca aggctatgcg acgaaattag
    20761 aagaagtttc tgtagggaaa gatacgatga caggccactg ggaaatcatg ggattgaata
    20821 tcaaaactcc atttcgtgtg tttcctaatg gattccctga tgatctgatc aaacaaatcg
    20881 aagaattttc tggccgtaaa gtcgtatgta acaaacctta tagcggaaca gctgttattg
    20941 acgattacgg tgaacaccaa atgaaaacag gcgatttaat tgtatatact tctgccgatc
    21001 ctgtccttca gatcgcggca catgaagaaa tcattccttt agaagaatta tatcggattt
    21061 gccaattcgt tcgtgatata acaaaagatg atccgtatat gattggccga atcattgcac
    21121 gtccgtacgt gggtgaacca ggagacttca cacgaacaag caatcgccat gattatgcgt
    21181 tgaatccatt cggacacaca gtccttgatt ctttgaaaga agcaggaaag gacgtgattg
    21241 ctgttggaaa gatcaacgat attttcaacg gacaaggaat cactgaatct gtccgcacga
    21301 aaagcaatat ggatggcgtt gaccagctgc tgaaagttat gaaaaacgat ttttcaggaa
    21361 tcagctttac caatttagta gattttgatg cattatacgg tcatcgtcgg gatgtagtcg
    21421 gctatgcgca tgcgattgaa gaatttgact tacgaattcc agaattgctt gaagcgatgg
    21481 aagaagacga cttgctaatg attacagccg atcacggaaa cgatccgacg tttactggta
    21541 ccgatcatac gcgagaatat gtaccgttat tggtttacag taaaaaaatg aaagaacaag
    21601 gggcattgcc tcaaggatat tattcagata tttcagcaac gattgctgag aactttagtg
    21661 tttcttcgac tgaaaatggc gaaagcttct tgaaattatt aaaataatct ttttaaatag
    21721 gagaaagcaa atgaccaagt taagcgagat gttacaagaa acgacccagt ttatccttga
    21781 taaaggagta aaagaaattg attttggttt gattctaggt tcaggattag gtgaattagc
    21841 agatgaaatc gaagatgctg taatgatttc ttatgatgag atcccgttct ttcctgtttc
    21901 gacagtagtt gggcatgcag gaaagctagt ttatgggtct ctggcaggca agaaagtgtt
    21961 agccatgcaa gggagattcc actactatga aggtcattcc atgcaaacag tgacctatcc
    22021 agtacgtgtg atggccggac taggggccca ttcagtcatt gtgactaatg catgtggtgg
    22081 tgtcaacgaa tcatttgtac caggagattt gatgctgatt accgatcata tcaattttac
    22141 tggtgaaaat cctttgatcg gtccgaatga agatgcaatg ggccctagat ttccagatat
    22201 gagtcaagct tatacgcctg actatcaagc aacagcaaaa gcagcagcag aaaaactagg
    22261 tttggatctt aaagaaggcg tatatatggg ctattctgga ccaacttatg aaacgcctgc
    22321 ggagatccga atgtctcgga caataggagc cgatgcagta gggatgtcga ctgttccaga
    22381 agtcatcgta gcagctcaca gcggattaaa agtgttagga atctcttgta ttaccaactt
    22441 agcagcaggt atgcaagcga atctgagcca tgaagaagtt gtagaaacaa ctcagagagt
    22501 caaacaatct tttaaagcat tggttaaaga aacattgaca ttattgtaat aggaggaaca
    22561 ataaaatgag tgtgcatatt gaagcgaaag aaggcgagat cgccgataag attttacttc
    22621 cgggagatcc tttgcgagct aaatatattg cggagacttt tttggaagat ccagtttgtt
    22681 acaatcaggt acgcggtatg ttaggcttta ccggcaaata taaaggagaa cgtatttctg
    22741 ttcaaggaac aggaatggga atgccatcag ccaccatcta tgcccatgaa ttgatccagt
    22801 cgtatggtgt aaagaaattg attcgagtag gtacatgcgg tgcattgtca aaagacgtcc
    22861 atgttcgtga tttagtacta gcacaaggtg cagcgaccag ttcttcgatg atcgaaaaaa
    22921 atttccaagc ctttcatttt cctccaatca gtgatttcaa tctattactg aaagcatatg
    22981 agatagctaa agaaaagggc tatacggttc atgtaggaaa tgttctttcg gaagattctt
    23041 tctacaaaga tgatttgaca gagacgttcc aattagctga acttggcgtt ttaggagtgg
    23101 aaatggaagc agcggctctt tattacttag gcgcgaaata ccatgtgcag acattaagtt
    23161 tgatgaccgt tagcgaccat ttgatcaccg gtgaagaaac aacagctgca gaacgtcaat
    23221 ctacgttcaa tgaaatgatc gaagtaggat tggaaacagc gatagcagaa taagaaggaa
    23281 atgaaaaagc tgccctgcat taagtaataa gaacatccaa gcaaaaatag ctcctcatgt
    23341 ttttgacttg gctggactta ttattgcaaa tgtaagcttt tgaacaccgt ttaccaagag
    23401 gttgtgaatc tgccgtttaa ctctgagtca caaggaacaa tttcaaaaaa cagcttttca
    23461 tgtttgatga atattgtgac ttgtgaccga gaagttggca ggagaaaacc gtttatacga
    23521 aaaaagggac tgtgacaaat cgtgtcacag tccctttttt atagtgtttt ttataggtag
    23581 tattttttag caactgttaa gtcatcattt aattcataaa caagtggttg acctgttgga
    23641 atttcaagat ccatgatgtc ttcgtcagag ataccttcga tatgtttagc tagggcgcgt
    23701 aatgagttac catgtgcagc tactaaaaca gttttgttgt ctagtaatgc tggagcaatt
    23761 tcatcttgcc agaatggcaa agcgcgttct aatgtcactt tcaagttttc tccaccagga
    23821 atatcgcgtt tgtctaacat tgcgtaacga cggtcatttg ctgcagaacc ttcgtcagtt
    23881 tcttccatta atggaggaag agtatcgtaa gaacgacgcc agatgtgcac ttgttcgtcg
    23941 ccgtattttt cagctgtttc ttttttgttc aaaccttgta atttaccata gtgacgttcg
    24001 tttaagcgcc atgatttgat ttgtggtacc caaagttgat ctgaatattc taagatcaag
    24061 ttacatgttt tgattgcacg agttaaaaca gaagtgtaag caacatcaaa ttcgattcca
    24121 gcttctttga ttttgcgtcc gccttctttt gcttcttcga ttccttctgg tgctagattt
    24181 acatcagccc aaccagtaaa ttggttcaat gcattccatt cactaagacc gtgacgagaa
    24241 aaaactaatt ttggcatgtt tagctctcct tttaagatag gtttatattt ctgaaatttt
    24301 cagttctgtt ttattgtaca attttttgga agagatttcc atcgaaaaat tagataagat
    24361 tctatgagaa aacaagtaga gaatataaaa aaacgaacag tgcccacgca actgttcgtt
    24421 tttaggtatt agatgcataa ttgctcgatt tctttttcag gtaaattgat atatttataa
    24481 catgttccaa ctgaaacacc gcattttgaa gaaataaatt ggatcgtttc ttttttttca
    24541 taatagagac gtcggatttt tttgatcgtt tttgcatcga tttttggtct tccgccgatt
    24601 tttccttttt tacgagcttc atctaatcca actaatgtac gttctttgat caatgcgcac
    24661 tccatgtttg ctaggttttc cattaattgg aaatagattt ttcccatttc gcgcgtatcg
    24721 attccttcat caagactaat caagtgcagc ccgcgatctt tgaataattg cgtgagttct
    24781 gttaattgcc gcgtagattt gcctaatcga tgaagttggc aaatgactag tgcatcgcct
    24841 tgttgcatgt cagcgatcac tgactccaaa tgaacagcag tagaatcatt ttgttccact
    24901 tcatatgtct catggaaaat ttcttgacaa ccaaagtcag ataattgatc aacctgagtt
    24961 gttaaatcaa cgtcatttat ggttgtgcgt gcataaccaa taattttcat ggtgattcct
    25021 ttctagcagt gattttctaa ctggatataa cagtaaaaat gtagcataaa gtacgtcaaa
    25081 aagatagttt tttcgcaaat tttccgctgt ctttttttat tggatgatca agcagttttt
    25141 ctttatttac gacggattgc gtcaagaatc tcaacgcctt tttctgagcc gacaactact
    25201 ttagaaacca tgtttaggaa aagcccatgt tctactaccc caacaagttc atctaaatag
    25261 gtagctaagc tttctggctg ttcgatgact tctagatgca gatcaatgat gtaatgacca
    25321 ccatctgtgg ttaagacttc tccgttttcg tctgtacgca agacgggttg gaatcttttt
    25381 tcttcaaaaa tatgaagtag ctgttgggag ccataaggga tcacttcaac tgggagtggg
    25441 aacttcccaa gtttatcgac taatttagaa gaatcaacga tccaaatcgt ttcttttgag
    25501 tacgtagcga ctattttctc gaaaagcaaa gcagcgccgc cgcctttgat cccttgaaaa
    25561 tcagcactga tttcatcagc accgtctatt gtcaaatcga ctacggggac ttcatcaata
    25621 ctctttaacg gtattcctaa tttttccgct tgttcttttg tcgcatttga agtagtaact
    25681 cctgtgatac gtaaaccttc ttcacgcata cggcgaccaa tctcttctac catataatag
    25741 gcggttgaac cggtcccgag acctacgatc atcccgtcct ttacgaattt cgcggcttca
    25801 atgccagcta attgttttaa attcatcttt gcgtccacct ttttttagtt tgagcatcgt
    25861 gctttcctca ctattttagc gtgatttacg gtaaaaagat accattcttt tttattccaa
    25921 taaagctttt tcaaaaaaaa ttgcatctct tgttgacatg ttttttcatt cgtgatattc
    25981 taacaaaggt gctttatcta caggtttctg tgaagaaacg tgctgactgt ttatcaaagc
    26041 atatttgata aggatgcaga cattgcattt attttttatc acaaaaaaag aaccacctgg
    26101 atgtgtggaa ctagacgcaa atcgtgggaa ggaggaaaca aggaatgcct acaattaacc
    26161 aattagtacg taaacctcgt aaatcaaagg tggaaaaatc taattcacct gctttgaaca
    26221 aaggatataa cagctttaag aaaactcaaa ctaacgtaaa ctcacctcaa aaacgtgggg
    26281 tagcaactcg tgtgggtaca atgacaccta aaaaaccgaa ctcagcttta cgtaaatatg
    26341 cacgtgttcg tttgtcaaac ttaattgaag ttactgctta tatcccaggg attggtcata
    26401 acttacaaga acacagtgtt gtattattac gcggtggtcg tgtaaaagac ttaccagggg
    26461 tacgttacca tatcgttcgc ggtgctttag atactgctgg tgttaacgac cgtaaacaaa
    26521 gccgttctaa atacggaact aaaaaaccta aagcttaatt ttaattatta aacaaatgaa
    26581 acttgcttct actatataga agtaattact atgaacaata tttcggaagg aggagttacg
    26641 gatgccacgt aaaggtcctg ttgcaaaacg tgatgtttta ccagatccta tttataactc
    26701 aaaattagta actcgcttga tcaaccgtgt aatggttgac ggaaaacgcg ggattgcagc
    26761 taacattatc tataatgcct ttgacttgat caaagaatct acaggtaacg atccattaga
    26821 agtgtttgaa caagcaatga aaaacgttca acctgttctt gaagttaaag cacgtcgtgt
    26881 tgggggttca aactaccaag taccagttga agttcgccct gaacgccgcg ttactctagg
    26941 tcttcgttgg gtagtaaact acgctcgctt gcgcggtgaa cacacaatgg aagaacgctt
    27001 agcaaaagaa atcatggatg ctgcaaacaa cacaggtgct tctgttaaaa aacgtgaaga
    27061 cactcataaa atggctgatg ccaaccgtgc gtttgctcac tatcgctggt aagattctct
    27121 ctgtttggtc ggcttttata agtcgacctc agatagtgtc cttattttta gcaactagat
    27181 agtaaagaga ggagaaacaa ggagaatgac cagagaattt tctttacaaa acactcgtaa
    27241 tatcggtatc atggcccaca tcgatgctgg taaaacgact gctactgaac gtatcttgta
    27301 ctatactggt aaaatccata aaattggtga aactcatgaa ggagcttcac aaatggactg
    27361 gatggaacaa gaacaagaac gtggtattac gatcacttct gctgctacta cagcacaatg
    27421 gaagggtcac cgaatcaaca tcatcgacac accaggacac gtagacttca cagttgaagt
    27481 tgaacgttca ttgcgtgtac ttgatggtgc tgtaactgtt cttgatgcac aatcaggtgt
    27541 tgaacctcaa actgaaacag tttggcgtca agcaacaact tatggtgtac ctcgtatcgt
    27601 atttgcaaac aaaatggaca agatcggtgc tgacttctta tattcagtat caactttaca
    27661 tgaccgtttg caagcaaacg ctcacccaat ccaattacca attggtgcgg aagacgactt
    27721 cacaggaatt atcgaccttg taacaatgaa ggctgaaatg tatacaaacg atcttggaac
    27781 agagatcgaa gaaacagaaa ttcctgaaga ataccgcgaa ttagctgaag aatggcgcga
    27841 aaaattggtt gaagctgtag ctgaaactga tgaagaactt actttgaaat acttagaagg
    27901 tgaagaaatc actgaagctg agttaaaaga aggtatccgc cgcgcaacag ttaacgtaga
    27961 attctaccca gtactatgtg gttcagcatt taagaacaaa ggggttcaat tactattgga
    28021 cgctgttctt gattacttgc catcaccgtt agatatccca gctatcaaag ggattgaccc
    28081 taaaactgat gaagaagtag aacgtcctgc agacgacagc gctcctttct cagctttagc
    28141 atttaaagtt atgactgacc cattcgtagg tcgtttaact ttcttccgtg tgtactctgg
    28201 tgttttacaa tcaggttcat acgtacaaaa cgcaacaaaa ggcaaacgtg aacgtgttgg
    28261 tcgtatccta cagatgcatg ccaactctcg ttcagaaatc tctgaagttt acgctggaga
    28321 tatcgctgct gctgttggtt tgaaagacac aactactggg gacacactat gtgatgaaaa
    28381 gagtctagtt attcttgaat ccatggaatt cccagaacca gttatccaag tcgctattga
    28441 acctaaatca aaagctgacc aagataaaat gggtgtggct ttacaaaaac tatctgaaga
    28501 agatcctact ttccgtgctg aaacaaacgt ggaaacaggc gaaacaatca tcgctggtat
    28561 gggtgaactt cacttagata tcatcgttga ccgtatgcgt cgtgaattca aagttgaagc
    28621 taacgtaggt gcgcctcaag tatcttaccg tgaaactttc cgtgctggca ctcaagccga
    28681 aggtaagttt gtacgtcagt ctggtggtaa aggtcaatac ggtcacgtat ggattgaatt
    28741 tacaccaaat gaagaaggtg ctggtttcga atttgaaaat gcgatcgtcg gtggggttgt
    28801 tcctcgtgaa tacattccag ctgttgaaac tggtttgaaa gatgctatgg aaaatggtgt
    28861 cttagctggc tatccattag tagatatcaa agctaaactt tacgatggtt cataccatga
    28921 tgtcgattca aatgaaactg ccttccgtgt tgctgcttct atggcattac gtgcagcagc
    28981 taagaaagct aatccagtaa tccttgaacc aatcatggct gtagaagttg ttatccctga
    29041 agattattta ggtgatgtta tgggtcacgt aactgctcgt cgtggacgtg ttgaaggtat
    29101 ggaagctcgc gctggcggcc aacaagttgt tcgtgcgatg gttccacttg ctgaaatgtt
    29161 tggttatgct acaactcttc gttctgctac tcaaggtcgt ggtacattta caatgacatt
    29221 tgaccactac gaagatgtac caaaatcagt acaagaagaa atcattaaga aaaacggcgg
    29281 aaaagctgaa taatttcttg aaaccggata gtcctgcaaa gagattatcc aaagagacta
    29341 tatttttaat atagtttcgt gtaaaataat tatcgatgat atgggcatgt ggtgtacctt
    29401 gtgtacatca tatacctatc agacaaataa aaaatatatt gtatttttag gaggacattt
    29461 taaaatggca aaagaaaaat ttgaccgttc taaaccacac gttaacattg gtactatcgg
    29521 acacgttgac catggtaaaa ctacattaac agctgcaatc acaactgtac tatctaagaa
    29581 aaacggcggc caagctatgg cttacgatca aatcgatggt gctccagaag aacgcgaacg
    29641 cggaatcaca atctcaacag ctcacgttga gtatgaaaca gatacacgcc actacgctca
    29701 cgttgactgc ccaggacacg cggattacgt taaaaacatg atcactggtg ctgctcaaat
    29761 ggacggagct atcttggtag tttctgctgc tgacggccca atgcctcaaa ctcgtgaaca
    29821 catcctattg tctcgtcaag ttggtgttcc ttacatcgtt gtattcttga acaaagtaga
    29881 catggttgat gacgaagaat tactagaatt agttgaaatg gaagttcgtg acctattaac
    29941 agaatacgaa ttccctggtg acgatgttcc tgtagttgct ggatcagctt tgaaagctct
    30001 agaaggcgac gcttcatacg aagaaaaaat tcttgaatta atggctgcag ttgacgaata
    30061 catcccaact ccagaacgtg acaacgacaa accattcatg atgccagttg aagacgtgtt
    30121 ctcaattact ggacgtggta ctgttgctac aggtcgtgtt gaacgtggac aagttcgcgt
    30181 tggtgacgaa gttgaagttg ttggtattgc tgaagaaact tcaaaaacaa cagttactgg
    30241 tgttgaaatg ttccgtaaat tgttagacta cgctgaagct ggagacaaca ttggtgcttt
    30301 actacgtggt gttgcacgtg aagacatcca acgtggacaa gttttagcta aaccaggtac
    30361 aatcacacct catacaaaat tctctgcaga agtatacgtg ttgacaaaag aagaaggtgg
    30421 acgtcatact ccattcttca ctaactaccg tccacaattc tacttccgta caactgacgt
    30481 aacaggtgtt gttgaattac cagaaggaac tgaaatggtt atgcctggcg acaacgtaac
    30541 aatggaagtt gaattaatcc acccaatcgc tatcgaaaac ggtactaaat tctcaatccg
    30601 tgaaggcgga cgtacagttg gtgccggtgt cgttacagaa atcaaagctt aattcactta
    30661 agttttaaag taaagcaaca tttattcgga cgtaatcgct cggaacgaag gtcttaggat
    30721 ctttgttccg agctttttta tttattgcag gatcaggaaa aaagacagga gtattataaa
    30781 tagaagaaac acgaaataaa agagtcagta ttttctaaat gaaactttat gaaaacaaaa
    30841 tgttttttac aagaaaaagc cttgctttgt gagtttatat atattataat gacaaaggtg
    30901 cgtttacttt agtgaattgc acagacgtaa tcagaatgat ttcgtcggct aagaaacgag
    30961 aggttgcgac acgcccggac gctttgccac gaacgagcgt gacggaaatt ttcgtggagc
    31021 tatgtctact tttcaaaata ggcgaaggag ggaacaagat ggcaaaacaa aaaattcgta
    31081 tccgtttaaa agcgtatgaa caccgtattt tagatcaatc agcggataaa atcgtggaaa
    31141 ctgcaaaaag aactggagct agcgtgtctg gtccaattcc attaccaact gaacgcagtc
    31201 tttacacaat catccgtgcg actcataaat acaaagattc acgcgaacaa ttcgaaatgc
    31261 gtacacacaa acgtcttatc gacattgtga acccaacacc aaaaactgtt gatgctttga
    31321 tgaagctaga cttaccatct ggtgtaaaca tcgaaatcaa attataaaac taaaacaaaa
    31381 ataatggagg tgtactcatg accaaaggaa tcttagggaa aaaagtggga atgacacaaa
    31441 tcttcactga atctggtgaa ttaattccag taactgttgt agaagctact ccaaacgtag
    31501 tactacaagt gaaaacaatg gaaacggatg gctacgaagc tattcaagtt ggttaccaag
    31561 atatgcgtga agttttatca aacaaacctg cgaaaggtca tgttgcaaaa gcaaacacgg
    31621 ctcctaagcg cttcattcgt gaattcaaaa atgttgagct aggagaatat gaagtaggaa
    31681 aagaaattac agtagatgtt ttccaagcag gagacattat tgatgtaaca ggtactacga
    31741 aaggtaaagg attccaaggg gttatcaaac gccacggaca aagccgcgga ccaatggctc
    31801 acggttctcg ttaccatcgt cgtcctgggt caatgggtcc agttgcacct aaccgtgtat
    31861 ttaaaaacaa acgtcttgct ggccgtatgg gtggcaaccg cgtaactatc caaaacttgg
    31921 aagttgtacg tgtcgatgca gaaagaaatg ttatcttgat caaaggtaat gttcctggag
    31981 ctaaaaaatc tttaattact atcaaatcag ctgtgaaagc taaataattg taggaagaaa
    32041 ggaggaacta aggaatgccg aatgtagcat tattcaaaca agatggaagc caaaacggtg
    32101 aaatcacact taacgaagaa atcttcggaa ttgaacctaa tgaaagtgtt gtttacgatg
    32161 caatcgtaat gcaacgtgcg tcattaagac aaggaacaca cgcggttaaa aaccgtagcg
    32221 ctgttcgcgg cggcggacgt aaaccatggc gtcaaaaagg aacaggtcgt gcacgtcaag
    32281 gatctatccg ctcaccacaa tggcgtggag gtggcgtagt ctttggacca acaccacgtt
    32341 cttacagcta caaacttcct aaaaaagttc gtcgtttagc aatgaaatct gtattgtcag
    32401 aaaaagttgc tgaaaacaac ttggtagcaa tcgaaggctt gaacttcgac gcaccaaaaa
    32461 caaaagaatt caaacaagtt cttgctaact tatctatcga ctctaaagta ttggttgttc
    32521 tagaaaaagg taacgacttt gctgctttat cagcacgtaa cttaccaaac gtttctgtag
    32581 taacttctga taatgtgagt gtgctagacg ttgtatcaaa tacaaaagtg ttagcaacac
    32641 aaactgctct tactcaaatt gaggaggtgc ttgcataatg aacttactag acgtaatcaa
    32701 acgcccagtg atcactgaaa aatcaatgct tgccatggat gacaagaaat acacttttga
    32761 agtggacact cgcgcaaaca aaactttagt aaaacaagcg gtagaagctg cttttgacgt
    32821 taaagtcaaa aacgtgaaca tcgtgaacgt acgtgcgaaa ttcaaacgta tgggcaaata
    32881 tgcaggatat acaaaaaaac gtcgcaaagc gatcgttaca ttgactgaag attcaaaaga
    32941 aatccaatta ttcgaagctg ctgaataagc actaatagat aactcattaa attaggaggg
    33001 aaaaacgtgg cgattaaaaa gtacaaacct acctcaaatg gtcgtcgtaa catgacaggt
    33061 tctgattttg ctgaaatcac aacatcaaca cctgaaaaat cattgttaca accattgaaa
    33121 aaccatgctg gacgtaacaa caacggccgc atcactgtac gtcatcaagg tggcggacac
    33181 aaacgccaat atcgcgtgat cgacttcaaa cgtaacaaag ataacgttgt tgcaactgtt
    33241 caaacgatcg aatatgatcc aaaccgttca gctaacatcg cgttagtaca ctatgaagat
    33301 ggagtgaaag cttacatctt agcacctaaa ggactagaag tgggtatgaa acttgtttca
    33361 ggtccagaag cagatatcaa agtcggaaat gctcttccat tagaaaacat tccagttggt
    33421 actgttatcc acaacattga aatgaaacct ggtaaaggtg gacaattgat ccgttctgct
    33481 ggtacaagtg ctcaagtact tggtaaagaa ggcaaatacg tattgatccg cttgaactct
    33541 ggtgaagttc gtatgatctt agctgcatgc cgtgctacaa tcggttctgt aggtaacgaa
    33601 caacacgaat taatcaacat cggtaaagct ggacgctctc gttggatgcg taaacgccca
    33661 actgtacgtg gtagcgtaat gaaccctaat gatcacccac acggtggtgg tgaaggtaaa
    33721 gctcctatcg gacgtaaagc accagtttct ccttggggtc aaccagctct tggttacaaa
    33781 actcgtaaca aaaaagctaa atcagacaaa cttattgttc gtcgtcgcaa aaataaataa
    33841 ttactttgac catgtgtcgc acattttgag aggaggttca ccatgggtcg tagtttaaag
    33901 aaaggacctt ttgtcgatga gcatttaatg aaaaaagttg aagctcaagc aggggttgaa
    33961 aagaaaaaag ttatcaaaac ttggtcacgc cgttcaacag ttttcccaag ttttgttgga
    34021 tataccatcg cagtttatga tggacgtaaa catgtaccag tttacatcca agaagacatg
    34081 gtaggacaca aattaggtga atttgcacct acaagaactt atcgtggaca tgctgctgac
    34141 gataagaaaa cgaaacgcta atttgagggg aggataaatc caaatggcag aacaaatcac
    34201 atcagctaaa gcaactgcaa aaacagttcg cacttcacct cgaaaagccc gtttagtaat
    34261 tgaccttatc agaggtaaaa gtgtcgcaga tgcaatttca atcttgaaat tcacaccaaa
    34321 caaatctgct ggaatcattg aaaaagtatt gatgtcagca gttgcgaatg cagaaaataa
    34381 ctttgactta gatgttgaaa acttggtagt aagcgaagct tttgttaacg aaggacctac
    34441 aatgaaacgt ttccgtcctc gtgcaaaagg atcagcttct ccaatcaaca aacgtacaag
    34501 tcatatcacg gtagtcgtat cagaaaaata aggagggata atcagtgggt caaaaagtac
    34561 atccaattgg aatgcgtgta ggcatcatcc gcgactggga tgctaaatgg tatgctgaaa
    34621 aagagtatgc tgagttctta cacgaagatt taagaatccg taaatttatt tcaactaaac
    34681 ttgctgatgc tgctgtatca acagttgaaa tcgaacgcgc aacaggtcgc gtaaacattt
    34741 caattcacac agctaaacca ggtatggtta ttggtaaagg cggttctgaa gtcgaaagcc
    34801 taagaaaaga attaaacaaa ttaactggta aaagaatcca tatcaatatc gtggaaatca
    34861 aaaaaccaga tctagatgca aaattagtag gtgaaggaat cgcacgtcaa ttagaaaacc
    34921 gtgttgcttt ccgtcgcgct caaaaacaag ccatccaacg ctcaatgcgt gctggcgctc
    34981 aagggatcaa aactcaagta tctggtcgtc taaacggagc agatatcgct cgttcagaag
    35041 gatactcaga aggaactgtt cctcttcata ctttacgtgc ggacattgat tacgcatggg
    35101 aagaagcaga tacaacatac ggaaaactag gagttaaagt gtggatctat cgtggagaaa
    35161 ttcttccaac taaaaaaaac actgagaaag gagggaaata atcatgttag tacctaaacg
    35221 tgtaaaacac cgtcgtgaat tccgcggaaa aatgcgcggg gaagctaaag gcggaaaaga
    35281 agtagcattc ggtgaatacg gtttgcaagc tgttgattca cattggatca caaaccgcca
    35341 aatcgaagct gctcgtatcg caatgactcg ttacatgaaa cgtggtggga aagtatggat
    35401 taaaattttc cctcacaaat cttatactgc caaagcaatt ggggtacgta tgggttctgg
    35461 taaaggggca cctgaaggat gggttgcacc agtaaaacgt ggtaaaatca tgtttgaaat
    35521 cgcaggcgtt cctgaagaag tagctcgtga agcgttacgt ctagcttctc acaaattacc
    35581 aatgaaaact aagatcgtaa aacgtgagga aatgggtggt gaatcgaatg aaggttaaag
    35641 aaatcagaga attaaccact gccgaaatgc ttgatcaaga aaaacaatta aaagaagaat
    35701 tgtttaatct tagattccaa ttagcaacag gtcaattaga aaacactgca cgtattaaag
    35761 aagtacgtaa atcgattgca cgcatcaaaa ctgtgttgcg tgaacaagct aagtaattat
    35821 ggaaggaggc cattaagcgt atgactgaag gaagaaatca acgcaaagtt tatcaaggtc
    35881 gcgtggtttc agacaaaatg gataaaacaa ttaccgttgc tgtcgaaaca aagaaaaacc
    35941 accctatcta tggcaaacgc atgaactatt ctaagaaata caaagtgcat gatgaaaaca
    36001 acacagcaaa agttggcgac atcgtgagaa ttatggaaac tcgtccatta tcagctacaa
    36061 aacgtttccg tttgttggaa gtagtcgaag aagcagttat tatctaataa cacgagattt
    36121 tcctatatag attgaatgca aaatctgaaa ggaggataca caacgtgatc caagcagaaa
    36181 gtcgtttaaa agtcgctgat aactcaggcg cacgtgaaat tttgacgatc aaagtccttg
    36241 gtggatctgg tcgtaaaact gctaacatcg gtgatgtgat cgttgctacg gttaaacaag
    36301 caacgccagg tggggttgtc aaaaaaggtg acgtcgttaa agccgttatc gttcgaacaa
    36361 aatcaggagc tcgtcgtaca gacggttcat atattaaatt tgatgaaaat gctgcagtga
    36421 ttattcgtga tgataaaagc cctcgtggaa cacgtatctt tgggccagtt gcacgtgaac
    36481 tacgtgaaaa caacttcatg aagatcgttt cactagcacc agaagtttta taatcttgag
    36541 acacttatca aaggaggtgc gaaacagtta tgtttgttaa aaaaggcgat aaagttaaag
    36601 ttatcactgg aaaagacaaa aacaaagaag gcgttgtact agcagcgttc ccgaaaaaag
    36661 ataaagttat tgtcgaaggt gtaaatgtcg ttaaaaaaca ccaaaaacct tctcaagctg
    36721 ctcctcaagg tggaattgtt gagatggaag cgccaattca tgtttctaac gtgatggtgg
    36781 ttgactctac tggtgtagct ggccgagttg gctacaaaga agtagacggc aaaaaagtcc
    36841 gtgtttctaa aaaaaccggt gaagttttag ataaataaaa gtttaaggaa ggagggtctt
    36901 actgaatgaa ccgcctaaaa gaaaaatatc ttaaagaagt aactccatca ttgatggaaa
    36961 aatttaacta tagctctgtt atgcaaacac ctaaagttga aaaaatcgtc atcaacatgg
    37021 gtgttggtga tgcggtatca aacgctaaaa acttagataa agcagttgaa gaattaactt
    37081 tgatcactgg tcaaaaacca atgattacaa aagctaaaaa atcaatcgct ggattccgtt
    37141 tacgtgaagg aatgccaatt ggtgcaaaag ttacattgcg cggagaaaga atgtacgaat
    37201 tcttagacaa attggtttct gtttcactac cacgtgtacg tgacttccat ggtgtaagta
    37261 aaaaagcctt tgacggtcgt ggaaactata ctttaggtat taaagaacaa ttgatcttcc
    37321 cagaagtaga ttatgattta gtagataaag tacgtggtat ggacatcgtt attgtaacaa
    37381 cagcaaactc tgatgaagaa tctcgtgagt tgttgacaca attaggcatg ccattccaaa
    37441 aataattaaa ggaggcgaac tacgtggcta aaaaatcaat gattgctaaa aacaaacgtc
    37501 ctgcaaaatt ttcaacacaa gcttatactc gttgcgaacg ttgcggacgt ccacattcag
    37561 tttatcgtaa gttccatctt tgccgtattt gcttccgcga acttgcctat aaaggtcaaa
    37621 ttcccggcgt gaagaaagct agctggtaaa aagtaaattc gcataagaag gaggtaaaag
    37681 atcaatggtc atgacagatc caattgcaga ttttctaact cgtatccgta acgcaaacat
    37741 ggttaaacat gagtctttag aagtacctgc atcaaaaatt aaacgtgaca tcgctgaaat
    37801 cttgaaacgt gaaggtttcg ttcgtgatgt tgaatacatc gaagatgaca aacaaggcgt
    37861 gatccgtgtt ttcctaaaat ttggtaaaaa cggcgaacgt gttatcacaa acttaaaacg
    37921 tatctctaaa ccaggtttgc gtgcttatgt caaagctgac gaagtaccaa aagtattgaa
    37981 cggtttaggt atcgcaatca tttcgacttc tgaaggtgtt gtcactgata aagaagctcg
    38041 tgaaaaaaat atcggcggcg aagttatcgc ttacgtatgg taatcaagta gaatctacaa
    38101 ggaggtgtct ttaagtgagc cgtatcggaa ataaagtcgt tgttatccct gaaggagtaa
    38161 cagttactca agacggaaac aacgttacag ttaaaggacc aaaaggtgaa ttgactcgta
    38221 ctttttcagc agatatcaaa atgaatatcg aaggaaacga agtaactttc actcgtccaa
    38281 acgacagtaa agaaatgaaa acaattcatg gaacaactcg tgcgaacttc aacaacatgg
    38341 ttgtaggtgt cagcgaaggt ttccaaaaag cgttagaact aatcggggtt gggtaccgtg
    38401 cacaattaca aggatcaaaa cttgtattga acgttggtta ctcacatcca gtagaaatca
    38461 caccaccagc tggagtaact gttgaagtac cagctaacac acaagtaatt gttaaaggtg
    38521 cgaacaaaga agaagttggc gaattagctg caaacattcg tggaactcgt cctccagaac
    38581 cttataaagg taaaggtatt cgttatgttg gcgaatatgt acgccgtaaa gaaggtaaaa
    38641 ctggtaaata atcgtttaga aaaattgcat agaagccagc agcaggaaaa aggcatttgg
    38701 ctgaagttta ccttcaaaaa aatacatttt tacttgtgcc gcagcttcta tttttcgaaa
    38761 cgtatttact gcttgaaata gtagtacaat aaaaataaag aggtgacaat tgtgattaca
    38821 aaaccagata agaacaaaac acgtcaaaag agacatcgtc gtgtacgtaa caatatctct
    38881 ggtactgctg agcgcccacg cttgaacgtt tttcgttcta acaaaaacat ctacgctcaa
    38941 attattgatg acgtagcggg tgtaacgcta gcaagtgcct ctaccttgga taaagaaatt
    39001 tcaggtggaa caaaaacaga agcagcacaa gctgtcggta aactagttgc tgaacgtgca
    39061 gctgaaaaag gcattaaagt agtagtcttt gaccgtggtg gataccttta ccatggccgt
    39121 gtgcaagctt tagctgaagc tgctcgcgaa aatggactag aattttagaa gaaggaggaa
    39181 taccattcat ggtttatatc gatccaaaac atttggaatt agaagaccgc gttgttgcaa
    39241 ttaaccgtgt aacaaaagtt gttaaaggtg gacgtcgtct acgttttgct gctttagttg
    39301 ttgtcggtga caaaaacgga cacgtaggat ttggtacagg taaagctcaa gaagttcctg
    39361 aagcaatccg taaagcaatt gaagatgcga agaaaaactt agttgaagta cctatggttg
    39421 gttcaacaat cccacacgaa gtaatcggtg cttttagtgg tggtagaatc ctaatgaaac
    39481 ctgctgtaga aggttctggg gttgctgctg gtggaccagt tcgtgccgta ttagaattag
    39541 ctggggtagc agatatcact tctaaatctt taggctcaaa tacaccaatc aacgttgttc
    39601 gcgcaacagt tgaaggtttg aaacaattga aacgtgcaga agaagttgca gaacttcgcg
    39661 gcaaatcagt tgaagaatta atcggataag gaggacaaca ataatggctg aattaaaagt
    39721 tactttaaaa cgcagtatta tcggacgtcc tcaaaaccaa cgcgatacag ttaaagcgtt
    39781 aggtcttaca aagattaaca gttcagtggt taagccttct aatgaagcaa ttaaaggcat
    39841 gatcaatact gtttctcacc tagtggacgt tgaagaaatt taatttagaa aaatcataac
    39901 tagaacatta aggaggtgcc aaacctatga aacttcatga attaaaacct gctgaaggtt
    39961 cacgccaagt acgtaaccgt gttggtcgtg gtacttcatc tggtaacgga aaaactgccg
    40021 gacgcggaca aaaaggacaa aaagctcgtt caggcggcgg tgtgcgttta ggatttgaag
    40081 ggggacaaac accattgttc cgtcgtttac caaaacgagg attcacaaac gtgaaccgta
    40141 aagactatgc agtcattaat cttgatgtat tgaaccgctt tgaagacgga gctgaagtaa
    40201 ctcctgttgc tttagttgaa gctggaatcg tgaaaaacga aaaagctgga atcaaagtat
    40261 tagctaatgg ggaactaaac aagaaattaa ctgtgaaagc agcaaaattc tctaaagcag
    40321 ctcaagaagc aattgaagcg gctggtggat caatcgaggt gatctaatgt ttagactatt
    40381 aaaagatgct tttaaagtaa aagacattag atcgaaaatc ctatttactg tatttgtgtt
    40441 gtttgtattt cgcctaggtg cacatattac tgtaccaggc gtaaatgctg cttcattgca
    40501 aaacctttct tctttgccat ttttcaacat gttgaacttg gtaagtggta gtgcgatgca
    40561 aaacttttcc attttctcaa tgggggtttc accatacatt actgcttcta tcgtcattca
    40621 gcttcttcaa atggacatcg tgcctcgatt tgttgaatgg tcgaaacaag gggaagttgg
    40681 tcgtaaaaaa ctgaatcaag cgactcgata tctgacatta gtattggcat ttatccaatc
    40741 tgtcggtatc actgctggat tcaatcaata cgctcaatta ggtttcgtga ataatcctaa
    40801 cgtagcaact tacgtaacga tcggtttgat tctaacagcc ggtacgatgt ttgttacttg
    40861 gctaggagaa cagatcacag aaaaaggtat cggaaatggc gtatcaatga tcatttttgc
    40921 aggtatcatt tctagattgc ctgaaagtat cagagaaatt tatgaagatt actttataaa
    40981 tattgatcct tcagacatat ggaaatctgc tatttttgtg gccattttac tagtagcggt
    41041 cttgttgatc gtgacattcg taacattttt ccaacaagct gaacgcaaga tcccgatcca
    41101 atatacgaaa cgcgtagctg gcgcgcctac aagcagctat ctgccgttga aagtaaatgc
    41161 agctggagtt attccagtta tctttgctag ttcgtttatt gctacgccaa atgctgtttt
    41221 acaagcattg agcggtagat tcggttctga aaactggtac aaagtgatga tggagatctt
    41281 taactataat acagcacctg gtgctacgat ttatacgatt ctgatcgttg ccttcacttt
    41341 cttctacgcc tttgttcaag taaaccctga gaaattagcg gaaaacttgc aaaagcaagg
    41401 aagttatata ccaagcgtac gccctggtaa ggggactgaa gagtatgtct caagattact
    41461 gatgcgttta agtacagttg gtgcagtctt ccttggttta gtggcattgc ttccaatcat
    41521 tgctcaaatg gtttgggatc ttccacaatc aatcggatta ggcggtacaa gcttactgat
    41581 cgttatcggt gtagctttag aaactgcgaa acagttagaa ggcttaatgc tgaaacgtaa
    41641 atatgttgga ttcattgagt aagaaagcgt gttgaggttt cccctcaaca cacgttctca
    41701 cacatttatt aggagggaaa acaatgaacc tcattttaat ggggctgcca ggcgcaggta
    41761 aaggaacaca agctgaaaaa atcattgatg cttatggtat tccgcacatc tcaacaggag
    41821 atatgttccg cgcagcgatg caaaacgaaa cagctcttgg cctggaagca aagtcttata
    41881 tggataaagg tgcattagtt cctgatgaag taacaaatgg aatcgtgaaa gagcgactag
    41941 ctgaaccaga tacagagaaa ggcttcttat tagatggctt tccacgtacg ctagatcaag
    42001 caaaagctct ggacgcaatg ctaaaagatt tgaataaaaa aattgatgct gtcatcgata
    42061 tccatgttgg tgaagaaatc ttggtcgaac gtctagccgg ccgatttatc tgcagcaatt
    42121 gtggagcaac ttaccataaa attttcaacc ctacaaaagt tgaagacaca tgtgatcgtt
    42181 gcggtgggca tgaattctat caaagagaag atgataaacc tgagacggtt aaaaatcgtc
    42241 tggctatcaa catcaaaaat agtgaaccaa tcttggctta ttataaagaa caaggtttgc
    42301 taaacactat tgatggtgct cgtgaaatcg atgctgtatt tgcagatgta caaaaaatca
    42361 ttgaaaaata gcacgattgc ccgtagccga cttgcattat gcagttactc ttgtcaagtt
    42421 caataaatta tgatagaata tgtcagtccg acttctagag aatcctctag atgatccatt
    42481 ttgagaattt tgaggtggga actgcagttt ccgccatttt atcgtgtgaa aatgcgaaac
    42541 aagtacaagg aggtactgtg cgtggcaaaa gaagatatga ttgaagtcga aggtacagtc
    42601 gtcgaaactt tgccgaatgc aatgtttaaa gttgaactag aaaacggaca ccaagttctt
    42661 gctactgttt caggtaaaat tcgtatgcat tacatccgta tcttacctgg tgacaaagta
    42721 acggttgaat tgtctccata cgatcttaac cgtggccgta ttacttatcg ctttaaataa
    42781 ttgtactccg taaaactaca ggaggtatta tcatgaaagt aagaccatca gtaaaaccaa
    42841 tgtgcgaaca ttgtaaagta attcgtcgta aaggacgcgt tatggtgatt tgcccagcaa
    42901 atccaaaaca taaacaacgt caaggataat tggaggtgct ataaatatgg ctcgtattgc
    42961 aggagtagat attcctcgtg ataaacgtgt agtagtttca cttacttata tctatggtat
    43021 tggtaacact actgctaaaa agatcttagc tgatgctggt gtatctgaag atgttcgcgt
    43081 tcgtgaatta acgaatgaac aaacagatgc tatccgtgca gaagtagata aattaaaagt
    43141 tgaaggggat cttcgtcgtg aagtgaactt gaacatcaaa cgtttgatgg aaatcggttc
    43201 ttaccgtgga atccgtcatc gccgtggatt gcctgttcgt ggacaaaaca cgaaaaacaa
    43261 tgcacgtact cgtaaaggcc cagctcgcgc tatcgctggc aagaaaaaat aattcttaag
    43321 tgaaggaggt taaaacttca tggcagcaaa aaaagtgaat cgtaaacgcc gtgtgaagaa
    43381 gaatatagaa gcaggtattg cacatatcca ttctacattc aataacacta tcatcatgat
    43441 cactgatact cacggaaatg cattagcgtg gtcatcagct ggttcattag gtttcaaagg
    43501 aagcaaaaaa tcaactcctt ttgccgctca aatggcagca gaaactgcta caaaagcagc
    43561 aatggaacac ggattaaaga ctgttgaagt aacagttaaa ggacctggtt ctggacgtga
    43621 agcagcaatt cgttcattac aagctacagg tttagaagtg actgcaattc gtgacgtgac
    43681 tccagttcct cataatggtg cccgccctcc aaaacgccgt cgtgtttaat gagtgccgct
    43741 cattttgagt tttatcagta acgtcattga acaagacact tttcgttttg aaaggggtaa
    43801 agataagaat gattgagttc gaaaaaccaa gaatcgcaaa aattgatgaa gaaaaagatt
    43861 atggcaagtt catcgttgaa cctcttgaaa gagggtatgg gactacttta gggaattccc
    43921 tacgtcgtat tttattatct tctttgccag gtgctgccat cactaatatt caaatcgatg
    43981 gtgtactgca tgaattctct accatcaaag gtgttagaga agacgtcact caaatcatct
    44041 tgaacattaa aggcttagcg ctgaaaatgt atggccaaga agaaaaaacc cttgaaatcg
    44101 atattaccgg acctgctaca gtaactgctg gcgatattat cgttgatagt gatgtagaaa
    44161 ttcttaacaa agatatgtac atctgtagtg tctctgaagg agctacgttc catgctcgct
    44221 taacagtgaa acctggacgt ggttatgttc aagcagatga aaataaaaaa gaagatatgc
    44281 caattggcgt tcttccagtc gattcaatct acacacctgt tcgtcgtgtg aactatcaag
    44341 ttgagaacac acgtgttgga caccgtgaag attttgataa attgacgatg gagatttgga
    44401 cagatggttc aattgaaccg ttggaagcaa tgagtttgtc tgcgaaaatc atgactgaac
    44461 atttggatat ttttgtgaac ttaactgatg aagcaaaaaa tgctgaaatc atggtcgaaa
    44521 aagaagaaac acaaaaagaa aaaatgttgg aaatgacgat cgaagagtta gacttgtctg
    44581 ttcgctctta caattgtttg aaacgtgcag gaatcaatac tgtacaagaa cttacaaata
    44641 aatctgagcc tgaaatgatc aaagtccgta acttaggacg taaatcatta gaagaagtta
    44701 aagcaaaatt gcacgactta ggtttaggtt tacgtaaaga agattaaaac cttttacgaa
    44761 ggagggaaac caacgtgagt tatcgtaaac taggacgcac atctagccaa cgtaaagcga
    44821 tgttgcgtga tttaacaact gatttattaa tcaacgaacg tattgtgact actgaagctc
    44881 gtgcgaaaga agttcgctca actgcagaaa aaatgatcac tttaggtaaa cgcggagatt
    44941 tgcatgcacg tcgtcaagct gcggctttcg tacgtaatga agttgccagc gtgcgtgaag
    45001 aaaatgagga catcgtcatt gaatcagctt tacaaaaact gttcaacgat attgcacctc
    45061 gttacgcaga acgtcaaggc ggatacacac gcattttgaa gacagaacca agacgcggtg
    45121 atggtgcacc aatggttgtc ttagaacttg tctaaaatca cactaataac atcactttat
    45181 agatgattct tttagagtgt tatgatgttg gaagatattt ttccgagtct agctcagcgc
    45241 tgctcctcaa gcgaatatcg cttggggagt aagcactcat cataaagtgt ttttttatac
    45301 cttcaattac ctgtcatgac agatatgttt gaaggcataa aaaaacattt taagcgagaa
    45361 ataattgaaa attttgtctt tttttgatag tgttagtagg aactttagga tgaagaagga
    45421 gtaatcatgg agccaattat tgaattagaa aagataaatt ataaatacca gccagatgat
    45481 cttcgccccg cattgaaaga tgtctccttt acaattgata aaggtgagtg gatcgctatt
    45541 attggacaca atggttcagg aaaatcgaca cttgccaaaa cgatcaatgg attgcttctg
    45601 ccagaatcag ggatcgtgaa agtgggaaat caaatattgg atgaagaaaa tatttggacg
    45661 atccgacaga tggttggaat ggtttttcaa aatccagaca atcagtttgt tggttcgaca
    45721 gtagaagatg acgttgcttt tggtcttgaa aatcaaggga ttcccagaga agaaatgcta
    45781 gtgcgtgtga aagatgcact tgagaaagtt agaatggctg agtttgcttc acgcgagcct
    45841 gctcgtttat caggagggca aaaacaacgt gttgccattg ccggcgtggt tgccttacga
    45901 cctgatatca ttattttaga tgaagcaacc agtatgctag atccagaagg tcgtgaagaa
    45961 gtgatctcta cgatcaagaa gatcaaagaa gaaagtcaat tgaccgttat atctatcact
    46021 catgatattg atgaagcagc taatgccaat cggatactag tgatgagaca aggcgaactt
    46081 gtccgtgaag gtacaccaaa agaaattttt tctgctggtc ctgaactgat cgacttaggt
    46141 ctagatctac catttcctga aaaactgaag agcgcactga aagaacgtgg ggttgatgtg
    46201 cccagtgaat atatgacaga agaaaggatg gtggattggt tatggacatc cgttttgaac
    46261 aagtagactt cacttatcag ccgaatacac catttgaaca aagagcctta tttgacatca
    46321 atatgacgat caaagaaaac agttatactg cattagtagg gcataccgga agcgggaaat
    46381 caaccttact tcagcatttg aatgcgcttg ttaagccaac aagcggtaca gtccatattg
    46441 gagaacgaga tattcagccg gatacggata ataagaattt aaagcccatc cgaaaaaaag
    46501 ttggcatcgt ctttcagttt ccagaagcac agctattcga agaaacggta gcaaaagaca
    46561 ttgctttcgg tcctaaaaac tttggtgtca gcgaagaaga agcactagtc ctagcaaaag
    46621 aaacattaga acaagttggg ctggatgaaa gctatttgga acgttcgcca tttgaacttt
    46681 caggagggca aatgcgtaga gtagcaatcg ctggtgtgct tgccatgagg ccggaagtac
    46741 ttgtattgga tgaaccaacg gcaggacttg atccccaggg cagaaaagaa atgatggaga
    46801 tgttttggcg tcttcataag gaacagcaga tcactattgt attagttacg catttaatgg
    46861 atgatgtagc caattttgca gattatgtct atgtgttgga aaagggacga atcgttaata
    46921 gcggggagcc gcaagaggtc tttcaaaaca ttgaatggct aaaagaaaaa caactaggcg
    46981 tacctactgc caccgagttt gcagaagaat taatggctaa agggatgaac tttgctacct
    47041 tgcctttgac agctgaagaa ttagcagacg ctatcgtcca ttatgctgga ggaaatgccc
    47101 atgatgaata aattgatatt tggtcgttat atgcctggag attccttgat ccatcgattg
    47161 gatccccgag ctaaattatt agccagtttt tattttatcg gaatcatttt tttagcaaat
    47221 aattgggaaa cgtacctttt gttggcattt ttcacattac tttctgtctt cttgtcaaaa
    47281 gtagatttga aattttttat ccgaggcatt cgcccattga tctggctaat cctcttcact
    47341 gtagctttgc aggtcttgtt tacaagtggc ggagaaactt attggagttg gggtgttttc
    47401 catatcactg aatttggtgt gatgaacggc ctctttattt tctgtcgatt cgtgttgatc
    47461 atttttatgt cgactctact cacgcttact acaccgcctt tggaattatc ggatgcgatt
    47521 gagtacttgc ttcgtccatt gaaagccgta cggttcccag tccacgaaat ttcattgatg
    47581 ctgtcgattg ctcttcgttt tgtgcctaca ttgatggacg aaacagaaaa gatcatgaac
    47641 gctcagcgcg ctcgcggtgt tgattttggt gaaggaaacc tagttcagaa aatgaaagca
    47701 gtcattcctt tgctgattcc tttatttgtc agcagcttca accgtgcgga agatcttgcg
    47761 actgctatgg aagcaagagg ctacaaaggt ggggaaggaa gaacgaaata tcgtgtattg
    47821 cattggcata atagagacac gatggttatc attgcttttg tattattaac tgtgctactg
    47881 atctttttaa gaggataaac gttcagtggg ccgctatcta ttcttttctt gctgatctgt
    47941 ttgcaagaaa ggatgcaagc agtgtatgac aagaaatatt agggtctgtg gcagactaag
    48001 gtcacaggcc tctttctttt atctgaaaag aaagaacgaa aaaatt
//
LOCUS       DASNNU010000009        46069 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.009, whole genome shotgun sequence.
ACCESSION   DASNNU010000009 DASNNU010000000
VERSION     DASNNU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.009"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            41..922
                     /locus_tag="U8116_000503"
     CDS             41..922
                     /locus_tag="U8116_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8433520.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            935..2545
                     /locus_tag="U8116_000504"
     CDS             935..2545
                     /locus_tag="U8116_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEN8433521.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2622..3281)
                     /locus_tag="U8116_000505"
     CDS             complement(2622..3281)
                     /locus_tag="U8116_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN8433522.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3564..4229)
                     /locus_tag="U8116_000506"
     CDS             complement(3564..4229)
                     /locus_tag="U8116_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEN8433523.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4376..4981)
                     /gene="dhaL"
                     /locus_tag="U8116_000507"
     CDS             complement(4376..4981)
                     /gene="dhaL"
                     /locus_tag="U8116_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEN8433524.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5009..6019)
                     /gene="dhaK"
                     /locus_tag="U8116_000508"
     CDS             complement(5009..6019)
                     /gene="dhaK"
                     /locus_tag="U8116_000508"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEN8433525.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5994..6440)
                     /gene="dhaM"
                     /locus_tag="U8116_000509"
     CDS             complement(5994..6440)
                     /gene="dhaM"
                     /locus_tag="U8116_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEN8433526.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6543..6863
                     /locus_tag="U8116_000510"
     CDS             6543..6863
                     /locus_tag="U8116_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEN8433527.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6975..7844)
                     /locus_tag="U8116_000511"
     CDS             complement(6975..7844)
                     /locus_tag="U8116_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN8433528.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7940..9244)
                     /locus_tag="U8116_000512"
     CDS             complement(7940..9244)
                     /locus_tag="U8116_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN8433529.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9241..9750)
                     /locus_tag="U8116_000513"
     CDS             complement(9241..9750)
                     /locus_tag="U8116_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433530.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9925..12060)
                     /locus_tag="U8116_000514"
     CDS             complement(9925..12060)
                     /locus_tag="U8116_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN8433531.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12143..13798)
                     /locus_tag="U8116_000515"
     CDS             complement(12143..13798)
                     /locus_tag="U8116_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN8433532.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13800..14114)
                     /locus_tag="U8116_000516"
     CDS             complement(13800..14114)
                     /locus_tag="U8116_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN8433533.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14284..14544
                     /locus_tag="U8116_000517"
     CDS             14284..14544
                     /locus_tag="U8116_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEN8433534.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14558..14872
                     /locus_tag="U8116_000518"
     CDS             14558..14872
                     /locus_tag="U8116_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEN8433535.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15602..16156)
                     /locus_tag="U8116_000519"
     CDS             complement(15602..16156)
                     /locus_tag="U8116_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEN8433536.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16282..18211)
                     /locus_tag="U8116_000520"
                     /pseudo
     CDS             complement(16282..18211)
                     /locus_tag="U8116_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18517..19233)
                     /locus_tag="U8116_000521"
     CDS             complement(18517..19233)
                     /locus_tag="U8116_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN8433537.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19234..21057)
                     /gene="glpO"
                     /locus_tag="U8116_000522"
     CDS             complement(19234..21057)
                     /gene="glpO"
                     /locus_tag="U8116_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEN8433538.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21071..22567)
                     /gene="glpK"
                     /locus_tag="U8116_000523"
     CDS             complement(21071..22567)
                     /gene="glpK"
                     /locus_tag="U8116_000523"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEN8433539.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22703..24163)
                     /locus_tag="U8116_000524"
     CDS             complement(22703..24163)
                     /locus_tag="U8116_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8433540.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24153..25448)
                     /locus_tag="U8116_000525"
     CDS             complement(24153..25448)
                     /locus_tag="U8116_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN8433541.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25780..26457)
                     /locus_tag="U8116_000526"
     CDS             complement(25780..26457)
                     /locus_tag="U8116_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEN8433542.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26487..27578)
                     /locus_tag="U8116_000527"
     CDS             complement(26487..27578)
                     /locus_tag="U8116_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEN8433543.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27679..28113)
                     /locus_tag="U8116_000528"
     CDS             complement(27679..28113)
                     /locus_tag="U8116_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN8433544.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28697..30154)
                     /gene="ascB"
                     /locus_tag="U8116_000529"
     CDS             complement(28697..30154)
                     /gene="ascB"
                     /locus_tag="U8116_000529"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN8433545.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30151..32046)
                     /locus_tag="U8116_000530"
     CDS             complement(30151..32046)
                     /locus_tag="U8116_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN8433546.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32104..33879)
                     /locus_tag="U8116_000531"
     CDS             complement(32104..33879)
                     /locus_tag="U8116_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN8433547.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33929..35425)
                     /locus_tag="U8116_000532"
     CDS             complement(33929..35425)
                     /locus_tag="U8116_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN8433548.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35619..37241)
                     /locus_tag="U8116_000533"
     CDS             complement(35619..37241)
                     /locus_tag="U8116_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN8433549.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37569..38882)
                     /locus_tag="U8116_000534"
     CDS             complement(37569..38882)
                     /locus_tag="U8116_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN8433550.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39127..39351)
                     /locus_tag="U8116_000535"
     CDS             complement(39127..39351)
                     /locus_tag="U8116_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433551.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39371..39793)
                     /locus_tag="U8116_000536"
     CDS             complement(39371..39793)
                     /locus_tag="U8116_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEN8433552.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39935..40645)
                     /locus_tag="U8116_000537"
     CDS             complement(39935..40645)
                     /locus_tag="U8116_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433553.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40663..41160)
                     /locus_tag="U8116_000538"
     CDS             complement(40663..41160)
                     /locus_tag="U8116_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8433554.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41197..41649)
                     /locus_tag="U8116_000539"
     CDS             complement(41197..41649)
                     /locus_tag="U8116_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN8433555.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41784..42389)
                     /locus_tag="U8116_000540"
     CDS             complement(41784..42389)
                     /locus_tag="U8116_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN8433556.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42839..43459)
                     /locus_tag="U8116_000541"
     CDS             complement(42839..43459)
                     /locus_tag="U8116_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN8433557.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43461..44330)
                     /locus_tag="U8116_000542"
     CDS             complement(43461..44330)
                     /locus_tag="U8116_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEN8433558.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44353..44760)
                     /locus_tag="U8116_000543"
     CDS             complement(44353..44760)
                     /locus_tag="U8116_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN8433559.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44779..46068)
                     /locus_tag="U8116_000544"
     CDS             complement(44779..46068)
                     /locus_tag="U8116_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN8433560.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        12926 a         9837 c         7890 g        15416 t
ORIGIN      
        1 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
       61 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
      121 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
      181 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
      241 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
      301 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
      361 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
      421 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
      481 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
      541 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
      601 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
      661 actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
      721 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
      781 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
      841 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
      901 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
      961 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     1021 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     1081 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     1141 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     1201 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     1261 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     1321 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     1381 ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg
     1441 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     1501 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     1561 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     1621 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     1681 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     1741 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     1801 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     1861 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     1921 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     1981 gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     2041 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
     2101 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
     2161 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
     2221 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
     2281 aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt
     2341 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
     2401 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
     2461 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
     2521 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
     2581 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
     2641 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
     2701 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
     2761 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
     2821 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
     2881 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
     2941 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
     3001 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
     3061 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
     3121 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
     3181 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
     3241 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
     3301 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
     3361 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
     3421 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
     3481 ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt
     3541 tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt
     3601 ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga
     3661 tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct
     3721 tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct
     3781 ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc
     3841 gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg
     3901 tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg
     3961 tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc
     4021 gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc
     4081 tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt gatttgttca
     4141 atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac
     4201 aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt
     4261 ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt
     4321 cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag
     4381 gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt
     4441 cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga
     4501 ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg
     4561 cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc
     4621 ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa
     4681 tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac
     4741 cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga
     4801 ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt
     4861 tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct
     4921 gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca
     4981 tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta
     5041 aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat
     5101 ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca
     5161 acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc
     5221 cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa
     5281 caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac
     5341 cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta
     5401 cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct
     5461 tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga
     5521 ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt
     5581 caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta
     5641 attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa
     5701 ctccttttcc actatcggct tccttaatag ccgtctgaat ctgatctgga gttggagaag
     5761 taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg
     5821 atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct
     5881 tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc
     5941 cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc
     6001 catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata
     6061 ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa
     6121 cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta
     6181 ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg
     6241 attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc
     6301 tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta
     6361 tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta
     6421 acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg
     6481 gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc
     6541 gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga
     6601 tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc
     6661 gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag
     6721 aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg
     6781 taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg
     6841 gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg
     6901 gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc
     6961 ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca
     7021 cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc
     7081 tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag
     7141 gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct
     7201 aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg
     7261 tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct
     7321 gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca
     7381 agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt
     7441 gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg
     7501 tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt
     7561 tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct
     7621 tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt
     7681 tccgaggcac cgttttcagc accagaagag cttatggttg ttgaaggttc tgtttgagca
     7741 ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt
     7801 ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa
     7861 ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt
     7921 ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc
     7981 cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca
     8041 ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc
     8101 gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga
     8161 tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat
     8221 aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa
     8281 tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca
     8341 tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttctgt
     8401 gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc tcaggagcaa
     8461 aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct tctgtcgctc
     8521 gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc
     8581 cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga
     8641 tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca
     8701 tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc
     8761 taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt
     8821 ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa
     8881 ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg
     8941 ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat
     9001 actcgattgt atattcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga
     9061 acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg tataaaaaca
     9121 cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa
     9181 aaaaatacat aatagacaaa caagtatcta agaataaatt acctgtatgg gtgattgaga
     9241 tcatttcagt tccaccccat tttctttata caaatctgca atgaatgtcg tatctagatt
     9301 tttctcttcg gagacagaat aaaaacggac cgtttctctc tcttcttttg ttccaaaacg
     9361 ttccgtatac acttcttcaa gtaattcttg tctttttttc aatttttcat cttcatattc
     9421 aaaagattta tttacttttt tgtatagatt tcgattgttc cacctagtca atacaaatga
     9481 ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg attaagaaat acagcatttt
     9541 cttttctgat aaaaaagcgt acaaggaaaa atccttggct ctttctggtc tcgttaatgg
     9601 gaaaaagacc catataaaag gaagaacaac gatcatccat cctaaaagag ccactaacgt
     9661 ttgccaaatc tttaaccagt aattaccttt tgaaaaatac ttatctttca ccaaaattga
     9721 tgcaacttct tctcttgtta cttttttcat atcctcacct cacgtttttt tgttacgacg
     9781 taggagttta ccatacattt catttctttt cacataaagt gttcctaaat ttttcctaag
     9841 aaaaagaaca aaacatttct catcagcaaa tgaaaaatat tttgttcttt ttatcagttg
     9901 ttctttttat tttttatctc ttaattatgt tgctctttcc aatacgacaa tgcttttcat
     9961 tcctctagat atcgccgtgt acatttgtct gcgtcttctt tgctcatctc catcgttttc
    10021 aaatggatat aagattacat gatcaaattc cagtcctttt gctagactca aaggaatgat
    10081 ttggcagttt tctaatccat atgaattttt tttaatcgat tcttgtagcg tttgggtttc
    10141 ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt agagaaacca aaatctcagc
    10201 tagtattttc aacaattctt cagaggatcg aaaagagcgg tattcgggtt cttttccttg
    10261 cggtcggatc gatacgatgt cgatcttctc ttgatccaca gcataggttt tgaaaagctc
    10321 tgtgattgct ccgctagaac ggtaactatt tctaagatca taccgatgga tcggcagatg
    10381 gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg gaagaattaa agattgcctg
    10441 attctcgtca cctactaaag taaagtgagt ttttggaaat aattcgatca atagacctaa
    10501 ttgtgccagg gtataatctt gtacttcatc aatcaataga tagcgaaatg ctggtacatt
    10561 taccttttca acaagtaaat gacgaatagc taatataatg actgcttcat caacagtacg
    10621 tgtgggttgc caagcataag ctgagccata tcgtttttca taaattcttt caaacaactg
    10681 atgctcatcc acccatgcca tttcttcgat ttgtctggtt atttttcgat attttttttg
    10741 taaaagtttc cttgcataag ctacaatact ttgttccgaa tcatcagaaa tcaatttacc
    10801 gaataattcc aactgcctat cttctgtcaa cgagagtacc tgattctgca cagcaggact
    10861 ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc agtgcctttt tcgttgcttg
    10921 aagcctatcg atcaatttag gatgagcagg aactttctca tatatttttt cgatcgtttt
    10981 tttagaaagg actgtttttc catttttagt aagtccacgg ataaaattcg gatgatctaa
    11041 aaacaaagga tctgattgtt ttagattatc gataaacttt ttgctacgca aacgttctgt
    11101 ctgctccgat acattttctc ccgtgatacg tttgaaataa gcctcttcac cttctatttc
    11161 ctctgctgaa agctgtgaaa ccagctgtat gatggttaga ttcagtggat ttctttcacc
    11221 taatgatggt aggacattcg aaatatagtc gataaaccga tgattaggtg atagaatcag
    11281 caagtcatcg gatgtcatcg tatctcgata taaatagagc agatacgcaa tacgctgcat
    11341 gatagcagag gttttaccac ttcctgctac gccatttacc aagatatttt gacttttcac
    11401 atcccgaatg attgtgtttt gttccgattg gatcgtagca gtgatagcct tcatttcatt
    11461 ggtctcatct tggcctaaga cttctaataa tacgtcatct tgaatagcta cagacgtatc
    11521 aaaaaacttg accaaacgat ctctttccaa aagaaattgt cgtctttctt taataaccgt
    11581 atcgatttta ttttgacgaa ccgtataaga agatggacca atcatgttat tgtaaaaaag
    11641 ttcggcaatt ggtgaacgcc aatcataaac aagattttca tctgcatcat ctgtgaaatt
    11701 tgctgtaccg atataaaagg attcttttgg ctcattatcc agaaagtcta caacgacttt
    11761 tccaaagtag ggacttttca ataaacgttc gatctttgct agctgatttt ctgcgttttt
    11821 ctgtttgata ttcatctgat cgatttctct atttttcatt tcaatcatcg aaaaagtatc
    11881 caggttatct aagtaactgt caaaattcaa tcgaagctcg ccagccattt ctttcaacga
    11941 ctggtttcct tctatttttg ccgtatccat cgattcccgc agttcgtttt ttttatcaat
    12001 tagcttttga tacacgttct tcagatatgc ttcttctaac tgtttttgtg tgttgaccat
    12061 acacattctc ctcttttttt aaagtggtgg aaccgattag tttacctctt ttaagagtcg
    12121 aatgcaagct ttttaacgag tatcatttgt taggatatat cagttcttca ggtcggacag
    12181 tttgatataa agaaaatgca ccatcaaggt tttttacgtg gtagccagac tgcttcaaga
    12241 ttctttccgc tagatagcta cgtaatccgc tatgacagct gacgatatat tcttgttggc
    12301 tgtctaattc atttaatcgg tcccgaagtt cattcaaagg gatatgtttg gcttccttga
    12361 atttgccctt ttccaattct tctgctgttc gcacatctaa taaaattttt ccttttgcta
    12421 gttctgtcgg caattcatgc cattgtacgg tttcactaag cccttctgca atgttcattg
    12481 ctgcatatcc tagcatgttt actggatctt tggctgagcc aaatggcggt gcatacgtca
    12541 attctaattc cggtaaatcg aagatcgtca aatgcccttt gattgctgtt gctaaaatat
    12601 cgatccgttt atccacacct tttgccccaa caccttgtgc accataaatc tcgccagtct
    12661 ctggatggaa gatcaacttc agtaaaatat ctgttgcttc tggataatag cttgcatgat
    12721 cttttcctga tacatgaatg acttcaaaag acagttcgtt ttctttggct gttctttcac
    12781 ttaaaccggt ggatgcggca gtcattccaa acgttcgtat aattgctgtt ccaataccgc
    12841 cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg
    12901 ctggtgaagc aagagaaata agtgcatctt ttccagtgat ttgctgtttt acaacgatag
    12961 catcccccac cgcataaata tcaggaatat tcgtttgata acgttcgtct actaggatac
    13021 ccccgcgtat gcctaattcg attccagcgg tcactgctag ctggttatca ggacgaacac
    13081 caatggataa aataactaaa tcagcaggga tggtacgccc atcatccagt cgcaatcgtt
    13141 tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat cacttctact ccttgatgag
    13201 acaattcttt ttctataaaa gctgccattt cttcatccag tgttggaaga atatgtggtg
    13261 cttgttcgat caccatgact tctaaacctc ttctttttag gttttctgcc atttccaatc
    13321 cgatgaatcc tgcaccaacg atcacggcac gctttggctg tttttctaat gcatgcatca
    13381 ctttatcgat atctggtaca tttctaatag aaaaaacatt gtcggcttct gttatccctg
    13441 gaatcgatgg aacaactggt tttgcaccag gagataaaac caatgcatcg tactgttcaa
    13501 tgtgtttttg accctcatgt acgacttcta ccgttttgtt ttcgggaaag atggctgtca
    13561 cttcatgatt tggacgcaca tctaaacaaa aacgagcgga taatgattct ggcgtttgga
    13621 caagaagatt ttcccgttca ctgatttctc ccgaaagata ataaggcaag ccgcaatttg
    13681 caaaagaaac atacggtcct ttttcaaaaa caacgatttc tgcatcttcc attaatcggc
    13741 gaagccgtgt agcagcagac attccacctg ctacacctcc gacaataacg attttcatct
    13801 tatttccctc ctactgttcg atcaaaccat tgattcatgc caccacgtac attcactaca
    13861 tcataaccag atttctttaa ttctttagct gcttgtttac tcctcatccc tgattgacaa
    13921 atcacataga cggtcttttc tttgtctcca tcatatctgt ttatactttg taatggaaca
    13981 tttttagctc cttttatatg cccgccccgg tattctgcag gtgttctaac atcaagtaac
    14041 gctggatttt ccaaaagctt ttcctttaat tctgggactg ttatcgattt tattgattga
    14101 aatagcaaat tcattttctg cacctcattt acatttttat aaatacctca tggggtatat
    14161 taaacaggag ctttattaat atgtcaacca gaatgactct tcattttttt cactactttt
    14221 tatatttgca taaaataccg ataaggggta ttatctaatt ataaaaatag aggagaatgt
    14281 gttatggaga ttgataataa aaatatcctg aaccgtttga aacgtacaga gggacaaatt
    14341 cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat cactcaatta
    14401 agcgctgttc gttctagtat tgatcgcgta atgggcatga tcgttgcaga taatctgaaa
    14461 aattgctttg agaaacctga tagtaatcca gaagaacaag cggctaaact agaacaagcg
    14521 atcaagatga ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt tccatattga
    14581 tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa aacctgataa aagcttctcc
    14641 agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt accggttatc tagactctgc
    14701 caatgcagaa ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg
    14761 tgctaatcat atttcagaaa gttctttacc tgaacaagta atcgttgtac cagcaggtgt
    14821 ccttgattta gttgaactgc aatcccaagg ttatgcttat atcaagcctt agtcagtaga
    14881 ggttgcgaca gaagtgatca gcttcaagaa atttcggctt atttttcgaa gaagctactt
    14941 ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagta ataagaacag
    15001 ccaaacaaaa atagcttctc atatttttcg tttggttggg cttattaccg cagatgcaag
    15061 cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg gtttgaccta agtattagag
    15121 aaaaaaacgg gaaaatagct cctcatattt tgccgttttt tgagctaata ccgaaggtca
    15181 gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt cgcttagttt cgagtattaa
    15241 gaaaaatttt gtgaaaatgg atttctacat ttttgcataa atttggctta ataccgagaa
    15301 actggctttt gaataccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat
    15361 tagagaaaca aacggaaaaa tagctgctca tattttgccg ttttttgagc taataccgaa
    15421 ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctaccattta actccgaatc
    15481 acaagaaata atttcaaaaa caacttctcc tgtttttggt tcggttggct tattatcgag
    15541 aagtagctac tgggagtcta tttatcaaaa aaaggaatgc gacataggtc acaccccttt
    15601 tttattttct ttgaaacatc tttcggtact cgacagcata caagacaaaa gattgtaaaa
    15661 gaccgtaagt gatgacggca aaaatgactc tcacccacag cgttggtaat tctgggaaaa
    15721 gcagcaaaag gatcatgcca acatagatga cagcagtcgc tactttgcca taccacttcg
    15781 ctccacttaa tttgatattt ttagatagcc cgattaatcc agctacaaac ataaagcctt
    15841 cttttatcaa gaatagacca aacaacagcc aaagccacgg tcgataccat gccatagaca
    15901 aaatcagaaa aagttgtgtc aatttatctg caaacggatc aagcaccttc cctaagtctg
    15961 ttacctgatt atatctccga gcaatgaccc cgtctagata atcagacaaa cctgagacaa
    16021 caatgaccgc tcctgctata taaaatgact gtctcagcac aaaataaaga taaatcggca
    16081 gcaaaaaaat acggaatatc gataaaagat tcggaatcgt ttgtatttct ttacgccagt
    16141 ctgtgcttaa tttcatttta tcgccctacc tcctcagcac tcttttcttt ttattttagc
    16201 ttacacgggg aataaaacca atgatttcac tgtttttatt tttctttaca gacactgata
    16261 ttatgagcta atttgagata atcacttgct gttttcatcg tttactcttc tttcttcctg
    16321 tcgtttgcgc atttctttta ctagtacttt atgagctttt tttgatcctc gctctaggtc
    16381 acgctgccgt ttacgtgttt tataatcgat aaacagactg tctaaatcgt aacctgtcgg
    16441 gtacagatct tctgctgaac gcaccagacg aacacgctga cgaggcacgt ggattatttt
    16501 tttgtctaaa tatacatcaa tcgtatcttc acctatatct tcataaacta acgcttcttt
    16561 ttggtattca ttgacaaata tccgatcacc ttttgcaaag agctgacgtt cttttttatt
    16621 ctccttccta tttgtaactg ctgtcttagg aagaaattct tttttctgtg tccgataggc
    16681 tttttccgcc aagtaacgct ctgcttgatg gatcaatttc atcgacatat ccattttatg
    16741 agcaatccac aaagcttggc tttctcctgt ttcttccaac ccgcaactga tatttaggac
    16801 gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa atcttcatga tctccggcga
    16861 atttcttgat ttctccataa tgggtcgtgg caatgatcaa tgccccttgc tcatacatag
    16921 attccatgat tgcgatcgct agtgcagccc cttcatttgg ctctgttcca ctgccaatct
    16981 catctaaaag taccaacgta ttccttttta tctttttcaa aatagcagcg atgttttgca
    17041 tatgaccaga aaatgtactc aacgcatttt ctaaattttg gtggtcaccg acatcgacga
    17101 aaatctcatc aaagacagca agctctgtcc cttcttttgc cggaacttgc ataccgaaca
    17161 tcgccatcaa agtcaataaa ccgactgtct tcaacacgac tgttttgcct ccggcatttg
    17221 ctccggtgat aatcaagcct cgatagtctt taccgatttc aacatccaat ggtaccgcat
    17281 gctcaggtaa aaatggatgt ctgccttgct tgataatgat atgttccgat ttattgattc
    17341 taggtgtgct tccattgatt tctcggctaa attttgcccg agcaaagatg atatctaaca
    17401 ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata gcaccagtca
    17461 atgccgccag gacttgatat tcttcactga tttcttcagc ttttaacaac tgatagtgtt
    17521 cattcaattt agaaatcaca gttggttcaa taaaaaccgt cgttctttta tttgattcat
    17581 cgataatcgt tccatctatt ttatttttat agctggcttt gataggaatc gtatagtatt
    17641 cgcctttttg aacaatcatt gcttcttgga tcatttcttt gtttttcgga tgacgcaaga
    17701 atttcagtaa ttttgactgg atttcttttt cgattagttg taattgtttg cgtactttac
    17761 gcaaattacg tgacgcgtcg tcactgactt tattattttt tattttctgg tcaatcaact
    17821 cttctacatt tatcaaatca ggaagatgtt tgctataagc aaacaacaag ggtgtctggt
    17881 attgattctt atcaaaaaac tttgtgatca tccgactgct tctaagaaaa tccgcatatt
    17941 cgatcaaatc ggttggctgc agaatcaacc cttttttgac ttgttctgtc agtgcatcga
    18001 tcctcggcaa ccccataaat ggaacatgct gactgctttc taagatcaag cgtgcttctc
    18061 ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc atatcgctga
    18121 ttcgtttttt gctatagttt ccaattgctc ttgcttggat ttcctttttg atccgatcaa
    18181 attgtaattg actaatcatt tcgttgtcca tattctctcc ttttcttgaa gagatacctg
    18241 tcccaaaata aataagggcc tagagataaa taaaaggata gaacacagct taaaaaagtc
    18301 atgtcctatc cttattagaa tacaaagata ttgttttcct gacaggtacc gtttcataag
    18361 cacaccaaat aaactttgtt tattggctat tcgacttact gattagatac cagctacact
    18421 cacaaaacat accccattct tctaggtctt atttattttc cacttaacta tagcaagaca
    18481 cattttggtt gtcaaacttt agtatgtttt atagatttac accattaatg aatagacacc
    18541 taccgcgata attgctccga tcattggacc tgcgattggt acccatgaat atgcccaatc
    18601 tgaagttcct ttattagcta ttggcaatac cgcatgtgct aatcgaggac caaagtcccg
    18661 agcagggttg attgcatatc ctgttggccc tcctaatgaa agaccgatgg ctaagatcaa
    18721 tccacctaca gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa aagctagcaa
    18781 cccaagaact aatacaaatg taccaattaa ttctgtgatc acatttgctg gataattccg
    18841 tatagcagga cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg ttgcattcca
    18901 atgtggcaaa taagaaagcc aaacaagaat ggcccctgca aaagctccta aaacttgcgc
    18961 aatgatgaaa ggaacgacaa gattccaact aattgcccct gtcatcgcca tagctagaga
    19021 gactgcagga ttcaaatgag ctgggcccat gaatccagaa atatagacag ccattgttac
    19081 agcgagtccc caaccaaatg cgatgacaat ccagccagaa gcctgtgctt tacttttgtt
    19141 taaattcaca gccgcacaca caccatctcc aagcaaaact aggatcagtg tcccaataaa
    19201 ctcccccatg atttgagtca tatcactgtt catttatttc tcccccttta gttcccgcag
    19261 atctgattct gcaatcaatg cttctaaacg tttagtctgt tgcgccttct cctcttctgt
    19321 ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc ttgatctcat caagctgatc
    19381 tcgttcaaac aacagatgat ttgtacgacg aatgagataa tcgcctgggg ccaaaaccat
    19441 ttcttcttct aaaccataac gtaaccgagc tgactcagcg agactcaaac caggataagg
    19501 tgccatttct tttgctaatt caaagatacg tcgggcatta gtcccgtaaa aatcagcgat
    19561 ataagtagca tcttcttcct ctaaaccggc tgctactcct tcttttgcta attctgtcac
    19621 tgtttcttct aatttagttg gatcgaagtt tccacctgaa atctgatatt ttttagagtc
    19681 gatttctttc gtctctattc cgtattcttc ttttaataat tggcgtatca atcgcaaagc
    19741 tccttctgcc atcttacggt aatcggtgat tttcccacca gataaagtca gcaaaccatc
    19801 tggttctctt tctaaagaac tacctctgga aatcgtagaa ggtgcttttt catcacggct
    19861 gttttccaaa tggttcaaca cgtcttctac ttcagcacga gaaactttgt tttccttata
    19921 ttcacttact gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc cattatctcc
    19981 accattgtaa tcagaaccag aattaccaat caacagtgga cgaagtcctg cccagcttgc
    20041 ttcgatatca ttcaatgtga tatttgcttc tggatagcga tgattgatca catccaatag
    20101 ataatctaca tcttcttgtg tgactttcgg atccgtaaaa tctccttggt aatccgtatc
    20161 tgtcgtacca aagtaagtct tgttttctct tggaatagca aaaaccatcc gcccatcttg
    20221 ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat ctacaactaa
    20281 atggatccct ttagttggac gcattttagg agagactgga cgcgtaaaat tcaagtttcg
    20341 tactttatct acccaaggac cactcgtatt gatcactaat tttgctttga tttcgatcac
    20401 ttcatctgtc agcaggtcac gagctttgac gccaacgatt tgatcgccat catataaaaa
    20461 gccaaccgct ttcattttac ttactagata agccccatct tcggcagcct ttttgatatt
    20521 atcaatcact aaacgggcat cattgttgcg gaaatctaga tagacacccg cgccttttag
    20581 cccttctttt ttcaaaaacg gttctctttc caatacttct tctggtgtca gcgtataatt
    20641 ctcatatttg gttcctgtca catttgccag tttgtcataa aggtccattg ctactttgac
    20701 cgagaacata ttgaaggttg ttgctccttc gccctcatag attggcaaaa gcattggatc
    20761 tggttttggg atgtgtgggg caatactttg tacgactgca cgttcaccaa ctgtgtcagc
    20821 tactacttcc acatcaaatg tcttcagata acgaataccg ccatgaacaa gtttggtcga
    20881 gcgagaggaa gtcccttctg caaaatcctg catttcgatc aatcctgttt tgattcctga
    20941 tgctgctgac tggactgcca caccggcacc agtgattccg ccaccgatga tcaacagatc
    21001 caattcttct tgctgcattt tttgaatgct atcttgtctt gtcttgtttg aaaacatatg
    21061 gatttcctcc ttattcaaat gcttgggtag cttttactgc ttttttccaa ccgttgtaaa
    21121 gtttttctcg gcgctcttcc tccatttgaa cttcaaatag cttgccagct tcataaaatt
    21181 cgcggatctc gtctgtatct ttccaataac cgacagctaa tcctgctaaa aatgctgccc
    21241 ctagcgccgt tgtttccaag ttttctgctc gttggatcgg tacattcaag atatctgttt
    21301 ggaattgcat cagatattca ttgtttgctg caccgccatc tactttcaat acaggaatct
    21361 tgattccagt gtcatcttgc atcgtatcga tgatatctct cacttgataa gcaatcgact
    21421 gtaaagtagc tttaatgata tcttcttttg tcgtaccacg tgtcaagcca aacatagccc
    21481 ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac gaatgcagga acaacataaa
    21541 cctcatcttc gcttgtagag cgtttcgctg cgtcttctga atcgctagct ttttgcaaca
    21601 tttgaagtcc atctctcagc cattgaatcg aagaaccagc cacaaaaata cttccttcta
    21661 aagcatagta aacctttcca tttatgccgt aaccaattgt cgtcaataaa ttatttttgg
    21721 ataattgcgg tttttcaccg gtattcatga caataaatga acctgtacca taagtattct
    21781 tgaccatgcc tggttcgaat gccatttgtc cgaataatgc ggcttgctgg tctcctgcca
    21841 tccctgaaat tggtgcttcg cttccataaa aatgataacc ttgcgtataa ccataaactt
    21901 ctgagttaga agtgactttt ggtaacatca ctcgaggaat atttagtaaa tctaaaatct
    21961 cctgatccca atctagctga tggatattga acaacatcgt ccgactggca ttcgagtaat
    22021 cagtaacatg tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg atcgttccaa
    22081 acatcaattc gcctttttca gctcgttctt gcgcgccttc tacatgatca agaatccagc
    22141 gtatcttcgt tgctgagaaa taggcatcga tcactaaccc tgttttttcg tggaaaaatt
    22201 cttgatagcc ttcatttttt aagttatctg caattccagc tgactgtctg gattgccaaa
    22261 caactgcatg atagattgga cgtcctgtat ctttttccca tattactgtt gtttcacgct
    22321 ggttcgttat gccaatccca gcaatctgat ttggtttgat ccctgattcg atgaacgctc
    22381 cggcaataac tgactgtaca gaattccaga tttcatttgc atcatgttcc acccaacttt
    22441 cttgcgggaa atattgcgtg aactcttttt gagaactgcc gatgtgtctt cctttcttgt
    22501 caaagataat tgctcgtgag ctagttgtac cttggtcaat cgccatgata tacgttgatt
    22561 ctgtcattgt gattccctcc attatatgca aacgctttct ttaactactc ttatcatact
    22621 actgttttaa aaaaagaaac tgtcaaaatt ttattctttt tttctttttt tgacagtttt
    22681 ttattatgaa gctatttttt aatcaattct tttctttttt cagcgtctct attcttcttt
    22741 tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag ataatattca cactgttttt
    22801 tgtttctctg gcaaaatgtg atcaccaaat catattcttt accttcttca taacttccaa
    22861 tctcaatacc gccgattccc ctcagttctc tgatcagata ttgttgaaaa gcaatacgat
    22921 aaatcggcag actttctaaa tcgattccaa tcactactgg ttttgcaata taaaaatcga
    22981 tcatcaaaag aacattggca tactggatca tcaggtaact aagctcacta tcttcagctc
    23041 ttttcctcgg caattgctgc accgctgttt cctgtaattt cgccagtaac tgtgttaagg
    23101 aatgcccgag cattttctgc tgctgttcca aaagccgatc acgctcatag acttcgatct
    23161 ttccaacaaa gaaaaaccat tcattgttga tctgggaaat ttggtagcct actgcctttt
    23221 cttctttgat tttcaaacga cgaggacgat aatgccataa catcgtttcc cgaatgtaag
    23281 tatctaaaac agcggttggt aactttttgc tccgtgtcaa atcatactga taaaatattt
    23341 cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat
    23401 tcagttctgc tgctgttcga ctaagatgca tcacgatgat tggatcgatt gcttgataaa
    23461 gccggtccga ctgatagagt aatttctttt ctttcaaagt tgcgtacgta cttttttcat
    23521 tcaatagtct ttttttcgta atcccgagcc agcaagcaat ctgtgcttct gctgatgccg
    23581 taaacgttgt ttctaataca cggtccaatc ctagaatgaa cggtcttttt tctggtgtat
    23641 tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg gaatagttca taataaaaat
    23701 atcgtatctg tagctcttct ccgtataatt gaccgttttt gatttgtaat ccaaattcct
    23761 ctaagagctg gttcaattct ttgatctttc gaaaaacaga agactcactg atattaaacg
    23821 ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa taaatagacg agcatcttaa
    23881 acttcaagct ttcctgcaat aaaaacaata gaattttttc caaacttaca tcttcgtcca
    23941 acacaagcag cgcctgatac tcatttcgct gtacttctac ttttttttcc atcattcggc
    24001 caatcaattc aatatcttcc aaatattgat caaacgaggc ttttgacagt tggacatgct
    24061 ctctcaaacc attgatagag gctgttcccc cttgcagaac aaggtttcgc aaaatcagga
    24121 tctgtaactg ctcttttctt tctaataatg cttcaatctt cataggcagt attcgccacc
    24181 tttgaccaca ccatatctgg cacacggtat tctggctgga aaaatagctc atgttcttct
    24241 aactgttcaa acgtccatcc ttcttctact gcaattgtca gttggtcaag aaggtggaac
    24301 atctcttttt tgctttggag ctgtgctcca agaatctttt tttcagaatc aaatagaaat
    24361 ttgagtttaa ctttgctttg atttattgct gtaagcttgt agtctacgta tcctgtttca
    24421 attttttggg gataaaaaat gctctcttct tctgtcaaac ctacgcttcc taagaaatag
    24481 ccaaaccact gattgccgac tggacgatag gttccctgat cctttgtctg tgagccacta
    24541 attgtcttgc tgacatttcg tgctgtacgg acagcgttat ttactagaga aacatacatt
    24601 ttttcatttg ttggacgaaa agttacttgg atcgcatcgc caatcgcata aacgtctttt
    24661 tgagacgttt gcaagtagtc atctacccag atcgttccat catcattcaa tgttaattgt
    24721 tcttcccaca tctgattgtc gggacgagaa tgattagcca gcaaaacaag atcatagttc
    24781 tcctttcttt tttccgtctc tacaatagcg gtttcatttt cggtcaaaga cacaacttgt
    24841 tcttctaaaa aaagtgaaag cccttgattt ttcatttcct ttactaacgg ttctgtcatt
    24901 tcaggatcta gataacgaaa aagaattgac tttcggcttt cgtaaagatg gatttgtttt
    24961 tttcttgatg ccaacccttc agctatttcg attcctgctt gtcctgcacc aataattgca
    25021 attttctttg cattcatgat cttttgctga agagccgtta cgttttgtat ctttttaact
    25081 gggtgaataa gcgttgattc tgcagaaata tttcgaaact tttgatttga accagtcgct
    25141 agaatcaaac gatcaaaatc caattttttt cctcctgcta aaacgacttt tttttctttc
    25201 aatcctatta ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt cagttcttct
    25261 ttcgtgatcc aagaaagttg tttttcactg gtaacctttc cttttaatat caaattgaac
    25321 ccgctgggaa tccatcctaa cttcgattgc ttttcaatca aatatatttc acttgttggc
    25381 atcaattggc gaagataaat agccgcatga atccctgcaa acgacccgcc gacaattaca
    25441 atgttcatct gcattcctct ttcttataag gtgttctact gctaacttct caatcagaaa
    25501 acacagtatt cataactcgc tttcaacctt atgatacata gcgatgcagc agaatcaacc
    25561 ctctccatca taccgtgttc taggtctgct tctcaataat gtactagtgg acctaattga
    25621 agagtatcat ttctgaagag atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg
    25681 ccttttacgt acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg agttttagcg
    25741 tgtgtaatgt aatagttaga atagacacat catttatttt taattcttta atgcttcaac
    25801 aactgcttcg cctaattgat gagcagattg aggattttgc cctgtaagaa gccgcccatc
    25861 aatttgaacc acttctgaaa aagctgctcc agactgatag tttgctcctt tttctttcaa
    25921 agcatcttct gctaagaatg gtacttcatt cgttgtacca tttgcttcct cttcttcatt
    25981 actgaaccca gtcactgtac gtccagctaa aatagatttt ccagactcat ctgtaactgg
    26041 caacaatcct acaacaccgt gacaaacagc tgagaccagc ccttcatttc gatagatttg
    26101 tgaagcgact ttcccaagat ctattgcttc tggaaaatcc cacattgttc cgtggccacc
    26161 agcataataa accgctgcat aatcatttgc atccacatca tcaggacgta aggtatttcc
    26221 caaggcccga tttctgaaat cttcatcttc ataataggcc caatcgattt catccatatt
    26281 caaaacactt tgcggttcta aaggtacata tccgccttta ggactgacaa aatctgcatc
    26341 gatcccattt tttactaata catcataaaa atgagtcagt tcacttaacc acaaacctgt
    26401 aggacgctcg atcttttcgt acttttctac atttgttaat acgatcaata ctttcatttt
    26461 cccatcctct ttctataaaa attgcgttat tgataacgtg ctttcaagaa attatataat
    26521 gggcttggca aataaagctc tttatatgct ttttcaaaag gtttcgttac aaattcatcc
    26581 tcacggtttt ccaataattt ctctgcccag tcaggatcga cgatcatttg ctgtccaatt
    26641 gctgcaagtt ctgcatattt caatacctgc tctacatctt cgcgattgcg gatcccaccg
    26701 acaccaacta aagggatctt tccttgaatc gtgttatgga ccacttcaag aatcgattta
    26761 tcgttgtatt tatcagaacg agctattcga tcatacatat ttaatgaaac atgcagataa
    26821 tctagttttg actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa acggatacct
    26881 ggtgtttcat attcttctgg tgagaaacga taccctacga tgaatggttt tttagcatat
    26941 tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa taaaacgata acgattttcc
    27001 aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc
    27061 aaatacgtat ttgctccatg caactcgatt ccatcaaatc ctgattcaat cgctctacgt
    27121 gtggctttgc caaatgcttg gattgtttca acgatttctt cttcactcat tgctcgtggt
    27181 gtttccgcat caggacgttt tgctgcaact gcacttgcag atacaggctg ttctccagat
    27241 aaagttgctc gacttgtcat tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct
    27301 tctccttgga ttgctttcgc tagctcactt aatcgaggaa tcatttcatc atgagcgatg
    27361 ctgagttcac ctggccagcc ttttccgata tcttggacat ttgcagcacc agtaataaca
    27421 gcaccaagac ctttagaacg ttgtttataa taagtgatct catcttcggt gatcactcca
    27481 ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca atcggttctt caattgaact
    27541 cctgaaggta aagtaattga ttgatcaaat tgtgacattg tcattcctcc cataattcgc
    27601 tctgattaac ctatcgagaa tcatctatca ttaaaccact cgattagttg tacatgcaac
    27661 taatttgctt aaaaaatttt attttttttg taacttatct gttgcatcat tcattgtctg
    27721 ggctaattct ttacttgctt catttcccaa aactgctttg tatgactgat gaagttcttc
    27781 ccagctacca tcgacttcct tagcaaattg gcgtccttgt tctgtaagat aaactaatga
    27841 caagcgacct tcgctaactg ttttgacgta tcctttatat tctaattttt caacaaaacg
    27901 agcaatcgtt gatggggcgg aatagagcat ttgtgcaata tctttttgtg gtattccatc
    27961 tttttctttt aatatcaata aaataaaacc ataagttggt gccagacctg tccttctaaa
    28021 cgcttcatca gctaatttat tcaacatgcg atctagcttt ttcacgctaa agaaaagaca
    28081 ttcatctaac gctttttcaa ttggcttttc catttcatcc cctccattgg cgtacatatc
    28141 atagtatgat ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag
    28201 atttgaaaaa aagaagtagc catcttcaaa gaatttcggc ttgttttcga caaaactcct
    28261 tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa
    28321 attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc gaaaaactgg
    28381 cttttgaaca ccgtttatat cagaggttgt gaaaaagccg tcctgcatca agtaataaga
    28441 acagccaaac aaaaatagtt cctcatattt tgtcgttttt tgagctaatg ccgatggtca
    28501 gctgattgta caccgtttat tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag
    28561 gagcaatttc aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt gatcgaggag
    28621 ttggcaggag aatactgttt attcgtaaag aaggggctgt gacaagactt ttgtcacagt
    28681 cccttatttc cttttattat aggatttcgc catttgattg gatgacttgt ctgtaccaat
    28741 caaatgattt tttctttaac cgtttctttg tgccattccc ttcgtcatct tgatcaacat
    28801 agatatatcc ataacgtttt gacatttgtg aagtagaagc gctgacgata tcaatgcagc
    28861 cccacgacgt ataaccaaat aaatctactc catctttgat agcttccttc atctgctcaa
    28921 tatgctgctt caaatagtca atgcggtaat cgtcttgaac ggttccatct tcttctactt
    28981 tgtctatagc tccgagtccg ttttcagcta tgaacagtgg tttttgatac ctatcatata
    29041 attgattcaa agcaattctc aaacccttag gatcaatctg ccagccccat tcacttgatg
    29101 gaagatacgg atttttaatt cctccagtta ttaagtttcc ttggacccct tcccaatttt
    29161 ctggatgagc actgactgca ctagacatgt agtagctaaa agaaacaaaa tcaacagggt
    29221 aggctgctaa gatttcttca tcacctactt ctatttttaa gttgacacct ttttcttcaa
    29281 acattcgttg cgtatacgct ggataatatc ctcttgcttg aacatcacta aagaacaaag
    29341 aagttctttg ctgctcaatt gttttttgaa tatcatccgg atcacaagaa tacggaacaa
    29401 gaagctgata gctgatcata caaccgactt gtgaaccttt gataatttca tgacaagctt
    29461 ttgtagctaa agcacttgct ataaactgat ggtgtgcacc ttggtatttc gcttgttcca
    29521 aatggggatg gttttcttca attactccta cgctaacaaa agggtgatgt tttacacagt
    29581 tgatctcatt gaaagtcatc caatatttta ctttattttt atagcgtttg aatactgttt
    29641 cagcgaaacg aacgtaaaac tgaataagct ttcgattagg ccatccacca tattctgtaa
    29701 ccaaatataa cggcatttca taatgggaaa gagaaacgac tggctcaatc ccatatttta
    29761 gacattcgtc aaacacttga tcataaaaaa ataatcccgc ttcattgggt gtttcttcat
    29821 caccatgagg aaaaattctt gtccatgcga tagacatccg atagcattta aaacccattt
    29881 cagcaaataa acggatgtct tctttaaagt gatgatagaa atcatttccg tgtcttttag
    29941 gataataata cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat
    30001 gccattgatc tgtccatttt tctttaggta aatcgctgtc gaatgtatac acatcagaca
    30061 cagataatcc tttttcatct acatcccatg caccttcaca ttgattggcc gctgtcgctc
    30121 cgccccataa aaatccttct ggaaattttt tcatatcgat ccccctaaac ttgctagttt
    30181 tcttgtttga ttatttttct tatgactagt aatgatattt ttgaatctac tactatttgt
    30241 aacaataacc attgtggtcg gatcatatcc ctctttgatt aattcttgca aatctacttc
    30301 catcaataaa tcgcctttac gtactttttt tccatcagat actgcacttt gaaagtattt
    30361 gccatttaaa ttcactgtat caataccgat atggatcagt atctctgctc cttcttcact
    30421 gactacccca attgcatgtt tcgttggaaa aactgttgaa acttccccgt ttacagaact
    30481 gtagattttt ccgtccgttg gaatgatcgc aaacccttct cctaaagatc cactagcaaa
    30541 tgttggatcg tttacttcag ataaatcaat tatttcacct gctgcaggag tacttaacac
    30601 tatatccttt gaggattctt cttcgatttc atccttgtag ccgaaaagaa aagttagcat
    30661 acaggaactg atcatcgcta agaaataact agcaactagc caaacaaatc cttcaccaat
    30721 ataaatcggg aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc
    30781 accagctact ccaccaaata tagctccaca tatacaagca atgtaaaatg gtcgtttata
    30841 tttaagagtc acaccataaa cagcaggttc agtgattcca aataatgctg tgataaatgc
    30901 agataaagat aaacttttta ctttcttatt tttcgttttc aaaaataccc caagtactgc
    30961 tcctgcttgc gcagctacgg cagtcataca tgctggacct agcgtatcac gaccgtagaa
    31021 agaaagattg ttggtcatta ctggtacaag tccccaatgt aacccaaaaa tcactaacac
    31081 ttgccaaaat ccgccgataa cagcaccaga taaaacaggg ctaaagttat aaacagctaa
    31141 atatccagct gcaatcagct gacttgcata atctgcaatt ggacctacaa ctaataaaat
    31201 aattgggacc atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg
    31261 caagcttttg cgaatcttag gctcaatata agaaagaacc catacagcta aaattgctgg
    31321 aataaccgta cttgtatacc ttgctagaat cacgggcatc cctagaaatc gcaaggttat
    31381 gctatcacta tatgcactga caatcgtagg ataaacgaga gctgctgcaa taaccatagc
    31441 gatgaaacga tctgtattga acttttttgc agcagtatat gccaaaaata taggcaagaa
    31501 atagaaaaat gcatcagctg ctgcattcaa aatgatataa gttcctgatt cagtagttaa
    31561 taaattcaaa ctcgtacata aggcgagtac tcctttgatc aaaccacttc ctgccaagac
    31621 gcctaaagtc ggggcaaaaa cagcggcgat catgtctata aaagagttta aaatgccttt
    31681 tttctcagtt cctctctttc cactagatgc atcatcttct agtttcattt gtttggataa
    31741 tacgtcatac acttcattta ctgcatttcc tactacgact tgaaacattc ctccagcttc
    31801 gactacagtg atcacacctt ctagttgctc taccttctcc ttatctgcct tcttattatc
    31861 ttttagggta aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc
    31921 tccgacaaat cggacaatat ctttcgctaa ttgtgaatag tccattttta cttccccctt
    31981 gttaattaat gccgtagcaa tccttcttac aagataagta tagttcttag aaaacggttt
    32041 ctccattcaa ttagtacccc ttctaatagc gaacgaaact gtacaatagt gtgtatggtt
    32101 tacttagccg agcaaatcac tcactgcttg aaagaaaaca agtatatcgt ttctcttata
    32161 gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa gtggtatctc
    32221 aagctgatac tctgtgattt ttgtttctac ttggcttttg ataagacagc acaacaaagt
    32281 ctcggcattt aacggaagca taactaggtc agaactagga gagattagtt gctgaatgga
    32341 atctatcttt ttatggattt ttatttgatt ttcttctttg tagtaatgat caagcagttt
    32401 tgttttcttt ttttcttcaa ctatggtcag cttttcacgc aaaatctgat aaatctccca
    32461 agattcaaac tcatctagaa cactaggtag ataaatcaaa tcatcccact caaaaataag
    32521 ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt gatttttgaa
    32581 aagatacact ggttctatcc ggcattcttt gatccacatg ttcatacttt gttgaatctg
    32641 tttttttagt gtattcaaaa tactaattgg ctggttacta acaatcagta tctccccttg
    32701 ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca aaataagcgc tttcagctaa
    32761 attgggacgt aattgaaaat gtattgggaa gaaacgccga attaaataaa tttctaaagg
    32821 ataacgggag atgatctttt gtacaaaatg attcatggta ttatgtccgg catccatcct
    32881 cgttttcatt tgtttgatat gggttagcaa aaattctgtt tcatggaaca ataacgaaac
    32941 aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct cttagattct tctctatttc
    33001 tttgtttttt tcatcattaa tcatcaaata atttaactct aatagtcgat tttcaattgc
    33061 ttgtttttct tgattggaaa aagaataggg tagttctgat gataactttt ttgtgagggt
    33121 ctctaccatc ggaataaata atgtatacct gtttgttttt tctaaaagtt cttctcttcg
    33181 aagaatggtc aaaacgagat accgagaaaa tcgtatgaag tcctctcctg aaacaatata
    33241 attatatgat ataagaacct cttttaatgt cttttggaca gtcggcatga tattggaaaa
    33301 aattgtactg atttgttgag gaaaccttgc gtgttggatc accgtttctg ttccaacaag
    33361 agataagaaa attctttttg aattttcttc agcaatgatt tttagtcctt ttgtcgacga
    33421 aacttccagt tctaatttag gattgcgctc gatccaacgc aaaatcgttt ttatttgtaa
    33481 tgatacggct gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac tgacataccc
    33541 tttttgaaac aataagtacg tcacaatagt tgaagctcgg aaatccatgc tggaatagat
    33601 tttatgtttc gtcacctctt gtatgatttg ctgtttagtt tcagattcaa ttgattctaa
    33661 aaaataacct gaacgtttat ttgactggat aatgattttc ttctcgtaga tagcaagcat
    33721 atttttgatt tcagtttgga tcgttttctt gttggtacct aaagcttcag ctaattcttc
    33781 gcttgttatc caatgattct tatttcttaa tactacaaga atttggagtt gtcgtttatt
    33841 taagtccatt tgcttacacc tcctctttgt tataaacata caggaaaaac ctctcaaatg
    33901 acaatagtca cttgcagagg tttaaaaatc atttgaagaa agatctatat tcaaggtttt
    33961 taccattaga ctggataatt tgtttataat attcaaaact atcttttttc aatctcctaa
    34021 agcttccgct cccatcattt tgtctatcca cataaataaa gccatatcgc ttcctcattt
    34081 cccctgttcc tgccgatacg atatcgattg gtccccacca taagtaacca atgatttggc
    34141 aaccatcttt tattgcctca gcaatttcct gaagatgtat ttttagatac tcacaacgtt
    34201 ttggatcgtg gattcgattc aattcatctg gtgcttcatc tagtccaatg ccattttcaa
    34261 ctatgaataa tggtaattca taacggtctg tcaattcatt catgacataa cgaaacccta
    34321 gaggatcaat tggccaactc caaggttcgg gagatttttc cttcaaaaag ggattatcga
    34381 aaccatttgc tccgccagta tctacgcgca tttctgcttc ttttttatag acagcgctac
    34441 gataataact aaatgcaata taatctgccg tgttagcttg caagcactgt tcgtctccat
    34501 ctaacattac tggtgcacag tcttcttcct tccatgtcct gtaaatataa gctggataat
    34561 gccctcggca catgacatcg gagaaaaacc atgaattgcg tttaaattgc agtgaaccaa
    34621 atacattctc tggatcacag tcatatgcat aagtcgctaa tgcagaaaag ctacacataa
    34681 tacccatctt agcatttgga tcgattttct ttaataattg agtagcacgg gcgttcgcta
    34741 gaaacatatg atgacaggct tgataaatca ctttttttgt tagtgtagca atgggttcaa
    34801 gtttatctct agcagggaat gtaaccaatc cagcagctgc ataaggcata cgaaaagcat
    34861 tattgatctc attaaaggtc atccaatatt tcactttccc ttggtagcgt tcaaagacgg
    34921 tctgcgcata aaattcaaaa aagtcgatta attttcgatt ttgccacccc ccatactctc
    34981 ctaccaatgc taagggggtt tcataatgac taagagtaat tactggctcg atatcatatc
    35041 gatttaatgt attgattagt ctgtcataaa atacgagacc agcctcattt ggcttctgtt
    35101 cgtctcctct tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct tttaaaccta
    35161 actccttcag taaacgaata tcctcttcaa atcgatgata aaaatcaatc gcttcatgag
    35221 aaagataatg gagtgattcg tctaaagcaa tctcccaaat ctttttttct tcattccact
    35281 gaatactagg atgctggttc atgatcccga taagcgtatc tgtcacatta ggtaatttgc
    35341 cgtcttccaa ccatgcacct tcacattgat tagcagctac tgctccgccc cataaaaaat
    35401 tttctggaaa tatttcctga tccataacta tctctcctcg ctatcaacaa tttcttactt
    35461 gaagtatagc tcgattcatc aaaacaagat attctaacta tttcggcaga agagaagaaa
    35521 aaaattgaat gctactcttc atttttaata tttatatata acaaatacat aagacgatcc
    35581 ccgaaagaaa acactcgtta accatctaag caaacgattt attttacttc caacacaaat
    35641 gcttcataag gtcgtaaatc aactttgccg tctggtaact gtttcagagg ataatttgac
    35701 acaataaccc ttaaagcttc acgtggaata gtgaacgtct gcttttcact ggacatatta
    35761 caaataacga gccattgctg gtcttcatat atgcgagaat aggcaaagac atgctcaggc
    35821 gtcttttcca ctaattcata gtctccccag acgatgatgt catgttttct acgcaaatcg
    35881 atcaatttct tgtacatata aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg
    35941 atttctttat aatttggatt gactcgaagc catggaattc cagtagtaaa cccagcatgt
    36001 tcacgggcgt tccattgcat aggcgtccta gcattgtctc ttcctttcgc cttgattgat
    36061 tccaagattt cctcttttgt aaatccagaa gaaatccttt cgttatacat attgattgtc
    36121 tcaatatctt ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc taactcttct
    36181 ccttgataga tataaggggt gcctttcatc atatggagca agatagcaaa catcttggca
    36241 ctttcttttc gatacacatc gtcatttccc caacgagaaa caattcgcgg aagatcgtga
    36301 ttattccaaa ataaactatt ccatccttca tcacctaaag atgtttgcca tttggataat
    36361 acttttttca gttcagagat actcaagggt ttcaaatccc atttgtcttt gccttcttgc
    36421 tgatctaatc ctatgtgttc gaattggaag accatggata attcatttct tttaggattg
    36481 gaataaagtt tagcgatttc ggtagttgct ccccatgttt cgccaacagt catcagattt
    36541 ttgtcaccaa aagttgcttg attcatttcc tgaagatact catgcagttt aggtccatta
    36601 cttgtgattt ctttatccgg ttctttgcca atcatatcga tcacgtccat acgaaagccc
    36661 ccaatacctt tatcgatcca gaaattcatc atttgataaa tctcttggcg aacctgttta
    36721 tttttccaat tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt
    36781 ttttcatcca actgccaagc agaaccactg aatattgagc gcaatctatt aggctctttt
    36841 ccattgaccg gatcacgcca aatataatag tcccgataag gattttcttt atttttacgg
    36901 gcctcaatga accacggatg ttcatcagaa gtatgattca ccaccagatc catgatgata
    36961 cggatatttc ttttctttgc ttcttcaatc agttcttcca tttcttccat cgtaccgaat
    37021 tctgccatga ttgcttgata attactgata tcatacccat tgtcatcatt cggagactcg
    37081 tatactggag aaagccaaat tgcatcaata cctaaatacg ccagataatc caacctggaa
    37141 ataatccccc gcaaatctcc aataccatct tgatttgaat cttgaaaact tcttggatag
    37201 atttgataaa caactgaatg gtgccaccat ttttgttcca tgattttcct cctataaatg
    37261 gatgtttcta ttttttgctt aatcatttat tttagtctct ctcactaaaa attgtaacgc
    37321 tttcaaatag ctattgcacc ttgttatcgg taacagaata aaacaaaaaa acagctgagg
    37381 aaattccctc aactgttttt tgaaaggatt gatttatgca gggaagaatg ctgatttcac
    37441 aacttcttta taaacggtgt tcactcagct gtcccttggt attagctcaa aaaacggcaa
    37501 aatataagaa actatttttt cgtttttttc tctaatactc aggccagaac gctgatttca
    37561 caacctcttt atttcacatt caatatccac atgaatccca caaatacaaa gaacagacca
    37621 tacatgattg gagatacttc ttttcctctt ttagctgcta acatggtaat tggatagaag
    37681 ataaaaccta atgcaatccc atctgaaata ctataagtca aaggcatgcc gatcaagatc
    37741 aagaatgaag ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa agattgtgcc
    37801 atcaatactc ctaccataat caatgctggt gcagttactt gtgaagtaac aactgatagt
    37861 aatggagaaa agaacaagcc taacaagaag aaaatccctg tagtaatagc tgtgatacct
    37921 gaacgtcctc caacggcgat accagctgat gattctacaa atgcaccaac aggagaagta
    37981 ccaaataagg aacccgcaag catagctgtt gaatcagaag ctaaagcttt ccctacacga
    38041 ggcatcttgt tgtctttcat gaaacctgct tggtttgcta atccaactaa agttcctgct
    38101 gtatcaaaga aagtcacaag caagaatgtc agcactacga cccacatttg aagagaattg
    38161 atgtccccta catgttttaa tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca
    38221 acaatttcag aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc
    38281 atcccgatga agatgccacc tggtacacgc cgaacaagca agatagcagt tacgactaat
    38341 ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac taatgtagat
    38401 tcattcgaaa cgatgatccc accttcgctc agaccaagga aagcgataaa tagaccgata
    38461 ccgccagaaa tcgcatattt caaatctgct gggatcgcat caatgatcat ttcccgcagc
    38521 ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc taatgctgtt
    38581 tcccatggaa tccccattcc taaacaaaca gaataagcaa aaaaagcatt gatcccaaga
    38641 gcaggtgccg ttccaattgg ataacgggct aataccccca ttaaaacaca gccgatcgca
    38701 ctggctaatg cagtcgcagt aaacactgct ccttcatcca ttccagaagc acctaataca
    38761 gttggattta caaataagat ataagccatt gagataaatg ttgtaaatcc tgccagtacc
    38821 tcacgtttta ccgtggtgtt caatttttct atttcgaagt aggaaagtat tttttccttc
    38881 atcttccatc cccctaaatc cgaacattat aaataaagtc cgttaaaatt tcgatttatt
    38941 aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa aaaattcgga
    39001 ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa gttcatgaac
    39061 attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc attctagtct
    39121 caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt tgatgaattc
    39181 ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga ccatttttcc
    39241 ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct tctcactatg
    39301 ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca ctgtttttcc
    39361 tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg ccttcagggt
    39421 cagaaaaatt gaaagtcaag atgccattgt tatctgtgat ttctcctggt tcttccatgt
    39481 tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt ggtcctaata
    39541 cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct aattcttttg
    39601 agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca gaaatttctg
    39661 caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca taaagcatca
    39721 cacgtgtttt ttgtatcatc atttgaccct tctttctatt tgattttctt tcctgtctat
    39781 aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact attcaagaaa
    39841 aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca aaaaaaaaac
    39901 ggcaatgaat gccgctcttc ttatttcgat tcttttataa ttctgcttct gattcatctt
    39961 tggttagtgt gtgtctaaca ctagttacgc caaatgtttg atcgattttt tccttcaacg
    40021 cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt gatggattca atgaccagca
    40081 ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag ttgagacagc
    40141 gacagatcgc tccttgttca tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg
    40201 aatctgcacc taaaggcagg aatgattcgc caccttgggc ttttcctaaa tgcagatttc
    40261 ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc aaaagacaag gaaatactat
    40321 tatagatatc tacaagcgga ttgattggga aaaattcccg ttcttgattt gcacgtttca
    40381 gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt gaatgtggta aatgcctgac
    40441 gccactgagc aatgacttcg ttttggctga atgtttcttc tgtaaggtaa tttcttgctt
    40501 cttttgctgc ctcattcaat aattgatgat acgtttcttc actttctttt gttacgtggt
    40561 tatcgatacc atttacggat aagatttcga tggttgcttc aggaaatact tcccagaaat
    40621 tggtatccac tattactttt ttcatctgtt cacacctctt gattagaata attgcttgat
    40681 gaatagatag tctgtttggg gatcatctcc catataaaaa acatgttcgc tgtatttgat
    40741 aaatcctttt ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa caccgagcca
    40801 aatggctgtt ttttgctttt cttttgcttt ttcaatagct gtatctagta aaaatgtacc
    40861 gataccttga tgtttaaatt tttttcgtac atagattcgc tcgatttcta aaaaatgatc
    40921 agccatttcc tcagattgac tcgttcctgt atttagtttc aggtaacccg caggctcttg
    40981 atcgatactc actaggaaaa aatcggtttc tttatctttt agctcttctg taagtttttc
    41041 tagtgcataa gcttgtgtca agtaatcagc taaatgctcc ggtgaattat cttttccaaa
    41101 tgtatctgta aatgtttcga tactaatttg ttgtagtaag tctacatctg aaggcgtcat
    41161 ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa tagatccttt tattgccttt
    41221 tttgacaaaa ttccaatctt cgctaacatt cttgcatact ttatttaaaa gatattccaa
    41281 ttgttttgct tcttcgtcag aaagaccatt taatgcgact gcattggagt agttgttttc
    41341 tcgaatgatg aaaggataga tttctgctcc ttttttagtc ggatagagtt tcttgatttt
    41401 tttattttct ttatcttcca aacgttcgat catcccattg gattctaatt tcttgattgc
    41461 tcgggcagtc gttgtacgat ctactttgat catttctgct agtttttctt ggatgattcc
    41521 aggattttcg caaatacgta ctaaataaag atactgtcct cttgttaaat cgacttcttt
    41581 gaattcgata ttgctaatag aatctagcgc tcgggcaatc attccgatgt ctcgaaggat
    41641 ttcagccatt tcatcccacc tttattttgt ttgtaagact agttcacctc tttttgttgc
    41701 aaatgcaact attttcaata aaaaaatcaa gttctttttt aataactagt aaagtatatt
    41761 cttttcttct tttacgaaaa agctcaatca caatagatct ggtttttgtt ttctaaaata
    41821 agtaactgtt tcttcgttgt tcgcttttct gatagctttg gcaactcttc tatactaaaa
    41881 aatcccatgt tggaagtttc attattctca ataaacttgg cctcttcacc gtggatcgca
    41941 caagcaaaaa caagtttata atactgaaaa agctgtggaa tatctttttg tttattcgta
    42001 tcaaaaactg ctcgcaactg agtagtttct actgttaatc ctgtttcttc atagacttct
    42061 ttacgcacat tttcttcagg agacaatcct atttcagcaa atccaccagg aagtgaccat
    42121 tctttcgtcc gtttatcttc gattagtagg accttgccat cttttttgat ataggcacga
    42181 acatctactt ttggtgttgg atgtccttct ccaatcgttg tgagcgcttt tatttcttcg
    42241 aaagtttctt ttcctatttc actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa
    42301 cgctctttat caaatacatc ttttccataa tatagaccag cttgagaaag tgcgagtagt
    42361 cttttgtatt gagccaactt cttgttcatt ctcttccctt cttttttagc aaatgtatga
    42421 ctatgtaatc aagtatagaa gactggttta attacggcaa ataaatataa tgaaatctgc
    42481 aataaataaa agaatgttaa aaaactcgag aacaaaagac attcgacttc tctggggctg
    42541 tgaataaatg gcgttcaaaa gcttgcatct gcggtaataa gccccaccaa acgaaaaata
    42601 tgacgagcta ttcttgtctg gctgttctta ttaattgagg caggacagct ttttcacaac
    42661 ctccccacta aacggtgttc tcctaccaac tcctcggtca caagtcacaa tattcatcaa
    42721 acatgagaag ctgttttttt gaaattgctc cttgtgactc ggagctgaac gacaggctca
    42781 cgacctctcg gtaaacagcg ttcaaaagcc aatttttcga tattaagcca tttctttgtt
    42841 acttattgtc attgatttct ttttctaatt tatccaatag tggagtgtaa tccacttgag
    42901 tatagtctgt tacatcaaat tcatatagtg ttcctaactg gaatttgtca tattttcggt
    42961 cggcgatgat ctttttgaat gcttctttcg taatatgatt ggtccctttc gttcgatctt
    43021 ccaatacgtc accatagtga taatgatcca tgatatagct caaatgacga tctgattcac
    43081 gatcacgttt gtaccgacgt tgccataaca cttcaaagtc tgctgttagg cgaatcgtga
    43141 tcacctgata tccgtatcta tctgctaatt cttggagtcg tgctttttgc ttatcactaa
    43201 aaggatactc ggacacaatc actttctttc ctgcttccat atacaaatca agtgctttgt
    43261 aatagaaagg ataaactttc gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc
    43321 cgatggattc tgcatagtat tccttgattt catcgagcgt gatggcatac atatctggaa
    43381 atcgttcccg tagtttattc ataagatatg tttttcctgt accaggacta ccagccagaa
    43441 gaatgaaata ctttttcatc tcatttattt cctaagttaa ttggactttc caagttcata
    43501 aacagtccaa tcttatgctt gacaatttct tttactgctt catttgctgg aatcacatta
    43561 tggcgaagag acttgttgat ttctgtttca ctaaaggttt ctttagctac tttcgtccag
    43621 ttgactaaaa gctccgttcc aacattgaat ttgcggatcc cgttattgat caattcagga
    43681 taatcttctt ctttcacacc tgttcctcca tggataacta gggggacatc aactactgca
    43741 tcgatttctt tcagcaaggg aatgtttact tctgtttttg atttgaattg tccatgattc
    43801 gtaccgatag caatagctaa tgcatccacg cctgtagctt ctacaaattt cactgcgtct
    43861 tccggtcttg tatatacctt gtcattttca gcgacatgga ttccttcttc tgtaccgccg
    43921 attgttccaa gttctccttc tacggaaaca ccgtgcgcat gggcatattc tactactgct
    43981 tttgtcttca ggatattttc tttgaaaggc agatgggacc catcatacat gacagaagta
    44041 tatccgctgt cgattgcttc tttgatatca tcaaagtctc gggcgtggtc taagtgcaag
    44101 actgcatcaa ccatttcttc ttctgcaata gctgtcatta cattgactaa cacatgcatg
    44161 ccgatatatt tagcggtatc gacacttgtt tgaataatga tcggcgaacc catttctttt
    44221 gctgcgccta gcatagcagg aagcatttct aggttatgcg tattaaaagc tccaactgta
    44281 aaattcaact cttctgctgt ttttgttact tctttcaatg ttgtatacat cttttattgc
    44341 caccttatct ttttatttgt ttcctttgat cgttacttgt tttgcaattt gtcgcatctc
    44401 atccattttg cccatgtagt aatcgatccc ttgatcatca ccagcacgag cagcatctgc
    44461 acgcttctgg ttatagaggc tcatcagttt tttataccca tctccgatcg tctgtttatt
    44521 ttccaaactc ttttctaaat attggatggc ttgatcgttc acatacaatt cagaaaattt
    44581 aagtccaact tgttcataaa gtttcgctaa tttatgctta tcttgggtag tggaaatctc
    44641 ttgtttcaat tgtgtaatct ctgattgaat cttttcggtc gcttctggcg atagggcttt
    44701 ttcttctttt gccagaactt ttttcggttc tttttctttt ttcttaaaaa atccaaacat
    44761 cattttccct actttcaatt aaaatgcttt caaaactggg cttaagatcc aggcatataa
    44821 gagtgctgcg gctactgtat ctacatcggt tgctgtagca ttgttgaatc ccatcgaatt
    44881 aaagatcgtt actagcaaag cgggcaataa tgtaataaag aaaccatgag caataccgcc
    44941 aatgatcgct cctcggcgtc cgccaactgc gttgccaaat atccctgctg tccctccggc
    45001 aaagaaattg gttagcatac caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa
    45061 catggcaata atcgtaccaa tcgttgtcgt aatgaaacct aaaatgacag catttgggct
    45121 ataaggaaag aaaactggac aatctaaggc agggatcgca tcaggaacta atttcatagc
    45181 tatccctcga aatgccggca cgatttcacc taacagtaga cgtacaccag ctagcaatac
    45241 atacacaccc actacgaatt ggatggcttg taaaaaggca aacatgatat agttttgatc
    45301 tcctgataat tcagaagcga atggttcgcc tgcaaataaa acagtgatga tatatagcgg
    45361 gaccatcacg accatgacag acaaataagt atcttgcaga aattcgaaat gtgtcggcaa
    45421 cttgatatct tcgatcgact ttttattttc gcctttctca ccaaaaagct tagcaacacc
    45481 ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt
    45541 gattttacga atgatcggtt gcgctactgc cggcatagca atggcgaaac aagctccgat
    45601 aaaaccaccg accaagatca atacgatccc tcgcagaccg gcaaaatagc caaagactgt
    45661 cgtcatcgtt gccatccata aaattgcttg accagttaaa aaaatgtact tccattttgt
    45721 gaaacgagcc aaaataatat tgaatacaaa aatggctaag aacgtcagtg caatatctct
    45781 acctaaaccc aaatcattca ttgcttgacc attgatagat tcaatagaag ggatgatccc
    45841 ttgcatatga aatccttcag taaagatttt cccgaaataa gttaaacttc caacgatgat
    45901 gctcgaaccg gcactcaaca cttgaaatcc taggagtgtc tttaacgtgc cagagatgac
    45961 ttgtcctgct gatttcttct gcagtagtag acccaacata gcaatcaatg caatggtgat
    46021 cgatgcctga gtcaaaatat tttcgatgat aaaattgata acgcccatg
//
LOCUS       DASNNU010000010        45477 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.010, whole genome shotgun sequence.
ACCESSION   DASNNU010000010 DASNNU010000000
VERSION     DASNNU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.010"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(234..719)
                     /locus_tag="U8116_000545"
     CDS             complement(234..719)
                     /locus_tag="U8116_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEN8433561.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    809..1154
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1199..1840
                     /locus_tag="U8116_000546"
     CDS             1199..1840
                     /locus_tag="U8116_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN8433562.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1859..2494
                     /locus_tag="U8116_000547"
     CDS             1859..2494
                     /locus_tag="U8116_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN8433563.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2512..3327
                     /locus_tag="U8116_000548"
     CDS             2512..3327
                     /locus_tag="U8116_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN8433564.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3646..5082
                     /locus_tag="U8116_000549"
     CDS             3646..5082
                     /locus_tag="U8116_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN8433565.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5271..7718
                     /locus_tag="U8116_000550"
     CDS             5271..7718
                     /locus_tag="U8116_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN8433566.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7948..9015
                     /gene="rlmN"
                     /locus_tag="U8116_000551"
     CDS             7948..9015
                     /gene="rlmN"
                     /locus_tag="U8116_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN8433567.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9208..10311)
                     /locus_tag="U8116_000552"
     CDS             complement(9208..10311)
                     /locus_tag="U8116_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN8433568.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10864..11412
                     /gene="aac(6')"
                     /locus_tag="U8116_000553"
     CDS             10864..11412
                     /gene="aac(6')"
                     /locus_tag="U8116_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN8433569.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            11958..13619
                     /locus_tag="U8116_000554"
     CDS             11958..13619
                     /locus_tag="U8116_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN8433570.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(13689..14036)
                     /locus_tag="U8116_000555"
     CDS             complement(13689..14036)
                     /locus_tag="U8116_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEN8433571.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            14120..15061
                     /locus_tag="U8116_000556"
     CDS             14120..15061
                     /locus_tag="U8116_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433572.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            15062..16105
                     /locus_tag="U8116_000557"
     CDS             15062..16105
                     /locus_tag="U8116_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433573.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            16111..17181
                     /locus_tag="U8116_000558"
     CDS             16111..17181
                     /locus_tag="U8116_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8433574.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            17171..18124
                     /locus_tag="U8116_000559"
     CDS             17171..18124
                     /locus_tag="U8116_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN8433575.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            18136..19146
                     /locus_tag="U8116_000560"
     CDS             18136..19146
                     /locus_tag="U8116_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN8433576.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(19431..20252)
                     /locus_tag="U8116_000561"
     CDS             complement(19431..20252)
                     /locus_tag="U8116_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN8433577.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(20349..20627)
                     /locus_tag="U8116_000562"
     CDS             complement(20349..20627)
                     /locus_tag="U8116_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433578.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            20895..22904
                     /gene="metG"
                     /locus_tag="U8116_000563"
     CDS             20895..22904
                     /gene="metG"
                     /locus_tag="U8116_000563"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEN8433579.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(23383..23970)
                     /locus_tag="U8116_000564"
     CDS             complement(23383..23970)
                     /locus_tag="U8116_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433580.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            24145..25467
                     /locus_tag="U8116_000565"
     CDS             24145..25467
                     /locus_tag="U8116_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN8433581.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            25622..26398
                     /locus_tag="U8116_000566"
     CDS             25622..26398
                     /locus_tag="U8116_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN8433582.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            26391..26960
                     /gene="rnmV"
                     /locus_tag="U8116_000567"
     CDS             26391..26960
                     /gene="rnmV"
                     /locus_tag="U8116_000567"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEN8433583.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            26953..27837
                     /gene="rsmA"
                     /locus_tag="U8116_000568"
     CDS             26953..27837
                     /gene="rsmA"
                     /locus_tag="U8116_000568"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEN8433584.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            28288..28917
                     /locus_tag="U8116_000569"
     CDS             28288..28917
                     /locus_tag="U8116_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433585.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(29041..30150)
                     /gene="ugpC"
                     /locus_tag="U8116_000570"
     CDS             complement(29041..30150)
                     /gene="ugpC"
                     /locus_tag="U8116_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEN8433586.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            30359..30781
                     /gene="mgsA"
                     /locus_tag="U8116_000571"
     CDS             30359..30781
                     /gene="mgsA"
                     /locus_tag="U8116_000571"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEN8433587.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            31104..31640
                     /locus_tag="U8116_000572"
     CDS             31104..31640
                     /locus_tag="U8116_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN8433588.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(31680..33443)
                     /locus_tag="U8116_000573"
     CDS             complement(31680..33443)
                     /locus_tag="U8116_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8433589.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(33446..35161)
                     /locus_tag="U8116_000574"
     CDS             complement(33446..35161)
                     /locus_tag="U8116_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8433590.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(35940..36164)
                     /locus_tag="U8116_000575"
     CDS             complement(35940..36164)
                     /locus_tag="U8116_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433591.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            36355..39132
                     /locus_tag="U8116_000576"
     CDS             36355..39132
                     /locus_tag="U8116_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN8433592.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            39213..39632
                     /locus_tag="U8116_000577"
     CDS             39213..39632
                     /locus_tag="U8116_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEN8433593.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            39644..40114
                     /locus_tag="U8116_000578"
     CDS             39644..40114
                     /locus_tag="U8116_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN8433594.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            40131..40910
                     /locus_tag="U8116_000579"
     CDS             40131..40910
                     /locus_tag="U8116_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN8433595.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            40897..41718
                     /locus_tag="U8116_000580"
     CDS             40897..41718
                     /locus_tag="U8116_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN8433596.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            41735..42784
                     /locus_tag="U8116_000581"
     CDS             41735..42784
                     /locus_tag="U8116_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN8433597.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            42797..43801
                     /locus_tag="U8116_000582"
     CDS             42797..43801
                     /locus_tag="U8116_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN8433598.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            44434..45453
                     /locus_tag="U8116_000583"
     CDS             44434..45453
                     /locus_tag="U8116_000583"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN8433599.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        14917 a         7955 c         9417 g        13188 t
ORIGIN      
        1 aaaagaataa aattccgatt tataatcgaa aaaagaagca tttttatata tgcttctttt
       61 ttcgtagaat caataaatat ggatttggct aactatggaa gaatactcgt attagttttc
      121 agcgttcata ctagttgtta cattgtttga ttggccaaaa gaatttctga tatattttgt
      181 gctaaataaa gaaaaagacg cttgaattgg gaaataagaa aactactcta tcattacaaa
      241 ttttcataaa ctggcagatg ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc
      301 ataatcaacg tagtggtatt gatatttgga tgaaagccta cggtatcttg acgatcaata
      361 gcagcatcga tcacgacttg gatttttttc tctttgtcgt gcatcaatgc taatggagtc
      421 actgtacctg ctggaacgcc taggaggtca ggaagctgtt cttcagaaac aaaagataag
      481 cgcttttctt ttaattgttc agctagttgt tttaaatcgg cgtgcttttc atcaggcaag
      541 atgacaagat agatttgttt tccttttctt gatttcaata atagattttt tacttgcgga
      601 ccaggtaggg aaaaagggac atttttgact gacgtaatcg caggatgatc gactttttga
      661 aaagagatac ccagtttttt tagtagttca taagcttctt gttcggttgc acgactcatc
      721 gcgcatcacc ctcctttttt tattagtgta tcataaaaaa gagttgacaa atgaaaaata
      781 atcatgtaaa ttacacgtaa agttaaaaca aatgacatcg aaaagaagag tagccgttag
      841 gtgtttttag agagtctctg gttggtggaa agaggcagca gttaaccgtg aaacacatct
      901 tggagtcgat tttctgaagc gtatagtagg aaaatccggg gagcccgtta cagctgcggt
      961 cgttgctaga tgaccagata aggctggctt ttccgtgagg agtcggtaaa aaaaggtgga
     1021 accacgtgat taacgtcctt tgatcttcgg atcaaaggac tttttgtttt catctgaacg
     1081 gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa acagaggtgg aaccacgtat
     1141 aatacgtcct cttggcattt gccaagagga tttttttatt ttaaaggagg aaaagaagat
     1201 ggagtatttg attgaagtta tgccgcaatt attagtagga gcactcacaa cattgaaatt
     1261 atttttattt accttgattg gctcattacc attagggatt gttttagcat taggattgtc
     1321 tttgcatttt aagccgctac gctacttgct tcaaacatat gtatgggtta tgcggggaac
     1381 accgctgttg ttgcaactga tttttatttt ttatgggttg ccgacgattg ggatcgtttt
     1441 tgatcgtttc gaagcagcac tcattgcctt tatcttaaac tatgcagctt attttgcaga
     1501 gatctttcgt ggagggatac aatcgattcc tcaaggtcaa tatgaagcag cgcaagtttt
     1561 gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc ccacaagtga caaagattgt
     1621 tttaccttct gtagggaatg aagttatcaa cttggtcaaa gattcttcct tgatctatat
     1681 attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg atggaacgcg acgcgacttt
     1741 actgcctttg ctaggcgtgg caatcatcta tcttacattg actggtattc taacaatcat
     1801 aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa ctgataggag gaagaaatat
     1861 gattgaattg aagaaagtga acaaagcatt tggtgaaaaa aaaataatca aagatttgaa
     1921 tctaatcatt ccagatggga aaacattagc tatcgtaggt ccttccggtg gcggtaaaac
     1981 cacgctttta cgtatcttgg ctggcttaga aagttctgac agcggagaat tttatttaga
     2041 cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa caagttgtag gagtcgtatt
     2101 ccaagacttc cagctatttc cacatctgaa tatttttgaa aacattacct tagcaccaaa
     2161 aatggtattg aaacagacaa aagaagaata caccaaaaaa gcagaagaat tagcggcttc
     2221 attagggatt agtgatttat tgaatatcta tccttatcag ctttctggcg gacaaaaaca
     2281 gcgggtagct attgccagag cattggctat gaatccacgg gtattggctt atgatgaacc
     2341 aacaagtgct ttagatcctg aattacgcca gcaagtcgag cgtttgatcc tatcattgaa
     2401 agaagatggg gtaacccaaa ttattgtcac ccatgattta gtatttgcag aaaatgtcgg
     2461 tgatcaatta ctaaaagttt ccccaattca ttaagaaagg agaagataga gatgaaaaaa
     2521 accagttttc tattcgcttt gatcgcaggt cttcttcttt ttgcaggctg ttcgaacaaa
     2581 aaaacttcag cagataattg ggcgactatc aacgaagaga aaaaaatcgt tataggttta
     2641 gacgatacat ttgttcctat gggatttaga gataaagacg ggaagctcac aggatttgat
     2701 attgatttag ccaaagcagt ctttgaacaa tatgggatta cggtagactt tcagcccatt
     2761 gactggtcaa tgaaggaatt tgaactgaac aatggaacga tcgatttgat atggaacggc
     2821 tattccaaaa cgccggcaag agaaaagaaa gtgcaattta caaaacctta tatggaaaac
     2881 gatcaagtgc tgatcacacc taaagaatca ggaatcacaa actttggaca aatgaataat
     2941 aagagattag gtgcgcaaaa tgggtcatct ggttatgatg tatttacgaa acaaccagaa
     3001 gtattaaaag agatagtcaa aaaccaagat gctgttttgt atgatagttt taatgaagca
     3061 ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga tggataaagt atacgcagac
     3121 tattatttga aacaaagaaa cgaactagaa gacttcaaca taacaaaagg ttcttatgag
     3181 agtgaggatt ttgcagttgg tgcacgcaag tcagatagta cattagtgaa aaaaatcaat
     3241 gaagccctag atcaactaga gaaaacaggt gaattccaaa aaatatccga taaatggttt
     3301 ggcgaagatg ttacgccgag aaattaacaa ataggggact gtgacaaaag acgtgtcaca
     3361 gtcccttttt ccgaataaac agtgttctcc tgccaactcc ttgtgactcg gagctgaatg
     3421 gcaggctcac aacctcttgg taaacggtgt tcaaaagcca gtttttcggt attaagccaa
     3481 atttatgcaa aaatgtagaa agccattttc acaaaatttt tcttactact cgaaactaag
     3541 cgactttttc acaacctcct ttacataatt ttattttgtt gtggaagggg tttcgtgtta
     3601 ttctaaaaga ggaacgattg aaggttatag gaaaggaaga gatgaatgga acacaaagaa
     3661 cttacctcat ttccaaaaga ttttctgtgg ggctctgctt ctgcagcgta tcaagtagaa
     3721 ggagcttggc aagaagatgg gaaaggagaa tcagtctggg atcgatttgt acggatacct
     3781 ggaaagacat ttaaaggaac gaacggtgat ttggcagtag accactatca tcgttataaa
     3841 gaagatattg ctttaatgaa agaacaaggt ctgaaagctt accgcttctc tgtagcttgg
     3901 acacggattt ttcctaatgg acgaggagaa gtcaatcaag ctgggctcgc tttttatgag
     3961 agattgatcg acgaattgat tgaaaacgat attgaaccta tgttgacttt atatcattgg
     4021 gatcttccac aagcattgca agatgaatat aatggttggg aatcacgtca gatcattgaa
     4081 gatttcactc attatgcaga aacattattt gaggcattcc gcgggaaagt ccattactgg
     4141 attagtttga atgaacaaaa tatcttcact tcattaggtt atttgttagc agcacatccg
     4201 ccaggtgtga cagacccgaa acgaatgtat gaagttaacc atattgctaa tttagcaaat
     4261 gcatctgtca tcaataaatt tcacgagtta gaaatgcctg gaaaaatcgg accaagcttt
     4321 gcgtatacgc caaattatcc aattgacagt aatcctgaaa atgttctggc tgctgaaaat
     4381 gcagaagacc tgatggccaa ttattggatg gatgtctata tgtggggaaa atatccgatt
     4441 gctgccatga ggttcttaga agaaaaagga tgggcaccaa cgattgaagc tggcgatgca
     4501 gaattgctag aatctgctaa accagatttt cttgggatca attactatca aacggcgacg
     4561 aatgcattca atccattaaa cggagttggt gctggaaaaa tgaatacgac aggaaaaaaa
     4621 ggttcatcag aagaaactgg agttcctgga atgtataaaa aagttgagaa tccatttgtc
     4681 gaacgaacaa attgggactg ggagattgac ccagaaggat taaggatcgg actgcgtcgt
     4741 atcacaagcc gttaccgcat gccggtactg attacggaaa atggcttagg agagtatgac
     4801 aagttgacag aagataaaca aatccatgac gactatcgaa tcaattactt acaaagccac
     4861 gtcaaagcca tcaaagaagc aatttcggat ggagcagaag tcctaggcta ttgcacctgg
     4921 tcttatacag atttgcttag ctggctgaac ggttatcaaa agcgatatgg atttgtatat
     4981 gttgatcaag acgaaacaca agaaggatct ttggaacgat ataagaaaga cagttattac
     5041 tggtaccaaa aactgattga agaaaatgga gagaaccttt agttcacata cttaaaacaa
     5101 gaaggtacgg ctggatatgg ccgtgccttt tcttgataaa atcatttaga aattaagaaa
     5161 aactgtaaaa taagctgaaa aatgaaagaa agcgtcagtt ctttcaaaca aaactttagt
     5221 tcccttactt ttttttgtat aatgaaactt gatgaaatga gggataataa atggcacaaa
     5281 agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc
     5341 aggaacaaat gctgacgatt ggtgttgggt ttgccttgat tttatttgct gtcttcggct
     5401 ttttaaaatt aggtttttta ggaatattga ttgcaaacgg ctttcggatc attgcaggaa
     5461 atacttacca aatcctctgt cttttattag ccgtattagg attttggatt gtcattaaga
     5521 atacagagtt ttcaattggt aaaaaccgac gctggtttgg cggcatactc ttttatttcg
     5581 gtatcttatt attgttacat gcccatttat ttggcaaact ccatactggg gaaccaaata
     5641 tcatggggac gacatgggat ttgttagcca gtgatataaa acaaagtcaa gtagacaata
     5701 acgttggtgg tgggatgatt ggagcaatcc tttatcattt tacttacttc ttgattgcac
     5761 agcctggaag ttatttagca gcagtgctat tactggcagg aggagcattc ctgttcagta
     5821 atctggaagg ctatcagctg ttaaatggta tccaatcaat tggagaacga gtacaggaat
     5881 tattggaagg tgatcctgcc aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga
     5941 aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc aaaagaagct gcagaaaaag
     6001 aagctgcaga atatgagaag aaaaaagcgg tgagccagca aagggaaaat actgctgatg
     6061 aatggcagga accagaaaat acagagccgg agcagctaag ttttgttcca atcgacagtt
     6121 ttcaagaaaa tatccatcct gcgaatctag agaaaccagt accagataca ccaaaacaga
     6181 ccaatacagc agaaggtttt tcagatgagt tgccagagga tgacggtacc tcattagaat
     6241 ttgagattga agcagaacag gaaaatcaag actatgaatt accgacagtt gatttgcttg
     6301 attctatacc aactgtggat caaagtgatg agtataaaaa aattgaaaaa aatatcggcg
     6361 tattagaaca aactttccaa agtttcggtg tcgatgccaa ggtcgtcaaa gccagcttag
     6421 gaccatccgt cacaaaattc gaagtccagc cggctgtagg ggtaaaagta agtaaaattg
     6481 tgaacttaac agatgatatc gcgcttgctt tagcggcgaa agatgtccgg atggaagcac
     6541 cgattccagg taaatcactg attggtattg aagtaccaaa tggaaagatc agtatggttt
     6601 cctttagaga aatcatcgaa gcacaaccaa accacccaga taaattacta gaagtacctt
     6661 taggacgtga tgtttctgga cgggtacaaa cggctgatct ttcgaagatg cctcacttac
     6721 tcgtggctgg ttcgactggt agtgggaagt cagtagcaat taatgggatt atcacaagta
     6781 tattaatgcg tgctaagccc catgaagtaa aactgatgat gatcgatccg aaaatggttg
     6841 aattgaatat gtacaacggc atacctcatc tgttgacgcc ggtcgtgacg aaccctagaa
     6901 aagcagcgca agccttacaa aaagtagtac aagaaatgga agaacgatat gaaaaattcg
     6961 cggcaaccgg agtaagaaat atttctggtt acaatgaatt tgttcaacag aaaaacttgg
     7021 aaaacggaac gaaacatcca accttaccat tcatcgtagt gatcgtagat gaattagctg
     7081 atttgatgat ggtagccagc aatgaagttg aagatgcgat tattcgttta gcgcaaatgg
     7141 ctcgggcagc ggggattcat atgatcctag ctactcaacg tccaagtgtg gatgttatca
     7201 caggaatcat caaagcaaat gtaccttcca gaatggcatt tgcggtatca agcggtacag
     7261 attctcggac gatcattgat tctaatgggg cagaaaagct cttagggcga ggagatatgc
     7321 tattcttgcc aatgggtgag aataaaccga tccgtgtgca aggagcattc atttctgacc
     7381 acgaagtaga acgggtcgtt caatttgtta cagatcagca agaagctcat tatgaagaga
     7441 aaatgatgcc gacagatgaa gttgaaacga caggtgctcc agaacagcca caagatgagc
     7501 tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac ggcaagtatt tcccttctcc
     7561 aaagaagatt tcggatcggt tacaaccgag cagcacgttt agtagatgaa ctggaagctc
     7621 atggagttgt tggcccgtca gaaggaagca agcctcggaa agtattcatt gagcaggaag
     7681 aaacatcaga aacgttgccg gacgataatg acatgtaaaa cttgtgtgat gaagggttga
     7741 aaaacgaata accaataaaa agacaaggca tgatgatacc aaaagaaaaa atggtttttt
     7801 tccatctttt ctaatacggt atgatgtctt gtctttttag tttttgtgag attaaagttc
     7861 tattcgattt atttgtgaag tattgcatta aggaaatgaa tcatgtatag tatgtagcga
     7921 aactttgaga aaaagaggga aaatactttg gaaaaacagt ccatttatgg cttgacgaat
     7981 gaagaattaa tcaattggtt tatagaaaac ggcgagaaaa aattccgagc tgcccaagtt
     8041 tgggaatggt tgtatcaaaa acgtgtaagt aatttcacag aaatgacgaa tctatcgaaa
     8101 caattgatcg aaaaattatc ggagcatttt atcatcaatc ctctaaaaca gatggtcgtc
     8161 caagaagctt cggatggtac agtaaaatat ttgtttgaac tccctgataa aaacatgatt
     8221 gagactgtac tgatgagaca agaatatggc ttgtcagttt gtgtgacgac acaagtagga
     8281 tgtaatatcg gctgtacgtt ttgtgcaagc gggctgctca aaaagaaccg tgatctgact
     8341 gctggagaaa ttgttgcaca gatcatgatg gtccaacatt attttgatga gcgcaatctt
     8401 ggcgaacgcg tttcacatgt cgttgtaatg gggatcggtg agccatttga taattacgac
     8461 aatgtaatgg actttttgca tatcatcaat gatgccaaag gattggcaat tggtgctcgg
     8521 cacattacag tttccactag tggattagca cacaaaatca aggaattcgc taataacggc
     8581 ttgcaagtca atctggctat ttccttgcac gcgccgaata atgaggtgcg aacatcgatc
     8641 atgcggatca accgaagttt cccaattgag aaattgatgg aagctgtgga tgagtattta
     8701 gagaaaacaa accgtcgaat cacgtttgaa tatatcatgt tgaatcaagt aaatgataga
     8761 ccagagcatg cgcaacaatt agcagatcta ttaaaagata agaaaaaatt agcatatgtc
     8821 aacttgattc catataatcc agtcagtgaa catgatcaat attctcgcag tccaaaagct
     8881 gatgtattga gattttatga tgtattgaag aaaaacggcg taaactgtgt gatacgtaaa
     8941 gaacatggta cagatattga tgcagcttgc ggacaattgc gaagcaagca aatgaaaaaa
     9001 acagttgcca aataatcgta tcctactcgt tttttcaatt accataaaag gagcagtttt
     9061 cttaattaag gaactgttca acttaaataa gagaaaagat tctatagcga aagctataaa
     9121 gctttttttg acaagaaaaa ccctcatcaa gcaaagaaat gtcctcaatt tgatgacagc
     9181 ctgcttgatg ggggttttag caattattta actgattttc ttatatcctt gctggctttc
     9241 ttgtgtagca tgataagttt ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat
     9301 ttcataaggc acatgtttaa tatcatttgt tgccatggct ttatctaaga ttgcttctaa
     9361 atcggtcgtg acattgagat tcatgtcggt taacgatact ggaagttggt tctttttcat
     9421 aaacacatag attttatcaa acaaatcgaa ctgctgatcc atcatcaata aggtcagtac
     9481 accataactg acagatgctc cgtgaaggta tccctccatt ttttctagaa cagtcgtacc
     9541 gtaatagaat gagtgtgcca tagcagaatt atagttattg ttaactaaaa cagaaaccaa
     9601 tccggtagta acaatgatat ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc
     9661 ggcactatct ttcaatgctt gcgtaccata agtcaagaga cgatcaatgc acatttttcc
     9721 gatttggatt cctaatctat ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga
     9781 ttcgacttct ttggataaag catcgccaat acctgcccat aagtatttat caggtgcttc
     9841 ggcaataatt gctgtatcaa tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc
     9901 gtccattacg ccttcttcgt tatagatgac acataccgca gtgacgggcg aacagttcga
     9961 accaatagtg ggaaaggtga agactggttt agctagtttt tcaccaatga cttttacagt
    10021 atcgcaagca cggccaccac cgactgcaaa caggacgtct gctttttgga cactttcatg
    10081 ctggatcaac tgttcaatat ttccataagt tgaattaccc ccgtaccaga gactggcgac
    10141 aacctctatt tcctgtgtca aagctacttt tattttgttt tctgcctttg aaagtgcagt
    10201 cttccctcca atcagcacta cttttgtccc atattctttg acgattccat ttgctttttc
    10261 atacgcatct gttccaatgg tataatttgg taagaaaaca ctttgactca tattcatttc
    10321 cttctttctt cgttgaaacg ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt
    10381 agatggagta gaggtgaacc taagacagga taagtgatga ataaggtggc atgaacacct
    10441 tgaaatcatc aataagtatc ctaaaaacga acaagtacaa aaacattgaa aacgacaata
    10501 atgaattttt gttcctctta gtatttctac cgattccctt tgcttttttg taattatcta
    10561 aaaattctaa aaattaagat tattatatga aaacaggttt tatttgtcaa ttcataattg
    10621 gtttttttta aagcaaaaga taatctatat catgatgaac aaacagagtt ttttcctcca
    10681 attgatggct ttcctgatct ttcctatttt gaataaagtg tttctgtata atttttttat
    10741 tttataagta agataaataa tgaaagaata gagatactga ttggtagcta aagatttttg
    10801 agcgagaaca tttcgttttt tcaaaaggac tgttagaata agcaagaaca agggggaaga
    10861 cgtatgataa tcagtgaatt tgaccgtaat aatccagtat tgaaagatca gctttctgat
    10921 ttactgagac tgacttggcc ggaagaatat ggagacagct cggcagaaga agtagaagaa
    10981 atgatgaatc cagaacgaat cgcggtagca gcggtagacc aagatgagtt agtaggattt
    11041 attggtgcaa tccctcaata cggtatcaca ggttgggaat tgcatccatt agttgtagaa
    11101 agctcccgac gaaagaacca aataggtact cgattagtca attacttaga aaaagaagta
    11161 gcttccagag gaggaatcac gatttattta ggtacggatg atttagacca tggaacaacg
    11221 ttaagtcaaa cggacctgta tgagcataca tttgataaag tggcttccat ccagaacctt
    11281 cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca
    11341 aatgcaaatg gctgggacaa accggatatt tggatggcaa aaacgattat tcctcgacca
    11401 aattctcaat aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa ataagaagga
    11461 atttacgaaa attacttctt gaatttttgt gaaattccaa cttatttccg aagaagctac
    11521 ttttggaaca ccgtgtacca gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa
    11581 aattttgtga aaatggcttt ctacattttt gcataaattt ggcttaatac cgagaaactg
    11641 gcttttgaac accgtttatt cagataaggg actgtgacaa gacttttgtc acagtttctt
    11701 tttgtttatt cttttttatc tttgtatggc aataatagac aatataacga aaattattta
    11761 atggcttttg gaagaccatg gaaaaccttg tacaaagcta ttttttctga aaaattcctt
    11821 gaaacctcac gaaaaaatca ttattttatg aaaaagacaa aaaaaagtgt tgatttttta
    11881 ataattattc attaaactct taaacaactt cagaaaatat taattattct gaaaattcca
    11941 ataaaggggt acgtaaaatg aaaaagaaat ttacatttgg tttggttgcg gtatgcggcc
    12001 ttgtacttgc tggatgttat ggcggcagtt cagccacaag cagtagttca gcaaaaagcg
    12061 gttcagcaga tggaggaggc gtatttaact tagtcgtgcc tcaagaaatg cctacagctg
    12121 acttatcagt tgcaacagat acaatcagtt ttacagcatt gaataacgtt tatgaaggaa
    12181 tttaccgtct tgatgaagac agcaaaccac agccggcagg agctagcgag ttggcagaag
    12241 tcagtgaaga cggattaact tacaaaatca aattacgaga agacgcaaaa tggtcaaatg
    12301 gtgatcctgt tacagcagcg gattacgttt atggctggca acgaacagta gacccagcta
    12361 cagcttctga atatgcttac ctgtatgcgc cagttgaaaa tgcagaagac attacagctg
    12421 gaaagaaaga taaatcagaa ctagggatca aagcagttgg tgactatgaa ttagaaatca
    12481 aattgacgaa acaaacacca tatttccaat atctgcttgc tttcccatca tttttcccac
    12541 aaaaacaatc cgtagtggaa gaaaatgggg atgcctatgc atctgcaagt gataaagctg
    12601 tttataatgg accattcaca ttagaagatt ttgatggacc aggaacagat acagaatgga
    12661 cttataagaa aaatgatcat tactgggaca aagatacagt aaaactttct gaaatcaaag
    12721 tcagtgtagt aaaagaatct tctactgcat tgaacttatt caaagatggc caagcagatg
    12781 acgtgatctt gtctggtgag ttagcacagc aaaacgcaaa cgatccagct tatacttctg
    12841 ttaaagaagc gcggacaagc tatatcgaat tcaatcaacg tgaaaagaat tcaccattca
    12901 acaatgtgaa cctaagaaaa gctatttctt attctatcga tcgtgaagct ttagtaaaac
    12961 aagtactagg tgacggatca gttgtctcta caggattgat cccatcagat atggtaaaaa
    13021 gtccaacaac gaacgaagac tttgcaaaag aagctggaaa attagtttct tatgacaaag
    13081 ataaagcaaa agaatattgg gaaaaagcga aaaaagaatt gggcgttgat tctttagaat
    13141 ttgatttgat ggcttctgat gatgattcat cgaaaaaagt aatcgaatat gttcaaaact
    13201 caatccaaga aaacttggat ggcgtaacag tcaaaccaac acctgttcca ttctctgtac
    13261 gtcttgaccg ctcaacttcg ggtgattttg acgcagtact aggcggatgg gctgctgact
    13321 atgcagaccc aagcagcttt actgatttgt ttgtcacagg taattcatac aaccgtggtc
    13381 gctggagcaa tgcagaatat gacgaagcag ttaaagcagc ttcatcaaca gacgctggcg
    13441 atgaacaagc ccgttgggat gatttccaaa aagcaatcaa gatcatctct gatgatatgg
    13501 gtgtcgttcc ggtgtaccaa aaagcagaag gtcacctagt gaatgaaaaa atcaaaggta
    13561 tcgttcacca tgcggcaggc gcttcatggg attacaaatg gacatacgtt gaagaataat
    13621 cgttaatacc gtgagcatga gaaaaaacga ggctgcggca aatgtcgcaa cctcgttctc
    13681 atattcactt atcctaacaa agaaaaaagc atgaaaatag cagaaagagc aattagtatt
    13741 ccggcaatga tcagccaaaa gctgtacata ccgtagctgg cagacgagag cgaggaaaat
    13801 tctggttttt tctttcggtt ccttgttcgt gcaagatgga ccgatcgctg aaaatgatcg
    13861 aagaaacccg aagaaaagac ccataaaaaa ccgccgataa tcagaaacgg caatgaccat
    13921 aagaatagat cattagataa ttgaatgagt gtaaaggaat ggttgatgag attttttatt
    13981 aaaataacca gaatcagtag aaaagctact gcgtagggga tagatttttt tttcatttga
    14041 ttgctccttt atttttggtt cattatctaa taatgtagcg aaacatctgt cagaagtcaa
    14101 aagctagggg gaaacattag tgaatagtta tatcaaatat gtattaaaac gtgtcttttt
    14161 catgatcatc acgctttggt taattgcaac aatcaccttt ttcttgatgc aattgctgcc
    14221 gggcacgcca tatacgaatc aggaacgttt gagtcctgaa acaatcgaga tgctaaataa
    14281 acaagtcggt ttggataaac cagttatcgt acaatatggg atttatcttt ctaatttgct
    14341 tcaaggagat tttggaattt ccttccaatt taaaaaccag ccagtcgcac atttgttagc
    14401 tggaagaatc ggaccatcct tgcagttagg gttacaggca attatctttg gaacagtcct
    14461 agggacgatt ttaggaacga tctctgccat gaagcaaaat acatgggcag atacatcttc
    14521 tacactcgta gcgatattag gtcgttccat tccaaacttt gtatttgctg ttttattaca
    14581 atatatcttt gcaatcaaat tacaagtttt gccaatcgca aaatgggatg gctttgtgta
    14641 cacaatctta ccaacaattg cgttggccat gtcgccgctt gctgactctg ctcggtttat
    14701 ccgtacggaa atggtggaag tactgcatag tgattatgtg gaattagcaa aagcaaaagg
    14761 attgagccgt tgggaaatcg cctttaaaca tggtcttcga aatagtttga ttccattgat
    14821 gactttatta ggaccgttag ctgtagcatt gatgaccgga tcactggtgg tagaaaatat
    14881 ctttgctatt cctggtatcg gggaacaatt cgttaagtca atcacaacaa atgattatcc
    14941 tacgatcatg gctgtaacga tcctttactc atttatgctg atttttgtta ttttgatcgt
    15001 ggatctattg tacggtctag ttgatccaag aattcgtgtt tcagaaggga gccgaggata
    15061 aatggaaatg ataccaaagc gttatgaaac agttgcagat atccctgcgg atgaatttct
    15121 tccattacaa aaaaataccg aagaagaacg tgaacagatt actgcacctt ctttgaattt
    15181 tatccaagat tcatggcgcc gtttaaagaa aaacaaagct gccgttattt caatggtttt
    15241 actattggtc atcattttga tctctgtgat cacaatcttt gtttctcctc atgatccaac
    15301 tgcccaaaac gtggcttata tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg
    15361 attgaatgga aaagcaatgg ttggtggtga acttgttgat aaatatgctc aagcaaacgt
    15421 cccttcaaac gtaaatttct ttttaggaac agacggtttg ggacgtgatg tattaagccg
    15481 cttatttatg ggaatgagaa tctcgttgct gatcgcattt attgctgcat tactagatgt
    15541 aacgattggt gtagcttacg ggttggtttc tggtttgtta ggcggacgtg tcgataacgt
    15601 gatgcaacgt tttttggaaa tcttatcagg aattccaaac ctcgttgtca tgattttgat
    15661 gttggtcgtt tttgaaccag gaattttttc aatcgtggct gctatggcca tcacaaactg
    15721 gataccaatg gcacgtatcg ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt
    15781 tttagctgga ttgacattag gtgaatcaag aatgaaaatt gcattcaaac atattttgcc
    15841 gaatatctct agtgtcatta ttattcaaat gatgtttagt attccatctg ctattttctt
    15901 cgaagccttt ttaagcttta tcggtttagg tcttacgcct ccgtcagctt cattaggaac
    15961 gatgttgagc gaagggtaca agacattcct gtatctgcct tatctattat ggattccggc
    16021 agtaacttta tcggttatca tgattggctt taacttactt gctgatggat tgcgtgatgc
    16081 ctttgatcct aaaatgaaag agtgaagacg atgacgaaaa ttttagaagt gaaagactta
    16141 gaaatctctt ttgatacgta tgctggaaag gtacgagcga ttcgcggtgt caattttcat
    16201 ttgaataaag gagaaacatt ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg
    16261 accagaagca tcatgcgctt gctgtcaagc aacgccaata tagatgctgg tcaaatcctc
    16321 tttaaaggta acgatatcgt tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa
    16381 gaaatagcga tgattttcca agatccaatg acctctttag atccgacgat gcccattggg
    16441 aagcaagtcg ctgagtccct tagaaagcat aataaagttt caaaaaaaga gggacaaaaa
    16501 gctgcattag aattgcttaa attagttgga atacccgaag ctgagaaacg tatcaacagt
    16561 tatccgcatc agttctcagg aggtcagcgt caacggatcg tgatcgcgat tgcactggta
    16621 tgttatccgg aaatactgat tgctgatgaa cctacaacag cgcttgatgt gactattcaa
    16681 gcacagattt tggagctgct gaaagaaatt cagacaaaaa tcgatacatc aattatcttt
    16741 atcactcatg accttggtgt agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga
    16801 ggaagaatcg ttgaagttgg aacatcggaa gaaattttct acaacccgca acatccctat
    16861 acttggggac tgcttggttc aatgccgaca ttagatagtg cgaacgatcg attgtatgct
    16921 attcctggtt ctccaccgga tttattgaac ccgccaaaag gagacgcatt ctatccaaga
    16981 aatgaattcg cgatgaaaat cgatgcagaa caagaaccac cattttttga actatcaaaa
    17041 acacacaagg cagcaacttg gctacttgct ccgcaggcac ccaaagtgac accgccagaa
    17101 gaaatccaac gtcgctgggc tatctttaaa gagaaacaaa aacatactta cggtcaagga
    17161 ggaattgctg atgccaaata aactacttga agttaaagga ttgaaacagt atttcaatgt
    17221 aggaaaaaag aatgaagtac atgctgtaaa tgacatcagc tttcacattt atgaaggaga
    17281 aacattcggt ttagttggag aatctggaag cggaaaatcc actactggcc gtacgatcat
    17341 tcgcttgaat gaacctactg atggtgagat attattcgac ggtcaagata taacgaagat
    17401 caaagataaa aaaggtttga cgaaattccg gcatgatgtt cagatgatct ttcaagatcc
    17461 atacgcttcg ttgaatcctc gtatgaaagt cagagatatc attgccgaag gaattgatgt
    17521 taatggacta gctaaatcat ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac
    17581 tgttggattg aatccaagcc acggtactcg ttatcctcat gaattttcag gtgggcaacg
    17641 ccagcgtatt ggtattgcac gagcattggc agtaaaaccg cgttttatca tttgtgatga
    17701 accaatttct gcactagatg tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct
    17761 ccaaaaagaa catcaattga cctatctatt tattgcacat gatttgtcga tggttaaaca
    17821 tattagtgac cgaatcggtg taatgcataa cggcttgtta ttagagatgg gaacaagtga
    17881 tgaaatctat aatcacggtg ttcatcctta tacagaaagt ttgttatctg ctattcctct
    17941 accagatcca gaccatgaga gaaaacgcag aagaatcaaa tatcaacctg aaccagatga
    18001 cggacaagta cgtcaacttc gtgaaatcgc accagaacat tttgtctatg caactgagca
    18061 ggaagtccca tattacgcaa aaaaactaaa acgacaaaag gaatctcttc tggttgctaa
    18121 ctaggaggga catagatgct aaaagctttt aaattccgta tgtatccgac agaagaacaa
    18181 aaacagcaat tgattcgtac ttttggatgt gctcgattta cctataacca tttgctgaaa
    18241 aagcggcaga aaagttggca acaaacagga gtagccaatt tttcgctcac acccgctaca
    18301 ttaaaaaaag aatacccatt cttaaaagaa gtagacagtc ttgctttagc aaatgcacag
    18361 cttaatttgg atcgggcttt tcgcaattat ttcaagggac gtgccagctt cccaaagctt
    18421 aaaacgaaaa aaagtatgtg gcaatcttat acgaccaaca accaaacccg tacgatctat
    18481 ctggaaaatg gctacttaaa gttaccaaaa caaaaagaac taatcaagat aaatcagcat
    18541 cggccagttg aaggatcgat tcgctccgct acgatttctg ctcgatataa tgaagaattt
    18601 tatgtcgcac tactttgtga tgtttcgcca gtaaaaaaag aatctttagc taaatggatc
    18661 gggatcgcct atcatccgaa gacattgatc caaacatctc gacctctaga agtaacttta
    18721 ccaaaatttc accagacaga agaaaagctg caacatgcac agcggaaatt gaatgtgaaa
    18781 gtccgctccg ctcaccatag aaagatcagg ttagatcagg caagcaatta tcagaaacaa
    18841 aagagaaaag taatggatct ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa
    18901 caattatctg ggaaattggt caaacaatat gactacttat ttgtagaatc ttttccaaaa
    18961 gaagaggcac atgctgattt ttctattcac gactggcata aattaataac aaaattgcgt
    19021 tataagtccc aatggtataa taaaaaattt cttttgatca ataccgacgg agcagaagaa
    19081 agcaattccg taagaaagag ccaagtagta gaaaaaatgg gcagacattc ggttatcaag
    19141 ggataaaaaa tgaatgaaac tgataggagc gttagggtta gccttgttaa taaaaagtct
    19201 taatgacttt tgtttctagg aatttttctt ttgaagaaaa gctcactaat tatgacaaac
    19261 caataaaaca atagcagaaa aagttcgtac gagctttttc tgctattgtt aattttttat
    19321 tcagaaagtt tacgaataca tcaaaattct gaaacaaaaa acgtaactca caaagagttg
    19381 tttttgcgtt ttttcttgaa tgattaggtc tgaatctttt gctcacaacc ttattcatgg
    19441 tgccccggct tgagctgata gatttttgaa ggacggccga ttcctacagg acgttcaccg
    19501 atctcatcga aatattggag catagctttc ttaaaattgg aatgatcgat cgttttataa
    19561 tcgatcccta agaatttagc aaatactttt cgagcttccg taatcgtgaa atctttacct
    19621 aaaacttgta gaacttgcgg ctcgtgttcc attttattga ctacacggtt gaatgctttc
    19681 aagatgattt gactatggtc aaaagccaaa gtatctttgc cagaagactc tccagtgact
    19741 aaatcaagcg agatagccac ttctccgcta gataaatgga tcttgttact atgacgttct
    19801 agtgtgaacc agcgtacttc cttggcatca tctccggcga ttagcggttc ttctccaata
    19861 aaggctaagt agctgactgt gacgacccag cctcgcggat ctcgattagg cgtactgaat
    19921 gtatgcagtt gctcaatatt ttcttttgaa ataaccacac cagtttcttc ctttgtttct
    19981 cttaagacgc tttctccagt tgactcatcc ttttggacaa agccaccagg caatgcccat
    20041 gaatttcgat aaggatgccc ttttctttgg atcaacaaaa ctttcagctg atcttcttct
    20101 ttgttatagc acatcagtac gatatcgaca gtcaaagagg gcttgtcata ttctggacgt
    20161 tcctgttttt gataccagtt aagaaattct tcttcacttg ctttatgttc ataatataat
    20221 ttttcttctt ctttagaagc aaaacggttc atctgaccac ctccattgac agtaaaagta
    20281 ctttttacct tattgtagca gactctgaaa aaaaaccaag gatttacttg aaaaatctag
    20341 gttatcattt aaatatgaaa aataaatgga agtatgagat tgaataaaaa gaaaagcaga
    20401 taaaacggca atactttata ccgctcttca cgaatggcaa agaagccaca aataacgata
    20461 tatacgtcaa tgataaaacg caaaatccaa gctgctgtca taaaactgtt caaatgccca
    20521 tggaggttaa aataaccata catacgaata taaaaatagc tgaatattaa atgtgctaat
    20581 gaaagaaaag caatactaag tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc
    20641 ccttactaag atagatagtc tttattatac cacgtgtgag gggagaaata aaaagccgtt
    20701 ttttagtaga tatgaaacaa aacaaaaacc cgcatttatt caacctattc atccttttaa
    20761 aggatagatt gaacgtagat agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc
    20821 taacagcatg ctttttttat ggtatactag caaggaaacg aattcgcatc atgcgataaa
    20881 ggaggaagag ccccgtggca gaaaaggaaa ctttttatat cactacgcca atctattatc
    20941 caagcggcaa acttcatatc ggcaactcgt atactacgat tgcatgtgac gcaattgctc
    21001 gttataaacg gttgatgggc tttgatgtgt tttatctcac tggagtggat gaacatggtc
    21061 aaaaaatcga aacaaaagca gaagaactag gtgtaaaacc gcaagaatat gtagataaaa
    21121 tggcggctga tgtcaagaaa ttatggaaaa cgcttgatat cagttatgat aaattcattc
    21181 gcacgacaga tgattaccat aaagcagctg ttcaaaaaat atttgatcgt ttattagaac
    21241 agggagatat ttacttaggt gaatatgaag gctggtactc tgtttctgac gaagaattct
    21301 ttacagaaac acaacttgca gaagtgtaca aagatgacga aggtaacgtg atcggtggta
    21361 aagcgccaag tggacacgaa gtagaattgg taaaagaaga atcatacttc ttccgcatga
    21421 gcaaatacgc agaccgtcta ttggaatact atgaagaaca tcccgaattc atccagccag
    21481 aatctcgtaa aaacgaaatg atcaacaact ttatcaaacc aggattagag gatcttgcag
    21541 tttcacgaac aacttttact tggggagtac cagtcaagag tgatccgaaa catgtggtgt
    21601 atgtatggat cgatgcatta tccaattata ttactgcact aggatatggc tccgaagatg
    21661 aaagtctatt cgaaaaatac tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc
    21721 gctttcatac gatctattgg ccaatcatgc tgatggccct tgatttgcct ctacctaaaa
    21781 aagtatttgg gcatggctgg ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa
    21841 atgtcgttta tcctgaaatg cttgtggaac gttatggttt agatgcactc cgttattatt
    21901 tgcttcgtgc tgttccgttt gggtcagatg gtgtctttac gccagaagat tttgtttccc
    21961 gtgtcaatta tgatctggca aatgatctag ggaacttatt gaatcgaacg attgctatga
    22021 tcaacaaata ttgtgatgga ctcgttcctg actatgcctc acttgtcact ccatttgata
    22081 gtgaactgtc tacgacggct gctaacgttg tcggacgtta ccatgatgca atggaaaaaa
    22141 tggaattcaa tacagcattg gctgaaatct gggtgctgat ttctcgtgca aacaaataca
    22201 ttgatgaaac agaaccatgg gtattagcaa aagacgaaga aaagaaagct gaattgaata
    22261 gtgtcatgat ccatttagct gaatctttgc gtatcgcagc gatattactt cagccaatca
    22321 tgacagaaac gccagagaag atcttcaatc aactaggatt ggactctgaa acaatgaatc
    22381 ttgaaggatt acactttggc gaatttcctt caggaacaaa agtagttgca aaaggtacac
    22441 caatcttccc acgtttagat atggaagaag aagttgcttt tattcaagaa aaaatgtctg
    22501 aaggtactca aacaaacgaa gatacagtca aatgggatcc agaagaaaca gaacttgttt
    22561 caacgaaaga aaaacaaatc aaatttgatg tatttgaaaa agtagaacta aaagtagcag
    22621 aagtcatcaa ctgtcaaaaa gtagaagggg cagataagtt gctgcaattc cgactagatg
    22681 ctggagacag ccaagaccgt caaatcttgt caggaatcgc tgaattttat cctgatccaa
    22741 gcgaattgat cgggaaaaaa gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg
    22801 gacaaatcag tcaaggaatg atcctttctg cagaagcacc agatggctct ttacaagtaa
    22861 tcgaagcacc aaaatccatg ccaaatggct cagaaatcgc atagaaattc aaagggggta
    22921 tgacactagt tgtgtcatac cccctttttc cgaaatttga gaagcaattt tcgtaaattc
    22981 cttcttatta cttgaagcca atcatataat acagaaacag agaactatcc aaaataaatc
    23041 ctagaaaaag aagtacgagc ttcgctgatt tttgttaatt tagaataata ctaataggaa
    23101 tcgtaaaaac agaaaaaaag cataatgata aagggtcggg cagattttaa ttgattcatc
    23161 tggtactctt ttttattgtc cttacattca taaagtttga atgcaatgac tttattacat
    23221 ctatatttat atctaacagt agcgttgatg tgttaattat aagttctatc actttatgtt
    23281 caattagttt gtgctcttat atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc
    23341 tttgacttcg atgattgttt tctttatttt gtaaaaatcc tatcacttga ttttatggta
    23401 accttttatt tttgtcttca aacggcttgg taagagagca gtaagcagtg caaatgggat
    23461 cgaaaagaca taccagaaaa tcgtacgtcc gataccataa tgttgataga gctttgtcaa
    23521 catcacgtgt aacaagatca accctaaaag caaggagcca gctataaacc aatgattatc
    23581 ccaatgcatg acttcactaa gtgccatcac tccaaaagga tagaaataaa aatagatccc
    23641 gactaaggtc atccgccagt tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc
    23701 tgaaagcaaa ataccgatag gcggaaaaag cccaaccaaa aaggaataca acatccccca
    23761 caagatcatc acaggacctt tgatcaatgc aacgattgcg atgaacaaag tcagcaatat
    23821 tagtttttgt gataacgttg caacagtcat taatccaaat aaaattaatg tgaaccaatg
    23881 ccaatgggac tgctgttgtt ctttacggga aaaatcgacc cggttggata aataatctgc
    23941 taatttcgga taagaacgct taaaatccat aaactgttcc tccttcgtgg aaaaggtagg
    24001 tttagtatac gaaaaataaa aaagaaatac atccgtctta agagggagtt tttcactgaa
    24061 agtggagaaa aaagaatggt aaattaaaat attctcagtt atactgaaaa ttataccaaa
    24121 tttcaaagaa gaaagaggat gggaatgaaa ggcaaactaa aacgagaaat caatctgttt
    24181 ggcgcattag ctaccgttat gggaaccgtg atcggtgcag gcgtcttctt caaaactgct
    24241 gctgttactg ctagtactca gtctacaagt ttgacattgc tagcttggct tttgggtggt
    24301 ttcttaacga tctgtgctgg attgacagtg gctgaattag ctacagcaat cccggaaact
    24361 ggtggagctg taaaatatat tgaagctgcg tatggaaaat tgcccagctt tttattagga
    24421 tgggcgcaaa gcttgattta ctttcctgct aatattgctg cattatcgat tatctttgcg
    24481 acacaattga cgaacttatt acaattatct accgattatc ttttgttgat tgcagtgatc
    24541 accgctgttt ctgtgacagg gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa
    24601 tcagtgacgt tgattgttaa attaatacca attgctgtga ttgtcatatg gggattattg
    24661 accccaggac aaggaactgt ccagttattt ccaatcgaag ctggaaaaga tgttactttt
    24721 gtcgaaggat tgagcagtgc gttgttagca acgttgtttg cttatgacgg ctggttaggt
    24781 gtcggtgcaa tggcagggga aatgaaacgg ccggaaaaag atttgcctaa agcaattatt
    24841 cttggtttga gttttgtaac agtcgtttat ctattgatca attttgtttt tttgaaaact
    24901 ttgccgattg atcatcttgc cggtaatttg aatgcagctt ctgaagcatc agacgtcatt
    24961 tttggaggaa tcggtggaaa gctggtcact attgggatct tgatctcggt atacggtgca
    25021 ttgaacggtt atacactaac tgggatccgc gtgccttatg cgatggcttt agaagacgag
    25081 ctacctttca gtaaacagct gacaaagctt tcaaagaagt ttacggttcc ttatgttcct
    25141 gcggttttcc agttagcagt agcttgtatt atgatgagcc ttggttcttt cgacttttta
    25201 acagacatgt tgatttttgt gatgtggcta ttcagtttac tgatttttat cggtgtcttt
    25261 attttaagaa aaaaagcgcc ggaattgcca cgtccatata aagtaccact ttatccaatc
    25321 gttccgatta ttgcgattct cggtgctatt ttcattttag gtatgacgat gatgacgcaa
    25381 acgaaattag cattgatcgg tataggtgtt accttgatag gtatcccagt ctattatcaa
    25441 aaaaagaaaa aacgctcaga agagtaaaat atttcaacaa cagacagaaa aagtacgaac
    25501 taggtcctct agatcttcgt gctttttcta tttcaataaa acaagttctt ctattttgat
    25561 ttttgctact tctgcaagta gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa
    25621 catgatattt gattctcata cccatttaaa tgctgaacag ttcaatgaag atattccaga
    25681 aacgattcag cgagcacaag aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac
    25741 gccaacgatt gaaaagtctt tgcaattaag ccatgactat ccaaatatat acagtattat
    25801 cggctggcat ccgacagagg ctggcagtta tacgaaagaa atcgaaaata aattacaaga
    25861 acaattgact ttaccaaagg ttgtagcatt aggggaaatt ggcttggatt atcattggat
    25921 ggaagacccg aaagaagtcc aagataaagt atttcgcaga cagattgcta tcgcaaaaga
    25981 aatgaaccta ccaatcagta ttcatactag agaagcactt gaagacacgt atcaaatcct
    26041 aaaagaagaa gatgttcgag atatcggtgg aatcatgcat agtttcagtg gtgattatga
    26101 atgggcaaaa cgatttcttg acttagggat gcatatttca tttagcggtg tcgtcacttt
    26161 caaaaaagca ttagacgtcc aagaggctgc agcaaatgta ccaatcgaac gattacttgt
    26221 ggaaacagat gccccttatc tggcaccagt tccttttaga ggaaagcgaa atgaaccagg
    26281 ctatactcgt tatacagtag aaaagattgc tgaacttcgg aatcttccga cagaagaagt
    26341 ggctgcccaa acatggacaa atgcgcaccg attatttggt ctaagcgaac atggataaga
    26401 tgaagatcca agagattata gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag
    26461 tagttgatgc agatacgata gaaacaatag ggtctgcaat caatgaagag atcctgatgc
    26521 aaattgaaca tgcccaagag actcgtggag tgatcgtttt taccgatcct gatttttcag
    26581 gagaaaaaat aagaaaaatc attatggaag ctgttcctca ggcaaaacat gcttttttgc
    26641 cgcgcacaca agctaaacca aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg
    26701 ctgctatttt agaagcattg aaaaaggtcg tgacgccaga catttcccaa acggctgttt
    26761 ctgaaatcac tagacaagac cttttagact atggtttaat agctggggca caagcaaaag
    26821 aaaaacgaga aaaactaggt gacgagttgc gcatcggtta cacgaacggc aaacaattgg
    26881 aaaaacgttt gaagatgttt cgtattacgc cgaaagaact cgcccaagca atgaaaatag
    26941 tggaggaaca acatgagtga atatcgtgaa attgcgacac cctctagaac aaaagagatt
    27001 ttattgaaac atggtttttc atttaaaaaa agtttaggac aaaattttct gactgaacca
    27061 aatattttaa ggaaaatcgt agaaactgcg caaatagatg aacatacgaa cgtgatcgaa
    27121 gttggaccag gaatcggtgc attgacggaa caattagcca agcatgcaaa acaagtcgta
    27181 gcatttgaga tcgatgatcg tttgatccct gtattggctg atacgatgca accctatgac
    27241 aatgtgacta ttgtacatca agatatttta aagaccgatt tgtcaacagc tgttagagaa
    27301 acatttcatg aggagcttcc gttaaaagtc gtagcgaatt taccttacta tattacgaca
    27361 ccgatcatga tgcattttct tgaatcagat ttggttgtgg atgaactagt cgtcatgatg
    27421 caaaaagaag tagctgatcg tatctctgct gaaccaggaa cgaaagctta tggttcatta
    27481 tcgattgctg ttcaatatta tatggaagcc agtttagcat tcatcgtacc aaaaacagta
    27541 tttgtccctc agccaaatgt agattcagcg attttgaaac tgacaagaag agatataccg
    27601 gcagtcgaag tgacagacga aaaagcattt ttccgattga caaaagcggc atttcagcaa
    27661 agaagaaaaa cgctttggaa taacttgcag cattcgtatg gcaaagatga ccagacaaaa
    27721 gcatggttag caaaaagctt ggaaacagct ggaatcgatc caaaacgtcg tggggagact
    27781 ttgtcattac aagaattcgc cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa
    27841 aagaggttgt gacagaagtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa
    27901 acaaaaatag ttcctcatgt ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt
    27961 tgaacaccgt ttaagaaaga ggttgtgggc ctgccgttta actctaagtc acaaggaaca
    28021 atttcaaaaa acagcttctc atgtttttga gttagttggt cttattactt gatgcaagct
    28081 tttgaccact attatgccga acaaggagat gtgacacaaa ttttgtcaca tctcctattt
    28141 ttgtttcttg atcaaaaaat tcttatactt gatactcaca ctcattttgt atggatttag
    28201 aaaagaatat gtgtcttgac agttattagt aagtgagtta tactaagtcg acatttatgt
    28261 gtcaagacat attaggagag agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt
    28321 tctgctttat tgattgggat gggggtcatt atcccaatgg tcatgcctaa aatcatgatt
    28381 ccaccagctt cctttacttt ggcgtctcat gtgccattat ttattgcgat gttttttaca
    28441 ccaggagttg cagtggctgt tgcgctaggt acgacatttg gtttcttttt aacaacgccg
    28501 gttatcatcg cattgcgagc attgtcacat cttgtgtttg cgttgattgg ggcttggtac
    28561 ttgcaaaagc atccgagtat cgttttaaaa aatggacagt ttacttttgc taattggcgt
    28621 ttccaagggt tcaattttgt gatcggtgtg attcattctt tagcagaaat gctagtagtc
    28681 agcatctttt actttattgg taatatgcct gaaacatatt attctcaagg atatttttat
    28741 acagtattca tcttgatggg gattggtggg cttatccaca gtttagttga ttataatatt
    28801 gcttatttta tagcaggaac actcagcaaa tattttgata tccccgtatt tacggcagca
    28861 aaaaaagaaa atcggaaaga gtcgattcaa gggtacgcga agaaaacgac atattagaca
    28921 tgatctctca aaaaaaaaac tctcatctag taatggataa taaatcctct aagatgagag
    28981 ttttttgtcc taaaaagagc tgcaccaaca tcttctggca cagctctaac agggggcaat
    29041 ttatggaagt ttgatgactt gttctgtttc tttatcaaag aagtggcctt tgctgatgtt
    29101 gaaggcaagg tcgactattt cgcctggttt atggaaatct cttgcatcta cttttgagat
    29161 gaactcagta gaaccagttt ttgtgtaaag catcgtttca gctccaagta attcggaaac
    29221 gacaacttct gatttcacta cggcttcagg tgaagcttca agggctactt gttcactgtg
    29281 gatgtcttct ggacgaatac cgaagataac ttcttttcct tcataacctt tttcaactaa
    29341 tactttgttt tttccttcag gaatacgaag cttaagccca tgtccatctg tgataacacc
    29401 atttttcaat gtcacattaa agaagttcat ggcaggagat ccaataaagc cggcaacgaa
    29461 catattgacg ggtgtatcat aaacttcttt cggtgtaccg atttgttgga taaatccgtc
    29521 tttcatgata acgattcggt cagccatagt cattgcttct gtctgatcgt gagtaacata
    29581 gatggtcgtt gtttcaagac gctggtgaag tttggcaatc tcagcacgca tggctacacg
    29641 taatttagca tctaaatttg ataatggctc atccattaag aagacttttg catcccgaac
    29701 gattgcccgt cccaatgcca cacgttggcg ttgtccacca gataaagcag ctggttttcg
    29761 tttcaagtat tctgtcagac ctaagatctc tgctgcattg tccacacgtt ttttgatgtc
    29821 tgctttatca tatttacgta atttcaagcc aaaagccata ttatcaaaaa cagtcatatg
    29881 aggatacaat gcgtagtttt ggaaaaccat ggcaatatca cggtctttag gtgccacgtc
    29941 attcatgact tttccgccga tatttagttc tccttcagag atatcttcca gtcccgcgat
    30001 cattcgcaaa gtagttgact ttccacaacc ggaaggacca acaaaaacga taaattcacg
    30061 gtctttgatt gatagattaa aatcagttac agaataattt tctgcattgt catatttttt
    30121 atatacgttt ttcagagcca tttctaccat aatacgttcc acactttctt ttttagtttt
    30181 attaactatt taaagtataa atgaaagcgt taactaactt caatgtcatg ttgaacaaga
    30241 attttttctt ttttttgtca atgtgctagt tttaagcttt aataaatatc cataagaaat
    30301 aaaaactgat acactggagt aaataagtat gttaacaata tcaaaaggag tgtctcttat
    30361 gaaaatcgct ttgatcgctc atgaccgaaa aaaacctatg atggtgaaat tagcaacagc
    30421 gtataaaaca atattggaaa aacatgaact ttttgctacc gggacgactg ggcagaggat
    30481 cacggaagca accggcttgc cgatccatcg gtttaaatcc ggaccgttag gcggggatca
    30541 gcaaattgga gccatgattt cggaggatca gttagatttg gtgatttttc ttcgagaccc
    30601 gctggctgca cagccccatg aaccagatgt aacagcactt atccgtttat ctgatgtcta
    30661 tgagattccg ttagctacga atatcggaac agctgaggtg ctactaagag gactggaagc
    30721 cggcttccta gattttcgaa gaatcgtcca tgaaatagac aagcatccac tttcattttg
    30781 atggataagc gtatggaaaa ataacgttta atgttcgcct atatgtagct ataaaagcta
    30841 aacaaactga taatatgcga tataatacga acgtattgga taatataaac aataatatta
    30901 gtttttcgta tttgcagtaa atgtgaaaat atgctatagt ttctgtggga aaagatccca
    30961 aaaataatac agagtagaaa ataaagcgtt aagtgctaag aggatgggat gttgcctctt
    31021 ggacgaagga cgtaatgttc gcggttttat tttcgcattc gctgcatgat acgatgtagc
    31081 agctcttaaa ggagatgcta aaaatgaaga aacaccggtt agacgcacgt atgattgcta
    31141 ttatgggctt attgattgca ttgatggtca ctttgtcccg actggtagca atcgagaccc
    31201 catttattaa aatcagtgtc acatttattc cacaagtgat catgggcatt ctttttggtc
    31261 ctttttggtc agggataggc gcagttttag ctgatttagt aggaatggcc ctcttctcga
    31321 aatctgcatt ctttattgga tttacattga atgcatttat tgaaggagca atctacggat
    31381 tctttttcta tcgaaaagag atcacttgga aaaatgccat tctggctacg cttagtgtca
    31441 cgctgatcat caacttattt ttgacaccat tatggcttgc gttgatgtac catgtgccat
    31501 tgttcagctg ggtcgtttgg gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc
    31561 aatcaattgc catttattac gtaggacgta gtattcctta caaaaaaatt ttaagaagtt
    31621 tagcgatcca cgctaaataa aaaacaagca gtcttcccat cccagaagac tgcttgtttt
    31681 tattgtgctg tgaattgtgc ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc
    31741 tcatcatggg tgcccatttc gacaatagct ccctgatcca tcactaatat ctggtccgca
    31801 tttttgattg tggacaggcg atgggcaatc acaaaactgg tttttccttc catcattgct
    31861 aagaaggctt gttggatttt tttctctgtc aatgtatcga ctgagctggt agcttcatct
    31921 aagatcaaca tagggggatt actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt
    31981 ccatctgata tctttaaccc ttgaccgcca atcttagtat ctaatttttc cggcaaacgt
    32041 tctacaaatt catacatata agaggctttt agggcatcat tgatttctgc gtcacttgct
    32101 tccggattcc caaaacgaag attttctcgc aatgtactgt caaatagcca agtatcttgt
    32161 aacaccattc caaaactttt acgtaatgta tcacgttgga tagagcggat gtcatggtca
    32221 tcgactgtaa ttgttccttt tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg
    32281 gattttccgg cccctgtttt tcctactata gcaatggttt gacctggata agcagtaaaa
    32341 ttgaaatctt cgatcaaacg ctgcttgggg tcataagaaa aagaaacatg atcaaaagag
    32401 acttttcctt tgatattttt gggttccaag gcataggcat ctggcaaatc aggcttttct
    32461 tctggctgat cgatcaaatc aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt
    32521 gtcgtgattc cagaaagttc gataaatggt ttagaaaact gacttgagta gatcgtaaag
    32581 ctggaaatca caccaacagt gattgacgaa ccactcttca atgccaatag gccgccaact
    32641 aaaccaatcg ataaataagc aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa
    32701 gaagaaaatt gtgcccgttg tcctttttca tatagatcag catttagcag ttcaaattgc
    32761 tgttgactag cttcttcttg ctgaaaagct ttcacgattt tctggttacc gattcgttcg
    32821 gtcacaaagc ccgagatatc cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa
    32881 gcacgagcaa cgatccaact cacaaggaaa ataattggtg tagaacaaag aacgaccaat
    32941 gtcagaagtg gacttagacg catcataaaa ataaacgaaa gaatgatgat tgccactcca
    33001 gaaaataatt gattgaaggc cgcagaaatt gcaatggaaa tattgtccat atcatttgta
    33061 aatcgactga cgatatttcc gtgggagttt tgatcataat aacgaagtgg caatcgattc
    33121 agatgagcaa atgcatcttt tcttaatttt gcaacggata agtaagctaa ccgattgctt
    33181 aacacttgga tgagccattg actgactacg ttgattaaaa cgataccacc gaatagaata
    33241 agtaaatgga acagtcgatt gaagcggacc tgtccttctc caatcatttg atcgatactt
    33301 tgaccaattt gtaacgtcat cacaactgtt gcaatcccat tgatgattcc tagtatcaca
    33361 gctagccaaa tgtcttttgc ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc
    33421 aaggataatt tttttacttt cataactatt cttcctcctg tgatgcgacg atttcctgat
    33481 attctgctga ttgagcaagt aattcttcat gggtaccctg agcagcaatc ttaccttgat
    33541 ccatcactaa gatatgatgt gcttcttgaa tagagcggac acgttgggag ataataatca
    33601 ctgtactgcc aagataagtc tgtagcgcct tacgcaaatc caagtctgtt tgataatcca
    33661 acgcacttaa agagtcatct aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa
    33721 tcgtcagtcg ctgtttttgt ccgccagaga aatttttccc attttccatg acttttgtat
    33781 ctaaaccatt tggaagctga cggacaaatt cttctgcctg agcgattttc agagcttccc
    33841 aacattcttt ttctgtggcg tctttcttac cccattgaag gttttcacga atagtcccgg
    33901 taaatagtac agaagtttgg ggaacaagag caatttgctg acggagatga tccagctgcc
    33961 aagaacgaac atccattccg tccattataa ctgtaccgga actgacatca tagaaacgcg
    34021 gaatcaattg tgtcaaagtc gttttcccgc ttccggttgg accaatgatt ccaaggactg
    34081 tatttcgctt cagctgaaat gtaatatttt ggagagaaag accagaatca ggagtataac
    34141 ggaagtcaac atggtcaaaa gcgataattg cttcagaaga aaaatcggga gtagtactga
    34201 cctcagcttc aagaatcgaa ttttctgtgt ctagcacctc tttcacccga tttcctgaag
    34261 cttcggcacg tgtgaagatc acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt
    34321 gattcatata attgatcaaa gcaagaactt gtccttgttg caagctacca atatttactt
    34381 tgatcccgcc aactgtcagc aaacagatga tcccgacatt catgatcaag gttgtagcag
    34441 gtgataatag ggcagagata ttggaaacac gtaaataatt gttcgctaga tcatttgtgc
    34501 ttttatcgaa cttctctgtt tctttttttg tctgcgcaaa tgcgcggatg acacgaacac
    34561 cgctaagatt ctgactaacg tgttcattga gctgatctag tttttcttgc actttttggt
    34621 agagaggaac ggtttttttg atgataaaaa ataaaatgat tgagaaaata ggcaataatg
    34681 ctaagaaaaa cagacctact tcccagtcga tcacaaaagc catgaccact gacccgatac
    34741 ttaaaaacgg tgctctgatc aataaacgaa tgaccattgc taaagctagc tgaagctgat
    34801 tgatgtcgtt tgtcattcgg gtgatcaagg tatctgttcc aaaatgattc aactcgttgt
    34861 gagagaaagt attgattttt ttgattagct gattacgtaa ctctgtacca aatccttgtg
    34921 aagctactga agcatagtat tggcaaatga tagcacaaat cagaccaatc acagacatag
    34981 cgaccatcca taagcccatt tcaatgattt ttcctcggtc attcattttt agtccattat
    35041 cgatcaagga agccatcatt aaaggcagga caagttcgaa gacagcttca agaaatttga
    35101 agaacgggcc caaaatgatt tgcttacgat agtcttttgc ataacgtaat aactgaatca
    35161 tatgattcct cctattattt atttgtccac aattgaaaaa acaagtacta ccacattgta
    35221 gcttaggaaa tttagaatga aaagagaaca agccttttca taaatgaaag ttttattatg
    35281 aagaagcttt agaaattgaa tagactattt tttttaaaaa aaagaaaatg agaaaacatg
    35341 ccagtctctt cattcatagg aacgtctatt ttacttaatt tttctataaa tgaattctgc
    35401 ataaaaaata tatcaagtaa atgtttatag atgaaactct atttacttct tttttaattt
    35461 gttcttgcaa tcttttttgc tacgtaattt ttgtaaaaaa gtgggttgag agaaatctaa
    35521 ttgattttca aagaaggagt atgaaaatga gaaaaatatt tattcgttta ttgtgggtac
    35581 tatcttagag gaataaataa tagtggagaa aactatggtt tggcgcagat tctatcaggt
    35641 ttgatggaac gaagacaacc tcccgacatc aagtcaaaag ctacttgaga ttttgatctg
    35701 aaaataaaaa aagaaaacaa ggtaggaaca tcaatgtttc taccttgttt tcgtaatcaa
    35761 tgggaaaaaa ctaagagtgg tgtacgtaat caataaaaag gatggtgaca acagtacctc
    35821 tttactctct gaaagcatca taactcgttt ttcctcatta tgccaataaa ttgttgttat
    35881 accacatata aggatttgta atagctaaaa acagacagga aagttgaaat ctgtgaatct
    35941 tattttgtat ggaaggattt atagaacgca aaaatcaaga taaagatgaa taaaaatcct
    36001 gtttcggcta tatattgcgt atctacgcca ataaaacgag tttgttctgt catagccata
    36061 taaagcaaag gcaaaaatac aactacagcg agataagatc cgatgatttc tcgaaaagtt
    36121 ttccaggaag tatgtttcct gtttttatat ttatccgatt tcattgaata gtcatagcct
    36181 ccttattctt tcatatgttg aaattccagt ataaaaactg gaaggaactg tacgtttttt
    36241 agaataaaaa ttataaggga taagtaggct gctgtctata ataagatata tttttcaatt
    36301 ctttattaaa gattttattg acaaaatgaa tccgttttct atgatggagg aggggtgaat
    36361 aagttgttga agaaggaaat tttatgttac ttagagaacc agacagcctt tttcgattta
    36421 gaacatatga acgaggtctt tacagcacaa aggttagctg atcattttgg ggtaaaaaga
    36481 aacacagtca gtcattattt gaaccagtta aatgatgagc aactactagt caaaatcaat
    36541 tctcgaccag tgatttattt ccacaaagaa gcttttgaaa aacaattttt taagttacgc
    36601 acgaattttt atttttctat cgatgatctc aaagaggaac agcctttttt cgcgcaacca
    36661 aaagacttat tctcttttat gatcggatac gacgccagtt tgaaagaaag tatcgaacaa
    36721 atcaaaacag ctctttacta tccagatggc ggattaccac ttttgattac aggcgaaagt
    36781 ggcacaggta aaagttattt agttaatttg gtctatcagt attgtctgct tcatgatctg
    36841 ctagaagatt ctgcaccatt catcactttt aattgtgcac aatatgcaga taatcctgaa
    36901 cttttgacta gcaatttgtt tggacatgtt aagggagcgt acactggtgc agaagaaaat
    36961 aaaaaagggg cttttgaagc agcagatggt gggatcttgt ttttagacga ggtacatcgc
    37021 ttaacaccag aaggtcagga aaaactgttc acctatcttg atcaaggtgt gatttatcgt
    37081 atgggagaac ctaatttgtc acgccggatc aaaacacgtc tcttttttgc tacgactgaa
    37141 gagattacta gtcatttctt aacaactttc attcgaagaa tacctatccg aatcgaacta
    37201 cctgcactta atcagcgtag tcgtaatgaa cggctagaac tgatttactc tttttttctc
    37261 gaagaacaaa gaaaaatggg gagccctttg tcagtcagtg gaccagcttt gtcactttta
    37321 acaagtcaga aattatccgg aaacatagga gagctgaaaa atatcgtcaa agtttcagca
    37381 gcaaaagcat atgctgaaca gcgtgaacag cctgaaatcc atgtcacgat ccatcatttg
    37441 caaaaagaac tacttgcgca gcctattact aaaaatacag cacagcaagg aatctatatt
    37501 acgcaagacc agaccttaga gcaattaatg gagaagcaac agcccgaaca gcagcgaatc
    37561 gttaaaagtt ttgagcaaat cctaattgat ttcaagaaaa ataactgtct gctatattct
    37621 tgcgaaggaa aattaaagca agaagtgatt caattatttg attttttgct gtttgagacc
    37681 agtagacaag aaaaacacga gctgctggtt tattggacac aatctatccg agagaccttt
    37741 cgacaaatgg aaactgccta tcaaatcaaa ttcgatggaa acagtgtcta tgccttgagt
    37801 tactatctct atcagcgaag cactatcaaa tggttaccgg aagaacatga tattatccaa
    37861 ctaatcaaag aattagaaca acaagtttct cagtcttatc catctagtta tcactatgtc
    37921 caacgaattc tggaattaag taagatcaaa ttagatattg aagacacaag tatggatcgg
    37981 attttactga cgatctatct aaaaaaagcg aaatggtcaa aagaaaaacg actgccaaaa
    38041 gcgatgattg ctgctcacgg ttatgcaact gctagcagta tggcaaatgt agtaaatcgt
    38101 ttattacaag aggatctatt tgaatcattt gatatgccac tagatgtcac accacagcag
    38161 attgcttcag aaatccttga ttattgcgaa aacagcgatg tttcaaatgg attgatcata
    38221 ttagtagaca tgggatctct gaaagaaatc catcagtttt ttaaaaaaca gttatccgta
    38281 ccgcttttga ttttgaataa tgtgacgaca cctttagcaa tcacagtagg ggaatgcctg
    38341 caaaaaaatg tatcattaga agagatagca gaagagactg ttcgacagat ccagccagaa
    38401 tggcgactgc tctatccaga ggaaaacaaa cccaaagcac tcattactac ttgcttcact
    38461 ggaatcggta cggcggtcca cttaagtgaa ctgctagaaa aaagcttacc tacggcttgt
    38521 cagttgaaaa tcatcccata tgaatatcag caattaaaag ataagaaaaa cagtgaccca
    38581 ttattttcca tatacgaagt cgtaggaatg attggcacca cagatcctga tatcaccaat
    38641 atcgactatc tttcattaga agacctcatc tcgggggaaa agatgtctgt actagctgaa
    38701 tggttggaca ctactatgaa tgcttcagag aaggaaatat tcaatcagcg catcattcgt
    38761 aatttttcat tggaaaaagt actggattcc gtgactattc tagatacaga gaaggtgatg
    38821 ggagaaatcg atcattttat gagagaatta gaggtgcagt tgaatcgaag catcagcaat
    38881 cccaaaaaat tagcgttgta tgtccatgtg agctgtctca ttgaacgatt gatccgacaa
    38941 atgccaatcg aaacctatca aggattagag aaatggaagc agtgtcaaaa agaagggtta
    39001 tctgctatta aatctgcgtt tagtgtcatt gaagagcatt atagtgtcat gatccccaat
    39061 tctgagattg cctatatcta tgatattttg agtgaagata cagagtttct ctcgatagaa
    39121 gaagaatttt gaacataagc aagttgttgc cactaatttt ggcatgcaac ttgctttttt
    39181 agtatgtgta agtgaaaaga ggagatgaaa aagtggatag agaaattatc ttagcttccc
    39241 atggaaaatt tgcttcaggg atattagatt cattagagct gatctatggg aaaaatcatt
    39301 ctattactgt attcgattgc tatactgatg agaattttga tttagcggaa acagtaaggc
    39361 agctttttat gaggtacaaa gataaagaaa tcattgttct gacggatctt tttggcggaa
    39421 gtgtcaacaa cgaattctta caatatatca atcgggatca tgtctatctc gttgccggat
    39481 tgaatttgcc actattgatt gagctagtat ctcggttgga cgctgaagta gagacaactg
    39541 ttttgatcca gcaggtgcta actgattcaa aaaaaatgat ccagttttgt aacgagcggc
    39601 tcaatcagaa aatagaagaa gacgaatttt aggaggaaga aaaatgattg tattgacaag
    39661 agtcgaccat cggttattac acggacaagt agctttttca tggacacaaa caataggcgc
    39721 agactgtatt ttgattgcta atgatgatgt acctacgaat gagatacgaa agacaacgat
    39781 caaattagcc aagccacaag gagtcaaact agtgatcaaa tcaattgatg actcgattgc
    39841 cgcgttgaaa agcggggtga cagataaata taagttattc atcgtagtgg aatcgatcga
    39901 ggatgcatat aagctggcag aagcgtatcc agaaatcaaa accatcaact taggagggat
    39961 caaggcaaaa cctggtactc gcagcatcgg aaaagcggta aatattttac cagaagagga
    40021 ggaacgcttg aaacgattaa ttgacaaaca aatagaagta gaaatccgtc aagtaccagc
    40081 agataaaaaa gtggatgtaa gagaagtttt gtaagaaagg aagaacctca atgattgtac
    40141 aagcagtctt attaggaatt gttgcatttg tcgcacaatc agaatatgca ctagggacat
    40201 cgttgctttc tcgtccgatt gtgacggggc tgtttacagg aatcgtgtta ggtgatgtaa
    40261 aaactgggat cattatgggc gcaactttag agttagcatt tatcggatcg ttctcagtag
    40321 gtgcttcgat tccacctgat gttgtgaccg gtgggatttt agggacggct tttgctatta
    40381 cagctggagc cggtacagaa accgcgcttt tactaggttt gccgattgct acgttgacat
    40441 tgatattgaa aaatatttac ttagggcttt tgattccaat tatgaaccat aaagcagaca
    40501 attatgcaga agaaggaaat tataaaggag tcgagcatat gcacttgctt gccggtatcg
    40561 gtctttcctt gatgcttggt gttgtggtaa tggtttctta tatggtggga agcaatacga
    40621 tcggaaatct attaaatatg attcctgact tcgttcaaaa ggggttgtca gtagctacag
    40681 ggttgattcc agctcttggt tttgcgatgc ttgctcgttt gctgatcaac aaacaagttg
    40741 caccatattt tttcttaggt tttgctatag cttcttactt agagattcct gtgaccggag
    40801 tagctatttt tggtgcgatt ttagcagttg tggtagtcaa tattatgaat gtccgtcaac
    40861 taccacttca aacaactgca ggggaggtag aagaagatga agaattctga gatcaaaaac
    40921 agcaaaataa cgaaaaaaga gctaaatcac gtattttggc gttcttttca aatggagttt
    40981 tcttggaatt atgaacgtca gatgaatatg gcttatgttt atgcaatgat tccgattttg
    41041 aaaaagttat atcagtcgaa agaagaaatg gcagcagctt tgaaacggca tctagagttt
    41101 tttaacacga ccccacatat cgttacactg attttaggaa tcacggctgc aatggaggaa
    41161 gaaaataagg atgatcctga atttgatgtg actgcgatcg atagtatcaa aacatctttg
    41221 atgggacctc ttgcagggtt aggagattct ttcttttggg gaactttgcg attgattgcc
    41281 acaggtgtgg gtacctcttt agccatgcaa ggaaatattt taggccctat cttgttcatt
    41341 ttgattttca atattccgca cattctgttt cgctatctag caacaggttg gggatatcgc
    41401 ttaggaacgg gttttttgaa aaagatccag gcaaacggta tgatggaaag tttgacatta
    41461 ggcgcgtcta ttattggact gatggtcgtt ggagcaatga ctgccacgat gatcaccatc
    41521 aatattcctt taaaaatcgg ttcaggagaa aatgctgtga ctgttcaaag tatttttgat
    41581 gatatcgttc caaatatctt atgtcttgga gcatttggca tagtgtttta tctattgaag
    41641 aaagaagtga aaccattaac gatcttgatc ggactagctt tctttggaat ttttggttcg
    41701 tggataggga ttttttaaaa aaggagcaga gaaaatgttt acgatgcaag attatatcta
    41761 tgaagaaaag gaagtactaa gtacgatctt gaaaaagaat gatttttcga caagagaaca
    41821 tatgaaaaaa acagtgaatt tactgatctt agcaacgggt tcatcttata acgcttgttt
    41881 agcagcgaag ccagcattgg aatcttatgg tgatcttact gtcgatattc aagaaccttt
    41941 ttattttttc aattatggaa aactttctcc ttcaatcgat actgtgattg ctgtgtcaca
    42001 aagcgggaaa agtgcttcta ctgttgaagc agtaaaaatg atacaaaaac aagggttacc
    42061 tgttgttgct atcacaaatg acgtgcaaag tccacttgct ttagaagctg cacaaattat
    42121 tgatttaggt gccggggtag aatcagtagg cttcgttaca aaaggctatt ctgcgactgt
    42181 tttacagtta ctactcttag ggataggaat tggaataagt aaaaataaaa tcagcaaaaa
    42241 aatagaacaa gactacatgc aacagttgcg caaaataatc aaccatttac cagcgattat
    42301 tcaaaaaacg gaagaatttt ttgatggata tcaaagttta tttcgtttag cacagcgttt
    42361 cgtcaccatt ggatatggtc caaactgggg aacagctaaa gaagcagaga cgaaattgac
    42421 tgaaacaatc cgcgttccgt cacaaggatt tgagttagaa gcttatatgc acggcccata
    42481 cctggaggca gatgcttcgc atttgctatt ctttattgaa ggggaaagtg taaataagga
    42541 acgttcgcaa aagttgcaaa gatatatgag tcggtacgtt ggagagactc tcaccattac
    42601 cacaaaaaaa gcaagaaacg agaaaacact tggattagca atagaatgtg atgaatatct
    42661 gtctgtatta gcgcttgtcg ttccgtttca actattcgct tataaaaccg cagtagctaa
    42721 aggtattgac ttgaacaaaa agatttttga agactttgat acagtcttga aaagtaaatt
    42781 ataagaggtg gaaatcatgc tgaaattcaa tgaagaagaa caaatcaaag caataaaagg
    42841 cgcactagct ttacgtccgc aagtagaaga aatcatcgat aaactttatg cggaaaaatt
    42901 tgacgctgtc tattatctag gaatcggtgg aacgtatgct tcctcgatgc aagctgtcac
    42961 ttatatgaat gggaaaagca acttgcctgt ctttgtacag catgctgcag aatattacac
    43021 tactggaaac aaaagattaa caaaagattc tatcgttatt ttgtcctctg ttaccggtac
    43081 aactcaagaa gtagtgaaag cagtagaaca aatcaagaaa gtaggagcga cactgattgg
    43141 atttatcgat aaagcgaaca gtaagttgtc tcagctatgc gattttgtgg tcacttatcc
    43201 agctcctgga acagaacaaa tcaaattttt tatggcagca gatcgattga tgtatttgaa
    43261 tggagaattc gaagcttatt cagaatacta tgaacagctc gaacgttatt taccaacagg
    43321 gttagtggaa gcagagaaaa aagcagatgc atttggctta tcatttgcag aaaaacatcg
    43381 acgtgatagt atgcattatt ttattggagc agggaatcaa tggggagctg tttattcata
    43441 tgccatgtgt tactgggaag aacaaagctg gcttccatcg aaatctatcc atgctgccga
    43501 gtttcttcac gggacgttag agatcgtaga agaaacaacc ccagtcacat tatttttagg
    43561 cgaagatgaa cagagagtgt tatctgaacg tgtggctaaa cttttgccaa aaatttgcag
    43621 caattataca ttgatagaca caaaagacta tccagtagaa ggtatcagtg aaaaatatcg
    43681 cggacgtgtt ttgtctttct tattgatgca tgtagtgaca caacgcatcg atgctcatgt
    43741 ggagcagttg aactgccatc cattagagat acgccgctac tatcgtcagt ttgattatta
    43801 ggatagactg gataaaaaga atataaaaaa taaagagagc agcgataact gctcccttta
    43861 tttttttaaa atattggcat caattacgtt ataaaacaca ttttttgcat tatctacagt
    43921 tcaacttata atctgtttaa tgggacatgt gggtgccaac cattttttgt tatgcaatag
    43981 tactatttag ttgttgcatg gatccgtagt aaaagtccaa gtacaacgag ttgcgccagt
    44041 ttacatagcg ttttgtatcc agaatgttga ctaaaacaaa agttcctatg tcaaccgtca
    44101 acagagacaa tctgtccgtt gcaggacctg acagatttag aaatgttcaa tctgtctctt
    44161 atgtatttta aatcaatact cactaggaga agtttcctaa cctcctaata gtaatgctgg
    44221 tagtaacatt gtcaaaactg cttcctctta ggaacaagca aagcataacg agtgtaaaaa
    44281 taaaatgtag actattgtaa catatcattg attatttcaa gtaaaaacga taaaagaagt
    44341 aaacaaacac ttaaaaacaa tggaaattag tcccaacttt aagaaatgta tactataatg
    44401 gaaattatca attatttatt tggaggaata tccatgaaac aaaaaatcgc agttcttggt
    44461 cctggatctt ggggcactgc tttagcacaa acattggcag aaaacgggca tgatgtccgt
    44521 atttggggaa atgtacctga acagattgat gaaatcaata agtatcatac aaatcagcat
    44581 tttttacctg atttgacgat cccagaatcg attatcggat ataaagaatt ggcagaagca
    44641 gtggataacg cagatgctat tttatttgtt gttccaacaa aagcgattcg ttcagtcgca
    44701 caggaactta ttggtaaaat gaatactaag ccaatcatta tacatgcaag taaaggacta
    44761 gaacaagata cgcataaacg aatctcagaa gttttggctg aagaaattcc agaagaaaaa
    44821 agacaggcaa ttgttgtatt atctggtcct agccatgctg aggaagtggc agtacatgat
    44881 atcacaacaa ttacagctgc aagtattgat caaaaagcag cagcctatgt acaaaaactg
    44941 ttcatgaatg attatttcag gatttataca aataatgatg tgattggcgt agaaactggt
    45001 gcagcactga aaaatatcat tgcaattggt gccggcgcta tccatggatt aggattcgga
    45061 gatgatgcaa aagcagcaat catgactcga ggattggcag aaatcagccg tcttggtgtc
    45121 gcgatgggtg ccaatcctct gacatttatc ggtttgagtg gtgtggggga cttgatcgtt
    45181 acttgtacaa gtgttcattc tcgcaattgg cgtgccggta acttgttagg acagggacat
    45241 aagctggaag aagttctaga aaacatggga atggtcgttg agggtgtggc aacgacaaaa
    45301 gcagcagtag agctagctca acagctaggt gttgagatgc caattactca aacaatctat
    45361 aatgttttgt ataatggaga agatatcaaa aaagcagcaa aagaaatcat gttacgtgac
    45421 ggaaaaatgg agaatgaatt ttctcttcgt taaatagatg atcaatagtt aatcgtc
//
LOCUS       DASNNU010000011        41113 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.011, whole genome shotgun sequence.
ACCESSION   DASNNU010000011 DASNNU010000000
VERSION     DASNNU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.011"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="U8116_000584"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="U8116_000584"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEN8433600.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="U8116_000585"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="U8116_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEN8433601.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="U8116_000586"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="U8116_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEN8433602.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="U8116_000587"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="U8116_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEN8433603.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="U8116_000588"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="U8116_000588"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEN8433604.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="U8116_000589"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="U8116_000589"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEN8433605.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="U8116_000590"
     CDS             complement(8557..9027)
                     /locus_tag="U8116_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEN8433606.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="U8116_000591"
     CDS             complement(9073..9498)
                     /locus_tag="U8116_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEN8433607.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="U8116_000592"
     CDS             complement(9565..9834)
                     /locus_tag="U8116_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEN8433608.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="U8116_000593"
     CDS             complement(9845..11440)
                     /locus_tag="U8116_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN8433609.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="U8116_000594"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="U8116_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEN8433610.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /locus_tag="U8116_000595"
     CDS             complement(14993..15553)
                     /locus_tag="U8116_000595"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEN8433611.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="U8116_000596"
     CDS             15906..16880
                     /locus_tag="U8116_000596"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN8433612.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="U8116_000597"
     CDS             17050..18450
                     /locus_tag="U8116_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEN8433613.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="U8116_000598"
     CDS             complement(18528..19250)
                     /locus_tag="U8116_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN8433614.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="U8116_000599"
     CDS             complement(19329..19835)
                     /locus_tag="U8116_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN8433615.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="U8116_000600"
     CDS             19958..21757
                     /locus_tag="U8116_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEN8433616.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21825..23300)
                     /locus_tag="U8116_000601"
     CDS             complement(21825..23300)
                     /locus_tag="U8116_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN8433617.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTVPESSATEESTTVPESSTTEESTTPAPTTP
                     STDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPY
                     VWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQ
                     GGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDFAVSM"
     gene            complement(23555..24064)
                     /gene="mreD"
                     /locus_tag="U8116_000602"
     CDS             complement(23555..24064)
                     /gene="mreD"
                     /locus_tag="U8116_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEN8433618.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(24068..24922)
                     /gene="mreC"
                     /locus_tag="U8116_000603"
     CDS             complement(24068..24922)
                     /gene="mreC"
                     /locus_tag="U8116_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEN8433619.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(25077..25715)
                     /locus_tag="U8116_000604"
     CDS             complement(25077..25715)
                     /locus_tag="U8116_000604"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEN8433620.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25716..26366)
                     /locus_tag="U8116_000605"
     CDS             complement(25716..26366)
                     /locus_tag="U8116_000605"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEN8433621.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26379..27278)
                     /gene="rsgA"
                     /locus_tag="U8116_000606"
     CDS             complement(26379..27278)
                     /gene="rsgA"
                     /locus_tag="U8116_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEN8433622.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27443..29512)
                     /gene="pknB"
                     /locus_tag="U8116_000607"
     CDS             complement(27443..29512)
                     /gene="pknB"
                     /locus_tag="U8116_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEN8433623.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29509..30249)
                     /locus_tag="U8116_000608"
     CDS             complement(29509..30249)
                     /locus_tag="U8116_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEN8433624.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30262..31620)
                     /gene="rsmB"
                     /locus_tag="U8116_000609"
     CDS             complement(30262..31620)
                     /gene="rsmB"
                     /locus_tag="U8116_000609"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEN8433625.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31620..32558)
                     /locus_tag="U8116_000610"
     CDS             complement(31620..32558)
                     /locus_tag="U8116_000610"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEN8433626.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32551..33042)
                     /gene="def"
                     /locus_tag="U8116_000611"
     CDS             complement(32551..33042)
                     /gene="def"
                     /locus_tag="U8116_000611"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN8433627.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(33057..35465)
                     /gene="priA"
                     /locus_tag="U8116_000612"
     CDS             complement(33057..35465)
                     /gene="priA"
                     /locus_tag="U8116_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEN8433628.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35718..36023)
                     /locus_tag="U8116_000613"
     CDS             complement(35718..36023)
                     /locus_tag="U8116_000613"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN8433629.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(36028..36642)
                     /gene="gmk"
                     /locus_tag="U8116_000614"
     CDS             complement(36028..36642)
                     /gene="gmk"
                     /locus_tag="U8116_000614"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEN8433630.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(37129..37413)
                     /locus_tag="U8116_000615"
     CDS             complement(37129..37413)
                     /locus_tag="U8116_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433631.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37431..38321)
                     /locus_tag="U8116_000616"
     CDS             complement(37431..38321)
                     /locus_tag="U8116_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEN8433632.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38341..38559)
                     /locus_tag="U8116_000617"
     CDS             complement(38341..38559)
                     /locus_tag="U8116_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEN8433633.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38529..40229)
                     /locus_tag="U8116_000618"
     CDS             complement(38529..40229)
                     /locus_tag="U8116_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN8433634.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40496..>41113
                     /locus_tag="U8116_000619"
     CDS             40496..>41113
                     /locus_tag="U8116_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433635.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKY"
BASE COUNT        11178 a         8875 c         7435 g        13625 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttgattaatc attcttacat gctgacagca
    21841 aagtcagggg caaaccattg aactgaacca acttttacgc tttcgcctgg ttgaggagca
    21901 tggatatatt gtcctccgcc taaagcaatc gctacgtggt aagttccgcc ttgtgacccc
    21961 cagaataaca agtctccagc ttttgcttga gaaactgaaa tttttgttcc agctgattct
    22021 tgaggaactg tccaaccacc gatgtcacga ccagttactt gcatgtaaac atagcgtgtg
    22081 aaccctgagc agtcaaagcc acttggatct ttaccgcccc aaacatatgg agtaccaata
    22141 tatttgtaag cttctgctac gattgctgca ccattcacac ttccagaagg agctggagca
    22201 ggtgttacag gttttggttg ttcaggtgtt ggtgttggcg ttggagccgg agtactactt
    22261 cctgtgccgt ttcctgtatc aacactttga tctgttgatg gtgttgtagg cgcaggtgtt
    22321 gttgattctt ctgttgttga agattcaggc actgttgttg attcttctgt tgctgaagat
    22381 tcaggcactg ttgttgattc ttctgttgct gaagattgtg tagctgatga tgattcttca
    22441 gtcattgaag attgtgtagc tgatgattct tctgttgctg agctttgtgc agtagatgaa
    22501 gcttgagtag cttgtgcttc agcctctgct tgttcgcgtg cttctttttc agctttttct
    22561 tgtgcagctt gttgacgagc ttgttctgct agacgttgtt gttcacgaat acgagcttgc
    22621 tctgcttcag cttctgcttt ttgacggttc aagtcagctt tcttgtcttc agcagttgct
    22681 tgttcagcag ctaatgaagt tttcagtacg ttcaaatcag cttgtttaga taaaagatcg
    22741 cctttttgag attccaaagc agcttggttt tcagctaatt cttttagttt agcatcgttt
    22801 tcagcttttt tgtcttctac agctttttta tcttgttttt gttgttccat caagtcgttg
    22861 tttgctttta ccatcgttgt cattgcttga acgcgtccaa tagcatctgc taatgaatca
    22921 gcattcaata cagcatcgat gtagtttgag cttgtgttgc ttacttgtgc ttcgcgtgct
    22981 tgtttttgga ttgtatcttc acgtttttca atacgttctt gtaaatctgc aatgtctttt
    23041 actaattgtg cagattcttg acgcaatgta tcttgttttg ctaataggtc ttgtgcttgt
    23101 gtgttgattt cagaaacttg gctttctaag gcatcgattt gtgattgtgc atctgcttgt
    23161 tgatttttca agtctgcaat cttttgatct tgctgttgta tctgagaatc aaaatcgtcc
    23221 gccgcggcag cgatcggtga tgctacggca gtcaaggtca ttgaacatac cattactgct
    23281 gatattaaac tctttttcac tcttcattcc tccgactggc ttaatttaat aaatatctaa
    23341 atatatttaa gtctttctta acgatataaa aattgtacca tagcagtgtg tcaacgatat
    23401 aatagttatg ttacaaaaat gtttcaaaac aacttttaaa aatgacaaaa ctgttcgttt
    23461 cagaaaatga tcatgaaaaa gccgcgttaa gtctagcatt tcttaagata acaacgattc
    23521 tttttcgctt tcgacctaaa aataaaaaaa gaaattattc atcactgaat aatttcttaa
    23581 agggaaaaat gaataataca aatataatca tgttcaacag cagtgtaggt cctaaaaacc
    23641 ttgtaataaa atacgtgtta tttactactg ctaatttaaa aatcaactgg accaccatcg
    23701 taaacaattc ataacccgtg acaaagatga tcatactgaa aaattcagta aagatgttga
    23761 catggataac gtctttcatt acatacatca accatacaat taaagggaga gctactgcat
    23821 agataccgat aacgccaata tagtaagcgt catagatagc cccgagaaca atcgtggtaa
    23881 tcagtaaata acgtttttca aacgccatac tgcaacataa taatgccaat atcagaaagt
    23941 gggcatttga catataggtg cctttcgacc actcccctag cattcttgtt aaatgaccat
    24001 ctatcaacat caaaaagaac aaaacgattg gcagatagta cttcattgtt tctttcttca
    24061 acatggacta atcctccgct agccgttgga caatcgtcac aactgacacg tcatacattt
    24121 ctgcataagg tttcacataa acttcccgat ccagcccata actatctggt ttcgttttaa
    24181 taaccgtacc gatcggcaag ttagctggag aattccctcc aagaccggat gtctggacaa
    24241 catccccttc tttgatatcc gtatcaccag ttaattgggt caccactaag gcatggagct
    24301 tttcatcata gtttttaagc aaaccaaacg cttcgccatt agccgaagat acccgtactg
    24361 gaaaatgatt ggagctttca ttagaggatg acagtaattc gattttagac gaagccgtat
    24421 tgacctcgat cactcggcca atcaatcctt tttgcgacat gactgccata tttgcttcga
    24481 taccgtcttt agaacccttg tctacgatca gcatgtcctg ccaactgtca ggcgaacgag
    24541 taatgacatt tgccgttact ttttcgtaac tagtcagcgt ctgattcaac tcaagttctt
    24601 ttttcaacgc ttcgatttct ctctcgtaat tcttgttttg ctgtgaaacg tcatcatagc
    24661 tatcgatttt ttcttttagc cgctcatttt cggcataagt tgaaaaaaga ttatggacag
    24721 aatctactcc atttcgtacc caccgtactg gggcagagat tgttttatca accattgcaa
    24781 cactgtcatt aacgatggat tggaagaaat tgggctttcc ttcattcgct cgctgcgcag
    24841 cggtcacact tagtatcgtc accacgatga taacaataac caaggtaata ataatatttt
    24901 tattaggatt gaacttcttc acaaagacac ctcgaattaa ttagactctt gtgttcaatt
    24961 ttatcactaa atgccgtgaa acaaaaggta agttgctcct cttaagcttt tttttagcaa
    25021 ataatagatt ctgtcatttt tagcctgaaa gacaaatttt tttatttgtt tatggatcaa
    25081 tctttgcttt gaataactgc aatcatacca tctgaaaaag aaaattctgc ggtatctgaa
    25141 agaaattcgt tacttgcata agatgtcgga tattctactt ctttattttc caatacatac
    25201 ttgctgcctt ttaatgtaag attcgagacg ggagtcaagc agcaataagc taaatacttc
    25261 atatctggta tctgtcttac tatatgctct ccaggcaaat aataagacaa ataattttga
    25321 caatctctga taataagctg atgggcaaag ggtttgaagc gaggttccat acctaaccag
    25381 agattggcta aaagatgatc caatcttccg cccgtagctc caatcaaagt gacttccgct
    25441 tgcgggtaac gttcaaatgt ttttaagata cctaactgtg tatctgtatc gtctttttct
    25501 gcaggggaac gggtatactc tttggctttt gcaaatactt ccttctgctc ttcgttcgac
    25561 aatgaatcaa aatcacccac tgccagatct actgggtagc ctgcttgaag caggtacagc
    25621 gcaccacggt ctatcccgac ataggcatcg tatttggaca gcagagactg cggccagttt
    25681 tgcggattac ctccagcagc taataaaatt ttcatttagt ctaatgcctc ttttaatcgt
    25741 tcgatttgtc cgactggatt ttcggctcca tagatatatg atcctgcaac gaagacatcc
    25801 gcccctgcat ctttacagat ttttgctgtt tctggaacga ttccgccatc tacttcgatt
    25861 gcataagtcc aattgttctc tctacgtaga tcagcgagtt ccgtaatttt ttctactgtt
    25921 tcttcaataa agctttgtcc gccgaaccca ggattgacag tcatgactag tacttgatca
    25981 gccaaaggca aaacatgttt gatcatggag actggtgttc ctggattgat aactactcca
    26041 gctttaactc ctaagctttt tgtcatttga agtgcccgat gaatatgagg agttgcttcg
    26101 acgtggacag taatgatatc tgctccagct tttgcaaatg ctggaatgta gttttctgga
    26161 tcaacgatca tcagatggac gtctaacggt aatttagtaa caggacggat tgcctgaaca
    26221 atatttggcc ctaatgtgat atttgggaca aatcggccat ccattacatc tacgtggatg
    26281 taatctgcgc ctcctttttc cactaactca atgtctcttt gtaaattggc aaaatctgca
    26341 ctcaaaatgg atggtgctat cttcattctt gtttcctcct attttcgttt accttctttt
    26401 tttgcataca ttggttttct tttttcgatt tcttgtaaga attgcaaata attgtcgtaa
    26461 cgtgtctgtg cgataccacc tgtttctact ctatgtttta cttcgcagcc aggttcttta
    26521 gcatgcatgc actctctaaa tttgcaatgg acagaagctt ctacaaattc aggaaattgt
    26581 ctcggcagtt ctcctgcttc aattgtcaaa aagtcaatcg agctaaatcc tggtgtatct
    26641 gctactaacc cgccatagat aggaatcagt tccacatgac gtgttgtatg ccgtcctctt
    26701 ccaagtgcat cagaaatctc tcctgttgct aaattcagtt ccggtgatat ttgattcagt
    26761 agcgtcgatt ttcctgctcc agactgaccc ataaagaccg atagccgttc tgggaagtaa
    26821 cggatcagtt ctgtcgtatc acccgtatga ttgggtacaa tcaccggata gcctatttta
    26881 gtataggttt gcttgatctc ttcgattttt tctgggtcgt ctgacaagtc ggtctttgtc
    26941 aaaaaaataa tcggttcgat cgactcgtat tccaaggtaa ctagaaagcg gtcaagtaga
    27001 ttataagaaa atgcaggctc tacgatactt gtgacaatga ttccaagatc aacatttgtg
    27061 actggtgggc gaactaattg attttttctc ggcaagattt ccagaagata gccgtctgtt
    27121 tgattttcac tctcaaagat cacctgatct ccgacaagcg gcgtgatttt ccgattccgg
    27181 aaatttcctc ttcctcttgt ttgataggtc tctccgtttg cgtatacata gtaaaaaccg
    27241 ctgattgctt ttctgatttg tcctttgata agtgccacct ccgtttttgt ttattttacc
    27301 atacgaagag gaaagtaggt gttattccat gcaaacttct caaagtatat ggcttgaaaa
    27361 ttgatcaaaa gagatagaaa cacaaaaaag agaatgcaac aaatcaatgt cgcattccct
    27421 tctgatatat tttattcagc acttattttg aactttctga ggtgctagct gatgaacttg
    27481 tggagctgga agattcttct tttgaagagt tgcttgtgct actggagcta cttgagctgc
    27541 tagaactgtc tgaagagtct ttttcagaac tagaagacgc tggatctggt cctttagagt
    27601 aaattacgtt cacaaccatt cctttggtaa ttgtcgttcc tgcccctggt actgtacgaa
    27661 tcactttatc tttatccacc gtgtttgaat attcggaagt ctcatgcccg atatattctg
    27721 caccgacact agctaaataa ctttgtgctt cgctttttgt atagccggag atatcattca
    27781 atttaggctc tgttccttgg cttacgacaa aattgatgta agtttcttct gctacaactt
    27841 cacccgaggc tggatcctga ctgatgatcg aacctgatgg tacagtgtcg ctatagtcat
    27901 aagtagcact aatattattg atattgaatc cttgtgtaac aagccaatta cgaacagttt
    27961 cgtagttttc tcctacatga ctactcaaag tcactgtttc tggtcctttg ctgactgtga
    28021 aagtaatcgt gtctgtttta ggatcgactt ctgtatcttc tgatggagat tgcgaaatga
    28081 tcattccgct ttctacctct gaactgaatt cctcttcttt ttgaatcaag tcagcagaaa
    28141 accctaattc tttcaaactt gcttcggcat cttcataact tttaccactg taatcatcta
    28201 atttgatttt tttcgtgccg ctactaatat acagagtgac ttcgcgtttt tgcttgactg
    28261 tcgttcccgc tgccgggtct gtcttcacta cattaccttc ttcgattttg tcatctgcaa
    28321 tttcttctgt ttttgttgct acttctaatt tcgcttttgc gagtgtttct ctagcagaag
    28381 cttcagaaag atcagaaaca tcaggaacaa caacttctcc tcgcccgcca gaagcaaaga
    28441 atacagctgt tccgattcct aaaacagcaa gaatagctaa taggatcagc caccattttt
    28501 tctgtccttt ctttttagga tcaggcagaa cttcttcctc ctctggtaag gtttcgtttt
    28561 ctggcttgtc ttttggcaga ggcatagatt tgaatgaatc aggcattggt gcatcttccg
    28621 taaggggtgt gataactttt gtttcattat ccatcacatg aggctgccat ttttcttcgt
    28681 ttgcacgagc tggtgataat actgtagaaa gatcatcaga catttcctcg gctgacttat
    28741 aacggtctgc aggttcttta gccgttgccc gtaaaatcac gttttccagt gcttgtggca
    28801 catttggatc aagtgctttt atagatggaa gatcatcttg aaaatgtttt aaagcaattg
    28861 ttacagcaga ttcaccatca aacggtacgc tacccgtcaa catctcataa aggatgatcc
    28921 ctaaagcata aatatctgac tgcttcgttg ccatacttcc tctggcctgc tcaggggaca
    28981 gataatgaac agatcctagc atcgtattcg tctgcgtgat cgatgtttca gataaagcga
    29041 tagcaatccc gaaatccgtg atttttacaa caccttcatt atcgatcagt acgttttggg
    29101 gtttcaaatc tcggtggatg atttgatggt tatgagctaa agaaatagca gaaaggatct
    29161 gctgcataat atttactgct gtttcataag gaacaggata atgtgtttgg atatatcgtt
    29221 tcaagtccat tcctttgacg tattccatca ccagatactg catgttgtct tcttcaccaa
    29281 catcgtaaac actaacaatg tttggatgaa cgagttctgt tgctgctaaa gcttctctct
    29341 gaaatcgtcg aatcgcagtc tggtcatttt gaaagtcaaa tcgtaagact ttaactgcta
    29401 catcacgatc caagattaaa tcgtgcgcca aaaatacatt tgccatacca ccgctaccga
    29461 tatttccagt gatttgataa cggccattta attttttgcc caactcgatc atacactgtc
    29521 ctccttatat tcgataagaa gaacggtgat attatccgct ccgcctgctt cattggcacg
    29581 atcaatcagc tcagttactt tatgagacaa tgggccttct tgattgatga tcgttcctat
    29641 ttcttcttca gacagcatgt tcgtcaagcc atctgaacat aacagcagtc gatcttttag
    29701 ttgccaaaga tgagtagaaa catctacttc gactgttcca ggcatgccga cagaacgtgt
    29761 taaaatattt ttccttggat ggttcactgc catttcttcg ctgatttctc ctgatttgac
    29821 tagttcattg actaaagaat gatcctccgt caattgcatg attttatctt cacgaatcaa
    29881 ataagctctg ctatccccaa cattggcaat cgtgaaccag tcatctgaca gagcggccgc
    29941 tacgattgtt gttcccatac cgttatattc cggctgttct tgtccacgct gataaatcag
    30001 catattttct tcttgaatcg attgaatgaa ccattgggcg attttttcat cgtcaacagc
    30061 ttcttgtttt tcccaagctt cgcctagatt cgttaccgcc atctgactgg caatgtctcc
    30121 ggcacgatga ccacccatcc cgtctgctaa tacagcaagc ttgagacctg aacggctggt
    30181 aaatacactg gcatagtctt gatttgtgtt acgtcgtctt cctacatctg attgatattc
    30241 aatctgcatt ttttcacctc attacttttt gcgcaaacaa caaatgaaaa atccatctgt
    30301 gtaaaactga tgcggataca aagtcaacat tttctcttcg attgatgatt gtagcatagg
    30361 attggtcaaa acatcaatcc tctcaaaatc agggtgtctt tccaagaaag cagaaacaac
    30421 gtcttggttt tcttctttta agatcgtaca agtgctataa gtcagaatac cggaagactt
    30481 taaagttggt gcgcagcttt ctaaaatttc caattggatt tttggtaaat tcgctaattc
    30541 atccggctgt tttttataac gaatatctgg ttttcgccga agcagcccaa gacctgaaca
    30601 aggcgcgtct actaaaatcc gatcaaactg ctctgcttcg aactgtttac tgacttctct
    30661 agcatccatc ttttgagcgt agacagcaga agagacacct agacgtgctg cattatcttc
    30721 aatgagtttg actttgtgtt cgtgtacatc taaagcgacc acttctccac cgcatgacgg
    30781 atctaaaaaa gaagcaatat gcgtcgtctt acctccgggt gctgcacagg catctaagac
    30841 atgatgttca ggctcgattt gcatagacgg cgctacaagc atcgaacttt catcttggat
    30901 cgtcatgacg ccatcgtgga acaatgagct agaagctaaa tgcccttttt tcgctactat
    30961 accgtatgga ctgacttggc ttttttcagc tgtgatctct tcttcttgca aaacagtgat
    31021 ggcctgatct ctggaaagtc ggcgtgtatc tactcgggca ctagcatggc taggctgata
    31081 tagcgataaa ccgagctttt cagtttcttc gtatccgatt tgatccacca gtcgtttcgt
    31141 catccataga ggcaggctga ttttgacaga aagtctttcg ataggatcag caatgccttg
    31201 cagatctggt gctccttgac gctgataatt cctcaagaca ccatttacaa atttccctgt
    31261 cccaggattt ccttttactt ttgcgatttc gaccgcctcg tttaaaatag catgtgcagg
    31321 gactttatct aaaaattcta attggtatac agaaagataa agaagcatct tgacccagtc
    31381 atccactttt tttgctttag cgatcaatgg tgcaacgtaa aattccaata gtcgctgatg
    31441 gctaatcgta ccatagatca attctgtaaa taaacccaca tctttttgat ttagctcgct
    31501 ttgtttgatc atctcattca acaaaagatt cgagtaggcg ccacccttgt tcactcgctc
    31561 taatgtcagt aatgccatat atcgtactga ttttttcatt tttttaggga tttttttcat
    31621 tatttcacct gctgaccaat ctgaacttgc tgaccgcttc cgttaaggaa atcatgtacg
    31681 gcctgttttc ctttaccagc tggctgtaat tcattgatgg ctaagacggt ctgtttaccg
    31741 caagcaatcc atagattttt tttgtctttc cggatgatcg taccaggtgc tttgtctgta
    31801 gtttctgcca atacttcaac cgataataat ttccagcgta tgccctcata ggtcgtgaat
    31861 gcaattggcc aaggacgcat tccgcgtact tggttgtcaa tttcttctgc agttttatgc
    31921 caatcgattg cttcttgttc ccgtgtgatg tttggcgaaa aagttgcttc tgactcgtct
    31981 tgaggtactg gtttttgtcc atccaaaata ttgggcaatg tttctagcaa tagctttctt
    32041 cctaaaaggc taagcttttc aaacatcgtc cctacatcat cttgtttagt gattggtatg
    32101 ctttcttgcg catagattcc gcctgcatcc atttttttga tcatttccat gatcgttaca
    32161 ccagtttctt tctccccatt catgatggca taatgaacag gcgcacctcc ccgatattta
    32221 ggaagtaaag aagcatggac attgattgcc cctaattttg gtacttgcaa taacttttca
    32281 ggcaagaatt ggccaaatgc cgcagtgacg atcacatcag gcaccaattc aatgatttct
    32341 tccatttctg gcgaaccgga gattttctct ggctgcagca cacggatacc gtgcttcaga
    32401 gccgcttttt ttaccggtgt cggcgtaatt actcgtttcc gcccaactgg acggtcgggc
    32461 tgtgttacca ctgcctggat ctcgtaacct gattctatca gcccttcaag aatcggcaca
    32521 gaaaaagcag gtgtgcccat aaatactagc ttagtcatca agatgttcct ccatatattc
    32581 ttcaagttct tcttccggga tctgctgtat cattttctca atgaacaaga taccgtctaa
    32641 gtgatcgatt tcatgctgga aggcacgggc taaatagcca aaagccgtca cttctatctc
    32701 ttctccgtct cgatcataat aacgcacggt cacttcatct gctcgcttga ccgttccata
    32761 gacatgcgga atactgagac aaccttctac gtcaaggctt tcaccttttg cttcgatgat
    32821 ttctgggtta atcagttcaa atttctcgcc ttcatctact tcgatcacag caattctctt
    32881 gttttgtcct acttgaggag cagcgatccc tattccgtca ttcgcaatca ttgtctcata
    32941 taaattgtct aataaagcga ttgtctcgtc cgtgattaca tcgattggct gagcagttct
    33001 ttttaattta tcattagggt ggatcaatat aggatagcgc ataatttggc cctttcttaa
    33061 ataaagttta atggttcgtt gtcaattgcc acaaaaagac cttgtcgctg ttctttctgg
    33121 cttccattca aaatttcatc caaaacatga ggaagattcg gttcatgttt atatttgatg
    33181 attagttgat aataatatcg gtttttcact ctcaaaatca tgctgggcgt tggccctaat
    33241 agcaagcttt gagggcttaa cacttctttt agctgattgg caatctgaaa gattttcttt
    33301 gccgctactt gttcttcagg atggctggcg gttattttca cagtaaagta ataaggagga
    33361 tagcctccac gatgccgtac atgcatttcc tgctgataaa actgctcata attttgtttt
    33421 ttagccaatt ctatggcgta atgttgagga ttgaatgttt ggatcacgac ttcgcctgct
    33481 ttttccgctc gccctgctcg tcctgaaact tgggtaagca attggaatgt atgctcacta
    33541 gaacgaaaat ccggtaaatt cagggaggta tcagcattca acaccccaac taatgtcact
    33601 tctggaaagt ccaaaccttt agcaatcatt tgtgtcccta ataatatatc tgcttctttt
    33661 gcaccgaatt tttgtaaaat ctgttcgtgt gccccttttc ttcgagtggt atctacatcc
    33721 atacgcaaaa tcctagcttg cggataaagt tctcggagtt cctcttctac tttttgagta
    33781 cctgttccat agtagcggat tttattgccg ccacaattag gacagcgatt gggaataggt
    33841 tcttcatgtc cgcaataatg gcagcgcatt gaacgtgtat ccatatgcag tgtaagagag
    33901 atatcgcaat taggacatgg aagaacaaat ccacagtccc tacacatcac aaaagacgaa
    33961 tatcctcgac gattgagcag taggacagtt tgttcttttt tgtctaatcg attttgtatt
    34021 ttttcctgca aattagcaga aaaagtagag gtccgatgat tctgaaactc ttcacgcata
    34081 tctacgatct caatagctgg aagctgtgca gctgctttgg ctcggtggtt taactgcaat
    34141 aactgataaa cgcctttttg agctcgtgct cttgattcta aagagggcgt cgcgcttcct
    34201 agaactattg ggcaatgatg atatttactg cgccaaattg ctagatctct cgcatgataa
    34261 cgcggtgtct catcttgttt gtaacttgat tcatgttctt catcgataat gatcagcccg
    34321 atatttttca gcggggcaaa aatagcagat cgtgcgccta ctactacttg cgcttcttcc
    34381 cgctcaatct tgcgccattc atcatatttt tcaccttggg aaagtccgct atgtaagacc
    34441 gcaactgatt ctccgaagcg tcctttgaat cgctcgacca tttgcggagt caatgcgatt
    34501 tcaggcacta acataatagc cgttttattt tctgacaaaa catcagcgat tgcctgaaga
    34561 tatacttctg ttttcccgct gccagtgatt ccctccagca aaaagacctg atctttttgc
    34621 tgctgacctg ctgtgatgat ttgttcgaca gcattttttt gttctgcatt cagttccaat
    34681 gccgtcgttt ggtcaaatac tcggtcttta taagggtcgc gataacgttc tgattctata
    34741 aactccagcc agcccttttc ttttccttga ttcaagacag cagtactgat tcctttattt
    34801 ttgaaataag caattggttc ttcttggctc agttcagata aacacaaaag aagttctttt
    34861 tgtttcttgg cattttgccg taattttgcc cattcttctt cgatttgctt ttcagttaac
    34921 aaagaacgga ccaaacgaat cgtttttact ttatttcgtg tatggacttc atatttgata
    34981 tctactaatt gctcttggcg ccagcgaatc aattgagaaa ggctgccttc ttcttctgct
    35041 tccttccagg aaatcacttc tcgctgatca aacaatacct gaacttctgg tgtgtctgcc
    35101 aaaggataga tcaatttatc atattctgct ttcatcacac tcggaagcat cgtttgtaga
    35161 caagtgattt tgaatgcgta agtaattttt ttcatataat cagctaatga aagtaattct
    35221 tcattgacca ccggagcaag gtctagcaaa cgaataactg ccttcaaaga tggattagct
    35281 gattcttctg atttttggat agccataaca aatccttgga catgacgatt gccattacca
    35341 aaagggactt ctactcgcat cccgacttgc aaagctgttt ccatttcaga gggaaccaaa
    35401 taagtgaatg gctgatcggt ctgcatagta ggtacgtcca caatgacttc agctgttaat
    35461 gtcatggttc gtttcctcct ttttttgtct tttcttatca agcgacaaaa agttccttgc
    35521 ctattttact agagatcgat ggatttgtct tatcttctgc caaatcctaa caaaaaatta
    35581 acagcagata agaaaaaaaa gaactgtgtc aaaaagttca gacacagcct tttctaccag
    35641 acaataagtg gcgaatgttt gaaaaggaaa cagctgggtt tgcttttctt tttagatgtt
    35701 cgctctattt ttctttatta cattttttgc tcttcacgga tacgtgattc caattcgtgt
    35761 tgttcacgct ctttttgtgc ttgacgttcc tcttcttcca tcttcaaacg ttcacgtttt
    35821 aattcaggat gtggatcatt gactacattt cctgcatcga tttcttctaa agcttgaccg
    35881 acgcttttta ctgaatcaaa agaatctaga gtaggctgtg cacttgcatc caattcatgc
    35941 gcgcgtttac ttgctaaaat cactagagaa tattttgaat ttacacgatc taatagtgag
    36001 tcaattgaag gttttaacat cataagacta catctccttt aacattttaa tatatttacc
    36061 gattacgcga tctactcgaa aatgttcact ggcaataatg tttttgattc gttcaactgc
    36121 tagcggcact tcatcattta caacagcata gtcgtaaaga gccatcattt cgatttcttc
    36181 ttttgcaact ttcattcgtt tttcgattac ttcatctgca tctgttcctc ggcctacgat
    36241 ccgtgatttt aattccgcta aatctggcgg agtcaaaaag atgaatacac catcgggcac
    36301 tttttctttg acttgttttg ctccttggac ttcaatctcc aagaaaacat cttttccttc
    36361 atccaatgtc ttgttgacat atgacaaagg agttccataa taattcccga catattctgc
    36421 gtattccagc atctctccag cttcgatcat tgattcaaac tcttctttgc ttcggaagaa
    36481 ataatcaacg ccttcgacct ccccttcacg cattttacgg gtagtcattg agatcgaata
    36541 ctgaaaatca ttatcctcac tctcaaaaat tgcttttcgt actgtgcctt ttcctacacc
    36601 ggatggacca gacaacacga ttaataatcc acgctctgac atgacgacgt cctttcaact
    36661 agtctttttg tacttatcgt ttattttgca ctttttttct gcaactttca aggttatctt
    36721 taattatagc tagaaattgg taaaaaagct accctgtacc aagaggtcgt aaaaaagctg
    36781 tcctgcatca agtaataaga acagccaaac ataaacagct tctcatgttt ttcgtttggt
    36841 tggggttatt atcgcagatg caagcttttg aacgccgtgt ataagaggtc gtgaagaagc
    36901 cgattagttt cgagtatgaa gaaaaatttt gtgaaaatag tttctcacat ttttgcataa
    36961 atttggctta ataccgagaa aatggttttt gaacatcact tattcaaaat aaaaaaagat
    37021 tgtgacacga attttgtcac aacctttaaa tgtattccta gagcaaagaa gctaagcgaa
    37081 gaaatccaat tctgtttatt tattgtattt ctttgttcag catccttgct aaaagcccat
    37141 attggacaaa gtattcacta cacccatgat ctcatctttt cgagaacgtg ctttttctgt
    37201 cggatcttct acctcttctg tgtcaattcg aacaataaat ttccgattca tcgtcacgta
    37261 agcaccgtcg ttgatcaaaa taaacagttc gttctcatta tagtctgcac aggcatcttt
    37321 ccatacgtca aagccgtttt tttcagaagg gatatcccgt ttaaccacag tttttccact
    37381 taattcgtgg aacgttacgt cataatattt cattatctct acctctttcg tcattcaata
    37441 ttttgtactt gttctctgat tttttctaag atcgtcttag actggacgac ttgattttta
    37501 atttcgattg catttgattt ggaaccgatc gtgttgattt cccgattcat ttcttggatc
    37561 aggaaatcta attcccgacc tacaggacat tgtgcttcga taagttcatc tagtttttgt
    37621 acatggatcg ccagacgatc cagttcctcg tgaatatctc cgcgttccaa caagatggct
    37681 agctccgtca acaaccgttg ctcatcgact ttcgctccaa gaaattcctc cagctttttt
    37741 tgatacctta tttgaaattc cttttcatag atttctacaa aactatcgag tttttttagt
    37801 acttctttga attcttcagt attctttttc aacactaaag ctaaagcttg accttctttt
    37861 tctcgactcg cctcaataga atcaagtgcg cttttcaatg tatctagagc taactgtgcc
    37921 aattcttcca tgctctcttc cggtttttct tctaccgtta cgaagttttc gtccagcacg
    37981 aagcgttccg ccatccgact aattgacact tgattttgac taccgtatcg ttgttgaatt
    38041 ccgtctgtca attgagtgat aagtgaatca atcaattccc agttgatgac gatctgtttt
    38101 tcgtttttgc caatataagc tagattcaca aatacttcga ttcgaccgcg gcttaactgc
    38161 tgtttaataa gctgacgaaa atcgttttca acaaagttca ggattttagg acttcgtatt
    38221 tgaacatcta gaaaacgctg gttgacactt tttatttcaa tatctaactg atacgctttt
    38281 gtttcgcgga tggctttgcc aaaaccagtc atacttttca ttcgacaggg tccttcctca
    38341 ttacatattt tctattgctt tttgcagttg ttcaaactct tcgttcgtca atgtcaggcc
    38401 tttccccatt ttttcatggt ctggcgccca atctcgaaga tcgaattttg gcgggcgacc
    38461 attccagctg actaggttca gttctttacg ccaccccttg ttgttttctg acaatatagc
    38521 gatttcttct acgatttcat atgaaaattc ttgtgccatg gttgttcctc cttgagataa
    38581 tataaatata ggatcagtaa tgtgtcaact ggttgcttaa gcaattcatg ctgcaaacga
    38641 tccttgcgtt ttccttgtag cagataatca aaataaacag ttttcgtgcg ctcattttgt
    38701 attttttgct ggtattttcg ataccttcgc cattcttcag tacttaattg accaatttgt
    38761 gccaataaat catccggcat actttttata tcttgttggc agatcatttg aatcatcatg
    38821 tctacttgat agatttcatt gggcagtaaa aaatgttttt ttgctaaaag ttctagcatc
    38881 gccaatccgc atgtctctgg tacgaccctg ttcttatata aattacgcag tttttgccaa
    38941 aattttatgc ttttgaaaaa agataattct aactgaattt cttttgttaa gtaagacaaa
    39001 aatcctcgcc agtatgttct tgcaggttta acagaatgaa atgtatacca gttcccatag
    39061 taatagccta atcgtttttt catttccaga tagcctaatt gtaaatttaa ttggttcctt
    39121 tggctatcaa aaagcagaaa gctgcctagt gtccacaatg tttgacattt ccaatggata
    39181 aaaatgtctg gcatacgaat ttttttcgct gggcctggcc cttgcacatc tacgacaaat
    39241 gcctctgttg ctcctttgtt aatcgcaata tcaataggta ttttgttgcg atacccaccg
    39301 tctgcatatt tattttttcc gatctttcga taagccatag cgggataaaa tgaagcagag
    39361 gcgagtatcc aatctggtat ttcttctgtt tttgtctgtt ggatatcagt cactacttcc
    39421 gctagttctg gcagtttagt agacaccgta tataaacgta ttttgggatt atgtttgagt
    39481 aaggacaaat ccaaattgtc tttgaccatc ttttccaatg gagcagaaga aaccccgcct
    39541 tcaattagtg ctgttttcgt catttggcgt ctttctcgat agaagcgttc acgaaggtct
    39601 tcaactgcag ccatttctgg taacgcaagc acttgtcttg tcgacagctt tttccataaa
    39661 ttcacagctt ttttcatatc tccttgtaaa atcaatgcgc cgttcaacgc acctaccgag
    39721 gtacctgtga taatctcaaa gttgatatca tgttccttca gcgcctgcca cacaccaatc
    39781 tgataagcac cgtgcgctcc tcctccacct aaaacaagtg ctgtatgata gaccagcttt
    39841 ttatttaatt gtgtggttcc ttgatagccg tttttatgaa aaatacgcaa ccattcttct
    39901 gatagaggag attgaaataa aaaacaacag ttttctttaa taaaaaaacg agcgcaagct
    39961 tccatatacc gaaaaaattt tttccattca aaagtttcac cttcaatcaa cagattatta
    40021 aaaataacat ctcctctact tttagcaata gtaaaataga cggtatattt tttcgatact
    40081 gctttaaaag cacattgctt ttttgcagaa cgccaaaaag cgtctctact ttcttgatga
    40141 ctttgaaaaa atggaagggg ttgttttgct tgctggatag gcgaatacag cgacattttc
    40201 tgtgaaaagt aaactttttt tatctccatc gcgctctcct ttttcttcaa gtataccagt
    40261 acgtcataaa aaggtcatga gtctcgtatt cgactttgta gattccataa gaaaacaatt
    40321 cataaaacct cctttaaata aaaaaataca aaaatcacaa aaattaattg aaaatgagag
    40381 tgttttcaat gaaaacgtga ttttgtaatg gaattttaaa ataaaagaaa caagcttttt
    40441 taatgcaaat gttgtaaaat aaaagagatt aagaggaaat aagaggagga acatgatgac
    40501 tgagtcagtt tatgtacata ttgataccac aagtaatgct gttttaacaa aagggctgac
    40561 tgctactgac tttgcaaaca gtatcgtgca ttttcctcaa aacttgctct tgcttgatcc
    40621 atctgcttct gctggagaat atgaatccca tacagggctg aaagtaatcc gtggtacaga
    40681 aaatattcag cgtttctttt cctcttctcg taacaggcgt ggattcgatg aattgaagtg
    40741 gatcgacttt actgatttaa cgatgctgaa agaattgaca cctttagaaa tttcagaatt
    40801 attatatttt ggtcatatga aaacacatct tcattcgccg tttttttata aactacagaa
    40861 taattttgta tacttcgatc ttaatgatca gctgaatcgg atttattatc gctatttaga
    40921 tgaattttat aggatttttg ctagtaaatt aagttcgatc ttgtcagata agatcaatgt
    40981 aaaaaaatct tttttcaaaa aagaaacacc tgttgaaaaa atcagtgtgg aattattgaa
    41041 agaaatgcgt aatttgatgc aggaagggat cattttttct tttgatcaag ttggtttgat
    41101 cgatggtaaa tat
//
LOCUS       DASNNU010000012        40925 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.012, whole genome shotgun sequence.
ACCESSION   DASNNU010000012 DASNNU010000000
VERSION     DASNNU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.012"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(38..1189)
                     /locus_tag="U8116_000620"
     CDS             complement(38..1189)
                     /locus_tag="U8116_000620"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEN8433636.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="U8116_000621"
     CDS             complement(1202..1639)
                     /locus_tag="U8116_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN8433637.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="U8116_000622"
     CDS             complement(1654..3420)
                     /locus_tag="U8116_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEN8433638.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="U8116_000623"
     CDS             3684..4361
                     /locus_tag="U8116_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8433639.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="U8116_000624"
     CDS             4364..5491
                     /locus_tag="U8116_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433640.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="U8116_000625"
     CDS             complement(5691..6131)
                     /locus_tag="U8116_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN8433641.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="U8116_000626"
     CDS             complement(6142..8175)
                     /locus_tag="U8116_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN8433642.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="U8116_000627"
     CDS             complement(8177..9880)
                     /locus_tag="U8116_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN8433643.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="U8116_000628"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="U8116_000628"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEN8433644.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="U8116_000629"
     CDS             complement(10963..11988)
                     /locus_tag="U8116_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN8433645.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            complement(12197..13066)
                     /locus_tag="U8116_000630"
     CDS             complement(12197..13066)
                     /locus_tag="U8116_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEN8433646.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="U8116_000631"
     CDS             complement(13525..15417)
                     /locus_tag="U8116_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8433647.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="U8116_000632"
     CDS             complement(15414..17141)
                     /locus_tag="U8116_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8433648.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="U8116_000633"
     CDS             complement(17157..17807)
                     /locus_tag="U8116_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN8433649.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="U8116_000634"
     CDS             18011..18307
                     /locus_tag="U8116_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN8433650.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="U8116_000635"
     CDS             18479..19099
                     /locus_tag="U8116_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEN8433651.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="U8116_000636"
     CDS             complement(19191..20357)
                     /locus_tag="U8116_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN8433652.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="U8116_000637"
     CDS             complement(20369..20722)
                     /locus_tag="U8116_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN8433653.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="U8116_000638"
     CDS             20850..21872
                     /locus_tag="U8116_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEN8433654.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="U8116_000639"
     CDS             21891..22052
                     /locus_tag="U8116_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433655.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="U8116_000640"
     CDS             complement(22311..22745)
                     /locus_tag="U8116_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433656.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="U8116_000641"
     CDS             complement(22855..24714)
                     /locus_tag="U8116_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN8433657.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="U8116_000642"
     CDS             complement(24832..25992)
                     /locus_tag="U8116_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEN8433658.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="U8116_000643"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="U8116_000643"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEN8433659.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="U8116_000644"
     CDS             complement(27739..28437)
                     /locus_tag="U8116_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN8433660.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="U8116_000645"
     CDS             complement(28394..30055)
                     /locus_tag="U8116_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEN8433661.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="U8116_000646"
     CDS             30273..31673
                     /locus_tag="U8116_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEN8433662.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="U8116_000647"
     CDS             complement(31925..33004)
                     /locus_tag="U8116_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN8433663.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="U8116_000648"
     CDS             complement(33090..34049)
                     /locus_tag="U8116_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN8433664.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="U8116_000649"
     CDS             complement(34061..35572)
                     /locus_tag="U8116_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN8433665.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="U8116_000650"
     CDS             complement(35594..36157)
                     /locus_tag="U8116_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEN8433666.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="U8116_000651"
     CDS             complement(36163..37026)
                     /locus_tag="U8116_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN8433667.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="U8116_000652"
     CDS             complement(37040..37936)
                     /locus_tag="U8116_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN8433668.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="U8116_000653"
     CDS             complement(38111..39400)
                     /locus_tag="U8116_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN8433669.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="U8116_000654"
     CDS             complement(39372..40889)
                     /locus_tag="U8116_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN8433670.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        11793 a         8580 c         7089 g        13463 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgcc ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgt
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacac
//
LOCUS       DASNNU010000013        40501 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.013, whole genome shotgun sequence.
ACCESSION   DASNNU010000013 DASNNU010000000
VERSION     DASNNU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.013"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1014)
                     /locus_tag="U8116_000655"
     CDS             complement(<1..1014)
                     /locus_tag="U8116_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN8433671.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRS"
     gene            complement(1025..1693)
                     /locus_tag="U8116_000656"
     CDS             complement(1025..1693)
                     /locus_tag="U8116_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN8433672.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1823..2407)
                     /locus_tag="U8116_000657"
     CDS             complement(1823..2407)
                     /locus_tag="U8116_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN8433673.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2738..2971)
                     /locus_tag="U8116_000658"
     CDS             complement(2738..2971)
                     /locus_tag="U8116_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEN8433674.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2986..3936)
                     /locus_tag="U8116_000659"
     CDS             complement(2986..3936)
                     /locus_tag="U8116_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN8433675.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3933..4775)
                     /locus_tag="U8116_000660"
     CDS             complement(3933..4775)
                     /locus_tag="U8116_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN8433676.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4777..5496)
                     /locus_tag="U8116_000661"
     CDS             complement(4777..5496)
                     /locus_tag="U8116_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN8433677.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6188..6334
                     /locus_tag="U8116_000662"
     CDS             6188..6334
                     /locus_tag="U8116_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8433678.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNT"
     gene            6406..7632
                     /locus_tag="U8116_000663"
     CDS             6406..7632
                     /locus_tag="U8116_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN8433679.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(7720..8112)
                     /gene="rpsI"
                     /locus_tag="U8116_000664"
     CDS             complement(7720..8112)
                     /gene="rpsI"
                     /locus_tag="U8116_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN8433680.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8128..8571)
                     /gene="rplM"
                     /locus_tag="U8116_000665"
     CDS             complement(8128..8571)
                     /gene="rplM"
                     /locus_tag="U8116_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEN8433681.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(9090..9557)
                     /locus_tag="U8116_000666"
     CDS             complement(9090..9557)
                     /locus_tag="U8116_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEN8433682.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(9677..11215)
                     /locus_tag="U8116_000667"
     CDS             complement(9677..11215)
                     /locus_tag="U8116_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEN8433683.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11295..11981
                     /locus_tag="U8116_000668"
     CDS             11295..11981
                     /locus_tag="U8116_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEN8433684.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(12182..12709)
                     /gene="msrA"
                     /locus_tag="U8116_000669"
     CDS             complement(12182..12709)
                     /gene="msrA"
                     /locus_tag="U8116_000669"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN8433685.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12793..13470)
                     /gene="pgmB"
                     /locus_tag="U8116_000670"
     CDS             complement(12793..13470)
                     /gene="pgmB"
                     /locus_tag="U8116_000670"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN8433686.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13467..14597)
                     /locus_tag="U8116_000671"
     CDS             complement(13467..14597)
                     /locus_tag="U8116_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN8433687.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(14597..16780)
                     /locus_tag="U8116_000672"
     CDS             complement(14597..16780)
                     /locus_tag="U8116_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN8433688.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(16777..17802)
                     /locus_tag="U8116_000673"
     CDS             complement(16777..17802)
                     /locus_tag="U8116_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN8433689.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(17847..18674)
                     /locus_tag="U8116_000674"
     CDS             complement(17847..18674)
                     /locus_tag="U8116_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN8433690.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(18671..19738)
                     /locus_tag="U8116_000675"
     CDS             complement(18671..19738)
                     /locus_tag="U8116_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEN8433691.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(19764..20600)
                     /locus_tag="U8116_000676"
     CDS             complement(19764..20600)
                     /locus_tag="U8116_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN8433692.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20597..21466)
                     /locus_tag="U8116_000677"
     CDS             complement(20597..21466)
                     /locus_tag="U8116_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN8433693.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(21536..22819)
                     /locus_tag="U8116_000678"
     CDS             complement(21536..22819)
                     /locus_tag="U8116_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN8433694.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(22833..24518)
                     /locus_tag="U8116_000679"
     CDS             complement(22833..24518)
                     /locus_tag="U8116_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEN8433695.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            24793..25818
                     /locus_tag="U8116_000680"
     CDS             24793..25818
                     /locus_tag="U8116_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN8433696.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(26413..30087)
                     /gene="rpoC"
                     /locus_tag="U8116_000681"
     CDS             complement(26413..30087)
                     /gene="rpoC"
                     /locus_tag="U8116_000681"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEN8433697.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(30145..33762)
                     /gene="rpoB"
                     /locus_tag="U8116_000682"
     CDS             complement(30145..33762)
                     /gene="rpoB"
                     /locus_tag="U8116_000682"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN8433698.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            34490..35458
                     /locus_tag="U8116_000683"
     CDS             34490..35458
                     /locus_tag="U8116_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN8433699.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            35476..36381
                     /locus_tag="U8116_000684"
     CDS             35476..36381
                     /locus_tag="U8116_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433700.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            36378..37184
                     /locus_tag="U8116_000685"
     CDS             36378..37184
                     /locus_tag="U8116_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN8433701.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            37320..38309
                     /locus_tag="U8116_000686"
     CDS             37320..38309
                     /locus_tag="U8116_000686"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEN8433702.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(38626..39150)
                     /locus_tag="U8116_000687"
     CDS             complement(38626..39150)
                     /locus_tag="U8116_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEN8433703.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            39437..>40501
                     /gene="scm"
                     /locus_tag="U8116_000688"
     CDS             39437..>40501
                     /gene="scm"
                     /locus_tag="U8116_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN8433704.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        11905 a         8373 c         7214 g        13009 t
ORIGIN      
        1 gctcctcact gattctgtga aaaattggta atccatttct tgcattacta aaaacggcga
       61 tgacaaattt tttttatttt cgattatctt tcctttggta gggcgaataa tcttcatgag
      121 tattcttagt aaggttgttt tccccacacc gtttttccct accaaaccaa taacatctcc
      181 aactttgaca tcaaaattta tgtcttttag aatattttca taagtaattt cttcaacttt
      241 taatacatct gcacttacct tttcgatata gggaacttca atcgaactat taaaaatgtc
      301 aaatttcctt aatgcgttat tgttttcagt ttttaacaac tgaatagttg tagtttttac
      361 agttctattt ttaacaaata gaacttgatc aattaattct ttcaaatagt aaaaacgatg
      421 ttcagctata acaatagtaa atcctttatt ttttaaatca caaagtaagt cagcaagttt
      481 cttagtcgct ttgaagtcca aatttgaaga cggttcatcc aatataatta atttagagtt
      541 tagcgataaa ctacatccaa tagaaatttt ctgccgttca ccacttgata gcgaaaaaat
      601 gtttttatct aatatatttg aaagatctaa taattcagct aattcattga ttcttttatc
      661 catatgctct tttgtaaatc caaagttttc catcgaaaat actaactcac tcgttgtatt
      721 tgttgtaaag aattgtcctc taggatcttg acttacgtag ccaatatttt ttgatatact
      781 ttcttgtgtt aaggattgta gcgagttatt ttcaaataaa atttccccaa ccaattttcc
      841 gtcataaagt cttggaataa gtccattcat taactttaat agtgtggatt tcccactccc
      901 agaagaacca gtaatcaatg taagactgcc tttttttatt gaaaaattaa ggtcaacaag
      961 cgcttgttcc ttaacatcct tatatgaaaa agaaacattg ttaaattcaa acaattcttt
     1021 cacctcaaat caaaatactt aagaaaaaaa tagtaacgga aaacaggatg atatagtcta
     1081 ataagctcat gtttgtttta tagtaagaac ttcttttcac aggagattct atcccctttg
     1141 ttattgcatt agcggtcatt tcttcagcaa tcatagttgc acgtataaga acgggaacta
     1201 acagaaattc caaatattgc agaggctgaa aaaaagacaa gcgaatgttt ctcattttta
     1261 aagattcttt aatcattctt atttcagaac gataagtcgg aaagaatctg atcactactg
     1321 taatacttaa cattattttt ttcggtaaat gtattttttc taaagaggat aataattcat
     1381 tgctagtata gacactaacc aacaaccatg agatcattac gaccggcaac attcgaaata
     1441 caatatataa ggatgtcgca aaaaatatag taaaaaactc tatcttgata gcatcaataa
     1501 ttttcattac taaaaacaga actgaataaa tcaagatgaa ataaatacat gtctttattt
     1561 tacgaaacat caatactaaa actgtagcaa taatgaaaca ccccacttga aatatatttc
     1621 cctttcctaa aaaaataatg accggaaaaa aaatatttaa agtaatcaag gttaacggat
     1681 tgatttttga cactctttca cctccaaaaa ataagatcgc actgttatct taagctgata
     1741 aaaatgcacc gttattcaga taacagtgcg atcttatcaa aaagcataac tgagaacaat
     1801 tctcaatgca agcttttttt aattaactca gtaattcaat ccacccagga acagaaaatt
     1861 gatacaatct tctggttaat agcatagtct tttcttttga atagctatgc ttcaatatat
     1921 catagaatgt agcgtagaat gagtaaatgt agaagtggat ttcttcttca ttaaagacta
     1981 aattttgatt gatcattccg atagtcttta acttatctag acaacagact gtctttttcg
     2041 tcacaaatga tgtaattttt tcaacaatat cttgatattt ggttccagct gattggaata
     2101 aaatcaaatc gaatatacgt ttattctcaa aaatataatt aataaatgga tctaattcat
     2161 tgaagttctc tctccaactt ttctctatat ctatattttc taagtttaag aggtatttgg
     2221 taaactcttc attatacata ttaataagcg cattaatatg cggctgaata atttcttcaa
     2281 ataacgcttc cttagaagta aaatgtttat aaaaagcacc cgtagttacg cctgcggtca
     2341 ggcacagttg ccttatctta gtttgctgaa atccttcttt ttcaaaatgg cttagtccac
     2401 tttctattaa cttttggtga gttataatat aatctctggt ttctatgggt acttcctcct
     2461 aataagatga attaacttta ttatcttaca caggacttga agtttcaatc gttaacgttt
     2521 attactgaac tcattcctta gagctaaact aatgagaaag agcagaataa ctgatacaaa
     2581 aagcaatcct acaagaatta atagactcat ctaaatcatt caccttttct aaaataatcc
     2641 gagaaagatt taaaaaaatt atatccagct attaggataa gtcctatcat aatcactcca
     2701 atagccattt tcttatcctc cctaaaaata aagtctttta cttaaaacct tttttgtaat
     2761 cgtcaataaa ttttttccct ttatcagtta attgaaatcc tatatctaaa tagtcctcat
     2821 atctaagttt agccagcatc tcacttacag atgctggact aacacttaga acttttgcta
     2881 aatcattatt ttttaatttc ttttttcttt ttgataattc ctgaattgct agtaaataaa
     2941 ttagtatagt agtcgtttta atttgtacca tacctaccct ccaaattatt tcgaaaggcc
     3001 ttcagcaatt ttatccaggt tccatttcat catcgcatag taactatcac catcttgtcc
     3061 tttttctgca attgaatcag taaaaatcgt tgaatagata ggaatattgg tatcttttga
     3121 tactgttttc attggtctat catccacact gctttctacg aataaggagg gaacttttgt
     3181 tgtgcgtaat ttttcaacta agtgttttat ttgatctggt gttccttctt cttcagtgtt
     3241 gatttcccaa atgtaagcag aaggcacatt atacgctttc gagaaatatt taaagcatcc
     3301 ttcacttgta acaatcattt ttttatcatt cggaattgaa gcaaatttag atttagcttc
     3361 tttgtctaga gaatccaatt tttcaatata cttatctaga ttttctttat agaatttttt
     3421 attatcagga tctttttccg ctaactgttt ttcaatattt ttagcgtaaa taataccgtt
     3481 ttctaaattt aaccaagcat gaggatcttc cttccctttc tcactctgac cctctaagta
     3541 aataacatct atgccatcac ttgctgcaaa atagtctttg ttttcctctt tgttcgcatt
     3601 tttaactaat tttgtaaacc aagcatttcc tccagtttcc aagttaacac cgttataaaa
     3661 aatcaaatct gcttttgaag ttttttgaac atcttcaggc aaaggttcat attcgtgggg
     3721 atctttccca ataggtacga tactgtgtaa atcaatttta tcttttgcta tattttcggt
     3781 aatatctgct aaaatcgaat tagtaactac tacttttaat ttttcgttcg aagccacttt
     3841 atctttgtct ttactattta ttgaacatgc cccgaatata aaaagagcaa tcaaaatccc
     3901 cagacttccc agtactgctt tccattttct catcataacg atctctcctt ctttcttaaa
     3961 aatttttgtt ttggtgagat tagaaaacta attacaaata agaatgccgc tgttaaaaca
     4021 atacttgatc ctgctgctat gttaaaacta taaccaataa acaatccaag caatgatgaa
     4081 cttgctccga ctgttgcaga aataaatatc atagttttca agctatttgc atataaatat
     4141 gctgtagctg ctggtgtaat taacattgca acaattaata tagttcccac actttgcata
     4201 gctgttactg ctactaaagt taatagaacc atcaatagat aatgataaaa actgacattc
     4261 atccctattg cttttgccat taatggatca aaagaagtga ttaataattc tttaaaaaat
     4321 agaactacta taaccaacac taaaaccgaa acgccaattg taatccactt atcaatatct
     4381 tgtacagcta aaatatttcc aaataaaata tgaaataaat ccgttgagct atttgcaact
     4441 cctataagga taactcctag tgctaaaaaa gaactgaaag taataccaat tgctgtatct
     4501 ccttttatta cactattgct ttttataaaa gtaataatga tagaagctaa tagaccaaat
     4561 acaatcgcac caataaaaaa attaatacct aaaatataag aaattgctac tcctggcaaa
     4621 acagcatgag aaatagcatc acccataaga gacattcctc ttaaaataat aaaacaacct
     4681 actgttccag aaacaatccc tataactaca gatgtaatta gagcattctg taaaaagtga
     4741 aagtcataca gaccattaat aaaattttga agcatattat tccccctctc caataaatat
     4801 agtatctcca taggcttttt ttaaattttc ttcattaaat acagattcta cacttccgtg
     4861 agcaattaga tttcgattca aaagaataat attatcaaaa tacttcttta ccttgcttaa
     4921 atcatgatgc acaattagca ctgtttttcc ctgatgtttc aattttttta acgtatccat
     4981 aattattcgt tcactaacta agtctatccc tacaaatggt tcatctaaaa atatgaaatc
     5041 cgcattttga gcaagacatc ttgctaaaag tacacgttga aattgtcctc cagataattc
     5101 tcctatttga tgattagaat attttcccat atcgaccttt tccaaagcct ttgaaactct
     5161 ttgccattct gcatttttaa ttctttgaaa aactttcatt ccggcatatg ttcccataga
     5221 tacacattct tttactttta ttggaaaagt aaaatcaata tcagcttttt gctctacata
     5281 tgcaatcttc accagttttt tattgatatc ttcattatta atggagacct ctccctgatg
     5341 agggataata tttaacattg ctttaagcaa tgtggatttt ccagcaccat ttggaccaat
     5401 aatacctgtt aaagcacctt cttgtatttt taaagaaatc tcgttcaatg ctaaaaaatc
     5461 gttataacaa acattaagct ttttcacttc taacataact ttctccttct aaatgataat
     5521 gattatcacg atcatatggt atacaaaaat tatattttag tcaaccctaa attttatagt
     5581 ttagattttt ttctgttgct gaagattaga atgacaaaac tatttgaaac tacgcgtata
     5641 gtaagtagct ttcaaaatat ataaaaaagg agcgttctca aatgagaaca tgctcctaaa
     5701 actactttat tggcgtatgg aaaaattata cttgaagtgg atagcgggtt gaataagttg
     5761 ataagcatca aaacatttat aaaaattatt caagactatg tctgttccat atacaaatat
     5821 gaaaatcagt aaactccatt taactttttt cgtagtgtgt ctttttatac atgtttatcg
     5881 ttattaatag tgaataggaa aaaaactatt tttgatcatt gacaattaaa tacaattttt
     5941 gaataacgat ccctgctatt cgagctagaa actcaagtac gccatgacct gattagcgac
     6001 aaatacttag agatttcttt ttgttctggg aaaacaaaat gccatgacaa tagcagcaaa
     6061 taatgatact tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc tatgtgctta
     6121 cccaaagcaa ttcaaaaatt tcataactac tattttagga gatgatttta tagaatccag
     6181 agtctacctg aataaaaaac aaacctgtgc atttctagga atcgataata atactctaga
     6241 taagtggatt cgtatatata atttcccagt aataagagtt ggtggtgtag ttcgctttga
     6301 cgtagaagat attaaagact ttatgaatac ttgaaatttt cttctcgccg catatactaa
     6361 aattagtgaa atatactgcc ttactttttt taaggagtga ggattttgcc tacaaagctt
     6421 tcaaatggga aatataaaac taatctgaga tatccaaaaa ggtttaaaga aataacagga
     6481 attgcatcag aaaagtatca aaaaacattt ccaaataggc aattggcgat taaagccgaa
     6541 aatgatatga agaagaaaat agaaaaagtt ttgagagagg agaatgcaaa ctcattagaa
     6601 ttaaaaggaa aaataacgtt taaaaaattt tacgaatcta aatggcttcc tagatatgaa
     6661 ttaggtcaaa caattagatc aaacaggcct cctagtgata taaccattag taatacaaaa
     6721 gatatatttc ggttgcatat tttacctatg tttggtgaat acgcaatgaa ttatctaaat
     6781 atcaatactg agataatatc agatgaatta accaaaaaat cgaaagaata tgcaaatata
     6841 aaaatcataa aaggatatgt tcgttccatg ttcgatattg cagaaatact aaattatatc
     6901 gaatttaatc gaactactaa aattattcaa agtatcactg cacctaaaaa aaatgcttta
     6961 gaagaaaaaa gaatacaaga aggtaagcaa gcactatcat caaaagaact tactaattgg
     7021 atagaagcag taaatgatga ttttaataat catttactta cttttcatga ttacacactt
     7081 tttatgatta ccctgtatct tggtgataga aaaagtgaga cttacgcttt gcaatggaaa
     7141 tatattgatt ttgaaaaaca aacggtccgt cttaaacata ctttagataa atatcaaaga
     7201 aaaaagttta caaaaggtcg aaaagatacc gtcattcaag taccagaagt tgtaatgaca
     7261 ttgctaagtg aatggaaatc ggtacaagct gatcaattgt tgaaattaaa aattaagcaa
     7321 acgttagatc agtatctctt tacttatact aaaccatctg gagaagtaaa ttgtcctgtt
     7381 catgccgatt acctaaacta tcgaatcaat tcaattaaaa gaaggcatcc tgacttagtt
     7441 catttatctc cacacaaact ccgtcatact tatgcaacta tagctagaca aggtggagca
     7501 gatatgaatc aaatttcaaa tgctttaaca cactcagata tttctacaac aaagatatat
     7561 gtgaacacac cagatatagt tgataaggct gtttttgaag cttttcagag aggattgaat
     7621 aagtgtgatt gatatgtgtt cttaaatgtg ttcttaaaaa caaaagaagc actttccaaa
     7681 taattgaaaa gtgccagaaa cgttgatata taaaggatat taacgttttg agaattgtga
     7741 agctttacgt gctttcttaa gacctggttt tttacgttcg accatacgtg cgtcacgagt
     7801 aagtaagcca gcacgtttaa gagctaaacg gaagtcagga tcaacttcta gcaatgcacg
     7861 agcgatacca tgacggatag ctcctgattg tccagcgtat cctccgccgt ttacgttaac
     7921 aaatacgtca tatgcgcctt ttgtttcagt aacagcgaaa ggttgattga ttacttcacg
     7981 caagtcagca tgtggaatgt attcttcaac atctttttta tttacagtaa ttttgccagt
     8041 tcctggtact agacgtacgc gagcaactgc gtttttacga cggcctgtgc cgatatattg
     8101 aacttgtgcc aatgaaattc cctcctatta gattaagttt gtgatgtcta aaacttctgg
     8161 ttgttgtgct gcatgtggat gttctgctcc accgtatacg ttcaattttg taaattgttt
     8221 gcggcctaaa gtgtttttag gaagcatacc ttttacagaa gtttcgatca aacgacgaga
     8281 atttttagca cgcaattcgc cagcagtgat tgatttcaat cctcctgggt agttgctgtg
     8341 acggtaataa attttgtcag tcgctttttt acctgttaat ttcactttgt ctgcattgat
     8401 gacgatgacg aaatcgccag tatccaaatg aggtgtgaaa gttggtttat ttttaccacg
     8461 tagtacagat gcaacaactg ttgaaagacg tcccaatgga acatcagttg cgtctactac
     8521 ataccattta cgttctactt cgcctggttt ggccatatat gttgtacgca cgttattttc
     8581 ctccaattta agttctgatg tttgacatac acttcgagtt tccggggctc tttgtggggc
     8641 aaacaatacc atttaacata ttacccgcta atctagtcaa tgtcaacgtt tttctgcttg
     8701 attgaaaaat ttatatgaaa ctttacttct tttctttctt ttatataaaa gggtcgtttc
     8761 ctgtcaaaat gaaaaacaaa aagaggctgt gacaaaagtc ttgtcacaac cccttatttt
     8821 gaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc ataaaacata
     8881 agaagctgtt ttttaaaatt gtttcttgtg actcggagct gaacggcagg cttacaacct
     8941 cttggtaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat
     9001 gaggaactat tttgacgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
     9061 tctttttcgg tcaagttcaa caacagtttt tacgcgtcga tctctggcgc tttgcccaaa
     9121 tgagcttgga tcatccagat acgtttttct gtggctgttt tgaattcgat aaagatatct
     9181 tgtgttggat cgtcgccttc ttctccagaa acttcgattc ctttttggta aagatctgct
     9241 agataacgat agcctgcaac aagtgtttcc aaacgttctg gaatagtacg atcccagcgg
     9301 ccaatttcat cagggatttt tgtgttatct gcaaattcac gcaatgtaga ataaggtgaa
     9361 ccgtctaatg tgatcaagcg ttcggatact tcatctaact gagcgttcaa atcatccatg
     9421 aactcatcca tcattgggtg caatgtaagg aaacctggtc ctctcatgta ccaatgtgtt
     9481 tggtgaacaa caacagacat ttggcttaga tctgcaacta attgatttaa tacttctttg
     9541 gtttgttgga atttcatctt tcttgcctcc taacattttc ttacacttaa aataatagta
     9601 gactttcaat gaaaagtaca acaaaatcat ttacctgaga acgattttca gttcgctttt
     9661 ttatccgtaa agccaatcat ttttcagaat cagctaattc ttctcgcagt tgagcaaatt
     9721 gctgataagt ctgtgctaaa tgcttttgtg tcgaaacaaa taatggatag attttctgat
     9781 attttttatg atcagtttct tgtactccaa cttgctcttt caattctatc aattcctgtg
     9841 cttcactgat actttggatc tttccgatgc ttctccagcc taaaatcaca gctcctaatg
     9901 cggatgcttc tgtgacagta gggaaaacaa gcgggtaacc cagtacatca gccatcatct
     9961 gacgaaaaat cggagattga gcaaacccgc ctgtagcacg gatctctgtg agctgatttt
    10021 cctggcaaac tccttttaaa atccgataaa ggttgagaca gatcccttct agaatcgcct
    10081 tcatcatttc tttttctgta tgttgccgct gtaaaccgat aaaactgcca aacgcatctg
    10141 cgtcccaaag cggtgctcgc tcaccaagta aatagggatg aaaaatcaga ccattggcac
    10201 ccgctggcac aaaagcgata gccgaaagca gtgcatcata tggattttct cctttttctt
    10261 tagcagattg aacgacttcc tgtaaaaatg tatgccctgc ccaatcaaag atgccagcac
    10321 cattgctagt cgctcctccc actacccaat gtttctgatc cagaacataa caaaacgtct
    10381 ccatttctgg atggaactga ggctgagtag taataaaacg aagtgctccg ctcgttccta
    10441 cggtcaaagc ggcgatcgta tcaccaaaag cacctaaccc tagattagaa agcggcccgt
    10501 ctgcccctcc caaaacaaat gatatatcat ttggcaaacc tagttctgct gctgtttttg
    10561 atggtagacc tgttacttga taggtggaag gaaagacttg cggcaattgc tctattcgaa
    10621 tgttcaagta gtcaagcact tcttctgccc attggaattc atggatatca aaatatccag
    10681 ttcctgaagc tgttccgtag tcgctgatca gttcgccaaa caatctataa aagatatagt
    10741 ctttgatacc gacaaaataa gcagtttttt tgaataattc aggttggctg ttttgcagcc
    10801 acttgatttt gttcaatggt gtcattggat gaatcggcgt cccagtctgc cgataaaatt
    10861 ctttgccaag cacggtatat ttcagttttt ctgcttcgtc cgctgctcgg ttatctgccc
    10921 aagtgatcat tcgagataac ggtgttttct cttcatccaa caatatcagg ccatgcatcg
    10981 cagaggagaa ggagacaaat tgaatgattg tgtctgaagg caactgttta attacttctt
    11041 ttataacggt gcagacacct tgaaagatca attcctgatc ttgttctgcc atcccttgtg
    11101 tttcttggat caaagggtag cgaaaataag cggatgctgc ttcttgggct tcatctgtga
    11161 aagcaactgc tttggtttgt gtggttccga tatctaatcc gattgaaacg ttcatttttt
    11221 cacctcaaat tttatgtatg tacaaaaatt tagtttaagc ttgcgtatct tggttttgaa
    11281 aagggggaga tggcgtgtta ctttatttta tcattggttg ctgtatcgga tcatttttat
    11341 gtttaaccgc ccagcggatt ccgttaggtc actctattat ttatcctaga tctcattgtg
    11401 tcaaatgctg tcgttcgctt tcttggtatg aattgattcc gattctctcg atcatcgtcc
    11461 aacgatttcg ctgccgctat tgcaggtgtc ggttgccctt ctactatttg ttggcagaag
    11521 ccttatgtgg tggtcttttt gcctggttct ttactttttc ttccgcaaga aatttttcga
    11581 cgttcatatg gatgctttcg gctttgctat tttcgttgat ggatttattt tacttcatgg
    11641 tccattctca cacgctattc tgttcttggg caattttatg gattttttgg ttgcaggccg
    11701 gcgtcttcca atggcaaagt gtgctgttaa cgtttatcgt aggcggtgtg tgccttcgct
    11761 atggtcaatc ctatctcgga gctggcgata ctctgttgat gctttcttgg agtggtggct
    11821 tgtctctaga agagttgctt cagatcttgt ttcttgctag tctgtttgga atatccgttt
    11881 tttctgttta ctttatctgt cataaaaaaa agttggaaaa gctgcctttc attccttttt
    11941 tgagcagtgc gttgctgatt gtccttcatc ttaggtattg aaaaaaggtt gtgacaaacg
    12001 acttgtcaca acctctgata tatagtgttc tcttaccaaa tccttgtgac ttggagctag
    12061 gcgacaggtt cacaacctct ccactaaatg gtgttcaatc agctgaccct cagtattagc
    12121 tcaaaatgct ggttttacaa cctctgatat acggtgttcc ataagtagct ttttcggctt
    12181 cttatttctt gacgccccaa tgtcgacgaa ggaaacctgc acgtccagaa ccttctcgat
    12241 aaagattgta atggagcttg tttttcttgt aataatcctg atggtaatct tctgcaggat
    12301 aaaattcgct tcttggttcg atttttgtca cgattggctt atcgaatcgc ccgctgtctt
    12361 gcaatgcttg ttttgaacgc tctgcaatat ttcgttcttc ttcatcatta tagtagatca
    12421 ctggacgata agaatcaccg cgatcagcga actgtcctaa tgcgtcagtc ggatcggttt
    12481 gctgccaata gatttccacc aactgatcat aagaaataac ttctggatca tattcgatct
    12541 ctactgcttc tgtatgccct gttgtgcctg ttgttacttg ttcataagtt ggatttggta
    12601 catgcccgcc agtataaccg gatgtcacag aaatgatccc tggctgctct tcaaatggtt
    12661 tgaccataca ccaaaagcat ccgcctgcga aaactgcttt ttttgtcata taaaatcccc
    12721 cttttttctc atcatacctt gatttccaaa aggaggataa cgttttgctt gtgacagaaa
    12781 aaacggaaac tattatatac gtgatacggc ttctgataaa tgccaaaacg aacgtacttg
    12841 atcaaaaacg gttaaatcag gaatcacata ttcagcagaa ggaagattta tgttttcacc
    12901 tattcccaat acatgcatgt taccagccaa agctgcttga caaccagctt ctgaatcttc
    12961 aatgaccaag catgcatttg gcggtacatt caattgctgt gctcctttga gaaaaacttc
    13021 tggatcaggt tttgcttttg atacttgcgt gccgtcaata aggacatcaa aataacttgt
    13081 caaattcgtt ttttctaaaa tgaggcgtgc atttttgctt gctgatccca atccgatctt
    13141 gatgtttgtt tttttcaagt actcaagaac agctattgca ccaggcaaaa cggatgtttc
    13201 atccattttt tgaatcgctt gaagatatag attgtttttt tgctcagcaa aggctgattt
    13261 ctctttttct gagaatttct gttccatttt tccaaatgac agcaatcgct ctagtgactc
    13321 ggttctggat attcctttca gttgctcgtt ttctttctca gtgaatggaa tggacagctg
    13381 gttagctaaa accagccacg cttcgtaatg ataacgagct gtgtctacaa gaacgccatc
    13441 taaatcgaat agtgctgctt tgattttcat cgattacctc cttgaaaatg aacgacttga
    13501 agtaaagatg cgatgttttt agctgtgcga tgaagattat cagtggcttg ctgtaaagct
    13561 tcttgtaaag gcacagctgc aggaaggatc ggaaaaacgg ccgaaatgcc aacatctgct
    13621 gctttttcgc ttcctttacc aacacttccg caaatagcta tcacaggaac gccctctggt
    13681 actcttctag caatcccaac tggcgctttt ccttttagac tttgtccatc tagacgtcct
    13741 tcgcctacga ttacgaggtc tgcttgctgt acccgtttat cgaactccag taaatccaat
    13801 acgtagtcga tgccagagac tattttccct gaagcaaatg ctaacagtcc tgcacccatt
    13861 ccaccaccag cactactgcc cgatgcctcg attacttgag gggcaaatct catataaaaa
    13921 tcatgcatgg cttggtcagc cactgctaat tcattttctt tcaagccttt ttgtggtccg
    13981 aaaacaaaag aagcgccttc ttttccgcat aaaagattag taacatcagt aatgacagtg
    14041 attttggctt taatcagctc aataggcacg ttcgttttat caactgtagc aattttacct
    14101 agattttctc ccagtgcttc tactttttga ttatcttgat cgtaaaaagc ataacctaga
    14161 ccttctgcca tgccgatgcc gccatcgttg gtactacttc cgccaacgcc gatgatgatg
    14221 tgtcgaacac cttgttgcaa caattgtcgt atcagttctc ctactcctcg cgtggataac
    14281 ctaagtggct gttgttggtt tagtgggaca tctttcaatc cgacgatttc tgccatttca
    14341 aaaatcgcag tttgaccgtc tgaagcgtac tggatttctt tgtttttgcc aaatgcatta
    14401 gtaacgactg ctgtttgccg cttcaacctt aaactgtctg ctaatgatga aaccgttcct
    14461 tctcctccat cgcctatagg tagcagatca tattcagcct gaacaaaaac ttcttgaaaa
    14521 cctgattgaa tggcttgtgc tgcctctaag gctgtcagac tttctttgaa agaatctggc
    14581 gcaatcagaa tacgcattat tttcccctcc gttccaagat cagttctttt tccagctggt
    14641 attggtctcc attgacaaaa ataggtaatg tttctttcgt ctcttttttt attactattt
    14701 gtttttttgt caaaacaaat agcaacctgt gatttttcca aataaaagga aaagaaattt
    14761 tttcccatgc tgaaggaagt cttggagaaa tagaaagagt tccattcgtt actgtaatac
    14821 ctgaaaaacc aaatataact gctagccata gtgctcctaa tgaagcagca tgaattccgt
    14881 catctgatga atgcggatct ggtcctaaat caatcaggca cgcttcttgg aataaatgat
    14941 acgcccattc catttcttgg atacggcaag cctctatggc gtgaatcgct ttactcaacg
    15001 atgagtcgtg gattgttttg ttttcatagt aatgcaaatt cttagaaacg attggttgag
    15061 aaaaatactc aggtaataag tataacagca tgacaagatc tgcttgcttg ctgacttgga
    15121 gttcatttac ttcgctacgg gcataatcaa gtaaaataga ctggctgcca ggacgttctt
    15181 tgtaagtggt caaatcaata actggcttac tgagatacgt gtcatcttgc ggaatcagct
    15241 gttctttgtt aggcactggc aaatataact tatctaaaaa attttgacat tctttcaaaa
    15301 aaagcggatc gccagcttga tatttaacag ctaaggccac attataatgc gccagatagt
    15361 tcgtatatgc attattgtcg atatattctg tgtattcatc tggaccaatg actctgtgta
    15421 tctctaaatg atcatcgact tttacagctc tgctcagcca aaaagctgct gtttcatgaa
    15481 tgagcgtttt tccctttgtt tctaaaaacg attgatcctc tgttgcttgg acatactctt
    15541 gaacggcata agcaaggtct gcactgatat gatgttctgc ggcagctgaa gcaacttctt
    15601 gtcttgttcc ggttttgata ttcaatgcag caaatttggg tgtctcttcc tgaccagtgt
    15661 gtgcgctttc ccatgggaaa agtgcacctt gatagtgata atctcttgct ttttgccgtg
    15721 cgccttctaa tcgatcataa cggtacagca gcaaattctt tgctacttca ggaaactgat
    15781 ataaaaagaa aggcaggata aaaatttccg tatcccagaa aacatgccct ttataacctt
    15841 cgccagttag tcccttcgcc cctacactaa cacttggatc attgcgggga gtcataatct
    15901 gtaaatggta agcagcgaag tccatcgcta actgatcgaa agaagaatcg ccttggatcg
    15961 ttatccgatg ctgatgccaa aaatctgtcc aataagacat cgactgctct aaaatttctg
    16021 tataactaac gcttttctgg gcttgggcaa tacttttctt aaacgggtct gtttgctgaa
    16081 gagaggtaga gagcacgatg atcttctcca gcgtaaagga cgtatttttt ctgatctttt
    16141 ctgaaaagga agcggtgatc cggcgatttt ttgcttggaa tacacctgct ttattgaatt
    16201 tagctgcaat cccaatatca attttacttt ctgttgtctg gtaggatgca ctgatcaaat
    16261 cgtcaccaaa tactcgcagt tctttttcaa ccaactgctg tgtaccaaaa ttggtttgct
    16321 gtccatcgat acccgttttc acatctactt ggcagtcttc ggtcaacggc ttgatgacca
    16381 ctttcagggc tgcctgatgg agattatttt gatcagcaac acgacaagta atgacttcca
    16441 ctttttttcc ttgagcagtt tcaatgagcg catagcgttt aagctctccc atttctaaat
    16501 ctagtgcccg ctcgtatgca agaactttca tcgtctcaag agaaaaatgt tcaccgtcga
    16561 tttttatttc ccatctgatg acatctggga gattcactaa ctcggctgat tctgctccta
    16621 aaggtcggtt atatacacca gctgcaaaca ttccccttac ttgattccga tagtcttcct
    16681 cctggcttcc tctgatacca aaaaatccat ttccaatggt catctgactc gcatacttat
    16741 tgatatactc taaatcaaac tgtttttctt ctactttcat aaggtaataa cttctcctgt
    16801 ttctgccgaa cgatataaat tttccaccag attctggatg acatatcctt gccagccatc
    16861 tgcaatcatc tgagtctggt caccgagcgc ccgattcaca aaatgagcca tacttagttc
    16921 atgtgggtca ttaactggcg tggtcttttg taaggaaacc agctttccat ctacatcttc
    16981 gaatagttcg cctggataca gattagcacc tgcctgatcg ccacaaaaag ccacattcag
    17041 tttgtcttct ctgatattca aagcaaacga agtattcagt tggatgatct tgccgttttc
    17101 taattccagt gtgccaaaaa ttgcatcttc tactgtgaat ttgcttggat cccatttccc
    17161 gaatttccct gaattttttt tcgtaccaat tttttggaag ctgtacgccc gaacgctttt
    17221 tactttagga aatccagtaa tatacatcgc tgtatcaagc atgtgaatgc cataatcgat
    17281 caaaggcccg cctccttgaa gttctttgtc gataaacgtt ccccagcctg gaacaccaga
    17341 ccgacgaagc gcattcgctt ctacgtaata aatatccccg agaacatcaa gatgattctt
    17401 gatcattgtt gcttcgtcag aaaaacgatg ctggaatcca taagccaaaa tctgcccgtt
    17461 tttatcggct gtctctgcca tctttcttgc ttgatcagct gtcatagctg gtggtttctc
    17521 gcacatcact gaagcattag cttcgagggc agccaataca tgttcgaagt gaaagcgatt
    17581 gggtgtacag acactcacga tatcgggaga aatctcattc agcatttgtg taactgatgt
    17641 atacgcatga gaaaatcctg tacgtcggca aaagtcttct gctcgctggc tgactggatc
    17701 aaccacagcg gccaattcta agacatctga tcttttttga taataagcag catgtacttt
    17761 ttctgctact tgtccagcgc ctatgattgc tactttcttc atttatttct ccctcctttg
    17821 cttatttact tattgttatt ctttctctat tctttctcta aatcatttaa atgacgcgct
    17881 gtttccagac agtttttcaa gtattgcaat gattcctgat aaattttcac ttcattttct
    17941 cctcgcaaac gacattcata taccatataa ccttcatact cattttgaag tagcgtttga
    18001 aaatggcgag taaaatcaat tgcgccagat cctggttggt agcgatgatt atctgctaaa
    18061 tggatatgac ccacatactc tttttgcgta gtcaatgctt ttgaaatatc atcttcttca
    18121 atattcatat gataaaaatc agcgatgatc tgcacatgtt tcaattgatt agagtcaatg
    18181 taatgacgcg catccgctaa caaattgatc atatggtctt ggtatcggtt caaaggctcc
    18241 aaataaataa ccgtacctgt ttcttcggct actttatcta aataacgaag tgattcgctg
    18301 actgctttat agtctccttc tttgcttcgg ggtgaaacca tcggcggtaa tcgataagtg
    18361 aacattcccc aagctgctgg gataacgatt cctttgcctc caacctcgct tagtgcacgc
    18421 aaaatctcag caatttgacg tacaccatta agacgccgtt cttcgataaa gtcaccgatc
    18481 cacccatcgt agccgttaca agctgtcact actgggatcc ctgtttcttt gattgcctgt
    18541 ttgactgtgt cgatattttc tattaacaac ttaccgtcga tttcgtattc ttcaaatcca
    18601 cagtctttga taaatctaaa tttttctaaa atgtctcttg ggaaaaatgc ttgatcttgc
    18661 gtaccgaatt tcattgttct cctcctcgga cttgaacgcc cattttgata ctttcttctg
    18721 ggtgctggtc tacataacgc atgtaagctt ctgcgctttc tgcaaatggc acaactggat
    18781 caatgatttc ttcacaatcc aagtaaccgt tcatcaataa ttcccaacat gtgtcctcga
    18841 ttcgtttacg gttccatctt gggtaatctg ggtttggttc actagatgct cgagcaaaga
    18901 cgatttttgc attattgaaa tgtgcttcgc gaccaagatt aaacccagct ggaaaaggtt
    18961 tagcaaaggc aacataagaa atgatcccgc catagccgat ccctcttaaa gaagcttgta
    19021 atgcggcagc actgccactc gtttcaataa ctgaatctac tccacgttta tctgtcaatt
    19081 cctttagacg aaaaccgaca tctgctttca taggatctag cgtgtaatcg gcaccgtatt
    19141 tttctgctaa ttctctacgc tgcgctatgg gatcgatccc aacgatggta gtagcacctg
    19201 cttttttagc taattgaatc gcaatttgcc cgatagcgcc taaaccgaag attgctacag
    19261 catcacctgc tcgaacatgt gcttcccgga taccacctaa agcgaattga gcaggatcgt
    19321 aacatactgc atttttccat tgatccaacc gatcaagttt acgtagttta taattatcaa
    19381 caccgtttgt aatcagcgtt tcacgaatgg gtccataagt gcagactaac tctccttttt
    19441 gatactctgt caccaaactg cccgtttctt cgatcgtacc gacaatcata tttcctaatt
    19501 gaaattcgcc aaataccaca ccgcgttggc ttccttcttc tcttggcaag aataggcgcc
    19561 attcttctga aaattcttca tcgataaatg gacttaatcc tctaaagtcg acaatttctg
    19621 ttcctggttt tggagtagcg aaaattactc ggatacgtac ttcgttcttt tcgatctcac
    19681 ggtcttcgta atctttgaat ccggctattc ttggttctaa tgcaactaat tttttcatct
    19741 tttctttcct ctttcttttc tttttatcct tttacgccgc cttcgactaa atcaccttta
    19801 ataaatcgtt cagataacgc atacataatg atgactggta aagcggtcac caatgaagcg
    19861 gccatcattc gtccccaaat gtagtctggt gtactgaata atgtgttcaa accgattggt
    19921 aaagtaaatt tttcggaggt cgacaggaaa atcgaagcaa ataaataatc attccacgaa
    19981 accataaagc aataaacgaa gacagaaatg attccagaaa tagccaatgg tataatgatc
    20041 cgaataatga tctggatccg gctaagacca tcgatcattg ctgcttcttc aatatcagtc
    20101 ggaatcgtgt caaaataact ttttagcatg aatatcgcag taggaagtgt ttgaacgacc
    20161 atcgtgataa tcagtgctgt attggtatca tacaaaccta gaccggaaat gattttaaat
    20221 aatggaacaa cgatcaaaat accggaaaac atataaacgg tgtaaaagct agcattgatt
    20281 ttcgagcgag ctttgaagcg tagttttgac aaagcgtatg ccccgctgat cccaatcact
    20341 actgcagtta cggaggctat caaggagatc actaaactat ttttgaaata catgatgaat
    20401 ggaaaaatct cagcattgaa aatatcctga taatgttcaa aagtccattc tttcggaaaa
    20461 atagtttgtg tcgttgcaat tgcttcttgt gaatccttga atgatgtcat cagcatgatc
    20521 aaaaatggaa acaaattgac agctaatagc aacaaaatcg tcgcgtaaaa taagattttc
    20581 ctgctctttt tatttttcat ttcaagtcca ccttctttcg tgagagcaag acgaccgctg
    20641 caataatgat gaacaataga atcgatatag ctgctgcttt tccaagatca ttgaacgcaa
    20701 aggctgtctg atacaaataa acgcctaaga tattgacttt attcgtcagt agatagacat
    20761 ctgtaaacat atagaacatc cagatagatc gtaaaacaat cacagttgct agtactggtt
    20821 tgattgccgg cagtgtcacg atcttgaact tctgccaagc atttgcaccg tccatttcag
    20881 ctgcttcata aagcgttttg tcgactgttt gcaaaatagc caaaaatgaa ataaacgcat
    20941 acggatagta tctccagata gcaaacaaaa cgaccataaa gaagctagca actggctggt
    21001 cgaaccacag cggtgcttcg ttgaaaagct gcaacctatc tactaacaga tcattgacga
    21061 ttccataact attattgaac atatatttcc aagcaaaaat caaagaaata gatggcgtaa
    21121 cataacttaa taaaatcagt gaacgcaccg ttttcctaaa tctgaacttg cggttaagca
    21181 aaatagctac ggctaatcct aaagctgtac tgcctgctac aactaagaat gtgtaagcaa
    21241 ctgttaaacc taaagaacga taaaattcac tatcacttaa aatttcaaga taattgctta
    21301 atccgataaa aacggcgtct gagttaggat tcagcggcat atcgaaaaaa ctgatctgaa
    21361 tattggaaag cattggaatc agcactaaaa cagctaataa aagcatgctt ggtgctaaaa
    21421 gtcccatggc taattttaag tcagaatgtt tccttggacg attcattgaa acctccccct
    21481 gataatctgc caatagctat ctactttttt cgaacaccat tggcagaact tcttgttatt
    21541 tcgtattttt ttgtgcgtca gctaaagctt gatcgacatc ttcgtttccg acagttactt
    21601 ggttcactgc ttttccgatc acacctgagc ttgtgatatc tcccattttc gtgaaattct
    21661 tttccccgac tagtccaaag acttgtactt catcaaaagc actagcaatc tcagatggca
    21721 gctcgccaaa agacttgatg acttcatttt ctttgtaacc atccagttcg actacttgtt
    21781 tattcactgg ttgagcacca ccaggagaca tcaagaccca cttttccatg tttttcggtt
    21841 gagataagaa agctgtgaat gcttcagcag cctctttttg tttctcgtct agaccagaag
    21901 aaatcgtcaa tccagatact gtgccataga cagcttgatc tttttgtgtt gggatagcaa
    21961 aaccgatatt ttctgagttc cccgcttcat aaacagaagg caaaatgtaa gttgaataga
    22021 ttgccattgg tgctgttcca ttcatgaaag catctttgat ttctgtcaca tcattagagc
    22081 ctggcatcgt gtattgggat aagttttgat aaaacgacaa tgcttctctc atcttttccg
    22141 tatcgatcgt tacctcgcct tcatcatcca aaacatttgc tccatttgac aaagcaaatt
    22201 gagagaacgc ttgctcactc attgttcctt cagcagtagg catagcaatt ccatattgtt
    22261 tattctcttt atctgtgaaa tgttgagcaa tttttaaaat atcatcccaa ttttctggtt
    22321 ctgaaaaacc agcttctgat aatttttctt tgtcatacca aataccttga acccagccgc
    22381 taattggtgc agcaatatag ctttttccat cttctgaacg aacaaggttt ttggctccgt
    22441 cataataatt atcttctcca gcttcctcga taacgttttg gactgccgtc tgatcaatca
    22501 gttcatcttt atccatcact ttagcaaaat cctgactgac ttccattact tctggtaatt
    22561 taccggaacg agctaacgta acgatttttg tattgaacgc tgattcttct accggcactt
    22621 gcttgatttt gatgtctgga ttttcttttt caaaatcagc aaccagttca ttgatcacac
    22681 ttaatcgttc ctgttcgaca gaagagtgca tgaattgaat cgttgtctgt tgtgattcgt
    22741 tagagctttg actttcacca caagcagtaa gaagaaatcc agtagctagc agacaggtag
    22801 taacaataat ttttttcatt tttttccacc tcttatttta ttattttcag ccacatatat
    22861 tcatacggct ggaatatcaa ctttcgttct tgactgcaat ggataatttg tccagaaaac
    22921 aggtctaggt aatctccttt tggtaaaaga taacctgctg attgattgga aaaattatgg
    22981 ataactaaga ctttctcttc cttcgcttcg cgaataaaac tgataaaaga atctgctgag
    23041 tccagtaagt aaatcaaact attcggatgg aaggcttttt ctttttttcg aatagatagc
    23101 atctgggtca attcaccata tactgctgtt cttaatggat ctgtctgcaa ctctttctca
    23161 atatctgcta acaaaaattt ttgccgattg atttttcgat tctctcctgt tttttctacg
    23221 cctttgtaat catttcgact tcccagcaca ctttgaatat aaattgccgg cacgcctggt
    23281 aacgttacca aaaatgaatg agcagctaaa aagcgtttgc ttctccattc cgtcgtatct
    23341 gttggagaac ttaacgcatc tagatacgta gcgtttatct catatggact ggtcgttcca
    23401 tctgggtttt tcttgtagtt tactcgggta ccctctttct tcagctgttc cactaattca
    23461 aggatcgttt tttccggcag aatacctcgt accggattca atccgatacc gtcatgggag
    23521 gctaaaaaat taaaaaaagt tgtttttttc gatgcgaaat cgatggtttt taaccattct
    23581 ttcaacttac ttgtattacc cgaacgaatc gcttgaaggg tcaaaggtgg tagcgaaaac
    23641 tgatagacca tctgtgcttc attctcgcca ttcccaaaat aactgatatt atctttgtgt
    23701 ggcacatttg tctcagtgat caataccgtt cctggagctg cttcatcagc gattgcacgg
    23761 aataattgga tgattttatg cgtttctggc aggtgtatac aagaagttcc tagttctttc
    23821 cataaaaatc ccacagcgtc caagcgaata aatgaagcgc ctttttctaa gtaaaacaaa
    23881 agaatatcga tcattttcaa taaaacttct ggatttttaa aattcaagtc tacttgatct
    23941 tcgctaaaag tcgtccatac gtgtttttct ttcccgtttt tgtcaatgaa cgttgtaagt
    24001 aacgggctgg tccttggtct agtcacgctg cttgtatctg tatccagtgg caaatcaata
    24061 aaaaaatctt tgtacgtatc attcaagttt aaatattcct gaaaccaagt actttcggca
    24121 gacatatgat tgcagacaaa gtcgaacatt aaccgtgttt cgcttctcat ttcttctata
    24181 tcttcccatg tgcccaaacg gtcgttgatt gcctgataat ttacaacgga gaaaccatca
    24241 tcagatgtat atgggaaaaa tggcaagaaa tgtacaactc taaatgtatc tgccaaatac
    24301 ttttgataaa atttcttaaa tgtacgcaaa gttggttgct gctcttcaaa aaattgatcc
    24361 ccataagtaa tcaagaaaat atcttcttcg tcccaatgca gtttgcctgc ctggcctttc
    24421 ttctgctgat aaacactgac tttttgttca atcatagagg cggttttctc agacaaatct
    24481 gagtcgcgat aaatcgcttg taaaagtttt gtatacatcg gattcccctt tcttttttgg
    24541 gagcgttccc aacgatttga tcatattatg gaaaacgctt actgtcaata gcattttttt
    24601 aaataatttt gatatacaca aaaatacaaa taagaaagct tatgtatcaa taattaatag
    24661 cttcttaaaa aaatatggtc tctcatgatc atgcaagcta ttcctaaatt ttcaaaatca
    24721 tagacaaaaa agaatgtcta ttgtactatg gaattattct aaaacgctcc caaaaaggag
    24781 gtgcatataa aattgaaacc tactatttat gacattgcga aaaaagctaa tgtctcaaaa
    24841 tcaactgtct ccagagtatt aaataaccag ccaaatatct ctgataaagc aaaagcaagt
    24901 gtacttcaag caatcaaaga attgaattat gaaccgagca aaattgcaag aggactgtca
    24961 ggaaatggtt ttgacgcgat tctagcatta tctcatcgta cttctcatac gacctatggc
    25021 aatccttttt tttcagatgt tattcaatcc atttctgccg tttctgaaca aaataacttc
    25081 gacttgattc tacaaacggc aaaaacacca gaagaagagc tagaaaaatg cgcgaaaaag
    25141 atccaagaaa aaatgatccg aggaattatt attttaagct ctacgatgga tgaaacactt
    25201 ctttttgaat tggatcaata caatattcct attgtggtaa tcggaaaagt cggaaacgac
    25261 tacacgaaca tctactcagt ggacacggat aatttccagg atagttacga tttggtggaa
    25321 acactgatca agaatggaca ccgtcatatc ggctgtctcc atgctccgac agaagtttat
    25381 gtatccaatg atcgtctcaa tggttaccgt caatgcttat ttgatcacca cctagatatc
    25441 cgtaatgaat ggattataga cggcggcttt cgcttagaag atagcatggc agcaatgaat
    25501 gatttaatga agctacgaga aagaccaact gcgatttttt ctaccgatgc attaaaatca
    25561 ttgagtattt acaaagtggc agaacaatct ggaaagcaga tacctgatga tctttcgatc
    25621 gcaggattca gtgatccctt attgtcctcc ctattttcgc cacagttgac tcggatagac
    25681 attccgacaa atcagttagg aatcaaagcc acagaacttt tattccaact aatccaccac
    25741 caataccctg aagaaaaaag aatcattctt tcaaccgaga ttactctcac agattcgatc
    25801 caacaacaag aaatgtagaa cagaattata aaagaacaga aacactgagg ataagtcagt
    25861 tactctgttc ttttttcaat cactcatccc tcgttacccc ctcccggtat tttagcgaat
    25921 ttttcttatt ttccatctgt tttagatagc tgtggataac tataatgttt gtgttaaact
    25981 attttttcca cagggaagaa aaaactattt ttaaccacgt tgtgtataag tttttatagt
    26041 tcgttttttt aaacacattt acccttattt caatccatgt tatttgggga tacttatctc
    26101 taaaacataa atagaataac agacatgtct actggttttg ttttattcga ggaattattc
    26161 aataatagct agcaaaatat taaaaataac cgctctatct tcccatcaat ttattttgca
    26221 ttcaaaaaat gatctatctt tacttttgtt gattgacttc tcttggaaga tgatcgttcc
    26281 gataccttaa ttggatacga accgatgttg ttaagatcct ttcccacttt tcttctagtt
    26341 agacaaatta ttttagacaa accaaaaaag aggttgtgac aataatttgt cacaacctct
    26401 tttttaaaat atctattttg tttgattgtt cattgcgtct tctgcagcca tttgcgcttc
    26461 aatatctgaa atactgtaga cattttcgct tgctacaccg acttcttttg gttccatgtc
    26521 acgataacgt tgcataccag taccagctgg gatgatctta cctataataa cattttcttt
    26581 caaaccaaga agaggatctt tcttaccgcg gatcgcagca tccgtcaaga cacgagttgt
    26641 ttcttggaat gatgcagcag ataagaaact atttgtttct aatgatgctt ttgtgatacc
    26701 tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag actagcgtat tgtagttttg
    26761 atctttaaag tctgcgatgt ctaataatgt acctggtaag atatctgtat cacctggatc
    26821 cattacacga actttacgca gcatttgacg aaccattact tcgatatgct tgtcgccgat
    26881 ttctacccct tgcatacggt aaacacgttg tacttcacga agcaagtagt tttcaacaga
    26941 taatacatcg cgaacttgca acaattgttt tggatcgatt gatccttctg tcaatggtgc
    27001 accacggtgg atcatatcgc cttctgctac tttcatacgt gctgtataag gaacagtata
    27061 cgtacgtgta tctgtttttc ctttgattgt tacttctttg gtacgtgttg caggatcttc
    27121 tgagatatcg atgacatccc ctgttacttc tgtgatcact gcttgccctt tcggattacg
    27181 tgcttcaaag atttcttgga cacgaggaag accttgagtg atatcgtctc cggcaacccc
    27241 acctgtatgg aatgtacgca tcgtcaactg agtaccaggt tcaccgattg attgagcggc
    27301 gatcgtacca actgcttctc cgacttcaac gtcagagcca gttgcaaggt tgcgtccgta
    27361 acaatgtttg cagacaccat gttttgtatt acatgtgaag acagaacgga ttgtcactgt
    27421 ttcgacacct gcatcaacga tttgttttgc gatgtcttcg gaaatcaatt ggtttcctgc
    27481 aatgatcaat tcatttgttt caggatgacg tacttcttta cgggtgtaac gtcccactaa
    27541 acgatcttct aatgattcga tgatttcatt tccctcgcgg atggcttgga tttcaagacc
    27601 tcggtcagta ccgcaatctt cttcacggat gattacatct tgggccacgt caaccagacg
    27661 acgagtcaag taacctgaat cggcagtctt caaggctgta tcggtcatcc ctttacgagc
    27721 cccgtgagtt gagataaaca tttccaagac ggatagtcct tcacggaagt tcgaaatgat
    27781 tggcaattcc atgattcgac cattaggagc ggccatcaaa ccacgcattc cggcaagctg
    27841 tgtaaagttg gaaatgttac cacgagctcc agaatcagac atcatgaaga ttggattttt
    27901 cgcttccaaa ccttccatca gtttttgttg gatgctgtct tttgcagcat tccaaacagc
    27961 aatgacacgt tcataacgtt cgtcatctgt gatcagacca cgacggaatt gttttgtaat
    28021 agtttcaact tgtttatggg cattttcaat gatttcttgt ttttcattca gtacactgat
    28081 gtcagcgatc cctactgtga taccggcatg tgtcgaatgt ttgtacccta agtctttcat
    28141 acggtcaagc atcttagaag tttctgttac tttgaatcgt ttgaagactt ctgcgatgat
    28201 gtttcctaag tttttcttct tgaacggtaa tactaattct tgctctttga tatgtgcagg
    28261 aatatcagta ccagcttcaa caaagtattt gtccggtgtt tggacggtca agttgaagtc
    28321 tgttggttca ttcagataag ggaattcagg aggcatgatc tcgttgaaga tgatcttacc
    28381 aactgttgtg atcatcgtac gttctttttg ccattcagta aatggttttt cacctaatga
    28441 gttagggttc acaccgatac gtgaatgtaa atgaacatat ccgttgcgcc atgcgataac
    28501 ggcttcgttt tgatctctga agaccattcc ttcgccttcg cgtccttctt cttccattgt
    28561 caagtagtag ttacctagaa ccatatcttg ggaaggagta acaactggtt taccatcttt
    28621 agggttcaag atgttttgag cagctaacat caacatacgt gcttctgctt gtgcttcttc
    28681 attcaatgga acgtggacag ccatttggtc tccatcgaaa tcggcattat aagcttcaca
    28741 tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa actggttcga atgcttggat
    28801 accaagtcgg tgaagcgtag gtgcccggtt cagcaatact gggtgttcct tgattacgtc
    28861 ttctaaaaca tcccatactt cgtcttcttg acgttcgatt ttacgtttcg catttttgat
    28921 gtttgaagct agttcgcgtt gaacaagctc acgcatcacg aatggtttga ataattcaat
    28981 ggccatttct tttggaagtc cacattggta catcttcaag aaaggtccaa caacgataac
    29041 ggaacgacct gagtagtcga cacgtttacc tagcaagttt tgacggaaac gaccttgttt
    29101 ccctttcaac atatgagaaa gagatttcaa tgggcggtta cctggtccag taactggacg
    29161 tccgcgacga ccgttatcga tcaatgcatc tactgcttct tgcagcatac gtttttcatt
    29221 ttgaacgatg attccaggtg cattcaagtc taacaaacgt ttcaaacggt tattacggtt
    29281 gattacacga cggtaaagat cattcaagtc acttgttgca aaacgtccac cttctaattg
    29341 aaccattggg cgtaaatctg gtggaataac agggataacg tccataacca tccagcttgg
    29401 ttgatttcct gaagcgcgga atgcttctaa aatatccaaa cggcggattg cacgtgtacg
    29461 tttttgtcct tgtgcagatt tcaattcttc tttcaattcg gctgcttctg catctagatc
    29521 aacattatca agtaattgtt taatcgcttc tgcacccata gcagcttgga attcttgtcc
    29581 atattgttca cgtttttcgc gatattcgcg ttctgtcaac aattgttttt tctctaaatt
    29641 cgtatttcct ggttcgatga ctacatatga agcaaaataa atgatttctt ccaaagcgcg
    29701 tgggctcatg tctaatacaa gacccatacg tgaaggaatc cctttaaagt accagatatg
    29761 tgaaacagga gctgctaatt caatatgtcc catgcgttca cggcgaactt tcgaacgagt
    29821 aacttccact ccgcatcgat cacaaacgat tcctttataa cggatacgtt tgtattttcc
    29881 gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc tcacagaaca acccttcgcg
    29941 ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt tttacttcac cgtaagacca
    30001 gcttctgatt ttttcgggag aagccaaacc tatttgcata ctttcgaatt tatttacatc
    30061 gatcaaaagg ggttccctcc atttcattta atggcaaacc gcaagacggc tgatcttcga
    30121 ccgccttgca gctcatagac cggattagtc taaattatct tctgcggttt caaagttttg
    30181 gatgacatcg tctttttcat cttccacttg ttcagcagct ttcttttcta attctttggc
    30241 agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg atcaaatcgt cgtcttcatc
    30301 gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc aagacacgca tgtccagacc
    30361 tagtgattgc aattctttca ccaatacgcg gaatgattcc ggtacacctg gttttggaat
    30421 tggttcaccc ttgacgattg cttcgtatgt tttcacacgt ccgactacgt catctgattt
    30481 gtatgtcaag atttcttgta aagtataagc cgcaccgtaa gcttccagtg cccatacttc
    30541 catttcacca aaacgttgtc caccaaattg agctttacct cccaatggtt gttgtgtaac
    30601 caatgagtaa ggtccgattg aacgtgcatg caatttgtca tcgaccatgt gagcaagttt
    30661 gatcatgtac atcacaccga cagacacacg tccatcaaat ggttcacctg tacgtccgtc
    30721 gtacagaaca gttttcgcat cgctggccat accggcttca cgaactgttt cccatacgtc
    30781 ttcatcactt gcaccatcaa atactggcgt tgctacgtgg atacctaact gacgtgcagc
    30841 catacccaag tgtaattcca atacttgccc gatgttcata cgtgaaggca cccctaaagg
    30901 attcagcatg atatcgattg gtgttccatc aggtaggaat ggcatatctt cttccggcat
    30961 aatacgggat acaacccctt tatttccgtg acgaccggcc attttatccc cttcatggat
    31021 tttacgtttt tgaacgatat aaacacgaac taacatgttt acgcctggtg acaattcatc
    31081 tccagcttca cgagtaaaga ttttcacatc gtgaacgata ccgccgccgc catgtggtac
    31141 acgtaatgat gtatcgcgga cttcacgagc tttttcgcca aagatcgcat gcaatagacg
    31201 ttcttctgca gataattctg ttaccccttt tggcgtgact ttccctacaa gcaaatcgcc
    31261 atcttggact tctgcaccaa tacggatgat acccatttcg tctaaatctt ttaatgcgtc
    31321 ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt cctaattttg tatcgcgggc
    31381 ttctgattca tattcttcaa tatggataga agtgtatacg tcatctttta ccaaacgacg
    31441 gctcatgatg atcgcatctt catagttgta cccttcccaa gtcatgaaac caactaaaac
    31501 gttttggcct agagccattt cgccttgttc catagatggt ccgtctgcca gagtatcccc
    31561 tttttcgact ttctcaccca aatgaacgat tggacgttgg ttgtagcttg ttccggagtt
    31621 tgaacgacgg aattttgtta catcgtattt atctaatgca ccgttgtcgc ggcgtacgcg
    31681 gatttcagaa gcatcaacgt attcaacgac accgtcatgt ttacataaca aagcagctcc
    31741 tgagtcatgc gctgatttgt attccatacc agtaccaacc catggtgctt gaggattgat
    31801 caacggcact gcttgacgct gcatgttggc acccatcaag gcacggttgg agtcatcgtt
    31861 ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact tgtttaggtg atacgtccat
    31921 gtagtctact ttatcaatag atacttctaa gttttcactt tgagcacgag ccataacaac
    31981 gtcttgagca aatgttccat cttcattcaa tgggctgttt gcttgggcta cgatgtaatg
    32041 gtcttcgata tcagctgtca agtagtcgat ttggtcagta acacgacctg tttcacggtc
    32101 tacacgacga taaggtgttt cgataaatcc gaatttattt actttcgcat aactggacaa
    32161 actattgatc aacccgatgt ttgggccttc aggagtttcg atcggacaca tacggccata
    32221 gtgggagtag tgaacgtcac gaacttcata accggcgcgg tcacgagtca atccgccagg
    32281 tcctaaggct gaaagacgac gtttgtgtgt caattcgcca agagggtttg tttgatccat
    32341 gaactgtgac aactgagaag aaccaaagaa ttctttgata cttgccacaa ctggacggat
    32401 attgatcaat tgttgaggtg tcaatgtttc agtatcttgg atcgacattc tttcacgaac
    32461 cacacgttcc atacgtgcta gcccgatacg gaattggttt tgtaaaagtt caccgactga
    32521 acggatacga cgatttccta agtggtcgat atcatcgaca ttgccgatac cttccatcaa
    32581 gttcaagaaa tagctcattg aagcaatgat gtcggctgga cgaaccgttt ttacagctgc
    32641 ttctggatag ccgtttccga tcacgtttac ttcacgttca gggtctcttg gtgaaaatac
    32701 tttgatgact tggacagtca ttggatctgt gaccacaccg tcttctgatg gatagtaagt
    32761 cactgaattt aggccattat caatgaaagg agctagtgtt tccatgactt ggtgagtcag
    32821 aactgttcct ttttcaacga tgatctcgcc tgtttctgga tcaactaatg tttcagctaa
    32881 tgtcagattc aatagacgtg ttttcaaatc taattttttg tttactttgt aacgaccaac
    32941 gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca ttcagcaagt tacgtgagct
    33001 gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa acgtctttca aaccttcttc
    33061 tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg actgtattgc gtaatgtttc
    33121 gctgtcgccg aagatttcaa agatcgtatc atcggaacca aatcctaacg cacgaactaa
    33181 tacggttaaa ggaatcttac gtgtacggtc gatacgtaca taagagatgt cttttgcatc
    33241 agtttccatt tctagccaag ctccacggtt agggataaca gtagaaccga atccttcttt
    33301 tccgtttttg tcgacttttc catggaaata gacacctggt gaacggacta actgagaaac
    33361 gattactcgc tctgccccgt tgatgatgaa tgttccttgt tctgtcatta atgggaaatc
    33421 accgaagaat acttcttgtg ctttgatttc gccagtctcg cggtttgtca aacgcaatgt
    33481 tacatgtaat ggtgcagagt agtttgcatc atgcgcacga gcttctgcta ctgtatactt
    33541 tggttctttc aattcataat ctacaaattc caaagataga ttgccattga agtcatcgat
    33601 tggcaaaatg tcttcaaaca tttctctaag tccctcatct aagaaccatt ggtaagagtc
    33661 tgtttgaatt tcgatcaaat ttggtaattc caacacttca ctgattcttg cgaaacttct
    33721 acgttcgcga tgttttccgt attttactac gtgtccagcc aagctcttca cccctctaaa
    33781 atgtcattca aaaagactac ccgatcaatt gttacaataa tatttcaaat attattttta
    33841 ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa aaccaaaaat agaacattct
    33901 cttgaaacct tacttcgtga gcttctactt ttgtttcctt atattaaagc cgaaacggac
    33961 aatatttcgt ttctgccttt tcttcatcag atagtttttg caacttcata tactacactt
    34021 attttctata aaagtcaaca cttcctggcg tttcttctgc tttttcgcaa cattttctta
    34081 atgttttcaa ttttatgttt tcaattatct gacaattctt aattgacaga cctttttatc
    34141 tcggttatac tttagaaaat tcattttgtg gaggaacttc tatgcgaacc aacatcaacc
    34201 aaatcgcacc aacacaactg aataactttt actttagcta ctttagatag gtttgtattc
    34261 atcatgatgg atacaaacgt taaacgacag tttgtatcta tgatggctaa agaactttgt
    34321 attgcagcaa aaagccacgt agacaaaatg ctaagtggct gagcagtgaa aatacaaagt
    34381 aaaagtatca agcattctac tttttcaatt tattttccga cttttcaatc catttggaca
    34441 ttttgtctga atggattttt tgtttcccaa aattaaggag gaaaaagaaa tgaaaaaaaa
    34501 tataatcatc ggattaggcg ctgccctgct actagcttta ggaggttgcg cacaaaaaga
    34561 caattccgca gaggcatcag atggacaaaa aacaattggc gtattgcaac ttgtacaaca
    34621 taactcgtta gatgctgcct ataaaggatt caaagaagga ttagcagaag aagggtataa
    34681 agaaggcgat aatctaacaa tcgattatca aaatgcccaa aacaatcaag acaacctgaa
    34741 aagtatgagt gagaaactcg tcaaagaaga tcctgatttg ctacttggaa tcgccactcc
    34801 agcagcacaa tctttattaa atgaaacaac cactatcccg atgactgtca cagcggtcac
    34861 agacttggaa caagcccatt tagtcacatc caatgaaaaa cctggcggca atgtgactgg
    34921 cacgacagat atggtgccaa tcgaaaaaca aatcggctta ctgttaaata tcgtgccaga
    34981 cgccaaaaca atcggcatca tgtataacaa cggtgaagca aactctaaga tccaagctga
    35041 tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc aaagtactga cagcaaattc
    35101 cacaaatgat gtccaacaag tgacgaaaag tctagctaaa gatgtagatg gaatctatat
    35161 tcctactgat aacacgttcg caagtgcggc aagtgttgtc ggtgaagtcg caaaagaaac
    35221 aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa gacggcggtc tagcaacagt
    35281 aggaatcgac tacgaaagct tgggtaaaca aacagggatc atggcagcaa aaatcttaga
    35341 cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat gatttggaac tgtatgtcaa
    35401 taaagacatg gccaaagcat taggcatcaa tccagacagc atcaaagaag ctgaataaaa
    35461 cacaggagga ttctcatgac aaatttgctc tctcttttct tatcatctgc atcgcaaggg
    35521 gtcttatggg ccctacttgc gattggtgta tacgtcactt tccgtatctt ggacattgcc
    35581 gacttgactg ctgaaggaag ctttccttta ggtgcaggga tcgcggcagt aagtattaca
    35641 aatggctatc atccattgac agcttgtctt ctaggctttt tagggggtgc ggcagcaggg
    35701 cttgtttccg gactgcttca cacaaaattg aaaattcccg cccttcttgc agggatcatc
    35761 accatgactg gtttatattc tgtcacatcc cgtgtgatgg gtgcgccgaa catctcttta
    35821 ttgggtcagc caacgatctt tcattgggtc gagtcattcg gcataacaaa agacaacgct
    35881 gtactgatcg tcggtctgct ttttgctttg atcgttgtat gtttacttgt tttgttctta
    35941 cgtacagaaa caggacttgc tattcgtgca actggcgata atctggcaat gagcgaagca
    36001 aacgggatca acacagataa tatgaagatc atcggttata tgatttctaa tggatgcatt
    36061 gctctttctg gctccttgct tgcgcaaaac aacggctttg ctgatttaaa ttctggtatt
    36121 ggaacgatcg taattggact tgcttctatt atcatcgcag aagttttgtt acgtaaccag
    36181 ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg tcatttatcg ttttatttta
    36241 gcgttagtct tcgaactgaa tgttgaacca agtgattcga aacttgcttc tgctttcgtg
    36301 ctagtgatct gtctttcttt cccgcagatc cagcaaaaat tccatttacc tcaaaaaaag
    36361 aaaggcggta gtgtcaaatg aatgacgtct taacattgga aaatctccat catacctttg
    36421 aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat taatctttcc ttagatcccg
    36481 gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa atctactttg ctgaacagca
    36541 tcgccggtac attttctgtc gatcagggtc aaattttatt gaatggaaaa aatatcacga
    36601 aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt attccaagat cctaaactag
    36661 ggacagccgt tcgtttaacg gtagaagaaa atcttgctct tgctatgaaa cgaggaaaaa
    36721 aacgcggctt cttccgtggt gtcaaaccac aagaccgaac atttttcaaa gaccagctgg
    36781 ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga aatcggtttg ctgtctggag
    36841 gacaacgtca agctattacg ttattgatgg ccacattaca gcgtccagaa ctgatcttat
    36901 tagatgaaca cactgcagcg cttgaccctg ctacttccaa aacggtgatg gaattgacag
    36961 aaaagctgat cactgagcag aatctcactg cttttatggt cactcatgac atggaagacg
    37021 cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg aaggatcgtc gtagatatcc
    37081 agcaagagga aaaacagcag ctcacagtgc cccaattatt agcgttgttc aaacaaaaca
    37141 gcggcacggc attaaaagac gatcaagtac ttttatcttc ataatcagaa agaggatggg
    37201 acaaaaaatg aacgcccacc ctcttctttt ttctctttcc ttctttcgtt tttgctttat
    37261 gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc cttcttcctg atagaatgga
    37321 tgggaaagga gcttgatgcc atgacaacga aagacaaggt acttcaatta ttgaaaaaga
    37381 caccagattt tctttccgga gaaaagttgg cacagcaact ggaagtttcc cggacaagta
    37441 tttggaaagc catcaaagaa ttggaaaagg caggctatcg ttttgagcac gggcctacag
    37501 ggtatcgtta cctgccctct gacgtactag atgcaaaaga aatccagcaa gagctacggt
    37561 cgtttattcc tgatttgaaa gtacaaacga tgttgacttc tgaatctacg atgaaagatg
    37621 tcaaactggc agcagctcaa ggagattctg tttcgaagtt atttgttgct gatacacaag
    37681 aacaagctcg tggacgtttt ggacggcctt ttttcgctga acctggacga ggaatctaca
    37741 tgagtctctt gctccatcca gacaaacggt ttgatgaatt gcctcaatat acgatcatcg
    37801 cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg tcgttctgct tcgatcaaat
    37861 gggtcaatga tatctacatg gatggaaaaa aagtctgtgg gattttatct gaagcagtca
    37921 gtgatatgga aagcggcagg atcagtcata tcatcattgg gatcgggctt aatttttctg
    37981 ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc catctctgtc tttcccgacg
    38041 gacaagctag tgtcacaagg aatcagctaa tcagcaaaat ctggcaagaa ttgttcactt
    38101 atctttctca cccagaaaaa gcgttggact tctataaaga aaaatcattc gtattgggaa
    38161 aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg tcttgcaaaa gaaatcggtg
    38221 atcatggcga actgatcgtc caaacaaatg aacagaagat gagtttgtct tctggcgaaa
    38281 tcagtctcac atctatccgt acgcaataat tatcagattt cacaaaagga cacctgatct
    38341 catacagtgt ccttttttct gaattctctt caaaagactc tccttattct atcagctgac
    38401 agtttctcta tcctctagaa aaaggtattc taaaaatatc tataaagcat ggtacacttt
    38461 ctttaaatga atcaatgcaa aaaacaagag agtcatcagt ttacacgatc aaaagagata
    38521 gcaggccgct tcctttttca atcttataga tagaccatgg gtaacgataa aaacaaaaaa
    38581 gaacttgcga cttattttgc cgtaagttct tttttagcta gtgccttaat ctcttaatcc
    38641 caacaaacgt ctcgcataga tcacctgacc agagtgcata gcagcatcat caataataga
    38701 aacgagacgc attcctcgtg tgactggtgg tgtccagttc tcgtcaatta cttcatctag
    38761 ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa tcagcagctc ctcgcaagta
    38821 tcctagcaga tcttctattt gattagcttg gatctgcttt gcttgttcca gactatgctt
    38881 ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg ctttcccagt cttgactatg
    38941 ccaaattgga gaagttcctg ctaaatcagc aatctggaaa tctaattctc gagcggtatg
    39001 ccaagccagc caagtcattg atttgatctg cggggctcta tcttccactg gaaaacgatt
    39061 ggcctcctca atagagacgc cttcaaacaa acgttcaaac cgatcgtatg cccgatccac
    39121 actctcgcat agcatatcta aataatccat ttattttcct cctttttatt tatcctactt
    39181 actttttcct aattctagga tacctactta tatgttacta cacaccctta ttttttacac
    39241 tttaacagtc accatcatca atagtttgga gattgaaaga aaaagatagt gtcgaactat
    39301 caattgaaca agaagcaaca atatgaacgt ttcattaata ttctttaaat aaacaatgat
    39361 ttttcaaacg aacagtttaa gactaggaga aaaagagtat aataggaaaa tgtttgaaag
    39421 gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta gtcctagttg ctttgttgtt
    39481 caatatcttt tcgtatggtg tggccgtttc agcggaaaca tacattccta aagaccctgg
    39541 gaatatttat aatgatctaa ctggtaacta tggcttgtta ggaattgcta gtcaattcca
    39601 cgtatttgct aaaaataaga caactgtgaa tgcacataca gacggaaata tagcaacaca
    39661 tgaacttgat gcaaaaaata attttggtac agagcttcat gaaggtttat tgaaccaaga
    39721 aataaattat atacaaaaaa ctgattcttt cgctaattct tcaggcatac ctacaggtag
    39781 cacaagaatg aataaatttg tcgttggcag taacgtggat gtagcacttc aagatggtca
    39841 tcctacagtc aatggatcca ggcttgatca cttaactcca gatgattttt ataaagatcg
    39901 tgctgggcat gaatatattg atttcgagca ggaatttgct aaattgaatg tagcagcaaa
    39961 tgatcttgca acgatcactc cagctaaaac atatactgct gcagattttc cagacatgaa
    40021 caatcggaca atcgatttaa ctgatttatc agataccggg tttttattgg taaatatcga
    40081 tgcagaagtg ttaacgatga atacgccact tcaaatcatt aatccaaatg atcaagtagt
    40141 tgtatttaat gtcatcaaca gttcttctgc attgaacgta cagtcaccaa tcaaatacaa
    40201 tgatcgttca aatcatgaaa cagaagattt ttcggatgcg aatatctctt ggaattttgg
    40261 aaatgagatg actgatttga caatcagtgc tccttttcaa ggaacaatcc ttgcgcctaa
    40321 tgctactatc cgcattactc aaaaccaaga cggaacgatt ataggaacaa atgtgatttt
    40381 agatgcagct accaatcgtt gggatcctaa cgaaatattt atcagcgaca atggtacaga
    40441 tacgacagac acaagcgaca gtacagatac atctaccaca agtgatagct cagattcatc
    40501 a
//
LOCUS       DASNNU010000014        39729 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.014, whole genome shotgun sequence.
ACCESSION   DASNNU010000014 DASNNU010000000
VERSION     DASNNU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.014"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            272..1183
                     /locus_tag="U8116_000689"
     CDS             272..1183
                     /locus_tag="U8116_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN8433705.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1561..2478)
                     /locus_tag="U8116_000690"
     CDS             complement(1561..2478)
                     /locus_tag="U8116_000690"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEN8433706.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2680..3117
                     /gene="zapA"
                     /locus_tag="U8116_000691"
     CDS             2680..3117
                     /gene="zapA"
                     /locus_tag="U8116_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEN8433707.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            3175..3723
                     /locus_tag="U8116_000692"
     CDS             3175..3723
                     /locus_tag="U8116_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEN8433708.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            3838..6198
                     /locus_tag="U8116_000693"
     CDS             3838..6198
                     /locus_tag="U8116_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEN8433709.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            6285..6599
                     /gene="trxA"
                     /locus_tag="U8116_000694"
     CDS             6285..6599
                     /gene="trxA"
                     /locus_tag="U8116_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEN8433710.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6781..8559
                     /gene="uvrC"
                     /locus_tag="U8116_000695"
     CDS             6781..8559
                     /gene="uvrC"
                     /locus_tag="U8116_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEN8433711.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8704..9603
                     /locus_tag="U8116_000696"
     CDS             8704..9603
                     /locus_tag="U8116_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8433712.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9596..10858
                     /locus_tag="U8116_000697"
     CDS             9596..10858
                     /locus_tag="U8116_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433713.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            11019..12917
                     /locus_tag="U8116_000698"
     CDS             11019..12917
                     /locus_tag="U8116_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEN8433714.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(13247..13966)
                     /locus_tag="U8116_000699"
     CDS             complement(13247..13966)
                     /locus_tag="U8116_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEN8433715.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            14303..14635
                     /locus_tag="U8116_000700"
     CDS             14303..14635
                     /locus_tag="U8116_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433716.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14622..16625
                     /locus_tag="U8116_000701"
     CDS             14622..16625
                     /locus_tag="U8116_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEN8433717.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16644..17114
                     /locus_tag="U8116_000702"
     CDS             16644..17114
                     /locus_tag="U8116_000702"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEN8433718.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            17135..17719
                     /locus_tag="U8116_000703"
     CDS             17135..17719
                     /locus_tag="U8116_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEN8433719.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17734..18720
                     /locus_tag="U8116_000704"
     CDS             17734..18720
                     /locus_tag="U8116_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEN8433720.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            18710..19021
                     /locus_tag="U8116_000705"
     CDS             18710..19021
                     /locus_tag="U8116_000705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEN8433721.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            19043..20824
                     /locus_tag="U8116_000706"
     CDS             19043..20824
                     /locus_tag="U8116_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEN8433722.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20817..22193
                     /locus_tag="U8116_000707"
     CDS             20817..22193
                     /locus_tag="U8116_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEN8433723.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            22200..22835
                     /locus_tag="U8116_000708"
     CDS             22200..22835
                     /locus_tag="U8116_000708"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEN8433724.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22865..24220
                     /locus_tag="U8116_000709"
     CDS             22865..24220
                     /locus_tag="U8116_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEN8433725.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(24412..25806)
                     /locus_tag="U8116_000710"
     CDS             complement(24412..25806)
                     /locus_tag="U8116_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN8433726.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            25898..26869
                     /locus_tag="U8116_000711"
     CDS             25898..26869
                     /locus_tag="U8116_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEN8433727.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            26866..27648
                     /locus_tag="U8116_000712"
     CDS             26866..27648
                     /locus_tag="U8116_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEN8433728.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(27817..28188)
                     /locus_tag="U8116_000713"
     CDS             complement(27817..28188)
                     /locus_tag="U8116_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN8433729.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            28322..28765
                     /locus_tag="U8116_000714"
     CDS             28322..28765
                     /locus_tag="U8116_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEN8433730.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(28808..29617)
                     /gene="yidA"
                     /locus_tag="U8116_000715"
     CDS             complement(28808..29617)
                     /gene="yidA"
                     /locus_tag="U8116_000715"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEN8433731.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(29636..31003)
                     /locus_tag="U8116_000716"
     CDS             complement(29636..31003)
                     /locus_tag="U8116_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN8433732.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(30987..31817)
                     /locus_tag="U8116_000717"
     CDS             complement(30987..31817)
                     /locus_tag="U8116_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEN8433733.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            31980..32417
                     /locus_tag="U8116_000718"
     CDS             31980..32417
                     /locus_tag="U8116_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEN8433734.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            32493..33113
                     /locus_tag="U8116_000719"
     CDS             32493..33113
                     /locus_tag="U8116_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEN8433735.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            33302..33808
                     /locus_tag="U8116_000720"
     CDS             33302..33808
                     /locus_tag="U8116_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEN8433736.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            33825..34724
                     /locus_tag="U8116_000721"
     CDS             33825..34724
                     /locus_tag="U8116_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEN8433737.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            34811..35695
                     /locus_tag="U8116_000722"
     CDS             34811..35695
                     /locus_tag="U8116_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN8433738.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(35887..35958)
                     /locus_tag="U8116_000723"
     tRNA            complement(35887..35958)
                     /locus_tag="U8116_000723"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35923..35925),aa:Arg,seq:ccg)
     gene            36018..37352
                     /gene="rpoN"
                     /locus_tag="U8116_000724"
     CDS             36018..37352
                     /gene="rpoN"
                     /locus_tag="U8116_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEN8433739.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            37501..38592
                     /locus_tag="U8116_000725"
     CDS             37501..38592
                     /locus_tag="U8116_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEN8433740.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            38633..39634
                     /gene="gap"
                     /locus_tag="U8116_000726"
     CDS             38633..39634
                     /gene="gap"
                     /locus_tag="U8116_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN8433741.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
BASE COUNT        13098 a         6864 c         8475 g        11292 t
ORIGIN      
        1 tatttttatt tattatgtcg gtgttccagg aagtgtttta atttaataac gaagaaatta
       61 attgtttgta gaatgaagga aactatttat tgggtagatg aatcaccacg aatagtcaaa
      121 aggagatttc aggatatgcc aaaaatgaaa aaaatcattt tagcagtaat gttattttct
      181 acggttatat cttttacagc cccttttttg cttacttaag ctgattttaa gttttggccc
      241 ttagtctgga tctacgaggt gagagtcaag aatgaaaaaa ataattggag cttttgtatt
      301 tgcaatcatt atcattatta tctgttttca aatagatgtt tttggtcaaa aaaagatagt
      361 tggacaaacg gtcagcagta cgaacagtac ggttacaaag acaaaggaat catttgatga
      421 tacagaacaa gagacattaa tcaaaatcgg aaaaaatgac cgtacactat atacagatga
      481 ctcgctaact accccacttg caactataaa cggcggagag ctggcggagt ttctttctga
      541 aacaaaaaat gcttatcaga tcaagacaaa tgatggctat actggatatt tagccctcga
      601 agatggaacc aaagtcacaa agaatataca aacaaaaccg gaagagctat ctgatgcagt
      661 gattgtatta gatcctgggc atggcggaaa tgatactggg gcattaagca atgatgaaca
      721 aacagaagaa aaagatataa cattaagtac agccataaaa gtaaaaaagg cgctagagga
      781 tgctggagca acggtttatt tgacccatac tacagatgaa ttagtacagc ttggagatat
      841 atgcgactac agtgaagaaa aaaaggcaga tgtctttatc agtttacacg cagactctac
      901 ggaatatgcg aatgaagcaa ctggaatcac gacatattat tactatggac aagaggaaac
      961 attagcacag acaattgctg actccttcac tgatctgcct ctggattcaa gaggaatctc
     1021 cactgggaac tatcaagttc ttcgagaaaa tcttcaacct tctattttaa tcgagatggg
     1081 ttatatgaat aatgatagtg atttaaagga attagtaaca gacagttatc agcagcagat
     1141 tgctgcaagt ttgacacaag cattgaccac ttattttcag taataaataa gcccagccaa
     1201 acaaaaatag ctcctcatat ttttcgtttg gccgggctta ttatcgcaga tgcaagcttt
     1261 taaacaccgt ttaaggaaga ggtcgtgagc ctgccgttca actctgagtc acaaggagca
     1321 atttcaaaaa acagcttctc atgtttgatg actattgtga cttgtgaccg aggagttggc
     1381 atgagaacac cgtttaagaa aaaggttgtg aaatcaacgt tttgttctga gtattagaga
     1441 aacaaacgga aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgaggatcag
     1501 atgattgaac accgtttatt cggaacaagg gactgtgaca tgacttttgt cacagttcca
     1561 ttattttttt agtagcttca aagctttttc tgtattggca aaatgaagtt tggcatattt
     1621 ttcaagcata ggcatgccgc ctttctccaa tagttttgcc gctacttggt cagatttcgt
     1681 tccagctcct gacggaaggg tgattccagc ttcagctgat gctttgtcta actcttccaa
     1741 aaagctggca cgactgatga tagaagcagc agcaacagct aaatgatatt gttctccttt
     1801 agtaacgaag tacagttttt cttgtaattg atttttttct aaacgcgcat attttttaaa
     1861 atttgcttcc ggcgtaaact gatcgatcaa aatcgcttct ggtttttctg gctcgatttt
     1921 ttttagcagc aaatggatcg cttgattgtg caaagcgact ttcatccgaa cggcattgta
     1981 ttttggttgg atctcattgt atttttctgg agataagaca agcagtttat aggggatggt
     2041 tttttttaag atgtcagcaa gctggatgat ttggctatct tttaactctt ttgaatctct
     2101 aacaccaagt ttacgtaatt ctgcaagctg atcacgtttc acataagcag cacatactgt
     2161 tagcggaccg aaatagctgc catttccgac ctcatcacta cctaaaacgg ataattgact
     2221 gatatttttt ggcaaagaag aggctgtttt tgctggcttg gcttgtttat cctgtgcctc
     2281 cccccattta tcagcttcta ttgctgcatt cgtaccttga aacatcactt ttccggatgt
     2341 ataagcggta atggtcgtcc cgtctttttt tgcagcaaag ctagtataag gaatctgttt
     2401 atttaacaaa taagagcgat agtatgcttt catttgacta agttggtcgt ttgttgcttt
     2461 gataatgacg ttgctcataa aattctcctc gtttttatac ataaaactat accgcaactc
     2521 acgacttttc gttttctaag agattgcgag acatagcatt tttttaaagt ttatcatata
     2581 atagttaagt atgtgaaaaa tatttgtgga gaacggtgaa agaaccaact tcatggtaca
     2641 ctaaataaaa ctttcatcaa ataggaggaa atgattcgta tggcgcacga aaaaacacgc
     2701 tacaaagcgg tgattgccaa ccaaacatat actattatcg gacgggaaac caagcatcat
     2761 atggatatcg taacgaagct gatcaatgaa cagttagcag aattgaaaca attatcacca
     2821 caaatggata atgaacaagc agcaatcttg atggctgtca atgcgctttc ggatcaattg
     2881 aagaaacaag aaagaatatt ggagctggaa gaagaaacag cagaattaaa gaaaaaaatg
     2941 atcaaattta cagagcttga aaatcgtgtg aaacgcattg aagcaatcga gaacgaagct
     3001 cgggaagtat tgaaagaaaa tggtcaagca gatcatacca ttaaaaatca tgtggaagca
     3061 caacagattt taaacgagaa acgaaaagga caaattaaac aaaaatcttc taattaagat
     3121 ccttttatca aaataagaaa aatttaaaaa agttagaaga gaaaggaacg tagcatgctg
     3181 agtttgctga ttttattttt attgctaatt gcgttttttt ctggagcacg tcgcgggttt
     3241 gctttgcaaa tcatattcgc gattggatat gtattgtcat ttattgctgc ccagcatttt
     3301 tacaaaccat tagcaagtca tttggaatta tacattccat acccagctgt caccccaaca
     3361 tcaaagttag cattttttga tcaggtattt gcctttcacc tagatgaagc tttttatgca
     3421 ggaactgctt ttctgattat tttgctaatt ggctggctgc ttacccgatt tgtgggggtt
     3481 tttgtccatg gattgacgta tgtgccgatc ttgagacagg tagactggat tgcaggaggc
     3541 atcctgtcac taatcatggc ttacgttact atatttttga ttttgcagtt acttgccttt
     3601 gttccattag atgtggtcca aaatcaattc ggcgagcata gtttcgctcg ttttatcgtt
     3661 gagagaacgc catttcttac caacaagatg tatgagcttt gggtaacgaa tattttgaat
     3721 taaggaaaat agtgctggaa aatatttcgt tttttgtaca gagtagttta tttaagaagg
     3781 ctgcgtagcg tatgtccggc acagctcttt tttcatagtg aaagataagg tgataaaatg
     3841 aataaccgaa tccttgaaac attggaattt gaaaaagtca aacagatggt cagacaattt
     3901 gtagtgactg cacaaggaaa agaagaactg gcagaattgg taccagtgag tgagaaacaa
     3961 acgatcacaa actggcttca agaaactgag gatggattga aagtgcaacg tctgcgaggc
     4021 gggatcccta tacctaaaat tgaaaatatc cgtccccata tgaaacggat tgagatcgga
     4081 gcagacttga atggagtgga gcttgcacaa gtttccagag tattgtctac gacaagcgaa
     4141 ttgaatcgtt tcattgatga tctttctgac agtgaaatcg aatttgcacg tttgtatatg
     4201 tgggcagatc agttagtcac gatccctgtt ttgagccgcc gtttaaagga agcaatcgat
     4261 gaagacggac gcgtgactga tgacgcttct cctgagttaa agagcataag acagaacatt
     4321 cggcgaagtg aacaagctgt tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca
     4381 aagtatttaa gtgatgcgat catcaccatg cgaaatgacc gctacgttat cccagtcaaa
     4441 caagaatacc gcggtgtatt tggcggtgta gtccacgatc aaagtgcttc aggtcagact
     4501 ttatttattg aaccaaaaca agtggtggat ttgaataatc gtttgcgtca ataccaaatc
     4561 gctgaacgaa atgagattca acgtatttta agtgaacttt ctgctgaact tgttccccat
     4621 cgtcaagaaa tcatccacaa tacttatgtg attggtaaaa tggatctgat gaatgcaaaa
     4681 gcacgatttg gtaaagaagt aaaagccatc gtgccgggaa tcagtgaaga caatcatgtc
     4741 gtcttgaaac aagcaagaca tccactgatc gatcaggaaa aagtagtttc taatgacatc
     4801 acaatcggaa aagactatca agccatcgtc attacaggac ctaacacagg tgggaaaacg
     4861 atcacgttga agactttagg tctgttacaa ctgatggggc aagcaggatt accgattcct
     4921 gcaggtgaag aaagtcaaat cggtattttt gaggaagtat ttgcggatat aggagacgaa
     4981 cagtcgattg agcaaagtct atcgaccttt tcttctcata tgacgaacac tgtagatatt
     5041 ttatcaaaag tcaatgaaaa aagtctggta ttatttgacg agctcggtgc aggaacagat
     5101 ccgcaagaag gtgctgctct agcaatcgca atcttagatg atcttggtaa aaagtctgct
     5161 tatgtgatgg caacaacgca ttatcctgaa ttgaaagttt atggatataa tcgggcaaat
     5221 acgattaatg ccagcatgga gtttgatgtg gatacattaa gtccaacgta tcgtttgctg
     5281 attggcgttc ctggacgaag caatgcgttt gaaatctcta gccgattagg tcttgataca
     5341 gaagttattg atgaagcgaa gcagttgatg aatgatgaga gccaagattt aaacgaaatg
     5401 atcacagatt tagagaatcg ccggaaaatg gcagagaccg aatatctgga aatgcgtcat
     5461 tttgtttctg aggcacaaga acttcacgat gatttgaaag aagcttacag ctatttcttt
     5521 gaagaacgtg aaaaagaaat ggaaaaagca aagaaaaaag caaatgaagt cgtgtcagag
     5581 gcggaagaaa aagcagaaaa aatcattgct gatattcgta aaatgcaaca gcagattggg
     5641 caaggaaatg tgaaagagca tcagttgatc gatgcaaaga cccaattagc aaatctacat
     5701 caagaagaaa ctttgaagaa aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg
     5761 aaaccgggag acgaagtgtt ggttacgact tacggccaaa gagggacgct tttacgtaaa
     5821 aacgggaatc agtggcaagt agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag
     5881 ctgacgcctg ttgctcctca aaaagagccg acacaacgag ttatccatgc tgtacgatct
     5941 gaatctagta gtcacgtgcc gaatcaattg gaccttcgag gaaaacgata cgaagaagcg
     6001 ctaagtgagg tagatcaata tttggattct gccattctag ctggttatcc tcaagtgact
     6061 attgtccacg gaaaaggaac aggcgcactt cgcaagggaa ttactgatta tcttaaaaac
     6121 catcggagcg tcaaaagctt tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca
     6181 attgttaaat ttaagtaaag cttactaaag gtaagcagat ggttcgctat ccaagtatag
     6241 aaatgctata ctagtcatag attttaaaaa tggaggtctt tgatatgtca caagtaatta
     6301 cagataagac atttaatgaa gaaacagata aaggacttgt attgattgat ttttgggcaa
     6361 cttggtgtgg tccatgtcgt atgcaagcac cgattttgga tcagttggaa caagaatatg
     6421 atgaagaaga attcagaatt gctaaaatgg atgtggatga aaatccagaa actcctcaac
     6481 aattcggtat catgagcatt ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa
     6541 aagctgttgg tgttcattca aaagaacaac tacgtcaaat gatcgatcaa tatttataaa
     6601 aacttacagc aaaaataagc tgagggtggg actgaatcca gttccgtcct cggttttttt
     6661 gcggttgagt taaggttatt caaattaata ttttttgagt ataattacgt tgtcaggctg
     6721 gattgttttt ttgacagaca tgctataata actttaattg aatatgaagg ggagtaccag
     6781 atgaacgaac gaatcaaaaa caaattagct ttacttcctg atcagcccgg ctgttacttg
     6841 atgaaagata aaaatggaac gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt
     6901 gtccgctcat attttactgg tagccataat acgaaaacgg aacggcttgt tagtgaaatc
     6961 gaagactttg aatacatcgt tacagaatcg aatatcgaag cattgctctt agagatcaat
     7021 ctgataaaga aaaatgatcc taaatacaac attatgttaa aggatgataa aacctatcct
     7081 ttccttaaga taacgaatga aaaatatccc cgtttggtga ttactcgtaa ggtactaaaa
     7141 gacaaagcac tgtattttgg accatatccc gatgtcggtg cggcgaatga gacaaagaag
     7201 atcctcgatc gattatttcc tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt
     7261 ctttactatc atttgggaca atgcctgtgt ccttattatt tcgacgtaga tccgaaagtc
     7321 tatacgaaaa tcgttgatga agtgaaaaac tttttaaacg gaggacacga aaaaatcgag
     7381 tcagagatcc atcagaaaat ggaaaaagct gctgaaaaca tggagtttga aaaagctgca
     7441 gaataccgag accagatccg tgccattgag acaatcatga cttgccaaaa aatgacaaat
     7501 actgatttac tggatcgaga tgtatttggt tatgcgattg ataaaggatg gatgtgtgtc
     7561 caagtgtttt tcgtaagaca aggaaagcta attgaacgag atgtttcaat gtttcctttc
     7621 tataacgaag cagaagaaga tttcttgact tacctcggtc agttttatca ggaaaatgag
     7681 catttcattc ctaaagaagt cttgattcct gatacgatcg acaagcccag tgtagaagct
     7741 ttgttagcta cgaaagtgct gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg
     7801 gcgagtaaga atgcctcagt tgctttgaat gaacggtttg atctgattgc cagaaaacag
     7861 gaacggacga tcggagcagt tgaacgttta ggaaacgcga tgaatatccc gaccccaata
     7921 cgtatcgagt cttttgataa ctctaatatc atggggacga atcctgtggc tgcgatggtc
     7981 gtattcattg atggaaaacc ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc
     8041 aacggaccgg atgattatgc atcaatgaga gaagtcatct atcgcagata ttcgcgagtg
     8101 attcgcgaag ggttgccttt acctgatttg atcttgatcg atggaggaaa aggacaagta
     8161 gatgtggcaa aagatgtctt agaaaatcaa ttaggaatcg atattcctat tgctggcatg
     8221 gtaaaaaacg ataaacataa aacaaatgag ttgctattcg gtacagattt ggctgtagta
     8281 ccgcttgagc gaaattcacc agagtttttc ttactccagc gaatccaaga tgaagtccat
     8341 cgattcgcaa tcactttcca tcggcagcta cgcagtaaaa acagttttgc ttcccgatta
     8401 gatgaaattg aagggttagg accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg
     8461 ttgaaaaata tcacagcagc cacgacagaa gaattacaag aaatcggact gccgaaaaat
     8521 gtcgcacaaa acgtatttga taaattacat caggaatgag gaaagggcac tttacaaaga
     8581 aagtaaagtg tcctatcatt ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa
     8641 taataaatcg cttacgtctg gtgtctaagg atatatgtga tttcttgaag aaggagtgaa
     8701 gaaatgttag aagtcaagga tcttgttaaa acttttggga actacacagc tgtcgatcat
     8761 gtctctttcc aaatccccga tggaaaaatc atggggctaa ttggccaaaa cggggctggg
     8821 aagacaacga cttttcgttt gatcttggat tttttgacac aagaccaagg agaagtgtta
     8881 tggaatggcc atcatcttgg ggagaaagat tatgatgtca tcggttatct tccggaggaa
     8941 cggggacttt atccgaaagt aacgattcaa gaccaactgc tttattttgc agaacttaga
     9001 gggaaatcaa gaaaagagat tgaaccaaaa atcgactttt ggatggaaaa atttcaggta
     9061 aaagggaaaa agacggataa agtaaaatct ttgtctaaag gaaatcagca aaaagtccag
     9121 ctgatcgcca cgttaatcca tgaacctaaa ttgatcattt tggatgagcc attcagcggg
     9181 ttagatccgg taaatgctga attgctaaaa gatggaatat tggaattgaa aaaacatggt
     9241 tcatgtgtca ttttttctag ccacaatatg gataatgtag aaaaaatctg cgatcatttg
     9301 atcatgttgc gaaacggaaa aatggttttg aatggaaaag tacatgagat tcgtgaatca
     9361 tttggccgta cgaagttatt cttggaatct ggtttaagcc aacaggaagt agaagaaatc
     9421 gaaggcgtga aaaaagtgat ccttcatgaa gacaatacgt tagagattac cttagaagat
     9481 cctgaagctg ggaaagaaat ctttactcgt gcgactcagt tcggctacat tcctatgttt
     9541 aaccagcaac cgccaacttt ggaagagatt ttcaaattga aagcaggtga aatcgatgcg
     9601 taagttttgg attattacca aagatgtcta tttaaaaaat gttaagtcga tttctttctt
     9661 gatcatgata ttagtccctt ttatcatgct gggtatcatt tatgtagcag gaaactttgc
     9721 tcaacaaaat agtgatgtca ataaaatcgg cgtattggct gataatgagc aaatcagtca
     9781 gcaaatgagc caagtcaaaa cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa
     9841 agcacaaaaa gagctttcgg aagaaaaaat cgatgcgtat ttagtagtct caactgagaa
     9901 tgataagata aatgggatgc tttatagcga aaattcatta ggacaaagta cacaactact
     9961 gatacaacaa caactgactg gcttgcaatc gatgcttcga gcgagtcaat taggcatttc
    10021 accagaagaa gtagctagct tgagccaaca agcaagtttg actcgacaaa aagtcaattt
    10081 tgattcaaaa ggaaaaatga cagtcggaga agataactcg acagtccaat acgtcgtaag
    10141 ttatgtagca acgattgtct tgttcattat tattttgacg tatgcgcaga tcattgcaca
    10201 agagatcgct tctgaaaaag gcacgcggat catggaagtg atcctgtcca gtacgactgc
    10261 acaaaagcat ttttacggaa aattaacagg tgttttactt gttgcattaa cgcaaatggc
    10321 attatacggt attatttttg gtataggata cagtcaattc aaagatatgg aaatggtcaa
    10381 aaatatatta gacggtatct cattggataa cttgattgga ccattcttgt ggtattctct
    10441 tctattcatg atctttggca ttttgatttt ttctgtttta gcagctttat gtggctcttt
    10501 ggtcaacaaa gcagaagata cagcaaaagc aattttgcca gtgacttatc tatctctagg
    10561 cggatacatg cttggattga tactaggagc ttcagatcca aacaatatcg ttattcggat
    10621 cacttcgtat attccattct tatcttcata tattatgccg attcgcttag caaatgagac
    10681 agtcggtatg ggtggagcat tgatttcctt gttggtactt gtagccgtaa cattcttatt
    10741 gatgatcttg tctgcaaaca tgtacaaatc gaatgtactc gtttatagcg aaggcggatt
    10801 atggagttca ttgaaacaat cgatttcaat tatgaggaat gaacgaaaaa aagcataaaa
    10861 caatcctaaa atacagaagg aagctggaca tctattgttc ccagcttctt tttgtataga
    10921 cctattttat ttattttact atattggata tatattcatt gaaaatagtg tataacaact
    10981 ttataatatt gagtatacca atttttggag gcgttttaat ggaccgttat ttagatttac
    11041 tttcagaaaa attcccctca acggaggatg tcatcacgga aatcatcaat ctcgaagcaa
    11101 ttttgcagct tccaaaagga acagagttat ttttgagtga tatacatggc gaatttcctg
    11161 cttttgatca tattctgcgt atcggttcag gaaatcttaa agaaaagatc aaagaccttt
    11221 tttctgaaca aatgacggaa acggatatca gccaattcac tatttttacg gcttatccag
    11281 aatatgcatt aacgactgat tggtacaaag aacaagataa atcgcagctg atccatcatt
    11341 tgatcgatct tttacggttt acgacagtca aatatacccg gtcaaaagtt cgaaaaggat
    11401 tgccgaaaga atatagctat atcattgagg aactcttgta tatcgatgac cgtatcaatg
    11461 gaaaaaaaga ctatgtaaaa aaaatcattg aacaacttgt tttgatgaag gaagaagaaa
    11521 aatttttaac gaaacttgca cagacgatcc aaaaatctgt catcgatcat ttgcatatcg
    11581 ttggagatat ttttgaccga ggaactcaag cagccaaagt gatggatcga atcatggatt
    11641 tttcttctgt agatatccaa tggggaaatc atgatatttt gtggattggt gcgtattgcg
    11701 gctctgaggc atgtttactg acacttttac ggattgcagc acgctataat tatttatttg
    11761 aattagagaa agaatatggg ttgaatttac gtccattgtt ttcttttgct catcaaactt
    11821 atcagaagaa tccagtattt gcgcctaaaa acagtaaaaa tctatccgaa agagagcaag
    11881 aagaattaga aaaaatccat caggcacttt ctatccttca attcaaatca gaacatcagt
    11941 tattggatcg tcgacctgaa tttaaaatgg acgatcgtaa attgctggaa aaaatcgact
    12001 atgaagcatc aacgattgat ttagaaggtc agctctatga gttgaaagga acttgttttc
    12061 aaacgatcca gccagatgat ccaaaaaaat tcttggaaga ggaacaaaaa gtgattgata
    12121 gtttgatgta ttcattccag cactctttga gaatgcaaaa acatatgacg tttttgatcg
    12181 aaaaaggggg aatgtatctc acgaacaatc agcatgtgct gtttcatggc tgtatacctg
    12241 tagacgagaa gggacagctc ttagcatttg aattagacca atcttatcgc ggaaaagaac
    12301 tgctgggctt ttttgaaaaa cagataactg aaagcgcaaa agatctgact gcaaaagaag
    12361 atttagcaac cgatttgatt tggtatgcgt ggagtgggcc attttcgcct ttattcggca
    12421 agagtaaaat ggcgactttt gaacgttatt ttctcacaga gcctcatacg cacgtcgaga
    12481 aaagcaatcc ttattatcac ttgcgagatg aagagtggtt cagtgaaaaa attttagcag
    12541 aattcgacac aaaagaagaa gggtcagcaa tcattaatgg gcacacgcca gtaaaagtaa
    12601 aaaaagggga gtcaccgatc aaagcaaatg gaaaagtatt tgtcattgac ggaggtttat
    12661 ccaaagctta tcaaaaaacg acaggaattg ccggttattc actattatgc aattcttatg
    12721 gtttccaaat cgtgacacat tatccgtttt tgcctattga acaacaattt gcaaaaaaat
    12781 ccgaccaaac aaatgtgaaa aaagtaatcg aacagcaact accacgcaaa ctcaatcgtg
    12841 atacaacaaa aggtatggaa ctgcagcagc agattaccca gctcaaaagg ctgttggact
    12901 atcgaaagga cgattgagaa gaattccagt gaacattgtt ccatagactg gagactgaga
    12961 caaaagtcgt gtcgcagtct ctttttccgg ataaacggtg ttcttctgcc aactcctcgg
    13021 tcacaagtca cactattcaa caaacatgaa aagctgtttt ttgaaattgc tccttgtgac
    13081 tcagagttga acggcaggct cacgacctct tggtataaac ggtgttcaaa agccagtttc
    13141 tcggtattaa gccaaattta tgcaaaaatg tgaaaagcca ttttcacaaa atttttctta
    13201 atactcgaaa ctaaacagct ttttcacaac cccctcagaa agtctattaa aattcattca
    13261 caaagaaatc gaataagcgc tgttcagatg gatgtacacg gtgggtaagt tccggatgcc
    13321 attgaacacc gaggatttta tgcatatcat ctttgctttg aaccgcttca acgagtccat
    13381 cgttagaaaa agcgatggct tttaataaag gtgaaagatc tttgactgcc tgatgatggt
    13441 aagaattcac aaaatatgga tcggggagaa ggttgcttaa caaactgtct gaaacaatct
    13501 tgacttcgtg agtcgtaaat tgcggggcag tgggctgttg ttcatgtttt actgaccact
    13561 ttggatataa tgaaagatct tgataaagtg taccttccaa aacgacattc aatagttgga
    13621 gtccccggca gactgcaaaa attggttttc tttgtttcaa tgcttccaag atcagtgcct
    13681 gttcaaattg gtctcgttga atatttgttt ccgttagctt tggatgagga tcttccatgt
    13741 aaagttgtgg actgacatct tgaccacctg caagaataag tttgtcgatt tgtccaatca
    13801 gttgaggagc tgaatctgga gacatgactg gcagaataag aggcaatcct tgagcatcta
    13861 aaactgcatc cacgaatcct tgcggtgtat agctcacttg atttccttgg aaagtatctg
    13921 ttgctcgaat cagttgattt ccggcaatgc cgatgattgg tttcatttgt attcccccta
    13981 aagtagactt attgtaacaa ggatttaaaa aaaattaaac tatgtaagac ttgcccctat
    14041 acttatctgt tcttgctgtg ttactatctg taaagagaaa tttcgtgtga aattcatgac
    14101 gatagttgaa aatattgctt aatttgttat aataaaaagt aagcagaaat aaaaaggttg
    14161 ggcgtaagct tcaaggatct agttcttcaa ggggtgagaa ggacatttta ttgaaggggc
    14221 ctgcctatac taagttatct ttagaggtac atactttcaa tgaaaaaaga aagcatgtac
    14281 ctctttttta aatcgaaagg aagtgaagca agtgttagaa gctttggaac aaatccgaaa
    14341 agcagaagaa gaaaatgaaa agcgtgctca aacactgcgg cagcagctga agatctatgc
    14401 aacagaaaaa gagcaggaat tggaaacgat ccaaacaaac tatcgacaac aactaaaaca
    14461 gttggtggaa gaaaaagaac aatttgaaat acaagaatta gaaaaagaaa aaatgaaatt
    14521 gaaagcagaa gcagacaagg tgcaagcaac tttgaaagat caatatcaaa agcatcagag
    14581 tcaagcgatc ggtgcgatca ttgaaagggt gaaagaaact tatggccgtc actaagatgg
    14641 aaaaagtgac cctcatttca gataaaagaa atcaggagag gattttacaa gcagtacaag
    14701 gaatccaaaa cgttgaaatc cgggatctgt atcaaaatag aacaaacaac cagtgggtgg
    14761 agaagtattt tccagatacc acgattattg ataaagaagc aaaattaagc gctttaggta
    14821 atcggttgaa agacatccaa gaagctatcc aattcataca acaccacggc gatagccgtc
    14881 aaaagaaact ccatttgaaa cgtcaagaac tttccttaca agatttggaa gccaattatt
    14941 ccgaagaaaa tttcataaat aaattgaaag aagtccttga tttaaaagaa aagtgggaac
    15001 aactaactga agaacgtgat cgtttagaag aagaggaaga atggcttctt cattggcaac
    15061 agctggatat tgctccaagt atgtatgcaa gtaagacgac catttttgag atggggactg
    15121 tgagtgcagc aaattttgaa agtttcaagg aagaactgag taagcttgat gacgtttata
    15181 tggaagaaat agattatacg ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat
    15241 cagttgctgt tatagaagaa attgctggga aatacgggtt caataaagaa acgtatcctt
    15301 atgaacaaaa tcctaaagaa aaactgcaag agaccaagca gcaattatcc attgttcacg
    15361 agcagcaaaa gaagataacg acagcgttag gtcagtgcag tggctatatc caagaatttg
    15421 aatgggtaga agaagtcaca cttgccgttg cagaacgaga aaaaataaaa gaacggttta
    15481 tccatgcgac ttatctgatc gttttgcaag gttgggtaga tgcagaagaa aaacagaatt
    15541 tgctgcatgt gttaaacgaa accgtatcag aagaagatat atacgtcagt ttcgaaacac
    15601 caaactcaga agagattcag caggaagtcc ctactaaatt gaaaaaccat ccgcttgtcg
    15661 aaccttttga attgctgact gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa
    15721 caccttggat gacaccattt tatcttgtct tcttcgggat gatggtcgca gatgttggtt
    15781 atggtttgct gatgctcatt gggacaatac tagctcaaaa actacttgtt ctaccacgcg
    15841 ggatgaaacg tttcgtaaaa ttctttgaga ttctctcgat accatcgatc atctggggat
    15901 tgatctacag ttcatttttc ggacagtcat taccaaaaga actgtttggc atccgcttgc
    15961 ctttcccaat cctatctacg acggatgacg tcaatacgat tttgatttta tccgtgattt
    16021 tcggtctgat acagattttg gttggattat ttgtggcagc aaaggaaaat atcaggagaa
    16081 aagattactt gagtgctgtc agtgatggat ttgcctggca agggatccta attgggatcg
    16141 ttattgcgtt ggttggtgcc atgttgataa aaaatacagc atttgtttac cttggcgctg
    16201 ggctatcgat cattgctgcc ttatgtatct tgatcgttcc gattatccaa acgccgtcta
    16261 aagtcaaagg tgctgccaaa ggtgcgtata atctttacgg actgaccggc tatatcggtg
    16321 atttagtcag ttatacacgt ttgatggcat tgggtatttc aggcggaagt attggagcgg
    16381 cattcaatat gctagttgct ttcatgccgc cagctgctcg attcagtgta gggatccttt
    16441 tgatcatcgc tttacacgca ctgaatatgt ttttgacact gctaagtgcc tatgtccatg
    16501 gcgcacgatt gcagtatgtc gaattttttg ggaagtttta tacaggaggc ggacgtgcct
    16561 ttgatccgct gaaaacggcg gaaaaatacg tcaacatcaa tcacaagaaa aaaaacaata
    16621 aataattgga ggatttttat ataatgatgg attacttaat tagtcaaaat ggtggaatgg
    16681 tatttgcggt attagcgatg gcaacagcga cgattttttc agggatcggt tcagctaaag
    16741 gtgttggaat gacaggtgag gcagcagcag cattgacaac aagtcaaccc gaaaaattcg
    16801 ggcaggctct gattctgcaa ttgcttccag gcacgcaagg tttgtatgga ttcgttattg
    16861 cctttttgat ttttatcaac ttaggaaatg acatgtcagt tgtacaagga ctgaactttc
    16921 taggtgcttc tctgccgatt gcttttacag gcttgttttc aggtattgcc caaggaaaag
    16981 tcgcagcggc aggtattcaa attttagcga aaaaacctga acatgctaca aaaggaatca
    17041 tttttgctgc gatggttgaa acatacgcga ttttaggttt cgttatttcc ttcctattag
    17101 tgttgaatgc ctaagcataa aggaggaaaa gcagatgaac gccattgaaa aaatcatctc
    17161 tcagatgaac gaagcagcag agcaggaaag agcggcgtta gaacaagaag agcgaatgaa
    17221 gatcgatcaa aactttgaac aaaaacgtac gcaagttgaa acagaacatc aaaaacaaaa
    17281 agaaaaacaa atcgaattac tcgaaaaaaa ataccgtcag ctaaggaatc gccagcaagt
    17341 agaagtacga caagaaaatt tgaatgcgaa gcaggaattt ttaagacgat tgtttgcaga
    17401 tgcggtaact gaaatggaaa attgggatga atctgaacaa atccagttta tcaaaaatgc
    17461 attgtattca ttacctttga caggaaaagt agcattcatt gctggagaaa aatcagcagc
    17521 ctatctttct cagacgttgt tggatgaatg gaacaatgaa cttcccttta tgatggtttt
    17581 aagtgatgaa actgttgctg atcaagcggg ttttctgata aacgatcaag gagttcagta
    17641 taattttctt ttcagcagcc ttgtacaaga tatccaagga acgatgagtt ttgagattgc
    17701 caatcaatta ttcgaataag gagggatcag tgtatggaat accacgaact aaatcccttg
    17761 attcgcggaa gagagcttga acttttgtcc aatgagacat ttgaacggat gattcaaaca
    17821 aattctgtcg aagcacttgg tgaaatattg aaatccacga tttacagtcc atatatttat
    17881 gacggatatg aatatgactt cgaacagaat cttttaaagg agcagagccg tctatttcgc
    17941 tggttaaagg aatcagcacc ggagcctgaa atcgtttgga tctatacgat gcgttatact
    18001 ttccataatc tgaaggtttt gacaaaagct gagatgacaa atcaaaatct ggaccatttg
    18061 tatatcgacg atggatttta ttcattagaa accttgaagg atgccattca tacgcaagtt
    18121 tcaaatgaac ttcctgaaag cattatggac agtatacgag aagtacgtga ttattttgaa
    18181 gagtctacca ttcttcaggg aattgacgtg atttatgatc gacagttttt gacagagcag
    18241 cgacggcttg ccgataagct aggatatcaa gaattgctga cagaagtcat tgcttttatt
    18301 gacttgacca atatcacaac gatggctcga ggaatattgc agaaaagaac atatgggttc
    18361 atgacgacgg tactttccag ctcgggaagt atcgaaaaag aagtattttt gaactttgta
    18421 gataaagata tggaagatta cacgaatttt ctattagaga cagattatgc tgattttctg
    18481 cgccctgtca tccgtgaggg aaatattgat ctcgtaaagc ttgaacagtt gaaagatgat
    18541 tatctaagtt cattgtatca agaagcacaa acccaggctt tcggaccact gccactacta
    18601 gcctttttga acgcaaaaga ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa
    18661 agaagccatt tcgaatcaga aacaatcaga aagcgggtga gacaagtcta tgacgcataa
    18721 gatcggtgta gttggcgaca aagattctgt tttgccattc aaattgtttg gctttgatgt
    18781 tcgttatggt acaaccaagc aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga
    18841 gtatggcgtg atttatgtca cagagcaatg tgcagcattg agcccagaaa cgatcgaacg
    18901 ttacaaggaa caaatgatac ctgctatcgt attgattccc aatcatcaag gatcattagg
    18961 gatagggctg gaagaaattc aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata
    19021 gtatgaaaag gagatagaaa ttttgcaaat tggaaaaatc ataaaagtct ccggacctct
    19081 tgtaatggct gagaacatgt ctgatgccag tatccaagac atgtgtctag tgggagatct
    19141 aggagttatc ggagaaatta tcgagatgcg tggcgatgtg gcgtctattc aagtatatga
    19201 agagacttct ggaattggtc caggcgaacc ggttcgctct actggtgaag ctttatctgt
    19261 tgaattagga ccagggatca tttcacaaat gtttgacgga atccaacggc cattagatac
    19321 ctttatggaa atcacacaaa gtaatttctt agggcgcgga gttcaattgc ccgcattgga
    19381 ccatgagaaa aaatggtggt tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg
    19441 agatgttatc gggatcgtag aagaaacaaa agttataaaa cataaaatca tggtgccaaa
    19501 tgggataaaa ggaacgatca aaacaatcga aaaaggttca ttcactattg atgaaacagt
    19561 atgtgtcatt gaaaccgaaa atggtgacaa aaatttgacg atgcttcaaa aatggccagt
    19621 aagacgtagt cggccaatca aagaaaaatt aaacccggat gcacccatga tcactggaca
    19681 gcgagtgatt gatacattct tcccagtgac aaaaggagga gcagcagctg tacctggtcc
    19741 ttttggtgca gggaaaacag tcgttcagca tcaaattgca aaatgggccg atgtggacat
    19801 ggttgtctat gtgggatgtg gcgagcgtgg aaatgagatg acggatgttt taaatgaatt
    19861 ccctgaattg attgacccta atacaggaga atcgttgatg gaacgtacgg tattgattgc
    19921 taatacatca aacatgccag tagcagctcg agaagcttct atctatacgg gaatcacaat
    19981 tgcggaatat ttccgtgaca tgggttatga cgttgctatc atggcagact ccacttcacg
    20041 atgggcagaa gcacttcgtg agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg
    20101 ttatccagct tatcttggtt cacggcttgc ggaatactat gaacgatctg gaagagtcat
    20161 tgcattagga tcagaaggtc gcgaaggaag tataaccgca atcagtgctg tatccccgtc
    20221 tggaggggat atttcggaac cagtcacaca aaatacattg cgggtcgtaa aagtattctg
    20281 gggtcttgat tccagtttag cccaaaaaag acattttcct tcgatcgatt ggatacaaag
    20341 ctattctctt tattcaacag aagtagggaa gtatatggat catatccttc aagctgactg
    20401 ggcatccatg gtcaccgagg gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat
    20461 cgtgcgtttg gtagggattg attccctttc agataatgac cgtctgacat tagaagtggc
    20521 aaaatccatt cgtgaagatt atttgcagca aaatgctttt gatgatgtcg atacattcac
    20581 ttcaagagag aaacaattca aaatgttgaa gatgatcttg acctttggtg aagaaggacg
    20641 ccgtgcgttg tcgttaggtg cttatttaag tgagatcatg gacggtaccg tatcgattcg
    20701 tgaaaggatc ggacgaagca aatatatccc ggaaacagaa ttagaaaaac tagatcaaat
    20761 tattggtgaa atcaagcaaa cgatgcaaac gatcgtttca gaaggaggca tgagcaatga
    20821 ttaaagaata tcgtacgatc ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg
    20881 ctggtgtcaa atatgaagaa ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg
    20941 gacaagtatt ggaagtccaa gaagataaag caatggtcca aatattcgaa ggaacaagtg
    21001 gtatcaattt acgtgactct tctgtacgat ttttaggcca tcctttagaa ctaggtgtat
    21061 cggaagacat gatcggtcga gtattcgatg gtttgggacg accgaaagat aacggaccag
    21121 agatcctgcc tgaaaagcat ttggatatta acggagaagt aatcaatcca gtagctcggg
    21181 attatccaga tgagtttatc caaacaggta tctctgcaat cgatcatttg aatacattag
    21241 ttcgtgggca aaaattacca gtattttctg gctctggtct tcctcataaa gaattagctg
    21301 ctcagatcgc gcgacaagca acggtactcg attcatcaga tgatttcgcc gtagtctttg
    21361 ctgctattgg aatcactttt gaagaagctg aatttttcat ggaagatttt cgtcaaacgg
    21421 gcgcaatcga ccgttctgtc atgtttatga atttagcaaa cgaccctgca atcgaacgta
    21481 tcgctactcc tcgtatggct ttgactgctg cagaatatct tgcttatgaa aagggaatgc
    21541 atgttttggt tatcatgaca gacatgacca actatgcaga ggcattgcga gagatttctg
    21601 cagcacggcg agaagttcca ggaagaagag gatatcctgg gtatctttac acgaacttag
    21661 ctactttatt cgaacgagca ggaagaatcc gcggactaaa aggatcggtt actcaaattc
    21721 cgattctaac aatgccagaa gacgataaaa ctcatccaat ccctgacttg actggttata
    21781 ttacagaagg acagatcatc ttgactagag agctttacaa gagtgggatt cagccaccaa
    21841 ttgatgtact cccgtctctt tcgcggttaa aagataaggg aactggaccc ggaaagacaa
    21901 gagaagacca tgcagctacg atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg
    21961 caaaagaatt agccgtagtt ttaggtgaat ctgctttatc tgatgtagac aagatctatg
    22021 cgaagtttgc tgagcggttt gaaaaagaat atgtcaatca aggattctac acgaaccgtt
    22081 cgattactga aacacttgat ctagggtggg aactgctatc gatgttgcca agaaccgaat
    22141 taaaacgaat caaagacgac atgctggaca aatatctgcc agaagggaag tgagttctga
    22201 tggcacgatt gaatgtgaat ccgacccgca tggaattgac tcgattgaaa aaacagttga
    22261 caactgccac aagagggcat aaattattaa aagataagca agatgaactg atgcgtcaat
    22321 tcatcctgtt gattcgaaag aacaaccagc tgcgtcaaga aatggaagag tctatgacag
    22381 aagcaatgtc tgattttgta ttggcaaatg cttcgatcac ggaacctttc atcgaagaat
    22441 tatttgtttt gccggcggaa aacgttgatt tgtctatcgt agacaaaaat atcatgagtg
    22501 taaaagtacc tgtgatgaat tttgaatatg atgaacagct gacagacaca cctttggaat
    22561 acgggtattt gaattccagt gccgaactgg atcggtcaat cgatcggttt actaccctgc
    22621 ttccaaaact tttggaactg acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa
    22681 tcgaaaagac acgtcggcga gtcaatgcat tagaatatat gacgatccct caattagaag
    22741 aaacgatcta ttatattcgt atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt
    22801 tgatcaaagt aaaaaatatg ggtaccactg actaagtggt atcttttttt aaaagaggaa
    22861 aacgatgatt aaacaaagca aacaaataaa gaaacgtttg ccggctgttc agcttctcgc
    22921 attaggattt tttctcctga ttttagttgg cggcggccta cttaccctac cattttttag
    22981 taatagtggg catgggacaa attttatcga tgcgcttttc actgcaactt ctgctgtctg
    23041 tgtaacggga ttgacaacat tgaatacagc agagcattgg aacagtgctg gtcaattttt
    23101 gatcatgctt ttgattgaga tcggcggtct tggttttatg atggttccga ttctatgctt
    23161 tgcattgata aagaagaaag tcagtctcag cacacgtatt atattaaaag aagcgctgaa
    23221 tctagaagaa gtatcaggtg tgatgaagct catgctttac atcctaaaat tggcctttgt
    23281 gatccaattg attggtacgg ttgctttatc ttttgtattt gtgcctgaat ttggctggtt
    23341 gaaaggtatc tggtacagtt tgttccatgc aatctctagt ttttgtaatg cagggtttga
    23401 tttgttagga gacagtttag ccaatcacca gcaaaatgtt tatttgatca tggtggtatc
    23461 tgctttgatc atttctggtg ggttaggctt catcgtatgg cgtgatttac tggaatatca
    23521 tcagaaaaag aagatgacga ttcattcaaa aatcgcatta accgttacag gtaccctttt
    23581 attaggcgga ttcgttgtgt tctttttcac agagcgcaat ggggcaacgc tagtggaagg
    23641 gaatatgttt caacgattgg caaacacctt ttttatgagt gtgacaccta gaacggctgg
    23701 atattattca gttgattacc tccatatgag ccatgcaggt ctgattttaa cgatgttttt
    23761 gatgtacatc ggcggaacat ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg
    23821 tgtgttgctg atccagatgc gttcgatgtt gaaaggacgt acgagagcgg aagcatttgg
    23881 ccgtaccatc cgacaaggcg cagtgttgcg ggcacttact ttattttttg ttacgttgac
    23941 gttgtgtgta gtcgccatca tgattttatc ggtgactgaa acgataccta aaacttctgg
    24001 catcgaatat attgcttttg aggtcttctc tgcgtttggt acagtaggtc ttacgatggg
    24061 attgacgcca gacttgactg caatagggaa attgatcatt atagcgctta tgtacattgg
    24121 acgagttggt gtattgactg tggtcttctc tctgatgaca aaagcacaaa gaacacaatc
    24181 aaaatacaaa tatccagaag aaagtgtcat gattggataa tttcatcttc tgacagaaac
    24241 ctagtgattt agtaaaataa gaaaataaaa taatttttct tgcttcttgg atactttaag
    24301 tgcaaaatgt tcgtttattt tttgaggcac tacccgttgt tgaacctcgg gtagtgcctc
    24361 ttttttattg aaaggaatca ataagatttg attcctttat ttatcaatag gttataattc
    24421 atattttttt attttttctt ttacgatact tagtccagaa gctaaaattt caaggttatt
    24481 agaggatagg gaaattcgaa cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga
    24541 atgataaagt cggataccgt tagcaagaaa gtcattctca attatcttag ggtcaatctc
    24601 aggatcaact ggaagaattc gatagtaagg gacacgattc gttggttttt ctactttgaa
    24661 aagagaatca aaaagctgat tggctctttt tgtaagactt aatttagttt ctaagatcct
    24721 atttgcttca tttgaatgga ggatttgagt gatgacttcc acatctaatc cagaagtttt
    24781 cacatttata ttaaaaattg cttgttctat cttgctttgg acatgtactg gaactacaag
    24841 ataagcaact cgcaatccag aacaaataaa ttttgtcatt ccagctaagt agattgtttg
    24901 gttaggtaat aaattttgga aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa
    24961 tgaatggata tcatcttcaa taacccatag accttctttt tcaatcactt cggctaattg
    25021 ctctcgtctt ttgtgagaga tttgaaaccc tataggatta ttacaagaag gcataagaaa
    25081 gacaccatga atcttctttt ttctacattc ctgaatcaat atatcaggtc gcattccttg
    25141 ctcgtcaaaa tcaattggaa cgatctctaa atgatataac tgagccaatt caatgaaatt
    25201 agcgtatgta tatcggtctg ttgctattct atcacctgga gagaaaagtg cagaaagtgt
    25261 gatagccaat ccattttgaa caccggaaac aatagataga gattctaagg acggaatgac
    25321 tccttgagtt tttaaccaat ttgctgcagt agtcaattga aaatttgtgc ctttgggatc
    25381 ttggtaatta agcaaaccaa caagatttgg gttattacta gttgattgaa taaacgggac
    25441 aacgaattta ttacattctt caaaagaact aaccagacca aaatcaatga tatgttcggt
    25501 tacttcattg gtagagatgg tgatggactc aaagacattg ggagaaataa atgtgccact
    25561 tccaatattc gtatacagca aacctttctc aattccatat ttataagcct tgccaacggt
    25621 agtaaaattt aaatctaaat aatcagctag ctctctttgg gagggtaact gagtgttttt
    25681 ctgtaattta ttctttaaaa tatcttgttc taattgttga atcaacgatc gataataagg
    25741 acgcttcaat ttttgtttgt ctggtcgcca agttaacggg taattgtcat aagaattaat
    25801 aggcattttc tccactcctt aaaattgtat accatacaat tttaccattg attgtattga
    25861 ttttagaagt taatcttttg tgaggaggga attgtctatg aaaattatta attttgagga
    25921 agtagtagct tcttttagtt ttgaagaagc gattcaagta atgaaaaaat gtttttttga
    25981 ttatgaaagt ggtaaaatca gccaaaatcc tcgattggtg gaaattttac ctgacggaaa
    26041 aggcaaaaat atgtttgcga tgatgcctgc ttatttgggg gagaacagat attttggcac
    26101 aaaagtcatt accgcttttc cggataatca tcagtatcat ttaccttctc atttaggaga
    26161 gattttcttg tttgatgctc aaaacggact acccaaagca ttgataaacg caaatgcagt
    26221 cacctggatt cgaacagctg ctgtatctgc gctggcaaca gattacttag caaatccgca
    26281 tgcagaaaca cttgctttaa ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat
    26341 ttctaccatt cgtcccatca ctacagtgtt tgtttacgat attttagaag aaagacgaaa
    26401 tacttttatc gctaaagcta aaaaacagta tcctaaaata acgtttataa atgcaatatc
    26461 agtagaagat gcagtacgtc cagcggaaat catttgtacg ttgacctcta gtaaagaggc
    26521 atttcttcaa aaagagtggg taagggaaga cgcacatatc aatgctgtgg gcacatttac
    26581 tcctgataca agagagataa gtacatcgtt gatggaaact tctcaaatct atgtagatga
    26641 ttactcagct gccttaacag aaagcgggga cctgcttata gccatggcag aaggagattt
    26701 agaggaatct tgtattgttg gtacattagg cgaattagtt tctgagaaaa aaacatttta
    26761 taaaggagaa tcgaggccga cgattttcga cgcagttggt ctagctattg aagatctttg
    26821 ttgtgcagaa tatatttata acaaattaga gaaaggggct ttaaaatgaa cttagattat
    26881 tacgtagttg atgcatttgc agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg
    26941 ctagaagaat ggctgccaga aggtacgatg caaaaaattg ccattgaaaa caatttatct
    27001 gaaacagcat ttacagtgaa gaaaaaccaa gaatttgagc ttagatggtt tacgccagat
    27061 cgagaaatcg atctttgtgg tcacgcgaca ttagcaacag catttgtttt atttaactat
    27121 tacaaaatac ctgatgaaac gattaaattt tctactcaaa gcggtaatct atttgtgaca
    27181 agaattaaag attattatta catggatttt cctagtatta tgccgaaaaa agtgccgata
    27241 ttgtctgaat atgaagaagc gattggggct aagattaaag aagcctactt agcacgtgat
    27301 ttatttttcg tattagaaga tgaaaaaacg gtggctaaat tgcagccaga ttttacagct
    27361 atcaaaaatt ttgaattagg aataggagtc atcgtgacag cagaaagtgt gcaaaaagac
    27421 tttgtttcta gaaccttctt tccaaaatta acaatcaatg aagatccagt atgtggatca
    27481 gcacattcta atcttattcc ttattgggca gaaagaatga ataaaacaaa attgtcagct
    27541 tatcaaatgt catcaagggg cggggatcta gaatgtgaat taaaagaaga tcgtgtgata
    27601 ataggaggaa aagcagtttt gttttctaaa ggtacctcgt ttatttgatt tctttcaaat
    27661 gaattaaaaa taaattggtg gagagtggca accttatagc tacaatggtt gttaaaaaaa
    27721 gacgcaaact ttttcaattt aatgaaaagt ttgcgtctca ttttaattat tggtgtttgt
    27781 tttttattca atcaagagta aaatgcccca tactaactat cctcggatga cttcgatttt
    27841 ataaccatca gggtcaatga caaagtagta agaaggcgca gtgccaggta atccttttaa
    27901 gtcagtaatc tcaagaccag cagctttatg ttgctcgtgt aatttttcta gatcatcagc
    27961 tgctatagcg atatgaccat agccgtctcc tagatcatac gcaccatgat catagttgta
    28021 agtcaactct aattcatagt catccccagg aagtgtcaaa taaacaagtg taaatttgtt
    28081 ttctggaaaa tcacggcggc ggctttcttc aaaaccaaat gccttttgat aaaattcgat
    28141 tgatgcttca agatctttca ctcgaacaca tgtgtgagcc attttcatag attcatctct
    28201 ccttttcctt ttactcaatc ataacaaaaa gaagtaagga aatagttgat ttgtgcttgt
    28261 tcatgagaaa tttctcatgt aaaatggata gaaaataaaa ctgttaaggt ggaaactatt
    28321 catggataaa ccaatttttg gtgaaaaaaa gcaatttgct gattatcaaa cccgttatgc
    28381 agcatatatt gtcatagaaa gagatcagca gattgcagta atcgaggctc caaatggtgc
    28441 tttttttctt cctggcgggg aaattgaagg agaagagacg aaagaagaag cgattgtccg
    28501 tgagctgata gaagaaatgg gaattgtggc agaaatttct tcttatctgg gagaagctga
    28561 tgagtatttt tactcgaatt atcgtgcaac ttattattat aatccaggat atttctttgt
    28621 tgctgattct tggaagcgta taggagaacc aactgaaaag accaataaaa tttggtgggt
    28681 gagccctcaa gaagcgcttg tgaaattgaa acgtggtagc caccgatggg cagtcgagag
    28741 atggatcgaa aaaaataaaa actgaaagaa agcgagtaga tcagctttct ctcagttttt
    28801 ttatttatta cagtacatat tttttgattg cttcagctac accgtgctcg tcgtttgatg
    28861 aagtgatgac atttgctgct tctttgacaa gagaaacggc atttcccatt gctactccta
    28921 gaccagcata ttcaatcatg gataagtcgt tctcgttatc tccgatagcc atgatttcat
    28981 cttgatcaat accaaggtgc tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt
    29041 tatttagtac ttcgaagtaa tagggcgcac ttttgaccgt tgtgtattta tcacggaaag
    29101 tttgcggtaa acgcgcaatt gctgcatcta aaatttctgg ttcgtcgatc atcatcattt
    29161 tgacgatgtt catgtctggt gtcatttctt caggtgttcg atattttagc ggcatcttga
    29221 ctaaacttgc ttcgtggatc gtataagcgc tgatatctcg attaggcgtg tagatcgctt
    29281 tgtcatcgat cgaatggaga tgactgccga cacggcgtgc cattacttcg atctctagga
    29341 aatcttcatg tgtcagtccg tgacgggcga tgattttacc tgatttcgtt tgttgtacga
    29401 gtgccccgtt ataagtaata acgtaatcat tatcttgaaa aaggttcaac tcgtttaact
    29461 gttcctggac acctggtaag ggacggccag tgcataaaac gatcgaaaca ccttttgcaa
    29521 cagcagcggc aatcgcttct tttacttctt ttgttacttc tcttttctca tttaataaag
    29581 taccatcgat gtcgatagcg actagtttga tagacataat ttcctccaat atattttaat
    29641 ttatttcaat caatgcgcca ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa
    29701 ataaatcata atgatcacgc aaggacggat caaccatttc ttttggaaag aaaaatcgct
    29761 catctccttg ttcttgtccc gctaatgcag caacaagctg actaacctgt gataattcta
    29821 tcaaagttcc gtcatttcgc aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc
    29881 gataaaaatc ataaggtaga tcgtagctag aattcacagc agtgtaataa acaggattat
    29941 atccgacctt ttctactaac aaagtgagtt cttggactaa ttttgaatcc cggttgccag
    30001 aaaaagtagc agatttcagt ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa
    30061 taggatcagc agattcagac cattgagtaa agtaagtgcc caagacccca tcatcaagtt
    30121 tgaggtattc ttctaaagta aagcttccgt caagaaatgg cagaagtaat tgagaatgga
    30181 gttcaaaact gctggcatct tcttgataca actcgtgtgc gcgatgaagc agatgctcca
    30241 aaattacttc cataccacgg gaaacaggat ggaagtacac ttgcacatac atttgatatc
    30301 ggctaacgat ataatcttcc actgcatgca tgccactcat ggaaaaagcg atcccgcctt
    30361 cataaggacg aatcacacgt aatattcggg taagatcaaa tgttccatat tctgttccag
    30421 tgaaataagc atcccgaagc aaatagtcca ttcgatctgc atcgatctga ctagaaatca
    30481 tttggacaac ttgtgggttg gggtaagttt tttggatcac actagctacc ttttcaggaa
    30541 aaccttcttc tactcggtta agaatctgat acacttctgt ttctggagaa gtgatgatct
    30601 ctacagtgat ggcttcatga ttcgtatgga agatatgttc aaatgtatga gagtaaggac
    30661 catgaccgac atcatgcaac agtgccgcac ataaagtcac cagccgttcc tgatcatccc
    30721 agcctccgtt gcctattttt tcttttgaaa aatttcgaga aaagatatcg cagattcgtc
    30781 ttgaaatctc ataaacgcct aatgaatgag taaaacggct atgttctgct ccgtgaaaag
    30841 tgaaagaaga agtgcctaat tgtttaatgc gtcgtagacg ttggacttct ttcgaattga
    30901 tcaaatctaa gattacttga tgctggacat ggatatagtt atggactgga tcacgaaaaa
    30961 ctttttccat aggcagcatt tgatatttat aaggggttgt cataatgtag ctcctttatg
    31021 ttattacgtt gttccattct ctggatttgg ttgttccatt gctccagctg gatttctttt
    31081 tttagccagt cggtagaatc ctgtaaatca cttgcagaat acagcttttc aaaagctttt
    31141 gcaaatgctt ctttgacttc ttttacaaaa aggggttggt caagcagttc ttcaagtgtt
    31201 gccattgtag caggatccac tgcaggatag ccatttgtac caaagtcttc ttttaaagca
    31261 gattgataaa atcgcttcat gacttgccca cgtttttcct gattaccgtt gacacttaag
    31321 tacatcataa cagaaatccc atttttgatc cgacgctgag cgataccggc aaattttttg
    31381 ccatggatgc ttaaatcata agttccagga caataagaat gggtgatttc gaatgcttgt
    31441 atcggtgcct ttggaaaagc gtatttagtc aataggagca tttgctcgta agcgtcatct
    31501 atggttattt tttttgtgtc actttggggg aaaatcaaag aaatattcaa aattccttca
    31561 tctgatacga tccctaatcc acctgaattt ctgatcactg gagcgtaacc tgcatcttcc
    31621 agtgtttgga tcccgtcact tagaaaagga gttcgaacat ccttcatacc taagatgact
    31681 gtgcagtcat attgccagaa atgtaaaaag atttgctggt gttctttaga atattccgtt
    31741 aatatatctg ttaatgcaaa aggggaaaag tcgttttttt gataaatgtg ctgatctagt
    31801 cgaatcgcag ggttcattca accggctcct ttgtataaat tcatattttc tacaatatta
    31861 tactctaaaa tcaaagagtt gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg
    31921 ttgacaaaac aaaagaaact aggaacaatg tagctatgag aagtaaagga gaatgaataa
    31981 tggaattatc acaaggaaca ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag
    32041 gggaagtgca agatttttat tttgatttga aagggcaaat ggtgacaatt ggcgatacgc
    32101 tgtacatacg ttataaagag attcaggagg agacgggcga tgagattcct gtaacgatca
    32161 aattagttcc agatggtcat gtacagctga tccgtgcagg agaaatgcgg atgcgtttga
    32221 aatttggcta taaagaacgg ttagaaacat cctatcgaac accatacggt atgatccaga
    32281 ttgctacttt tacgaaagaa ctgcatgtta gtttgaaaga ccgtcccaca gcagggaagg
    32341 ttcgaatcga ttatgatctg ttcatgggac cggaaaaaat cggcgaatat tatttgacct
    32401 tggattttac agcgtaaata taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga
    32461 taaggagtag actgtgaaag gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa
    32521 acaaaagcga attatcaatg atcgaagtag cacacgcgat cttagaacaa cgtggcgatg
    32581 tcatggattt ttctgattta gctaaccaga tccaaaacta tcttgggaaa tcagacagtg
    32641 aaattcgtga ttcactcgca caattttata ctgacttgaa tattgacggc agctttatct
    32701 cattagggga taatcgttgg ggcttgcgtt catggtatgc tatcgattcg atcgatgaag
    32761 aagtaaacca tggaatggac gaagaggatg aagatactcc acgtcgccgt aaacgcaaga
    32821 aagtcaacgc ctttatcaat gatgatgaag acgcgatcga ttacaatgac gatgacccag
    32881 aagatacaga tctgacagaa gaagatgacg atgatctgtt tgatgacgat gatgatgaag
    32941 atgaagaaat cgctgcttat aattctgact tgcaagaaat cggagcagat gacccgacag
    33001 atgatgaaga agacttgcca ggaaatatcg aagaagattt aagtatcata gacgaagacg
    33061 atgatgatga agattatgaa gaggaagaat tttctgactc tgaaaaagaa taaacattat
    33121 tttcttacga gaggttgtga cagagacgat cagcctatca gaataaggag gtgtgacacg
    33181 atttttgtca caccttcttt tttgtatata aaaatcccaa gtaaatacat aaaaaataaa
    33241 aaaattgaaa cttttagcgc accttgtacg tcatattaag tgaggcagaa aggagtggag
    33301 aatgattttt actggatata agaaaaaacg gcagatcgaa aaataccgtc acctgctgaa
    33361 tggactcatc gaaactgatt atcaaaaaat gttccgtatc acgatgaatt acgtacatga
    33421 taaagaagag gccttagatg tgatgcaaga tagttttcat aaagccctga actattttga
    33481 aaaagaaaaa gagatcgagc attttagtgc atggttttat cggatattga tccgcacggc
    33541 attagatagt tggcgcagac aaaaaaggac gcctgtggac ttatttgaaa ttcaagatat
    33601 cccccaacag ttacctttag aaacttccgt tgcagagctc catatgattt tagataaaat
    33661 agacagtccg gaaaaagaga ttctgatcct ctactttttt gaaggatttc gtttgaaaga
    33721 aattgcgact atcttggata tgaatgaaaa cacagttaaa acgaaaatgt accgctcatt
    33781 gaatgcttta cgtcaaatcc tagaatgaag gagagaagag tgccatgtat tcccaaaaag
    33841 aaaatttaaa aaaactaaag aaaacttata aagaacaagc aattcccaaa gggcttaaag
    33901 aggagatccg ccagcgtttt attatcgaag agcaatcatt tataaaagca aagcggagaa
    33961 agagacgtct ccaagggttc ggagtatctt tggctatcgt aatgattaca acaagtagcc
    34021 ttctttttaa tagtcaagtt cgaagctttg cagaagattt acccatctta ggatcagtca
    34081 ttgaattgat cttaggcgaa cgctttactg atcgatctga aaaaatcgat attcaggtac
    34141 cgaagatcaa tacacaaaat gagaaagaaa ataaaacaat tcatggatta aatcaaaaat
    34201 actttcgcga aggacaagca gattttgaaa aagctgaaaa agaatatgga aattttgaaa
    34261 cggatcatta ccaagttttg ggagattatc agaaaatcgt agacgataac cggtttctcg
    34321 tgattgaaag gcagatcacc caaacagcag cagatagcca tgttgaaaaa agatacgaca
    34381 caatcgacaa gaaaaacagt gtccagctct cacttccact tttattcaaa gatgacacct
    34441 atctatctgt tttgacaaaa gaagtcaaac ggcagatggc cgaacaagtc aaggaagatc
    34501 catctaagta ctattggact gagcaagata ttcaagaagg gacgatagaa aaaccaacac
    34561 ttgttacgcc tacacgaagt ttttatttga acaaagagca tcaattagtt ttgacttttt
    34621 ctcaatacga aattgctcct ggttatatgg gtacaccaga atttgtcatt cctaagtccg
    34681 tgacaaaaac gattttggca tcggaagatt atttggatca ttagacgaga agtagttgcg
    34741 actagcagtt tcaattttca agtaagtaca agtaaataat aagaagagaa aaagagaaag
    34801 aagagatata gtgaaaagaa attatttagt tatcagtatt attgctttat ccgtgctttt
    34861 gtttttcgaa attacttcgg tggcctcctt gatcaccgaa gtaaaaggac aaacacactc
    34921 ttcggagcat tcgtcacaat ctttcaaaca atcagaacaa tcaaccatta aaagccaatc
    34981 aactgaaaca gctaacgtca aagaagccat tcaagaatca gcggaaaagg tacaaagtca
    35041 gtcatcacaa tcaacacaag acagtacaaa aacgagccga gatccggctg tcaaaagcgt
    35101 cgcaatcagc tttgatgatg gaccaggagc gaccacaaca ccgcagcttc tgcgaatctt
    35161 aaaagaaaaa aacgtgcatg ccactttttt cgtcttagga gaaaatacgg cgcagcatcc
    35221 tgaaatcgtg aagcagacag ctgaagcagg gcacgagatt ggcaatcaca catacgatca
    35281 tcagaatctg gccactcttt ctgcccagtc gatgacggag gaagtgacga aagcagacac
    35341 tgagatcaaa aaagtcactg gcaaaacccc cacttttgtt cgtccgccct atggttctgt
    35401 cacgaatgta ggagcaacga tcatccagcg accgatcatc gaatggtctg ttgattcaga
    35461 agattggaag acaagaaatc ctgatttgat tttgcagaag atccaagcaa cagtatacga
    35521 tggggcaatt attttattcc atgatatcta tcctgaaacg attcgtgccg ttcctcaagt
    35581 gatcgattat ctgaaagagc aagggtatcg catcacgaca gttggggatt tattagggca
    35641 tccaacggct gtcgagaact attatggcag aaacgatcat cggccagttc agtaagtgag
    35701 acaagtatct ttaataaata acacatttta ataagtagtt gaacacataa ctaccttact
    35761 tgcaaaggct taacaatatc tatacaggta caacaaaaaa ctgtccacca attttctttg
    35821 gcggacagtt ttcacttaaa aataaagaag caactattcg taaattatgc taaaatagca
    35881 gtagaacgcg tcctgaggga atcgaacccc cgtctcaaga accggaatct tacgtgatat
    35941 ccactacact aaggacgcaa gcactgggat aattgtaact aaacagataa aaaattacaa
    36001 ggggattgga caaaaaaatg aaatttgagc aaaatttttc acaaaaacag cagcagacac
    36061 agaaactagc catgacgcaa cagttgcaac agtccatcca agttttacaa tataacagtg
    36121 aggagctgtt ggcattcgtt gagaatcaag caatggaaaa tccattggtg gaagttgttg
    36181 agccggaatg gcagccagat tacataaaag cttcttcatc ttcttatgaa ggagaagaga
    36241 caaattacct aaatcagatt cctgatacaa aagggtcttt atttgattca ttgatcgaac
    36301 aagtccattt gaattaccga gatacttttt tgaggaaaat cgttctttat cttgtagaat
    36361 acatcgattt gaacggattt ttgacgatta gtttagaaga agccggaaaa gagattggag
    36421 ctacgcctat ccaaatgctc gatgcgttga ctctgattca gcaattagaa ccagctggag
    36481 taggtgcacg tagtttgcaa gaatgtctca tgcttcaaac agaacgagat gattatgcgc
    36541 cggaattagc ttatatcgtg ttggaagaat gttttgagga actggtggag cgaaaatgga
    36601 aagaaatcgc acaacgtttt tctgttgatc tacatgctgt tcagcagata ttcgactatt
    36661 tacaaacgtt gagtccaagc cctggccgga ttttcgaccg tagcagcgaa ttgtacatta
    36721 ttcctgatgt ccgggtatta gtggacgacg ataaaaatgt acaagttatc tcaaaccgaa
    36781 aaaatcagcc aaatatacgc tttcaagaga gttacttcaa acaaatgtca caacaagcag
    36841 ataaagaaac tgaaaattat ttaaaagaac ggaaacagga attcgaatgg ttgaagaaaa
    36901 cgatcctaca acgtggggat acaattttgc gggttgctca agtcattgtt tcccggcaaa
    36961 aagctttctt tatcgacaag gaacgtccaa tcaagcctct tacgttaaag gaagttgcaa
    37021 ctgagataga tgtgcatgaa tccacggtca gtcgtgcagt caacgggaaa tatttggaaa
    37081 cagacttcgg tgtatttgaa ttaaaaaaat ttttcactac acgaattcct acgaatagta
    37141 cagaacagac agaagacctt tcagcagata cagcgaagaa aaagctgcag gaattagtcg
    37201 atcaagaaga caaaaacaag ccgttgtctg accagaaatt ggttgaattg ctgaaaaaag
    37261 atgagatagc gatctcaaga cgaacagtag caaaatatcg tgatttatta ggaatcccga
    37321 gttcatcaaa aagaaaacgt tatgataact aatcagtcaa aagactagaa aaagtatttc
    37381 actttttcta gtctttttag ttgaaaaacg agagcaaaac tgttagactt acagtcgtga
    37441 ggttgagatt tccctttatt gatgtgaaat tcgaatagtt aatttctttt tttatcgtat
    37501 atgggtcgtt ttaagtctac attggacgta aagtgtccaa ctaaggagat cgctatgctg
    37561 gatgaaataa aaatgattga atctgttgca ccagatatta tcgaagtgtt gcaagaacgc
    37621 tataaaattt tacgaaatat ctattggatg cagccgattg gacgtcgaag tttatcggaa
    37681 agtatgggga tcacagaacg agtattgcga acagaaacag atcttttgaa aaatctgaat
    37741 ttgatcgaca cctcaaaaag cggtatgacg ctgacaacta agggcgaaga agtttatcaa
    37801 agccttgaaa acttcatgga tcagctttta ggtacgcatc aaaccgaaca acagttggca
    37861 gaatattttg gtaaccaacg ctgtatcgtt gtttcaggaa acagcgaaga gcagaccaaa
    37921 gtggctgatg cattcggcga agcgctgagc gaagccctag accgactact tccagaaggc
    37981 gagaatatca tcgctgtgat gggaggaact actatggctg tggtagccga acaattgtct
    38041 aatctggaaa ataagaaacg gcataacctg tttgtccctg caagaggcgg gattggtgaa
    38101 gcgataactg tacaggcaaa ttctgtcagt gcaagaatgg ctgctaaagc aaacggtaac
    38161 caccgtgcac tttatgttcc ggaacaatta agtctggcaa cttacaattc tttactcaat
    38221 gagccatcta tccaagaagt cttaaacttg attagtgaag cgaattgtgt tattcatagt
    38281 attgggcgtg cattacacat ggcagcacgt cgtaagatga ctgaaaaaga attagtcatg
    38341 ctgaaacaag caaatgctgt tgcggaatct tttggctact ttttcaatga aaaaggtgaa
    38401 gtcgtttata aggtccctag aatcggcata gaattgcaag atttggaaaa agtcccgatc
    38461 atcatggcaa tcgcgggcgg aaaatcaaaa gctaaagcaa ttcgagctta tatgaaaaat
    38521 gcaccaaaac aaacgtggct catcactgat gaagctgctg caaatgagat tttaaaaggg
    38581 gtaacccttt aaaataaata tttttttgat tttcataagg aggaaatctt aaatgacagt
    38641 taaagtaggt attaatggtt ttggacgtat tggacgtcta gcattccgtc gtatccaaga
    38701 tgtagatgga atcgaagtag tagcaatcaa cgacttgaca gatgctaaaa tgttggcaca
    38761 cttgttaaaa tacgacacaa ctcaaggacg tttcaacgga acagttgaag ttcatgaagg
    38821 ttcattcaac gtaaacggaa aagaagttaa agtattagct aaccgcaacc cagaagaatt
    38881 accatggggc gaactaggcg tagatatcgt tctagaatgt actggtttct tcacttctaa
    38941 atcagcagct gaaaaacatt taacagctgg tgctaaacgc gttgttatct ctgctcctgg
    39001 cggaaacgat gttccaacaa tcgtttataa cacaaaccac gaaacattaa ctggtaaaga
    39061 aacagttatc tcaggtgctt catgtactac taactgttta gctcctatgg ctaaaacatt
    39121 gaacgacaaa tttggtgttg ttgaaggatt aatgacaact atccacgctt acacaggtga
    39181 ccaaatgact ctagacggac ctcatcctaa aggtgacttc cgccgtgcac gcgctgctgc
    39241 tgcaaacatc gttcctaact caacaggtgc tgctaaagct atcggtttag taattcctga
    39301 attgaacggt aaattagacg gagctgctca acgtgttcct gtaccaacag gttcattaac
    39361 agaattagta acagttcttg aaaaagaagt aactgttgac gaaatcaatg cagcaatgaa
    39421 agaagcttca aacgaatctt acggatacaa cacagacgaa atcgtttctt cagatatcgt
    39481 tggtatgact tacggttcat tatttgatgc tacacaaact aaagtaatga cagttggcga
    39541 caaacaatta gttaaaactg ttgcttggta cgacaacgaa atgtcataca ctgcacaatt
    39601 agtacgtact ttagaatact tcgctaactt ataagataga caaacgtaca agtgtaaatt
    39661 atagacatgt acatttaaaa gcggggaagc aatcgcgctt cttccgcttt ttttgataat
    39721 aagtttatt
//
LOCUS       DASNNU010000015        39660 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.015, whole genome shotgun sequence.
ACCESSION   DASNNU010000015 DASNNU010000000
VERSION     DASNNU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.015"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..328)
                     /locus_tag="U8116_000727"
     CDS             complement(26..328)
                     /locus_tag="U8116_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN8433742.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="U8116_000728"
     CDS             complement(353..2446)
                     /locus_tag="U8116_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN8433743.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="U8116_000729"
     CDS             complement(2935..3699)
                     /locus_tag="U8116_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEN8433744.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="U8116_000730"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="U8116_000730"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEN8433745.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="U8116_000731"
     CDS             complement(4704..5780)
                     /locus_tag="U8116_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN8433746.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="U8116_000732"
     CDS             complement(5804..6397)
                     /locus_tag="U8116_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEN8433747.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="U8116_000733"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="U8116_000733"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEN8433748.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="U8116_000734"
     CDS             complement(8067..8366)
                     /locus_tag="U8116_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEN8433749.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="U8116_000735"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="U8116_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEN8433750.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="U8116_000736"
     CDS             complement(10121..11032)
                     /locus_tag="U8116_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN8433751.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="U8116_000737"
     CDS             11152..12009
                     /locus_tag="U8116_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN8433752.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="U8116_000738"
     CDS             complement(12196..12558)
                     /locus_tag="U8116_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEN8433753.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="U8116_000739"
     CDS             12904..14343
                     /locus_tag="U8116_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8433754.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="U8116_000740"
     CDS             complement(14350..16902)
                     /locus_tag="U8116_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEN8433755.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="U8116_000741"
     CDS             complement(17016..18347)
                     /locus_tag="U8116_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN8433756.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="U8116_000742"
     CDS             complement(18313..19791)
                     /locus_tag="U8116_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN8433757.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="U8116_000743"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="U8116_000743"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN8433758.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="U8116_000744"
     CDS             22401..23828
                     /locus_tag="U8116_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8433759.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="U8116_000745"
     CDS             complement(23920..24285)
                     /locus_tag="U8116_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN8433760.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="U8116_000746"
     CDS             complement(24282..24647)
                     /locus_tag="U8116_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN8433761.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="U8116_000747"
     CDS             complement(24839..25087)
                     /locus_tag="U8116_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433762.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="U8116_000748"
     CDS             25086..25214
                     /locus_tag="U8116_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEN8433763.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="U8116_000749"
     CDS             25271..26965
                     /locus_tag="U8116_000749"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEN8433764.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26969..27088
                     /locus_tag="U8116_000750"
                     /pseudo
     CDS             26969..27088
                     /locus_tag="U8116_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27065..27238
                     /locus_tag="U8116_000751"
     CDS             27065..27238
                     /locus_tag="U8116_000751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433765.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            27616..28749
                     /locus_tag="U8116_000752"
     CDS             27616..28749
                     /locus_tag="U8116_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEN8433766.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28780..29100
                     /locus_tag="U8116_000753"
     CDS             28780..29100
                     /locus_tag="U8116_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEN8433767.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29129..29593)
                     /locus_tag="U8116_000754"
     CDS             complement(29129..29593)
                     /locus_tag="U8116_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8433768.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29725..30978)
                     /locus_tag="U8116_000755"
     CDS             complement(29725..30978)
                     /locus_tag="U8116_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN8433769.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(31007..31324)
                     /locus_tag="U8116_000756"
     CDS             complement(31007..31324)
                     /locus_tag="U8116_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN8433770.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31350..31670)
                     /locus_tag="U8116_000757"
     CDS             complement(31350..31670)
                     /locus_tag="U8116_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN8433771.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32134..33549)
                     /locus_tag="U8116_000758"
     CDS             complement(32134..33549)
                     /locus_tag="U8116_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN8433772.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33566..34882)
                     /locus_tag="U8116_000759"
     CDS             complement(33566..34882)
                     /locus_tag="U8116_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN8433773.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35032..35730)
                     /locus_tag="U8116_000760"
     CDS             complement(35032..35730)
                     /locus_tag="U8116_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEN8433774.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36019..36975)
                     /locus_tag="U8116_000761"
     CDS             complement(36019..36975)
                     /locus_tag="U8116_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN8433775.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37088..37921)
                     /gene="msrA"
                     /locus_tag="U8116_000762"
     CDS             complement(37088..37921)
                     /gene="msrA"
                     /locus_tag="U8116_000762"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN8433776.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38096..>39660)
                     /locus_tag="U8116_000763"
     CDS             complement(38096..>39660)
                     /locus_tag="U8116_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433777.1"
                     /translation="FKNTQSIYTTGDLSFHLSRIKGLSTIFTSPINYETFNYTGYGVN
                     YFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNLCTTLVAYHYGERFLKQKKAAFLFS
                     CLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFYAYQIISGKEEKYPSVKLALSMSLL
                     VYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFIAIFKKLFSAAGMTLVLTSAFWYPM
                     FEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDLTTYSMGLVGLLSLCIPLILFKK
                     LTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLLQFPWRILSLQIIFSSLILTMIF
                     FKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTTKG
                     MDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLP
                     VYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAY
                     LCLFFKIVFHKR"
BASE COUNT        11999 a         8192 c         6732 g        12737 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
    27121 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
    27181 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
    27241 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
    27301 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
    27361 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
    27421 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
    27481 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
    27541 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
    27601 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
    27661 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
    27721 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
    27781 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
    27841 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
    27901 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
    27961 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
    28021 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
    28081 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
    28141 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
    28201 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
    28261 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
    28321 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
    28381 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
    28441 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
    28501 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
    28561 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
    28621 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
    28681 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
    28741 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
    28801 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
    28861 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
    28921 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
    28981 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
    29041 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
    29101 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
    29161 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
    29221 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
    29281 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
    29341 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
    29401 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
    29461 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
    29521 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
    29581 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
    29641 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
    29701 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
    29761 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
    29821 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
    29881 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
    29941 actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat
    30001 aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt
    30061 tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc
    30121 taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat
    30181 aaacggtgcg ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt
    30241 gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa
    30301 tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg
    30361 ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat
    30421 tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc
    30481 ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt catttccgct
    30541 taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt
    30601 tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc
    30661 ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa
    30721 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
    30781 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
    30841 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
    30901 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
    30961 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
    31021 gacgatacag atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat
    31081 gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
    31141 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
    31201 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
    31261 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
    31321 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
    31381 agtacttttt caccattcat catgccatag tcagccatat tgattacatc taaaggaatg
    31441 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
    31501 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
    31561 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
    31621 cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg
    31681 ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt
    31741 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
    31801 agcgcacact aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa
    31861 aaagttgtga cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc
    31921 atctgcggta ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt
    31981 cttattactt gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc
    32041 tgaccctggg tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag
    32101 taaatacaat gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc
    32161 caattctctc ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct
    32221 tcgttcatcg ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg
    32281 tgacacacag tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt
    32341 tgctttgatc aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac
    32401 gattcctttc tcatccatat actgttcttc atattctctt cccataccgt tttctgttat
    32461 cagccaaggg atattatgat aattttcttt tagatacatg gctatatcat acatgatttt
    32521 cggatagatt tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc
    32581 ataatatcgt tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac
    32641 gcgtttagga taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc
    32701 taaatcctct gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc
    32761 ttctgtgtag tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata
    32821 catataagca gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt
    32881 taaaatacag ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc
    32941 cgttgctaaa actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg
    33001 ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa
    33061 ccagtttttt accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa
    33121 atcaactact tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc
    33181 ataatgttcc aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc
    33241 acttatatgc tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg
    33301 agcccaattg atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc
    33361 ctgctggtat tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac
    33421 aaaatgttca tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt
    33481 ccaaccttcc gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt
    33541 aattttcatg atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt
    33601 ttacaaatgg cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg
    33661 caccgatatt tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga
    33721 ccattacaac tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag
    33781 tgataagagg cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac
    33841 caaaaataac tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt
    33901 gtttcatatc ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc
    33961 cagacccgcc catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt
    34021 cttttccagc ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc
    34081 gtatcggctt caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa
    34141 acatcaatag caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga
    34201 cattgtaaat gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag
    34261 ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat
    34321 tcgttggcac ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat
    34381 aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat
    34441 attgtttagc caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa
    34501 cagttggatt aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca
    34561 ctttattcag cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac
    34621 ttagtgtgac atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga
    34681 aacttatctg tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa
    34741 aagcacccgt aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat
    34801 accttaaatt attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa
    34861 aactcattcc agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta
    34921 ttagtcagcg cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat
    34981 aaaacaaaaa ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt
    35041 ttctttttct cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat
    35101 tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat
    35161 atcgatcgtc tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga
    35221 ttcattaatg attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat
    35281 cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc
    35341 aatcttttgc gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc
    35401 caaacctgcc atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg
    35461 atcctgatct tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact
    35521 gacaaatgtc cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac
    35581 agcttctcgc accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg
    35641 cagtttatct cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt
    35701 tacttctact agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt
    35761 ttttcgtaat tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat
    35821 taatccgttc atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
    35881 attcactcct agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa
    35941 cagacgatct tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa
    36001 agtttgatta catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga
    36061 gaaatctaaa ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc
    36121 gaccagtaca cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa
    36181 tgattgtcct cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc
    36241 aacttgttct tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat
    36301 accatgtaga taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc
    36361 tagcccttct tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat
    36421 attgtagcgc atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat
    36481 tttttttgta tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct
    36541 tacaagttgc atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg
    36601 atgatagaag atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga
    36661 aatgatggat ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc
    36721 tttgctagaa ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc
    36781 aaagtttttc tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt
    36841 aattcccatg tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc
    36901 accaaaattt tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga
    36961 tttacgatat tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc
    37021 tcatcaaatc tttttaaaac gctttctttt ttctattttt agtttactac attttaaaga
    37081 aaaggagtta atcaaaatga ttttttcgta tatttttttg ccatttgttt tttactcgtt
    37141 gatagatcag aaattgatta cgtgcttgct ggattttttt ataacgtttc ggttgttttt
    37201 tgtaaaattg ctgatgatag ttttctgctg gccaaaacgt accggctggt tcgatttttg
    37261 tgacgattgg ttggtggaaa gttcctgaac tgtctagttt ctgttttgat tgttccgcta
    37321 attctttttg tcgttcgact tgataaaaaa tcactggacg atattgcttc cctctatctt
    37381 gaaattgtcc aaatgtatct gtgggatcag taatttgcca atagattgtc agcaactcgc
    37441 tataacttat ttctcttgtg tcataaatga tctccacagc ttccacatga cctgtgtaac
    37501 caccacttac ttgatcataa tttggttttt caacatgtcc gcccgtgtat cctgacaaaa
    37561 cagataggat cccttttttc gattcaaacg gctcgaccat acaccagaaa catccccctg
    37621 caaatactgc acgttcttga tagtcagcat cttgcatggc atgcttggaa aagtcatatt
    37681 tttgctcctt atcaaatcct tccgtaatca ataaataaaa gtccattaca tcaggcgtca
    37741 gattcattcg gatagctaat ggacgtagtg ttgcttctag ttttgataac tgttctttaa
    37801 cggatatatt ttctcttatg tcattctttg tctgaatcaa tacttttcgt tcccaatctc
    37861 gtgtattcgg attcaaaatc agattataaa gtgtttgaat gaatatttgt tcctcatcca
    37921 tagattctcc tcctttccga agaagacaag ctagtgactt tagctgtaat ttatcataaa
    37981 gtgaggaaat aagcgattac ttcatccccc tacagatttt tcgtataaaa aagagagaat
    38041 ccttatcgac agattctcct ttttaatcaa tttattttat aaatgaaaag ttaatttacc
    38101 ttttatggaa aactatttta aaaaataaac ataaatatgc agttaatgta acaagataag
    38161 aaataatagt cgttactgta taacttgtgc ttactttaat atcagcaact cctttgactc
    38221 cgcttatttg taccaatcca ttagcattca cctgctcctt tacaggttga tgatttattt
    38281 ctacattcgt tcctaaataa gcatatacag gaagtgtgat tacttggctc ttctcattag
    38341 ttactctata tatgatagaa ctaccagttg ttttataagg aaggtttacc cattgactgc
    38401 caatctttgc ttcgtgtttt attatatgct ccttattctc caatgaagcg ataggagaat
    38461 aatcatataa aaaataatcc attcctttag tggtatacgc ctctactgtt tctttgttta
    38521 caacaatatg tcccggctga gaaattattt tttgattgaa atttttttta ctagaaacag
    38581 ttaaagtgat taataacaaa acagtcataa ataaataaaa tagttctctt gtcttattgt
    38641 atcttctatt tttgaaaaat atcatcgtta atatcagaga agagaatatt atttgcaagc
    38701 ttaagatccg ccaaggaaat tgtaacaatt tggctggcgt attttgcaga agataccatg
    38761 gcaccaaact agttgtagct agccaagtca aacacgtacc ataataaatc tttttttcta
    38821 tgtttgttaa ttttttaaat aaaatcaggg gaatacataa agataacaga ccaactagtc
    38881 ccattgagta agtagttaag tcgttgttca tagcttcagt cagactatcg aacacattag
    38941 atgcatgagc atataaattt gtcacgctag gtttgttgat tttttggtag agcatttgtt
    39001 caaacatcgg ataccaaaaa gcactcgtca gaaccaatgt cattcctgcc gcactaaata
    39061 attttttaaa tatagcaata aaatctgcat tcttgaaacc tttagaaacc aaacgaataa
    39121 aaataaaaat gataattaat gctgtactca ttaatgttga tagtacatgg ctgtacacta
    39181 gcaaactcat actcaatgct agctttacag acggatactt ttcttctttt ccagagatga
    39241 tttgataagc ataaaataat acagggataa caagtgttat tgcgatagct tcggcaattg
    39301 cggaacgata gtagatgtct actgtccgat aagcagagaa aatatagaga caactaaata
    39361 aaaatgccgc ttttttttgt tttaaaaacc gctcgccata atgataagct acgagtgttg
    39421 tacacaagtt caataaccaa acgtaaataa tatagcttac aatcaaattt tttgtcatcc
    39481 aatataacat tacagctgga aaaaaagtca gaaagggata aaaataattt acaccatatc
    39541 cagtataatt gaatgtctca taattaatcg gactggtaaa aatagtcgat aaccccttga
    39601 ttcgagataa gtgaaaagaa agatctccag tggtataaat actctgtgta tttttgaaaa
//
LOCUS       DASNNU010000016        39545 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.016, whole genome shotgun sequence.
ACCESSION   DASNNU010000016 DASNNU010000000
VERSION     DASNNU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.016"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..245)
                     /locus_tag="U8116_000764"
     CDS             complement(<1..245)
                     /locus_tag="U8116_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN8433778.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVI"
     gene            complement(361..927)
                     /locus_tag="U8116_000765"
     CDS             complement(361..927)
                     /locus_tag="U8116_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEN8433779.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(1063..1716)
                     /locus_tag="U8116_000766"
     CDS             complement(1063..1716)
                     /locus_tag="U8116_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433780.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(1766..2194)
                     /locus_tag="U8116_000767"
     CDS             complement(1766..2194)
                     /locus_tag="U8116_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN8433781.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(2199..2573)
                     /locus_tag="U8116_000768"
     CDS             complement(2199..2573)
                     /locus_tag="U8116_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8433782.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            2877..3062
                     /locus_tag="U8116_000769"
     CDS             2877..3062
                     /locus_tag="U8116_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8433783.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            3075..3323
                     /locus_tag="U8116_000770"
     CDS             3075..3323
                     /locus_tag="U8116_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433784.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            3354..3524
                     /locus_tag="U8116_000771"
     CDS             3354..3524
                     /locus_tag="U8116_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433785.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(3626..4084)
                     /locus_tag="U8116_000772"
     CDS             complement(3626..4084)
                     /locus_tag="U8116_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433786.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            4252..4569
                     /locus_tag="U8116_000773"
     CDS             4252..4569
                     /locus_tag="U8116_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433787.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            4502..4981
                     /locus_tag="U8116_000774"
     CDS             4502..4981
                     /locus_tag="U8116_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEN8433788.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            4998..5174
                     /locus_tag="U8116_000775"
     CDS             4998..5174
                     /locus_tag="U8116_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433789.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            5431..5862
                     /locus_tag="U8116_000776"
     CDS             5431..5862
                     /locus_tag="U8116_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433790.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            5868..6185
                     /locus_tag="U8116_000777"
     CDS             5868..6185
                     /locus_tag="U8116_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HEN8433791.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            6304..6990
                     /locus_tag="U8116_000778"
     CDS             6304..6990
                     /locus_tag="U8116_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN8433792.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            6965..8314
                     /locus_tag="U8116_000779"
     CDS             6965..8314
                     /locus_tag="U8116_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN8433793.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            8305..8778
                     /locus_tag="U8116_000780"
     CDS             8305..8778
                     /locus_tag="U8116_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN8433794.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            8753..9280
                     /locus_tag="U8116_000781"
     CDS             8753..9280
                     /locus_tag="U8116_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEN8433795.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            9295..11607
                     /locus_tag="U8116_000782"
     CDS             9295..11607
                     /locus_tag="U8116_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN8433796.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            11854..12171
                     /locus_tag="U8116_000783"
     CDS             11854..12171
                     /locus_tag="U8116_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEN8433797.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            12168..12329
                     /locus_tag="U8116_000784"
     CDS             12168..12329
                     /locus_tag="U8116_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN8433798.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            12332..12631
                     /locus_tag="U8116_000785"
     CDS             12332..12631
                     /locus_tag="U8116_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433799.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            12631..12945
                     /locus_tag="U8116_000786"
     CDS             12631..12945
                     /locus_tag="U8116_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN8433800.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            12938..13468
                     /locus_tag="U8116_000787"
     CDS             12938..13468
                     /locus_tag="U8116_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEN8433801.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            13465..13734
                     /locus_tag="U8116_000788"
     CDS             13465..13734
                     /locus_tag="U8116_000788"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433802.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            13664..13867
                     /locus_tag="U8116_000789"
     CDS             13664..13867
                     /locus_tag="U8116_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HEN8433803.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            13864..14151
                     /locus_tag="U8116_000790"
     CDS             13864..14151
                     /locus_tag="U8116_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433804.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            14638..15051
                     /locus_tag="U8116_000791"
     CDS             14638..15051
                     /locus_tag="U8116_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEN8433805.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15671..15835
                     /locus_tag="U8116_000792"
     CDS             15671..15835
                     /locus_tag="U8116_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433806.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            16247..16792
                     /locus_tag="U8116_000793"
     CDS             16247..16792
                     /locus_tag="U8116_000793"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8433807.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            16793..17110
                     /locus_tag="U8116_000794"
     CDS             16793..17110
                     /locus_tag="U8116_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN8433808.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(17267..17431)
                     /locus_tag="U8116_000795"
     CDS             complement(17267..17431)
                     /locus_tag="U8116_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEN8433809.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            17533..18054
                     /locus_tag="U8116_000796"
     CDS             17533..18054
                     /locus_tag="U8116_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEN8433810.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            18041..18514
                     /locus_tag="U8116_000797"
     CDS             18041..18514
                     /locus_tag="U8116_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEN8433811.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(18521..18679)
                     /locus_tag="U8116_000798"
     CDS             complement(18521..18679)
                     /locus_tag="U8116_000798"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEN8433812.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            18772..19533
                     /locus_tag="U8116_000799"
     CDS             18772..19533
                     /locus_tag="U8116_000799"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN8433813.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            19753..21072
                     /locus_tag="U8116_000800"
     CDS             19753..21072
                     /locus_tag="U8116_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN8433814.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            21088..22308
                     /locus_tag="U8116_000801"
     CDS             21088..22308
                     /locus_tag="U8116_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN8433815.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            22343..23020
                     /locus_tag="U8116_000802"
     CDS             22343..23020
                     /locus_tag="U8116_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN8433816.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            23017..24210
                     /locus_tag="U8116_000803"
     CDS             23017..24210
                     /locus_tag="U8116_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN8433817.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            24225..24551
                     /locus_tag="U8116_000804"
     CDS             24225..24551
                     /locus_tag="U8116_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN8433818.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            24555..24890
                     /locus_tag="U8116_000805"
     CDS             24555..24890
                     /locus_tag="U8116_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN8433819.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            24887..25282
                     /locus_tag="U8116_000806"
     CDS             24887..25282
                     /locus_tag="U8116_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433820.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            25279..25650
                     /locus_tag="U8116_000807"
     CDS             25279..25650
                     /locus_tag="U8116_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433821.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            25666..26226
                     /locus_tag="U8116_000808"
     CDS             25666..26226
                     /locus_tag="U8116_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN8433822.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            26351..26662
                     /locus_tag="U8116_000809"
     CDS             26351..26662
                     /locus_tag="U8116_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433823.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            26686..26877
                     /locus_tag="U8116_000810"
     CDS             26686..26877
                     /locus_tag="U8116_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433824.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            26877..30725
                     /locus_tag="U8116_000811"
     CDS             26877..30725
                     /locus_tag="U8116_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEN8433825.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            30726..31598
                     /locus_tag="U8116_000812"
     CDS             30726..31598
                     /locus_tag="U8116_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEN8433826.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            31595..32644
                     /locus_tag="U8116_000813"
     CDS             31595..32644
                     /locus_tag="U8116_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433827.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            32648..33472
                     /locus_tag="U8116_000814"
     CDS             32648..33472
                     /locus_tag="U8116_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433828.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            33490..35550
                     /locus_tag="U8116_000815"
     CDS             33490..35550
                     /locus_tag="U8116_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEN8433829.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            35567..35716
                     /locus_tag="U8116_000816"
     CDS             35567..35716
                     /locus_tag="U8116_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433830.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            35713..36159
                     /locus_tag="U8116_000817"
     CDS             35713..36159
                     /locus_tag="U8116_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433831.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            36161..36298
                     /locus_tag="U8116_000818"
     CDS             36161..36298
                     /locus_tag="U8116_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN8433832.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            36333..36575
                     /locus_tag="U8116_000819"
     CDS             36333..36575
                     /locus_tag="U8116_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HEN8433833.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            36591..36788
                     /locus_tag="U8116_000820"
     CDS             36591..36788
                     /locus_tag="U8116_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN8433834.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            36799..37815
                     /locus_tag="U8116_000821"
     CDS             36799..37815
                     /locus_tag="U8116_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN8433835.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            37930..39138
                     /locus_tag="U8116_000822"
     CDS             37930..39138
                     /locus_tag="U8116_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433836.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            39122..39292
                     /locus_tag="U8116_000823"
     CDS             39122..39292
                     /locus_tag="U8116_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433837.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT        13707 a         6600 c         8501 g        10737 t
ORIGIN      
        1 tatataacta accattcttc aacaagctca tgaaatgtta gatcaggctt atgaagtgtt
       61 ttttcgttta attttatatc aattttttta tttaactcta gcaatgccac tttctgagtt
      121 tctcgagatt tatttttata agtaatgctc actttttttc tttttctagt ttttggatca
      181 atatatcgct cattatattt atatactttt tttccgtttt tatcagtttt agtttcgatc
      241 cacattgttt tcatctccta tttgttgcta taataggaat agataagtaa gccgattata
      301 gcaggtttat ttttcatcac gtccacaaac tttggcgagg gaggggcgtg tttttgttag
      361 ttattgtggt aggttatcta aagcgtattg tgcttgctca tttgtaaaac cttcaaatat
      421 aagttgatcg tacaattgcg aatctgacat tgatgcccaa tcatcgtaac tttccgcttt
      481 agccaaagct tgttcgttgt aatcgacatc aacattagcc agcgcataat cgattgcatc
      541 acttggatat tcttcaaact ctagttgttc acgcaaacct tgttcagaca taccagccca
      601 acctagatag ctttcagctt tgctcaatgc atttctgtat tcacgaggaa cactttcttc
      661 ttgagtaact tcttctatag tagtttcagt agtttcagtt tcttcttcaa cgactgtaga
      721 ttctgctacc gattctgcag aagacacttc tgtcacgctt tctttagttg tttcagcgac
      781 ggtattttta gcatcatcac tgcctccttg cgaacctata gcaataaaca ccacaacaac
      841 cgctaacaac caaaaccata cccgtttata aaacggtttc ttaactttat acattttccc
      901 atcttgaccc ataacttttt ttgccattta aatatccctc gcttcttgtt ataatatatt
      961 tgtgatctca gaaatgaggt atgagtccgt gttgcagcac gggctttttt ctttataact
     1021 tttttagaga ttataggcaa aatagtaggg cataaaaatg tattattgaa ttccgtattt
     1081 agaaaatcct aattgaactt caccggaagt cttttgctgt gttgtacgca atgcttcttc
     1141 ttcagacatt ccattctgta ctttccatgc aacaggcgac atcccgtatt tgttaacaaa
     1201 atcagtaagt gataaagtgt cagcgtcttg ctgagcgctt gtttgttggt cttctggatt
     1261 ctgttgagat gctgcttgtt gttctttctg atcttgactg ataatattgc cagcatcatc
     1321 tgtagtcaat ccattttcat aaagggccac gccgaaagct tcccactctt tgttggacca
     1381 atttgcacga tcagctggag ttgactgtaa agtgcgttgt ttcatctgtt catatgtttc
     1441 ttcttgaggt gcggtttgga ttgtactctg actggagctt ataactgttg ggctaggttc
     1501 cgccgtagct tggttggagc ttgtaactgt tgaactagaa tctgttttag atgtagattt
     1561 gctagtagat gaactggttt cagttgtttc ttttgtttta cttatttttg tttcttggtt
     1621 agaagtggta tctgttgatt cagctttttt attatttgaa caagctgaaa gtagcagagc
     1681 agtacttaac aacaacataa cgctaacttt tttcatttta taattcctct ttctcgttgt
     1741 aatatgtgtg ctaacacggg cttttttata taagaaaacg ataagcgctt tctggaagcc
     1801 cgtaaagatt ctttaattcc tcgatttttt taggatattg atcattatct tctttataaa
     1861 gagaaacaat gagattagca gcaaagcaat tagcttcgct ttcagatttg cttctagatg
     1921 ttcttgttga tacataatag ctggataagc cacgatgaaa aatagcgtga cctaattcgt
     1981 gagcgcaaat gtagaatctt tcctcagagt ctcgcagttc atcatttaaa aagattatcg
     2041 cacgatctct aatttcttga aactgcccct tgggattctc gataaaagga acgtattgaa
     2101 ttttaatgcc catcttttca caaatataaa aaggattagc ggactggtat ttccgcttca
     2161 actcctcgac taaattaatc gtatccatct ccataagctc acatcttttt gcctttttct
     2221 ttgtcttctt tcacaatatc ccagaaagtc gctatcagga tatcttttac gcgctgtatt
     2281 tgttcgggtg tcaatgtttc cccaccataa gacatattaa catttgagtc tagtagttta
     2341 tcaagttcaa ccacttcctc ttttgtagcc cacttgggaa cattattatt tcccaataaa
     2401 taatcagttg tgacaccaaa ataatcagca actttcttta agttctcaga ttttggcgag
     2461 gctttatccc atcttcttat ttgtccatta gagatgccca cctgtctttc tacttctgct
     2521 atagtcacat gcttttcgtc tgctaattct ttaatcttag taactaaact cattattatc
     2581 aacctttcaa agctgaaaag aaaataaata gcttaaaagt tatatttttg gttgacaatt
     2641 agcttttaag ctagtatatt taattcgtaa gctaaattgt tagctaaata agagcaacaa
     2701 aaaactctac taatttaaaa cattctctcg gtcgccaaac ttagaaatgt tattttagag
     2761 gctttttata agtcttattt aactatgtat tcataatagc ttaaaagcta ataggtgtca
     2821 acgatttagc taatttttta gcttacaaat tatttgttta gaaaggagct atttttatgt
     2881 ctgagaattt agacttaaaa attcgagcgg agatgagaaa aagaagaatg actttcaaag
     2941 aactagctgc gcttatcggt atttcaggag cttatttatc agatattcta aacggcaatc
     3001 gtgatggaaa gaaagcacaa cagcatatcg aaacagtgaa aaaaatattg gacatccgat
     3061 agggggggta ggcgatgaca aaactaaaaa aacaagattt tgtaaaaaaa tataattatt
     3121 ctccatctac ttatcaacgt cgaatgtcgg aactaaaaaa tacagcaatt ttctcagcgg
     3181 cgtatgaacg ggtcacagga caagaagttt ggatcaatac agaattatac gataaatttt
     3241 tgtctttcaa atcctataac aggttacgca caagaaaggt aacacctaaa gaatttatcg
     3301 agaagcattt agttgattta taaaaaacag aacattttga gaggtgaagg ttaatgggta
     3361 aattcaacag agcattagta ttcagcgcac cgctaatcgt ctatgcttta gggctttggg
     3421 gaagtagaca agcgttgata ggaacgattg tgtacatggt ttggattttt atagggctgg
     3481 atgaagctga gtacaaaaca aaaaagccag tcgggaggga ctgactaatg agtaaggacg
     3541 atattgtatt tctgttaatt atgttttgtg ggattggatc aataataatt aatttgtatt
     3601 tgattattaa agaaaaattt ttcaattaaa atgagtcttt tagctttaac ttaaattttt
     3661 tttgataatt agctgttctt atgaccatta caagtttttc accaggtaat atatctttca
     3721 tatctaaaac ttctgaaaaa gcgactgagt agccaggaga gattgcttgt gccaacggta
     3781 taggagataa aaggctactt ttatgaatgc ttcccatatc aaaaccattg gcgtgataat
     3841 gtgaacaatt aattttctta ccgttgtaaa gatacgtaca ttcgataatt gagtttggca
     3901 tttttgatgg gtttgcaatt acgccagtaa gaatcattcg attacttcta tcatgcttaa
     3961 ctaaaaaata ggagagcgta atttttcttt tatctttaaa taagttatag agggataaaa
     4021 aaaatcctga aatagaaatg attaaagtaa tagcgacttg gtaatcgctc aaaattttta
     4081 tcaagtaatt caccaccttt ttactcaaat tatatcaaaa aggagagaag aaataatgca
     4141 agaattagta attttgaaaa ataaaaaaag acacgaacgc cggcaagcat gatcgtgtca
     4201 gggaaataaa ctatctttct atattttacc acagaaagga atgaaccgta aatgctaatt
     4261 gcaacggata cactagaccg catcttttta aaagacgaat acaaactgcg caatatagat
     4321 gcgtcaggaa ttttagtatt tgatctttat gacaatggaa aaattggtat ctatcaagca
     4381 agtgatattg aagaaacaaa ccttgctttc gaacaaattg atgattctgt ggaattggat
     4441 ttagatgagg caatcctagc ttttgaacaa attgcaaaat tattaaagga ggcacaaaag
     4501 aatggcaact ctttaccaac tcagcgagtc atatatcaaa gtcctggaac tggcagaaca
     4561 attggatgaa gaaattcttc gcgatactct cgattcgatt aatgaagcga tcgagtataa
     4621 ggcagaaaac ttagcaaaaa tagttaaaga agtagaaggg aaagctgagt taatcgatag
     4681 tgaaattaaa cgtctgcagg aacgtaagac atcgcttttg aacaatgcta agagtatcaa
     4741 gcactattta caagaggaaa tggaaaagac tggtaaaacg aagattaaag gtgaattatt
     4801 caacattgga attcaaaata atccagtatc ggtcaatgta atcaatgaga acttaattcc
     4861 aaaaggattc tttacccctg tgcctcccaa attggataaa aagcaattga aggaggaact
     4921 gaagcacgga gatattcctg gtgctgaact cgtccaaaca aaaggtttga ggataagatg
     4981 atgaaggagg agaaataatg gaacacgaaa acgtaaaaga cgctttgaaa gctgctatcg
     5041 aaatcgcgga agctaaagga ataaaggttg acggcaaacc agcaactgtc catgacattc
     5101 aaaatttgac gaaagaacat ctttatttta tcgccgactt gcttggtttg tcggaattgt
     5161 atttagacaa ataaccccac acggaaggag tggctagggt ggttgtatta caagtaatag
     5221 tctgcgtaac attcatttgg tcgtgcatac cggtttgtat atatatcatg aagaagcttc
     5281 gccctgagtc ttacaaatta tacgcaattt acagcataac agtagctctt gcatttacat
     5341 tgtttgcaat ttctaggctt ccgttttaat cgtgggaatg aatttttaat gaactaaatt
     5401 atctataaag caaaaaaagg agaaacaaca ttgaaaaaca aactagcaaa aacaacagca
     5461 atcatcggac tagcactagg tagcggcgtt atcggctacg cagcaagcaa tgcgtttcaa
     5521 gatttggaca cgatcaaagc aaatttcaac acagtcctac aatacggtca aacgaaatcg
     5581 caacgtgtgt cagaacttga atcacagcta tccaacaaca ctcgcacaca ggagcagctg
     5641 aaagccgaaa ttgagcaaat caaatcggac aagcagaagg aaattgaagc caagcaacga
     5701 gaagtcgaac aaaagcaaca ggagatcgct acaaagcaac aggaagccga tagcttgcgc
     5761 caacaactat ccacagtgca aaacgacaag gagcagttag aacagcgcgt gagcgagtta
     5821 cggcagtata cggatcaaaa ggtaggggag ttggggaaat gaaagagttg actatttatg
     5881 acccaaatat ttcatgggct aaacacacaa ttaaagtttc ttttatgatt tggggttaca
     5941 aaggatatgt tacttacaaa gtgggcggta acactaaggg tttatcatta atagctattg
     6001 attcagatga tctatatgat gctaatttcg aggataaccc agttaatttc cgtgaccttg
     6061 atgaagattg gttctctatg gaacttacta acgataaagg cgacagtaca cttgtagaag
     6121 atgaattcga cagactaggt gattatattg tcggtgttga aattattgcg cacgaaccag
     6181 agtaggagag tgaatacaaa atgattaaaa gaatcactat atcagttgaa gaagatagtg
     6241 gatcaacaat gcagcatacc gacaatcgaa aaactttttt aaccaataga aaggagaatt
     6301 ttgatgcaga ttaagaaggc gacggatatc gaacaaacca agggaacata tttgatctac
     6361 ggtgctccag gaaaaggtaa aacttcaacg gctaaatatt ttccagggaa aacactgatc
     6421 cttgatattg atcggacttc aaaagtcctt agaggtgaga aaaatgtcga tatcgtatat
     6481 atcgacaacg aagatacctg gaacgactgg ggcaacacgt tggctgattt aactaccaat
     6541 tatgtaggtg tttatgacaa cgtggttgtc gataatgtca gcgaattgga acggtgtatt
     6601 ctgtccagtt taggcgcaga aggaaaaaac aacggcgttc cttcgcaagg cgattatcaa
     6661 tatatgcaat ttagaatggt gaacagccta cgctatatga aaaatttaga cagcaacctt
     6721 atctggaccg catgggaaga aatcgatttg tggacggatt cggatggatc ttcttacaac
     6781 atcgctttgc cgcagatcaa tcgaaagatt cgaaacaata ttttgggatt atgtgacgtt
     6841 gttggtcgat tgatggtaaa ggaagacgat gaacgcggat ttattttaca agccacagat
     6901 tcaacctatg ccaaaaatca attggataat agatctggct gtaagcaaag tgagctgatt
     6961 ttagatggaa ttgtacgact accagaatga tttagtcgat cgtgcaagac aagcctatgt
     7021 tgatggttac aaggcaccat gtatcgtatc accgtgcggt tctggaaaat cagtcatggt
     7081 tgcagagatc gctaaacgaa ccacacgaaa aggcaacaga gtgttgttct tggtccatcg
     7141 tcgtgaattg attgatcaga tcaaagatac gtttcaaaag atgggcgtag ataatcagct
     7201 tgtaaatttc ggcatggttc aaacagtcgt acgacatctt aaaaccatca agaaaccaca
     7261 actgatcatc actgacgaaa accatcacgg gttagcagcg tcctacagaa aaatctatga
     7321 gtattattcg gacgttccgc gattaggttt taccgcgaca cccatccggt taaacggtag
     7381 tgggttgggg gatgtgaacg acttgctaat tgaaggagta tcggctaaat ggttgattga
     7441 gaatcataga ttagctcctt atgaatatta tgcacctaaa ttgatcgata cagccgaatt
     7501 aaagaaagct tcgactggag attttacaaa gaaatcaatg gataaggcag tgaagaatac
     7561 tatttatggc gacgtgttga aacattataa aacgttagca gaaggtgaac aggcaatagc
     7621 atactgtcac agtatcgatg caagtaaaca cactgctgaa atattcaacg atgctggtta
     7681 taaagcaaca catatcgatg caaaaactcc aaaagatgaa cgtgcagata ttatcgaagc
     7741 gttcagaaat catgaaatta aaatcctatg caatgttgat ttgatcggag aagggtttga
     7801 tgttcctgat tgttcgactg taattatgtt gagacctact caatcgttat cattgtacat
     7861 tcagcaatcg atgaggggga tgcgttaccg accagataaa acgtcaatca tcattgacca
     7921 tgtgggtaat gtgaaccgtt ttggattgcc tgatatggat cgtgaatgga gtttagatac
     7981 aaaaaagaaa acaaaaactg atagtgattt atctatcgtt caatgcgcgt tttgttttgg
     8041 tgcttacgaa cggccaaaag gcgataatat ttgtccgtat tgcggagagt tgcagccgat
     8101 tgaagaacga aagtcagagt tagaaatcga tgaatcggct gaattaatga aagtcggaga
     8161 aacgaaaatt actttgaatt ttgagaacaa taaatattac aacatgacgg aagatgaagc
     8221 agaaagtata gaggacttat atgccatagc aagagctaaa ggatttaaac ctggttgggc
     8281 ttatatggct gctaagagga aggggtggtt gtaggaatgg gcggtatttt tggtaaaaaa
     8341 gcagaagatg ttcaaggttc aataataaat ggcgtaacaa taataggtga tactggaaaa
     8401 agagatagtt caggcaatca aattgtaatc gctagaaata aaaataacga agtttttgaa
     8461 tcttctctat ctaatataaa aagaggtgtt gttaacggga caggcgaaga gagtggctac
     8521 gttaatttaa aaaaagctaa tgaaaaatac catgttgaag gaacttacgt taaggctttg
     8581 tctgtcggtg gaacaaaagg cgttaattgg gataaacgaa gaaataaatg gaaagcacga
     8641 ttatatttca aaggagaata tgtcttacaa aaggatttca acaccaaaga agaagcaatc
     8701 caagcacgac ttgcagcaga agaaaaatat ttcaaaccta tcttagaaaa atatgaaaat
     8761 aaaaaggaga atgattaaca tgacaggatt taacttagac ttttcaaaag cgcaacaagg
     8821 gaacgaaatc aaagacggta cgtacgaagt ggtcgtaaac aaagctgtag aaaatgcaac
     8881 taaatcaggc gcagaattta tcgatatcga tttgatcgtt cgtaacgatg tggatcaacc
     8941 gttccaaaat aaacacattt tcgcaaaaat ctggaaagcc aaagctaccg gcaaatataa
     9001 cgaaggtatg atcatggcaa ttgcacaagc gctacagttg gaagacggaa aatcatataa
     9061 cgggtttgat gaattgctgg cggactttgt tttgaaaacc gcctcagtac gcgtgaaaac
     9121 tgaagagtca aatggatata aaaacgtcaa tgtgaagtct tgggataaaa cgaacacacg
     9181 gggcgtaatg aatcaccaat tcaaaaatgg tgatgaacca tccttcggac cggaacgttc
     9241 aagagcgaca acggttagaa atgacaatct tccattttaa ttgaggtgta atcaatgtat
     9301 gaaaaaattc caaaagagct taaaaatctc aaacaatggt gtgtttataa gctagtttgg
     9361 gatgaaaagc ggcagaagca cacgaagatc ccttataacg ccaacaacgg gcataaagcc
     9421 aaatccaatg acgagagtac ctggtctgat tttcagaccg ctctcgctgc tattgatcac
     9481 tacggaatga gtggactggg gttctttttc aaaccacctt atttcggcat tgatatcgat
     9541 aatgctgaag gtgaagttga gcgatttaaa tcaggtgata tagaagaaaa tattatctat
     9601 gaatttatcg aaagcatgag atcctatgct gagtattctc aatcaggtac tggcatccac
     9661 attattgctc gcggagaatt gcccggcgga agacgcagga aaggtgacgt agaaatgtac
     9721 cagaacggcc gattcttcgt tatgactgga aatgccgcat caaaatattt agaaataact
     9781 gagccgaatc caaaggatat caaacgccta tatgatcgct acgttggaga tgaaaaaatc
     9841 atccaattca aagaagaaaa tccgctgatg aataccgttg atttaccggt tgaagaaatt
     9901 atcaaacgtg ctgaatcgtc ttcccaaggg gcaagattca aagtctttat gaacggcggt
     9961 tgggaagctt tttattcctc gcaatccgaa gcggatatgg cgttcgccaa cgacttggct
    10021 ttttggaccg gtagagactt tgaaaaaatg gatgagattt tcagagcgtc tgcaatgatc
    10081 cgtagcaaat atgaccaaaa aaggggatcg gcaacttacg gagaatctct actaaataag
    10141 gcaattgctg atacaaattc cgtctacaac cccaaacgga aatctgattt taagattttt
    10201 atcaaagacc aggaacagcc aaaagaggaa aaatactatt cgtacgacga caccggcaac
    10261 gcggatcggt ttactgatat ctacggaacg ttagtcaaat attcgtacat tgataaatct
    10321 tggtattact acgatgggaa agtttggcta caggacaata ccggcgaagt tcggaaaatg
    10381 atcgatacga cagtagacat catgggaaaa gaaccgttaa cgatccctga aggcgcagac
    10441 gatgaaacca aagaagcttt gatgaaagcg aaagaaaaac acgtcaagcg ttctcgcagc
    10501 aatgccggaa aaaacgctat gatggacgaa ttgaaacatc gactatccgt attgccggaa
    10561 gagttcgaca aagataaaac gctgttcaac acacaaagcg gctatctgtc tttgcatgac
    10621 ggtcttctac acgagcatga gatagacaaa atgtttacac gtgtttcaaa tgtcgagtac
    10681 acagaatcgg tcgattgtcc tatgtgggaa gaatttatta atcagatttt tgacaatgat
    10741 caagaattaa tcagatatat ccagaaatgt gtcggatatt ccctgaccgg atctaccaga
    10801 gagcaatgta tgttcatcct ctacggtcat gggtcaaatg gaaagtcggt atttttggaa
    10861 attatttctg aattgatggg aaactatgcg atgactatgc aggcacaaac gatcatggtc
    10921 aagcaaagcc aatcatcagc aaacagtgat attgcacgat tgaaaggtgc gcggttggtc
    10981 acttcgtcag aaccgaatga aggcgtccga ctggacgaag gtctggtcaa acaattgact
    11041 ggtggtgata aagtcacggc tcggcacctt tatggaaaag aattcgagtt cgaacctgaa
    11101 ttcaagttgt ggctggcaac gaaccacaag cccatcatcc gtgggaccga tgacggcatc
    11161 tggcgacggt taaatttaat tccattcact gtccaaatcc cggatcacaa aaaggataaa
    11221 aatctgaaat ttaaattgca gacggagttg caagggatcc ttaaatgggc aatcgatggt
    11281 tgcctgttgt ggcagagaga gggcttggag aaaccaaagt cggttgttgc agcgagtcag
    11341 gattatcgaa acgaaatgga tcaaataggt acatttatcg aaacgtgttg cgaaacaggt
    11401 ccaggactaa aaatatcagg cggagaatta tacaaagttt atcgtgaatg ggcatcagat
    11461 aatggcgagc atactttcac taacacgaag tttggcagag agatttcaaa gaaatatagc
    11521 aaagaaaaag ctggtggatt tatggtttat aaaggaatca cattaaaacc aagaaaatac
    11581 gataacgtta gagaactttt taaataaatc tggagggttc tggagggttg agaacgaaac
    11641 cctccaaccc ttagaaacgt tgatatatca gtatttatat tatatttttc tttctttctg
    11701 gagggtttta aaaaaataat aaagtattaa agtaaaaaat aaaaagtaaa ttatataaaa
    11761 aagaataggt ttttgggtct gaccctccaa actctccacc aaaatcttcc taaatgcttt
    11821 gataccaacg attaaacagt ctggagggtt acattgaaat cagaacatca aatacaagac
    11881 gagatcagaa ttgccttatc aaaacatggt tgcacgattt ttcgatccaa tgcgggacaa
    11941 gtgacaacga tcgaagggcg gcaattttac gggatgccaa aagggttccc agatctatgt
    12001 ggacatcgta attctgatgg gaaagcaatt tatatagaag taaaaaatga acgcggtaag
    12061 ttgcgtgaag atcaaaaaag gtttgctgaa tttttaaacg gtcaacctgt actatacggt
    12121 gttgcacgat cagtggaaga agcattgaaa attgtggagg aatcatcatg acaacagaag
    12181 aagtgattca aatgcgtatt cgaagccttc agcgtgagat tgacgatctg gaacggacaa
    12241 aggcagtgat ggtcaatgaa acggcgagga aggcaatcga tttgcacata gagaatttaa
    12301 gaagggaaat ccatcgattg gaggaatgag cgtggataag aaagcagcaa tgaaacgaat
    12361 catcgaactg acacattctg agaattggca agaagacaaa gaaatagttg cagaagtcca
    12421 aaagctcggt aaatcaatgt ggagtgaaaa aaccaaacgg agaacaccga gaaaaattgc
    12481 aatctggcat ggtgaccgaa ttctagtaac aggtactgct gaacagttat ctgaaattac
    12541 tggattaagc aaaaacatta tctgggatag agctaaacgc gagaacgttg attcgaaagg
    12601 tcgtcaattt aaacattggg agaagaaata atggaggaac taatcacaaa agtagagcag
    12661 tgggcaaaag ataagggatt ggatcaagct gattccagca agcaaatgtt aaaaacgatc
    12721 gaagagattg gggaagttgc cgcttctcta gctagaaaag atgaatatgg tttaagagac
    12781 ggaattggag acgtagtagt aaccttgatt attttagcta tgcaaaatga tatggatttg
    12841 tacgagtgtc tgaaccaagc atacaacgaa atcaaaggac gcacaggaaa aatggtagat
    12901 ggtgtattcg tgaagtcgag tgatttggag gacagcgatg aataaaaaag tattaattga
    12961 taaacaagca ttgattgatg aattaatgaa aatacctggt gtgggatcta atagtgacgc
    13021 tttagaaacg attaaacgtt tcccatcgta tgaaccgcag aagccagttg tgcctaagtt
    13081 tgtggcggaa ctactcgact attatcgtca gtcaacagat gttgatttat tagctttatt
    13141 gataactttc catgattggt attatcgcaa aactaaagac ggtgagcatg aagaagcaat
    13201 tgattggcta gttgatcatc ctgaaattta tatgcgtgca tggctgtttg gctacgagct
    13261 cgagaaagag ccgttgtatt gcgtaataat caatggtaga tatcttgtca aagtattcag
    13321 caatacaaac gttgtcatat tagttccagc agatgaattt tcacagtacg ttactcaaaa
    13381 atttcaatta actgaaaaac aaatcaaagc aattgatgaa agatactggc cgtttgctgt
    13441 gccagtggat gaggtggtag aaggatgagc gacccataca ttactaaatt agaaaagttg
    13501 tccgaagatt ttgagaagaa taaaaataaa ctaactaaag ataaacttat agaactggtt
    13561 aggttggtta tcagaggagc tattacaaga gaaaaggcgc agcaaaacat agaaattttt
    13621 gctcgtagtg tccgagatgg attagagaag aaagttaaac aaaatgaaca acagacaccg
    13681 cagaataaca aaactaagaa aacgggaact gaatgtacta aagacaaagt ttgaaaaaga
    13741 atatggaatt tcagcagaag aaacatataa agtggcaagt cagtgtgttg ctgatgcgag
    13801 cggcgctatt cgtaagtttg gaatttcgat attaagtgat gatcgtaaat gggaggaaaa
    13861 agaatgaaac taaaagacgg attttactcc agcagtcatg gtatcggcgg tttaatgcta
    13921 gatatgccga caaagaaccc taaaacacgt gagaaaccaa aattcaaagt cggtgacatg
    13981 gttcgctgtg aagcagaagg attcatctat ccattttgtg gatatgtaga acatctctat
    14041 aatcactcag caatcattcg tattgaaaac acgatggaat gtgacaagtg gttagcgaaa
    14101 agcaaagaga atttagcagt agcgagattg gtggatatgg aggttatata aacagcgaaa
    14161 aaatgtactt ctaagagaaa aatttatttt ttataaaaat aggaaagtat cattatgaaa
    14221 aagaccacag agataaataa acctaccaca taaaaaatga atatttcttg cgattcaata
    14281 taggtatatt taacaatttg tacaataaaa aatactatag gtaaaaaaga taacatgagg
    14341 agcttatttg ttctagagtg tttgacaaag taacaaaatg cgacgatacc aatacaaacg
    14401 ggaatccaaa ataacagcat gctccacata gtcactcaac catctcctat acgctttatt
    14461 atcaatagat aatatcattt ttcgtaataa agataaaaaa ataagtaagg cttctcatta
    14521 aaatataaaa aagacagccg accactgact gcctatataa gagtattgaa ataaaaagct
    14581 gctgatataa taaattccac aagtttatta tatcacataa aggagcggtt tgacttgatg
    14641 caattgttac gagaggtaga tttcaaacag acaagatgta atgcgagaga tgtgctgaag
    14701 aactttcggc gtttggagcg gatggcaggt cgctctttga tagatattaa gtcgccgatt
    14761 attacggata tgccgaaggc gccgaagcac ggcaataagg cagaagacgc aatcattcag
    14821 atgatggata tagaagcgga gagagacgcg attctagcag ccttgatggc tcttagtctg
    14881 attagtcgtc agatactcta ctatagcttt tgtgtgccag atagcttctc aaactacaga
    14941 attagccgtg aagtgggtta ttcagaaaga agtatacaac ggatgaagtc ggaagctcta
    15001 atagagtttg cagaagcata taaacacgga agaataattg cttataaata atttggcggc
    15061 tttttggcgg aatgatggcg gtttttagct atttaccagt gatattatgg tagtgtcgaa
    15121 agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt attattgttt
    15181 cacaattaag cttcgataga cagcaacgga aatattaaga ataaggatgt gaattttaac
    15241 tccttctaaa ttgttcttat tatctatcat ccgttgctgt ctattgtttt tttaattatt
    15301 cacacgataa ctaaaggtga gcgaagagaa atgattccat taataatttc aatttttgcg
    15361 ctttgtctta atgtctatat gattggattt aaaaatgggc aaaataaaag atagtagtaa
    15421 ctaagaaaga aatttttata ttgtcactgt ggcggaaagg gtagacgcta agcatgtgtg
    15481 ctaggtcaat gcttcggcaa ctatgcaatg ttcgattcat tgccagtgac tttggcaacc
    15541 gaggcatcgg cggtttaaaa atataggggt gcgcaatttc gtacgcgttt tgtgcatcgt
    15601 gtaggttgct attacatatt agatcactcg ttgagtggtc tttttatttt acataaagga
    15661 ggtaacaacg atgtatagac cacaatactt agaacagaag tatgaagtaa tcactgtgca
    15721 aaacggtaac ggtgagatag tacgaaagta tagaagacca ataaagagcg atacatataa
    15781 acgaaaggaa aacaatgaag ttatttcttt tcgcagaagg agaaaagcca aatgagaaac
    15841 tactggtatg tatcgctaac taatgaatat cctcgaacca ttgatgattg ttcagtgcgt
    15901 gttgtgcgtt ctgtacaaat caaagggaag tactctattg tcgaaatgct aagagaagct
    15961 acaccaaacg aagtggataa atgcaagctg atatattgcg gtcatggcta ttggaaagac
    16021 gagtatatcc aacaaaacat tgagaggtgg atagatagat gagttacctc gaacatttga
    16081 aacgttgcta catgcattct aagaataaac ttcctgacag ctacactgta gatgatgttg
    16141 ctatacatgt attgaagact gagagccata gcagtcctga tggtagcagt aaggagcaga
    16201 cgctcgcgtg gttcaagttc tttaagtgga ttaaggagga agagtaatgc acatgcacat
    16261 aggagaaaag atatataaat tgagacggct taataacgaa acaatgaacg acttagttaa
    16321 acaaactgga gtgtcaaaat catggttatc tgatattgag tctggaaaaa agcaacgcgt
    16381 tgattttaat aagatatgtg caatagccaa acattataaa gttagcttag aatactttcg
    16441 ggatgattat ttggatagtg atgaacacga ggatattatt cctttgacag gcgaaacaag
    16501 agttaaatca gtgaaatcgc ttgaacaaag cctaaagtat ctgaataaca aacaagaacc
    16561 taaccttcta gagatcaaac taaaagatac tgactcagta ccagaggttt ggtacaaggg
    16621 tgagaggttg gatgaattgc ctaaaggatt agtagatgtc tcgtatcatt ggaagactga
    16681 tgattttact aatgatgata gaggagcgaa cgacatcacg attcaatact tttctagctt
    16741 taatgataag tatccagata agaaaacaat cggacacaag agagatatgt aaatgaaaga
    16801 agttagacct agagacgaaa tagacaaact atacaagacc aaacgatggc gagacctaag
    16861 gcaagtagta atagctaggg acttcggcat gtgccaagag tgcaagcgtc gagggcggaa
    16921 cacaaggggc acgatcatcc atcacatagt cgaggcgagg gaagacctgt cactgttctg
    16981 gtccgtagat aaccttgaat gtatctgtgt agcttgtcac aacagagagc atccagagag
    17041 atcaggcggg aagaagaaac caaaacctaa atcacatatc gttaaaatgt attcaactcc
    17101 tgaaagataa gtttgcagcg aaatgaaggt agccccccta ctctaaaaga ttaaagagta
    17161 aggcttgaga agaacggtgc tgtccttcct tcgtaaaaag accgcttttc aagttttttg
    17221 gagaaaaagg aaaaagccga ccaatttaag ccggctttgg acgaagctat ttcttagtcc
    17281 atttgtttcc tttttgagaa gtagggggta atcggtcgcc tggatcaatt tttacttctt
    17341 gtccgccttg gacatttcca ccacgaggac ccacttcttt gtaggttcct tttggtttgt
    17401 tgtcttcgcc gggtttatag agttctccca taggaatccc tccttaacaa atttcggcac
    17461 agcactgccg ataacctaat tataaggatt gtgataacga tttcaatcta tcttttgaaa
    17521 gaaggtgata ttatgccgca accagcgaag agtgcaaaat tacaattatt aaacggaaac
    17581 ccaaataaga agaataccga agaactccgc aagcgagcgg ccgcagaaga caaattaaaa
    17641 atggctactg acaaaatcaa accgccgtta tggctagatt cgctaggaaa ggataccttt
    17701 gagtttatcg ccgatgaatt gctgtctgtg gatttaatca gtaatccgga cgtccataca
    17761 atggctctat actccaattg gtattcgcaa tacgtttctt tagaaaaaca gcttcgaaaa
    17821 ctacaacgag agtacaagtt gaactatgcg cttgcgaaaa aggaggcaga ggcgagaggt
    17881 gagccgttta atgaacctaa tgaattaatt ggtaatccgc tctctcggca gatggataca
    17941 gcgtctcgga atctccgttc ttttggcgct gatttaggac tatcaccagc agccagagct
    18001 aagttagcta ttaagatggc tgatgatggt ggtgatgacg atgacgactt ctaatatttt
    18061 ggatatgtct tacacagaac gtgtggacta ttggcaaagc tatcttgagg agcaagcttc
    18121 ttggggtggc tttttaaaat gtccatatcc ggaattgtta actacttggt atgcggaacg
    18181 attaatcgat ggaagcatac cagccagcaa agaaaatatt caagctgcta aacggcatat
    18241 gcgtgatttg cagcgccaag gaacagatga ttttccttgg atctttgacg aagaaaaagg
    18301 tcaccggcct attagatata tcgaaaaaaa atgtaaacca actgaaggcg actttggttc
    18361 gtttgttttg caaccttggc agcatttcat aattggatcc atgtacggat gggtacatcg
    18421 tgatacagga gagcgtcgct tccgcgaggc tcttattttt gttggacgta aaaacggtaa
    18481 gttttgccgt ttaaaaatcg ggcaaaatcg gtgaaaacct ttatttttgg ctttctttta
    18541 gtaataattc gatagcttta tcaagcaatt tagacatagg aaccatagtt tcttccgaca
    18601 ttttttttaa tccttcgtat aaatctttat caattgcgtt agaaattcta attctgtttt
    18661 ttagtccgta tttgtccatg tgtgacactc ctttttattt tactataaca tccgattcca
    18721 ccgcttgcaa ctaccatcaa ttgatggtag tatataaaag aggtgagtag attgaaaaaa
    18781 tacattattt acaaacactt aaataaaata aatggcaaaa tttatatcgg tgtcacaaat
    18841 gatattggca gaaggtggag gagcggtggg attgaatata aaccaccaaa gaacgaaacc
    18901 caacaccaca gaagtttttg gaatgcaata caaaaatacg ggtgggaaaa ttttgatcat
    18961 ttgattattg aagaagactt aacgatgaaa gaagcttttg agaaagaaaa gttttatatt
    19021 gaactttatg actcaactaa taagaaaaaa ggttataaca tcgcaaaagg cggaaatggc
    19081 ggaataatat ataaagttca ccccaaagga atgctaggga aaaaacaatc taaagaattt
    19141 tcttcaaatc acagtaaatg ggcaaagaac cataagaata attgtatgac aaacggtgat
    19201 gttgtatggg gtgttactca cgaacaccca aaaggcatgt tgggaaagca ccacagcaaa
    19261 gaatcaatta tgaaaaagaa agcatatagt ggtgaaaatg ctgtaacctc aaaaccaatt
    19321 gtcgcaattg aacgggatgg aactaaaaga gaatttcaca gcgctaagct ttgcatggct
    19381 tactattcga ttagtacatg tgttttttac cgacttttaa aagatggcgc cccttatgtg
    19441 ataaatccta aagcgaacta cagaaataaa gaaaaaatat tagctatcga aggaattatg
    19501 ttcaaacata aagaagatac cgaggtaagt tagtagattg cgaaaggtta ctaactaccg
    19561 taacgcgtag gagttgaata aatataatgc tcccaagagt gtccgacagc agataatttg
    19621 ctgaaaatgt acgctaaact gggccagaac tgactgaccg atgaaaatga gggtgacctc
    19681 cagagtgcga gataaaaagc tcgcagataa taacaatcga aaacatcact aatttcaggc
    19741 ctttccacat acatggtcgc ttatgatgat gaacaaggcg ccaacgttta cgtattggca
    19801 aatgctcgtg atcaagcaag cttgttgttt gataaggccg cagaaatggt caaacaatcg
    19861 ccggcgctct ttaagaaatt tggtaagcct aaacgatcaa gtattaatta tgctcccgcc
    19921 ttttctaaaa tggaaccacg cgcctcagat agccggaaat tggatgggct aaacactcac
    19981 tttggtattt ttgacgagat ccacgagttt acgaattaca atctgatcaa cgttatcaag
    20041 aaatcaagag gaaccagaaa acagcctctg atagtttata tcacaactgc tggatatgta
    20101 ttagatggtc cgttgatgtc ttattttgag caaggtgtgg actgtttgga acatttggaa
    20161 gatgacatcg atgaacggac tttctattat ctggcaaaac ttgacagtgc ggaagaggct
    20221 gatgacccaa gattatggat caaagccaat ccgaatattt gtctaatgaa ttttgttggc
    20281 atgctagatg actatgttaa ggataaaaaa gatccaaaag aatatgctga ctggattacc
    20341 aagcaattta acttgttttc cgatatcgat gagctgtcat ttgtcgatat gcctaccatt
    20401 aaacgaaaca ataaaaccat cgatattgaa acgctcaaag gtaagaagtg tgtcggtggt
    20461 tttgacttgt ccgaaacgga agactttacc gcagccgttt tagaatttcc gcttgaaaca
    20521 ggcgaggtat tcattttgca acacacatgg atcccacaag ctagatttga tcgagataac
    20581 aatcaagagc gtatcaaagc gtgggagaag gtgggagatc taacgattat tcctggtgat
    20641 tacgtcaatt atgaatacgt cttaaattgg tttgtagaaa attcgaaaat ctatgacatt
    20701 gtaaaaatca attatgacaa ggccaaggcg ctacgattaa ataaagaact agaaaatgca
    20761 ggatttgaaa ccaacgagat tcggcaaggg tttctatcat taggtgggcc aatgcaaaac
    20821 ttcaaggaaa tgctattgga cggtaaggtg attttcaaca attccaagct ttaccgatgg
    20881 tatctatcca acgtcaagct ggtgatggat cgcaactcaa actggatgcc gtctaagcag
    20941 tccaagagta gaaaaataga tggttttgca gcaagtttga acagccacgc cgaagtgttg
    21001 aatatgttgg ttaatcctgt cggaaccggg aaagtaacct attactcgat ttccgattta
    21061 atgaatatgt aagaaaggtg tggaggaatg agtattttag atcgtttgcg ttcttttggc
    21121 cgagcgaagc cgaaagcgag caaacaagag tattttttga atgacccggg attgataccg
    21181 tatttagtcg gaaaagatga aatatcagaa gggatttttt ccgtaattag ccgtgtatcg
    21241 aacgtttttg cgtctctccc tctcaaaatg atagatgtgg agtttggcca accggacgac
    21301 tgtcctgcat acaacttgtt gagcgaaggc cctcgatatt ttacaaagtt tgattttttc
    21361 cgggacgtgg aagttttgag aaactaccaa gggaatgcgt atgtgcagat tttccgaaat
    21421 atcaatggag aagtagcaga tatggcgtta gtaaaacctg gtgcttgcca tccagtgatt
    21481 gatatggata gcggggagct ttactaccaa gtaactgcga ctgacaaagg cagttacaag
    21541 caagttatct atgtacatta catggaaatg cttcacttta aacaaccgag gtttggcggc
    21601 ttggaaggta cagaccccac aaaagtatta acgaataccc tcggatatga tcgagaagtc
    21661 cgaaaaatct ctttaagtca gcttaaagga agtaatgaag ggctaaaagt taagtttgct
    21721 agcaatatgg atgaagaagc taaaaaagct acagttaaaa acattgctga tttttatcga
    21781 caaaacggtg gactacttgt ggaagaaaac ggtgtagaaa tcgaacgttt acaacgagag
    21841 ctggtagaca gcaagctttt agatactgat aaagtatctc gctccagaat cgctatggtc
    21901 tacaatgtgc cggagcattt catcgggaat aaccagtcga gttactcctc tcaggaacag
    21961 ctcaatatgg aatttttgac gtacaatcta gtaccgaccg ttaatcaata tgaagcggaa
    22021 ctaaataaga aaacactatc gagaaccgaa aaagcaaaag gttatcgata taagtttaat
    22081 gtcgcaagtt tgctaagagc tgacactcag gcaaggggac aattctatca aatcatgcga
    22141 cgaggcggag catactcagc gaatgatgtc cgaagatttg aggacttgca accgataaat
    22201 aaaaccggta tggatgatta ccatatttcc ggagacctat atccaatcga tatggatcca
    22261 acattaagaa aaacaacctc gtctaaaagc gtagccgaaa acggttaggt ttttttagtt
    22321 tgcaccgaag ggaggtggaa aaatgaaaaa agtgacgtta agcggcgatg tcgtggataa
    22381 cgataccgcg tggctttatg actggtttgg gatcaattgt atctcaccag ggaaaatttc
    22441 tgccgctctt acagaagcag cgggggatga agtagaactt gatatctcat cgaacggtgg
    22501 ggatgtccta gcggcaagcg aaatatatac cgctatccgt gcctatccgg gaaaggtatc
    22561 cggaaatgtt gtgagtattg cagcaagcgc tgcaagtgta atcgcttgcg cttgcgaacc
    22621 gcttagaatc tcacctacgg cacacatcat gattcataac gcatgggtga ccactagtgg
    22681 caacgctgag gaattaaaag ccaatgcaga aatgttaagc agtgtggatg agtctattgt
    22741 taatgcttac gagatcaaaa caggactcga tcggaaaaaa cttgctgatt taatggcgaa
    22801 agatacttgg ttaaatgctc aaacagcagt agcagaaggg tttgcagatg aaattatgtt
    22861 tgcagaagca ccagtaacgg tactcaatgc ctctcaaccg gttattccaa aaaacgcagt
    22921 aactaagttg aaaaatttaa tactcaaagc ggaagcaccg caagaagaaa cactcttaca
    22981 gaaaaaacta aaagccttaa atggagggaa aaacgaatga atttagaaca attaaaaaat
    23041 gcgtgggtcg aggcgggaag taaagtctct gacttaaatg cacaactcaa tgcagcattg
    23101 gttgacgatg aaaaaacaga agaagatgta gtaagtttgc aagcacaagt aaaagcagca
    23161 cgggctaaac gggacggatt gaaagagcaa gtggcaaata tggaagccga acaagtctta
    23221 aacgtcaaaa aagaaccatt agataaaaaa gatgaaaact tgaaaaacaa gtttatcaaa
    23281 gactttaaag cgatggtcaa tggtgatcct gctattatgg ctactttgac atctaatacg
    23341 gatgaatctg gtaatgctat cggattgact attcctgtag atgtgcaaac gactattcat
    23401 actttggttc gtcggtttga ctctttgcaa gaatacgtaa acgttgaaaa agtgaccact
    23461 accagcggtt ctcgcgttta tgaaaaatgg tctgatatta caccgttgac tgctttggat
    23521 actgaagacg gtgaaatccc agcaaatgat gatcctgcac tttacttgat caaatacttg
    23581 atcaaacgct atgcaggtat ttccacagta actaacagct tgctaaaaga tactgccgaa
    23641 aacattttgg catggttgtc taaatggatc gcgaaaaaag tagttgttac tcgcaataca
    23701 aaaatcttgg cagctattga tggaatcaaa gcggcacaaa agaaagatgt tacagatgtt
    23761 gatggaatta aagatatcgt aaacgtccaa cttgatccag ctatcgaagc tacatctatg
    23821 tttattacaa accaagatgg cttcaatgtt ttagataaag tgaaacgtgc tgatggatct
    23881 tacttgttac aaaaagacgt agcttctgca actggatata ccttcttagg taaaccaatc
    23941 aagaaaattg cttctcgttt cttgccaaac aaaggtacgc aagctactcc taaatatcca
    24001 ctgtacattg gtgatctgaa agaagccgtt acattgtatg atcgcgaaaa catgagcttg
    24061 ctgacaacga atattggtgg tggagctttt gaaacagaca ccactaaagt acgcgtcatc
    24121 gatcgcttcg atgtgcaact agttgatgat gaagcggttg ttttggctac ttttacaact
    24181 attgcgaacg agacaccggc gaaagtttaa ggagctgatt ccttatgatt cttgatccta
    24241 aaatggattt aggcgaaatc aaaaacgcac taaagattga taccgatgat gacgatgtgg
    24301 aagtaagccg tgcggcacaa gctgcaattg catacattag aggggctatc gggaatgata
    24361 agccctcttt ttataagcaa gaaaatgaaa cggttgatct gattaattta gctatcctgc
    24421 aattagcaga tcattattac aacgctcgat cagcaaccgt gagtgggaac ttgcgagagt
    24481 acgatttagg ttttacaagc ctaatcttgc aactcaaagc aagttatttg ctttttgtgg
    24541 aggaggagta gcgtatgccc cttatccaaa caggaaattt aaatcaacgc atcaagtttg
    24601 tccgagatac gacggttaag gatgaggacg ggcaagttgt cccgacttct acaaccattc
    24661 ttacttgctg ggcaagtgtg cagacacaac gcctgaacga tattaagacg tcgattggta
    24721 cggctttgga aggaacactg acgttcatta tccgctacca acaaaaatca gagctgacca
    24781 atgatatgaa agtgcgttgg aatggaaaaa cgtttgaaat cattacgatt acgaaaggcg
    24841 agtttgcgaa ggacttcacg acaatcattg caaaagaggt ttcaaaatga gtgtagaagt
    24901 cgatgcaacc gaagtgtaca aagcgcttag ggaagtaaaa gcaaacgttc aacgagtgga
    24961 aagcccagca cttagaaaag ctggggagta cgctcaagaa gagttacgac aaaacacacc
    25021 ttactgggat ggaacgaagt caaacggtaa acgtggttcg tatatgcaag aacatgctaa
    25081 gaaccatgtg gttacaagct cgataaaaaa cggattggta gaagtcggct atgacaaaga
    25141 tgtttcttgg cggatgcact ttatcgagtt cggaacaatc aaacaacgtc caaaaggttt
    25201 cgtacaaaaa acacaaaagc aaatcgaaaa acaagtaaca caaatcattg ctgacgaagt
    25261 aaaaaggagg ctaggacttt gaaaacggca gtatcacaag tctattcaat tctgaatagc
    25321 aatgaaaaaa caaagaacat tgatttttac accaatagtg ttccggaatc agctcaaaca
    25381 gtacctagcc ttccagttgg cagaattaca gagatatccg gcaactatga agattttgca
    25441 agcaacaatc ctttgaccat tcaatttaac gtacaggtag atgtatgggt gtcaaccatg
    25501 aaagaggttg atgcctttta ttttgccctt gatgaggtta tgagggggaa tggttggcaa
    25561 tgcgcataca cggaacaaac agatgacgag gacttggaag gtgcaaagcg gattatcaaa
    25621 cgatatgtag caaatatttc actaaattaa aaggagagaa aatagatggc aacagtagga
    25681 ttcgagagcg tcatttttgg cgtaaaaaca ggtgcaggcg gcactctaaa agaattagta
    25741 gcagataagt cgaaaggcgg agcgatcgaa gctaaaatta ctggattagg cgcaacttct
    25801 aacacaacat acgcttcaaa cgtaccgttc ttcattgcaa gtaaaggggt ttcgtcgcca
    25861 aaagttacgc ttgacgtggc agacttaatg gataacggca tttacagcga aatcattggt
    25921 gctaaaaccg tggatggtgc aaatgtaatt ggttcagaaa ctgaagcgcc ttacgtgtcg
    25981 gtagtcatgg ttacagcgaa caaagaagga aaacgcttgt tcatgggatt gacaaaagga
    26041 aaattcagtc atccagatat cgacatgaaa acagctgaag acaaaggggt agaattgcaa
    26101 accgattcca tcgaagggga attcatttct gatgaacgtg gctatgtata cttaacagcc
    26161 gtagaatcag aaggaatgac cttacaaaaa ttcaaggact tggtaaataa caaagagggg
    26221 gagtagttaa ccctgcatct acaccaacga cagatacagg gacaccaaaa gaaccagaac
    26281 caaaaattga tacacaaggt tagccatttt tggctagcct tattttttgt aaaaacaagg
    26341 aggaaaacaa atgattgaat tgcaattgaa acttgacgga aagaaaaaaa cattcaaaca
    26401 acaagatatt tccgcacgtg caatgcgtga gtgtatcaaa ttttacgaga aagcggaaaa
    26461 agcagaccta actgatttag aagcgattga ttcaatgatt gcaattacag cagatatttt
    26521 ccaagatcca gcagttacat ttgatgctat tttagacggt ttgactgcga gcgagttagt
    26581 accggcatta gaaagtgttt ttgaacaaat aaatgaactg ggaaacaatg aaaaaaagca
    26641 gacggcgagc aaaaagaaat aagtttttct gaagctagga aagcaatgga tcaaatctac
    26701 aaagatttaa tcgaagcagg ttggacgatg agagatgtgg acgaagccga ctatcattat
    26761 ttgttacacc tttttggaga agtggagagt ggcgaagaat atgtagatgg tgctgatttc
    26821 atcaaacaat ttttatcggc tgaagactta gtaaaacttg aggaaggagg taaataatgg
    26881 caggaaaagg acaaccggca ggaaatatca agctagggat tagtttagat agcactagtt
    26941 ttggtaacac gctggacgaa atcaatgcga aagtcaaaca agctgagtcg aatatgcgtg
    27001 ccaatctaaa ggcttatgat tcagcaggac gttcatacga agcacttagt caaaagacga
    27061 aagacttgtc tacggttatg gaagggcaaa acgccaaagt aagagaatta acaaagcgcc
    27121 gtgatgaagc gattagcaag tatggcgagg aatcgaaaca agttgctaac cttaacacac
    27181 agataaacaa tgctaccgca aaatataatg cttacagtcg ccagttgaac gacacaaaaa
    27241 aagaattggt gtattccaaa acagccgtca atgatttatc taatgaaatc aaagaaaatg
    27301 aacgacaaat gaacgccgaa gtaaaagcgt tgaaagccgc tggtgatgaa tctggtgctt
    27361 ttgaagcaaa acaaaaaggg ctagccaaac aaacggaatt atccgagaaa gctatcgaag
    27421 aacagcgcaa agttgtgaaa ctgatggctg atgagtttgg cgattcagca aatgaaaccg
    27481 aagatgcaaa aagggcatta gaaaagttag aacgacaaag ccaaatatct agcaggcaat
    27541 tagaagcact caaaagctcc agcgatcaat caggaaaaga aatagaagat tttggcgaca
    27601 agtccacaag gtcagctagg aaactggacg gactaaaaga caaattaagc tcgctaaaaa
    27661 gcgcattttc gtttggtgca gttgctggat tagcgcataa cgctattagc agtgtagtaa
    27721 gtggcgtgca aggtttggtt ggcgaagcag taaacgcatc ggattcattg atgaagtttt
    27781 ccaaaaccat ggagtttgct aactttggga agtcacagat agaaagctcg aaaaaagaaa
    27841 tgaaagacta cgccgataag acggtttatg gtttagaaga aattctgaac acaaccgcac
    27901 aattggcatc taatgggatt cctaactata cagaactaac caaggcggca ggtaacttga
    27961 atgccgttgc aggcggttct agtgatacat tcaaatccgt tgccatgatg ctaacgcaga
    28021 cggcaggagc tgggaaacta acaactgaaa actggaatca attagcagat gcgataccgg
    28081 gtgcttcagg attgttacaa gacgctatgt tgaaaaacgg agcttataca ggaaacttcc
    28141 gtgatgcaat ggcgcaaggt caaatcactt ccgacgagtt caaccaagca attgtacagt
    28201 taggtatgaa tgacggagca gttaaggcag ccacttctac agacacattg agcggttctt
    28261 gggagcagat gaaatccact gtaataaatg ggctacaaag tattatagaa aaaataggcg
    28321 ttgaaaatat cactggtttc atcaacagag taacaaaagg gattgaaaat tctattccta
    28381 aaattactca atttataggt tggttgagag atattggaac gtggatcgtt gaaaatagag
    28441 agccactaac atggattgtc ggaatcatag gcggaattac attagcagta aaagcattga
    28501 acgtagcaag tatgttgctg gcaattactg gcggaacatt ggcagcccct tttgtggcga
    28561 ttggtgtagc attaggcgca ctagcaggtg ctttggtgat agcttataca aaatctgaaa
    28621 catttagaaa tatagtcaat gcggctttta cagctgtgaa aaacgtagtt atgagcgtta
    28681 tcaataattt ggtggaatac tacaaaatgt tgtggagcgt gttgcagtgg ctttgggaaa
    28741 aaataaaaga atgggcttca tggattggca ataaattcat tgaaatgaag aacagcgttg
    28801 tgaatacggt tcagaaccta tggaatggcg tgaaaaactt cttcagcaat ggcgttggag
    28861 atacgtggaa taaggtagtc ggttgggtaa aaaacatttt caacaaagca actgaattga
    28921 agaacaacgt ttctgatgta atcggtaacc tgtggaatgg tatcaaagac acattccgta
    28981 gaggtatcga tacggtattc aattggtttt cagaactacc aacgaagatg aagaatgcca
    29041 ttattggcgg taaaaacgcc attgttgatg cgttcaaaag tattttcaac gcagcactta
    29101 aagcgatagg taaaccagtt aacgcaatca tccatggagc ttcatgggta ctagaaaaat
    29161 tgggcgcaga acctctacaa gaatgggatg taccacaata cgctacaggt acaccagcag
    29221 gtgggcaccc aatcaatggt ccaatgatgg ttaatgatgg acgtggagca gaaacagtta
    29281 tcacaccaga tggtagagcc ttcattccta aaggacgcaa tgttgttcta aacgcaccaa
    29341 aaggaacgca tgtcttgaca gcagaagaga ccgcgcaact tcaaggttct aaagctccta
    29401 agtatcgtta caaaaaaggt actaacttct ttggtaacat gtgggatagt gtgaaaaagg
    29461 ttgctggtaa tgtaggtcac acaattaaaa acgtagtagg tgacgtgtgg gactttattt
    29521 cagacccggg agcgttagct agaaaagtac ttggggggtt agatgtatta ggtggattga
    29581 caaagtatcc attagaagta ggtaaaggca tcctatctaa agcaacaagt gcactgactg
    29641 aaaaaatcac tgggttgttc tcatctggta acttagatac ctccgtagga acaaatggcg
    29701 tctataaata tttggctgat gttgctaagt ctgtgatgaa gaaattccca ggatttgtgg
    29761 caactagtgg gtatagacca ggtgaccctt attcacatgg taaacgtaat gccattgata
    29821 ttgcattacc aggcgtcaca ggaggctcac ctagatacac agaagcagca aactacgctt
    29881 ttgacaaatt cgcttccaaa attggttacg taatcactaa tgggaaagtt cgtgaccgtt
    29941 caggacaatc aggtcaacca gcaactggtg catgggagcc atggcccgat ggagatcact
    30001 atgatcatgt gcatttaaac ggtgtgaaag acccacaaaa cactcaaatt tcaggagata
    30061 gcgtgggagg cagtggggta gaaagatggc gcaatgtagc aattagagcg ttgaaaatga
    30121 ccggtcaata cagtactgca aacttaaatg cattactaaa tcaaatgcgt acagagtcaa
    30181 atggtaatcc taatgcagtt aacaattggg atattaacgc caaaaatgga acaccatcaa
    30241 aagggttgct ccaagtgatt gacccaacat tcagacagta tgcaatgcca ggattcaaca
    30301 gcaatatttt tgacccacta tctaacatct tagcttcaat cagatacgca ctatcaagat
    30361 atggctcact aacaaatgcc tatcgtggag ttggttacgc aaacggtgga attgtaaacc
    30421 aacatcaaat tgcggaaatc gcagaaggaa acaagccaga aattattatt ccgttagata
    30481 aggctaaacg atcaagagcg atgcaattat tgtctatcgc aatggacaaa ctaggagttt
    30541 ctcctaaaac ttatggaaat gcgacaactg tttccagtga ttacgaaatc ctagaagcca
    30601 ttgaaaaaca ggcggcaaca acgaatcagc tgttatcgtt attgctggca tttttcaaag
    30661 gaaatagccg aactgataga gatttagctt tagatattca gaagatcttg gttaggagga
    30721 tgtagatgcg aactgtatta ctgagaaata gaataaacga agaaattgat ttgtccacag
    30781 aagactattt tgctactggg ttgagtaata tggggtttga agtaaaaaaa gaacatgtgg
    30841 gacagtgggg gaatttcaga gaaagcagtg agagcgttga aatatctgaa tttcagtcat
    30901 ctgtaatcat ttctgtgcat gggtttcgag aaaaagaact gtacaattca cttgtgcagt
    30961 tcctatcgga aggtccgttt gaactggaat ttgcttttga cggcgaaaca atggtaagaa
    31021 gatgcagtct aaaatcttta agtaagactg aaatcgatcc gaaaacatcg ttactaacag
    31081 acactttaga actatatttc acatcaaact ggtattccgt aaaacgcgaa aaattaattc
    31141 agcgaccgaa tgtagtgaaa acacgtggta aggtttttcc gtataaaaga tcttatatct
    31201 acactcaaaa cttgtgggag aaaaaaggtg tattcaaatt caataataat tctgtatacc
    31261 taacgaatag caaggaacgg atgtccccgc taaaaattcg tgtgattggg aagtgttcaa
    31321 atccgtattg ggaagtaatc caaaactcac aaatcatcgc aacggatgga tacttcatag
    31381 atatgactga aacacagact ctagaagtat caagcctttt tgaagataca acggcgattt
    31441 taaaagatat tgcaggggta gaatcttctg tctaccaaca acaggattat acaaaaacta
    31501 attttgttca ggccccaact ggagaattca gcattgtgtt tcatgttggg ggggcagacg
    31561 tagagattga gttatacgag gagcgtgatc tgttttgatt ttagcagtca cgctttttca
    31621 tcgtgattta atgctttata acgaacattt attctctaac agttttgaat ttggtgtgga
    31681 cgagatcaac gaagaggcta gtagctttac gatggataag tacgttcctg taaaaacagg
    31741 cgattttcta ctagcaaaat atattcctag tggaaaattt gcatattttg gtgtaattac
    31801 gtcgcaggaa gacgaaaaaa tcagttgtaa aagcttactt agtttagctg atagtgagat
    31861 accgactgca cgtgtgtcag gagataacta cgaagagcat atccggcggt tgattgaata
    31921 ctatttgctg aatgatccaa cgaaacaact aaaagatatt ttagacgtca aagcagaaag
    31981 tgcgacctct cattcgtatc aagctaccga tacgaataaa cacaagttaa gtgcgtatat
    32041 tctcaatggt ttcaaaaaat acaacgtaaa atggtatttc aagggaattc aaaacagaaa
    32101 aatttataca ggcatacgtg ctgtaaatga atcaatttac attaaagaca attcttctga
    32161 atttagcgat tgggatgtgt ttgttcaggc gccgggcgct ggaaacgaga acaagctatt
    32221 aatcgttgat aaagcaatga aagatataga gaaaccgata atactgtcaa catggtattt
    32281 ggacgaagaa aacaatttga cacaagatgg atcgaaagaa aatatcacga aaccaactgt
    32341 caatttggtt aatatctacg atcaaaccgc agaagataaa gcatcctacg aagatgtggc
    32401 aaaatcagag ttgaaaggca atacgtattc gcatgaaatc aaagtgaacg ttgtaagaaa
    32461 tgcaaaaaat ttgaatgtcg aaacgattga aacagggatg tttgccacga tttcttataa
    32521 aggaaagata tacaagtcgg ttttgacagc ttggcgaata tcaagtgata aggagtttgt
    32581 ggaattgact ttcggaaaca taagaagtcg ttttatggat tattttgaag ataatggggg
    32641 ataaaaaatg gttagcaatg tggatggata tcaatttgaa aacgtgaaag taagcgcaga
    32701 aaatgatgct agactttatc acgttttata taatcggaaa aatcaggtta ttgatggtta
    32761 cgatcagtct atgaatttat cttcaagcgg attaacagta aaagttgctg caggagcagc
    32821 gattattcaa ggtcgtatgg tcgttgttcg acaagaagaa agtataacag ttccagcaaa
    32881 ctcaagtggt tatatagcat taacagtgga tttgacacaa gaagttatac ctggatctat
    32941 tcttccagaa tcggaagaat atgaatggac taataatcaa gtcaagctag aatttataac
    33001 aaaagttata aaaggtaatt taaacaatgg tgataaggtc tataatttac cactatgctc
    33061 agttaattct actggatcaa ctgtttcaat ctcaaagata tcggatagtt acgagctgac
    33121 tctctctaaa ggagaaattt tgtggagggg gactgcgtta atgcatgata ctcaaactgt
    33181 ccaaccttca aaaaaaattt ggcagacagt ttcaggtttt ttgttagtat ggctcccata
    33241 cgaaaacggg caagcaattg aggatagata tgtaactacg cctttttata aggagcgtgt
    33301 aacttttact aatgttttag gagaaattgt ttcaggattt gatgactatc acaaaaagtg
    33361 gtttagtaaa cgaataaact ataattcgaa tactaatata tttacaggtg cagcaagcaa
    33421 tgcaagtgga gataacgcaa atatggtgct taggtatata gtttcttttt agttaggagg
    33481 tatggaagag tggcaaattt agaaattaaa ttatctgcaa ataagagaca gccttatcta
    33541 cgtcaccgtg ttgttggtag agttggtgat ggggggctca caacaatcaa tgtacaactt
    33601 cttgaggaag atgaaattac accttttgta attaatctaa acggtacttt gaaatttgtg
    33661 ggcgaagttt caaacggtaa ctataccgag ggagaaccag aaataatcga ttcgactaat
    33721 gggttaatta gttacacgtt cactaagtca aatttcagca cgagtcatca attcaaacaa
    33781 gcatattttg aatatgtaga tcctaacggc aaaaaagtaa cttttcagaa cttcatcata
    33841 gacgtgttag aacgagtgga tattaattcg gagcaagcga aatactatat ttcttcattg
    33901 gaaaaattac agagtgaaat gcaaaccact ttcaatcagt tcattagtga taaacaggtc
    33961 caatacgatc aaatctactc gaaatataac gaattagtaa gattgataaa tgaatcagat
    34021 aagcaagtaa atgatcgtat tgatcaaacc aatcagcaaa tcggcgatct cggcaagctg
    34081 aaaaagatgt acagtaacag catcgacttt gggggctatg attattcggg gaatccgaat
    34141 ttgttaagta agctatcata cgacttaatt gaaaatcaaa atacttcagc tggaacactt
    34201 tctaagggtg aaaactcgtt taaatataat aagatatcag ctgaagtgga aggtggagta
    34261 gagttatatt ataaacgaag aggtatagct aactggttac cctctaataa aacgcttgta
    34321 atgactgtta agcttagagc tggagcggac tatagtccag ttgacggaaa acttatactg
    34381 attagatata ggtatgttga cagtggaact ggcaagattg ttttagactt acctattaac
    34441 agtaattcga taactcagga atggaaagag tttagtatta ctggaactac tccaacattt
    34501 agcccacaag cataccatcc ttggatacaa tttagggctc aagatggggt acttggggaa
    34561 atagaaatga gctatgacat caaaatcgaa gaaggctcta cagctacacc atatcagcct
    34621 aacttacttg atgactctta ctggctaggt aaaacaccgt tgggtgagaa tttgataacg
    34681 aatgccaagt tcccaattat aacacgaagc aatcctattt ctggttttga tatttcagaa
    34741 gaattaataa taggtgaaac ttatacggta tctttaaaag ggactaagcc agctactaaa
    34801 gaattccact tatactacgg agaagcaaat taccagcagt tgcagtatca ggcaacttta
    34861 cttcctgtgg aaggattaac agatgtgtgg agtgccactt ttacagccag aaatactagt
    34921 gaaacgacaa atttacttag agtagcgtta tggcaaaaac ctaattctgc aacatatgat
    34981 actgttcaaa ttgactggct caagattgaa aaaggcgaca cacgcacacc aaatatcagt
    35041 cagtttaaat actttggtga aggcttgaaa gacagtaaca atccgaacga ctacagctgg
    35101 gatgtcacac cagagtatac tgaaaaaagc ttgaataata cggttagtct gactgaaccg
    35161 caatccatcg aaggattgaa aaatttcgaa gatggtattc aatcaaaagg aaaatctgta
    35221 ttgacatcag acgacaacaa atatgaagtc gtaaccttaa cagttacaaa cgggaatacc
    35281 ggatcagcaa agctttatcg tgaaggaaaa accgtcacta tttatttttt tgcgttgaat
    35341 gggaaaagca gtggtggaaa tgattcaacg atactaacaa ttccagaagg ctatcggcca
    35401 ccaattagtt ttgagcaact ggttggctcg atagaccgtt ctactttgaa cagtgctcag
    35461 ttatctattg gtgcagatgg agccattaaa tggcgaagaa actcaagtta tggatcggat
    35521 tatacctttg caattactta cacgatttag aaaggcgtga atcgatatga aggcagcata
    35581 tagaccaatt gaaccttacg gattcgagca aatcattgtg aatgatgaag aacatttacc
    35641 ggaagaatgc acagaagtcg aaccaccgat tccaaattgg aaaccgaaat tcaattactg
    35701 ggagggaaat aaatgaaaaa catttggaaa tatggccgta ctggcggaga gtatgcagga
    35761 aaagtattgg acgacatgct tgtatccgtt ccttacacag atcagccacc gctcgaaggg
    35821 gttcgttcag atggcgaacc gctaacgatt gctgatcaga tgtttgatcc taaattgaac
    35881 caatggatta ttttagcgaa cgcgttagat cacaacgatt taaacaatct caaagcgatg
    35941 tacgaggctc ttgaacatga aaacggcaac ctaaaacagc taaatgccaa actcatgcta
    36001 agcgatgtag cgattaaaca ggaaaatact gcattgaaag aaaaagctga cagtttagca
    36061 caaatcaatt caaaaatgat gcttgcttcg ttacaaaata gcaaagacat ttcagaaatt
    36121 aaagagcaac taaatccagc ttcaaaggga ggtgagtagt atgtttagtt ttagcgatgt
    36181 gaaaatgatg tatgattggg gctgttttac tgacgatcaa gttcgtctat tcgttccact
    36241 atgtatcaca gatgaagaag cagatagaat tattagcaaa gaagagagcg catcttaagt
    36301 gatgcgtttt tttgttggaa agttggtgga acatgaaaga agaagcgctc caagacgttg
    36361 tggagagatt agtaagaatt gaaacaaaat tagacaacta cgaatcactt agagaaaaag
    36421 ctgatagtgc aaaagatttg gcagacaaag cctactcagt agcgctaaac aacgcagaag
    36481 atatcaaaga aatgaaagcc aataataaat ggtcgtgggg ttacatgatc ggtttaggca
    36541 ttacgatcat tggctatttc ttgactaaat tgtaaaggag ttaagaagaa atgattttac
    36601 ccgataagta ttatcaagtc attaaatgga cggttttaac agtattacca gctgcatctg
    36661 ttttagtagc cacgttagga aaagcgtatg gatggaatgg aacagatatg acagtactca
    36721 ctatcaatgc agtagcaaca tttttaggcg ttatcactgg tgtgtctgca tataacttaa
    36781 aaaaatagga ggaaaaaaat gaagaagaaa atcattttat cattgagcct aataatggct
    36841 ctttttttat tgccaattaa cgggttcgcc tatacgatca acaatgaatt taatttaggt
    36901 gtaaatgaag gtagctcaca agtagcaaat aaccaataca tcctattgca tgaaacagca
    36961 aacgaaactg cgacaggacg aaatgaagcg cagtacatga aacgttcttg gtacaatgcc
    37021 tatacagcgt atattgttgg cgatggtggg atcgtttacc aagttggaca acctggttat
    37081 gtacaatatg gcgctggttc atatgctaat gcaaatagtc ctgtgcaaat cgaactgcaa
    37141 catacgcacg ataaagtaac gtttgaaaag aactacaagg catatgttga attagcgaga
    37201 gattccgcta ataaatacgg tattccactt acattagaca caccgtataa tcaaccagga
    37261 atcaaatcac atttatgggt aactcaaaac atttggggcg atcacactga tccgtatgga
    37321 tacttatcag aaatgggtgt aagtaaggaa aaactggctt acgatttagc tcatggattt
    37381 acagacgaaa acccaacgac ttctgagaac aagcctgtca ttgatccaac ccgagcaggt
    37441 gcagcaaatc caactttatc agatggaacg aaccattctc acattgatca gtttggagaa
    37501 atcgaaaatg cgaacttaca cgtcgctgga tggcacatcg ctaactataa atacgagtat
    37561 attttcatta tggactacaa tactgggaaa gaattagctc gagtaagagc tgatggaatt
    37621 tatagaccgg atgtaaatca agcttataat acttctggaa acgttggcta tcatgtatct
    37681 tttaacatgc gcaatttccc taataagaaa gtatacgtca tgatgcgggc aacgaatgat
    37741 ccagaaggaa acactaaagg cggtgcgcaa gatttccatg acaaacgttg gtatttaaat
    37801 attcctaaac gataaaaata gctcctcgtt gaggagcagt acatagaatt gaaaattaac
    37861 gttaaatcaa aaaaatattt acaagatgtt tatttaagta gataataaaa gaaaaaagca
    37921 ggtgaaattg tgacagcaga aattggtatt atgaataaaa gtggaatcgt gttagcttca
    37981 gattcagcat ctacgattgg agatagcaaa gtatataata ctgcaaaaaa attatttact
    38041 ttggattcca tgcattctgt aggtatcatg atatatggta atgctgaatt taatggtatt
    38101 ccttgggaaa taatcattac tcaatataag aaaagcattg gtagttctgt ttttaatact
    38161 ttggaagaat acgcggataa ttttatagaa tttgtcaaaa cagcatcttt tattagaagt
    38221 gaacagaccg agcaagaaca aatgattggg gtttttcaaa aaataatttc aggtttattt
    38281 gagagtatag aagtagatat taacttttta ataagccaag gtacacaaat agacaaagat
    38341 gtcctagtga agttattaca aacaaaaatg aataccaatt tgtctcaaca gtcccagaca
    38401 ttcattttag atattgaaaa ggcgttattt cttagtaact atggagaaat attaaaagat
    38461 attcttaata gtatttctac aatggaaggt gtttcagaag ctatatcaga ggaaattcaa
    38521 agttacgttt atgaaattat aattagagac gatgtttatt cttcccctac aggaatagtt
    38581 atagcaggat atggaagaat ggatattttc cctaaactat attcttataa tatgtttgga
    38641 tttgttatga atattctgaa atactcagaa tatgaaagtg ctcaaatagg aaatgacaat
    38701 ggttcattaa gatctactat attaccattt gctcagtctg atgtggtaaa tactgtagtt
    38761 caaggggtag atccacaaat tactaattat ctgtcgagtc aagtcgatag ttttgacgat
    38821 aacggaaaaa atacctatat taatattata aagaatattt cagagtttca acaaaatcaa
    38881 ttcatatttc cgttactaaa catgattgct ttactcccag ttgaagaaac agcaattatc
    38941 gcagagactt tattaaattt aaccagtttt aaacgaaaat atactacttc tgtagaaaca
    39001 gtaggtggcc caattgatgt tttagctatt acacctaatg atggccctat ttggattaaa
    39061 agaaaacatt attttgatat tgataataat attggataca gattgcgaaa ggagagagcg
    39121 aatgattaca ataattaaac ctcaggaaaa atcccctgtt ttgaaattta atgagagcaa
    39181 tctagcccat aataaaatga gtataactga tacagtattt gatgtaatgg ttgagcaaca
    39241 aaaaaaaatt aataataaca atagacaaag aaaagaaatt aaccaatatt aatgtttttt
    39301 ctgttgcgtt ttttgcagtc ataatttaaa acctcgctca atcttgggcg aggtcttttt
    39361 ttatttttct gaaaagttaa agtaaataat agcataaaat aatatatttt acaaagaata
    39421 agtacaatct agttttttgc tattaaatgt gtaataataa atatgccatc acaacatgaa
    39481 gaatgaaatc cattattatc tagtctagtc cattcttttt gtttgcagta tttgtgatgg
    39541 ctttc
//
LOCUS       DASNNU010000017        37799 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.017, whole genome shotgun sequence.
ACCESSION   DASNNU010000017 DASNNU010000000
VERSION     DASNNU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.017"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..386
                     /locus_tag="U8116_000824"
     CDS             <1..386
                     /locus_tag="U8116_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEN8433838.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(691..1005)
                     /locus_tag="U8116_000825"
                     /pseudo
     CDS             complement(691..1005)
                     /locus_tag="U8116_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(1259..2894)
                     /locus_tag="U8116_000826"
                     /pseudo
     CDS             complement(1259..2894)
                     /locus_tag="U8116_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3217..3696)
                     /gene="rlmH"
                     /locus_tag="U8116_000827"
     CDS             complement(3217..3696)
                     /gene="rlmH"
                     /locus_tag="U8116_000827"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEN8433839.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4124..5413
                     /locus_tag="U8116_000828"
     CDS             4124..5413
                     /locus_tag="U8116_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEN8433840.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(5452..6057)
                     /locus_tag="U8116_000829"
     CDS             complement(5452..6057)
                     /locus_tag="U8116_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEN8433841.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(6262..6726)
                     /locus_tag="U8116_000830"
     CDS             complement(6262..6726)
                     /locus_tag="U8116_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN8433842.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(6748..7068)
                     /locus_tag="U8116_000831"
     CDS             complement(6748..7068)
                     /locus_tag="U8116_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN8433843.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(7212..8291)
                     /gene="pepA"
                     /locus_tag="U8116_000832"
     CDS             complement(7212..8291)
                     /gene="pepA"
                     /locus_tag="U8116_000832"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEN8433844.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            8491..8841
                     /locus_tag="U8116_000833"
     CDS             8491..8841
                     /locus_tag="U8116_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN8433845.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            9064..10011
                     /locus_tag="U8116_000834"
     CDS             9064..10011
                     /locus_tag="U8116_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN8433846.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(10068..11486)
                     /gene="pepV"
                     /locus_tag="U8116_000835"
     CDS             complement(10068..11486)
                     /gene="pepV"
                     /locus_tag="U8116_000835"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEN8433847.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            11635..12480
                     /locus_tag="U8116_000836"
     CDS             11635..12480
                     /locus_tag="U8116_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEN8433848.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            12484..12924
                     /locus_tag="U8116_000837"
     CDS             12484..12924
                     /locus_tag="U8116_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEN8433849.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12974..13168
                     /locus_tag="U8116_000838"
     CDS             12974..13168
                     /locus_tag="U8116_000838"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433850.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            13304..13990
                     /locus_tag="U8116_000839"
     CDS             13304..13990
                     /locus_tag="U8116_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN8433851.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(14398..15087)
                     /locus_tag="U8116_000840"
     CDS             complement(14398..15087)
                     /locus_tag="U8116_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433852.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(15062..17191)
                     /locus_tag="U8116_000841"
     CDS             complement(15062..17191)
                     /locus_tag="U8116_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEN8433853.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(17239..18576)
                     /locus_tag="U8116_000842"
     CDS             complement(17239..18576)
                     /locus_tag="U8116_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEN8433854.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLELV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(18828..19187)
                     /gene="rplT"
                     /locus_tag="U8116_000843"
     CDS             complement(18828..19187)
                     /gene="rplT"
                     /locus_tag="U8116_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEN8433855.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(19240..19440)
                     /gene="rpmI"
                     /locus_tag="U8116_000844"
     CDS             complement(19240..19440)
                     /gene="rpmI"
                     /locus_tag="U8116_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEN8433856.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(19512..20036)
                     /gene="infC"
                     /locus_tag="U8116_000845"
     CDS             complement(19512..20036)
                     /gene="infC"
                     /locus_tag="U8116_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEN8433857.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(20080..20205)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            20356..20988
                     /locus_tag="U8116_000846"
     CDS             20356..20988
                     /locus_tag="U8116_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN8433858.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(20990..21895)
                     /locus_tag="U8116_000847"
     CDS             complement(20990..21895)
                     /locus_tag="U8116_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN8433859.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21980..22639)
                     /locus_tag="U8116_000848"
     CDS             complement(21980..22639)
                     /locus_tag="U8116_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN8433860.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            22955..23899
                     /gene="mvk"
                     /locus_tag="U8116_000849"
     CDS             22955..23899
                     /gene="mvk"
                     /locus_tag="U8116_000849"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEN8433861.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23892..24869
                     /gene="mvaD"
                     /locus_tag="U8116_000850"
     CDS             23892..24869
                     /gene="mvaD"
                     /locus_tag="U8116_000850"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEN8433862.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            24882..25967
                     /locus_tag="U8116_000851"
     CDS             24882..25967
                     /locus_tag="U8116_000851"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEN8433863.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            25964..27028
                     /locus_tag="U8116_000852"
     CDS             25964..27028
                     /locus_tag="U8116_000852"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEN8433864.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(27277..28080)
                     /locus_tag="U8116_000853"
     CDS             complement(27277..28080)
                     /locus_tag="U8116_000853"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEN8433865.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(28100..28942)
                     /gene="kduI"
                     /locus_tag="U8116_000854"
     CDS             complement(28100..28942)
                     /gene="kduI"
                     /locus_tag="U8116_000854"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEN8433866.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(28958..29965)
                     /locus_tag="U8116_000855"
     CDS             complement(28958..29965)
                     /locus_tag="U8116_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEN8433867.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(29972..30616)
                     /locus_tag="U8116_000856"
     CDS             complement(29972..30616)
                     /locus_tag="U8116_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEN8433868.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(30716..31474)
                     /locus_tag="U8116_000857"
     CDS             complement(30716..31474)
                     /locus_tag="U8116_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEN8433869.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(31601..32752)
                     /gene="fucO"
                     /locus_tag="U8116_000858"
     CDS             complement(31601..32752)
                     /gene="fucO"
                     /locus_tag="U8116_000858"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEN8433870.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(32834..33145)
                     /gene="rhaM"
                     /locus_tag="U8116_000859"
     CDS             complement(32834..33145)
                     /gene="rhaM"
                     /locus_tag="U8116_000859"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEN8433871.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(33147..33992)
                     /gene="rhaD"
                     /locus_tag="U8116_000860"
     CDS             complement(33147..33992)
                     /gene="rhaD"
                     /locus_tag="U8116_000860"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEN8433872.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(34006..35262)
                     /gene="rhaA"
                     /locus_tag="U8116_000861"
     CDS             complement(34006..35262)
                     /gene="rhaA"
                     /locus_tag="U8116_000861"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEN8433873.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(35259..36719)
                     /gene="rhaB"
                     /locus_tag="U8116_000862"
     CDS             complement(35259..36719)
                     /gene="rhaB"
                     /locus_tag="U8116_000862"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEN8433874.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            36872..37741
                     /locus_tag="U8116_000863"
     CDS             36872..37741
                     /locus_tag="U8116_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEN8433875.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
BASE COUNT        11026 a         7915 c         6877 g        11981 t
ORIGIN      
        1 atcaattaga aagcaacttt tttcgggatc taaatgaaat tctctcttat gatccagccc
       61 atagctatga ggaactatac actcgcttga ttgactatgt ttacgcaaat acttttttat
      121 atcgcttatt tatcctcaac catggtgata tttcgtttca gaagaaagtt aaacaagtta
      181 ttgaagaaaa ctatctgaac atatggctat atgaagacaa gaaaacgatt atcacagaag
      241 aaatgagatt ctttactacg taccacgtcc aaggttgtct atccgttctc aatcggtggg
      301 tagaaagcaa ttacaagtac tccaaaaaag aattattaga actcttacag aaactgaatg
      361 atcacataga gaaaatcatg ccttgaataa aaacatacta agcttagtta agaagaaccg
      421 taaccttctc gtataacaac tgaatcttat ttagccaaat tgtttactga agattttttg
      481 agtaaaattt ctataattcc tacttattga aaagtagttt gtagtgctgc ttctccctct
      541 ctaagtgata tatcttgttg taccaatcaa attcacctca tcattaataa attactttta
      601 ggctaattta ttacaactta taggttgaag ctgtaaccaa tttatagtca tattatattt
      661 ttatcatcca gtttattgaa ttcttctcgt ttacctcaat tcattttcaa ggggaatcag
      721 ctcatcatct aacattttac gccatgctcg cttcatttct tgtgggtatt taaatggttt
      781 cgcttctgga tgtttggctt tatatccttc gtaatcctta ctgttgataa ttgctttcat
      841 attaggaatt ggatcttcac ctggcatata ttgcagatta gaagcatgta attttttctc
      901 agaaacaaac cactcattgg aaaagacatg aatcaaatca tcacttactt gtgtaaaata
      961 gcgacgttta atgacaaaga tatcttcatt gctatctagt gtaccttcat ttctttgtgc
     1021 ttaaattcaa agataatttt ctcaataaag atttttgatt ccagtaagat tcctttactc
     1081 cttttacaac atagcgttgt cctttgtttt gaaacatctt cttcccctct ttgccattaa
     1141 aaatagccca attctctata aagaagatta agcttcatta ttataatata aagtttaaat
     1201 ttggtttatt acttactttt tcttattctc tagtaagcta atattgaaaa aaatgtaatc
     1261 agatcattat ttttatatgt tgtaacttcc tgataagaaa actaattaaa agaaagaggt
     1321 aactacataa actaattaaa aaagctaact tcatagttgg aggataaatg gaagaaactt
     1381 caatgattgt ttctccttgt gttccttcaa tcgacactag tcctattatc aatctctctt
     1441 ttttgtccat ttacttttgc gactgttccc caataatcaa atacaggaag cgttattttt
     1501 tgtcctttag attcatgtac tgtatagact attttcgaat ccatcacttg ataaggcata
     1561 gtatgccatt ccttgccaat tcttacttca tgattctgca aatgttcttt gtattgtaat
     1621 gcttccgtgg gtgcatagtc ataaagtcct ccgggaagga gtgtcgtgta acgaataaga
     1681 ttttcttgat tcattaccaa gtgatctctc tgagtgacaa ctttatgttc atagtttgct
     1741 tttgttgcag cagatacagc caacagtaat aaaacaattg ccgttatatt aactactttt
     1801 ctttgcttag ctgatgtgct cttcatgaaa acgaacgata aaatcaacga gccaaacagt
     1861 acttgaaagc ctaagattcg ccaaggaaat tgaatcattt gaatgggtgt attttgtaat
     1921 aaagaccatg gaaaaaattt ggtggataag agccaagaga ctaccgcact aaggtaaatc
     1981 actttttctt tgcgattaaa tcctttaaaa aagaagagtg gacagatcaa agaaataata
     2041 ccaatgattc ctaccgtaaa ggttgtcagg tcattattca tcgcaccaat caaactatcc
     2101 gagacattta atgcttggtg ttgtaaggtc gttttatctg gctcattaat cgattgaacc
     2161 atgatttgtc ggaacatcgg ataccaaaag tatgctgtta aaagaatcgt cattcctact
     2221 gcctttgaca actccacaat caaccggatg ctttcttttt ttcttgtttc acttactaca
     2281 aagccgccta agactagtaa gccaacggct attacactca taaaagtgga aagaacatgt
     2341 gtataaatga gtaagctcat actgactgct aatggaattg cacatggctt tttctcataa
     2401 atcagtgcgt aagcataata cataactatc ggtataaccg taagtgcaat cgcttcagca
     2461 atagcagaac gatgataaat atcaatcgta cggtacccat aaaatgtata taaacagcta
     2521 aatataaaag ccgcatcgat tctttttaaa aattttagtc cataatgaaa catcagcatg
     2581 atagtacaaa cattcaatag ccaaacatat aagatataac taacgattaa attattactt
     2641 atccaataaa aaatgactgc cggaaaaaag gtcaagtagg gataaaaata attcactccg
     2701 ctcccataat gattaaacgt cgtaaaattg attggaccag aaaaaattga agataaccct
     2761 ttgactctag caaaatggaa ggcaatatcc cccgatgtag aaatagtatg aacatttcta
     2821 aaataattga ttgaataaag tccagcaata attgctagga gaatggacaa tattattttt
     2881 ttctttactt gcattactta attatatact cctttcatgg attgctttat ctagtataac
     2941 ttaaatttta aataaatcgt tatttatttt tactctcact taaaaaagca ctaaaattat
     3001 aagagaggga ggtaatggtc tgaacaccaa gaattgttcc aacatcgaat tgcttaacta
     3061 atgagttcaa agcttaatta tgtggctctt atatttaccg agcgtggatt gcgatctctt
     3121 tagcgaaaca aacatctttc ataaaaaaaa cagccatatc cgctttcttg gatatgacta
     3181 tttactacta ctttactatc tttcattctt cttgtctcac ttatgataag gcgcccccgc
     3241 attgatccgg aaggcacggt agatttgctc gacaaggatc agacgcatca attgatgtgg
     3301 atacgtcatt tttccaaaag agatctgagc attgcttctt ttcatgacgg cttcgctgag
     3361 tcctaaagag ccgccgataa caaaaacaag gtggcttttt ccttggatac ctaattggtc
     3421 gatttgttta gcaaaagatt cacttgtagg atttttccct tcaattgcta aggcatagac
     3481 gtattcttgt tctttgattt tcgctaagat ccgttcccct tctttttctt ttacttgtct
     3541 catttgcgct tcgcttaaat tctccggggc tttttcgtca ggaacttcga tcaattcgat
     3601 ttttgcatag gcattcagcc gtttgacata ctcattgata ccttggatca ggtatttttc
     3661 ttttaatttt cctactgaga taattttgat attcatgttt tctctccttt ggtcttcatg
     3721 ctcactttgt ttattataat caattttttc gtctatatgt ttattttggc tatttttgtt
     3781 aaaaagattt ccccaacagt tatgcacaac atccacaggt ttatccacag aaaacagtgt
     3841 tattttacct tatttcatca cattcattag gcaaacatac gttttataag aatttatcca
     3901 ctttatacac aggctgtgga tatgttttat cccttatttt tccaatccta tccgcgtagt
     3961 tttacacagt atctgtggaa aaagaaaatc aaatattttc atctgaaaac aatgattttt
     4021 cctaaagttt ttcataaaaa gttcacagtc tcaaagtaga ctaaagtcaa caaataagac
     4081 tatactaaca gtagataaat gaatgtaatt ggaggaggaa cacatggata gaaaaaatgt
     4141 gacaccaaag atgaaaaaaa gtaaaaataa tagcctctgg cgtaagttag gtctggggct
     4201 tgttggaggg atcgttggag gactactgac agctggcatt ttttatgcag cgatgggcac
     4261 tggcaacaat agttctactg ctacaagcgg caatcagaat tctgccggtg aaacagttgt
     4321 ggaaaatgtc aaagtcaatg tagattctga tatcaccaaa gcggtagata aagttcaaga
     4381 cgctgtcgtt tctgttatca acttacaaag tcaaaaccaa tcaagcggtt ttggcggact
     4441 attcgggcag caagaagaaa gtagcagctc agatgatagt aatttggaag cttatagtga
     4501 aggaagcggc gtgatctaca aaaaagacgg gaatactgct tatgtagtca cgaataatca
     4561 cgtagtagat ggccagcaag gtcttgaagt cttgatgaaa gacggtacga aggtaaaagc
     4621 tgaattagtt ggaacagatg cttattcaga tttagcggtg ttgaagatca attcagacaa
     4681 agtcgaaaca gttgcctctt ttggtgactc aagtgctctg aaggttggtg agcctgcgat
     4741 tgcgattggt tccccactag gatctgaata tgcaaattct gtcacttcag gaatcatttc
     4801 ttccttgaac cgacaagtga caagtacgaa tgaatcgaac gaaacagtca atatcaatgc
     4861 gatccaaaca gatgcagcaa tcaaccctgg aaattctggt ggtcctttag ttaatatcga
     4921 aggacaagtt atcggtatca actcaagtaa gatcgcaagt acttctgaat ctacttctaa
     4981 tgtaagcgta gaaggaatgg gatttgctat tccaagtaac gatgtagtga atatcatcaa
     5041 tcaactggaa aaagacggaa aagtgacacg tccagctcta ggtatcacaa tggtcgactt
     5101 atctgccgtt tctactcagc aacaagaaca aatcttgaag attccttcat ctgtaactaa
     5161 cggtgtcatc gtgacaaatg tccaaacagc tacaccggct gaaaaagctg gattgaaaca
     5221 atacgacgtc attactaaga tcgatgacaa agaagtttct tctggtgtag aacttcaatc
     5281 tgctttgtat aagaaaaaag tgggagattc tgtcaaaatc acttactatg aaggctcgaa
     5341 agaaaaaaca actactgttg aattgacgat cgatcaatcg gcattaaaac aaaatcaaaa
     5401 tagtgaaaat taataaaaaa gaggttgtga caaagacttg tcacaacctc tttattttgg
     5461 aaatgcttct ccgactacgg catcctcagg caatactaag attccttttt ttgctggagc
     5521 atttggcaaa tgcaattcac gagcggaaca gatcattcca tagctatcta cccctcgcaa
     5581 agttcctggc cagatcatca ttccatctgg catcatagct cctggttttg caacgacaac
     5641 tttttgtcca gcttcgatat tgggtgcacc gcaaacgatt tgcaatactg taccattgtc
     5701 tacttctgtc tcagtaatcg ataaatggtc agaatcagga tgtgggacgc atgtttttac
     5761 gtacccgaca acgatcttcg gttcatgatc agcttcaagc tgttcttcga atcctgcttc
     5821 tttgatcaat cgattcaatt tcgctaattc ctcatctgtt aagcttactt ggccgttccc
     5881 ttcgattgtt agatgatcgg agatatggaa aaagttccaa gcaacaactt gaccttgttc
     5941 atctttgatc cgagcaacat tccatttgcg ttcaacagtg ttttctgttc ctttatcgtc
     6001 ggctataata accatcaagg tgtctccgac gtgtgctttg ttataagcaa aaatcatgtt
     6061 ctttcctcca ttatcctttg ctgagattta tcttaacaaa aaattgcatt cactgcatga
     6121 aatttttttc atgctttatt tccgaagaag cctcttctgg aacaccattt attcgtaaca
     6181 agggactgtg acaagacttt tgtcacagtc cctttactgt tttagttaat tgattaaaaa
     6241 ggtcgtacaa gttccacttg cttaattact tggctgttct tcttcgtcag aaacgatcaa
     6301 gacatcacaa ggtgaatgac gtatcacgta gctggctaca cttcctgtca taaagcgttc
     6361 gactgcattt agacctgact gcccaaccat gattagatcg atctcatatt tttttggcaa
     6421 tttgactgcc atggattcac gaacagaacc atatgcaata attccttcta tatttgtgta
     6481 atcaacagat ttcccatatt ctttacattc ttcgatcatt tccttggctg tttcagtttg
     6541 ctgatcgact agattttgat caataggtgc atagcccata aagttataaa attgctgatc
     6601 gattacgttt gctacatata ctttcccatt attacggcga gccacttcta ccgctttttt
     6661 gaaagcagca agtgcttgtt cactaccgtc gatacctact aaaatattct tatattcttg
     6721 attcatcttg acccctccta acagatgtca gccttttgtt acactttcta ggaacgattc
     6781 gatttcctgt tttgttttac gatctttatt cactagacga cctaattctt ttccgtcttg
     6841 gatcacgaca aaacttggga taccaaaaat gttccattca gcggctacat ctatgtactt
     6901 gtcacggtct acttcaataa attgccaatc agaaaagtca ttttcaattt caggcatttg
     6961 cggtttgata aaacggcagt ctccgcacca gccggcagta aagaaaaata cactctttcc
     7021 agtttctacg tagcctgcta attcttctaa actatttggg ataatcattc tttgtcctct
     7081 actttctgtt tatgtatttt tcaattaaat tatacacctt tctttcgaac taacaaacat
     7141 tgatcctctt tttataaaaa aaatctggca aaaagcaatc ttttgccaga ttttatagtt
     7201 tcactatcgc attattttcc gtatacgatc gtatttactg ttgatttatc caaacctttc
     7261 agtgtttgaa tcaacatttc tcgtgcagct tcaaaatcgc gaatactgaa catcgtttga
     7321 tgcgtatgga tataacgtcc cactacgcca atcactgtac ttgggattcc ttcgttttgt
     7381 gtatgagcag cacctgcatc tgtaccgcct tttgaaacaa agtattgata agggatattg
     7441 tttgtttcag cgatatccag caagtactcg cgcaaacgag gaagcatgat caaacctgga
     7501 tcttggatac gcatcaatgt accttctcct aaatgtccga atgtgccatt tttcgtcact
     7561 gtatcatctg ctgcagagca gtctactgca aagaacagat ctggcttgaa tttattgaca
     7621 gaagctttag aaccacgcaa gcctacttct tcctggacat tcgcgcctgc aatcaatgta
     7681 tgacctaatg tttcattttg caacgcttct aaagcttcta ataccattgt acagccatag
     7741 cggttgtccc atgatttact gataatattc tttccgttgg ctgttttgat cgtttcagcg
     7801 taaggaacga ttgtgtctcc tggaaggaca ccgtaactca ttgcctcttc tcttgattca
     7861 aacccagcat cgaacaaaat atcggttact tctactgatt tttgtccgct tgttcctcgc
     7921 aataaatgtg ggggaacgga agaagaaata catggataat tcttaccagt agaggtttta
     7981 agtgtaaaac gctgagcaga aacaacgtaa ggattccagc cgcctagagg tacgacttta
     8041 aaaagcccgt tttcttggat ttgtgtcagc atgaatccga cttcatccat atgtgctgcg
     8101 accattacac ggggtgcatc cgcttcttca tgatggcgca gaccaaaaat gccgcccaaa
     8161 ccatctaact gtaactcatc taccagcggt tccatatgac tttccatata agcgcgaaca
     8221 tcttgttcaa aaccgcttgt tccttgtaat tctgttaact ctttgattcg ttgaaatgtt
     8281 ttttcttcca tatgctattc gtctaaattt cagactaaac tcctctcaga cttatattga
     8341 tctgcggata aaatatgatt ttttctgcag accgatcgta tttattatag cttatttttt
     8401 ctggtagcgg ttatttattt caaagaaaat atgagagtgt ggtaaaataa gcacaaggag
     8461 taacaagtga gaagaaggag gtatgtccac atgtatgaag aaagcgaact aagctatttt
     8521 aaaggcgggt tagcagttgg aatcggtcta ggattattga gcggggtagc ttcaacgatt
     8581 tggtatcatc gcaaaaaagt attgtcagca gaccaagtac ttgaaaatat caagtcatct
     8641 tttctaaaag aaggtgcaat cgaaggttct tggatctctt ttgaaaagga acctattagg
     8701 aaattcgccg ttcggtcgag tgcttatcgg ggaggtcttt ctcgaataga agatggcgaa
     8761 actgtttatt atgaattttt agcagatgct catacaggaa ccgtattaga aatttcaaga
     8821 aaaaaaggtg caacagatta atacgtcaaa aaagtgagag aacgaatacg cacccactat
     8881 tcaaaagaga ggtatcatgt ccttcttttg tagtgggtgg ttttttagat agagctttcc
     8941 aaacatatat tccagtattt tctttttaaa taaatgatat ttagcgggaa aaactagcat
     9001 ttcctttgat tatgaggtat ttttaattta gcttttgttt tctatagtta aggagagtga
     9061 aaaatggaac aacaagaaaa aattgctgtt ctgattcctt gttacaatga ggaagcaaca
     9121 atttcaacag tcattgcaga ctttaaacga gaattaccag aagcagatat ttatgtctat
     9181 gataataatt cgaccgatcg aacatacgaa ttggccgttc aaggtggagc aatcgtaaaa
     9241 aaagaacctc gtcaaggaaa aggaaatgtg atccgccaaa tgttctttga tatcgatgcc
     9301 gactattatt taatggtaga tggcgatgat acttatcctg cagaagctgt acatggtttg
     9361 ctcgagaaat tacgttctgg tgaagcagat atggtcattg gtgatcgcct atcaaatgga
     9421 acctactttg atgagaataa gagacctttc cacgattttg gaaataacct tgttagaaat
     9481 acaattacta gaatgtataa aacaaaaatt cttgatgtca tgaccggcta tcgaggattc
     9541 aatcgtatct ttgtcaaaag ttttcctatt atgagttctg gttttcaaat cgaaacggaa
     9601 ttgacgatcc atgctttaga taaaaaattc aagttcgttg aaatcccaat cgattatcgt
     9661 gatcgtcctg aaggcagcga atcaaaattg aatacattct ctgacggatt caaagtgatc
     9721 atgatgatta ttaaaatgtt taaagattat aagcctttgc ttttctttgg gatttggacc
     9781 atcttcttct ttttatttgg attggtcacg gggattcctg tgattcgcga atatatgctg
     9841 acaagcttta ttacacgcat cccttctgcg attttaagta ctggattgat gactttagca
     9901 cttctttcat tagtaacagg gttgattttg gatacagtcg tcacgaacgc taagaaagaa
     9961 tatgaattga atctatatca tgtttatcat gaataccaca gtagaaagta aaaaaaggat
    10021 caagactgca aaaataattt gcaattcttg atcctctttt taattaatta tttcgccaaa
    10081 cgatagatgg catctgcata gattgctgtt gctctgaaca agtcatcaag actcataaat
    10141 tcgtttgctt gatgcattgt atcagtatat cctgggaaca tcgctccata agcaacgcca
    10201 cgtttcaaca aacgtccgta agttccgcca ccgatgattt gttcgtagcc tttttctcca
    10261 gtgtggtctt catagacttg caataatgta gccaccaatg gatcatccat tggtacgtaa
    10321 tgtggttcca ttacgcgagc acctcgagta actgttgcgc cttcttgccc cactgtttgt
    10381 tcgatgtcac tttgcaatcc gtctactgtt acaccttttg gaaaacggaa attcatattg
    10441 atgaagttcg cttcctctgt accattttct tcaaaagcaa atagtccagc attcattgtc
    10501 aatttgccca ttttttcatc ttcataagcg actcctagtt tttctccata aaaatcttca
    10561 tggacataag cagctgctac atggataaat tgtttagcag atcctaggaa ttcatagtta
    10621 tctaagaaca atgccaagaa agaacctgca ttgataccag attgtgggct tgcaccatgt
    10681 gcgcctttac caactaattc gatttttaca tacgtattgt ccgcttcaat cgttccgctt
    10741 actggatttg cttcgatgaa ttggtaaaat gcttcttcca tcgcgatagc tgcatctgca
    10801 cttggtactt gaagtgctgc agttgctgtt cctggcacca tattttcgcg aagtccggaa
    10861 acaaaagatt tcaagacata atctcctgca ttgtcgcctt tgaagcgtaa agctaaagaa
    10921 acatttcctt tttcaccatt gatgattggg aattcagcat ctggtgagaa accaaaatct
    10981 ggttcttctt catgttcgaa ataataagcc atatcgcccc agccgctttc ttcatcgctg
    11041 ccgactacga agcgaacttt ttttgatagt ttcaagtcaa gttctttaat gatcttcatc
    11101 gcatagtaag ctgccatact tggacctttg tcatcgcttg atccgcgtgc aaagatcttt
    11161 ccatccttga ttactggttc ataaggatct gtgtcccagc catctcctgc aggaacgaca
    11221 tccatgtgtc caaatatccc tagcgtttcg tctccttcgc caagatcaat atgccctgca
    11281 tagttatcga cattttttac tttaaatcca tcacgttcgc cataagctaa catatgtttc
    11341 aatgcgtcgc gaggtccagg tccaaatggt gcatctggtg ttacttttga gtcatcgcgc
    11401 tcgctgttga cacgtaaaag atttttcaag tcttcaagca agtcttcttt acgtgcttct
    11461 acttcttttt gccaatcaat tgtcataaag ttaatcctcc acttatttaa gtcttcgtgt
    11521 tcatgatacc acaaattaca gaaaaaaata gttcaaaaaa tctgacaatt ccttttctta
    11581 tctgtttttc tctaaaaaaa atatacgcta taatgagtaa aaggaggttt ttagatgctg
    11641 ataaacgaac aactagtgaa aagagtcatc acacctcgtc caaaaatttc acataaagga
    11701 acatttggac gtatcgtttt gattggcggc aatgaacaat atggcggagc aatcattatg
    11761 agtgctttag ctgccgtaca cagcggagcg ggtctcacga ctgtagtaac tgatgaaaaa
    11821 aatcggagtg ctcttcacag tcatctgcca gaagcaatga ttgtagtttg gacggatact
    11881 gaccagtttc tttccgtaat aaagcaagca gatgtactct tgattggtcc cggattgggt
    11941 acttcttcta agagtttgga acgtttcaaa caagtactag aaaaccaatc aaaagaacaa
    12001 tggctggtga ttgatggctc tgctttaagt ttacttgctg ctaatcatct tttgctaccc
    12061 tatcccgaac aaactgtact tacacctcat caaatggagt ggcaacggtt gagttctcta
    12121 ccgatacaag aacaacggga taaagaaaac aaaaagaagc aacaggaaat cggtgctgtg
    12181 ctcgttttga aaagtcatga gacaaaaatt tatggtcacc agcacttcta caccaatcct
    12241 ttaggttctg ctgctatggc aacgggcggc atgggtgaca ctttagctgg gatgatcaca
    12301 ggatttttag cccagtttcc tcaaaatgac caaacgattg cagcagctat ttatcttcat
    12361 agtttcattg gagaccagct ggcaaaagaa caatatattg ttttgccgac acaaatcagc
    12421 gaagcacttc cgaaatggat gaattatttt tgtaaaaaat caatggaaaa cgaggcatga
    12481 gtcatggaga aatttacagg ttatgtgcga gaaccttttt attatgaaac agatcaaatg
    12541 gggattattc atcacagcaa ttatatccgc tggtttgaag aggctcgggt agccttattg
    12601 gtacatctag ggtttcccta taaaaaaatc gaagaatctg ggatcatcat ccccgttctg
    12661 gaagtaaatt gcagctataa agaaatgatc cattacggag aaaaagtgag catccatcca
    12721 actatccaaa agtatacggg gacacggttg gattttagct acgaaattgt gggaactgaa
    12781 gacaaaaaat taaaaactac tggcagtagt aaacactgtt ttttactttc tgattcgcaa
    12841 cgattagtga agctgagcaa agtcaatccg gagttagatc agttattcca agcgtacgct
    12901 tctttggatg atgtaaattc ttaataaaga ccgtctaagc atttttcttg tattgtgtcg
    12961 agtagttcgt ataatgaaaa gaaaaaagca ggtgatcccc atgtatgtcg ttaaagtaat
    13021 gcacggttac atcgataaaa ctggctgtcg cactcgggaa aaaaacttag acaatctgct
    13081 cattttcaaa gacaaaaaag aatccgaagc ttttgctaaa agaatcggcg gtcgtgtcaa
    13141 accgatccaa gaggtacgac cagattgatt caatattttt cttttcgagg ctctaatagg
    13201 catgcctatc agggcttttt ttgcagactg tgtttttatg cccacacgat ttgtaagcga
    13261 tttatgttat aatagattat atctgcatag aaaggaaggt taaatggtta tcaataattt
    13321 gcaacagcaa gcttatgaat ctatacgtaa acaaatcatt tattccgagt tagcacctgg
    13381 aagtaaaatc tccgataaag tgttagaaga acaactagct atcgggcgta caccgattca
    13441 agaagcactg atccaactgc gcaatcaaga gcttgtaaaa accattccac agtccggcac
    13501 ttatgtttca aaaatcgata tccgatctgc ttcattagcc cgttatacgc gggaaaagtt
    13561 ggaaaaaccg atcctgcaag aatgcagtgc gaaaatgacc aagcaagatc aagctaaact
    13621 agaaaaaata ctagaacaaa ccgatcaggc agtaatcgct caaaataaaa aagctttttt
    13681 tgatttggat actgattttc accgaacttg ttatgaaatt gctggaaaac gagaaatttg
    13741 ggattggcta gaaagttata gtacgcactt gaaccgtttc agatggttac gtttgacgat
    13801 ttcagaatta gactggggtc gagtattaga tgaacatcag acaatgcttc aatcaatgat
    13861 tgatcataac tttgatgaag tcggatttct ttgcacgatg catttacata tgattatcga
    13921 ggagcaagaa tacgtgattc ataattaccc cgattatttt gaagatattc aggacagacc
    13981 catcaaataa gaggttttgg aatcagcgtt ttgacctgag tattagagaa agaaacggca
    14041 aaatagttcc tcatattttg ccgttttttt tgagctaata ccgagggtca gctgattgca
    14101 caccgtttat tgagaggttg tgaaaaagct gccctgcatc aagtaataag aacagccaag
    14161 taaaaatagc ttcttatgtt tttaacttgg ctggacttat taccgcagat gcaagctttt
    14221 gaacaccgtt ttccaagagg tcgtgagcct gccgttcatc tccgagtcac aaggaacaat
    14281 ttcaaaaaac agcttctcat gtttgttgaa tattttgact tgtgaccgag gagttgacag
    14341 gagaacatcg tttattcgga aaaaaaggac tgtgacacga cttttgtcac agtctcttta
    14401 tttgtaatct aaatcatact ccgtacatct tttttctatt ttttttattt cttctaagat
    14461 cccttggcta cgaatttcat tatatggcca aagtaagact gcctctaata cataaggcga
    14521 aaaatcgctt tgattcatct cttttgaaaa actcatcgct gctatctcgc tcaatgcacg
    14581 aatgtttgat tgatgtgtat aaaaaggcaa ccgccacagc acaatttttt cgctttgcgt
    14641 ataaattccc ttaacagaag cttcgatgtg gtgaaatagt tcatgtccaa taatcaaagc
    14701 ttgcgctgtt tctttagaga tcgaagctag tcctgaacat tttaattttt ctaacggctc
    14761 cgtcatcaat cgaatccctt catcttctgc ataagttgca aaagtgattc gttcgtttat
    14821 ttgctctgat tcacaggaaa cgattggaac agccatttgt tcacaaattt ctcctacatc
    14881 ggcaaaaggg tacttttttt tgatgctaca tgccgtttcg ataccacaat cgattgattg
    14941 ttgtataaga attttctgct tggacaaagg aatctttttc caaaataaat ctcgccgaaa
    15001 ctggtagaat ccccaaattt cttcggggat ctgttgtaat tcgtttagta actcttctaa
    15061 cttatgtttg tttgatattt ccggcaacga cgatcacttc ttcctccggt tccatagtag
    15121 atacttccag atccatcaaa agctgtagtt gttccagatc cgtcgctgta aaatcagaga
    15181 ctcttgctcc taagcagaga taaaattctg gtcgggcaat caattcagtt tgataaactg
    15241 ccatatcttc ccaaagttgt tcgacagctg tcatataatt ggctgcagtt gttttcatac
    15301 atgaagcatg tccacgttgg accttgataa atcctttttg atcgatgatc cgaagacttc
    15361 cagcattttt tgaacgattc tgttcgccgc atacataaaa gaaaggtgta ttttccaata
    15421 agcaaacttc atttttattt aatcgcatat cctctgcagc aagctctagt gcttcttctg
    15481 ttgtgatctc cttggttaga tcggttgctt tcacttccgt agaaccagta gcgatagccg
    15541 ttacttttga agtctgtgga tcgatttcta cgtggacttc gatcgattct ggtgtagcgc
    15601 ctgattcaat cgctttattc atcgcttcat ttttcaacga tcgtatatct tctttacttg
    15661 gcgaagggat gatacgttct acaacatctc ggaccattgc aagtgctaca ccaatagaag
    15721 aaatcacttc ggcgttttca ggaatcgaat atttcacacc cattttatta ctaaagtaag
    15781 taatcaatga ggcagcgccg ccccctacac ctacaagaga aatctgatct ttctccaagt
    15841 gatacttgtc tgccaattct aaaatcaccg gttcaatctt agcataggat ttttccatga
    15901 tctgttctgc gatgtcttca accgtcgtat gacaataatc agctaatgct tggatggctt
    15961 ttcgtgcaga tgggacattt ccataagaaa aatgttcttc ttgcactaat ccaaggacat
    16021 tggctgcaca tgtatttgta atagtcactt cttctccgtc ttccatcact accttgacgt
    16081 aatcagcagg gtctccttct ttaggagaga aaaattcaac cttaggacct ttgatttttt
    16141 ctgtctccgt aaatactgca taatctagac cggcaatatg tgcagaacgt ggaccgacat
    16201 cgatgattcc tgactgatta gcccgtacca tcgaaccgcc tgccacacct agaacccgaa
    16261 catctaatga agagatatat gttgggtggc cacctacaat tgaataatcg attgctggac
    16321 gtccattttt aatgaccccg atattggttg ttgttccgcc tacttcaaaa taaacaccat
    16381 tagacgctct caagtacatc aaagagccca tgactgatgc tgctggaccg gaaagcatgg
    16441 ttaaaacagg tcgttttttc atttcattga tttccattac tccgccgtct ccgcgcataa
    16501 tcatcaaaga aacattgacc cctgcttctc gtacggaatc ttctgtagag gttgccgtat
    16561 ctagcatttt tggtaaaata gatgcattga ttgctgctgt ccgagttctt cttgtcaatc
    16621 catataattt tgtgatgtca gaagccatcg ttgtagggat cgattttacc gatgctgctt
    16681 cataaacaac tcgttctggt tccccattat ctactccaaa tgccatagaa gaaaccaaga
    16741 cctccgctct ttctctttct aaagaagaaa tcgtttctga aacacggtct acatttaagt
    16801 gtttgacagg taagaatgca ttgacaatct ctattttttt cttattccct aaatcgatat
    16861 cgttcaatct agtttgtcgt tttgctaaaa aaccttctaa tccacctttt gccatcccga
    16921 ttacgccgac ttttgctacg tctccttcaa tcagtgcatt agtcgcttgt gtggtagaat
    16981 gtgcaacaaa cacaacatct tctggactga tattattttc cagcaaacaa ttttgaaaag
    17041 actgaacgac acctgcggct actccacgga catcatcgtg ggttgttttc actgaagatt
    17101 ttccgatgat ttcgtgcgtc gcattatcga ttgcgactgc tttcgtatgt gtaccgccaa
    17161 catcaattcc cattcgcaca aaacgtttca ttttcttttc ctccattcat ctaatcgttt
    17221 ttatcgacgt tttgtttact aggcgttccc gtacaataag aacgtgatca ggcataagat
    17281 cgctccgatg atccaaccag ttgggatgga tttcttcata tattctcgag aagttacttt
    17341 tgtataacct aatccccaag caacccaaga ttgtgtaata tctaaatgct gaggagcaat
    17401 cgttgtgatt gcaaataacg ggaacaaaaa ggctggcgac atcgttggat taacagctaa
    17461 tacgacagct aaaatagctg agcctgaacc gactagattc attggaccgc ggaaaaatcc
    17521 tagtggtgtc aatatagcaa atacgattgc caagactaat ggtgattgcg gtatccaatc
    17581 tccaaaaata gctgaaaaat aaggagaagc atacgcagct gaattattga acatcgctaa
    17641 ggtcatcaag aaaccaacca ttggcgcaac atctactgcg ccatctgtaa ataatttcgc
    17701 aaactgacga cagatttctt tataacctcc gcgcaatttt ccagtaaaca gcaatgccca
    17761 gatacctgct agaataaaac ctaagatgat tggaagatct aaaagaacga cccctaaaac
    17821 tggtaataat acggctaacc atgagatcat tggtgcgtcg ccaccttcag ctggtacgtt
    17881 tgctgccatt gcatagcgtt tctttttgtt cattgatata ttagcgaccg ttagtaccac
    17941 aactaagctt acaatcatcc caatcatacc aatttggaaa tagtcattat aagtatagct
    18001 ttcagccgct ggattgaaac cagcataaat cgtttgatat tgtttgaaat tgacgatatt
    18061 agcaaagatt ccagccataa tcgagcccat aaatgagaac agtgcaaccg gtactgaaat
    18121 acctaatgac atcaagattg gcaacacaat aactgcaata gaaatcactg gtccgattcc
    18181 tgtcatcgac atgaatataa cagcggttac aatacatagt aagctcatcg tgattcttgg
    18241 cttgtcccct ccaagttcaa ctacctttcg aatcagggta gctgcgattc cgctatctac
    18301 tagaacacgc ccgaaaaagg caccaaagaa gacatttacc aagatcgatt tggcgtactc
    18361 agctggcccc gtcgtataga tatgcgtcaa cacatcaatt actccttgcc cttccatcgc
    18421 cgggtttggt gaaaacgtgt ttccgactag ctctaatccc atccatacag tagccatgat
    18481 ggcaaagccg accatcaagt tgtagccttt tacacagtac cagaccatga aaaagaatga
    18541 gataactaac aaaataccaa taataatatc actcatgtta ttcctccgtc cattttgttt
    18601 ttctgataag caaactaata tatcagtcaa actcattcta acttatgtag ttagcgcttt
    18661 cattaaaggg tgaccataaa tgttcagttt tcaattaaaa aaccttaatt atgctatttt
    18721 agacttatac gttattgtcc tgtttcattt gatggaggat tgtaaataaa aaaagaggaa
    18781 agaacataac ttttcgttat cttctttcct ctttataatt gtttagctta tttagccaaa
    18841 gcttcttttg cttggtcagc caatgcagta aatgctgctg catcgttaac agccaagtca
    18901 gctaacattt tacggttgat gtcgatttca gcaactttca aaccgtgcat taattttgag
    18961 tagctcaagc cattcatacg agctgctgcg ttgatacgag caatccacaa tttacggaag
    19021 tcgcgtttct tttgacgacg atcgcgatat gcgtagttgt atgaattcat tacttgttct
    19081 tttgctgttt taaataaagt gtgttttgaa ccgtagtaac cttttgctaa ttttagcact
    19141 cttttacgac gtttacgagt tactgttcca cctttaacac gtgccatgta taattcctcc
    19201 taataaaaat aaatcttcag attctgttat ttgtacttct tatttcattt ttgctagttg
    19261 ttggcgaata cgtttgaagt cgccactaga aaccatgctt gctttacgca attgacggcg
    19321 ttgttttttt gttttgccgt ggaaacggtg acttgtaaac gcgcggaagc gtttcaatcc
    19381 gcctttacca gtacgtttga aacgttttgc tgatccgcgg tgtgtttttt gttttggcat
    19441 gactatattt cctcctcaaa atctttcgtt atccgactat cggctgtcca acagccagtt
    19501 agtcattcaa attacttact gtcgtttttc ggtgccagcg tcaagaacat gctgcgtccg
    19561 tccattttcg ctttttgttc cactgttgca atatccgcag tttcttcagc taagcgattc
    19621 aagacttttt gaccaatttc tttatgggta atggcacggc ctttgaaacg gatagaagct
    19681 ttcactttat cgcccttttc caagaactta cgtgcattac gaagttttgt attgaagtca
    19741 ttcaagtcga tcgtaggact taagcgaact tcttttacat tgatcacttt ttgtttttta
    19801 cgcgcttcgc ggtctttctt ttgttgttcg aaacggtatt taccgtagtc catgattcgc
    19861 gcaactggtg gtttcgcacc aggggcaact aacacaagat ccaagtttgc ttgttctgca
    19921 atattcaatg cttctgcttt tgtcttaact cctaattgct caccatctga accgatcaat
    19981 cgtaactctc gtgcacgaat gccgtcgtta accatcatat cctttgctat ggtcattcac
    20041 ctccaaatat tatgagagaa agaaaagctt accgaataaa cggcaataaa aaaacgagtt
    20101 ctcttacaga acccgcgcgt acttcaccta atagaaccag tgaaaactat ctagcccagt
    20161 gacattatca actaaggcga gaagcgggtg cttctgcttt tatttctcaa ccatcatagt
    20221 atagcaaaaa ctaatctttt tgtcaatgtt tttcatacag tttgtcttcc ttttctgaaa
    20281 atgattagat tagcagactt tcttgcagtg attcccgctt ctttgctata ctaaagaaaa
    20341 acgaggtgaa cccacatgaa aaaaatcgaa cctattccac cttctcttcc tcttcttaca
    20401 aagggagagt tttatttaga agatgaggag atcttctctg gtcttctgat caacgggggt
    20461 gaccaaagct atctcgatgc caaaaatatg gtattaagag agagtcatat ccaaaaactt
    20521 acgatgcaaa aaacagtttt acgtcatttt gaatgcagta atgtcatttt tgaaaaatgt
    20581 gatttttcca atctggagtg gattggtggc agtttccacc aagtcgtgtt tcatcaatgt
    20641 aaattaacag gtacgaattt tgccgaaagt tatcttcgag actgtacttt cactgattgt
    20701 attgcttcga tggcttcctt tagtagtaca aacttgaaag ctgtctcctt cgatcattgt
    20761 caattagaag acagcgaatt ttatgaggtc acttggaaaa accttttttt gtctgacaat
    20821 cagttgaccg gttcgaactg gtttcgaact tctctgaagg gattagactt tacgactaat
    20881 ttcttcacag caatcgcttt atctcaagat tctttggcgg gactaatcgt caaccaagag
    20941 caagcacttg tgatcgccca agcccttggt ttgcagatca aagactagat caacgaatcc
    21001 gccgccacag tgcaataagc atccaaatag ataagaccaa actgatgatc aaggcgattc
    21061 cccatccgaa aacggaatgg gtaaagggta actggagatt cattccgaag aatcctgtca
    21121 caatagttgg aacggtcaag atcagcgacc agaccgtcaa aaatttcatc gtgtcattca
    21181 agttgttatt cagcaaatta ttataagtgc cagagagctg gtctaaaatc tgcgaagcta
    21241 agttcgtcat ttcaactgct tgtcgtgctt cgatcagcgc atcgtccaac tcttcttttt
    21301 ccttttctga caacttgcga taaatagcca atgccttgat ttgttctaaa agtactacat
    21361 tttgttttgt tcctgtaact aagtaaacca aaccgatctc taaatctgaa agttgcagca
    21421 gattcttgtt cgttgttttt tctcttaatt tcagattaag ccgctgacgt tcactattca
    21481 cttcctcgat taaaggaaaa aaggaatcag aaatcaaaaa caatgtacga aacaataaac
    21541 tgtatacact catgtctggc attcgttcca aaagcgatcg aattagtcga atcacgtact
    21601 cggtattcgg cgtggtaaat gtaaacaaac cgtcttcttt taaaataaaa gcgataggac
    21661 tagtttcata atgattatcg actttttgct ggtgcggaac atttgataca agcagtaacg
    21721 cttcatcaaa agaatcgtac tctacgcgcg cccgttcatt tttatctaga gaataagtca
    21781 gcatctcgtc actaatgcca tattcttctt ttaagttttt aacagctagc acatcgtttt
    21841 gctgtacatt gatccaaaaa ttacggtctt gattaaaata gtgtttttcc cgcatactct
    21901 atcctcctga catttgtttc cagtataaag aaaaaagtcg ctcaaataaa ggtttaccct
    21961 ttatttgagc aactgtaaat caaatggcta ctttggtttg gcgaatctct gctaattttt
    22021 cttcgatcat tttcaagact tcttcttttg cttgaggatc ttccacaaaa ttatagcggt
    22081 ctccatcgat ttggattttt ggactttctt tgtattcttc ataccattga tcgtaacgtc
    22141 tgttcaattc tttgtaataa tcgtatagac ttggatcaaa ggacaattgt tcatacgaac
    22201 gaccacgttt ttcgatacgc tccaacatcg tatcaaaaga tacacgaata tgaactaata
    22261 aatccggatg ttttttatgg gcagcatagg gaagttcttc catcatgttt tccagcagtt
    22321 catcatacac acgaacttct gtttctgttg ctcttcctag atctgcattt agatggaaaa
    22381 gcagtgaatc ttcataaatc gaacggtcca atacattatt gtcatctttc attgcttgtt
    22441 tgatgcttgc aaaacgttta ttcaaaaaat aaatttgcag caaaaaagca tactgttcag
    22501 ggtttgcata aaataagggc aagacttcat tatcatcaat cgattcataa aatgcctgac
    22561 tatcaaagtg ttcagccatc atttctgtta aacttgattt tccggcacca attgtacctg
    22621 ctaaaacgat cacgctcatt tacctccagc tcacttcatc actaaatatt gtgtttaact
    22681 ccaacctttt taaatatacc cccaatatct agtagtgtca attgtatttt tttctagtca
    22741 aagaagaaaa aaagtcaaaa aattcttaag aaaaaagtgt tttttcttta gaaaacgtat
    22801 tcttagtttt acttctgaga tggttttagt aaaataaagg aggaatgttt tttgagaccc
    22861 ttagtggaca aaaaaattcg tgaagcagcg caactcgttt tgggcgcttt attttttgct
    22921 taaaaatcac ttttacgtga gaaggagtta gattatggca aactatggcc aaggagaatc
    22981 aagcggaaag atcatattga tgggcgagca cgcggttgtt tatggagaac cagcgattgc
    23041 ctttcctttc tatgcaacaa aagtcaccgc attccttgaa gagctggatg caatggacga
    23101 tcaactggtt tcttcctact attcaggaaa tttagccgaa gctcctcatg cattaaaaaa
    23161 tatcaaaaaa ttattcattc acttaaaaaa acagcatgac atccaaaaaa acttgcaact
    23221 gaccattgaa agcacgattc ctgctgaacg tggaatggga tcaagcgctg cagtcgccac
    23281 agcagtcact cgtgcttttt atgattactt agcatttcct ttgtctcgtg aaatactatt
    23341 agaaaatgtc cagctttcgg aaaaaatcgc ccatggtaat cctagtggaa tcgatgcagc
    23401 cgctactagc agcttgcagc cgatttattt tacaaaaggg catcctttcg actacttttc
    23461 tttgaacatc gatgcttttt tgattgtcgc tgatacagga atcaaaggac aaacaagaga
    23521 agccgtcaaa gatgtcgctc acctctttga aacccagcct catgaaactg gacaaatgat
    23581 ccaaaaatta ggatacttga cgaagcaagc aaaacaagca atcattgaaa attcaccaga
    23641 aacgttggca cagacaatgg atgaatcaca atcacttctg gaaaagctga caatcagcaa
    23701 tgattttctt aatctattga tccaaacagc aaaagatacc ggggcattgg gtgccaaatt
    23761 aactggcggt ggacgcggtg gctgtatgat tgcattagca caaacaaaaa caaaagccca
    23821 agaaatcagt caagcacttg aagatgcggg tgctgctgaa acttggatac aaggattagg
    23881 agtacatacc tatgtttaaa ggcaaagcac gcgcatatac caacattgct ctaatcaaat
    23941 attggggtaa gaaaaatgaa gaactcatcc ttccaatgaa caacagcctt tcgttaacat
    24001 tggatgcatt ttatacagag acagaagtca tcttttctga tagctatatg gtagatgaat
    24061 tttatctaga tggcaccttg caagacgaaa aagcaacaaa aaaagtcagt cagtttcttg
    24121 acctttttcg taaagaagcc ggcctctcac taaaagcttc agtaatcagt cagaatttcg
    24181 ttcctaccgc agctggatta gcctcctctg ccagcgggct agctgcttta gcaggcgctt
    24241 gcaatactgc tcttaagctt ggattagatg atctctctct ttcaagattt gctcgacgcg
    24301 ggtctggttc agcttgccga agtattttcg gtggtttcgt cgaatgggaa aaaggccatg
    24361 acgacttaag ttcttacgct aagccagtcc cttccgattc tttcgaagac gatttagcaa
    24421 tggttttcgt tttgatcaac gaccagaaaa aagaagtgtc cagcagaaat gggatgcgtc
    24481 ggacagtcga aacatccagt ttttatcaag gctggttaga ttccgttgaa ggggatctat
    24541 atcaattgaa acaagcaatc aaaacaaaag atttccaact tctcggagaa acgatggaaa
    24601 gaaacggact aaaaatgcat ggaacgactt tagctgccca gcctccattc acttactggt
    24661 ctcctgacag cttaaaagcg atggatgcgg tgcgacaatt gagaaagcaa ggcattccat
    24721 gctactttac gatggatgct ggccctaatg tcaaagtttt agtggaaaac agccatttat
    24781 ctgaggtaca agaaacattt accaaattat ttagcaaaga acaagtaatt actgcacatg
    24841 ctggtccagg aattgcgatt attgaataaa gggagatttt tatgattgaa gtatctgcac
    24901 caggcaaact ctatattgcc ggagaatatg cagttgttga aacaggccat ccagcagtta
    24961 tcgctgcagt cgatcaattc gtgacagtca ctgtagaatc tgcacgaaaa gtcggaagta
    25021 tccaatctgc acaatatagt gggatgcctg tacgttggac aagacgcaac ggagaattag
    25081 ttttagatat tcgggaaaat ccttttcatt atatccttgc tgctattcgc ttgactgaaa
    25141 agtatgcaca agaaaaaaac attcttttat ccttttatga tctgaaagta acaagtgagt
    25201 tagacagttc aaatggccgg aaatatggat tgggttctag cggtgccgtg accgttgcaa
    25261 cggtcaaagc cttgaatgtt ttttacgcgt tgaatttatc tcagttggag attttcaaga
    25321 ttgcagcact agccaattta gcagttcaag ataatggttc ctgcggcgac atcgctgcta
    25381 gctgttatgg tggctggatc gctttctcaa ccttcgacca tccttggctc caagaacaag
    25441 aaactcagca ttctatcagt gagttacttg ccctggattg gccaggtcta tccattgagc
    25501 cattgattgc tcctgaagat ttacgtttat tgattggttg gacgggtagc cctgcctcta
    25561 cttctgattt ggtcgatcaa gttcaccgtt cgagagaaga taaaatggtg gcttatcagc
    25621 ttttcttaaa aaatagtaca gaatgtgtca acgaaatgat caaagggttt aaagaaaata
    25681 atgtaacgtt gatccaacag atgattcgaa aaaaccgaca attactgcat gatttatctg
    25741 caatcactgg ggtcgtcatc gaaacgcctg ctttgaacaa attgtgtaat ttagctgaac
    25801 agtatgaagg agccgcaaaa tcttctggtg caggtggggg cgattgcgga atcgtaattg
    25861 ttgaccagaa atctggcatt cttcctttaa tgagtgcatg ggaaaaagca gaaatcactc
    25921 cactgccgtt acatgtctat agcgatcaaa gaaaggaaaa ccgatgaatc gaaaagacga
    25981 acatgtttca ctagctaaag cctttcatga taaacaaaaa aacgaatttg acttcgtacg
    26041 gatcatccat aaccctttgc cgcaaatagc agtttctgat gtcgatctaa gtacacaggc
    26101 agttgggttt acactaagca gcccatttta tatcaatgcg atgactggcg gaagcgaaaa
    26161 aacaaaaaaa atcaaccaag atcttgcaat cgtcgcaaga gaagcagatt tgatgattgc
    26221 cactggttca gtcagcgctg ccttgaaagt cccttcttta gccgatactt atacgattat
    26281 gcgacaagaa tatcctcacg ggaaaatcat tgccaacatc ggtgcaggga cttctgtcga
    26341 aagagccaaa gaagctatcc gattgtttca cgcagatgcc ttacagattc atttgaatgc
    26401 acctcaagag ttggttatgc ctgagggaga ccgtgatttt accaattgga aagtacttat
    26461 tcaagaaact caaacagcca ttgacgtccc cctcatcgta aaagaagtcg gttttggcat
    26521 gacaagagaa acgctcaatg atctagctgc tttaggagtt catacagttg atatcagcgg
    26581 tcgaagcgga acaagcttca cccagatcga aaacgcccgt cgctccaagc gggaactgag
    26641 ttatttagct gactgggggc aatcgacagt ctcttcattg cttgaagcaa atgaagcaga
    26701 cacttctatg gaaatcctag cctccggagg tatccgcaat gcctatgaca tatttaaagc
    26761 cctttgtctc ggcgcaaatg ccgtgggtac ttcaggaacc gtactgacgc atctgatgaa
    26821 tcacggcgta gaagaaacta ttatactgat gaagcaatgg caagaagagc ttcgtctgct
    26881 ttatactatg gtcggcgcaa cgaataccgc ggccctacac caacaatctc tgatcttttc
    26941 cggaccagtg aaggattggt gcgaagcaag agggatcgac ttagtgaagt atggaagacg
    27001 cacagaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    27061 caaacggcgg aagtaaaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa
    27121 aagcaatttt cgtgaattcc ttcttatttc ttggagttaa atgcttctgt cccagcctct
    27181 tttttcgaat aaatgatgtt taatcagctg tccttcggta ttcgttcaat ccgctgattt
    27241 cacaacctct ccactaaaca gtgtactaaa gctcttttaa ggttgttttc cgatataggc
    27301 aagtatgcct ccgtccacat acaagacatg gccgttaacg aaatcagatg cgctagaagc
    27361 caagaagaca gatggtccag ccaaatcgat tggctcgccc cagcgtgctg ctggcgtacg
    27421 tccaacgata aattgatcga atggatgacg ttcaccgttt tcttgtgttt cgcgtaatgg
    27481 agcagtttgc ggtgtagcaa tataacctgg tccaattcca ttacattgga tattgtattg
    27541 accgtattca gaagcgatgt ttttcgtcag catttttaat ccgccttttg ccgcagcata
    27601 agcactgacc gtttcgcgtc ctaattcact catcatggag cagatattga tgattttacc
    27661 gccgcctttt tcgatcatat ccggaataac tgcttttgaa acgatgaatg gggcatttaa
    27721 atccacgtcg attacttggc ggaagtctgc cgcatccatt tcgatcatcg gtgtccgttt
    27781 gatgatccct gcattgttga ctagaatatc aactgaacct acatcttctt tgatctgttg
    27841 aaccatcgct tgaaccgctt cttcatcggt cacatcgcag acataacctt ttgcatcgat
    27901 gcctgcttct ttataatgcg ctataccttc atcaactgat tcttgattca agttattaaa
    27961 tacgattttt gctcctgctg tagctaaaga gcgtgcaatc tcaaatccaa taccatagac
    28021 agcaccggtg actaatgcta cttttccatc taaacgaaac atatccatac taaattccat
    28081 cattttctct cccttatgct tattttaatt gatccatttg caccatatcc atgtctgtat
    28141 aggtgatatt ttctccgcac attgcccaga tgaatgagta attgcttgta cctacacctg
    28201 aatggatcga ccagcttggc gaaataatgg cttgttcatt gtctacaact aaatgttttg
    28261 tttcatctgg tttgcccatc atgtggaaca cacgtgtatc ttcattggca taatcaaagt
    28321 agacataagc ttccatacga cgttcatgtg tatgacaagg catcgtattc caagcgcttc
    28381 ctggttctaa aatcgtataa cccatttgta attgacagct ttcgcataca tttggatgga
    28441 tgtattggta gattttacgt tcattcagtg tcaggccttc accagtctcc attggtttta
    28501 cttggtcgat actgattttt acatttggat atttatgatg agctggaaca gaactaatat
    28561 agaatttagc tgggttttca gcatcttgag aagaaaatac aacatgtcgt gtttcttttc
    28621 cgatatagta tccgtcttgt tttttcattt cttctttttg cccatcgatc tcgatgaatc
    28681 ctggtccacc gatattgatc acacctagtt cacgacgctc taagaagtaa tcaacaccta
    28741 gttctttgtc taatgtgatt tctaattctt tttcagtagg tgtcactcca ccaaagatca
    28801 ttcgatcgtt gtgcgtataa gtcaaactga ttttccctgg ttcaaatacc ttttctacta
    28861 aaaattcttt tctcaattgt tccgttgaat aatgacggat atcttctgga ctgtgtgtat
    28921 aacgtgtttc catttcttgc attttcttct actccttcta tcgaatgatt tttccagagc
    28981 cgcatgctaa aaattgtgcg acttcttctt gactaaattg gttgcagtcc ccatggatcg
    29041 tatgcttcaa ggcagaagct gccgtagcaa aatcaatgat ttcttgcgga gacatattgg
    29101 ataaaatccc atggagcacg ccacctgcaa aagcatctcc gccacctact cgatccacga
    29161 ttggggtgat tctatggact tgggattcca catattcatg gttcatccac aatgtcccct
    29221 tcaattcatt ttcacttgct gaatgaacgg tacgcttggt cgaataaaac acctgaatat
    29281 tcgggaactt ttcctgcatt ttttgataat aataaattag aggttgttct tctgcttctg
    29341 gcgctttctc gatacctaac aaataaagag catccatttt tccagctgag cagatatcta
    29401 ctaaaggtaa aagctgatgg attacttcgc tggcttcttg ctgtgtccat aattttccac
    29461 gatagttgat atcgaagctg atcaggcagc cagctttttt tgcagcttct atcacctgtt
    29521 tcgtcatttt ttgccatttt ttagacaaag ctggggtgat tcctgaaaca tgaaatatat
    29581 ctattccttg gaataactcc tctagcgacc attccaaatg atccatttca gcaaaacttg
    29641 atccggcacg atcatagatc actgaggcgg cgcgttcgcc actaccagtt tccatataat
    29701 aagtacctag tcttgatcca ccagataata cctgtttcgt atctactcca taacattgca
    29761 agtgcttttt gactgctttt cccaatgcgt tatcaggtac ttttgtagca aaaatcgctt
    29821 catgtccgta attagctaaa gaaattgcta cgtttgcttc ccctccgcca taatgggcag
    29881 cgaatcgttc acttgaatcg atacgggttc cctgctctgt ggacaatcgc aacatgattt
    29941 ctccgagtgt cactactttt cccataagca gctacctctt gatttctttc atttttgctg
    30001 catactgttg cgcgacttcc gttactttgt caaaatctcc tgtagctgcg ggtgctaaaa
    30061 ggttcccacc tactccgact gttacgacac cagcatcgaa ccactctttc atattgtcca
    30121 gactcacacc gccagttggc atgatattga cttgaggcat cggcgccttg aaggcagaaa
    30181 tgatacttgg tccataagcg cttccaggaa atagtttgac gatatctacc ccgcttttca
    30241 gcgctgtttt gatctcggta atagtcatac atcctggtaa gtaaggaatt tgatacagat
    30301 tgcacatctc tgccgtttct tgatcaaagg aagggctaac aatatactct gcgccagcta
    30361 agatagccaa gcgagctgtg acagcgtcta atactgtgcc tgcgccaatc actatttctg
    30421 gttggtcttt gtagaatgac aaaagttctt tgattacttg atctgcttga gggactgtaa
    30481 aagtcaactc aataccagtt agaccacctt tgatgattgc gtggcaagca ttcaaagctt
    30541 cttctttgct ttttccgcgt accaccgcaa tcactccggc gtttttcaat cgagataaaa
    30601 tatcaatttt tttcatcttc tccacctatt tcctatttag aaattttatt ttataacttg
    30661 gaaatctata cccatattat acggcagaaa agaaaaattg caaacgcttt tttcattatt
    30721 tttgaattct ttgctcgatt tcgctttttg tttgcttgag ggcactgatt atttgttctt
    30781 ttgtctcatc tgttaaacga tatttcggca tacttacact aaatgcacca atgatttgtt
    30841 cgtttttcac gattgaggct cctatacaaa aaatatcacg ttcgatttct tcatcgtcaa
    30901 atgcgatgtg ggtttctttt accgttttga tttccttttt caaccgtaat gcattggtga
    30961 tcgtatattc tgtgtaaggg atcaacggat gcttttcaag ataatcagtg tactgctctt
    31021 cactgaattc tgctaatacg gctttgccca tcgcagaatt gtataatggt cgggtgattc
    31081 cgatttttga agacatgcgg atcgtttgat gttttggatc tagtttgttc acgtacaaga
    31141 tctcattgtt gtccaatacc cctaagtgga tagtctcatc gatcttttgc tgcagctgtt
    31201 ctaaaaacgg taaagtcaac tctaccaaat cgatttgttc aatgttttta ttcgcgtagc
    31261 gaataagctt tgcacctaaa cgatagtttt tctctgaatt tttatttacg tagccgatca
    31321 tcaacaaagt atctagtatt ttcaagactg tagaagaggt cattcccaca ccctttgcga
    31381 tcgtctgcaa agagacatct tggttttccg ctaaatagtc tagtatttta gaggctttga
    31441 tcaatacagt tccataaggc tttttatttt ccatataata ttctcctatt tctttataag
    31501 aaaaattata gcggaaatac catcaattta aaagagatat ccagatataa actaccattt
    31561 ctctttacag gcaatttaat gttgaagatc cgcttcgttt ttacatcaaa ctttgatata
    31621 aagcaatgat ttcttctact gtcgtttctc ttggatttcc tggtgtgcat acatctcgca
    31681 aagcatcttc tgcaattgct ggaatatctg cttcttttac acctaattca gaaatggttt
    31741 ctgggattcc aaccgctttg ctcaaccgat cgatctcacc acaagccgct tctcttacat
    31801 cttctaaaga catttcagct gctcctttaa tccctagaac aagagcgatc tcacggtact
    31861 tttctccagt atattctttg ttatatttca tgacagttgg caaaagtgct gcacacgcta
    31921 cgccatgagg aatgttgtac catgcactta acgggtgagc catcccgtgt accagtccta
    31981 aaccgacatt tgagaagccc attcccgcaa tatactgtcc taatgccatt ttttctcgcc
    32041 cattttgatc gccatctaca gaagcttcca aagaatggcc gatgatttcg attgctttta
    32101 aatgcagcat atcagtcatt tcccaagcgc cttttgtgat aaacccttca atagcatgcg
    32161 tcatagcatc catacctgtt gatgcagcca attttttcgg cattcccatc atcatatcgc
    32221 tgtcgatgaa cgcgacgatt gggatatcat gaggatcaac acaaacaaat tttcggtgat
    32281 ttacttcatc tgtgatgacg tagttgatcg ttacttctgc tgctgttcca gaagtcgttg
    32341 gtatagctaa tataggtaaa catggatttt tcgtatcagc aacaccttct aaactaatga
    32401 cgtctgaaaa ctctggattc gtcaaaataa tcccaatggc ttttgccgta tcgattggcg
    32461 aacctccacc gattgcgatc aaacagtctg cctctgcctc tttaacaaaa gccactccat
    32521 cttttacatt ttggatcgtt ggattaggta cgatcccgtc gtataaagca taagcgattg
    32581 ctgctttatc tagaagttct gttacttttg ttgctatacc gtgctcgatc aatcctttgt
    32641 cagtaatgac agctgctttg ccgaatcctc gctgctgaaa ttcagggacg atcgactcaa
    32701 tcgctccttt tccaaaatac gatgtttcat ttaaaatcat tcgattactc attttctatt
    32761 cctcctaatt tgtattattt ttcacaataa aaacgaaaga accccaagca accggttgcc
    32821 cagttgcttg ttgctacagt tcaaagactg gcatcaattc ccaactgact ggagaggcat
    32881 ctggatttgt ctccatgata tcttccatgt acttccacca tttttgattg atagatgtat
    32941 ttgccatttt ttcccataac ttttcatctt cgatcactaa gtaaccaaat aacaaacttg
    33001 tactcggatc aagatagatc gaatagtcga tgaccccgtg ttcacgaagt gctgtacgca
    33061 tttctggcca aagttcatca tgtctttttt tgtattcttc ttgtttttct gggtaaactt
    33121 tcatgcaaaa agcttttttc accatgtcag acacctgctt tcgtatgaac ttcctccaaa
    33181 taacctgctt ttggagtgac accaaacgca tctgctaaat gccacaggtt ttcgtccgtg
    33241 attgtttgca agattggtcc ttgcgctttg acgtacgtat agacttctgc tgccttttct
    33301 gctgtctcga tcaaaccaaa gacttcatcc atgtcttttc ctgacccgta gattccatgt
    33361 tgtggccata aaaccaatcg agtttctttc attttttcag ctgttgcttc cccaatctca
    33421 tttgttcccg gaaccatcca cggaatgatg gaaattcctt ctggaaaaac aactaagcat
    33481 tccgtacaca tttgccatag cgtacgacta aaggaacgtt cgtctaattc atgcgtaaaa
    33541 ctcatcgcta atagatgact agcatggcaa tgcataacga tccgattatc tgggtctact
    33601 tctaaacggg cgatatgact catgaaatga cttggcaact cgcttgtagg aaggcattcg
    33661 ttttccagcc cccacaaaag ttccagtgtt ttgccatctt cagcaactcg aacgattcca
    33721 agattctcgg caggttcgct cgctacattt ttaaaatatc ttcctgaacc agttacaata
    33781 aaatagcatc ctgctaaacg gctggcatca aaaatcatcg agatgcggcg gatcactttt
    33841 tcaggatcca agaacggtgt gatctcttct tcttttagta aataagaaat attcccgcca
    33901 tttctttcat cccatcccaa gcgataaaga ttcgacgtcg tttcgatcat ttctttcaca
    33961 taaggcgctt gtaaaatatc tatttttttc aatatagcca cactcctatt ttcttaattc
    34021 caacacgtct ttttgatatt cctggacttc ttccagccaa tccaaaccaa ctggaacttg
    34081 atttttgtaa cagaaataat tccatacatc ggcatacggg aaatctttca attcttccgt
    34141 ataagccatt cttgaagtaa agtcaaaagc cagctccatt tctttcaatc gctcaactgg
    34201 ttccagcata gctttcagca gtgctttttg tgtatttctc gtaccgatga cccaagcagc
    34261 gatacggttg atcgttgcat caaagaaatc cagaccgata tttgtttttt ccaacaattc
    34321 gttacggact aattctttag cgatttcctg caattcatca tccatcaaaa ccacatggtc
    34381 tgaatcccaa cgcactggac gggaaacatg caacatgatt cctttgctga ataaagctaa
    34441 tgaagataat ttattggaaa tcacttctgt tggatgaaaa tgtccagcat ctagacaaac
    34501 aagtttgtca cgagtcaacc cgtaacccat ataaaattca tgggaaccga ctgtataagc
    34561 ttcagcgcct aaaccaaata gtttgctttc tactgcatct tgtacatacc gttcatctaa
    34621 ctcttctgta aaaatttcat cgagtgattc cattaaacgt tcccgtgggg taaaacgatc
    34681 aatcggatta tctttcatcc catcaggtac ccaaaagtta ttgatacttg tttgcccagt
    34741 ttctttccca aagtactccg cgattttgcg gcttcttttc ccgtgttcga tccagtattc
    34801 acgaacttct ttatctggat gagccaatgt aaagccgtct ttcatcattg gatgcgaaaa
    34861 gaaagtcgga ttgaagtcaa gaccgatttc ttcttttttt gcccactcta cccatttttc
    34921 aaaatgtttc ggctcaattt catttagatc gacagattct tctgtatcca aatagatagc
    34981 atgaaggttc aatttgtgtt tgcccggtat caacgaataa gccttctcca gatcttgtct
    35041 aagctcttca ggtgttcgtg cggctcctgg aaaattccct gtcgccatga ttccaccggt
    35101 caattcgcca tccggtgtca agaaaccttt gacatcatct ccttgccagc aatgcataga
    35161 aattttgatg tcctgtatct tttctagtac agcatctgta tctacaccga tagttgcata
    35221 tttttctttt gcttgttcgt aatttctttc aatcgtgttc atacgattac tcctttgatt
    35281 ttattcacgt accattccat cgtgttttgc ttattttcat ctgggtaaaa agtttcacat
    35341 ggaaacgact gtttgattac atttcttgct tgttctatat tcgaaaactg acctgtactg
    35401 agcatttgca tggcaacatt cccgattgct gtggcttcac taggtcctgc ttctacaatg
    35461 attccggcat aatctgctgt caattgattc agtagacgat tattcgatcc gccccctacg
    35521 atgtgcagct tttccaattt cgttccagtt aagtgttcta ctttgatcag ctcttgtgcg
    35581 taacaaagag caagattatc atacacacaa cgagcgatag ctcccgatga gacagggact
    35641 ggctgctctg tttcaaaaca gtattgctga atctcttcta tcatatttgg gggattcaaa
    35701 aatcgagggt cattgatttc catcacttgc tgcagcggtt tttcttttgc agcaagctct
    35761 gccaattccg cataactgta ctcgtactct tgcattcggg caacttcctg gatcaaccac
    35821 atccccatga tgtttttcaa aaaacgcacc gtgttatggg caccccattc attcgtataa
    35881 ttttcagaaa aagcattgcg agaaacattg gcgatctttg tttcaatccc taaaagcgac
    35941 catgtcccac tgctgatata accccaacgt tctccttgac ctggcgttcc taatacagct
    36001 gacgccgtat catgacttgc tactgtgata aaggtcacat ctggcaaatc atattttgaa
    36061 aattgttctt tttgcagatc tcctagtatc gttcccggct ctgtcaatgg tggaaataat
    36121 tccttcgaga caccgatttt ttcaagaagt tctatttccc attcttttgt ccctgcattc
    36181 agcagctgca tagtagatgc attagttttc tccgtcacca tgttaccagt aaagacatac
    36241 cctaaataat caggtatcag taacaattta tgtgcttttg ccaaattttc tttttcttca
    36301 gcaaacaatt ggaacaaggt attgaaaggc tggatctgaa tccctgtctg ctcatacaac
    36361 ttatctagag aatagatttc agaaaactgt tctactgctc cattggtacg cttgtcccga
    36421 taagcaatcg gatcatttat ccgatcccct tgttcatcaa tcagacagta atcgactgcc
    36481 catgtatcga tccccgcaaa aaggcgatca atccctgtct gctttaattt ttcaaatcca
    36541 atcaaaatct ctttgataag ataatcgatc ttccagcgat cgtgaccgtc tttgttccga
    36601 aagccgtttt tgaaccggtg tacttcttcc aagatcaaag aaccttttgc atccctcgta
    36661 cttttcatta gtcgtccgct tgatgcaccg atatcgatgg caacaaatgt tggattcatt
    36721 tctccacctc ttttacttta ttgttgatta gcactttatt aataaagcgt tatcatttat
    36781 tgacaatgct atcataatac agaatcttat attttgcttc acactatttt ggtatataat
    36841 tgcattatct tgacaagttg ggagtgttta catggattac tgggaaaaag ccaaccactc
    36901 atggtcggaa gactcaaaaa gagtgatctt gacgcctaca caaagaagca ggaatttatt
    36961 tttttatatc caagaaatcg gttacttcaa agcaccaatc ccttactata ctgaacgagc
    37021 acatttacct tcttacttga taaagtttac tttgtctggc aaaggttcgc ttacatataa
    37081 tgataatcag caacagctaa aagctggaga cttatttttt attgactgcc aagaatatca
    37141 gcgttatcaa acgatcagcg aagaaccatg ggaaatggat tggatccatt tgaatggtca
    37201 gaacgtccaa tcattttatc aggagttcac aaaagacggc cataacacct tccattcaac
    37261 aggagatcca tcagaaaaca agatccacca tttgatcaca gaattgatcc acatgcaaca
    37321 aggcacgaat gcccgtaccg atttccagtc ctctgtagct atccacgagt tgctgaatga
    37381 actgattttg caaaagtatc agctagattt cgaagaagag gatattcccg cctacgttta
    37441 caaactgaag caatacttag acgaacattt caaagaaacg attacacttg atcaattaga
    37501 acaacaattc ttattgaata aatatcagct gagcaaagat ttttccaaat ttatcggttc
    37561 tccaccaatc gattatttga tcagcaaaaa actttcttac gctaaagacc ttcttcggta
    37621 tacagaattt acggttcgaa tgatttccga agaaataggg attgagaatt ttgcttactt
    37681 tagccgcctg ttcaaagcaa aaacaggact atcgccgaga gactttcgta aattcagctg
    37741 attcatataa tatgtacgtc attttttcaa aatattttga aatatataga taaaaataa
//
LOCUS       DASNNU010000018        37068 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.018, whole genome shotgun sequence.
ACCESSION   DASNNU010000018 DASNNU010000000
VERSION     DASNNU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.018"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..386)
                     /locus_tag="U8116_000864"
     CDS             complement(<1..386)
                     /locus_tag="U8116_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433876.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKIL"
     gene            complement(571..969)
                     /locus_tag="U8116_000865"
     CDS             complement(571..969)
                     /locus_tag="U8116_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEN8433877.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(966..1739)
                     /locus_tag="U8116_000866"
     CDS             complement(966..1739)
                     /locus_tag="U8116_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN8433878.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1736..2419)
                     /locus_tag="U8116_000867"
     CDS             complement(1736..2419)
                     /locus_tag="U8116_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN8433879.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2586..3473)
                     /locus_tag="U8116_000868"
     CDS             complement(2586..3473)
                     /locus_tag="U8116_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN8433880.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3470..4339)
                     /locus_tag="U8116_000869"
     CDS             complement(3470..4339)
                     /locus_tag="U8116_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN8433881.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4476..5762)
                     /locus_tag="U8116_000870"
     CDS             complement(4476..5762)
                     /locus_tag="U8116_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN8433882.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6035..6613
                     /locus_tag="U8116_000871"
     CDS             6035..6613
                     /locus_tag="U8116_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN8433883.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6613..7542
                     /locus_tag="U8116_000872"
     CDS             6613..7542
                     /locus_tag="U8116_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8433884.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7539..9275
                     /locus_tag="U8116_000873"
     CDS             7539..9275
                     /locus_tag="U8116_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN8433885.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9338..10174)
                     /gene="pheA"
                     /locus_tag="U8116_000874"
     CDS             complement(9338..10174)
                     /gene="pheA"
                     /locus_tag="U8116_000874"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEN8433886.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10171..10680)
                     /locus_tag="U8116_000875"
     CDS             complement(10171..10680)
                     /locus_tag="U8116_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEN8433887.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10673..11968)
                     /gene="aroA"
                     /locus_tag="U8116_000876"
     CDS             complement(10673..11968)
                     /gene="aroA"
                     /locus_tag="U8116_000876"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN8433888.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11981..13072)
                     /locus_tag="U8116_000877"
     CDS             complement(11981..13072)
                     /locus_tag="U8116_000877"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEN8433889.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13089..14255)
                     /gene="aroC"
                     /locus_tag="U8116_000878"
     CDS             complement(13089..14255)
                     /gene="aroC"
                     /locus_tag="U8116_000878"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEN8433890.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14257..15321)
                     /gene="aroB"
                     /locus_tag="U8116_000879"
     CDS             complement(14257..15321)
                     /gene="aroB"
                     /locus_tag="U8116_000879"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEN8433891.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15354..16376)
                     /gene="aroF"
                     /locus_tag="U8116_000880"
     CDS             complement(15354..16376)
                     /gene="aroF"
                     /locus_tag="U8116_000880"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEN8433892.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(17035..18009)
                     /locus_tag="U8116_000881"
     CDS             complement(17035..18009)
                     /locus_tag="U8116_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEN8433893.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17990..19873)
                     /locus_tag="U8116_000882"
     CDS             complement(17990..19873)
                     /locus_tag="U8116_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEN8433894.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19891..22116)
                     /locus_tag="U8116_000883"
     CDS             complement(19891..22116)
                     /locus_tag="U8116_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN8433895.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22231..23265
                     /locus_tag="U8116_000884"
     CDS             22231..23265
                     /locus_tag="U8116_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN8433896.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23332..24345)
                     /locus_tag="U8116_000885"
     CDS             complement(23332..24345)
                     /locus_tag="U8116_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN8433897.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24423..24611)
                     /locus_tag="U8116_000886"
     CDS             complement(24423..24611)
                     /locus_tag="U8116_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEN8433898.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(25051..25179)
                     /locus_tag="U8116_000887"
     CDS             complement(25051..25179)
                     /locus_tag="U8116_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN8433899.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(25182..26555)
                     /gene="rlmD"
                     /locus_tag="U8116_000888"
     CDS             complement(25182..26555)
                     /gene="rlmD"
                     /locus_tag="U8116_000888"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN8433900.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26666..27721)
                     /locus_tag="U8116_000889"
     CDS             complement(26666..27721)
                     /locus_tag="U8116_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN8433901.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27731..29161)
                     /gene="gatB"
                     /locus_tag="U8116_000890"
     CDS             complement(27731..29161)
                     /gene="gatB"
                     /locus_tag="U8116_000890"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEN8433902.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(29158..30630)
                     /gene="gatA"
                     /locus_tag="U8116_000891"
     CDS             complement(29158..30630)
                     /gene="gatA"
                     /locus_tag="U8116_000891"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEN8433903.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30630..30935)
                     /gene="gatC"
                     /locus_tag="U8116_000892"
     CDS             complement(30630..30935)
                     /gene="gatC"
                     /locus_tag="U8116_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEN8433904.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(31041..33077)
                     /gene="ligA"
                     /locus_tag="U8116_000893"
     CDS             complement(31041..33077)
                     /gene="ligA"
                     /locus_tag="U8116_000893"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEN8433905.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(33094..35328)
                     /gene="pcrA"
                     /locus_tag="U8116_000894"
     CDS             complement(33094..35328)
                     /gene="pcrA"
                     /locus_tag="U8116_000894"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEN8433906.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35483..37042
                     /locus_tag="U8116_000895"
     CDS             35483..37042
                     /locus_tag="U8116_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEN8433907.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT        10236 a         8050 c         6688 g        12094 t
ORIGIN      
        1 agaatctttt gctgacgtct aaaaatagaa aaagaatttt cgtcatctat ttgataacgc
       61 atttgttttt gcagttctgc tgcagtcaga tggatcttcc catcgtttgt atcgatcatt
      121 aaaccttctg gaaacagttc aaccactgct cgatcccaag attcaagaga aagctcgata
      181 taatctttta ctggtaattc caattctacc ttaaaaaagt gcaaaacagc cgacagccct
      241 tctgcttgat agatttctcc taatgttagc ccctctagat ggaactctgg tgaaaaatgc
      301 agcaatgttt tattttcctg caattgaaca tatagaaatg tagatcgctc aatcagagta
      361 tcttttaaaa caactaaaaa attcattttt ctcccccttt tttcatcatt tttaaaatag
      421 catattttga aaagaaagtc tgatgcttag agtagaattt cataaaaatt tactatttca
      481 taagtattca cgagaaaaat acaaacaaaa aagcggaaat aacgctcatc cgccttcttg
      541 tttgtgttta tttcctcttc aaacgcttgt ctatgattca gacttttctc tatcgttatt
      601 ttcatctaaa agttcttgca ttccgataat ttctcgatat tgatgaatct tagtgatacc
      661 aatgactttg tctacatcaa tactaaaaca aataccatta cctggaatat cgatgttgat
      721 gccttgacca atcgcatcca ttactttatt cgtcaaacta tcgggtacta atgtcaacac
      781 aatatctttt tctggcgaaa tgtccaaacc taaaaatttc cctgaatcat gtacaccaga
      841 acctcttcca tgtaagatcg ttccgcctgc tgcacctgct tcttttgaaa agtcgataac
      901 atcttcgccc attcctccgt caacgatcgt cacaatcatg tgaagattca atgctacaag
      961 atctttcatt tgctttcccc caatttttgc aatctttctt tttcgattct gtctttttca
     1021 tcttttcgtt tataaatata tcctagtact aaagtggata atattggtgt caatgcaatc
     1081 attgcaacca caccaaaccc gtcaatgatc ggatctcttc cttcaatttg tgcagcgatt
     1141 gccacgctca ttgataaaat aaatgtcgaa cacatcgggc ccgttgctac tccaccgtta
     1201 tcaaaagcca ttgctaaaaa gatcttgtcc acttttcgtc ctaaaataaa tgctagcccg
     1261 tatcctggga tcaagaaata ataaagagag aatccgacga gcaaacgcca catagacaat
     1321 cctactgctg cgccgatccc aattgaaatt accatcagca tgattttcga tcggattctc
     1381 ccctcgctca attcttcaac ctgtttgatc attacatgaa tcgccggctc agcaaaccct
     1441 actaagaacc ccattaaaaa gccaagggga accagtatat aattgtaagg caatcccgca
     1501 atagcactgc ctaaatgctg tcctaccggt acataagcca tattgactcc gtgcaggaaa
     1561 aggatcaacc caatagtcgt tatcaagaaa cctttcataa tgtcaaagaa acgctttttg
     1621 cgcatacgaa aactaatgat attcattaaa acaaatagga ttacgatagg tgccactgcc
     1681 aaaatgactt ccatggtaac gatttcgatt ccatctatca cttgctgcca gaaactcatt
     1741 ggaagatcac ccccaaaatc atcacggcaa gaatcggccc taacgaagca ataccgacca
     1801 ttccaaaact gtcattttca tttttctttt gtttgatcat actagtcatc cctccggcaa
     1861 gggccaagat aaatggaaca gttactggac cagtagtcac tccccccgaa tcaaaagcaa
     1921 tcggcataaa ttcggaactg gtgaaaaatg aggcgatcaa aacgcctatg tagcctatag
     1981 ccatcaattg ataataggat agcttgaata cgacccgaag cagcgcaaag gctagaaata
     2041 ctccagtgcc gacgctgacg atcccaatca gcaacactct gccaatctct ccgtcagata
     2101 tatcatatac ttgttgtccc aaaacttgca cggaaggttc agcaatcgtg atcacgatcc
     2161 cgatagcaaa tcccaaactg accaaaacgg tcaaactttt ctttttgatc atatatttcc
     2221 ctactagatc tccgacttcc atcatagaat aatctgctcc aaacaaaaag agcgtcattc
     2281 cgatcatcat gattgccgca ccaaccagaa atgaagcgag gtcttctcct tctaacggtg
     2341 caaaaacaaa cgttaaaatg acaatcagga cagtcatcgg caatatagca gcaatcactt
     2401 ctttaaaatt ttttagcatg tatcctcctc ctttactctt taagtttacc ttaaaatgaa
     2461 agagttttcg agcacaaact taaaacgtta tcatttaatt tttctcaaca taaaataaca
     2521 cacgtcccaa aagtatcttt ttacttggaa aaatgacttt gaaggaacgc gcttattgtg
     2581 tttttttatt ttacagagcc gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat
     2641 acgatggcag ttggtaaagt agcgatgact aatccagcca tgatcattcc atagtctggt
     2701 gtataggaag cacccatagc agataaaaca agcggaaccg tccgtttatc aggagattgc
     2761 aaagctacta agggccataa ataattgttc cagctactca tgaatgtgat gattgcagcc
     2821 gcggcatagg tatttttagc cgtcggcata tacactctaa aaaaaatact tagttctttt
     2881 agaccgtcta atcgagcggc ttctactaga tcttttggaa aagatttaac attttgtcga
     2941 aagaagaaga tcaaaaaagc agtgctaaca gatggcagga tgacaaccaa gaaactgttg
     3001 attcccaaaa ccgttccgtt caattgtgaa aacattcgat ataaagggat catcaatgcc
     3061 gcaaaaggaa ccatcatcga tagcagtaga atattgaaaa ttaggtcttt tcgtttactt
     3121 tgataaatct caaatccata tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc
     3181 accaaagcga tcatcgctga attccaaagg gaacgagtaa aattcagctc attggaaaac
     3241 aaattttgaa aattgatgat caagttactg ccaatcttta tttttcccga tgtgatatcc
     3301 acgggtgtat tcgtcattcc caaaaccatc cagacaaaag ggaaaatgga aataaatgat
     3361 aggatgctta atgctgtata ctttaatact gacccaattg tcgaacgaag ttctaatgct
     3421 tgttttttga cgttggggcg ttcgattgtt tctttttcta ccatattttt catcgacttt
     3481 ctcctcctac tttcgtttgg attatcgaca atatcacaat aaaggaaaca ataacaaagg
     3541 aaactgctgc cgcatatcca aaattaggcg tgaatttata actcaaatta tagatgtatt
     3601 gagatagggt ggtcgttgca tttgctggtc ccccgccagt aatattttgt acttcatcaa
     3661 aaagctgcaa tgtcccgatc gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat
     3721 ttgggatcgt cactttgata aactgctgcc atttagaagc accgtcaata gatgctgctt
     3781 cataaatctc cgaatcaata ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag
     3841 tccaacgcca agtgatcgaa agaatgatca gaactctcgc ccaaattgga tgagtcaacc
     3901 agccaatcgg tgtgtcaatc ataccaatgc ccaacaacaa gttgtttaca aggccagatt
     3961 gagaaaatag acttttcatg atcagagaat aactgaccat cgaagtgata catggaagaa
     4021 agatagcggt tcgaaacaac cctttatatt tcaacttttt atcatttaaa aggttggaaa
     4081 taaaaagtgc tagaaacaac atgatcggta cttgtacaag taaatacagg aatgtattaa
     4141 aaaaagcttt tttaaaagtt gcatctgtca acatccgctg ataattacct aacccgttaa
     4201 atgtcatatt gacaggggga ccactttgaa aagacgtgat caaagccaaa accatcggaa
     4261 taaaaaccat gattgatatc aaaatgactg gcaagattaa aaaagcatgc ccgtttttga
     4321 ataaatcttt ctgcttcatt cttttccctc caattctatc attgattatc tacatgaaaa
     4381 gacaagaaag acaatatctg taaggcagac gtgatctttt ttgtcttttc gtttgtctga
     4441 tgaaaaagcg aagaaattca cttttttacc accgatcaat tagccaattg tgcttctact
     4501 tgtttttgtg tatctgctag taccttgtct gtctcttctc cattgatgat tcgatgaaga
     4561 gcttcagcta cgactgcttc aattgcatag gtctcatgtc cgtaattcac ttctggtatt
     4621 tcagatgtcc atgttgaaaa atcgtcaaag atcttttgac cactgtaaaa ttcagaagct
     4681 tcttgataag cttcttgttc attcgcgcta agcatcgtag atactagtcc gatttctttc
     4741 gcaagagtgc ccatcagttc ttcattcgaa gcaaacgttg cttctaggaa atctttcgct
     4801 gaatcttctc cagctactcc tttgatcaca taccagccag cgccgccaag gttagaagct
     4861 tgcgcttttt gcagattttc tggaagtgca ggtattggtg caatcttcca ttttccagat
     4921 tggttttctg ctccttggat cgttgaagaa taccaagcgc cagttgggct agaagctact
     4981 gctcctgatt gtatcgcagt tactccagca ttccagtcag aggtttgttc gactagatct
     5041 tctttcaaaa gagtggcaaa caattcaaga ccatacttta aagatgcatt gtcttggatc
     5101 gtgactgtct ccccatcttc agatgtatac cattctcctg cctgttgcat aatcatcctt
     5161 actcgaccaa ggtcagaagg attgacttca gcgattttct ttcccgtctt ttctttgaca
     5221 tctcgcgcta cttgaatata ctcgtcccac ttcagattct ccatatcctc ttctgtataa
     5281 cctgcttctg ccatcagatc cgtacgatag aagtttgctg ttacgcccga atcgaaagga
     5341 acaccgtata ttttatctcc tactttgttt accgcaaatt tatacggtgc aaaatgatct
     5401 tcctttacaa tagcagacaa attagcgaat gcatctggat aagaagtcaa atagccttgt
     5461 atcctataat cttcaatcaa gacgatattg ggcaatcctt ctgtattccc gctcgctaaa
     5521 gcggtgttca gtttctgaac gatatcatcc tgagacatcg taacaacttc tatctctgtt
     5581 tcttcgttct cataaacttt ttttgcttca ttcactgctt tgatattgaa tgtctcatcc
     5641 cacgcccaga cagtgacttt ttcttctttg tctgctgtac tactagcact tccgcatcct
     5701 gttaagaaac ctgatgctgc cgctaaacaa acgactccag caattaactt tctgatgttc
     5761 atttttatcc gctccttttc catccattag ttgtttacgt ttgctacatt catcatacca
     5821 aaatttgaaa acggtttctt tattattcag atacttcaaa aatttctcct aaaataataa
     5881 aaaaatacaa atcaactatg acaaaaggaa taatcttagc atctagacca aagatcgaaa
     5941 tggtgaggtt tctttaaaat aaaaatatcc ttgcattttc tcctagaaat aagtattttt
     6001 aaattacaag actattctta aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga
     6061 tgatgaacag acgattttag atggaatgaa acagttgatc aattggtctg agcacgggtt
     6121 tcaattaata gggacttttt ccagtccgct gaaagcgctt gatttttctt tggtacatgc
     6181 aatcgatatc gtggtcacag atgtaatgat gcctgaatta aatggaatcg aattatcgcg
     6241 gctattaaaa cagcaacaac ctgaaacaca gatccttatc ctctctagct atgatgaatt
     6301 tgatatggtt aaagattctt ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt
     6361 gagtcctgat ttttttctaa attccttaac tgccgtttca caaaaaatca aaaaaaagcc
     6421 caacaaaagc ccaatcacca atcgttatga gcagatctgc gagaatcttt cacagcagat
     6481 tgctggacaa actcgtccat tgcttgaccc tcttcttttt gaacacacct tgtttttctg
     6541 ttatataccg aaaaacggcc attcagttca aaacaaggct ctaaacaaaa acagtcgatc
     6601 acccattcat tgatggatag cgacgattcg tttactattt atcctgtttc taccttttcg
     6661 gaagaatccg gctatgtaat caatactgat tccaaagata cacttaggaa gtttgttcaa
     6721 aagtttcgtc aagaagtttt tccaggtgat ttttttgttg tttctgattc ttttgcaatc
     6781 caagatatca atgaaatatt ccaagagatc agaaattgca ccaaaggaca acgtttctat
     6841 cataagggaa agaaccttgc ttatcagcat gaacttcttc cgttattaac caaccacgag
     6901 cagcaagcaa gtagttattt aacaaaaatc gttcataaag actacgcttt agctgtcaaa
     6961 gaacttgaga atttcttcac gacgatattg gaacagccta ttgatccagc ttatttgaaa
     7021 catgaggcaa tcaatcgttt ctatgcatta ctcaacgctt tggctgacga gcatgttggg
     7081 aatgaagaac tttctttgct caagatcgcc cttccttctt tattagccga tgctgagtat
     7141 ttcgaagatt tttctattct tgtcaaagaa acacttgaga aactcaccca cttttctgca
     7201 aatgcaaaaa aagacaattt cgatatcttg actttgatct acgagttcgt ccaggaaaat
     7261 tatgagaaag aaatcaattt acaactttta tccgaaaaat atcatttttc atatagctat
     7321 ctctcagcca tctttactga aaaatttggt atcaactttt caaaatatct aaaaaaagtc
     7381 cgtatcagta aagccaagct ctttttgacg gaaacacggc ttactttgac ggaaatctgt
     7441 gagaaagtcg gctataccga actgggttat ttctccagag tttttaaaga agagacagga
     7501 atgacacctt ctcaataccg taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc
     7561 atgaactctt tgccaaactt ctgttcattt tgttcatcgg tctttttctg caagccatcg
     7621 tcatttcttt gtttatttat catcgatcaa gggatgccta tatccagctg ttcaatcaat
     7681 cgaatgatgt ggtactaaaa aagattcaat ctgaatttga aagtttgaat gatacaatcg
     7741 agaatacatt agccgctttt gattctaatc cagctgtcaa aagctatttt tccaatgatc
     7801 cagcccaaca tatggaacag cttcagcaac taagaacaat ccaaaaaatg aatgactctc
     7861 tttcaaaaat ccatccaatg atcgattacg atgtattgat ctttggagaa aatggaagaa
     7921 cttttgtcgg aaacgacatg ctcactgccg tatctgccga ttcttttttt caatctgcga
     7981 ttgctcagag agtgaatgaa cgtgctgctg atacacaaat gctttttgca caccacggat
     8041 tgactcttcg cgataagaaa gcacccagcg tcttctttgt caaaaaactg aaaaatacat
     8101 tgaatcacgt ttacggatat gctgttttat cgatctcatc caagcaacta gcgaatcttt
     8161 ttcaatctgt agtaaatcct gagatatcga agatctcctt catgaacgaa gaacagacga
     8221 tcatcgcatc aaatgaagaa aatacaattg ggagccgatc aatgattttc aaaaaactaa
     8281 cagtaggtga aacaacgatt tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc
     8341 aggattgtac attactcagt caagtcgatc ttcattcttt atctaaccaa atgggcgtga
     8401 tttttccgat tattttgtat aatatcgtga cacttgcttt tgctggctca ttggtattta
     8461 tgtatttgga tcgacacaca aaatcgatct accgcttgat tgattcgcta aaacggctag
     8521 aggaagatgg cttaaaagct actgttcctg tacaaggaag ccatgaagtc caattgctag
     8581 ggaatacgat caatcaattg ctaagtaatg ctcagcatca ttatgaacag acgatccaag
     8641 acgaaaagaa gaaacgttcg cttgaaatcc aaactatgca agcacagata cagccccatt
     8701 ttatttataa tacgctgact agcttgaaat ttttgatttg gcagaaaaaa acagatgaag
     8761 cagttcaagg aatcgacagt ttcattactt tactaagaag tacgattggg aaaaaagaag
     8821 aagtgattcc agtcaaggaa gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta
     8881 tgcgatatgg cgaaagaatc tcggctaaaa taatgattcc tgatgaactg cagtcattat
     8941 ctatgccgaa tatgatcctt caaccaatta tcgaaaacgc ctatcttcat gcttttcaca
     9001 ataaaacgca aggattcatc acggtgtacg gcaaaacaaa aaatcagctt cttttattcg
     9061 aagtgattga taacggcgat ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt
     9121 tttcaggaat cggcatcagc catgtagatg agcggatacg cctaatgtat ggcgatgcat
     9181 atggcttaac gatccaatca gtgattggga caggaacgac tgtaacgatt tgtctaccgc
     9241 ttcttcctaa aaatcaatca gaatatcaaa aataaagaat agccaagcgc tgattttttt
     9301 attttaagtt aaataaatca atcagtattc ttgactttca tctacgcgtc tcttctactg
     9361 tcagcactgg ataacagccc aatatttgac tttctcctcc gagcatcttg atttcttgga
     9421 gcgcattctc tactaatgtc attggacggt ccaacaacaa atcgatcacg aaaaaatatt
     9481 ctcccaagct agttttcaat ggtcgggatt caatcttact taagttgatt tcccgccagc
     9541 caaaagctgc cagcatttta tgaagcatcc ctggccgatt agcaggtagt gtcaagatga
     9601 cactcatttt tactggagct gactgctggc tggtcatctt tcggtcaccg acgatccaaa
     9661 aacgtgtctg gttcaactca ttatcttgga tattctcagc taagatctcc aatccaacat
     9721 gctgtgctgt ttctaatgaa gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat
     9781 acattgctgc agcagttgtt gattctgttg ctactaaagg aacattagga taatgcgttt
     9841 ctaaaaactg ttgtgattgt gctagtgctt gtggatgtga aaggattttt gtaatctttg
     9901 ttgctggatt gcctaaaagc tgctgcttga taggaagaac gatttcactt ttaacttcga
     9961 cttctggatg tttggaaaga agatcgattg tgtgatgaac agctccttcc aatgaattct
    10021 ctacaggaac gacagccaga tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga
    10081 tggaagcata cgcagtcaat tgttctgtag gaaatagctg acaagccgcc tgataagtga
    10141 atgaactttc tggccctaga tagctgacgt tcatatgctt ttgatccttt ctataatggt
    10201 atcgatgatc ttttctggtg tttgattggt cgtgttgatt cgatgtgtcg cactttcttc
    10261 ataccaagaa agtcgttgtg ccaataatgc ttttatttct tctcttgttt ttccaagagc
    10321 taaaggtcga atagacgttt catcttgttg gatacgatca actagacagt ccgcttctgc
    10381 ctctaagtaa agaacaatcg gctgtgtttt taaaaaacgc cgattttctg cactttggac
    10441 aatccctcca ccagtcgcga taattttgtc ttcatttgac agctttctta aaatatctga
    10501 ctccactttt cgaaagcttt tttcaccgta atagtgaaaa tattcggcaa tcgacaattg
    10561 gatatgttcc tcaatctttg tatctaaatc tacatactct ttttgtaaac attgagctaa
    10621 ccctttaccg attgttgttt ttccagcgcc catgaaacca atcagcaaaa tgctagccat
    10681 gagcttctcc tcctgacaca agttctgcta aatcgtcaaa aaatgccgga taagagattg
    10741 aaacagcttc agctccttct agttcgcaag gtccatcagc tatcaaagca gctacttgca
    10801 gcatcatacc gattcgatga tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg
    10861 gcccatggat gatcagaccg tctgctgtcg cttttatctt tgctcccatt ttctgtaatt
    10921 cttctgccac cgcatcaatt ctgttggttt cttttacttt taattcttct gcgtctttga
    10981 tgatcgtgat tccttctgct tgagtagcca ctaaagctag aatgggcaat tcatcaatca
    11041 gacgcggaat gatttctcca ccaatcgttg ctttcttcaa atgactcgtc ttgacactta
    11101 aatcagccga ttggttctgt tcattgtgat tcgtctgggt aatcgctgcg cccatctcct
    11161 ccaacacatc taagatacca gttcttgttg gattgacccc gacatttttt aacaacagtt
    11221 ggctttccgg aacaagaagt cctgctgcta gaaagaaagc agctgatgaa atatcacctg
    11281 gaacagttat ctgttgacca gttaattttt gcggtccggt cactatgatc gttttatctt
    11341 ccactgtgat ccttccgcca aattgacgaa tcatttcttc cgtatggtta cgggaacgtt
    11401 ctttttcaac gattacggat gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt
    11461 tgacttgcgc actggctaca ggcatattgt atcggatagg tgataaagag cgggttcctg
    11521 tcacagtgat tggtagatac tggtcatttt cttctccttg caagtcagcg cccatctcgc
    11581 gtaaaggttc catcactcgc cccattggac gtttagacaa cgaagcatcc ccgaacagag
    11641 tagtcgtaaa aggctgtcca gccaaaatac ccatcaataa acgtatggtc gtaccggagt
    11701 ttcccatgtc aattgattcg gttggttgga caaatgagtg gctaccgcga ccatgaatct
    11761 tgatcacctc ttcctcttct tcgatctcga cacctaattg ttggaaggca tgtaaagtac
    11821 tcagacaatc ttctgcgcgc aaaaaatttt gtatagtcgt cgttccttca gcaattgctc
    11881 caaacatgat gctgcgatgg gaaattgatt tatctgctgg tatcctaaca gtccctctta
    11941 atccatgtat ttgttgtaat aattgcattt atccagcctc ctatttgatc cagcaatgat
    12001 aactggtatt agacataatg attctttttg cttgtaacaa atcctgttgg tttttaaaac
    12061 tgatttgcag tattcctaaa atatcttccc tggtctcttg gatcttcaaa ttaatcaaag
    12121 aaactcccgc ttttccgatc agtccagtta cttctgcaat aacgccaggt tcatctggca
    12181 catcgataaa taaatcaaaa aaggcaggaa tcgcgccttg cttatggaca ggcaactggt
    12241 ctcgactttc ttttgcccga ttgaaaaaag aaaagatttc cgattggttc tcagtcatga
    12301 tccattctgc tatttgtgtc atttcttgtt gccaatctgc caatcgttcc aataatatct
    12361 gcttattgct caacaaaata tctgtccaca tgacaggatc tgaggaagcg atcctagtga
    12421 tatctcgaaa gcctcctgct gccagttgtt tggctcgcgg atactcttga ttgaattgtt
    12481 ctgcctgatt gactaagcca gctgccagaa tatgtggaaa atgacttagc attcctgtta
    12541 tacggtcatg ttctcttgct gtaagagtta catattttgc tttggttccg caaaatagtt
    12601 cttgcagttt ttcaacatct tttttcgtct tttccaaagg tgtaaagatg taataggcat
    12661 tttcaaatag tttagcatct gctgcctgta tacctgattt atgagaacca gccattggat
    12721 gtccaccgac aaaccgaaga tttttctgct cagcggatgt cacgacttgc tgcttcgtac
    12781 tgctgacatc cgtaatgatg acgtgatcag acaagttgat tctttcaagg acatcaagat
    12841 aagctaaggt tgtacggacg ggaacggcta ataagataac gtctgcttgt tctgcacctg
    12901 tttcaaaatc acttgcaacg taatcaacga tctgctgttc tactgcttgc tcacgggttt
    12961 ttgactgact atcccaacca actactatca gttctggatt ggtctgacga atacaaagtg
    13021 ccatagaagc accaatcagt ccaagcccca cgataaaaac atgttgtgcc atcttatttg
    13081 cccccttttt agtaatgttt caagtagtca cggtactcct tcactgcagc tttcaactca
    13141 acaagcgaat cacttgggaa ttttgacaat agctctgtcg ctaccacagt ggctacaaca
    13201 ttttccgcaa cgacgctagc tgcaggaact gctgtactat ccgaacgttc tacacttgct
    13261 ttgtaaggtt cttttgtatc gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg
    13321 ggtttcatga ccccgcgaat gacaagtggt tcgccattgg tcattccgcc ttcgaagccg
    13381 cctagattat tggtacgtcg gaaatagcct ctttcttctt cccataggat ctcatccatc
    13441 acttctgagc ctggtaatga accagaagtg aatccgaccc caaattctac gcctttaaag
    13501 gcattgatac taaccacagc ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg
    13561 tagcttccta atccagctgg aacgcctcca acgagcactt ctacaattcc tccgatcgta
    13621 tctccatttt tctttgtctg atcgatcaaa tcttttattc tttgctcaac tgctggatca
    13681 acaactcgta cttctgacgc ttcagaaatt tctttgattt cttgaatcga gatatttcca
    13741 ggtatttctg cttggattcc accaagacaa atcacgtgtc ctgctacgat gatgtcaagt
    13801 tcttccagaa ttttcttggc aattgcgccg attgctacac gcatggtcgt ctctcttgca
    13861 gaggaccgtt caagcacatt ccgaagatct cgatgatggt atttcatacc gcctaccaaa
    13921 tccgcgtgtc ctggacgcgg acgtgcaact cgccgcagtg attggtcttt ttcaggcact
    13981 tctgctacag acatgacagt tgtccaattt ttccaatctt tattttctac cactaaagtg
    14041 acaggtgaac caattgtttt gccgtgacgg atgccagacg tgatccgaac acgatctttt
    14101 tcgatcttca tccttcctcc tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg
    14161 atttcttctt ctgacagagg taatcctgct ggtagtcctt caataatggc tgttaattct
    14221 ggtccatggg attctcccgc agtcatataa cgcatcttac agtcctccta agtaatcttt
    14281 gaatgtctct gtgggaacag gaacgatttt tgcttgccca atcttttcta gcaagatgat
    14341 attcaattgg cttcctcttg ctttcttatc atgggttatc gcgtggaata attcatctgg
    14401 atgacttaga tcgtacgtaa tcggcagatg gaatttttga agtatccttt ctaaagcagc
    14461 tgtcgatcca gttggagaca agccgatttt ttctgcatga gaagtgatgg ctaccattcc
    14521 aatagctact ccttctccat gactgactac cccatatcct gctgtatttt caattgcatg
    14581 gccaattgta tgaccaaaat ttaaaataag tcgatttcct tggtcaaaga catcttcttc
    14641 cactactttt cgtttgattt ccaacgtagc agtaatgatt ggaacagcat gttccagaag
    14701 atctttttcg tctttcagct cgttcagctg tttccaaagc gttgcatctg ctatagctgc
    14761 tgatttgatg atttcagcga ttccttctcg taaacgacgt accggcaaag tttttagaac
    14821 ctcaggatca atcaatacac cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt
    14881 gtttgtattg actgctgtct tcccgccaat actagaatct acttgtgcaa gtagcgtagt
    14941 cggaatttga acgaaatgga tcccacgcat gaacgtcgag gccacgaatg ctgctagatc
    15001 acccaccatt cctccaccaa gtgcaagtat cccatcgctg cgggtaaaat cattatctgc
    15061 taaaaaatcg tataactcag ctgcttgttc taaagattta ctggtctcac ccgccggtac
    15121 gattgaaagg actggttcaa aattagcttg tctcaattgg aacatgattt tttctgcata
    15181 tagtggagca acgtttgtat cagtgatcac agctactttt tgaggtctcc aaatcgtttg
    15241 aagccatgat ccagcctgtt ctagtgcgcc tttttcaata tgaatatcgt atttttggtt
    15301 atcaacatgt acagtaagca ttcgtttcac ttctttcttt taaaaagctg gaattaatcg
    15361 agtgtctgaa tcttttccat tgcttctttc atgatcgcaa cttctttcat catgcgtgag
    15421 taagtctttt cattcaacga ttggggaccg tctgaccaag catttgcagg atcaggatgg
    15481 atctctacga tcatgccatc tgctccgctt gccacacccg cacgagccat tggtggtaca
    15541 agttcccaaa ttcctgtacc atgacttgga tcaacaatca cagggaaatg agttaatttt
    15601 ttcatcaatg ggacagcact taaatcaaac gtattacgtg tcgctgtttc gtaagtccga
    15661 atcccacgtt cgataaaaat gacaggagat ttgtcttcga ctgcaatgta ttctgcagca
    15721 ttaagccatt cattgatcgt accggaaatt ccacgtttca atccaacagg tttacctgtt
    15781 ttaccgacag cagatagcag tttaaaattt tgcatattac gtgcgccaat ctgtaggata
    15841 tcgctgtact cagccaccat atcgatatgc ccttcatcca tcacttcagt aatcactttc
    15901 attccaaatt catcagccgc ttgacggatg tatttcaatc cttcttcttc taaaccttgg
    15961 aaagcatatg gagaagtacg tggtttgaag gcaccacctc gcagaatttt tgcgcccccg
    16021 gctttggcca ttcgcgcaca ttctctgatt tggtctaatc cttcgataga acaaggacca
    16081 gccatcgtca cgaaactgcc gtcaccaatt tttactccgt ctacatccac gactgtattg
    16141 ccaggatgga attctcgaga agtcaatttg tatgtgttta aaatacgcac agcattttct
    16201 actccttcat agcgtgtaaa tgctccttct tgtaaacttc gcgtatcacc ttttagtcca
    16261 atcactactc gttctgttcc ttgatcgatt gagactttga atccttcttt ttcgatatga
    16321 tcgacgactt tttttatttg ttcttccgtt gcttcttttt tcataattac tatcattgtt
    16381 tttcccgctt tcttttgttt acttatctat cttggctgat tttctctact ttttcaaggt
    16441 ggttttacat taattcctac taaatgaccg tgtcctgttg ttctcatttt cgctccttct
    16501 ttcctactta tgacccagat aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta
    16561 cagtgaacat cttcaacgag ccttaaaaag cgctgcaaaa atataaaata gccactatag
    16621 atgaatttct ctacgtggct gtaagtcgtg tttaaaaatc tttagccatc atagatacaa
    16681 acctggtttg tttgtaccca cgaagtcatg aatacaaacc tatctaaaat agctaaagta
    16741 atacgtattc agttttgtaa gttttctaga tttcataaaa aatcgctcca aactgatgta
    16801 agaattgtac ctagtataga cataaaaaaa tcgactgtca atcattttta tgaaactttt
    16861 tttaattttc tgatagttaa ctttgttttt cagaaaaata acaattggtt ttattgaaga
    16921 aaatagtcat gaacaaaagt atctgagaac ttttttactc tcttgttcat gacatataga
    16981 ataaagcagc tttgtcttgt aaactccgtt tttcaaataa taatctaaag attgctactg
    17041 aataataaag ccgatttcat ggtcctcttc gctgctgatt cggtaaggtg actcaacatc
    17101 cgctccccag ctattgatac ccccgactcc tcttactttt gcaaaaatcg taagaaccgt
    17161 tcgtctttct ggcggtagtt cttctatatg tgttgcctgt tccagttctt ctgctttgaa
    17221 tggtaaagcg gaaaaagcaa atggcgtatc ctgctgttca aatcgcaacg aaaaatcttc
    17281 ttgatcttta tccgtattat ccaacgtttg atttcttgtt agttctaccc atttcgtttc
    17341 catatgcatg ccgcattctt ggggaacaag gtatggggaa acggggagtc ctgccacttt
    17401 ataaatacct tcgacaccac cagccatccg atccggatat gtttcccctg ataggccttc
    17461 atactcaaaa aattttgctt tcgttggcat aatgaacctt aaacccaatg cgggtaattc
    17521 cggtaatcct ttttgtccat aataatgcat attgacaagc attttaccct ctccagcgat
    17581 ttggtaggaa attgtcactt gggtttttgg tactgtgttg atttccagtg taaaaatgat
    17641 ttcaaccgtt tcagcgtatt cattacttga atattgatta tttactggag caatcggcaa
    17701 atcgatcttt ttattgtcca ccagtacttc tatttctttg acttttggat acaatccggc
    17761 tcctaaccac ataccggatt taaatccaaa tccgctacct ctgtcattat cggtcgttgc
    17821 tcgccaaaaa actggaagtg tttcccggta tagccattct tttccgtttt tattgagcga
    17881 ttccagtccg cctcttgtat aagaaaaaat ataatgcgta ttccccgatt tgacacctaa
    17941 tgtacagtcg ccgaagacga ttgcaacttt ttccgtatcc atatgacttt tatttactgt
    18001 atttttcata ataatatctt acctcctgca tggcgggttt ttcttgtcca tctgcaaaaa
    18061 cgatcccatt ccctgagaat tcatagtcac ttggtttatc gtcaaagtca ccgccatagc
    18121 gcatcacttc tctgcctgtg attggatcag tcacttggat cgcctgatct ttaaaatccc
    18181 atatatagcc tccttggtac atttcataac gatcaagcaa cgtcatgtaa gaatccattc
    18241 cacctaacga attccccatg tcatgcatat attcacataa gatgaacggt ttttccgctt
    18301 tattttcaag gtagtcaatg atctcttctg gtggcgcata catgcagctt tttacatctg
    18361 aaatcgaatc ttcgaatgct ggatttggaa aattcccttc ataatggaca agtcgatcgg
    18421 ggtctacttt tttgaagtaa tcgttcattt gtcgaatgtt ttcttccgca taagattcat
    18481 ttccaagaga ccaaaaaaga atacttggat ggttttttaa tgtttcgaaa ttcgtttttg
    18541 ctcgatcaag aacagccgcc tgccactgag gtactgcacc tggtacgttg taagaaggct
    18601 caaatgcgcc cattttttgc catgagccat gggattcgag gttcgtttcc gccatcacat
    18661 ggattcctgc ttggtcgcat aaataatacc aagtgatctg gttggggtaa tgagatgtcc
    18721 gtacgccgtt gatctttgca ttttttatga tagccaaatc tttttccata tcttctatag
    18781 tgatggcgcg tccttttttc ggatgccact catgtcgatt taccccgttg ataatcatcc
    18841 gttttccatt gaagtagatg attttatctt tgatttcgat tttacgaaaa ccgaaggaat
    18901 agtcaatata ctccgttact cttttttctt cgtctatcgt ctccaattca agcttatata
    18961 aattgggctg aaaatgcccc cacagtgcca cattttcaaa tccatcgatg cgaatcgtca
    19021 tttgctcttt taacggatat tgctgttgat agatttcctt cccatcagga tctagtatcg
    19081 ctacattcag ctgactgttt ttggctactt gctcaaaaga caaatctact tcaagtttgc
    19141 cattttgaca tttgtcatct aagcgaggtt tgatccaaag atcttctaaa tggttttgtc
    19201 ctcgagcaac taagaagaca tctctgaaga tcccaaaaaa tcggaaaaag tcctgatctt
    19261 ccaagaatgc agctgtactt cttttgtaaa cctccacagc tagaatattt tcttcttcca
    19321 aataaggtgt taaatcaaat tcagacggag tgaacgaatc ttcggcatac ccgataaaat
    19381 gaccattcaa ccaaataaac attgcttgtt ctacaccagc aaaatgaatc gagatttgtt
    19441 ttccctttag accttcttct aatcgaaaag tcgtcaaata cgaaccaacc ggattgtctt
    19501 tagcagctga aaagatccct tccgcctctt tctcagggct tgtcgtgaaa gggggacggc
    19561 gaaatacatg tccctcccac ggatacatcg tattgatgta atggatcttg tcataatttg
    19621 ctaattcaat atgactagga acagtgattt catcaaaaga acaggcatca aaatcttttt
    19681 tataaaatgt ttttggtcgt accgaaggat ttggcgaata acaaaaacgc catgatccat
    19741 ttaaagactg tttaagggag gatgtggttt ctacttcttc ccttgttgca taacaaacat
    19801 gatcgctatg tgcatttgat tggttcactc taaaaacttg tggatcatct aaccatttta
    19861 tatttgcttt catacttttt tccttctctc tcataacttt ttgaaatgaa gcagttctgt
    19921 ttggaaatca ccttgaaaat cggggaagta aagtccaact gtcatcaatt cggcccccga
    19981 aaatatccgc ttcgaggtct gttcctgata aagtgcctca tcatctaacc ccatcagata
    20041 tacttcatgg aacgctggtt gagctgaagc tagtatcctc cctaaaaaca caatagcttc
    20101 atcttgttta ggagacacaa acatccatgc ggcaatattt tcttcaaaag gactcgccaa
    20161 acgataataa tggccatact ggatcaccgg acgaatcgtt tgataggata taatttggtc
    20221 ttttacttgc ttttcttctt caggagacag ctttgtcaaa tccagttcat aaccaaaaac
    20281 agcgctcata gctgtttctc ctcttgtttt caacgaagta gatctgtcgg tctgatgatt
    20341 tggtacagct gatacatgag ctgtgatgga cgaaatcgga taagcaaggc ttgttccata
    20401 ttggattttc atacgctcca ctgcatccgt gttatcactt gtccatgact gcggcatata
    20461 ataaataaac ccggcatcaa accgtccacc tccaccagaa cagccttccc ataagatttc
    20521 cggataatca gttgtcaaat gttcaagtag ttcataaagc ccgagcatat aacgatgcgc
    20581 tacttctcct tgtctctgtg ggtcaagggt aatcgaatag acatctgaaa gcgaacggtt
    20641 catatcccat ttgatgtagt caagaggaat cgtatctaaa atctttctca tttgtttctc
    20701 aatcgtctgc cgaacatcta accttgtaaa atccagtaca tgttgtgatc tggaagcaga
    20761 aggcattcta cctggctcct gcataagaaa atcaggatgt gttcgataca gttcagaatc
    20821 aaccgagatc atttcaggtt caaaccataa accaaatttc aatcctttgc tatgagcata
    20881 atctgctatt tcacgtaatc catttgtcaa cttgccttca tactcgaacc agtctcctaa
    20941 tgaagaattg tccgcatctc tttttccaaa ccagccatca tctaatacga acatttcgat
    21001 gcctagctcc tttgcttcat caatgatcgc cttgattttt tcactattga agtcaaagta
    21061 agtggcttcc cagttattga ccagaatcgg ccgttcagca aattgatgac gacctcttgc
    21121 gattctttca cgtagcaact catggtggat ctgactcatt ttgttcaatc cctgacttga
    21181 gtaagaaagg atcatttcag gtgtttgaaa agactggcct gcattcaatt cccacgaaaa
    21241 acgatggctg ttgatcccac cgacgactct caattgattg atctgatctt tttcaagttc
    21301 gaaagaatgg ttgccggaat aaacaaattg aagtccgatc gcttctccag tattttctgt
    21361 cgtatgatga tgaactaaag caataaagtt attcatatgg tgactgctcg ttcctcgacg
    21421 gctttcaaat acttttgttc cagataaaac agattgccgt gaaatctgtc tttcattgac
    21481 atgtgctcct ggcaaagcaa tgacttcatc aaatcgccta gtatgggcaa agtctagctg
    21541 gaaactagcc gctttttcta gtttgattgt ttctccggtt tcatttctta tctttacaga
    21601 tcgtgtgatc actgctctat cagtaaatat ggtatagaac aaatcgaaat aaatcgctgt
    21661 ttctctatcc ttaagtgaaa tcaccaacgt ttgtgcctct gacttgtcca tcacataagt
    21721 ttgaggcaag ccttcgatct ctggcttccc atctgtaata taatggctgt catatctcag
    21781 atccagtaag tctgatccat tcttgcgctg aatgatcgat gcgggcgttc gaaagtccat
    21841 gctgccatga gaagagtatt cttgcggtag tgtatctttc gacagagacc ggtcaggatt
    21901 taagggaaca tttcctgaaa atccacgatc tcttcgggga taatttttat tgtctttgta
    21961 ctgcttgact ggtttgccaa aataaatatg cgccaataca ttgctttctt ctatctttat
    22021 cagataagaa agaaatgtat ttcgtaaatg gaagactcgg ctcttttcat caaataagat
    22081 gaaattttta tctgtttttc tatgcgtaat atccatcatt tcactcccaa caaaacggaa
    22141 accgtttact tttttttata attcctgtat actgtttact aaacaatttt gtcaataaaa
    22201 attttttatt tttttgagga gtgtgtaaaa atggttggaa ttagagatgt cgcacgaaag
    22261 gcaaatgtct ctcctgctac agtttcccgt gtgttgaata acgatcagac gatccatgtg
    22321 gctcagacta ctcggcaacg gatagtagag gcagctgctg cgttggatta tgacatcagt
    22381 actcgtaaat atatcaaaaa gagaatgcct tcgattggtg tgatctctac gatcggcaaa
    22441 gcgagcgaag aacaggatat ctattacaaa catttgcgag tagggatgga agaagaagct
    22501 cgacgtctgc atttagggat gaatcgaatc tataatctat ctgataatcc taaacaatgg
    22561 gaagatctag atcagttagg tgcggtgatc gttgttggga ctgttacgaa agaatcgatt
    22621 accgccttgt taaaacaaaa catgcaaata gtcgtcgtgg ataaccctga catccagcaa
    22681 gaggtcgata tggtgtatgc agatcttgaa cggatgacta aaaccgtact ggaattgttc
    22741 ttagaaaatg gtcataaaag aatagcctat atcgggggat ataaagtcga tatggatgaa
    22801 ttcggtggga agcagatttc tataaatgaa aagaggatgc gtgcctatca gcattttatg
    22861 atcgatcatc acttagaaaa atatatgtct tatcatctag gtggctggac agaaaaacaa
    22921 ggtgaggtac ttgcgactaa tctattagaa aatgaatccc ctcttcctac tgccattctt
    22981 gtcgggagcg atccaatggc aattggtgtc tatcgggcat tgaaacaaaa aaatatccaa
    23041 attggagaag agattgttgt agcaagcttt gataatatcg agacaatcgc aaacctctcc
    23101 cctggcttga ccagtgttca aatcaatgcg aaatcaattg ggcaagcagc tgttcgaatg
    23161 gcattggagc gaattgacgg tatacgggac gaaccaatca ttttgactta tcctgcacga
    23221 ttgattgtta gggaaagttt tatcccaaga acaaaaaggt attgaaagag aggttgtgaa
    23281 aagccgcttc gtttcttcat actcaaaacg aaacggcttt ttcacaacct cttacttata
    23341 cttattcaca aaatccaatg tctgctggta acaattttgc gattctttta gcgtgtaatc
    23401 aaattggtat tcatgggtaa tctctttttt tgtatctgta taaaacaagg attgcacggg
    23461 tacctttaat tctgtcaacc gattttctaa tgcttgacct tgctcctgga atgagtatgc
    23521 atttccgtca gttatatacg ttggcggaaa ttccttagaa acatggtcag ccacactagc
    23581 ttctttcaat tgctcactcg ttttccaatc ttttgttcca atcaacgacc acgcaacggt
    23641 tttgacgaaa aacttcataa atcgatcatc cgaatgctgc tgtgctgtta gcttcaaatc
    23701 aacaggtccg caataagaaa tggttccttt cagactatct tttggaagta gctgtttgat
    23761 tttcatttgt ttagcatacg catcattcgt ttgtatagct gcatattgca atgcgatttg
    23821 tgaacctgca ctatctccac caaaaaataa ttgttccatg tctagcaatt tgtcgtcttt
    23881 ttcttcagct aataaatctt tgatcagttc attcacttga agaacttgat tagggtattc
    23941 tgaatcaggc gctaattcat aattcatcgc aaccactgcg atgttggcgt ctgaaactaa
    24001 ttttgtcgca aattctttga cacccgattt atctccgccg acgaatcctc caccatgtac
    24061 ccaaaataga acaggaacag gccccttgct gttttttgga tagtagatgt cataagtatt
    24121 ctctttatac ttggactcat acgtcaaatc tgtttttttc gccacgtttt tttctgcttg
    24181 atcgaacgtt tttttatcag tgatttgaac agtatttgaa aacatatggt tgatcaagaa
    24241 tgctccaggt ctgggggaaa gctggaaggc aagaaagatt gcaaacgcga tcccaagaat
    24301 gattccgaag aaccatccga taaatttaag aattttcttt ttcataaggc tccctcagta
    24361 gttagtatta agcattctga ttataccata gtgttatttc ctatcaagct ctcctagatg
    24421 atttaatgat tttccccaaa cttcttctgc aatgcttttg acaaaagaaa ttttttccat
    24481 tgttcttcaa acaaaacatt ttcttcttct gttgaagcaa atccacattg aggacttaag
    24541 cacaaacgat ccaaaggcag atatcgtgtc gcttcgtaga tccggtcaat cacttgctgg
    24601 tgctcttcca tttcgccatt tttcgttaca gtcaaaatct aagacaacta atttatcttc
    24661 agagactttc tctaaaagaa caaaactccc agcacgttca ctatcgtatt ctaaataata
    24721 ggcacggaca ttttcttgag taaaataatg ggtctgctac gtcgtcatcc cccctgatgc
    24781 cgccaaaatc gaataataat ttccgcgtca aacatgtata tttagtgttg aatcatttga
    24841 gaatatgaaa ataaaaatct ttttgatcaa tcagttaatc aaattgaaat aacgaaaaat
    24901 ctgagacaga atgacgttca ttctgaccca gatttgattt tgcacaactc aacagtcctt
    24961 attggcaaat cgccaaaata atgtagacta actaaaaaga ctaagaaaca ataacaaaag
    25021 ttaacacttc ttagtctttc tttagcacgt ctatttatta aagattttag ggccttttct
    25081 catgttttta cgatcattct taggtgtttc catttgtttg gatcggcctc caccttgttt
    25141 ctttttgatc aattctttca ttttttcttt ttggtccatt tttattctcc tttaacgaat
    25201 gaagcgacta attcgatatg atgggtttgc gggaaaagat ctacaggctg aactttcttc
    25261 acggcataac ctgcttctgc aaataactta gcatctcttg caaaagttgc cggattacat
    25321 gagatataga cgattcgttc tggtgacatt tcgattgctg attcaataaa atgatgatct
    25381 aatccttttc gtggagggtc aactacgatc acagatggtt tgatcccttt ttcttgccat
    25441 tttttcataa ctttttctgc ttttccgact tcataatgaa cattttcgat tttattattt
    25501 aaagcattga actgtgcatc ttcaattgct tctggaataa cttccatccc atacacctta
    25561 tccactctgt ctgctaaaga caagccaatc gtacctattc ctgaataagc gtcgatcaca
    25621 atgtcttttt tatctaactc ggcaaactcg atggctgttt ggtacagata ctctgcttgt
    25681 tgagtattta cttgataaaa tgactttgct gagattcgat aggtttttcc taatagctga
    25741 tcttcgatat agtctctgcc atataaaact ttttctttgt ctcctaaaat cacatttgtt
    25801 ttttcttcat tgatattttg gatgaccgaa accacttctg gcaattcttc atgaatagct
    25861 tcagcaattt ccttggattt aaagaatttt tctttacgag taactaaaac gaccatcatt
    25921 tcatgggaat aatgaccacg acggataatg atgtgacgaa gctgtccttc atttttttct
    25981 tcattatatc ctcgtacttg gaaccgttgc aagatatctc tgactacttt gattgcttga
    26041 tcgatgacag gatcttggat ataaaaatct tcgataggga ctagatcatg gctattcttt
    26101 ttgtaaaaac cggtggctaa ctgtccatcg atacggcgaa cagggatctg tgctttatta
    26161 cggtagccga atggttctgc cattccgacc gttggaagta caggaacttc tggcatctta
    26221 gcaatcttcg ccattacatg ctctacttgt ttttgtttga agacaagctg ttgttcatac
    26281 ttcaaatggg ctaaaggtgc gatacctatg cgaagtaatg cattgttttc tacaggctga
    26341 cgatcaggtg attgtgtaat aaatgtttcg acttttccat atccaaactt attgcctgct
    26401 tttactacta gtatttctac ttcttctcca gggagtgcat tttcgataaa taatggataa
    26461 ccatcgattt ttgctacccc cattccttca taactcaagt cgacgatttc gactgtatat
    26521 cgttcgtttt tctttactat ttgctgttct gtcatatttt gctcctttaa gcctattttt
    26581 caatcctact gaactctact ataaatcagc taattacaca actaaaaccg gcactgacct
    26641 tactacgttc agcaccggct tttgattaat tcttttgatt aggtgtttcg tctgctattt
    26701 ttccgtcacc gtcgatatct tcatccgtta aacgttcgac ttcttttacg aattctttgc
    26761 tgacaacctc taattcttct tcatctgtac ctgtgattgc ttcatcagga atctgatcgg
    26821 tattggcaaa gaactcaatg tgttgatgaa gattttggaa atgcatcggt gcttccccgc
    26881 cgtattcacc atctaagttg atcattactg gtcgattgat atcatcttct gtttcaacga
    26941 acaaactgct tgttttggta tagattattc ttggatcatc gacatgtcga ccgccattca
    27001 gcatgagtgc tgcaatatgc aagatctcaa agatatttgc tgtcttcaca atgattaaag
    27061 aaaacttccc atcatccaat ttggcatctg gcgcgatctg ttcgaatcct ccaacagagt
    27121 tcgtcagccc taggaaaaac atcgaagcat ttccgatata ttccccttca tcatacttca
    27181 agcgcatttt gattggcttt actcgaggga gcatctctgc tcctttcgca agataagcaa
    27241 gataaccaaa aatactcttt agttctgatg gaacttcata agtcaattct gtcaaatagc
    27301 cacctgcagc gatattgatg aagtaactgt tatcagaaag gccgatgtcc atttttacgg
    27361 tttggtgttt ttttatcact tctgctgctg ctttaatatt atctctagga atttttaatg
    27421 ctcgagcata gtcgttcgtt gtaccagctg ggatgatggc cattttagga cggtgttcaa
    27481 gcggagcgat tccattaacc acttcgttga ttgtgccatc gccaccagct gccactacta
    27541 gatcaaaacc tagttctgcg actctacatg cttcattctt ggctgaatct ggttctggtg
    27601 tagttgcata ggcgctggct tcaaatcctg cttcttctaa tgcctgtagg atatccgcca
    27661 aatttttttt caataattct tttcccgagg tggggttata aattacgcgt gcttttttca
    27721 ttttcagcct ctatcgtttc gctaattcat ctttaagtag cttattcacg acaccaggat
    27781 ttgcttgtcc tttagttgct ttcatgattt gaccgactag gaagccaacc gcacgatctt
    27841 ttccattttt gaaatcatcg acagattgtt ggttgttatc caaaatttca ttaatgattg
    27901 gcagtagttt gttcggatca gataattgca cccaaccatt tttttcaact acttcttggg
    27961 cttctccgcc atttgtgatc aattcacgga agactttttt ggcgattttt gagctgatcg
    28021 ttccatcggc gatcaaacga atcattcctg ctaagttctc aggtgtcaat tttgtctcag
    28081 ctaattccag tttctcactg ttcaagtaag cagaaacttc acccatcaac cagttagatg
    28141 cttgcttggc atctgcacca ttttccaaaa cagcttcaaa aaaatcagac atttcttttg
    28201 taagtgtcaa taccattgcg tcatactctg gaagtccaaa ctcacggata tagcgttcgc
    28261 gacgagaagc tggcatctca ggcaaactag cttttacttt ttgaatccat tcgtcatcga
    28321 tcacgaattt cgggatatca ggctctggga agtaacgata atcactagac ccttctttga
    28381 cacgcatcaa aactgtttta ttcgttgttt cgtcaaaacg gcgtgtttct tgttggattt
    28441 ctcctcctga aagaagaact tttgcttgac gtttttcttc aaaagctaaa ccttttttca
    28501 cgaaagataa agagttcaag tttttcaatt ctgctttcgt accgaactct tcttgtccat
    28561 aagggcgaag agaaatattg gcatcacagc gcatagagcc ttcttccatt ttcacatcgc
    28621 tgacttctgt aaattggata attgatcgca atgcttctag ataagcgtat gcttcttctg
    28681 gtgaacgcat atcagcttca gaaacgattt cgatcaaagg cgtgccttgt cggttcaaat
    28741 caacgtaaga ataaccgcca ataccatgca tgttttttcc agcatcttct tctaaatgga
    28801 cacgttcgat ccggattttt ttctttttac cttctacttc gatttcgatc catccgtcat
    28861 gaccgattgg ttgatcaaat tgggaaattt gatatgcttt tggattatct gggtaaaagt
    28921 aatttttccg gtcaaaatga gtatctttag agatttcaca gtttaatgcc aaagctgctt
    28981 tcatcccgaa ttccagcgca gctttattca ttactggcag cacgcctgga taaccccagt
    29041 cgatcacgtt tgtattgctg tttggttctg caccaaaatg agctggcgct ggagagaaaa
    29101 tttttgagtt ggtttttaac tcgacgtgga cttctagtcc aatgactgtt tcaaaattca
    29161 ttcgtctttc cctcctaaaa tagctggttg ttgtttatgg aattctgttg cttgttcaaa
    29221 cgcatatgcc gcttgataca tcgttttttc atcaaaataa ttaccgatga tctgcaatcc
    29281 aactggtaat ccttctgata aacctgccgg tacagacata cctggaagtc cagccaagtt
    29341 gacagggatc gttaagatgt cactcatgta cattgtgatt ggatcgttaa tgttctcacc
    29401 gattccgaat gcaactgttg gtgaagatgg cccgatgatc aagtcataat ctgcaaagac
    29461 tttttcaaaa tctcgtttga tcaatgtacg gacttgtcct gcttttttga agtaagcgtc
    29521 ataataaccc gcgcttaagg agaatgtacc taacatgata cggcgtttta cttcttcacc
    29581 gaatccttct gaacgtgagt tgacatatac atcatctaat gatttcacat tttcagagcg
    29641 ataaccgtag cggataccat cgaatcgttg caaattggaa cttgcttctg atgaggcaat
    29701 gatataataa acagccacac cgtattttga atgaggcaag ctgacttctt ccacaacagc
    29761 acctaatgaa cggaatgttt ctgctgcttt caatacagct tcttttacgc caggggccac
    29821 accttcgcct aagtattctt taggtaaagc aatcttcatt cctttgatgt cgcctgtcag
    29881 tccctctgca aaatcaggaa ctgaaacgcc tgaagaagta ccgtcttttt cgtcataacc
    29941 gctgatagct gtcaatgcta atgcattatc tttcaccgta cgggtcattg gtccgatttg
    30001 gtcaagagat gaagcaaagg caatcaaacc aaaacgtgag acacggccat aagtaggttt
    30061 catccctacg atgccgttaa aagctgctgg ttggcggata cttcccccag tatcactacc
    30121 taaagaaact ggaatttgtc cagctgcaac tgctgctgca gaaccaccag aagagccacc
    30181 aggtactttt gttgggtccc aagcattttt tgttttcttg aaataagaag tttctgtact
    30241 tccacccata gcaaattcat ccatgtttag cttaccaaca gggatcatat ctgcttgata
    30301 gattttatcc atgaccgttg catcataaat tggttcaaaa ttgtaaagga tcttagatgc
    30361 agcggttgtc aagatatctt ttgttacgat attatccttg ataccaattg gaataccagc
    30421 aaggacgttg ctttcatcaa tccctttttc atctaaagct tttgcctgtt ccaatgcttt
    30481 ttcttcgttc aatgtaatga atgcctcgac ctcaggctct gtttctttga ttcgttcaaa
    30541 tgttgttttt gtcaaatctt gtacagtgat ttctttcgat acaagcatat gatgcagttc
    30601 ttctagagaa cgattgtgta attctgtcat tatgcgccag cctccccatt atcgatgatt
    30661 gcgggtactt tgataaagcc gtcttctgtt tccggcacat ttttcaataa ttcatcgcgg
    30721 ttccatccag cttgcgcccg atcttgtcgc atcacgttta ctgtttcaac cacattcgac
    30781 gtaaatggaa caccttccgt atctacttct tccagcattt caaccatatc gatgatttta
    30841 cctaattggt cggtaaaact tgcaagttct tcatctgcga acgcaagttt tgctaatttg
    30901 gcaacgtgtt tcacttgttc ttcactaatc gccatactac cccttctttc taaacgagtt
    30961 aaaaactttg ctctctattt taacacaaga gtgccgtgtc tgaaattgtt tattgaaatt
    31021 tgtctgaaaa aactcatttt tcattcttct actgagcgac ttttagccaa tgcatctgcc
    31081 atttgatctt ctgtccaaac ttcgattcct aattcttgtg ctttggtcag cttgctgcca
    31141 gcatcttctc ctgctaccac gatatccgtt tttttcgata cgctaccagt cactttcccg
    31201 ccaagatttt cgatcgtctc ttttgcttct tcacgagtaa atcgtgttag tttaccagta
    31261 aggactactg ttttttcttt aaaaggagac tcgacttctt gcaattgggc atttcgaagc
    31321 cctttgtatt cgaaattgac gcctgcttgt tttaattcat tcatcaattc atggacttct
    31381 tcgttactga aataagtgac tacactgtcg gcaatgatat caccgatcgt atcaagcgca
    31441 ataatcgact catagtcact tttactcaat gtttctagat caccgaaatg ttctgctaaa
    31501 atttttgctg ctttcgctcc gacatgtcgg attcctaacc cgaaaatcaa ccgctcgacc
    31561 gaattatctt tgctgttgtc gattgctgtc aaaatattat ttgctgattt ttcttttatt
    31621 ttgtccaacg tcaataactc ttcttctgtc aatttgtaaa gatcagcaac atctgctact
    31681 agttttttat catacatctg ttctagtacg cgcggcccca acccatcgat attcatcgca
    31741 tttcttgaaa caaagtggtt caaaccttct ttcatttgag cagggcattt aggattgatg
    31801 caccgtaagg ctacttcttc atccaaatgg acaagttcac ttccacaaac tggacaatgt
    31861 gtaggcaatt ggtattcttc gctgtcttta ggtcgtttat ctaacacgac ttgcgaaact
    31921 tctgggatga tgtctcccgc tttataaatc agaactgtat ctttcaagcg gatatccttc
    31981 aacttaatat aatcaccgtt atgcaggctg gcacggctta ctgtcgttcc tgctactcgg
    32041 actggtgtca tgatagctgt tggcgttact acccctgttc gaccgactgt ccattcgatg
    32101 ttttcgatta gcgtttcaac ttcttctggc ggaaacttgt aagctgctgc ccatctcggt
    32161 gctttgatag taaaaccaag ttgatcttgt agcgagaatt cattcacttt gatgacgatt
    32221 ccatcgattt catagggcaa atccacacgt ttgtcatgat attcttcaat gtatgaccac
    32281 acttcatcaa tcgaatgaca tagacgtttt tcccgattcg tgtggaatcc aattttttct
    32341 agctcttcta gcgcatcata ctgggttttt gcttgcatag gaccgaaatc agctactgtg
    32401 tataaaaaag tgtccagatt tcttttcgct gtgattttag aatctaactg acgcaaagaa
    32461 ccagctgcgg cgttccgagg attcgcaaag atatcttttc cctctgcttc tctgtcttgg
    32521 ttcaattgga caaatgaccg ttttggcatg aaacattcgc ctcttacttc gatgttcatt
    32581 ggttctttta atttgattgg gaccgaccgg acagttttta ggttttcagt gatgttttct
    32641 cctacagttc catccccgcg agtcgcacct ctcacgaaat ttccatcttc ataacgcaaa
    32701 gaaacggaaa gtccatctat ttttagttca cagcaatagt caactacacg tcccactgct
    32761 ttttgtacac gttgatcaaa agctatcagc tcttctttgc tgaaaacgtc gttcaaactg
    32821 taaagcggaa tatcatgagt gactttttca aaaccttcta atacttttcc gcctactcgt
    32881 tgagtgggag aatcagacgt gatcaaatca ggatattgtt cctcgattgc tactaattct
    32941 gcgtattctt tatcataaac atagtcttcc actgttggtt tatcttctac gtagtattcg
    33001 cgagaccatt ggttcagtct tgtacgaagt tcttctgctc gtttagcagc ttcgtcaatc
    33061 gtcatttcat gttccatcat ttaccctcca ctactaagct ttttcaattg gcgcaaaggc
    33121 agccaaaaga cgttttactc cttgacttgg gaaagccaca tctagttcta gatccttcga
    33181 cgtcccgctt actcggacga ctgttcctag accccatttt ttgtgtttga ctttatctcc
    33241 aggtttccag ctgttatttt cccctccagt agcactttta gagggaacaa ccgctgtctc
    33301 tggctgttta tagctatatg ctgttttttt agcgaataca ggcgtttttt taggcgtagg
    33361 acgcatgcct tcgatttcca gtaactcctg atcgatttct tctacaaaac gagaaggtcg
    33421 gttatattgc gtccgtccat ataatgttct ggagaaagca tttgttaaat acaacgcttc
    33481 ttctgctcgc gtgatcccca catatgcgag acggcgttct tcctccagtt cgctttcttc
    33541 catcaatgct cgagataaag gaaaaacccc ctcttctaga ccaatcagga atacaactgg
    33601 aaattctaat cctttggctg catgcaaagt catcaatgtc acttgggaag catcttcttc
    33661 taaattgtca atgtcggaaa caagtgccag atcatttaag aagaccgtta gtttttcttc
    33721 tggtgcatca gcgtcttctt cattttgctg ttcgaattgt ttgtcgaatt cttgtgtgac
    33781 cgttaaaaat tcctccaagt tttcgatgcg tgcttgtgct tctaatgtat tttggatttt
    33841 taagtcttct agatatccgc tacgatccaa aacctctttc gtcaattctg tcactgtcaa
    33901 gtatgggatc atctgagtga cttcttggat catctctcca aatgcaccta attgctgtcc
    33961 tgcttttcca ccgatatttg ccagatccac attttgcgct gcttcaagca gtggccattc
    34021 atgaagagaa gcaaaactgc gtagtttttc gatagatcct ggtccgatgc ctcgtttggg
    34081 cgaattaacg atacgctcaa aactgattga atcttgagga tttaccagca cattcaaata
    34141 cgctaaaata tctttgattt ctttacgatc gtagaacttg tgccctccaa ccattttata
    34201 aggaatattt gctttcaaca gagtttcttc cattacacga gattgtgcgt tcgtccggta
    34261 caagatggca aagtcgccgt aattccgatg attgcttcga atctcttctt gcatccggtc
    34321 tacgataaaa cgggtttcgt ctcgttcatt atctgcacgg tagtaagtga tcttgtttcc
    34381 ttctttgttt tctgtccaca agttttttgg ttttcgattg ctattgtttt caatgacttg
    34441 gtttgctgcg ctaaggatgt ttttcgtgga gcgatagttt tgttctagca aaattactgc
    34501 agcatctgga taatcttttt caaaatccaa gatgttctgc atatctgcac cgcgccagcc
    34561 gtaaatactt tgatctgcat ctcccacgac gcacaggttc ctaaatctac ctgccaataa
    34621 attgactagc gtatactgtg cgtgatttgt atcttggtac tcatctacat gaatataatg
    34681 gaatttgttt tgataatacg tgagtgaatc aggatgttct tcaaataatc gaatcgtatt
    34741 catgatcagg tcgtcaaagt ccatacattg attatattga agctcttttt ggtaagcagc
    34801 ataacatttt gccgcaatct cttcgtaata actgccttgc atttttgcaa aatcctgagg
    34861 ggtctgcagg ctgttttttg cttgagagat tgttcctaat atacttcgag gatcatattt
    34921 tttcggatca atgttcagtt cttttagtat ccgtttcatc aaggtcagct gttcagaaga
    34981 atcgatgatt gtaaagttcc ggttataccc gataaaatca acatctcttc ttaaaatacg
    35041 cacacacatc gagtgaaaag tcgataccca gacatcttcg cctccagatg cgagaatcgc
    35101 attgacacgt tctttcattt cttttgcagc cttattggta aaagtaatcg ctaggatgtt
    35161 ccaaggattg acttcttttt cctcaatcaa ataggcaatg cggtgtgtca gcactctcgt
    35221 ctttccactt cctgcacctg ccattagcag taatggtccg tcagtgtgaa gaacagcttc
    35281 tttctgacgg gggttcatcc cgtttaataa ttcagctttg gatgacatta acaacatcct
    35341 ttcttttatc aatcttgtta attatagcat tttcttggct ttcattaaac tagctagttc
    35401 aaaatcattt gtttcttttt atggattttt ccttgtcaat tcgttgattt cgcgctaaat
    35461 taaaagaaag cgaggcgttt gaatggaaaa aaaaggaaaa atcaagaaat tagataagac
    35521 tcagatatat tttattctaa aaggaatatt tgttggactg ctgacaggaa tcgtcgttag
    35581 cctgtttcga ttatctatcg aaaagctaag cgatttagtt cgaagtatct atgaaatgag
    35641 tcgggaacag ccgatttact taattggcct agctatttgc tgcatccttg cagcattttt
    35701 cgtgggttat ctggtcaaaa atgaaccgga tatcaaggga agcgggattc cacaagtagt
    35761 gggacagctt cgcggggaat tatcaatgaa ctggttctct gttttatgga aaaagttcat
    35821 tggcggtgta ctttcagttg gtgcgggcct ttttctaggt agagaaggac cttctataca
    35881 gcttggcgca tccattggac aaggagctgg acaattgttt cggagtccat cttcagaaga
    35941 aaagatttta atttctagcg gtgcaagtag cggacttgca gcagcattca atgctcctat
    36001 cgcaggatta ctctttgttt tagaagaaat ccatcactcc ttttctccac ttgtttggct
    36061 gacatcattt gcttcagcaa tcactgctaa cttcgtctct ctttactttt tcggcttgag
    36121 acctgtttta tatctaggtg accttccttc tttgccgttg aaatattatg gttccttagt
    36181 gatcttagga gtacttctag gcatcttagg ttttatttat cagaatgtct tgctggcttt
    36241 gccaaaattt tacaaccgat tgcctttacc agcttatttt cacgggttcg ttcctttctt
    36301 gctgattttg ccaattggtt atttatggcc tagtactcta ggcggaggca atcaaatcgt
    36361 tttagctttt ggacatgctt ccttgccaat cattatgatt atcggtttgt ttgttcttcg
    36421 attcgttttt tccatgattt cttacggtgg gggtcttcca ggggggattt tccttccgat
    36481 attgacattg ggggctttaa tcggtagctt ttatggaaac attatggttg atttcgggat
    36541 ggatccaatc tacatcaagg attttgtcgt tattgccatg gctggttatt tcacagcgat
    36601 tggaaaagca ccgctgacag caattatttt agtgacagaa atggttggct ctcttaatca
    36661 tctgatgcct ttaggattgg ctgctcttgt tgcttatatc gtcaatgacc ttctcggcgg
    36721 taatccaatt tacgaatcat tattggaacg tctacttgga aatactcaga ccgcctcact
    36781 aaaaggtgca aggacaactt ttgattttcc agtaacggca gagagttctt tagacggatt
    36841 aatggtacga gacttcaatt ggcctaaaaa tatgctgcta atctctatac gcagaggaaa
    36901 tcaggaaatc ttgactcacg gtgatacgat catgaatgta ggagatattt tggttatctt
    36961 gactgatgaa gcaaacttgc caagaatcaa gcaggaaatc gaggctaaat cttctatcac
    37021 aaatttgaag caatcatttt aacgaacatg gaaaaactat tttacaac
//
LOCUS       DASNNU010000019        36377 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.019, whole genome shotgun sequence.
ACCESSION   DASNNU010000019 DASNNU010000000
VERSION     DASNNU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.019"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..496)
                     /locus_tag="U8116_000896"
     CDS             complement(<1..496)
                     /locus_tag="U8116_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN8433908.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYI"
     gene            671..2095
                     /locus_tag="U8116_000897"
     CDS             671..2095
                     /locus_tag="U8116_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN8433909.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2394..3101
                     /locus_tag="U8116_000898"
     CDS             2394..3101
                     /locus_tag="U8116_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN8433910.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3088..3411
                     /locus_tag="U8116_000899"
     CDS             3088..3411
                     /locus_tag="U8116_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN8433911.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3500..4537)
                     /locus_tag="U8116_000900"
     CDS             complement(3500..4537)
                     /locus_tag="U8116_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN8433912.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(4858..5841)
                     /locus_tag="U8116_000901"
     CDS             complement(4858..5841)
                     /locus_tag="U8116_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEN8433913.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6256..7026)
                     /locus_tag="U8116_000902"
     CDS             complement(6256..7026)
                     /locus_tag="U8116_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433914.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7019..7954)
                     /locus_tag="U8116_000903"
     CDS             complement(7019..7954)
                     /locus_tag="U8116_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8433915.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8270..9919)
                     /locus_tag="U8116_000904"
     CDS             complement(8270..9919)
                     /locus_tag="U8116_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN8433916.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10036..12639)
                     /locus_tag="U8116_000905"
     CDS             complement(10036..12639)
                     /locus_tag="U8116_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN8433917.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12651..13700)
                     /locus_tag="U8116_000906"
     CDS             complement(12651..13700)
                     /locus_tag="U8116_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEN8433918.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13726..14040)
                     /locus_tag="U8116_000907"
     CDS             complement(13726..14040)
                     /locus_tag="U8116_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEN8433919.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14056..14502)
                     /locus_tag="U8116_000908"
     CDS             complement(14056..14502)
                     /locus_tag="U8116_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN8433920.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGENIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14503..16425)
                     /locus_tag="U8116_000909"
     CDS             complement(14503..16425)
                     /locus_tag="U8116_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEN8433921.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(16637..17533)
                     /locus_tag="U8116_000910"
     CDS             complement(16637..17533)
                     /locus_tag="U8116_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN8433922.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(17526..18206)
                     /locus_tag="U8116_000911"
     CDS             complement(17526..18206)
                     /locus_tag="U8116_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN8433923.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(18207..18989)
                     /locus_tag="U8116_000912"
     CDS             complement(18207..18989)
                     /locus_tag="U8116_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433924.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(18982..19899)
                     /locus_tag="U8116_000913"
     CDS             complement(18982..19899)
                     /locus_tag="U8116_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN8433925.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(20063..20386)
                     /locus_tag="U8116_000914"
     CDS             complement(20063..20386)
                     /locus_tag="U8116_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433926.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            21174..24401
                     /gene="fss3"
                     /locus_tag="U8116_000915"
     CDS             21174..24401
                     /gene="fss3"
                     /locus_tag="U8116_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN8433927.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            24514..24828
                     /locus_tag="U8116_000916"
     CDS             24514..24828
                     /locus_tag="U8116_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN8433928.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            24841..25215
                     /locus_tag="U8116_000917"
     CDS             24841..25215
                     /locus_tag="U8116_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN8433929.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            25225..25560
                     /locus_tag="U8116_000918"
     CDS             25225..25560
                     /locus_tag="U8116_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEN8433930.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            25643..26983
                     /locus_tag="U8116_000919"
     CDS             25643..26983
                     /locus_tag="U8116_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN8433931.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            27061..27735
                     /locus_tag="U8116_000920"
     CDS             27061..27735
                     /locus_tag="U8116_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN8433932.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            27728..27892
                     /locus_tag="U8116_000921"
     CDS             27728..27892
                     /locus_tag="U8116_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433933.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            27968..29110
                     /gene="dcm"
                     /locus_tag="U8116_000922"
                     /pseudo
     CDS             27968..29110
                     /gene="dcm"
                     /locus_tag="U8116_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            29100..29390
                     /locus_tag="U8116_000923"
     CDS             29100..29390
                     /locus_tag="U8116_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433934.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            29649..30833
                     /locus_tag="U8116_000924"
     CDS             29649..30833
                     /locus_tag="U8116_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN8433935.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            30830..30967
                     /locus_tag="U8116_000925"
     CDS             30830..30967
                     /locus_tag="U8116_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN8433936.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            31713..33623
                     /gene="ltrA"
                     /locus_tag="U8116_000926"
     CDS             31713..33623
                     /gene="ltrA"
                     /locus_tag="U8116_000926"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN8433937.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            33727..33951
                     /locus_tag="U8116_000927"
     CDS             33727..33951
                     /locus_tag="U8116_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433938.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            33964..34467
                     /locus_tag="U8116_000928"
     CDS             33964..34467
                     /locus_tag="U8116_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN8433939.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            34527..34916
                     /locus_tag="U8116_000929"
     CDS             34527..34916
                     /locus_tag="U8116_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN8433940.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            34903..>36377
                     /locus_tag="U8116_000930"
     CDS             34903..>36377
                     /locus_tag="U8116_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN8433941.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT        11377 a         6870 c         6919 g        11211 t
ORIGIN      
        1 tgatataagc atatacagat ttctttaaat gttttggttc caagcctggt atgactgcta
       61 atggcatgaa tgtttgctca atcgagtatg gttgacaatc aataaaacgg cttcgtacaa
      121 tattatatac atagtctttt acactgatac ttaatttatc agctactttt ttcgatggaa
      181 gttcaattga gaaagtatgg atttctgaat ctacgtgttc ttcaccaacc acgctaaaca
      241 aaccttcgtt tggaccaaga actcgtgagt tatcaattgg tgttctgacg acttcagtcc
      301 catacccact tctacttcgc aaaactcctt cggaaacaag taagtctacg cctttcttta
      361 ctgttaactt gcttgtatga taaataactg ccaattcatc tcctgtagga agtttctcac
      421 caattttata ctctccattt acaatttttt tctttatatc taagtaaatt tccatatact
      481 tcgcaatttt tttcaatggt gaccctccaa tcaaaaacat atcttcatct atactttaac
      541 ataaataaac cgataaaatc cacatcaaaa ttatacacac aataaaaaag atatatatat
      601 ttaaacaaaa ctatttactt taagttgtaa gcggtgttat cattaagtgg aaacaggagg
      661 taaaaaaatt atgacaacta tgaaaaagct aacttttcca gatgattttt ggtggggttc
      721 cgcttggtca gctgagcaag cagaaggaaa aggagaaaca ggaaaagcag agactatctg
      781 ggatcgctgg tttaaagaac aaccttaccg gttttatcaa cgaatcgatt cctctattac
      841 aaccgatcac atccatcaat ataaagaaga tatagaacta atgaaaaaaa ctgggcataa
      901 ctcctttcgc gtttctattt cttgggcacg aatgtttcca aatggcggta ccggaaaagt
      961 aaatcccaaa gccatttctt tctatcgaaa attattttcc gaaatgaata ataacggaat
     1021 caaagtattt gccaacttat atcattttga catgcctgct actcttcaag ataaaggagg
     1081 atgggaatca cgtgaagtag ttgatgctta tgttcattac gctgaaactt gttttaaaga
     1141 atttggtgac ttagtttgtc attggtttac cttcaatgaa ccacttgggc ctattttagg
     1201 tacttatctt gaagattttc attatccaaa cctcattgac tttaaacgcg gagcgcaagc
     1261 agcattcttc actatacttg cacacgcaaa agcaatacaa gtatttaaag aactagctct
     1321 tcctagtaaa ataggcgtta ttctaaattt gagtcctact tttcctcgta gtcaaaatcc
     1381 tgctgatgtg aaagcagcag aaatcgctga tttattttat acgcggagct ttttagatcc
     1441 tatggttaaa gggaattttt ctaaaaagct tatcctgtta ttacaagaat ataatcagct
     1501 tcctgaagat tatacgaaag aagatcttgt atgtatctac gaaaataccg ctcaaattct
     1561 tggactaaac tattacgagc cacgtcgcgt caaatctcgt tcttctatgg tggatacatc
     1621 tagtccattt ttaccagagt ggtttttcga accacatatc atgcctggac gacgaatgaa
     1681 cgaataccgg ggatgggaaa tttacgaaaa aggaatttat gatttgtgca tggatatcaa
     1741 gaaccattat ggaaacatcg aagcatttat ctcggagaac ggaatgggcg ttgcggatga
     1801 agaacgtttc atggatgata cagggcaaat aattgatgat tatcggattg agtatatcaa
     1861 agatcatctt gcttacttat ggaaggcaat aaatgaagga tgtaacatta aggggtatca
     1921 tctttggaca tttattgatt gctggtcatg gatcaacgcc tataaaaacc gatatgggct
     1981 ggtttcactc gaccttacta ctcaaaaaag aacaattaaa aaaagtggcg aattttacaa
     2041 gcaattaagt gatacaaatg gatttagata cgatgtagac ttgttagttg aataatattc
     2101 taaaattcca cttgaagtga acacattaga aaacaagaat aatgagaaat accaataaca
     2161 aatacttttt cttcatactt catttatact tgtcagtata agagactgtg acaaatgtag
     2221 tgatcctcag aagttaggtc tggaaatcta acttgagaag atttttttaa actacgtcca
     2281 gtctttttta tttgtttaaa attgaagttg acaagtcttc attaaaaatt tagaatattc
     2341 acaagtttga cgacaattat gaaaatttat gtcgtgctaa aggagttttg ttattggatg
     2401 aaaagttaga tataaaaact gcgatcaaag acacgttacc taccgttttt ggttatatcg
     2461 gtattggact tgcatttggt atcgttggga aagctgccgg gtttcatccg ctggtcgtca
     2521 cgttgatgtc cctgctgatc tatgctggtt ctgcccaatt tatcacggtc agcatgcttg
     2581 ctagccatag tccactgctc tccatcgttc tctcgacctt tctagtcaat tcccgaatga
     2641 ttctgatgag tatgacgatt gctccttatt tcaagaaaaa tcggctgctt caaaatctgc
     2701 tgattggcac attgctgaca gatgaaagtt tcgcactggg aatgaataaa ttgaactata
     2761 ctggacaaaa gttaaacttt cgttggatga atacagctaa ttggatttct tatttgacgt
     2821 gggtgggttc ttctcttgtt ggcgcacttt tagggaattt tattactgat ccgaaaaagt
     2881 ttggtttgga tttcgccatc gtcgctatgt ttatcgggtt actttatctt caagtcattt
     2941 ctgatagaaa cacctctaaa cgattacagt tgatcctgat tggtctcact ttgattctgg
     3001 tatatgtcgg actgattttt atcccaagta acttagtgat tttagttgta acactgattg
     3061 ggtgcggatt gggggtgtgg atcaaacatg ccttcttttg agtttacatt gctgactatt
     3121 ttaggttgta ctttagcaac ttggctttct aggatcctac catttgtctt actgaaaaaa
     3181 ttcgatttgc cgcaaccgtt gcttgagtat ttaagttttg tcccgatcgt cattatgtct
     3241 gctttatggt tcagcagtct atttacccaa aatatcggac atctgccgca gatcaatctc
     3301 gaaaatgctt tggcttcagt tcctacactt ttagctgcta ttctttcaaa aagcttattg
     3361 gtgatcgtct tggtaggaat tctttccctt tcgttgattc gcttattata aataaaaaaa
     3421 caagagctta tggcaaatta ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa
     3481 aaaatcggcg tattcttgct tatccgttga acggaccact aacaacgact tttcctaata
     3541 tatgtcctgt ttctacgtct ttggttgctt gtttcaagtt cttcgcattc atcccatctg
     3601 aatagacctt gctcaatgta ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg
     3661 ctaaagcttc tccttgcgtt tcgatttcat agttatagtc tgttttagcg aacatatact
     3721 cccagtcgaa gctgatagat tggtttttcc aaacacctat atctaacggc tctttgatcc
     3781 ctacgatcgt tcccacatgt ccaaatggtc ggacaaggtg tttgatttga tcaaagtagc
     3841 gggtaatatc gaataataca gcgacatact ctacttgatc gattcctaat tcacgaaaac
     3901 ttttacttaa gtcgtgatga taatcgattg gatagtctac acctgttttt ttcagccatt
     3961 caaaattctt cggacttgca gtcgcgtaga cttctaggcc agcccaatgc gctagttgat
     4021 tcaaaatcga accgacgccc ccagctccgt tgatcactaa gatttttttc ccgcggttgg
     4081 cattttcttc tggagtcaga ccaaattttt caaataacaa ttcataagcg gtcaatgagg
     4141 tcaacggcaa ggctgcggct tcttcatctg aaagattttt tggtgcaaga gcgacaatac
     4201 gttcgtcaac cagttggtat tcttgattgc ttcctgctct tgtcgttgta cctgcgtaaa
     4261 aaacgcgatc gccgacagaa aatttttgca cttcatcacc tacagcaaca actttcccga
     4321 ctccatcaaa ccctaagata cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg
     4381 tatcgacagg attaacagat actgcgttga cttttaccag aagatcccgt gaactgggta
     4441 caggtatctg ttctttttca tcaataaagc tttttggatc atcgatcgac aacccttcat
     4501 aaaaaccgac taccttcatt aattcctttt ctgacatacg aattcctcct tagcttcatc
     4561 aagttggagc atccatacag ttttcatttc aatatatata gtatgctatt tgttccactt
     4621 ttattcatca taattattct cttacttaaa gtaaactatt tgcacttttt tggttaaaaa
     4681 agaggttgcg acaagacttt tgtcacaacc tcttttaaac ggtgttcaat caactgaccc
     4741 tcggtattcg ctcaaaaaac gacaaaatat gaggagctat ttttgtttgg ctgttcttat
     4801 tacttgatgc aggacagctt tttcacgacc tctaattcac ggtgttcaag caagaaatca
     4861 ttgttcactg aaaagcgttg gatgttcttt ttcaaaggtg tctaagatct ctcgtacttc
     4921 atcagtagat ttcgtattca tcaactgaac tcgcaagtca tttgctcctg gaaatccttt
     4981 gatatagatt ttaaagaaac gatgtagtcc tgtgatggaa cgcgggatga tttctgaata
     5041 atgatcttgc agatctagtt gcagacgaag tagtccgatc agttcttttt ctgtatgttc
     5101 cctcggttct ttttcaaatg cataaggatt cttgaaaatc ccccggccaa tcatgatgcc
     5161 atctacgcca tatttttcgg ccaattcttc tcctgtctgg cgatctggga tatctccgtt
     5221 gatcgtgatc agtgtttgcg gagcgatttg gtcacgtaaa gcaatgattt tagggatgac
     5281 ttcccaatgt gcatctactt tactcatctc tttacgcgta cgcaaatgga cagaaagatt
     5341 ggctatatct tgatgaagta ggtgactgat ccattcttcc atctcagaca tttccgtata
     5401 gccgatacgc gtcttgacgc tgacaggcaa accgccagct ttagccgctt caatcaattg
     5461 tgccgctact tctgggcgaa ggatcaagcc actgcctttt ccacgttctg ctacattagg
     5521 gactgggcag cccatgttga tatcgattcc ttggaagccc atttctgcca tagcaatgct
     5581 catttctcta aaaaattctg gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc
     5641 ttctgtaaag gtcaatcttc ctcgtacact gtcttttcca tctggatgac agaaactgtc
     5701 tgagtttgta aactcagtaa agaagacatc cggtgctgct gcatgcttta cgacatggcg
     5761 aaatacaaca tctgttacat cttccattgg tgctaaaaca aaaaaaggct ttggcagctc
     5821 tttccaaaaa ttagtagtca tttatttgtt actctccatt ctatcaagca gtgaagtcgc
     5881 aatctttctc aaaaacattg tcattatact aggaaacaaa aaaagaagca aagtgaacag
     5941 cttaaaagat gtttctgatt gagcaaaaaa agactgtgac aaaaatctta tcacaattcc
     6001 ttttccggaa taaatggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaaacga
     6061 aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcaga acagcttttt
     6121 cacaacctct ccactaaacg gtgttcaatc agctgaccct cggtattagc tcaacaaacg
     6181 acaaaatatg aggaactatt ttcccgtttg tttctctaat actcaggtca aaacgctgat
     6241 ttcacaacct cttttctaac ttctcaatcc tcttccttta ttgatcaaat accaagtaac
     6301 ggcgtacaaa acaacgatga atagcacaag tacgatcatc gatgttgcta ctggcacatc
     6361 gattgttcca aggaatccgt aacggaaacc ggagatcata taaacgatcg gattgacttt
     6421 cgaaacagct tgccaaaatg gcggcaacat agagatggca tagaaaacgc cacctagata
     6481 agttaacggc tgcaagacaa atgtaggcac gatcgataca tcgtcataag actgagcaaa
     6541 aatcccgttg agcagtcctg ctaacgaaaa caagattgcc gtcatcaata atgtaatgat
     6601 gaccatgaac catgaataca catgaagtgg tacaaagaac aaggaaatga tcgttacgag
     6661 agagccgacg agaatactgc gacccactcc gccaatcaca aagccccaaa tgatcacatg
     6721 cgtaggaaca ggcgctacca agatttcttc gatgtttttc tggaattttt gagagaagaa
     6781 ggacgaagaa acgttggcat acgaactagt gatagcagac atcataatca aaccaggcac
     6841 gatgaattcc atatatgaaa aaccacccat gtcaccgatc cgaccaccga tcatttttcc
     6901 gaaaataaca aagtacaagg atgtcgtgat cacaggcgga accagtgttt ggacccagat
     6961 acgcaaatag cggttcgttt ccttgatagc taaacttttt aatgcagtaa aataaagatt
     7021 aaacattttt ctcctccgta tgattatttt cttctgtgat tttcaaaaac agttcttcta
     7081 aacgattcga cttattcctc atagaaagaa ctttgattcc ttgtcttgtc agctgatcga
     7141 agattccgtt caccccttga tttcgttcaa cttcgacagc gattgtctgt tcatcttcaa
     7201 atgtacaagc gtatccttca atcacaggtt tggtctcata tggcgctaaa tcaaagataa
     7261 atgtttcaaa ttgcagtttg gatagaagct ctttcatact agtattctcg atcagttcac
     7321 ctgattggat gatcccgatg ttgcgacaaa gcatttcagc ttcttctaaa taatgcgtcg
     7381 tcaaaatgat cgttgtacca ttttcgttca attctcggag aaactcccac atctcgcgtc
     7441 tcaattcaat atcgactcct gctgttggtt catccaagat cagcaaacgt ggttcatgca
     7501 tcaatgccct agcaatcatc agtcttcgct tcattccccc ggaaagcata cgtgcacgga
     7561 catttctctt ttcccaaaga ttggactgct tcaaatattt ttcgctgcgt ttcatcgctt
     7621 ctttgcgaga gacaccgtag tagcctgctt gattcacgac gatttgctgg accgtttcaa
     7681 agggattgaa attgaactct tgcgggacta atccaatctg ttgctttgct cggactagat
     7741 ccgtatccag atcataaccg aagacattca cttttccaga tgttttattg actaaagaag
     7801 taatgattcc gatcgttgta gacttcccag ctccatttgg tcctaatagc gcatagaaat
     7861 ctccttcttt cacggaaaga tcgattcctc gcaatgcttc gaccccactt ccataaacct
     7921 ttttcaaatt atttatttca agcgcatttg ccatttattt cctatctcct tatagacatg
     7981 attctttatt gtatcatgga atacagcaga tgaaaaaaca acttggttcc agcgaaaaat
     8041 caggttgctg ggctcaggaa aatctcctaa tgaaagacat caatcggacg aagtcagaca
     8101 accttttcag aaaaaacaaa tcgaagataa aggattacta ttcatatgtg caacaaaagg
     8161 agcgtgagac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     8221 aagcaaaagc caagattttt gatccaataa atactttcga tcacttcgcc tatcccaata
     8281 catgccactg ccgcttttca tcaaataacg cggcttccgt ttttgcggtt tctactaaag
     8341 tctctgggta atccaaaact ttcagcaagg caatcgcatt tcgggtcctt gaaggtcctt
     8401 cttttaattt gaaatcaaaa ctgattcctt ttccttctgt tacttgttct tcaaagtgga
     8461 cattctcaca ataattcttt aaaatctctg tcaattcgat atcatgtgtg gcgacaaatg
     8521 ctaaagatgg atactccgca agccaccgaa cgactgaagc ggaagaagca atccgttcga
     8581 tcgtatttgt cccttttaaa atctcatcta cgaagcagta gcagcgttct ttggaagcca
     8641 cttggtccaa caaacgtttg atcgatttga tttctgaaac aaaataactg tcgccttcaa
     8701 ataaatcatc ttcgattgcc atggaagtca acacatgtcc aggttgcagg gtaaactgtt
     8761 ctgcaatcgc tgtttggatc gtctgtgcta gaatacagct gatggctata cttttgacat
     8821 acgtagattt tcctgatgca ttggaacccg tgactaatgt attcttctgc cagttgaccg
     8881 gattcactac cgcttctttt aacaatggat gatagatatc tttcgctgtc acaccgccct
     8941 cagtaaactc tggtcggcaa gtgatgggca tataagtacg gtaattcaat attgcagcgg
     9001 ctacttctaa ttctcccagc aattcccaca gcttgatggc ttcagttgaa tgcttttcta
     9061 atttagtgag aacgaaatga taggagataa atggcagcat gaacatgatg ttgacatatt
     9121 caaaaatcaa ctctgcttca ctcccactct tcatacgaaa tgaaaaacct aatttaggga
     9181 tgtctcttaa tggtttagtt tgctgtttca gctcttcttg aagtggtgtc ggcagttttg
     9241 ctagctgatt ggcggctgaa atcgttgaaa ccaaataact catgctattc agctctgttt
     9301 ctagtgtttg tttcttcact agataataga ccgtattgaa tactaaccca ccgattgcca
     9361 aatagattcc tagtacttgt cctaaaagaa gcaaacccaa acccaccagc ggaagcaagc
     9421 caagaaaaat aaataaacct acgcgtccaa tctttttact ttttccatcc gctagatatt
     9481 gttttgcaaa attatgatct ttttttccta aacgagcgaa ttgatattgg attttttctc
     9541 tcagttgagg attttcctga taaaaagcaa tcagtttttc taactgctga tcttcaccaa
     9601 aatcaaagct gcgtaaacgt tgatacaacg cttctgaacc tacactagaa taggtactgt
     9661 tgattccttc gaatacttcg aacatatcca ggtcatacca agtaagatca tcgatttcgc
     9721 tatcccatga acggaacttc ttctccgtga gccatgcttc cttcaagctt tcttctttat
     9781 caaatctcgg ttgataagga attttttccc atttgctacg aactgtttcc tttaatttca
     9841 aacgtgaaaa aatgtctaaa gcaactaata aaataacagc tgcaataata attaaaacga
     9901 taaattctct ttgacccata tccctctccg ttcctttatt tgttagcttt agtatagcac
     9961 tgttgcgcta tttgaaaaga gggtaaaaaa acacctttca aaataaaagg tgtttttttc
    10021 ttgtatatta catatttatc tctcatttat caaaattgac aaataatcat agggaggaat
    10081 tgttgttaat tcttgtttat tctccagtgc attgacaact gtcccaaggg aaaggatagc
    10141 ctcaggtact attttttctt gttctgttgg attagctaag cgaagaacaa atccttgttc
    10201 atgaaaataa gaaggataaa tcgatgatac aattaagtct tcaggtaatt caatcaatga
    10261 aaatttacga ggaacatcaa gttcattttg taatggccaa atcttgttat ctaacctgtg
    10321 aataaataca ttcagtttct gcttttgata agaaatagat tgttctaacc gtttttctgc
    10381 ctgttttgcc actttgaact cattaaatct tccttcttct acattaaatc caaaagtgac
    10441 tttatactca cctatctctt gagctagcgg tgtttccatc atgacatgcc cttcatttgt
    10501 cgtatctcct gaagctcgcc caggtcgcca agcaagatta ggttttccaa gttcccctgt
    10561 tgttgaaaat aaggttatga ttaattgatt ttctatacgt tcatactctt ttaaaccatc
    10621 tgcataaaac gtaaaataat gatcattttc tgccacagag acactcttat cgaatatttc
    10681 taaattaaca ggtttctcta ccatagtttc tgaccaattc ggaggaatga catcaggggt
    10741 attttcaata aatccgtttt gaatttgtgc aattgatttt tcagatttat ctaaaacatt
    10801 aattgctaag cgcatacgat gcgagtaaac agtattatga attttgagtg tgccttcaac
    10861 gatttgactt tctcccagac tgaacgatat ttcataagat atttcttcca tttcgggttt
    10921 ttcagataaa cgatctttta aatctttagg aagttgagct tttccgtaaa caactaatgt
    10981 ttcttgtaaa gaatctttat atactttagc tgtttctaat tttagagata gttctgtatc
    11041 tccttctaat ggtgaataat catacgtatc gccatcattt gctgaatcga tcaaatgaac
    11101 gaaatcaaga tattgtctat ctccaactat caaattcaat tgcccatctt taaaagaaag
    11161 tttatattta ttgttactta tctcagtttt gtctatctcg attgctgttt ctaatttttc
    11221 attatttttc tcaaattcaa caacagtgta tcctagtgct ggcacattta catttatttt
    11281 tatgtcaagt tcataataag ctggttcatc aaaatattcg aaacctgtag caatcatccg
    11341 ataagcattt ttccgttctg gaaaataaac ttctttttca atgatattat agggactacc
    11401 tttgaagtgt attttctttg aatcagctac tacatgcacg attttcgatc cactataatt
    11461 ctcagtcgtt gtgttgaaaa caaggacttc attcgatttt agctttaatg catcagccat
    11521 ccgttttaca ataaggtttt cgattccgtc agctatctca ttagcttctt tgattcgatg
    11581 gaaaatatct tccgcaacat tatctgaaac acagccacca atactatcat gagcctggtt
    11641 ttgcaaaatc tttttccata accgacgaag taggccttta ctgatatgga taccatttct
    11701 ttctgcaatc agcatgagcg gctcaactcg ttttagaatt ttttgttcca atttaaaatt
    11761 ttctattttc atttttgttc ttactgagcc aatagaacga tgaacacgag agtaaaccgg
    11821 ctgacgaagt tcacctttat attcaggtaa ttcttttttg tttctgataa cttctacata
    11881 agaaggataa tctgaaataa tattgtggaa tttactgtat ttattcaact tctctacgac
    11941 ttgatcaaaa tttaaaatca ttgacttttg atctacaccc gaaggtacta aaatatcttt
    12001 gttttttcct tgcatatgaa gcatttcaac tgcttcatcc aacttttctt ttgcaaattc
    12061 aggaatatct ttaattgcgt ctaaggtcat cattccagtc atataaccaa atggaaaatt
    12121 catggcataa acaaatttat ctcccaatcc tttccagata aaataaggag atggaaccat
    12181 cgtttcaaag tttaatcctc gccaacacat gaaattatcg atttctactt gatgcaaaag
    12241 tgtaggcaaa ttcgcattga acccaaacgt gtcaggcaaa taccctacca tcattggatc
    12301 tccatattta aggatcgtat cactgattcc aatcattaag tttcttaaaa ttgattcagc
    12361 atccactaat aaggcatctg tttgtgcata ccacggtccc acgaatagtt gtcctgtttt
    12421 catcagtcgt tggataacct taagcttttg cggattgatc tccaaatact catcgacgat
    12481 tgaagtttgt ccatctaaac aaaagtttac tttagggttc ctttccaatt catctagaac
    12541 ttcagtaaag acctgatcag acaatacgat ggcatcttca tttgtaaaat accattctct
    12601 gtcccagtgc gtgtgttgaa ttatatgtgc atttaccata tcagattctc ctactttcct
    12661 tcttctactg aatttacttg ttgttctttt ttatacttga tggattttag tgcattgacc
    12721 attaacgctg taaccactcc cccaataaca attgatacta aatataaagg tatattcgta
    12781 acaaaaggaa ttgccataaa ggttgaaagc attgtttctt gagtaacacc aaaaaatcca
    12841 tttagaccac atgctactgc agtgcccacc acgatgcttg gtattactcg taaaggatct
    12901 ttcacggcaa acgggatcgt cccttcagta atccccacaa ggcccataat catagctgtc
    12961 tttgcattat ctcgttccga ttcatcataa agttttttgt ttaaaattgt tgctaaaaac
    13021 attccaaatg gcggaatagc aattcctaat aacatggctg tactaggtcc aaagcttccg
    13081 cttgaaaaag aagcaacccc aaaagcatat gcgattttat tgataggtcc tcccatatca
    13141 aacgccatca ttcctcctaa aatagccgac atcaaagcag catttgctcc tgacatgcct
    13201 tctagccaat tagtcagtcc tgttgtcatt gcttgaatag gcgtagctag tacataccat
    13261 aatatgattc caacaactaa tgtagtaaca aaaggaatca gcataattga ttttagacta
    13321 ataacttgca tcggtacttt tacttttttc aatgcattag ctaaatagcc acaagctaat
    13381 ccagcaacca ttcctccaat aaatcccata ccaagatttt gacacatcat cccagtgata
    13441 aaagctggtg ctagaccggg acggtcagca attgaatatg cgatataggc tgaaatcatc
    13501 ggtacgataa actgtaatcc cgtctgacca atcattcgca ggactcccca gaatgtatcc
    13561 gtctggtccc aaacacctgt gccaccaaaa atgactgcaa tagaagttag taaccctgct
    13621 gctacgacaa gcgggatcat ataggaaacc ccactttgga aatgatttag aatttctctt
    13681 ccttgtttct tgaattccat tagtaactcc cccacgatat attttttatt tgttaattaa
    13741 tgcaattgct tcatcaatcg tctcttcacc atcttttact acctctgagg tccctactgt
    13801 taaaatcggt ttaccgtcaa agcgacctcg ttctgaaaca tttgtatcta ctgcaaatat
    13861 gacaacatct gcctcggaaa catccgaaat tgaaagtttg ttttctatcc ccatagcccc
    13921 ctgtgtttct actttaatat ctaacccttt cttcttcgca aacttttcta aattagcttt
    13981 tgccatataa gtatgggcaa tccctgcgat acatgctgtt actgctacta tttttgtcat
    14041 atgaaattcc tccttttaaa atgatagagc ttttaccaat tcttttttat ttctcgaatc
    14101 ttttattctt ttaacaaact cgtcatccat caatcttcca gataattctg ctaaaatacg
    14161 taaatgatta gtacctttat cagtgtcttt tattgctaac aaaaagacga gatcaactgg
    14221 ttgatcatct aatgcgcccc attcaacaga ccgctttaac ttggctacag ccactgttga
    14281 ttcaattact gctaggcttt tgccatgagg aatggcgata ttttccccaa ttcctgtggt
    14341 catatggttt tctctgtcaa atacgtcttt ttgaaattgt ttgtggtcag acacaaaccc
    14401 attagtctct aacaatttcg ataatttttc gactacactt tttttatcag atgcatttaa
    14461 atctatcact atattattta tattgatttt ttcactgatc atttataagc cttctttcaa
    14521 tatataaact aatttttcaa acgacgaact cgtttttaaa tttgctaata actcatgatt
    14581 cgaagcgatt tcattgaatg tttcataaat tttattcaat tcaggtttta ctttttcatt
    14641 aagtgcaaaa aagaaaacag aatgaacaag tgtcccatcc caatcaattc cttctggatt
    14701 aacgtagaca cagattactg gcttttttat aaaatctact tcgatatgcg gcatagcaaa
    14761 atcattcatc gctgttgaag atagtttttc tcttttgatt gctccctcta gtatcccttc
    14821 tttcgcgtaa ttttctttta ccaatttttt ggtaattata tttaaaacgt tcacttgatt
    14881 atcctttatt tggtgggtaa aaaagcaaaa tcgtttatct aataaattta gaaaagcatc
    14941 ttctgtttgt tcatttaact gtagatactg ttttactttt ctgacatctg cccctaacat
    15001 caaaggacta acaacaataa caggaaaatt tgtattttca atcggaatcg tgcttataac
    15061 aagttcatca gtgacttttc tatcttgtag ttcattgatg cttagtacat cttctatctc
    15121 aaccatatct gcaaaaccgt tatttattct ttgctctaat aattttgatg ttccatatcc
    15181 gctactacaa actaatatca ctttcgtttt gtcaggtttg taacgatcca aaagtgattg
    15241 aatatggagt gttacgtaag cgatttcatc ttcattaaaa tgtatacaga atctctcttc
    15301 gatttcctct aataaatcta cacttatcat aaatgacctt ttaaaagaat atttgatttt
    15361 gtccgtataa gggttataga tatttactcc caatttgagt cttttaactg ctgcattgag
    15421 atgtaatact agattattga tcaattcttc atcaggataa aattcattta atttaattct
    15481 tactaactct tgtagctcaa cacccatatc taaataggaa agcttcgttt gatctaagtc
    15541 gtgattcata gcattttttt caatcgaagt aatgtgaatg tcaacataag cttgttcatc
    15601 tattggcaca ataattgaaa atgctttttc aatcattttg attaaaataa gtgtattctt
    15661 tgagatttct tcttcagaat tattactacg tgataagaaa ttgcttgttt caatcctttc
    15721 tatcgcaata gcaaggtgga tagctaaagt ttgaaattca taatctgtaa atttcaattg
    15781 tgtcttattc atgtatcgat taagcaattc ggctactttt ttcaatatgt ccttatttaa
    15841 gaaattattc gcttcaccaa tgaaattgat attcaagtct aagagatctg ttttactgtt
    15901 agatgctgta aagccacccc aataatagtt cacaacttct gacatcaacc ttcttttact
    15961 tttttcatca gctttgatgc gcacgccatt tcgatctgaa actaaggaca tcccatgctt
    16021 gtttagtctt tttctaacat ctttcattgt gctttctacc gtcgacctgc tcacgtataa
    16081 ttcgtcagca agattttgaa tctttagaga atcattagta ttcaataatt tagcaaacac
    16141 ataagttaga cggtcctccg gagagtctgc gatattgatt ttatgactgg ataataaatt
    16201 tcgaagtgtt tctttttttc caataagcga aacgcctttt ttaggttgaa ccaacatttg
    16261 tacatctggc attttttgga ttatttcttt caaaattaaa atatctttgc taattgtttt
    16321 cgtagataca tataattgtt ctgataattc tttgtaagtt gtgtgctcat tttcaatcaa
    16381 aaagtatagt attttcattt gccgttcgct aaattcgatg cccattatct tcacctcctg
    16441 aatacagctt aagtgtacaa ttattatctc tattttcata cagaagaaag ttcttgatta
    16501 atgtagaagt ttctaatatt cttgattatt tcaaatcaat aaaaaagcct gcaatattta
    16561 tatcacaggc ttttttctct attaaatata tataagataa tacatttttc tactagttct
    16621 cactaatctt gtataattaa accctccgca acttgacgga gatggagaag atgcccccgt
    16681 ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac aggtaaagcc
    16741 cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa cgctggaaga
    16801 ctttttcaac agaggtttgc gttcctggtt tgataggatt ggccactgtt aatcgaatga
    16861 atggatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc aataaattct
    16921 gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca gttgtattta
    16981 tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca taaaaagaga
    17041 aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt tctttttgct
    17101 tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat cttgcaacag
    17161 attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc tgttccagta
    17221 attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa aggccgacga
    17281 tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt tcattttcat
    17341 ccaaccatgt attcagtgca tctactaggt ccattagatt cttttctcga aattcgactg
    17401 aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta gttaatctct
    17461 tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg agaagcaaca
    17521 gccatttaat caaatctaaa ccccactccc cacaccgtat gaatatacac agcatgtgcc
    17581 tttgctagct tccctcgaag attggaaata tgcatattca ctgttttctc actatcaaaa
    17641 tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt tttcggatgt
    17701 tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc cacaccattc
    17761 acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat gcgttgcgac
    17821 agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg taacaaaagt
    17881 tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa caaggaaact
    17941 ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga tgtctgtcgg
    18001 atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag aaggatcaaa
    18061 tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc atcaagaagt
    18121 ttgtatctct gttcaagaac ctcctccagt aaggaatgaa cagcttcatc gtcttcgata
    18181 atcaaaatag tttccagcag gaccacctac ttaatgtcag atttttggaa aataaacatt
    18241 cctagagctg atgataccac gattgttact agactgacca gaatcatttt cggtgcttgc
    18301 gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt aaaccactcc
    18361 gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc cgttcccatg
    18421 atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa tgtcaaagcc
    18481 actggaacag ataatgtccc taatgtcagc agaagcaata caggaaatac ttttagaaca
    18541 taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag tgtgtaagca
    18601 agcatggcta gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag cgtaacgaga
    18661 agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt gcgaatcgta
    18721 ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc cagaagaatc
    18781 catgcagcca ttttttctac cattaaaatc cctgttgcac cgttgatgac tgtatccgtt
    18841 gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca agaataaaaa
    18901 agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt ccatactttt
    18961 attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt gtaagaaata
    19021 atcttccagt ttttgcccaa ctaggtgaat accatccaca ggaacttttg cttctaccaa
    19081 tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa cacggctaga
    19141 ttgtacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt gttccgtcgc
    19201 cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt tcgataattc
    19261 tttgatcaag tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg acaattcatc
    19321 tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac gttggatgat
    19381 ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat ccaaaatcaa
    19441 aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca tacctaaaga
    19501 gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca gcaggtcttc
    19561 gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat ctcttgcagt
    19621 taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc ttgctgcctg
    19681 caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca aacccataat
    19741 agttctcata aaggtagact tacccgctcc attttcccca atcaatccat agatcattcc
    19801 acgtttgatt tcgattgata ctttatctaa tgctttggca gcaccgtatt ttttggttat
    19861 accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc tttcttattg
    19921 atgagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata aagaaagtgt
    19981 aaattttcca attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt cctattatca
    20041 ggcagtttgt tatacgcttg tatcagtttc ctgattcttt cttttcgacc aaagtcgttt
    20101 ctctgtattc ctcttcactg aaaatttttg cttctttcag atcaagcttg agtaatttta
    20161 ccagaccggt tataaggata actatagcaa gactccctcc cagaaaaatt agactgtctc
    20221 gcagacgtgg ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct aatccaagtg
    20281 gactggccac catggtaatc gttgttatcg ttgacataag tcgtccttgt atatgcgttg
    20341 gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat ccttaaaaac
    20401 ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca aataagaggt
    20461 tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc ctgccaactc
    20521 ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa attccttctt
    20581 atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa tattgtttga
    20641 tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga ccaagaatcc
    20701 tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg aaagtgccag
    20761 aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga ttctcgtaac
    20821 tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct ttacgggctt
    20881 tcttaagacc tggttttttc ttcgtatcat tgaataagtt tgaaaaacct tgatttataa
    20941 aggttttccc gtaaatatat tttattatat ttgattaaat ttgaaatgtt tgtgttctaa
    21001 aatgtgttct aatttaaagg cagtatattt cgtattttag attaaatttt taggctcatt
    21061 cattctcaaa tccattctat tcagtcaata aaaattttaa aaagacttta taaacaaaga
    21121 tgttcaagcg agcatcttat ttttttgtgc ttaatttgaa aggagaacat ttcatgtata
    21181 aaaaacaaaa aaagaaatta tgtgtattaa ctattttgag tttggctgtt caagcaattg
    21241 ttggagcaat tgctcctacg attacttttg ctgatgaaat tacccatcca caaacggtaa
    21301 ccgtagaact agatttagca catcaatatg ctgttgaagg aacttttagt gatggtcgtc
    21361 caatgtcaga agtcactgtt ccgcattatg cagtgtacaa cggctttaaa caagatattt
    21421 tctgtattga accaggagtc ccgatttaca atgagttcac tcctggctat gagaaaaacc
    21481 cgttaccaga tatgtccgat aaagccaaat tagtttccgt tttatggaaa aaagtaggta
    21541 cagatgcaga tactcatatt gtggcacaga agatgatctg gcaagaagtt aatgggtata
    21601 cccttcactc aataaaacga tcagatggta gtgccgtaaa tatcgcagcc attgaggcaa
    21661 aaataaatca agccattgac gattatcaga agaaaccaac cttccataat agtacggcta
    21721 aaacagtatt agggcaatcg actactgtga cggatacgaa taatctgaat ttgtcagagt
    21781 tcgatgaggt tgtggaaaat gccgcaaaca ttgattatcg tgtgaatggt aatcagttga
    21841 taatcacccc aaatgccaat tcaaaagaaa gtggtgtgct gacgcttaag aaatctgctg
    21901 gtaccggaac acctgtcgcc tataaaaagg ctggccaaca gactttaatg gccggggcga
    21961 ttgataaacc taatacctac acaatcaaaa ttgatgtgga aacggagggt tctttaaaga
    22021 tcaaaaaagt cgataaagaa tcgggtgatg ttgtaccagg aacggttttc catttagact
    22081 ttggaaaaac tttacctgca aaagacgtga ctactgacaa agaaggcatt gctacattgg
    22141 atgagattcc tcatggtaca aaagtaacca ttaccgaaaa atcagtaccg gcgccttata
    22201 cgattgatac cacccccatg actaccacca ttaaagcagg cgaaaccatt tacgtgactt
    22261 caaaaaacgc acgggaaaaa gggcaaatca ttctggataa aagcggagtt gaaacaggga
    22321 gtgatctttg gaatgacaat tattccttag ctggtaatac gtttgccatt cgcaaagaca
    22381 gtccaaccgg tgaaatcgtt caagaaatga ccacggatga aaacggtcat gcggaaacac
    22441 caaaagagat tgctaatgcg ttggaattgg gaacctatta tgtgaccgaa actaaagcta
    22501 gtcatgggtt cgtgaatacg ttcaaacctg taaaagtcga attgaagtat gccaatcaaa
    22561 ccgtagcccc tgttaccagt aacgtaaaag ggcaaaatca agaagtcact ggcgaaacca
    22621 ctttgacgaa agaagacaaa gatactgggg ataaagcaca aggtaaggca gtctttgagg
    22681 gtgctgaata caccttgttt actgctaagg atggaaaagc tgttaaatgg agtgaggctt
    22741 ttaaaccaga gttagcgaag ggaacgaaag gttctgatga aacggtcact ttgactttag
    22801 acgaaaagaa ccaagttgcc gttaaacatt tagctatcaa cgagtattat tggcaagaaa
    22861 caaaagcgcc cgaaggatat agccttgatg aaacaaagta tcctgtatcc atcaaaaagg
    22921 tggatgatag cgaaaaaaat gccgtgatta cccgaaatgt tacggcaaaa gaacaagtga
    22981 ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgcgggcact accgaaactg
    23041 gcttcaatga tctgaccttt aaagtgtcgc cattggaagg gaccaatgaa atcacaggtg
    23101 ccgaagatga agcgaccaca gcttataacg agcaattagg gtttgatggt tatggcaagt
    23161 ttgaaaatct tccttatggg gattatttac ttgaagaagt agaagcccca gaaggttttc
    23221 aaaagattac gccattagaa atccgttcta cttttaagga aaacaaagaa gactttgtga
    23281 agagtgagta tgtctttacc attaccgaac aagaccaaaa acaaccaatt aaaacggtga
    23341 ccgttcctta cgagaagctg acgaacaaag cattttctgt tagtttgaac cgtttaatgc
    23401 tctatgattt gcctgaggaa gaagatagtt taacttctct tgcgacttgg aaagacggaa
    23461 acaaagaatt aacttctctg gattctaccg agctagttga taaattgagt tacaatctac
    23521 atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggat gcgacaaaag
    23581 ctgcccaaga aaaagatgaa aaagccaaac cagtggtgat tgcagaaaca agcgcaacgt
    23641 tggcgaacaa agagaaaaca ggaacttgga aaattcagca taaattaacc gccgaacaag
    23701 ttttaaataa aactattgtc ttattcaact atgtgtatga aaataaggaa gcttttgaag
    23761 caggcgataa gccagtagcg aaggatgtta gcttgaataa ccaagcccaa actgtcagtt
    23821 gtacagtgga acaccatgtt tctatccaaa caaaggccca cctagaaaac ggttctcaga
    23881 cctttactca tggtgatgtg gtggatatgt ttgatgatgt gtcgattact catgatgtac
    23941 tggatggctc aaaagaagct ttcgaaacaa ttctgtatgc tttattaccg gatggtacaa
    24001 acaaagaaat ttggaaatct ggcaagattg attatgaagt aaatgataaa gaattcacca
    24061 aaactgtgct tgcgaaaaaa gtagataccg gaaaatatcc agaaggaacc aagtttactt
    24121 ttgcggaaat caattacgat aaagatggga atatcaatgg aaaacacaat gaagatttga
    24181 aagaaaaatc tcaaacctta acaccaaaag aagttccaac catactaagt acgccgaaac
    24241 aaccggaaac accaactgtt ccaagtggct ctcaagaatc tagtcccaca gtgaagacct
    24301 tccctcaaac cggggagaaa aattccaatg ttttactgtt cattggtttt actttgattt
    24361 ttgcgacggc gggctattat ttctggaatc gccggaacta aggtgatgag atgatacaag
    24421 taaatcctaa gaaccaacgt gcgcgaccgc ctcctaaaaa agtagtcgcg ctatttttgt
    24481 atccaaaact aacaatgaaa agaggaaaag aagatggaat tgaaatttgt cgtgccagat
    24541 atggctgaaa cctttggaaa gttaagttat gccggtgaag gagaaattat aacagaagga
    24601 tatggacgga atactacggt gattggtcgc agttatcatt tgtattccag taagcaacgg
    24661 gccgatgata ttgaggtggt agtagctgcg gaagccggtg aaaaggattt tgatcaagac
    24721 caaccgctaa aagccgtgaa tccccatttg gttgccaaag gctatgagat tgagaatcgt
    24781 gggtttactg attacgtgtt gtatgttgat gatttagtga aagcatagga ggaaaagaag
    24841 atgagattaa cagaaggtat tgtcgtagat tcaggtttaa catttgggaa gttgcgtttt
    24901 tccgcattac gccgagaagt acggaagcaa aatgaggatg gaacaattgg taatgaggta
    24961 aaagaacgaa cctataattt aaaatcctct gcacaagggc gaatgattca agttagtatc
    25021 ccagctaatg tacccttacg cgagtttgct tatgatgcag aggtagagtt ggtcaatcca
    25081 atcgtggata ccgttgccaa ttatgttttt cgagaaggaa ctaccgttaa ttggttcatt
    25141 aaggcggatg atttagtctt gaagcggcag ccgaatcaag gaaatcctac gaaccaaaat
    25201 gaaagaaaga aataggtgaa gattatggaa attaatgttg aacatatttt agattgtctc
    25261 gatcaatatg gaaaaggcga gctgactgag gaacaactcg ttaaagcatt aacctctgat
    25321 gaaaaaatgt ttctgattat acatcaaggt ttacttggag aaaatagcga cagtgaagaa
    25381 gactctgatg tactagactg gttaggagaa gaagagtctt tctttatagt tgtcgaagta
    25441 aatgaaaact tatgtcggca ggcagaatcc attttggaag aaattggtgt ggagatgccg
    25501 gatgctattg aaggcttttt aaatcaattg gttgaaacga aacaactccc ctcctcgtag
    25561 cagtaaatga ctagcataac tttttgaaat tgcctaatga aatcctattg ggctttttcg
    25621 attggaggtg gaaaccatca aaatgtacaa aggacatcgc atacgcgcag gagatcagca
    25681 cttggtttat cactttgttc ttggctggct actcgctctg ttcatcggtt ggatgagcgt
    25741 cttttatttt caagagttta gacaatttga tatttctaag ctatcacttt ctactattga
    25801 aatcgtttgg tctataaaag atttagtatg tctgcttgga agtctcgctt tttcaggagc
    25861 tatgatattg ctttatattc acttttttct tgatcattgg cgaagcttgt ggcatcgaca
    25921 aaagctagct cggatgattc tagagaacca ttggtatgaa gtgaaacaaa ctcaaagtga
    25981 aggctttttc aaagatttga atagtagccg aaccagagag actatcagtt acttccccaa
    26041 aatctgttat cgaatgaaag atggattact gtctattcgc gttcaaattt cacttgggaa
    26101 ataccaagat cagcttttaa aattggaaaa gaagttagaa agcggactgt attgtgagtt
    26161 agtagaaaag gaactcaaag actcctatgt ggagtatact ttactgtatg atatgatcgc
    26221 caatcgaatt gggatagacg aagtagtggc agaaaatggg actctgcgat tgatgaaaaa
    26281 tcaagtatgg gcttatgatt ctttacccca tatgttaatc gctggtggca cgggtggagg
    26341 gaagacctat ttcctcctca ccattatcga agcactattg aagtcagatg cagaattgtt
    26401 tatcctcgat cccaaaaatg cagatttagc tgatttaggt acggtgatgc ctcatgttta
    26461 ttcacaaaag gaagaaattt ctgcttgtgt ggaagatttc tatgaacgta tgatggctcg
    26521 tagcaaggca atgaaagaaa tgtctaacta caaaacagga gagaattatg cctatcttgg
    26581 acttccaccc aactttttaa tctttgatga atacgtggct tatatggaga tgttaacgac
    26641 gaaagaaagt gcggtgattt tgaataagct caaacaaatc gtgatgttgg gtcgtcaatc
    26701 tggtttcttt ctgattttgg cttgccaaag gccggatgcg aaatatttag gagacggaat
    26761 ccgcgatcaa ttcaactttc gcgtggcttt gggtcgtatg agtgaactcg gatattccat
    26821 gatgtttggg gaggtcgaaa aaaacttctt tatgaagcac atcaaaggtc gaggttatgt
    26881 ggatacaggt ggcagtgtga ttagtgagtt ttatacgcca cttgtaccaa aagggtatga
    26941 tttcttagat tcaattagta aattagaaaa gatgaatgat tgagtgatct tcaatactga
    27001 ctttatactt caagtaacgt ataatacttt tatatagtag gaagaaaggg gataattaat
    27061 atggaaagta atgattctta tttagagtat gcaaaagcaa ataaggataa gtttatttct
    27121 tatattatca aagggaaatc ccccgaagtt ggacgagacg cagtttttat ggcagggagt
    27181 cctggggctg gaaaatcaga ggttgccatt ggattagctg agaattatga taaccatgtg
    27241 attattgatg cggattactt tcgcacccag tttcctgatt ataatggaaa aaactctagt
    27301 ctctttcaaa aagcatcttc ttggttggta gagcaggcat ttagatatgt cttagatcat
    27361 ggctattctt ttattctgga tgcaactttt gcaatattaa gtgctgaaaa aaatatctcg
    27421 cgggcgttga aaaaacattt tcgagtgacg attttctatg tttatcaaga ccctaaaatc
    27481 gcttggaaat ttacacaaga acgtgaaatt gcagagggac gtcatgtgcc gaaaaaggca
    27541 tttattaacg cttttttcaa atcaagagag aacattgcaa aagtaaagag taaacatcca
    27601 gatgtattgc ttcatatcat aataaaagat tatacgaata atatttccga agttcattat
    27661 gctacggata atctagaatt atttttacca ctagggtata gtaggaaaga tttggaggtt
    27721 caattacatg actgagaaag aacaagtaac gaaaattgta aagaaataca ataagagcat
    27781 tgcggattta tcggaaaatg ctactgcaaa agagttcaaa acggtgatta aatatgtagc
    27841 cgatcaggcc aatgaaaaac aacggaaatt agttggatta aataaaaaat agtaggacaa
    27901 attaataatg tatgagccaa gagcaaaatg ttcttggctt ttttctttgc aatgaatgag
    27961 gtaatgtatg agatttttag atttattcgc cggtattggt ggctttcgtt taggtatgga
    28021 acaggcgagc catcattgta tcggtttttg tgaaattgat aaatttgcta gacgtagtta
    28081 taaagcaatt catgatacaa gtaaggaggt ggaaatgcat gacatcacaa gtgtatcaga
    28141 tgagtttatt caatccctcg gaccagtgga catcctttgc ggcggatttc cgtgccaagc
    28201 tttttcaatt gcgggaaagc ggcaaagatt ttccgatact cgaggtactt tattctttga
    28261 aatcgctcgg ttcaccactc ttctccgacc taagctttta ttccttgaga acgtccgagg
    28321 attgctcaat cacgaaggag gggctacgtt cgagacgatc ctccgaacgc tggatggatt
    28381 ggggtatgac gtggagtggt aggtgcttaa ctcaaaggcc tacattcccc aaagccggga
    28441 gcggatattc cttatcggac attctcgaga cgcatgtacc gaacaagtat ttcctatcat
    28501 tggaccgtct tcaacatctg atcaaaacat cagaaatttg gtaaatatca atccttccaa
    28561 tcgaggaatg ggtggacaag tctatggttc agatggcgta gcgccaacct taacaggtga
    28621 tgaaggaatc aaaatcgcac tgccagcaag ggatggaatc tctgtagcag gactattacc
    28681 gggaaagttt gaacaaggta atcgagttta tgaagttact ggaaccgccc ccaccttgtc
    28741 aacgaaacaa ggtggtacaa aaatcatgat tcgaaaaaat gaaacagaat atcaagaggt
    28801 gaagccagaa gacagtgtga atctggcttt tcctaattcc acaagtcgcc gaggtcgtct
    28861 gggaaaacag agtgttcata ccttactaac aggagaccaa caagcgatcg tcacggatca
    28921 gtatcaaatt cggaagctaa cacctagaga gtgttggcga cttcagggct ttcccgattg
    28981 ggcttttgat cgagattctc aagtcaattc agatagtcag ttgtataagc aagccggaaa
    29041 ctctgtaaca gttccggtca tttttgatat tgccagaaga ttaaaagagt tggatgtaca
    29101 tggtttatga tgagttgatt ggagaaattt attgggtgat tggaaagatt cagtcagacc
    29161 cagaattaga agaacaattg cgacggttaa aatttgatat tcgcaagaat ggtgtcaaag
    29221 tcgctggcga tccatattta atgaatgagg agacggatgc tcgaattgaa ataaatcaag
    29281 taattgcaga atttgagcgg attgcggacc aagcaaaaga accagatatt cgtcaatact
    29341 tgttagaaat gaaagcagag ttggaagtaa atggtattac agatgagtaa cctaaaccaa
    29401 cacaactgac tgttaggcgc tgaccgcgga ggattcgcga agcagaaatc ctccgtggtg
    29461 ctgtgcctgt tcatggacgg cgttagccga ccatgatgtc tcacccccgt atctaacagg
    29521 ggggtacaaa aacacaaaaa ataagtaagc aaccattaag aatgcttgtt gtatcaaaga
    29581 tagtaagtaa tttgtatagg tgttaactca tttgaaaaag ttaacacctt gaatttggag
    29641 gtgagaactt ggcacaacgg aatttagatt atcggctatt aaaagaccga cgcaacgaat
    29701 acggcgtttc acaaaataaa ttggctacgg cttgtggact tagtcggccg tacttaaatc
    29761 agatagaaaa tggtggcgta accgcatcta taaaaaccat gaggaaaatt tttgaccaac
    29821 tggaaagctt taatcccgat ttgcctttga cgttactgtt tgactatgta aggattcgct
    29881 ttcccacaac ggatgcacga aaaattattc aagaaattct ccatttgaaa tttgagtata
    29941 tgctccatga agattatgcc ttttactcct atcaagaaca gtatgtcatg ggtgatattg
    30001 tggtgatgtt gtctcatgaa gaagataaag gtgtgctttt agaattaaag gggcgaggtt
    30061 gccgacaatt tgaaactttt ttactcgctc aaaagcgtag ctggtacgac tttttcgagg
    30121 attgtctaaa ggccggtggc gtgatgaaac gcttggactt ggcaatcaat gatcgagtgg
    30181 ggctattgga tatacctgag ctaacgaaaa aatgtcagaa ggaagaatgt atctccttgt
    30241 ttcgtacctt taaaagttat cgttccgggg aacttttaaa ggctgatgaa aaggacggca
    30301 tgggcaatac cttatatatc ggcagtctca agagtgaggt ttatttctgt ttgtatgaaa
    30361 aagactatga acaatatatc aagttaggga ttccattaga ccaaactgag acgaaaaatc
    30421 gttttgagat tcgactgaaa aatgaccgtg cttttcatgc gattcaagat ttgttaaaag
    30481 gtcgtagcat tgagagcacc acgttttcca ttatcaatcg ttacttgcga tttgcggata
    30541 aagtagaagg taaaaggcga accaactggc ccttgaatga acaatggggg cgttttattg
    30601 gacggaatcg gaaagagatt caattaactt ccgagccaaa accgtatacc attgaacgaa
    30661 ccttgaattg gctaggccga caagtcgccc ctacgtggaa aatggctaag gagttggatc
    30721 gattgaatcg gacaacctat atccaggata tggtacagaa tgcacgctta tcagaccgac
    30781 ataaaaagat tttggagcaa caaagtatgg caattgaaaa tttgattata tgaaaagagg
    30841 aatcgtcatg ggaattgtaa aagatatttt tttggtattt ggtggcagta tgcttggtgt
    30901 tactgtgatg tgcttgatga atgcaagtgc agcagcggat cgagcaatgg aaaagaaagg
    30961 aaagtgacaa gatgaatttc ggacaaaatt tatgtgcgac tcgttcctta gtgaaaagct
    31021 aaggcactaa taaaagaacg aaaaagtttg gttattagga gaactgataa tctgacaggt
    31081 ggaatgatga agtaacgctc tgaaacgcct gccctgagcc tccgacaagc attgtatgag
    31141 gaaactcaga atgtttgaag ctcggtaaag acggctgaaa gataccgtaa tagttgatga
    31201 acatatatct tctatcgaaa actatccaga ggtgggtagc gtatccaaca ggtcgggggt
    31261 ctataaaata tctatggtta gaatgttcta taaggaactg acgaaacagc gaatgtacgg
    31321 gtctaaattt gaactttgta gaaatgcaaa ggtacgagaa atcgtagctg gtgtggcaaa
    31381 gtaagaatat aggttatgaa atgccatata acgttacagg cggtatcaag ccagcaggct
    31441 tatagctagt gacctaagga tatatatgta tagataagat tatcggaacg aggaaaggta
    31501 ttgggttctc atcaagagaa taaactgacg aagaacaata agcagttgaa ctaatgctga
    31561 aaagcagagg gcgaacctaa gaaattcttg taatgagaat ggaggaatgc cctcaagtcg
    31621 tgtatatatt agaaaagtat tatgttgttt caataaggat tacgagtaag ataaaaaagc
    31681 tcactcaatt tcaagtaaag gagtgaagcc ttatgcctaa aaataaaaat gaaacattgt
    31741 gtgtagaaga tttaagacac gctgaatatt acgaaatgca aaacaccttt gatgatttat
    31801 atgccaaaag taagaatgga gatattttca ctcatcttat ggatattatc ctatcgagag
    31861 aaaatatatt actggcatat cgaaatatca aagcaaatgc aggcagtaaa acagcaggaa
    31921 cagacggtac aataattaaa gatattggca aattgccagc agaaacagtt gtcaaaaaag
    31981 taagatacat tgttgcaggc agtccacatg ggtatcgacc taagccagtg aggcgaaaag
    32041 aaataccaaa accaaatggt aaaaccagac ctctaggtat tccttgtatg tgggacaggc
    32101 ttatacaaca atgtataaaa caagtattag aaccaatctg cgaagctaaa tttagtgaaa
    32161 acagctatgg ttttagacct aataggtcag ttgaaaatgc aataaaagca acctataacc
    32221 gcctacaaat cagccagcta cattatgtga ttgaatttga tattaagggt ttctttgaca
    32281 acgtgaacca ttccaagctg ataaaacaga tatgggctat gggaatacgg gacaagcact
    32341 tgatattcat tttaaaaagg atattaaaag ctccaatcaa aatgactaac ggtacaataa
    32401 catatccaga gaaaggaact ccgcaagggg gaataatatc cccactacta gcaaacattg
    32461 ttcttaatga gttagaccat tgggtagaaa gccaatggca ggaaaatcct gttacaaaaa
    32521 actatgtggt tcatatcaat aaaagcggta gcccatgtaa aagcaacgct tacaaagaaa
    32581 tgaagaaaac aaagttaaag gaaatgtata tggttcgtta tgcagatgat tttagagttt
    32641 tctgccgcta caaggaaagt gctgaaaaag ccaaaatagc aatcactcaa tggattgaac
    32701 aacgattaaa attggaagta tcacaagaaa aaacaagaat tgtaaatgtt agaaaaagat
    32761 actccgactt tcttggtttc aagattaaaa tgataccgag aagaaagaag ttagttgtta
    32821 aatcacatat aagtgataaa caattcaaaa accaaaaaga caagctggta tcacaggcaa
    32881 agaaaattgc ttctccctca aaagggaaaa cggaattaga agaaacaaga ctttataatt
    32941 caatggtatt aggtatgcaa aattattatc agattgcaac gaatgtaaat ctggattgta
    33001 tgaaactgaa tagagctgta atggctatct tcactaacag attaggtaca aacaaaaggg
    33061 gcagattgag aaaaacaggc agacaactta cgacaaagga ggcaacaaga tatggtaaat
    33121 ctaaaatgct tcggtatgtt gctggcgagg gcgaacctat tttccctgtt ggatatatcc
    33181 aacacagaaa accaatggca aaggtgtatt tagcgaattg ctacactcta gagggaagaa
    33241 aatttatcca caccaatcta agtgttgata aattactgat gtaccagtta atgaaattat
    33301 ctttggaaaa tcgcacaata gaatacgcag acaatcgaat ttcgctattt tctgctcaac
    33361 atggaaaatg tgctataact ctggaagaat ttcaacaagc taatgaaata cattgtcatc
    33421 atattgtacc aactggagct ggtggaaatg acgattataa aaatcttatt ttggtaaaag
    33481 aggcggtaca ccgtctaata cacgctaaaa cagaagaaac aatacaaaaa tatatagctc
    33541 tattgaagct aaataaagtc cagctcataa gactaaataa atatagagta ttagctggca
    33601 atcaagaatt aaacttgata taggtctact acataaatac ttttcaatat aaacacgatg
    33661 gaacgcggag tgcggtgaaa gtcgcacgct ccgtgtgaat ggggggaaaa gtttgagata
    33721 tttataatat caggaactta cctatccata tatcaatggt ttttaaccaa tgcccaatcc
    33781 ttagtcttac tggcaattgt ggtcattggt ttgtttttag gatttaagcg ggagttttcc
    33841 aaactgattg gctttttggt gattgctttg attgcggtcg gattggtctt taatgcttct
    33901 ggggtgaagg atgtcttact cagtttgttt aaccgaattg tcggtgcgta agggggaagc
    33961 acgatggaac agatgcgtgt ttatattgcg aacctcggga aatataacga aggtgaattg
    34021 gtgggggcgt ggtttacccc acctgtcgat tttgatgaag tgaaagaacg aattgggtta
    34081 aatgacgaat acgaggaata tgcaattcat gattatgaac ttccctttga gattgatgag
    34141 tatacgccaa ttgaggaaat taatcggttg tgtggcttag cggaggaact tgaaggaacg
    34201 ccagttggtg aagttgcttc tgagattcaa catgctttct tcaattcttt tgaggagatg
    34261 gtagagcatg tggatgatat tatctattat ccagattgcg atgatatgag tgatgtagca
    34321 tatttcttga ttgtagaagc tggtgatttt ggagaagtac cagaacactt gaagacgtat
    34381 attgattttg atgcgtgtgg tagagacttg gagattggag gcaattattt ggtaacgagt
    34441 cgaggtgttt ttgagtatcc atattaacaa aattattgaa gggtagttga tttctaaact
    34501 atccttcttt ttaaaggaat gatttgatga agaaaataaa aagctacacc agtatttggg
    34561 cagttgaaaa ggtcatttat gcaattaatg actttcaact gccttttcct gtgactttta
    34621 accagatggc ttggtttgtt ttatccttgc tgtttgtcat cgtatttgcc catgtgcctc
    34681 cactatctat gatagagggg gcctttttaa aatacttagg tataccggtg gcagttactt
    34741 ggttcatgtc gcaaaaaacg tttgacggta agaaaccatt gggattttta cgatctttta
    34801 ttagctatca cttacgattt aaggtcactt tcgcaggaaa gaaagtcaaa gaacagaaga
    34861 aacgatggga cgaacccatt actctggtaa ggagtgtgaa ctatgtaccc gattaagtat
    34921 attgaaaata atcttgtctt taatcaagag ggagaatgtt ttgcctacta tgagttaata
    34981 ccttacaatt actccttttt gtcacctgaa cagaaatatc aagtccacga taactttcgt
    35041 caattgattg cgcaaaatcg tgaaggcaag attcatgcct tacaaattgc cacagaaagt
    35101 agtattcgtg ccacgcaaga acgatcaaaa aaggaaatta ccgggcgatt aacagaagta
    35161 gcgaaacaac gaattgattt acagacagag gcattagtat ccatgattgg cgatagtcag
    35221 attgattatc gcttttttat tggcttcaaa ctgattgcga cagatgaaga ggtcaatcta
    35281 aagaatctca agaaatcttt cttctccggc tttcaagaat ttgtctatgg agtgaatcat
    35341 catttgatgg gcgattttgt ttccttatcc aatgaagaaa ttcgccgata taccaagctt
    35401 gaaaaactaa tggaaagtaa attggctcga agatttaaag tcagacgagt cacaccgagt
    35461 gatctgatgt atttgattga acatatctat ggcgaaaagg gtacaccatt tgaagaatat
    35521 gaatttcagc taccaaagaa aaagctgaag tcggaaactt tggtgaaacg ttacgactta
    35581 ttacgtccca gtcgttgttt gattgaagaa aagccccggt acttatgcat ggaacatgag
    35641 aatcatgaat catatgtagc gtacttaaca attaatacaa ttgtggggga aatggagttt
    35701 ccctcctcag aacttttcta ttaccagcaa caacaattta cattcccaat tgacactagt
    35761 atgaatgtgg agattgtgac gaacaaaaag gcacttgcta ccgttcgcaa taagaaaaag
    35821 gaattaaaag acttggacaa ccatgcctat caatctgaca atgaaacaaa ttccaatgtc
    35881 ttagatgcat tggattccgt agatgaattg gaaactacct tggatcagtc caaagaatcc
    35941 atgtacaaac tgagctatgt ggttcgagta agtgccgaat cagtagatga actaaagcgc
    36001 cggtgtgatg aagtgctaga tttttatgat gataccaatg taaaactagt ccggcctttt
    36061 ggcgatatga tggggctaca tgaagaattc ttaccttcta gtaagcgata catgaatgat
    36121 tatatccagt atgttacgtc tgattttctc gccggacttg gttttggcgc cactcagatg
    36181 ttaggggagt tggaaggcat ttactttggt tataacgtag atacggggcg caatgtttac
    36241 ctgaaaccgg cattggcctc acaaggggtc aaggattcag ttaccaatgc gctagcagcg
    36301 gcattcctcg gttcactcgg tggcggaaag tcctttagta acaatctctt agtttattac
    36361 gccgtccttt ttggtgg
//
LOCUS       DASNNU010000020        35033 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.020, whole genome shotgun sequence.
ACCESSION   DASNNU010000020 DASNNU010000000
VERSION     DASNNU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.020"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..196)
                     /locus_tag="U8116_000931"
     rRNA            complement(<1..196)
                     /locus_tag="U8116_000931"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            937..1626
                     /locus_tag="U8116_000932"
     CDS             937..1626
                     /locus_tag="U8116_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN8433942.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1613..2821
                     /locus_tag="U8116_000933"
     CDS             1613..2821
                     /locus_tag="U8116_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN8433943.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            3115..4386
                     /gene="serS"
                     /locus_tag="U8116_000934"
     CDS             3115..4386
                     /gene="serS"
                     /locus_tag="U8116_000934"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEN8433944.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4935..6419)
                     /gene="guaB"
                     /locus_tag="U8116_000935"
     CDS             complement(4935..6419)
                     /gene="guaB"
                     /locus_tag="U8116_000935"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEN8433945.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6924..7619)
                     /locus_tag="U8116_000936"
     CDS             complement(6924..7619)
                     /locus_tag="U8116_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEN8433946.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7709..8809)
                     /gene="ychF"
                     /locus_tag="U8116_000937"
     CDS             complement(7709..8809)
                     /gene="ychF"
                     /locus_tag="U8116_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEN8433947.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8818..9009)
                     /locus_tag="U8116_000938"
     CDS             complement(8818..9009)
                     /locus_tag="U8116_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEN8433948.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(9030..9920)
                     /locus_tag="U8116_000939"
     CDS             complement(9030..9920)
                     /locus_tag="U8116_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEN8433949.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9907..10674)
                     /locus_tag="U8116_000940"
     CDS             complement(9907..10674)
                     /locus_tag="U8116_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN8433950.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10686..11399)
                     /gene="rsmG"
                     /locus_tag="U8116_000941"
     CDS             complement(10686..11399)
                     /gene="rsmG"
                     /locus_tag="U8116_000941"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEN8433951.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11631..13532)
                     /gene="mnmG"
                     /locus_tag="U8116_000942"
     CDS             complement(11631..13532)
                     /gene="mnmG"
                     /locus_tag="U8116_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEN8433952.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13549..14946)
                     /gene="mnmE"
                     /locus_tag="U8116_000943"
     CDS             complement(13549..14946)
                     /gene="mnmE"
                     /locus_tag="U8116_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEN8433953.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            15245..15628
                     /locus_tag="U8116_000944"
     CDS             15245..15628
                     /locus_tag="U8116_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433954.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15796..16554)
                     /locus_tag="U8116_000945"
     CDS             complement(15796..16554)
                     /locus_tag="U8116_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEN8433955.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16572..17390)
                     /locus_tag="U8116_000946"
     CDS             complement(16572..17390)
                     /locus_tag="U8116_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN8433956.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17387..17743)
                     /gene="rnpA"
                     /locus_tag="U8116_000947"
     CDS             complement(17387..17743)
                     /gene="rnpA"
                     /locus_tag="U8116_000947"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEN8433957.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17861..17995)
                     /gene="rpmH"
                     /locus_tag="U8116_000948"
     CDS             complement(17861..17995)
                     /gene="rpmH"
                     /locus_tag="U8116_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEN8433958.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18518..19852
                     /gene="dnaA"
                     /locus_tag="U8116_000949"
     CDS             18518..19852
                     /gene="dnaA"
                     /locus_tag="U8116_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEN8433959.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            20050..21180
                     /gene="dnaN"
                     /locus_tag="U8116_000950"
     CDS             20050..21180
                     /gene="dnaN"
                     /locus_tag="U8116_000950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEN8433960.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21409..21651
                     /gene="yaaA"
                     /locus_tag="U8116_000951"
     CDS             21409..21651
                     /gene="yaaA"
                     /locus_tag="U8116_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEN8433961.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21638..22762
                     /gene="recF"
                     /locus_tag="U8116_000952"
     CDS             21638..22762
                     /gene="recF"
                     /locus_tag="U8116_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEN8433962.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22759..24705
                     /gene="gyrB"
                     /locus_tag="U8116_000953"
     CDS             22759..24705
                     /gene="gyrB"
                     /locus_tag="U8116_000953"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEN8433963.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24725..27196
                     /gene="gyrA"
                     /locus_tag="U8116_000954"
     CDS             24725..27196
                     /gene="gyrA"
                     /locus_tag="U8116_000954"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEN8433964.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27432..27731
                     /locus_tag="U8116_000955"
     CDS             27432..27731
                     /locus_tag="U8116_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEN8433965.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27781..28293
                     /gene="ssb"
                     /locus_tag="U8116_000956"
     CDS             27781..28293
                     /gene="ssb"
                     /locus_tag="U8116_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN8433966.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            28319..28555
                     /locus_tag="U8116_000957"
     CDS             28319..28555
                     /locus_tag="U8116_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEN8433967.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28698..30671
                     /locus_tag="U8116_000958"
     CDS             28698..30671
                     /locus_tag="U8116_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEN8433968.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGYFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30677..31129
                     /gene="rplI"
                     /locus_tag="U8116_000959"
     CDS             30677..31129
                     /gene="rplI"
                     /locus_tag="U8116_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEN8433969.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31403..32770
                     /gene="dnaB"
                     /locus_tag="U8116_000960"
     CDS             31403..32770
                     /gene="dnaB"
                     /locus_tag="U8116_000960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEN8433970.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33550..34842
                     /locus_tag="U8116_000961"
     CDS             33550..34842
                     /locus_tag="U8116_000961"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEN8433971.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10648 a         6822 c         6929 g        10634 t
ORIGIN      
        1 gattgttata cggtattagc acctgtttcc aagtgttatc cccttctgat gggcaggtta
       61 cccacgtgtt actcacccgt tcgccactcc tctttttccg gtggagcaag ctccggtgga
      121 aaaagaagcg ttcgacttgc atgtattagg cacgccgcca gcgttcgtcc tgagccagga
      181 tcaaactctc ataaaaagtt cgaacaatct tgcgattgtt aagctcaatt tgtttgctag
      241 catattgctt gcttgttaaa aatgtttgtt gtcttgaatt gaatcaagac gccctacaca
      301 tttggtttgt cttactttgt tcagttttca aaggtctact tcatcaggag tgtttcccga
      361 ttcgtttttg gctgccgtag caacttttat atcatatcag agagacaagt aattgtcaac
      421 tcttttttaa aagttttttc gatttcgttt ttcgaaatct tccagcaact catttatcat
      481 aacttatcgt ttgttatttg tcaagaactt ttttgaagtt tttttcgata gaatcaagct
      541 tcgcaagtgg ttctttttta acgactcagt tatcttatca tgtcatttcc aacatgtcaa
      601 caacttattt tttattattt aataagttat tggtgttctt ttgaacaaat aataatatat
      661 caacatcaca tgtgaaagtc aataagaaaa tgcttattta tcaacatttc tagcttacat
      721 gatcattggt tagttctggt cctttttcgt tcgtctttat aactccgaga gacgtaaaat
      781 ccttttgttt tcagtttaat gttcatgttc ttgtcttatt cacaccaact agatgtctca
      841 ccttctaaaa ttttccttaa caaagtgctg cttttatcta tttctgcttt tttttgctag
      901 aataatagta acaatatgac tctggaggat ttatttatga aaattttgat tgctgacgat
      961 gataaagaaa tcgttgaatt attaagtatc tatgttcaca atgaggggta tgaagcagta
     1021 aaggcttatg atgggaaaga agcattatct aaaatcagaa caatccctga tattgaatta
     1081 cttattttag atatcatgat gcctgagatg gatgggatgc aagtagtgaa agagctacga
     1141 aaagaatccc aaataccaat cattatgctg acagcaaaaa ctactgatat ggataagatc
     1201 aaaggactgg tcgctggtgc agatgattat gtgaccaagc catttaatcc gttagaagta
     1261 atggctagag taaagtcttt attacggcgt acaaaaatgc agattgcccc agatcagcct
     1321 gatgtattag aagtaaatgc attaatcatc aataaagact ctcatgaagt taaaacaatc
     1381 gatgggaaag aaatccagtt aacagctttg gaattcggta tcctctactt gcttgctagt
     1441 catcctaatc gagtatttag cgcggatgaa atttttgagc gtgtttggaa acaagaaagt
     1501 attgtctctg ctaaaactgt catggttcac gttagtcatt taagagataa gattgaagaa
     1561 gcaactggtg gtgaaaaagt cattcaaacc gtttggggag tgggctataa aattgacgca
     1621 agatagtaaa cgagcaactg aaaaacgccg tcggatcaat ttgacttcaa aagaaatcag
     1681 cgaattgtta gcggaaggaa tcatcacgat tatccttttg cttctattaa atgtttctat
     1741 tctggttgtt atcagttcgg tgatcaacag ttcaccttct ttgacgaatg cgatttggga
     1801 ttcaaagaat atttttgcag aacgattgaa cactgatttg ttctggaacg gccggaattt
     1861 tattattccc tttttctttc tattagatat tggtgttttg tattggcgct tgattcgaag
     1921 atatcgccaa atgcaattgc ggcatatcat cagcgaactt cactacatcg caaacggaaa
     1981 ttatgatcat cgtatccctt ttgaactgag tggtgactta agccgagtgg taacaagtat
     2041 caatggattg gtggatagta ccgtagcagc gattgaagac gaacgtaaaa tcgaaaaatc
     2101 aaaagatgaa ttgatcacga atgtcagtca tgatatccgt accccactca cgtccatcat
     2161 tggataccta ggactgatag aagatggcca gtatcactca gaagaagatc ttttaaagta
     2221 tacgcatact gcttatatca aagcaaagca gatgaaatct ttagtggacg acttatttga
     2281 atacactaag gtacgccagc ctgctgtccc ggtgaatttt tctgcctttg acatgatcca
     2341 gttgatcgaa cagttagcag ctgattttga gttggaagct tctaaaaaaa atatccaaat
     2401 acttgttcaa tcaaaagtag actcccttat catggatggc gataccgaaa aacttgtacg
     2461 tgtcttcaat aatcttttga cgaatgcgtt gaaatacgga aaaggagcaa cgaagatcgt
     2521 aatcgaagta gaacgtatcg gttcagaagt tgttgcaaca gtcaaaaata acggagcaat
     2581 gatcccacaa caggcaatcg ataatctatt cgaccgtttt tatcgtgtag aagaatctcg
     2641 ttcacaagca actggaggaa ctggacttgg tttagcaatc gctcaaagca ttgtggcttt
     2701 gcacggcgga tacatttatg caaaatcaga taagcaatgg acttctttca tcatgcacct
     2761 tccaatcaag aaaaatgaaa aattacctat tgatacacag gaagttattg acgaaagctg
     2821 aaatattcgc tattcttatt acaattatta gaataaatga aaaacacaga acccagtaca
     2881 ctttattctt tttcagagag ctgatggcag gtgtaaatca gcaaaggata atttggaagt
     2941 tccagttcgt ttacgtgccc tttcgaaata agaaaggtcg gttcaacagc cgttatgtgt
     3001 tcaacaagtg cagctgacgc tgaacttggg tggtaccgcg aatttcagac tgattcgtcc
     3061 caagaataat gggatggatc agtctttttt tattttcaaa aaggaggaaa tcatatgtta
     3121 gatgtaaaaa tgatgcgaca aaatattgaa gaagtacaag aaaaattgat gactcggggc
     3181 gtaaaaaaag aaacattagc tgaattcatg gaactagatg aaaatcgtcg taagctacta
     3241 gtcaaaagtg aagaattaaa aaaatatcga aatgatgttt ctgctgagat cgcccaattg
     3301 aagcgtgcaa aagaagatgc ttctgctaaa atcgctgaaa tgaaagaagt cggtggcaac
     3361 atcaaagcat tggatactga aattgctgaa gtggatgaaa aattgaaaca aattgcaact
     3421 actttgccaa accttcccca tgattctgtg ccagttggaa cagatgaaga ggacaatgta
     3481 gaaatccgtc gttggagcga accacgtaca tttgcttttg aaccaaaacc acactgggaa
     3541 attgcagaaa atcttgatat tttagatttt gaacgtggcg caaaagtagc cggtagccgt
     3601 tttgtctatt acaaaggatt aggtgctcga ttagaacgtg ctgtctacaa cttcatgctt
     3661 gatcagcata tttacgaaca cggctatact gaaatgatca ctccttatat cgtcaacagt
     3721 aaagcgatgt ttggtacggg acaatttcca aaattcaaag aagatgtttt ccaattggag
     3781 aatactgatc taacattaat cccaacagct gaagtaccat taactaacta ttataatgat
     3841 gaaattttag atggtgcaga tttacctatt tatttcactg cactaagccc tgcttttcgt
     3901 tctgaagccg gaagtgctgg tcgggataca cgaggactga ttcgtcttca ccaattcaat
     3961 aaagtagaaa tggtcaaatt ttcggatgta gagcattctt acgaagaatt ggaaaaaatg
     4021 acagctgatg cagaagatat cttgaaaaaa ttgcatttgc cttatcgtgt gatggcctta
     4081 tcaactggag acatgggctt ttctgcagct aaaacttatg acttagaagt atggatacct
     4141 gcacaagata cttatcgtga aatcagttct tgttctaact gtgaagattt ccaagcacgc
     4201 cgcgcaatga tccgttatcg cgataatgat ggaaaaatcc actatactca tactctgaat
     4261 ggttcaggac ttgcagttgg ccgtacagta gctgccattt tggaaaacta ccaaaatgaa
     4321 gatggttctg tcacagttcc agaagcgctc gttccttata tggggaatct gaaagttatt
     4381 aaataaagag gctgtgaaaa agccgcttag tttcgagtat taagaaaaat tttgtgaaaa
     4441 tggctttcca catttttgca taaattggac ttaataccga gaaactggct tttgaacacc
     4501 atttatcgag aggttgtgaa atcagcgaat taacctgagt attagagaaa aaaatggaaa
     4561 aatagtccct catattttac cgtttgttga actaataccg agggtcagct gattgaacac
     4621 cgtttagtgg agaggtcgta agcctacccc ccagctccta gttacaagga caaattcaaa
     4681 aaacagctct tcatatttta tgaatgttgt acttgtgatt cggaggtggc aagggaacac
     4741 tgtttattca aaaagaggct gggacagaag cgtttaactc caagaaataa gaagaaattc
     4801 acgaaaattg cttttcaaat ttttgtgaat ttcttcttat ttccgaagga gttgcttcta
     4861 cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt gttctttact tttgtcccac
     4921 gctctttaat gtttttaaga ttcaactgaa tagtttggtg cttcttttgt gatttgtaca
     4981 tcatgagggt gtgattcttt taatccactg ccgctcattt ggataaattg cgcttcatca
     5041 cgtaattgct gtagattggc tgccccaaca taacccattc ctgaacgcaa accgccgatc
     5101 atttggaaaa taatatctgc aacacttcct ttataagcaa cccgaccttc gattccttct
     5161 ggtactaatt tatttgcttc attgacacta ccttggaagt aacggtcttt tgaacctttt
     5221 tccatcgcgc ctaatgagcc catacctcgg tatgttttga atcgacgtcc ttggaagatt
     5281 tcgaattcac ctggtgattc atctgtccct gccaacatac ttcctagcat cactgcatgt
     5341 ccgccggcag ctaatgcttt gacgatgtca ccagaatatt tgatccctcc gtctgcaata
     5401 atcgctttgc catattgacg agcaactgat gctgcatcat aaatagctgt gagctgaggt
     5461 acacctacac cggctaccac acgtgtcgta caaatcgatc ctggtccgat accaactttt
     5521 acgacatcta cacctgcatc atataaagct tttgtcgctt cagccgtcgc aacgtttcct
     5581 gcaatcaatg ttgcatctgc aaaagttgaa cggatttctt gaatttttcg gatcacacct
     5641 gcactatgtc catgagctgt atcgatgatg attgcatctg cccctgcttc taataaagca
     5701 ttcgcacgct caaatgtatc gcttgttaca ccaacggctg ctgctacaag caatcgacca
     5761 tgttcatctt ttgctgcatt tggaaattcg atcactttct cgatgtcttt gatcgtgatc
     5821 aatccgctta aacgaccttc gttatctacg attggtaatt tttcgatttt atgtttttgc
     5881 aaaatctttt ctgcatcttt tagagaggta cctactgggg cagtcactaa gttgtctttt
     5941 gtcatcacat cagcaatcgg catgtgataa tctgttacaa aacgcatgtc gcggtttgta
     6001 attatcccta ccaattttct gttttcaagt gtttcaacga ttggcacacc actgatacga
     6061 tatttgctca tcaattcttc tgcgtctgct acaagatgct gtggcgttaa gaagaacgga
     6121 tcaatgatta cgccactttc agaacgtttg acttttctta cttcatccgc ttgctggctg
     6181 atcgtcatgt ttttatggat cacacctagt ccgccttgtc ttgccatagc aatggccatc
     6241 ttgctgtcgg taacagtatc catacttgca ctgatgattg gaatattcaa cttaatattt
     6301 ttagctaatt gtacgctcat atccacttcg tttggtaaca cgtgactttc tgctggtatc
     6361 aataagacat catcaaaagt aaatcctttt ttcgtgaact ttgtttccca gttggacatt
     6421 tttagtacca ctcctctaat ttagtttatc tattaaaata attgattttt tcatttaagt
     6481 caatgatcta cttggaatca agttagcttt ctcatagaat accaatataa agcgtttaca
     6541 tctgtgtgta ttaaaagaga ttgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc
     6601 aatcaaaaac agcttctcat gtttttgaat tggttgggtt tattaccgca gatgcaaact
     6661 tttgaacacc gtttagtgga gaggctgtga aaaagctgct tagtttcgag tagtaagaaa
     6721 aattttgtga aaatggcttt ccacattttt gcataaattg gacttaatac cgagaaactg
     6781 gcttttgaac accgtttatc gtaaaaaggg actgtgacaa gacttttgtc acagtccctt
     6841 actattattt aactatggat aaacaaaatg aggttcttta ctacccctag atatctagca
     6901 aaatagctga tctttttgct agtttatctt cttgggacgg ataaacttcc aataatcccg
     6961 tattttttct tcaagtagta acggattgct aaagcagttg ccccgataat gataactact
     7021 actggatcta atactggatt gataactggt ggcaatagtg ttgtcgcaga aaacagtacg
     7081 acccaaagaa gtgtcgttcc acctaggata agcaatgtct tacccattcc tggtttcttg
     7141 cttcgatctg ctccgggata ttcatattga tagatgtatt tgtacatcat ataaaagacc
     7201 cagccaccga ttgcagaacc tagaacgagt gtagaaaagc cataagctgg cgttgttcca
     7261 cgtgaaaaca atccggtaat cgcaatcatg atacttaata gaccaagcaa cagcaacgta
     7321 ttatctaacc acatcaataa aggggtggat gaatttttta cttctgctgg cttattgatg
     7381 attgcttccg ttctttcaga gactgtccca aacaattgac gggctgttct tccgcctttt
     7441 tgttctgcaa ctaaagtagg cagaatgtca tgaagggctt ttactttttc ttcttcagac
     7501 aaattcgctg cttctaaaga ttttttcaaa tcaaaaatgt actgttgatt tcttttggtt
     7561 aattttgttt ctaaactatt gttttctttt acgattgctc gtaattgttc tggttccatt
     7621 atctaatagt cctccttagc cgttcctttt ctcaacggtc gtcacaaaag acattctacc
     7681 atattctaca gctagtttga tagccctttt acacattaaa gcggaataac attacgtcgc
     7741 cgtcttgtac gacataatct tttccttcta atcgaacacg tccagcttct tttgctgcat
     7801 gcatgtttcc gtaatgatcc aagtcttcaa aagagacagt ttctgcacgg ataaatccgc
     7861 gttcgaaatc tgaatggatg attcctgctg cttgtggtgc tttgattcct tttttgaatg
     7921 tccaagcacg tacttcttgt tccccagcag taaagtaagt agctaagcct aacaaatcat
     7981 aagcagcacg gatcaactga tccaaacctg attcttcgat tcctaaagct tctaaaaatt
     8041 ccgctttgtc ttcttcatct aattctgcga tttcttcttc tgcacgagca ctgaccacga
     8101 tcacttctgc attttcttct gcagcaaagt tgcgcacttc ttgtacataa tgattcgcgt
     8161 ctgcatctgc tacgtcatct tctgagacat tggctacata aagaattggt tttgtcgtca
     8221 ataaaaatag tgatttaacg attttttgtt cttcttcagt aaattcgatc gttcgggcag
     8281 attttccttc ttccagtaca ggtttgattt tatccagtac agctagttct gccactgcat
     8341 ctttatcttt ggttttagca acttttgcta cacgagtata gcgtttgttg atagattcta
     8401 agtctgctaa cactagttcc aagttgatcg tatcaatatc ttctagtgga tcaacacgtc
     8461 cttctacgtg agtaatatta tcatcatcaa aacagcgaac aacgtgacaa atagcgtcta
     8521 cttggcggat atggcttaag aattgatttc ccaaaccttc tcctttactt gcgcctttta
     8581 cgattccagc aatatctgta aattcaaaag tcgtcggaac agtttttttc ggatgtacaa
     8641 gttctgttaa gcgttgcaaa cgccagtcag gtacttctac catccctacg ttggggtcga
     8701 tcgtcgcaaa gggatagttt gctgcttctg ctcctgcttt tgtgatcgca ttaaacaaag
     8761 ttgattttcc tacgttgggc aacccaacaa ttccagcagt caatgccata aatttattca
     8821 ctctttctct gcattttttt caattacttt ttttaatttc ttttcaaaat ctcgacgggt
     8881 catcatgacg atatgtccgc aattcataca tctgattttg atatctgcgc ccattcgaat
     8941 gacttcccag cgattcgtct ggcaagcgtg aggttttttc atttcaacga tatcgtgcaa
     9001 gtcgtacata aaaatagcct cctttttgtt tattcttcat caaattgaat attcaagata
     9061 tcaagaattc gagtcaaatc tttttgagaa agatattcaa tctcgatctt acctttccca
     9121 tttttttctt ggatagctac acttgtacca aatttatcca tcaaacgatc ttcgctttct
     9181 cggatataat atggtttttc ctttgctagc cttgggattt ttttcttttc ttttccatca
     9241 ttttgattta actcagaaac aagctgttcc agttggcgga cagttaaatt ttcagttact
     9301 gcacgattgg ctaatttcaa gatcaatgtt ttatttttta aacctaacag cgtacgggct
     9361 tgtcccatag ataaacgctg atcttgtacc atttccttga caagatccgg taaagaaagc
     9421 aagcgcaaat aattggcgat ataaggtctg ctctttccta aacgactagc tacttctgct
     9481 tgtgttagtt tcaggttttt catcagcatc tcatatgctt ctgcttcttc taacggattc
     9541 agatcttctc gctgcaagtt ttccagcact gctacttgca tcatcgcttc ttcgtcaaag
     9601 tctctaacga tagcagggat cgtttctttt tcagctagtt tggacgctcg aaaacgtcgc
     9661 tcccctgcaa taatctcata tccttttact gcggaacggc gtacgatgat gggttgaaag
     9721 acacctgatt gttcaataga attagctagt tcctgcaaag atacttcttc aaatgtttta
     9781 cggggctgat acggatttgg tcgcagctca ttcaatggta gctggacaac ggtatcatct
     9841 tttacatcca ccgtctctaa gttttctaaa tcttgaaaca gtgcatcgat tcctcggcct
     9901 aatcctttac ttttcttcac gacttaccac ttcctttgcc aatgcttggt agacttcagc
     9961 tcctcgagaa cgtggatcgt agtcgatgat cggttttcca tgactaggtg cttctgataa
    10021 acgaatattt cttggaatga tcgtttcgta aactttttcg cggaagtatt tacgtacttc
    10081 ttctacgact tcatttccca gatttgttcg tgcatcatac attgtcagta agactccctc
    10141 gatctctaat tctggattaa agtgtttttg gactaaacga accgtattca gtaattggct
    10201 taatccttcc aatgcgtaat actcacactg aacaggtatc aaaatcgaat cacttgctgt
    10261 aaatgaattg attgtcagat ggccaagaga aggcgggcaa tcgatcaaaa tataatcata
    10321 ctgactgctt acttctgcta aagatccttt taatcttgat tctcttgcca tcattgacgt
    10381 caattcgatc tctgctcctg ctaattggag agtcgcaggc acaatactca aattttcgtg
    10441 ttcagtgatc aaagttgcct catcaattgg caattcattg actagaacat catagatgtc
    10501 tcttgtcaca tcaggttttc tgatcccgac accactggta gcatttcctt gtgcatccat
    10561 atccactagc aatacttttt tgccaaggct agcaagacaa gcacctagat tcaccgtcgt
    10621 cgttgttttg ccgacgccac ctttttggtt cgcaacagat attatttgtg ccatctcttc
    10681 tcctcctatt tgattggctg cttattaggc agtccgggct ttcttgggta tttctttggg
    10741 gttggtttct ttttttgaat aacgatgata tgccgttgat cagagacatt cggcaaggta
    10801 accgcctctt ctttgaggaa tttaccacct aataaagcaa tcgcttgttt tgcttctgtc
    10861 aattcctctt cactttttgc tgctttcaat gcataaaaat agccttcttg tttagccaag
    10921 ggcaaacaca attccgctaa cacattcaaa cgagcaaccg cacgagcagt gacaaggtca
    10981 aatgatgcac gaaagtgagg attttgccca aatgtttctg cccggtcatg atataaggaa
    11041 gcatcgattc ctaataatcg ggttaactca gataagaagg ttattcgttt attcagagaa
    11101 tcaacgatcg ttatctgcag ttctggaaag gcaattttta aaggtatgct aggaaaccct
    11161 gcccccgaac caacatcgca taaagataaa ggttgcgtaa agtctacttc aaatgctaat
    11221 gtaagtgaat cgtaaaaatg cttcagatat acgtcttttt catccgtaat agctgtaagg
    11281 ttcatctttt cgttccattc gactaatgtc cgataataaa tagcgaattg attcatttga
    11341 tgatcggtaa gctctatccc tttttctgcc aaggattggc gaaattcttc tggtatcata
    11401 aatattcctt tcattgtgaa acagcaattg tttcacagat tctctcccta ctttaacgca
    11461 aaatctctag acatgcaatc cttccatatg gattcttaaa aatttgaaaa gtaagaaaag
    11521 ccgatagact ttttctttct agtatgactc ataaaatata cacagaagag agaactagct
    11581 ttgagcaatt tctgcaaaaa ctagttctct cttctattta ttattacgta ttacgctggt
    11641 tcactgactt tcgcaatctt cccttgttcg atatagacca ttaggatact taggtctgca
    11701 gggtttacgc cactgattcg gctagcttgg gcaattgttt ctggttggat tttctgcaat
    11761 ttttgttttg cttctgttgc taatccgttg attgctgcgt agtcgatgtt ttctggaatc
    11821 tttttcgctt ccatgcgttt taatttttct actttttcca tggctttttt gatgtagcct
    11881 tcatatttga tttggatttc tacttgttcg atttcttttc gtgtcaattc ttcattttct
    11941 ggaataaacc gtagaagttc tgcataagta atctcaggac gtcttaaaaa gtcgcttgct
    12001 agtacaccat cttttaatgg tgccgcatta atcgttgcta aaaagtcttg gacttcttct
    12061 gttggtttga ttcgtacatt catcaaacgt ttgatttctt gttcaactgc ttcttttttc
    12121 ttcaaataat tcttgtattg atcttctttt actaagccta cacggtgacc catttctgtt
    12181 aatcgcaaat ctgcattatc atgacgtaaa agcagacggt attcagcacg tgaagtcagc
    12241 aaacgataag gttcgttcgt tccttttgtt accaaatcat cgatcatgac accgatgtaa
    12301 ccgtcacttc gtttgatcac taaaggttct tttccctgaa ctttcaaagc tgcattgata
    12361 ccggcaatca gcccttgtcc agccgcttct tcataaccgc ttgtaccatt tgtttgtcca
    12421 gctgtataaa gattttcaac gactttagtt tctaatgtcg gacggagctg gtgtggctct
    12481 actacgtcat attcgatagc atatcctgta cgcatcattt ctactttttc taatccagca
    12541 atagaatgaa gcatgttgac ttgaacatcc tctggtaaag aagtagataa accttgaaca
    12601 tagacttctt ccgtatttaa gccttctggt tccaaaaata attgatgacg tggtttgtct
    12661 gcaaaacgca cgatcttatc ttcgatggat ggacaatagc gagcgcctac cccttcaacg
    12721 atccctgtaa acattggggc acgatgtaag ttgtctcgaa taatttggtg agtcgtctca
    12781 cctgtatagg tcagccagca agattcctga tctagtttat aggcactatc tggtgtgctg
    12841 aagctaaaat gattcggctc tttgtcacca ggctgctctt ctgtaacaga atagtcgatc
    12901 gtacttgatt ttactcgagg cggtgttcct gttttgaacc gttcgatagc aaagccaagt
    12961 tcttttagat gattcgctag tccaatagaa ggttgagagt tattcggacc tgatgaatat
    13021 ttcaattcac cgataatgat ttctccgcga agtgctgttc cggctgtaat aacaactgct
    13081 ttggcccggt agctcgctcc tgtggacgtc actactccat gacaaacacc atcttcgacg
    13141 atcagttctt cgacgattcc ttgtcttagc gtcaaatttg gttctttttc gatcgtatgt
    13201 ttcatttctg ttgaataagc atgtttgtct gcttgtgctc gcaatgctcg gacagctggg
    13261 ccttttcctg tgttcaacat acgcatttgg atataggttt tatcgatatt cttacccatt
    13321 tctccgccta acgcatcgat ttctcgaacg acaacgcctt tagctggtcc gcctacagaa
    13381 ggattacatg gcatgaatgc taccatgtcc aaatttattg tcagtaataa tgtttttgat
    13441 cccatacgtg cagcagcaag tgcagcttct gaaccggcgt gtcctgcgcc aacaacgatc
    13501 acgtcatacg catctgcttc ataatgttcc aatcgtttgt tcctcctttt attttcctaa
    13561 acaaaattga ctgaataatt gagtgattag ctcatcttgt acactgtcgc cgacaacttc
    13621 tcctagatag tcccagcaac gcgtcatatc tatttgaacg agatccacag gcattcctga
    13681 atcaatacct gcaatgactt cttcaagtga gagggaagct ttttctaaca acgcgatatg
    13741 gcgggtatta gaaacataag tagcgtctct ttctcctgtt tctccgctga agaataaatc
    13801 agaaatagct gattccaaac gatctagtcc gtcgttttta gctacggaaa ttgaaaaaac
    13861 tggttcattt tcgatcaatt tcttcaattt ttcttgttcc agctgtgctg gcaaatctgt
    13921 tttatttaga agaatgatcc gtttcaatcc acttgtcgct tccaataatt gttcatcttc
    13981 tgctgtcagt ggttcgcttt ggtttagaac aagtaagatc aagtctgctt ctgctaatgc
    14041 ttttcggctt ctttcgaccc cgatccgttc caccacatct tctgtttccc gaattccagc
    14101 tgtatcgatt agcttgagcg gtaccccccg aacattgaca tactcttcga ttacatctcg
    14161 agtcgttcct gcaatatccg taacgattgc tttttcttct cttaacaaat ggtttaacaa
    14221 acttgatttg ccgacatttg gccggccgat gattgccgta cttaaccctt cccgcagaac
    14281 cttcccttgc ttggatgtag caagtagcgc ttggattctt tgttggatca tctgcgcttt
    14341 ttcaagcaat aacttcgtag tcaattcttc gacatcatca tattctggat aatcaatatt
    14401 tacttctact tgtgctaatg tctctaaaat ctcttgtctc aatgagcgga tcaaagcaga
    14461 cagattgccg tctaactgat taagagctag tcccattgct ttgtctgttt ttgcccgaat
    14521 caaatccatc acagcttctg cctgtgaaag gtcaacacga ccattcaaga atgctcgttt
    14581 tgtgaattcg cctggctcag ctaatcttgc accttctctt aacaatagct gcaaaatttg
    14641 attgactaca acgatcccac cgtgacaatt gatttctact acatcttccc gcgtaaatgt
    14701 ctttggtgct ctcatcacgg agaccatgac ttcatcgaca agctgattgc tcttgggatc
    14761 aacgatatgt ccataatgga tcgtatgact tggtacttct gataatcgtt tgtttccaga
    14821 ttgataaact ttatctgcta gttgcacagc ttgatcgcca cttaaacgaa cgatactgat
    14881 tgcaccttct ccaggtgggg tagaaattgc cgcaatcgta tcaaattcta aagtaatttc
    14941 agccattttt tcacttcctt tttcattttg cgcacaaaaa aagtgcccac tccacccttt
    15001 atgcacaata aacggtgaac ttcgcacttt gactgcttgt tcttgattaa attctggtca
    15061 tagattatca cacttgctat aaggaatcaa gtgtttaaaa caattagatg aagttgagcc
    15121 tgttctgctt ccattacctt atatttaatt gtccttggat gtctctttgc gttctttggt
    15181 ttattcgaag agaagatact gtataaacaa ggctttatct ttcttaaaaa aaacggtaca
    15241 ctaaatggta gcgctatcta aactatacac ttttgaaagg agaaaatatg tgagcccgtt
    15301 cagatctgct gcattggaag aagaaagtga ccctcggatc aacgatgtcg ttttatctac
    15361 agggaatatc aaccagaaat atatcgtccg cgatatcgtt tttgttgtgg aatgcttaga
    15421 agctgatttg tttgacccat cttttcccaa agatgacctt ttcttaacaa cgaagaggaa
    15481 attaaagacg aaggtacttg aatatggtgc gaatgcagta atcaactgtc attttgagta
    15541 tcagcaaaaa aaacgcgacg gaaagtattt tctggaactt tttgcttatg gaacagtgat
    15601 ccaatttatc caaacaacga ttggttaaat gaaacagaca aaaaaagcgg atcaaaactg
    15661 aagacagttt cgatccgctt ttttctatca aagttagtga ctacttcatc gtatactctg
    15721 ataagaatga tttctagtaa aatatacttg gtttgaaaaa ctttgtcaac gtgatttatt
    15781 caaaaagtaa tggaatcaat aatatttttt agcaggttct actactaaat aacgataagg
    15841 ttcctcacct tccgaatgtg tttttacatg ttcatctttg cttaatgcag catggatttg
    15901 tttacgttca aatgcaggca ttggttctaa gaaaacaggc tgccctgtcc gttttacttt
    15961 ttcagccgtg cgatcagcta aacgctgaat gatctcttgg cgcttttcac ggtaattgcc
    16021 tacattgact actacagaca gcttatttct tgctaaacga tggatgaaga cttgtgccag
    16081 atattgcaat gcattcagtg tttttccatg tttcccaatc agtatgcctt gcttttgtgt
    16141 ttctaaatgg aaaacaacca tattatttga ttctctttcc attttgacaa gagctggagc
    16201 atcaagctgt tttgaaattt cagtaagata aactgccaat tccgttaaag ctgcttcatc
    16261 acctagctca cttacttctt ctgttttttc ttcaagaatc gtttcgttga ctacctcgat
    16321 ctcagctgtt ggttcttctt ccaaaatttc ttctacagtc tgttctactg tttcagaaac
    16381 tttttcgctg atacttggct ggatcgaaag ccgtgcattc tttcgaccca ttcctaggaa
    16441 accttttttt ccttcttcca gtatttcgat ttcgacttgg tctttggcca aacccaaatc
    16501 agccagacct agttggacag cttcgtctat tgttgcagct tcataaatcg gcattagcat
    16561 tacctcccct attatttccg ttttttctta ggattcattg ctttcttaag agcacgttct
    16621 ctttcgcgga ttctccgtgc ttcttcttca cgctcttttc gaattttgaa agggttgttg
    16681 atcagcagcg tttgtacaac ttggaaggca ttggaaacta cccagtataa tgaaagaccg
    16741 ctggctaaat tgacacccat cactaaaatc attacaggca tcacgaaatt catgatcttc
    16801 attgatgcat tcgattcaat ttgactcatg ctagataaat acgtacttgc aaaagtaaag
    16861 attgctgcta aaatcggcaa aataaatgtt ggatccgcgt tccctaagtt caaccataag
    16921 aaatgccctt gtttcaactc tggtacacgt gaaatagatt gccataacgc catcaagatt
    16981 ggcatttgta ctaataaagg aagacaacca gcatatggat tgacgccatt ttctgcatat
    17041 aaacgctgct gctcttctct taacttactt tgtgtttccg gatctcttga actatattgt
    17101 tgctgcagtg cttttaattt cggttgtaat tcttgcgttt tacgcatgct ctttgtttgg
    17161 aaatgcatca acggcaatag gatcacgcgg atgatcagtg taaataaaat gatccctatc
    17221 cctgcattac caaatgacaa cgccttgatc gcctcagcaa agtaataaac aatataacga
    17281 tcccaaattc ctgtactctg tgcgctgact tccccagttc cgcaaccgga taagacgatg
    17341 accaaactaa cgataccggc catcaaaagt aaacgtttgt atttcttcac ttactctttt
    17401 cctctctgtc gattattttt gcaagtttta agacgtggac taaattggag cgaacctcat
    17461 cataagtcaa ccccttcaca cttggtctag caatcacaat aaaatccaac tccggatcaa
    17521 tttcttcttt tatagaataa actgctgctc ggatttttcg tttgacttca ttgcggttta
    17581 ccgcgttgcc gacctttttt cctacagata aaccaactcg aaaatgaggc tgtcccggtt
    17641 tttccaaacg gtaaacgacg aatttgcggt tagcaaaaga atctcccgta tggaacacgg
    17701 cttgaaactc tcgttctttt tttactcgat acgcttttct cattagtttt ctttcctttt
    17761 ctatctctca cttacgctca taatcatacc ataattacga ttgtcctttc atcatttccc
    17821 taaaaaagag tataaaaaaa accactgaaa ccccagtggc ctaagctgaa agaacttttc
    17881 ttcctttgcg acgacggcta gctaacacac gacgtccatt tttagtactc atacgtttac
    17941 ggaatccgtg aactttttga cgtttacgtt tatttggttg gtatgttctt ttcatatatt
    18001 ccacctccaa aatgttctta tctattttat aaacacagac atactttgat agtataacga
    18061 caaagtagtg attttgtcaa tataaaatag aagatcgatt gttaaaactt atccacaaga
    18121 ttattttttt ctatgggaat cctatgtgga aaaagttatg cacagggatt atccacagat
    18181 aataaaaggc tggttttaca cagaaaacac acgtgtaaaa aaagtaatcc acaatcaggc
    18241 gatttcctgt gtattccgtc tcctaactat tggtatatct gtatttttta tccacagacg
    18301 caagaggaaa tctattggtt tttacgagtt ttccacctgt gcattagtgg tgtggaaaac
    18361 tttttttgac aactgtgttt ttctcttttt tctttttgtc gattgtgtgg ataattgtaa
    18421 ttaaaaaatt tttttcttcg gtttctgtgg aaaactattg aaaaaaatgc taaattagta
    18481 ctagttattc actttttaat gaaggaggag gccttatatg gtatccctcg atgctttatg
    18541 gaatgaatta aaagcaacat accaaaagga tttatcgcct gctagctata atacatggat
    18601 cgaaacagcg aaccctcgaa cccttgatca aaaccagctc gttgttgagg ttcctagcaa
    18661 gattcataaa gaatattggg aaaaaaacct ggcgacaaaa atcgttgaag tcggttatat
    18721 gttatcagga aatgagatta tccctcgctt tattactggc gaagaagcag agcaagaaga
    18781 agttatagaa gaaaaaaatc caaaagttgt ggcacctagt ccgctaaaaa aagccatgct
    18841 gaaccctaaa tatacctttg atacgtttgt cattggtaaa ggaaaccaga tggctcatgc
    18901 agcagcgctc gttgtagcag aagatccagg ttctatttat aacccgctct tcttttatgg
    18961 aggcgtggga cttggtaaga ctcacttgat gcatgcaatt ggtcatcaaa tgctgcaaag
    19021 tcagcctaat gccaaagtaa aatatgtaag cagtgaaact tttgctaatg atttcatcaa
    19081 ttcgatccaa aacaaaacag cagaagagtt ccgtcaagaa tatcgaaatg ttgatttact
    19141 gctagtggat gacattcaat tttttgcaga aaaagaagct acgcaagaag agtttttcca
    19201 tactttcaat gccttgtaca atgaagggaa acaaatcgta ctaaccagtg atcggttgcc
    19261 aaacgagatt ccgaaactgc aagaacgcct tgtttctcgc tttgcatggg ggctgtctgt
    19321 tgatattaca ccgccggatc ttgaaacaag aacagcgatt ttacgtaaaa aagcggcggc
    19381 tgaacgtcta gagatcccag atgacacact aagctacatc gcaggacaaa ttgattctaa
    19441 cattcgtgaa ttagaaggtg cgcttgttcg agtgcaagct tttgctgcga tgaatagtga
    19501 agatatttcg actagtctag ctgcagacgc actgaaaacg ttaaaatcag gaaaaggaca
    19561 tcctcaattg tcaatcctgc agatccaaga agaagtggca aagtattatc acatccaatt
    19621 gaaagatctc aaaggaaaga aacgcgtcaa atctattgtt gttcctagac aaatcgctat
    19681 gtatttggca cgtgagctca cagataattc tttaccgaaa atcggagctg aattcggtgg
    19741 caaggatcat acaacggtta tccatgctca tgaaaaaatc cagcagctga tggattctag
    19801 tgtcagtatg caaaatgaag tatcagaaat caaaaacttt ttactaaatt aatttgtcga
    19861 ttctatcaac tgaaaatagc atgtggataa aagaaaaaaa cgttcaaaag ttatacacac
    19921 tttttccaca gctgtttttt cgtttctctc ttacgttttt tgagttttcc acagaatcaa
    19981 catgccctat tacttttatt attcttttta actaataata aataaacaaa cattcaaagg
    20041 agatacatca tgaaagttac tttaaaccga gctagcttta tgcaggaatt gcaaactgtt
    20101 caacgagcta tttcaagcaa aaccacgatc cctattttga caggtgtaaa aatcacactg
    20161 acacaagaag gtttgacttt gacggggagc aacgctgata tatcaattga aacttttttg
    20221 tctgttgaaa acgaaaaagc aaatatgcaa atcgaatcta ctggttccat tgttttacaa
    20281 gcacgtttct ttagcgaaat cattcggaga cttcctgaag aaacatttac tttagaagtt
    20341 ttagaaaata aacaagtagc gatcacttct ggaaaagcga attttatcgt aaatggatta
    20401 gatgcagata actatcctca tcttcctgtt gtcgaaagcc ataaccagat gaaattacct
    20461 gtacacgtat tgactaaact aatcaacgaa acagtttttg ctgtctcaca acatgagagt
    20521 cgtccaatct tgacaggtgt gcatttcatt ttatctgata attctttatt agctgtagct
    20581 actgattctc accgtctaag tcaacgcgtg attccagtag aacaagcggc tgatcatttt
    20641 gatattgtta ttcctggaaa aagtttgatc gaattatctc gctcattaac aaatgaagaa
    20701 gaaatcgtcg aaatcagcat tatggaaaac caagtgttgt tcaaaacaga aacgatgtat
    20761 ttctattccc gtttgctaga aggaaactat ccagatacca atcgtttgat tccttcaagt
    20821 tttaatacag aggttgaatt ttctgttcct agctttttag cggcgatcga acgggcttct
    20881 ttactttctc atgaaggccg taacaacatc gttcgtttat ctattcgtcc agatgcggtt
    20941 gttttatacg gaaattcgcc agaaatcggg aaagtagaag aaagcttgag ttatacagca
    21001 agcagtggcg atccattaga tatttctttc aatccggatt atatgaaagc agcgttgcgt
    21061 gcatttggag atatgagcat caaagtgaaa tttatctctg ctattcgtcc atttacattg
    21121 gaaccaacgg aagatggcgt tcaatttatt cagctgatta ctcctgtacg tacaaattaa
    21181 caatgatttt caatgttctt gaaaatttaa ttgaaaagaa atagaaaaaa taaaatttta
    21241 aacagaaggc cctaaaatct cttttaaggc tttctgttta tttttgttta aatcaccttt
    21301 tttagattaa atcgtcttat atggaaaaat atatggataa atttgtttta ttgcgaaaaa
    21361 aaaggtataa tagagtatac ttttaataat gaaaatgagg gatgggcatt gaaacaacaa
    21421 gtcattctaa atacggaatt catgacgttg ggccaaatgt tgaaagaagt cacagttatc
    21481 ggtagtggcg gacaagcaaa atggtatttg gcagaaaaca ctgtgttagt cgatggcgaa
    21541 ccagaaaatc gtcgtggacg caaactttat cccggcatga tggttgaagt tccagaagta
    21601 ggaacttttt ttatggtaaa aaaaggaaac gacgataatg aggctgaata agctgtattt
    21661 aaaaaattat cgaaattacg aagagctgaa catcgaattt tccaagaacc tcgtcatctt
    21721 tttaggagaa aatgcgcaag gaaaaacgaa tcttttggaa agtatctacg tattagctat
    21781 gacgcgaagt catcggacaa gtagtgagca ggaattgatt ggctggactg acgatcaggc
    21841 gatgattcaa ggagagatca caaaaggttc atccactctt ccattagaaa tcttattatc
    21901 taaaaaaggc cgaaaaacca agatcaacca tatcgaacag aaaaagttaa gtacttacgt
    21961 gggacaaatg aatgtcattt tatttgcacc agaagatctt tctttagtaa aaggcagccc
    22021 gcagatcagg cggaagtttt tagatatgga attaggccag atcaatccaa tctatcttta
    22081 tgatcttgtc caataccaag caattttgaa acaaagaaac cagtacttga agcaattgaa
    22141 tgaaaaaaaa caaactgatt tgctttattt ggatattttg tccgaacaac tagcagcgtt
    22201 tggcagcaaa gtattgaaag caagagcaca gttcgtcaag cgattggagt tttgggctaa
    22261 ttctttgcat cagcaaatca cccatcaaaa agagcagctg gaaatcgagt atctcaccgc
    22321 agtcgattct ttagaaacac atacgcaaga gcaaatccaa gaacaatttc tcgcactttt
    22381 gaaccaaaat aaaaagaaag acctttttcg gggaacaacg acagttggtc ctcatcgtga
    22441 cgatctttcc ttttttatta atcagaaaaa tgtgcagact tatggatcgc aaggacagca
    22501 aagaacaact gcactaagtg tcaaattagc agaaatcgat ttgattaaag aagagacagg
    22561 ggaatatcca atccttttgt tagatgatgt gatgagcgag ctggatgata atcgtcaact
    22621 ccatctgcta gaaacaatcg aaggaaaagt tcaaacgttc ttaacaacaa cgacacttga
    22681 ccatgtaaaa aataaaatga ctgttgaacc tgagattttt tatgttcaac aagggaaact
    22741 aaaaggagaa gaaactgaat gacagaagaa agaagtttag tagaacgcgc taaagagtat
    22801 gatgccagtc agattcaagt acttgaagga cttgaagccg ttcgtaaacg acctggcatg
    22861 tatatcggtt cgactagttc tgagggtctg catcaccttg tttgggaaat tgtcgataat
    22921 tcgatcgatg aagtattagc aggttttgca acgaaaatcc atgtgattat tgaaaaagac
    22981 aacagtatca ccgttattga cgacggtcgg gggatacctg tagatatcca ggcaaaaact
    23041 ggacgtcctg cagttgaaac ggtctttact gttttacatg ctggaggaaa attcggcggt
    23101 ggtgggtaca aagtttctgg tggacttcac ggagtaggat cttctgttgt aaacgcgctt
    23161 tctacacagc ttgatgtcaa agtatacaaa gaaggaaatg tgtactatca agaatatcgt
    23221 cgtggagctg tagtggacga cttaaagatt atcgaacaaa cggatcgcca tggaacaacg
    23281 gttcacttta cgccagaccc tgaaattttt acagagacaa ctgagtttga tttttcaaaa
    23341 ttggctaccc ggatccgtga acttgctttc ttgaaccgcg ggatgcgtat atcgattgaa
    23401 gacaaacgag aagaagaacc agtcatcaat gaataccatt atgacggcgg gatcaaaagt
    23461 tatgtcgaat acttgaatgc aaacaaaacc gtgatttttc cagaacctgt atatttggaa
    23521 ggtgaacagc aagatatcgc agttgaagtc tctatgcaat atacagacgg ctaccattcc
    23581 aatatcatga gttttgccaa caatatccat acgtatgaag gtggaacaca tgaatcagga
    23641 ttcaagactg ctttaactcg tgtgataaat gattatgccc gaaaacaaaa gctgatgaaa
    23701 gaaaatgacg acaacttgac cggtgaagac gtgcgagaag gattgacagc tgttatttct
    23761 atcaagcatc ctgaccctca attcgaagga caaacaaaaa caaaattggg caattccgaa
    23821 gtacggacag tcactgatcg gttatttagc gaacatttca tgaaattctt gctggaaaac
    23881 ccaagtgtgg gccggcaaat tgttgaaaaa ggattactag cttccagagc tcgtctagca
    23941 gcgaaacgtg cgcgtgaagt aacgagacgt aaaggtgctt tagagattag taatctgcca
    24001 ggaaaattag cggactgctc tagcaacgat cctgaaaaat gcgaattgtt catcgtcgaa
    24061 ggagattcgg ccggcggatc agctaagcaa ggtcgtaatc gagagtttca agccatcttg
    24121 cctattcgtg ggaaaatctt gaatgttgaa aaagcaagca tggacaagat cttggcaaat
    24181 gaagagatcc ggtctttatt tactgctatg ggtacagggt ttggagaaga ttttgatgta
    24241 tcgaaagctc gctaccacaa attagttatc atgactgatg ccgatgtcga cggcgcgcat
    24301 atccgtacat tgctgctgac tcttttttat cgttacatgc gtcctattgt tgaagctgga
    24361 tacgtctata ttgctcagcc acctttatac ggggtaaaac aagggaagaa tattacgtat
    24421 gtacaaccag gaaaaaatgc agaagaagag ttgaaacaag tcgttgcttc acttcctgcc
    24481 tcaccaaaac caagcgttca gcgttataaa ggacttggag aaatggatga tcatcaacta
    24541 tgggaaacaa ctatggaccc aagcaatcgg atgatggccc gcgtcagtgt ggacgatgct
    24601 attgcagcag atcagatatt tgaaatgttg atgggggatc gagtagaacc ccgtcgtgca
    24661 ttcatcgagg aaaatgcaca ctatgtgaag aacttagata tttaatcatg aaggaggaaa
    24721 aagaatgagt gaagaaatca gagaaaacat ccatgatgtc aatctaacca gcgaaatgaa
    24781 agactccttc atcgattatg cgatgagtgt tattgtcgca cgtgcacttc ctgatgtcag
    24841 agatggtctg aaacccgttc atcgccgtat tttatacggc atgaacgaat taggtgtaac
    24901 accggataaa gcccataaaa aatctgccag aatcgtcgga gatgtcatgg gtaaatacca
    24961 tcctcatgga gacattgcga tctatgagtc aatggtacgg atggcacagc cattcagcta
    25021 ccgttatatg ctagtggatg gtcatggaaa ctttggttct gttgacggtg acggtgccgc
    25081 cgctatgcgt tataccgaag cacggatgag caaaatcgct acagaaatgc tgcgtgatat
    25141 caataagaac actgttgatt tccaaagcaa ctatgatgat acagaaaaag aaccagttgt
    25201 actacctgct cgcttcccga atcttttagt gaacggaaca acggggatcg ccgtaggtat
    25261 ggccacgaat atcccacctc ataacttgtc tgaggtagta gcggctatcg atttgctgat
    25321 ggataatcct gatgtgacga ccaatgaatt gatggaagta ttacctggac cagattttcc
    25381 aacaggtggt ttggttatgg ggaaatcagg aatccgaaga gcgtatgaaa caggtaaggg
    25441 ttctattacc gtacgtgcaa aagttgagat tactgagatg ccaaatggta aagaacggat
    25501 catcgtgaca gaattgcctt atatggtcaa taaagcaaaa ttgatcgaac ggatttctga
    25561 actgcacaga gacaagcgta tcgaagggat cacagatttg cgggacgaat cttcccgtga
    25621 aggtatgcgg atcgtcattg acatccgtcg tgacgccagc gcatccgtta tcttgaataa
    25681 cttatataaa ttgacatcct tgcaaaattc atttggattc aatatgttgg cgatcgaaaa
    25741 aggtgtgccg aaagttttga gcttgaaaca aatccttgaa aactatatcg aacatcaacg
    25801 cgaagtcatc acacgacgga cggtctttga aaaagaaaaa gcagaagcaa gagcacacat
    25861 cttagaaggt ttgcgtattg ctttggatca tatcgatgaa atcattgcga ttatccgcgg
    25921 gtctaaatca gatgacgagg caaaagcaac aatgatcgaa cggttcgatt tatccgatcg
    25981 ccagtctcaa gctattttag atatgcgttt acgccgattg accggtttgg aacgggaaaa
    26041 aatcgaaaat gaataccaag aactcttaaa actgattgaa gatttaactg acattctggc
    26101 aagaccagaa cgtgttacag aaatcatcaa gaatgaatta gcagacttga atcaacgatt
    26161 tggtgataaa cgacgcacag aattactagt cggtgaggtc ttaagtctgg aagatgaaga
    26221 tttaatcgaa gaagaagaaa tcgttattac attgacaaac aatggttaca tcaaacgttt
    26281 ggcaaataat gaattcagag ctcaacgaag gggtggacgt ggtgtccaag gaatgggtat
    26341 ccacaatgac gacttcgtca aaaatctggt ttcttgttct acccatgata cattgttatt
    26401 ctttacgaat aacggaaaag tgtatcgggc aaaaggatac gaaattccag aatacgggcg
    26461 tacagccaaa ggaatcccaa tcatcaattt actaggtatc gattcgagtg aaagcatcca
    26521 ggcaatcatt gcggtgacgg gagaagcaga agaaggccac tatctcttct ttacgacgcg
    26581 tcaaggtaca gtaaaacgta cttctgtcac tgcttttgct aacatccgaa gtaatggtct
    26641 gatcgcaatt ggtttgaaag aagacgatga gctcgtcaat gtgctactga cagatggtca
    26701 atctaatatc attattggaa cacataacgg ctactctgtc actttcaaag aagaagcagt
    26761 ccgcgatatg ggacgtacag cttccggggt cagagggata cgcctgcgtg aggaagatta
    26821 tgtcgttggg gcagccttaa tgaaggaaaa tcaagaagta ttagtgatca cagaaaaagg
    26881 ttacggaaaa cggacaaaag tatcggaata tccagtgaaa ggccgcggag gtaaagggat
    26941 caaaacggcg aacatcactg aaaaaaatgg tccactagct ggattgacca ctgtttctgg
    27001 agatgaagac atcatgctga ttacagataa aggtgtcatt atccgtttca acgtatcgac
    27061 tgtttctcaa actggacgtt caacattagg cgtacgtttg atgaagatgg aagctgatac
    27121 gaaagttgtc acgatggcag cagtcgaacc agaagctgca gaacaaacag ttgaagaaca
    27181 aggatcagaa gaataaccat caagactaag tgtgtaagct agcaagaata accgattaaa
    27241 aaatctggtt attcctgctg gcttttttga ttagttgaat ttctcttaaa atattcgaaa
    27301 ctgttcattc tgtattgctt ttgaccaaaa tagagtgtat aattagagct tgtgagtagt
    27361 ctaattaatt agataactct ccttgctctt aggaacctaa gagccgaaag tccacaggga
    27421 ggtgaatatc aatggaaaac acgaaatatg aaatcatgta cattattcgt ccaaacattg
    27481 atgaagaagc aaaaactgct ttaatcgaac gtttcgatgc aatcttgaaa gataacggag
    27541 cagaagttat cgaatcaaaa gattgggaaa aacgccgttt cgcttatgaa atgaacggtt
    27601 accgtgaagg tatctatcac atcgttaaag tatcttctcc atcatcagca aatgctgtca
    27661 atgaatttga ccgtcttgct aaaatcaacg acgatatcat tcgtcacatg attgttaaag
    27721 aagaagctta attgttgttt catgtgaaac aatagaaaac gatgagagga gatgactatt
    27781 ttgattaata atgttgtatt agttggacgt ctgacgaaag atccagattt gcgttacaca
    27841 gcaagtggta cggcagtcgc aacatttact ttggctgtga atcgtaattt tacaaaccaa
    27901 agcggcaatc gcgaggctga ttttatcaac tgtgtgattt ggcgcaaatc agcagaaact
    27961 ttagcaaact atgctcgtaa aggaacactt ttaggtgtta ctggacgtat ccaaactcgt
    28021 tcttatgata atcagcaagg acaacgtgta tacgtgacag aagtagtagc tgacaacttc
    28081 caattgctgg aaagtcgttc tgcatctgaa aatcgccaac aaagcggcgg attccaaagt
    28141 tctggtcaat cagctggcgg atttggcgga aacaataact ctaatcaaac atctcaatca
    28201 tccaacggta tgcctgattt cgaccgtgat acttctgacc cattcggctc aagttcaacg
    28261 atcgacattt cagacgatga tttaccattc taaacgaatt tgataggagg gaaatagaat
    28321 ggcacaacaa agaagaggcg gacgtaaacg tcgtaaagta gactacattg ctgctaacca
    28381 tattgaatat atcgattata aagatatcga attactaaaa agatttattt cagaacgcgg
    28441 taaaatcttg ccacgtcgtg taactggtac aggcgctaaa aaccaacgta aaattactgt
    28501 tgcaatcaaa cgcgctcgta tcatgggctt gctacctttc gttagcgaag actaataaat
    28561 cctaggaatt gaatgactga tccttttgat cagctattca attccttttt ttctgtaaaa
    28621 ataacctcta ttcgtttagt cgctcgaatg acgaaagtta tggtaaaatc aaattagtca
    28681 tatatgggag gaaaattatg gaaaataaaa acaatcattt atccccgaaa cctctcttcc
    28741 ttatcctgtt tatattaatc gaagtattgg tagtcttaat tttaccattt cgttttgtta
    28801 gcgcttcggt cattctattg atgaatttat ggggtgttcg ctattggttg aaaatgaaac
    28861 gttggcttat tttaaataaa cgtgaaaaaa tccagcaggc ttcgactgta gctgaaacca
    28921 atctagctta tgtctcagaa gtcatgcctg taggagtgat tgcttatcag ccatcaagcc
    28981 aaaaaatcga atggctcaat ccattagcat tgaaaatcaa agatcaaagt ggattaacag
    29041 aggaagaatt gctagatact ttttttactg caaaagaaaa aaagaaaaac caagtctatc
    29101 tagcagataa aacatttcag tttgatatgg atacagtaaa gggtgttgtt tattttaatg
    29161 atattaccca aaaccagttg ttaaaactcc atcagacgaa cagtcagccg gtgattggta
    29221 ttttatctat tgataactat gcagatgcca ttgataaatt agatgacaaa gagatttctt
    29281 atttgaactc tttcgtcact acactagttt ctgactggat gaatgaatac catgtctttt
    29341 tcaaaagaat caacgcagaa cggtttttct tcgtagcaca gatgatcgat ctgaaaaaga
    29401 tgatggataa aaaatttgat ttgctggatc gctttcgagc agaagctgaa aaacagagct
    29461 cgcctatcac ccttagtatg gggattgctt atggctcaag aaccctcgat gaaatcggtg
    29521 gagaagctca gaataattta gatgttgctt tagtccgtgg aggcgaccaa gttgtcgtga
    29581 aagaggtaaa agaaggagca aaacctgttt attatggggg gaaatctgct aagaatgtca
    29641 aacgcacgcg tgttcgttct cgagcgatga gtaccgcttt gcgtcggatt tttgatgaaa
    29701 caggcgatgt gtttatcatg gggcacaagt ttccggatat ggatgcttta ggctctgctt
    29761 ttggtgtcag ttgtctggca cgcttcaacc agaaaaaagc ttacatcgtt atcaatgaga
    29821 acgagatcat tccagatgta gaacggtgtc ttgaagaaat ccataagcat cctgaattag
    29881 aaatgcaact aatctctcca gaacgtgcca tagagctgaa aaaagaagag gacctcctcg
    29941 tgatggtgga ttaccataaa ccttctctat cgatctctca gggcctatat gagcaatttg
    30001 ataagattgt gatcattgat caccatcgtc gtggggaaga gtttccaagt aaacctctgc
    30061 tgacttatat tgaatcttca gcttcttcag cttcagagct agtttctgag ctgatccaat
    30121 accaaagcag caaaacaaat cgcttagatc gttttgaggc cacattattg cttgcaggga
    30181 tggttgtaga tacaaatagt ttcaatgtcc gtacaactgc acgaactttt gacgttgcca
    30241 gctatttaag gtcttgtgga gcagacgcat ccttagtaca atatttattg agctcggatt
    30301 tagcgacata cttggagatc agcaatttga ttgctggaag cgactatgtg acttctgata
    30361 ttgttgtggc ggcagggaca gacgaaaaag agtataatag tgttatagca gcaaaaacag
    30421 cagatacatt gctttcgatg atcaatatca atgcagcttt cgtcattaca aagcggacag
    30481 acggcttgat tgggatcagt gccagaagta ccggcacgat caacgtccaa atcatcatgg
    30541 aatccttagg tggcggagga tacttcacaa acgcagctac tcaactggaa aatacgacag
    30601 ttgaggaaac aaaacagaag ctatttgctg caatcaaaga aaatatgaat caaatatatg
    30661 ataaggagta atccaaatga aagtaatttt tttacaagat gtaaaaggaa aaggtaaaaa
    30721 aggggacatc aaagaagttc cagcaggtta tgcacaaaat tatctaatca aaaataactt
    30781 ggcaaaagaa gcaacgaaaa gcagtatcag tcagttagca ggacaaaaga aagcacaaga
    30841 aaaacacgaa gctgaaatct tggaagaagc taaaaagtta aaagaattct tggaaaaaga
    30901 agatactgta gtcgaattga aggcgaaagc aggggaagac agccgtttgt ttggttcgat
    30961 cccttcaaaa caaattgctg aagggttaaa caaacaatac aaaatcaaat tagataaacg
    31021 aaaaatcgaa ttagaaaatc cgatccgttc actaggctac acaaacgtac ctgttaagct
    31081 tcatcatgag gtgacggcta cgatcaaagt tcatgtcgta caagaataac aaatcttgat
    31141 agcgatgttc agctggtaag tataaccaga aggaaaaaag acaaaccgaa atttatctga
    31201 gaaaacagct gcagcaatga aagcaaaagc tgcaaatcga tttcagattg atttcggttt
    31261 cttttttggg aacagcaaaa tagcataaag aaaacgagta taataaaaga gaactatttt
    31321 tttattacac tttcggtacg aatgatgact tttctctcat tttcttgtaa aatgaagcaa
    31381 ttgaagacaa ggagcgatac gtatgaatga agtgtggcag gatcgaatcc caccgcaaaa
    31441 tattgaagct gaacaagcgg ttttaggctc tgtttttctt gacgcagaaa cgatcattga
    31501 tgccatggag tacatagagc caaaagactt ttatcgtcgt ggccatcaaa ttatttttga
    31561 gacaatgatc gagttaaatg atcgtaatga agcgattgac gtggttactg tcaaagaccg
    31621 gctggaacaa gccaatttgt tagaagatgc cggtggttta agttatctct cagacttggc
    31681 attagcagtt ccgacagcag caaatatcgt ctactatgcg aaaatcgttg aagaaaaatc
    31741 attgctgcgt acattgatcc agacagcaac aggaatcgtt acgaaaggat tcgagcaagg
    31801 agaagaagtt caaacaattt tagatgatgc cgaacgcagt atcttggagg tttcggaaaa
    31861 aagaaatcga agcggctttt tatctattgc tgaagtactg aactccacga ttgaaaatat
    31921 cgatcgacta gctcaaaaca atgaagaaat cacaggtcta ccaactggct atcaaggtct
    31981 ggataaaatg acagcaggtt tccagcccga agaactaatc attcttgctg cacgtccagc
    32041 ggtaggtaaa acagcgtttg cattgaatat tgctcaaaat gtaggaacaa aaacagatcg
    32101 ttctgttgcg atatttagtt tggaaatggg ggcagaatct cttgtaaatc ggatgctttg
    32161 tgcagaaggt tcgattgagg ccagccattt acgtacaggt cagctttcgg aagaagaatg
    32221 gtcgaatctg atcgttgcga tggggagtct ttctcgagca aatattttta tcgatgatac
    32281 accagggatc aaaatctctg aaatccgtgc gaaatgtcgg aaactagcgc aagaaaaagg
    32341 caatctaggc ttgatattga tcgattattt gcagttgatt gaaggtaacg gtaaagagaa
    32401 ccgtcaacaa gaagtatcag atatctctcg ccaactgaaa aaactggcga aagagctgaa
    32461 agtacctgtc attgctcttt cacagttatc tcgtggagta gaacagcgac aagacaaacg
    32521 accagtgcta agtgatattc gtgaatccgg atcaattgaa caagatgcgg acatcgtagc
    32581 cttcttgtat cgagatgatt attatgaaag agaaggcgga gatgacgatg atggaaacga
    32641 gccaaaaaat gacaacgtca tcgaagtcat catcgagaag aaccgaagcg gtgcacgagg
    32701 aacagttgaa ctactgttta tcaaagaata caataagttt tcgtcgcttt cgccaagaga
    32761 agaattttaa agaagaggtt gtgagccttg agcaatcccg agagctctcg taagaagatg
    32821 caaggagtaa ccgcaggaac gactagagta cttaatttaa acagatctcc aaagggattg
    32881 tgacaaaagt cttgtcacag tccctttttc cgaataaacg gtgttcaatc agctgaccct
    32941 tggtattagc tcagcaaacg tcaaaatatg aggaactatt ttgacgtttg tttctctaat
    33001 actcaggtca aaacgctgat cttacgacct cttttcctta aacggtgttc tcctgccaac
    33061 tcctcggtca caagtcacaa tatcatcaaa catgagaagc agttttttga aattgctcct
    33121 tgtgactcag agttaaacgg caggctcaca acctctttct taaacggtgt tcaaaagctt
    33181 gcatctgcgg taatacgccc aatcaaacga aaaatatgag aagctatttt tgtttggctg
    33241 ttcttattac ttgatgcagg acagcttttt cacaacctct gattcacggt gttccaaaag
    33301 tagcttcttc gaaaaataag tcgaaatttc acaaaaattt gagaagcaat tttcgtacat
    33361 tccttcttat ttcttgaagc tgaccacttc tgttacaacc tcttttttaa taaccattct
    33421 aaaaagataa agttcgtttt ttaagcgttt ttataaaata aaaataaaaa tcattcgatt
    33481 ttatttgaaa tatgtatcta ttattgatac aatggttcag gattaaataa tatagaacga
    33541 ggtgttcaca tgtcatctgt agtagtagta ggtacgcaat ggggcgatga agggaaagga
    33601 aaaatcacag actttctcag tgaaaatgct gaagtgattg cacggtacca aggtggcgac
    33661 aatgcagggc acacaattaa gtttaacggt gtgacttata aactgcattt gattccttct
    33721 gggatttttt ataaagacaa gatcagtgtg atcggaaacg gcgttgtagt caatcctaaa
    33781 tcattggtta aagaattagc ttatttacat gaaaaaggtg tagaaacaag taatttgcgt
    33841 atttctgacc gcgcgcacgt aatcttgcct tatcatatca aattggacca attacaagaa
    33901 gatgctaagg gcgaaaataa gattggtaca acgattaaag gaatcggacc tgcctatatg
    33961 gataaagcag ctcgtgtagg tatccgtatt gcagacttat tagataaaga aatttttgaa
    34021 gaacgtcttc gtttgaatct agatgacaaa aaccgtcaat ttgtaaaaat gtttgattca
    34081 gaaccacttg attttgacga tatttttgaa gaatattatg aatacggaca acaaatcaaa
    34141 caatacgtca cagatacttc cgttatcttg aatgatgctt tggatgcagg caaacgcgtg
    34201 ttgtttgaag gcgcacaagg tgtaatgctg gatatcgacc agggaactta tccttttgtt
    34261 acttcttcta acccagtagc cggtggggta acaatcggaa gcggtgtagg gccttcaaaa
    34321 atcaataaag ttgtcggcgt ctgcaaagcc tatacgtcac gtgtcggtga tggaccattt
    34381 ccaactgaat tatttgatga aacaggagaa caaatccgcg aagtaggacg agaatacggt
    34441 acgacaactg gtcgtccgcg tcgtgtaggc tggtttgatt ctgtagttat gcgccattct
    34501 aaacgagttt caggtattac caacctatct ttaaactcga tcgatgtatt aagtggattg
    34561 gaaacagtaa aaatttgtac ggcttatgaa ttagatggcg aactaatcta ccactatcca
    34621 gcaagcttga aagagttgaa ccgctgcaaa ccaatctatg aagaattgcc aggctggtca
    34681 gaagacatta caggatgcaa aacacttgca gaactgccag aaaacgcacg taactatgta
    34741 cgtcgtatct cagagcttgt tggtgtacgt atctcaacat tttctgtcgg acctgataga
    34801 acacaaacaa acatcttaga aagcgtctgg gcgcaaatct aaggataaaa acgacgactg
    34861 aatcaggacg ttcttaccag agcgagtcga agtgtttatc tatacgataa taataacttg
    34921 aagaggtagt gaaagagacg tttagcctta ttgccgatag gctgtctctt gaacactgtt
    34981 tattcagaat aagggactgt gacaaaaacc gtgtcacagt ccctttttcc gaa
//
LOCUS       DASNNU010000021        33819 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.021, whole genome shotgun sequence.
ACCESSION   DASNNU010000021 DASNNU010000000
VERSION     DASNNU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.021"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(99..1229)
                     /locus_tag="U8116_000962"
     CDS             complement(99..1229)
                     /locus_tag="U8116_000962"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEN8433972.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="U8116_000963"
     CDS             complement(1878..3080)
                     /locus_tag="U8116_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN8433973.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="U8116_000964"
     CDS             complement(3140..3343)
                     /locus_tag="U8116_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEN8433974.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="U8116_000965"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="U8116_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="U8116_000966"
     CDS             4880..5692
                     /locus_tag="U8116_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8433975.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="U8116_000967"
     CDS             complement(6716..6868)
                     /locus_tag="U8116_000967"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN8433976.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="U8116_000968"
     CDS             complement(7059..7937)
                     /locus_tag="U8116_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN8433977.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="U8116_000969"
     CDS             complement(7976..9094)
                     /locus_tag="U8116_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEN8433978.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="U8116_000970"
     CDS             complement(9091..11190)
                     /locus_tag="U8116_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEN8433979.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="U8116_000971"
     CDS             complement(11241..12062)
                     /locus_tag="U8116_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN8433980.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="U8116_000972"
     CDS             complement(12059..12868)
                     /locus_tag="U8116_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN8433981.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="U8116_000973"
     CDS             complement(12889..13383)
                     /locus_tag="U8116_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN8433982.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="U8116_000974"
     CDS             complement(13395..13883)
                     /locus_tag="U8116_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN8433983.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="U8116_000975"
     CDS             complement(13960..16656)
                     /locus_tag="U8116_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN8433984.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="U8116_000976"
     CDS             complement(17084..18157)
                     /locus_tag="U8116_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN8433985.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="U8116_000977"
     CDS             complement(18154..18981)
                     /locus_tag="U8116_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433986.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="U8116_000978"
     CDS             complement(18982..19788)
                     /locus_tag="U8116_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8433987.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="U8116_000979"
     CDS             complement(19800..20885)
                     /locus_tag="U8116_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8433988.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="U8116_000980"
     CDS             complement(20902..21444)
                     /locus_tag="U8116_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8433989.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="U8116_000981"
     CDS             21702..22691
                     /locus_tag="U8116_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN8433990.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="U8116_000982"
     CDS             22688..23425
                     /locus_tag="U8116_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEN8433991.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="U8116_000983"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="U8116_000983"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEN8433992.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="U8116_000984"
     CDS             23989..24621
                     /locus_tag="U8116_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN8433993.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="U8116_000985"
     CDS             24706..25353
                     /locus_tag="U8116_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN8433994.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="U8116_000986"
     CDS             25358..26341
                     /locus_tag="U8116_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEN8433995.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26744..28258)
                     /locus_tag="U8116_000987"
     CDS             complement(26744..28258)
                     /locus_tag="U8116_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEN8433996.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..29202
                     /locus_tag="U8116_000988"
     CDS             28396..29202
                     /locus_tag="U8116_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8433997.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29377..29946)
                     /locus_tag="U8116_000989"
     CDS             complement(29377..29946)
                     /locus_tag="U8116_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8433998.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30029..32089)
                     /locus_tag="U8116_000990"
     CDS             complement(30029..32089)
                     /locus_tag="U8116_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEN8433999.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32366..>33819)
                     /locus_tag="U8116_000991"
     CDS             complement(32366..>33819)
                     /locus_tag="U8116_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8434000.1"
                     /translation="EDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFKTRNYIIELN
                     KEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTEIVDSKVTIE
                     KFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFGVYFGVFNGF
                     SMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGNQPIGKRFFS
                     DEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELDGAFGLAEAH
                     FHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNWIFDFEMTSF
                     TSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFLLVGICPVSY
                     FEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSDCLLEKFNKE
                     QIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT        10162 a         7030 c         5707 g        10920 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttaccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccacctt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat aaaacatgct aaaaaacaag
    29221 agaaggggca gtgacaatac ttttgtcact gccccttctt ttttgaatgt atggcatgcc
    29281 attagttatc cgatagtatt aactcaaaaa agtaagatat gaggaatgac tatttcgttt
    29341 tttatcccaa tactgaggac ttatttcacc atttcgttat ttatctaatt tacttaaaac
    29401 ctgcataact ttttcataat ctggattagg tgtgccgtct agattttcag caataaaagt
    29461 aaaacccatt tttttataaa gagcaatcgc ctgatggctc catgtctgtg tgtgaagata
    29521 gattggtccg tccgtatgga gggtaggaaa ttcttgcaag actctggcag tcaatgcttt
    29581 tgcgagccct tttccttggt aatctggttt cactgctaac caatgaaaaa ccggatacac
    29641 ttgctctctt gatttttttc gccaagcggt acaggttgct actttttctc ctttttttgt
    29701 ctctacaaaa aacatccttc tttcaagttc ttcaggataa ggagcaaagt tttgttgaaa
    29761 atactcaagt gcttcatctg tcgtttgaaa ttccccaacg gaagtctcaa tctccgccca
    29821 agcagattca tcttcttttt gataaaatcg aaattcaaaa ccttccgcta aaggcgtgat
    29881 tggaacattt tgttgaagtg gtcttgtcat ccagatttct gcgtaaggta acgttttatc
    29941 taacattttt ctatccctcc tcgtctccat tatgccaaat aacatatgac tcagctactt
    30001 atttgactat aacagataga gatataaatc agaaccagtc atctgcgtca aattcatttc
    30061 gattagcgta tacccatgtt cctgccatac ctcgcatgaa atctcctaaa gcagaagaag
    30121 gaatacctcc accaggtgcg ggtgttcccg gactaggaag tggtacttgc ggctgcagat
    30181 gctcatcggt ggaatcaata gttgacaagc gttttttctt cgtttccttc tggtcatctg
    30241 attccttctg atcttccgac tgtttagcaa caaattcttt ttctgattgt tgttttattt
    30301 taggctctaa aatttcattt tccaatccag aaacttgtgg ggccaaatga gcttcttgcc
    30361 ttatcgtttt attagattct gagatttttc tagatattct tgctaactct aggcttcttg
    30421 tttgttttac tttccctgtt ggagaaactg catgagccat ttgttgtttt ttttctgaca
    30481 agctttcttc ttgtcttttt tgttcttttt gatctatctg tttgtttaat ggtaaaacat
    30541 gtaatagtga ctgagcatgg agttcttgga gtgaatacgg gtcatgataa ctatacataa
    30601 tcgtaaaatc acctgtttgt ttggtcaaat aaccatcctc caatggtact tcattgattg
    30661 taaccgatac atcctttaac ggtacgggtt tacctaagga atttgttgct gattggatca
    30721 actcttcttt gtttattttt tcgtttggat gaacaataag atcatgacct tctaatgaac
    30781 gattgtctgg tatataaaca taagtaaagg taatttcccc tcctgattgc ttcctattat
    30841 tcatcagttc tgagtgaagt acttcaagga tctccgacca gtgacttttt ctgtctaatc
    30901 cgtttgcttg catccaatca gaaatagaag tgactttccc attttgttca taagtcgata
    30961 aatctatatt tggcactcga tcagaaaatt cagcaggtag gcgcaaatca ggaatacgat
    31021 ttacactact catatgtgtt tctgcttgaa gttctttatt tgtttgacta tctataaaat
    31081 gagtgatgac agtcgatgaa gcagcagatg cttctacata aactacagct gttgtttctg
    31141 ccacatttga tgttttcgag tctatataag ttaatacaac gggatattct cccggagttg
    31201 tagtatctac tgtcccgttc attttgattt cttctttatt tcctgaagtt ccatcgcttt
    31261 ttgttacata ttcaacaaga tctttagggg cgtaactctc tcctgttctt aagtgaagat
    31321 cttttccttt gatagctgtt tgatcagcac gaacatggat cacagcagat gcagtcattg
    31381 tttttaaagc aattggatca aaataggaca aggaaatagt agatgttcct gtattagtcg
    31441 tatccacgat attcccattg tttgaaatta ctttggacat atctccttgt gttccatctg
    31501 cgttcttcag cctttctacc agatcagata tcgaaacttt tgtccctgcc agaacatcga
    31561 ttttctttct ttcaaactgt gaataatctt tttcatatac ataagaaact tgtgcgcttc
    31621 caccgtcctt tatatctgtc aaagcatgat tcaaaatatc agtgatctgt tttaactgat
    31681 caccttttgt aggatcagtc atatcggaaa agatagaaag cggcacactg cttccggttt
    31741 ccgatgtcca taatgttttg ttaggaatta accagtagtg gtttatcgtt ggaggataac
    31801 tgactttatt ataggaagtc aatgcaggaa gtagctgttt tccatttgca tcagtgaaag
    31861 tatgagtaat agtgataggg gaatcagtgg tctcagaaac agaggtacgt acgaccttga
    31921 tggtttgtgt cgttgcctga acgggtacat gcatatagac acctacagat aaactgatca
    31981 gcatcgtgcg catcataaac ttaacgtagt gtttttcaga acacgaccgt tccttatcta
    32041 aacgacaagc gtgtttattt agttgttttg aacgtctttt tgaacacatt tattcttagt
    32101 cctccctact cttgtctaaa catgaagtag taattttcat aagatgattt cattatataa
    32161 ataaaatcaa aagaagatgg actattagga gagtattttt tttgattttt tattgtttca
    32221 agaaaatcgg aataataact tatgtacttt ttaattttgt taagtaatat atctagttat
    32281 atctagttat atctagttac cttttctttg aaaaaaacat aaatcagcta ataaatatgt
    32341 gagacaaagc tgccatccat aaatactagc tgattttttc agttaaatgt ctattttttt
    32401 ctctgatgat ggtatttcca aaatattccc agtcatctgg agtcggtgga tttttccaaa
    32461 ttatctgttc tttattaaat ttttcaagta aacaatcaga taaataaata ttcgtacgag
    32521 aagtgattcg actctcaatc acaatattgt aatttttaaa tcctttcact tgagaaataa
    32581 tgtaatcggt ataagcagat ccttgcgaaa aatcaataca gacatgaatc ggtgcttcaa
    32641 aatatgatac cggacaaata ccgaccaata aaaagagata atcataaaat aagcgaatca
    32701 tgaaacgttc atcttttccc aagaaatagc gtttattctc ttgtatgacc ttttggattg
    32761 ctgtactgaa tattggatac gtctcgaaga aaaactgtag ctgcttttta gacgtaaagc
    32821 tagtcatctc aaagtcgaat atccaatttt ttcgattgac atgcatcata cccatacgaa
    32881 gttcttttcg tatatatagt ctttcttctt cagagatttc aatcgaaaat ggtaattgat
    32941 caatcaattc cactgacatt ttttgactaa tcatgtggat atcatgaaaa tgagcttccg
    33001 caagtccaaa tgcgccatct aattcaaatt cttcttccgt gatcaaaaac aacatgatat
    33061 aagaaatctc atcaatgatc tgctctttag ggatatgaag aaaagaaata tgtgataaat
    33121 ccttgatgat cttcattcct tctgagctct taaagtattc atctgaaaag aaacgttttc
    33181 cgattggttg attcccatag gccctcaaca aactaatagt ggttagcaaa ttgaatttga
    33241 ttcgttttgt tgcagacagt tttaaattga acagatattc aaaatattgc acgatactct
    33301 ttgcttcatt catgacgctt tctctaaatg gcatagaaaa accattaaag accccgaaat
    33361 ataccccaaa taatacgttc cgtatgttta tttcttcccc aactaatatg tttttcttta
    33421 actttatttg atgctgttca agaaaagaga ctaattctct tctttttaca taggctcttg
    33481 atatgcttaa aaaatgattc tctgcaaatt tttcgatcgt tacttttgaa tccacgatct
    33541 ccgttaacag tcgagcaatt tttgatcgtt gaaagtagat tcttcttaaa tttttgatat
    33601 ccgtcaaatt cagacgttcg acatggatct ctgcttcttc tgaaatttgg atctctggct
    33661 ttgttttgaa ttcagtaaat tctttattta attctatgat ataatttctt gttttaaatt
    33721 tagacaaacc taatgtttca tataacttag gcagcactac ataattcttt ttctcaaatt
    33781 ctaaatatct agctagcagc agtttttctc tatcttcct
//
LOCUS       DASNNU010000022        32964 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.022, whole genome shotgun sequence.
ACCESSION   DASNNU010000022 DASNNU010000000
VERSION     DASNNU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.022"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..162
                     /locus_tag="U8116_000992"
     CDS             <1..162
                     /locus_tag="U8116_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434001.1"
                     /translation="KYRIPIYVIEDYIWKLKGTRYREEEIIATLIYSVTDRHWQLVED
                     ADLSYLPLK"
     gene            complement(243..812)
                     /gene="thiT"
                     /locus_tag="U8116_000993"
     CDS             complement(243..812)
                     /gene="thiT"
                     /locus_tag="U8116_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEN8434002.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(878..980)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(986..1957)
                     /locus_tag="U8116_000994"
     CDS             complement(986..1957)
                     /locus_tag="U8116_000994"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN8434003.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2051..3424)
                     /gene="glmU"
                     /locus_tag="U8116_000995"
     CDS             complement(2051..3424)
                     /gene="glmU"
                     /locus_tag="U8116_000995"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEN8434004.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(3927..4745)
                     /gene="purR"
                     /locus_tag="U8116_000996"
     CDS             complement(3927..4745)
                     /gene="purR"
                     /locus_tag="U8116_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEN8434005.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(4912..5736)
                     /locus_tag="U8116_000997"
     CDS             complement(4912..5736)
                     /locus_tag="U8116_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN8434006.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5709..6389)
                     /locus_tag="U8116_000998"
     CDS             complement(5709..6389)
                     /locus_tag="U8116_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN8434007.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6412..7350)
                     /locus_tag="U8116_000999"
     CDS             complement(6412..7350)
                     /locus_tag="U8116_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN8434008.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(7595..8458)
                     /locus_tag="U8116_001000"
     CDS             complement(7595..8458)
                     /locus_tag="U8116_001000"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEN8434009.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(8600..11173)
                     /gene="mprF"
                     /locus_tag="U8116_001001"
     CDS             complement(8600..11173)
                     /gene="mprF"
                     /locus_tag="U8116_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN8434010.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11314..12120
                     /gene="proC"
                     /locus_tag="U8116_001002"
     CDS             11314..12120
                     /gene="proC"
                     /locus_tag="U8116_001002"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEN8434011.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12199..13755)
                     /gene="rny"
                     /locus_tag="U8116_001003"
     CDS             complement(12199..13755)
                     /gene="rny"
                     /locus_tag="U8116_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEN8434012.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(14166..15215)
                     /gene="recA"
                     /locus_tag="U8116_001004"
     CDS             complement(14166..15215)
                     /gene="recA"
                     /locus_tag="U8116_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEN8434013.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(15324..16565)
                     /locus_tag="U8116_001005"
     CDS             complement(15324..16565)
                     /locus_tag="U8116_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEN8434014.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(16805..17383)
                     /gene="pgsA"
                     /locus_tag="U8116_001006"
     CDS             complement(16805..17383)
                     /gene="pgsA"
                     /locus_tag="U8116_001006"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEN8434015.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(17592..18515)
                     /locus_tag="U8116_001007"
     CDS             complement(17592..18515)
                     /locus_tag="U8116_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8434016.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(19254..20540)
                     /locus_tag="U8116_001008"
     CDS             complement(19254..20540)
                     /locus_tag="U8116_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN8434017.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(20533..21801)
                     /locus_tag="U8116_001009"
     CDS             complement(20533..21801)
                     /locus_tag="U8116_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN8434018.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            22053..22514
                     /gene="mscL"
                     /locus_tag="U8116_001010"
     CDS             22053..22514
                     /gene="mscL"
                     /locus_tag="U8116_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEN8434019.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(22573..23019)
                     /locus_tag="U8116_001011"
     CDS             complement(22573..23019)
                     /locus_tag="U8116_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8434020.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(23112..23357)
                     /locus_tag="U8116_001012"
     CDS             complement(23112..23357)
                     /locus_tag="U8116_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEN8434021.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(23472..24050)
                     /locus_tag="U8116_001013"
     CDS             complement(23472..24050)
                     /locus_tag="U8116_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN8434022.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(24127..24657)
                     /locus_tag="U8116_001014"
     CDS             complement(24127..24657)
                     /locus_tag="U8116_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEN8434023.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(24661..25521)
                     /gene="rlmB"
                     /locus_tag="U8116_001015"
     CDS             complement(24661..25521)
                     /gene="rlmB"
                     /locus_tag="U8116_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEN8434024.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(25514..25909)
                     /locus_tag="U8116_001016"
     CDS             complement(25514..25909)
                     /locus_tag="U8116_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEN8434025.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(25906..27318)
                     /locus_tag="U8116_001017"
     CDS             complement(25906..27318)
                     /locus_tag="U8116_001017"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEN8434026.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(27315..27851)
                     /gene="cysE"
                     /locus_tag="U8116_001018"
     CDS             complement(27315..27851)
                     /gene="cysE"
                     /locus_tag="U8116_001018"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEN8434027.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(27937..28176)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28173..29633)
                     /locus_tag="U8116_001019"
     CDS             complement(28173..29633)
                     /locus_tag="U8116_001019"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEN8434028.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(29803..30960)
                     /locus_tag="U8116_001020"
     CDS             complement(29803..30960)
                     /locus_tag="U8116_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEN8434029.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(30994..32364)
                     /gene="radA"
                     /locus_tag="U8116_001021"
     CDS             complement(30994..32364)
                     /gene="radA"
                     /locus_tag="U8116_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEN8434030.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(32427..32954)
                     /locus_tag="U8116_001022"
     CDS             complement(32427..32954)
                     /locus_tag="U8116_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEN8434031.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         9072 a         7264 c         5819 g        10809 t
ORIGIN      
        1 aaatatcgta tcccaatcta tgtgattgaa gattatattt ggaaattaaa aggcacccgg
       61 tatcgagaag aggaaatcat cgctacttta atctactctg tcaccgatcg acactggcaa
      121 ttggtagaag atgccgatct ttcttatctg ccattgaagt agatctattt cttatcaaaa
      181 aataaaaagg aaacaagaaa ctaggacatg attctgtctc aagtttcttg cttctttttt
      241 atttacagct cctgtcgttt atctggcaca aaaatcgaag gagtcgtttg ttggatccct
      301 aataaaacga tgatcgttac aatagctgta ctcaacccgc tggcaccatt cattacgaaa
      361 gaaaagagcc aaggattcat cccccatagt gcatacgctc cccagaaaac cactcctgca
      421 ataaaatgcc aaaaatatct ggaaagtgcg ccaatcaatg caccaaacac gatgcttttt
      481 ctaatttgtt tcgtattatc gtctacatag cctttttgga catttgaagc catgagccct
      541 gctgcgcctg caaaagtata ggcaatcggg tactcaatca acacttgaac gacacttaaa
      601 taataaactt gtcccaacgg aaaatgtagg atgccccaaa tgaaacctgc cagcatccct
      661 gcctttgtcc ctcttcttaa cgcaaaaaga gtcaatggaa tctgccctaa tgaaatagaa
      721 aaactgctgc cgatctggat cggaatcaac gacaacacca tcgctaacgc tgcaacaatc
      781 gttccttcta cccaaactct attttttccc ataaaaaaac ttcctccttt gaataaaaaa
      841 gtcacaaagg aggaagcagc tttcgaaaat agtcaaacag ctgtttattc acaattccta
      901 cgctcgcata accgatacag gtacgaaggg tttagaaata gattcaatct cagcccaatt
      961 ggactcccct ttgtgagttt atttattatt tattttttgt ttcaaacaat ggactaactg
     1021 gtttgttttc atggatacgt ttgattgcat ctcccattag tccgccaaca cttacttcat
     1081 cgattttgtc gattttacga tcttctggaa gatagatcga gtctgtaaca actagacgct
     1141 cgatagctga atcttctatt ctttgtaaag cagggccgga taatactgga tgtgtacatg
     1201 aagcatacac actagttgca ccagcttctt tcaaagcatt ggcagccaat gtgatcgtac
     1261 ctgctgtatc gatcatatca tcgatcagta cacaagtttt gcctttgact tgtccaatga
     1321 tattcattac ttctgcaaca tttgcttttg gacggcgttt gtctatgatc gcaatcggag
     1381 atttcaaaaa ttccgctaat ttacgtgccc gtgtgacacc accatggtct ggtgaaacaa
     1441 cgacaacatc atcgcctttg attccttttt ctaagaaata attggcaata agtggcgcac
     1501 ccatcaaatg atcgactgga atatcaaaga aaccttggat ttggactgca tgaaggtcca
     1561 aagtcagcat tcttgttgca ccagcttttt gaatcatgtt tgctactaat ttcgctgtga
     1621 ttggttcccg tgcacgtgct ttacggtctt gacgcgcata gccataataa ggcatgacta
     1681 cgttgattgt ttttgcactt gctcgtttca gtgcgtcaat catgatcagc aactccatca
     1741 agttatcatt gactgggctg ctcgttgatt gaataacata gacatgcgat ccacgaatac
     1801 tttcttcaat gtttacttga atttctccat cactaaattg agtaacagat gattttccta
     1861 actcgacgcc gactgctgct gcaattttct ctgctaatgg gcgattggaa tttaacgcaa
     1921 aaatttttaa tcttgggtca aagtaatgtt ttgacatggg gacctccact ttctttttca
     1981 caactctaga attctaccta ataaatagta gtcatatttt ttaaataaat caagtgattt
     2041 tgcaaaagtc ttagtttaga taagggagtt tttttgcata tccttcttta ttgacttgtc
     2101 ttgctctcgc aattgccagc gcatattctg gaatattgtc tgtaattgtt gacccggcag
     2161 caatcgaact gtttttagct acctcgacag gtccgatgat attcgtgcta gaaccaataa
     2221 agctgtggtc gccgacagtc gtatgatgtt tgttctttcc atcatagttg acgaagacaa
     2281 caccacatcc gacattgatt tcttctccca gcgtagcatc accaacataa gtcaagtgac
     2341 cgactttagt tcctttgccg atttgagcat ttttcacttc cacaaagttt ccgatatgga
     2401 cgttttcgcc aatttcagct tttggtcgta aatgtgcata aggtcccaca tctgcatgtt
     2461 tcttgacatg gctgctttca atgactgagt tttcaatgac gacgtgatcc tcaatcctac
     2521 tgtctacgat ttttgaatgt gacccgatga cacaatcaga accaatgact gtatttcctt
     2581 ggatttgtac acctgcttcg atcaatgtgt ctggaccgat ttctacgcct gcatcgatgt
     2641 atgtcgttgc tgaatcgaca aagcttacgc cattgaccat atgcatctgg ttaattcgct
     2701 ttctcatgat ctcatttgct ttagctaacg cgatccgatc attgactccc attgattctt
     2761 caaaatcatc cgtttgataa gcagcaactg tatggccttc ttcttttaag atttcgatga
     2821 tatctgttag atagtattca ccttgagcat tgtttgttcc aactttgctt aacgcatcga
     2881 acaaagcttc gttgtcaaaa cagtaagttc ctgtattgat ttcttgtaca agagcttctt
     2941 ctggtgttgc atctttttgt tccacgattt tttctacgat cccaatatgg tcacggatga
     3001 ttcgtccata tcctgttggg ttttctgctt gtgccgtcaa gatcgttgca cttgcatttt
     3061 ttccttggtg atattcaaaa agattgttca gcgtttcagt agtcaataat ggtgtatcgc
     3121 cactgataac taacgttgtt cctttttttc cttccaagaa agaagcagct tgcaaaacag
     3181 catgtcctgt tcccaattgt tctgcttgta acgcatattt actgcgttct cctagttgtg
     3241 ctttaacttg ttctgctccg tgtccaacga tcgtaatcac ttcatctggt ttcgtttcac
     3301 tgacccgatt aatgatatgt tctaccatag gtttaccaga aacgggatgt aaaactttat
     3361 ataattttga cttcatgcgg gtacctttcc ctgccgccaa aataattgca taacgtgcgt
     3421 ccaactgcgt cactcctaaa tatagtttgc tgatttcagt ttatcccagt atacccattt
     3481 tttcaacaaa acaagtgatt ttcttttatc ccagcgtttt ctggataaaa aaatagatag
     3541 accataacgg gcctatctat ttggtaaccg gtgctctcct gccaactcct cggtcacaag
     3601 tcacactact cataaaacat gaaaagctgt tttttgaaat cgtttcttgt gacttagagt
     3661 taaacggtag gcttacaacc tcttcattaa acgtttttca atcagctgac cttcggtatt
     3721 agctcaataa acggcaaaat atgaggagca atttttccgt ttattgagct aatactcagg
     3781 acaggacgct gatttcacaa cctctagata aacggtgttc aaaagccagt ttctcggtat
     3841 taagccaaat ttatgaaaaa atgtggaaag ccattttcac aaaatttttc ttactactcg
     3901 aaactaagcg gctttttcac aacctcttat tctgaaaaat aatttcctgg cacaaccgta
     3961 atatttttcg tttgagtatc tacatctttg acataaagca gtgacgtata atcatcgatg
     4021 gcgcgatgac cgttgaattt tgattcggca aatacagtga tgccaacaag ttctgattcg
     4081 aattcttcaa tcaagctctt cattccgttg actgttccgc cgcctttcat gaaatcatcc
     4141 actacaagga cacgtgaacc acgtttcaaa cttctcttag acagttccat cttttcaatt
     4201 ctttcagaag atccagacac atagttgaca cttactgttg atccttctgt tatttttgag
     4261 tcccggcgta caataacgaa tggcgcattc agataatagg acacagcttg ggcaataggt
     4321 acgccttttg tggctacagt cattacagca tcgatttttt caccaaggta tttagaagca
     4381 atgattcttc caacttgacg cagcagttca ggttctccta acaaatcaga gagataaaca
     4441 tatcctcctg gaagcagacg gtcttgttct gatagacgtt ctgctagaga ttcaatatat
     4501 tttttcgcct cttcatatga aatttcaggg atgaacaata caccacctgc agcacccggg
     4561 attgtttcca aggttccata ccctcgttct ttaaatgttt tcttgatgat tcccaaatct
     4621 tcactgatag aagatttagc agattcataa cgttcggcaa agcttgttaa ggaaatcaat
     4681 tcatgcgggt gattcaataa atactgtgtc atgtcaacta gacgttcact gcgacgtact
     4741 ttcacctatt ccaactcctt ttaagaaatt ttatcgtttt acattatata agtttttctt
     4801 caaaaattcg agcattttat gaaaaaataa gaaaatagtt cggtatttat taaaataaaa
     4861 agaagcctat aaaaaatttt tcgtttttat aaggcttctt ttctttataa tttagttatt
     4921 ttttcttctg aaggcaacca atattcgttt gatgatattt acaatcagga acaaagcaat
     4981 aaagactaat gtgattgttg ctcctggagg tgtatctaga taaaacgagc tagttagtcc
     5041 tgacagcatc cctagaaatc ctacacaaac actgatagca atcacgacat tgaagctttt
     5101 gcctaatcgc ataccgattg ctgcgggaag aaccatgatt gcggatatca aaagtgcacc
     5161 ggcaatcggg atcatcacag caatagccac accagtaatc acattgaaca tcatcgacat
     5221 caaatgaacg ggtagcccat ccacgtgggc ggtatcttca tcaaaagtca gaacatacat
     5281 cggccgtttg aataaaaaga acaatatacc gatggcaacg aataagattc ctaaaaagat
     5341 gacttgctcc catgtaatgg tcacgatcga accaaacaga taggattgga tacttgttgc
     5401 tgaattccct ccgctcaaat tcatcaatac taacgctagc gccaaaccac cagccattaa
     5461 aatggcaatg gagatttctg aataggttcg gtagatcgtt cgcagatact ccaatatcac
     5521 agctgctaat acaacgacta gcatggtcgt aagtgtagga ttaaggttca aaaagaatcc
     5581 taacgctacc cctgctaaag agacatgaga aagcgtatca gccatcagcg attgtcttct
     5641 gatcactaaa aacacaccta gcatcggggc gattccggca atgaagatag cggcaagaaa
     5701 cgctcttctc atgaattcat atgaaagcaa cgccattgag aatcctcctt tctcaccagt
     5761 cggatctgtc tgtccgcata ctgtttgata tcttcatgat catgggtgat cattagaact
     5821 gatttcccat ggacatgcgc actatgtcgg agaagacgat aaaattcatt tctggattct
     5881 tcatccatac ctgttgttgg ttcatcaaga acaaataagt cagggtcagt agcaaaaata
     5941 cgcgctaaac tgattcgctg cttttgtccg ccagacaatt cacctattcg ccgatttcgc
     6001 atatcccaca tccctacagc ttctaaagca cgctgtacat gttgatgatc tttttcagag
     6061 agacgcttga accagcggcc tcttggatat cttcctgaac ggactaattc gatcaccgta
     6121 ctaggaaaac ctgcattgaa agatgcaact tgttgaggaa tataaccgat acttaatttt
     6181 tttccttctt gatttttctc agcaatcttt atcattcctt ttgaaggttt cagtaatcct
     6241 aacgatgctt tcaccaaggt tgacttggct gctccatttt ctccagttaa aatgacaaat
     6301 tcacccgcat ctacatgata agagacatct tctagtacag gttcatcatc atagtaaaag
     6361 gtcaaatcag atatttcgat ataacgcacc ttacaacctc cattctaaaa cttaattgat
     6421 actttctttt aaagctttca gatttttttc catgacagaa atataatttt cgcctgcttt
     6481 gatttgttca ttagtcaagc tttctaaagg attcaacact tctagcttca ctcctgtttc
     6541 attcgacaat gtctcagcta ccttcgaaga ggcattttct tcaaaataaa tcactttgat
     6601 gttgtttttc ttcacaaatt cttttagctc agctaagcga cttggtgtgg gttcctgatc
     6661 tggtgaaacg cccgaaatcg ctacttgttt taagtcgtat tgattcgcta aataagcaaa
     6721 agcagcatgc tgagtgacaa atgtgcggtt tgttgcatct tttagctcat cggagtattt
     6781 ttgatccagt gcttcaagct ttttgatata agcatcggca ttcttcgtaa aagtttcctt
     6841 tttatctgga tatttttcgc ctaattcttc agcaatcgtt ctgacttctt ttatagctaa
     6901 taccggatcg agccaaacat gtggatcgta ttcatgtgaa tgaccatgcc cttcttctga
     6961 ttcttcatgt tcgtctgctt cttgcgattc taaacggtcg attcctttag ctgcttcgat
     7021 catcaaggtc tgtttgctat ccactgaatc ctttaacgaa tccacaaaaa attccatgtc
     7081 tgaactatta tacacgaaaa catctgcatc ggaaatttct gctctttctt ttgcacttgg
     7141 ttcgaaatca tgcggctccg ttccagcagg gatcaaaagt ttcacgtttc cttcatcccc
     7201 tacgatctct tttgtaaaat catacatcgg ataaaaagtc gtaacgatcg tcaaatcctc
     7261 ttttttgtct gcttccttgt tcgtatttcc acatgctgca aacagtaaag cacctatcat
     7321 taccgtagct gtcaaaagat attttttcat ttatatgctc ctttcaatga acaaatcgga
     7381 attttacgat ttattaacgt gaactaatgt atcaaaataa aaaaacacag tcaatagaaa
     7441 tccatcaatt tctgatttaa gcatgtatat tcttgattaa atcgtaaaat ggatgagttg
     7501 ctgctcatat ctagccattt cactacaata aaaaactgaa gaataccatt ttaggattcc
     7561 tcagtttttc atcacctatt ttacttctta tattttattg cagggttcgt actaaataaa
     7621 cttcctcaca aaaacctttt aacccattat atactctctg cgctcttgaa taatgttgac
     7681 acaaggcaaa aacggttggt ccgcttccac tcatcaatgc agcatctgct ccgtacttga
     7741 tcatacgctc tttgatttga tggatgactg gatgccgctt tcccgttata tcttctaaac
     7801 tgtttcccat ttttttgatc atctgctgat aatcttgctc tcggatggct tcagcaagtc
     7861 cgtggatgtc ttgatgttgg atggcttgcg tatctacttc tctaaacact gtacgtgtag
     7921 agacactcat tcttggtttg accaacacaa cccagcactg tggcatagaa ggtaatactt
     7981 ggatttcttc ccctttgcca ccgacaaatg ccgtacttcc gtagacacag taaggaacat
     8041 ctgtaccgac ttccaaaccg atttctgcca gttcttctaa cgaacagccc agattccata
     8101 attggttgat ccctcttaga gcagctgcac agtctgtact tcctccgcct aatccagctg
     8161 ctactggaat attcttctta acaaagatct tgacaccggt tttgatattg tatttatttt
     8221 ttagcaataa agcagcttga taaacattgt tccgttcatc taccggtaaa aatgctttat
     8281 ttgtttctac aatgattttg tcttctgtta tttcttcaaa aaatagatga tctgccaagt
     8341 ctacgctcga catcaccatt tccaactcat gatacccatc ttgccgtcta tataatacat
     8401 ccagtcctaa atttatcttg gctggcgctt tttcaatcaa ttccatcctc tcacccattc
     8461 gaaaagtcta agtcgcaagt attctatcat agggaaaaat aaaagtatag cttatgtctg
     8521 cctagtggtt gattttgtca tcgtttcaac agctttcaca gaattttctc ttcataatcc
     8581 aaacaagttc tagtgattct tataattctg tgagcacttc ttctgccaaa ctcggaacat
     8641 ttcctttttc ttttcctatc agactgagta gctgcatggt agcaaatccg aaattacttc
     8701 ctttcctata agcaaaataa cgtccttccc aatgatcgac atattttaat ttcttttcat
     8761 aagcagactg gaaggaatag aaaaaattac cataattata tacgatatgc atgacttttt
     8821 cttgaaagaa agaaaaatct gtttctccaa cattagctaa tggagctaag cctagattgg
     8881 cttccgagta gcccattttc tcgtatacaa taaacagatt ggctaacaga tagtcagcta
     8941 attcctcaga ttctttaggt aagacccgca acaaatcata tcccgcctgc ttcttcgtca
     9001 tcggctgagc ggtgatgaag ccaacgactt tttgatcttt acggacaata ccgacaccgc
     9061 tagataatac atatgattta tcaaatcgtc ccacagagaa gtatttttct cgtgcacctt
     9121 gaagccattt ttgtgaaact tcatttacag cttgatgtaa ttcttccggc agttgatcgt
     9181 accaagtaaa atggtatcct tctttcatca aacgttgata ttcaagttgc tgactcagcc
     9241 gagacaagtc ttctgttgct agatcaacaa tccctgcttc acctactttc gtgaagtgaa
     9301 aacccaattc atgaagcatc actacgtagc tttctgatat cttataaaac gcaagctgat
     9361 agcctaaaca atctgcttgt ttcataaact gattcgttgc tgcccgcatc attttaggat
     9421 tgccaactgg atctcctaaa acaaagcatt tgtttccttt gatctgataa ccaaaaacga
     9481 cttgatcttg gtcgtcttct tggtaataaa aataactgta acctgacaag cgaagctgat
     9541 ggcttgtagc tgtgcctccg tatgtttcca ataaacggtc aaaacggtct ttttcgtact
     9601 cactgccaag cttcgtagta tcttcagcta aatattgata aagcgccact aaagctagca
     9661 atgagatgcc caaaccaata agccctgaga accacacatc atctgaagga aacaaaataa
     9721 aatgatttcc tgttattcga ccgactaaaa agcgtccgct gtgatagcct gcaattgcgt
     9781 aaacgataaa caaaaagccg aacaagctag catcaaagat tatagctccc caagaataaa
     9841 cgaattttgt tcgataaaat tcttttctgg ataaccacac agctaacaga atcaagacat
     9901 ataccaatat caactgccaa gataacgtac gtgtgattgt atttaaaata ccaaaagata
     9961 gtaaagcaat cgtaggcaga aatgcctttt tgacttttac tgaaagtgct ctagcaaggc
    10021 cgagtaataa aaagccaacc aatagattca ctgtctgatc taaaaaattg aatgagaacg
    10081 gcaataatac ttgaaatagc cgactgattg cagacagatt agtgattgtt gatagaagta
    10141 ccatcatgat ccccgcaaaa tagacagcac ttacgagcac gatatgcgca accttttgga
    10201 aaaacagtcg cggaatgtta tctaaaaatc gattgatttt gactcctgta tgtgtgatga
    10261 acaatagaag tcccgtcata aaaggtagaa cataataaaa caaccgataa tataatagcc
    10321 aaactactgc tacatcttgc cgaacagaca attgactcat tcctaagatc atcaaaacgt
    10381 caaaagtccc cattccacct gggaccatcg tcagcattcc tatcaatgta gcaatcatga
    10441 acataggata gatcgaaagc atcgaaatat ctattctcat caacttgccg atgatcaaaa
    10501 ataccgcaag ggctcccgtc cattgtccaa aggatgccag tagcagacca agcaagcctt
    10561 ttggataaaa ctcgaagaac aatgtctttc tctttagata agcaaaaaag aaaatgactg
    10621 gtgctaaaaa actaccgccg aaaagccaga tccaatagct tcgaaaaata ctttctgact
    10681 gaataaaaaa gacatcaata aatgtaagaa acgaccatac agacaaacca gtcagcataa
    10741 atagtgcgac ttttgataca gtggctaaaa ccatttttct atttgtctgc tttccataaa
    10801 aactagcccg cagacttgct cctaccacac caccaaaacc ggcgagattg ttgatcgtgt
    10861 ttgttaccca cgcagcaaga agaaactctt ttctttccat ttttggtttt ccttgttttt
    10921 ctaagactct gatcgtcacc caatcataca acaacatcgg agtgacaccc agcagtccag
    10981 ccagaatcat gagcaggatc gtcatgcggc tttgttgtcc catcctttgc caaatatctt
    11041 gccaactcat gccattagca atatttgcga cttgatttgc cacaaagact aacacggagc
    11101 cgaaaaaaat cagttttaaa atcagactat gttcctttat ccattgcagc atcaagtatc
    11161 gtttattttt cacattttcc cattcctaac ttgtatactt ttgtgtcata ccccaatgtt
    11221 atcttattta ttttaatttt tccactttag acctgttctt tgcttgcgat gatagaaaaa
    11281 gaaagatatc ctaatgctaa aggagaggat aacatgaaag taggatttat cggcacaggg
    11341 aatatggcac aagctattat tgatggttta ttaaaaaaag gaacagtctc gaaagaagag
    11401 gtgttcgttt ccagtggaca ttttgaaaat gcacaaaaat ttgctgctga aactggcgtc
    11461 catgcatgca aaagtaatca ggaagttgca tcacaatcag atatcgttct tttagccgtg
    11521 aaaccaaaaa tcattgaaaa agtattggaa gaactaagag aatatagcca gaaagtctta
    11581 ttcatttcta ttgcttcagg aaaatcatta atagatttag aaacagcgct tgaagatcct
    11641 caggcctcca tcgtacgtgt gatgccgaat gtcaatgtca gcgtaggagc aggcgtttct
    11701 gcgatttgca aaaatgaagc tgccacggag gaacagcttc agcaagctac gatgattttt
    11761 gaagcaatcg gcagcgtcta tcctttagca gaatcagatt tcagtacatt cattggcctt
    11821 gctggcagct cacccgcatt cgtttacatg ctgatcgatg cgatgggacg gactggtgta
    11881 ctgaatggtt tgtccaaaca gacagccact gagattgctg caaaagcaat tatgggttct
    11941 tgtcaaaaat tcctttcaag tgataaaaat ccatgggaac ttgtagacca agtttcttct
    12001 cctggcggta caactgttgc aggtgtagtt gccttagaag aagcaggatt catcccagca
    12061 atcatcaaag gaatcaatac gacgatcgag aaagatcttg agatgttgaa taaaaaataa
    12121 tgatagcaat gttttttcgt gccttacaaa aaaagaagag ctgtggcttt tgccgcggct
    12181 cttcttttgt attttttatt atttagcata atcagttgca cgagtttccc gaataacgat
    12241 tactttgatg tgtccaggat aatccagttc ttcttcgatt cgtttacgaa tatcacgtac
    12301 taaacgaaca gcgtctaaat ccgagatttc ttctggcttg accattaccc gaacttcgcg
    12361 tccagcttga actgcgtaac ttgattctac accggcgaag ctgttcgaaa tattttccag
    12421 acttcttaaa cgacggatgt agttttctaa tgattcactt cgcgcaccag gtcttgctgc
    12481 tgagagcgca tctgctgcag ctacaaggac agagataaca gaagtagctt ccacatctcc
    12541 atgatgagaa gcgatcgcgt tgattactac tgggttttct ttgtacttgg cagctaattc
    12601 cgcaccgatc tcaacgtgag atccttcaat ctcatggtca agtgctttcc caatatcatg
    12661 gagcaacccg gcacgttttg ctaattggat atcttcgcca agctctgctg ctaagatacc
    12721 agctaatttt gcaacctcga ccgaatgctt caagacgttt tgtccataac tagttctgaa
    12781 tcgcagacgt ccgatgatct tgattagatc tggatgaagt gtatgagcac cgacttcaaa
    12841 cgctgcctct tccccgtatt cgcgaatacg ttcatccatt tctttacgag atttctcgac
    12901 catttcttcg atacgcgctg gatggattcg tccatcttgt atcagttttt ccaaggtcat
    12961 ccgtgcgatt tctcttcgga ttggatcgaa tccggaaaga acaactgctt ccggtgtatc
    13021 atcgatgatt aaatcgatac cagtcagcgt ctcaagcgtt cgaatattac gaccttctcg
    13081 tccaatgata cgacctttca tttcatcatt cggaagggta acgactgaaa cagtcgtttc
    13141 tgaaacttga tctgctgcgc agcgttgaat cgctaatgat aataagtttt tcgctttgcg
    13201 atctgcttct tctttggcac gctgttcaga ttcttttacc attagagtta actcatgatt
    13261 cagctcttct tcagtcgatt tcatgatgac atctcgtgcg tcctcttttg ataaaccagc
    13321 aattttttct agttcttttt gttgatcttc aatcattttt tcaacgtctt tttctcgctc
    13381 ttcaattaat tgctgttttg aactaagtgc ttcttcttta ctttcaagtg agttctcgcg
    13441 tttttctaac gagtcatctt tacgattcag cgtttgttcg cgttgaatca aacgattctc
    13501 ttgagatttt aattctaact tactttctct cagctcactt tcaacttcag aacggtattt
    13561 ctgattttct tccttagctt ctaacaatgc ttcttttttt agagtttctg cttctttttt
    13621 tgcactatcg atgattccag cagctgatga ttgtgcacca gctatttctt tctcgtgacg
    13681 tgactttgcg accataaacc ctaaaccaag accgacaatt aaaccgatga tagcgaggag
    13741 aatgttgaat accataaaat ccacctccat actatctttt tcatttttat catctataca
    13801 gcttctatga taaattattc taaactcgtc aatatgcatc ttcgcatata tgtgtacaaa
    13861 aaacatgcac aactttattc taatgtttgt accccgtagt gtcaacttta aataaacaaa
    13921 gaaacggctt tctacttatt cattaaacga tggtttttaa aataaatgac actagtttca
    13981 tataggtaaa cctttaaatt gagaaacttt tctattaaat tattctttgt agatgaacag
    14041 atatttttat tttatgtacc cactcgtata attaaataac tcacgatttc tttattcaat
    14101 aaaaaaacgg caggtcaagg agagtaccta ctcttcagac cagccgtttt tgtttatcat
    14161 aatttttatt cttctactaa aggcaattct tcttgtcctt tttcatcttc tgggacagcg
    14221 acttcttcac caataccata agctgctcta actagagcag aaacttctgc catcatttca
    14281 ggatgattag ccatatagat tttagcgttt tcccgtcctt gaccaatccg atcttcttta
    14341 taagaatacc aagcaccgct tttatcaaca atgtcttttt ctactgccat gtctagaagt
    14401 tctccttctt gtgagatccc taaaccatac atgacatcta cttcagcgat tttgaaagga
    14461 ggtgctactt tgttttttac gactttgatc tttgtacggt taccaacaat atctgtacct
    14521 tgtttcaatt gttctgcacg acgtacttcc aaacggatcg ttgcgtagaa tttcaatgcg
    14581 cgtcctccag gagtgatttc tggattcgca aacatcacgc ctactttttc acggatttgg
    14641 ttgatgaaaa tagcgattgt ttttgttttg ttgatcgaac cagagagttt acgcaatgct
    14701 tgagacatca aacgtgcttg taacccgaca tgtgagtcac ccatttcgcc gtcgatttct
    14761 gctcgtggaa ctaaagcagc aactgaatcg actactacga tatctacagc cccacttgat
    14821 actaaagcat cagcgatctc tagaccttgt tctcctgtgt caggctgtga aagaagtagt
    14881 tcatcgatat tcacacctaa tttttgtgca tattgcggat ctaacgcatg ctcagcatca
    14941 atgaaagcgg ccgttccgcc atttttttgt acttctgcaa tagcgtgtag tgcaactgtt
    15001 gttttacctg aactttcagg tccatatact tcgatgatac gtccacgggg gtaaccgccg
    15061 acacctaaag cgacatctaa cgcaagagag ccacttggaa ttgtagagat ttgttggtcg
    15121 attttttcgc ccaatttcat aatagagcct ttaccatagc tcttttcaat ttttttcagt
    15181 gcagcatcta aggctgcttt acgatcatct gccaatacga taatcctcct tatatttaca
    15241 aatcagagca acatttcatt atccttatca tatctttttt agtgaaaaaa agcaagaaaa
    15301 aaccgaacaa atattcgcat ttatcatttt cttaaaagct gttttctcaa aagatcaaat
    15361 ccttgcataa ccgcattata tctgatagca gcacgatctc tggcaaagtg atatttttcc
    15421 gaaaccacgc cgtctttaga tgaaatagcg atccatactg ttcctgcaga ttgaccttct
    15481 aacgtgtccg gtccagccac tccggtgaaa gctacactgt aatctgttct tgctatctca
    15541 cgtgcttgaa tggccatctg ctccacacat tctttgctga ctgttccata ttctgctaat
    15601 agctccggat caatctccag aaaaccagct tttgtttgga gcgagtaggt aacgaatccg
    15661 cctggaaata cttcagatac accagcaatg tcgcctaatg cggcttggaa ggcgccggct
    15721 gtcaaacttt ccgctgctgt tacagtctgc ttgttttctt tcagtagttc cactacgact
    15781 tttgctagcg aattatcatc gccgtatcca taaaaatatt cccctactcg ttcttggatt
    15841 ttttcttcta atgctaaaag agcttgattc cctgcctgaa catcatttgt tttcactgtc
    15901 agtcgtaatg tcacttcatt tggtttggca tatggtgcga tcgttggatt gatctgtgtc
    15961 tcaatcagat cttttagctc agtgaccagt cgcgattcac caatcccata aaagcgcaaa
    16021 actttagaaa ttagtttttc ttcagaagga aaacgttcct ctagtaaagg acgtacttgt
    16081 tccacaaaca ttggtttcaa ctcattaggc ggaccgggaa gaaggatata cgcatgtgta
    16141 tctgtttgat aaaaagtccc taaagcaagc cctgtacgat ttggtagagg aaccccgcct
    16201 tcgataatct gggactgttg cagattgttt ttggtcattt ttcgatgggt tgtttcaaaa
    16261 tatgccaata attttttgta tccttccgta ttttgtatga gagacttacc gaggtgagcc
    16321 gcagttgctt cttttgtcag gtcatcttct gtcggtccta atccgccaca taagagtatg
    16381 agttcacttc ttgtttcagc tagagcaatc aactcttcta aacgctgctg attatctcct
    16441 acaactgttt gataataaac ttcgatacct agatcagcta gctgttctga caagaaagtg
    16501 gcattcgtat tcacgacttg tcctagtaaa agttctgttc cgacagctat tatttctgct
    16561 ttcattactt cccgctccct tatatagtat acgctgtatt tttatatttt tttatttaaa
    16621 aagagggcaa ggagctgtga caaaaatctt gtcacagctc cttgttccaa ataaacagtg
    16681 ttctcctgcc aaaacctcgg tcataagtca caatatacat aaaacatgaa aagctgtttt
    16741 ttgaaattat cccttgtgac tcagagttag acggcaggct cacaacctct ccactaaacg
    16801 atacttacat tgatcctttg aacacaccgc tgtttttgat gaagtaatcc acacctgaat
    16861 agatagtaaa gatcagacaa gcataaagca tgatttgatc tactggaata ccgatcaggc
    16921 tgaatgggat attattgata aacagcaaga taatagcaac catttgtgta gccgtcttga
    16981 cttttcccgg ccaagctgca gccatgactt ctccatgttc gacaagtagt aaacgcaagc
    17041 ctgtgacggc taattcacga cagacaatga tcgctactac ccaaccaggg gcctttcctt
    17101 ggtcaaccaa catgataaaa gcagtcataa ctagcatttt atctgccaac gggtccgcaa
    17161 acttgccaaa attagtgaca aggcctcgcg cacgggcaat ttttccatct aaccagtctg
    17221 tgatactagc taaagcaaaa ataactgcac ctactagctg tgtgacagcc agtgtggtgt
    17281 ctcctacgtt aaattgtccc caatctaaag gtaaacctac aaccaaaatg aaaatcggga
    17341 tcatacaaat tctaagtaca gttaatttat ttggtaaatt cacactatcg actcctttct
    17401 tccctattct acgtgactat cagcgaagtt tccaccgata ttatttactt ttccaaatga
    17461 atttattcaa aggagtattt ttattgaaat tgatcttttt gaattaacga agaaggaccg
    17521 cgacacagat ttgtcacgat ccttcttttt tatacctaca agtaaacaga tgcgtaaact
    17581 aatttatacg atcattctgc gtattgaaga gtaaggttca cattttttgg actctgattg
    17641 ttttcccctg gattaactgg tacggattct ccattcgctt tgatcgttgc attgcttgct
    17701 gcaccaatcg tgatggtcgc atttgttgct gtttccggta acacggtact ctgtgtttca
    17761 tttgctgcca gtgtcccttg ataaacatag gcattgtcta ctagcacacc taaccataca
    17821 cgattgacag cagtaaattc aagggttaca ggcttggtag cgtctgtgat atttactgcg
    17881 atggcactgc cagaatcttc tcctgtagct attgccattt tcttttcttc acttgatttt
    17941 gttgttgatt ctactgtaga agactctttg gtctgcgtgc tttgctctga ggaagaggac
    18001 tcctcgactg atccttctac tttaagcgaa gaagctgtgt ctccgattat tggatcagct
    18061 tgacgatctt gccaagtcat atagcccacg acaacgacaa tcgccaaagc aaccaatcct
    18121 aataaaatga caggaagact tgtccaaaag cgacttggat gcgattcttc ctgatgcatt
    18181 gcttttctag aagtacttac tgtttcaggc tgcggacgtt cagggaccga tcctgtcaat
    18241 ttgcttttcc catcaaaaac atcgactaaa tggtctccat cttctcctac agcttcagcg
    18301 tattgccgaa taaatgcccg cacgtaaaat ttaccaggca attggtcaaa atcatcattg
    18361 tcgattgctt ccaaataacg tttttgaatt tttgtaatct gctgtaattc atctaaagat
    18421 atttttttat tcaggcgtgc ttgccgtaat ttttctccga tggtttcaaa agccactcgt
    18481 tcatcttctc cagtctgttt gattctccga agcatctgct gttgcaacga aagacgttat
    18541 gtttgggtgc tgtgacaaaa gtcatgtcac agctcctttt ttccgaataa acattgttct
    18601 cctgccaact cctcggtcac aagtcacaat atccaataaa tataagaagc tgttttttga
    18661 acttgttcct tgtgactcgg agttaaacgg caggctcacg acctctttct taaacggtgt
    18721 tcaatcagct gttcctcggt attagctcaa aaaccggcaa aatataagga gatatttttc
    18781 cgtatgtttc tctaatactc aggtcaaaac gctgatttca caacctcttc cttaaacggt
    18841 gttcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacatt aagaagctgt
    18901 ttttacttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttggtaca
    18961 cggtgttcca gaagtagctt cttcggcaat aagccgaaat atcacaaaaa tttgagaagc
    19021 aattttcgta aattccttct tatttcttga agctgaccac ttctgtcaca acctctttga
    19081 ttacttcgtt tgattttatt ttaatataag ttgattaaaa gagagcatga tttgttttat
    19141 tttctcacta atttttccaa cacaactaag aataccataa aagctgattt tggaggaggt
    19201 tcctactaca agtctaaaac gaaatttaag acttttcctc ataaaatact ggtttatttt
    19261 ggtctcatgt aaaactcgct gaaggcctct tcacttataa atgcttcccc agctgccagt
    19321 acgtctttca tttggatcga atcgatgatt tcaggaagat caaataaagt tcgatcgcca
    19381 aataaacttt gcgtaaattg attggcaata tattcgatcg aatttaatga ttggaaatat
    19441 tgtccaagca ttttcttctt cagcaaatcg agatttgttt ctgacacttc cggatcatca
    19501 gcgaatccaa gaatgatttc tttgactttt tctgctgctt tctctggttc gtccgtatct
    19561 ccgctgaagt ccgcaaagtg aaattcacga tccaagctga attcaaaacc aaagctgtca
    19621 tcgataattc cttgattgta catcgctaga tagtttcgag aagtattgcc taaaatcatt
    19681 tggaacaaca gattgattgc tgttttatag cgtaacagtt ctgttccttc ttgcggcaat
    19741 gtgtctaatc ctttaatacc aagtacaaat ttatctcgtg taatggctgc ttccaatgca
    19801 ctttgtttga tgatttcttt tgtgttttca gggaaataac gtacaatttc ttgctttgga
    19861 gggaaatttt ttgcttcttg gttttcccga ataagtttca tcaatttctc aggctccatc
    19921 ttaccaacta caaataaaac catattgctt ggctgataaa acgtccgata gcacgtatac
    19981 aagtcttgag ctgtgatttt gtcgatactt tcaaccgtgc ccgcaatatc gatatgcaat
    20041 gggtgtgtag gatacaagtt atttagtatg ccaaaaaaca tccgccagtt agggtcgtct
    20101 tcatacattt ggatttcctg accgatgatt cctttttcct tgtttacagt ttcttctgtg
    20161 aagtaaggtg cttgtacaaa atcgatcaga gtcgtcagat tcttttctac ctgatccgtc
    20221 gtagaaaaca gatagctggt tttcgtaaaa cttgtgaaag cattggcgga agctccttgt
    20281 ttcccaaata attgaaagac atcaccatct tctttttcaa acaacttatg ttctaaaaaa
    20341 tgggcaattc catctggaac tttcaccttt tctttttcac catagggaat aaattcgtta
    20401 tcgatcgaac cataattcgt actgaacaaa ccatatgtct tgtgatagtc atttttaggc
    20461 aacaaataca cggtcaaacc gtttggcagt acttcatgat aaagtgtttc attgatttgc
    20521 tcatattctg ttttattcat ttgcatttcc tcctgctaaa aagaagattg cctgcaattc
    20581 gatttgctca gctacttgtt gtatctcttt gatcgttacg ctctccatac gtttcaacca
    20641 ttcttcatca gataattttg tttccggcag ccagctgtcc aaaaaagaag cttcgattgc
    20701 cgcttgcgga gagtctagag aaagcagata ttggtttctc agcattgctt ttgtctgaga
    20761 cagttccaac tctgttattt caccatttcg caaactttct aattgctcgt gaatcagatg
    20821 aagtacttga tttcggttct tttcatcgat accggtttgg acactcatga atcctcggaa
    20881 cgtatccacg ctactagagg cataataagc tagactttct ttctctctga cattcatgaa
    20941 aagtttagag tgaggaaaac cgccaaataa accgttaaag accattagcg caaaacgttc
    21001 tggttcatcg taatacacat ttgtctgata agctaggttc agctttgctt gaacgattgg
    21061 ctcacgtact tgtcgttcct tcacgatatt cacttgaggt tgggtataaa acagatctgg
    21121 ataaactgtt tcccgcgact caaatggtaa ttgcccaact gcttcagctg ctttgtccgg
    21181 atcaacatcg ccgacaacaa aaatgtcgat ttgatcattt gccatcattt cttgatagta
    21241 ggacgctagc gaactagcag tgatcttgtc caaatcttcg actacgccca accctgggat
    21301 cttttgttcc gggctgtttt caaaataaag ttcttgcaag gcataggaag caaaagtttg
    21361 tttatcttcc ttcaaacttt ccaaatatag acgcagattg ttcttttcca agtcaaacgt
    21421 caactgatca aactgatgat ttttaatatt tggataaaat aaaacctctt ttaaaaaatc
    21481 taccgcttga gaaaaaaggt ttggatcatt gacatattca ccattcacaa aactgatccc
    21541 tgcgtttagc caatgtaaat ttcctttttt tctaactgat aatccaaagc ttgcgccata
    21601 aagctctgca agttgcgcac ttaactttgt ctgatccgaa taattcaaac tatttgtttc
    21661 caacatgctg ctgagaagtg aacgtttcgt gatgacttct tgttgcagac gcgcagtaaa
    21721 acggataaaa atccggatcg ttttgtattg ttttgttggt aagacatgaa gattcacgcc
    21781 ttctgccaat tgatacacca tatttatatg ctccttttca cagttgattg cccgcttact
    21841 cttttaatgg gtcaacctgc cttttttact tattcctctg cttgaaattt ataagttcag
    21901 gcaattcgcc gttttttcta aatgatgtta cactttatag tatagcatag atgtttttta
    21961 tcaaaagaaa cagacggcta aaagcgtata ttgttttttt attttttatt tttcgatata
    22021 ctaaagacaa ataaaagaag ggagggtcat tcatgatcaa agaattcaaa gagttcatca
    22081 tgcgtggaga tgtcttggat cttgctgtcg gcgttgttat tggtagtgcg tttacaagca
    22141 tcgtaaacca agtggtgcaa ggcttgatca caccactaat aggttggttt gtcgcaatga
    22201 tcacaggtac ttctgattta gaaggagcac tttcaattct tgactggtcg ccgacaaaag
    22261 gcgtcacatt tgcgtttggt aacgttatta gtgctatcat tacatttctg atcactggtt
    22321 ttgtcttatt cttagttgtc aaagcggcaa accgtgcgaa gaatttacgc tccaaagaag
    22381 agcaagtgga agtggaagaa actcctactg cagaagatta tcttagtgac atacgcgatc
    22441 ttcttgccaa acaagtggca gaagacaata cacatccagt agagcaaaca aatctttccg
    22501 atacttctaa ataataaaga atctaaaaaa aagaaagccg attttcagtg tcggctttct
    22561 ttttttagat tcttaacttg ctacttgttc ctttttcatg tcgatatgtg gaatatcatc
    22621 ttccaaatac acatctgaga ttgccttgaa tccaaacgac tcataaaaag cttgtaggta
    22681 ttcttgtgcg ccgatcacga ttggtttttc tggaaaattt ttctggatca tgttaatcgt
    22741 ttcttgaaca atctttcttc ctaaaccatc tttacggttg tcctgatgaa caaggacacg
    22801 tccaaaatgg atactctccg cttcttcata tactcgcgtg taggcaagga tcttcccatc
    22861 agtatcttga aaaaacgtat gccaagcttc tccatcaatg tcatcaactt cttgataagg
    22921 acactgttgt tccacaacaa aaactgcgat tcgttctttg acgatcgcgt aaaactctgc
    22981 tgttgacaat tcttcaaatt tctttacctg atattccact aagcaaacgc cccctgcttt
    23041 tttattctta gcataccgat tcgcacacaa aaaagcaaac gaaccttacg aatctcaggc
    23101 tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg agtaagtaca
    23161 tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa atcaacaacg
    23221 aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg ctttcttcct
    23281 gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt cttgattgaa
    23341 gctaaagttg ttggcataca gttcacctct actatcaact ataccacata tttttaaaat
    23401 tttcaagttt accataaaaa aaaaagaaat gcaataaaaa taaataagaa gctcattaag
    23461 atttttctaa attattcctc attataaaat ttcaagtgtt tttttatttt gagttttact
    23521 ctcccgtaag cgctcattac ttgcttgtga tccattgcta aacattcagc catgtctaca
    23581 aaatcaacgc cttgcatata ataatgaaaa attttagctt ctatttttga aaactggatt
    23641 tcgcaatttt cgattgcttc ttttaataaa agatgctgaa ttgctaaagg ttcttgcaat
    23701 gtgatctctg ctaaaaagtc tggtccttct gtctctattt tttggtcgat tgagacttca
    23761 tctttttgat cacgacgctt cagcgcgttc tgcttcctca acagagaata gatttgattg
    23821 cttataacgc ttttataata acttccaaag ctaacacctt tgcttttttc gtaagttctc
    23881 aatgcctgat ataaagcgat ccttgcttct tgttcccaat cctcagaaac ataatttctt
    23941 aaataatagg tgtttttcaa acgtagtaaa agtggaaaat atttttgata cagggcagca
    24001 aatgcttctt cgtcaccatt acgtgcagcg cgcaataact cctcagacat aaaataattc
    24061 ctccataaat tcgtttatgg aggaatctta tcatcaatga ttcatcgaac tgattagaac
    24121 gatatgttat ttattttttc tcattaaatc gtccaatttt tgggacaatt tttttaactg
    24181 ctcttcattc catggagaat tacgtcggaa atcaataaaa tgcatatctt tattttcttg
    24241 ggctattgtt ttttccgttt tttttatcgt cttatataat tcataagcag aagttcgtaa
    24301 tgctccttgg gaaaagatca tccactgttc tgctaaatcg cttgtcgcca ctgtcacttg
    24361 tgtcaaacgg tcattcagct gaccagcgat tctctcgata taactgtcag ccgtttcgtc
    24421 ttctttcgta aaaacaacat ccaactggta tttcttataa ttttgttgga tgcccggaac
    24481 tagttgggca tcgaagacaa cgatgacttc cattccttca tatttcgcat aatttgataa
    24541 acgatgtaaa agagcttctc ttgcatccgc cattttgtcc tgctttttaa gctggacaag
    24601 ttccggccac gaaccaatca tgttgtagcc gtctacaatc aacagctgtt ttttcatggt
    24661 ctactccccc tgacgttggg aaaaagcctg atacataagc aaaccagcag caacactggc
    24721 atttaagctc tggacgtgcc cggtcatcgg gatcgtcagc aattcatcca tttctttttt
    24781 caatcctgga ctcattcctt tgccttcatt accaatgact aaagcaattg atcctttggc
    24841 attccatttt tggaagtttg tgccggtcat atctgtgcca aatacccaaa atcctgtttc
    24901 tttgagctgt tgcacagctt gtaccaaatt cgtcacgcgt gcaacaggga catgttcgac
    24961 tgccccagtg gaagtctttg ttacgatagg tgtgattcca actgctcggt gttttggtat
    25021 gatcacacca tcgacgccac ttgcgtctgc tgtccgtaaa atcgacccaa aattgtgtgg
    25081 atcttcgatg ctatccaaaa tcaaaaagaa ggggtcttct tttttctctt ttgttcgttc
    25141 aatcagttgg tcaagggata aatattcata aggtgtaatc gcaagaacga ctccttgatg
    25201 cacgccgtga tcgctcaaag tttctaattt ttgtttagga acccatttga ctggcacagc
    25261 ttgttcacgt gcgatttctt tcagttcttc tactttttct cctcgtgaat cttcttgaag
    25321 aaagagttta ttccctcgcc cttgttgtag cgcttctact gcggcatggt gcccaaagac
    25381 gaaattatct tctaatgctt cttcattttg atcagaaact gactgatttc gtccagtttg
    25441 ctttttattt gaatatctat tttgtttttt gtcttcaaat ccacgtttcg attgatgttt
    25501 gtttgttctt tctttaccca tttttttcac caactttctg aatacaccag cggatcaatt
    25561 cttccatccg ctcggtttgt tttgtcaaat gcagatagcc catgagcgct tcgaaccctg
    25621 ttgcgacccg atacgtcgta acatcagcat tttttgcgct cgtatggctt ttagcatttc
    25681 ttcctctgcg ataataaagt tcttcttctt ctgtcaaaag atgttcttcc agcatttgct
    25741 gaatcaaaaa ggcttgtgca ttcgcagaaa cataattcgt tgccgcacga tgcagcttat
    25801 tgggttttgt ttctccttga tcaatcaggt ggtcacgaac atagatttcg taaatcgcat
    25861 ctccaacata agccaaggct aaaccgttca attgtgtgta atctctcatg cttctcttct
    25921 ccatcgggtt ccttgcggtg tatcttctaa taagatccct ttttctttca acaaatcacg
    25981 aatttcgtcg cttcggacaa aatcacgatt gcttcttgct tgatttcgtt cttcgatcaa
    26041 ttgttcaatc tcttcatcta ataactcttc tgctgtaaat aaaataccga agatcgaaag
    26101 ccattgcgag aataaagctt ctccttgtgc caagacagcg gcagaaactt tcggctggtc
    26161 tagataagtg ttcagccact tagcaagctc atagaccact gtgatcccat ttgctgcatt
    26221 aaaatcatcg tccatttctt cgatgaatcg ctgttctaaa ttttctaaca aagcgatttt
    26281 atccgcatca tctgccaaat tctctgaggc agtttcttgg cggaatttca aattgtcaaa
    26341 cgtattcttc agtcgttgta aattggtggc tgcttctttc atcgtcgttt cactgtagcg
    26401 gatcggacga cggtattgtg tggtcgccat gaagaaacgc aagacttgtg gatcaacgtt
    26461 tttgatcatt tcatgtacag tgatgaaatt ccctagagat ttactcattt tttcatcgtc
    26521 ttcgccaatc gttacatagc cattgtgcat ccagtaatta gcgaattttt tccctgtttt
    26581 cgcttcactt tgtgcgattt cattttcatg atgaggaaat tctaaatctt gcccgccacc
    26641 atggatatcg atcgtttctc ctaaatgttt tgttgccata acggaacatt caatatgcca
    26701 accgggacgt cctttgcccc atggtgaatc ccaagaaatc tcgccaggtt ttgcttgttt
    26761 ccataaagcg aaatctaatg ggtcttcttt ttgctgctgt tcttcccctg tacgctgact
    26821 tgctccgact tccagttcat cgatggattg gtcacttaat ttcccataat tttcaaattt
    26881 acgggtacgg taatacacat cacctgaaga ttcataagca tagccttttt caaccaacac
    26941 ttgaataaat gctaaaatat ctggcatatg atccatgact cttggatgca gtgtagcagg
    27001 ttcgacattc aacgctttcg tatcttcttc aaatgctcgg atgaaacgat cagcaacttc
    27061 tggtgctgta atacctaatt cgtttgctgt acggatgatt ttatcgtcta catccgtgaa
    27121 attcgatacg aaatcgactt tatagcctcg atattcaaaa tagcgtcgaa ttgtatcgaa
    27181 agcaatggtg ctacgagcat ttccgatatg gatataatta tacacagtcg gtccgcagac
    27241 atacataccg acatgtccct tttgtatagg gatgaattct tctttttttc tggtcaatgt
    27301 attgtagatt tttatcattc tgatctcctc actttttctc ctttgattcg aacgactttt
    27361 gctggtactc ctaccgctgt agcgtcgtct ggaacatctt tcaacacaac ggcacctgca
    27421 ccgattttgg cgttttttcc gatcgttacc ggtcctaata tctgcgcatt cgcagagagc
    27481 atggctcctt gtttaacagt tgggtgtctt ttacccgttt cttttcctgt gccgcctaat
    27541 gttacgccat ggaataaaat cacatcgtcc tcgatttctg cggtttcgcc aatgacgatt
    27601 cccattccat ggtcgatgaa cacgccagtt ccaatcgttg ctcctggatg gatctcaaca
    27661 ccagttagga aacgccagaa ttgagcattg atcttcgcca ataaaaagag ccggtgctga
    27721 tataaaaaat gagagaaacg atgccaaaac aaggcatgaa aaccagggta tgtcaaaacg
    27781 atctcagctg tcgaacgtgc agccggatcg tttctctttg ctgcatcaat cgctcgggta
    27841 aaccaactca tgactttcca ctcctttcgt aagaatttta tgtaggaaat catcttctta
    27901 taaagaaaag aagcgccttt taaataagtt tccttatcaa aagacgcttt ccgcgtggtt
    27961 ccacttttct tcataatcaa tgattatctc tgacagctaa cgcctgttat acgttcttgg
    28021 ctactttgtt tcctataaaa cagtttttcc tgttttctgt gtttcgccaa gaccactccc
    28081 agaggcattt cggcaaccat tcattgatac cttgcaccac ccggtatctc tcttgtaatg
    28141 atcagctgct tacttttttc tgttcatcgt gtttagttca gtacttgatt taaatgtgct
    28201 aatgattttt cacgaccaag caattcgatc gtttcaggca attctggacc atgcatttgt
    28261 cctgatacag ctacacggat tggcatgaaa agattttttc cttttacgcc agtttctttt
    28321 tgaacagctt tgatggcagc tttgacagtt ggagcatcta ctacttccat ttgttctagc
    28381 tgtgctttga atgcgtggag aacagtagga actgtttcac cagcaagtac ttcttttgct
    28441 gcttcattca atacaggatg ttcattgaaa aatagcgtag acagatcaac gatttctgca
    28501 gcatagctca tttgaggttg atacaggcta acgatcttct tcacccattc gattgtcttt
    28561 tcatctgggt tttctgctac acgtccatca gcgattagat aaggcagaca catgtcagta
    28621 aatgcatcta gatccattgc tttgatgtaa tggttgttta cccattgcag ttttttcgca
    28681 tcgaaagctg cgggtgattt acttaggcgt tttggatcga acattttgat caattcttgg
    28741 tgttcgaaga tttcgtcttc gcccacaggt gaccagccca ataatgcaat aaagttgaac
    28801 attgcatctg gtagatagcc taattcgcga tattgttcga taaattgcaa gattgattcg
    28861 tcacgtttgc ttagtttttt acctgtttca ctgttgatga tcaatgtcat atgtccgaag
    28921 gttggtggtg tccagccaaa tgcttcatag atcatcagtt gttttggtgt attggcaata
    28981 tggtcatctc cacgtaaaac gtgagtgatt ttcatcaaat gatcatcgac cgcaacagca
    29041 aaattataag ttggcatacc atcgcgtttt tggatgacaa agtcgccacc gatattatcg
    29101 gattcgaacg aaatagcgcc tttcaccata tctgtaaatg cgtattctgt attgcgaggc
    29161 acacggaaac gtacaacaga ttctagacct tgtgcttctt tttctgcttg ttcttcaggt
    29221 gtcaagttcg cacatgttcc tgcgtaatga ggcatttctc ctcttgcgcg ttgtgcttcc
    29281 cgttctgctt ctaattcttc ttctgtgcaa tagcatttgt atgctcgatt gctagcaagc
    29341 agctgatcaa tcagcggttg atagatttct ttacgttcag attgacggta agggccgtat
    29401 tctccaggat tttcaggaga ttcatcccaa ttcattccca accaagcaag gttttccaac
    29461 tggctttttt cgccatcttc gatattacgt ttttggtcag tatcctcaat acgaataata
    29521 aagtctccat cattgtggcg ggcaaacaaa taattgaata atgcagttct tgcatttcca
    29581 atatgcaggt gcccagttgg acttggtgca tagcgtacgc gtactttcgt cattttcttc
    29641 ctcttctttc aaactagact ttgactaaca gcaaaaattc cgtcagttac ggcttctcaa
    29701 atatcattta tttaaagcca ctcagcacaa attttacctt ttctaaggta aatagtaaag
    29761 ccaactggtt gaatttcctt accagttggc tgaatttctt ttttattttg attgtttagt
    29821 ctttttctct gtctcttttt tattgtcgat tcctcgtcct gcatgtgcag gtttagcaaa
    29881 gatcatgcgt ccagcagctg tttgaagggc acttgttaca acgacttcga tacgttcatt
    29941 catgtaatgc tgtccatctt ctacgacaac catcgtgccg tcatcaaggt aggctactcc
    30001 ttgttggcgt tctgtcccgg cttttactac tagaacattc atcgtttcac cagggatgac
    30061 gactggtttt acggcatttg ccaacgcatt gatattaaga acaggtacat tttggaattc
    30121 agagacttta ttcaagttat agtcattggt tacaatgaca ccatctaaaa gcttagccaa
    30181 tttgatcaat ttgctgtcta cttcactgat atcttcaaag tctccatcgt acatttcgac
    30241 agatataccc tcttcttttt gcaaggcatt taggatatct aatccgcgac gaccacgcac
    30301 acgtttcaaa ctatcccctg agtccgcgat gtattgtagt tcgtaaagaa caaaattagg
    30361 gatcatcaag gttccttcta agaatccagt ctttgtaata tcataaatcc gaccgtcaat
    30421 gatcacgctt gtatccaaga ttttatattt atggaaattt tctcctgatt ttcgttctaa
    30481 cagttgactc tcaacttgac tagctgtttc ttgctcgatt tttttgcttc gtgaaccaaa
    30541 aactttcttc cattcttcga ttcgtgtcgt tcccattctc aatcccagat agcctaagat
    30601 gatcatcagt aaaatcggca atacactatt gacgaatgga atttccatat tgtaaagagg
    30661 ggtcgagatc acaacaccta aagtcaatcc gatgatcgca ccgatcgcac cgaagagaag
    30721 ataagtcaag ctgacctcac ttagtttttg ttctacttgt ttgataatag ctgagatatg
    30781 tttggccaac agcaaagata ataaaaagaa aataagtgcg cctaacaggc tgttggtaaa
    30841 tgtattattc agccatccgt ttgactgttg accgattgcc tgccaagctg agggtaaaaa
    30901 tgaaataccc aagcttgcgc cgacaatgat catcagcaaa gtaatgacac gtttttgcat
    30961 cgtttttcct ccgtttcgac aatcatttat tagttagcgg aacacacgct tcaacgtttc
    31021 agccaaagtt gctactccaa ctatctcgat tccttttggc gcttcccagt tacctaagtt
    31081 gtttttaggt aggtatactt ttgtgaagcc gagtttttgg acttctttga ctcgttgttc
    31141 aatatgactg acacgtctga tttctccagt cagtccaatt tcaccgatga aacattccgt
    31201 tggctgtgtt cctttttctt tatagcttga agcaatactg acagcgatag ctaaatcaat
    31261 cgctggttca tttaatttta ccccacctgc agcttttaag taagcatctt gattctgaag
    31321 aagcaaccca gctctttttt ccagcactgc catgatcaga gatacccgat tgaaatcaag
    31381 acctgttgtt gttcgtttgg catttccgaa catagtaggt gtgactaatg cttggatctc
    31441 taccaaaatt ggacgtgtcc cttccattgc cacaacgata gcagagccag ttgcaccgtc
    31501 taggcgttct tctaagaaaa tctgtgaagg attagcgact tcttccaatc catgctcatg
    31561 catttcaaaa atcccaatct cattcgttga gccaaatcgg ttttttactg ctcgcaaaat
    31621 ccgaaaacta tgatgttgtt caccttcaaa atataacacc gtatccacca tatgttctaa
    31681 cattcttggg ccggcgatcg agccttcttt ggtaacatga ccgacgataa aaatggctat
    31741 cccatttgtt ttagcggttt tgagtagttc tgcagtggtt tctcttactt ggctgacact
    31801 tcctgccaca cttgtgatgt ctggctgcgt catcgtttgg atggaatcaa tgataacata
    31861 atcaggggag atatgttcga tggcacgact gatttcgttc atatctgttt cagcgtatag
    31921 ataaaattct gtgttgatac ttccgaggcg ttccgcgcgt aattttatct gctctgcgct
    31981 ttcttcgccg gatacatata agacttttcc gcctatggct gctaattgct gagaaacttg
    32041 taacagcaaa gtggatttcc cgatacctgg atcgccacca atcaagacca tagaccctgg
    32101 caccacacct ccgcctaaca cacggttcag ttcttctaat tttgttttga tccgaggttc
    32161 ttttttaggg acgacgtctg cgatttttgt tggttttgct ttttctcctg tcaaacttgt
    32221 ccgagttctg cggtccgttg tatcttgttc gatttcttcg accatcgtat tccattttcc
    32281 gcaatttggg cagcgtccca agaacttagg cgacacatac ccgcaatttt ggcaaacaaa
    32341 ttgtactttt gatttttttg ccattgtacc gcctcctttt atcctaatca tgagacatta
    32401 aatcaagctt aagtatcttt aacgttttat ttactggaag aaccaaatcc gccttgacgt
    32461 tgagcagtcg tttgatcttg atctgctttt aaaaacggca aaaagatccc ttggccgata
    32521 cgctcgcctt ttttgatcac tacatccttg aaaccaaagt tggtgaattg gaacatgata
    32581 tggccttcat ttgcctcatt attgtaataa tcactgtcaa tcacacctac tccgtttgtc
    32641 aacactaaaa accgcttcaa cggattgctt gaacggttag caagctgcaa aaattcatct
    32701 tctcccatgt aagctttgat tccagtaggc acgagggttg gtttcaattg tttttcattt
    32761 tctgatacat cttttttcaa tccatctgcg actgtttcca accccttttt ccagcctgct
    32821 ttccagatac ttggaatcac gacctcttct gctgcttcaa aatcatagcc agcagcatga
    32881 tgtgtagcgc gctgcggtag attgatattt tgatctttat atttagaaat aacttcaaat
    32941 cctctttttc tcaccggttt tcct
//
LOCUS       DASNNU010000023        32323 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.023, whole genome shotgun sequence.
ACCESSION   DASNNU010000023 DASNNU010000000
VERSION     DASNNU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.023"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            56..802
                     /gene="truA"
                     /locus_tag="U8116_001023"
     CDS             56..802
                     /gene="truA"
                     /locus_tag="U8116_001023"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN8434032.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(1257..3926)
                     /locus_tag="U8116_001024"
     CDS             complement(1257..3926)
                     /locus_tag="U8116_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEN8434033.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(4164..4352)
                     /locus_tag="U8116_001025"
     CDS             complement(4164..4352)
                     /locus_tag="U8116_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEN8434034.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            4622..4984
                     /locus_tag="U8116_001026"
     CDS             4622..4984
                     /locus_tag="U8116_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN8434035.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5036..6718
                     /locus_tag="U8116_001027"
     CDS             5036..6718
                     /locus_tag="U8116_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEN8434036.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            6835..8871
                     /gene="recG"
                     /locus_tag="U8116_001028"
     CDS             6835..8871
                     /gene="recG"
                     /locus_tag="U8116_001028"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEN8434037.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            8920..9921
                     /gene="plsX"
                     /locus_tag="U8116_001029"
     CDS             8920..9921
                     /gene="plsX"
                     /locus_tag="U8116_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEN8434038.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            10043..10285
                     /gene="acpP"
                     /locus_tag="U8116_001030"
     CDS             10043..10285
                     /gene="acpP"
                     /locus_tag="U8116_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN8434039.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            10553..11557
                     /locus_tag="U8116_001031"
     CDS             10553..11557
                     /locus_tag="U8116_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8434040.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            11558..12502
                     /locus_tag="U8116_001032"
     CDS             11558..12502
                     /locus_tag="U8116_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8434041.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            12502..13464
                     /locus_tag="U8116_001033"
     CDS             12502..13464
                     /locus_tag="U8116_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8434042.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            13491..14405
                     /locus_tag="U8116_001034"
     CDS             13491..14405
                     /locus_tag="U8116_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8434043.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            14431..16212
                     /locus_tag="U8116_001035"
     CDS             14431..16212
                     /locus_tag="U8116_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN8434044.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            16560..17246
                     /locus_tag="U8116_001036"
     CDS             16560..17246
                     /locus_tag="U8116_001036"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEN8434045.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            17266..20847
                     /gene="smc"
                     /locus_tag="U8116_001037"
     CDS             17266..20847
                     /gene="smc"
                     /locus_tag="U8116_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEN8434046.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            20856..21665
                     /locus_tag="U8116_001038"
     CDS             20856..21665
                     /locus_tag="U8116_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN8434047.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            21676..22674
                     /gene="ftsY"
                     /locus_tag="U8116_001039"
     CDS             21676..22674
                     /gene="ftsY"
                     /locus_tag="U8116_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEN8434048.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            22861..25128
                     /gene="gshAB"
                     /locus_tag="U8116_001040"
     CDS             22861..25128
                     /gene="gshAB"
                     /locus_tag="U8116_001040"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEN8434049.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(25217..25669)
                     /locus_tag="U8116_001041"
     CDS             complement(25217..25669)
                     /locus_tag="U8116_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEN8434050.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            25819..26769
                     /locus_tag="U8116_001042"
     CDS             25819..26769
                     /locus_tag="U8116_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN8434051.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            26762..27721
                     /locus_tag="U8116_001043"
     CDS             26762..27721
                     /locus_tag="U8116_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN8434052.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            27718..28473
                     /locus_tag="U8116_001044"
     CDS             27718..28473
                     /locus_tag="U8116_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN8434053.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            28496..29449
                     /locus_tag="U8116_001045"
     CDS             28496..29449
                     /locus_tag="U8116_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN8434054.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(30093..30389)
                     /locus_tag="U8116_001046"
     CDS             complement(30093..30389)
                     /locus_tag="U8116_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEN8434055.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            30744..31100
                     /locus_tag="U8116_001047"
     CDS             30744..31100
                     /locus_tag="U8116_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN8434056.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            31110..32009
                     /locus_tag="U8116_001048"
     CDS             31110..32009
                     /locus_tag="U8116_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEN8434057.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        10708 a         5732 c         6906 g         8977 t
ORIGIN      
        1 tttatagaaa aatcattatt ccagataacc ttttaggaac aaaggagaga aaaacatggt
       61 acgttacaaa gcaatcattg cgtacgacgg aacaaatttt aatggattcc aaaaacagcc
      121 aaatgggcgt actgttcaag aagaagtaga aaaaacatta caaaaaatgg caaatggaaa
      181 agaaatcacg gtctttggtt cgggaaggac agatgccgga gtacatgcga tcgggcaagt
      241 aatccatttt gattatccag aggagcgtcc gttagaacga atgcgtttcg cgctagatac
      301 ccagtcacca gaggatattg ctgtaagaca agtagaaatt gtttcagaag acttccacgc
      361 aagatatttg gtcaaagaaa agacgtatca atttcgagtg gatataggga aaccacgcag
      421 tccttttcga cgccattatg caagttattt tccttaccca cttgatttgg aaaaaattca
      481 gcgggcgctt cctgatttat tagggactca cgatttcacc tctttttgtg catcaggaag
      541 ctcgatagag gataaaatac gtacgatcta cgaggctaag atggaggtaa atgaaacaaa
      601 agacgaattg ttgtttacat ttaggggaaa tggcttcttg tataagatga tacggatctt
      661 agtaggaacg cttttgaaaa ttggcaatgg ccgattgcct gaagatagta tcccagatat
      721 tattgcgaaa aaagatcgta atgcagcagg acctacagca caccctgaag gactttattt
      781 gtatgaagtg aaatatgaat aagaggttat gatagaagtg gtcagcttca agaaataaga
      841 aggtatttac gaaaattact tctcaaaatt ttgcaaaatt tcggcttatt ttcgaagaag
      901 ctacttctgg aacaccgttt aaggaagagg tcgtgaaatc agcgttttga cctgagtatt
      961 agagaaacaa acgggaaaat agctcctcat attttgccgt ttgttgagct aataccgaag
     1021 gtcagctgat tgaacaccgt ttaagaaaga ggtcgtgagc ctgtcattta actctgagtc
     1081 acaaagagca atttcaaaaa acagcttctc atctttgatg aatatcgtga cttgtggacg
     1141 aggcgttgat aagtgaatca tctttttgtt acctattttt tctgtaacca aaaaacgatg
     1201 gaacgttgtt ttgattgccc ttggtgcgga cagtctgaac aggttccatc gtttctttat
     1261 tttctacgtc ttcttctgtg tgtgtttcgt ggttgttctt ctattgctgg atcaatcgtg
     1321 ccataaaagg ggcgattgcg gtcaaggtac cagacataaa gaataaacaa tgcaatacat
     1381 aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca agtaactgaa agtgacagta
     1441 tgtttacctg ctggaacttt tatcatcaag aatgcgtctt tgaaattgct gaccttgact
     1501 ggtttcccat caattttagc tttccatcct ttgtcataag gaatagttgt caccaacatg
     1561 gaatcttcag cggtatttac agtcccagaa gcttttctgc cagtggcttc catttcgaca
     1621 cctttttctt tgatctgatt cattgcggct tgataggcta caacatctag accaacgact
     1681 ttaggctcca tgaaacttat ttctttcgtc ccataaaaac ttgcagtaaa tgtcactgtt
     1741 gtctcttgag gataatagcc aagatcatag tattgaccag aaatgttgat ttgcgttttt
     1801 cgcgatgtac cattgacagt gatcgtcgca ctagaacttt ctaattgcgc aaaattggtt
     1861 gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat ggacttccca agtgagctct
     1921 tttggctggt tatctgctgt ttctgagtaa gtgacgccag tcgttgtctt tgtgatcgtc
     1981 gtattcaact gattcttcaa agtaggtgta tagaatgtaa aatactgttc atttagacca
     2041 gacaatgtat tgatcaagtt tgtctgactg gtcaaattat ctgtcactgg ctgctcaaca
     2101 tcattaacgg acaacggtgc agtaaatgct agtggcaatg cattgacatt ctgatacagt
     2161 gtatagtcgc cgctttgacc agcttggcgg aagccatatt tactgaacgt gctttcgttt
     2221 ttagaaatgt tatatttgat tcctgtaaac ccatccatca gcaaagtatt attgttataa
     2281 cggatattca aatttgtccc tcttgaacgg aaacctaatt tatccaggta agaggaagaa
     2341 ttacgatttc ttatagaaga aaacaaactg ataccactgt aaccatagtt gatcgaatcg
     2401 tttgcagata ctggatccag attttccaag cggtaaaacg agtcggaatc tttttttgtt
     2461 tcatttacaa gagctttcaa gtcaggataa ggatcagtat ataaactgcg ggaagcataa
     2521 ttccaatcat caagaatgcc atggatcata ctgttcgtat tgaccagtgc ttcagcagac
     2581 atgatcaata ataacaatat actcagatga ttgaatgaaa gttttttatg ctgataaaat
     2641 cccacagcaa aagcataaat ccctaagaaa accactgtca atacaaagga agtcatcgtc
     2701 acataggtgt agcttccttc aggtttgatg ccccatgcca ccgcaaagag agcagctacg
     2761 accaaaatga ttcctaacaa cagcccacga tcgtcttgtt ctatcttttc ccatcctttt
     2821 cctgcaagca ggagcactaa gaaagagaac agataggcat aacggaataa aaacatattc
     2881 ggtgcatgca taccatgcca gaacaaattc aatggaacaa gataaaaact tgcgataagt
     2941 atggcgaaaa gactgccgta caaaaattta tttttcaacg gtactttccg actggcaaaa
     3001 taaaacagac agaggactaa tggtagcaga cctgcgtaaa cgaacggaat cgatccgtat
     3061 tttgtcgtgt cataaacacc aatcatattt ttcatgatta gatccagata agaagtcgct
     3121 tctgttttca acgtagtgat ctgactcagt gcctcgccat ttgaccgaag atccagcact
     3181 gctggcaata caatgatcat tgaagcgccg cctgccagta aagaagtgat accataagga
     3241 acaatcgttt ttttatattc tgaccagttt gtcaatagac gggcgataaa atataagaaa
     3301 gaaaatacgc caatcataaa acccatataa aaactggata aaaacaagag taaataactg
     3361 ataaataaaa gtttcggccg tttaagatcc ataacacgat gaatccccaa aatcactaat
     3421 ggaagataaa aaaatgcatc cagccacatg atgatctccg aatgtgcagt gataaatgag
     3481 atcaatgcgt aactgacgct aagtgcaaca tgcccccagc gaggaatctt gaacgtacgt
     3541 gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa gcaaagtcag aacatacagt
     3601 gcatctggca tcatctggtt tggaaaaaag aacaccagtg gcgtgaagat cccgcctaga
     3661 tagtaagaaa ttaaagataa ataattcaat cctagagaag cattccatgt ataaaaaata
     3721 ctttgctttc caagaagaac atttcggaag ctggcatgaa aattagaaaa ctgtgaaaag
     3781 gcatcacttg ccaaaatact tctgctgctt ccaggataga ttccgatact tagataaaca
     3841 agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg cgtagcgtcc gttttctcta
     3901 aagaatgact ttaaattttt tttcatattt attttccacc aaatttctat gaagttgtat
     3961 tcagtttatc atatccttgt ttttctctgc ttaaaaagag tctagttttc acaaaaaaat
     4021 aaaatttaga ataaataact tcataatttg ttcgtataac aaaaataaac gcaaaaaaag
     4081 ggtaggaatc aaaagtccca cccttaaaaa tttttattaa ataaaaaacc gttcactatc
     4141 tgaacggtcc aagtatcatt caattaaacg cgttcaactt ttcctgattt caaagcacga
     4201 gttgacaccc aaactttttt aggttttccg tcgaccataa cgcgaacttt ttgtaagtta
     4261 ggtttaacag tacgttttgt tgagttcata gcgtgtgaac ggttgtttcc actttttgtc
     4321 ttgcgacctg taaagtaaca aacttttgcc atggtaattt cctcctttgc cttgatttgg
     4381 agcaatagaa taattcagct cataccagac taacatatca tatagaatgg gaatttgcaa
     4441 gtacattttc atagatacaa ttgacaattg agaagaataa ttgtaaatta aacaaggtta
     4501 tacctgaaat tttgcaaggt ttttgagtac tttgggtaac tgccttttat ttcaagttgc
     4561 gctatgataa aatatgatag aagaaaaatg ggagtttttt acccgttaag gaggctcatt
     4621 catggctgta aaaattcaaa cgtcagctgg aaccattgaa attaccaatg atgtaattgc
     4681 aacagttgtt ggtggcgccg cgacagatgt gttcggtgtt gtaggaatgg ccagcaaaaa
     4741 tcaaattaaa gataatatta ctgagatttt gcgtaaagaa aattactcaa gaggagtagt
     4801 tgtccgccaa gaagataatg gtattgctgt agatgtttat acgattatca gctatggtac
     4861 gaaaatctct gaagtttccc gcaacgtcca agaaaaagtg aaatacaatc tagaggcatt
     4921 attaggcgta actgccaatt cagttaatgt ctttgtacaa ggtgttcgtg tactgccaga
     4981 ctaggcagga gactggctgg taactcagca gtcgatctga aggaggatta tctaggtgag
     5041 tgtaacagaa ataagtgcaa gccagttcca ggaaatggtc caggcaggtg cgaatcgtct
     5101 gcaagtaaat gcagaatacg tcaattcatt aaatgtattc cctgtgccag atggcgatac
     5161 gggaacaaat atgaatctat ctatgactag tggagctaag gcagtagtag attcaacctc
     5221 agaaaaagta ggagaattag cagcaatgct ttcaaaagga ttgctgatgg gtgcgcgcgg
     5281 aaattccggc gttattttgt ctcaattgtt tcgtggattt tctaagcaaa ttccagatgt
     5341 tgtctcatta aatgcttctg atcttgctgc agccttcact catggagttg aaactgcgta
     5401 taaagcagta atgaaaccag tagaaggaac gattttgaca gtagctagag aagcagcgaa
     5461 agctggagaa aagaaagcaa aaagtactga tgatgtcgtt gaagtaatga cagctgttgt
     5521 aaaaggagca aagcgtgctt tagctaaaac tccagacttg cttccagtcc taaaagaagt
     5581 cggtgtcgta gacagtggag ggcaaggact gctgtttgtt tatgagggct ttttaagtgc
     5641 attgaatgga gaattccaag cggatgaaac atatgaacct tctccagcag aaatggatga
     5701 tatggtcaat gcagaacatc atcgcagtgt ccaaggtcaa atggcgacag aagatatcca
     5761 ttttggttac tgtacagaaa tcatggtaaa aatcggagaa ggacctacag tcgatagtac
     5821 attcgattac gaagaattcc gtaactattt agatggtata ggagattctt tacttgtggt
     5881 caatgatgac gagatcatca aagtccacgt gcatacagaa aacccaggag aagtcttgaa
     5941 ttatggacaa aaattcggtt cattagtcaa aatcaaagtc gacaacatgc gcttgcagca
     6001 tgagacgatt ttggaacacg atgaagaagt gaacgagttt gcgaaaaaag aaataccacg
     6061 tatcccgtat ggtatcgtag cgatcgctgc tggtaaaggc gtccaagagc tgtttgaaag
     6121 tttaggcgct aattatgtta tcagtggtgg acaaacaatg aatccaagta cggaagacat
     6181 tgttaaagcg attcaagaag taaacgcaga taaagtgatt gtcttgccaa acaataagaa
     6241 catcttcatg gcagctgatc aagcagcaga agtagcagat atcccagtag cagttgttcc
     6301 ttcaagaacg atttcccaag gattgacagc tatgctttca ttcaatgatc aagcttcttt
     6361 agaagaaaac aaagaaacga tgacagaaat gttgtctagc gttgtgagcg gtcaagtcac
     6421 tcacgcgatc agagatacct caattgacgg tgtagagatc aaagaaggag actttttggg
     6481 catgatcgat ggaaaaatcc tgatctctaa cccagatatc acagcaacag cgttaagtac
     6541 actagaacaa atgatcaatg aggacacaga gatcgtaacg atcttggcag gtgatgaagg
     6601 aacagttgaa caagcaaatg aatttgctga gaaactaaca gaaaaacatc ctgatttgga
     6661 aatcgagatc caccaaggag atcagcctgt ctatccttat ctattttctg ctgaataatt
     6721 tctgtgtcag aatatctttc taacacagta ttcattcata aaaggccttc ggttagtgat
     6781 caggtatgat cgctgaccaa gtcctttttt tgttaaagga gggaggaaaa atttatgcgt
     6841 tctttagatg atccaatcac tatgctggca ggagtcggag aaaaaagagc agctagtctt
     6901 gccagcttgg gcatccaaac gattgaagac ttactgactt attatccttt tcgctacgaa
     6961 gatattcagg aaagaaatct aaacgagatc caggatcaag aaaaggttac tttgaaagga
     7021 atcgtagtct cgcctcctgt gatgaatcga tttggttata aaaaaagccg attgcaattt
     7081 cgtatgatgc aagaacatga tgtgttcaac gtttcttttt ttaatcaacc ttatctaaaa
     7141 gaaaaagtca ttttatcgga ggaaatcgcc gtatacggta aatgggatgc aaagcgtaaa
     7201 gccttgaacg ggatgaagat tttaggttct caaacaatgg atgacttttc gccgatatat
     7261 cacgtcaaca aatcaattcg tcaaacgaca ttagttgatc taatcaaaaa agggtttcaa
     7321 gaatacggca cattgattga agaaaatctg ccaaacaacc ttgtggagaa atatcgactg
     7381 cttgaccgag cttctgctgt aagaagtatg cattttccaa aagaccacga agaaaaccat
     7441 caagccaaac gacgtgtcgt ttttgaagag ttctttttat ttcaaatgcg tcttcaagga
     7501 ttgaaaaaag cagaaaaagc agaaacgaat ggtttggaga tcctttatga tgtccaaaaa
     7561 ttgaaacaat ttactcaaaa gctacctttt gaactgacaa atgcacaaaa acgtgtaacg
     7621 aatgaaatct gtcgggatct tcgcagtccg cagcatatgc agcgactttt gcagggagat
     7681 gtaggtagcg gaaaaacagt ggttgccgcg attgcgttat acgctaccgt tacagcaggt
     7741 ttccaaggtg cgttgatggt gcctacagaa atcttggcac aacaacatat ggaaagcttg
     7801 caacagctgt ttgatcccaa tgaagtaacc atcgcattga ttacggggtc aacgaaagca
     7861 aaagaacgac gtgagctcct tgaaaaacta gagcaagggg aaattaacgt cgtgatcggc
     7921 acacatgcat tgatccaaga tggagtcgaa tttcagaatt taggtctagt tattacggat
     7981 gagcagcacc gttttggcgt gaaccagcgg aaagtacttc gtgaaaaagg ctggcgacca
     8041 gatgtcttat ttatgacagc gactccgatt cctagaacat tagctattac agcatatggt
     8101 gagatggatg tatctatcat tgatgaactt ccagctggac gtatcccgat cgaaacacgc
     8161 tgggtaagaa cacctcaatt ggacagtgta ctggattgga cgaaaaaaga actggcaaga
     8221 ggacatcaaa tgtatgtcat ctgtccgctg attgaagaat cagaagcatt agatgtaaaa
     8281 aatgcagtgg aaatctatga aaaacttcgt gaactatttg caccaaaata tgaagttggc
     8341 ttgttacatg gtaaaatgaa gaatcaggaa aaagaagtaa tcatggaagc cttcaaagac
     8401 aaccagatgc agatattagt ttcgactact gtaattgaag taggggtaaa cgtgccgaac
     8461 gcgaccgtca tgctgatcat ggatgcggat cgatttggat tggcacagct gcaccaactt
     8521 cgtggacgtg ttggacgcgg aagtgatgct tcctactgta tcttagttgc taatcctaaa
     8581 aatgaactag gtgtggaacg gatgaaaatc atgacagaga ccaccaatgg ttttgtacta
     8641 agcgaaaaag atctggaatt acgtggtcct ggtgaagtat ttggcttccg tcaatctggt
     8701 ctgcctcaat ttgcgattgc tgacttggtg acggatggaa atgttttgga ggtagccaga
     8761 gaagaggctg cagccctttg gcaaataaag gattggcaga tgcttcctga gtatcagcct
     8821 ttagcgcaaa gcctccaaat agaaaatcag gataatcgtt atttcgatta gcagactgtg
     8881 ctatacttta tactgatgac ggaaaaagga gtggaaacaa tgaaaattgc cgtagatgca
     8941 atgggcggag atcacgcacc tcaagcaatt gtagaaggcg tggctttagc gcagcaagat
     9001 tttccagaag tagaattctt actttacgga aaagaagcag aaatcaagaa atacttaaca
     9061 aatgaaaaaa acatcacgat tatccataca gacgaaaaaa tcaatagtga tgatgagcca
     9121 gtaaaagcaa ttcgacgaaa aaagactgca tcaatggtcc ttgctgcaca agcagtgaaa
     9181 aacggtgaag cagatgctgt attttcagca gggaatacag gcgcgctact agcagcaggg
     9241 ttgttcatcg taggacgtat caaaaatatc gaacgccctg gcttgatgtc taccttgcca
     9301 gtagtaggga aagaaggtgc cggtttcgat atgctggatc ttggcgctaa cgcagaaaac
     9361 aaaccagaac atttgttgca atacggtatt ttaggttctt tttatgcacg taaagtccga
     9421 ggtatcggac gtcctcgtgt cgcactctta aacaacggaa cagaagcaac aaaaggtagt
     9481 gaagtgacga aaaaagccta tgaactcctt cagaatgaaa caagcttgaa ttttattgga
     9541 aacgtagaag ctcgtgagct actcaatggt gtggctgatg tagtggtcac agatggattt
     9601 acaggcaacg ctgttttgaa atctattgaa ggaacagcaa tgaatatgat gaatctatta
     9661 aaatctgcta ttctaaatga agggatcaaa ggaaaaatgg gcgctatgct tttaaaagac
     9721 ggcttacgtt ctttgaaagc agaaatggat tattccaaac atggcggtgc cgttttgttt
     9781 ggcttgaaag caccagtcat taaaactcat ggagcaacag gccccgatgc tgttcgctat
     9841 acgatccgcc aaatccacac aatgcttgag acagatgtag ttggacaatt agtcgaacaa
     9901 tttgaaaaaa gcgaagaata gtatttggat atctgaagcg atccgttcca ttgaaaaaaa
     9961 ggtggacatt cctgaaaaat gccagtaaaa ttagccatgg tgtaggtaat tgacaagtgt
    10021 atgattcttg gaggtgaaaa agttgactcg cgaagaagta tttaacaaag tagcgaaaat
    10081 tatttcaaat cattttgaga tagataccga taaagtgacg gatgaattga acatcaaaga
    10141 tgatttaaaa gctgattcaa ttagtattat ggaattcgtt ttagagcttg aagatgaatt
    10201 cggtacggaa atttcagatg aagacgctga acaaattgaa acagtcggtg gcgcagtaga
    10261 ttatatcagc agccatctta aataatttca aataaaagca aagaaggata aaaagctgca
    10321 tgcagctttt tatccttctt tttttaatct ttttgacacg taaaagtcat ttttctgata
    10381 attctggcga taaacagaaa ttatgttgca aagtaaagcg gttttctata taattattaa
    10441 taattcatta cacaaagata agaataacct ttgttataaa acgatttatt ctgtgtaatg
    10501 tcctttttta ttaagaaaaa cttccattta ggaagcagag aggggtattc gattgtccga
    10561 taaccaatta ttagaagttc agaacctaca tacaggattc cgactcaaag atgactatta
    10621 cgatgcagta gatgacgttt catttacttt agacaaaaac gagattttag caatcgttgg
    10681 ggaatctggg tgcgggaaaa gtaccttggc tactacgatt atgggattac ttgatcctaa
    10741 taacacaaaa attacaggtg agattttata taacgatctg aatttaatcg acctgaacga
    10801 aacgctctat aataaaatcc gcggaaatga tatcggaatg attttccaag atccgttgtc
    10861 tgcattaaat cctctcatga gaatcgaaga ccagatcaaa gaaggacttt cctatcacac
    10921 aaagatgaat gcagaacagc gtcaagctcg tgcactagaa ttgctagaac aagtagggat
    10981 tcctaatcct gcgagagttg gacgccaata tccccatgaa ctatcaggcg gtatgagaca
    11041 acgtgtcatc atcgcaatcg caatcgcctg caaacctcca atcgtcattg cagatgaacc
    11101 gacgacagcc ctagatgtaa ccatccaagc acagattctt gatttactga aagaccttca
    11161 ggaagaaaca aaaacaggga tcatcttgat cactcatgac ttaggagtcg tagcagaaat
    11221 ggctgatcga gtggcagtga tgtatggcgg acagttcgtt gaagtagcag gtgtaaaaga
    11281 actgtttgat catcctaagc atccttatac acgctcttta ctgaactcta taccacaaga
    11341 aggaaatcat gatgcagaac tccatgtgat cgaaggtgtc gttccttctc tgaaaaacat
    11401 gcctcgtaaa ggttgtcggt ttgcaccacg tattccatgg ataccagcaa gcgatcatga
    11461 agaagagcct atccttcatg aagtcgcacc gaaccacttg gtacgttgct cctgctataa
    11521 gcatttccac tttagagacg aaaaaggaga ggtgtagatg acagaattaa tcacaatcaa
    11581 agacctgaaa gttcattacc ctattcgcag tggattcttc aatcgagtaa cggatcatgt
    11641 atatgctgta gatggtgtgg attttattat cgaaaaagga aagacatatg gtttagtggg
    11701 agagtctggc tctggaaaat ctactacggg aaaagccgtt gtgggattag aaaaggtaac
    11761 agccggacaa atcatgtatg aaggaaaaga tgtaacgaag cgaagcaacc gcaaaaaaat
    11821 gggttataac aaggatgtgc agatgatctt ccaagattca atgtccagtt tgaatcctaa
    11881 aaagcgagtg ttggatatca ttgctgaacc catccgaaat tttgaacggt taagcgatca
    11941 agaagaaaag aaaaaggtaa aaggactgct agatatcgtt gggatgccgg aagatgcgct
    12001 atataaatat cctcatgaat tttcaggcgg acaacgtcaa cgtctaggag tagcccgtgc
    12061 tgttgctacc aatccaaaac tgatcgtagc cgatgaacct gtatctgctc tggatctatc
    12121 tgttcaagcg caagtattga atttcatgaa acgaatccag caagaatacg gcctcagcta
    12181 tttattcatt tcacatgatt taggtgtagt aaaacacatg tgcgacaata tcgcaattat
    12241 gtacaaaggt cgatttgtag aaatcggtac aagagaagac atttataatg acccgcgaca
    12301 tatctatacg aaacgtctgc tttcagctat tccgcaaatc gatgtagaaa atagggaaag
    12361 ccacaaagaa aatcgacgaa gagtagaaca agagtatatc caaaaccaaa aagaatacta
    12421 tgatgcgact ggtcgagtct acgatttgcg tacgatcaca actacacaca aagtggcgtt
    12481 gaaagatgga ggtgcaagct aatgtggaaa acgattctgc gacgtgtttt attgatgatc
    12541 ccgcaagtta ttattttaag tgttctgatc ttcttattag cccaagcgat gccgggggat
    12601 ccatttaccg gtttgatcaa tccaaatcag gatccggcag tgatcgaaca gatgcgacaa
    12661 gcagctggct tgaatgaccc atggtatgtc cagtacttcc gttggatcgg aaatgctttg
    12721 caaggcgatt ttggtcaaag tttcttatac aaacgctctg ttgctgactt gatcggcgaa
    12781 cggatcatca acactttata tctatcgatc ttgacagttg tccttactta tttgattgct
    12841 gtgcctctgg ggatgcttgc tggccggtat caaaattcaa tcttggataa gattgtcgta
    12901 atctacaact tctttagttt tgctgttcct ttatttatct tcggcttgat catgctattt
    12961 gtttttggtt atcgtttagg ctggttccca acaagtggat cacaaacact aggtttgcaa
    13021 ggtggattta tcactcaatg gatagaccgt ttgaaatata ttctgttacc atcaattacc
    13081 caagcatttt tggcgactgc cgtcacaatc caatatctgc gaagtgaagt aatcgattcg
    13141 aaatcgctcg attttgtcag aacagcccgt tccaaagggg taccgacgaa taaagtattc
    13201 agtcgacata ttttccgaaa tgcagcgttg ccgatggctt ctcaaatggg gtatgaaatc
    13261 acttcgttga ttgctggttc tgtagttatc gaaaagattt ttggttatcc gggagtcgga
    13321 aaactattta ttgattccat cggccaacgg gactataccg tgatcacatc cttagtattg
    13381 attttaggaa tcgcgacgct agtggggaca ttactttcag atatcatcat gagtatcgtt
    13441 gacccgcgta ttcggattca ataaaaccaa tcagaaagga gaatggatcg atgagtgaaa
    13501 aaaacaatac agcagttcaa gaaagtattc ctcctatggg gatacgaatg attgctcgag
    13561 aatttaaaaa agataaaatt gctatttttt cattaggttt gctagtggtt ttattattag
    13621 tgatctttat cggtgcgatg ctgaccgatc aagacaaagt gatgtatgtt agtatcttcg
    13681 acaaatacgc tgcaccagga acagtttcga cacaaggaca aaagttcatt ctgggagcag
    13741 atgaaggcgg acgtgatgta ttaggacaat tgatcatagg tgctagaaac tctgttacga
    13801 tcggatttgc gatcacgatc cttacttcga tcatcggggt tggattaggg atcatctctg
    13861 gctactacgg agggatcatt gacaacatct tgatgcggat cgttgatttt atcatgattc
    13921 taccgatcat gctgatcatt atcgtcttcg tatcgattat ttccagatac aatgtctggt
    13981 cctttatatt catcatgagt gcattttatt gggtagctaa agcccgatta ttccgaagca
    14041 agacattatc tgaagcaaga agagattatg tcagtgcctc aaaaacaatg ggaaccaatg
    14101 attttaaaat catgttccgc gaaatcatgc cgaatctaag ttcattaatc attactaatt
    14161 tgacgatgaa ctttgcggca aacatcggta tcgagacaac tttgactttc ttaggtttcg
    14221 gcttgccggc aaatgtacct agtttaggta cattgatcgg ttatgcaagt aacggagaag
    14281 tcttagtgaa tcggcaatgg atctggttac ccgcatcaat ccttatttta gttttgatgt
    14341 tgagtatcaa ttatgttgga caagcattca agcgttctgc agatgcgcgc caacggttag
    14401 gctagtatag aagcgaaggg ggaaatagga atgaagaaaa cgatttttgg atttgcagcg
    14461 ctgatgtctg ctgtcgttct tgcagcatgc gggggcggag gtacagatac ctctggaaac
    14521 aactctggag gcaacggcaa taacaaagag gaattaactt ttccaattgc aacaaaaaat
    14581 aatgatgatg caatcgaaga cggcgaatta gaagctgcgg tcgcaacgga cagtcagttt
    14641 aaaggattgt tccagtggga gttttaccaa gattcatatg atgcagcttt tatggctcct
    14701 tctcatgaac cgctatttgc aaacgatgac gatttcagaa tcacagacga cggagctgca
    14761 acgcttgatc tagatgaaga tacgaaaaaa gcgacaatca aagtaaaaga caacgtcaaa
    14821 tggtcagatg gtgaagatgt aacggcggac gatgtgattt tcccttacga aatcattggg
    14881 aataaagatt acactggtat ccgttatgac gacactttcc gtaacatcgt aggtatggaa
    14941 gaatatcatg atggaagtgc cgacacaatc tccggaatca aaaaaataga cgacaaaaca
    15001 gttgaaatcg aatataaaga agtaaaccca agcatgcttc aatcgggtgg tggtatctgg
    15061 tcatatgcag caccaaaaca tacgttagaa ggtgtagcaa tcaaagatat ggaatcaagc
    15121 gatcaagtac gtaaaaatcc agtgactttt ggcccttatt acatgagtaa tatcgttgca
    15181 ggagaatcag tagaatactt gccaaacgag cactactgga atggtacgcc taaattgaaa
    15241 aagatcacga tgaaatctgt accgatggca agtgcaactg aagcattgaa ctctaaatta
    15301 tatgatatga ttttccaaat gccaacagat acttacgata cctataaaga aattgctggc
    15361 tatacaaact taggccgtga acaaacttct tatacttatc taggattcaa gttaggtaaa
    15421 tggaatgcag atacaaagag tgtggaatac aatccagatt caaaaatggc tgataaatca
    15481 ttacgtcaag caatgggcta tgcattagat aataatgcag taggtgaacg tttctatgca
    15541 ggtttgagaa gcaatgcgac ttcattgatt cctccagtat tccagtcata ccatgatgat
    15601 gagatgaaag gattcacaca agatctagac aaagcgaaaa aactattaga tgatgctggc
    15661 tacaaagatg tggatgacga tggtttaaga gaagacaaag atggaaataa actaacaatc
    15721 aactttgctt cgatgtccgg tggggaaaca gcgcaaccat tggcagatta ctatatccaa
    15781 caatggaaag aaattggttt ggatgtccaa tacacaacag gtcgtctgat cgaattcaat
    15841 tctttctatg ataaattaga aaacgatgat cctgaaattg atgtttacca agctgcttgg
    15901 aatacaggaa ctgatccaaa tccaagtgga ctatggggac caaatgctgc gttcaactac
    15961 actcgttatg aatcagaaga aaatacaaaa ttgatcaacg atatcagttc aagcaaagca
    16021 tttgatgaag agtaccaaaa agaagcatat caaaaatggc aacaatatgc atttgaagaa
    16081 gcatttgcga ttccaacgtt gttccgtaat gaagtattgc cagtcaacga ccgtgtcaaa
    16141 gactgggatt ggtcttatga tgtaccaaac ccatgggcaa atgtttctgt tacagcagat
    16201 tcacgttcat aatcactaaa aatgaccgaa aaagggcgat gtcctttttc ggttttttga
    16261 tatttttctg tttatttctc taatattcag gtcaaggcgc tgatttcatg acttctccaa
    16321 taaacggtgt tcaaagtcca gtttctcggt attaagtcaa atttatgcaa aaatatggaa
    16381 agccattttc acaaaatttt ttcttcatac tcgaaactaa acgaattttt cacaacttct
    16441 cgtcaatcac taggattatt aaagctttac ttaccttttg accgaaccat ttcaaatttg
    16501 acaaaaaacc agtataataa ggaagtatcg aaaaaatatc cataaaagga atgtggaaaa
    16561 tggacaacca gctgacaaaa gaactaaaag aaaactataa catcgttttt cacgatctaa
    16621 atttgcttga acaagctttt acccattcat cctatgtgaa tgagcatcgc aatctgcaat
    16681 tatcggataa tgagcgactt gaatttttag gagatgccgt tttagaactg atggtttctc
    16741 agtatttatt ccgtttatat cctcatttgc cagaaggaaa attgacaaaa acacgagctg
    16801 cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga atgtcacttt gatcaatatg
    16861 ttttgttggg caagggagaa gagaattcag gaggacgtac gcgaccagca ttgctttgcg
    16921 acctattcga agctttttta ggcgcgttat atcttgacca gggattcgac acgacccatg
    16981 cattcatcga aaaagtgatt tttcctaaag tcaaagcagg tgctttttca catgagatgg
    17041 atcataaaac aaaactacaa gaagtcttgc aaaaatcagg tgatgtatca atcgaatacc
    17101 gattgatcaa tgaagaaggt ccagctcatg aacgagtatt ttggatcgaa gtttatgtag
    17161 atgatcaatt gattggcact ggacaaggga aatcgaaaaa attagcagaa caagctgcgg
    17221 cggaaaatgc attggcagca ctgtaaaagt aagtgaggga aagctttgta tttgaaacga
    17281 attgaaatag ccggttttaa atcatttgca gacaagacag tcattgattt tgaaaatagc
    17341 gtcacagcag tggttggacc aaatggcagc ggaaaaagca atattacaga ggctatccgc
    17401 tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg gaaaaatgcc ggatatcatc
    17461 tttgccggtt ccgatacccg taagcagctg aatattgcag aagtcacagt gattttagac
    17521 aatagtgatc attatctgcc attggagtac aatgagatca gtgtaacccg ccgttatcga
    17581 cggactgggg aaagcgagtt tttcatcaat aaacaaagct gcagattaaa agatatccaa
    17641 gaattgttct tagattctgg attaggaaaa gaatcgttct caatcatttc tcaaggaaaa
    17701 gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg ggatctttga agaagcagct
    17761 ggtgtcttga aatacaagca gcggaaaaag aaagcagaac agaagctttt tgaaacagaa
    17821 gacaacctca gccgcgtcca agacatcatc cacgaattgg aagaacagtt aactcctttg
    17881 gccgcccaga gtgaggcagc aaaggagttt ttacggctaa aagaaacact gactcaaacc
    17941 gatgtctctt tgatggttgc tgaaatcaag acagcaaaaa aagattggga caacaaacaa
    18001 gcacagcttg cgaaattcaa tctagaatta ggcaaactat ctgagtcgat ccaagaacaa
    18061 gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag ccgatcgtct gatcgaaaaa
    18121 aatcagcaag tattattaga tctttcggaa aaactgaagc agacagaagg acaaaaagat
    18181 gttttgcagg agcggacaaa gcatacacaa aaaagctcgc aagaatatca aacttcatta
    18241 gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc aagaaagctt gatgaaagca
    18301 gcagcagaaa aagaaacaga gatccaaaaa gctgaagcaa atctcataaa aacacagcaa
    18361 gaattagaaa aataccaaaa atcaacgaaa gaattattgg cagaattaag agatcaatat
    18421 gttgatttga tgcaagaaca agcagctgta ggaaacgaac tgaagtatct agaacgtcaa
    18481 tatatacaag aaacagcaaa aagcaagcag acgttagcca agcaatcgga agtagaagca
    18541 tcagtagacc gattgatctt acaaaaagaa gaattgacac aaaagcaggc gcagctaaaa
    18601 tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc aacaaaacgg gaaaaagttc
    18661 caagaagcat tagcaaaaga acagcctaaa atgtatcaat taatgaatca agtccagcag
    18721 ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa actattttgg tttttaccaa
    18781 ggggttcgat tggtcttgca gcataaacag cagctttcgg gtattgtcgg agcagtggct
    18841 gaactgatcg atgttcctgc agatttcacg ttagctatcg aagcagcttt aggtggggca
    18901 gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc aagccatcac gtatttgaaa
    18961 cagcaaagag gaggaagagc aacattcctt ccactgacga caatcaaacc aagacaatta
    19021 ccggctcata tattgacaca agcagcagct gtagaagggt ttatagggat tgccagtgaa
    19081 caagtcagtt atcccgatca aatccaaact gtggttcaaa acctgctagg aacgatcctt
    19141 ttagccaaag acttaactag tgccaatgca atcgcgcaaa cgatccgcta tcaatatcga
    19201 gtggtttcct tagaaggtga tgtgatgaat gccggaggat ccatgacagg tggagcgaat
    19261 aaacgaggaa accaaggcag tttgtttgta caaaatcaag aattgaaaca gttgactagt
    19321 gaatttgaag aagcagataa acaactccaa gcacaagaaa aaaaagttca ggagctgcaa
    19381 caagaaacag ctcgtttagc cgaagaacaa gaagttttgc gtacacgagg cgaacaatta
    19441 cgttttgaag aacaagaagc gacaaatcag ctacaaaaca tcataaatga acttgaacgt
    19501 tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg aactgcagca gtttatcgaa
    19561 gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa cagatcttga atcgcaacga
    19621 caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa gcgaccagat ggaagccaga
    19681 agagcacaag tacaaagtca aaaagcacaa gaacaagcag atcttgctgt cttgaaagag
    19741 cagtttaatc atctgcagat ccagctccgc ggtgcccgag tccaaaaagc agaggcaacc
    19801 gaacgtcaag aagcgataga aaagcagttg gcgacattga cagcagactt ttctgatcat
    19861 gaagtcacag aagaaagttt ggaaaaacaa attaacgaat tatctgctca gcgagaaaca
    19921 ttaaaagccg agttggcaaa agccaaagag cagcgagata gaaggcaaaa ggagatcgat
    19981 aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa aagcacgact gtcggaacaa
    20041 tccaaattag aagtccaaaa agaccgagct gaaatgattt tagataatca tttgacctat
    20101 ctgcagtcag aatatcagct gacatttgag aaagcttccc aagactatca agaaacaacc
    20161 gatatcgaag acagtagaac aaaggtttca tccttaaaag aacagattga aaaactggga
    20221 ccagtaaact taaattcaat cgaacaatac gaacaagtca gtgaaagaca tacattctta
    20281 gcaacacagc gagatgatct gttagcagcg aaaaaccaat tgtttgaaac gatggatgag
    20341 atggatgatg aagtgcggac aagattcaaa gaagtatttg aagctatccg tcaagaattc
    20401 aaaattgtct ttccaaatat gtttggcggc ggtaaagctg aattagtact gacggaacca
    20461 actgatttgc tgaaaacagg tattgagatt gaagtacaac ctcctggcaa aaaactgcaa
    20521 agtctaagtt tgctttctgg aggagaacga gcacttacag ccatcgcgtt gctcttttcc
    20581 attatccgtg tctgtcctgt tccgttttgt atcttagatg aggtagaagc agcgttagat
    20641 gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt tccaagatga cacacaattt
    20701 atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag atgtactgta cggagtgacg
    20761 atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga tggaagatat caatgaagag
    20821 gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat caaattagta gcaatcgatt
    20881 tggacggtac attattaaat gaacaaaagc tgatttcaga tgagaataaa caagcattag
    20941 cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac aggacgcccc ttagctgcga
    21001 tggcgcatta tctgcaagaa ttaggactgg tagatgaagg tgactacagc atcactttca
    21061 acggcggttt ggtacaaaaa aatgatactg gtgaaatcat ggaaaagaaa gtgatggaag
    21121 tatcagatat ccaacgctta tacaaactgg ctcagcaatt ggatctgcca ctagatgttt
    21181 tgtctgacaa tgtggtttta cagctgccta gtgccccaag gaagcaatcc ttgtacaatg
    21241 tgttgaataa gctgctgcaa ttccaaccag cggatataag cgatattaca gatgaatttg
    21301 tgcttaataa ggcagtaatc gcttatgacc aagaagaatt agatcctaaa atcaaagaaa
    21361 taccagcaga attccatgaa atgtatgaga tcatcaaaac aagaagtatg cttttggaat
    21421 ttatgccaaa aggtgttaca aaagcttatg gtatttcttt attggcaaaa gatcttggtt
    21481 tgaaacaaga agaaatcatg gcgatcggtg atgaggaaaa tgatttaccg atgatccaat
    21541 atgctggact aggcgtagcg atggagaatg ctgtgccttt cgtcaaagaa gcagcagatt
    21601 tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt gatccaaaaa tttgtattag
    21661 agtaggggga gtcaaatggg attttttgat aaaatcaagc gtgcttttac tggcgaagag
    21721 aaaacagaag aagttgaagt acaggagaag tacgacaagg gtttagaaaa gacgcgtaaa
    21781 acatttggtg aacggatgaa tgagttgttt gccaattttc gaacagtcga tgaagacttt
    21841 tttgaagaat tagaagaaac acttatcggt gcagatgtag gttttgaaac agcgatacga
    21901 atcaccgaag cattacgtca agaagtgaaa ctcagaaatg cgaaaaaatc ggcagaagtc
    21961 caaaatgcga tcatcgagaa acttgtggat ctctatgaag cggaaggaat caatgaagta
    22021 aatgaactaa atatccaaga agacggacta acagttattt tgtttgtcgg tgttaatggt
    22081 gtagggaaaa caacaagtat tggtaaactt gcccatcagt tcaaaaacga aggtaaaaaa
    22141 gtattgatgg cagcagcaga taccttccgc gctggagcaa tcgaccagtt agttgtctgg
    22201 ggcgaacgcg ctggtgttga agttgttcgt ggcaatgcag gaggcgatcc agcagctgtc
    22261 gtattcgatg ctttggaaag agcaaagaca gagcaggcag atgttttact agttgatact
    22321 gcaggccgac tgcaaaataa agtgaacttg atgaacgaat tagaaaaaat aaaacgagta
    22381 atccagcggg aattaccaga agcaccccaa gaaacacttc ttgtattaga tgcaaccact
    22441 ggtcaaaacg cgatggttca agcaaaacaa ttcaaagaaa caacagatgt gaccggtctt
    22501 gttttgacaa aattagatgg tacagcaaag ggcggtatcg tacttgctat ccgtaatgaa
    22561 ttgcatttac cagtgaaatt ggtcggtctt ggtgaaggaa tcgatgactt ggagccgttt
    22621 gatccaaatg attttgtcgt tggattgttc aaaggattac taaaagaaga ataacccatg
    22681 tctaaaaagc aggagctgtg acaaaagttg cagctccttg tttgaatgat tgtttttaac
    22741 atagctgtgt ttttgatgcc ttcattttta tacttatttg taagcggttc tcaaacaatt
    22801 tcgttctttt tcttagactt tttgattata attagattca tatgaattgg aggtcactta
    22861 atgatgaatt ttaagcaatt attattgcat gtcaatgcgc gcccttttat cgaccaagct
    22921 cgatttggta tagaacggga aggacagcgg gttgatcttg caggaaatct agcaaaaacc
    22981 gatcatccag caatctttgg cgatcgatcc tatcacccct atatccaaac agattttagc
    23041 gaaacacaaa cagagatgat cacccctgtt accgattcta ttcccgaatt atttcagtat
    23101 ctcgctgctg tttatgatgt gactgctcgt tctataccga aagaagagat gatctggcca
    23161 ttaagtatgc cacctgcctt accggaaaaa gacgaagaga tcattattgc aaaattaaaa
    23221 aatttcgaag atgtcttgta tcgacgttat ttagcaaaag aatacgggaa gcgaaaacag
    23281 atggtaagcg gtattcattt caattttgaa tttggcgatg agttgctaag aacattgttc
    23341 agccatcagg aagaatttca agatttttcc gaattcaaaa cagaacttta tttgaaaaca
    23401 gcgagaaact ttatgcgcta tcgctggatg atcacttatt tattcggtgc ttcaccaatg
    23461 agtgagaaga actacttttt agatgaatca catccgcaag aacctgttcg cagtattcgc
    23521 aacagtgcgc tgggctatac gaatcatcca aatgtgaaag tttcctatgc ttctatgaaa
    23581 cagtatttag cagatatcga gcgaatgatc gaagaaggga aactttcgga ggagaaagaa
    23641 ttttatacac cgcttcgttt ccgaggcgga aagaaagtcg cagatttagc aacaacaggt
    23701 gttcgttata ttgaattgcg gaatatcgat ttgaatcctt atgcaagatt gggtatcaat
    23761 ccagagcaag ttcggttctt acaattattc ctgatgtaca tgttatggac agaagaaaaa
    23821 gaagactgtg accaatgggt agcagaaggt acgactcgaa ataacaaagt agcactagaa
    23881 caaccatcag atcaaacaga atttcatcaa gaaggtagag aaattcttga aggaatgaag
    23941 caaatgctgg ttgaattgga ttggttggat tctctttatt tagtagagga agcattgact
    24001 caaatggatc atcctgaaca aacgttagca gcgaaactct atcaagaagc acagctatcg
    24061 agtcagcaag aagttgctgt ggcattggga catcaatatt ataaagaaag ccatgaacgt
    24121 ccctatcagt tggccggttt tcgcgaaatg gaactttcta ctcagatatt tatgttcgat
    24181 gccatccaaa aaggtgtcca agtcaaagta ttagatgagt cggatcaatt tttgcgtctg
    24241 caattccaag accatgtaga atatgtgaaa aatgcaaaca tgacaagtaa ggacagctat
    24301 atcgtcccac ttatcatgga aaacaaaaca gtaacgaaaa aagtcttaaa agaagctggg
    24361 ttccgggtac caggaggcgc agaattttca tccatggaag aagcggtaaa agcttatccg
    24421 agatttgccg agcaggcatt tgtgatcaaa cctaaatcaa ccaattacgg gttaggtatc
    24481 acgattttca aggaaggagc tagtctggaa gattatcaag caggtttagc cattgctttt
    24541 cgtgaagaca gttcggtttt agtagaagaa tttatgccgg gaacggaata tcgtttcttt
    24601 gtgatcgacg gagaagtcca agccatcatg ttgcgagtcc ctgctaatgt tattggtgac
    24661 agtatccgaa cagtgaaaga acttgttgaa gaaaaaaata gtgacccttt gcgtggaacg
    24721 aatcatcgtg cacctttaga attgatccaa ttaggtgaat tagaacaatt gatgttgaaa
    24781 gaacaagggt tgacgatcga atcagttccc caagccaatc aaattgttta tctaagggag
    24841 aactcaaata tcagtacagg tggtgattcg atcgatatga ctgatgaatt ttctgaagct
    24901 tacaaaaaaa tcgccgtttc tgcggtagaa gcactaggag ctaagattag tggtatcgat
    24961 ttgattatac cagataaaga aatcgatcct acaactgata aaaaagcata tgggatcatc
    25021 gaagcaaatt tcaatccagc catgcatatg catgtttatc cttttgcagg aaaaggcaga
    25081 agattgacga tgaatgtatt gaaattgtta tatcctgaag tattttaaaa gaaaaagctt
    25141 ttgacagatt gtcctatttg atgtcaatct gatcaaaagc tttttttgca tgtaaaaatt
    25201 tatgtgtttt ttcgttttaa aataaacgat cccagaagct ttttttcttt tttggttttt
    25261 ctttttcagc tggcgaagag ttttcttctg ggtatttttt ttcgatatca atcacaggtt
    25321 catttactgc ttcttttgct gttaagagca atccgaattc ctcaggattt gcagtgacgt
    25381 aatcgttgac tacagtgaaa cggctattgt ttttcgttgc tagctggata tattggtttt
    25441 ggacagaaag gggtagagcc ccattcaaga gtatggaagc atctggatgt tttttgagtt
    25501 cttttaaaaa gttttctttg ttttttggtt ggatcatttc agcaacagtc atactaagat
    25561 agcagcgttc cctgaatgtc cctaaatatt tatgctgttc ttcagggttc actaaaggtg
    25621 taccataaag tcccttatcc aaatggcttt ttacatcatc ttgtgccata aaaaataccc
    25681 tccttcttct ctttgtcttt cttacgatta tagcatattc ccaataatac ctaaaacata
    25741 tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat aagaatgaaa atcattctca
    25801 attagaaagg tggattggat gcgctacgga cgactagtag cattattagt tattttaagt
    25861 agtatttcat tgttcatcgg agtatcgaat gtacaattgt cagaactgct ccagttagat
    25921 gggcaaagct tacaaatctt gctagtgact cgtttgccac gaacgatttg tttgatcctt
    25981 gtgggagcga ccagtagtat ttgtgggttg atcatgcaac atttgaccca aaataaattt
    26041 gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt taggtatttt agtggctatg
    26101 attttcctac ctggtgcatc cttgattgtc cgatccttga cagcattttg ctttgctttt
    26161 gcaggaacat tgcttttttt atcccttacg cgattgttcc tccagaaaaa tcaattgatg
    26221 cttccgttga ttggagtcat gttagggaat atcattggtt cagttgctac tttttttgct
    26281 tatcaattcc agttggtaca aaatatgact tcatggcttc aagggaattt tgcgactgtg
    26341 atacgaggaa attatgagtt attgtatgct gttgttccat tgttgctctt ggtttggcta
    26401 tttgcttacc gatttacgat cattggtctg ggcgaatcgt ttgcagtaaa tctgggaata
    26461 gattaccaga agatgcagtt tttaggcatt ggcttagtat cattggctag tgcagtcatg
    26521 ttgatcatgg taggaagcat tccttttatc ggagtgatca ttccgaatct tgtctctaga
    26581 atatatggag atcaagtgca caaaacaatt ggcgtaactg ctttagcagg tagtttgttc
    26641 ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc cttatgaaat accagtcagt
    26701 gtgatcgttg gaatcatcgg tggggctatc ttcctttatc ttctgataag gggacgacga
    26761 tatgcttaaa aaaaagccat ctatttttat tggtatgctt gttcttgttt tatttgttac
    26821 aggaatgtac ttgacgatcc atacatatgg aaactggcat tttgcatgtg cattgaggag
    26881 taagaaaatt tttgcattta tccttgtctc gctagctgtc agcagtgcca caatcagctt
    26941 tcagacaatt acgcataacc agtttttgac tccttcagta ttgggacttg agtcgttata
    27001 tgtccttgtc caaacggtcc ttttcttctt tatcggagga gtgacgatgc ttcagcagac
    27061 aggtacagtt tttttcttat tgaacgttgg gctgatggtc gggctgagtc tattgttatt
    27121 tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta ttactgatgt cagggatgat
    27181 tttaggtacg ctttttggta gtgtcagtac ctttttacaa gtcatcatgg atccgaatga
    27241 atatgatttg cttcaaggca gattatttgc aagctttggc aatatccaga gtcagtatct
    27301 tttgcctgct tctttgatca ttatcggctg ctggtacctg ttatttcgat atcgacatgt
    27361 gctagacgtt ttccatctcg gaaatgaaca agcagtaaac ctcgggatag acacagatcg
    27421 ttttgaattt ttgattttat gttgtgtcag tcttttgaca gggacatcaa cggctcttgt
    27481 gggtccagtt acttttttag ggtttattgt cgcaaatatc agttatcgtg tcttttctac
    27541 ttatcgtcat ggtcagctat ttatcggaag ctttctggta gctatgctgt ttctgacagg
    27601 aggacagctc gcagtagagc agattttcgg ctggaataca acagtgagtg tcgtcgttga
    27661 atttgtcgga ggaatttatt ttgtcacaaa attgattcgc gaaaggaaga gtatcctatg
    27721 attgatttac aaaaggtcaa taaactgtac catggaaaat cagtggttaa agatgtacag
    27781 ttaacgattc ctgaaaagaa gttgactgct tttatcggtc caaatggtgc tggaaagtca
    27841 acggtgttgt cgatgatcag tcgtttgata ccaaaagata caggtgaaat ctatttagac
    27901 cataacgaag taaaggcttg gaaatccaat gaattagcca aaaaattatc gatcctacga
    27961 caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat tagtagaatt tggacgattt
    28021 ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa tcgttcagga atcactggaa
    28081 cacattgggt tagaagaaat agcggatcaa tcgattgaca ctctttcagg cggtcaactc
    28141 caacgtgcgt atattgcaat ggtgctagcg caagacacgg attatatttt actggatgaa
    28201 cctttgaaca atctagatat gaattttgcg gttcaaatca tgcagatttt aaaaaattta
    28261 gtacaagaaa tcgggaaaac aattgttatt gttcttcatg atatcaactt tgctgcaagt
    28321 tatgcagacg aaattgttgc aatgaaagat ggacagttat ttacgcaagg aacgacccaa
    28381 gaaatgataa aaaaagatgt attggatgaa ttatacgaaa tggacatacg gatttgtgag
    28441 attgaaggcc gcagattctg tctttatttc taaaactaac attaggagag aagagatgaa
    28501 aaaattagta gtaggtatct tgattctagc atcatttggt ttagcagcat gtggaaatag
    28561 caacaacacc agtcaggcag acacaaaaaa aagcagcaca caaactgaaa ctacattgac
    28621 gataacagat agtaatggag accaaatcga ggtgccaaat aatccagaaa aagtcgtcgt
    28681 attcgacaat ggttctttag atacgatgga tgctcttggt gttggagata aggtagttgg
    28741 agcagctaca agcagccttc ctgagtatct ttcatcctat aaaaaagtag aatcagcagg
    28801 tggaataaaa gaaccagact tagaaaaaat taatcagctt cagccagatc taatcatcat
    28861 ttctggccga caaagagatt ttcaaaaaga tctaagtcaa attgctccga ccattttcct
    28921 ttctttagat gcgaaaaatc catgggaatc tttccagcaa aatgtcactg ctttagggga
    28981 gatctttggt aagcaagaag aagctaaaac tcaattagag gaattatcta gtgcaatcga
    29041 tcagacgaaa aagaaagcag aagctactga caaaaaagca ctagtcacac ttgtgaatga
    29101 agggcaatta tcagcctacg gatctggttc tagattcggt ttcatccatg atttatttgg
    29161 ctttgaacaa gcagatgatc aaatcgaagc atccactcac ggtcaaagtg tttcttatga
    29221 atatgtgctg gaaaaaaatc cggatatctt atttgtagta gacagaacaa aagctattgg
    29281 tggagacgac agcaaagatg atatttctgc caatgaactg gtagcacaaa cgaatgctgg
    29341 taaaaatcag cagatcatct cccttgaacc agatgtctgg tatttaagcg ggggcggttt
    29401 agaatcaatg aaactgatga tcgaagatgt gaatcaagca tttaaatagc ataaataaca
    29461 gaggattaaa ctctcatact atgtaacaat aatgtttctt acattcttaa atagcaagag
    29521 cctatcctca aagaagttat caaaaaccat ccgtaaaatc gtattgatga ttttacggat
    29581 ggtttttatt tatttgagaa atcttttttc aacgtgaatt cattatctac atcatacggg
    29641 ttgattctag aatggggagt ggcaaaaaat agtgtgatcc attttttcag catcaagatc
    29701 gcctcctttg atttgaatca aaggtacaaa gcgaaactta gataaaactt aaatttagat
    29761 atttttattt tgtaccgatc tcttctttaa acgatgttcc atctgctgct tctcggcaat
    29821 aagtccacta gactagaaaa tatgagaagc tattttctgt ccagcgttct tattactcaa
    29881 aacaaatcgc agttgtcaca atctcttcta aacgatgttc catctgctgc ttctcggcaa
    29941 taagcccact agactagaaa atatgagaag ctattttctg tccagcgttc ttattactca
    30001 aagcaaatcg cagttgtcac aatctcttct aaacaatgtt caatcagctg accttcggta
    30061 ttagctcaaa gcactgattt cacaacctct atttaggcat gtgtctcagc aagcggttga
    30121 tagttgatga tctgcatttt tcgataaaaa tcggcgggaa gcttactcca ccgttttagt
    30181 aattgataga aataactctc ttctgctttg attttatact cgcttaatat caatccaaaa
    30241 ctattcgttt tttttgaagc taatttttta taaatatcca atgctttggt tttatcttca
    30301 ttgacaaaga gtgtttttac ttcaatataa gaaccctcga tattttgtgt ttcttcgata
    30361 aaatagatgt tttttatata ttccttcata gtgattcctc caaagaagtc ttttatttga
    30421 taagtttact ctaccgaaga gttgttaata atccgtgaac aaaaagtaat tctttttcca
    30481 atagttgtta aaggtttttt attttttata gtattttttg ttatttaaga aaggaataag
    30541 gttaaacaaa taacaggttg ctttttgggt ctttttccgt tcaagaaact gaacgaagaa
    30601 aagggaaagt tgtctctttt gtgataagag aaatcataat aaacgaatga cttactttcc
    30661 aataaattga gaacagtcag aaccagacaa attagttgca tctgatgaat aaagtgatta
    30721 ttctatgaca tgaggtgata cagatgaaat tagaagtgac aaaagaagca caagaagtat
    30781 tgaagaataa attagaagca gacgatcaac ttttattaga tatcgaaaac ggtgacggtc
    30841 catttgcaga aagccaagtc agttgccaat tggatacggc atttcgattg atcattgtca
    30901 aaaaagatac acaaacagat ctaagtcttt attctgtagt ggctgataca ccagttggac
    30961 caatcaaaat caaagacagt gcaatacttt acatggatga tccatccaca cttgctttag
    31021 aaccaactta taatagttta caattaaaag gaccaagcgg cttgagaaaa ggcaacttgc
    31081 aagttgttcg aaaagactag agggataaaa tgggaaaatt ggcgatcatc agcgacctac
    31141 atgtggacat caataaattc ggcgaatcag agctgatgca gctttggcaa gttttgcaaa
    31201 agcacaagat cactcggctt catctagccg gagatacggc taataaagta gaccgttgcc
    31261 tagctgttgt cgaatttttc gtcaataaag gattatctac gacatttcat ttcggcaatc
    31321 acgagttggc agatgtgccc aatgaacaga tgatggacca ttttccagac ccacattttt
    31381 taaatgaacg gtatattgct ttaaatgagc agaccgttct tttagggatg aatggctggt
    31441 acgactatca gttttccgaa ttgaaagatt acgaggcaat cgtacgaatg aagcgtttgt
    31501 attggtatga tcggttcatc cagcgtggga aaacagatct cgaggtcaat gcagaaatgc
    31561 ttgcagctgc aaaaaatttg ctagaccagc tggaagcaaa aaaacttgag gtgattttat
    31621 ccactcattt cgttcctaaa cgggcattta tcatgtatca atctgctcct tatgagcgtt
    31681 ggaacaaatt aaatgcattt ttaggatcag aatcattcgg agaattactt gaccgttatt
    31741 caaatatcaa acaagtagtt tttggccata cgcatcgtcg ctttgaagat aaatccatcg
    31801 gtcacacgat ttacagctgc agaccttttg gttattatta tgaatggcag ctgacccgag
    31861 attttgtctt gaaagaacag ttgctagaca gctatgatcc tatgaaatta cgtgtcctct
    31921 tgcgtgccca tcacccagct tttcaggcct atcaaacaca acatttgcaa gaggaatttg
    31981 aacaagcgat gacaatcgtt ccgtattgag acagcattag taatgtgtaa aagaaaatac
    32041 cttgtaaaac ataaggataa gtaagagcag tagatccatt tatcgtggat atgctgcttt
    32101 ttttgcggaa aataatcagc gaatgtttat ttgatcggat gatttgtaac tcgcagagta
    32161 ctagatgcaa gcgtgtattc tttttttgaa cgataatcac aaaaaaaaat ataatataca
    32221 ttttttattt aattataatt aatattgatt gaagtctagg cagtcaagtc gagagtgtaa
    32281 aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa gtc
//
LOCUS       DASNNU010000024        32189 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.024, whole genome shotgun sequence.
ACCESSION   DASNNU010000024 DASNNU010000000
VERSION     DASNNU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.024"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..368
                     /locus_tag="U8116_001049"
     CDS             <1..368
                     /locus_tag="U8116_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN8434058.1"
                     /translation="FTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVGV
                     EDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLGE
                     EYVFNFADPDGNYFAVTGK"
     gene            548..1489
                     /locus_tag="U8116_001050"
     CDS             548..1489
                     /locus_tag="U8116_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEN8434059.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1558..2289
                     /locus_tag="U8116_001051"
     CDS             1558..2289
                     /locus_tag="U8116_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEN8434060.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2433..4613
                     /locus_tag="U8116_001052"
     CDS             2433..4613
                     /locus_tag="U8116_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEN8434061.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4633..5115
                     /locus_tag="U8116_001053"
     CDS             4633..5115
                     /locus_tag="U8116_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEN8434062.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5131..5892
                     /locus_tag="U8116_001054"
     CDS             5131..5892
                     /locus_tag="U8116_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN8434063.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6023..7357
                     /locus_tag="U8116_001055"
     CDS             6023..7357
                     /locus_tag="U8116_001055"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEN8434064.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7664..8149
                     /locus_tag="U8116_001056"
     CDS             7664..8149
                     /locus_tag="U8116_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEN8434065.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8142..8825
                     /locus_tag="U8116_001057"
     CDS             8142..8825
                     /locus_tag="U8116_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEN8434066.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8951..11596
                     /gene="polA"
                     /locus_tag="U8116_001058"
     CDS             8951..11596
                     /gene="polA"
                     /locus_tag="U8116_001058"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEN8434067.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11641..12477
                     /gene="mutM"
                     /locus_tag="U8116_001059"
     CDS             11641..12477
                     /gene="mutM"
                     /locus_tag="U8116_001059"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEN8434068.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12441..13070
                     /locus_tag="U8116_001060"
     CDS             12441..13070
                     /locus_tag="U8116_001060"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEN8434069.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13122..13454
                     /locus_tag="U8116_001061"
     CDS             13122..13454
                     /locus_tag="U8116_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN8434070.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13766..14257
                     /gene="nrdR"
                     /locus_tag="U8116_001062"
     CDS             13766..14257
                     /gene="nrdR"
                     /locus_tag="U8116_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEN8434071.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14280..15653
                     /locus_tag="U8116_001063"
     CDS             14280..15653
                     /locus_tag="U8116_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN8434072.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15653..16579
                     /gene="dnaI"
                     /locus_tag="U8116_001064"
     CDS             15653..16579
                     /gene="dnaI"
                     /locus_tag="U8116_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEN8434073.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16708..17148
                     /locus_tag="U8116_001065"
     CDS             16708..17148
                     /locus_tag="U8116_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN8434074.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17292..17993
                     /locus_tag="U8116_001066"
     CDS             17292..17993
                     /locus_tag="U8116_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEN8434075.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17990..19111
                     /locus_tag="U8116_001067"
     CDS             17990..19111
                     /locus_tag="U8116_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEN8434076.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKINLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLYKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19126..20358
                     /gene="pepT"
                     /locus_tag="U8116_001068"
     CDS             19126..20358
                     /gene="pepT"
                     /locus_tag="U8116_001068"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEN8434077.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20490..21398
                     /locus_tag="U8116_001069"
     CDS             20490..21398
                     /locus_tag="U8116_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEN8434078.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21433..21705
                     /locus_tag="U8116_001070"
     CDS             21433..21705
                     /locus_tag="U8116_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEN8434079.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21716..22762
                     /locus_tag="U8116_001071"
     CDS             21716..22762
                     /locus_tag="U8116_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEN8434080.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23013..25622
                     /gene="clpB"
                     /locus_tag="U8116_001072"
     CDS             23013..25622
                     /gene="clpB"
                     /locus_tag="U8116_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEN8434081.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25773..26459)
                     /locus_tag="U8116_001073"
     CDS             complement(25773..26459)
                     /locus_tag="U8116_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434082.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26437..26931)
                     /locus_tag="U8116_001074"
     CDS             complement(26437..26931)
                     /locus_tag="U8116_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN8434083.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26951..28171)
                     /locus_tag="U8116_001075"
     CDS             complement(26951..28171)
                     /locus_tag="U8116_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8434084.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28318..30153
                     /gene="lepA"
                     /locus_tag="U8116_001076"
     CDS             28318..30153
                     /gene="lepA"
                     /locus_tag="U8116_001076"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEN8434085.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30349..31260
                     /locus_tag="U8116_001077"
     CDS             30349..31260
                     /locus_tag="U8116_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8434086.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT        10886 a         5596 c         6902 g         8805 t
ORIGIN      
        1 tgtttacgaa tgaaataaaa atcatgcttt atgtggacga tgtagaaaaa aatgcaacgt
       61 tttggagagc agttggcttt gcggaaaaag atcgtcaaga aatggatggg acgcttataa
      121 ttgaagtagg agttgaggac agtcaaacct catttctgtt atatgatcga gcatttgttg
      181 agtcccattc acctggcaca gctactagtg ccccatcttt gatgttttac agtaaagata
      241 tctttggact atataaaaaa atgcagacgc aatcagtcac attaggtgat atggtacagc
      301 ttggagaaga atatgtcttt aactttgctg atcccgatgg caactacttt gcggttacag
      361 gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa caggaaagcg aagtcttttt
      421 aattcaaata ataatattat gtaaacaaaa aactttccat gcgtaattat acacaagcaa
      481 ataaatcgtc aaatgcgaac gccttcttta tactgtttag gaacgaaaag aaggaaggaa
      541 gaaatacatg gattatcaag tactgcttta ttacaattat acaaaaatcg aagaaccaga
      601 ggaatttgcc aaaaatcatt tagccttttg caaatctttg gatcttaaag gtcgtattct
      661 agtagctgaa gaaggaataa atggtactgt gtctggaacc gttgaaaaca cgactgccta
      721 tatgaaagca atgaaagctg atcctcgatt tgctgatacg atgttcaaga tcgatccaag
      781 cgaaggacat gcctttaaga agatgtttgt aagataccgt ccagagcttg taagtttaaa
      841 gctagaagag gacattgatc caaaccaagt gacaggtgcc tatctttcac cgcaagaatt
      901 taaagaagct attttagatg aacagacagt ggtgattgat gcacggaatg attatgaata
      961 tgacttggga cattttcgag gggctgtacg cccagatata cgcagttttc gagagcttcc
     1021 agactggatc cgcgagcaca aagaagaatt tatggaaaaa cgggtagtga cctactgtac
     1081 tggtggcatt cgttgcgaga agttttctgg ttggcttgtc cgtgaaggat tcaaagatgt
     1141 cggacagctg catggcggta ttgctactta tggtaaggat cctgaagtcc aaggagaatt
     1201 atgggacggc aagatgtatg tgtttgatga acggatcgct gtcgatatca accaagtaga
     1261 taaacaaatc gtcggtcgcg actggtttga cggaacacca tgtgaacgat atatcaattg
     1321 tagcaatcct gagtgcaatc gtcagattct tgcttccatt gaaaatgaag aaaaacattt
     1381 aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat cgctatgttc aagaccatgg
     1441 aatctctcca gaagaagcag aactgcggtt tacagcttta attaaataga aaacaatagt
     1501 tttgattgtc gaccatttcc aatatgcgct atactagcgt tgaggatggt gatccacatg
     1561 aaatcattga tttatgctat tggggatgtc catggcaaac atgagctttt tgaaaaactt
     1621 attcactatt atgatccaaa gatccatcag cttgttttat taggggatct caatgatcga
     1681 ggtcctcgta caaaagaatg ttttttatta ggaaagcaat tagtcgaaga aacaggagcc
     1741 atttacctcc ggggaaacca tgaagaatat tttttgcaat ttctgcaatc accggaggac
     1801 tggtatgcgc cttatatgag aaatggtgga aaagaaacgt ttgaaagcct gattcaccca
     1861 ggggcatcgg aggaatactc cccaactgaa atctcgatga tgatccaatc gagatattca
     1921 gaactgatta aatttttagt ccagcgcccc ttgtattatg aatgggaaaa ttatcttttt
     1981 gtccatgccg gtgtggattt gacaaagaaa gactggcgta agacaagtct aagagacttt
     2041 atttggataa gggaaccatt tcatgaaggg aaaaacaata caggtaagac aattgtattt
     2101 ggacatacga ttacaccaat gctccatgga gatatgcaga caacagacct ctggattcag
     2161 gatcataaaa tcggaatgga cggtggggct gtattcggag ggtcattgca tggggttgtt
     2221 tttgacaagg aaggtattct tcaagatatt gaatatcaga acaacgaagg accttggcat
     2281 ggaatctgac taatggaaag aatgaaggag ctgtgacttt gttgcggctc tttttatttt
     2341 tatagagaat attgtttctt tcagtagttt aacccaggat aaaaagtatg atagaataaa
     2401 gaagcaattt ttagcagaaa agaggcattt taatgacgga aaatttgaac cagacgatca
     2461 ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa gcagttgact actgtactta
     2521 atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg ttaccgaaaa gaaatgactg
     2581 gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg gtatgcctat cttgaaaact
     2641 tagaaaaacg taaaactgaa gtgattcggc taatcgatga acaagggaaa ctgactcccg
     2701 agttagaagc agaaatcacc caagccgtga aaatgcaaca agtggaagat ttgtatcgtc
     2761 catacaaaca aaaacgacgg acaaaagcaa cgattgctaa agaaaaagga cttgaaccac
     2821 tcgcgaagtg gctgatgcag cttacagatg gagaggtgca atctgaagca gaaaaatata
     2881 ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt gcacggtgct catgagatta
     2941 ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg gattcggtct tatacgtata
     3001 acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc agatgaaaaa ggcgtatatg
     3061 aaatgtatta tgactttgca gaacccgtcc ataaaatggt ttctcatcga atattagcta
     3121 ctaatcgtgg agaaaaagaa gatattctga aagtattctt tcaagtagat gaagcagcaa
     3181 ttttagctta cttggatcgc caaatagtca aaaatcctgc ctctccttct tcttcattcg
     3241 tcagagaagc ctatcaggat agttataaac gctttattca gccagcaatc gagcgagaac
     3301 tgcgcaatga attgactgaa aaggcagatg agcaagcaat cgctattttc ggcgaaaacc
     3361 tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt tgtcctggga tttgatccgg
     3421 cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac agggaaagtt ttagcaatcg
     3481 aagttattta tcctcataaa cctgcagcgc aagcaaaaag agaagctgct ggaccagctt
     3541 ttatccaatt aattaataaa tatcaagtag atatggtagc tatcggaaat ggtacagcca
     3601 gtcgggaatc tgaacttttt gtggctgagc agttgaaatc cgccgaccat aaaacctatt
     3661 acgctatcgt caacgaagca ggcgcttctg tttactccgc tagcgaaatt gcacgaaaag
     3721 aatttccgca tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt cgcctgcaag
     3781 atcctttagc tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc cagtaccaac
     3841 atgacgtttc tcaaaaacgt ttggctgaac aattggattt tgttgtggaa acagcggtta
     3901 accaagtagg tgtggatgtg aatacagcaa gcccgcagct cttgcagcat atatcaggat
     3961 tgaataaaac aactgcccaa aacattgtca tttatcgtga agaaaacgga gaattcaccg
     4021 cacgaactca attgaaaaaa gtgccacgct taggaccaaa agcatatgaa caagcaatcg
     4081 gattcttgcg agtccctggt gggaaaaaca ttttggacaa cacaggtatc catcccgaaa
     4141 gttattctat tgcaaaagaa atattaactt cagtccaact ttcagaaaaa gagcttggta
     4201 cagaagaagc agtagaaaaa ttgacacgat tatccgtaga gaaactggct gaaagcctat
     4261 cagtcggaga agaaacgtta gccgatattt tagctgggct gacacaacct ggacgagata
     4321 tgcgtgatga gatgccagcg cccttattac gtacagatgt attgagtatg gaagacttga
     4381 aaccagggat ggaattgact ggaacggtcc gtaatgtcat tgacttcggc gcatttgttg
     4441 atatcggtgt gaagcaagac ggacttgtcc atatatcgaa actcagcaaa aaattcgtca
     4501 agcatcctac agatgtcgtg tcagtaggag atatcgtaac agtttggata gaacaagtcg
     4561 atacgaaaaa aggcagaatc agtttgacga tgctttcccc ttatgaagag taacaatgag
     4621 gagtaacaaa aattgacaca agaagaactg cagcagttag tagaagagat atcattaaaa
     4681 agcttccagc ttcctttcaa gcaccaagca atgttcaatc cacgcttacg tacaactggc
     4741 gggcgctatc atttaaacga tcatcattta gattttaatg cgaagctatt tgaagagtca
     4801 gacgaagaga cgattgcagg aatcatcaaa catgaacttt gccattatca tcttcatcta
     4861 gctggaagag ggtatcagca taaagatatg gatttcaagt atcttttgaa aaaaacagga
     4921 gggttgcgct atgcaccagc cttgacgaag aaacagataa agatggagta ttatcgatgc
     4981 caagattgtt ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa atatcgttgt
     5041 ggaagatgtc atggagaaat caaatggatt gagacaaaaa gagaagactt taatgaagca
     5101 caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag gaatctaaaa
     5161 gactggaatc agctattaaa ttttttagcc gcacctgaag agggaaattt tgaggaactg
     5221 acggcaccta cacttatttt agctggaaac tgtcttccta tcttagctga taaagcagca
     5281 caaatgtatt taaatggcca agtagatcaa ttgtttttgg tcggaggggt ggggcatgcg
     5341 actagaatcc tttatgagaa ttttgaaaag caaggatttc attttgaaga tggcatgagt
     5401 gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg atttgcccga taaggctttt
     5461 ctgatagaat cgaagtctac aaattctgga gagaacgcca ctttttcact ggagattctt
     5521 cattccttag gagctgtacc agaaaaaatc ttattgatga atgatccgac actgcaacgc
     5581 aggactcgag caacttttga gaaagtatgg caaaatgaac aaactatttt cgcgaatgct
     5641 gtgccatttg tgccggaaat ccttcacttt tctgaagaga tcatctttac agcaaaagaa
     5701 ctgaatcatc aatggcctaa ggaatatttc catgcgctag tattaggaga aatggaacgt
     5761 ctccatgacg atgagaacgg ctatggtccg aaaggaaaag attttattcc tcacattgat
     5821 atatcagagg aagtttggtc ttcttacacc agaattaaag agtcaataaa aacggatttt
     5881 tctcgtactt gaccatttta gcaagcgaat cctcctttcc aaataaattt ggttgaaatt
     5941 ttcaatcgtt tataagataa tgaggagcgt gttgaatgta atagctaaaa gaatggacgc
     6001 tagataagag aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt ttgttggtat
     6061 caaaggttca ggaatgagct ccttggctct tgttttacat gaacaaggtc taaatgtcca
     6121 aggatcagac attgaaaaat atttctttac acaaagagat ttagaaaaag caaatatcac
     6181 gattctgcca tttaacgcag ataatgtaaa accagggatg acgatcattg caggaaatgc
     6241 atttcctgat tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg aagtgattcg
     6301 ctatcacgac tttattggtc attttatcca aaattatacg agtatcgctg taacaggctc
     6361 tcacggaaaa acaagtacga ctggactgct ctctcatgta ttgagtggtg tccgtccaac
     6421 aagttatcta attggagatg gaacaggaca tggtgatccg caagcggaat tcttttcatt
     6481 tgaggcctgt gaatatcgcc gtcatttcct tgcctattca ccagactatg caatcatgac
     6541 caatatcgat tttgatcatc cggattatta tacaagtatc gacgatgtat ttacagcttt
     6601 ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg tatggtgacg atgcttattt
     6661 acgcaaatta aaagcaaatg tgccgattta ttattatggt gtcacagaaa acgatgatat
     6721 ccaagcacga aacatcgaac gcacaacttc tggttctgct tttgatgtct atcatgggga
     6781 tgaatttgtt gggcacttca ctgtaccagc attcggtaaa cacaatatct taaatgcttt
     6841 aggtgttatt gcagtagctt atttcgaaaa actggatctg aaagaagttg cagaagaaat
     6901 gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag acatgaccgt
     6961 agtcgatgat tatgcgcatc accctgcaga gatcaaagca acgattgatg gcgcacgcca
     7021 aaaatatcca gataaagaaa taattgcagt tttccaaccc catacgttta cgcgtacgat
     7081 tgcattgatg gatgaatttg cagaagcact tgatttagct gataaagttt acttatgcga
     7141 tatttttggt tcggctcggg aagaacaagg aaacgttaag attgaagacc ttggagcgaa
     7201 aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta tctccattgt tagattatca
     7261 tgatgcagta gtaatcttca tgggtgctgg agatgttcaa aaatttgaac aagcgtatga
     7321 aaaactttta agcagcacga cgaaaaatgt actgtaatac aattactgca gtgtccttat
     7381 aaaaatagaa agagggggag tggcaaaaat cttgccaagc cccctttttt gcaacttatg
     7441 ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caatcaaatc aaaaacatga
     7501 gaagctgttt tttcttggct gttcttatta tttgatgcag gacagctttt tcacgacctc
     7561 tgtaaactat aggaatctgt taaatcaagg gagcagaact tgccttttac gctacttttg
     7621 gtacaataat gtatgattaa aaataaactg gagggtttct atcttgaaca taggtaagcc
     7681 gagaaaacta ggaaaaacaa taaatgaaat cgaggaaggc gactcgttat ccttaacaga
     7741 atctattgaa gataaccagc ttttgctgta tttgggattg acgaatgatg caaatccttt
     7801 gtatatccaa catgactttg cacaaaaaac agaatatcaa cagccaatcg ttccatcagt
     7861 gatgctgatg gggatcatca caagtgcaat ttcaaagcat cttcctggac caggatcaca
     7921 tgttgtcaat ttttctgtca attttattga accggttttc cattatgaaa cactgacttt
     7981 tcagtttgag gtcataaaag tagataagat gaaggatgtg gtgacgattt ctgtagaagc
     8041 ggagaatacg aacgaagatc gtgtgttgga tgctgtcgtt atggtccaac cgccacaagt
     8101 tcctgaattg gaaatagcaa tcgaaagtga aggtgaattg aatgaataat ccaagtacat
     8161 caatgtcagg tatcaatcgt ttttatgcaa aagtttatgg atttttaggt atgggacttg
     8221 cccttagtgc agtcacttct tacttggctc tttgggtatt tcctttgcaa ttagcaactt
     8281 ttatcaataa ttttccacta ggctttgtgg gtctgtggat cgttgaactg attttagtaa
     8341 tggttctagg cgtaaaagca caaaagaatc cgagcttagc aatcggtgga tttattgcct
     8401 actctctttt gaatggcata acattagcgg ttactcttgc atactacgat attggaacag
     8461 taacgcaagc tttcttgaca gctactttga tgttcatggg gatgtctgtt gtcggagtaa
     8521 caacaaaaag agatcttagc ggggttggaa gagcaggaca ggcagcttta atcggagtga
     8581 tcattgcact agtcttgaac atcttcattt tgagaagtag cgtagttgag ctgttcatct
     8641 ccctactgtt agtcgtcatc tttgcaggga ttacagctta tgataaccaa agcatccgta
     8701 atatctatgt ccagacaaac ggaacagcac atagtggaat tgctgtattc atggcattga
     8761 agctgtattt ggactttatc aacttgttct tggcgtttct gagaatcttt ggacggaaca
     8821 actaaaaaag aaaaaaattc atcagttggg gctataccga attatttggt atggtcccgc
     8881 tttttctttt cgcattccgc aggattcttt gttagaatga ataagaactt tacagtaagg
     8941 agttaagaag atgacaaaaa ataaattatt actcgttgat gggaacagtg tggcttttcg
     9001 cgcattcttt gcgcttcaca attctcttga acgtttcaaa aatagaaacg gtcttcatac
     9061 aaatgcaatc tatgcattca atacgatgtt tgaaaatgtt atgcaaaaag agcaaccgac
     9121 tcatgttctg gttgcgttcg atgcaggaaa aacgactttt cggacagaaa tgtatgcgga
     9181 gtacaaaggt ggaagatcca aaacacctgg cgaattcaaa gaacaaatgc catatatacg
     9241 tgatttgttg acaggactag gggtacagta ttacgaattg cccaattatg aagcagatga
     9301 tattatcggt actttggcag aaaaagtaga caaagatcaa tttgatgtcg tcgtgctttc
     9361 aggtgatcgg gatttgactc aattagctag taaagaagta aaagtggata tcactgtaaa
     9421 aggtgtcagc gatatcgaaa gctatacacc agaacatgta gcggaaaaat atgatgggtt
     9481 gacgccaaaa cagattattg acatgaaggg actagccgga gatgcctctg acaatatccc
     9541 cggtgtgact aaaatcggtg aaaaaacagc tatcaagcta ttgaaacaat atggctcagt
     9601 cgaaggaatt tatgaacata tcgatgaaat gaagcaaagc aaaatgaaag aaaatctgat
     9661 aaatgacaaa gagcaggctt tcttatcgaa aaaattggcg accatcaata cttcagcgcc
     9721 tgtggatgtc tctatcgact cattgaagta tgaagggaaa aatctcgata aactcgttcc
     9781 gttttataaa gaaatggatt tcaatcagtt cctttctaaa ttgaatatcg tagaagaaga
     9841 agtaaaaatg gatgatattt tgtttgaagt cgttcatgaa ttcaaagagg aaatgtttac
     9901 tacggatatg gcgttgtacg tggaaatgat gggcgacaat tatcatacag aggaaatcgt
     9961 cggagtagcg tgggggacag aaaagaaaat ttatgtcacg aacgatctat ctgtattcga
    10021 aaaccgagcg ttccattctt ggatcactga tccgactcgg ctgaaaaaag tatatgatgc
    10081 gaaacgaact tatgttgctt taaaccgcta tactggaaaa acgaaaggaa tcgattttga
    10141 tgtcttgcta ggtgcatacc ttctcgatac gaatgaaaaa agcacagata tcgaaggaat
    10201 agctgctcat tacggataca acgatatcca atcagatgaa gctgtatacg gcaaaggggc
    10261 gaaaaaaggt ttaccagaag aagaggaact cttttttgct catttagcgc gaaaagtagc
    10321 tgcaatcaat gcgttgacac caaaattgag ccaagaactg gcagaaaaaa accaagaaga
    10381 tcttttccgg aaaatggaac tgccattgtc tatcattttg gcagagatgg aaataagcgg
    10441 gatcaaagta gatgcgactc gcttacaaga aatgaagggc gagttctctg ctcgcttaag
    10501 agagatcgaa caaaaaatct acgaagaagc cggcgaagaa ttcaatttga attcaccaaa
    10561 gcaattagga gtgatcttat ttgaaaaaat gggattaccc gttatcaaaa aaacaaaaac
    10621 tggatattcc acagccgtag atgtactgga acagcttcgt gaacaagcac caatcgtaga
    10681 ggatatcctc acctatcgtc aaattgcaaa aattcaatct acctatgtcg agggactgct
    10741 gaaagtgatc caatcagatg gaaaagtgca tacccgttat gtccagacat tgacccaaac
    10801 aggacgatta agctctgttg atcctaattt gcagaacatc ccgattcgat tagatgaagg
    10861 gagaaagata cgtcaggcat ttgttcctag agaaaaagac tggctgattt attcttctga
    10921 ctattcacaa atcgagttac gcgtacttgc gcatatctca aatgatgaac atttgaaagc
    10981 tgccttccta gaaggtcaag atatccatgc cagcacagcg atgcgtgtat ttggaatcga
    11041 aaaggcagag gatgtcacac caaatatgcg taggcaggca aaagcagtga attttggtat
    11101 cgtgtatggc atctctgatt acggactttc acaaaatcta ggaatcagcc gaaaagcagc
    11161 acagcaatac atcgatacgt actttgaaaa atacccaggt gtcaaagaat atatggaaag
    11221 aatcgtacgc gaagcaaaag accaaggcta cgtcgaaacg ctgtatcacc gccgtcgata
    11281 tctgccagac atcaattcaa gaaattacaa tatccgttct tttgcggaac gaacagctat
    11341 caatacacca atccaaggaa gtgctgctga tattttgaaa atcgcgatga tcgaattgga
    11401 taaacgatta aaagaaacag gtcttcaagc tacgatgctg ttacaagttc atgatgaact
    11461 tgtcttcgag gtaccggaaa aagaacttga atcattagat aaattggtga aggaagttat
    11521 ggaacaagct gtttctcttc atgtaccact gatcactgac agcagttggg gaaaaacgtg
    11581 gtacgaagca aaataaacat tgtttaagac agagcaatat aatgaaggtg aaaagaaaaa
    11641 atgccagaat taccagaagt agaaaccgtt cgtaaaggat tggaacggtt agtagtagga
    11701 aaaacgattc aaaaagttca ggtcttgtgg cccaagatca tcgagcaacc agagacaccc
    11761 atctttgagg cttctcttgt tggcgaaacc atccaatcca ttggacgtag agggaagttt
    11821 ttaatttttc atctcgatca ttgtgaactg atctcacact tgcgaatgga aggaaaatat
    11881 caatttacga aagaaaatac accaatcgat aaacatacgc atgtactttt cttttttgag
    11941 gatggaagcc aacttcgcta caacgatgta cgaaaatttg gtcgtatgac aatcgtcgaa
    12001 aaaggagcaa gtgcaactta ccgaggaatc atgaaattgg gacctgagcc tttgccagat
    12061 agcttcttat tagcagattt cgcaaatgga ctgaaaaagt cccacaaagc aatcaagcca
    12121 ttgctgctag accaacgtct agtgactggt ctaggtaata tttatgtaga tgaagcatta
    12181 tgggaagcga agatccatcc agaacaaccg gcgaacacac tgcgccccaa agaggtagaa
    12241 caattacgtc tatcgatcat tgatgttttg gatcgtgcaa tagaagccgg aggtaccacg
    12301 attcgcagtt atttgaatgc attaggagaa tcaggcggat ttcaagtagc actgcatgtc
    12361 tatcagcaaa ctggcaaacc atgtatacgt tgtggaacgc cgatcgtgaa aacaaaagtg
    12421 gcacaaaggg ggacgcacta ttgtccgaat tgtcaaaggc taaaaatcgc aaagtaggat
    12481 ttgttttagg tttgacgggt ggaatagcta ccggtaaaag tacggcagcc aaagtctttc
    12541 agtctcatgg atttcctttg atcgatggcg atgtgattgc cagagaaact gttgagcctg
    12601 gaacgcttgc tctgaggaaa attgtatcag tgtttggcca agaggtattg cagcctgatg
    12661 ggcaactaga tcgagggaaa cttgggatga ttgtttttcc atcaaaggaa aaacggcaaa
    12721 aacttgatca gctattagat ccgtttatcc gaaaagcgat aaaagatcaa atcgcctctc
    12781 taagttccaa tcatccttta gtaattgtag atatcccatt gctctacgaa ggtcattatg
    12841 accactatat ggatgcagta gctgtcgttt atactacgcc agaaactcag cttcagcgtt
    12901 tgatgaaacg taatcaactg acgctagaac aagcgcagca gagaattagt agtcaacttc
    12961 caatcgaaga aaaaaagcag cgtgcggata ttttgttcga caacaatgga acaaaagaaa
    13021 aacttgtggg acaaatcgaa aattggcttt ttgctaatcg ctttctttga gtatctgtgt
    13081 caattttgct atcctgagta tatagaaagt gagggatagc gatgggatca ttaaaaaatc
    13141 aaaaggataa agtcgtaggt aacgcaaaag aaaaaatcgg cgagatgctc aataaagaaa
    13201 atctaatcga tgaaggtcgt acgcaatcag aaacagcaga agaaaaagaa aagcagtacc
    13261 aagcaagaat cgatgaaata gaagaccagc aggcagaaga agaagcgaag cggcaagatt
    13321 tgactcaaga acgaaaaaat ccgacacctg atcgtagtaa aacaccgctc ccagaaagtg
    13381 accacattcc cgatgagcaa aaaatcgagg aacagcgaat gaaacactat acaggaatcg
    13441 aagagaagtt ataaaatagt aaaaaatcga gatgtgactt gaagcagatg agaaggtgtt
    13501 catctgcttc aagttttttg tgctagatga ctaaatcagc ttttgtgtaa gttatttctc
    13561 agtaatcaag gtgatcagta ttggaaaatt gaaatttagt aaggacaggg gacacaatta
    13621 cgcatgaaag tagaaaaaat tgttcttata tccgtgtttt ttgggtagga cagtcgcgtt
    13681 tgtgatttgt ttcgtgttat aatacaacta gttgtaaaga aagacacatc ctttatataa
    13741 aatgcgatgc aagaggtgag acaaaatgag atgtcccaga tgtcaacata ataattcacg
    13801 agtaatcgac agccgtcaag cagatgatgg acgtgcgatc cgtcgccgtc gcgaatgtga
    13861 gaattgttcg ttccgattca ctacatttga acgaattgaa gctgctccgt tattagtaat
    13921 caagaaaaac ggggaacgcg aagagttcaa tcgcgataaa attttaagag gactcatccg
    13981 ttcagcagaa aaaagacctg tagctatgga acaaatggaa caaatcgtca accatgtcga
    14041 aaaccgcgta cgcagtctcg gagaaaacga agtatcgacg aatctaattg gtgaatatgt
    14101 gatggaagat ttagttgatt tagacgaaat cgcttatatt cgttttgcaa gtgtttatcg
    14161 ccagttcaaa gatatgtctg ttttcttaaa agagttgcaa gatatcgtgg ataaagcaaa
    14221 agaaaataat aaagagaata ataaagaaaa caattaagaa acaaaaagga ggcatttcct
    14281 tggaaaatgc ttggaaagag cttcaaccaa aaaatattta ccaaggaaga aaagcggctc
    14341 cgctgagaga agacagcaag aacgccttgc tgtatcttta tcagccacta atcggcggag
    14401 aagccttgag tatctatttc accctgttat cagaaatctc tcaagaatct ggcaaaggtc
    14461 ctgaagggat gcatgcagat ctgcttagta gtctaggctg cggtatcccg caattttatg
    14521 atgcaagaaa aaagctagaa ggaatcggct tactggatgt ttattttaaa gaagatagtg
    14581 atctgggacc tcgttttatt tatgagctgt tagaaccact cattcctaaa gcatttttca
    14641 aagactctgt tctggctttt cttcttctgg aaaaagttgg tgaacgacga ttccagaagc
    14701 ttacccaatt ctttgaacct aaaaggcata cagcagaggg atatcaaaaa atcacaaaaa
    14761 aatttttaga tgtttatcgc ttcaatgaag cagtgtacgc tgctaatcaa gaaaacatcg
    14821 aatcgctgaa taaatctttt gaagaatctg aatcagtagc attacttgaa gcagattcga
    14881 cattcgattg gcagtttttg aatgattggc tggcaaaaaa acatattgca agtcttgatg
    14941 atccaatcat caaacaagtc gacatgtatc atcagcttta cggtttcgat gaattgacac
    15001 ttggcgaact gatcagtcaa tcttatgact ttagtgaaca aaaagtatct gaaaaagaac
    15061 tgcaaaaagc cattttggca atgcagagac gtccggtgaa gcaaacaaaa gaaacagttg
    15121 cggtttctga aaacagtaat gaaccctcgt caaacgaaca tttgacaaac gcagcgcttc
    15181 agctgataaa agaagcacag agcattccac cgatgaaata tctggaagca atcaagtcag
    15241 aaaaaggcgg atatgtttct aaacaagaaa actggttact gcaagatctt gtttctcaat
    15301 caggtctttc cagtagcgtg atcaatgttt tgctgaatta tgtactagtg atcaagaata
    15361 atgctagctt aaatgccagt tatgtaaata cagtggcaaa tgaatgggca caaaagaaaa
    15421 tcgccacagc agaagaagcg atccagcaca ttcggcaaat aagtaatcaa gcaaaacaat
    15481 caaaacaaaa gaagcaaaca aattatcaaa ctggcaagcg aaatgttcga cgggaaaagc
    15541 ttcctgattg ggtcaaccag ccaaaagatg aaaaacagat cagcagcgag aaaaaagcag
    15601 agattgaccg ccgttttaaa gaatacctgg caaaaaagga gggtgatatc tagtggaaga
    15661 cgtaggcaaa gaattgaaga ggatcatcag caaacgcaat tatcaagaaa agtatgctga
    15721 aatgatcgct gaagtcttaa atgatgcgga tgttcaagct tttctaagcg cacaccaaga
    15781 agaattgaca caagcggata ttgaaaaaag ttacgctaaa ttgtatgaat tcgtgcaaga
    15841 aaagcgtaaa tatcaaaaaa acgatccaac gatgatcgct cccggctatg agccgaagct
    15901 gacgttgaat tttcattatg tggatgtcac ttatgtacca acggaagcat tgttagcacg
    15961 gcaaagacaa gaagaaatca gaagccgagt aaaagcaatg gatatgccaa aagatattca
    16021 atcggcttct tttgaaaact ttgaacgtac agatggacga ggatatgcga gcttagaagc
    16081 cctagatttt gtagagaaat atactgctga tccaaaaggg tatcataaag gactgtatct
    16141 agtaggaagt tttggtattg gaaaaacata tttattagga gcaacagcga gagaattagc
    16201 gattgctgga ttcaccacga cgctagtcca ttttccgaca tttgccgttg agatgaaaca
    16261 ggcaatcgga aaagatcaag tggcagaaaa gttagatgca gtgaaacgtt cacctatttt
    16321 gatgatcgat gacattggtg cagatgcgat gagtagttgg atacgagacg aagtattcgg
    16381 tgttatcctt caatatcgga tgcaggaaca gttaccaacc ttcttctcat cgaattttac
    16441 aatggatgaa ttggaacagc atttgtccgt cactcaacgt ggagatgaag agccgctaaa
    16501 agcgaaacgg ataatggaac gcatacgtta tttgacaaaa gaaatcgaaa tgactggtcg
    16561 caaccgacgc aatggttgaa cagttgacaa gtccaactga catttgacct agtagtaaaa
    16621 gcaggtcagc gaatcattat atggacagaa atgaccggag acaatcaccg caacaagtaa
    16681 tgcagaaact taaacggagg attttttatg agggctattt tttctagtat atctgtagtt
    16741 ggtctatttt ttcttttagc aggctgcggt agcgcagata gcaacacagg ttctgttgat
    16801 tcgattgatg cgggtcaaac agtaaaaaca agcagtacga aaacggagag acaaagcata
    16861 tttgtcggaa cgattgaaag tatcactcaa gtagaagcag aagaagaaac gcttcagttg
    16921 aaactcgtcg atgtcatggc caaagaagat ccagcgaata tgggaacgtc attcagcaat
    16981 gacggagtga tcttaaatgc tactgctgaa aaaataagcg tggacataaa tgaattgaaa
    17041 atcggtgaca aagtccaatt tacattagtt gagatgccga tcatgacgat gtctatccca
    17101 ccgcaagttc ctggagattc gattattgat gtttcattga acaaataacc aataacattt
    17161 tttcaaaaat aagaaaatgt tgttttatag aacgaaaagg tatatttttt aaggagatat
    17221 gggaattttt ttattcctag tctccttatt ttatttggaa aatgctataa ttatctagaa
    17281 ggagtgaagc tatgaaccat ttagatttat ctcagcgctt agccgcagtg ggtgactttg
    17341 tccctgaatc cgctcgttta gcagacattg gatcagatca cgcttactta cccgttgcat
    17401 tgatattaag aggaaaaatc gaatatgcag ttgctggtga agtcgttcaa ggtccttatg
    17461 aatcagcacg taagcaagtc aaaaaagatg gattagaaga ccgtatcacc gtacgccttg
    17521 caaatggtct agatgctatc cagccacaag atggtattac agctgtcacg attgcgggta
    17581 tgggaggttc actgatccga gatatcctag aagctggcaa gttgaatcaa cgcttaagcg
    17641 gacaagaacg tttgatcctt cagccaaaca tcggagagcg tacattacga tcttggctgc
    17701 aagaaaatca gtataaaatc caatcagaaa aaatacttga agaaaataaa aaaatatatg
    17761 aaatcattgt agccgaaaaa gaacaaaaac cagttactta caatgaaatg gagctgatgt
    17821 tcgggccatt tctgcttgaa gagaaaagtg agatcttcaa atcaaaatgg acaagagagc
    17881 ttcaacaaag aaaagctatc cgtgaacagc tcaaacaagc agctaatcaa gaggcacgtc
    17941 aaaaacaaat cgagcaggaa atccaaatga ttgaggaggt tttaaagcca tgacaatcag
    18001 cggacgcact ttcatccaaa agtttgaaag ctattgtcct cagtggctag ctgaagaagg
    18061 agacccagtc ggactgcata tcggaacatt ggataagcca atccaacgag taatgatgac
    18121 attagacgtt cgtccagaag tggtcgaaga agcaatcaag aaaaaaatca atttgctgat
    18181 tgctaaacat cctccaatct ttcgtcctgt aaaacggctg gttactgatc agccacaaga
    18241 aaaaatgtat gcagatttgt tgaaacatga tattgccgtc tatgcagcac acacgaacat
    18301 ggatattatt tgggatggac tgaatgactg gttttgtgaa cttctcggta ttgaagtgga
    18361 gagttattta gtaaagactc atgagattca ttataaaaaa ctggccgttt atgtacctgt
    18421 ggatcatgca cagaaaatgc gtgaagtatt agctgcagct ggtgctggta cacaaggtga
    18481 ttataccggt acaagtttta cctcgattgg acacggtcga tttactccgg aagctggtgc
    18541 acaaccagcg attgggaaag tagggaagac agaacaagtt caagaggcaa aagttgaggt
    18601 tattttgccg gaaactatcg aaaaacaagt gattcaagcc atgcggtcag ctcatcctta
    18661 tgaggaacct gcttatgatc ttttcgctat agatgaacca gtcgaaatgt ttgggttagg
    18721 acgagtaggt gaattgccgc aagaaatttc tatagaggca tttgtagaac aagtaaaaga
    18781 agcgttccaa ctagatggac tgagaatagt ccagccaaaa aatgcaaaat cctctgtgaa
    18841 gcgcattgcg atttgcggtg gaagcggtga aaaattttat ccgcaagcaa tcgcccaaag
    18901 agcagatgtc tatatcacgg gagatatcta ttatcatacg gctcatgata tgcaaagcgc
    18961 tgggttgatt gcaattgatc ccggtcatta tattgaatcg ttatacaagc aacgatttat
    19021 cgagaaattt gaatcttgga aacaagaaga aaattgggat attaactttt tcgtttctga
    19081 aacaaataca aacccatttc aatttaatta aaaggagaga atactatgta cgaaaatctt
    19141 ttgccacgtt ttttacgata tgtcaaaaca gaaacacgct ccgatgcaac aagcaaaacg
    19201 actccatcga ctcagacaca agttgctttt gcgcaagaat tgaaaaaaga attagaagag
    19261 ttaggcttag ctgatgtttt ctataatgaa gataacggat tcgttatggg aacattacca
    19321 agcaatgttg atcatgaagt gcgttccatt ggatttattt cccacatgga tacagcagat
    19381 tttaatgcag taggagtcaa tccacaaatc attgaaaact atgatggcga gtctacgatc
    19441 gtactagatg cggaagaacg ctatacattg aatacaaaag attttccaaa tttgaaaaat
    19501 tatgaaggcc acacattgat cacaacagat ggcagtacat tactgggagc agatgacaaa
    19561 tcagggattg cggaaatcat gactgcaatg gagatcttga tcaataatcc ttctatccct
    19621 cacggtacca tcaaagtggc ttttggtcct gatgaagaaa tcggtgtcgg agcggataaa
    19681 ttcgatgttg agcaattcga tgtagatttt gcttatacag tcgatggtgg tcctttaggt
    19741 gagttgcagt atgaaacgtt tagtgctgct caagcaaata ttacgatcca agggaaaaat
    19801 gtacatccag ggacggcaaa agatacaatg atcaatgctc tacagttagc aatcgatttt
    19861 cataatcagc ttccagccga tgaagttcca gaaaaaacgg aaggatacga aggtttcttc
    19921 catttgatgg cattggatgg gaatcctgaa gaagcttcta tgagttatat cattcgcgat
    19981 catgatcgtg aaaaattcga agcacgcaaa gcacaaatca cacatatcca agaagagttg
    20041 aatcagcgat ttgatcaaga acgaatcaaa gtagatatgt atgatcaata ttacaacatg
    20101 aaagaaatca ttgaaaaaga tatgagcatc gtggaattag cgaaacaagc aatgatcgag
    20161 cttggtatct ctccagtgat cgaaccagtt cgtggaggaa cagatggttc aaaaatctct
    20221 tacatgggta ttccgacgcc aaatctattt gccggaggag aaaatatgca cggacgattc
    20281 gaatttgttt ctttacaagt aatggaaaaa gcaacagacg tgattgtaaa aattgcagaa
    20341 ttgaacacaa agagttaaaa tcgaaaaaga acagcttatg acaaggcgtt cgtcccaatg
    20401 atggaatgtc atctgtctaa ggctaaaggg caggacaatg gaattggttg tcccgccctc
    20461 ttttttgaag agtaaatagg aggtaaagaa tggaaaagct gctagttttt tataacaaaa
    20521 gttcagggaa agatgagggt gagaaattag cacgctggtt tcaagattat gtaaaagaaa
    20581 agcagccaga attatatgta gaactagctg aaactggccc atcgattgat gatgcggaat
    20641 tagtcaagaa agcagaagcc atagaagctg atactttagt aattataggt ggagacggca
    20701 cgattcatca tatcgtccaa gcgttccaaa aaacattatc tcaataccat gtcggattac
    20761 ttccaggtgg aactgtcaac aaccttgcac gtgttcttga gattcccctc aaaaaagaag
    20821 aagctgctga cgtgatcctg gaaggcaatg tacggaaaat agactatggt caagtcaatg
    20881 atgaagtcat tatctctaca ctgacaatcg gaatcttagc agatacagca gtttggatca
    20941 gtcaagaaga aaaacagcgt tacggctcgt ggatctttat caagaagttt tttaagttat
    21001 tgctgaagaa gaaaaaatat catttggatt tagaaacaga cgaggagcat tggaaaggaa
    21061 aatcccaact gttgacagtc acgatgtcta actccgtggg cggttttaca aattttgacg
    21121 actcagcgac cctagatgat ggaaaatttc atgtaacgat cgtaccttcc ttagatattt
    21181 tccgatttat tttttatttg cctagaatca tgagaggcaa attctatacg atccccggca
    21241 ttacttattt cacagccaaa cgcatgaaaa tccaagcgca ggaaaaatct gtgggcactc
    21301 gaacggatgg agacacgaca gatgatcttc cagttgaatt atctgttgta tcaaaaggat
    21361 taacagttta ttcaccaaag aaagaagaaa aagcttagaa atagtagtta acagaaagtt
    21421 ggaagaagag taatggatgt attgttacac aatcctttgt ggttttacct tttcattgtg
    21481 aatttttact tattttgtct gatggggtat gataaacatc aggctagaac tagcggctgg
    21541 cgtgttcctg agaataattt gatatttgtc agcctgttag gcggtggttt gggaggattg
    21601 atctctcgaa aagttttcca tcataaaact cggaaaaaga agtttactat aggattcgct
    21661 gcaggtgtac ttgttgattt gattctcatt tattttttcc attaaggagc aggagatgtc
    21721 gtctaaagga acgaaaacta aaatcattct gaatctgctt ttgctgattg tgatttttgc
    21781 cattatttat tatttaatca atcaatcatt tgcagatatc tttgccgaat tgctgtctac
    21841 cagtgttgtt gttttggcag cggctgttat attagggaca gtttaccaaa tcgctgaagg
    21901 gcgctcaatc aaagaaattg ctgaaccttt cgcgccgaat tttacaacga aagatgggtt
    21961 cttgacctct agctatattg cgttttaccg aatcgtttcg tttggtacag gaacgctgat
    22021 ctcggagatt tatttttata acaaaaaagg aatgaagcta tcccagggag caggggtgac
    22081 agcactccat atgattatgt acaaactggc agtgatcact tatgcattag ctggcttggt
    22141 catccaattc tctctttttt attctaatgg tccatcaatg atctggttta tcatcgctgg
    22201 tatccttttg acgatcgtga ttgttgcctt tttattggcg atgtccgtca gcttgaatgt
    22261 tcaaatattt tttgtcagct tatcgaataa gttatttaaa agtgagcgtt tgcggaaatg
    22321 ggtggataca tgcaatcagc agatctactc tttaagggaa acggtccaaa ccattatcca
    22381 agacaggact gcgctattgc ggatttatct atggaatatg ttaaaacttg tattttggta
    22441 tgtacttcct tatttgattc tgattgaaaa ccatcctaaa atagattttc tactgaccat
    22501 ttcgtttatc agctttgcag tagtgctttc aggggttatt cctacgcctg ctggtattgg
    22561 ttcctttgaa tttgtctatt tgttattatt cagaccagtt gttggaacag ttgacgcagt
    22621 ttcttcgctt ttactgtatc gtttcagttc ctttatcttg ccatttctct atggatttgt
    22681 gtatgtgatt gctgaacggc gaagagtgat caaagaagaa ataatagaag taaaaaaaga
    22741 gaataaagaa aatcaagggt aggtctttta taagatctac cctttttatc aaatcaaaga
    22801 aaaacgttat ggataagata attttacgtt tgtttggctc tttttttata tacatacaat
    22861 caattttgtt tttttaagat ttatataagt cgagaaaaaa agtcaaaaaa ctatgtgtat
    22921 attttgacca aaattgacca aagcgatata atagtttttg taaagatcaa agaaataagt
    22981 gatcttacct ttaattagga gggtatacct gtatgaatat tgagaaaatg actacgacgc
    23041 ttcaagaagc gattgctgaa gctcaacaga ttgcggtcac tcgaaaacat caagatatcg
    23101 acattgccca tgtatggaaa atctttttac aatctaatca ttttggtcga aatttctata
    23161 cggatgccgg attggatgtc gaatcttttg aacatgaaat tgatcgtcta ctggatgagt
    23221 atccagttgt ttctggtgga aacgtccaat atggacaaaa cctcagccag aatttctttc
    23281 gattattgaa tgaagcagac caaatacgtg aaagcttcgg cgacgagttt ctttcaacgg
    23341 aagtcgttat tttagcgttg atgaaattaa aaaattatcc gttaactgtt tatttgaata
    23401 aaaacggatt atctgaaaaa gaattgcgta aaaacatcga agagatgaga ggaggagaaa
    23461 gagtgacctc acaaaaccaa gaagaacaat acaaagcatt agaaaaatac ggcgttgatt
    23521 tagtacagca agtcaaaagc ggaaaaatgg atccaatcat cggtcgtgat gaagaaatcc
    23581 gtgatgtcat ccgtattctt tcacgaaaaa caaaaaataa tcctgttctg ataggagaac
    23641 ccggtgtagg gaaaacggca atcgttgaag ggcttgctca acgtatcgta cgcaaagatg
    23701 tgcctgaaaa tttaaaagat aaaacgattt tttcattaga tatgggtgca ttgatcgctg
    23761 gagcaaaatt ccgtggtgaa tttgaagaaa gactaaaagc agtcttgaaa gaagtcaaaa
    23821 aaagtgatgg acgtatcctc ttgttcatcg atgaaatcca caatatcgta ggggcaggaa
    23881 aaacagaagg aagtatggat gccggaaatc tgttgaaacc aatgcttgct cgcggtgaat
    23941 tacatttgat cggtgccaca actttagatg agtaccgtca atatatggaa aaagacaaag
    24001 cgttggaacg tcgctttcaa aaggtattag tcaaagaacc aacagtggaa gatacgatca
    24061 gtattctgcg tggattaaaa gaacgttttg agatccacca tggtgttaac atccatgaca
    24121 acgcattagt agcagcagct actttatctg accgttatat tactgatcgg ttcttaccgg
    24181 acaaagcaat cgatttgatc gatgaagcca gtgcaacgat tcgggtagaa atgaactcta
    24241 tgccaacgga attagatcaa gttactagac gtttgatgca gttggaaatc gaagaagctg
    24301 cactgaaaaa agaatcagat gatgcaagta aaaaacgctt gaaaaatttg caagaagaat
    24361 tagctgaact tcgtgaagaa gcaaatgcga tgaagatgca gtgggaaaca gaaaaagaag
    24421 aagtcaattc tgtttcagca aaacgtgctg aaatcgataa agcaaaacac gaattagaag
    24481 atgcagaaaa taactatgat ttagaacgcg ctgctgtatt gcgtcatggt acgatacctc
    24541 aattagaaaa agaattgaag gaattagaag ctaaagcaaa agacagcgag attaagatgg
    24601 ttcaagagtc cgttaccgaa aatgagattg ctcaagtcgt tggcagatta acgggtatcc
    24661 ccgtaacgaa acttgtagaa ggtgaacgag aaaaactaat taaattgaat gaaacacttc
    24721 ataaacgagt gattggtcaa gatgaagcag ttgatgcggt cagtgacgcg gtaattcgtt
    24781 cacgggctgg attgcaagac ccaaatcgtc ctctaggttc tttccttttc ctaggaccaa
    24841 ctggtgtcgg aaaaaccgaa ttagcgaaag cgttagctga gaacttgttt gactcagaag
    24901 atcatatggt acggatcgat atgagtgaat atatggaaaa acatgctgtg tctcgtctag
    24961 tcggagctcc tccaggctat gtaggttacg aagaaggtgg tcaattaacg gaagctgtca
    25021 gacgaaatcc ttatacaatc gtcttgttgg atgaaatcga aaaagcgcat cccgatgtct
    25081 tcaatatctt gttacaagtc ctagatgacg gacgcttgac tgattcaaaa gggcgagtag
    25141 tagatttcaa aaatacggtt ctgatcatga ccagcaatat cggttcacag ttactgctgg
    25201 aaggcgtgac tgctgatgga acgattccag aagcagtggc tgaacaagtc aacacgctat
    25261 tacgtggaaa cttcaaacca gaattcttga accgaatcga cgacacgatt ttatttacac
    25321 cattgagcct agacaacgtg aaaggaatcg ttgacaaaat ggtggcgcaa ttggcacaac
    25381 gtctggaaca tcaagaaatc ctgctgacaa tcagtgatga agccaaaacg tggattgctg
    25441 aaaatgctta tgaaccagca tatggtgcaa gaccattgaa acgatttatc actaaggaag
    25501 tagaaacacc actagctaaa gaaatcgttg caggacatgt tatgcctaaa tcaaaagtaa
    25561 cgattacttt acttgatgga caacttcatt tcaaaacaga agagctagaa gaaatcgtct
    25621 aatcttccat tgagactata aaagaaaaaa ggggtctgac acaactagtg tcagacctcc
    25681 ttttttcaaa taaacagtgt tccagaagta gtttcttcga aaaataagcc gaaatttctt
    25741 gaagctgccc gcttctgtca caacctcttt tattaggtat ttttcacttt ttctaattta
    25801 taactttcaa agtaacgctc atctttttta ctttgagacc atgcaaacaa tcccattaag
    25861 gcagcaccta tcgtatttgt cagcaagtcg cccatcgtat ccatcaatgc tgctcgtccg
    25921 acaaacagtg tgccatcaga agcagcaaaa cgttgaagat tcatgcctaa gaactggtcg
    25981 cataagaatt cccagaactc ccaaaacacg ccgcaaagtc cagcaaacgc aaagccgaat
    26041 aagaggaaca accaaggact ggtttttgat atctcagcat ctttcatcaa gtaaccaatc
    26101 aatccatagc caagagctgt caggaccatt ggactcaccg catgtaagat tttgtcccaa
    26161 aaagagatga tactgatcaa atgcatccct gtgcctaaaa agacagagat gaacaagaaa
    26221 aaccagtaaa acaaaacgat tgctgctggt attttaagtt tgaaaacttt caaaatgact
    26281 tgtggcatga aaatcaatgc aatccctaat aaagattcca cgataaatgt gattccttga
    26341 gaagtcgaat atttttgtgt gataaattca taaaaattat agcagacaac gaatacacca
    26401 aaagcaagta aagctatttg cacctttttg atttctttaa tcactgaaag tccctccatc
    26461 taaaaatctt tttacttctg aaagcaactc aatttctgaa gaaaccactt ttccgttcaa
    26521 tttgatcaaa cctattgtat ataagttcag gtagtgaaat tgattttcag ctgtttcttg
    26581 tagtgcagcg atttttttcg ggtgggttcc gccttgctgt cgagtatcag tatataagcc
    26641 gatcactggg atgcctttcg cataagccac accgatttcc gatgctaccc cggcatcgat
    26701 cgtcagccca tctagtaaag caatcattaa atcactagcc aacacttttt ctgtgtcagc
    26761 aagagcaatc attcgactgt ctgcataagc agttttgtca ttgattgctt cattttcttg
    26821 aggaagatag atcgaaagct cagaagagag ctctctgatt tctttaacta actgttggtt
    26881 atataacaaa tcacttttag caaacattgg tgcggcaaaa taaattttca tctaaacatc
    26941 atccttttta tcaaaaataa tcgctaaagt aagcttccat gtttggaatg atacgctcta
    27001 gtgacttcag tgtttcttca tctgtttcta accgttcgat tcttgccaaa taagaagcac
    27061 ggcgataatt catcatcaag caagtcaatg tttggatatc cagtcgaacg gattttccta
    27121 taggttcatt taagacacgg acttgtccct gttcatccca tgttaaagcg aagacaccat
    27181 tattccaagg agcgacagga tcttcaatga tgaaatgaaa agcatcagct gttccaacaa
    27241 acggaaaccg ttgaaggaat tcttttacat caacgatccg agccataaaa tatggttcga
    27301 tctgctcttt gatctgacta tcttcaatca aaaatgctaa aggctcattt ttataaatat
    27361 ctccatgtac ccagtagatc atggaaaaat gtgcggaaat aaagttccat aagccatttc
    27421 gagcttcttg atcaagatag aacatttctt ttacgtgaaa tatttcttca gctacccagt
    27481 agaacagaac acctcttggt tcatggttgc tatcgtagta gacagcggct gtgcgctcat
    27541 cttcattttc gaaacgccaa tactcttccc aatgaaaatc actgcggaat aaagcaccat
    27601 ggttttgttg ggcaaatttg tcataaacgg aaaatacgtc tggatgatca actggtaatc
    27661 gctcaaccat accacttact gggatttgtt taggtagctg tgtatctctg attttaaagg
    27721 aaagtttatc agacatgatt tcccatcctt tacgccgata gtaggggata ctgtaaggat
    27781 acaaataaga gatccattgc ttattcttgc gcatattatc caatgcaacg ataattagat
    27841 cttgcatcaa tccatggtta gcatattctg gataagttcc aactccagtc acaccaccca
    27901 ttttataacg tgcaccgtga atattgactt cacaaggata gatagcgatt tgagagatca
    27961 gtttattttc gtggaaccat ccgaacactt tcgatacttc caaaatcggt tttttggaac
    28021 ggataaaagc tcgtttattc tcaaacccgc tttcctcgat gtctgcttca gtcacttgga
    28081 acacatagga aagcaattca ttgaattgat cgatatgttc ttgagtgacc ggttttaatt
    28141 caagattttc tctaaaatct ttttgatcca ttttttcatc tccatttatt agcttgataa
    28201 cagtatagca aaattctaca aaaaaaactt ctatagctga ctaattaaaa actctttaaa
    28261 ttactgcttt tcgttggtat aatggaaagt gctgattata tgaaaagagg agacgctatg
    28321 gatataaaga aaatgaaaga acgccaagag cagattcgta atttttcgat catcgctcat
    28381 atcgaccatg ggaaatctac attggccgat cgtattttag aaatgacgca cactgtgacg
    28441 tctcgcgaaa tgcaagatca gctgttagac tcgatggatt tggaacgtga acgtggaatc
    28501 acgattaaat taaatgctgt agaactcaat tacacagcga aaaatggaga aacatatatc
    28561 tttcacttga ttgacacccc agggcacgtc gacttcacct atgaagtctc tcgaagcctt
    28621 gctgcctgtg agggtgccgt tttagtggtc gatgcagcac aaggaatcga agcacaaaca
    28681 ttggccaatg tttatttagc gttagacaat gatttggaga tcctgccagt catcaataaa
    28741 atcgacctac ccgcagcaga tcccgaacgt gtccgtcaag aaatcgaaga cgtaatcgga
    28801 atcgatgcta gtgaagctgt attagccagt gccaaatcag ggattgggat tgaagatatt
    28861 ctcgagcaga tcgtggaata tgttcctgca ccaagtggag acttagatgc accgctaaaa
    28921 gctttgatct ttgactctat ttatgatagt taccgcggtg ttgttctaaa tgtccggatc
    28981 acggacggta tggtcaaacc cggagacaaa atcaaactga tgagtaatgg caaaacattt
    29041 gatgttacag aggtcggggt cttctcgcca aaagcagttg ctagagactt tttgatggtc
    29101 ggtgatgtcg gttatatcac agcaagtatc aaaactgtcc aagatacacg agtaggggat
    29161 acagtaacgt tagcagacaa tcctgccgct gaagctttac caggttaccg aaaaatgaat
    29221 ccgatggttt attgtggtct ttatccaatt gatacttctc gttacaacga tttgcgggaa
    29281 gctttagaaa aactgcaatt gaatgatgct gctttacaat tcgaaccaga aacctctcaa
    29341 gcattaggtt ttgggttccg ctgtggattc ctcggacttc ttcatatgga tgtcgtccaa
    29401 gaacgcttgg aacgggaatt caatctagaa ttgatcacga ctgcaccatc cgttatttac
    29461 catgttaata aaacggatgg tacgactgct acagtagata acccggcaga ttttcctgaa
    29521 cctgtaacga ttcaggatgt agaagaacca ttcgtcaaag cacagatcat ggtcccaaat
    29581 gattttgtcg gtgcagtaat ggaactttct caacgaaaac ggggagaatt cattacgatg
    29641 gattatttag atgattaccg agtaaatgtc gtctataata ttccgctttc tgaaattgtc
    29701 tttgactttt tcgacaaatt gaaatcaagc acgaaaggct atgcctcatt ggattatgaa
    29761 atgtctggtt atcaaaaaag caagttagtg aagatggaca tcttgctgaa tggagaaaaa
    29821 gtcgatgcat taagctttat cgttcaccga gactttgcct atgaacgtgg aaaagcaatc
    29881 gttgaaaaac tgaaaaaact aatcccacgc caacaatttg aggtgccaat ccaagcagct
    29941 attggacaaa aaatcgttgc ccgttcagac atcaaagcct tgcgtaaaaa cgtcttggct
    30001 aaatgttatg gtggagatgt ttctcgtaaa cgtaaactct tagagaaaca aaaagaaggg
    30061 aagaagcgga tgaaacaaat cggctccgtc gaagttccgc aagaagcctt catggccgtt
    30121 ctgaaaatgg atgagcaaga tagtgtgaaa taacttataa tcaatcgaaa aacctattaa
    30181 ataaaccttt ttgagtagat gcaacactga ggattatctg tataaaagat tttaaactgt
    30241 catttggtta tcacggcgat actaaaatta acaaaattgg aatatttttc ttgaagattt
    30301 ttagctgtca gtatatgatg aaagcatctc ataggaggtg cttttttcat ggttgataat
    30361 tcaagtggaa gggttcttaa atctctaaga aaagagaaaa aactatctca aaaaaagtta
    30421 gctgatttag caggtatttc acaaagtact ttagttaaat atgaaaaagg gtcaagaaaa
    30481 atccctaaag acgtagataa tactctaagc aagattctga atatagaaac tctaataaaa
    30541 gatgaggaag ataagattga gattctcatc ggtaaactaa ttgcatacag agatatgaat
    30601 aagttactta acaaagagtt agctgataat ataggaatta gtgaagtatt actcagttat
    30661 gtgttaaata gaaagagaaa tccgtcaaaa gaaatgcaaa aaaaaatcga tatttttcta
    30721 ttaagtaatg aaaaagaaat tttgaaggag attaatcgtg atagtgagat atttagttta
    30781 tcaaaagacg ataaaattgt tatgggaaaa agaatacgag aagtccgaaa gaaccgggaa
    30841 gagacgcttg aaaaatttgg taaaaacttt accatctata caggcaaaaa tgttataagt
    30901 agatgggaaa aaggtattaa tattccagat attgaaaaac taatgaatat tgcctatctt
    30961 ggtaaggtca ctgttcctta ccttatgtac ggagaagatt ataagaatat acttccaaaa
    31021 gatgaaagag ttagcgattt taaaaagata aattctttta gtatgggatt aaggatgagg
    31081 aaaattagga aagattatta tctagaaagg gaggagtttg gaaagctttt ttctccttca
    31141 atttcaaaat ggtccataga taggtatgag aatggtagag atatacccaa tactaatcga
    31201 attatccaat atgcttatat aggaaatctt tctttagaat ttttgattta cgggatataa
    31261 aaatagttta cgtcgatact tattaagtta tcgacgtttt ttattataga aaaaatttat
    31321 aaaaggaggt ttcttgatat gataagaaac aaaaagggtt tgttgaaagt tgctttactg
    31381 gcagcttttt tctaagtaac tttttgacaa gctcaatggc aacacaagcg gaagaagtaa
    31441 tacaagaact aactgttatg aataacagct agataaaatg ggctatgtat gttatccaac
    31501 aatttagtaa tcactagaag cctagcgaaa gttcgcttga taagttatag atgagtattg
    31561 atttgtagtg tttccaaatc aaaagggtac agagccacat gaaatatgtt gtcctctgca
    31621 gcgtcaggat gggaatccat accatggaga ttcggagatc tgtgagggaa tacttacgtg
    31681 gttaatatgt cagttgtgtt ggacgtagta atggtcatag tgggtagatt gactgaattc
    31741 ttatatgaga aaatgaaacc aacaagaaga tagggttatt tatacagaat tggggatctt
    31801 ttctggaaaa gtaatcggat aatgcgagat tcgtacggtc ttttttaaat ttctctaaaa
    31861 aaaacggcat cgaatgggtt gggtgattga taagcaatga atcatagact gatatagaat
    31921 aaaaaatgat tctatttatc tttttacatc tatcgttttt tcaagtagcc gcaaattata
    31981 tcagcattca acaatttttg tatacaagtt ctagtaaaag gatctgaaga aaaagttggt
    32041 tgttgtgaag ataaaaaaag aatcaggttt tactgagttt ttcttcaaaa tttgtgttat
    32101 taggttagga caaagataag aagaagtatg aagatttaaa aaaggaatga ggaaaagata
    32161 gtaatttccg attattggag tttttttat
//
LOCUS       DASNNU010000025        31481 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.025, whole genome shotgun sequence.
ACCESSION   DASNNU010000025 DASNNU010000000
VERSION     DASNNU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.025"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(456..1766)
                     /locus_tag="U8116_001078"
     CDS             complement(456..1766)
                     /locus_tag="U8116_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN8434087.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1778..3136)
                     /locus_tag="U8116_001079"
     CDS             complement(1778..3136)
                     /locus_tag="U8116_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN8434088.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3338..4906)
                     /locus_tag="U8116_001080"
     CDS             complement(3338..4906)
                     /locus_tag="U8116_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN8434089.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4910..5320)
                     /locus_tag="U8116_001081"
     CDS             complement(4910..5320)
                     /locus_tag="U8116_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN8434090.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5317..6240)
                     /locus_tag="U8116_001082"
     CDS             complement(5317..6240)
                     /locus_tag="U8116_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEN8434091.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6237..7115)
                     /locus_tag="U8116_001083"
     CDS             complement(6237..7115)
                     /locus_tag="U8116_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEN8434092.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7102..8439)
                     /locus_tag="U8116_001084"
     CDS             complement(7102..8439)
                     /locus_tag="U8116_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN8434093.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8442..9545)
                     /locus_tag="U8116_001085"
     CDS             complement(8442..9545)
                     /locus_tag="U8116_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN8434094.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9619..11127)
                     /locus_tag="U8116_001086"
     CDS             complement(9619..11127)
                     /locus_tag="U8116_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN8434095.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11093..11785)
                     /locus_tag="U8116_001087"
     CDS             complement(11093..11785)
                     /locus_tag="U8116_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN8434096.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11807..12781)
                     /locus_tag="U8116_001088"
     CDS             complement(11807..12781)
                     /locus_tag="U8116_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN8434097.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13081..13953
                     /locus_tag="U8116_001089"
     CDS             13081..13953
                     /locus_tag="U8116_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN8434098.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14411..16609)
                     /locus_tag="U8116_001090"
     CDS             complement(14411..16609)
                     /locus_tag="U8116_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8434099.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16841..17833
                     /locus_tag="U8116_001091"
     CDS             16841..17833
                     /locus_tag="U8116_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN8434100.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17893..19314
                     /locus_tag="U8116_001092"
     CDS             17893..19314
                     /locus_tag="U8116_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEN8434101.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19307..20806
                     /locus_tag="U8116_001093"
     CDS             19307..20806
                     /locus_tag="U8116_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEN8434102.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20845..22239
                     /gene="uxaC"
                     /locus_tag="U8116_001094"
     CDS             20845..22239
                     /gene="uxaC"
                     /locus_tag="U8116_001094"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN8434103.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22398..23081
                     /locus_tag="U8116_001095"
     CDS             22398..23081
                     /locus_tag="U8116_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEN8434104.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23083..23919
                     /locus_tag="U8116_001096"
     CDS             23083..23919
                     /locus_tag="U8116_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN8434105.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23930..25684
                     /locus_tag="U8116_001097"
     CDS             23930..25684
                     /locus_tag="U8116_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8434106.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25960..26301)
                     /locus_tag="U8116_001098"
     CDS             complement(25960..26301)
                     /locus_tag="U8116_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN8434107.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26369..28540)
                     /locus_tag="U8116_001099"
     CDS             complement(26369..28540)
                     /locus_tag="U8116_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN8434108.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28709..29608
                     /locus_tag="U8116_001100"
     CDS             28709..29608
                     /locus_tag="U8116_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN8434109.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29679..30479)
                     /locus_tag="U8116_001101"
     CDS             complement(29679..30479)
                     /locus_tag="U8116_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN8434110.1"
                     /translation="MTIKKKLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGID
                     KTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNS
                     CIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAA
                     GPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLL
                     QKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
     gene            complement(30476..31435)
                     /locus_tag="U8116_001102"
     CDS             complement(30476..31435)
                     /locus_tag="U8116_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN8434111.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         9403 a         6461 c         5565 g        10052 t
ORIGIN      
        1 gtttattgtc ttgaattgaa tcaagacgcc ctacacattt ggtttgtctt actttgttca
       61 gttttcaaag gtctacttca tcgagagtgt ttcccgattc atttttggct gccgtagcaa
      121 cttttatatc atatcagaga gacaaacgat tgtcaactct tttttaaaag tttttttcga
      181 tttcgttttt cgaaatcttt cagcaactca tttatcataa cttatcgttt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc gcaagtggtt ctttttaacg
      301 acttagttat cttatcacgt ctaaaaacat gtgtcaactc ttttttggaa ttttcataaa
      361 atgtttcaat gaagcgcgac aacgttgttt aatatatcat taaaaaaaat aaaggtcaag
      421 ctaaaatatt atttaagctt gaccttatta taatttcaac gattgtcaaa agtattgttt
      481 gcagctgtgt tgccagtttc tatagataaa tcacagcttt tgaaacataa gttttgtact
      541 gcttcgattc gcccgaattc ttttgcagtt atttggcaat gagaaaagca gacatcttct
      601 ataggctttt caggaaatgc tactaaatca aaagctcggg aaggaagtga gtaacctgga
      661 gaaaggcatg cctgtacgtt tttgatgtgt atgtctttga ctttcgtcat ttgctttgat
      721 tcaggtattt cttcagccac tgcttgccaa ctttctggca tttgagcaag gtcattgcta
      781 ttttttctat tgtattcatt atgccagtcc attatccaag aaaaaggaaa ttgtacatca
      841 atcatattca gattttctac cctgatattt tcgatcactc cccctctttc ttttgaagat
      901 ttcattcgaa taccacagtc tgactgaaaa aaatcgatat cgtgaatata tacgtcacta
      961 actccagcac taacttcgct accaatagtt acaccgtatc ctgaacggat tttacatcta
     1021 gcaacctcga ttcttgatga ttttcgattg actcttgcac cattgccatc tctgccagac
     1081 ttaatagcaa tacaatcatc accacaggcc aaatcacatt catagacacg cacatttgtt
     1141 gaagaatcaa tatcaatccc atctgtactt ggtccatcat tgttgcggat gattactttt
     1201 tctactaata catcgttaga ataagtgatt tgcaagttcc aaaaacctga tttctgaaaa
     1261 atcaaatcac gaacaacaac atgttctgca tgatacagta aacaagcgcg tggacgttta
     1321 atagcataat ccgcaatcca acgaagtccc ttttgatcgt attcagctct tgtcccgctt
     1381 ttttgatctt ttccccaata cagctcccac caatggtctc cttgaccgtc gattattcct
     1441 tttccttcta taagaatatc tttcgccgag agaacattca aaatcgctgc tggccatttc
     1501 atttctacgc cggctactcg tgtatcgatt tctggaaatg cttcgatatc tgttctcccc
     1561 ttgaggactg caccttcttc aaagataaga tgactcttat cttttaaaaa aagggctccg
     1621 actaaataaa tgccttttgg aataaataca ggtaagtgtt ctttcgcacc ttgatcgatt
     1681 gctttttgca gtattattgt atcatcttgt tgagtgtcgt ttgttttaaa gttcaaagca
     1741 ttgataatcc ctttttttgt ccgcaatacg tttctcccta tctttcattg atttttttca
     1801 attcttcacc aataaataca gtttttgaac gattcagtgg gtaacctact agcaaaatga
     1861 ttgcggatac aaataagcag aatgctggta acaagtttgc catcgtataa atcctttctt
     1921 gtactaaaac tgattggatg gctccacctg ttgtagacga ttggtaacca atgagtgcca
     1981 gcataaatcc gcctaccgct cctgcacatg cttgtcctac ttttctcgca aaagaattta
     2041 ctccatatac tgttccatct tctcttaagc ctgtcttata ttgatgataa tcaatcacgt
     2101 cagtaatgaa tgcccagacc atcaaattga ataaaccaga tcctaaagta gcgataaata
     2161 aaagaatcag atatacccat gcatttgtta tatgaaggaa atacatcagc aagtacatac
     2221 atgaagaagc aagtaacgca aatacgcttg cttccttttt accaaataat tttgtcagct
     2281 ttgtagcaaa tggtgctaaa actactactg ttccgtaagt aaataaaaga gcaacagaca
     2341 ttgcttgttt attatggaag taatcattga ataaataagt catattcgtc ccagctaaaa
     2401 tctgattgat cacaatgaaa atgtcaacaa tgactaaaat aatcagtgct cgattagcta
     2461 acaaaccttt taccaactga gaagcagata ctttttcgtt tttgacaact cttactcttt
     2521 cagtagtcaa tttacaagtt aacatatatg cgacaaatgc tattgcagca catccgattg
     2581 aaatccaaaa gaaatgattc cctgaaacga cttgttgtcc actcttgtta tgggtataca
     2641 ttaaaatcgg aatgaagaat cctgtagttg atccgccaat cgcagaacca atgcttcgga
     2701 acgtggacaa cgaggtgcgg tcattaggat ttgagctaat tgctgctgcc attgctccat
     2761 acgggatatt gattgttgag agaaaaatcc cataaacaat gtatgaaaca aaaatataag
     2821 caatcttcac cgtcattgga agatgttgga cgaatggcaa aaacaaaatg acagtcacta
     2881 agcaaaatgg atacttcatt tttctgatcc aaggaataaa tcggccagtt tctgttaact
     2941 ctgagacatc acataatctt ccaaccatca catcagcaaa cgcatccaaa attctcgaaa
     3001 tcaagaataa cgtacctacg attgcccctg aaacgccaag aacattcgta taataaatca
     3061 tcaagaaact attgactaaa cctaaaataa aacagttacc tagatcaccg aacatgtagc
     3121 ctatcttatc ggaaatccca aattcctttt tttcttgttt ttatattcac aatgttggct
     3181 ttttattgca ttatcttgac agataaaaaa caaaaaataa tctaaaaaca ttgatgcata
     3241 agattgataa ctttttttac ataacaatat atacaaacaa aaaaattcac taactagtga
     3301 aaataagtta gtgaacctca tttattaaag aattaaatta cagatgatct tttccatcat
     3361 acttgaaaaa ctcaaaatca gcataacttc ctcttttttt atttagatca tgcactgcta
     3421 tcccgataaa atttccagta aaccctcctg agagtataca catgtttttt tgcggaccta
     3481 ctttcttcca ttcttcagtt gatacgtctt tcaaatgaaa ctgtgctgtc tctttcttta
     3541 cagtgacttt caaaaaagtc ttcttttctg tgagtggaat cgtccaatct tctatttgtg
     3601 ctactccgtt ttctgattct atcatacgta ggcattttcc cttgtcctca ttccaagtaa
     3661 cataacaata aatataattg gattcattta agtacagtac cagtccagcc atctgattaa
     3721 aattattagg catgtattca aatgctgtct ccgcatcaaa agtaaaatcc gtttgccgaa
     3781 tagctaacaa atgatgttcg aaggtagatt gaagagattc accggaaatc agacgtaaaa
     3841 aacctggtct acattttgta tcacaccaat cagcatttgg caaaatgcgt ctggcattcc
     3901 agttcttctt tagctctcct tcaaatgtat ccataaactg atgatctttt tcttgttgta
     3961 cctcttcatt tgtctcaatc attatttctt ccatcggagt tgtctggcta tctttaagtc
     4021 ttaaccaatc gtcggacgtc caatacactt cttgaatagc ggtttctctt cccaaaatag
     4081 ccgcaccgtt taatggtctg gtacaaagat atgtcatgta ccatcttcct gacggggttt
     4141 gtactaagct cccatgtcct gtacattgca atgtagaagc aggtttgtca ctagctgtca
     4201 gcataggaaa accaggatca agctcgtaag gaccagtaat attctttgct cggcaaactg
     4261 taactgaatg tcctcttcct gtaccgcctt ctgccgtcat caaataataa tacccgttac
     4321 gaaaatacaa atgtggtgcc tccgttttcg ctaattctgt acctgcaaat attttataga
     4381 tgggaccatc aagcttcttc ttctctgcat catattcttg taatacgata ccgctggatt
     4441 tattcggtgt gtccagacga taatcccata gttcattgac aagccatttt tttcccgttt
     4501 caagatcatg gaacaaagaa ggatcaaagc cgttactatt taaataaatt ggttcggacc
     4561 aaggacctgt gatctcagat gcagtgatca aataattgtg gacatcttta aacggacggc
     4621 ctgcactttt gacatctgtg taaacaagat aaaacaatcc atcagcataa ctgatctgtg
     4681 gcgcccatat acttccattt gtaggatttc cttgcaaaga tacttgagtg gtcaaaatat
     4741 ccgttgtatg tgaccagttg actaaatttt tcgactcata tactcggatt cctggaagcc
     4801 attcaaaaga ggagacaata atgtaatacc gctctttcac tcttaatata ttaggatctg
     4861 gattgaatcc tcttaaaata ggatttttca ctttgatagt ctgcatgatt caggctcctt
     4921 ttctcattct tactcgatat aaactgaatg ccccagctgc gaaataaaaa aatgcaaaag
     4981 gaaacgcaat aaactgagtg ccaatcaaca atatgaatcc tgttataaaa tacaaaccag
     5041 tggcagttgt taagtatttt cctttagcag tcaaaaacaa tgctactgct aaaaaagcaa
     5101 gtgaaataaa caacacaacg gcaaacagac cagtcatgtc ctgtaataat ccaaatgtat
     5161 tttctaaaga caaagttgaa tgatctacag caagcgactg ataacttttt ttaaactctg
     5221 acgcagacat attacttaaa gtaactgcta acccgccaaa gaaaatcaaa gatagactgc
     5281 caccgaccat tcctagcaat agttcccatt ttcttttcat ttctgaaccc cctgcttttc
     5341 taaatgtatc cattcaggtc ttttgatcga tgtttcataa ttgttattac gttcagagaa
     5401 tttgacagtc tgttcatttt tcttgtcatc ccaattatcc caaccagttg ggtgaacatg
     5461 cccacctatc ttacacgctt caaataagac atttgcatac ggacgccacg gtcttcctaa
     5521 atagacgttt ctagtatcag gttctgcagt caaggtacag ctttcaaaac gataccctgt
     5581 tttttgattt ttcggcgtag atgctgctgt gatgtatcca ggatttttac tggctaagct
     5641 atggatatga cactgatgaa aaacggcatc tgccccacca aatatgaaat ccaccgttcc
     5701 ttcaatccag caatattcat aggtctgacg acaatactca ggattggcag aagccggtgt
     5761 acaaaaagga gtaccatctt tttgcaaatc aggcaacgga cccgtgcata aggtatcctg
     5821 ataaccagat agtcgacagt tttttaaaga tacgcgatct cctgaattga ataaagcaac
     5881 agcctgtcct accttttctc ctggacctgc tgtattttcg atgtgaagat tttctaatac
     5941 gacattcgat ccttctaaga aaaaagtagc acttcggaaa gtccctaact ggctaccgtc
     6001 aagatactcc tgttttgcaa aacgattacc ggagataact actgttcctg ctcccacaag
     6061 tttaaaattg gataatctgc tttctatata ttcatcatat cttccttctt ttactatcaa
     6121 gacctttgga tggctatccg gcaggcctgc caaataatcg attccttctt ggatagtttc
     6181 aaaatccgtc ggggtatcta tagcattctt tccaattatc agtttgtctt tcatcctcat
     6241 aaatgctccc ttatgaattg tttccaaata tttctcattt cttgtgtttc catatgaccg
     6301 cctgttaaag aaaaagaaga aaagttttct ggactaccca ttgattgata ctttttgtcc
     6361 agcccttttc tcagcttcag tacgccttcg tccaagcaca tcgtatcgtt ttttccaact
     6421 aagctaagac gagggcgcgg agcgattttt tcttggattg ctaacgttgt ataagctgtc
     6481 aatagatttg gcacatagta ataaaaccca tgatgatcta ggcggcgatg tagcaaaagt
     6541 atttcgatgt ccacctgtgc cgctagatca atacaaactt tgacacgaga atccaatgct
     6601 gtcacccacc agcttaataa gcctcccata gaaagaccaa tcgttgcgag ccgttgagta
     6661 tctgtgtctt cccttgtctc caaataatcg atcaagctga tcacatcata aatacgcatt
     6721 ccccataacg tttttcctgt taataaaaat tgtttgaaca actcgctttc agaaatacct
     6781 ccgcgttctt caaatcccca agcatcaatc gaccaaacag catatcccat tcctgtcagt
     6841 acttctgcaa aagatggaga agctaagtaa ggagcaccat ggatcaattc acttttcccg
     6901 actgaaaaat ctccgccgtg agaatgcagg taaaggaccg gcggaaacgg tccttctttt
     6961 tctggataag catagagggc tggtactgtt tccaattcat ttaagtacaa ttgatagtat
     7021 tcaagcataa aaccgttttg tttttctcgg tataatcgtt ttccactagg ggtatgtctg
     7081 ctagatggag cacctaaaaa atcattcaac tgcataagca aagtttttac gaacaaccag
     7141 ttcatccata ttttctgatt gatttgattg gaatttctca atactgattt ctttagtatt
     7201 ttctaataga aatgctggtc cctcgtgatt ttcaatcgac atctggttaa aggaaacctt
     7261 acccgcaaag ccaatataaa atccttgtct gctcatttcc tctattcctg tcatcatagc
     7321 aggaacaccg ggaactgcat cttgggccat cgagacatcg atattgtcaa atgtgatttc
     7381 ttgtatgaat tgttccgcta atccataaat aaaacctgca gaagcatgga cattccttgc
     7441 cgtgatattc gaaaaatgga tccttctgaa tgctggtgtc cgttcatcta tcggataagc
     7501 ctttttttcc catacatacg gttctttccc tcgcggacca cagaaataat aaagattcag
     7561 tataaatgga cacatcacat tatccatgac tatattggat actcgaatat cttcaacgat
     7621 cccgcctcga cctcgtctag atttcaatcg tattcctcga tcagtttctt ggaagataca
     7681 atttgaaatg gtaatattac gaatactacc actcatttca cttcctaata cgacaccgcc
     7741 atgtccatgg accatcgtgc aatttgtgat cgtgatattt tcgcaagcga tcctttcgta
     7801 cgtatcttca gtccctgcct tgattgcaat acagtcatct cccacgtcaa tatggcaatt
     7861 actaatacgc acatttttgc atgattctgg atcaattccg tctgtatttg gtgaatccgc
     7921 tgggttcaag atcgtcaagt tatcaaacgt tgcattactg caaagaatcg ggttgatcgt
     7981 ccaacttggt gactggatca atttaatatc tttcacagta attcgatgac agttatggaa
     8041 actcatcaat ttaggacgag gataagcgag attatctggt tcatttctaa aagtatgcca
     8101 ccattttttt ccatttccat ctaatgtacc gaaaccagtg acagagatgt tctctacatt
     8161 ttgagcatag atacaggaag cataaacttt tcgatggact ccttcccaac gtgaatgaac
     8221 aacaggataa tcttttggat cgtcagagaa tttcagaacc gctcctgcgc ttaaatgaag
     8281 ttcgacattg ctttttaaaa ataaagctcc tgttagaaat tcacctgctg gaacaactac
     8341 ggtcccgcct ccgtcacttg ctgcagcatc aatggcttgt tgtattgctt cagtattcaa
     8401 ttcatcaatt gatgcgccaa atgtcaaaat atcatacata tttataatgc ctccacttca
     8461 atcaatgctt gcaagaatgc accaacgcct ttttgatcat tacaaataat tggttcactg
     8521 atgtagtaag catatgaacc atctcgctga tctgctccgc ctaaccctgc tacttgacag
     8581 tttttattca agttcagcca gccttctttt gtttccagaa caaattcatc aagtaatcct
     8641 tgatgtgatt tcttcacttg tggaagccat tcttctttgt tcagcagtcc caaacgtatt
     8701 cctttagcca ttgcgtaaac aaacatacta ctgccggaag cttccagata atttcctttc
     8761 cgttttgttt ctgttgttac ttgataccat gtcccgctgt tttcttcttg atatggaagt
     8821 aatgcagcca gtgtcttttc tagaatttct ttcagcaaag aacaatctgt ttcttcatga
     8881 agtaattcat aggtatctgc tgcagccatc aaataccagc caatcgatcg gctccaaaaa
     8941 tgatgggata gacctgtttt tggatcagcc catggttgga ccttcttttc atcccatgca
     9001 tgaatcagca aatttgtttt tggatcgaat agatgtttat aactgatttc aaactgtctc
     9061 aaaacatctt ggtaatcttt ttcttcacta aaattctgga taaattcagc ataaaaagga
     9121 gaacccatat ataagccatc caaccaaatt tgataaggat aaatttcttt atgccaaaaa
     9181 gctccttcgg atgtgcgtgg atggtttgcc aactgctggg aaaggagttc tgctgctttt
     9241 ttatattttt cttcgcctgt ttctttgtac aataaaaaga aaagctttcc tgtatttaca
     9301 tggtcgatat tgtattcttc aacgctgtag ccacggatcg ttccatcttc ttgcacgaaa
     9361 tagtcgatat tgtcttttat gaaatcaaaa taaatttttt cttgggttcg ttcccaaagt
     9421 aattggaacc ctttaagtac gacgccatag tcataagacc attttccttt ataccacttt
     9481 tcttcatata agcgaggtgt tctttccatg atactttttg ctaatttctt tgaccagttt
     9541 tccattattt gctctccttt aaaaattaaa ataggatttg ggacaaatac caagaagtat
     9601 ttgtcccaat cgtctatatt actgtgccgc tgctttgtat gcttcctcgt actcttgtgt
     9661 gattcgatcg cctccttgtg ctttccagtc ttctacagct tttttgaatc catcttcgtc
     9721 aagctgtccc atgatgtatt gcaccgtagc atcaatgacg attttttcaa tttctgatcc
     9781 tacttcattg ttcgtcggtg aatccaatgg cacagaagga tccattacag caaattcttc
     9841 attttctttg atcaattgat tagagaattc tttttcaggg tctgtatctt tgaaagagaa
     9901 cgtcacttca ctaggacgag agcttgataa cggttgcacc tcttgttgcc aaagatccat
     9961 atcgataaat tcgatagaac catcatcatt caattcataa tgcgttcctt cgataccgcc
    10021 agtcatcaat ttatacacat cttcttctaa aagatcatca acaaattgaa gtaatttctt
    10081 caattgtgct tcgtctttta cttgagattt gggaaatgct agcaagccgc caataccatt
    10141 tccttctgac caaacatgat aaccgccgcc ttcaccatct gagatcttgt ttacaggcac
    10201 taattccata tcatcttgaa taccttgacc catcgtcttc agattcttga tatccaccat
    10261 tccagtgtag attccagttc ttccttgtgc aaattgttct tgctgatcag tcttttgggt
    10321 aactgcaaag tcttgtgcca aatagccatt ctcaaataga tctcttgaaa aattcaaagc
    10381 tttcatatac tcttctgtat caaattctgg agtgaatttg ccattatcgt ctacagccca
    10441 gccatttggt gctccaaaat aagaagtgaa aagtttaaag ctggtataac gaatatcact
    10501 aggcgcacga tcgccaaaac caactgtgtc gtccacacca tttccatcag gatcgttttc
    10561 tgtaaattgt ttagctactt cataaagctc atccatggta gttggaacat ctagtcctag
    10621 attatccaac cagtcttttc gaatgactaa gccagcacga gcgtagtctt tttgtatcgg
    10681 tacgccgtac aaggcaccgt cgatactcgc agcttcgatt ctatcttcag aaatttgact
    10741 taaattttta tagtctttta aatattctga tacatcccag aatacgcctg attttaacgc
    10801 attgcggaca gaagagtttt tcaacatcgc aatttgaagt gtaacgatat ctcctagttc
    10861 atcagaagcc aatgcagttg ttaatctttc ttcttttgaa gcatcaggaa tccagttgaa
    10921 tgtcagttcc gtatcggttt tttcttcgat caagttcaca atcgtgtctg taggcggtga
    10981 agcatgatgc aaaatattta accaagtgat tttagctttt ccatcttctg tctctgaaga
    11041 actgttactt gaactgctgc cgcatgcggc aagtgctaga attcctgcaa aactaagacc
    11101 taattttacg agcgttgctt ttttcataaa ttttctcctt cactgtttta ataatatttt
    11161 tgttttcttc tgttttttct gtatatgagt tcttccattc caaatggcaa aatatagaat
    11221 ttgctgtcca cgtgactgcg gaaaagaaga gactgccacc gataaaggtt aaaacacctg
    11281 gcattgtttg gatgatcatc agataaatcc ctaccatact taatatcaac actaatgagt
    11341 aatgaagatg tgagaatccg aataaaaatg ccattttcat tttgaaaaaa tatcctttcc
    11401 aatcataatg agccatgatt gggaaaatat agagtgctga gagcacatat atgaacgcag
    11461 caatatacaa agcgatccta agataccaat tgtgtatgaa agaaagatcc acaagtaaaa
    11521 caatcccgat ggcgccatat atccagctca tcagcaaggt ttcacgatag ttttctttaa
    11581 aatatgtgaa gtacgttttc gtcaaaggaa acgtttcttt tgagcgcagc cattttctaa
    11641 tcactgcaac catcgcatac gatgcaggac cgaaagtaaa aactcccaaa cctgcgatga
    11701 ctaaaccgcc ccacagaaaa ttcaaataaa ccaactgtaa aatacgattt agccttccgt
    11761 ttattttatc taatgactgc agcatatctt tccctctctt tctatcttag taaacgccgt
    11821 cttctcctag ctttttggca atcttattag ctaaaattac cataaacaat cctacgaccc
    11881 ctttaaacaa tcccattgct gtactgaaac ttaactgtcc attcttcaat ccagctgtat
    11941 aaatataggt gtcgaaaatt tctgcaacgc ttcgattcaa tgaattcaat aacaagtaca
    12001 tatgctcgaa ccctaagtct aatgtctgac cgatttttag aatcagcaag gtaatgatta
    12061 ctggacgaat tgccggcaat gtaacatgcc atgttttgcg taagcgtcct gctccatcca
    12121 tttctgcagc ttcatacaac tgtgtatcga ctgcagttat cgctgaaagg tagatgatcg
    12181 tagaccagcc caactctttc cagatcactt gtccgatata tactgtgcgt gtccattcag
    12241 gggatgttag aaaactgatt ttttcaaatc ccattgttgc aaggaattca ttcaataccc
    12301 ctccgtcaac attcaaaaat acataagtaa tcgaaacaat gattacccaa gacatgaagt
    12361 gagggatgta aatgatcgtt tgaatcccgt ttttcaatcg tttgttcgtt acttcattta
    12421 atagtaaaga caaaatgatt ggcatcggaa aaaacacgaa gatatttaat ccgaacaaga
    12481 tcaacgtatt tcttaataat gtaaagaatg tcggttctgt aaacaaacga atgaaatgtt
    12541 tgaaaccgac ccaaggactt cccattattc ctaaaaatgg ttgataatct tgaaaagcaa
    12601 taacgagtcc ccccatagga agatacctaa aaatcaagaa ataaaggact cccggcagaa
    12661 tcatcacata taaaaattta ttcgttttga ttcctgcaat tatttttttt cgtcttctga
    12721 gcttttcttc ttttttcgat agcgtcagtt gaaatggttc ttgtttcttt acactttcca
    12781 taagcccctc cttagttttg tgaatgtttg ctgcttatga cttcatgata gataactatc
    12841 tgaattacgt atataatcat ttttaccgta agtaaaaacg cttacatagc aatgattttt
    12901 gtgctataca aaacgataaa gtgtattttt tcacaaataa actacgcata gcaaaccttc
    12961 tatttttttc tatctgacac ttagcatagg aaatgattat atacgtaatc tttcaccgat
    13021 gataaattga acatatagaa acagaaacat ttaatttatt ttgaggagga gcctatctat
    13081 atgaaaaaga aagtctctat aggagaaaga agttttacac ttttcaacaa tacgtttcta
    13141 attttgctag ctttgatctg tatcgttcct tttttaaata tcatcgctac atcttttgct
    13201 tctacacaag aagtcgtcgc gaaaaagttt atcctttttc ctactacctt ttctttagac
    13261 gcgtatcgct acattctttc tacgccgacc atttttagag cacttgctgt ttcgattggt
    13321 gttacaggtg tcggaacgat cgtcagtatg tgtgcaactt cactgatggc ctacggtctt
    13381 tccagaaagt atttgtttgg tcgaggtttc gtcaactttc ttgttgtatt ttctatgttg
    13441 ttcagcgggg ggatgattcc aacgttctta gttgttcgtt ctctagggct ggtcaattct
    13501 tactgggcca tgatcctgcc tgtggctgtt aatgcaatga atatgatcat tatgagaaac
    13561 tttttccaag ccttgccgga tagtttggaa gaatcagcaa aaatggatgg ttgcacagat
    13621 tttggtgtat ttttcaaaat tatgctgcca ttagcgttac cgtctattgc tactatctcg
    13681 cttttttatg ctgttactta ctggaacact tatatgactg ctattttgta tatcaacgat
    13741 tcatctaaat ggccgataca gattctttta cgccaaatcg tcattgtatc aagtgggatg
    13801 caagcagaaa gtagtgccgt tgatgtgatt ccaccagcac aaacagttaa aatggctgtt
    13861 atcgttattg caacagtgcc tatgctgatt gtttatcctt ttgttcagaa atattttgtc
    13921 aaaggagcac tcgtaggttc tgtcaaaggg tgattatgta ctgaacgcaa aatggtagtt
    13981 gtgtcagaag tggtcagctt caagaaataa gaaggcattt acgaaaattg cttcttaaat
    14041 ttttgtgaaa tttcggctta tttttcgaag aatctactcc tggaacaccg tgtatcagag
    14101 gttgtgaaag agacgttcag ccttgagcga tgataaaaac tctcacaaac attgcgacga
    14161 gtaacccaca ggagcaataa gaagcgattt tgaaaaatag cttcccatat ttcacaaaat
    14221 tatgccttat tgtcgatagc ctgtctcttg aacgccattt attcgtaaaa aagaactgtg
    14281 acaagacttt tgtcacagtt ctttttgcgt gcttagcctt gaaatttaac tatccataaa
    14341 tacaaatact agggttattc taacttcttt tattgcttga atcagtccac aagaaaaaca
    14401 gcctcttgat tcacaaatat ttttttcgat attctcccgg cgtaatcgat tccttctttt
    14461 taaagaaacg aataaaattt tgcggatttc gatattgcaa tctttctgca atctctttta
    14521 cggagaggtt agtctctttc aaccattttt ttgctacctc taaacgatga ttttgaacaa
    14581 aatcaccaaa tttttctcct gtttcctttt taaatacatt acttaaatag ttagggttat
    14641 aatgcaacct atcagcgatc acatctaaag acagatcacg atcatattct gtgtagacga
    14701 tatgcataat attctccgac aaatttttaa attcatgatc agtgattgct tgagagcatt
    14761 ctgtgattgg cagaactaat tgttctaaaa caagtttttc tagttctttg ggatgataat
    14821 tgttcagtgc ttttacataa atttgtttca tgttatcaaa gttttttgaa tctactccca
    14881 ataactgtcc taattgaacg agttcattga tcaagcggac cgaaactact tcccgactca
    14941 aaggattttt attttgtgca aacaatgcat caatcaattc atgtactaaa ttcttcactt
    15001 cctgatttcc agaacgaatg gcttcaaaca atcgattttg ttcttctatc ggatatctaa
    15061 taagtgtttt ttcatgagca gcaggaacga tttcctgata aaaaataatt gaacttggac
    15121 cagtatttac acgataatac agggcttctt ttgcccgatc aaccgattgc ttgctttcat
    15181 tcagcgtttc gaaccggtta ctgatgccta tactgatagt taaattcaag tattctttta
    15241 cacgtttttg aatgagtcgg caatactcca tgactttttt gcttgcttct gcatcatctt
    15301 tatccattcg ataaatagtg gcttgcattt cttcattcaa aacgatcggc agcattcgat
    15361 tttcctttgg cacaatttcc gtgatcatgt tgttgatcgc taaaagtagc aaatcacgct
    15421 ccccaccgtg actatcttct aacagatcga tttgaaccaa agctgtataa tagcagtcat
    15481 tttgagaact ataaccaaat tgctgcaacc gctgatctag ttctcgcctt tctactcgat
    15541 ttctaaataa actaagtaca aatagcgttt ccagctgtgg cttttgggta actaagtgtg
    15601 cacttaacgc ttctttttct cctatgatcc gactgactga ttgagcgacc aaatctagtt
    15661 ctttcccact gaatcctttt gatttaagat tcagacgttc ctgtatttga gaaatcggac
    15721 gggcaaatct gtccgaaaat aagtaggaaa gaagaccaat gagtcctacc agtgttaaag
    15781 acactgcaac tagcccgatt ctcagattgc ggatcattgc gccaattgca ctagagtcaa
    15841 cttctaatct gtaaatccaa ttattataat ctgatttcag tactatatac gttttatctt
    15901 tttctcttat cactttcagc tgattgtaat ctagatccac gtccgttaat acagttggtt
    15961 gctctttatt ttttactgtc tgagtagtaa aaaggttgcc aacttcgttt tcaatagaaa
    16021 gtatgtcttc tccactttca ccaacaactt gttctattgt atgtttctct atcgtagcaa
    16081 ttcccaatgc atatttttct ttttcaaaca ttggcaaaga aatcaccatt tcgattcctt
    16141 tgtttttctt ttgccaaaat aaattatttt tgttatttat gtacttctgt tgatactttt
    16201 caatttcctc ttcggtcagt tgactcaacg atccattgat tactccccat ttaccagcca
    16261 aactgactaa atcataatta ttatcatcca taacaatcgt ttctataaaa tttagttctt
    16321 tcctgatttc agaataagtg gagtaatctg tatatgtcag cggatgcttg aatatgtccg
    16381 agaaattttt ggtcgaacca tacgtagaaa acgaatattc aatcgttcta attttccatt
    16441 caacgttagt tttaacttgc ctgagcaaat tctttttgtc tgttaaataa ctcgattcga
    16501 tcgattcata ggtattctgg taaatgaata aactaaatcc tatgacaagt acggctccta
    16561 ataaaaacat cggtaaaaat attttataaa atactgtact tttcttcatt ttcttcctct
    16621 cctcctttta aaataaaagg ctttcatttt tctgtaaaat tgatactcat ctaattaaat
    16681 gagcattccc cgctttcaag aatagcataa ttaaaaataa aaatccaagt aatttttaac
    16741 gtgtgcaatc ttttcatatc aaagatctat tgacttatcg tagttatcgt aatatgatga
    16801 atgcgttaac aaaaagtgaa aaagtaagga aaatgatgga atggtaacga tcaaagatgt
    16861 cgctaaagca gctggcgttt ctcatacgac agtttctcgt gctttgaata acagcagttt
    16921 aataaaaaat gaaacaaaac aaaaaatacg acaactagct aaggaaatga attattcgcc
    16981 aaactatagt gcaaaaggtt tagtgaatca aaaaaaatat ctaatcggac tatttttctc
    17041 aagtttgcat aaaggaactt catcaaattt cctagcagat tccattgcag gcatccattc
    17101 agtattagac acaaattaca gtttatctgt tgaaagtatc gatgttgttg atttaacaga
    17161 aatcaacctg caacgctacg atggactgat cgttatgagt caaagtgatg atgatcagcc
    17221 tttcatcgac tatctcaaag aaaaagaatt tcctttcgtt gttgtcaatc gtcacatcaa
    17281 tgatccagcg gttatcaacg tcactgccga tgatgcggcc ggtgttatgc aggcagtaga
    17341 ttacggtatt ggatgcggac atacgaagat cgcttatatt ggcggaaaga aaaattttcg
    17401 ttcgacaaaa gaacgggaaa aaggggtatt gaaaagtcta aaaaaagcga gcatccctgt
    17461 caatccagcc tattttttaa caggagatta cagcttagaa agcggcctcc aaaacatgga
    17521 atacctatta tcactttctg acctgcctac tctggttttt tgcggcaacg acgacatagc
    17581 gattggtgca ttgagagctg tacatttagc tggcctccgt gttccccaga acatttctct
    17641 gatcgggttc gatgattctc cagtcgtctc ctatctcaat ccgccgctta ccaccgtacg
    17701 taagcctttg aaagaaatgt gtcaagaagg aacaaagctt ttactagatt tgatcgaagg
    17761 caggacgatc gaacaatcta aaattgagct ctccaccttg ttgatccttc gtgagtcgat
    17821 caattacttg taaactacaa gtaattttat ataaaaatgt taacgtgtgc ataacacaga
    17881 aaggaagttt atatgaaaaa actgacaaaa gaattagtga aaaaaaacga agcacctgta
    17941 aaagttcttc agtttggtga aggcaatttt atgcgtggat ttattgattg gcagattcag
    18001 cagctgaata accaacaatt gtttaaaggt aatgttgcaa tcattcaacc tttagaacaa
    18061 ggtcttggaa atatgatgaa ccagcaagat aatttatata cggtcatttt acaaggactc
    18121 agcaatggcg aaatcgttga tacttccgag attatcaccg tagtagaaca aatgatcaat
    18181 ccttatgatg aatgggatgc ttatctatct ctagcagaaa atgacgactt ggcctatatc
    18241 gtctccaata caacagaggc aggaatccaa ttcgtggaga ctgataattt aacagatact
    18301 cccccacaga gcctcccggg caaactgaca gcctttttat accgacgttt caaattaaac
    18361 aaagctggct ttaccatcat tccttgcgaa ctgattgaca gaaacggcga acgtctaaaa
    18421 gaaatcattt tacgctacgc agaaaaatgg gatttagaac ctgcttttgt tgagtggtta
    18481 gaaaaagaaa atgttttctg ttgtagttta gttgatcgca tcgtcccagg ttatcctcgc
    18541 gaagatgcag gtgttttcaa tcaacggcat aactatgaag atcaattgat ggtcaaagca
    18601 gaaccattca tgctttgggt gatcgaaggg cctaaagaat tggagaatca actaccattc
    18661 aaacaggctg gcttgaatgt catcatcaca gatgatatga ctccttatcg tgaacgtaag
    18721 gtgcatttgc ttaatggacc gcatacagcg atggtccctc ttgctttgtt ggcggaattg
    18781 gaaactgtag aagaagtaat gaaagatcct ttattctccg catatgttga tcagttattc
    18841 aatctagaat tgattcctat gctttcttta cctaaagatg aattagccat ttatgctgac
    18901 caaatcaaag aacgtttctt gaatcctttt gcccatcaca aattagaagc gatttcatta
    18961 aatagtgttt caaagttcag cacccgtctg cttccagtgt tcaagaagta tatcgaagaa
    19021 caaaatcagg taccaccgtt aatcactgtt tcactagctg cgctacttct tatgtatcga
    19081 ggcgatcaag taaagccaca tgatgatgaa aagacgataa gcgaatttac tgatgcttgg
    19141 tctgataatg gaacagctat tcctcgatta ttgcaaaacg ctgcgctttg gggagaagat
    19201 ttgtctcaaa taccgaatgt gacagataca gttcaagaaa tagctgcaaa gatcgatcaa
    19261 aaaggtgtcc ggtcagtatt acaagaaatg aaaggaacga tggtccatgc ttgaaaccct
    19321 gcaaccaaca atgaaattaa atcgacgaga cagtgtggtc gtcgcattaa cccctattcc
    19381 aaaacaaaca caactgacga ttgatcagca aacgatcacc actcttgaag atatcccgca
    19441 aggtcataag attgcattgg gtgatttgaa agctggagac aacgtgatca aatatggcta
    19501 tccaatcggt catgtgacga cagacgtaac tgccggtcaa tggctgcaca ctcataatgt
    19561 aaaaaccaat ctttctggtg aattggatta tcgatatgaa aaagatgtcc atccaagtca
    19621 ttaccttttt gagaaccgaa catttcaagg ttacttgcga aaaaatggaa aagtagggat
    19681 tcgtaatgat ttgtttttag tacctaccgt tgggtgtgtg aatggcattg ctgaactgat
    19741 cgtcaaacag ttcaaggaaa aacatccaga tctgggacag tttgaccata ttacgatttt
    19801 aaagcatcct tatggctgtt cgcagcttgg aaaagatcat caaaatactc gagaaatttt
    19861 agcggacgca gtaaatcatc ccaatgccgg aggagtttta gtatttggat taggttgtga
    19921 gaacaataca gttcctgaat tcaaaaaaat tttaggtgaa tatgatgaag aacgagtgaa
    19981 atttctagtg gctcaagatg tttacgatga gatcgaacaa ggtgtggctc tattagaaga
    20041 gcttttaaca gcggcggaaa atgaccagcg catatcagtc ccgctctcta agttgaatgt
    20101 cggtttgaaa tgcggcggtt ctgatggtct atcgggaata actgctaatc ctttacttgg
    20161 tgcattttct gactacttga ttgctcaagg aggcagcacg atcttaacag aagtgccaga
    20221 aatgtttggc gcagaacaag tattgatggc acgagcggaa aatgaagaag tttttgactc
    20281 aatcgttgac ttgatcaatg acttcaaaca gtatttcttg tcttacgggg aaccagttta
    20341 cgaaaaccca tctcctggta ataaagcagg tgggatcact acattagaag ataagtcttt
    20401 aggctgcacg caaaaatcag gaagctctcc tgtcgttgat gtgctacaat atggcgaaaa
    20461 aatcagaaaa ccagggctta gtttattgca agctcccggg aatgatcttg tagctgcctc
    20521 tgctcttgct tcatccgatt gccagttggt cttgtttacc acaggaagag ggactccgtt
    20581 tggctcatat gtcccaacgg tcaaggtatc tacgaataca actctttttg aacgcaaagg
    20641 tcattggatg gattttaatg caggcgtctt attagaacaa ccaatggaag taatcttaga
    20701 atcgtttgtt caaaaaatca tcgctgtagc tagtggagaa gaaacgaaaa atgaacaaaa
    20761 tgacgttcga gaaattgcca tttttaaaaa cggagtaaca ctttgatttt cataaaacag
    20821 tataaaaaga aagaaggaag atttatgttt ttatctgatg attttttatt aaaagacgat
    20881 tgggcgaaaa agctttacca ttcctacgca aaaaacatgc ccattattga ttatcactgt
    20941 cacttagatc caaaagaaat ttacgaaaac aacaatttca gcaatctgac agaagcttgg
    21001 ttaggaggcg atcattacaa atggcgcttg atgcgagctt gcggcgtgcc tgaagaaaaa
    21061 atcacaggaa atgcttctga ttttgaaaaa tttttagctt ggtgtcaaac cgtaccaaaa
    21121 attatcggaa atccgcttta cagttggtca catcttgaat tgaagcgttt cttcggtatt
    21181 gacttgttga taaatgaaga aaatgcagag aaaatttgga cacaagcaaa tgaaattttg
    21241 gcagctcctg attttcgccg tcgggaaatc gcaaaaaaat cgaatgtaga agtgatttgt
    21301 acaacagatg atcccgcaga cgacttgcac tatcatcagt tgcttgctca tgaagaacca
    21361 gatttgaagg ttctaccttc attccgtcca gataaagcac tcaatattga aaaagaagga
    21421 tttaatgcat gggtcacact actggaagaa gcagctgacc aaacgatcac cacctatcaa
    21481 gaactgatag aagtcttagg gcagcggata gaatacttcc atcaacatgg atgtcgtgtt
    21541 tccgaccacg ctttagacat cttacgctat aaagaagcgg atgaaacagt attagaagag
    21601 atttttcaaa aagcacgagc taacaaacta ttatcttcag atgaaatcga tgcttatcgt
    21661 acagaaacac tgatacattt gacccacttc tatcattctc ataattggac gatgcagctg
    21721 cacatccatg cttatcgtaa ctgtaataca caaatgttgg aaaaattagg gcccgatacc
    21781 ggctatgatg gaatgaatga tctttctttg actattcctc tgcaaaaatt attgaaccgt
    21841 gcagaagaaa cggatcagtt gccaaagacg atcctttatt cattgaatcc gaatgactat
    21901 cctgcgattt tagctttgat gggctgtttc caaaaagaac agaatggaaa actgcagtta
    21961 ggttctggat ggtggtacaa cgatactcga gctggtatgc gcgatcaatt gacacaattt
    22021 gctgatggta gtgctttggg gaattttgtc ggcatgctga cagattcaag aagtttcctg
    22081 tcctacacta gacatgaata tttccgtcgt gttctttgtg aatttttagg cgaaatggtc
    22141 atgcgaggag aagcacctga agatgaacaa cttttaggaa gtctagttca agcgattagt
    22201 tatacaaatg ctaaaaatta ctttggcttt tttgcctaat tgattaatag taacagtaac
    22261 gaatcaaaaa caggatagtg aaataacgga atccgtttat ccacgtttta gagaagaaga
    22321 cggaccaaat ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt
    22381 atgaaagaag agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa
    22441 aagatgaaaa caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac
    22501 ccgagatcca gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg
    22561 aaggaaggtt agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat
    22621 ttggacataa cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt
    22681 taaaaaatct gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac
    22741 tgacatctgt gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg
    22801 gagactatcc aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg
    22861 tgttctcaag atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct
    22921 acctgcatct tatacctgat acttgtaaaa attacccaga aggactaaaa gacaataccc
    22981 actttagtcc agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat
    23041 cccgtctgcc tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta
    23101 ggcatccgtg cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca
    23161 cagcttcact tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa
    23221 ttaactgaca tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat
    23281 atcgaacttt ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt
    23341 gcacaagcat tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag
    23401 atgattgcta cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa
    23461 gaagcttatc agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt
    23521 ggcgtaactg ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct
    23581 aaacgtttaa tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat
    23641 ttgatgcata aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat
    23701 ttacatggtt atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc
    23761 cgcatggtac ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa
    23821 tatgagcgtc ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa
    23881 gcaatagaac acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg
    23941 aatacagaaa agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta
    24001 tcagggaaat tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc
    24061 atcttggtta atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc
    24121 ttctcagttg caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa
    24181 tatattgacg aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc
    24241 tggactaaga gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga
    24301 attgcaatct ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt
    24361 gtcctctcaa gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg
    24421 tatcacgctt tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc
    24481 cgcattcgtt actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca
    24541 agaaatggaa gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg
    24601 agctcatggg ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta
    24661 tgaaaaagga ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag
    24721 ttttcagata tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg
    24781 catccaaccc ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt
    24841 ttctggaatg atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat
    24901 tggcgaactg ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca
    24961 tttggatgga aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc
    25021 gattatcgat catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc
    25081 atctggctct ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc
    25141 aggaaaaatt tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa
    25201 ttatttagcc gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat
    25261 cacttatggc ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca
    25321 actttcttca ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg
    25381 caacaagctg tctggtggcc aaaagcaacg tatctccatt gcacgagcgt tgatacgtca
    25441 gccaaaaatc attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa
    25501 aatccagcaa gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg
    25561 gctttcaacg atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga
    25621 aataggtact tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga
    25681 atgacaaaaa ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg
    25741 gtgttctcct gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt
    25801 tttttgaaat tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa
    25861 tggtgttcaa gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga
    25921 acgtctcttt cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg
    25981 aactggtgtc atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt
    26041 ttgcatcaag tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt
    26101 cattgcatct tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg
    26161 catcgcttgg aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta
    26221 cgcttctttc aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc
    26281 gctatcgtag atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt
    26341 aacatggggt tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt
    26401 tagccgcttc accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc
    26461 cttcttttgc ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat
    26521 tagaatttcc tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg
    26581 catctgctac actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt
    26641 tgaagacttt cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt
    26701 cttcgtaacc catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt
    26761 catttgcttt tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat
    26821 aacctgcagg attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg
    26881 tcatttcatc ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga
    26941 cgatcaccgc tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac
    27001 catcattggc aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca
    27061 gtccaccaag ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt
    27121 ctgctttatc atatcctttt tgtaaaccga tctcatgcaa aagccataca gtaggcaaat
    27181 tcaaactttg tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat
    27241 tatgcgcatc gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag
    27301 cttccagtgc tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca
    27361 tctgtgtagc aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt
    27421 gtacaccacc tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg
    27481 aagcatttgc tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat
    27541 cctgatctaa agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac
    27601 catactcgtt aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag
    27661 aatctgaatt atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt
    27721 gttcttgttc ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc
    27781 gatcattcgc attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc
    27841 ctgccaaagt agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc
    27901 tggaagcgtc ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg
    27961 tcaagatttc ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg
    28021 cttttcgatc aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga
    28081 tcgtacttcc tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg
    28141 caatcccttt gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta
    28201 ctgcatcttg gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt
    28261 acagtgtgcc agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg
    28321 attttaacgt ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta
    28381 agacacagat caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc
    28441 gctttcgcca tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca
    28501 aagcagcttt tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat
    28561 ttgattcaat cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta
    28621 gttcaatatg tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg
    28681 taagtttttt cttaaatatg ttttaaaaat ggtacaatta gtctgcgatt gcactgaaag
    28741 gagtacactt gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg
    28801 cgacttgctt gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg
    28861 taaaaacgtt tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga
    28921 ccagatcaca ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga
    28981 tgcgaagcac atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc
    29041 aggaatgaag actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc
    29101 tgcttatctc gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac
    29161 aagcggtgcc gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt
    29221 ggaacaaaaa atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga
    29281 tatgaccatc accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga
    29341 tgataaaaat ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca
    29401 aacacaagtc tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc
    29461 tcataaggga catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact
    29521 gatgctccat gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg
    29581 taaagcttta cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat
    29641 aatgataaga ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct
    29701 gctaacagca aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat
    29761 tcacttactt gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt
    29821 ttttgaagga gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct
    29881 aacgtcaacg tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca
    29941 ggatgatctt gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct
    30001 gctgctaatc cttctaaaca atctttatga taaggacaag taccttcata atggtcatct
    30061 ggatggcgtt tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct
    30121 tcattgatca cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt
    30181 tttccttgtg ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc
    30241 caagcagcag gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac
    30301 ccttttttgg gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc
    30361 gggccgaatg agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc
    30421 tgatccaatg tttcttctgg tgttgtggta gggatactta attttttctt aattgtcaaa
    30481 ttaagttaga cagatcatca atacttacgt aactcaaaaa tacttccaaa ggtgttcgat
    30541 aatccagtga ttttctagga atattatttc gtttagatgc gacagattga ataaaaaatt
    30601 catctactga attgaaatcc atagatttca ataaaccatc tctacgtaac aatccgttag
    30661 aattctcgtt taggcctctt tgagacggcg ttcctggatc agcgaaataa atggcaatat
    30721 cattgacatt actgatctgt ttccaatttg aaaattcctt tccacaatca aaagtgatgg
    30781 atttgaatag gtttttcggt acagattcaa accattgatt taattttttt tcaatatcaa
    30841 tcgcttgtct accacacggt ttcaatgtga tgataacttt tgataatcgt tcaactaagg
    30901 taattacagc acttttatgt ttcagaccta cgatagtgtc accttcaagg tgaccaaact
    30961 catttgagaa ttggctataa tccttttcac gttcatgaat agagcggcgg aaagtttgtt
    31021 ttccacgtct ttcttgatgt ccattcggtt tacgcttgcc tttcatcggt aagtcagaag
    31081 aatcaaatag cccctttttg aacatacgat aaatggtacg agcagaacaa ctaataggaa
    31141 acgctgcacg accaacaatc acatcgggtg tccatccttg aacaaccttt ctttgaatat
    31201 attctgattg ttcctcgggt aaaacaagcg gacgtctacc acagtttgat ttgtttttct
    31261 tatactgttg ataatattct aaggctgatt ttccttgctt gaagaacaga tatactttat
    31321 ggatcgtttg tcttgaacga ttcaagcaat gggcagtttt agcaacagat tgattcattt
    31381 tgaaataaga ctctattatt acaagttcat ccgttgtaag atgggtatag gtcatttgta
    31441 ctcactcctt ataattcttt ggtcggaact attttgagtg t
//
LOCUS       DASNNU010000026        30646 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.026, whole genome shotgun sequence.
ACCESSION   DASNNU010000026 DASNNU010000000
VERSION     DASNNU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.026"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..299)
                     /locus_tag="U8116_001103"
     CDS             complement(<1..299)
                     /locus_tag="U8116_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN8434112.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPL"
     gene            complement(299..529)
                     /locus_tag="U8116_001104"
     CDS             complement(299..529)
                     /locus_tag="U8116_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN8434113.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            816..1154
                     /locus_tag="U8116_001105"
     CDS             816..1154
                     /locus_tag="U8116_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434114.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1359..1598)
                     /locus_tag="U8116_001106"
     CDS             complement(1359..1598)
                     /locus_tag="U8116_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN8434115.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1574..1921)
                     /locus_tag="U8116_001107"
     CDS             complement(1574..1921)
                     /locus_tag="U8116_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN8434116.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2653..3234)
                     /locus_tag="U8116_001108"
     CDS             complement(2653..3234)
                     /locus_tag="U8116_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN8434117.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3417..4043)
                     /locus_tag="U8116_001109"
     CDS             complement(3417..4043)
                     /locus_tag="U8116_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN8434118.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4065..5396)
                     /locus_tag="U8116_001110"
     CDS             complement(4065..5396)
                     /locus_tag="U8116_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEN8434119.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5551..5952)
                     /locus_tag="U8116_001111"
     CDS             complement(5551..5952)
                     /locus_tag="U8116_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN8434120.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6038..6244)
                     /locus_tag="U8116_001112"
     CDS             complement(6038..6244)
                     /locus_tag="U8116_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434121.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6339..7841)
                     /locus_tag="U8116_001113"
     CDS             complement(6339..7841)
                     /locus_tag="U8116_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEN8434122.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7859..8314)
                     /gene="argR"
                     /locus_tag="U8116_001114"
     CDS             complement(7859..8314)
                     /gene="argR"
                     /locus_tag="U8116_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN8434123.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8412..9029)
                     /locus_tag="U8116_001115"
     CDS             complement(8412..9029)
                     /locus_tag="U8116_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEN8434124.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9250..10623
                     /locus_tag="U8116_001116"
     CDS             9250..10623
                     /locus_tag="U8116_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN8434125.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10698..11810
                     /locus_tag="U8116_001117"
     CDS             10698..11810
                     /locus_tag="U8116_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEN8434126.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11882..13519)
                     /locus_tag="U8116_001118"
     CDS             complement(11882..13519)
                     /locus_tag="U8116_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEN8434127.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13687..14295)
                     /locus_tag="U8116_001119"
     CDS             complement(13687..14295)
                     /locus_tag="U8116_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN8434128.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14679..15830)
                     /gene="queG"
                     /locus_tag="U8116_001120"
     CDS             complement(14679..15830)
                     /gene="queG"
                     /locus_tag="U8116_001120"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEN8434129.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15849..16601)
                     /locus_tag="U8116_001121"
     CDS             complement(15849..16601)
                     /locus_tag="U8116_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434130.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16742..17692)
                     /locus_tag="U8116_001122"
     CDS             complement(16742..17692)
                     /locus_tag="U8116_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN8434131.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17693..18532)
                     /locus_tag="U8116_001123"
     CDS             complement(17693..18532)
                     /locus_tag="U8116_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN8434132.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(18529..19275)
                     /locus_tag="U8116_001124"
     CDS             complement(18529..19275)
                     /locus_tag="U8116_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN8434133.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            19513..20883
                     /locus_tag="U8116_001125"
     CDS             19513..20883
                     /locus_tag="U8116_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN8434134.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20938..21834)
                     /locus_tag="U8116_001126"
     CDS             complement(20938..21834)
                     /locus_tag="U8116_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN8434135.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21948..22118)
                     /locus_tag="U8116_001127"
     CDS             complement(21948..22118)
                     /locus_tag="U8116_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434136.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(22115..22792)
                     /locus_tag="U8116_001128"
     CDS             complement(22115..22792)
                     /locus_tag="U8116_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN8434137.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22883..23788)
                     /locus_tag="U8116_001129"
     CDS             complement(22883..23788)
                     /locus_tag="U8116_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN8434138.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23963..25417
                     /locus_tag="U8116_001130"
     CDS             23963..25417
                     /locus_tag="U8116_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN8434139.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            25433..26464
                     /locus_tag="U8116_001131"
     CDS             25433..26464
                     /locus_tag="U8116_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN8434140.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            26471..27658
                     /locus_tag="U8116_001132"
     CDS             26471..27658
                     /locus_tag="U8116_001132"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEN8434141.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27814..29196
                     /locus_tag="U8116_001133"
     CDS             27814..29196
                     /locus_tag="U8116_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN8434142.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            29323..29553
                     /locus_tag="U8116_001134"
     CDS             29323..29553
                     /locus_tag="U8116_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434143.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29753..30421)
                     /locus_tag="U8116_001135"
     CDS             complement(29753..30421)
                     /locus_tag="U8116_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEN8434144.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8610 a         6261 c         5566 g        10209 t
ORIGIN      
        1 tgcaatggta agttttctat ttttttgagt tttcgttcct taaaatcaag agattttaat
       61 tgtgtaataa ttacttgtcc ttttatatct agctcttcag gtaatgagta acgtgtcggt
      121 aaattcttta tagtggaagt aattggacat atgaccgaaa acattgtttt acggttaaat
      181 tcataacgag agacgactag tcctggtcgt cttttttgaa tttcttttcc tacggaggga
      241 tcaaaatcaa tccatacaat atcacctttt ttaggaatat aaattctttc ttctgacact
      301 ataaaatttc tctcccttca ggagttaaat cttcccattc gtctttctca taatactcgg
      361 cctcttcccc accactaaat ggatcttcaa tttttggaac taacgtaatt gttccatcat
      421 cagaatacac aacgatatat tcttggtttt ctgaaggttt ctttccgtta tcagaaggta
      481 aagttaccac tacagaactt ccttgtaatc tagattttgt aacaagcatt atcatcactc
      541 ccttaataaa agtatactat ataacactaa gtaacgcaaa attccccttt gtaaactatc
      601 tagaaaattg ttttgtttaa agtatatctt attggatata aatagaaata aattgtagcc
      661 agttcatttt ttctttaact aaaaaaataa tccaagaaac attgaaccta tggatccaaa
      721 agctacccaa atactatagc taaccccccg ctaccttcaa ttttgcagat aaaacgcttt
      781 gggagaaaat ttcaaagaca acaggccatc ctatcatgcc cgattataaa caaaaagctg
      841 gctattcctt ttgctctgac aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag
      901 aagtttcaac tgttcggatc aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa
      961 tttcaactag acaagacttt gctattgtag aacgtgagat cgtagctatt tttgtagaag
     1021 aagggatttt acatgatcca actcatatca atgtggataa atggcaacct cttatctata
     1081 aattcagaga atatacaact gccaaccagt cgttaacttt gaattttaga ttccaagaat
     1141 tcaaagagtc ataatgataa acatcgttag gtataaataa aaaagactcg cagcacgagt
     1201 aaaaaacaaa tagaagctat acattagaaa ggtcgactgt gaccctatag atgcagtata
     1261 tgcagcttat gagcatttct tctgttctct ttgtttttgg gatctgtgct gaagtctttt
     1321 tcgttttttg ccgctctttc tttgaaaaat cgattatgtt ataccttctc cattactaat
     1381 tcaggcatct gctgagtaat gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg
     1441 atttgttctt cagtcaagac aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta
     1501 ggttcagtag tcattatgat tgattctgcc attttttcat cctgatggct acgataaaaa
     1561 ccgccaatat acactatttt ttccttattt gaaaacataa atttgtcttt ttcttcattc
     1621 cactcattgg gaaaacacaa cgattttgca aaaatggttg atgaaaggtc tttttttctt
     1681 ctactgtctc tgcacgggca ttaatcatca attgtccttt tttaaatccg gtaaatcccc
     1741 acttcatgat ttccggagtg aggatgtttt cttcctcttt tcttaacgtc acaacaagat
     1801 ttgacggaaa aacttctcca gctgctaatt ctgctgcctg cttcggttgt ttcgtctggg
     1861 cttctcgata ataattctga aggagctctg actctagatc aaaaaaataa cgaccacaca
     1921 tcctaatccc tcccaatctt tttttctatc atacactgca acaagaagtt cgcaccaaca
     1981 aaacgctttc aatttttgta gaactaaaaa agttgtgaca agtgacaaac tctccagtga
     2041 atggtattct cctgccaact cctcggtcac aagtcacaat attcaacaaa catgagaagc
     2101 tgctttttga aattgttcct tgtgactcgg agtgaaacgg caggcttacg acctctgggt
     2161 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa
     2221 gctattttca caaaattttt cttaatactc gaaactaagc gactttttca caacctcttc
     2281 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg
     2341 agcagctatt tttatttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct
     2401 ctccactaaa cggtgttcaa tcagctaacc ttcggtatta gctcaaaaaa cggcaaaata
     2461 tgaggaacta ttttgccgtt tgtttctcta atactcaggt caaagcgctg atttcacaac
     2521 ctctaataca cggtgttcca gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa
     2581 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
     2641 aacctctaat tattaaagaa ccttttgcga agttaacaat ctttctttga tcaattgaaa
     2701 attctgccta agtagtgata gatcctgtgt gactttcaac aataagatac ttccctggat
     2761 ttcagcaatc aacgctttgg cttgacggtc tgcttcttca tcggacagtt tttcttcaga
     2821 aagtgccagg atattttgtt tcgtatactt gacccattga ttgactaatt ctagtagcaa
     2881 cagacggtat tcttcatcca ctgtactcaa ctcaataatc aaattaccta caggacagcc
     2941 atgataagtg atctgtgtct catgatcagc gaaaatccag tccaacattt cttctaatgc
     3001 ttcttctggc tgttttccag catttaagat gtcttcgaac aattctgtct gccatacgtc
     3061 catatgcgca tggatcaccg ctagacaaac ttcttttttt gaagaaaagt aataataaaa
     3121 ttgacctttt cctgtatcag aagctgctag aatatctcgg attgacgttt catgatagcc
     3181 ttttttataa ataagatctt tggtcgcttc caagatcttt tcctttgttt tcaatgatat
     3241 tctccttttt catttctcat agtcctatat tatacagtaa attcatcatt ttttcttctt
     3301 aatcgtttaa aaaagactgt gccaaattat ctgacacagc cctcactata aaactgttct
     3361 catatagcta gatgttcatt gattcaagaa ctgcgaggac aaaagcaatt cctatcttat
     3421 gatagttgct ctaatacttc ctctgttgtc atcacttgat ctgcaataaa gccaaagttc
     3481 gtcaacgctg catccattgc ttcttggcca ggagcaccaa ttgcatctgt cgctaccaca
     3541 acattgaatc cactctctac caattcacgc atatgggaat cgctacacaa gtttccgttc
     3601 attccaccaa gaattactgt gtcgattcca ttttttctca attggagcgc caaatcattg
     3661 ctttctggtc caaagatttt atgaggagag ctgatgatcg tattttcatc caaataaggt
     3721 ttcaattctt cgataaaatc ggctcctgaa ccttcttcca ctgattcata ttgacttttt
     3781 ctagcaaaca tctgctgctc aagcatcata tgttctcctg caccgccaaa ttgccattta
     3841 tggtcatgtg ggtaaaaata atgaggagaa ataaagagtt tgtatccttt ttcttttgct
     3901 tgttttacca tttcaatcat attttcgatc gtattgtatt tttctaagat atcttttgtc
     3961 aaaccaaagc caccgcctgc tggtttcaag aactcgactt gcgggtcagt gagaactact
     4021 gctgtatttt tattattgaa catggtggat tctccattcg ttatttattt taatacatca
     4081 tttagagctt cactcattgt cggatgagaa tagatttgat ctctcaataa cgtataagga
     4141 agttttccat tcatagctag cgaaatcaag ttgatgactt catgagaatc ttccgcatag
     4201 atacttgccc ctagaatcaa gtcagtttca gggtcgacta aaactttgaa tacaccttta
     4261 ggatcttcta atacttgtgc tttagggaca ccgcttgtca tatacttgaa taatttgtaa
     4321 ttgacgcctt gtgctttggc ttgtttttct gttaaaccta cattggataa agtcggagtg
     4381 ataaatacac tgtaaggcac agctttccgg ttattcgttg ttcgtttatt ctcgcccttc
     4441 aactgatcca taatgattcg ataatcatct agagagatat aagtgaactg caatccgcct
     4501 ttgacgtctc caatcgccca aatgttgtct gcatctgtac gaaggtattc atccacagcg
     4561 attgctccgc gatctgttag tttaacatct gtattttcta atccaagtga tgctgtactt
     4621 ggttttcttc cagttgcagc caaaactttt gacgcttgga ttgttacttc ttggccatct
     4681 tctgtaaagg tgacggcagc tgttgattct tggtctgcta cttgatctac agatacacct
     4741 agatggaatt cgattcccgc atttgtcatg tcttctaaaa tcatttgcgc gatatcttca
     4801 tcttcacgtg gaagaaattc agaatgggca tcaagaacga caactttaga tccgtattca
     4861 ttgaacattg atgcaaattc caagccaata tatccagccc cgatgatcac taatttttcc
     4921 ggtaattgtt cctgatccat cgcttgcgta gaatcaatca agtagcgact tgtattcaat
     4981 cctttgattg gtaaaatgat tggttcagaa ccagtattga tgaaaatccg ttcgccttca
     5041 acaatcactt tttcaccatt attcgtcact tcgatttgat ggttagaaag aaaacgtgct
     5101 gtaccgtcca tcactgtccc agttgcctca tcgctgatca tatgataatt tttggcgcga
     5161 agcatgcccg tcaatttttc tttccgagca acagcctctg taaagtcgat tcctctttct
     5221 ccattgaaaa tcaatgattt agaaggaata cagccgatgt tgatacatgt tccgccatac
     5281 atacgtgttg acttttcaac gactaaaacc gattcacctt ttgaagctaa cgccttcgct
     5341 aaagtcttac cgccctttcc aaaaccgatc acaatatttt gatatttttc catcattttt
     5401 ccctcgcttc ttttttacct accgattggt acaaaaatat gataaccgat aattgtctat
     5461 tctccaaatc atctgcttta tttgtcaaaa aaaaccagac aaaaagcggt cctttcacgt
     5521 ttttagtctg gttcgatttc ctttattctt ctaaacaatc gttacttcta catcgatatt
     5581 tcctctagtt gctttggagt aagggcagaa ttcatgcgca tcttctgcaa ttttttgggc
     5641 ttcttcttcg ctgatgccat cgatatgcac ttctaatact acaccgatct gatagccgcc
     5701 atcatctaaa ttataaagag agacacgtgc ttttactgtg gactctgctg tcattcctgc
     5761 ttctttgatc atatgttgta ccgccccatt gaaacaagaa gcataacctg ctgcaaataa
     5821 ttgttctgga tttgttcctt ctgcatggat tcctggagga gtgattttca tatgcatcga
     5881 gccatttggt gcctcgactt ctccattgcg cccgccatgg ttgatcattg ctgtttcgta
     5941 tgcttttttc attgtactgc tcctttcgat tgaccatttg ctacttggct ataattatag
     6001 tgtatgacaa gtaaggcgct ttctacaaaa attctgctta ttcgaatacc agaatgccgt
     6061 cttctactat ttttccaaaa aacaaaaaaa tactactcgt gaacaacagc gtcatatttg
     6121 ttccaaaata aatagcaatc ttttctacat aatcttttat atgattgatt ttaagataca
     6181 tatataccag aaagttgacc ccataaacca atactgttaa aaaaataacc atattcgtta
     6241 ccattttttc ctccttttgc tggtctccct accagcactc gatttaacca taccactttt
     6301 tttgccgtcc aagcaactag cctttcaaaa agactggttt attctgctgt taatgattca
     6361 agatcagaaa aatgtccgaa taattttgct aattttacta acgccaactt gttctttccg
     6421 aattcatcat tttctatctg taatactagt aacggctcat gtaagcttaa acggatcaaa
     6481 aaccagccgt ttccgtatgg ctcttctaga tcatatcgaa taccttcttc attttccggg
     6541 tcgaagttca tccctttttc ttcttccaac agtcgcttca tttcttctaa tatcttgttt
     6601 ccttgttcgc gtggttcatc gctgaacaat tttaatcgga tttcttgtac ttctacgggc
     6661 tgtttcaact ctttgatttg ttcatctaat gtgatacctt ttctttttaa ttctggcaaa
     6721 agcatcagga tctttgcgat gacatatgca ccatcatcca gaaaatagtt ttccttgaat
     6781 gcagcgtgtc cgcttgtttc gatggctagc tgtgtatctt tcccaagacg gttaagttct
     6841 aatgctttat tgattacatt tcgatagccg gaaatataac gaacttggat gcctcctttt
     6901 tcttcaatga atctttgtag atgagaagaa gtaggggaat tcgtcacgat tcttgttcct
     6961 ggatgctctt ttaatacgat tgtacttaat actgcgatca aattatttcg attgatcact
     7021 gttccagaag cactgacaac tgcagaacga tccacatctg tatcgaaaat gatcccaaga
     7081 tctgcttcat ttgctaaaac agcctgcttg atactttcca tcgcttcttt attatcagga
     7141 tttgggatat gattaggaaa tcggccatct ggttccaaaa attgggaacc tgttgaatcc
     7201 gcacctaagc tattcaatac tttttcagca aagaatccac cagcaccatt tccggcatca
     7261 acaatgattt tccaaccatt tagtggtttg ctttctagtg ttccaatccc ctttttaatt
     7321 tttgtgacta aatcttgcgc ataaggtgtc aaaaggtctg ctttttctac gcttccagtt
     7381 tgttcgcttt ttattttttg ctgctgtgat aggataaaag caatatcttc tttttctgct
     7441 cctccttctg aggtaaagaa ttttattcca ttataataat agggaagatg gctagcagtc
     7501 agcataaccg cagcatcgca cgagaactgt tcaaattgag tggacataaa catagcaggc
     7561 gtagtagcta attcgaaatc gatgacatgt atcccttgtt ctttcagact ttggatcaac
     7621 gcctctttca gtgctggtcc gcttatccta ctgtcatgac caatcccaat cgtccatttt
     7681 ttatttggat cggttgattt cttctttttc tttatccagt ttgctagtcc ccagccgatc
     7741 ttttttacca tgtctgtcgt caagtttact tcatatcctt caactggtaa tgcgattcct
     7801 cgaatatccg atccattttg cagcgctttc atatctttca tataaaagtc ctcctcattt
     7861 attccctcat tttttccagc ttttctgcaa atcgctgtgc ttcttcttct gagcggcaaa
     7921 taataatgaa tgtatctaca ccggccatcg ttccgacaac ttcagggtaa gccacttcat
     7981 ctagccaatt cgttaccaca tcggcaccat ttcgttcagt caatacgata accatgaact
     8041 gaacagactg gatacgcaaa acctcgcgtc taattgctgt acgcaaacgt tcttcattta
     8101 aagaaacaga tggctggtta aataatgtat agcgtactat tttgttttca ttttgacttt
     8161 ttactaactt catttctcga atgtctcgag aaatcgttgc ttgagtcaca tcggcgcctg
     8221 cttcatgcag ccgcgcaagc agctcttcct gtgtcccaat aggttgttct gtaatgatct
     8281 ggcggatcag tgcttgtcgc tgtttctttt tcatattaga cacctctcaa tgttgattat
     8341 agcaaaagat aagtaatatt tcttcatatt ttggtatttt ttacttttat tttttgtttt
     8401 cattcaggtt gctaaccttt ttctatttta acacgcaaaa tcgtcttcat tttttcaggt
     8461 gctgattttc ttggatcgct gatgtaaatc tctttgtgtt ctttagaagc tctttcatag
     8521 ccttcttttg cgatgaatgc ttctaatttc tcaaacgttg ctggttcttc ggcatagggc
     8581 ccgacgtgta agatttgggc acaaagacct tcttccatct ttttgaatac tacttgttct
     8641 ttcaagtctt ccggaagttt gctagaacgc tgaatggctt cttgtgctat cgcttctgta
     8701 acaaagtcag gctgtcggat catcaattga taagaaaaat gttctttttt catcactggt
     8761 tcatgcaaat acttttcttg cagtccccat tgtccttcca gtggatagac agtatacatc
     8821 tgataatcag gaatcagcca atttttttta ggcgacatgc gtattgcata gctgactgca
     8881 tatagacagg ctactcttcg tttaaagtct tcctgattcg ggtcgccgat ccctctgatg
     8941 ctaaagaatg attttttagg aacctctagt atttgcgggc tttttttcat actatagctc
     9001 ttgtctttac gccattcaat tttttccatt tttcttcctc cgcgaaattc catttagtta
     9061 tttattatca tacctctttt tgagtgactt ggctaatcgt gttgacacta tttttcagtt
     9121 tgtgctagaa taagtgaagt aaataaatcg tacagataac acgtttcggc gtgtcgagag
     9181 agaggcggtt tctattttta taatagaaat cactctcttt ttttacgaaa ttttttagag
     9241 gtgatgtaaa tgacaaaaca aaaacagctg cttttagtac ctgcagtaat tgcaacaggg
     9301 atgatgtctt ttgcgggcgt attgatcgag acggccatga atgtaacttt cccaactctt
     9361 atcagacagt ttgatttaac gactgcacaa gtccaatggg taacaacgat ttatcttttg
     9421 atgatttcga tcattgttcc gctgtctact tatttgaatc gcaattttag tctcaaaaaa
     9481 ctctttttag cttcaaatct gcttttcttg gcaggtataa tgatcgattt cttctctcct
     9541 accttcatgg tcctattgtt tggtcgattg ctccaaggtg cagcgacagg tatcgctctt
     9601 ccgttgatgt tccatattat cttgacattt gcccccctca atcgacgagg cgcgatgatg
     9661 ggtgtcggta ctttgacaac ggccattgct ccagcaattg gccccactta tggaggcatt
     9721 atgacttcac attttacatg gaatcatatc ttcctgtttc ttgtgcccgt tttgtttctt
     9781 tctttgatta tcggctggtt tgctattccc gagatggcag tacaaaaaag tggttatttg
     9841 gattggataa gcgttttagg aatttgtctt ttgtttagtg gaagtctaac atttttgagt
     9901 acgttgggaa cagtttccag ctggattgct ttaatcatcg gcggtattgg ctggttcctt
     9961 ttttactatc gaagtaaaaa agtggagcat ccactgatcc gactgtctat tctttcaaat
    10021 aaaacattcc gcttgttttt gattggcttc ttagtttatc agtttttatt acttggtgta
    10081 tcttttgtct tgccgaattt ccttcagatc gttcaaggaa gtagtccttt tgtcgctgga
    10141 ttagcgatgc ttcccggcgc agcggttggt gctttgcttt ctcctttttc cgggaaaatg
    10201 ctggatcaat atggtccgaa gaagccaatt tttgctgggc tgattctttc tttgattggt
    10261 tggctagcat tattcctact cataggacac gcttcagtgg gtgtattgat tgcttgtcat
    10321 gtgttttata tgatcggtat cggattatct tacagcaata tgatgaccac cggaatgaat
    10381 gcactgaatg aagaactaca aggagacggg aacgcaatgt tcaatacctt gcagcagttt
    10441 tctggcgctg tggctacttc attagtagcg gtcattatca attacgtcca gcatcatact
    10501 tcccataact atgaagtgtc tactacatta ggatcaaaag cagcattagg cgtcttgttg
    10561 ctgttgcttc tcgtaagctt tgctcgattc gcctattatc tgttctttgc taaaaaagcg
    10621 tagattccat atacggcagg tgttgcatct gccgtttttt tgtgtgaaaa tggttgtttg
    10681 taaaaggagg cttttttatg gaaaaaattg ccattatcgg tggcgggatc attggtatga
    10741 ccctagccag ttacttagat acatcaaaat tcaatgttgc tttatttgat gaagggacag
    10801 gacaggcaac taaagctagc gctgggatta tctccccttg gttgtcgaaa cgcagaaaca
    10861 aacaatggta tcgtctggct aaagacggcg ctgctttttt ccctaaactt ataaaagatt
    10921 tccaacttga tcaaacgatt tacgcacaat cagggacact tttgcttcga cctgaaaaag
    10981 atttagctga attggctgtt cttgccgaga aacgaaaaga agaagcgcca gaaattggaa
    11041 acatcgaatg gctttcacct gaacagacat ctcaagcact tccgttattg aaaccagcag
    11101 tttcactgcg tatcagtggt ggtggtcgtc tggatggaaa agcatattta gaacatcttg
    11161 gtcagcgttt agcacaaaaa caaatggagc agatccaggc aaaagtcact ttgcataaga
    11221 agagcgattc ttggctcatc aagggaccgt ttggagaata cacagctgat cgtgtaattt
    11281 tgacacctgg cccggcactt aagcctttat tacaagaatt aggctatgct gcagatatac
    11341 gtccgcaaaa agggcagctc ttagcatttc agacatctta taagatgagt caagactggc
    11401 ctgttgctat gcttgacgga gaagctgatt tcattccttt catggatgga aagatccttt
    11461 taggtgctac ccatgaaaac gatcaaggct gggatttggc tcctactagc gaagcttata
    11521 agggattgac taagagtaca gaacagttct tgactgatcc agatcagata aaaaactggc
    11581 catacgagtt ccgtgtaggt acccgtgctt atacgtcaga tttttcccca tttttcggcg
    11641 tactgccaga tgatccaagc ttactggctg ctagcggtct aggctcttct ggcttgacca
    11701 caggtcctta tatcggctat ctattagcca acttcttaaa tactggtcat tggcctacgg
    11761 aagattatca aaaggacatc tccacctata ttacgaaaaa tccatcatag aaaaacagct
    11821 ttttcaagta aggatcccga gcgatattgc gcatggatta cttcttgaaa aagctgttat
    11881 tttagttcaa tttctgaatc aaattctcca aactttcttt tgcttcatct ggcaagagat
    11941 cttttcctat ctgagcttca atgaatcgta aacgaatatc aagtcgttgt ttcaccagat
    12001 ttttatagga cgtatcctca aaagaaacag gaaaatctgg tataggtaaa tagtggatgt
    12061 cttcaaatgg accaaacgac ttgaattctg ccgtttgttg cacgattctt tcaacgattg
    12121 acaatgtcgt ttcaggtttg atttttttat catcgaaata tccagtattc aggacatagc
    12181 aggctgtttg ctgtttcaaa aacaactctc gaaaagcttg atagtcttcc ctaagaggat
    12241 agacacggaa aggattagca aatggttcaa tgactagctc atctagctgt ctttttgtag
    12301 tgatattttc cgcgtctgat cgttttgttg ctaatgttgc accaaaagac gctgctaaaa
    12361 ctgggtcatt gatcttgata attggcggaa gcgaatcatc tttcatgatc caaaaaacag
    12421 catcgatctt ttcgctaaga tgatcgactc ggtcgggtgt cgcataacgt gatttgactg
    12481 tccgcccatt tccgtttcgt atatctcctg ttactaggac tttttctcct ttacttgtac
    12541 atgtcacgcc cacattctga caagtaagga aagaagaaat ggccggatca tcagacggat
    12601 agtcttgtgt tttatcaaag taagaaggtt ccaaggcagt agcaaaacca ttttttcgag
    12661 aaataacgaa agcatcgtca tgcaagatcg aaacagagtg accttccgta tgttttgcta
    12721 aggtaatcgt tgattttcca gaaccggaca acccaaatac agccatcgta taggtctggt
    12781 cattactagc atattgtttc atcccaccgt ggcaggcaat aaagccattg cgatttccca
    12841 atgcccaagc aagcgtcaaa gtcgcttttt ttaattctcc aaagtaacgt aatcctagca
    12901 cagccgctgt attatggaga gcatcaaaca aaatgagtcc atttggaaat gtttcatgtg
    12961 tccattcggg atcacaaaag atataaagat ctccctcatc aaaggaaaca gacttttgat
    13021 aagtctctaa gatcttcgaa ttcgctggct gaaaattcag caaataagaa tacaaattgt
    13081 tctcaaatcc ttctggaatc agcaaatgac tttgcacaga aaattcttca gataggccaa
    13141 cgattacttt tgtatggtaa aactggcgct tgcgactttg atacacagct tctcttaaaa
    13201 ttttctcgta tttgctttgt tccttgtcta agtcctcaaa gatccgccga gcacttgctg
    13261 ttcttcccac aatgcttcca tcattgaata ctagtgtcct aacatcagct ggtaaatcta
    13321 gttcttcagc atgtgagatt gttaaatctg tgatgatcgt tcctggactc ccttttgcta
    13381 gttgataagc tgtcttccta tcaaaaactt catgaacatt attaccgtaa aatgtcgttt
    13441 ctacaatcga tcgaatactg gataacagtg gatttccact tgtgatttcg tttaattcga
    13501 gactctcttt tgtactcatg gctcctcctc attccctaaa caaaaaaacg ttttctttat
    13561 tatacattat tcgagttttt atttgtactg ctgcgctgtg ggaagcaaac gagttatttt
    13621 tcacacaaaa aaaccgagaa cttgaataaa caagtttctc ggtttcatca ttttcctttt
    13681 atcggcttag taccagccgt ttgctaacca gaatgtttta gcagcatccc atgaaccata
    13741 acgagatgtt acatattgtt ctgctacacg ttcttgattg gcagctgaat agtcgccgtt
    13801 taagtatgaa gcatctaatt gataacggcc gatgtaacgt ccgttagtag ctgtgtatga
    13861 accgcctgat tctttttgtg cgatccattc ttttgcagag cttgaagttg gcgctgttgc
    13921 agcagctggg gcagcaggag ctacttcaac tgtttcaaca ggtgcaacgt atgtatgttc
    13981 ttgcgttgct gtttcagttg tagatgctgt attttctgtt gttttgtttt ctgttgttgc
    14041 tgaagaatca tttgaagttg ggattgttaa ttgttcacca acataaatgc ggttgatgtt
    14101 tgcgatcgaa tttttttcag caattgcatc gatcattgag ttgtctcctg caaatttttg
    14161 tgagatctta gataatgaat cacctgattt tactgtataa acttcatcag cgtgagcatt
    14221 tgtagttcct acaaacattg cagtagcacc agcagcaaaa gttgtaccta aaacaatttt
    14281 cttaaatgaa ttcatattaa actccttttt ttctccgttt tttattcttt cgacgctatt
    14341 aactttatca tttagaaata tttcataggt aaaagaaaca tgacatttaa tgacaaaata
    14401 acgaattatt taatatttgt aacatttaaa caaatatttt tttgatacaa aaccataaaa
    14461 gccttgttgt ataagcaaag taatcgtttt tctatgtttt tttatttttg aatgaaaaac
    14521 gcagattctg taacattcac tgcaataatc cataaaaaaa agcaaaaaaa tttttgattt
    14581 ttatcaaatt ctcttaaaaa aatgtaaaaa atacaaaaaa accagtaatt gttacaacaa
    14641 ttactggaca aaaactttga ataatgttaa ctattttttt attttaactt tctccgcaat
    14701 aatttctggg catgttccaa ttcgtttata acgacttctt ctgtttcttt agctaatatt
    14761 tcatctaata catcaagcca tttttccggt tcttttgttc ctagacgtcc aatagcccaa
    14821 gctgctgttc ctctgatgac agggcggaca tctgttaaac attctgaaat ctcaggcaat
    14881 gccgttcgat cacctagatt agctaaagca atcaatgcgt ttctctgcaa aggtttcttc
    14941 ccacgccatg aaccagctaa atgcccaaat tgctgtttga agtctttatt agacatcgaa
    15001 agcattggct ttaattttgg atatatttcg tctacttttg gttccatctc ttcatgaaaa
    15061 tgaaaatctt ttccttgatt gtaagggcag actaactggc agatatcaca tccataaatg
    15121 acatttcgca tttttttacg atattcttcc ggcatcatcc cttttgtctg agtttgataa
    15181 gacaaacatc gctgggcatt cattcttccg tcaccaagca atgcttttgt cggacaagca
    15241 gtcacgcagc gcatacagtc accgcaacca aattctcctg gtttatcagg ctcaaataca
    15301 atattagtaa cgatctcacc aagatagaca aatgaaccga actcttcagt gatcaacaat
    15361 ccgtttcgtc caataaatcc taaaccagct cgctgtgcta cagcgacatc gatcagttct
    15421 ccagtatcta cttgcggagc tagacgccat tcctcttctt tttgccattt ttctgctctt
    15481 gtttggatga attcgatcaa tcggttcaaa cggtctctga gtatatcatg gtaatcgatc
    15541 ccccaagaag cacgagcaaa ctgcccgcgt ttttcatccc taggcacttc ttcatggatc
    15601 ctagtaggat acgcaagtgc aatggaaatg atcgatttcg gctgatcaaa tgtcatttct
    15661 ggataaagac gctcatcgat atttggatgt tcaaagccag aagtatggcc agctgctttt
    15721 tgttcttcca acgattcttt taatgaatca aaaggttccg ccgtggtgaa cccgattttg
    15781 tcaattccta atcgtttgct ttcttgtatg atttcttctt ttaatgtcat ggtcttcctc
    15841 gcttgctgtt aacggctgta taacccataa tatagttttt cttttttcag ctggacaaac
    15901 tggatctgct gatcggcaat cttcttttct tccatccatt tttttcttgc agcaaatact
    15961 tcccaataat ttgttgaaat taattttctt ttgttatcta atgcattaaa aaaattcaat
    16021 gaaactggtt ttgtacttaa cagttcatgg agccttacct cttcccatgg cgaaaactca
    16081 gacagatcgc gggaatacaa tacttcttca tagagatttt tttgctgtgt gtacgtgaat
    16141 gtttgcggga ttttcccagg cctagcaatt tctactagac gattataaaa catattcatt
    16201 aatttgctgt tggcaataaa ttgactttta taataggccg actggatctt tttgattact
    16261 ccttctgctt tgatgactga aaataatgca ggccatgctt tgacggttat ttcgtcgttt
    16321 tgtttaacta atacgccagg tgtgaagccg actggagaaa ggaacacaaa ataaccgata
    16381 ccggtatttt catctaaagg ctgaaattca tcaaacaacc atttgcctat gtacagctct
    16441 tcatctggtt ctgttgtctc attttgattt gctatgaata atttagctaa ttctgcttca
    16501 agaaattgaa ggtcttgatc cttgtcggta ttttcctgct ggctgatcaa tcgaaagact
    16561 tcttctttaa tcgtatattt atctttacgt gtaaaattca tagccacacc ttccttcctt
    16621 actctaacta taacggaatt tcttcaaaaa aggtatctgt acgacgaaaa acagcataat
    16681 tttttttcat atctcttata aaaaatgcaa ggtctgctcc tcaagctaga ccttgcactt
    16741 ttcagctttc ttttgtttgt gacaaacctt catggatcgt ttcaatattc catttcatca
    16801 tctgatagta agaatctcct tgttctcctt cttttgctat ggaatcagtg aacagtttat
    16861 catagatttt caaacccgtt tcttttgcta cccgttccat gcttcttgag tctacactgg
    16921 tttccacgaa taaaaccggt acttcttttg ctcttatctg atcaatgatc gctttcattt
    16981 gatcaggcgt accttgactt tctgtattga tctcccatat ataagctgct ggcaaatcat
    17041 atgctcgtga aaaatacttg aatgcccctt cacttgttac taataatttt ttgttctctt
    17101 cgatagaagc aaaactttcc ttcgcttgtg tatctaattc ttttagtttt tccacatatg
    17161 cttctgcatt ttttttatag agtgtcgcat tttctgcatc tttttcagag aatatacgtg
    17221 cgatttcctc cacatatttt attccgttag ataggtctag ccaagcatgg ggatctgctt
    17281 ttgtatgttc ttcaccgcta gttaaataaa gaggttctac atttttgcta actgcaaaat
    17341 aatcttttcc ttcttctttt ttagccgttt ccatcaagtt atcgaaccag ctgttacctg
    17401 tttcaagatt caaaccgttg tataaaataa catctgcatc gcttgccttt ttgatgtctt
    17461 ctggtagtac ttcatattca tgcggatctg ttccgactgg tacgatactg tggatatcta
    17521 tttgatctgt tccgacctct tttgccatgt ccgccaagat cgaattggta gccacgactg
    17581 acaacttttc tttacttcct tcatccgctt ggtcagaagc ctgctttccg caaccggcta
    17641 ataccaagaa actaactgcc aagattaaaa agagtgattt ttttgttttc atctatctgc
    17701 ttcctttctg aaataaaatt ccttttgttg gagaaaataa aaaagcaagg ataaaaaatg
    17761 cggtacatgc taacacgatc gtagctcctg atggccaatt gtaactataa ctgaaaaaca
    17821 caccgacaac tgcacttagc ataccgattc ctgcagctgt aatgatcatt ttcagtaaat
    17881 ggttcgttag caagtaagcc gtggcggctg gtgtgatcaa catggcaatc accaagattg
    17941 ttcctactgt ctgtaaactg actacagcca ctaatgtcaa aaagaacatc aaaagataat
    18001 ggatcagcga tgtgttcagc ccataagctt ttgccattgt cggatcgaat gatgtgatct
    18061 tcaattcttt ataaaagaaa aagacaaata tcaagacgat tccactaacg agtgctgtca
    18121 gcaacaaatc gctttcccta acagctaaga cattcccaaa taaaatatga tacaaatctg
    18181 tcgcactctg ggcatatgaa atcaagataa tccctaatgc aaaaaaggag ctaaagacaa
    18241 ttccaatcgc tgtatcattt ttcaatcggc tccgctgagt cacgaaacca atcactgctg
    18301 ctgttagcat cccaaaagca gtggctccaa agatgtaact aaaaccaaac atataagaaa
    18361 tggctacacc tggaagaact gcgtgagaga tagcgtctcc catcaaagac atccctcgta
    18421 aaataataaa agaaccgatg actccagaag caagtcctac aagcatggaa gtgaccaaag
    18481 cgtattgcag gaattgataa ttcattaatc catcaataaa ggattggatc attttttctc
    18541 tcctcctttt actaaatgtc tgatgatctc accataggcc aattgaaggt tcttcgatgt
    18601 aaagacttct tgtactggac cggaagcgat cagctgttta tttaaaagaa taacctcatc
    18661 gaaatattct tctactttat gaagatcatg atggacgatc aaaatcgttt ttcctgaatt
    18721 cttcaattcc tgcaagatgt caaagatctt tcgttcactt aacgcatcaa tccctacgaa
    18781 tggttcatct aaaaagatcc attctgctcc ttgggctaga gctcttgcaa taaaaactct
    18841 ctgaagctgt ccacccgata gttcgctgat ctgtcttttg gcatattctt ccatccctac
    18901 ttttttcaaa gcttgtcttg cacgttcttt ttcttttttc ccaggtcttt gtccaattcg
    18961 taaagatgga tatgttccta aaagtacaac gcctaataca tcaattggaa aggaaagatc
    19021 caattcactt cgctgttcca cgtaggctat ttttttacgg actgagttta ccgactgtcc
    19081 agaaaaggtg actttaccag tagtagctgg tatcaactct agcaaagatt tcataaatgt
    19141 tgattttcct gcaccgtttg gtccgataat accggtaatt ttttgtggat ttatcgtcaa
    19201 gttgatagtt ttcaaaacag ttttcccttg ataagcggct gaaaggtctt gaattacaat
    19261 ggccgtacgt tccaaatctt acactccttt ttaggtttac ctaacttttt atttaaatat
    19321 agtttaacta aaatggaaag cattttcaag tagaatgatg aaaaagtttt tttcgaattt
    19381 tcctcgcatt ttatgaaaat tttctgtaga atcacatcaa gagttcaata aggctaacct
    19441 aataatgaaa aacatgtgtt gaaacgtttg attatttttt cttgttgact caacaacgaa
    19501 aggagttttt gtatggaaga agaaaagtta actcgaagtc ttagtgcccg tcatatccaa
    19561 atgatcgcac taggtggaac gatcggtgtc ggtctgttta tgggtgcctc ctcaaccata
    19621 cgttggacag ggccctcagt gatgggagcc tatgcgattg caggattctt tttgtatttg
    19681 attatgcgtg ctttaggcga aatgctttac gttgatcctt cgactggttc ttttgcaaat
    19741 tacgcgagcg agtacatcca tcctgtggct ggctacttga ccgcttggag taatattttt
    19801 caatatattg tcgtaggaat aagtgaagtg attgctgtag gatcttatat gaattattgg
    19861 tggcctgatt tgccggcaat tattcccgga atcattgtcg tcttgtttct tatgctggca
    19921 aatcttattt ctgtcaaggc gtttggcgaa ttagaatttt ggttttcgat catcaaagtc
    19981 ataacgatca ttttgatgat cattgcaggt cttggggtaa tcttatttgg atttggtaat
    20041 catggtcagg cagtagggat ttcaaatctt tggaaaaatg gtggattctt cactggaggc
    20101 gtcaaaggat ttttctttgc attatcgatc gttgttgctt cctatcaagg aatcgaactg
    20161 atcggaatga ctgctggtga agcagagaat cctaaaaaga cgatcattga agctgtccag
    20221 tccactatcg gtcgaatctt gattttttat attggtgcta tttttatcat tgtgagtatc
    20281 tatccgtgga atcaattgag tgagattggt tcaccttttg tacaaacttt ttctaaagtc
    20341 ggcattacat ttgctgcagg actgattaat tttgtcgtga ttacagcggc attatcaggt
    20401 tgcaattcag ggattttcag cgcaagtcgt atggtctata cacttgccat aaatggaaag
    20461 atgtctaaaa aatttctgaa actatctcgt cacggtgcgc ctttttatcc tgtcgtagca
    20521 atctcaggtg gaatcttaat tggtctgatc ttaaattatc ttctaccaat gttcatcaac
    20581 cagtcagaaa atattttcgt tttcgtatac agctcaagta ttttgcctgg tatggttcct
    20641 tgggtagtga ttttgatcag cgagattcgc ttcaaaaaga agcacgcaga taaaatgcat
    20701 gatcatcctt ttaaaatgcc actatctcct tatacgaatt atgtgacact tgctttttta
    20761 gctatcgtat tgattttcat gtttatcaat cctgaaacga gaatctcatt gattattgga
    20821 ctcgttttct tgctttacat gacgatccat tactttgtac gcgaaaaaag aaccggaaaa
    20881 taaaaaaaga acgaagcttc accttagtgg aagcttcgtt cttttttatt gcttttatca
    20941 attcaatttt agcgtctcac cgggagtcag gaaataattc tccatgctca tcccattcag
    21001 cgacaagatc gtttctactg gtacgccagt tcgttcggct atttgatttg gaccttctcc
    21061 ttccaaaact acggttgtcg cagcctgtga agtcgcgctg ttggtattag tttcagagga
    21121 atctgttgtg tcttgtgttg ttcgagcttg catttcagaa gcttctaccg actctgctgt
    21181 ctcagaatca gaagatgttg tgtcttgtgt gcttgttgac tctgcttgaa gagaggcgct
    21241 agtctgcaaa taaatatcgg aaatcttagc gacatgattc gtataattag ccgataattg
    21301 attgatcaaa gtatctaagt ctacaccgtc tttattttct gctgcctgat acgctgcacg
    21361 taattttctg atcccttcgt cggagattgc ttgaagctca cgtgcttttt ggttagcaac
    21421 tgctgataaa ccagctattc cctcttgatt tccctgaatt tctgtctggt attcttcaat
    21481 caatgcaggc gtcttttctt cgatcttttt cgaataatca tttacgattg cttgaagttg
    21541 ctctggcaaa ccatttgctg ttttttcttc agaagtttgt gtctcttttg ttttagttga
    21601 tgactcactt gtttgtttcg tctctgtttt ctttgtcgtt gtttccgtta tggtcgtact
    21661 tttcgcttct gacgaatttt ttccatgaaa atatagaccc gcaactcctc ctgctatgat
    21721 caaaaacacg gcaactgctg ccccaatcat gatcatccgc ttacgagctg cctgtttttg
    21781 ctgtcttctt cgggtcatcc gaacattttc tccccactca ttttcatcat acattttttt
    21841 caccaccaat ttttatgtat aatagcgtta tcaaacgctt catattctta ttgtagcaaa
    21901 taattgtcgc tgtgcacatt tttatctttg ttatttataa gaaaatttca ccagttgaga
    21961 tgattccaag attgtccgtc gatttctcct tgcagctgca ttgttcgttc gtctatctca
    22021 gccatcagtt tctttatttc atataagagc gcacggtctt gatattctga tttttcgctt
    22081 aattcatcta attgttggat cagccaatca tttgtcataa tgaagttcct ttcatttctt
    22141 gcagtgtaaa ggtcaagtct tcgatctgct cttttgcctc agtcagtgct tctacgatat
    22201 atgcttgttt ttcagattct tcttcctgca ttttctgatt ttcctgtact ctttgaataa
    22261 accagtcaat ccgctgagtc agcgatggtt tgccttccaa tgagagatac tgctgataag
    22321 caattagctt ctcccaacgt ttttcctggg ataaatacat aaattgtgct aaaacgaatg
    22381 gcggaagata ggtcatatgg aatttatttg cgattcgctg atacggcctc aaaaattcgg
    22441 aaatcgtata ttcttctttt cctccagctt ggtattcttt ttccgccaca cctgttgtca
    22501 atacgattcc tagttctttc ccttttaata catgtgctgc tcgttccaag acattatctt
    22561 gccaaatttt taaatgagca ggagaactat accagtaaaa cggaaactgg aaaatgatcc
    22621 gatcatgctg ggccaagagc tgtagttcag tctctatatc tatttttccg tctggataac
    22681 ttgattccaa atgatgccaa gtagctggta aaccagcaga agcttctttg aaaaattgct
    22741 gatttaaaga ttcttcgatc gttggatggc taatgatgat aagtgtacgc atcccttttc
    22801 ctccttttta gtcttcgctt tacagtgtag cataaccgcc acaagtttct tttgccgatg
    22861 cttgtcattt taaccagact aatcaggttg tccaagaaac tgcggtttgt ttttccaact
    22921 gttttttcaa ggtatccaaa gcaatattct taaaattttc gtttttcata cgtttacgat
    22981 agttcgtagc cgtttctccg gtactctttt tgaacattcg gctaaatacg actagactat
    23041 tgaaccctac ttgataggca atactttcca cggattcatc cgtcagagaa agaagaaacg
    23101 cccctcgatt gatccgtacc ccgatcaaat attccttgat actggtaccc atatgatttt
    23161 tgaatactcg cgacaaatgg ctccgattga ccgctaaatg ctgggcgatt tcctcaacag
    23221 acaatttttc agcataatgt gcctcgatgt atttgactgt ttcaacagct aaagaggaat
    23281 gaaccttttg tacaccaccg ctgacctttc cgccatcttt taaaaggact gataaaaaac
    23341 ggtaggttaa tgctgttaac tgtaattcat tttccaaggt ttcttccgta tctttcatgc
    23401 attctaaaat aatgtcgata tatttttgta tgtcagaaga gaccatcgtg tattggtctt
    23461 ctctgaaaag cgactgccga atgatttctt ctgcagcact cccaccaaat gaaagccagc
    23521 aatacatcca cggatcttct ccatctgata agtaaaacgt cgagtctcct ggacgaatca
    23581 agaacagatc accttttttc aattggaatt gttgattttt cacatgataa gatcctttgc
    23641 cttccatcac gacatgcagg atgtaatcat tgcgaatagc aggaccaaaa gaatgaaacg
    23701 gcatcgtttt agagaaacca caaaaatcaa aatacaattc tttttctact tgttgatgtt
    23761 ccttttttaa aaaaatcgat tgctccacgt ctatccctcc ttactccttt agtataatgc
    23821 aacaaaaagt taaataaaag tcacagaaat atattaaaaa ggaagcgatt tcttgttaag
    23881 tttaagtcat taaaagatct ttgttattcg ttgtaaccgg tttacttagt attatatatt
    23941 gaacaggaga ggaatacaga ttatgaaaag caaaatgatc caacgtgtgt cctacgcttt
    24001 aggcgctttt ggacacgaca cattctatgt tacattaagc acttatttta tggtattcgt
    24061 gacaagccag atgttcgcag gaacagataa ggcaacgaat gcaaaaatga ttgctacagt
    24121 tacaagctta gttgtaggta tccgcttagt tgaaattatt tttgatcctt tgattggtgg
    24181 catcatcgat aatactcgta caaaatacgg taaattcaaa ccatggttag ttatcggcgg
    24241 cgttgttagt tccgtactct tggtagcaat ctttaccaat ttctttggct tagctactca
    24301 taatacgact ttatttatca tattatttgc aatcgttttc atcttattgg atagttttta
    24361 ttcatttaag gatatcgctt tttggtcaat gattccagct ttgagtacgg aaacaaagga
    24421 acgtgaaaaa tttgctacgt ttgctcgttt cggctcttct ctaggtgcaa atggtactac
    24481 tctagtcgtt gtccctatcg ttactttctt tacctatctt gttactcata aacatattga
    24541 aggcgcttca ggctggttct ggttcggtgt gattgctgct gttatttcag gcggtactgc
    24601 gattatcact gccttgggca caaaagaagt cgatactgcg atccgtcagc aaaatgaaaa
    24661 agctgggatc aaagatgtgt tcaaagcagt tgctcaaaat gatcagttga tgtggcttgc
    24721 ccttgcttat atcgcttatg cgattgccaa tgcagcaaca actgccgtat tattctactt
    24781 cttcaaatat gtacttggcc aaccaactga attttggatc gttggtctag ttgctgcctt
    24841 acttggttta gtagctgttc cactattccc aatgcttaca cgcgtcatct ctcgtcgcta
    24901 cgtttacctt ggagggatcg caatgatgat ttcttcttac tttttcttta cgatcgcagg
    24961 ttcaagtttg ccaatcgtta tcatcgggct agtattgttc tacttccctc aacaattgat
    25021 tttcttatcc gctttgatga cgatcacaga ctctgttgaa tatggtcaat ggaaaaatgg
    25081 tatccgtaac gaagctgtta ctctatcaat tcgcccttta ctagataaaa tcgctggtgc
    25141 tttgtctaac gggatcgtag ggtttgtcgc tatcgcttct gggatgactg gtaatgctac
    25201 tgctgctgat atcactgctc atggcgtaac gactttcaaa gcttatgcat tctatgctcc
    25261 tgctgctttg atgattttct ccgctattat ttttgcttgg aagattacat tgacagagaa
    25321 aaaacatgca caaatcgttg agaaattgga aaacaaattg gctccagctg atacaatgga
    25381 agatgaactt cacgatgcag tagatccttc ccattaagaa aggatcttga aaatgaagat
    25441 tacagaaaaa gattttggcc aaggttatca cttgataacc ttggagaaca aaaacaagct
    25501 agcactttct atctctgatt taggtgctag aatcgtcagt ttaaaatcaa atgatcgaga
    25561 attagttttg ggatttgaca ccgctgaaga atatatcgaa aaagatccgt atatcggtgc
    25621 ttctatcggt cgcaccgcag ggagaatcga aaatggccgc ttttctctca atggaaaaac
    25681 ttaccaatta gccacagatc caaaaactgg acataatctg catggtggtg ctcctggatt
    25741 tgaattaaaa aaatggtctt atgtcatttt gaatggcgaa aatgaagctt ctgtcatttt
    25801 tactactaca agtcccgacg gagaacatgg ttttccaggt acaatggatg tggaaatccg
    25861 ctatactttg acaaaagata atatctggcg agtgacctct cgcggcacca gtgaccaaga
    25921 tactttattc aatccgacaa atcatgttta cttcaacttg acgggagatg cttctcagtc
    25981 aatcgatcaa catgagcttt ggctcaatag cgaagcttat gctcctttgc gaacagatag
    26041 tattcctata ggggtaaaag aaaatgctgc aggaagtgct ttcgattttc aaatacctaa
    26101 aaaattagcg tccgtttttg ctagtgattt ggatcaaaaa aatcttgttg acggaatcga
    26161 tcatccattt ttcttaaaag aaaccggctt gggtaaagaa gccgctcgct tgactagccc
    26221 cgataaaaga atccaagtag atattgctac agatgcttct agtgtggtta ttttcactgc
    26281 aaatttcggc acagaaacac cagaaatgcg caaccgaaaa ctggcacatc acggtggcat
    26341 cacatttgag actcaaacag ctccaggtgc agagcgcttc tcagcatttg gttcgattca
    26401 tttaaaagcc ggatcagtat ttgaaacagt aacagaattc aaaatcaaaa caagaaagga
    26461 ataactcatc atggaaatca aaagaagctt aaacacaaaa tttgccgaaa cattcggacc
    26521 taaaaaaact acacaatact tctcaccagg acgcatcaat ttgattggtg aacacacaga
    26581 ttacaatgga ggccatgtct tccccgcttc catcacttat ggaacgcaag gtgtagcagc
    26641 acctcgagac gataataaaa tattagttta ttccacaaac tttgacgata tcggtgtcat
    26701 tagttttact ttagatgagc tagaatatga taaaaaagca aactgggcaa attacgtaaa
    26761 aggaatggtc cttaaattaa aagaagctgg acactcaatc gatcatgggt ttgagttatt
    26821 agtggaagga acaatcccta atggtgcagg actttccagt agcgcttcat tagaattatt
    26881 agtaggtgtt gtattagaag atctgtttga tttaaaaaca gatcgcttgg aactagtcca
    26941 aacaggtaaa aaagtagaaa atgaattcat cggtgtcaat tcaggtatca tggatcagtt
    27001 tgccatcggc ttcggtgagc tagatcatgc aatcttatta gatacaaata cgctgaaata
    27061 cgaaatggtt ccagtgaaac tagatggtta tgcaatcgtc atcatgaata cgaataagcg
    27121 gcgggaattg gcagattcaa aatacaatga acgccgtagc gaatgcgaag aagccttggc
    27181 acgtttgcaa aaaactttgc ctatctcttc tttaggagac ttagatgaag aagaatttga
    27241 atcaactatt gaccaaatcg gcgatgatac attgatacgc agagctcgcc atgccgtttc
    27301 tgaaaatcag cggactttga aggcaaaagc tgaattagaa gctgggaact tagaagcatt
    27361 tggtcaactg ttaaatgatt ctcaccactc tcttcgctat gattatgaag taactgggat
    27421 cgaattagat acactagtag atgctgctca aaaacaagaa ggtgtcttag gtgcacgaat
    27481 gacaggagct ggatttggtg gctgtgcgat tgctcttgtc aaagaagaaa atatacctgc
    27541 cttcaaaaac aatgtctatg atgaatacat gaaagttgtc ggctatgctc cggaatttta
    27601 tgttgcacac attggaaatg ggacaacaaa gatcgatcca gaagctgttc atgtttgata
    27661 aaaaaatcaa cgatctcagc taaaaacaaa tagataaaaa taacctctca gcaatttcca
    27721 aatgtgagcc ttttgctcgt atttgaaaat tgctgttttt tattgacacg gaaagagaac
    27781 gtgtgttcta attgtgttga agaggtgatt gttatgtggg cgaatcaaaa tccggtattt
    27841 gtttatgaaa aagaaccttc aagagatatt ttatgtattg actgcaaatc tttttacgcc
    27901 tccgtagagt gcgttgaaaa ggatcttgat ccccttacaa ctaaattagt agtcatgtcc
    27961 tatccgtccg attcgacaga aacacggggt agcggattaa tcttggcttc cagtcctgca
    28021 gcaaaaaaag cttacggcat caccaatatc agccgtgcca gagatcttcc cttcccctat
    28081 ccaagtgatt tatatattgt tgccccaagg atggcctatt atatggaaaa gaacagagaa
    28141 atcaattcta tctataaaaa atatgcagac gaagcgaacc atcatgtcta ttccgtcgat
    28201 gaaagcttcc ttgatgtaac aaatagcatg gcactctttg gtaaaacaaa tgcgtacgac
    28261 atggctaaac tgatccataa agatgtgttt gagcagaccg gaatctacac gactatagga
    28321 attggcgata acccgctact tgctaaacta gccttagaca atggctcaaa agacggaaag
    28381 caaatgtttg ctgaatggcg ttatgaagat gtaccagaaa agttatggaa aatctcagat
    28441 atcactgact tttggggaat cggcaaacga acagcaaaac gtctgaaaca aatgggtata
    28501 cattctgttt atgatttagc tcacgcggat tattatcact tgaaaagcaa gctaggggtc
    28561 atcggtactc agctttatgc ccatgcttgg ggcatcgatc gaagtttttt gggacaagaa
    28621 tatacaccaa aatcaaaaag tatcggaaat agtcaaatcc tagatagaga ttatacaaga
    28681 cgagaagaaa tcgagatcgt tatcaaagaa atggccgacc aagtcggcac tcgcttgaga
    28741 cgatctgcag caaaaacaca agtagtcagc ttatgggtag gatactcttt aggctatctt
    28801 gatccaaatg gcaagaccgg gtttcaccag caaatgaaaa tcgaagcgac aaatgctagc
    28861 agagatctag cagatgcttt attgaccatt ttccaccagc attaccacta ccaggatatt
    28921 cggcgtgtag gcgtgaactg ttccgacctc gtatacgcga caggtttaca gcttaacctt
    28981 tttgaagatc ctgaaaagca gataaatgaa gcgaaagttg attttgttgt cgatacgatt
    29041 agaaaaaaat acggctttca atcaatcatc catgcgcata gtctcttgca aggaggacgc
    29101 gccatcgcac gtagttctct cgttggcggg catgcaggtg gtatgagtgg attagaaggt
    29161 ggaaaagatg agacggacag caaaaaaatt cattgactat aacgagtatc gagatcggcc
    29221 atttggatta aaatggggga ctgcttttgc gatggaagaa ctagtcaacg gtatcaaaaa
    29281 aatgaagaag aagcacgcaa acagcctctt cctaaacaac aaatgacatt gcaagaaatc
    29341 gatagtatac tgacagaagc ctttttatct cgtaaagcag taatcattca gctcaattta
    29401 aaagatgaat ttggaagact cttggatgag gtcgaagggc acttcattgg tgaagcctat
    29461 gaagattact ttatgattga ccatcagccg atattgtggg aagacgtacg gtatattgag
    29521 ttaaaggaaa caaaaaaatg gtttgtgaca tgagaggttg tgacagaagt gatcaacttc
    29581 aaaaaataag aaaggatttt tcgaaaattg cttcttaaat ttttgtggaa ttttggctta
    29641 tttttcgaag aagcaacttc tggaacaacg tatattagat atcagaggtt gtgaaaaagt
    29701 cgcttagttt tgagtaacaa gccaaggcgc tgatttcaca acctctgtct atctatccat
    29761 ctattcggta agattcatgc atgacgtctt ctttatttag tatgtagagc cctttttctt
    29821 ctgaccattc aacataaatc agctgtttta catctgcata gccttctttc ttcaagttct
    29881 ccataagcca tgcttcagtc aattgattgg actgaagcac tttatgctga atttgccctt
    29941 catctacaag caggatcgat aaagctttgt cttcatctga ttttttcaat acactcattt
    30001 gaccggcatt ctctaggata acatgtttgg cattttcaag aaaaaagcat tcttgctgtc
    30061 ttaagattgc tcgcaactgc tccatctcga tatggttatt tgtcaactct gtcatgttga
    30121 tgacgccgtc tttgattaga acagatggct cgcctttgac ccaacgattg accttttcat
    30181 ttttttggac gatacgttcg ataccataaa tcattactgc ccaaagcgca atggcaaata
    30241 ggacatgccc tacatggaca ttgtcatcat aggttgattc ttctaagatc ccacctaaaa
    30301 caagcgtata gactaaatca aacggtgtga tttctgacat ggatttcttc ccaagcaaac
    30361 gtaccactaa taataaaccg atcaacccga acaccagttt cattgtaatt gaaaaaatca
    30421 ttttttctcc ccctaactat ctgttaatta aattatatat gctttctcac gaacacgcat
    30481 ggaatataca aggaagaaat gtatcctttt actaaaaaaa ggactgtgac actgtagtga
    30541 cccccaaaag ttagactttt ttggagtgga gaaatccact cctttttttt atgctctaaa
    30601 atgatatttt tacacaaccg ttacggttgt tttacgaaat tggacg
//
LOCUS       DASNNU010000027        30508 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.027, whole genome shotgun sequence.
ACCESSION   DASNNU010000027 DASNNU010000000
VERSION     DASNNU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.027"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..205)
                     /locus_tag="U8116_001136"
     CDS             complement(<1..205)
                     /locus_tag="U8116_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434145.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRII"
     gene            complement(193..1545)
                     /locus_tag="U8116_001137"
                     /pseudo
     CDS             complement(193..1545)
                     /locus_tag="U8116_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(1542..2474)
                     /locus_tag="U8116_001138"
     CDS             complement(1542..2474)
                     /locus_tag="U8116_001138"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEN8434146.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2471..3247)
                     /locus_tag="U8116_001139"
     CDS             complement(2471..3247)
                     /locus_tag="U8116_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEN8434147.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3301..6486)
                     /gene="carB"
                     /locus_tag="U8116_001140"
     CDS             complement(3301..6486)
                     /gene="carB"
                     /locus_tag="U8116_001140"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEN8434148.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6491..7567)
                     /gene="carA"
                     /locus_tag="U8116_001141"
     CDS             complement(6491..7567)
                     /gene="carA"
                     /locus_tag="U8116_001141"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEN8434149.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7572..8852)
                     /locus_tag="U8116_001142"
     CDS             complement(7572..8852)
                     /locus_tag="U8116_001142"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEN8434150.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8871..9797)
                     /locus_tag="U8116_001143"
     CDS             complement(8871..9797)
                     /locus_tag="U8116_001143"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEN8434151.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9810..11093)
                     /locus_tag="U8116_001144"
     CDS             complement(9810..11093)
                     /locus_tag="U8116_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEN8434152.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11107..11646)
                     /gene="pyrR"
                     /locus_tag="U8116_001145"
     CDS             complement(11107..11646)
                     /gene="pyrR"
                     /locus_tag="U8116_001145"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEN8434153.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11881..12792)
                     /locus_tag="U8116_001146"
     CDS             complement(11881..12792)
                     /locus_tag="U8116_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN8434154.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12789..13253)
                     /locus_tag="U8116_001147"
     CDS             complement(12789..13253)
                     /locus_tag="U8116_001147"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEN8434155.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13258..13956)
                     /locus_tag="U8116_001148"
     CDS             complement(13258..13956)
                     /locus_tag="U8116_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN8434156.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13943..14329)
                     /locus_tag="U8116_001149"
     CDS             complement(13943..14329)
                     /locus_tag="U8116_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434157.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14476..16146)
                     /locus_tag="U8116_001150"
     CDS             complement(14476..16146)
                     /locus_tag="U8116_001150"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEN8434158.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16329..16562
                     /locus_tag="U8116_001151"
     CDS             16329..16562
                     /locus_tag="U8116_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEN8434159.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16661..17092)
                     /locus_tag="U8116_001152"
     CDS             complement(16661..17092)
                     /locus_tag="U8116_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEN8434160.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17171..17584
                     /locus_tag="U8116_001153"
     CDS             17171..17584
                     /locus_tag="U8116_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEN8434161.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17622..18770)
                     /locus_tag="U8116_001154"
     CDS             complement(17622..18770)
                     /locus_tag="U8116_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN8434162.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18892..19488)
                     /locus_tag="U8116_001155"
     CDS             complement(18892..19488)
                     /locus_tag="U8116_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN8434163.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19688..22330)
                     /gene="alaS"
                     /locus_tag="U8116_001156"
     CDS             complement(19688..22330)
                     /gene="alaS"
                     /locus_tag="U8116_001156"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEN8434164.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22718..23149
                     /locus_tag="U8116_001157"
     CDS             22718..23149
                     /locus_tag="U8116_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8434165.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23194..24543)
                     /locus_tag="U8116_001158"
     CDS             complement(23194..24543)
                     /locus_tag="U8116_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN8434166.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24613..25776)
                     /locus_tag="U8116_001159"
     CDS             complement(24613..25776)
                     /locus_tag="U8116_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN8434167.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25905..26228
                     /locus_tag="U8116_001160"
     CDS             25905..26228
                     /locus_tag="U8116_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434168.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26411..27760)
                     /locus_tag="U8116_001161"
     CDS             complement(26411..27760)
                     /locus_tag="U8116_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN8434169.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27829..28782)
                     /locus_tag="U8116_001162"
     CDS             complement(27829..28782)
                     /locus_tag="U8116_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEN8434170.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28884..30074)
                     /locus_tag="U8116_001163"
     CDS             complement(28884..30074)
                     /locus_tag="U8116_001163"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEN8434171.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         8574 a         6691 c         5352 g         9891 t
ORIGIN      
        1 agattattct cttctttctg ctaaccagtc ttagaaaaat gaattttaaa aaaatcaaaa
       61 actgatcacg atagaagttt cttttcatac ctgatgaaaa gatgcttctc gcaatcagtt
      121 tttggatttt tgctattcca attgatgatt ctggagacac aaatcggatt tctctatctt
      181 catcaagcgc gcttaaatct gccaattttc aggatctttt ttccattctt ttagcagagt
      241 taattcttgc tcatcgatat aacgatcttc taaagctgct tcaatcaata cagaataatt
      301 ggtcaacgtc ataagaggga tttcggcttt ttcgaaattt gcttttcctt ttggcaactc
      361 atacgtaaag atagccgcaa caccaaggat atccgcacct tctctttttg ctgcttcagc
      421 tgcttccaag acacttccgc ctgtagaaat caaatcctcg atcacaacca tcttctgacc
      481 ttcaaatata cgtccttcga tttggttacc tttcccatga tctttcgcct tacttcgaat
      541 atagaccatt ggcaaatcca gtatttccgc tacccatgcg gcatgaggga ttcctgctgt
      601 tgcagttcca gcaatcactt gtacttcagg gtaagcttct ttgattttgc ttgctaatcc
      661 ttttgcgatc tcttttcgga cttttggata gctcatcgtg attcggttat cacagtaaat
      721 tgggcttttg attccacttg cccatgtaaa aggattactt ggatttaaaa atactgcttc
      781 aatttctaat aagtcttttg cgatactttg ttcaacattc attttaggtt agctccattc
      841 tgtttgaatc gtttgatatg ctttataagg attagttgac tgagtgattg gtcgacctat
      901 aacgataaaa gttgagccga tcttttttgc ctgagaaggt gtgactactc ttgtctgatc
      961 tcctgcgact gttccttctg gtcttatacc cggagttaag caaacaaatt cgtctcctgc
     1021 catttctttg atcgcttcta cttcccaagc agaagaaacc acaccatcta atcctgcttt
     1081 tttggcacag ctagcataat ggatcacact tttttctaaa gaaacttcga ttagttgatc
     1141 ggcatgcatt tcttcttcac ttgtagaagt aagctgagtg atagctagaa gtagcggacg
     1201 ttttccacct tctggagtcc cctcttctaa tcctcgcatt gccgcctcca tcatacgaat
     1261 accgccagcg gcatgaacac aggtgagatc aacaccaagc tttgccaatc cccgcattgc
     1321 tttttcgacc gtattcggaa tatcgtgaag tttcagatct aaaaatacat tatgaccagc
     1381 ctcttttaga taacgtacga tttcaggtcc ttcttggtag aacaattcca ttccgatttt
     1441 cacaaacaat ttttcttctt tcgggaaaaa tcgaagaaag tcttctacct cttgcctagt
     1501 tgaaaaatct aatgcaataa ttggtcgttg gtctagttga ttcatcgttc acgttcctct
     1561 ctcacttctt tgattaatct ttccagcgat tcgatgccta gttcgctcat tcgcacaggg
     1621 agctctttaa ttagtttagg acagatatac ggatcggtga aattcgcggt ccctatagct
     1681 acagcacttg ctccagccat aaacatctca agaacatcat ccactgtctg cacgccgccc
     1741 atcccaatga ttggaaggct ggatatactt gctacttggt gaatcaaacg aatagcgaca
     1801 ggcttgattg ctggtccgga gagtccacct gtttgattgg ctaaaatcgg ttttcttgtc
     1861 ttaaggtcaa tccgcatacc aagaagagta ttgatcatcg taaacccatc tgcccctcca
     1921 gcttcgattg cctgagcgat tggaacgatg tctgttacat ttggggacaa tttgacatag
     1981 acaggaacag aagaaacttt ctttactgct tgtgttaatt gaaaagcaac atcagaatct
     2041 gttccaaaag caattccgcc atgtttgaca tttgggcaag aaatgttcag ctcgattgct
     2101 ttcacatttg gtgcttcgcc aattttgcta caaacttcta catagtcttc ttcacaagca
     2161 ccagcaacat ttgcgatgat gggtaactca ttgtactcct ctaattctgg aagaagttta
     2221 tgcataacaa cgtctagacc aggattttgc aaaccaattg cattcaacat gccagatggt
     2281 gtttccgcca ctcttggcgt gggattgcca aagcgggcat ttggagtcgt tgccttgacc
     2341 atgatcgatc caagctgatt caaatcatag tattttccgt attctttgcc aaaaccgaaa
     2401 cagccgcttg ccggcatgat aggatttttt aattctaggc caggaagttt gaccgctaaa
     2461 ggattctcat tcatataatt acctctccta ttttaaaaat cggtccttcg tcacaaactt
     2521 tcacactttt tgttcctgtt tcatcttccg gcgtatgaca aacgcaagca tagcaagccc
     2581 ctatcccaca tgccatgcgg gattctaatg ataactggac attttgatgc gaagaaaaca
     2641 gctgttccac tgtcttcaat aacccgtttg aaccacatgc aaaaacagct gtaggttccg
     2701 tcttcgttcc taacaataaa ttaccgacat ttccgtgcat accgtatgtt ccatcgtcag
     2761 tagcgattct tgtttctcct agacgattaa attctttttc ataataaacg acttcatgtg
     2821 tggcaaaacc taaaaaatgc gttggcttaa ttcctttttc gactaactgt tttgataact
     2881 catatagagg agggacaccg attcctccac cgattataaa tacttcgtct cctgtatgaa
     2941 gattggacaa atcaaaacca ttgcctaaag gacccatgac atctaaatgg tctccggcag
     3001 aaagagtaga gaagactcta gtcccctcac cttccacccg ataaatgata cggcaagtat
     3061 ttgtttcgtg gtcgatatga ttcaagctaa tcggtcggcg taatagcaag tctgcacgcg
     3121 gcacgagaat atgcaagaac tgtcctggtt gctgcatttc ttttactaga tcacctttca
     3181 atgtcatttc aaaaatcttt ggtgccagct gtttttgctg aacgatttcc atcatttctt
     3241 gtttcatctg tcccctcctg caacggtttt tcgtcttttt gtcttaacat gtgctcgccg
     3301 ttaaataggg ttagttgaaa aagcacggga ttccatcact cgaacgatcg catctgctgt
     3361 gtctaaagaa gtaaacaatg gaataccatg ttcaactgct tctcttcgga tgatgaaacc
     3421 gtctttagaa gcatttcccc gatctttatc aatcgtattg acaaccactt gtgttttacc
     3481 atctcttatt aagtcgacta catttttttc tgctgcttcc tcagaaattt tcgctactgt
     3541 ttcaacgacc agaccatttt tttcgaaata agcagctgta tgtttcgttc ctatcagact
     3601 atagccaatt tctgtaaaac gtttagctaa ttctagtgct tcttcttttg tttcgtccgc
     3661 aatcgtaaat aatacggctc catattcagg caagtgtaat ccagaagctt cgaatgcctt
     3721 gtacaaagct ttgtctaatt gtctgtcaga tcccattact tctccagtcg atttcatttc
     3781 aggccctaag taagtatcaa ctttctgcaa tttcgtaaaa gaaaagacag gtgctttaac
     3841 atgaacttct tttgtttctg gataaagtcc gttttcataa ccaagtgtct ttagtttctc
     3901 tcccaaaata gctttcgtcg caatttgtgc cattggaatt cctgtcacct tacttaagaa
     3961 tggaacagtc cgacttgctc gaggattgac ttcgatcaca taaacctcat tttcatggat
     4021 gacaaactgg atattcatca tcccgacaca attcaaacct aatgctaagc ggatcgtgta
     4081 atcttcgatc gttttcttga tcgattcaga taatgtttga ggtggataca cagccattga
     4141 atcccctgaa tggacaccag cacgctcaat atgctccatg atccctggga tgagtactgt
     4201 ttcaccgtca caaatcgcat ccacttcgca ttcaccacca agcaagtaac ggtccactag
     4261 tactggatgt tctggagatg ctttcacagc atgttccatg taatcttcta aatctcgctg
     4321 gttttcaacg atttccatcg cacgtccgcc taatacatag cttggacgaa caagaactgg
     4381 ataaccaatt ctcgaagcga taaccacggc ttcttcttta cttgtagcag tatcccctaa
     4441 aggttgagga atatccaact ctttcagcgc ttgctcgaat aaatcacgat tttccgcacg
     4501 atccaagtct tcgatcgttg tccctaagat ttttacacca gcagcagtca aaggttcggc
     4561 taagttgatt gctgtctgac caccaaattg aacgattact cctatcggct gttctagctc
     4621 gatcacattc atcacatctt cgaaggtcaa tggctcaaaa tataatttat ccgaaataga
     4681 aaagtcagtc gaaactgttt caggattgct gttcatgatg attgcttcgt atcctgcctg
     4741 ctggattgct ttgacagaat gcacagtcgc atagtcaaat tcaacccctt gtccgatacg
     4801 gataggacca gatcccagta ctagaattga gggcttttct gatcgtgaac tttcattttc
     4861 atattcatag gaactataaa aataaggtgt ttttgattcg aattccgctg cacaagtgtc
     4921 aaccatctta tatacaggaa tcaaatcatt ttcttttctc atctggcgaa cggcttcttc
     4981 gtttatttcc cacagctccg ccactttttt gtctgtaaaa ccgttttgtt tcgctgtttt
     5041 caatatcttg tcatccaacg gctgctgttt caactgctgt tcgatttcta cgatatgcaa
     5101 cagtttatct aaaaagaata aatcgatttt tgtcaaatcc gctaattctt ctatggtgta
     5161 ccctcttcta atagcttcag ctacataaaa caggcgatca tcttgtgctc gaaccacttt
     5221 ttggattagg tcatcgtccg tgatgtctgc caactcagga agttcgatat ggtgtgtacc
     5281 gatttctaaa gaacgcacag ctttcaataa agactcttcg atattccgac cgatagccat
     5341 cacttcacca gttgctttca tctgcgtgcc taaaagccgt tctccgtttt caaacttatc
     5401 aaacggccag cgaggaattt tagctacaac ataatctaat gcaggttcaa attctgcaaa
     5461 agtcgttcct gtaacaggat ttttcatttc gtctaatgtt aatccaagcg ctattttagc
     5521 agctagctta gcgatcggat agccagtcgc cttacttgct aacgcagatg aacgagaaac
     5581 acgcggattg acttcaatca cataataatt aaaactatgt ggatcaagag cgagttggac
     5641 attacagcct ccctcgatct taagtgctcg aataatcgaa agagaagcat cacgaagcat
     5701 ttgatattca tggtcagaca atgtttgact aggggcaaat acgatagaat cacctgtatg
     5761 gatacccacc gggtcaaaat tttccatatt gcagacaacg attgcattat cagccgaatc
     5821 tctcatcact tcatattcaa tttctttaaa acctgcaata cttcgttcaa tcaaacattg
     5881 ggtagcgggg gaaagactta atccattttc tgatacgata cgtaattctt cttcattttc
     5941 acacatacct ccaccagttc cgcctaaagt aaaggctgga cgaacgatga caggatagcc
     6001 gattttattt gcaaaatcta cagcttcttc tactgtattg acgatttcag actcggggat
     6061 tggctgattc aattctttca tcaactgttt gaatagatca cgatcttctg cttgatcgat
     6121 cgcagaaagc tttgttccta gaagttctat gttcaattct tctaaaatac ctgatgctgc
     6181 cagctccatt gccatattga ggcctgtctg tccgcctaaa gttggaagga gagcatctgg
     6241 acgctctttt cgaagaatcc ttgatacaaa ttcaagtgtg atcggttcga tatatacttg
     6301 atctgccact tctttatccg tcatgatcgt cgcaggatta gaatttacta aaaccacttc
     6361 atatccttct tcttttaagg caaggcaggc ttgtgttcct gcgtagtcaa attctgcggc
     6421 ttgaccaatg atgatcggac cagatccaat caccataatt ttcttgatat ctgtacgttt
     6481 cggcatctgt ttactgctcc tttcctgcgt ccatcatttc cataaattca tcaaataaat
     6541 gtagtgcatc atgcggacca ggggcagcat ctggatgaaa ctgcacactg aaagcgggat
     6601 aatctctgtg tttgactcct tctactgttc cgtcatttac ttctacatga gttacgatga
     6661 gtttttctgg atcaatcgtg gcttcatcga ctgcatagcc atgattttga gaagtaaaat
     6721 caattcttcc ggtcgctact tctcgtaccg ggtgatttaa tcctcgatgt ccgaatttca
     6781 ttttataggt atctgcaccg ttggctaaag caaataactg gtgaccgaga caaataccaa
     6841 aaatcggtac ttttccttgg atttttttga tcatttcgat tgcttctggc acatcttttg
     6901 gatcaccagg tccattcgtc agcatgactc cgtctggtga tagcgctaaa atcgtctcgg
     6961 catctgtgtt atagggaagt actgtgaggt tacagtttct tttactcaat tctcgtaaaa
     7021 tactatgttt caagccgaag tcaatgacaa tgactttccg acctgttcct ggacttggat
     7081 aaggtttgtt agttgagact tgctgcactt gattggttgc tagaacggtt gctttcagct
     7141 gatcaaatgc atgttcgaag gaatctccag catcaactat actagctttc atcgttccga
     7201 cttcacggat tttgcgagta agggcgcgcg tatcgatacc tgatattcca ggaattcctt
     7261 tttgtttcaa aaatgaatct agcgtcattt ggtttcgcca gtttgaagct acacgtgcat
     7321 gttcttttac tactactcct ttgcatgttg gagaaatcga ttcataatca tctctattta
     7381 ccccataatt acccaccata ggataggtaa atgtaatgat ttgaccgtta aaactttgat
     7441 cagtgatagt ttcttgataa ccagtcatac ttgttgtaaa aacgatttct ccgaagacac
     7501 tagcatctgc accaaaagcc tttccttcga aaactgttcc atcttctaat ataagcaaac
     7561 gttccatact tttattctcc cttctgccac acaagttttc cgttgacaaa agtcatttgt
     7621 gtttcgcctt ttactttcca gccgataaaa ggtgtattct ccccttttga taaaaagtct
     7681 tctttatcga tcgtgcaagt ttgtgtaata tcaaaaactg ctacatcagc gggggcacca
     7741 accgttagcg ttcctgcatt caaaccaaaa atctcagccg gtttaactgc caaccaattg
     7801 atcacttgtt ccaacgtgaa tcgaccagtt tctacaaaat gagtatagat cagttgaaac
     7861 gctgtttcgc ttcctacgat accaaatggt gatttcatga aactttggct tttttcttct
     7921 aatccatggg gggcatggtc tgtagcgatg caatcgatcg taccgtcaag caagccttcg
     7981 atcaaagctt ctctgtcctc ccgtcctctt agcggcgggt tcattttcca aaagccgaaa
     8041 tcttcgggaa tatcttcgtc gatcaaaata agatgatggg gtgaaacttc tgctgttacg
     8101 tggattcctg ctttttttgc gtctctgatt acacggacac tttctttagt agaaacatgg
     8161 caaacatggt aatgaacacc agttgcttca gctagcaaca gatctctagc aatttgagaa
     8221 gactcggtta cgcttaagat cccgggaaga ccaagttctt ctgccttctt gccagcgtgc
     8281 ataacgccgc caaataataa agattcatct tctgtatggg ctactagggc tttattgttt
     8341 tttgcagctt ctttcatagc cagatacatc gtcccagctg tttggacacc tacaccgtca
     8401 tttgtgaaag caaaagcacc ttcttcaatc agtgcttctt gatcaactaa tttctcactt
     8461 cgcagatttt ctgtgattgg cgcatactgc aataccttca caactgcatc ttttctaatg
     8521 atgtcatata cttgtctcag cttttctgcc gtatcaggta caggatttaa attcggcatc
     8581 gcgcatacag tagtgaaacc gccacgagca gctgagcgag tcccagcttc gatcgtctcc
     8641 ttgtatgtaa atcctggctc tctcaaatga acatggacgt ccactaaacc aggtgtgatc
     8701 aattgtccct ttgcatcaaa cacttcatca aattcggctt ctgagaatcc tgtaccgatt
     8761 gctttgattc gcccatcttc tatccaaatt tctgctggtg tcgtcatatt ttttcgtgta
     8821 ttgatctgac cgtttttaat gagtgttttc attttttcat cctcctataa ttacgctttt
     8881 ccatttagca ctgcttctaa gattgccatt ctgacgaata ctccattgct catttgctga
     8941 acgatccgcg actgtaatcc ttccactaat gaatcagcta attctacatc acggttaact
     9001 ggtgctgggt gcataatgat tgcatgtttt tgcaacttct tcgcacgctc aactgttaac
     9061 ccatgttcct gatggtagcc ttcttttgag aaactttctt ttccatcatg gcgttcgtgt
     9121 tgcacccgaa gcatcatcat gacatcaact tgatctagta gttcatccaa tggcatataa
     9181 tgtccgtaca cttcgaattc atcgtcgtac cattctctag gacctgagaa gaatacttgt
     9241 gcgcctagac gttttaacat ctgcatattg gatttagcta cgcgagagtg agtgatatca
     9301 cctacgatgg caactttcag accttcaaaa tgaccaaatt cttcataaat ggtcatcaaa
     9361 tcaagcaagc attgggttgg atgctgtccg cttccgtctc ctccattgat aatcgaacac
     9421 tggattgttt tactttgtat cagttcgtca taatagttct catcaccatg acggataacc
     9481 gctacgtcta cgcctaatgc tgacatcgtc agtactgtat cgtatagtgt ttcccctttt
     9541 tggacagagc ttgtgcttgc ttcaaattca atcacttcta gccctagttt tttctccgct
     9601 acatcgaagc ttttatgtgt gcgagtacta ttttcgaaaa agaggtttgt agcaaagtac
     9661 tggcgctttt gcggagtcca attagcacct tgcttgaatt cctgccctct gcggatcaag
     9721 cccataactt cttgatcagt aagtgcctca acggtcaata aatgctttaa gctgatacgt
     9781 tctgatttga tgatcataat gtttcctcct tagttttctt cgcttcgtga agtttctggt
     9841 aagataagat ttaaaatgat gccaagtaca gttgctaatg ccattgccga caatgtaaat
     9901 gtgcctactt caaagacgag tccgccaatt cctactacta agataactga agcaatcaac
     9961 agatttttct ttttatcaaa atcgattttg ttctcgatta aaatcttcat tccacttgcg
    10021 gcaattactc caaataagat aaaactgatt cctgagatga ctggtccagg gatacttaaa
    10081 atcaaagcgc ttaattttcc tacgaatcct aaaatgactg caaatactgc agctccaccg
    10141 atcacaaaca cgctatggac acgtgtgata gccagaacac caatgttttc accgtaactt
    10201 gttacaggag gacctccgac aaaggctgcc acgatttgtg ctaatccatc acctgtcaac
    10261 gtcttatgaa gacccggttc ttcaaagaaa ttacgtttag ttagtttatt caataccata
    10321 agatggccga tatgctctgt catcgtcaca aaagcgatgg gtgccattgt tgtgattgct
    10381 ccaagatata gctttggttc atattgtaca aagggaatct caaagttagg aacagcaaac
    10441 caggcagcgt ctacgattgg cttagtatcc acgattccaa acagcaaggc tacgatgtat
    10501 ccgctaataa tacctagtaa aatcggaatc aatccgagaa aacctcttag ccacatgttg
    10561 aagaaaattg ttaatcctaa tgtgatcagc gctacagcaa tatacttgaa atcatacgtg
    10621 ccctgattat acatagcatt tgttgctgca tttgccgcaa gtcccaaccc gataaccatg
    10681 atgactggac cgacgacgat tggaggcaag attttatcca gccatgctga accgatcttt
    10741 ttgatgatca gcgcaacaat caaatagact aatccagttg tgactgctcc ttgtgcaatc
    10801 gctggataac catctgtttt catcaaaagc tgcatcgctg caatgaaagc gaagctgctt
    10861 cctaagtacg caggtatctt tcctttagta gtaaataagt acaccaatgt acctagtcct
    10921 gaactgacta gcgcaatacc tggatcgata cctactaaga tggggactaa cacggttgct
    10981 ccgaacatcg tgaacaagtg ttgtaaactt aatcctaccc aatgtcctgg ggttgggcga
    11041 tcgtggatat ctaacaccac gtcatcatta tgaaatcctt ttttctccga catgtttctc
    11101 tctcctctaa tcttcctctt tcaaaatcat gatgcgatct tgtccatcaa gctcttgcat
    11161 ctctactaaa atctcttccg cccgagatgt tggaatgttc ttcccaacat aatctgcacg
    11221 gattggcaat tctcgatgcc cacgatcgac caggactgct aatgaaattt ttcttggtct
    11281 gccaaaatcc attactgcgt ccattgctgc tcgaatcgtt cgtcctgtat acaaaacatc
    11341 atcaatcaaa ataacttctt ttccttctaa ggaaactggg atgtcagaag aatgaagttc
    11401 cggttcttct ggattttctt ttttgtcatc tcggtataaa gtgatatcca gttctcctac
    11461 aggaatatcg atatcttcta gctgcttcag gcgttcggca attcttgatg caatgtagat
    11521 tcctcgtgtt ttgataccta ccaatacgat atcttggata ctatggtttc tctcgataat
    11581 ctcataagta attcgagtca atgctcgctt catggttacc tgatctacta cttcttttgc
    11641 ttgcattttc attcccccta cttttttgca taaaaaaact cctgccctaa gaggacagga
    11701 ggaatgttta gactgagcgt atactgatac actgatagct atactatgtc aatgagcaat
    11761 ctacttatgg cgcaccatac gtatccttgc tcccaccctt cttagcctca cgggactaaa
    11821 cttaaaggaa ttctattaag ttttatgctt tcgttgttaa ttactatacc gttatctaaa
    11881 ttaaaatgca atgttttttc ttaatttttc taaagttttt tcaaaaactt ctggaagagg
    11941 tgcttcaaag atcatctttt cgtttgtagt cgggtgcgta aaacctaaca tttgcgcatg
    12001 gagaaactga ccattgcctt tcaaagtttt cttcggtccg tatactggat ctccagctaa
    12061 cggataaccg atatacttca tatgcacacg gatctggtgt gttcttccgg tttctagttt
    12121 taattcaacc agagtaaatc cttcgaaacg ttccaataca gtaaaatgtg tgacagccga
    12181 cttcccatct tctcttatcg cctgcatttt ccggttttcc ttcgaacggc cgattggcgc
    12241 attgatggtt cctttttcgt gaggaatctc tccatggact aaagcaacat atttgcgcaa
    12301 agaggtctta tcttttaatt gctgagctaa tgattcgtgt gcctggtcat ttttggcaac
    12361 cattagcagt ccggaagtat ctttgtcgat tcggtgaaca atacctggac gaataacgcc
    12421 gttgatcgta gaaagatcct tgatatggta catcagcgca ttgaccattg tgccatgcgt
    12481 atgacctgct gaaggatgaa cgaccattcc ttgaggtttg tttatcaccg ctacatcttc
    12541 atcttcgtaa acgatcgtca acgggatatt ttctgggacc acatctaatg tttctggttc
    12601 ggggatcaag acagtaatct cgtctccttg ttttactttg taatttgctt tgactggctg
    12661 tccattgaca gaaaccgctc catttttcag ccacagctga atttgcgaac gactgtaatc
    12721 ggtgaataaa ttagtcaata ctttatcgat cctaccagat tcatggtgaa tcgtcgtttt
    12781 aagttctgtc atttttttct ttgatccttt tcatctaaaa taatataaat taacatacaa
    12841 atcactccac atacaagtgt cacatctgct acgttaaaaa tcggaaagct cataaaatct
    12901 gtctggaaca tatctactac atagccaaga cgaatacggt cgataaaatt gccaatcgca
    12961 ccagctagaa tcaaactcaa tccaatcatg aaccagcgat tacctttttc acgattttta
    13021 atcatcaatg tgagaatcac aacgactgca atgagtgtca ctatagtaaa gaaccacatc
    13081 ttcccttcta gcatactcca tgcggctcca gtgtttcgaa tatgattcag tgacaaaata
    13141 cctggtataa gatttttcgt ctctccaagc gctagattat cagtgatcca ccatttgacc
    13201 aactgatcca ctcctactag caaagcgctg aatatccaat aaataattaa caaaaaatca
    13261 atccttttct atttgtttat aaatatcacg aggtcgtacg atcccgagca gattaccatc
    13321 gggattccca tctttagtga taagtacggc ttccaaagtc ccttgctcac gaaacatcgc
    13381 ttcaacctcg taaatatgtg ttttagctgg aacaaaatga taatttgtgt actttccgtt
    13441 ttggatgatc aaatccaacg ctcgtttatt tgttaaatca accggacctt tttggctttc
    13501 ttttgctagc caaaacccga tattacgtaa agtaatcaat gctacaaatt ttcctttatt
    13561 gtataacgga aattgtgaat accttttttc tgctactatc tttaacagtt caagtaacgg
    13621 aagatcttgt tcaaaacctg ttacacgttt tgcaaatcga ggcagcacgg tttcaggctg
    13681 aatcagttct tgttcgatca gctggattcg cttgactgtc caatcatttg gctctgcaat
    13741 gacaaaatcg actgcgatcc gatcatggac gatcgcattc cttaactgag caagctgtaa
    13801 aagatcatct tcgtattttc tgatcattaa ttgttttctt tgagctaggc gtctgacaag
    13861 ttcggtaaat cccatatttc gtgcatttcc catctcgtct ctcatccatt tttcgattcg
    13921 attgaaactg attaaaaaaa gatcagagtt tgccataatt ttcctccttg atcaaatatg
    13981 cccggttttt tatatagaaa aagaacgcaa tcaacacaat aactgcaaaa aaactgaaaa
    14041 ataaattttg taggaaaacg gcccccaacc cagttgctag aaataaaaac ggatagattt
    14101 gaagaatcaa gtaattcatt gtgatcacat aagcaggctt tgcttctttc actgtataaa
    14161 cggggctttt tagccaataa aaggtgacct gcatcgtaaa gggcacttcc agatcaagcg
    14221 ttatcttttg ttcatgactg agaacactgc gtttttcatt gttgattctt agctgaatcg
    14281 agctacccat accgtaaaaa ccggtttttc ttataaatgt cgttttcatc ataccctcct
    14341 attatgcttt agtatagctc aaaatacaat aaagaaaaag aatgatccta gtgtaaacaa
    14401 aattaattcc ctatcgtcta aaaaaagaag ctgtagtcaa aatacagctc cttcgatggt
    14461 agattataat caagcttaaa acaatcccaa cacttctccg tcatttgtca cgtccatatt
    14521 caatgcagca ggttttttag gcaaaccagg catcgtcata acatctccag tcaaggctac
    14581 taaaaatcct gcccctaatt ttgggatgat ttcgcggatc gtgatggtga aatcttccgg
    14641 tgcacctagc agtgaaggat catcagaaaa tgaatactgt gtttttgcca tacaaatcgg
    14701 cagtttgtcc catccattct tcgtaaactc ttctatttgt ttttgcgctt ttttgctgaa
    14761 aacaacacca tttcctccgt aaattttctt gacgattgtt tctgtctttt cttgtatcgt
    14821 ttcttctagg cggtataatg gcttgtaatt agcttctttt ctatcaatca atcgtactac
    14881 agcttttgct aaatcaacgc cgccttctgc tccatctgcc catacactcg ctctcttggc
    14941 aagaatcccc tgattttcac acaagtgctc caacagtgtg agttcattgt ctgtatctgt
    15001 tacgaattca ttgattgcga cgatcacagg tagctgatat tgttccatat tacgtatatg
    15061 gcgctttaaa tttgcaaaac ctactttcaa agcatcaaga ttctcatttt taagttcatc
    15121 ttttttcatt ccgccatgca ttttcaatgc acggacagtg gcgacaatca ccacagcatc
    15181 cggcgctttt ttcaagttag gtactttgat atctaaaaat ttctctccgc caagatctgc
    15241 accaaatcct gcctctgtga caacgtaatc tgctaatctc aatgctgtct tggtagctaa
    15301 aatactattg cagccatgag cgatattggc aaacggtccc ccgtggacaa aagctggtgt
    15361 tccgtaaatt gtttgtacaa gattcggctt gattgcatct ttcagcagta atgccagagc
    15421 gccttctgct ttcaagtctc cagcagtcac aggctgacgg tcaaatgtat agccgacaac
    15481 gatccttgca agacgagcct ttaaatcttc aaggtctgcc gcaagacaca agatagccat
    15541 gatttcgctg gcaactgtga tatcaaaccc atcttctctt ggaacacctt ggattggccc
    15601 acccaagcca acaattactt tacgcagttc acgatcattg agatcgacta cccgtttcca
    15661 aatcactcgg cgggcatcga tgttcaattc atttccttga tgaatatgat tatctaaaag
    15721 agcagataat gcattattag ctgttgtgat agcatgcatg tcaccagtaa aatgcaagtt
    15781 gatatcttcc ataggcaata cctgtgcata tcctccgcct gcagcaccac ctttgattcc
    15841 catcacagga ccaagtgagg gttctcgcaa tgcaataaca gattttttat tgattttatt
    15901 gagcgcgtct cctaacccta cagtgatcgt tgactttcct tcgccagctg gtgttggatt
    15961 gatggaagta actaagatca aatgcccctc agcgttatcc tttgatcgat tgattccaga
    16021 aaaattgatt ttcgccttat atttcccata cagttccaaa tcgtcttcct taagatctaa
    16081 tttactagcc acttcaacaa tcggttctag ctctgtttct tgtgaaattt gcagatcatt
    16141 cttcatttga ttaccccttt tcacgaactt tattcaacaa aatatcttct ttgttaagat
    16201 atgccaatta tacctttttt tattcagaaa aaaaacataa aattacaggt ttttaattta
    16261 tttgacgtac ttataaatac tctttataat gaaggctatg atcagacttt agaaaggtcg
    16321 tgagtgcttt gaccacagga attgtaaaat ggtttgacaa caaaaaagga tacggcttta
    16381 tcagttatga tgacacagaa gaaatttttg tacattttac tgcgattgaa gaagagggat
    16441 tcaaaacttt ggaagaaaat caagtggtcg aatttgagat catcgaaggg aatcgaggaa
    16501 cacaagctgc ccatgtcaag aaaggcaacg ttcagactac cgatactagt atgcatgaat
    16561 agataaacaa atagagtgag acgctaaacg ggatgaaccg tacgtctcac tcttttcttt
    16621 tcctgtaaaa attcgttttt ttaggttctg atttttaaaa ttagcttttt ctttttttcg
    16681 tataaggata aggaattgat tctgatttgg gtccgttttt ttcaaaatga tcaaaaagga
    16741 ctacttgatc tttttttcgc atcatgaaaa cagatggggc tttctcatct tgcaacatca
    16801 tttgcacccc agtattagtt ggcgtcaaag atcgaatctg tccgacagat atttctcggt
    16861 ctttccaaaa agcagcataa cgtataatca cagagtttga ttttaaaata aaagaacgcc
    16921 gcaaacctag aaatagaaaa acaagaaaaa tacctaaaac aaaattgctt ttccaataag
    16981 gacctgtgtt ttctaaagaa agaatcagac taaaaaacag aatgatcaag gtcaatgacc
    17041 aatagatgat cgtatgtgcc agttcaggct gccatcgaat attttttttc attctctaac
    17101 ctctcaccga ttcagtaatg atataatctt accataacat tagtttgaac aaaaaggaga
    17161 atctcttttt atgttaaaag tatacattga tgcctctaca aaaggcaacc ctggtcctag
    17221 tggaggtgga atcttgattg tctatcaagg taaacaagaa cagctatcta tcccgctttc
    17281 tatagggaca aaccatcaag cagagttcga agtcttttta aaaactttag aatttctaaa
    17341 gaaaaacgac ttacagaatg aaacgatttt ctgtttttca gacagcaaaa cactagtttc
    17401 tacggtcgac aaaaacgcaa cgaagaacga gctcttcctt ccctaccttc aaagaatcca
    17461 agaacttcta tccgaatttt ctttattgat cctgcaatgg ttgcccgaaa acaaaaataa
    17521 aggagcagat aatcttgcac gtcaggctct ccataaacaa ttaaaagaaa aaaagactct
    17581 ttaaattaaa aactttaagg agtcttttta tagtttctca tttaatataa aagataattg
    17641 tgatcaaacg taatttccaa ttggtaatct ccgatttttt cttctatttg actacgtaag
    17701 attctttcta gttctgtatc aacaaactgg aatttctcag ggaccactac atcaaattgc
    17761 agcgtttttc cttgatgaat tactctaaaa tcatgaatat taagccctgt acctgtttca
    17821 agaagaattt gctttaactg ccgcaagata ttatagtatt tttcgttatc aacaggcatt
    17881 ggatccaagt gacagacaag ttccacatcc aaagactttt taaagtcttt ttctatcaga
    17941 tcaataatct tatgagccac attcaaattc attgattcat ctacttcgat gtggactgat
    18001 gcaaagcgtt tattgggtcc atagttatgc accaataaat catgatagcc aagtatatca
    18061 tcgcaagcat ttaaatgctc ttccatttct tcaatctctg ctggcgttgg gcgacttcct
    18121 aatagttcgt atacaaaatt tcgaagcatc gtcaatccac tataaaggat atataatgct
    18181 aaagcaaaac ctatatatcc atcaatacgc catccagtta cccattcgat tgctgctgaa
    18241 agcaatacag ccagtgtagt aaagacatca ttataactat ctgttgctgt agctctcaat
    18301 gtttcagaat caatggattg cgcaataacc tgatacattt tgccttgtgc aaatttcaaa
    18361 aagatggaaa tgattaggac aaaaaagacg atgggtgtta aagagacatt ttctggatgg
    18421 atgatttttt cagcagaaga ctctaaaaat tgaaatccta cataagtaat gatgatcgaa
    18481 acaaataacc cactgatata ttcgaagcgt tcgtgtccat aaggatgttc ctgatctgct
    18541 ggctttgcag caattttaaa tccgatcaaa gtcaatatgg atgaagcagt atctgataga
    18601 ctgttcactg catcagccat aatggagaca cttcctgaaa aaagcccgat cattagctta
    18661 gagacaaaca agatcagatt cgtaaccaat cctaagatac ccgctagtac tccttttttt
    18721 gtccgctgtt gcggtatttg tttctttttt tcttcttgtt tcttagccaa gttttttacc
    18781 ccttctagtt tgcttgttgc tcaggtaaaa agatggagag gacgattgtt ttcctctcct
    18841 ctcttccttt ttctatctat cgtgcgtcct attactatta aaagatggtc attattctgg
    18901 tttcaacggt gttgctgcaa ataacgcttt tgctagtaat ggtgttatga ttcccgcacc
    18961 tattgcttct ggtatcccat tgattgctgc aacccctgct aaggttttaa ataaaagagc
    19021 agagtctaca ccataggcat ctgccgttgc acctgtataa aataatccca tgaatcctaa
    19081 cactaagact gtattgacca atgaacccag tacccctgca aaggctgagc tgatcgttat
    19141 tgatttattt ttttgataaa tccagtggaa aaccaagcct gctactaagc caacaagtac
    19201 tctgggaact accgagataa tcggattcgt gaacactaat gtatcaaatg gcgttgttgg
    19261 catggcatag gcacgaacaa ttgtagcgat tccccaaaac aagccaatca ccatgccatc
    19321 ttttgggcct aatactactg cagcaataat tactgtgata tggataattg ttaaactaat
    19381 gaatcctaat ggaataaacc ccagccaagg aaccatcgct tgtacgatga tgattgctaa
    19441 caagatcgca cgtaagacta gtcgaaaggt cttatttcta ctattcattt tttcacccct
    19501 ttttatttat actaatcgta aaaatttctg tcactttcgt caactaaaga ccatccgaat
    19561 ccgaccattt gattagatgc gccatccaat tgaaaaaagt atgagggcaa gacttgtttg
    19621 tcttcgcccc cataatcatg tttcgttcaa tgtttgaact tttgtttttc tgtcaaacgt
    19681 aaacgcccta ttgttgcgca acccattctt tcgcagcttt aagtgcatct ggaataccag
    19741 caggattttt cccgccagct tgtgccatgt ctggacgtcc accaccgcca ccgcctactt
    19801 tcggtgcgat tgctttaatc aagtcgccag cttttagtcc agcttcattt gattctttcg
    19861 tcattgccgc tagcagactg acttttcctt cttgttcgtt tgctaaaaca agaatatctg
    19921 aaatattttt ttgtttccat tggtcagcta attgacgcag ctgattcata tctttcactt
    19981 ttacttgtgc tgcaatgatt gtttttccat tgatcgtttc tggatctttg aatacatcac
    20041 cagcttgttg attcgctaac ttgctagcta attgttcatt ttccttttgt agctctctta
    20101 cttgctgttg caattgttcc gcttttgaaa caacttcttt caattgaggt gatttaacaa
    20161 ttgttgctac ttgttttagt tgtttttctt cttcttcaag tagttcgtac gcttctttac
    20221 ttgttacagc ctcgattcgt cgcacgcctg caccaattcc tgattcagaa acgattttga
    20281 agattccgat gttttctgta tttgctacat gtgtgcctcc gcacagttcg attgaccagc
    20341 cgccaatatt tactactcga acgtcatgtc cgtatttttc accaaacaaa gccattgcac
    20401 ccatgttttt ggctgtatcg atatctgttt caattgtttc aactgggata gcttcccaga
    20461 ttttttcgtt gacgattctt tccatttcag ctaattcatc tgaagtgatt tgaccaaagt
    20521 gggtaaagtc gaatcgtaaa tgtccaggag cgactaaaga accagcttga tttgcatgct
    20581 cgccaagtac ttctttcaat gccttgtgca gtagatgggt tgctgtatgg tttttgatga
    20641 tccgattgcg catcgattga tcgatcaata gatgataggt ttgtccttca ctcattggtg
    20701 cttttacttc tacggtgtgc ataaattgac cattaggcgc tttttgaaca tctgtaacaa
    20761 ctgctacgat ttcgcctact tcattcatga tccatccttg atccgctact tgcccaccca
    20821 tttctgcata gaaaggtgtt tgatcaaaga tcatttgagc ttcaccttct gcgacttcat
    20881 tcaaaagaga ctcaccttta acgatgacca ataattgact atctgattct tgttctgtgt
    20941 atccaatgaa tttactttct actttgatat ctgtcaataa agcagattgt acccccattg
    21001 atttttcagt actgcgggca gagcgagcac gttcgcgttg tgcatccatt tctttttcaa
    21061 aaccagcatg atcaactttc agtccttcat cttctgccac ctcttccgtt aattctaccg
    21121 ggaagccata tgtgtcgtat agtttgaaga tgtcttttcc tgctagagtc gtcccgtttt
    21181 cgttttttac ttcttggatc acttgattta agatttccaa accttcattg attgtttcat
    21241 tgaaacgttc ttcttctgta cggataactt tttcaataaa ttctttttgt tgaagtactt
    21301 caggataata acttaccatg atttccccaa caacagggac taaacgatat aagaatgctt
    21361 gttcaatacc taattttttc ccatgcatga cagcacgacg aagcaaacgg cgtaagacat
    21421 accctctccc ttcattcgat ggcaatgcac cgtcgccaat agcaaatgaa agggcgcgaa
    21481 tatgatctgc gattactttg taagaaatat ctgttactgg gttttcacca tattttactt
    21541 gtttgcttag cttttctacg gcgtggataa taggcataaa caagtctgtc tcaaagttcg
    21601 ttgacgcatc ttgaacgatt gagaccatgc gttccaatcc catccccgta tcaatgtttt
    21661 tatgaggaag tggttcaaat gtgttatcag gcttatggtt gaattctgaa aatactaagt
    21721 tccagatttc taaataacgt tcattttctc ctcctggata gttttcagga tcgtcttctg
    21781 ctaaatcgtt ataagcttct ccacggtcat agaaaatttc cgtatccgga ccacatgggc
    21841 ctgcaccgat atcccagaag ttgtcttcta catcaataat gtgatctagt ggcaggccaa
    21901 cttcttcatg ccagatgcgt tttgcttctg tatcttttgg ataaactgtt acataaagtt
    21961 tttctggatc gaatgccatc cattttggat cagtcaaaaa ttcccatgcc cagtggatgg
    22021 cttcattttt gaaatagtca ccaatcgaaa aattacccag catctcaaac atcgtatggt
    22081 gccgtgctgt tttaccgaca ttttcgatat catttgttcg aatagatttt tgggcatttg
    22141 tgattcttgg attttctggc acaactgaac catcaaaata ttttttcaat gttgcaacac
    22201 cagagttgat ccacaataag gttggatcgt tcacggggat caaagacgca ctaggttcaa
    22261 tcgtatgtcc tttagattgg aagaaatcaa gaaacatttg tcgaacttgt gcactactta
    22321 attgtttcat gtcattacct gctttctata aattaaatac aaaaaaacac catcacagcc
    22381 aaggacgaaa actcgcggta ccaccttgat tgcaatgatg tgtaatcatt acctcttaaa
    22441 ctcgataacg ctgagctgcg tcggtatttc taccaaaata gaaaaaggga gcaatttgat
    22501 tagtgactat tcttttttca gccaagaaag aattttctgt gagtcttaaa aaacaaatca
    22561 tatccttttc gtacttaatt agtatacgca ttctattgat cactgtcaat atctcaattt
    22621 ttattttcat ttccttttgg tcatactcca cgatttttgg tacgataaga ataaaggaat
    22681 caatacatct ctgctattca tgacaaggag gaatatgatg aaaaaaatag ttggagcgga
    22741 tgaatggata cgcgcagcta gctgcttttt gagatatcaa gtttttgtat tagaacaagg
    22801 gattcctgca gaactagaat tcgacggaaa agatatcccg caacaaacct attatctatt
    22861 tcttgatcaa aaaagaccag tagctacttt gcgttatgaa aagaaaacac ctacttgctt
    22921 caagccggaa cgattttgcg tagcaaaaga ataccgagga aaagggttag gcggtcagtt
    22981 gcttgaagct gctgaaaaac gggcggtttc agaaggttgt cagactagtt atgtagtagc
    23041 tgaaatgacg gctgtagatt tttatgaaaa acaagggtat catacttgca cagaaccttt
    23101 tattgaagat gaagtacttt gtatcggaat gaaaaaaagt ctagcttaaa aacaagagat
    23161 tgtgacacgc cttttgtcac aacctcttgt tttttattta tgtgaatttt tcttattttt
    23221 acgagcagta cggctttgct gtctgcgttc gattttgcgt tcttttttat ttttttctgc
    23281 aattgcccaa tcgatttttt tcttataacc aggcttgatc tttttctttt tcttcttcac
    23341 aagaccaatc agcgtgggat ctaatacatc ttttgatttt tctcgtttgg cacgtctatt
    23401 acgatcgtat gttttgacga tttccccgtt tttgatttct tttggttcaa atgagacgcc
    23461 caatttttct atcgcatcaa tggcttgctc atcatttggc gaatataacg taatggccgt
    23521 tccttttaag ccattacgtc cagtacgtcc gacacgatgg ataaagaagt ctagatcttc
    23581 tggaatttct gcattaataa catgagagac accttcgata tcgatccctc tagcagctaa
    23641 atcagtcgct acaacatatt gatactccag attctgtact tgtcgcatca ctcgttttct
    23701 ctctcttggg gtgatgtctc catgaatctt tgctactttc aagccttggt ttttcaagta
    23761 atcggcaatt tcatccacat gctgttttgt attggcaaat acgattgcta aataaggatg
    23821 accaattgtc agcagttgat aaatcatttg attctcatct ttaccttttg ttgaaatcag
    23881 ccaattttca atgtcttcag aaataactgc ttttggtttg atttcttcaa tgattggatt
    23941 ttccatgtat tttcgtaaga atggacgaag tttttctgga atagtggcag aaaaaactaa
    24001 aaactgcagc ttttctggta atcgacttgc aatttggtct acttcattta aaaaacccat
    24061 atccaatgtc atatccgctt catcgacaac aaatgcaaat gcggtgtgaa tcttcagcgc
    24121 ttgttcattc atcatgtcta atattctacc aggcgtgcct actacaacct gtggctgttg
    24181 gtgatgcaga cgatttaatt gacgctgttt gtctgtaccg ccgacaaaat tcgagacacg
    24241 gatctccggt gtagaaaatt tagcgatttg tgtagctgct tcatagatct gtgcagctag
    24301 ttccctactt ggagctgtaa taacaacttg tacttcctct ttttctgcat tgattttttc
    24361 aaacaaaggc aacaaaaacg tatgtgtttt tcctgtacca gtttgagatt gcccaatgat
    24421 attttttcct tttgcaatta aaggaatcaa acgttcctgg acctctgtgg gctgtttgaa
    24481 gccctttgcg gttaacgctt cattgataaa cggtttgaag cggaatatat caaaattatt
    24541 cattcgtgca cctcttctct ttaaattatc acgactttgg cattataaca cagacaaagc
    24601 gtttttgctc agctaatttc gagaatcatc atcctttgtt tggccaatcg tgctaacagt
    24661 atatgtgtga ctagcttttt cataaaacag aatccgtttt tttctcaaaa atgatagcaa
    24721 gatatttaaa ataaaaatac cttgtagtaa caagacaccg agagaaatga atactgcact
    24781 catctgcaac accgtattcg ttgtcgaatt catgatttgc tgaagcaact gccccaacca
    24841 gcttaaacca taaaaaagaa agtacagata cccgtagcgg atcatcaatg cttttaaaga
    24901 aattcgttcg cggtcttctt ccaccaaacg gatacgcaca atttttttgc caagtgtcca
    24961 tccattggtt ataaacggta caatcataaa gtatacccct acttctaata aaagccacca
    25021 gatatgagaa gtaactgccc ctgaatcgaa cgcaatttta aagaaaatcg ccgctaccaa
    25081 attaataaga gataaaaatg tccagtcgat caaataagcg accaagcgtc gagtcaatga
    25141 gacagtcttt cctttttcat aagaaagttc atccattcgt cttcttgtag gaagcataaa
    25201 tgtaaataac ggcgtaacga caaaaccaat gattccaccc aatgtatttg tcactaaatc
    25261 attcacatca aataagcggt atggtcttgg gtagataaaa taaagtccac ttagctgcgt
    25321 caattcaaaa aatagagaaa ggcagaaact agcaatcatc gttttcataa aagaaagccg
    25381 gaaataatat cttaaataaa tgccaaacgg tatgagtaat aaaacattaa aagctggttc
    25441 caaaaataca ctctgcttca ttgccggcag gtaggtacta ggttcagaca agaccaatac
    25501 cgtgttgtct ctgaaatgtt gccatgaagc aaataaatgc agctccatct ttggccctgt
    25561 catttgggca accgtctcct ttgccggaag tggtaagatc accaaaaaat acgcacacaa
    25621 taaataaaaa acgaaagaat acaaaataac tgcccttgtt actagaaatg ttccatactt
    25681 tcgatattca taaataaaaa aaacagatga gatcgctaag gctaaaaatg gaaaaacaat
    25741 cagcgcaaaa cgaatcggtt cgatataact tgccatatga ctcctccttg attctttgta
    25801 tctctcttat cttagcagtc ctttgctgtt tcaagcaagt aacgaggcta ttatttctca
    25861 tttctttcaa agtaaagtaa gataaacaca aagggggtgt cgagatgctg aatatgtact
    25921 ttgtttttgg cgtacctatt tttttactgt tcctttatgc tacgatcgct tatgtaagaa
    25981 aaagaactac aattcattat ttaggattta ttctcctgat tatttcgggt ttcatgctag
    26041 tcttcaatct tcagacttgg cagcaagcac ttcttgaaat ggataaaatg actccacatg
    26101 ctttgtctaa agtgcttggc taccctgtct atctgatttg gcttcccatt ttcatttctg
    26161 gttgcttggt tttattaaat atttatcgcg gcgtacgccg aatagttcag ttacgcaaat
    26221 caaagtaaaa gacagaaatc ggttgagccc gatttctgtc ttttactttg ctatttcatc
    26281 gtagacaaaa cgacatttac gtgatcaaaa ttaacgagta cttttgacat ttcttccacg
    26341 tactcctcat ttaaataaaa tattttgttt ggcagagaag tgcagttcta ataaaattcg
    26401 aatgttttag ctatctacgt tttaaataga gataaatcgg gataccagca agtgtcaata
    26461 ctatccccat cgaagctaaa aaggtctgag tgataagcgt gcttgatacg ataaaagtcc
    26521 ctccaatgat tgctactagt ggaataaacg gatacattgg aactttgtat ggtcgaaaca
    26581 aatcaggttc ttttttacga agaagaatga caccaacaaa gaccatcgta taaaaaatcc
    26641 aaatgacaaa gatcagcata tccgttagtg tatcaaaacc accgatcatc atcatgccaa
    26701 tagcgatgac tagctctaag atacctgcgg ctacaggaac tttagtttta tcatgaagtt
    26761 tagcaaagag cttgctaaaa ggcaggttgt tttcttttgc cataacataa gggagacgca
    26821 ttccagtaag ggtatagcca ttgatggttc cataaacaga aatcaagata ccaactgtga
    26881 ccagtcttcc accgaagccg ccaaaaatca tttttgcaac gtcacttgcc gcattgctgt
    26941 ttcctgcgac tccgtcgata gatgcggttc ttaagaacac cgcatttact aataaataaa
    27001 cgatcatgat tccgatgatt cctaaagaaa ttgcttttgg caaatctttc gccggctttt
    27061 tcaattctcc cgaaatattt cctacgtgta tccaaccgtc gtatgcgaac atcgttgcta
    27121 gcaagcccgc tcccaaggct gagaaaaatg aaagattttc tcctgcttgg atcggaaaca
    27181 gttggaagtc tactccttct tgtcggaaca aaccgaaaat gacgatcaca aacaaaggaa
    27241 taagcttgca gacaagcgta atcgactgaa aagcccctcc agctttagaa cctaaaaaat
    27301 tgatcaacag gatcgataca gcagctgtaa cagctacggg aacaatcatt gactgggaaa
    27361 gaccaaatag attgacgaac tgtgtaccga aaatgatcga caaggctgct acatttgctg
    27421 gaaaataaat cacgacttgc gcccagccca ataaaaaagc tgctgtattt ccgtaaatcc
    27481 gttcgatata tttaatcatc cctcctgttt ctggaatagc agctgccagt tcagctcctg
    27541 ttagtcctgc acagacagaa atcataccac cgagaaacca agaaaacata tgcaaactgg
    27601 cggatcctgt aacttctgct acgcttgcgg ctttaaaaaa tacccctgca ccgatcactg
    27661 tgcccataac agtcgataaa gcagtaaaaa atcccatcgt acgttttaat tcattttcta
    27721 tttttacttc ttttactggc gtggcttgat attcttccat cccttgtccc ctttcatacc
    27781 atagccaatc ccaaaagtat aaaaaacttt ttggatgtgg ttcttgtttt ataccgtctc
    27841 taccaatttt tctttcagtt tttccaacat atcctttgtc atcacatcta agtcgtattt
    27901 cggcgaaaag ccccattcct cttgcgcaca agaaacatct agtgaatttg gccaagattc
    27961 tgctattgac tgacgtacag gatccacatc ataatccatt tcaaaatctg gtatgatttt
    28021 ttgaatagat gctttgatct cttccgggtc aaaagacatc gccgatatat tgaaggcatt
    28081 tcgatgaacc aatcgatcag gatctgcatt catgagtttt ataatggcat cgatcgcatc
    28141 tggcatatac atcatatcca ttgatgtacc ttgatcaatg aaactagtgt actttccttc
    28201 tttgattgct gaataataaa tatcgactgc gtagtctgtc gtaccgcctc ctggcaaggt
    28261 tgcataagaa atcaatccag ggaaacggac accacgagta tctacgccat atttcacata
    28321 ataataatca catagtagtt ctccagctac cttcgttacc ccatacatgg ttgtaggacg
    28381 ctgaagagta tcttgtgggg tatgatcttt tggagtattt ggaccaaacg cgccgattga
    28441 acttggcgta aaaaatttca gctggaattc tctagcgact tccaatgcat tgaccagccc
    28501 agtcacgttc aaattccagg ctaattgtgg ttttgcttct gcaacagctg acaaaagagc
    28561 agccaaatga atcaatgtgt ccacttgata ttgttctgct atgctgcgca ttttttcgta
    28621 atccattaca tctaggatct caaagatatc gtttttctca atgacttcag gcatgcgtat
    28681 atctgtcgca atgacatttt cactgcctag ttcttgacgt agccgtgtga tcaactctga
    28741 accaatttgt cctaaacatc ctgtcaccaa tacttttttc atgatgatcc tccctgatct
    28801 tttttactac tatcttcaga tgattaacaa tatatgcgtg aacatgttct ctaattttaa
    28861 atagacgaaa aaactaaatt tagttaaata atttccattt cttttcctat tttttgataa
    28921 actgctactg cttcatcaag catttccctc gtatgttccg cagtcggcat atttctgatc
    28981 ctgcctgtcc caagaggcac agttggataa acgattggtt tagcataaat cccatgttcg
    29041 atcagcttct tagagaaagt ctgagtcact ttttcgtctc ccaaaatgac tggagtaata
    29101 ggtgtttcag acataccgat gttatagcct acttttttca attcttcttt aaaataatta
    29161 gcattttccc aaactttttc gacaagttcc ggatgttctt gcatcaaagt aatcgaagcc
    29221 aatgcagctg cagctgcacc tggagttagc gaagtagaaa ataggaatgg ccgacttcta
    29281 gctttcaacc agtcgattag tgttttcgat ccagctacat aacccccaac aacacctata
    29341 gctttcgata acgtgcccat ttgcatatcg attttgtcgc ttaatccaaa atgcttgacg
    29401 gttcctgcac cttttccagt tacgcctgag ccgtgcgcat catcgacgta ggtgatcagt
    29461 ccatattttt caacaatcgg aataatttcc ggcaatcgtg caatgtctcc atccatggag
    29521 aaaacaccat ctgtgatata catgattttt ttatatttct tactttctat cgcttccttt
    29581 gcttttttct caaggtcttt catatcttga tgtttaattc ggatgatttt tgctccggat
    29641 aagcggcaac cgtcaataat agatgcatgg tttaattcat ccgataagat agcatcttct
    29701 tttgtcatta ctgcagagat agcgcccata ttgcagttga atccagattg aaaggcgatt
    29761 gctgcttctg tccctttgaa ttctgcgatt ttttcttcta attgctgatg gattttcagc
    29821 gaaccattga tcgttcggac tgctcctgct cctacaccat aagtttcggt tgcttcaata
    29881 catgcttttt tcaattcttc gcgattggca aatccaagat aattgttgga tgctagattg
    29941 atcatctttt ttccgtctac gatgatacaa gcaccatttt cactttctag tatgtcgatc
    30001 gtattgtata accccttttc tttcagttga tccaattctt tctctaaata cattgttagt
    30061 tctttggaag acattgtctc cacctccata ttcatctgtc ttgcagaaca taccaaacat
    30121 atatcgtgtc attcatctac aaagcaagta tgctatattt ttgataacgc ttacactgat
    30181 tatgagaatt atttaaccac attttttatt tacagtatct ttttactttc aaatgactta
    30241 tatagtatga acaattacct tttagataat gatctatttt actctacctg ggcagataaa
    30301 tacaacacaa ccattgatag aagccctttc gttaaaatca attgtattct gaatatttaa
    30361 tttatttatt gcatagacca ataaaaatac ataaaatttc catcttcgtt tatcagcaat
    30421 ttttaatttt actctctgaa tatttcttaa aagttttact tgttgaattc gaaaaaagga
    30481 ctgtgacaaa agtcttgtca cagtccta
//
LOCUS       DASNNU010000028        29879 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.028, whole genome shotgun sequence.
ACCESSION   DASNNU010000028 DASNNU010000000
VERSION     DASNNU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.028"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..304
                     /locus_tag="U8116_001164"
     CDS             5..304
                     /locus_tag="U8116_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN8434172.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            377..1738
                     /locus_tag="U8116_001165"
     CDS             377..1738
                     /locus_tag="U8116_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEN8434173.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1760..2020
                     /locus_tag="U8116_001166"
     CDS             1760..2020
                     /locus_tag="U8116_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434174.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2034..2786
                     /locus_tag="U8116_001167"
     CDS             2034..2786
                     /locus_tag="U8116_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEN8434175.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2880..3485)
                     /locus_tag="U8116_001168"
     CDS             complement(2880..3485)
                     /locus_tag="U8116_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN8434176.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3538..4512)
                     /locus_tag="U8116_001169"
     CDS             complement(3538..4512)
                     /locus_tag="U8116_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEN8434177.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4890..5609)
                     /locus_tag="U8116_001170"
     CDS             complement(4890..5609)
                     /locus_tag="U8116_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN8434178.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5736..7007)
                     /gene="celB"
                     /locus_tag="U8116_001171"
     CDS             complement(5736..7007)
                     /gene="celB"
                     /locus_tag="U8116_001171"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN8434179.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7160..8557
                     /locus_tag="U8116_001172"
     CDS             7160..8557
                     /locus_tag="U8116_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN8434180.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8638..9078
                     /locus_tag="U8116_001173"
     CDS             8638..9078
                     /locus_tag="U8116_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN8434181.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9226..9552
                     /locus_tag="U8116_001174"
     CDS             9226..9552
                     /locus_tag="U8116_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN8434182.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9553..10302
                     /locus_tag="U8116_001175"
     CDS             9553..10302
                     /locus_tag="U8116_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434183.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10382..10837)
                     /locus_tag="U8116_001176"
     CDS             complement(10382..10837)
                     /locus_tag="U8116_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN8434184.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11264..11539)
                     /locus_tag="U8116_001177"
     CDS             complement(11264..11539)
                     /locus_tag="U8116_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434185.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11833..12648
                     /gene="thiD"
                     /locus_tag="U8116_001178"
     CDS             11833..12648
                     /gene="thiD"
                     /locus_tag="U8116_001178"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEN8434186.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12977..14428)
                     /gene="cls"
                     /locus_tag="U8116_001179"
     CDS             complement(12977..14428)
                     /gene="cls"
                     /locus_tag="U8116_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN8434187.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14769..17435
                     /locus_tag="U8116_001180"
     CDS             14769..17435
                     /locus_tag="U8116_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN8434188.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17799..18551
                     /locus_tag="U8116_001181"
     CDS             17799..18551
                     /locus_tag="U8116_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN8434189.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18573..19727)
                     /locus_tag="U8116_001182"
     CDS             complement(18573..19727)
                     /locus_tag="U8116_001182"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEN8434190.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19912..22353
                     /locus_tag="U8116_001183"
     CDS             19912..22353
                     /locus_tag="U8116_001183"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEN8434191.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22510..23784)
                     /locus_tag="U8116_001184"
     CDS             complement(22510..23784)
                     /locus_tag="U8116_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN8434192.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRDTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(23963..24610)
                     /locus_tag="U8116_001185"
     CDS             complement(23963..24610)
                     /locus_tag="U8116_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN8434193.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(24618..25304)
                     /locus_tag="U8116_001186"
     CDS             complement(24618..25304)
                     /locus_tag="U8116_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8434194.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(25310..26383)
                     /locus_tag="U8116_001187"
     CDS             complement(25310..26383)
                     /locus_tag="U8116_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8434195.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            26605..27279
                     /locus_tag="U8116_001188"
     CDS             26605..27279
                     /locus_tag="U8116_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN8434196.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            27284..28624
                     /locus_tag="U8116_001189"
     CDS             27284..28624
                     /locus_tag="U8116_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN8434197.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(28773..29759)
                     /locus_tag="U8116_001190"
     CDS             complement(28773..29759)
                     /locus_tag="U8116_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEN8434198.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         9428 a         5349 c         6034 g         9068 t
ORIGIN      
        1 aaaaatgaaa aatcggttaa ttgtcgcatg tggaagtgga gtagcaacga gtcagacaat
       61 cgctagtaag atccaaagta tgttagaaga tgacggcatc gcatttcctg tagaagcagt
      121 tgattacaaa tcgattcaaa atgagcttct aactgctgga atctatgtgt atgtcgcaca
      181 acctgatgag gaagtacttg aacaagcaaa agatttgggt attgaagtat ttccaggaat
      241 tccattttta actggaatgg gtgtagaacc tatttatgac agtatcaaag agttagtaca
      301 gtaaaggagg attgttggat aaaaaagttg gttcatagtt ttatttacta tatttagaaa
      361 gaaagaaggt ttatttatgg aagttttcca aaccattatc aattatgttt tgaacttagg
      421 atcagccatt tttgttcccc tgattatttt gatacttgga ttgttggcag ggatgaaatt
      481 aaaaaaagca ttcatgtctg ctttgacttt agggattgct tttagcggaa tgtctatggt
      541 cattggctat atgtctaacg cagtaagtcc agcatcagaa gcattggcta aaaatacggg
      601 aatcagtttg cctgcacttg atcttgggtg gacaggtgca gcaagcatca catggtcgtg
      661 gagttatgct tttatatttt ttgcagtaac gattggggtc aattttgtac tgttactttt
      721 taattggacg aagacgttaa atgttgatat gtggaatgtt tgggggaaag ctttaactgc
      781 ttatttagtt tattatgttt ccggtagttt ggctgcaggc tttcttactg caatggtcca
      841 agttatattg gaattaaagc ttggtgatat gtttcaaaaa catatacaag atctcacagg
      901 gattccatta gtaacagtga ctcattttat gacatctgcc gctgtcttgt tgctgccttt
      961 caatatgatc atggataaaa ttcctgcttt aaataaacga gcggatacga atgcattgaa
     1021 gaagaaaatt gggatcttct ctgaaaactc tgtaatggga ttcattattg gattggggct
     1081 tggctttgct gctgcgtatg gcgtttcagg ttctcttaat ctagcgatcc aaacagctac
     1141 agcattaagt ttgtttccta tgatctctaa aatgttcatg caatctttgt ctcctctagc
     1201 agacgctatg tcagaattta tgaaaaagcg gtttaaggat cgtgaagtat atatcggatt
     1261 ggactggccg atattagctg gtcgttcaga aatttgggtt actgctattt tacttgttcc
     1321 tgtgtttatt ggttatgcca ttattttgcc aggaaaccaa gtattgccat tagcagggat
     1381 tatcaactat tcgattgcgg tcggcggatt acttttaaca ggaggaaact tggctcgtat
     1441 gctgatcttg ggaatcatca ctatgcctat ttatttgtac ggggccactt accttgcgcc
     1501 aatactcagt gatttagcag aaaaaagcgg agcagtcgag ggaatcaaga aaggacagtt
     1561 gatcacatgg tcatccatag aagggccgga gttcagggtt ttatttgcgg aggcatttaa
     1621 taggaatatt ctcgctattg taggatgcgt gatttttcta ggactgtttg ttctacttta
     1681 taaatatatg ataaaagcga aagtaccgag tgaacgttat gctgaactac aaaaatagca
     1741 ttgaaatggt gggaaagtaa tgaaaagaga agagacagat aaaataaaat ggacagtcgc
     1801 tctttgtggt acacttctgc tatttctata tggactcttt actcagaata tcatcatcaa
     1861 tctattggtc atttttttcg cactagtgat ctataaatat ggaaatcacg ttttattcag
     1921 agagtacgat gaaaaaagaa aacggaaaat agaagaatca atgaaaataa aagaagctac
     1981 caaagaaata ttgagagaaa aaagttttat aaaaagataa tggaggggtc attatgtcag
     2041 atggacgtat gttatttgag agagaacgac aggacatggc acgtatcgta agagtgattt
     2101 tcgaaaggaa aaacacaaat gtcgcaggag ggaatttttc ctttaaaacc actgatgatc
     2161 aagggaaaga ctacatcatc atgacgccga ccatgatgtc agaagcctat ctaggagaga
     2221 tttctcctgc acaggttcta gtagtagaac caagaacgcg tagaatcgtt gacggagtcg
     2281 gtcatttaac tcgtgaaatc aatatgcatg aagcgatgta tgagactaat ccagctatca
     2341 aagctgtcct acatgctcat gcgcctaatg caatgttctg ggcgactact gggttgccta
     2401 tgcctaattt gacagaagcc acagctaaag taaaagagat tcctgtcatg gactttgagc
     2461 ctaattgctc tgaagaactt gctgaattga caagcgacta tgtaaaaaaa cagcatctcg
     2521 aattaccgca tatgttgctg ctcaactctc atggcgtatt gatcaacaca ggtggagaat
     2581 caggaatcga agctatccat aaagcactgc aaatccttga tacagttgaa tggaatgcag
     2641 aaattgccta caagcagacg atttttcaag cattagggat tactgacggt ttttattcaa
     2701 aaggtgagaa aatcggggga ttggaagagt tgaaacataa aacggctatt tataataaat
     2761 ctgttattgc agctggtgga gactaaccta tctgacacaa aaaactacga ctttcacaaa
     2821 atctgatcca gatcgatatc tgcttcaggt tgtattatgt aagtcgtagt tcttttcttt
     2881 tatttagtta agaggtggtc tgtccattcc ctcgcttgct tgatttcgtc catcgtcaac
     2941 tgatgacctg ttacagttag ctgaatagtc acatttgctt ggcgtgtttc gaattgatcg
     3001 gccaattctt taaaggaagc aggggaaacg attggatcgt tatcaccata agaaatccag
     3061 acggttttat cctctaatgt ggaacggctt tgatgaacac ctagagacat cggatggaaa
     3121 aacaaccctt tatttagctc ggtctcacgc tccaataata aatgagcagc gatgtttgcg
     3181 ccatttgaat agccgacaag cagccagtca cttaacggta tcagttcttt ttcgctgatt
     3241 tctttgattt tttcatggag tttatctgtt tctttttcca aactgtccag gtcgaattga
     3301 ttcaatccat tacgcttgaa aaaacgattc atcccatctt cttggaccgt gccacgaaaa
     3361 gaaagcaatg tactgtttgg tgcaagaaag tgagcgatat ccagtaaaga atgttcatcg
     3421 ccacctgtgc catgaagtaa gatcaaatgc ttgccacttg ttcctttttc aaaaatgtag
     3481 gtcattttgt tccctctttt ctgtttctat agatgcaaat gaagtagcat gaagagatta
     3541 ttcacgtttt ttatttgcat cacttgtatc gaaaggacgg acataagctt caatggcttc
     3601 acgacgttct tctaagaatg gaggaagaga tagtttttct cctgctgatt ctgctggttc
     3661 atcttcaagg aatccaggac catcagttgc taattcaaac agtacgtgat gagcggctag
     3721 gaagtactca gattggaaat agaaacgatc gacgaaacca gaatttggca attcaatctt
     3781 attgattact tgaatccagt attccaatgc ttctttgtct gctacacgca atgctaagtg
     3841 atggatattt ccaaatcctt cgattgcttt tggcaagtct gtccgatgtt caataatgat
     3901 gcttgcacca tttccccctt caccgatttc aaataagtag aaatctcctg aatggtctgt
     3961 ttctttgaat cctaagattt tttcaagaat caagcggata tgctctaatt gactgacacg
     4021 gacaaagaca ggtccaagtc ctgtgatcgc aaactctgtc ggtacattgc tgtttttcca
     4081 aggaatgcca gctgctacac cttgattgtt ttcgtcagag atcaattgat aatactgatt
     4141 atcgaaatct tcaaattcga tatatttttt gccgaaacgt tcattgattg ctgaatgagt
     4201 aacgtcatat ttttcaaaac gttccaacca aaagtctaat gctgcgtcac ttggcacacg
     4261 gaaagaagca cgagagatcg tatctgttcc tttttttcct tttggctgat ttggaaaatc
     4321 aaagaaagtc atatctgttc ctggcgcacc agtatcgtct gtaaagtaaa gatggtaagt
     4381 ttcgatgtca tcttggttaa ctgttttttt gattaagcgc attcctaaaa tatctgtaaa
     4441 aaagcggtag atttttgtag cgctagatgt cattgctgtg acgtggtgaa ttcctctgat
     4501 ttgtgtatcc atgataaatt tcctcctgtt aacttacaaa aagtaagtag ttgatttaga
     4561 catagtatag agggtgcatc tctattttgc aattaatttg tcttctaaag tatcaggaag
     4621 gaatcaagaa tggaaaagag gagggacgga tcaataaaat aaaaagactt acgactagct
     4681 gtatcgattt caaaaaaact agctttaaaa agctaacttt agaatcatta cgggtggtca
     4741 taagtctttt tatgctgagt tattttttca tttttgagct aatgcttagg atagaataga
     4801 atgttgattt cacgatctct tcttgttact tgaaactgct taccactgtc acgatctctt
     4861 ggtaaacggc tttttcacaa cctctttggt taagttgaac gcacatccag cacagaatag
     4921 ttccgaacat caaatcggtt tcggctggaa gaatattcaa tcggctgtcc atctttgaga
     4981 tagacgactt gctgaacttc cagtacagga tcgttttctg cacagttcaa atattgctga
     5041 tcatattcat cacttttatc tgcagaaata gcacgataag cccctgcaaa atggacattc
     5101 aattcttgat gaagatgatc atagatagag gctaacaaat gttctttttt taaacccggc
     5161 acaagatgga ctggcataaa ggtatgctct aaaacatatg gctcgccatc taagatcctt
     5221 aaccggtgaa tattgtagac aggttgctcg gcatcgatca tcaatcgttc ttggatatat
     5281 tgattaggga attcaacatc aaaatgaatg atttgactct tcaaagaacg atgtctgcgt
     5341 ttcatttgga gactcaaacc ttcatactcg ttagcgggcg atgtgtcttt ttgcataaat
     5401 ggatgattca agattttcgt tcctgatcct cgctgcgaat agacaagtcc ttccattgct
     5461 aagatattga tggctttttt gattgtcatc cggctcgcac caaattcttt taccaagtcg
     5521 acttgattag gcaaaaaact atcgagagga tactcttttg aacggatacg ttctcttaaa
     5581 atatttgcga tttcttcgta ttttggcacg ttattcacct cttctagagt ataacgaaag
     5641 attatgcttc cgacaactcg aagtagcatc tggaacattg tttattcgaa aaaagaggtc
     5701 atgacaagtt tttgtcacaa ccccttaatg atctattaag caatatctaa atctggttgt
     5761 ttatctagta ctttcaggaa tgggtaccag ataaaggcaa cgatgacaaa gtcgatgact
     5821 tgcagcatac caccaagcca tgaatttgtt gctaagacac cgcttgcaat gattggcaca
     5881 gtccatggaa cagagacgcc agttggtgca gggacaagtc cagcagccat taccaaataa
     5941 ttgaacgtcg ttacgattag aggtgccaca acccaaggaa taaggattgt tgcattcaat
     6001 acgatcggta gaccgaagat cgctggttca tttacattga aaataccagg tgctaaagct
     6061 aatcgtccaa catcacgata ctgtttcttt ttaagaacaa aggccatcaa taaaacgaca
     6121 gctaaagtca ttccagagcc accaatacca acagtgaagg tttccatgaa tggttttgtg
     6181 atgatgtgtg gtaattcttt tccatgttga taagcttcta agttatcaag catcagtgta
     6241 ttccaaatcg ggtccattac agagttgacg atgatttgtc cgtgaagtcc gaagaaccat
     6301 aagaattgga caaagaacaa agcaatcaat gttgcaggta aactacttcc caaaccagtt
     6361 aatggttttt ggatcacttc ataaacgacg tcatgtaagt tagcattgaa aataccagtc
     6421 atcacagcat tgattactaa gaaaatagtt agtgtcacta cagcagggat caaagcggca
     6481 aatgattttg ttactgcagg cggaactcca tcaggcattt tgatctgcca gccgcgtttc
     6541 gtgattctgc agtagatttc tccagccaag aaagcagcga tcattccaat gaacattcct
     6601 tttgcgccaa gacgatcaag tgaaagcaca ccagtcacat cttcaccgcc agcggttttc
     6661 attgcaaaag gcgtaagaat caagaagctg gcaaaagaca cggcaccacc gaaaatacct
     6721 tcaacgtcat aagattttga taaataatag ccgataccaa aagtaacaaa aaccgacatg
     6781 atgctcattg ttgcattttg cccattaccg aataaattcg ataaagttcc ctttgttgcg
     6841 tcactgaaaa aaggcaagtt attgataacg acaacgatcg aaccgaacat cgttaaaggg
     6901 aaggaaagca tgaaagcatc ccgcagcact gtaaggtaac gattttgccc taataaatta
     6961 gctaagggca tgattttctc agaaagcttg tccataaaac cgttcatttt tattcacctc
     7021 aaatttattt gcaatcgatt acatacaaag tataacgcca aaaattttaa atgtctagtc
     7081 attttcataa aaagtattga tttatttttt agggttcgtt atgattacgg tatcaagaaa
     7141 aaagagagga agtaaaaaaa tgaaaaccta cacattccca gaaaattttt ggtggggttc
     7201 tgctgctagc ggaccgcaaa cagaaggaag agtaagtgat gatggtaaag gagaaaatat
     7261 ttgggatcat tggtatgaat tagagcctga aaaattcttc gatcaagtag gacctgaaaa
     7321 aacttcgcaa gtttacacaa aatacaaaga agatgttcag ttaatgaaag aaactggcca
     7381 taacacattt cgtacatcta tccaatggag tcatttgatt ccagaaggga caggtgaaat
     7441 caatcaaaaa gctgtttctt tttatcgaga ttattttact gaattgctgg ataatggtat
     7501 tgaacctttt gtgaaccttt accatttcga tatgccacta ccactgcaag aaaaaggcgg
     7561 atggttatca agagaaacgg tcgacgctta tgtttcttat gcagcagcgt gctttgaact
     7621 gtttggagat ctggtcaaaa aatggtttac tcataatgaa ccaatcgtac ctgtagaagg
     7681 aggctacttg tatcaattcc actatccaaa tcatatcaat atgaaagaag cggtccaagt
     7741 aggctatcat gagaatctag caagcgcaaa ggtaatcaaa gcctaccatg aaggcggaca
     7801 tgatggtgag atcggaatca tcttgaatct gacaccaagc tatccgagag atgaaacgaa
     7861 cccagaagat gtaaaagcgg cgaagatcgc tgacgcattc ttcaaccgat cattccttga
     7921 ccctgcagtg aaaggagagt ttccacaaga gttgatcgaa attgtcaaag aattggctat
     7981 aatgccagag atccatgaag gagacctaga actcatccgt gaaaacacga ttgacctgct
     8041 aggaatcaat tactatcagc ctcgtcgaat caaagcaaaa gaaactccaa tcaatcacga
     8101 acatagcccc atgccagatg attattttga taattacgac atgcccaata aaaaaatgaa
     8161 tccatatcgt ggatgggaaa tttacgaaaa aggaatctat gatattttaa cgaatacaag
     8221 agaaaactat ggtaatatca aatgctatat ttctgaaaat gggatggggg tcgaaggaga
     8281 agaacgattt gttaacagcg aaggaattat tgaagatgat taccggatcg agtttgttac
     8341 ggatcatttg aaatacgtcc atcaagcaat ccaagaagga accaattgtg tgggttacca
     8401 catgtggaca tgtatggaca actggtcttg gacaaatgct tataagaatc gctatggctt
     8461 tatcgctgtt gatttggcac aagacggtaa acggacgatc aaaaaatcag gtcgctggtt
     8521 taaaacggtc tctgaaaaaa acggatttga tgcctgatat tttttgataa aacgttcaca
     8581 aaatgtttgt tctaacactg taatcgtgct acactaacag tgaaggaggc gtttacaatg
     8641 gtagatactt atcgtaatat tttagtagca attgatggtt ctgaaaaggc agaaaaagca
     8701 tttcttgagg caatcacgat tgccaaacga aatcaggcaa cgttgcacat cctgtatgtc
     8761 aatgaagtaa caggcaatta ctttggtgat tttgcatttg ttacaacaag ccttcaggaa
     8821 gaattggatg aggttgcgga aaaccagatg aaagaacatc gaaacttggc aattgaaaaa
     8881 ggcctgacag atatcgagac atatgttctt tacggttatc caaagacact gattgctaat
     8941 ttccatgaat cgaaagaacc aatcgatctg attgtcatgg gggcgacagg tctgaatgcg
     9001 gtagaacgtg cactagtagg ttccactact tcctatgtag tcaaccacgc atcatgtaac
     9061 gtcatggttg taaaataaat aaattaaggg aacagaaagc aaaaaagcat aacgcttctt
     9121 ttgctttctg ttccttttat ttttttgtaa aaaattactt tacctgatga agtaatcatg
     9181 ctattctaat tacaacgaca gatggagtaa tatggaggaa agaaaatgat ttcaagtgat
     9241 ggaatacgcg gatacaatga tgcgattatt ttatcgatcc ttaacaaaaa cgattcttat
     9301 ggatatgaaa tttctaaaac gattcgtgag aggacaaatg attcctatgt gatcaaagag
     9361 acaacacttt actccgtttt tgcccgttta gaaaagaatc aactgattgt ttcgtatcct
     9421 ggagaagtga ctcatggaaa aaagcggaca tattataaaa tcactgaaat agggcgtaat
     9481 cttttagaag aaaaaaagca agagtggctg acgacgaaag acgtagtcga tcaatttttg
     9541 gagggaaatt aaatggatac aatccgtaat tatttagaca gtctttttat cggagtacct
     9601 caaagtaccg agatagataa gctgaaaact gatttgttgg caaatatgga agaccattat
     9661 catgaattga tgggagaagg aaaaaatgaa caggaggcca tcggtacagt aatttcgaca
     9721 tttggttcaa tcgatgaatt attagaagaa ctagatgtcg aaaaaaaaca tcaagctgac
     9781 gagacggaaa caaacacagc ttctatttat ttaagcgaag cagaaaacta ctggaaagaa
     9841 tacagagcgg caagtctcca agtggcatca ggtgttctat tcattagctt atcttttgca
     9901 agttttcttt tcttttgttc agcaggatat gtgttcatgg gtatttcttg tctgattttc
     9961 ggtatagcat tagctgtcgg tttttttatt gcttctggta tgaaaatcac acgtttaaat
    10021 cactttttgc accatcggaa aatacctgaa aaagtattag cagaagcaaa agaaaaagaa
    10081 gaagagtatc agcgaagttt tggcttctca ctgattgcag gaattggatt atgtattttt
    10141 tcacttttcc cacttttggc ttctttaatg tggtatatgg atggtagtat cggtgcctct
    10201 atattttttg ttacggtagg aacgggtgtc ttcttgatca tttatggcag tctagtccgt
    10261 cattcttatc gtcaattcac tcaaagcgct tattactggt gaatgtattc agatttgtaa
    10321 aaatattcta tttatccggc gtagaaaaaa agactgtgac acgcttttgt cacagtcttt
    10381 tttatatata ataatttaaa aattcatcat ctaaatccgt agaattttct agattgctta
    10441 cccatgtaaa atcgttatag acagatttca gttgacttct gcgccattgt acagggataa
    10501 aaagacgcat gctatcttca ttgaatccga ctaataattt ctttccgtca gtaatgatgg
    10561 gacgttgtaa caaagtaaaa tgcgagacga tcagatcgac aagctctgag gtcgtacatt
    10621 ggtcgatttg gtctttcaaa tttttataat caatcgagcg tttagctaat agatcgtcaa
    10681 atccgttttc tgttaatgac aataaataaa atatctcttt ccttgttaaa ggctgtttag
    10741 gtgtgaaaat tcgttctctg taaggcattt gatggtcttt cagccagttt gccatcagat
    10801 ggtaactatg tgtgcgagaa gaatagatcg taagcataaa aaaactcctt aaatagtcca
    10861 ttaatttgtt agatctgttg agataaataa taggtaagga gctgtgcata agcattgatc
    10921 aaaacaaccc aaacatcatt agaaataact ttctaaagtc ttaaacttgc gacgagaagc
    10981 aactagacta atagaaaaaa tgtcttagcc atttggcttc ctgtttgttt ttttgcttat
    11041 gctgcagctc ctcgggaaag ggttctaaca atttgaagaa ggttgatgat tgatctagtt
    11101 taaattgtgt gaaatttgaa atctggtgtt tttactagat gaatcaccta acaactatga
    11161 ttataactta tagaacttct gttttgcatt cggttttgtt atagttgtta taaagtttta
    11221 ttttattaca gaacattttt aattaataaa gaacagttag aagttaaaat tgctgattca
    11281 catagagttt atgacttctt ggaaattctt tttcaatcag ttttttcatc tgtttatgaa
    11341 attctttttt ttcagtaaac ataaaggttt gggtatcgta accttcttca tgtaggaaat
    11401 agattattcc tttttctgtg ttttgcgtat aagcttttat actcctgtct tttttgaaag
    11461 aacagatcgt atagccatct atttggtctg gtagtcgttt gattttcttc aaaatgactt
    11521 ttttatcctc aagtttcatt tttttcacct gactttattt ttttcatatg gaaatcatag
    11581 cgcctttggt tatatgaaaa caagaaggat actgaaatat atataaatca aatagtggtt
    11641 atttattatt agaatagcga atacaaataa acgaccacta ttttctgatt tattgcaaat
    11701 gttttttcaa ataaatttga ttaccaagaa aaggtagtgg tatatttact gagggatact
    11761 ttgaaaaaca tatgatagaa acagtaacag tattatgaaa aatcttttta taaaaatgga
    11821 ggaatcgatt tgatggaaga tcagtatgta caagcaatga cgatcgcagg atcagatagc
    11881 gatggaagcg cagggatgca agcagatatg catacattct ttacaagaaa tgtttacggt
    11941 gtttctgtca tgaccgcatg tgttgcaggt aattcttacg gaatcggagc aagtgtgaca
    12001 ttgccaacag attttatcga caaagaattt gaactgattg cgaaggactt tcaaatacgt
    12061 gcagctaaaa caggtatgct agcagattca aagttgattg agacagttgt aaaaaattac
    12121 caaaaatatg attttggacc attagtcgtg gatcccgtaa tcgtaacgaa acatggaaat
    12181 cttttactag aagaatctgc tttgcaaagc ttgaaagaaa agcttgtccc tttggccgaa
    12241 gtactgacac ctaattttta tgaagcagaa aagctgactg gacggacatt agcagctgaa
    12301 gaagattttg tgcaagcagc aaaagaactg caagatatgg gtgcaaaaaa cgttatgatc
    12361 aaaggacatc atgctaaaaa tgcgcagcaa gaaacagtca atgactacgt attgttcgaa
    12421 tcaggaaaat cattttggtt aaatgcacca ttttatccta ccgaccggat caacggaact
    12481 ggcgattcgc tatctgcatg tattgcagct gagttagcga aaggaaaaac agttgaacaa
    12541 gcgatccgat tggcaaaaga ctatgtaaac cgtgcaattc gtgaagaaat aaatgtaggg
    12601 cacaaatatg gccccatcaa tcattgggca gctggtgaat tagagtaaaa gaaagaagtt
    12661 gtgaaaaagt tgccctgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt
    12721 ttttaacttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga
    12781 ggtcgtgagc ctgccgtcca gctccgagtc acaaggaaca agttcaaaaa acagcttctc
    12841 atgtttgttg aatattgtga cttgtgaccg aggagttggc aggagaacac cgtttgttcg
    12901 gaagaaggga ctgtgacaag atttttgtca cagtcccttt aatcttagta tatttttctc
    12961 tacttgaata aaaaaattat aggattggag aaaataatcg agaaatttgt tgcttgaata
    13021 atagccagtt agacatttcc ttgactgttt cagttgtcat ggatgaacaa tgtgtctcat
    13081 cttctttgaa ctgagtagca agttcttcta aaaactcagg atcgtaaatg acagcacttg
    13141 tttcaaaatt caatcggtag ctacgaatat cttgatttgc tgaacctacc atgcagattt
    13201 catcatccat tatcaatgtt tttgcatgga ggaagccgtt ctcatagaca aggatttgta
    13261 cattttcctt gatcagctgc cgagcataat attgtgttgc tcgataaata aaaggatgat
    13321 ccggtttgtt gggaatcatg attttcacat ctacaccaga ggcagttgcg atttttaaag
    13381 cagcaatgac actttcatca ggaacaaggt aaggtgtctg tatccaaaca cgttttttag
    13441 cagaagtaat caatttgata aatgaaagct tgatttgttc cctttcgtta ttcggtccgc
    13501 ttgaaacaag ctggatggat gtatcagcaa gcttagattc atctcgatct gcttttttaa
    13561 agaaataatt caaatgatag ccgacctttt tttcttcagg taccgagacg ttccagtctg
    13621 ttaagaagcg aagctggagc aaagaagaag cggcaccgaa aatccgtata tgcgtatcgc
    13681 gccaatagcc gaattttttt gttgtattta catattgatt ggcaatattg aaaccaccgg
    13741 tataactaat ctttccatcg atcacaacga tttttcggtg atcatggtaa ttcaatcgga
    13801 aacgaagtaa tgccctttgt gaggtaacaa atgtatggac atgtccacca tttttgatta
    13861 gacgattgaa atcttttgct tttgtgccat gagagccaaa tgcatcatat aatattcgaa
    13921 cttccacgcc ttctgcggct ttttcttcta aaacatgtaa gacttgctgg ccgatattat
    13981 ctgtcacaaa tgcgtaatat tcgatatgga tcgaatgttt ggcttttttt agatcttgaa
    14041 gcagtgaatc caatttctct tgtccgtctg tgagaagagt gacagaattc attcttgtca
    14101 gcggcatacg atttaatgaa gagaagaagt caacaaattg ctgtttgtct ttatcgccca
    14161 tgtcaggatc gtaatgttca atggtatctc ctctaaaaga ctgaaagttt tctaattctt
    14221 tcaagtcact ttgtcggaga tagaattttt tcttatccgt taatccacgt ccgaaaaata
    14281 aatataatac aaagcccacc ccaggaaggg caaacaatac taagagccat gcccaaatgg
    14341 ctgctacatc tcggggtttg atcaaaatag ccaccaaagc aataagtgca tttaatagat
    14401 aaagggcggt tataatacta gataccacaa aaatgttcct ccttacatac aataacatta
    14461 gtatagcatg atagaagaaa gaagcctacc aaggtgaata tacttttcac tcctttcatg
    14521 aaaatgtagg taaatcgggc taaagtagtt gctttttgta ggttaataag gtaggatatt
    14581 agtacagttg aatatctcct taggggagta accggcaacg tttgttgcga taatatcaac
    14641 agtaagttaa ccaaatggtt ttctggtatt gtatgaaatg gtgagaccta agacaaatgc
    14701 gtatttgtct taggtctctt ttttttcaga caggactagg gccaagatca aaaaatgggg
    14761 gatgaaattt ggaaaacaag gaacaagcac aacaagcggt ctataaagaa acgatcgact
    14821 cgttgatgca gcaaacgcag agctcaaccg atggattgag caccacgcaa gcgaaggaaa
    14881 aattagaaag agatggcttg aaccagctaa aagaaaagcc gaagaagcca acttggaaat
    14941 tgttcttaga aactttcaaa gatgcaatgg ttatcgtatt gctgattgtc gcactgattc
    15001 agatggtcat gggtgactac atcgaattct tagttatttt tgcagtattg atgttgaatt
    15061 ctataatcag tgttgtccaa acaaaaaaag cagaaggttc tttagaagcg ttgaaaaacc
    15121 tttcagcgcc cgatgctcga gtgattcgtg atggtaagga aaaaacgatt ccggcaaacg
    15181 aacttgtggt aggagatatc gtgttgttag acgcaggcga ttatgtacct gctgatggac
    15241 gtttactaga tgcgggatca ttaaaagtag atgaaggtat gctgacagga gagtcgactt
    15301 ctgcagaaaa agaagtaacg gatatcacaa gtacggtacc aatcggagat cgtacaaata
    15361 tggtattcag tggaacgatc gtaacttacg gacgcggtaa attcttagtg acttcaactg
    15421 ggaataatac ggagatcgga caagttgctg gccttttgga atcaacaact gagcaagtca
    15481 ctccgttaca acgaggtctg gataaattca gtaaaaaact aagtttggct attttggcat
    15541 tgtctttatt gattttaggt atccaactgt ttcgtattta tcttggagaa ggtacaggag
    15601 atatgactgc agatatcgtc agcgcagtga tgtttgccgt agccgtagcc gtagctgcta
    15661 ttccagaagc attacagtct atcgtaacaa tcgtgttatc tttgggaacg aataaaatgg
    15721 caaaacgtca tgccattatt cggaagcttc cagcagttga aacacttggt tcgacaagta
    15781 tcatctgtac agataagact ggtacattga cacaaaacaa aatgacagtt gttgactact
    15841 ttttaggtaa tggcgatact ggggatttca caaatgaccc ttcaaaatgg acagctgacg
    15901 aacgtcgctt gatcgaaatc agtgttttag cgaatgatgc atcgatcagt gaagacggta
    15961 ctaaattggg cgaccctacg gaagtagcgt ttatcgactt tagtgaaaaa ttaaaccagc
    16021 cttatcagga aatccgaaac aactacccaa gacaagcaga acttcctttt gactcagatc
    16081 gaaaattaat gtctactgga cacacaattg ataacaaaaa ttacttgttt gttaaaggcg
    16141 gaccagatat cgtcttccaa cgcagtaaaa aagtattgct aaatggtgaa gtcgttccga
    16201 tgacggatga attgatgaaa aaattccaag agcaaaatga agctttttca acacgcgcat
    16261 tacgtgtact agcttttgct taccgtccac ttgataattt tgatttatca tttgaagatg
    16321 aagacgattt gatccttgtt ggtttgcttg ctatgatcga tcctccacgt gctgaagtga
    16381 ctcaggcggt aacagaagca agaagtgcag gaatcaaaac aatcatgatt actggtgacc
    16441 acaaaacaac agctgttgct atcgcaaaac aaatcgggat ttttgaagaa ggaaacctgg
    16501 ctttgaccgg gacggaacta gatgaattga cagaagatca actaaacgaa gttttagaaa
    16561 aagtcactgt ttatgcacgt gtttcacctg agaataaaat tcgtatcgtt cgtgcttggc
    16621 aaaataaagg tcatatctca gctatgacag gtgatggtgt aaatgacgca ccagcattga
    16681 aacaagcaga catcggaatt gctatgggaa cgggtacaga tgtggcaaaa gatgcagcag
    16741 cgatggtatt gacggatgat aactttgctt ctattgtcag cgcagtagaa gtaggacgta
    16801 atgtgtttga taatatcaag aaagctattt cttatctatt tgcaggaaat ttaggtgcga
    16861 tcattgcgat catctttgcc ttagttgctg actggtccaa tccatttaca gcattacaac
    16921 tgttgttcat caatttagtc aatgattcac tgccagcaat tgcattaggt acagaaaaag
    16981 ctgaacctgc tacgatgaac cgcccgccaa gagatccaaa ttcaggaatc tttacggaca
    17041 aaacattgat ttctgtaacg tatcgtggtg tgctgattgg gattgctgtt atctttgctc
    17101 aatggcttgg catgcagcat tcacctgaat tcggtgtagc catgtcattt tcaacattga
    17161 tttggtgtcg gacattgcaa acattcccag cacgttcaaa cacacaaaca gctatccaag
    17221 cagggttctt ctctaacaga agtgtcttac tagctgtagt tgtatgtagc gcgctttact
    17281 gcttaacaat gatcccagga ctgaaagaag tcttctctat cccatcagca tttggattga
    17341 cagaagtctt tacatgtatg ggaatcgcac ttgtgacaat tgttttgatg gaaatcacta
    17401 aattgatcat tgtacgagta caaggaaata aataaaacga atcaacgagg ttgtgagcct
    17461 gccgtttaat tctgagtcac aaggtgcaat ttcaacaaac agcttctcat gtttgttgaa
    17521 tattgtgact tgtgaccgaa gagttggcag gagaacaccg tttaaggaag aagttgtaaa
    17581 atcagcattt tgagctaata ctgagggtca tctgattgga cactgtttat tcggaaagag
    17641 ggactttgac acgactttgg tcacagtccc tttttttatt tatgatagac ggaaagccaa
    17701 tcattttatt attcatcaaa agagatagaa tagataaaag aggagaatga tttattccga
    17761 aaagtgaaaa catataacaa aaaattagag gaaatcaaat gaatgaaaaa gaatgggatg
    17821 aatttgctga agaatattat gaaatccagc aagaatcgca agtaactatt gctgaagatg
    17881 taaaaaatta tctgaaaacg gagaaagtcc tgcctgctga aacagcagca gacgttgctg
    17941 gaggatctgg cagatttctt tccttagcaa aagaaattaa tcgctatgat ttgatcgatt
    18001 tttcttcagg aatgctagaa aatgcaaaaa aagaagcaga aaaaaatgag ttgccaaatg
    18061 tttcctttat caaacagtcg ttagaagaat ttatccaatc aaatgagaca tatgaaatgg
    18121 tttttacagc agcaaatcca gcattggtac atcttattca attagaaaaa ttgatgaaaa
    18181 agtcaagcaa atggtgcgtg ataatcagag tagtggaatc aatcgatgat gttttttatc
    18241 ctatcgaaaa gcaactagga atgatagaag aagatcctaa tacatcgcca gttgtaatgg
    18301 atcaatttga aacatactta attgatgaag gattcaaagt gaagaaaaaa gattttactt
    18361 attttacaca ggaatcaatt actgaagaat tgatgaagct atactatgac gcgtacgcaa
    18421 aagatcctgt tgtgaccagg tacattcgca aactattcag tgaaaaacga gaaatcctta
    18481 gtacgactaa gctgacctat agactgctcc tttggtccgc agataaaaaa agaacctttc
    18541 acgactcgtg aagggttctt tttatgattt tattatattt tgtagtaacg aatatcattt
    18601 tcgatctttg taatcgaaaa aggtacatct gaagtatatt ctgcacattc tccgtgttca
    18661 ggaagcgtct cactgaaaat cgtttcatat tcgtcaacag tgatccgtgt tcgagaatct
    18721 agcatctctt gatgagattg tgcgaagaga tattcttgat agttttcttc cagataacct
    18781 gtaaaaaatt cacttactgc accagaaccg tagctgaata gaccgatgcg atctcctggc
    18841 tgtagtgatt tcgaattttc cagtagagaa gttagcccca ggtataatga accagtgtaa
    18901 agattaccga ttcgtcggct gtaacggatg ctttcttcat agcgagccat aagacgttcc
    18961 tgattttctt cgtctgtttg gtctaatacg ctttgcaatg ccttttttcc catcttagta
    19021 tacggaatat ggaaagcaat cgcctgataa tcttcgagtc ctcgacccga caattcttta
    19081 tgtcgattcc aaactttttg gaatgattcg atatacgtag aattagataa aggaccatca
    19141 acaacaggaa attcgctata atctggacgc cagaaatcat agatatcttc tgtcagaaat
    19201 acgctgtcgt cttcaatcga taaaatacgc gggttttgag tgatcatcat cgcaacagca
    19261 ccgacacctt gcgtcacttc accaccgctt gccaagctgt aacgagcaat atcacttgct
    19321 atgactagta cttttcgttc tggatgattt ttgacatatt ctttcgccat gtgcaaagct
    19381 gcggtgccac cgtagcaagc ctcttttact tcaaaagaac gagcaaaaga ttggattttc
    19441 agtaaatggt ggataattac cgcactcgct tttgaatgat caatcccgga ttctgttccg
    19501 acgatgacca tgtcgattag ctcacggtct ttttctgtta cgattttgct ggcagcattt
    19561 gctcctagtg taatgatgtc ttcatttgca cgattgactg ccatttggtc ttgaccaatc
    19621 cctatatggt attttgcagg atcatcccca cggctttctg ccaactctgt catatctaaa
    19681 tataaattag gaataaaaaa ggaaagacga tcaatcccta ttttcatttc aacacctctt
    19741 tacttatgat aaaaaactta ctttactaag gtatcataaa aagattaaaa gaaatataaa
    19801 aaagagaata tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag
    19861 ctatttgttt ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa
    19921 gtcgttatga tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca
    19981 tttactgcag ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg
    20041 aaaaaagata aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa
    20101 aatgttgcca gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg
    20161 atcaatgaag tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa
    20221 ttaggtgaag cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg
    20281 ctgaaacagt atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac
    20341 gacggattga ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca
    20401 acacaatttt ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa
    20461 gcagcacatg ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct
    20521 gatgaagacg tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg
    20581 ggcacgttac gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg
    20641 ttgaatgacg gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat
    20701 ctgccttatt tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg
    20761 ggaatcgctc caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca
    20821 acaatcgatc tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa
    20881 gaactgcagt tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat
    20941 ccaattggtg caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa
    21001 cagaagagat acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg
    21061 ttagaagcga atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt
    21121 acgccatctg aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg
    21181 ttgatttttc aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa
    21241 gtgagtgaag tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa
    21301 aaatggattc cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca
    21361 aaaatctgtg gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt
    21421 ctatcaggaa aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag
    21481 ctgattcttt gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa
    21541 gatatctcta ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta
    21601 gtggatgtaa aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca
    21661 aacaagttac gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt
    21721 gctacagaat cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac
    21781 aaagaaatcg gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt
    21841 gacccttacc gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg
    21901 gcagctacag gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga
    21961 aatggattat accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa
    22021 ctaacggtgc ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca
    22081 aaagcatctt tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct
    22141 gcagtagggt tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa
    22201 aaaggccaca tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag
    22261 gaaatcgagc aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct
    22321 atacaaatat tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag
    22381 caagatttac agttatttta tttactgcag atcttgcttt tttggctctt cgtcaaattg
    22441 tgttggcgaa gacaaatttg aataaaactt agtccgatat gcggcgcgtt ctgtgcgccg
    22501 catatttttt tagttttcaa agatcagacc ttgttgtaaa tagatccgtg cccgaaagtt
    22561 ataaaagttg cgataaccaa acgcaattcg tttaatcagc ttgatcttgt tattcagccc
    22621 ctcagtgacg ccgtttgagt ggggcacctt aaaggcattt gaaatacccg attggtagcg
    22681 atggaagatc gtgaatttct ttttaaacga ctgaggtaac gtagatgaaa ttgttttaag
    22741 ggtttcttga aagccttccg tatctcgttt aacgaaataa tagcgtaata attgacaaaa
    22801 atgatacgca gttcgcaatt cttcgttgta gcttaacagc tcatcaacga tttctgtttg
    22861 actaattggt cgtttaaaca gtcgatgata atgccgtttg agtggctcca atgtcgtgga
    22921 atctttgagt aagagtttcc aatatcgctt gagccgacgg tacttcttct gattttcaga
    22981 gtgtgaggtt cgaaaacgat tcatgatctg gacacgtaat tgattgaagc tgcgattgat
    23041 gtgttggaca atatgaaagc ggtcagttac gatctccgca tttggaaaga ctgttttaag
    23101 caactgacaa tagctggcat tcatgtccat cactaggtac ttgaccttta aacgggcctt
    23161 tctaggaaag cgcaggaagt attgagttag tttgtaaagt ctgcgatctt caaggatcgt
    23221 aaatagctga ttggttgtcc catcaacaca aataaaactc atggaaccag aaacagactt
    23281 cattgatttg aactcatcca tacacaacac cgtcggaagg aagttcatac gtggctggta
    23341 atgcttcgtt gcttcacgca agatccgtgt caccgtccca tccgagacga agtacttctt
    23401 agcgattagt ttacgtgatt gattttcgta cagttccatg agaatcgcct gcttcagttc
    23461 ctttgttagt tgatgatgtt catcaactaa gtccgttttc gcagaaaagg tccgtccaca
    23521 atcatggcag agaaaacggg tacgttttaa ttctaaaata gtcaactttt ctttgacttt
    23581 caacatctga gtgcgtgtcg tataggaacc gttgcggata atacgaatcg atccacagtg
    23641 ttcacaatgg gaaggtgagt catccaatct ccctttaata aactgcgctt gaacgccttt
    23701 atattctttt tcaatcagcc attcttcatc aaaaaataaa tgtttatctg ttaatccgag
    23761 taattttcta gtatgattat ccattgagag actcctttgg tagggtgacg ttttggtcga
    23821 ctaaatttta ccaaaagtga gtctcttttt gagttgaaac gaagaaaaaa gtgttagtag
    23881 aaaatcgctt tcgattttcc accaacacaa aatattatag aaccataaaa atttcaggac
    23941 aaatacacat agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc
    24001 gaaagtcttt taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt
    24061 tcattacgag ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg
    24121 agaaatgatt gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata
    24181 gttctctacg tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc
    24241 aatagatact ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa
    24301 agaaatgttg tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac
    24361 tgcttttgat cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac
    24421 cgcgcgacat ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg
    24481 agcaaaatat ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga
    24541 aaaaagtttc ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct
    24601 tcattgtcat gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc
    24661 attcgataaa cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc
    24721 gctttttgtt tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct
    24781 gtatccaaac tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg
    24841 cgagcaattg ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta
    24901 agtgactgga tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt
    24961 gaagtaactt ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta
    25021 gagctttgga gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga
    25081 agctcagtta gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct
    25141 cctgaaatgg taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat
    25201 tctccttcct ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa
    25261 ccaaatgttt tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta
    25321 ttgctgtaat tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg
    25381 cgacaatgat caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca
    25441 tcgcgcttgg taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta
    25501 ccaccccaac aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc
    25561 cttgtgcttt cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa
    25621 taccgaccac ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat
    25681 tttgggcaga atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac
    25741 ccttcgatgc tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg
    25801 gctgttcttc ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta
    25861 gggacgcata gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg
    25921 tcaaaggatc gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag
    25981 ccagattttg agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt
    26041 ttggtaataa aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc
    26101 ctttgattgc tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca
    26161 gatctcctct tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa
    26221 tctcttgtgc gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta
    26281 gtcctgacaa catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat
    26341 atcgtaattt agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat
    26401 gatagattga tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt
    26461 gtcacttgtg ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct
    26521 taacatgagt cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag
    26581 tgaccaaatc aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta
    26641 ttatccaata atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat
    26701 ggtagtgaag gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg
    26761 atgatgccgg aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag
    26821 actccagtat tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa
    26881 cgaggtgcag atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg
    26941 aaagcattat tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat
    27001 atcagttgtg atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa
    27061 ggaaaagagt ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa
    27121 gagcagatat ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa
    27181 gtttatatga gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa
    27241 accttacgta atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac
    27301 acgaaagcaa cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt
    27361 tcttttggga ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct
    27421 gtcattagag aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca
    27481 gcaaggcgat ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca
    27541 gccattaatg gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa
    27601 tggtgagata ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa
    27661 aatggatacg aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc
    27721 atctgccctc tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc
    27781 ctacatccaa attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac
    27841 gattatgatt ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt
    27901 gtctagtcta agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga
    27961 gaatgcttct catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct
    28021 ttttaccaag cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc
    28081 agagacgagg cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc
    28141 tgatcagcag ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt
    28201 taaaccattt caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc
    28261 aacattagaa gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga
    28321 taatgcatta aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca
    28381 taaagaatgg ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc
    28441 aaggattttt gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata
    28501 cgggcttggc ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt
    28561 atccaattat cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa
    28621 ataatgctta tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg
    28681 cgaataaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg
    28741 accacttctg tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg
    28801 tgtacatcac cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt
    28861 tcaacatctt catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg
    28921 cttacatcta cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg
    28981 tctgttccct gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt
    29041 ttgaccggta cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct
    29101 ccagttgtca catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca
    29161 tcatctagat ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat tgtatcagtc
    29221 tcgccaggta cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt
    29281 tgaaagcctg tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca
    29341 gtccccattg tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt
    29401 tctggctgac gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta
    29461 tattgatata aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa
    29521 aaagtatctt ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca
    29581 tctgtttcaa catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca
    29641 gtagtataaa taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact
    29701 gataatgctt cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg
    29761 taaattcctc ctagcaaata gatttatgat tggtatattg ttacttataa atttattcta
    29821 taggagacga ttgttttcta caaatattta gattggaatt tgattaaaac aatttaatt
//
LOCUS       DASNNU010000029        28641 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.029, whole genome shotgun sequence.
ACCESSION   DASNNU010000029 DASNNU010000000
VERSION     DASNNU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.029"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            347..1957
                     /locus_tag="U8116_001191"
     CDS             347..1957
                     /locus_tag="U8116_001191"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEN8434199.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2249..3118
                     /locus_tag="U8116_001192"
     CDS             2249..3118
                     /locus_tag="U8116_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEN8434200.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3283..4554
                     /locus_tag="U8116_001193"
     CDS             3283..4554
                     /locus_tag="U8116_001193"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN8434201.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4551..5846
                     /gene="rho"
                     /locus_tag="U8116_001194"
     CDS             4551..5846
                     /gene="rho"
                     /locus_tag="U8116_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEN8434202.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            5945..6208
                     /locus_tag="U8116_001195"
     CDS             5945..6208
                     /locus_tag="U8116_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEN8434203.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6653..7102
                     /locus_tag="U8116_001196"
     CDS             6653..7102
                     /locus_tag="U8116_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN8434204.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7159..8577
                     /locus_tag="U8116_001197"
     CDS             7159..8577
                     /locus_tag="U8116_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN8434205.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8577..9437
                     /locus_tag="U8116_001198"
     CDS             8577..9437
                     /locus_tag="U8116_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEN8434206.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9611..10384
                     /locus_tag="U8116_001199"
     CDS             9611..10384
                     /locus_tag="U8116_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN8434207.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10531..10896
                     /locus_tag="U8116_001200"
     CDS             10531..10896
                     /locus_tag="U8116_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEN8434208.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11044..12252)
                     /locus_tag="U8116_001201"
     CDS             complement(11044..12252)
                     /locus_tag="U8116_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN8434209.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12271..12549)
                     /locus_tag="U8116_001202"
     CDS             complement(12271..12549)
                     /locus_tag="U8116_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434210.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            12703..13398
                     /locus_tag="U8116_001203"
     CDS             12703..13398
                     /locus_tag="U8116_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN8434211.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(13840..14637)
                     /locus_tag="U8116_001204"
     CDS             complement(13840..14637)
                     /locus_tag="U8116_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEN8434212.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14634..15818)
                     /locus_tag="U8116_001205"
     CDS             complement(14634..15818)
                     /locus_tag="U8116_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN8434213.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(15843..17090)
                     /locus_tag="U8116_001206"
     CDS             complement(15843..17090)
                     /locus_tag="U8116_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN8434214.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17305..19050
                     /gene="dnaX"
                     /locus_tag="U8116_001207"
     CDS             17305..19050
                     /gene="dnaX"
                     /locus_tag="U8116_001207"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEN8434215.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19070..19384
                     /locus_tag="U8116_001208"
     CDS             19070..19384
                     /locus_tag="U8116_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEN8434216.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19409..20005
                     /gene="recR"
                     /locus_tag="U8116_001209"
     CDS             19409..20005
                     /gene="recR"
                     /locus_tag="U8116_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEN8434217.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20069..20713
                     /locus_tag="U8116_001210"
     CDS             20069..20713
                     /locus_tag="U8116_001210"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEN8434218.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            20728..21057
                     /locus_tag="U8116_001211"
     CDS             20728..21057
                     /locus_tag="U8116_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEN8434219.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21071..22009
                     /gene="holB"
                     /locus_tag="U8116_001212"
     CDS             21071..22009
                     /gene="holB"
                     /locus_tag="U8116_001212"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEN8434220.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22045..22869
                     /locus_tag="U8116_001213"
     CDS             22045..22869
                     /locus_tag="U8116_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEN8434221.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            22862..23209
                     /locus_tag="U8116_001214"
     CDS             22862..23209
                     /locus_tag="U8116_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEN8434222.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23278..24150
                     /gene="rsmI"
                     /locus_tag="U8116_001215"
     CDS             23278..24150
                     /gene="rsmI"
                     /locus_tag="U8116_001215"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEN8434223.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24259..25380
                     /gene="dinB"
                     /locus_tag="U8116_001216"
     CDS             24259..25380
                     /gene="dinB"
                     /locus_tag="U8116_001216"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEN8434224.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25434..26036)
                     /gene="nrdG"
                     /locus_tag="U8116_001217"
     CDS             complement(25434..26036)
                     /gene="nrdG"
                     /locus_tag="U8116_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEN8434225.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26351..28540)
                     /gene="nrdD"
                     /locus_tag="U8116_001218"
     CDS             complement(26351..28540)
                     /gene="nrdD"
                     /locus_tag="U8116_001218"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEN8434226.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT         9203 a         5180 c         6027 g         8231 t
ORIGIN      
        1 tatttcactt aaaatttttc agtaacgatt ttctgatttg cttaggttta acgttagcta
       61 tgtttatatc aagcttatta tgtgggttaa attttttttg cactcataaa ggacttaaga
      121 aataatttct caaaggatat cgaaaaagaa aaaattgtaa gcggatagta ttcatttgag
      181 tacttgactt tcccttagtc agcttgtatt atacaccttg ggcacccttt tagagggtca
      241 gaagtttaca gaaatttcgg ctccctattc ttattgaaga atagggagct ttgtttattg
      301 tctgagactt ttcctgcaca taaattaaaa aggagttttt gaacttatga caaaatatat
      361 ttttgtgacc ggcggtgtcg tttcatcaat cggaaaaggg attgttgcag cttctttagg
      421 acgcttattg aaaaaccgcg gcttaaaagt aactatccaa aaatttgatc catacatcaa
      481 cgtggatccg gggacgatga gcccatatca gcacggagaa gtattcgtaa cagatgatgg
      541 tgctgaaaca gatttggatc tgggccacta cgaacgtttt attgacatca atttgaacaa
      601 atactccaac gtgacgacag gaaaaatcta ttctgaagtt ttgcgtaaag aacgtaaggg
      661 cgaatacttg ggagcaactg ttcaagttat tcctcatatt acaaatgaga tcaaagaaaa
      721 aatcatgcga gcagctaaaa tgactgattc agatgtgatc attacagaag tagggggaac
      781 agtaggggat atagaatctt tgccattcct tgaagcactt cgtcaaatga aagcagatgt
      841 cggtacagat aatgttatgt atatccatac aacattgatt ccttacttga aagctgctgg
      901 agaaatgaaa actaaaccaa cgcagcacag tgtaaaagag ctacgtggtt taggtatcca
      961 accgaatatt ttggtggtac gtacagagca acctgtttct caaagcgtga aaaacaaatt
     1021 ggcacaattt tgtgatgtcg aaccagaagc agttatcgag tcgccagatg tagatacact
     1081 ttactccatc ccattagttt tacaagcaca aggaatggat caaattgttt gcgaccattt
     1141 aaaactagat acaccagaag cagacatgac agaatggaaa gaattagaag aacgtgtctt
     1201 gaacttgaag aaaaaagtac ggattgcgct tgtcggtaaa tatgtcgagt taccagatgc
     1261 atatatctca gtagttgaag cattgaaaca ttctggtttc gcctataatt cagacatcga
     1321 gatcgattgg atcaaagcac aagaattaac gagagaaaat gtagaagaac gtttgaaaga
     1381 cgcagatgga atcttggttc ctggtggatt tggtgatcgc ggagtagaag ggaaaatcga
     1441 agcgattcgt tatgctcgcg aaaatgacgt tccgttttta ggtatttgtc ttggtatgca
     1501 gatggcttgt gtcgaatttg cacgtaacgt cgttggatta gaagatgcgt catcagctga
     1561 aacgaatcca gatacagcaa acaatattat tgatttaatg gctgatcagg aaaacatcga
     1621 gaatttgggt ggaactttac gccttggatt atacccatgt aaaatcaaaa aaggaacgaa
     1681 gacggctgaa gcatatgatg gagccgatgt tgtgcaagaa cgtcaccgtc accgctatga
     1741 gttcaataac aaatatcgtc aattgtttga agaacaaggg ctagtctttt caggtgtctc
     1801 accagataac cgtttagtag aaatcgtaga gctgccagag aaaaaattct ttgtgggatg
     1861 ccaattccac ccagaattga tttctcgtcc aaatcgcccg caaaaattaa tcaaagcatt
     1921 tgtcggagca tcattagcag atcacgaaac aaaataattt ttgaacttga tagaaagaga
     1981 aaagtagcaa gagaagggga taagcaattt tcttgtcact tttctctttt tctgatttgt
     2041 tattatttgt aggaaaaaag atagaaagca aaagactttt ttcgattaaa gcgctattgg
     2101 atattttatt ttttgcaaaa aacttcttga aaattttcat tttcattgat tttagtagaa
     2161 aactaccgaa atgtttggta aaatagggtc gttggttaag aattgtctta acaaatttag
     2221 ttttacaaaa cttaggagga attttattat gccagtagta tcaggagctg aatttttaaa
     2281 agctgctcgt aaaggcggtt acgctgtcgg cggattcaac acaaacaact tggaatggac
     2341 tcaagcaatc cttgaagcgg ctgaagcaaa acaagcacca gtacttatcc aaacatcaat
     2401 gggtgcagct aaatacatgg gtggttacaa aatctgtaaa gatttaatca ctgatttagt
     2461 tgaagcaatg aacatcactg ttccagttgc tatccactta gaccatggtg attacgaagc
     2521 tgctttggaa tgtatcgaat taggctatac ttcagtaatg ttcgatggtt cacatctacc
     2581 atttgaagaa aacttgaaat tagctaaaga cgttgttgaa aaagcacatg caaaaggtat
     2641 ctcagttgaa tgtgaagttg gttcaatcgg cggagaagaa gacggaatca tcggtaacgg
     2701 cgaattagct gatattgaag aatgcaaaca aatggttgct acaggtatcg actacttagc
     2761 ttgcggtatc ggtaacatcc atggtcaata tccagaaaac tggaaaggat tagcttttga
     2821 ccacttacaa gcaattgctg atgctgtagg tccagacatg cctttagtat tacatggcgg
     2881 atcaggtatt cctcaagatc aaatcgaaaa agctatctca atgggtatct caaaagtaaa
     2941 tgttaacact gaattccaat tatcatttgc tgcagctact cgtaaatata tcgaagctgg
     3001 taaagataaa gaaggtaaag gattcgaccc tcgtaaatta ttagctccag gtaaagcagc
     3061 aatcgttgaa gatgctaaat ctcatatcga ctggttcggt tcagctaaca aagcgtaagc
     3121 atccacataa cagatacgat atataggaat ggagaacgac ttttgaagtc gctctccatt
     3181 cttttttact taaaacggca gaccatgctc gtttcttttt tttatctatg ataaaataca
     3241 agcatatcca tgaaaaaaga ttaataaaca aggtggaaac agatgaaaaa aatcgtaatc
     3301 aaaggaaacc gacctttagc tggtgaagta acgatcagcg gtgcaaaaaa tagtgcagtc
     3361 gcattgattc cagctgcaat cttagctgat tccccagtga tcttggaagg tgtgccggac
     3421 atacaagatg ttcattcatt aattgaaatt ttagagatca tgggcgcaac tgtccatttt
     3481 tccaacaaca tactagaaat cgatcctcga gaagtcgtat cgattccgat gccgcacgga
     3541 aaaatcaata gtttgcgtgc ttcttattat tttatgggaa cactgcttgg taaatttggc
     3601 gaagcggtag taggcctgcc aggtggctgt tatttaggcc ctagaccgat tgacctacac
     3661 gtcaaaggat ttgaagctct aggtgctgaa gtgacaaatg aacatggtgc catgtattta
     3721 cggacagaaa ataaaggatt gcgtggcaat cggattttca tggacgtggt ttcagtcgga
     3781 gcaacgatca atgtgatgtt agctgctgta aaagcaaaag gacagacagt aattgaaaac
     3841 gctgcacgag aaccggaaat catagatgtg gcaactttac tgaataatat gggggcaaaa
     3901 gttcgaggag cgggtacaga tgtgatccgt atcgaaggag tcgaaacatt acatggctgt
     3961 cggcatttca tgattcctga ccgcatcgaa gcaggaacct atctagcatt agcagctgca
     4021 gtaggaaatg gtatcaaagt gaaaaatgtg atctttgaac atttggaaag ctttatagca
     4081 aaattgcagg aaattggtgt ccatatgaaa atcagcgaag atgaaatcga agtttacccc
     4141 tcgaaagaat taaaacccgc aaacatcatg acctatcctt atccaggatt tgccacagat
     4201 ctgcagcagc cacttactgc gttactattg atgactacag gcacttcaga aatcatcgat
     4261 acgatctatg cgaaacgtgt gaaccatgtg ccggaactag cacgaatggg cgctgatatt
     4321 accgtggaag gaaatatgat catcgtgaat ggtccaaata aacttcacgg cacagaagtc
     4381 gtggcttctg atcttcgggc aggtgcttgt ttagtgattg ctggactatt ggcagaaggg
     4441 acaacgacga tttacaatgt agattatatc ttacgcggtt atgatcatat tatcgagaaa
     4501 ctaacagcct taggggcatc aattgaaatg atagaggaag tgggagctga atgagcgatt
     4561 atttgacaat ggcagaacta gagaacaaaa cgttaaaaga agtttatagt tatgccaaag
     4621 aatttaaaat tccttattac agtaagatga ataaaaagga attatctcta gctgtgattc
     4681 gtgcacaagc agagaaacaa ggattttatt atatggaagg aatattggat atcgttggtc
     4741 aagatggtta cggtttttta cggccaatca attatggacc aagtgcagaa gatatctata
     4801 tttctgcctc acaaatcaga cgattcagtt tgagaaatgg ggacaaagtg gcaggaaaag
     4861 cccgtccacc taaagaatcg gaacgttatt atggtttgat gcacgtggaa agcgttaatg
     4921 gaaaagatcc ggaagaagca aaagaacgtc cgcattttcc agccttaaca cctctttatc
     4981 cgcaaagaca gcttcaactg gaaacaacac cgcaaagact atctacccga atgatcgata
     5041 tcttttctcc aatcggtttt ggtcagcgtg gattgatcgt cgcaccgcca aaagcaggga
     5101 aaacttctgt attaaaagaa atcgcaaatg gtatcaccga aaactatccg gaagtagaat
     5161 taatcgttct actgattgat gaacgaccgg aagaagtaac ggatctagaa cgaagcgtga
     5221 aaggcgacgt ggtatcttct acatttgatc tgcagccgca aaaccatact cgagtttctg
     5281 aattagtatt ggaacgcgca atgcgtttag tcgaagataa acgagatgtc gttatcttga
     5341 tggatagtat tactagactt gctcgggcct ataacttagt cgttccacca agcggacgta
     5401 cattgagtgg cgggattgac ccagccgcat tgtataaacc taagaaattt ttcggggcag
     5461 caagaaacat cgaagaaggt ggcagtttga cgattttagc gaccgcttta gtcgatacag
     5521 gaagccgtat ggatgacgtg atctatgaag aattcaaagg aactggaaat ctagaattac
     5581 aactttcacg tgaattatct gaacgtcgga tcttcccagc catcgatatc aaaaaatcag
     5641 gtacccgaaa agaagaatta ttgatgccaa gtgaagaatt agaagaaatt tggaaacttc
     5701 gaaaaaatat gactggagat tcattagaat atacggaaca actgatccgc tttttgcgta
     5761 aaacaaaaaa caatcagcaa tttttcaaag aatttcaaga tgtctccttt ggtaaaaaaa
     5821 atccgaccag aaatctgaaa agataattgc aaccttgact gaaacatgtt aagatgttaa
     5881 tgttgtaaat cgacaaatca actctggttc agcaaatgga gcagggcaaa ggagcgaaaa
     5941 aatcatgaaa gaaaacatcc atccagatta ccacccagta gtattcatgg attcaacaac
     6001 tggtttcaaa ttcttgtcag gttcaacaaa aacttcacaa gaaacagttg aatgggaaga
     6061 cggtaataca tacccagtca tccgtgtcga agtaacttct gactcacatc cattctacac
     6121 tggacgtcaa aaattcacac aagcagacgg acgtgtggac cgtttcaaca aaaaatacgg
     6181 tctcaaagac gcaaacgctg cggaataata aaaacgctga taactcaacg tttagcagac
     6241 tatctgatag ggatagtctg tttttttatg ttttttgcca atttaaaact tcgtttgatg
     6301 ctgaaaaagt cgtaggatca taatgcacaa aacaatgaaa aactagtata tagttttaat
     6361 tatccacttg ataaaaacac cttatattaa cgaaatgaag aggggttatt tcgaagtaaa
     6421 aaaccattta cctaaaatta gaagctaagc tttcctttca aggaaggctt tttgttttgt
     6481 tatagacttg cgattgtttg tgagctagat cgttcatttt aatgacttat acacgcggat
     6541 gatgggtctt tttcctctgt tcattcataa tattgaacgg tacagctagc gatgctatac
     6601 tgacctcagt tccaagaaaa cgttaacatt ttttgtgggg gagaggtttt ttatgaggtt
     6661 tgatcaattg atcgcagaag aactgattgt tttcgatcca tcgatagatt caaaaggatc
     6721 attatttgag cgaatagggg atgtgttgaa aaaaacaaat aaggtaagaa atgttcaaaa
     6781 agtcgttaag gcactattca aacgagagga ggagatctcc acaggaatag aagatggatt
     6841 tgggattccc catgcaaaaa gcaaatatgt gacaaaacca gcactgtgtt tttttcatac
     6901 agccaaatta acggactata gcgggataga tgaaatgcca attgaatata tttttgccat
     6961 cgtagttcct gaaaaatcag cagatatgca tctagagatt ctcagcacac tctccagacg
     7021 attgatgaat gcagatttta gaaagggaat aaaagaagcc actacgccag cagatatcat
     7081 cagacagatt agtcaagaat gaaaatagta gagtctttgt ccgagtatcg atagtgaaga
     7141 aaaaggagga aaataatcat gaaaatcgtt ggtgtaactg cttgcccgac tggaattgct
     7201 catacttata tgtcggcaga aaaattagaa gtaacagcaa gaaatctaaa ccatcaagca
     7261 aaattcgaaa cacaaggtgt aaaaacagaa aatcagttat ctgaacaaga aatcaaagag
     7321 gcagatgcaa tcattcttgc agtagataaa gaaatcgaat tggaccgatt tgccgggaaa
     7381 aaagtaaagc gtgtctctac aagccgagca atcaaagaac cgcaagtagt aatagacgaa
     7441 gcattgagag atattggcgt attcgttgtg tctaatgaaa aagaaccagc tgcaaatgag
     7501 aaaaagccga caatctataa tcatttcatg aatggtgtga attacatgct tccatttgtg
     7561 atcgcaggcg gaatcatcat cgcactgagt tttgcctttg ggattacagc ggctgatccc
     7621 aactcagcag actataatgt gttagcagct gcattttcaa ggatcggagg agatacagcg
     7681 tttgccatga tggttcctgc gttaggcgcg gggattgcta catcgattgc tggaaaagca
     7741 ggttttgccc caggaattgt tgccggatta ctggcctcta caggtggttc aggttttctt
     7801 ggcggaatga ttggtggcgt gctagcaggg tatatctgtg attttctggc aaataaaatt
     7861 agcgtaaaaa aagaattttc tgctatttat cagctgattg tcgtcccctt tttatcgatc
     7921 ttaattattg ggcttttgat ggtatttgtg attgaacaac cgatcacatg ggtgttggat
     7981 gctttgacaa attggctgaa tagcttggga aatacatccg ggctattgtt cggattactt
     8041 atcgggatca tgatggcagc tgatatgggt gggccgatca ataaatcgac ttcgactttc
     8101 tcgatcggtt tgatgtcagc tggagtaaat gcaccgattg ctgcttgtat ggctgcagga
     8161 atgacacctc ccctaggtat tgctttagcc actttattat tcaaacataa atttaccaaa
     8221 gaagaacgga cttcaggtca atcatgctgg attttaggcg cttcgtatat tacagaaggt
     8281 gctataccgt ttgcggtatc tgacccgatc cgcgtgatac ctagtttgat gacaggttca
     8341 gcagtcgctg cagggatttc catggcagta ggagttactt caatggcacc tcatggaggg
     8401 atttgggtga tgctaattcc aaatgttatg aacaatttac ctatgtatgt cttggcaatt
     8461 gcatcaggta cagtcactac agcctttatg cttggactat tgaaaaaaac agtgaaagag
     8521 aaaaaacaag acaagtggac gaaaaatgaa aatctaacaa atgaggtgga agcgtaatgt
     8581 tatatacaat gaaagagtta ttgaaagtgg ctaaagagaa tcaatttgca gtaccagcct
     8641 tcaatatttg cagctatgat atgatgaaag caatcatcga agaagcagag agattagaag
     8701 caccagtaat cttagaaatt catcctgatg aaatcgccta tttaagtgat gagtttgttg
     8761 cggcagtaag agcctatgct tatacaagta aactgccgat cgttatccac ttggatcatg
     8821 gcggtacgat agaagatgtc ttacgggcga ttcgaaacgg ctattcttcc gtgatgatcg
     8881 atgcgtcttt gaaatcattc gacgacaatg tacagattac gaaaaaagtg gttgcgttag
     8941 ctcatgcggt aaatgtctca gtggaggctg agcttggaac gattgggaat aatggttctg
     9001 ctgaaggagg tgctgaaaca atcatttata cagatcctga tcaagcgaag atttttgtga
     9061 aagaaacagg aatcgataca cttgctgtag caattggaac agcgcatggc ttgtatccga
     9121 aaaacagaac tccaaaatta aatatcgact tactgaaaga attgaaccaa caaatcgatc
     9181 ttccatttgt tttacatggc ggttcaggta atccggacgc agaagtcagc gaagcagtaa
     9241 aatacggagt tgccaaagtg aatttgagct cagatcttaa aagcgtcttt ttcaatgctt
     9301 tgagagaaat attgagtgaa aatccatcga tgtacgagcc gaatgcaatt tatccagcag
     9361 ctaataaaaa ggtacaagaa gtggtcaaac ataaaatgga aattttgaac acagtaggca
     9421 aggctagttt atactaaaaa taaaaatgtg tggtcaagag ggtatgacaa tagtcttgtc
     9481 ataccctctt aaccacacac gatgttcaga agtagttttt tcgaaaaatt tcttcttatt
     9541 tctcgaagcc aatcacttct gtcacaacct cttttttcct gttactatat aaagtaaatg
     9601 gggtgatttt atgttgaatg aagtggagaa tagacagcag gagattgtga attatctaaa
     9661 aaacaaccaa tttgcaagag tcaccgattt gatcgagtta gtaaattata gcgaagccac
     9721 gattaaacgt gaccttgttt ttctggaaaa aagtggattg atccgtagag ttcgcggtgg
     9781 cgccatgctt attgacaatc gaaaaattga cgtgccttac ctgatgaaga tcaccaaatt
     9841 agaagaagaa acagaaaaac gtaagattgc agatacagct tcttctttga ttcgagatga
     9901 tatggtctta ttcttggatt ccagtacaac ctctttacac ttagtcagaa acctagcaag
     9961 atttgatggt ttgcagatca ttacgaatgg catcgtcaca gctgctatgt tgagtgaata
    10021 tacaacggca aaagtatctg tattgggagg ggcaattgtt ccgaaacgag caacaatcaa
    10081 cggttcaaaa gcctataacg atgcattgtc atattatgcg gatttagcga taatgagttg
    10141 tcgcgggcta gatttcagcc aaggagtaac agaaactcat gaaggagagg caatggtcaa
    10201 aaaagctttt agaaaacaat cagaccggct tgccgtttta gttacacagc aaaaaattgc
    10261 acataaattc atgcatcaag ccattgcttt tcatgaaatc gattatctgg tgacaagtaa
    10321 agaactgaca aaagaacagt atgcgaaagt aaaggaacac catatcgaat gtctgtacgg
    10381 ataaattatt acgtgaatgt tctaagtggc ttcctcattt ttagatagga ccttatcttt
    10441 gctcgcttct ctcagccgat ggtataataa tgaaaaggtt atcttgttag ataatcttac
    10501 ttttttatcc aggagaaagg agtgcagaaa atgaaacatg tgaaggcgtt ggtagtgaag
    10561 gcaatcatga tctgggcgat tctgtgggtc gtgctgacag gtttatacgg tgttagcttt
    10621 atggattcaa cgattgttgg ggtaattatt gtagtcatga tctatgtgct aggggatctg
    10681 ttgatcttac gtaaagtagg gaacatcgca gcgacgatcg cagatgctgg ttcagctgca
    10741 gttatcttgt ggctctatct atattttatg aatgatacag tcgatatttg gatgaaagta
    10801 ttgattcctg ctctattgat cggtgtggca gaatggttct tccacaagtg gttgctaagt
    10861 caaggcatcg taccagatga acggaaaatg gaataaatca acagttaaac tattcttatc
    10921 gataaatgaa agctttaaaa ataaatagat gtttaattaa gaataatcat aaactatttt
    10981 tactcaaaaa accgctcaga ttattttcag atctgaacgg ttttttttat ctattcatca
    11041 agatcaaatt tcatttttca atgattcaat caattcgccc tctgcttcat gactatgttt
    11101 tcggccatta acaagataga gtgctgtaca tatcaatgga atgactccgg caatcacata
    11161 caatcctcta aatgaagtaa gagattttag catccccatc aaataaggac caacgcctaa
    11221 gcctaggtct aaaccaataa agtaggtaga taaagcaata ctgattcgat gttcgcggac
    11281 gatttttaaa caaacagcct gtccatttga cataaacgta ccataaccta ggccgactaa
    11341 tgctccagag ataagcaatg tcacactatt cgtcgttaca ctcagtaaaa ataatcctgc
    11401 tgttaagaat aaatagctag gatacatcac gtattgttct cctttgacat cgaaaatcct
    11461 tccagtcaat ggacgtgtaa atgtgatgac caaagcatat acgacgaaga aaaatgaccc
    11521 agcagcaact aagtggatta cttttgtata agaagataaa aatgataaaa cacttgaata
    11581 agaaagaccc atcaaaaagg caattcctgt aatgaaaagt gctttctttt caataaaact
    11641 attgatattc catgagcgaa gagatatttt ttgttcttca gataatggaa tattttttac
    11701 aggaaagaca aggcaggcaa tcgtaacaaa gaagaccagt acaattgaaa accagataat
    11761 gaaacgaaaa tcagtcatat tcagcaaaat cattcctaaa aatggcccaa cagcggcagc
    11821 taaactagta cttaatccat aataattgat gccttcccct tttttagatg cagggatata
    11881 tgcagtaacg atagcatttg tagctgtaga aactgtaccg tagccgaatc cattcaagaa
    11941 tcgaactaaa tacaatgccc cgatagttgg cgtataaaga tatgctaatg ttgaaaggag
    12001 atagaagatc gcgccgcctc ttaaggtaaa cttccgacca aatagttcta actgtttccc
    12061 catgaataag cgtgccagca atgttccgat gatatagatc ccagaagcaa aacctgcttg
    12121 gctcaagctg gcgtgtaagg tgtcttgcgc aatgactgcc atgatgacca tcaacagata
    12181 ataaatcagg tagacaataa aattgatcaa tgtgattcca ataaaatatt tattaaataa
    12241 tttcttttcc atgtatgtta tacattcccc ttaagctgaa tatgctttta atttttgtat
    12301 ttgaacttct acatcgtgat aattggatgg atagcgatca acaagttggc gaataaataa
    12361 atctcttgta gacataaaca gatttaaaaa ttcttcgcat tcgtttgttg gattctcatc
    12421 cactgctttg atcaattcga tcgagttttg atacgcatca tttaaaaaga ctaaagaagc
    12481 tttcatatca aatcttgttt gatatgctgt ttctaatcga ttaatgattg tgttcatcca
    12541 gtaattcatg ttttttccct ctattcttaa aaaattttac ttctgcagta taacgaataa
    12601 aaaaaaggaa aaaaatcgca taagtgatgg aaaaaggtac ttaacaaatg ttaatatcat
    12661 tagtaaaata cctttggact ttaaaaagtt ttaaagtact gtatgatctt tttagatgaa
    12721 agtttggatg ggggagcatc attggatagt agagtcttaa aggaatattt ggataaaaat
    12781 gattttccga ttatacgcaa aggatatcat aaatatttga cattcgaagg gatggaagat
    12841 tcccatacgt atatcttgaa agagggaacg gtaaaagcaa gtgttatttc aaatgatggg
    12901 agagaattta accttcgcta tatcaaagat cttgaaatcg tctctctttt aaaagacgaa
    12961 tattcacaat tcatcgattc gccgtataat attcgagttg aatcagaata tgcagagttt
    13021 tatcgaatca atcgtgtgcg cttttggcaa aatatcaacc aagacccaaa gctacaacac
    13081 tatgtaaaag agtattaccg ctatcgtctg ctttattcga tgaaaaaaat gcagcaaatg
    13141 ctattaaacg gaaagctggg agctgtttgt acgcaacttt acgaattata tgatttattc
    13201 ggtgtgcgga cagaagaagg gatgctgatc gatttttcga tcacgaatga agaaatcgcc
    13261 aaattttgtg gcatctcaac tgctagcagt gtaaacagaa ttttaaaaca actgaaaaaa
    13321 gatggtatta tcaccgttaa agaccatcat atcgttattt tgaatatgga cttgttagaa
    13381 gacaatatta tcttttgatg aacaaagagg ttgtgaaatc agcgttttga cctgagtatt
    13441 agagaaacaa acggaaaaat agttcctcat attttgccgt tttttgagct aataccgagg
    13501 gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc tgccgtttaa ctctgagtca
    13561 caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac ttgtgaccga
    13621 ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc tgcatcaagt
    13681 aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg acttatcacc
    13741 gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga cacgattttt
    13801 gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc tgccgccacc
    13861 gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc ttccaggagg
    13921 tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat cggttttttc
    13981 agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg atttcaattg
    14041 ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca aaacaacagc
    14101 aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag tttcttcgtc
    14161 tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt tagacaggta
    14221 agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat catctaagat
    14281 cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg aaatgtagat
    14341 ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc cgactgcgtc
    14401 tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag tcgttacgat
    14461 caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca aagattggat
    14521 ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg catgagtagc
    14581 caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaattttt tcatcatatc
    14641 aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag agcggtcagt
    14701 cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg tccattcatt
    14761 gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag ccataccggc
    14821 agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac accggtcaat
    14881 gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt taattcagat
    14941 tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg gaacccggct
    15001 aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac gccttcgacc
    15061 atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa agaaagcttg
    15121 cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc tatccgccat
    15181 atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc tgtagcccaa
    15241 taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa agctttggga
    15301 acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc tttctcgaca
    15361 gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga tagtacgacg
    15421 ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac gatctctgca
    15481 ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga ttcgtccgct
    15541 gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt catatcggca
    15601 agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc tttgctttct
    15661 tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc ttcttctgta
    15721 tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa agtcaacgga
    15781 ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc tttctaaata
    15841 aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa tttagggatg
    15901 ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag cttaggctga
    15961 acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga attttctgtc
    16021 ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg attttgtttt
    16081 tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag ataagaacca
    16141 tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc ttcatttttt
    16201 ccggctgctt ccataccacg tgcaatggaa ccttgcacgt agccttcacg tacgctagga
    16261 tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc atcggtgtaa
    16321 gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg ccaagaatca
    16381 tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac ataagaaatc
    16441 cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag gaattcagct
    16501 aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg gattgcgttt
    16561 gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc ccgtaagaat
    16621 aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc gaatttgatt
    16681 ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt gacgccgcca
    16741 aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt gaacatggaa
    16801 ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc gatgattttt
    16861 ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt tccgtctgtt
    16921 aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg gactttgaca
    16981 ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc tagccattga
    17041 tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat aaaaaattcc
    17101 tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat aggaagatgt
    17161 gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt taaatacctg
    17221 aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa gagatagtga
    17281 attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt ctggcgttcg
    17341 cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa aaatgctatt
    17401 gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac cggaaagacg
    17461 agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga cggtgagcca
    17521 tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga tgtgatcgaa
    17581 atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga tcgggcaaat
    17641 tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca tatgctttca
    17701 acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag tgtcatattc
    17761 atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg gactcagcga
    17821 tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt tatcttgaaa
    17881 gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc tgcagaaggc
    17941 gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga aggaacaatt
    18001 actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat ggacagttac
    18061 ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa ccaaattctc
    18121 gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg ccgtgacttg
    18181 ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat cagaagtaca
    18241 tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat aaagattttg
    18301 agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt ggaagtcgct
    18361 acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt agcatttgca
    18421 gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa cgaaatcaag
    18481 gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca gcaaacaaat
    18541 cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa agttctgcgg
    18601 gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt gctgatgagt
    18661 ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc tggacctaca
    18721 ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa tgacgaagaa
    18781 ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc gcctgatcct
    18841 gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac gcaggcacaa
    18901 gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat cgagttgctt
    18961 cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga actgtttggc
    19021 tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta tgatgcgtgg
    19081 aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag aaatggcaga
    19141 agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg aattggtcaa
    19201 agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag aagtcgttga
    19261 tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg atgcgatcgc
    19321 acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt tgccaggatt
    19381 ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg cgaaactgat
    19441 cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac tcgcctttta
    19501 tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt taagtgtaaa
    19561 acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc cctgcgaaat
    19621 atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc cgaaagatgt
    19681 aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc atggtgtgtt
    19741 atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt tgcagcggct
    19801 gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag agggagaagc
    19861 aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga ctcgtctagc
    19921 acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt tattgaaagc
    19981 tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga tacaatataa
    20041 aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa tcgaggggcc
    20101 tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga aagaagtagc
    20161 aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg aagagatccg
    20221 agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag cactgcttta
    20281 tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg caaaagggaa
    20341 aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg caggccgacg
    20401 catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta ctacgccgga
    20461 ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca agaaaaacag
    20521 acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc gtgtacgtca
    20581 tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg atgcaagaaa
    20641 aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt atccgcaatt
    20701 ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat ccaagacaaa
    20761 gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac aaaactatca
    20821 tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat tgaagatgac
    20881 cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa gcaatttgta
    20941 tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc agtagaagta
    21001 gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca attttaggag
    21061 gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg tgtatcagca
    21121 gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg aaggggagaa
    21181 aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct gtacccaaat
    21241 gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa agaaagaaca
    21301 tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc agatcagacg
    21361 tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt ttttgatcaa
    21421 agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct tggaagagcc
    21481 ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc tccctactat
    21541 tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac tgatccatcg
    21601 attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct taacaaacag
    21661 tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta aggacttgat
    21721 ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct atgtccaaaa
    21781 aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat ctgtcttgat
    21841 gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg gcaaagtgaa
    21901 acagataaac cagataattg aacggatatt gatcgcagag cagaaactac gcagcaatgt
    21961 cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc ttgccgcaaa
    22021 ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata tagagatgca
    22081 ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga caaggtatta
    22141 gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa agagattgat
    22201 cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca agctgaatta
    22261 gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc aaaagaaaaa
    22321 gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg aatacacatt tgatcgaaat
    22381 aaattgattt tttctttcac agcggacggg cgaattgact ttagagaatt ggtaaaagat
    22441 ctggccgctg tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg cgatgaagca
    22501 aagataatag gcggaattgg tccatgtggg cgtccattat gctgttcttc ttttttaggc
    22561 gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa gtttatcttt gaatccaaca
    22621 aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc aatatgagag cgaagaatac
    22681 gagacagcga aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc tgatggtaaa
    22741 gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta gtattcggct gactggacgg
    22801 gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat ttatccaagt aggtgagatg
    22861 aatggataag agatcattat acgacggatt gaatcaatta gaatcagagt tgagaaatac
    22921 attaacagaa cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa aaaatacaac
    22981 tttggagatg gaaaatcaac gtctgcggga acacctgcgg gaattgaacc aagttgcaca
    23041 agctccagct gaaaatgtga agcaggaact ttctaagtct cgtatgaatc ttgaaaaaat
    23101 ttatgaagaa ggattccatg tctgctacga tttatatgga tcaagaaggg acaatgacga
    23161 gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac caataacaag
    23221 aggagactgg agcggagaaa tactgctcca gtctttttat taaggagatt gtgacagatg
    23281 tataatcaaa aaagtttcaa gggaaatccg gtatatgggg atttatatct tgtaccgacg
    23341 ccaattggaa atttagaaga tatgagctac agaagtgttc gtatgctgca agaatccgat
    23401 cttatcgcaa gtgaggatac gcgtaataca caaaagttat tgaatcattt tgaaatccat
    23461 acaccccaaa aaagtttaca tgaacataat tataaagaac gtgtccctca attgattgct
    23521 ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc ttccatcagc
    23581 gatcctggac atgagctagt tttagcctgc ataaaagaag ggatttctgt gatctctatc
    23641 ccaggaccta cagctgggat gacggctttg attgcttcag ggctagtgcc tcaaccattt
    23701 ttattttatg gcttccttcc acgtaagaaa aaagaacaga aagaaacatt gaaaaagcta
    23761 aaagaacaga cagttacgct gattttttat gagtcacctt atcggatcac tgctacattg
    23821 acgaatttct tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga attgacaaag
    23881 gtccatgaag aataccttag aggaagtacg gaagagctac tcgcttatat cgaagaacat
    23941 ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat gccagtgacg
    24001 gaagttgatg aagagttggg aacgttaaag gaacaagtag aagcaaagat cgcttcaggc
    24061 gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa atgggctgaa aaaacaagaa
    24121 gtctaccgtc agtaccatga attagactga gatagtataa gttgtctata atgaaagact
    24181 aatgaggaaa tcagctacta acttttcaaa tctcttgaac gcgaacgaat gttcttgtat
    24241 aatgaataag aggtgaggat gatggagctg caatttcctt tacataatga tacatcaaga
    24301 aaaatcattc atattgatat ggatgctttt ttcgcttcgg ttgaagaacg agatcatcct
    24361 gagtttcgcg gacatccatt agtaattgcc agacacccca gtgacactgg tgggcgtgga
    24421 gtagtgacta cagcaaatta tgaagcaaga aaattcggaa ttcattcagc aatgagtgca
    24481 caaaaagcgt atgagctttg tccgcaagcc atctttaagc ctggcgacca tcataaatac
    24541 agcgatattt ccaaacaagt acgggaagta ttctatcgtt atacagatat tgtggaacca
    24601 gttagtatcg atgaagcgta tcttgatgtg accgaaaata aaatcaattg caagtcagcc
    24661 atcaaaatcg cccgaatgat ccaagcagat atttgggaga gtgtccagct aacatgttct
    24721 gcgggagtaa gttacaataa gtttttggcc aaattagcat cagactatca aaaacctaga
    24781 ggaatcacgg tagttgcccc tcaagacgct gtgccatttt taaaagcttt gccaattgaa
    24841 aaatttcatg gggtagggaa gaaaactgta cctagaatgc atgagctagg gatttataca
    24901 ggaaaagacc tatatgaatg cacagaaatg atgttgattc gaaattttgg aaaaatgggc
    24961 tattctcttt atcgtaaagt cagaggaatc catgattctc cagtaaacgt tacaagagag
    25021 cgaaaatctg tgggaaaaga gcatacttat gggcagccgt tgcaaacgga agaagcagtg
    25081 ctgactcagt tgcgccaact agctgaaaaa gtagaagaag cactgagaag agtccaaaaa
    25141 catgggaaaa cagtagtttt gaaggtcagg tacacggact attctactgt gaccaaaaga
    25201 gtgacattgc cagaatatat ctataaaaaa gaagcactct tttatcaagc aagccttata
    25261 tgggaggaaa tcttgggagt agaaaaagga atccgattgt tagggattac tttgacgaat
    25321 ctggacccga tgacttatga aaacatcgtc cttccattat gggaaaatca agaaatataa
    25381 aaacagcttt gagagttgaa accggaaatc cgttttctct cccaaagctg tttttatatc
    25441 aagtcttgct ttttgatttg ttcataggtt tctgttgcat ccgtacattt ttcccaaatc
    25501 acaacttgat tggattctaa agatttttgg acgtcaatga tccgctggtt gctacttccg
    25561 cgaaattgta gttttaggtc acgtttgctt aattcaaagc gtccgtcaac aaggatatcg
    25621 atttgcgaaa ggagttctaa tttatcttct gaatcttgta gcaattcatc aaaggtatag
    25681 cctgtccatg accagatatc ttttgtcgta ccgaactctt ttcggatacg tcgtacgact
    25741 tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa tccttgcacg
    25801 taatcgttgc gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa tggtttgcca
    25861 tatcgaaaat tttgtgcagc tttgttgaag cagccttcgc aggcaaataa gcagccacta
    25921 acgtaaagac tgttgcggac accttcacca tcgacaaaat taaacggttt gtagtcagca
    25981 atatagtttt ggctgtagtc ttttgctaac cattcttttg gttttggatt tctcataaaa
    26041 aaacttcttt catttaaggt tcaaagatat agctattgaa gataaaatct actcgaccac
    26101 ataaataaga acaaactgac ctaaatccat tgttcaagaa agcttgttct tattttgata
    26161 gagggcgttc aaagatacaa agccatcgaa agagggtggt gaaaaaagac ttgtcatagc
    26221 ctcttattct gaataaacgg tgttcaaaag cttgcatctg cagtaataag cccaactaaa
    26281 cgaaaaatat gaggaactat ttttgtttag ctattcttat tacttgatgc aggacagctt
    26341 tttcacaacc tcatttcatg tgtttcacac gtgaggaaat ttctttatgg cggccatgta
    26401 ccatcggacg agcttgcgga tttcctaaat agccacaagt acgtttgact acatcacatg
    26461 tttttggatc atgatttcca cattctggac attcgaaccc tcgttctgtc ggtttgaaat
    26521 caccttcgaa accacattga tagcaatgat cgattggcgt attcgtacca agatacccga
    26581 ttttgtcata tgcaaaatcc catacagctt ctaaagcttt cggattttgt tggagcatag
    26641 ggtattcaca gtagtggatg aatccacctg aacaatattt tggataatcc ttctcaaatt
    26701 ccaatttttc aaatggcgtt gggtttttac gcacatcata gtggaaactg tttgtataat
    26761 attctttatc tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa cggcagaaac
    26821 ggtcagttag actttcactt ggagttgaat agacactgaa atgataacca tattcatccc
    26881 cccatgcatc tgcattagct tttaattctt tgacgatatc aagtgtgaaa gcttttgctt
    26941 ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc aacttcatac aatccaatgt
    27001 agcctaacga tacagtagct cgacggtttt tgaataattc attgacagaa tcggtacgtg
    27061 aaagacgttt cccgaaagca ccatacatat ataaaattgg cgcatttgct ggaatggctt
    27121 ctttacaacg ttctacacga tagagaagcg catctttcat gatttctaag cggtcagaca
    27181 gtaactgcca gaatttattc tgatctcctt ttgattcgat cgcgattctt ggcaaattca
    27241 atgtaacaac acctaaattc attcgtccga catttacttc ttgaccattt tcgtctttcc
    27301 acccttgcaa gaatgaacga cagcccatag gtactttgaa acttccagtt aattcaacga
    27361 ttttatcgta attcagaata tcaggataca ttcttttcgt tgcacattcc aatgctagtt
    27421 gtttgatgtc gtaattagga tcttcagggt ttaagttcac accttttttc aaagtgaaga
    27481 tcagtttagg gaagatagct gtccgctttt cactccctaa tccattgatt cgaatctgta
    27541 aaattgcttt ttgaatttct ctttcaaacc agttttctcc tagtccaaat cctaatgaag
    27601 tgaaaggagt ttgtccattt gaagtaaata aggtattgat ttcatattcc aaactttgca
    27661 tagcatcgaa gatatctttt ttggtttttg cttttgcata ttctttttgg cgttctggcg
    27721 aatcgatcca ttcttccgcg tcttttagat gttttttata gttcaattct gcaaaaggag
    27781 caagcaattc atctgttcgg tcggcagaac agccaccata ttgacttgac gctacattgg
    27841 caataatctg tgagatttgt gctgtggcgg tctggataga ttttggcgat tcgacttcgg
    27901 cattgccgat tttgaaccca ttgttcagca tgcctttgaa atcaatcaag cagcaattcg
    27961 tcatcggtgt ataaggatga taatccaaat catggtaatg gatatcgcct ttttgatgcg
    28021 catttgctat atgtggtggc aacattttca gtccgattga ttttccaacg atcccggctg
    28081 tcagatctcg ctgtgtattg aaaacattac tgtctttatt tgcgttttcg ttcacgacag
    28141 tttggtcctt gttgattagc ttttcaatcg aaaagttgat atctgttgct ttactgcgag
    28201 caaaatctcg tttggtacga taattgatgt actcttctgc taattcatat tcgttttttt
    28261 ctagtaatat atgttccacg atgttttgaa tctcatagat tttgacattt tcagcaaacc
    28321 ggcttttgat ttcttggaca accaagtctg caatcatttg aattttttca taagtaagtg
    28381 gtgataatga gccgtgaatt ttcttttctg ctttgatcag tgcttcattg atcttttgtt
    28441 catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt catctgggat gtctctggct
    28501 gctgagtaac gacagttttt tcttcataat ttactttcat tatcttcaac tcctgctaaa
    28561 ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca actaaatatc ggctatatat
    28621 agtattcaat gtgctgtata a
//
LOCUS       DASNNU010000030        27568 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.030, whole genome shotgun sequence.
ACCESSION   DASNNU010000030 DASNNU010000000
VERSION     DASNNU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.030"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(70..969)
                     /locus_tag="U8116_001219"
     CDS             complement(70..969)
                     /locus_tag="U8116_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8434227.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(1134..1958)
                     /locus_tag="U8116_001220"
     CDS             complement(1134..1958)
                     /locus_tag="U8116_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN8434228.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(1969..3264)
                     /locus_tag="U8116_001221"
     CDS             complement(1969..3264)
                     /locus_tag="U8116_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEN8434229.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(3280..3759)
                     /locus_tag="U8116_001222"
     CDS             complement(3280..3759)
                     /locus_tag="U8116_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEN8434230.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(3765..4793)
                     /locus_tag="U8116_001223"
     CDS             complement(3765..4793)
                     /locus_tag="U8116_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEN8434231.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(4810..5916)
                     /locus_tag="U8116_001224"
     CDS             complement(4810..5916)
                     /locus_tag="U8116_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN8434232.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(6145..8742)
                     /gene="adhE"
                     /locus_tag="U8116_001225"
                     /gene_synonym="adhC"
     CDS             complement(6145..8742)
                     /gene="adhE"
                     /locus_tag="U8116_001225"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEN8434233.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(9047..9427)
                     /gene="yajC"
                     /locus_tag="U8116_001226"
     CDS             complement(9047..9427)
                     /gene="yajC"
                     /locus_tag="U8116_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEN8434234.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(9524..10669)
                     /gene="tgt"
                     /locus_tag="U8116_001227"
     CDS             complement(9524..10669)
                     /gene="tgt"
                     /locus_tag="U8116_001227"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEN8434235.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            10887..11585
                     /locus_tag="U8116_001228"
     CDS             10887..11585
                     /locus_tag="U8116_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN8434236.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(11753..14392)
                     /locus_tag="U8116_001229"
     CDS             complement(11753..14392)
                     /locus_tag="U8116_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN8434237.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(15011..16042)
                     /gene="queA"
                     /locus_tag="U8116_001230"
     CDS             complement(15011..16042)
                     /gene="queA"
                     /locus_tag="U8116_001230"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEN8434238.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            16235..17409
                     /locus_tag="U8116_001231"
                     /pseudo
     CDS             16235..17409
                     /locus_tag="U8116_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            17413..18054
                     /locus_tag="U8116_001232"
     CDS             17413..18054
                     /locus_tag="U8116_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8434239.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            18051..18968
                     /locus_tag="U8116_001233"
     CDS             18051..18968
                     /locus_tag="U8116_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN8434240.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            18965..19636
                     /locus_tag="U8116_001234"
     CDS             18965..19636
                     /locus_tag="U8116_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8434241.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(20177..20665)
                     /locus_tag="U8116_001235"
     CDS             complement(20177..20665)
                     /locus_tag="U8116_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEN8434242.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(20866..21132)
                     /locus_tag="U8116_001236"
     CDS             complement(20866..21132)
                     /locus_tag="U8116_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN8434243.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(21132..21395)
                     /locus_tag="U8116_001237"
     CDS             complement(21132..21395)
                     /locus_tag="U8116_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN8434244.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            21871..22056
                     /locus_tag="U8116_001238"
     CDS             21871..22056
                     /locus_tag="U8116_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN8434245.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(22198..24021)
                     /locus_tag="U8116_001239"
     CDS             complement(22198..24021)
                     /locus_tag="U8116_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEN8434246.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(24619..27324)
                     /locus_tag="U8116_001240"
     CDS             complement(24619..27324)
                     /locus_tag="U8116_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEN8434247.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         7870 a         5914 c         5031 g         8753 t
ORIGIN      
        1 aaaataaccg cagtttctgc ttctatgtaa acaacagcag ctactgcggt tattttttag
       61 tgatcattct cattgttcat cttcaaaatg ttcataatga accaactgtt tctgtgtatc
      121 acgtttcaaa aattctttag gcgttatgcc gaactctctc ttgaatgctt tcagaaaatg
      181 cggataagaa gaaaaaccac ttttactata tacagcacta gagctgcggt attctcgcaa
      241 caagtgcttg gcatagatca gctttttctt cattacatac tgatgaaacg taaatccagt
      301 gtacttttta aattctcgtg tgatatggta tttgctgaca aaaaactgct tttccatttc
      361 ctctaaagaa agcggatgag ataaattttt tgagatatat tgtaacattt gttccacacg
      421 ctcattagga actacagcat tctgttcttg cgcactctct tctttttgta caacttgatt
      481 gagaaaaatc aagtattcta ccagagcttg ttcatatttt atgtcttcac cgtaaatttt
      541 tttatcagga ggatggtcga taaatgataa tttttgcttc aatacttcct gttctatttg
      601 caatacacga ctcctcttct ctcccattgg ttgaaaacaa gccgatagat tagaccatgg
      661 cgtggaacga ctttctagaa aagaaggggt gacaaagata tagacacgct cgaatacatc
      721 tgaagattgg cggacgatac gatgcaaatc attggaatgg atgagcagca cacttcctgc
      781 tttgatcgta aattgtcgtc cgttcaaata aaaagtcgct tctcccttta atgtagcatg
      841 gatctcatag aaatcatgat gatgatagat cgttttattt tggctgacga catctttgac
      901 atgatagatt tcatactgat cagaaaccat cgtattgact tgttcaccgc tgattttctt
      961 ttggagcatg tgccgcacct ccttttgttc acttatgcac aattttatta taccacgttt
     1021 tttgtaatca aaaagagctg cttttatata ctcttataaa aaagaagaga cgtggcatct
     1081 tctagccccg ccccctccat tctaaatatt agttaaccaa cgtcttcttt acattattca
     1141 agaagacttc ttccataacg aatcccatca tcttttgttt cttcaagaac cacatacagg
     1201 tacggacgat tcttcatgat gatttctttg atttttgtgt actcgatcac accgtctccc
     1261 aaatttaccg gcacgatttt ttcctgttcg acacgaaaat ctttcaaatg aaccgcacat
     1321 atcttttcac caaaacgttc aaaagcttct tctaccaatt ggacttgttc ttgataatca
     1381 ctacttgtga tcaaattggt cggatctaaa atgattccta gatagtccga atctactgct
     1441 tggatcagct gctctaccct tgctaaagaa tacaccggat gattcaaacc tggttctatc
     1501 cccaccatca tttggtattt ttctccagct tttaccaagc gacggataac ggacacagct
     1561 tcagcaaatg cttcatctgt gaaattttct tccgtatatt ggatttccgg caaaacacat
     1621 ccagtttccg aagcaaccat cgacgcacca aaggaagaag catagcgaag atactgctcg
     1681 aactttgtca gcaactcttg tcgtatcgtt aaatctggat gaatcatatt gatatagcaa
     1741 cttaaaattc ctacggtcac ttcttgcttt tctaaaactc gtttgacata atttcccatc
     1801 ccagaattga tctcatttgc gccagaaggt atttcaggaa acgaaagacc taaggcgagc
     1861 tgcagtgtat gaatcccttg ttcttttgtt ttttctgcaa gatcttctat cgtttggact
     1921 tcggaaagat catgtcctct aattgtaatg ttcaacataa ctattctcct aactcagcat
     1981 attgggtagc cacatgacga tttgcgggaa gtaagtaatc aaaaacaaga cgactaagat
     2041 aaccccataa aaaggaagaa gcggtttcag cattgcctct gcttttacgc ctcctacact
     2101 tgcaccgaca tatagtcctg tacctactgg cggcgtgatg gagcctacac acagattcat
     2161 gattgaaaac aacccgtaat gtacaggatc aacccctaca cttgtgacga ttggtaaaaa
     2221 tattggtgta aaaatcaaga tagcaggcgc cacatccata aacataccca ccagtaataa
     2281 cacaagattg actaataaca gaataataaa tttattatcc gttacgccca taatcaacga
     2341 actaattgcc tgaggaatac cggtaaacgc catggcgaaa gacatcatgg aagaagtagc
     2401 caataagaac atgatcgtcc ctgacattgc tactgattga aacaacatat taggtatatc
     2461 ttttagtttt accgttttat agtaaaacat agcaatcaac agggagtaaa tgacagctat
     2521 cgctgaagct tctactgctg taaatatccc cgtcaagata ccaccgataa tgatgacaat
     2581 caatagaagg ctaggaatcg cctcttttat tatttttcca gcatgagcca tctgtccttt
     2641 ttcgacaaca gggtatttat tctttttagc aacgatgaat gctactagca tgattcctag
     2701 cgcccacaaa gcgcctacga gataacctcc cataaacaaa gcagagatcg aagcaccacc
     2761 agcaacaagt gaatacatga taaaagcagt acttggagga atgaccatac ctgtcggagc
     2821 tgacgcgata ttgaccgctg tcgcaaactt ccggtcatat ccttcttcca cttgctgagg
     2881 gatcaacact ccgccaatcg cggtagaagc ggcaatggcc gagctagaga tggaaccaaa
     2941 taaagcattc cctaacacat tcgtatgagc caaagccccc ggcacgcgcc cgacgaacaa
     3001 catcgcaaaa tccactagtt tcttagctat ccctccatta ttcatgataa tgcccgagag
     3061 aacgaaaaac ggaatagcga tcagcgaaaa actattgata cttcccacca ttttttgagc
     3121 tgaactaaaa gcagaaacat caaacggtat aactaacaat aaagtcatca tcgatgagaa
     3181 tgcaatactt atagcaatcg gcatccctaa tacaagcaac acaacaaata caaaaaataa
     3241 cactaatccg gcttctattg ccattttatt cctccatttt tatatttttt cttgaattgc
     3301 tggcttgttg cttaatgtat cgattagatt gatcaggcta tagataatga tgagtacccc
     3361 cgatacaggt actgacagat aaacatatcc cataggaata cttaaagaag gcgagatctg
     3421 ccccattgtc aatgtgacag cattccatcc gccatacacc atgatcatcg cagcaaatac
     3481 cagaaataaa ctttgaacaa caacttctaa aattttactt tttttgccag ttaatttttc
     3541 acttaaaacc gttacggcta aatgtgcttt ttgtcccaca acatacgcgg aagacaacat
     3601 ggcaaaccag acaagaccga atcgaacaag ctcttccgtg accgtatttg gatttcctaa
     3661 aaccgttctg gaaaatactt gccataaaac aacagcgacc atcccaacaa gcaaaacagc
     3721 tcccaggatc tctaatacct tgtttaaaat cattttcatc acagctacgc tccttgctct
     3781 tggatctttt gataaaggtc tttgtaggta ggatcgtttt tgaatatatc atgaagtggg
     3841 gcaactgcct ctcgaaacgg tgtattatct acatcattga attggactcc atgttcttct
     3901 tgtgctaaag ctttttcttc ttcaattgct tttgcaaaag cttcaatttc aaaaaccgtt
     3961 gattctttcg ctgcttcttc aatggctttt tcctgctcat cagttagctg ttctttgatg
     4021 gcatcattca tgatgatgat atctggcaca cgtgtatgct catcatagga atagtattta
     4081 gccacaccac catgtccagc tgtatacaag acaaattcat tgttttctga tccgttgatc
     4141 aaattggatt gaagagacgt gtacacttca tcacttccca taggtacggg cgaaccgcct
     4201 aaaagctcta ccattttgat agcggtttcg ctttgcatca cacggatctt ctttcccttc
     4261 aaatcttccg gcgtatggat cggaccgtct accatataaa agctgcgctg accagaatca
     4321 taataggtca atccggtaaa tcctaaattt ttggtcgatt ggtagattgg ctccattatt
     4381 tcttcattat ccatcacctt aaagaaatgt tcttcatcat tgaatagata aggaactgaa
     4441 aaaatcgaat aatctttaga gaaactttcc aacgctgagg cacttacttt tgtgaaatct
     4501 actgctcctg actgtgtcaa ttcgatcagt tcacgttctc cacccagttg tccatctggg
     4561 aaatactgga tctgtacgga accatctgtt ttttctttta ctaaacgccc aaattcattc
     4621 attgcatcaa ttactggctg gctgttgctt gcataagcaa agcgcaaggt aactatctca
     4681 tttacatctg catctgcgac agttgccaca ctagaattat ttccgcagcc gcttagacct
     4741 agcagaccta ggctaagcga acttgctgca aacagcagtg atgttttttt catatgggtt
     4801 tcctttcttt taacaataag agacgagcaa ttctgtcaaa caaagcaccg ttaacgactg
     4861 tccataaggc attgctgtga ttccgatatt ccgataaaaa tctaaactgt cccccattcc
     4921 tgtcccgatt gaaacgtttt gaacttctcc tttttcatcg atttgttcaa gcaatccttg
     4981 gatagcacga taagcgactt ctttatattc ttgtggtaaa taacgtttat ggaccgcttt
     5041 caaaataccg taagcaaatc ctgcagttgc agaagattct acgtaagaag aaggatcatc
     5101 caacaacgta tgccataaac cgctttcaga ctgataagtt tttaatgcac gcacttgtgc
     5161 ttcaagtgtt tcgatcaata atttgcgcaa tgcatcttct ttcgataatt ctaagatttc
     5221 aatgatctct ggaatagcaa tcgtcaacca gcagtttcca cgagcccaaa atgcttcggc
     5281 atagttatga tttccttcaa acgtccagcc gtgataccac aatcctgttc gtttatccat
     5341 caagtatttg atatggatca taaactggta acgagcttct tctaaatatt ctgggcgatt
     5401 caacagtttc ccgatttttg ccaaaggaag cactgtcatc atcaatgtat cgtcccacaa
     5461 ctgattctta ttttccggtc catatgtggc atgctgtaac ccgtcttcat ttgtccgagg
     5521 catgtcattc atgacccatt ctgcccattg ttctaaataa ggcaaatagc gtgaatcttt
     5581 cgtgtcttca tacaagtaag ccatagtcaa tagcggtgcc attgtattga cgtttttggg
     5641 cggagcgcct tcttccatac gatcctcaaa ccattcattg accacttgat aagcgcgagg
     5701 attttttgtc atctggtaat ttttataaat accatataga ccgacacctt gtggccaatt
     5761 ccacactgtc cagcttttgt catctacttt cagtccatca aagtccaaca aaaattcacc
     5821 attatcgtct ttgatcgttg tcaggttttc aacgagcagg tcaatttttt ctttcactaa
     5881 ttctttaggt acaaaaatct cttctttaat aatcatcttt acgccacctt tttaaacgct
     5941 ttcattttat atccttaata tagcagtaat accgtccact ataaatatcg ctttttgaac
     6001 aaaaactgtc taaaattgac ctaaatgtta aataacaaaa gtaactttca gttacttgga
     6061 aattaaaaaa agaacttgga gaaacaacgt tttccaagtt ctttttcatc tattttctta
     6121 tcttttttct acgctttctt ttgcttatcc gttgtaagca tcaatgatga tctgtttcaa
     6181 ttcagagatc aatggttctt tcggattagc tgtcgtacat tggtcttcgt aagctaattc
     6241 agccatacgg tctacggttg tatctagtgt ttcttgtgtg actccttgag cttttagact
     6301 catgttgatg ccgacagatt ttcctaattc ataaacagcc gttgctaaag cttcgactaa
     6361 ttcctctgtt gtttcgcctt tcagacctaa gaattttgcg atatctgcgt aatctgtatc
     6421 tgcacggaaa tagtcatatt tagggaacat cgcatgtttt tgtggatctt ttgcattata
     6481 gcggataata tgcggtaaaa gaatcgcatt tgtccgtcca tgagggatcc cgtattcccc
     6541 gccgatttta tgtgcgactg agtgacaaat acctaggaat gcgttagcaa acgccatacc
     6601 agccattgta gaagcgttgt gcattttttc acgtgattcc atatcaggtg tttttactga
     6661 tttttctaaa tagtcaaaga ctaacttgat tgcttgtagg ctcaatccac gtgtataatc
     6721 tgaagccatg actgaaacat aggattcgat tgcatgtgtt aacacatcca tcccggtatc
     6781 tgctgtgaca gatgctggaa ctgacatgac gaattgagga tcgacgattg cgacatctgg
     6841 cgtcaatgca taatctgcta acggatattt cacatgtgtt tcactgtcag tgatgaccgc
     6901 aaatggcgtt acttcagaac cagtccctga tgttgtcggg atacatacga attgtgtttt
     6961 ttctggtttg tcgatcttat atgtccgttt acggatatcc aagaattttt gttttgcacc
     7021 aaagaaagat gtgtctgggt gttcatagaa catccacatt cccttagctg cgtccattgc
     7081 agaaccgcct ccaagagcga ttactgtatc tggcttgaag tctgccatca gtttcgttcc
     7141 tgcgtatacg gtatttgtcg atgggttcgg ttcaacatct gagaagactt cgatcttcac
     7201 atcattttta cgtcgtgcca atacttcacg aactgtatcg cagtaaccga attggaccat
     7261 accagggtca caaacgatca tgacacgttc aacgttttcc attttctcta agtacaatag
     7321 agagttcttt tcaaagaaga tttttggcgg taatttgaac cattgcatgt tatttctccg
     7381 tttcgctaca gttttgacat tgattaagtt tacagcagag acgttacgag aaacagagtt
     7441 cttaccataa gaaccgcatc ccaaagtcaa tgacggaatc atttcgttat agatatcacc
     7501 gatgccgcct tctgcagatg gtgtattcac taaaatacga caagctttca ttcgaagacc
     7561 gaatttgaca tgcaactctt cgttttctga atggataact gctgtatgtc ctaatcctcc
     7621 aagattcaac ataccttcac acaaatcaaa cgcatggtcc gtgttgttag ctttgatcat
     7681 ggcaagaact ggagaaagtt tttctcttga tagcggataa tctgggccta cgccttccaa
     7741 ttcagcaatc agcattttcg ttccttttgg tacactgatt cctgccaatt ttgcgatttc
     7801 catcgcagaa tgtccaacga ttgatggatt gaccgcatat tttccttcgt tcatcactgc
     7861 atcttctaat ttttgcaatt catctggttt tacgatataa acttggtgtg cttggaattc
     7921 tgcttttact gctgcataga tttctttatc cacgatcaca gcttgttcag aagcacaaat
     7981 cattccatta tcaaatgttt tcgaaacgat caaatcgttc actgcacgtt taatttttgc
     8041 tgttttttca atataagctg gtacgttacc tggacctaca cctaaagctg gttttcctgt
     8101 agagtaagca gacttgacca ttccagctcc acctgtcgct aagacgatcg cgatacctgg
     8161 atggttcatt agcatagaag tcgcttcgat cgaaggatgt tcgatccatt ggatacaatt
     8221 ttcaggagct ccggcagcga tagcagcatc acgtaccact cgtgctgctt ctgcagaaga
     8281 tttttgggca cttggatgga aagcaaaaac gattggattt cttgttttca acgcgatcat
     8341 tgatttgaaa atcgttgtgg aagtcggatt cgttgttgga gttactccgc aaaccacacc
     8401 gactggttca gcaatttcaa tcaacccttt ttggacatct tcattgatca caccaactgt
     8461 tttatcatgt ttgatattat gccaaatata ttcggacgca tacatatttt tgattgcttt
     8521 atcttcgtaa acaccgcggc ctgtttcctc tacagccatc ttcgctaatg gcatatgttg
     8581 atccaaagct gccattgcca tttcatgcac gatatggtct actttttctt gatcaaagct
     8641 ttccatttct ttcaatgctg catttgcttt ttctgccaaa tcatcaatca ttgcttgtac
     8701 gtcaattgtc ttcgtttttt ctttttctat tactttagcc atcattatcc tcctaatggg
     8761 tcatggttat ttgttaaata attcacatgt atatcataca atatttattt ttctatgtaa
     8821 acactttttt gtgatttttt tcacagttat ttttaaatat aaaattgctt tatttacagg
     8881 tgtttgtata gaaaaatata taaagttatt tattattatt tatatgacac acccgattat
     8941 tcaatattga gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg
     9001 taggacatct ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct
     9061 tctcttctgt ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg
     9121 gttcttctgt tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga
     9181 ccgttttgat tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg
     9241 tccgtttgtc attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg
     9301 aatcacctgg tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg
     9361 aacgtgacat aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg
     9421 ttcccatttt tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg
     9481 ctgataacac tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt
     9541 tttctttatt gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat
     9601 ccatgattgc ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag
     9661 aagtcaaacg aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg
     9721 tatagttttt acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat
     9781 atttcgcatt tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga
     9841 ttcgagtagg caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg
     9901 aatcagcagc accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag
     9961 tgaattctaa tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg
    10021 agtaaccagg gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa
    10081 accctgctcc ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca
    10141 atcctcgttc tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt
    10201 catcgaatgg tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga
    10261 tgttgattgc tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat
    10321 gcacgccttc ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg
    10381 aatctgttaa aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta
    10441 ctaagtcttc accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac
    10501 ccatttcttt caactcttct ggggacattg tttttactgt agctaatgtt cctactggca
    10561 tgaacattgg tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag
    10621 tatgtttttc tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta
    10681 ctttctatat aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc
    10741 aagttccctc ggcttccttc tactttattt aatattcttt tttttatcct ttttttatga
    10801 ataacaaaaa aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg
    10861 ttattaaaga aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa
    10921 aagagagtct aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga
    10981 ttggtattgc tactattctt cttattatga ttcctattat cttgtttgcc accaccatat
    11041 atgatggatc agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg
    11101 gtggcggaat tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt
    11161 atcttgatat tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg
    11221 gattcaaagg tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta
    11281 caacattatg gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc
    11341 aaagctattt tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg
    11401 atacgatcac tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag
    11461 atctttcatt tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat
    11521 ggctcagccc atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata
    11581 tttaaaagag agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag
    11641 gcagtaaaag aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca
    11701 cctccttttt gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct
    11761 cttcttctgc ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga
    11821 tagataaaat ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt
    11881 cttgcgtaaa tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt
    11941 ctaccaaagc tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat
    12001 tgaacacatg cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat
    12061 gctgacttgc gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa
    12121 acaaggttac gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag
    12181 caaagatccc ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct
    12241 ctcttcctaa agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca
    12301 cagctgtaaa tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg
    12361 aaatattaca gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc
    12421 cttgactgac agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg
    12481 cactttgcgc cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca
    12541 acgctggtgc atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc
    12601 aagatttgac gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt
    12661 tgacacgcgc atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt
    12721 ctgacggatc attcaagata cctaattctt tcgctatggc actggcagtc accacatgat
    12781 ctccagtgat catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac
    12841 tttctggacg tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca
    12901 gtgcttcaga agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca
    12961 ctctcagtgc ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg
    13021 aactaaagcg cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc
    13081 ccatttggtg aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc
    13141 gtggatattt ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga
    13201 tggctacttc tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca
    13261 catctgtaca tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt
    13321 cgatattcgt tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg
    13381 tcagcgttcc tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg
    13441 gcaactgtcg gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca
    13501 acgtaacaat aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca
    13561 tgaacatctc aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag
    13621 ccgctaaagc gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg
    13681 ttttttgcat agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc
    13741 caatggcagt gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca
    13801 tattaagttg gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa
    13861 ctgcttcact ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca
    13921 aacgggcatc ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca
    13981 gttgctctga ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat
    14041 tcatatcttg caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata
    14101 ccgagttgat cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg
    14161 tagcaaatgc ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat
    14221 cggacaagct tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat
    14281 tcaatccctg cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat
    14341 ctgcttctgt taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc
    14401 tcctttaaaa tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt
    14461 tacttcaaat aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta
    14521 tatttgcaaa cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca
    14581 aatagacaga agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag
    14641 gtttcaccca gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt
    14701 tggatcacga agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa
    14761 cgtctgaaaa ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa
    14821 aatttttgtt taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac
    14881 aatcatttct gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct
    14941 cggctttttc tttgtcataa aatctttgta aagctttttt gttattcaaa actagcggct
    15001 tttcataacc ttatttcacg aacatcgcat ctccaaaact aaagaaacga tagcgctctt
    15061 caatcgcatg ctggtaagca gataaagtca attctcttcc tgcaaaggca ctaactaaca
    15121 tgactaaagt cgattttggc aaatggaaat tagttgagaa agcttctacc actttgaatt
    15181 catagccagg cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt tctccattaa
    15241 atttcgttcc aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc acgattttcc
    15301 cgccattttt acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt
    15361 cactatgcat atggtgttct tcaattttat ctacgctgac aggtcggaat gtacctaacc
    15421 ctacatgtag tgtcagatag actaattcaa caccctttgc ttcgatctca tcaagtaatt
    15481 ctttcgtaaa atgcaatcca gctgttggcg ctgcagcaga accattttct ttcgcataga
    15541 cagtttgata gcgatctggg tcttccagac gttctttgat atatggaggc aatggcattt
    15601 ctcccaatga ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca atgatccgtc
    15661 cgccgtgttc caattcttct ttcactactg cttgcaatct gccatcacca aaagagattc
    15721 ttgtcccaac tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg
    15781 tattcgttag aagaaggact tctaaatgcg caccagtatc tggtttttct ccatataaac
    15841 gagcaggcag aactcgtgta ttattcataa ctagcgcatc accaggctgt aactcttcaa
    15901 tgatttcatg gaaatgttta tcttccatct ctccagtttt ccgatccaaa acaagtaaac
    15961 gagaatgatc gcggtctttt aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat
    16021 caaaatcttc tgttgttaac atatcgtcac tcactttcta tcatgtgctt catcattcta
    16081 tcatgttttt cgttctgttg gcaaaatcag cactttttca cttctatttt ttgttttcct
    16141 aaataaaaaa actaccctat ttgtattttt tcattgtttt aggcatacta gtaatagata
    16201 gttcttcatt gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa
    16261 aatttataaa ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga
    16321 gttcatttgt tttatcggta cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa
    16381 ccgaatgact gatccaacac aagggaaaat tttgattgat ggaaaatcca tccaagaaat
    16441 tgatccagtg gaattgcgac gtcagatcgg ctatgttatc caaagtattg gattgatgcc
    16501 tcacatgacg atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga
    16561 agaacggaat aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt
    16621 agatcgctac ccacatgaat tatctggtgg tcagcagcag cgtattggag tcgttcgtgc
    16681 ccttgcagcg aatcaagata tcatcttgat ggacgagcca ttcggtgcgc ttgatccaat
    16741 cactcgtgat tccttgcaag accttgtcaa agatttacaa gagcgtttgg gaaaaacgat
    16801 cgtatttgta acccatgaca tggatgaagc gattaaacta gcaagccgta tcgccatcat
    16861 gagtgaaggc cgtgtgatca atttgataca ccagaaaata ttttacgtca tcctgcaaac
    16921 gatttcgtag aagagttgat tggagaagat cgcctactac aagccaagcc aaatgcgaca
    16981 agagtcggcg aagttatgct aaatacagca atcacgatta caccggaaaa atcattacaa
    17041 gaagcaatca aactgatgag ggaaaaacgt gtagatacgt tgttagttac cgataattcg
    17101 aatgtattaa aaggatttat cgatgtagaa acgttgaata aaaaacgagg aaaagtatcc
    17161 agtgtcggcg acattttgaa taaacatgtt ttttacgtaa aagaatccgc ctatcttcgt
    17221 gataccttgc aacgcatttt aaaacgcggg ctgaaatacg tgccggtcgt tgatgaacag
    17281 aacaaagtcg taggtatcct gactcgtgca tcattagtgg atatcgtcta cgatgtgatt
    17341 tggggagaag atcccgcaac tactgatgtt gcagagtcag ctactcctga agaatctaag
    17401 gaggtctgac ctatgcagca gttttttcat cagcacggtt cagaaattct ctcaaaaggt
    17461 tttgaacatc tttatatttc aggacttgct ttgatcattg gtatcatcat tgctgtaccg
    17521 cttggtgtct tactgacacg ttttccaaaa gctgccacga ttgtgattgg cttaaccagt
    17581 gccttgcaga caatcccttc tcttgcttta ttggcattga tgatcccgct atttggcgta
    17641 ggtaaattac ctgcgattat cgcactcttt atttattcat tattgccgat tttacgtaat
    17701 acgtacattg ggatgaaaaa tgtcgattgg aattatcggg atgtggcaaa aggcatgggg
    17761 atgactgagt tccaatccat tttttccgtt gaattgccca ttgctatgcc aacgatcatg
    17821 gctggtattc gtttagctgc cgtttacgtg attgcatggg ctacacttgc ctcttatatc
    17881 ggcgctggtg gtttaggcga tttgattttt agtggactga ataattacca accagatctc
    17941 attttggcag gtacgatccc tgtaacaatt ttagcactat tggctgattg gcttctaggt
    18001 ctcttggaac accggctgac accgatcgca cttagagagg agaatgaaga atgaaacgta
    18061 tactaaaaat gaccatggtt cttttctctc ttcttatact tgcgagttgt tccttccctg
    18121 gtcttgccag caatacagat gaagacacca tttcgatcac aggtggaatc acttcagaag
    18181 cacagatctt agcaagtcta gttgccggaa tggttgaaca ttatacagat aaaaataccg
    18241 caatcatcaa caatctggct actacaacta tcaaccatca agcgatgatg aacggagatg
    18301 cttcaatttc agcggctcgc tacacaggaa cagatttaac gactacattg aaccttcctc
    18361 ctgaaaaaga tccgaaaaaa gcgtttgcta ccgtgaaaga tgaatttgag aaaagatacg
    18421 gtcaaacgtg gttcccgtcc tatagttttg aaaatacgta tgtctttctt gttcgaaaag
    18481 acaccgcaca aaaataccat ctatctaaag tcagcgatct gaaaaatgta gcggatgaat
    18541 tagtagctgg tgtagatact tcttggatca atcgtaaagg agacggttat gacggcttcc
    18601 aagaaactta tggcttctcc ttcaattcga tattacctat gcagattgga cttgtttatg
    18661 acgcagtcga agcaggaaaa atggatattg ttcttggcta ctctacggat ggacggatcg
    18721 ccagttatga tctagtcatg ttggaagatg atcgtcaatt cttcccacca tatgatgctg
    18781 ctccagttgt agacaacaag atattagctg atacgcctca tttaaaagaa gccttaaata
    18841 agttaggtca taccatcagt acggaaaaaa tgcaggaatt gaactacgaa gcagataata
    18901 atctagtgga accatcagta gtagcacaac gtttcttaca agaaaaccac tattttgaag
    18961 ggaagtgaag caattggata aaatgaatgt ctttcaacaa tttttctatt atttccagga
    19021 aaatggctcc tatatctttg cacaatttat ccgtcacttc ctgatttcta tctatggtgt
    19081 cctctttgca gcagttgtcg ggattccggt tgggatcatg atttctagga gacgtaaatt
    19141 agcaaactgg gtgattcgtt tggcgaatat catccagacg atcccttctc tagccatgat
    19201 ctccatttta atcattggat taggcctggg agtcaatgtc gtgattgtta ctgtattttt
    19261 gtattccctc ttaccgatca tcaagaatac gtataccggg atgatccaag tagacaaaaa
    19321 catccttgat gtcggcaaag gcatggggat gactgctaga caacgactat tcatggtaga
    19381 gttaccactc tctgtttctg ttatcatggc aggcataaga aacgcgttag ttgttgccat
    19441 cgggataacc gcgatcggtg cattcgtcgg tgctggtggt cttggagata tcattatccg
    19501 aggaaccaat gcaacagagg gcacatcgat tattttagca ggtgcattgc caactgcatt
    19561 aatggcgatc atcacggact ggcttttagg tattttggaa agacggctag atccagccag
    19621 ccgtacatca cgctgatgtt attgtaaaaa acaaaagctt ctgcgaccat ttgttggtcg
    19681 caaaggcttt tttagttctg tcatttttaa ggcaataatc cactctgatt cttccctcct
    19741 tatgggcaaa gagttgcttt tcttagctat ttatccattc cgtatactcc aacttcatac
    19801 tgccaacggg ttcatatctg catccaccga ctaagttttt ttcttatccg ctaaggcatt
    19861 ttgccaatcc attttcccat cactcgtcat ggaattcata ccacatgatc tctaaatact
    19921 cgtctgcttg ttctttgaat tgggcatcaa ttcttacatc ctttggcgtg gctttcattt
    19981 aagccttttc catcagtcct cgattgttcg atgccttcag ctgcatcatc aaaactggtg
    20041 actgccgtat cggtaacttc gatcatcttt tatacaatcg ggaacttgcc atcctcatcc
    20101 ttggctaaaa acatatctag ccattccagc ttctcgccat gggaggtttg cggttcctaa
    20161 aaccggtaca cgattcttat ggactgcatc cgtcgcaact agacttggcg atacgatgat
    20221 ccttcaccag aagagcaccc ccgtaaacca attgatcgat atattgccaa taagaaaaga
    20281 cattggcgta aaagatatcg atccctcgtg atgcattgcc gttgatactg ctattcatgg
    20341 ccgaaagcgc cacgacttta acttcttccg tttgagtgac attcatgttc ggcaatgtcg
    20401 ctttggatac ccgctctgct aaaggaacga cactggtatt gtatttcgta tctgtatctt
    20461 ttccaaaaga ccatgccaac gaatcctctg ggaaccaata agacgactcc ggttgattgt
    20521 ccattgcttt tttcaacata cttttggctg tttctcgata aacgacattt ccaattgtct
    20581 tcgcctcact ttggcgataa tcgatggtcg ttgccactgc caacactaac aacgatgctc
    20641 caattttact gattttcctt gtcacacttc ttcctccttg ttttacataa acccgtcaac
    20701 attccattcc taaactcaaa caatgaccct tcaaaggtgg agatatagga acaaattaaa
    20761 gaaatcatgc aataaatcca agaaggttgg ttttagaaca aaaaaagagt ctcctttttg
    20821 gggagactct ttgttaacta tctaaaaagg ttggctcttc tcacttcact cataatgtgt
    20881 ccaggcagaa taaatttcta caactttatt ttcctcatca actttgtaaa ccactcgatg
    20941 ctgtctattc agcctgcgtg agtatctgcc ttcatgtgta ggtctcagct tttcaaaaga
    21001 ctgcgtgggc atatatggat cttccttcaa tgtctgaatc acttcttcaa attgtttctt
    21061 taaattcgtt tgcttgattt ttcttaaatc cactttcgca gagtttttga ttgcgactgt
    21121 atagttactc atcaaagagc ttcccaatca atatcatcga tattcgtaaa gctactgtcg
    21181 tctttttcgc ggtctcgtac tttatctaaa gtacctgttt gctcaagaaa aagggtttct
    21241 tgaatcgctc tccagtcttc cagcccaatc aatacagcat tgtttccact acgatcacta
    21301 ataatctcga tttcagtatg attttcattt acctctttca ataactgata aaaattttta
    21361 cgagcagtgc taggattcat cgttgtatta gccattgtct ctcctccttt ttctgatata
    21421 attatagcgt acttgattaa gtacgtcaaa aaaatacaat aaagcactta aattcagcaa
    21481 acttttcctc ttttttacat agagaaaaag accccttttt agggaggatc tttgtccacg
    21541 ctcacttctt actcctttac gactagcggt agccaaacgt aaagtaattg gtaacaacgt
    21601 atcatcttta gcaaaggtcg aatccaaagc ctgtgagtct atgcaacaac accaatacac
    21661 gaaggttcct cgttgcagtg tgacttgatt gatatcttga ataacctgag gatacgcacg
    21721 aaagatttgt aacggttatt caaaactgat tttggccgtc aagtcttcta ctgcctcaaa
    21781 aatgattgcc tctttcgtct cctgtcattc atgttcacta tcgatttgat acttgcttag
    21841 accgttgggt ttgtgaatca ccaattaaaa atggcactga gttatggtaa caaaaaatgg
    21901 tcacttgacg aggctgagat tcctacttat gaagacacat ggcacttaag agtcgaggta
    21961 aaggaagaag ctgaaaatat aggaggctgt acattatttg gcaaaggatt cggaaattat
    22021 gatcaagaca tcaaaatcaa aatgttttac agctaaatag gaagaataat aacatttcat
    22081 acgctgttcc catctttaat ggcttataga gggcaccttt agctgagaat aagacaaaac
    22141 agaccctcta gcttcaatta agagctagag ggtctgtttt tgattttaaa acttggttta
    22201 tttcttcaaa tggtagctat aggtgctgac aacgatattt tcacgccaag acaatcgcaa
    22261 tcttaaccgc aaacttgttt ggttatggag aatattctgc cagcgtctgt ttggtacaaa
    22321 ttgaggaatc aacacagtag ttgtatagtt ttgtttcgct gcatttttac ttacgatatc
    22381 tacataacgg ataattggat tttcaatcga gcgataagaa gagtggacga ttgaaaaacg
    22441 gacatctggg aaatgctttt taaattctgc ttcgatttct ttttcttttt cgacatcttc
    22501 gtctagcgaa acatgcatcg caacgacata atcaccgatc gaacgtgcgt aattcaacgc
    22561 accgatattt acccgtgtca cattcccgac taatacaaga acggtattcc cgtcataatc
    22621 atgtagtttt gcatcatttt ctaaacgaag ctgttctgct acttttttat aatgatcatg
    22681 gatcttataa aaaatgaaca taacgattgg cataatgatg aagaaaggcc aaatgtcccc
    22741 taaccgatag acaaacaaaa tcgcaatgat tgcatatgag atgaacgcac ccgtaatatt
    22801 ggcaatcgat ttgcttaacc atctttttcc ttcttttttc catttgacga ccatccctgt
    22861 ttgagataaa gcaaatggaa tgaacacgcc gatagagtat aacggaatca agcgttctgt
    22921 tgagccatgg aaaataaaca gcaatacgat cgaacctagc gctagtgtga taatcccatt
    22981 ggaatagcct agtcgatccc cacgatcttg atacatgtga ggcatgaatt tatcttttgc
    23041 cagattgtat gcaagaactg gaaaagcaga aaaaccggtg ttagcagcaa ctgctaaaat
    23101 cagcgctgta gcaaattgaa ggacgtaata caaaatacca tgtccaaaga cagctttacc
    23161 aatttgagac aaaacagtga cttctttttc aggaacgatc ccataccaat agttgataaa
    23221 tgtgatcccc acgaagaaga aacctaagat caatgccatc atcgtcaatg tagcagcggc
    23281 attttttgct cttggcttct taaagaatgg tacagcatta ctaatcgcct ccaccccagt
    23341 taaagaggaa gaaccagaag aaaatgctcg taaaatcaaa gcgatagaaa cacccggcac
    23401 agccgctcct ggaagtgcag tagcttgcaa tggcacagca cctgtcacga tattatataa
    23461 accaacaacg atcagtaacg agatcacaag gataaatgtg tacaccggaa ataataggaa
    23521 cgatgcagat tccctcagcc cacgaagatt cagcatcatc agtagcaaaa cgattgtcac
    23581 agaaatagcg acttgatgac cgtagagtgc tggcaccgct gatgtgatcg cttcagcacc
    23641 tgctgagaca gatactgcta ctgtcaacat gtagtcgatc aataaagagc caccggaaat
    23701 caagccagca ttctttccaa gattctcact gctgacgaca tatgctcccc cgccatgagg
    23761 ataagcatgg atgatttgac gatatgatag cgttaatgaa atcaataaaa taataacaaa
    23821 agcggcaatg ggtagcgaat accagatggc tgcagcagac aaagcgacta aaacgacaac
    23881 gatttgttcc gtaccatatg caatagatga taacgcatca gacgacaata atgccaaagc
    23941 agcaaatcgg gttaatttgt gctcatcatt ttcagcagat tttaaaggct tacctactaa
    24001 caaccttttt aagtaatcca atttctaaac tcctttacac acaattttac ttcaagtttt
    24061 atagtcgaaa atttatagta aacaagatgt tatagtacgc tcactgacct taaaagtcaa
    24121 taaactttaa gaaaataatt ggaaaaaaac agaaaaatat caagcgcttt ttcaaacgct
    24181 ttaatacagc aaaaatctaa cattttttta tgggcttttt ctttgaattc tataataaaa
    24241 ggatagaact tgacttagaa aatcaagttc tatccttttt gatatcttaa cttttggaag
    24301 agagatgtaa ggctgctttc tacccatcat tcttactact cgaagcatag cgcagttgtc
    24361 acaacctctt gataaacggt gtttaaaagc ttgcatctgc ggtaataagc ccaaccaaat
    24421 taaaaacata agaagctgtt tttatttggt cgttcttatt acttgatgca gagcagcttt
    24481 tttacaacct cttggtacac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaaa
    24541 cggcaaaata tgaggaacta tttttccatt tttttctcta atactcaggt caaaacactg
    24601 atttcacaac ctcttttttt aatgaaaaat atggctctca tgcgccgctt ctttgaattt
    24661 cgtcattttt tcttcgatga acggatagat tgccgttcct acgattccaa agacaacaaa
    24721 caaaccgatc atgatccaga ttgctttcgt agcatttggc caataaattc ctcctgcaga
    24781 ttcccgtaac aagtttaccg catgagtaaa tggcaagaac ggattgatca tttggaagaa
    24841 tttccctgag acttggatcg gatagttccc gcctccgcca gagatcgaca gtactaagat
    24901 gataatagcg atccctttac cgacattatc gaataatgca gccaagacat agacgatcat
    24961 catgaacgct aaggcgatca agacagcaaa tgacacgctg tagaccggat tttggacatc
    25021 tactcctagt aagaaaatat tccctaatgt aacgatcaag ctttgcgcaa tcgccattgt
    25081 caaaaatgtc agcatacgag caacgaattg ttctcgtttc gtaaaattgt ttcggtcttt
    25141 ctttcctaaa tagtattctg tcgtagcaac acttgaaaga agaacagcgc cgacccacag
    25201 acatagtgca gtataaaacg gggtgctagc tgaaccgtta tttgcgattg gataaagcgc
    25261 attggtttgt agctcaacag gttgtgtaaa gaagtcactt tcttttgttg cgtctagttt
    25321 caataatttg atgacttcgc ctagatcgac ttcttgttcg ccttttcgga tcgcgtctgc
    25381 tgctttttgg ataccagctt tgatatttgg ccagtcattg ttgatcaagt ccgcagcagc
    25441 gttcacagct gtttcgactt ctggcatttt ttcatttact gttgtcaatg taccggtgat
    25501 ttcatttttt agtgctggcc aatcattttg gataaaatca gcggccaatc ctaatttttg
    25561 ctgaatgatc ggtaattcat tgttgtatag gtctgcccct tcattgatgc cattgacgat
    25621 ggtatccata tttccattca gcattgtatt ggcatcatgg atttcttgtc caattgcagg
    25681 catttctttt tgatattttt ctaaaattgc cactgcattc gttacagttg ctttcgttga
    25741 gctgagaagc gaatcaaagt cgatctggtc cgctttgctc agtgcgcctt gtgcagtccc
    25801 aatcgtatcg atgacttggt ctagtagaga agtgacagtg gaactgattt gacctgtatt
    25861 gataccagca atgatgccat tgatattatt aacaacggca tcaatttgat ctagcaattc
    25921 atcaatagcc ttcgtgtctt cattttgaac agctgtgttc aaagcagata ggcggttttg
    25981 cagattgctt agcaagggtt ttaattgatt tagctgatca ataattggct gtaatttcgt
    26041 atttccagca cttgtttgca atttagtcag gaactcgacc agtttatcga ttgcattttg
    26101 ttgttgagat attccgtcaa taaagccgtt caatacattg ctgatgtttt tcttttcatc
    26161 atcagtcagc ttaccatctt caatagctgt tcttacagta tctattaacg aacctgtcgt
    26221 tgttgccact tgtccaattg acttcaatgt aacatctaca ctgtttgtga tgcttggtat
    26281 cgcatctttc agctgagagg cacctgattt tgtttggttt gctagatcac ttgcctgatt
    26341 ttctaaattt ttcacatctg gcaagatatc ttgtacttgt tggatgatcg tcaagccttg
    26401 ttttgcttca tcgattcctt ggttcattgt ctcttggatc gaagcaaaat cgccgtcgat
    26461 ctctgctagt tgcttaccgg cattttggat ctctgggatt ttttcttgca aatcaaggat
    26521 gatttttgct tgatctttca actcaggcat tttgtcattc aatgcaacga ctttttcacc
    26581 catctcatct actttaggga tgtaatccac gaattcattt gctttattca gtttttcctt
    26641 gatttcaggt agttgacttt gtaattcgag gacttgtttt gtgtagccgt cgatcgtgtc
    26701 caaattttca tctgttgata aaatcatatc tttaacttta ttgatactta ctaggttcgt
    26761 atcgatgtcg taacctatct cattgaatac tttcaataaa gtcgaactgg ctgttttgat
    26821 aaattcattt gtgatttgcg attggatcga acttgctcct ttatcggtga ttttcggtgc
    26881 aatcgcattg attttttcat ttaccgtata ttcgattttt ggtttcttga tatcaccact
    26941 agtaaaactc agcagatctt ctgagaaatc ctttggcaag taaatacctg cgtaatactt
    27001 tccggaacgg actccgtctt ctaattgttc ttttgaatca acgaattgcc atcctaattg
    27061 tttattttta tgaagcgatt cgatgacttg ctccccaatg gccacttctt taccttgaaa
    27121 ctctgctggc ttgtctgcac tgtaaacagc aattggcagc tctccagtat ttccatatgg
    27181 atcccataat gctttgatgt taaaccacgc atatagcgaa gggatgatca tgatcgcgat
    27241 gatcagaagt gttgcaatcg ggtttttgaa aatccgtttc cagtcaagca aatataatcg
    27301 aagcgtattt ttgattgact gcatagttct cccccttctt cttttttcag ctgaattact
    27361 ttaccatgta tttcatactt ttttcaatcg gcttttttca aaaaagtcga atttttggac
    27421 atatcacggc aattgtctca ttgtaatctc ttacatattg agaaataatt aaggattaca
    27481 gcctttactt tatcatgaaa taaaagggaa agataatttt atgccaaagt ttttatctat
    27541 tatacgtata cgcaaaaaag ctaaaaca
//
LOCUS       DASNNU010000031        26602 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.031, whole genome shotgun sequence.
ACCESSION   DASNNU010000031 DASNNU010000000
VERSION     DASNNU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.031"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2611)
                     /locus_tag="U8116_001241"
     CDS             complement(<1..2611)
                     /locus_tag="U8116_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN8434248.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGT"
     gene            complement(2608..3312)
                     /locus_tag="U8116_001242"
     CDS             complement(2608..3312)
                     /locus_tag="U8116_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN8434249.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(3324..5633)
                     /locus_tag="U8116_001243"
     CDS             complement(3324..5633)
                     /locus_tag="U8116_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN8434250.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5828..6292)
                     /locus_tag="U8116_001244"
     CDS             complement(5828..6292)
                     /locus_tag="U8116_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434251.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(6292..6918)
                     /locus_tag="U8116_001245"
     CDS             complement(6292..6918)
                     /locus_tag="U8116_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN8434252.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6925..7290)
                     /locus_tag="U8116_001246"
     CDS             complement(6925..7290)
                     /locus_tag="U8116_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN8434253.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(7280..7618)
                     /locus_tag="U8116_001247"
     CDS             complement(7280..7618)
                     /locus_tag="U8116_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434254.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(7608..7934)
                     /locus_tag="U8116_001248"
     CDS             complement(7608..7934)
                     /locus_tag="U8116_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN8434255.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7912..8193)
                     /locus_tag="U8116_001249"
     CDS             complement(7912..8193)
                     /locus_tag="U8116_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN8434256.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(8195..9559)
                     /locus_tag="U8116_001250"
     CDS             complement(8195..9559)
                     /locus_tag="U8116_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN8434257.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(9572..10141)
                     /locus_tag="U8116_001251"
     CDS             complement(9572..10141)
                     /locus_tag="U8116_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEN8434258.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(10113..11300)
                     /locus_tag="U8116_001252"
     CDS             complement(10113..11300)
                     /locus_tag="U8116_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN8434259.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(11364..13091)
                     /locus_tag="U8116_001253"
     CDS             complement(11364..13091)
                     /locus_tag="U8116_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN8434260.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(13088..13540)
                     /locus_tag="U8116_001254"
     CDS             complement(13088..13540)
                     /locus_tag="U8116_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEN8434261.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(13652..14032)
                     /locus_tag="U8116_001255"
     CDS             complement(13652..14032)
                     /locus_tag="U8116_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN8434262.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(14029..14415)
                     /locus_tag="U8116_001256"
     CDS             complement(14029..14415)
                     /locus_tag="U8116_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434263.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(14417..14695)
                     /locus_tag="U8116_001257"
     CDS             complement(14417..14695)
                     /locus_tag="U8116_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434264.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            14743..15471
                     /locus_tag="U8116_001258"
     CDS             14743..15471
                     /locus_tag="U8116_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEN8434265.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(15617..16093)
                     /locus_tag="U8116_001259"
     CDS             complement(15617..16093)
                     /locus_tag="U8116_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEN8434266.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(16053..16400)
                     /locus_tag="U8116_001260"
     CDS             complement(16053..16400)
                     /locus_tag="U8116_001260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434267.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(16387..16572)
                     /locus_tag="U8116_001261"
     CDS             complement(16387..16572)
                     /locus_tag="U8116_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434268.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(16573..16845)
                     /locus_tag="U8116_001262"
     CDS             complement(16573..16845)
                     /locus_tag="U8116_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HEN8434269.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16842..17045)
                     /locus_tag="U8116_001263"
     CDS             complement(16842..17045)
                     /locus_tag="U8116_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434270.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            17170..17382
                     /locus_tag="U8116_001264"
     CDS             17170..17382
                     /locus_tag="U8116_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434271.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(17415..17609)
                     /locus_tag="U8116_001265"
     CDS             complement(17415..17609)
                     /locus_tag="U8116_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434272.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(17622..17804)
                     /locus_tag="U8116_001266"
     CDS             complement(17622..17804)
                     /locus_tag="U8116_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434273.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17801..17941)
                     /locus_tag="U8116_001267"
     CDS             complement(17801..17941)
                     /locus_tag="U8116_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434274.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(18431..18796)
                     /locus_tag="U8116_001268"
     CDS             complement(18431..18796)
                     /locus_tag="U8116_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434275.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18836..19138)
                     /locus_tag="U8116_001269"
     CDS             complement(18836..19138)
                     /locus_tag="U8116_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN8434276.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(19138..19437)
                     /locus_tag="U8116_001270"
     CDS             complement(19138..19437)
                     /locus_tag="U8116_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434277.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(19430..19594)
                     /locus_tag="U8116_001271"
     CDS             complement(19430..19594)
                     /locus_tag="U8116_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434278.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(19600..19869)
                     /locus_tag="U8116_001272"
     CDS             complement(19600..19869)
                     /locus_tag="U8116_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434279.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19881..20630)
                     /locus_tag="U8116_001273"
     CDS             complement(19881..20630)
                     /locus_tag="U8116_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8434280.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(20627..21319)
                     /locus_tag="U8116_001274"
     CDS             complement(20627..21319)
                     /locus_tag="U8116_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434281.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(21325..21996)
                     /locus_tag="U8116_001275"
     CDS             complement(21325..21996)
                     /locus_tag="U8116_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEN8434282.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21989..22330)
                     /locus_tag="U8116_001276"
     CDS             complement(21989..22330)
                     /locus_tag="U8116_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434283.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(22508..23206)
                     /locus_tag="U8116_001277"
     CDS             complement(22508..23206)
                     /locus_tag="U8116_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEN8434284.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            23293..23763
                     /locus_tag="U8116_001278"
     CDS             23293..23763
                     /locus_tag="U8116_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434285.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23806..23985)
                     /locus_tag="U8116_001279"
     CDS             complement(23806..23985)
                     /locus_tag="U8116_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434286.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23972..24310)
                     /locus_tag="U8116_001280"
     CDS             complement(23972..24310)
                     /locus_tag="U8116_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEN8434287.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(24326..24529)
                     /locus_tag="U8116_001281"
     CDS             complement(24326..24529)
                     /locus_tag="U8116_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8434288.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(24544..25320)
                     /locus_tag="U8116_001282"
     CDS             complement(24544..25320)
                     /locus_tag="U8116_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEN8434289.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            25619..25939
                     /locus_tag="U8116_001283"
     CDS             25619..25939
                     /locus_tag="U8116_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8434290.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            25957..26388
                     /locus_tag="U8116_001284"
     CDS             25957..26388
                     /locus_tag="U8116_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN8434291.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT         7497 a         5520 c         4120 g         9465 t
ORIGIN      
        1 aagttccatc tgcaaaccat gaacccactt gtccacctct tgcccaatta ataattgcac
       61 caactactaa ttgatcatat ctagggtttt gaatcacttt ccaaccaaca gcagaccaat
      121 cataggcaat accaatatca gatgctgcag aagtatttcc tatcacatga gttaaaccat
      181 attttgttcc agcacccatg ccacaaccac ccagatatcc tgaatattct gcagacaagc
      241 cataacactg accattgcca attcgttgtc caatcaaaga ctctaaatgt tttagtcctg
      301 cttctccagt agctcctcct ggttttaaat ctttaaattt gttataccag ttaactgcat
      361 aatcttgacg ttcaggatgt gtcgctgccg gacgttcata gtttcgttca aaagcatatg
      421 ctgcttgtct tggatcagta caggccttaa atccatcaac cgtcgtaggt tgtactacgc
      481 ccatccattg tccatttgta aatgtccaaa taagcaatcg aacttgtgca tctaagctcg
      541 ttattggttc tttaatacct gcagcattaa ataagttttg aacataaact tttccatccc
      601 atgttgctgg accaacaaga ggatatgaag aaccatccca ctgaactaat ccgtatgctg
      661 gtccacctat ttgaacagta tcaggatcaa aagttcctcc tgtttcttgt tgaatgttcc
      721 ccaatattcc acatgcagat tgtttcgtaa atccgttatt acacaaaata tcgtatattc
      781 tccaagctct cttttctgca tctgttttta attcatcagg atacccacca ggtgtttctc
      841 ctccgccaga tggaccgcca ttttgtcctg gtatcacttc tttacctcta acagtaagtt
      901 ttccctgaat atctaaatca ccgaaataat atgcttttcc gtttcctaat aacacaaatc
      961 ctttttctgc agttggagaa attaaaatgt attttccatc gccatttgta cgtattacca
     1021 aagaattttc ctcaagaggt gtaggtgtta cagcatttgg aaatggattt cctgctgaat
     1081 ctgttgttcc gatcgttcca atggattctt ttctattcca gaattccata ccttttttgg
     1141 ttagctccat gatttttttg ttttcattcc aaatttgaag cgtgcctttt acaagtttta
     1201 acacatcacc ataagcatta aaggaagttt cgaatacttc tgcatttatt gttcctactt
     1261 tgatgaagtc agcaacaatt tctccttttg atgtgatagc aataccgaac gggccattta
     1321 ctccgttgtc tgaataacct aagccattta aattccaacg ccacactcgc tttgcattcg
     1381 ccacatttgg agtatccatg ataagaatct ctgatggagc tttttctgga cgaaaaacga
     1441 catgcccacc agaattacca gtgatccatg ccgtcgcatt caacacattt tgaactaacg
     1501 tttctgttcg attgtcgatt ttctttttca attcttgtgc ttgagtattt actgccgaag
     1561 tgtacagtga taaatcatta cctaaaacaa tatccttata tttcccaagc gttgggaaat
     1621 aagtgtattc gaccatacgc tcttttatct cgatatctag ctctttcgcc ctaacatgag
     1681 cgacatcacc aaagtgtaaa gacgctagct cttgatacat gtcaccgtat tcgagtgtgt
     1741 gttccaacgc caccatactg actgtatgag tagcttttgg ctcatggata cgatcattgt
     1801 caaataaggt tttgccccat ttgatcaatt catcaactgt tttacaatct ccgttttcac
     1861 gtttggcaat acgtcgattc tcgttggtta ctccatcaat ttctaaataa ccatattcga
     1921 taggttcttt gtcttcatca taatcgttat caggaacacc gccaaccaaa tacaaactat
     1981 tgacaattga ttcttcatca atttcttctt cgattgcttc taaattgatg ccaaaatcaa
     2041 tccgaaaacc attatctgat ccaatctgtt ttaccagttt caaatcatag ttatccatat
     2101 caagttccgc gctagtcacg ccagtcaaat tttgattacc gttatttgaa ccaatgattg
     2161 cttcaactgg cgcgacttgt cttgctgtaa attggtgcgt tgtccctaca ttcgaaagat
     2221 agttaaattt ttgatcaaat gccaaactgt ttttaagatt ggtcatgatc tgagacccat
     2281 tgccgttatc cgtaaacgac ttgacaataa agttcttatt ggccataaaa ccaatatgcc
     2341 gagctgttac agaaacggag gtcaaattct ttttgacatt ataaatttca aaatattgcc
     2401 atgatccatc tggtaccttc gcttttataa aatttccttt ttttaaataa gagcgatact
     2461 gtccgcttct tgaatagtta ccataaaaac gatattggcc attcagcaca cgattgattt
     2521 ctggcaaatc ttcccaatca actaacgacg ctccgttcac gctcaaatca tctggcattt
     2581 ttttataagc ataaataaat tcttgcgtca cagatacaca ctcctattcc aaaaccgcac
     2641 ttctttaaat tttccggata tttttacctg attccattca ggctgcaata ctggccaatc
     2701 accacgcgta aataaattca aaccttcttg tattgctttt ccaagctgag tatcaaccac
     2761 gattgttgca gctaacgtat taagtattgt taggctttta tcaccgacag cgatcgttat
     2821 atctcctccg ttcgattcta tttccagata gggatgtgca atttcgtcac catgatcaaa
     2881 aatatccata acactagaat gaaaaatttt aggtgcttca ccgatttttc ttttgagtgg
     2941 ctgacaacga aaagtaacat cgaatgtata aaagaagccc cactcgtttt cgaaagggac
     3001 ttctttatcc atgctgcaga tagcctctag atacttgtct ggatcattat gtgtgattaa
     3061 ttggctttta ccagtgagcc atcgtttgac ttctctcagt ttcgaatgtg gaatagtgat
     3121 cccttctatt tccaaatcaa aaggttcata gtcatcaaac gtctccgtca attctccgct
     3181 tcggccctga atcgtgtaag tttcgtatcg cttgttcggc ataatatctg gaagctctgc
     3241 ctcaatgatg caattcatat caattactgc attccgattt ttccaaataa aatttggttc
     3301 atctggattc ataaattgtc gactcaaata ggaacacctc ccaaatcacg aatagcctgt
     3361 ttgtttgctt tagcaaattt acgattcata cgatctaact cagatggatt gtttgcatcg
     3421 actttgccaa tgtgtatatg ctgttcaatg ctacctccag aaactcttcc actgatccct
     3481 ttgcgttttt cttcgtctga taatggagta actgtggttt taccattctt ggcagtcagt
     3541 aactcaggtc cagcttcacc aacaatcgct tgtccattga ttagatgacc gccttgtgct
     3601 aaataaggca gttttgaaat agaaaatgat tttccaccta caccaggaac ccattttggt
     3661 atctttatat tattcaatcc accaataaat ccattgatca atccaatcat ggcattaata
     3721 ggagctttcc ctacagcaac aataccatcg aaaatacctc caaaaatatc aactagacct
     3781 tgccatgctt ttgaccaatt ccctgtaaat actccagtga taaaatctag aaaaccactg
     3841 aaaattcgtt ttccagcatt ataaaaatta ttgaaatttg ctataacacc atcgaaaaat
     3901 ccaccaaaat aaccacttaa aaaattaaat atttcaacag ctacatctga aacacctttg
     3961 aagaaagaat tcactccatc atgaaaccat ttaacgttat tatatgccca aattagtcca
     4021 gtaactaatg cgccaatagc tataactgct aatgcaaacc agcctcccga cattccaaat
     4081 aaaccagcaa gtccttgcca aactcctata aaacttttaa cttccccaac gatttttgta
     4141 acagatccca taagtgaacc aagaacgatt agtactggtc caactgctgc cgctatacca
     4201 gcaattgtta tgatccaatt ctttgtatct tgatctagag acccccacca tttagagaat
     4261 tcctgtaata aggaagttgc tttttcaaaa aaaggcaaaa ggcttacttg gactgcttca
     4321 cctacgtcag ccattgctaa ttttgcattg ttcatagctt gatcggcttg atcaattggg
     4381 tcgagagtag catcgaatgt gtctcctaca gctcccccac tttctccagc agtttttgct
     4441 aaatcttcca ggttcaaagt acctctacga attgcatcgg ccattctagg accgccttta
     4501 gtcccgaata cttctgctgc tgcattgatg gcttctgttt cagaacctgc attttttacc
     4561 ttgtcttgta gttcttctag tccttgactt aaagattttc cgtctttcgc ataagccact
     4621 gttgctttag ataaactact taaagctgcg cttgaatcaa caccagattg ctcaaattgt
     4681 ccaagtaaag tgaccccttc accaaagctc aaccctaatt gtttaatttg tggtgcgcca
     4741 tcaattgctt tttgcatcaa gtcgtctaca gattgaccag tattctgcga tgttttcgtt
     4801 gttacatcaa ggacagaatt caaatcatca tattctagtc cataagcatc aatagcttgt
     4861 cgagcagata ttgctgattg cgaaacatct gtatcattga tttcagcgta ctgtaggaga
     4921 taattggttg aatcttccag ttttttatcc atgaatccaa attgtgtatt tacctcacca
     4981 atagcttccc caaccgtttg taaaggtaaa tgagtatttg aaccaacgtt tttgaaagac
     5041 tgtgaaagtc tatcagcttg atcacctgtt gctccggttt ttgtaatgat ggtatcaagt
     5101 gcctcgtcaa cttcaccaaa tgctgcaagt cctgctgctc ctgccgctac aataggcgcg
     5161 gttactccaa cagacatttt ttcaccaacg ccttttactt tctcacctgt ttcttcgatt
     5221 ttctggagtt tttttgctgt atcaacagaa acgtcacctt gttctttaag tgcatcgttc
     5281 gtctgttcta atgctgtacg aagtttattt tctccggttt cagaattgag taattgttta
     5341 taaagttttt ctgattcagc agaatattca ccggtttctt tgacagattt ttcatattcc
     5401 tctctcagca tttgagatcg ttgttcagcc aatcctaatt gtttttctaa tttttgttta
     5461 gccgctcgca atttttctgt ttgagttgca tccttgtcca tcgcagacac ttggtttttg
     5521 tactcggcag cggctaagtt catttctttg ttgatttctt tgattgtttt cgaataactg
     5581 acttcgccat tcgttttaaa attaagaacg acatcagatt ctttaccagc catttatctt
     5641 agcgctcctt tctaccacca aggcgattta tccatagtta cagattgagg tggttcaaaa
     5701 tcggtgtttt gttgtaacca ctgtaaataa gatttgagcc acaagttagg tgtagacttt
     5761 aaaaagaaac tctcactcca attcaataga gtgagagcaa cgtaaatata aaaactccaa
     5821 ggagttccta tctcttccga ttctttttgt ttttctttct ttttgcttgc gctttttgaa
     5881 agtcttgtgg cttcttggat ttttttaggt cttccacctg aaatgtctgg ctagcaaaaa
     5941 tttccataca ggtactataa gcagacaaca cctctccact cattcccaaa aatttgaaaa
     6001 tagtttctgg atcttcctct aaaccaccag tacgcagcat ggcataaatc aaggcacgca
     6061 tgatttttag atcactaggc gataagttag cagaagaaat ttgtccttct tttttagata
     6121 gcatggcgtt catatcagat tcaaattgcg aataatctcc accatacaca tctgctataa
     6181 attccatcgt ttgcatagta aatgaaatag ggaattctgc gccttgaata attacaacag
     6241 cagaattttt taagtcttca acgtgaattc cataatcaga taaccgagcc attaacccgc
     6301 acctccaact ttagccaatg ttttccactg ttcttcatcg tatacaggtt gagcaatgaa
     6361 tttattaaat aattctagag aagctccgtc tctatttgaa tcaaaacttg catacatcac
     6421 attattgtat ttcagtccga tggaaaccag atttgcagtt acatcgtcaa tttttgtttc
     6481 ttcctctgca gtggcatact cttcgtcaat cacattggat agttgtgtat taggatacca
     6541 aactgctttt ttaccgccat tttcaatatt tccaataaat ccaaaggcga agtacggaaa
     6601 ttctcgtgcg gtatttttgc taaacgttac tccgctttta gcaactaatc ctttgatttc
     6661 atccatcact tctatcggaa tacccacgtg atccaacgca agttcatgtt ttgtttctcg
     6721 cgacacacga cgaaacattt tgctggatgc ccatttttct aatgctgttc cattcccttt
     6781 gacaccgatt tttgtggcaa ttggcaatcg taaaacttca ctataagtcg gtgcagttcc
     6841 aacagaatct ggcgttgcca tcatcgcaat taaaatgtcg tccaatcctt caaaataaaa
     6901 agtatcttgt tttcccaatt aaaatcaccc ttcccataaa tcaagtattt ttctagtcat
     6961 aatttcttca attttttctt tgttttgttc atacgtacca cttgcaaaat gttgggcacg
     7021 ttgatttgtt gtgccatttt cggcaaagcg ccagtaaaag gccgtctctt caaattgaac
     7081 tttcacttga tcgttttcaa ctactacttt tatttgatcg cgcatatgtt tcttttttaa
     7141 gagcgactta ggaatattag gaagcaactg ctttagataa aagttagctg cttcttctaa
     7201 agattccaac gataattttt tcggatcaac ttttgaaagg tttcctaaat aatccgatat
     7261 ttcagaaaaa ccattcttat tacttggcat tttccacaca cctcacatat gtgtaatagt
     7321 tggttacggt atcgtcgttc tcatcacctt gtatacctgt aaaatctgaa tatggaatgc
     7381 cagcattttg cagcgcttgt tcaataacaa ctaaatcctg ttctgttcca agtgtaaaaa
     7441 aagagacttg gtaataaggt aattttttat agactttacc ggatgccatt tttttgctgt
     7501 tactcacatt tgagtacaca atgtatggat acatcgtccc taatctggct ttgtctctga
     7561 acactgctaa ctttgttgat ttcagcgctg ttttcaattc atcaaagcta atcgacataa
     7621 gctaaactca actccatttc tcttgcatcg ggattcgtat aaatgcgagt aatgttatac
     7681 gttacagaat caattttgag cgcacttaat ttctctgtga tggatttatc ccatctgact
     7741 ttaattcgtc tgacaacgtc tgtcttggct tgctgtgata aatatttttc ttgagaagtc
     7801 acaccgagtt cttcatagaa tattagacgc ttaaattcgt aaattgtagt tggacgatcg
     7861 tttccgtctg ttcctgtttt gatgtctagc aattcggctt tccatctgag attattggtc
     7921 tgtctcttcg gcattttgaa tcgctccttg cacgataaat ggcgtcatgg cattcatagc
     7981 tttgtcgagt tcatcctctg aaactctata ttcataggca atgccggcaa ccatcaaaat
     8041 aagatattct tgttggccac cagttgctgt tttgacataa tcttttgcca tatttaaata
     8101 aaaagagagc aaagaatcat ccatgccctc ttcaaaatga atatgtgatt tgaatttttc
     8161 ctctaaagac aattctttag tttgctcttc catcttaacc accaactggt tttgtaattt
     8221 cgtagcgata tactgccggt tcaaatggag aataaaccaa ttgaccatct agcaagttgt
     8281 aaatttggaa tccaatttga tttttaccag agaatttttc aacaagtttt tgaatttcca
     8341 aggcaccaat aacttcttga attttaaatg cagaaaaatc gccaaaatat aaaactggcg
     8401 tgtctggttc acccttttta tctgctgcat ctgtccaatc cacaggatag ccaactaatt
     8461 ggtaaccaat tccaccttct gcttgtgtga atggccgcaa caaaggaaat ccatcatctg
     8521 ttttcatttt ttcaatggca gtcaaagcag ctcgattaat aataaagcgt ccctttttca
     8581 tcacttctgt cactggtgta tttttaaatt cgattaatgc atcatataat ttttgcccag
     8641 cacctgaagc agttagatct aaaggttttt taaatgctac agcctttttg gctaatgcac
     8701 caggattttc atttccagcg tcatcaccat tgaacatata attgatttct ttacgcacat
     8761 aagctttttt caattcttcc acaacaatat cttcaactgg aacaccagac atttttaata
     8821 attttttagt tactgttgcc aaagcatcga attcggcagg attaagcaaa atttcatcaa
     8881 actgaatagc tgtttcagca atatcagttg aacgctcttt cttgtttaca ttcgcatctg
     8941 ctttcttcac aagaattgga tatttgacat ctcctgatgt tcgcaccact gttccgtatt
     9001 tacgaagtaa attttcttct tgagcataag taataacttc agatgcaatt acttctggga
     9061 cagtaactga accgttgcca gcttcaatcc ctaaagctcg agcttctgct tcagaaatat
     9121 ttccagctac aaaattagca aatgcttttc gtagttgttg atctttttgt ttcttggtca
     9181 tttttgcacg tgcctccaat ccgttcttaa ttgatccaag taatccatct cgttgctgtt
     9241 gagtaatcat tccagaacga ttttctgaac tatcttcact gtctgaatct tcttcttgat
     9301 cgttgtcttc accagaacgg ctttcgtctg aatccgtact attcgattga tcatctgtat
     9361 tgtcgtctgt ttcatcagtt ccagaatctg cgcctaattc gtctttaatt ccgttcaatt
     9421 catcaataac actgtcaatt tcttctttca cggcttctaa atctgcttcg cgtaattctc
     9481 ctgattcaac tttttcacgt aattcagtca atcgttgctc acgacgtgcc ttcatttttt
     9541 tcaataattc tttatccatg tatttttcct cctacgcttc tagcgcttga ttaatttttt
     9601 tgattaattt ttttctttct tcgacgtttt tttctaattc atcacgactt cttaatgcag
     9661 cttctgtatc ttcgtatgct ggcaaaggca caatagaaac ctcgtataat tccacttcat
     9721 ggattgttcg aagcattggt tcagaattgt agtcccacgt ttcttcagtc ggcacaaaac
     9781 caaaactaca ttggttgatg tctcctcgtt ccatagattt aactaagtcc cttgctaccg
     9841 ttgtgtccgg taaatcaact tcgaatttca aaccacgttc atcttcttca aggcgaaggg
     9901 ttccgctttt agtacgtcct agcacattgg accaatcgtg attgaacaaa caacgaacat
     9961 ccgaattact aattgccttt gagaatgcac caggaacaat cacttcgctt aagtcatccc
    10021 ataaaagtgt ttgactgttg aatacagcag cgtagccgct aatggtcctc gtatgagttt
    10081 cttcatcgga acgtgttgaa agattggtga tgtcaatcgt gcgaatttcc ttcttcttca
    10141 tttccatcac ctcctttcaa gtcttgatca ttcgttggta aggaatcatc tgttgcattt
    10201 ttctggccaa tcctagataa gtcatttgaa atatagatag cttgtgtttc tggagtattc
    10261 tgtttaggaa aaccaagcat ttctgccacg ttatctggac ttgtaatccc ggtacgaacg
    10321 atgttgtagc caatatttgt ttttgttgag taaggaacaa aatccaaaat attaattttc
    10381 cattccactc tatagccaga attaggcata aaaaaaagag cggtgtaatg ttcgctcttg
    10441 tttttcaata ttggtttgat tgctttgttg tgcagataca tcatcgcttt ttcaatatct
    10501 gtcttcatca gcgattgata cgtatcaaca ttgattccta gaaattttcc taagtctttt
    10561 ttgtaaacac ctaaataatt caaaattgcc gcgtcatcaa caggactttt taatgtctcg
    10621 atggaatatc cttttcccaa aggaatcatc ttaacagaat gatcactctc attttgcgtt
    10681 ccttccagtt gatccaatat agctttgaca atttttgttt gggcgctatt atttggattg
    10741 atgtgggcgt ctagtttaag catgaacgca agtaaaccgc ctttagtata tttgtccgtc
    10801 aaaacttttt cagcgctcag aacgccttct agagtgtttc ttgcaagatc aattattcca
    10861 gcacctttta atgaatcagt tccgatgttc ttaatgtgtc gaatcatttg accaggtatt
    10921 ttttgaccat tcatttcaaa ttcttcttga agtcgttcat tgattttagt tgttacaccg
    10981 tacgccaaat gaagctgatc cctatccgtt aatgggaatg tctcaccatt gatcaataaa
    11041 gtatttgttt ccaatttggt aaattcgaat cctgtcaaat aatcattggg actcttcaaa
    11101 atatttagta agtgatgatc tttgacttcg ttgccgtcgg gaccaataac aattggtgtg
    11161 gccaatgcca cctgattaga tatgtcttga actaattcat aaacatcaga agattccatg
    11221 atagatgaat ccgtcacata tctttgacca taacgtgtgt agtgtccaaa aatatcctcg
    11281 atatatccac gtttttccat aaaagaatag actgcattcg ataaccgatc acgtaatttc
    11341 aatttttttc accgcctttc tatttatcta tagatggaac ttaggtaatc atctaactca
    11401 tctgaatcaa tatctgtcat ttgattcatc gtttccttat gaccacacaa aaacgccacg
    11461 aacccatcaa tctttttctt tgattgacgt ttacttggcg ctttttgtcc attgatgtta
    11521 gtaattgcta caacgttcaa agtgcaataa aggaataatg gattatcaaa ttgaattcgt
    11581 ttctcataaa acaaccgttc gacatcgtca aaaggagcat tcaatacttt aggatattga
    11641 gcaacttcaa cgcattccaa tcctaagttt tctaatttct caacaagttt gtcactcatc
    11701 gctggatcat aattcacttg ctgaatatca tataaattca tgcagtcctc tatgtactgc
    11761 aaaatctgat cttgatcaat catttttccg tcgcaaaatt caacgaaacc ttgttcagcc
    11821 aaatcactgt aaggaacatt atcttctttt tctcgaaact ctaaatcttc attcggaata
    11881 aaataaagtt gctttacttt aagaaccgct ttaccatctt catcccatgt tgggaagttt
    11941 aaagatacac aggtcaaatc tcgtgtacgt gataaatcta aaccgatata acatggttcg
    12001 ccacttaaat caccaagttc ttgtgtggta actaaacatg gctctacttg atcctgttca
    12061 aagaaattat ccgcgccgtt cacaaataca tccaagtgtt tcgttagaaa ttcagctttg
    12121 gaatgagctg accgttgcgc tgttttaaat gccgattcta aagcagataa atcgactgat
    12181 attccccagt taggattgca catttcccag acttttctat ccgtccaatc ataattttta
    12241 tttggctcat agattagaac aaagtttgaa tcattatcat cacgtttcaa cacttctttt
    12301 gcttcgcgat atacacgcat tccaacagac gacgaacctt tcccagcagt tgagatatta
    12361 aacatcaatg gctgtggtaa agatatctga gcagacttaa aattgtcata ctgttccatt
    12421 ttctcctgtt tatgcaattc atcgttcaat acaaaatatg gattggatcc ctcgatatta
    12481 tcaatattct tggtttgaac aataaattta tttgagtaag ccatatcttc atgaatatag
    12541 tcataagtaa tactagaaac agttcccttt ggccccctaa atattttagt tccttcaagt
    12601 aggattggat tgtttaagat tgtaactgca aaaggtttgg cagcatattg cgcttgagca
    12661 aaatcagatg cgcaagcata gcaatcaact gataacgccc cttctccgta cattgcatat
    12721 cctaatgaac cgacagcaat caatgtttta ccattctttt ttggaatctg gacataagcc
    12781 tcacgagtaa cgcgtacaat ttggcctttt tcattttctt taacccaacc ataaatccaa
    12841 gaataaataa atttttccca gggttctaaa agaaacggtt ttcccaccat ttctcctttt
    12901 gtgtgaacaa taaatgattc tacccaatcc atcatttcat tcgcgcgatc tacatcaaac
    12961 caaatatctt ttcttttttt ccactgatac cagcgatcta ttgctaaacg aacggtctta
    13021 ggatacttct taggtttctt tcttacttct tttgcaaata aatcagcata atttacacca
    13081 ggttcaatca tttttcagca cctgccttct tacgccattt atttctgtgc gctgctagtt
    13141 catctactgg ctttacttct gggcgtgtaa tttcttcatc ttttcttgcg gtcgatcctc
    13201 cagcaattag tctaccagtt tttgctttgt ttgttaatcc taataaatct agagctttca
    13261 tcttcttatc tgcccaagtt tctacttgtt gtgccaatgg atgttttgat tgattagtag
    13321 ctcctgattt attcgtgaat ttttgcgtct ccggaaatcc tttttccttc cacaataaat
    13381 atttgtgttg gtaaatttca aaaatatcca agtatgattc gattaatgga tcaagagtga
    13441 tggtgtacaa atcagacaaa ttcattattt ctaagatacg agctttttca gcacttactt
    13501 tttcatcaat aatcgcttta cgttgcgctt tagtcgtcat ttttgtatac accccccttt
    13561 gttttttgaa aaatttgacc taacgatgcg cgtgactccc cgctacccta tctccccacg
    13621 cgaaaaaatt ttgaaaatga ataggggggc ttcaattgaa atacgaagga aaaacttttt
    13681 tgtcttccgt ttcgttttca acaattggat gacattttga acataatagc atgagattgt
    13741 ttggatcaag cttaagcagt tcgttgtctt tgattggtac aatgtgatgg acgtgtgctc
    13801 tctttccaaa tatgaactga ccacaccgct gacaatgacc accttctctt tcataaataa
    13861 attgacgcat atctttccat gcttgcgttc gatagaatgg tttgttctca tgatgataaa
    13921 cagactttgc ttgctgcttc ttcttgcgtg atctgcttga tctcttgtgt tcagtacagt
    13981 agataccctt tgctattttg ttcgtgcatc cgtcaaactg acagtatttc attctacttc
    14041 acgaataaga tttatgatat cgccttttgc acggatagca ccaggaatat caataccatg
    14101 tttcttagca tatgcacgca attcttttgc agtcatattg tccagttcat ccgtatcttc
    14161 agtggactga tcattagtag cttcgtctcc atcaaaatta gcagcagtgt ttccatcgct
    14221 atcaagaatt tcactgctat gagctatgag ttcgccattg acagcaacaa atgatttgcc
    14281 atcactcaaa attccttttt cttcttttac ttcaaaattt ggttcttgat ctttcggaac
    14341 aactactgtc cttttctttt ctgaatccca atactctgtt ccactaatgg atgttctaat
    14401 tttgatcatt gccattttaa ttctctcctt tgtaatttgt acggattact tttgcgccca
    14461 ttcgctcata ccattcaatc gtttcctgca gattgggtag agtgtgtgac aataaagaaa
    14521 taactaacgt attcattcca gttctgtaat catcattctc cacttgcaca tgtgcatgat
    14581 taccattcca attttctctg tacggactat tctctgcttc aattgctttc ttataaactt
    14641 tagccaaact tgattctact tcaatactaa gtaccgcttc atgaaaatca ttcatttgtt
    14701 tactctccta tcagatattt tgtggtatga tttataaaaa agatgcgagg tgttttaatg
    14761 cttcaagaag ttaatattaa gtatcgtgat cgtcaaaata cagagacaat ttatttgcca
    14821 aatagttgtc cgcattgcgg aaatataatg actccgtatg tgtattttgg ggtttcatca
    14881 tatagtgcgt tcgataatga tagaatcttt ggcgtattag tacagtgtat aaatgatgag
    14941 tgcaacaaat tttatagttt acaatttata aatacgttta atgaagatga aggatattct
    15001 gaaaatattt taattaaaga ttattaccgt ccgccgatta aaatagattt accagaaaat
    15061 attgaaaaag tttctcctac atttgttgat atttatactc aagccacaca agctaaatct
    15121 gagcaattgg aacaaattgc tggagttggt tatagaaaag ctctagaatt tctaattaaa
    15181 gattatgcaa tatctaaaaa ttcagaggat gaagataaaa taaaaaaaat gttattaggt
    15241 aaaacaattg aaaattattt gaatgatttt cctaaactcc aaagattagc caaagctgct
    15301 acttggattg gtaatgatga aacgcactat gttcgtagac atactaataa agatgttcat
    15361 gatatggatg catttattaa atcagcagcg caatttatcg cggctgatta tgatgctgat
    15421 ttagctgatc aatttataaa tggaaataac tttatgccaa ctactaatta aaagaataaa
    15481 agagccacag ataccaatta ataaaacggt tgggatatct tggctctttc atattttttt
    15541 gacactaata gaatatcatg ctgctaacaa tgtttacata gctacaatga ccgaacattt
    15601 agtgaacagt gtaattttag aataaagaga gttgttcttt gtacttctcg ctattgtaaa
    15661 aatcaatttc gttttctttt ttcaatcggt cagcttgtct gtgttcatac tcatctaaaa
    15721 agttcaatgt ctttctaatt tctgcatgtc gctgtctgac atacgatgag ctatacccaa
    15781 cagattcagc aatatcttct aagctcattt gatcaacata tttcatctta acaatttgat
    15841 tatcgatacc agaaaagcta tctatcagca ataacatttc ttctttttgc tcgatcaaca
    15901 attccaattc attttctatc ttttggatat tctcttctaa agaagaagtt ctcgaattct
    15961 tttcgatgcg gacgtttgct aaatcaccac agacccatcg attcaattca agcttacttt
    16021 tattaagatt ccactttaag taaagaatct gctcatctag ttcttggtaa tcttttaacc
    16081 aatgaaatct cacaaacgcc accccttatg gtagaatagt attgcgctgc tatctcgaaa
    16141 ggggtagctt ttttattttt tcttagccgt tccattctgg attgcctttt gttccaaacg
    16201 tcgttttttc ttcttaatct ttgattttgt ttttcccata caattctcct ttccgaaaac
    16261 acattttgtt cgatttttaa tactaaataa aagttggcca gaatttttcg ttcgtcttta
    16321 ctcatctttt tttgaataag attactagca taaaaatagt tagtaccacg aaaatgaaca
    16381 ggtaaatcaa ttacgttcac cttcttcatg ccagttatca gccactagaa tctttgcgtt
    16441 agtgccgcca ccgttttctt ttttgtattt ttcaaatgct ttataagcag ctgtttttga
    16501 tttatagatt tttccggaac cgactgggaa catccatgtg ccctctgtga tagctaagat
    16561 gtatactttc atttaattca cccctttaat ccccaacact acatatccgg acttctgcat
    16621 gtaatcagta atataagtga tctctgcttt gtatgagtca ccagtgtaac taaatgttgg
    16681 tctcgttagt ccaggtaatt catgtttaat gtatttttta tattcacgta gtattaactg
    16741 atcaccaact ttgaagtcac gatcattctt gcggatctcg aattgtttgc gtccgctcgt
    16801 cactgcttca aaatattctg gtagtatttt tagttcgtgg atcattctgt ttcctccttc
    16861 aaaattcgct catcatcaaa aagtgaatct ccacaaaatc cttctcccaa atatttatcg
    16921 ttcatgtata agtcaaccaa atatttgtga tctgcttctc gatattcatt aatattaacg
    16981 atctttattt tgtaaacatt accttcgtcg ctcactatct tgaattgttc tctgactttc
    17041 accaccgttc ctcctctttt tgaaaaaatt ttagtgtttt tgtaagaccc cctaaatcag
    17101 cttttttatt tgatataata cttgtatcga ttaatcaaat cgaaattaaa tttaaaaagg
    17161 tgatttatca tgaatatcaa caatttagta aaactcgttg aacaacgttt taaagaatca
    17221 ggattagaac aattaaaaaa agataattct ccagaattat tttctaattt taccgaagac
    17281 gacattccta ctcttaaaca attaaaatat tattctgatt attggacgca aactcgtgag
    17341 aatgaattaa atttagaaaa actcattatt aaaaagaaat agcaactcca gcccacacgg
    17401 ctggtttttt agtttcaact tccctcctta cgggtcaaaa tcatcggttc cggcataatc
    17461 tgatcatcct ttagcaacaa atcaggatat tttttcctta gcttcttatt gttcgtgtaa
    17521 gttttcaata gccattggtt ggcttcgctt ttgctttctc cagaaaaata aattttccct
    17581 gtttggatgc ctgcaagttg aatcatcatt ttcacctcaa atcaaatttt ttctgctaga
    17641 tggagcaaaa gcaatccgac atcaagaatc ttttgaccca ttatttcagc tactttgctc
    17701 ggcttaactc cttgagaaaa gagcaagcgt gcttgctcta tctcttcgtg agtccaaata
    17761 aatttggctt cctcaaggat aattactgga tcagttgctc tcattgttcc tctggaaaaa
    17821 tgatgtcatt cttatgcttg gaccaggtat ctgcatatgg aacaaagtat tgtctagcta
    17881 attcgatttg atcaattaaa gcagtttctg ataattcatg gtctgcagca atttcattca
    17941 tactatcgcc ttcatctaaa cagataagac cgttgacgtt atcgattttt acatttgtga
    18001 ctgcttgact ttctccacgc tcataatatt ctacagttga tactgataca tcagcttcgg
    18061 agtttaattg cagaatctgt tactgccggg ataatttaat attcttttca taattcacct
    18121 tttttcctac tgacatcgaa tatttttcat gttatgatta gccgtgaaag gagagctata
    18181 ttatgtgttt acacatgatg tccttagctc attcggttct ttggaagctt ttttggaaat
    18241 aactctcagg ttcttgacgc aaatctcatt tattctttga aacaaaaaac ttccattttc
    18301 cgagagcttt ggatcacttc aaaagagatt tgcatttata tttctgtgac cttgagtcgc
    18361 gtaaagaacc ttacagcaat ggcggcaatt ccactttcag gaaaccgcct tttttcattg
    18421 atattggtgg ttagcggact acccattttc tttcggcagt gccaaaccac aaatgatctg
    18481 tgttgatgtt taggtagttg tataccagat aagctaaatc caaagtgcct gcatcttcaa
    18541 gacgacaata ttccaaaaat tctcccatag ctggacataa aaatcctgga gctatcatat
    18601 cccaacagtt aatagtgcaa agaaattcgt ttagcttctg aacgttttct ttggtttcac
    18661 gaattacaac atactccaat tcttctccgc tggcagagca ttccaaaact tcaaattgat
    18721 taagaaatgg tagaacatat gcttcaaaca tcaacattcc atctttctgt ttttgcactt
    18781 gttttgcatc aatcatgtct atcctcctat tcagttattt cttccattaa tggaattatt
    18841 tcaggtcact ggacttaacg aaaacaccat tgaccatctt tccagtccga cctttgattt
    18901 cgtcataagc aaaatccaag cactcgtata aatccatatc attttgcatt gccaagatga
    18961 tcaaggttac tactacatcg ccaattccgt ctcttaaacc atgttcatct tttctagcta
    19021 gagaagcggc aacttccccg atttcttcga ccgtttttaa catttgcttg ctgaaatccg
    19081 cttgatccaa tcccttatct tttgcccact gctctacttt cgcgattagt tcgtccatta
    19141 tttctcctcc acatacctaa actgtcttcc ttttgaatca atccataagc tcctagctct
    19201 atcccaaata atatttttgc tcagaccagt aatttcagat aactgtttag cggtacccgt
    19261 tactagaatt cgatcaccat gccagattgc aatctttctc ggcgttctcc gtttgggctt
    19321 ttcagtccac attgatttac cgagcttttg gacttctgca actatttttt tatcttcctg
    19381 ccaatcttct gaataagtca attcgataat tcgctgcatt gccgctttct tatccatccc
    19441 gacattctcc tttcagtaat ttgagtactt gatcaagtgc gctctcacgt ccgccatgaa
    19501 atgtgttgag ccacttgtct tcgtacgaca cactttgtct taaagcttct tgatgcatta
    19561 attcgatctg tgctgtaaat gtctttaggt ccatcttgtt tacacctgct caagttcatt
    19621 aagatgtttt tgcaagcctt taacacaatc aatgaatagt aatttggtat atgctaaatt
    19681 tcttaattgc gttccatcga tatagagtgc gaaatagtat ctgagtttac tccaactcga
    19741 acgatcattc ttaattcctt caattccagc ttcttcgagt tgatcatata cgtctctcag
    19801 gacgtctaat tcctcgccct ttttataatt cgctatttca ttaattagtt ctagataatc
    19861 gatcttcatt tttccacctc ttagaatggt gcttttgatt gtctattagc tcgttctagc
    19921 gcttttttct tgagataggc ttcttggtcg attgcccatt caggaagctt ctctcgtctt
    19981 cctgtgcgct tgtatccact gcttgcgttc ttaggttcac ttttttcttt ccttgcccaa
    20041 cttcgaatag ttgccaaata gtttttataa gtcttaccag atgattcaca atactctgac
    20101 agtcgttcga ttcgctcttg gtaatcatta gggaattctg ttttgagttt ctccatctgc
    20161 tcatctgaca aaagaacatt tttatactct ccgtatttat gacggacggg cttagccttc
    20221 gattttttcg aaggcggtaa ctctcttata tattcttttg tattattaaa tgtattatta
    20281 atagatgtat tattatcttt gactttttcg tcaatagggg tattgcgttt ttcgtcaata
    20341 gggtattgat taattcgtag gtacctattg attatttgat tggtaccctc tttgtaaatg
    20401 atttcccgat tcaagtatcc aaacttaatc aaatcactta cccatcgcga tatggtctct
    20461 ttattcacac catataaatc tgcaaagtac tcattgcctg cccaacaaaa gcctctttca
    20521 ttacacaagg ccgttatctc tccgtataac aacttagtat ttggtttaag tcttttgtcg
    20581 taccttacgt tggctggtat aatcgcataa taacttcgat gttctgtcat ttttcccctc
    20641 caatatttaa ctttttgatt gttttctggt ttaatttgat ccctttgatt tgatatttat
    20701 ttttgaaatt aatcacacct attttgtgct tctctgtgtg atggattctg cagagtgctg
    20761 caaatgtgta ctctgcatga tcaacttctt tgcgctttcg tcttcctagc gctttgtcaa
    20821 agtgatcgat gtcagctcct gttttgccac agatgcagca gactcttttt gtgatgcatt
    20881 tgtagaagta atattcttga ttcgcaggta aaatctcata gccttctttg aaaggaatat
    20941 gatgttcaaa gatgaaatct aagatgatat ttgctaagac attggcatca ctcacagttg
    21001 tattcgattc gtctttcagg cttattttgc gccctgtgac accttcaaaa cgaaagtaga
    21061 agaattcctt ccagaagtcc gttggcatgc ctgtatcgat aaaaatatcg cctatcagcg
    21121 catagatgaa gtttcgttgc tgtacggtga aacgtctagg atcaataaaa cgaatttcaa
    21181 tgactcgatc accatcgtag ccgtcataca tcgtctttag tcgatcaatg ttcacttcct
    21241 cattgatggt tgcgcttatg tcttttcctt tgaacttttt cagaaccgct gaatatgaat
    21301 cgattaatgg tttaaacact catatcactt cttatctaat tcttttctct tagctgctat
    21361 tgctcgctcc atcaaggcac attgctcata gcttaactgt tcaatagttt caacgttatc
    21421 agctaagagc cctaatttat ctgtctgctc attaacatat tcgattaagg ttttggtcat
    21481 atctttaccc atctgctcat tgaaagcttc tagaatcgtc tctagcatgt ttaatttctt
    21541 tgtatcgatt ctaggtggtg ttggaatatc ttccccttga aatacatata atcccagtcc
    21601 gtgtagagcc aatgctttca caaagcatcg cttcaatgag ttattgattt gcattgcatt
    21661 tggtttaaca actggttggt ttcgataatc taaaacagga aataattcgg tttccgtgtg
    21721 tcctttaacc gttactgaga cagatacata agtcccagtt tcatccataa gaaaaggttt
    21781 atattcctca acaagaaagt cttgatgagt tccagaaaca accctgtagt gtttgtactc
    21841 attaatagtt accgttgcct gtggatcatt ctttttcata atctcccacg cgtgagccca
    21901 agataaataa tcaaaatttc cttttttctt gagaatttta tttaacttac gactaaaaag
    21961 tttttcaaag ttcgttgtcc ctttgatttc actcatcaaa ttctgcctcc atttcagcaa
    22021 tgtatttctt acctggtccg taataagaga tatcaatcaa gttatctctg tcgtactctt
    22081 ctagcgcatc aatcaagcca tcttcgatga cgtaaatata ttcaggttta ttcgaatgct
    22141 tcgatagatg gataagataa acatgatccc aaatagttac atggttgcct aaatcatctt
    22201 gatcccaagc tagttcttca ttcgtcaaaa gatttcgtct gatttttcga ccacttgttt
    22261 cctcaatttt cggcttgccc caatcaggat caatcaaata ttgatctaga gtggaaagtt
    22321 ctttttccat atgctaaaat ctccttatga tgtgttttct ttgtgactct ttgcttgccg
    22381 gcggagtcac ttttttattt gttgccatgc tttttgcttg tcaatatgtt gttggcttag
    22441 gatgattggt ttatagtatt tccaccagca attagcaatt gccgtcccta ttcttagcgc
    22501 ttcagctcta ttcattgtca tcaccaaaaa gtctctgttg tctgttcagt tgatcaattt
    22561 ccatacggat cgcagtttct ggcaaccaca tttcaataaa tgaaacagca tcatcgaatc
    22621 tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat tcagaccaaa
    22681 attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt ccccctagaa
    22741 ctcccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt ccgctaattc
    22801 gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct tctaattgat
    22861 agatcatcat atcttcaatt gtttgaggaa cagtattctt gcgaataaca tcttccattt
    22921 cgttgaatgc ttcaatgtat ttttgtttga agtagatagc tttctttcct gtaaacccca
    22981 tagccagcaa gaaaaatcca tctctactaa tgaagaaaac tcgtcgattt ctgccatatg
    23041 aatctggttc attaccttct acaaacatct gtccaaaatt ggacacatct ttttttagtg
    23101 catcaatatc tcttaaaaca tgttgatgtt ttttctcgaa gctttctgcc acttgtaagc
    23161 tcgtagtcac agcttcttta tttttcaaaa ttactaattc ttgcattatt tcttctctcc
    23221 tttttggtat aatttagtta aaaactggtg gtgtttattt tgaatttttt tcacttaaca
    23281 atttttcaat ggatgtcaat aataagtttt atattgagta cgtttttaac agggataaaa
    23341 ataaaaggaa tgcgccctca actagaagtt gaacttaatg cttcttactt cgctgcagat
    23401 atgatttata caaaagtaat catttctaac tattcaacag aaccagctat gttagttaat
    23461 ttagaacttt catccgctaa cttaaaccgc agatggtcag ctactccatt taagaaatta
    23521 attgctaaag gaggatcagt cgatgataat agaatatact ctgaagctgt accgctaaat
    23581 attcctccta aaagtgctat atcttgttac cttgcatttg aagtagggaa aattaatttt
    23641 aataaactcc ttaatcacca aacaaaaatg atatttactc taaatagaac ccaaatccac
    23701 aaaattgttg atatcaaaaa tacaaacttc ccaatagaga aattagtgaa agaattaaac
    23761 tgagtaatag taatatacat tctagtttct cttttctatt catttttagt cagccccctc
    23821 ggttggcttt ttcgctctgt actcagcttc agcttcatca agccccataa aaatccaaac
    23881 catgtaaaca atcgttccta tcaacgcttg cctgcttccc caaagtccta aagcgtagat
    23941 gattagcggt gcgctgaaca ctagcgctct attaaatttt cccatccgct tgcctcctta
    24001 agatttccga aaaatttgtt tctaaaaatt caagtgtttt acttcttaaa aatagatatg
    24061 tgtctcttcc ttcaactgga taatagacaa atccattttt gtttttctcg atatcgataa
    24121 tatttctata tcttgggttc tttaaaactc tagaagtaaa ccaatcatat tttctgttaa
    24181 tccgttctag cacttctggc aacgtcatcc atctaccagt atcatcagct ttttttaact
    24241 cctcataatc cacttgggag ataattacat agccttctgg aattgggatt tttgcttcta
    24301 gatattgcat tagctgttcc tccctttaca attcgtacat agtgataatc gaatctataa
    24361 ttctgtttgc ttctgcagaa gtctttttac cgtttaaaat taaagataag tagcttttgc
    24421 taattccaaa tctttcagca agcatggtgt aagttaagaa ctttgaactt tcgacatatt
    24481 ctttgatttt ttctctatct cgttgagtga tttctgcaat gtcagtcata ctaaaactcc
    24541 tttctaacca atttcctcta aatccatttg agggtaatat ccttcttttt ttagtaattc
    24601 gtagataaat agacgccctt tctgtgtcca tttggtattc attacaattt tagtgccacc
    24661 atcggctttc gggatctcag ttgtatgaga ttttgtgtat ccttggttca tgtgtttttt
    24721 gcacaataac cattggttac cgactttttt ctgaatacct agtttatgaa gtaatttatt
    24781 catctgttgt ggagacatcc cataatctgc tgcaatctga ctaattgtta ctgaatctgt
    24841 agaagataat atgctatcta aataggagat tttaggttcg tactctgcaa tcttttgttc
    24901 tgcgattaat cttccagttc tttcttcttt tagttgagtt gctaattgaa tgattgtatc
    24961 tggattaagc aaagcttctt ctactttttc tggagttaga taacctccat gttttctaat
    25021 tgctggcaac acttcacttg ttacccaacg tttaaatttt ttggcagagg gaagttttga
    25081 ttttaagatt aaactgtaca aaccagattc gttgatgatt gtcatctctc ttgattgacc
    25141 tgaggtcgtg attcgcgacc ccatcttatc ttccaaatct acatgccgcg acaaagcatc
    25201 tttagtgttt gaataaccca aaacactcgc aacgtctttg cctacaaaat atggttcatc
    25261 atttactaaa attgtccgaa cttcgttttg ttcgaaatta aaaatttgcg gtgtgttcat
    25321 atttctcatt cctttctttt atattcgtaa acaaatttaa caactttttt ctaaattcaa
    25381 ttgacagtat ctgagtaata ctctatacta agagcataat taaataagac ataaaaacat
    25441 tgattttaaa gctttcttgg cggtctgcat ttacatatca atagtgtttt ttgttgtctt
    25501 tttagttgtt aaatctgttt acaagatata gtatagctca ttactcagaa ttagtcaaat
    25561 tatttctgcg tatttttcta aacttttttt gtaaacattt agaaaggttg ttttatcgat
    25621 gaatacctat gaaataataa aagacttggc gaagaggaaa aagatatcta tacggcagtt
    25681 agaaattaat tttggatatt ctaacggtta tctagcaaag tggaaaaata atactcctaa
    25741 ttcagatgaa ttaagtcgga ttgctgacta tttcgatgtc tctgtagact accttctagg
    25801 tagagaagaa agagaaaccc ctaaacatgt ggatttatca gaagacgata ctgttttttc
    25861 ttttgatgga aaagaaatat ctaaggagac aatgcgtaaa gcgattgcaa ttgctaaagc
    25921 tttagaggaa aatgaatagt tggagtgatg gtttgtatgt atttgaaatt gaaagaaatg
    25981 ttgagcgaat acaatttgaa agtagtttat atggagatga aagagcctgg tttctactat
    26041 ccaaaaccca gaatcatttt tttgaatgaa aatctatacg gtgaaactgc agaagccttt
    26101 catttatctc atgaacttgc acatttcagc gcctctcatt ttgaattttc agttttgtac
    26161 gatacttcta caacttttca ctcaaaattt gaaacagaag cagataaaat agctattcta
    26221 attttattaa atatctatat tgagaacgaa ctgactgatg aatctcagtt taatctcgaa
    26281 aaatttatgg agtattattc tattcagaat aagctcagat acacttgtta tgctgtctgc
    26341 caatgctatt ttaagaaaaa atattcttac gcaaggcaat atgtatgaat acatcaagaa
    26401 ttattaaaaa aaccgatttc agcaaaacat taaaaaagcc cgtgctgcaa cacgggctct
    26461 ttcctcatat atgagcttct acaaaaaaaa tcatatcaca gaaatgagga agaaaaaatg
    26521 gaaaaagaga ttttaataaa atctccagga aaaaccttga ttaaagtaac tgatgattct
    26581 atttcaatta ttagaaaagg at
//
LOCUS       DASNNU010000032        25701 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.032, whole genome shotgun sequence.
ACCESSION   DASNNU010000032 DASNNU010000000
VERSION     DASNNU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.032"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            133..3660
                     /locus_tag="U8116_001285"
     CDS             133..3660
                     /locus_tag="U8116_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEN8434292.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="U8116_001286"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="U8116_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEN8434293.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="U8116_001287"
     CDS             7448..7789
                     /locus_tag="U8116_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8434294.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="U8116_001288"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="U8116_001288"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEN8434295.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="U8116_001289"
     CDS             9146..11566
                     /locus_tag="U8116_001289"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEN8434296.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U8116_001290"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U8116_001290"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEN8434297.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="U8116_001291"
     CDS             13000..13737
                     /locus_tag="U8116_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN8434298.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="U8116_001292"
     CDS             13751..14566
                     /locus_tag="U8116_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN8434299.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="U8116_001293"
     CDS             14579..15226
                     /locus_tag="U8116_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN8434300.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="U8116_001294"
     CDS             15242..15898
                     /locus_tag="U8116_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN8434301.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="U8116_001295"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="U8116_001295"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEN8434302.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="U8116_001296"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="U8116_001296"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEN8434303.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="U8116_001297"
     CDS             18240..18758
                     /locus_tag="U8116_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN8434304.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="U8116_001298"
     CDS             18865..19365
                     /locus_tag="U8116_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN8434305.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="U8116_001299"
     CDS             complement(19645..20565)
                     /locus_tag="U8116_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEN8434306.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="U8116_001300"
     CDS             20730..21743
                     /locus_tag="U8116_001300"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEN8434307.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="U8116_001301"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="U8116_001301"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEN8434308.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="U8116_001302"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="U8116_001302"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN8434309.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="U8116_001303"
     CDS             complement(23383..24144)
                     /locus_tag="U8116_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN8434310.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="U8116_001304"
     CDS             24333..25427
                     /locus_tag="U8116_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN8434311.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..>25701)
                     /locus_tag="U8116_001305"
     CDS             complement(25513..>25701)
                     /locus_tag="U8116_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN8434312.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         8509 a         4455 c         5417 g         7320 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actggaaata aatgtctgat tcacttttct aaaatccatt gatttcggca gtccattacg
    25681 acgcagaatc ccgttagaat t
//
LOCUS       DASNNU010000033        25287 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.033, whole genome shotgun sequence.
ACCESSION   DASNNU010000033 DASNNU010000000
VERSION     DASNNU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.033"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            235..1875
                     /locus_tag="U8116_001306"
     CDS             235..1875
                     /locus_tag="U8116_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEN8434313.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2029..3351
                     /locus_tag="U8116_001307"
     CDS             2029..3351
                     /locus_tag="U8116_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEN8434314.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3413..3844)
                     /locus_tag="U8116_001308"
     CDS             complement(3413..3844)
                     /locus_tag="U8116_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8434315.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3908..4744)
                     /locus_tag="U8116_001309"
     CDS             complement(3908..4744)
                     /locus_tag="U8116_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEN8434316.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4934..5236)
                     /locus_tag="U8116_001310"
     CDS             complement(4934..5236)
                     /locus_tag="U8116_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEN8434317.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5262..5693)
                     /gene="ruvX"
                     /locus_tag="U8116_001311"
     CDS             complement(5262..5693)
                     /gene="ruvX"
                     /locus_tag="U8116_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEN8434318.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5690..5956)
                     /locus_tag="U8116_001312"
     CDS             complement(5690..5956)
                     /locus_tag="U8116_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEN8434319.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6210..6935)
                     /locus_tag="U8116_001313"
     CDS             complement(6210..6935)
                     /locus_tag="U8116_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN8434320.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7301..7371)
                     /locus_tag="U8116_001314"
     tRNA            complement(7301..7371)
                     /locus_tag="U8116_001314"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7337..7339),aa:Gly,seq:ccc)
     gene            7488..8240
                     /locus_tag="U8116_001315"
     CDS             7488..8240
                     /locus_tag="U8116_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN8434321.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8256..8735
                     /locus_tag="U8116_001316"
     CDS             8256..8735
                     /locus_tag="U8116_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN8434322.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8986..10566)
                     /locus_tag="U8116_001317"
     CDS             complement(8986..10566)
                     /locus_tag="U8116_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEN8434323.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10559..11302)
                     /locus_tag="U8116_001318"
     CDS             complement(10559..11302)
                     /locus_tag="U8116_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8434324.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11478..12254)
                     /locus_tag="U8116_001319"
     CDS             complement(11478..12254)
                     /locus_tag="U8116_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN8434325.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12502..13338)
                     /locus_tag="U8116_001320"
     CDS             complement(12502..13338)
                     /locus_tag="U8116_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEN8434326.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13349..14278)
                     /locus_tag="U8116_001321"
     CDS             complement(13349..14278)
                     /locus_tag="U8116_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEN8434327.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14470..14823
                     /locus_tag="U8116_001322"
     CDS             14470..14823
                     /locus_tag="U8116_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN8434328.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14897..15553)
                     /locus_tag="U8116_001323"
     CDS             complement(14897..15553)
                     /locus_tag="U8116_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN8434329.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15779..17713
                     /locus_tag="U8116_001324"
     CDS             15779..17713
                     /locus_tag="U8116_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN8434330.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17799..19022)
                     /locus_tag="U8116_001325"
     CDS             complement(17799..19022)
                     /locus_tag="U8116_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN8434331.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19016..20914)
                     /locus_tag="U8116_001326"
     CDS             complement(19016..20914)
                     /locus_tag="U8116_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEN8434332.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20908..22026)
                     /locus_tag="U8116_001327"
     CDS             complement(20908..22026)
                     /locus_tag="U8116_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN8434333.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22109..22942)
                     /locus_tag="U8116_001328"
     CDS             complement(22109..22942)
                     /locus_tag="U8116_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN8434334.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22935..23852)
                     /locus_tag="U8116_001329"
     CDS             complement(22935..23852)
                     /locus_tag="U8116_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN8434335.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24079..25203)
                     /locus_tag="U8116_001330"
     CDS             complement(24079..25203)
                     /locus_tag="U8116_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN8434336.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         7798 a         5044 c         4304 g         8141 t
ORIGIN      
        1 ctatttatct atctaacata tcctttattc ttctactttt ctttctttag catataagag
       61 atacgcatag taatcaagca aggattgttg atataaaaag aacaaatacc gctattttga
      121 tttcttttga ctctattact catataaata aaatttatta tacctgctct ttttgactat
      181 atcctcatct gactcctata cttcgtttgt attaaaactt aggaggaatc attcatgctt
      241 acagggctaa acttattaaa taatcctttt ctaaataaag gaacagcttt tacaaaagaa
      301 gaacgggcaa aatacggaat cactggtatg cttccaagta ctgtccaaac attggaacaa
      361 caatctgtcc aagcttacgg tcaatactta agcaaacaga ccgatttgga aaaacgtatc
      421 ttcttaatga atctattcaa tacgaaccgg acattgtttt ataaattgat gggcgaacat
      481 ttagttgaat tcatgcctgt cgtttatgat cctgtcgttg cggattcaat cgaacaatac
      541 aacgaaattt tcttgaaacc acaagatgct gtatttcttt caatcgacga accagaaaat
      601 atcaaagcaa gtttgaaaaa cgcagcagat ggacgcgaca ttcgtttgat cgttgtgaca
      661 gatgcagaag gaatcttagg tatgggtgac tggggtgtga acggtgttga catcgctatc
      721 ggtaaattga tggtctacac tgccgcggct ggtatcaatc ctgcacaagt cttgcctgta
      781 tcaatcgacg ctggtactaa taacgaagaa ttattgaata atccattata cttagggaat
      841 cgtcatgctc gtgtagaagg cgaaacttac tacgaattca ttgatcaatt cgttcaatca
      901 gctactgaat tattcccaga attgttatta cattgggaag attttggacg aggaaatgct
      961 gcaacgatct tagaaaaata cgaagataaa atcactactt tcaatgatga tatccaaggc
     1021 acaggaatcg ttgtactagc tggtgtatta ggtggattga atatatcagg tgagcaattg
     1081 aaagatcaaa cgatcttgac atttggtgct ggtactgctg gtgtaggtat cgcaaacatc
     1141 cttttagatg aaatgatccg tcaaggtgta cctgaagaag aagcacgcca acatttctat
     1201 caagtagata aacagggttt attgtttgaa gatacaaaag gattgactcc tggtcaaatc
     1261 ccattcgctc gtaaacgctc agaattcact aatgctgatg aattgacaaa cttagaagca
     1321 gttgtaaaag caatccatcc aacgatcatg atcggtactt ctacccaacc tggagcattc
     1381 actgaagaaa tcgtgaaaga aatggcggct cataccccac gtccaatcat catgcctttg
     1441 tctaatccaa caaaattggc agaagcaaaa gcaaaagatt tgattgaatg gacggacgga
     1501 aaagctttag ttggaacagg tatccctgct gctgatgtgg aatataacgg tgtaacttac
     1561 caaatcggtc aagcaaataa tgcgttgatg tacccaggtt tagggcttgg tttgattgct
     1621 tcaacagcta cacgagtaaa tgctgaaatc atttctcaag ctagtcgagc attaggcgga
     1681 atcgttgatg taacaaaacc aggagcagct atcttgcctc cagtagcaaa aatcactgaa
     1741 ttctctcaaa cgattgctga aactgtcgca aaatcagttg ttgcacaaaa attgaatcgt
     1801 gaagaaatca cggatatcaa agaagctgtt gaatctgcta aatgggttcc tgaatataaa
     1861 tctttagaag attaagattg ttatagtaaa agaacataca gaaactttgt atgacgtcta
     1921 aaaagaaaaa agcgactgcg gcaaaacagc agccagccaa ctactgctct tttgccgctt
     1981 ctctttggct tttttcatac gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa
     2041 acaacaaccg caactgcagt tgaggaaaag aaaaaagaag ggttttggtc aacaaaaatt
     2101 accggcgtaa gtttgcccct ttatttgatc atggtccttg tcctgatcat tgcaatggct
     2161 ttaggaaaat taccgacgaa tatgattggc ccaatcgctg ttttggtcat tttcggtaat
     2221 ttatttcatt ttatcggaac aaaaatccct atagtcaaaa gttatttagg tggtggctcc
     2281 gtttttgcga tctttgcctc tgccgcgatt gctacatttg ggatcttacc tgagtatgta
     2341 gtaaaatcca cagaaaactt tgtcagcaat atgggctttt tagactttta tattgctgcc
     2401 ttgattactg gtagcatcct tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt
     2461 tttattcccg ttgcacttat ttcaatggtc gtctcctttt ttgccgtagg actcgtaggt
     2521 atgcttatcg gaaatggttt tgcaaattcc gttttatata tctcattgcc cgcaatggct
     2581 ggtggtgttg gagccggagc tgtaccttta tcaacgattt atgcaaatgt tttaggtacg
     2641 gacgcttctt ctatcatttc ccgcctgatt cctgcgacag caatgacaaa tgtcctagct
     2701 atcattggtg cagcgttagt tgctcgtgcg ggacaatcaa tgccaaaatt caacggtaat
     2761 ggtaaattaa tggaaaaagg cgaccttggc gatggaaaac ctagaaatgt aaaaccttcc
     2821 attccacaat taggtgtcgg tttgatggta tcattgactt tcttcatctt aggacaaatc
     2881 tgtaactatt ttgttcctaa agtccatgct tacgcattca tgatcattat cgttgttatc
     2941 tgtaaaatcg caaacctatt accagaatat tacgaagatg cagctatcat gttcaacaac
     3001 ttgattgtca aaaacttgac agcagctgtt cttgccggta tcggtattgc gttattgaat
     3061 ctaaatgtat tggcttctgc tttgacatgg caatttgttg tcttatgttt gacaagtgtt
     3121 atcgcgattt cagtagtttc aggatttgtc ggtcgtttgt ttggtttata tccaatcgaa
     3181 tcaactatta ctgctggctt atgcaacaac tcgatgggtg gtacaggaaa tgtagctgtc
     3241 ttatctgcag ctaatcggat ggaacttatt gcttttgctc aaatgggcaa tcggcttggt
     3301 ggtgccatcg ttttgatcat ctcaggtttc ttgatgcaat tactttctta gtcaaaaaaa
     3361 atacctgatc atcaagtatc ggcttgatga ccaggtattt tttgataaaa atttacagga
     3421 ttttcaaaaa tcttttttgc attttatcgg aatgataacc gatgcgttca taaaatcggt
     3481 gtgcttctac tctgtattct gcagaattaa gtcttattgc tgatattcct cgtcttttcg
     3541 cttcttctac gccagtcatc aaggcctttc cgattcctct tttttcagca tgtttagaca
     3601 cagcaagtgc cataatattg aacatcggat cgctgaaaat cgattcatag gactccgctt
     3661 gtacatagcc agctgctttt ttagaagtag gatcttcata gaccaagaaa aaattttttt
     3721 ctggctcctt caacagtttc ttcatttgtc tcaccgtaac ggttaatggt acatcatatc
     3781 ctaattgttc cgcattgatt tctcgtagtg cttctgcatc tcctaaacgc atttcccgca
     3841 acatccaaat caccagccaa tttttatttt tttaaatgaa tttttttatc acagtatact
     3901 ccttttttca cttaaaaaaa gacttttcag tgattttgac ttgcttgaat ccagatcctt
     3961 ctaatgtttg caagatgacg aaagcattgt aaagattgat caaaagaacc acgatgacaa
     4021 tcgcttgaaa aataaaggtc actttttttg gagacaaaac cctgctaaat cttgctcccc
     4081 acaaacctcc aataacagca gcggggataa cataaaacaa catagataaa tcaaagcgca
     4141 taaagctact tgttaaagca atcgttacca attttgccag ttgagaaaag aaaatcgtag
     4201 atagagaata tagtgttgct tcttttattg gcaatgcaaa catcagcatc aacaaagaaa
     4261 cattgactgg tccaccacca atacctaaaa aactagctaa aaagcccaga acccccccac
     4321 atataaggta ccaagtccat gacgtcaaat gaaattttgg ctgatgatgc ttggtgtaga
     4381 agaacgcaaa aaccaaggtc aataccgtca aaaaaatttg gatcagctgc acatgcttct
     4441 cgttttcaaa taactgtaaa aaaacttcga atacaatgtt gccgactact cctccaacaa
     4501 cagctccact agatacccaa ccgacaatct gccaattcag tgatttcccg cttgatactt
     4561 gtcgcacagt cgaaacaatg gacatcgtga ataccgcaac tgttgagtaa aaagaaatcc
     4621 ctgcaacaga atgggcgccg ataagatcaa gaatcggttt gatcaacacg cctccaccca
     4681 tcccggaaat agcgccaact gtgttcgcca gaacgatcac cacaaaataa atcagaccaa
     4741 tcatcttgta tttcctttct cttgtttata tcttttagcg attgttactc ttttctttta
     4801 ttatacagta aaagaaacaa acgaggcttc gaaagctttt ctacttttta aggaaattta
     4861 gtgctaaacg aaaaaaccgg gctctacttt tcagagagtc cagcttttct tacaccttat
     4921 cgattctttt gttctattct tcttccgcca tgaatgtatt gaatacttct tcgatcatat
     4981 cccattctgc gtctgtttcg atttgttcta actcgccttc tgttccatct tcgttttcga
     5041 tgtatgcata agcttgtaat tctacatctt catcttctga aacaccagct ggatataaaa
     5101 gaacatagtt acgtccaaat tcttcttgtc cgtcaactgt tagcaagatt tcgtataatg
     5161 tttcatttcc ttggtcatcc accaatgtga tgtgttcgtg tccttcgtgt tcgtgggaat
     5221 ggttatgttc tgtcataaga ttacctcttt tctttttatt attaaaataa ttgcttacct
     5281 gagccgtcta aataattttg taaaatcatc acagctgcta atttatcgat gacttttttt
     5341 cgtttcgctc ttgaagtatt tgcctgctct actagcatac gttctgcctg aacagtggtt
     5401 agtcgttcat cttgataatc aaccggcaag gagaatagtt ctttgatctt ctctccatat
     5461 gacatggaag cttctgccct tggtccgatg gaattgttca tattcttagg taatccaact
     5521 acaaaacggt ctacttcgta ctgcgccact aattccttca accgatcaaa accaaactct
     5581 tcttctgcct cattgattcg aatgatttct actccttgag ctgtccagcc aaagggatcg
     5641 ctcacagcaa cgcctactgt cttagaaccg acatccaagc ccattactct cataaattca
     5701 ctccatgatt tgagaggtag tctttgacaa ttacttccat gatttcatcg cgttcgtgac
     5761 ggcgaatcaa atttcgtgca tcacgataac gaggaatgta ggccgggtct ccagaaagca
     5821 agtagccgac aatctgattg attggattgt agcctttttc ttccaaagcc cgataaacaa
     5881 tcgctaatgt ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa cgtacggttt
     5941 catctgtaaa acccatgatt ctacacctct ttcccgcaag gtaattccag cttaccttct
     6001 acacccttat tttactcgaa gaaagggaaa actacaaaca aatattccaa acttctgaat
     6061 aaattaagaa acacgatttg tctgattttt ccttttttct gtggcttttt caaaagccct
     6121 ctttccataa agtatacgct atatttcttt gaaagtatgt ttttacacaa ttttttcata
     6181 aagttcataa aaaattcgaa ttgctgctac taatttttcg ttttcgttgc ttgccctgtc
     6241 tcataattga tggtgtagcc atcttgaaca ttgtagataa agacattgaa ttcaattttt
     6301 ttgtcttcaa tgctttctgc ttccaactgc acacctcttg ctaccagttc atttccctcg
     6361 aaatgaggag tgacacgata gcgtacatga tgatttgttt ccttgatata tgccgcaata
     6421 tcattttcgt gggcaagcat gtacggagca ttcaacgaac gtgtccctgt cattaagttt
     6481 ttaatgttat tattttgacc agtcaactgg aaaccaatca aatggctgcg gttgtataac
     6541 cattgtccgt cacttaattt cttttgtttc caacctgtcg gatcaatata caaaggttct
     6601 ctcttttcag taggaaacaa gtcttttccc aacatcgcat tggcttgtcc tactctgttc
     6661 aatttgtcta tatttgaata gctttcccat gtccctttgg aaagactaag atcctctttt
     6721 gtaaactccg gctgattccc attgatttct atttcttgat gttttccatc atattctggc
     6781 acatccgcta cttgagaagc ttgcggtgtg gaatcaaaaa tggttgtaat ttgttggaag
     6841 atatctgaat ctttttgtgt ggtggaaata ccgagtgccc ctgcaatcac agcagcgacg
     6901 atccccacga tcgccttggt tgtttttttg ctcatttctt tactccttta ctaacagttc
     6961 cctaaatctt aacatatgtt cccctataaa taaatactgg aaagaaatag aaacagtaaa
     7021 aaaataaaac cagtttttag acggcttaag aatacaaaaa aagagacgga ttcatcgtct
     7081 ctactcaatc acaaaacact atttacaagg tatttattat cattcaaaaa atttttctta
     7141 atactcaaaa ctaaacggct tctgtacgac ctcttggtaa acagtgttca aaagcttgca
     7201 tctgcggtaa taagtccaac caagtcaaaa acataagaag cttttttgct tgactattct
     7261 tattacttga tgcagggcag ctttttcaca gcctctttta agcgggtgac gggaatcgaa
     7321 cccgcgacat cagcttggga agctgggatt ttaccactaa actacacccg cgatacaaaa
     7381 acagtatacg cctctttcac tccttcctca agttttttta ttctttacgc ttccgatatg
     7441 ttttgtagta aaataaaact aatcaatcaa ttattggaga agatgctatg acgggaaaag
     7501 aattagatct gttgctggac acttgtttac tcgctgggaa aatcatgatg gaaagcaatg
     7561 cggagatgta tcgggtagaa gatacgatga gtcgaattgc tttagcttct ggaaatttcc
     7621 gtttggtcag ctatgtcact caaaccggtc tctttattgg tcttgatcgt acctcgacca
     7681 tccgaatgga acaaattacc aaccgttcga ttaatttaga aaaagtagta aaagtaaatg
     7741 atctttcacg taaatatgtt gcttcagagc taacattaga agaactttac tgcgaactgc
     7801 aagcaatcga ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag
     7861 tgatcagtgg aacaattatg gtgttatttg gcggaaagat gaccgattta cctgtgactt
     7921 tagtcattgg aggattggga tacgcagctt atctatatag tttgaaattt tttagaatca
     7981 aatttttatc ggaattttta tcttctttat tgattggcat agccgctatc tacagcgtac
     8041 gttttggctt aggaagcaat caggatctaa tcattatcgg atgtgtgatg cctttagttc
     8101 ctggagttca aattacgaac gctatccgtg atttgctagc tggacattat gtctcaggag
     8161 tttctcgcgg aacagaagcg atgatgactg ctacgatgat cggttttgcg attgctttta
     8221 ttttccagct tttttattag gaaaggaaca caagtatggg gacgatattt attcagtttt
     8281 cttttagttt tttagcgact gcggcatttg ccgttattac aaatgtacct aggcgttcat
     8341 tgatttgttg tggtctttct ggtgcttttg gctggatgat ctactggatc tgtgttcaaa
     8401 taggcggatc tgctgctttt ggttcattgc ttggttcttt aggagtagca ttcatcagcg
     8461 atcttttctc taaacgtttg aaaatgcccg taacgatttt caatattccc gggatggttc
     8521 ctctagtccc tggtggttta gcttatcaag cggttcgaaa tctcgttact gaatcctatc
     8581 gagaagcggc tcattatgcg gtccaagcaa ttatgattgc tggagcaatc gctttaggtc
     8641 ttgtattatc agaagtcttt aaccataaca tccgtaattt caaagtaaag cgtgacgcaa
     8701 tcatgatgaa acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg
     8761 tgaaagagac gttcagcctt gagcgatgac aaaatatctc acaaacattg cgacaagtaa
     8821 ccgcaggagc aataagaagt aattatgagt attaaaaaaa gttttgtaaa aatagcttct
     8881 cacattttta cataaaattg acttaatact gagaaactga cttttgaaca ccctctatct
     8941 gtaaaaaagg ggctgcgaca tgatttttgt cacggcccct cttctttatt caaatttttt
     9001 ccaaaacgtt cgttgataat aaacgatcca tagagcggaa agtattagaa ctaaaccgat
     9061 cacaggaata ttcactaacc aaaatggata aaacaaagcc aacattccgt ataagacgat
     9121 aagaataata ctgatgatca atgaacccat catagttgcg actaaaccca aacttcctga
     9181 acctctgtta aataattggt taatattggt ccagttcagc agcagcaacc gataatctct
     9241 agcgaaataa cgtagtgaca ataagtagca gccgaataaa gaaccaaata gcaagctagg
     9301 gataaaaaac aaaggcagct gaaaagacag tccgcttaat aatgcgatcc caccagttaa
     9361 aaaactctgc aatatccatc cgaatctgaa cttttctttt aaatattggt tcatagagat
     9421 tggcaaagaa cggataaaca gaaaattttc ttgatccaaa gaaataagat tgctgataaa
     9481 agaagtctga ttaactgtta agaaagccaa tgcgatccct accaggaaaa caacaccgat
     9541 taacttatta tctaaatcag ttaattttat ttcgcctcct agggcaaaag caagaataaa
     9601 aatgattgga gttaacaagg aagaagaaag aacttgcata atcaaattag gttcttttac
     9661 taattgagca ttgtaactaa ataaaagctg atgcacattt tggttctttt tatgggttct
     9721 ttttattttg aactcactgt ttgaatcagt cagttgctct gtaagctttg gtactatcag
     9781 ctgttttaca gcaaggaaaa atcccagagc cgtgatagct agtattcccc aacccaataa
     9841 accttgttga gaaagtggct gactggcagc atcatagaat ggcaataata tcgaaatcgg
     9901 atgccgatct gttaactcat tttcaatagt ccccgtttga tgattcatcc acatgattcc
     9961 agcaaatgcg atcagcatcg agctgccaag caagatacta gaaactgttt ttctatgttt
    10021 tttaaacact gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat
    10081 aatcaaaaat agagaaaaca aaaggattga gataatgata gtaagcaaaa tccctagatc
    10141 agctcgccat ccggttaaaa taaacatgac tagaagcgga aaaacaaaag gaatgatggt
    10201 caaagcaact accaaaatct ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa
    10261 aaatacttgc aggtcatttc cttcaaaaaa gatattataa atgactgata tactttgagt
    10321 taaacctaac ataccaaaaa gtgccacgta ataagtaaag aaacctggca atttaacaaa
    10381 gttcatagca aacatagcaa atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt
    10441 ttgagacaaa agatagatag ttaatcgctt tccctttttt cctttctctc ttgctttatt
    10501 cgtgagctgt ggattaacat ataaaagatt aactcgaatt aattctaata attgtcgctt
    10561 atacatacgg tccatctcct gtcgcttgcc ttccagccat tttcaaataa atcttttcta
    10621 gcgtttcatc aggtgagcca ttcaataatt cactaactgc cccctgataa atcaattcac
    10681 ctttcttcaa aatagccagt ttatcgcata actgttgtgc ggtatccaat acatgtgtag
    10741 aaaaaatgac tgtttttcct tttgcggcat gagccttcat catttctttc aaatcaaatg
    10801 ctgcttgagg atcaagtcct tgcaaaggtt catccaatac ccaaatatct ggatctggca
    10861 gcaacgtgcc gatcaaaatc gttttttgac gcattccatg tgaaaaactt gcgattgttt
    10921 cattttgatg actatacata tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt
    10981 tttgtgtgtc taattcatag gcagctgaaa tcaagtccca atattctcca gccgtcaatt
    11041 gtaaaaaaat atctggggaa tcaggcacat aaccgatttt ttgtttgata ctcaatcgat
    11101 tttccgtcaa tttcattcca tctactaaaa tcgttcccga tgatggttca ataatactaa
    11161 ctaaactttt aatcgtagtt gattttccgg caccattatg tcctaaaaat ccaaaaattt
    11221 ccccttgtct tatcgagaga gacaagtctt ttaatgcttg ttttttcccg taacttttac
    11281 ttacattgat gaattcaatc atttctgtac tccttttgtt tgttattact ttttattgta
    11341 cccttcgtta ttggtggagt aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg
    11401 actaggctgc tacttgggtt gttctcttct acaggaaaac actcttctaa caggtaagat
    11461 atgagtcttt gttttcttta aaatgattca ttttctatct tttcttgcat aaaattcttt
    11521 agtttatcaa tacttctctt tcccatctct agttgttctc catttgcaaa tgttactatt
    11581 tcgtttactc tgctaataga tgtaatgttt tcagtattaa tgatataagc ttttaaatct
    11641 ttacataaat actcatattc gagtgatttt ttatattttt taattgtgcc gtttacgaga
    11701 atctcttcat ctttcaatac taggcctatt ttatttttat aacctggcat gctcattaga
    11761 taaaggattt catcgtaatg gatatagtga tcgctattac cataacggac agttgtccac
    11821 ctttttttcg tagcatgttg aacaggatag ttttcttttt ctttcaataa gtaactttgg
    11881 atgctttttt tatttttttc ataagtgaat tcttttatca aataaccact aggcgtaatc
    11941 tttcgattaa taacagttat tgctttattc ggatgagacg ttagataaac aattttacac
    12001 ttttcattct tttctctaag catttcgcct agttcgatac cgctataata ggcgttcaaa
    12061 tcgatgtcta ggaaaaaaat atctgaggaa tagatttcat tatttttaat gaattcaaca
    12121 aattgtataa cgttcaatcc ctgaacgatc tcaaatttcc aaggacttaa taaaattctg
    12181 gattcttcca aaatttctac tagcattttt tcaaaaacca aactatcatc caaaataaat
    12241 atacgcgtta ccataaaaag tacctccact cgatatttta ctatcaaaat catttcatac
    12301 aacttttcaa catacatgtt aggaataaat tgtaattctc ttcacaatct tttccaacac
    12361 ctttgaaacc gatatcaaat actttcagga tgctgcttct aaaataaata tcaaacaaat
    12421 gaatacatca tgcaaaaaaa gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct
    12481 atgtttattt attttgttct tttaaaaaga acgatacaca tactgatttt tcggagcaga
    12541 gactttctca gcgtttgtaa caactacaga tgggatttca cgcttgaatg gttgataata
    12601 gtctgattgg atggttcctt ctattttata ccattcatca tttttaaccg ttactccttc
    12661 cggcatatgc gtcaacaaac caaagactcc tgaatctgcc acacaatgga taatgccgaa
    12721 tcggaacaag aagatatctg tattgctatc gttcggtgtt tggtaaacaa atccttcata
    12781 gctgattttt ttgccaatga attcgctagg gtaattataa attagctcca taacttctaa
    12841 atagttttca tctgtgatct tgatagtttg cttatcccca tacttttctt tcaactcatt
    12901 catttggtct aagtagtctg atttgttgaa ataaatactg gtatccggct ttaagtattg
    12961 tgtctgcatc tccggatccc ctactgattc tttacttact gggaaattaa atcctttggc
    13021 ttctacgatc gtagtgtcga gactaactgt tggaaacaac gtccccacga ttactggcaa
    13081 agctagcagt acataagcga ttccacgttg ataaggtttt gacaacccat ggtcatgatc
    13141 gtgctcgtgt gccttttctt ctgcttttcc gtttttgacc cacaaataca gctgaactaa
    13201 agccaaaaga gcagataaaa tcattgaaag aatagctaag tagcgataat ggacattgat
    13261 atattgatcg agtttgccag aaacttgcaa gtacatcatc aattcgaaat acccagataa
    13321 aatcaaaaac cgcagcatat tttccctcct aaataaataa tgtgaatcca ataacgctaa
    13381 gggcaatcag tccgatcagc gataaaataa agcgtcctgt aaaatatcgt ttcatcatca
    13441 gtagattttt gatatcgacc attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc
    13501 cgaacagact taacaaagaa gatcctataa acgcatctgc ttctgagcaa agagacatcg
    13561 taaaggccaa taacagcatg atgataatgg ctaaaatctt tgttgagccg agcgttagca
    13621 taattctagt tggtaaataa gtctgcatcg cagctgcgat taatccgcca atgatcaaat
    13681 aacgacctgt atcaaaaaat tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga
    13741 tcagctgatt ttttggttca ttcttctcac tttgttcttc ttcatggaca tgatgatggt
    13801 gattttctgc tactttagac gtgatttttt ctttcaatac agcgtctttt tgaaaatagg
    13861 caagccagac accaatcaca agggcaacaa aaaagctgcc tatcacccgc cagcttgcca
    13921 tctgccaaga attgccaaat gcgataaaag tggaaaaaat cacgattgga ttgatgatcg
    13981 gagcagtcag cataaaagca aacgctgtgt gaactggtac gcctttttta acaaattgat
    14041 gaacgatcgg tacgatgccg cattcacatg aaggaaagaa aaatcctaag atcgaaccag
    14101 taaaaataga gaagatacga ttttcaggaa gccatctctt cacacgttct ggggtcaaaa
    14161 atacttgtaa ggcgcctgag ataagacagc ctaataatac gaaaggtaat gcttcaataa
    14221 cgatcgataa gaaaatcgta ctcatctgca aaaccgaatt tggaataaaa gaaaacatga
    14281 gtttcctcct ttttatttct agttcattcg ctttgcaaat atacgctttt attcaaaaaa
    14341 aacaagttgc tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga
    14401 ctttttctgt tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg
    14461 agaataaaaa tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca
    14521 tccgttgccg tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca
    14581 cgactagagg aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa
    14641 acaccagaag tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat
    14701 caaggaaaag aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta
    14761 ctagatgaac tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa
    14821 taatcgttgt ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt
    14881 caatcgcttt ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca
    14941 agaaatagat caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg
    15001 acggagcatt caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta
    15061 actgattgac tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt
    15121 gttggatctt gatttgttct gacatatctt ccattggata aacggggata ccgtctacga
    15181 tgcgcccaac gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt
    15241 gatggaattt gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat
    15301 ttgtcaattg atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat
    15361 aaccataccc acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac
    15421 tatctacttg aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat
    15481 ttaaaatcct taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa
    15541 tatgatcttt cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc
    15601 atagcagtat ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct
    15661 ttattcattt atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta
    15721 ctaattgaaa aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat
    15781 gatattatta caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa
    15841 catacattta gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg
    15901 aaaatcgact ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc
    15961 taaaaataaa acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga
    16021 aacagtttgg aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat
    16081 gcgcgagcta gagattttca ttagcgaagg tacgccagac acacctgctt atagttctta
    16141 tttaaaagaa tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga
    16201 aaatgaaatt cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa
    16261 aatcgatact ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca
    16321 acaccctgac attttgattc tagacgaacc aacgaaccat ttagatatcg agacactttc
    16381 ctggcttgag aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg
    16441 ttatttttta gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca
    16501 ttacaaaggt aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg
    16561 gaaggcttat gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa
    16621 ccttgttcgc gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat
    16681 ggaccggctg gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga
    16741 aaagccttct ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt
    16801 caccttatct gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg
    16861 tccgaatggt atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat
    16921 ccgtggaaaa gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc
    16981 tgatctgcat acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc
    17041 agaaaaagag atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa
    17101 accaatccct ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat
    17161 gaacaaagaa aattttttga ttttggatga accaacaaac catttagata ttgacaacaa
    17221 agaagtatta gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga
    17281 ccgatatttc atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa
    17341 agtctatcta ggagactatg attattattt agagaaaaag caggaagaag aagaaatcgc
    17401 cgaattgttg gcacaagatg agccagaatc tgctcctgct cccacgaaca aatctgatta
    17461 ttaccaaagc aaagaacagc aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt
    17521 tgaagaagaa atggcgcgga tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc
    17581 gccagaaaac ctggaagatc acgtaaaact caatgagctg aaccaagatt tagaagctgc
    17641 tagacaagat caagaaacaa aattgacgga atgggaagaa ctaagcttgg aattagaaga
    17701 atatgaaaat taacgaaaaa accaaaacag aaaccattca tgctgttttg gtttttcttt
    17761 ttttatttta tcaaacaaaa attccatatt tacctttttc accaataaga agaccaatta
    17821 ctagtcattc tcactaaagc ttctaaataa tagtaatcac cccaaataca aggtgtattt
    17881 accccattat ttttgggctt attataaaca gactgtgtta aaaagccatc ttcttcttcc
    17941 gttgtagcat atctttcttt caattgtcgc ataattttcc aaacagcaga ctggtataag
    18001 ttcttttctg ttttgtctaa tagacctgat tctattaatt ctaataatcc acaaaccatg
    18061 atcaccatac tagatgtatc tttttcttct ctgctagaat catcaaagat caaatcccaa
    18121 taacaaatgg tatcttctgg caaccgattc ataaaataat tggctaatat tttgcttaaa
    18181 tcaaaatatt tttgttcttt caaataacga taacttaatg caaaaccgta aatcccccat
    18241 gcttggcctc tagcccaaca ggaattatca ctataacctt gatgcgtact tccatatctt
    18301 ggttttccat ctttgacatc catataaaat gtatgataag ttgaaccatc ttctcttact
    18361 aaataatctg ctgcattttg aatatgagcc tgagcagctt ttttatattt ttgatttcca
    18421 gtttcttcac tcgcccaata caataatgga agattcatgc aacaatccat gatcattcta
    18481 ccttgttgtg aaggatcact aggatctccc caagcttgta taactcctgc cttctcccaa
    18541 aaacgctgaa gtaatgtttc tgcagcatca attgaagctt ccttcatttt tcgatttccc
    18601 gttattttat aatctgctac ggttgataaa gaatacaaaa aaccaagatc gtgatgatta
    18661 gtgataattt gacagtcaat cctgtggata aaatctatat ctgctttttc agctgtttgt
    18721 tgaaataaag gatcacccgt cttctcaaat aacaaccaat taattcctgt ccaaaatcct
    18781 tctgtccaac caatattgtc tcttaatgga taacgtcctt ttataggaca ctcgcttggg
    18841 aattgatact gactgttact gatacgtttg attaacctct ccgtgtgtct gcgacaacgc
    18901 tcgatctcat tattccattc tttttccatt aattctttat aacattcatt tatccatttt
    18961 tctggatgta ttatctcttc aaacatcact ttatcaagca tttccccacc agcctttagc
    19021 ataattttag tcttttttta ttgtttttat tttggatagt tagcccggtt gtcccatcaa
    19081 aatcaatttt gggacgattg ttccatactt ctaaactata atcccgatct tgatgagcaa
    19141 ccactcctgt tccgagccaa tgggttcctt ttccaaatgt accctctaat ccaggaataa
    19201 aagtgttttc ccatgtcatc aagtttgtat tgggcaaacc cataatagct gtggattttc
    19261 gtttaatagt atcgtcactt aaacagataa tacctgaaaa gccagcttca ctcaccctat
    19321 agttttcctt gtcttcttct atgattattc cttctttctc aggagaacat tcaatagcaa
    19381 accctgtctc atatagaaca tattctgtat ctaattctac tttgtgtacg cgaatctgcc
    19441 agccttcaaa aggaataagc caagttctga tttttgcctg taaagtaggt tcccatgtag
    19501 aggcaagtac ctctttctta catacaatgt ctcgtacatt atttcgaacg atgaactgtc
    19561 cataggtttt gatgctctca ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa
    19621 tccctagcat tgaatccatt gccatttctg ctttcaattg attcgctcgc ggtatcgaaa
    19681 aaccaaattg actagaataa gcaaatttta aatatttttc attggtcaat gtatggaaat
    19741 ttggccctaa ttgtcccgca ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca
    19801 aattggcttc tgaaagaagt ttagttcctt cacgaatagg cataggcatt tctttagccg
    19861 tccaatactc atggtcttca ggtagcgcca acaataaaaa aatcttattc agccataatg
    19921 gagataacgt actattatag ggttcagtca atgtcaattg agggtaggta taaccaatat
    19981 tcaggatacc tttttcatca aatatttctt tttgcatcca ccatcttata ttccgattga
    20041 ctaacccctt gatttcacct aatttaaaag gcaaaagatc ggcccacaac atagctgacc
    20101 aaaagcttga aactgcaaaa cgataaatca tgcttcgacc aaaaggcaca tttgcaccat
    20161 cttccgcaaa aaaatgaata tagtctctag aaaaatcaat agctcggttc ttatatatct
    20221 ggctaatggg cttttctggt tctagtacgc tataaatcaa accataataa tgaaatgcaa
    20281 aaggattata ataatcttgg cgacctcttg aactgtctcg ataccagcca ttttgaagat
    20341 aacaattttc tatattatca aatgcttctt tcagtgctga ttctttaact ggataaccaa
    20401 atttttggat aattgttccg acaattatct taaaaaattg ccaattacca tctgcacatt
    20461 catattcaaa aatttgcgag aaccaatgaa taatctggct tttttctcct gtatctaatt
    20521 ttttccataa taattgttca tgatgatata acgctaataa cattgcgggc atttctacca
    20581 tacgctggtc tctttcatgt atttctcccc aataatcggg atgggaagga tctgttccag
    20641 cttttattcc tgatagaatt aatttcaacc atcgttcatc taattggtca attgcgggcc
    20701 ccgctcccca taaaacccga gagaatcctt ccattcctgc aattttattt ccatatccta
    20761 ccccttgatg tatataatca atttgtgcgc cacttgctcg ataaaacggc tggagtggtt
    20821 gatttatctt tttaaataag tcaaagtaat cctgtctggt ccttaatagt ggtactggat
    20881 gtttcattgt attttctcct ctttttacta ccaaatagct aattccgact gttctaatcg
    20941 aagtaagcct tcaataaaaa aataatcaga atagatcagt ggcacattat aagtcagttc
    21001 tctatggtat gcttctgctc cgcctccaat gatcccatct tcatccatat tccaatttgc
    21061 atagtgcttt tctgtctttt ggagtatatt gatagcagct tcatgataca tttttgatcg
    21121 tacagaggaa acatatcctg ctaattccag aagaccgcat gcagcgcata ctccagcact
    21181 ggtatcaata ttcgaatctt ttacagcaaa atcaatgaga ggaatataac cagttttttc
    21241 gattttctga ataaaattta ctgctatctt ttctgctata gctagatagt acggattttt
    21301 tgtgtaacga taacttaacg cgaatccata taaagcccat gcatttcctc tgctccaaca
    21361 agaattctca ttgtatcctt gcccgccaag taaccgaata tatttccctg attctgcatc
    21421 aaattctgcg atatgcccgg ttgatccatc tttcctcact agatacttag ctgttgtatc
    21481 tgcatgctgc cgagcaattt tagagaacct cggatcacca gtttccctcg aagcccaata
    21541 cagtaatggt aaattcatca tactatcaat aatcatccaa ccaggtttat ggtcattcca
    21601 tgctactaaa tagttccctg aaatattata gcgtccagca agtaccgtag ctgcatgtaa
    21661 ccctagttgt cgactatgtt tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa
    21721 gaataaaaaa cctacatcat gatgaacgtc tgaaaaatgg tacagggcag attctaataa
    21781 ttgttcttgt tgtattgctg cctctttgaa cttttctcgt tttgtcttgt gaaagagctg
    21841 ccataaaata ccactccaaa aaccattggt ccaccagtcg attcctctgt cctttattac
    21901 gtctgtatat tttccgttgt aagaaatata tggaatttta ttccccattc tgtctaattc
    21961 cgcatctatc ttttcagaag ctacattcaa aatttttctt gaccattcct tcgtctgttc
    22021 attcatggat atcccgccat tcttttaatt tttatgaatg tggagttgct cctagaacga
    22081 actagatggt taacattcgt tatttttttt acttatttgc agctccaatg tcattctcct
    22141 taaatttaga ctgcgcccac acataaatca agaccattgg gatcactgaa gttgctaaaa
    22201 ttagcataat tgcgccataa ttaattggct cacctgtact agaaaccgtc atatttttga
    22261 taattaaggg aaccgtatat ttatctttat ctgaaagcat gactaatgga aatagatatt
    22321 gattccaact tcccataaag ataagtaaac cactagtaat aatcgtaggt gataaaatcg
    22381 gtacagcgat tttcgtaaaa atcacccact cattggcacc atcgattctg gcagcctcca
    22441 tcagtgctgt aggaaattga ctgctatatt gtcgcatcat aaacactgaa gttgtactgg
    22501 caatcgaagg gatgatcaca cctgcataag tattcgataa tcctaaacga ctcataatgt
    22561 aaaataaagg aatcaatgtg gtaaaaaaag gcaccatcct agatagcata actgtattga
    22621 acaacagttt attccccata aaacgatact ttgctaatgc ataacccgcc attgtagaac
    22681 tggctgtagc tgaaactgta ctgattacag caataacaat cgaattccaa ataatcaagg
    22741 gataattata tctcgccatg atggcttgcc agttttttaa tagcatatcc ccaaacttga
    22801 aattgattcc gccacttaaa atttcttgat tactttgcgt tgaagctaca atcatatata
    22861 aatatggcat caaaaatatt aaggcaccaa acacaataat gacgtacgta aaaattctgc
    22921 tttgtatctt ttgtttagtc atttttatca cctccgaatt taaattgaat agccgataat
    22981 ataacagata caaacaccat cgtccaggca atagcagagg cttttccaaa atcaaccatt
    23041 tcaaaacttg ttttatataa attgactcct acgaccaaag ctgaattttc tggtccattg
    23101 gaagtcccaa agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa
    23161 actgtggcca ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga
    23221 aatttcgatg ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga
    23281 ccagctaaaa ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt
    23341 gatattcgta tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc
    23401 aaaaatccat tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc
    23461 accgttgatg taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac
    23521 tgtgctgaat tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat
    23581 cctgccatga taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata
    23641 gctagataat tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa
    23701 gcaaacgaga taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg
    23761 accaatccat aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt
    23821 attgtcttgc ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc
    23881 accagttaat tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt
    23941 ttcaaaatca cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc
    24001 tgaataagtg gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc
    24061 atagtatttt tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc
    24121 caatgagtta gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt
    24181 cagcataaat gtaagaaatt gttgtgccac ttgtggattt ttggcatctt tagggacata
    24241 aaaacttgat cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag
    24301 tggagccatg cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata
    24361 ccagccgcca gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc
    24421 gtagaaatgt acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc
    24481 ttctttagta ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc
    24541 tagcatgttg ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc
    24601 agttttttct tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc
    24661 aaaatctaca cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa
    24721 atcactagcc caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt
    24781 ttgatcataa aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa
    24841 agcatcttga aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc
    24901 cggcatatct tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga
    24961 agctacattc acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc
    25021 agcattttca acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc
    25081 tttttttatc attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc
    25141 acttaagctg aataataggg taatgtttag caaagctatc accaatttgt atcttttttt
    25201 catgtctatc ccttctttct tttcaagttc ttatataaaa cgctttcact tatgtatatt
    25261 aacggaatta gtaacacaaa tatttat
//
LOCUS       DASNNU010000034        24943 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.034, whole genome shotgun sequence.
ACCESSION   DASNNU010000034 DASNNU010000000
VERSION     DASNNU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.034"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..497)
                     /locus_tag="U8116_001331"
     CDS             complement(<1..497)
                     /locus_tag="U8116_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HEN8434337.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
     gene            complement(738..3083)
                     /locus_tag="U8116_001332"
     CDS             complement(738..3083)
                     /locus_tag="U8116_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN8434338.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            3362..4264
                     /locus_tag="U8116_001333"
     CDS             3362..4264
                     /locus_tag="U8116_001333"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEN8434339.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            4475..6250
                     /locus_tag="U8116_001334"
     CDS             4475..6250
                     /locus_tag="U8116_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEN8434340.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVDFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(6316..7902)
                     /locus_tag="U8116_001335"
     CDS             complement(6316..7902)
                     /locus_tag="U8116_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEN8434341.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            8153..8377
                     /locus_tag="U8116_001336"
     CDS             8153..8377
                     /locus_tag="U8116_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEN8434342.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            8519..10270
                     /locus_tag="U8116_001337"
     CDS             8519..10270
                     /locus_tag="U8116_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN8434343.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            10270..12054
                     /locus_tag="U8116_001338"
     CDS             10270..12054
                     /locus_tag="U8116_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8434344.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            12389..13241
                     /locus_tag="U8116_001339"
                     /pseudo
     CDS             12389..13241
                     /locus_tag="U8116_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            13338..15224
                     /locus_tag="U8116_001340"
     CDS             13338..15224
                     /locus_tag="U8116_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN8434345.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(15265..16167)
                     /locus_tag="U8116_001341"
     CDS             complement(15265..16167)
                     /locus_tag="U8116_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN8434346.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            16397..17800
                     /locus_tag="U8116_001342"
     CDS             16397..17800
                     /locus_tag="U8116_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN8434347.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17797..19233
                     /locus_tag="U8116_001343"
     CDS             17797..19233
                     /locus_tag="U8116_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN8434348.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            19378..20304
                     /gene="trxB"
                     /locus_tag="U8116_001344"
     CDS             19378..20304
                     /gene="trxB"
                     /locus_tag="U8116_001344"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEN8434349.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            20517..22244
                     /locus_tag="U8116_001345"
     CDS             20517..22244
                     /locus_tag="U8116_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEN8434350.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            22260..22610
                     /locus_tag="U8116_001346"
     CDS             22260..22610
                     /locus_tag="U8116_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN8434351.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22704..23564)
                     /locus_tag="U8116_001347"
     CDS             complement(22704..23564)
                     /locus_tag="U8116_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEN8434352.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23656..24084
                     /locus_tag="U8116_001348"
     CDS             23656..24084
                     /locus_tag="U8116_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN8434353.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            24246..24422
                     /locus_tag="U8116_001349"
     CDS             24246..24422
                     /locus_tag="U8116_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEN8434354.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            24449..24895
                     /locus_tag="U8116_001350"
     CDS             24449..24895
                     /locus_tag="U8116_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEN8434355.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7879 a         4452 c         5292 g         7320 t
ORIGIN      
        1 taagtagcgt ccataaaaat agcgacatac ttatcattta aggctctgga tttaaaggca
       61 tttacttctt cagtcagaac tttagtcatg ttggacatgg tttgtggagt atagtgatga
      121 ccgtacattt tttcgatcaa atcagcaatt tcagacatcg taacaccttt ttcgaataaa
      181 tggataatag tggtttccaa tgtatcgttt gttcttttgt aggctggtaa agtttgttgt
      241 ttaaactcac cattacgatc tctaggtatt tccaatgtta attcaccata ttcggttttg
      301 attgatcgaa agtaagaacc gtttctcgaa ttacctgaat taaaaccagt gcgatcatat
      361 ttttcgtaat ctaaaaaagc cgttaattca gtccgtagga gtgtgtttat cgctttttct
      421 aagtgcgaac ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct
      481 gtagtaaaat cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat
      541 tctacagaag ggacttcctt ttttgtatgg attttttcat ttacacaaaa tattttatac
      601 tctctggttt ttcacatttt tacataaatt tgtcttaata tcgtagatgt aagcttttga
      661 acaccattta ttcggaaaaa gggagctgtg acaagatttt tgtcgcagct ccctttaatt
      721 ctgattaatc tattagatca atcatcttta tcgtctgatt ctttggtctc tggattcgct
      781 ctagcataag ctgaagcggt gttccagtaa tctgtatagt tttcatcagt accgcctacg
      841 ccgaatttaa aggcgctttt cttcggacca ttttttgccc aaagtgactc cactgttttt
      901 cctggcgttt ggatggattt tttattgaca gtaacttttc cagctgtttg tcctgtgaac
      961 ccagatactt ttttcttcat cacatcggat gataagtcaa atttctcatc agtgccgaaa
     1021 atctcagggt ttgcttggta agcttgattg attagattgg caagataatt agccgaacgt
     1081 attcttgctt ggctgtccat tccagtattg tcatcgtgtc ctgcccaact actgatagtg
     1141 atagatggag ttgagacgat cagccatgaa tctcggtatt cattggtaga accagttttt
     1201 ccgacccaat ctgcatttcc tagatttccg tttaagcttg agatgacatc tttgaatggt
     1261 gtagtgattt tttcattgat cacactacgc atcatgtcat tcatgatcga tgccgttgct
     1321 tcggaataaa tacggacaga attactttca tgtttataaa tcacattacc tgcattatct
     1381 gtgatggaat caatgatata gccttcttcg tagactccac catttgctaa ggcctggaat
     1441 ccatttgtct gttgaagcgt cgttacatct actgtcccta aaggagccga ttcaacgccc
     1501 caattatcat ttgctgggta gttcatcttc gccaagtagt gctcgtaagc gtattgtttc
     1561 gatccaccgg aattcaacac atcttgatat aagtgataag ctgggatatt gttggaccat
     1621 tccagtgact cacggacggt ttggaaggtg ttcgaccctt cattggtcgc attgacgatt
     1681 ttctcaccag cattttttcc ttcttgccaa gtcgttgcat aatctgatac acgggattct
     1741 gatccgacaa gtcccatatc gattgcagga ccgtaaacaa gaaccggttt gatggatgaa
     1801 ccagcttgac gttctgtatc aaaggcgtgg ttattttgat tctggctgta attccgacca
     1861 cccacgaagc catagatacg tccggttctg ttatccatca atacatttcc agtctccact
     1921 tggctaccag caccatcatc taacatgtag ccataatttt ctacgccggt ttgtaatgca
     1981 gcatagatat ctttatcgat cgtcgaatgg atcgtatatc ctttgttttg gatcgtttgc
     2041 tgtgcttttt gataataagc ttcactgacc gatgaatctg acatatcagc attatctttt
     2101 tctgccaatt gttgtgcaat aatccttgtt gcttcgttca tcacggtata atacaagaat
     2161 tctctgtcag tctgttccgc gatttgttgt gggagaaagt cttttgtaag atcataggct
     2221 ttcgcttctt cgtattgttc tttcgtaatt tgtccttcac ggtacatact gaataaaaca
     2281 aaatcttttc tggcaagacc ggcagaaaga tcttctttta gcgcgcctgt attcgtgtat
     2341 ggactatacg tgatagggct ttgaggcaaa ccagcgatat aagcagcttg tggcagagtc
     2401 acatcctttg catctacacc aaagattcct ctagcagctt cctggactcc agcaatattt
     2461 tgccccttat tattccgacc aaatggtgag acattcaaat acgtagtaat gatctcattt
     2521 tttgaaaagt gtttttctac ttgtgcagaa agaacgatct cgtttgcttt acgtttgaat
     2581 gttgtttcat cggttaggat ctgttgtttg actaattgct gtgtcaaagt ggatcctcca
     2641 gacgagccga tgccagtcgc ttctgaaact agtgctcgta aaacagcttt agggacataa
     2701 ccttgatgtt tatcgaagta ttcatcttct gtactgataa tcgcggtttt caataaaggc
     2761 gaaatcttat cagatgaaat agtcgtccgc atcagatctg tctggatttt tgatatctct
     2821 gtattatctg cataaacaat ctttgacgtt tccgtaatgt ttcctagttc tgtctgcagt
     2881 tcttttttag tcggcaattg tgtgtcttcg acaagatagg caaagtagcc agcacctatt
     2941 ccgataccta gagctgcgaa caacaaaaga agaacagtaa taaaaacagt caatgattga
     3001 aacactctaa aaataatctt aggaacaaac catttttctt tttttcttct attctgtttt
     3061 tttcgagtat ttttgcgtgg caaaagggtc acctcaacta atggttttat ttcgctaatt
     3121 atagcaaata ataggacaaa aaaacagaca aggctgtttt tgacaggaat ttctttttcg
     3181 aatgagataa aacgtgagga aatgatcttt tttatgaaag agttcttaag cttttcttta
     3241 gggaaagttt cagttactat ggagaacagg gcaaagaaaa acaaaccatt ttctttgtac
     3301 atcatcttat caatatacag gaattgttcg aaacaattgc ctttcaagga ggaatccatc
     3361 catgttatta ggctcacatg tcagcatgag cggaaagaaa atgctgttag gcgcggcaga
     3421 agaagcagcc agttacggat cttcgacctt tatgatctat acaggcgctc ctcaaaatac
     3481 tagaagaaaa gctatcgaag agatgaatat tgatgccgga aaagcattta tggcagaaaa
     3541 cggactaaca aatatcgtcg ttcatgcgcc ttatatcatt aatttgggaa atacgatcaa
     3601 accagaaatg tttccatttg ctgtacaatt tctgagagaa gagattgtcc gtgcggaagc
     3661 tttaggtgct actcaaatca cccttcaccc aggcgctcat gtaggagcag gggcagaagc
     3721 tgggattgct agaattattg aggggttgaa tgaagttttg acaaaagaac agacggcaca
     3781 gatcgctttg gagacgatgg cagggaaagg tacggaaatc ggtcgaacat ttgaggaatt
     3841 agcgcaaatc atcgatggag taaaattgaa tgaaaaatta tccgttactt ttgatacatg
     3901 tcatacgaat gatgccggat ataatataaa agaagatttt tcaggtgtaa tggaagagtt
     3961 tgataaggtc atcggattag atcggcttaa agtactgcat atcaatgatt ctaaaaatcc
     4021 acaaggttct cataaagatc ggcatgccaa tatcggtttt ggaacaattg gatttgacac
     4081 attgaacaaa atcgtccatg atccaatgtt tgacaacatt tcgaaaatat tagaaacccc
     4141 atatgtcggt aaagataaaa agaaagctta cgcaccatat ggaaaagaaa tttccatgtt
     4201 caaagcgcaa gcattcgatc ccgaagtgtt ccctgagtta ttagcagaag aagtcacact
     4261 ttaagttttc tacctgacag caacaaaaaa ggattgggca gtctgccttg tccttttttg
     4321 tttaagaaac aaaggagtgc tttttgcatt cgttttcgcc atgctccttt tgccccctct
     4381 ttttaaattc agacaaacag ggtatactga tagaaaagta aaaaacgaaa agtttcacaa
     4441 acaagtagca aaacgtgatt gaaaggacga aaagatgctg aacattttaa accccttgat
     4501 cgaccaacta aaaaagaaac atacatactt tattcttggc atagccgtat tatttacaat
     4561 tattcggttg tatttatata gtaaagcaat ctatggaaca gatatgtcgg cgacctatga
     4621 tgatcagctg ctgattcact atgctcaaaa tattttggat ggtaaatggc taggggagta
     4681 tacgactaca acactttcca aaggaatctc ctacagctta tttttagtac ttgcacataa
     4741 actgcatctt tcttatgcag ttttgttcgg catgttcagt atttttgcca gtatattggt
     4801 tgcactcgcg tttaaaccga ttgtgaagaa caagtatctt cttttaagta tctattttta
     4861 ttttttgttt tctccgatta gttttacaca tgaatattcc actcgaacat atcgtaatgc
     4921 cctcgtgatt ccggctgttg agatcattat cgcttgttta ttagctattt attaccgcaa
     4981 atacggttct agcagaaaat tgctgccatg gtttttaggg ctttctgtcg cttttccttt
     5041 cttttggttc attcgcgaag attctatctg gcttcttcct tttactgtag tagctttggg
     5101 tatcagtttc gtccagatct tgctagggca agacttttct ttcaaagaag taaagaagtt
     5161 aaaaaaacga ctctttcttc tctcgaaacg ggaatggctg aaactttcat tgtttttact
     5221 tcctatagtt gacttttcca tcgtctatca gtggatcgaa tataaaaatg agacaacata
     5281 cggtattgca accatcaatg accgttccag tggagaattt ggacgtttga tgaaacaact
     5341 gatccgaata gatgatgaaa cgaacttgaa taaagaaaac agtgagatat gggtctcaca
     5401 tgctgcacta aataaagcat tagccgcatc tcctacattc catactgttt ctacccgtat
     5461 tgacgaatta tatacgactc acgcgtggac tcaaggtgga aaagtaaaag aattacccgg
     5521 agatatcatc ttttgggcat tgcgagatgt ggtaagcgaa tctggttatt accaaaacaa
     5581 tgcaaaagaa accaatgatt tttggaaaaa agtcaaccaa gaattagaag aagcctatca
     5641 caagggaacc atcaaaaaga aaaaagagtt ctatctgatg ggatcaggag atggaaagac
     5701 gatgagtgat ctgccaatcg tattcgattt ccttcaatca ggatacgctt atgcatggcg
     5761 atataaagat ttccatcaag gaggaaatta tagtttcgga acgcaagaac aagtcaaaca
     5821 ggctcaagaa ttattaaacc ttccgctaat ggagaattgg atcgatagta atgaaccgag
     5881 aacacctaat ttccaattaa caaaatcagc aaaagtcgcg aatgtcatca taaaaattta
     5941 ccaagttctg gctattcccg tgctgattgc ttcaatagca gggatattac ttatgatact
     6001 aggaaccgta tttgcaaaag ctgatcgtgc ctattatagt tcctctttga ttgtggtcac
     6061 cggattaata ctgacacaat tggtttttct tttcggtgtc tcatggtttt gttcttatgc
     6121 gccagaaaaa aaggactatt ttttaagtgt ctacactggt gcaggcgtgc cattaacgca
     6181 attcatcgta gtaacaagca tgctagtttt cagtaaatta aatctgaaaa aagctttatc
     6241 ttctaaataa aaagaccctt ggtagctaga tttttaacct aactatcaag ggtctttgtg
     6301 tggaaaaatc agtttttatt cgatttcttc taccgtttcc tgcaaaagct gttcatattt
     6361 tttgtttcct ttatataaat caccaatcgt ccatgccaac aggagaagga caagaacaat
     6421 gattacataa gcgatgtgat cagccaatgc tagttgagcg gaagtggctt gctcgccgaa
     6481 gaaactttcg atctgtcctg gtaatcctcg taagttcaag taagttagac taatcacaga
     6541 gaaccagcca agaagtttta cgatgaatga atttttaaat cggctaccca tctccgcttg
     6601 gctgttggtc atcatcagta aaggaatcat cgaaaatggc aacgcaaaag ccaagaacac
     6661 ctgggattca ttcattaagt tattcaaagc ggcatgttct tcgatcgttc cttttgtact
     6721 tgtaaacaaa acacaaatca atacaggaat cacagaaagc aatcgtgtga ccagtcttct
     6781 tagccaaatc gggatacgca tatggatgaa tccttccatg atgacttgac cggtcaacgt
     6841 accagtaata gtagagttct ggccagaagc aagaagtgct acagcaaata aagtggacaa
     6901 gataccagat tttgctacag caatcaaaat accattgctc atagctgatg gatcagataa
     6961 agcttcatac agaccaaaaa aggaaggatc ttggacagaa ccggttttga agacagcgac
     7021 acccatgatc aatagcagtg cattgactag aaaagccatt gacaactgga tattagaatc
     7081 ccaagtagaa aaacgtacag cttgtgctac atcttcttcg ttttggtgat cgatcttccg
     7141 agtttgtgaa actgctgaat gaagatataa gttatgcggc atgactgttg caccaataat
     7201 ccctaaagct ccagtcagcg gagtttcgcc gccgatgacc ggtgagtctg agaatgtatg
     7261 actgttaggg ataaagccag caaataaact gtgccaatct gggttagaca agccaacttg
     7321 atagacaaaa acgaataaaa tgacgaaaat taagcagaca accagtgctt cgatcttacg
     7381 aaacccgact tttgtcagta gtaagagaac gaaaacatct aatacagtaa taaagacggc
     7441 aatcaccaat ggaatatcaa aaagtaagta gagggcgatt gctgcaccaa tgacttctgc
     7501 gatgtctgtt gccataatgg ctaattcagt caagatccat aaaatgacac ccaatgtctt
     7561 acttgtccgt gctcggattg cttgggccaa atccatttgg gtcacgattc ctaattttgc
     7621 tgccatatac tgtaaaagca tagcaattag actagacatc aaaataactg acatcaataa
     7681 atattggaaa ttttgtccac cagtgatcga agtggaccag ttacctggat ccatataacc
     7741 aactgctact aatgcacctg gtccagagta agcaagaagt gttttaaaaa aactttttcc
     7801 ctttggtaca tccactgttc catttacttc agctaaagat aaaccatttg tatgttcaat
     7861 aagtcgatgc ttatgaaatt gtttgttgga ttgattattc aagaaaaaac cacctttcct
     7921 atttatctac gattacattc tacacctcag aaagaaaaaa ttaaagaaaa agattcggta
     7981 tgcctaattt tttttcgtgg tgtgtcttgc tggttaatga aagaaatttt gttcttctct
     8041 aaaaaaacca ctattttttg catgaaacta aagaatacgc ctttcttttt tcaataaact
     8101 ttagtaaaat aaatcattga gagcaattcg gaaatagaaa ggagagattt atatggtatc
     8161 aacaggtatt gtcgtactta ttgctattat tgctttgcta atcggcgcag taggtggctt
     8221 tttcctagcg cgtaaatata tgaaagacta tttagaaaaa aatcctcccg ttaatgagga
     8281 catgttgcga tcaatgatga tgtcaatggg gcaaaaacca tctgagaaga aaattcgcca
     8341 gatgatgcag caaatgaaga accaagggaa aaaataggtt ctgacccaaa gactttttgc
     8401 gggctgattt tttcagtccg ttttttattt cgtaatttgt tagactattc agactattca
     8461 gcaaaaaaga caagcacaga caatcctatg ctcttgataa agacaaagga ggagtaaaat
     8521 gtcgattttt agaaaattag gttggttttt tcgtcaggaa aagaaacatt atttaattgg
     8581 gattttctcc ctattcgcag tggccttggt acagttagtt cctccaaaag tgattgggat
     8641 catcattgat gagatcgccg ataagaattt atcaataaag atcatttcct ggtggatctt
     8701 attattgatc cttgcagctt tcttacaata tctttttcgt tatatctggc ggacgaatat
     8761 ttggggaagt gcagctcggt tggagagaga acttcggcag cagctattca aacattttac
     8821 agaaatggac aatctctttt accaaaaata tcggactgga gatttgatgg ctcatgcaac
     8881 gaatgatttg aatgcaatcc agaatgtagc aggagctgga atcttgacct ttgcagattc
     8941 agtgattacg ggcggaacaa cgatcattgc gatgattttg tttattgatt ggcgcttaac
     9001 actgattgcg ctgattcctc tgcctttgct agccgttact tcacgagtgc tggggtcaaa
     9061 actccacgat gcttttcgtg attcgcaaga agctttttct agtatcaatg ataagacaca
     9121 agaaagtatc actgggatca aagtcattaa aacatttgga caagaaaaag aagatctcgc
     9181 ggattttgaa gaaaagattg aagatgcaat tattaaaaac aaacgagtca attttttaga
     9241 ctccttattt gatccattca ttaccttgat tatcggtctg tcttatgttg caacaattat
     9301 tttaggtgga aagtatgtct tggaaggcac gatctcaatc gggcagatgg tctcatttat
     9361 tagctatatc ggtatgcttg tttggccgat gtttgcgatt ggtcgattgt tcaacgtatt
     9421 ggaaagagga aatgcttctt atgatcgcgt caatgaactt ttgcatgaaa agacgcatat
     9481 cgtggaacaa ctgggtgcag tgcagacccc ggcaaaaggc gagctgaatg tagctatttc
     9541 ctcttttaac tatccgaaag aagaatcacc agcattagaa caaatcaaat ttgcgatcca
     9601 agaaggagaa acattaggta ttgttgggaa aactggtgcg ggaaaaacga caatcatcaa
     9661 attactgatg cgtgaatacg atgattatca agggaaaatc caatttggcg gacgttcgat
     9721 caaagaatat tcattgaatg ctttattagg atcaatcggt tatgtgcctc aggatcactt
     9781 cctgttttcg atgaccattc gagataatat ccgtttttct aatccaaaac tttcagaaga
     9841 agaagtagag catgcggctt ctttagcatt tatcaacaat gaaatcaagg aattgccaaa
     9901 aggttatgat accttagttg gagaacgtgg agtctcttta tctggcggac agaaacagag
     9961 gatttcgatt gctcgagcat tgatcaaaaa tcctgaattg ctgatcttag atgatgcact
    10021 ttctgcagta gatgccaaaa cagaagaagc tatcttgcat aatttaaaag aaataagaaa
    10081 agagaaaaca accatcattg cagctcaccg tttaagcagc gtgatgcatg caaaagaaat
    10141 cattgttgtc gatcaaggga aaatcattga acgaggaacc caccacgagc tgcttgcatt
    10201 aggtggctgg tatgaacgaa tgtgggaaaa acagcaatta gaagcgaaga tagaaggagg
    10261 cgaggcctga tggatgaaaa ttatcaatcg gaatggacaa aatcgattcc acttaaagaa
    10321 cagaagaaga tcatcaaacg actgatgaag ttcgccaaac cttttagaag aacgttcatc
    10381 gttgctatct tatttgcgtt tgccctgtct gtgatcaatg tcctattgcc aaggatcatc
    10441 caaactttta tggatgatca tctggctaaa cagtcagcta cgacacaagt tattttgttt
    10501 tttgctggtg tgtacttgtt tggagtcata gtcaaaagta tcatttggtt tttccaatgg
    10561 tatttgtatt ccatggcttc attgaagacc tatcagtatg ttcgggtaag attatttgaa
    10621 aagctgcata cactaggtat gcgttatttt gaccagacac cggcaggatc gaccgtttct
    10681 cgagtgacga atgacacaga aacgctattt gaattttggt atgtcttttt gatggtattg
    10741 actgggatct ttgcagtcgt ttcttctttc gttgccatgt ttcaaatcaa tggagaaatt
    10801 gccttgtgga ctttggtgtt cttgccgatt ttaggaatcg tcatctggta ttaccagaaa
    10861 ttcagttctc gtatttaccg aaatatgcgg gaaaaattaa gtcagctgaa cacgaaattg
    10921 aatgaatcga tttccggcat gcgtattatc cagcagtttc gccaagaagc tcgtttagct
    10981 aaagaatttg aacaagtgaa caacgagtat ttagacacgc gctatgcgat gatcaaaacg
    11041 aattcgattc tgttaagtcc aatcatcaat tttctctatg cattggcgat tgcgttggcg
    11101 ttgactttgt ttgggatcga tgcgttgcat tctcccgtag aagttggact gatctatgca
    11161 tttacgacct atgtccagga attttttaat ccaatgtctc aaatgatgga ctttttgagc
    11221 acctttacag atgggatggt tgccggtagt cgtattttga aaatcatgga tacagaagaa
    11281 tatgcaccgc aacaaaatcc tggagcagat caagcgatta cttcaggtaa gatcgagttt
    11341 cgcaatgtaa gcttttctta tgatggagag cacaatgtat tgaacaatat ctcttttgtc
    11401 gctaatccgg gtgagaccgt tgcattagtg gggcacaccg gaagcggaaa aagctcgatc
    11461 atcaacgtat tgatgcggtt ttacgaattt tatgaaggcc aaatcttgat tgatgatcat
    11521 gacattcgag aatatccaat ggaagagtta cgtaagaaaa tgggattggt cttgcaagat
    11581 gccttcttgt tttatggaga tattgcagga aatatccgga tgatgaatcc agctatcaca
    11641 gacgaacaga ttcgccatgc agctgagttt gttcaagcaa acaagttcat cgatgaatta
    11701 ccaaaaggat atcactcaaa agtgatcgaa cacggtgcca gctattctag tggacaacga
    11761 cagctgatca cttttgcgcg aacaatcgtg acagatccta aaatactagt attggatgaa
    11821 gcaacagcaa atatcgatac tgaaaccgaa ggattgatcc aagaaggatt ggcaaaaatg
    11881 cgacaaggac ggacaacgat agcgattgcc catcggctgt ccacgattcg ggatgcagaa
    11941 ttgattttag ttttagacaa aggtcatata gtagagcgtg ggaatcatga aacacttcta
    12001 gcgcaaaatg gtttgtatgc agatatgtac aaactgcaaa gcagtggtgt gtaaaaagag
    12061 gttgtgacag tagcatagta gcattttgcc ctaagcgcta gagtgaaaat aaaacagagc
    12121 ttcgcagatt cttcatctgt gagcgttagt ggcgtagtca cccgatttaa aaaagacgac
    12181 gacagctagt caaaagcggc taataaccac taagaaagcg ttgacaaaag cgtttgcttt
    12241 cgataatata aagaaaacga tacaacttgg atagtgattg cctgatgcaa gctaaacctg
    12301 atgagtctcc catggttcca tggacggctt tttgggtttt tctttttgtt ttggatacag
    12361 ccctatatat catggaaagg agcaaaacat gctgattgag aaagtactga ataacaacgt
    12421 cgtaataacc agggatgaaa gaaatcagga aatgatcgtg atgggaaaag gtttggcatt
    12481 caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc gacaaaaaat ttcagttgac
    12541 aaatgacggg ctttcacaac agttccaaga attggcacaa gcgattcctc ttgagtactt
    12601 agaaatttct tgtgaaatca ttgaatatac aaaagaggct ctacaagtcc agttgaatga
    12661 ttctgtgtat attgcattga cggatcattt gtatacggct atcgaacgtc acaaaatggg
    12721 aatatttgta ccaaatgtat tgctgtatga tatcaaacaa ctatttccta aagagtatgc
    12781 catcgggaaa aaaaacagtg aagaaaatct cggataaata ccacatcaag ttatcagaag
    12841 atgaagccgg atttatcgca ttgcatttag tcaatgcaca aggcattcaa agccaacaag
    12901 acaataccta cctgttcact ataacaatac aggatattct aaatatcgtc cgctattact
    12961 ataagattac atttgatgaa gattcagtcc atttttttcg attcgtcaca cacttacgtt
    13021 tttttctgac acgcgtccta gatggagaaa aatcagaagt gggagaagtt tctgaggaat
    13081 tgtttcaggt tattcaaaaa aaatataagg atacgtttgg ttgcgttgag aaaatagcag
    13141 actttttagc cgagacgttc aactaccaga tgtccacgga cgaaaaaatg tatttaacga
    13201 tccatactgc gcgtttagtg catgagaatc gttgtaaata aataaaaaaa ggaggataaa
    13261 ttaacaagaa cgtatgaaat aagggtagtg acattcagtt ggctaaacct tccactaata
    13321 aattggaggg aatagaaatg ggaaaatacc aagaattagc gagaaaaatc gtagaaaatg
    13381 tcggcgggaa ggaaaatatc aatggtttga cacattgtat cacaaggctt cgtttcaaat
    13441 taaaaaacga agaaaaagca aatgatgaga tcttgaaaaa tatggacggt attgtaacgg
    13501 taatgcgagc gggagggcag tatcaagttg tcatcgggaa tcatgtgcca gtcgtgtttg
    13561 aagaggttat aaaagcagga aaccttactt ttgatgaggc tgtttcaaca gaaaaaatga
    13621 gaccgtttga tatgcttatt gatggaatat cgggctgttt ccaaccattc cttgctatcc
    13681 tagctgctga cgggatgatt cgtggactga cagcattcct tgtcttctta ggcgcgtttg
    13741 accgcggttc tggcacattt gttatgtttg ataatatcgg tgattctgta ttccaattta
    13801 tgcctgttat tatcggttta acagctgccc gtaaatttaa agtaaatgaa ttcgtaggga
    13861 tgttgcttgg cgcagcatta atgaatccta gcttgtcgct ggaagctttg agtggtgcag
    13921 cagaagcacc actaacgacc atattttctg gcactatttt tgaagcacct atttatcaaa
    13981 cagtattcgg cattccctgg attgcgcgta actacgcttc ttctgtcatt cccattatct
    14041 ttattgtgtt gttagctagc caaattcaga aaccaattaa aaagttagta cctgagatga
    14101 tcgcgaattt cttcgttcca ttcttcaccg ttcttattac aatgccttta ggttttttac
    14161 tcgttggacc agtattcaca ttcgcaactg atattttaat ggctggattt gaaacacttt
    14221 tagcactttc gccagttatt tatggagcaa tcgtaggctt cttctggcaa attctggtga
    14281 tgtttggtct ccattgggca atcgtaccaa tgggattgat gcaattttct gtgaatggtt
    14341 ggcaaaatat tatgacacct gttgcagtag tgtcttttgg acaaactgca gcacttaccg
    14401 cactctactt taagcttaga aatccaaaag acaaagcaat tgcaatacca gccattgttt
    14461 ctggtattgt ggggattaca gagccagcta tttatgggtt tacattgcca cgtaaaaaaa
    14521 tatttatttt tacctgtgtc ggtggtgcgt tagggggcgc gtatagtggg ttaatgaatt
    14581 taacttcttg gaatcaaggt ggactaggca tctttacaat tcccaactat attagaccag
    14641 atggtgattt aacagatgtg attaatgtat tgattgggat cgccattgcg atggtggtat
    14701 cctttgcgct gacttttttc ttttggaagg aggaagcaga tgaaacagat gatagacaga
    14761 aaagatcagg taaagaaatt gtaaaaacac cgatacaagg acaaatcgct ccactgaatg
    14821 cagcaaagga tgcagctttt gcacaaggaa cactaggtcg aggaatcttg atctaccctg
    14881 aaaaaggaga agtacgtgcc ccttttgacg gaacagtcat gtccctgttt ccgacgaaac
    14941 atgcaatcgg gatggtttct gaagcaggtc tggaattgct gattcatgtc ggattggata
    15001 ctgttcagtt ggaagggaaa tattttgaaa gcttggtgca gcaaggagaa agagtgaaac
    15061 aaggcgatct gttgcttcgc ttcgacaaaa agaaaatcga agaggaagga tacagtttag
    15121 aaacacctgt gattgtcatc aattacatgg attatttgga tattttggaa aatcaagggg
    15181 aaacggtcgg gccagacgat gcacttatca ccgcactgac ctgaacggtt aggtgaaaga
    15241 atggactagg cttaactatt ctttttagtc cacttcttta ttttcaaaat tgaaaggctc
    15301 tttcatgatg gctgagctga tagggcgttt atcaaatttt tgtcctacag aaattagatg
    15361 gactaaattc tgttgataat tttctgcaaa aacacaacta tataacacgt ctagaagata
    15421 acaaatcgat gtattgatcg taaaattacc aattttcgaa tacagtcgtt cgcgtgtcgt
    15481 aagtcgcaaa gcggctgtac tcattgaaga tagcgttgaa tcaccgatac tggtcaaagc
    15541 taaaattggc acatgatttt gtttcaatat ttctgcggta cgaaggatca cattgttttc
    15601 acccgtataa gaaatcagaa tcccacacat atcacttgtg caattgtacg cttcatagaa
    15661 cgattcacca aaagtggtga caatcgtgac atccattttg atgcgcttca ttttcagcat
    15721 gaaatcttga gaaatcaaag tattcgagtt actggcgaaa atcacgattt tttttgcttg
    15781 aagcattagt tgtttggctt tttgcagttg gtcatgatga atgagagaaa gtgtatcttg
    15841 aatcgtcatc tgttctaaag aagccatttt atttgcgatc gtcataatac cgtctgtggt
    15901 ggaaaaagga agattcgcat ctacgtcttg aaaattccct tcaagatagg ctgtttcagc
    15961 taaaaaagca ttacgaaaac tgacccagcc atcaaaaccc attttttttg ctacacggat
    16021 gagtgtggac gggtggacat aacatcgctc agcaatctct tggatggtca tttcgccaag
    16081 tgctggagaa tcgcttaaga gaaactgaat cacttcattt tctgaaggag aaaaggagat
    16141 ttctttgatt ttttcacgta ataacatgta gataccttct tcttgttttt ttacgtaaat
    16201 cataactttt ttgaaaatga tttgtaaacg gatttatgtt atatagctta ataaagacat
    16261 tttttgacaa gatatttaca aaaatccaga agaaaacact aacttttgta aatgttactg
    16321 gaaagaaacg aatttctatt gtttgttttg tttacaaaaa agataataaa agtgtaaaaa
    16381 gtaaaggagt gagaacatgg caaaaggaat caaaatcgct acgattggtg gaggcagcag
    16441 ttacaccccc gagttgatgg aaggatttat caaaagatat gaggaattac cgatacgaga
    16501 aatctggctc gtagatgtag aagaaggaaa agaaaagctt gagatcgtca gtcagatgtc
    16561 tcagcgtatg tgggatgctt ctccgtatga agtcaaaatc tatgcaactc tggatcggga
    16621 agaagcattg aaagatgcgg attttgttac gactcagttt cgtgtcggtc aattagatgc
    16681 acgaatcaaa gacgagcgga tccctagtta ttatgggatg attgggcaag aaacaaatgg
    16741 tgctggcggg atattcaaag cttttcgtac cgttccagtg gttttggcaa tcgttgaaga
    16801 catgaagcgt ttatgtccaa atgcttggct gattaatttc gcgaacccaa gcgggatggt
    16861 gacagaagca gttatcagat atggcaaatg ggataaagtg atcggattat gcaatgtgcc
    16921 agtaatggcg atgatgacag aaccaacaat gcttggcaag aaaaaagaag agctgatcta
    16981 caagtttgcc ggtctaaatc acttccattg gcacaaagta gcagataata aaggaaatga
    17041 ccgcacggca gaattgattg atcgtttata cgatgaagac aatggattgc cgaaaaatat
    17101 ctttgacatc ccgtttttca aagaacaatt aaaacaaatg aacatgatcc cttgtggcta
    17161 ccaccgctat tactatcgtg aagaagaaat gctggaacat gctttagaag aatatcaaac
    17221 gattggtaca agagcgcagc aagtgaaaga aacagaagca gagctgtttg aattatacaa
    17281 agatccagaa ttggatcata aacctgaaca attgcaaaaa cgaggtggtg cttattattc
    17341 agatgctgcc tgtgaaacga ttgcttcgat ctatggaaat aaagaaacac agatcgtggt
    17401 atctacaaag aatgatggag ctgttcctga tctgccggca gattgtgtag tagaagtcac
    17461 tgcctatgtt ggcgcacaag gcgcacgaaa tgtagcgttt ggttcccttc ctgctgctga
    17521 aagaggctgg ttgcaagtaa tgaagaatat ggaattgctg acgatcgaag cagccgtgac
    17581 tggtgattat ggaacagctt tgcaagcgtt tacgatcaat ccgcttatac gatcaggaaa
    17641 gacagcaaaa cggatcatgg atgagttgtt tattgcacat cagcagtatt taccgcaatt
    17701 caaggaaaca atcagccggt taaaagcaga aggaatcgaa gtccaagatg aactggcaag
    17761 agaattatca agcaatacac atgtaatgga ggaagtatga tgggattcag aaaagatttt
    17821 ttatggggcg gagctacagc agccaatcaa tgcgagggcg gttatgatga aggcggacgt
    17881 gggttagcga acgtagatgt tgtgccaatc ggagaagacc gttttcctgt cattactgga
    17941 aaaaagaaaa tgtttgaatt tgatgaaaat catttttatc cagcaaaaat tgcgatcgat
    18001 atgtatcatc attataagga agatattgct ttatttgcag aaatggggtt caagacttat
    18061 cggatgtcca ttgcttggag tcgtattttt ccaatgggcg atgaaaaaga gccaaatgaa
    18121 tctgggttga aattttatga ggatgtcttt aaagaatgtc aaaagtacgg gatcgagccg
    18181 cttgtgacga ttacacattt tgattgtccg atgcatttga tcaaagaata tggtgcgtgg
    18241 cgttccagag aactcgttgg tttttatgaa aatctgtgcc gagtcatctt caatcgctat
    18301 aaaggacttg tcaaatattg gttgacattc aatgagatca atatgatcct tcatgcacca
    18361 ttcatgggcg cagggctgta ttttgaagaa ggagaaaatg aagaacaaat caaatatcaa
    18421 gcggcacatc atgagttggt tgcaagtgcc atcgcaacga agatcgctca tgaagtagat
    18481 ccggagaaca aagtaggctg tatgctggca gcaggagcat attatccata cacttgcaat
    18541 cctgaagatg tttgggcttc tagaaagtca gatcgagaaa attatttctt cattgatgtc
    18601 cagtcacgag gagaatatcc cgcttatgca ttgaaagaaa tggaacgaaa tggcattcag
    18661 atcaaaatgg aacctgggga tgccgcattg ctgaaagaac atactgttga ttttatctcc
    18721 ttttcttatt attcttctcg agtttcgact actgacaacg aactcttgga acagactgca
    18781 ggaaatatct ttgcttctgt caaaaatcca tatctggaag caagtgaatg gggttggcaa
    18841 attgatccat taggcttgag aataacgatg aatgatattt atgatcgtta tcaaaaacca
    18901 ctatttattg tagaaaacgg cttaggtgca gtcgatacgc ccgatgagaa tggttatgtg
    18961 gaagatgatt atcgaatcga ctatctggca gcacatatcc aggcaatgaa agatgcagta
    19021 gaattagatg gagtcgatct attaggctat acgacttggg gatgtattga tttagtatca
    19081 gctggtacag gtgagatgaa gaaaaggtac ggattcattt atgtcgatcg tgacaacgaa
    19141 ggaaatggca cattagcccg ttcgaagaag aaatcattca actggtataa aaaagtaatt
    19201 gattcaaatg gagaaaatct ttcgaacgat tgatcaatga tttataaaag tctattgttt
    19261 ttcagcggat ttttcgagta taataaagct attcgagaaa cgcaagcaga acaatagact
    19321 ttttttcgca ttggctgtaa agtatgctta cttgattaag taaaggagaa tctacatatg
    19381 tatgatgtaa tcgttattgg cgcagggcct gctgggatga ctgctgcttt atatgcttca
    19441 cgttcaaact tatccgtatt gatgatcgaa caaggcgctc ctggcggcca aatgaacaat
    19501 acggcagaaa tcgaaaacta tcctggtttc gattcaatca tgggtcctga tctagcttat
    19561 aagatgtatg aaggtgtttc aagatttggg acagaaaatg cttatggaat cgtgcaagat
    19621 atcaaggatc acggcagcca caaagaagtt atttgtgaag ataaaactta cgaaggaaaa
    19681 acagtcatta tcgcaacagg ctgtatccac cgtaaattag gcgtacctgg cgaggaagaa
    19741 tttgctggac gtggcgtttc ttactgtgca gtttgtgatg gtgcattttt ccgtaacaaa
    19801 cgtctagtag tgatcggagg aggagactca gcagtagaag aagccatcta tttgacacaa
    19861 tttgcttctg aagtagtcat cgtccaccgt agagatgaat tacgtgcaca aaagatcatc
    19921 caagaccgtg catttgcgaa tgaaaaaatt tcctttatgt gggatacagt cgttgacgaa
    19981 atcgttggaa atgaaatggt agtgacaggt gtgaaagcac gtaatgtcaa aactggcaat
    20041 gagtttgatt tggcagcaga cggagtattc atctatgtag gattggatcc attaactgca
    20101 ccatttagag aagctgggtt gacgaacgaa gccggctgga ttccaacaga caacgaaatg
    20161 aagacgaata taccaggcgt ttttgccatc ggagatgttc gtgaaaaaga tttgcgacaa
    20221 atcactactg ctgttggtga tggtgggatt gctggacaac aagtgtataa atacttggaa
    20281 tctttagaag aaacaactgc ataagtttaa aaagagtgaa agaacggttt caattgttct
    20341 tttactcttt tttttgcgtg aaatctactg aatccgattt atgcgaatac ttgtaaccgt
    20401 gcttgataaa agaatctttt cttgatttaa gcgttttttg gtcctcatac tttcatatct
    20461 aataagtgaa tgctatactt attaagaacc atatttcaga aagaggtgtt attttcatgt
    20521 cttgggaaca agtgtatgaa caatgggtac atgaagaaaa tttagaagaa aacttaaaaa
    20581 aacaattaaa tgatctgggt gaagatcctg aaaaacttga agatgctttt tatgcaccgt
    20641 tggaattcgg tactgcaggc atgcggggaa tcttaggacc tgggatcaat cgaatgaaca
    20701 tctatacggt aagacaagca acagaaggac ttgcacgttt catggataca caaggtgaag
    20761 aaacaaaacg ccgaggtgtc gctatcgctt atgactcacg ccatcaatca ccagaatttg
    20821 ccatggaagc agccaaaaca ttagcacatc acaatatccc ttcttatgta ttcgaaagtt
    20881 tacgtccaac accagagctt tcttttgcag tccgtcattt gaagacgttt gcgggaatca
    20941 tggtaactgc atcccacaat ccagcagctt acaacggata taaagtctac ggagaagatg
    21001 ggggacaaat gcctcctgca gacgcagatg cattgacgaa atatgtccgt gaagtcagca
    21061 atccgttaaa agttgaagta ttatctgacg acgaagctga acatagcgga ttgatcacga
    21121 tcatcggaga agaagtggat gccgcttatc tagaagaaat caaagcagta acgattgatc
    21181 atgaattggt ggaaacaatg ggtaaagatt tgaagcttgt gtacacacca cttcacggaa
    21241 caggaaaaat gttgggcgaa cgtgcgttga aacaagctgg attcgaacaa ttcgtactag
    21301 ttccagaaca agctgtcgca gacccggatt tcactacagt caaatcaccg aatccagaag
    21361 aacactctgc tttcgagtat gcgatgcgac ttggcgagaa agaaggcgct gacttactga
    21421 ttgcaacgga tcctgatgca gaccgtttag gcgcagccgt tcgtttgcca aatggctcct
    21481 atgaagtatt gacaggaaac caaatcggag ccctgtttgc tcaatatatt ttggaagcac
    21541 accaatcgcg aggcacttta cctgaaaatg cagcggtatt gaaatcaatc gtttctagcg
    21601 aattgcctac agcaattgcg gaacactatg gggctaccat gttcaatgta ttgacaggat
    21661 tcaaattcat tgctgaaaaa attcaacaat ttgaagaaga tcattcccat acttttatgt
    21721 tcggattcga agaaagttac ggttatttag ttaaaccttt cgttcgcgac aaagacgcga
    21781 ttcaagcatt gctcttatta gcagaagtag cagcttacta taaaaaacaa ggcaaaacat
    21841 tgtatgacgg cttgcaaacg atttttgaaa catatggcta ctacgcagaa aaaacgattt
    21901 ctgtgacaat gagcggacaa gaaggctctg caaaaatctc tgctttgatg gaaaaattcc
    21961 gtgaagaagc accgacagaa tttgccggag caaaagtcat ccaaacagaa gacttcaaag
    22021 agttgacgag aacagaccgt gaaggaaacg tagaaaaact aacgacaccg ccatctgacg
    22081 tattgaagta tacgctggaa gaaaatggct ggatcgctat ccgtccttca ggaactgagc
    22141 cgaaaatcaa attttatatc ggtgtaaaag gaaactcttc agaagacgca gctgaaaaag
    22201 tagccgctta tgaagcgatt atcaaaaaaa tgacagaaga ataaaaaaac ttgagactga
    22261 tggaaaaagt ggtaaacttt agaagtcagt cttttttcaa gttggaggtg aatggattgc
    22321 cagacacgca aaaaacaaaa gtagagaaag tcagccaact attcaaaatg ttgagtgacc
    22381 ccactcgtct caaaattctg ctgtatctga aagacgggga acaaaatgtg acagcaatca
    22441 cccaagcagt agaaatggaa caatctgctg tatcgcatca actgcgttta ttaagagaaa
    22501 accatgtcgt caagtcccat cgtgagggaa aagcgatttt gtatagtctt gatgattctc
    22561 acgtactgga tattttgaac cagacgctca aacatgtgga acatcaataa cgataggtgc
    22621 aaatagagac aaactggact gtatcaacga atcttcttcg ctgacacagt ccagtttttt
    22681 taagaaaaag acaagaaaaa ctgttagtct gtttcttcgg tggtttcagt tccataataa
    22741 ctatgatctt ctaggtctaa agcattcaaa ataagtgaag ctgtttcttc gatagataaa
    22801 gaagcgacat tgattactac gcagcccaat ttttcataaa gatcattcgc aaaatcgagt
    22861 tcagctcgta ttttttcgat atcggaataa gcagtatcag gatttaatcc gtaagagcgc
    22921 atccgttctt tacggatatt gtttaaaata tctgggttat tcgtcagtcc cacgattttt
    22981 ttaggatcga tctcccaaag ctgtttggga atatgtgctt gtgggatcaa aggtagattg
    23041 gcaaccttta aatttttgtt agctaaaaac aagctcaaag gcgttttgga agttctggat
    23101 acgcctagca aaacgacatc tgcttctaga aacccacgag gatctttccc gtcatcgtat
    23161 tttacagcaa attccattgc tttgatccgt ttgaaatagt tctcattcaa taaatgaagg
    23221 gcgccaggtt gccgaatggg cttgatacca gttaagcgct cgatttctga tatcggcgga
    23281 gtcatcacat caaaactaaa taattgatgt tcttcacaaa aagaacgcgt catctcgatc
    23341 aattcttgat tgatcaatgt gtgtaggaca agggcttttt cttttagtgc atcttgcagt
    23401 gcctgcatca atgttttctc atctgtcaca aatgttctgc ggaataaatt gaactcaacg
    23461 gtaggatatt gggccatagt ggcttgcgct aattttgtcg cagtttctcc agcagagtca
    23521 gagatgacaa aaaaagtaat gatttctgta gaatcggaag acatggataa aacctcactt
    23581 gcattttttt tatgtttatt ataccatact ctagaatgtg tacttcgtaa tcattttgat
    23641 ttggaggaat aagtcatgaa aaaacagaca ttgattgaac aatcactaga aacattgaaa
    23701 aataacggtt ttaaatatac taaaaaaaga gaagcattgt taacttattt agtgaagcgc
    23761 aatcgctacg tatccgctaa agaagttttt gactatatga atcaggaatt caaaggagta
    23821 agttacgata cgatttatcg aaatctccat gattttgcag aaatcgattt actggaagaa
    23881 accgatttaa atggagagat gaagtttcgt tttcattgct gtcaagggga aatcggccat
    23941 caccatcact ttatctgtac ggtgtgcgga acaacgaaag aaatccatat gtgtccaatg
    24001 aattatttcg aagaacagct cggtggatgt acgatcgaag ggcatagatt cgagattttc
    24061 ggaagatgtg aaaattgtca ataaggattt aaataaaata acatttgata attttaaaaa
    24121 aaagcaagat ttccttgacg gtcaaaacag cattagatat aatatttaca ggacagtctt
    24181 gttttttgag atatatttca ataacaatta cggttacttt ttaagctcgg agggagggaa
    24241 ttaacatgtc aaaaacagtc gttcgcaaaa acgaatcact tgacgatgct cttcgtcgct
    24301 tcaaacgttc cgtgtcaaaa gcgggtactt tacaagaatc tcgtaaacgt gaattctacg
    24361 aaaaaccaag tgtaagacgc aagaaaaaat ctgaagcagc tagaaaacgc aaaaaattct
    24421 agttctgtga tgaattggag ttggtagtgt gtcactacta agtagattaa acgatgacat
    24481 gaaaacagcg atgaaagcga aagataagga atctttacaa gtcatccgaa tgatcaagtc
    24541 atctatacaa aacgagcaga ttaaagaagg tcacgacttg acagaagaag aagaattaac
    24601 ggtgttgtct cgcgaaatga aacagcgacg tgattctttg catgagtttg aagaagcagg
    24661 acgtgacgat ttagctgaaa aagtaaaaag tgaaattgtg atagtcgaaa aatacatgcc
    24721 ggaacaactt tccgacgaag aaattcgaca acttgttcaa gaagcaatca cacaaactgg
    24781 tgcttcatca atgaaagaat ttggcaaagt catgggtgcg atcatgccta aagtcaaagg
    24841 aaaagcagat ggaaaccaag taaatgccat tgtgaaagaa ctattacaat cttaatttta
    24901 tcatttaaag gctcttcgtc aatgagtgtt ggtgaagaaa ttc
//
LOCUS       DASNNU010000035        24697 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.035, whole genome shotgun sequence.
ACCESSION   DASNNU010000035 DASNNU010000000
VERSION     DASNNU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.035"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(233..1081)
                     /locus_tag="U8116_001351"
     CDS             complement(233..1081)
                     /locus_tag="U8116_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN8434356.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="U8116_001352"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="U8116_001352"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEN8434357.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="U8116_001353"
     CDS             complement(3582..3761)
                     /locus_tag="U8116_001353"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEN8434358.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="U8116_001354"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="U8116_001354"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN8434359.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /locus_tag="U8116_001355"
     CDS             5909..8188
                     /locus_tag="U8116_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN8434360.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="U8116_001356"
     CDS             complement(8281..8928)
                     /locus_tag="U8116_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434361.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="U8116_001357"
     CDS             complement(9107..9826)
                     /locus_tag="U8116_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN8434362.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="U8116_001358"
     CDS             complement(9957..11108)
                     /locus_tag="U8116_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEN8434363.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="U8116_001359"
     CDS             complement(11325..12065)
                     /locus_tag="U8116_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN8434364.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="U8116_001360"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="U8116_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEN8434365.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /gene="yqeK"
                     /locus_tag="U8116_001361"
     CDS             complement(12449..13042)
                     /gene="yqeK"
                     /locus_tag="U8116_001361"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEN8434366.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13676)
                     /locus_tag="U8116_001362"
     CDS             complement(13026..13676)
                     /locus_tag="U8116_001362"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEN8434367.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="U8116_001363"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="U8116_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEN8434368.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="U8116_001364"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="U8116_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEN8434369.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="U8116_001365"
     CDS             complement(15119..15646)
                     /locus_tag="U8116_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEN8434370.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="U8116_001366"
     CDS             complement(15826..16611)
                     /locus_tag="U8116_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEN8434371.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /locus_tag="U8116_001367"
     CDS             complement(16604..17473)
                     /locus_tag="U8116_001367"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEN8434372.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="U8116_001368"
     CDS             complement(17474..18853)
                     /locus_tag="U8116_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEN8434373.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="U8116_001369"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="U8116_001369"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN8434374.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="U8116_001370"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="U8116_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEN8434375.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="U8116_001371"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="U8116_001371"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEN8434376.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="U8116_001372"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="U8116_001372"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEN8434377.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="U8116_001373"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="U8116_001373"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN8434378.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="U8116_001374"
     CDS             complement(22811..23035)
                     /locus_tag="U8116_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN8434379.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="U8116_001375"
     CDS             complement(23088..24050)
                     /locus_tag="U8116_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEN8434380.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="U8116_001376"
     CDS             complement(24060..24500)
                     /locus_tag="U8116_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN8434381.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6786 a         5472 c         4278 g         8161 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattt
//
LOCUS       DASNNU010000036        24295 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.036, whole genome shotgun sequence.
ACCESSION   DASNNU010000036 DASNNU010000000
VERSION     DASNNU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.036"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(55..789)
                     /locus_tag="U8116_001377"
     CDS             complement(55..789)
                     /locus_tag="U8116_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN8434382.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="U8116_001378"
     CDS             1116..1526
                     /locus_tag="U8116_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEN8434383.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="U8116_001379"
     CDS             1519..2220
                     /locus_tag="U8116_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEN8434384.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="U8116_001380"
     CDS             2220..3806
                     /locus_tag="U8116_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8434385.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="U8116_001381"
     CDS             3825..4046
                     /locus_tag="U8116_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEN8434386.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="U8116_001382"
     CDS             4043..4804
                     /locus_tag="U8116_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEN8434387.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="U8116_001383"
     CDS             5184..5693
                     /locus_tag="U8116_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN8434388.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="U8116_001384"
     CDS             complement(5768..6307)
                     /locus_tag="U8116_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEN8434389.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="U8116_001385"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="U8116_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEN8434390.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="U8116_001386"
     CDS             7320..8171
                     /locus_tag="U8116_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEN8434391.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="U8116_001387"
     CDS             8199..8834
                     /locus_tag="U8116_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEN8434392.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="U8116_001388"
     CDS             8854..9627
                     /locus_tag="U8116_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN8434393.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="U8116_001389"
     CDS             10035..10832
                     /locus_tag="U8116_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEN8434394.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="U8116_001390"
     CDS             10810..11223
                     /locus_tag="U8116_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN8434395.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="U8116_001391"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="U8116_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN8434396.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="U8116_001392"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="U8116_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEN8434397.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="U8116_001393"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="U8116_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEN8434398.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="U8116_001394"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="U8116_001394"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEN8434399.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="U8116_001395"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="U8116_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEN8434400.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="U8116_001396"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="U8116_001396"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEN8434401.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="U8116_001397"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="U8116_001397"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEN8434402.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="U8116_001398"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="U8116_001398"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEN8434403.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="U8116_001399"
     CDS             21907..22485
                     /locus_tag="U8116_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN8434404.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="U8116_001400"
     CDS             22537..23019
                     /locus_tag="U8116_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEN8434405.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="U8116_001401"
     CDS             23136..23510
                     /locus_tag="U8116_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434406.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23885..>24295)
                     /locus_tag="U8116_001402"
     CDS             complement(23885..>24295)
                     /locus_tag="U8116_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN8434407.1"
                     /translation="IAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNY
                     PKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLER
                     FLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT         7912 a         4357 c         5203 g         6823 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgggagcgt caataatttt gtgtaaataa attgtcctcc tgcaaaataa
    23881 ttagttactc agtaaacatt gaaactaatg tatcggttac ctgttgaaaa cctttatggc
    23941 ttctgtttag aaatttttga ttgtatgtat caaaaatgct gactagaaag cgttctagtg
    24001 attcttcatt ttgaaactgc tcttttctac ggctgtatct tttaatttgc ttattgaaag
    24061 actcgattag attggttgag taaatggttc tacgaatgct aggtggaaaa tcataaaaag
    24121 ttaataagtc ttggttttct atgagtgact gcgtcacttt aggatagttt ttcttccatt
    24181 tctcaatcat gccggataag aaggtattcg cttcttcttt tgagttagct tgataaacag
    24241 ccttaaagtc atcacagatt tcttttcggt ctttgacacg tactttatga gcgat
//
LOCUS       DASNNU010000037        23874 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.037, whole genome shotgun sequence.
ACCESSION   DASNNU010000037 DASNNU010000000
VERSION     DASNNU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.037"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="U8116_001403"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="U8116_001403"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEN8434408.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="U8116_001404"
     CDS             1013..2260
                     /locus_tag="U8116_001404"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEN8434409.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="U8116_001405"
     CDS             2336..2680
                     /locus_tag="U8116_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434410.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="U8116_001406"
     CDS             complement(2797..3123)
                     /locus_tag="U8116_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434411.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3738..4940)
                     /locus_tag="U8116_001407"
     CDS             complement(3738..4940)
                     /locus_tag="U8116_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN8434412.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            5057..5572
                     /locus_tag="U8116_001408"
     CDS             5057..5572
                     /locus_tag="U8116_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN8434413.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5771..6217)
                     /locus_tag="U8116_001409"
     CDS             complement(5771..6217)
                     /locus_tag="U8116_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN8434414.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6264..7742)
                     /gene="msr(C)"
                     /locus_tag="U8116_001410"
     CDS             complement(6264..7742)
                     /gene="msr(C)"
                     /locus_tag="U8116_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN8434415.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(8096..8725)
                     /locus_tag="U8116_001411"
     CDS             complement(8096..8725)
                     /locus_tag="U8116_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN8434416.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(8709..9173)
                     /locus_tag="U8116_001412"
     CDS             complement(8709..9173)
                     /locus_tag="U8116_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN8434417.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9660..10928)
                     /locus_tag="U8116_001413"
     CDS             complement(9660..10928)
                     /locus_tag="U8116_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN8434418.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(11159..11896)
                     /locus_tag="U8116_001414"
     CDS             complement(11159..11896)
                     /locus_tag="U8116_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN8434419.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11963..12532)
                     /locus_tag="U8116_001415"
     CDS             complement(11963..12532)
                     /locus_tag="U8116_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEN8434420.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12660..13532
                     /locus_tag="U8116_001416"
     CDS             12660..13532
                     /locus_tag="U8116_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN8434421.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13874..14746
                     /locus_tag="U8116_001417"
     CDS             13874..14746
                     /locus_tag="U8116_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN8434422.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14743..16701
                     /locus_tag="U8116_001418"
     CDS             14743..16701
                     /locus_tag="U8116_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN8434423.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(17033..17884)
                     /locus_tag="U8116_001419"
     CDS             complement(17033..17884)
                     /locus_tag="U8116_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN8434424.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            18007..18474
                     /locus_tag="U8116_001420"
     CDS             18007..18474
                     /locus_tag="U8116_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEN8434425.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18737..19219)
                     /locus_tag="U8116_001421"
     CDS             complement(18737..19219)
                     /locus_tag="U8116_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEN8434426.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(19319..20182)
                     /locus_tag="U8116_001422"
     CDS             complement(19319..20182)
                     /locus_tag="U8116_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN8434427.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20780..21655
                     /locus_tag="U8116_001423"
     CDS             20780..21655
                     /locus_tag="U8116_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434428.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(21800..21952)
                     /locus_tag="U8116_001424"
                     /pseudo
     CDS             complement(21800..21952)
                     /locus_tag="U8116_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(22220..23551)
                     /locus_tag="U8116_001425"
     CDS             complement(22220..23551)
                     /locus_tag="U8116_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN8434429.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(23700..>23874)
                     /locus_tag="U8116_001426"
     CDS             complement(23700..>23874)
                     /locus_tag="U8116_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HEN8434430.1"
                     /translation="KGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEYSVSRHL
                     YQDFVFEAVFVEK"
BASE COUNT         7360 a         4824 c         4216 g         7474 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tgcaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg atcggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caatcagctg accctcggta ttagctcaaa aaacggtaaa
     3421 atatgagaag ctatttttcc gttttgtttc tctaatactt aggataaaac gctgatttta
     3481 caacctctgt taaacggtgt tcaatcagct gtccttcggt attagctcaa aaaacggcaa
     3541 aatatgagga actatttttc cgtttgtttc tctaatactc aggacaaaac gctgatttta
     3601 caacctcctg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg
     3661 aaaaatatga gaagctattt ttgtttggct gttcttatga cttgatgcag gacagctttt
     3721 tcacaacctc tgatttctta ttcttcttca tttgtagcaa aataattacg gtgataaatg
     3781 atacaacata cactgataag aacaatggca cctgcaaaat aaaacgcata gctttcacca
     3841 atatgttcac caatcacacc tgcaataaat ggtgctaaag ctccaccgaa aaagcggaca
     3901 aaactatagc tggacgaagc aacgtttcgt tgcagacctg gtatttccat agcaatcgtc
     3961 gtcaacagtg tattgataat tccttggaaa aatcctgcta acacaatgct gatcggtaca
     4021 aggactggca tagaagttcc agctcctaat aacagcaaac aaataatgaa taagacaaat
     4081 gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc aacgaaaatc
     4141 gaagcaatag ccagaagcac accccagcca aaaaatacaa aaccgacttc taattcagaa
     4201 tagccaatca ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa attatataac
     4261 aatgcgatta gtccaactga acgaagttta tgattgctta acgctttgac accttcccaa
     4321 aggcctactt ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag ggctataaaa
     4381 ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac gccatgaacc actgcccaag
     4441 accccaccga ctagagggcc aacagccatt ccgcagccaa gtgctgcttc atacatcatg
     4501 attgcttttt ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc agtagagaca
     4561 aataaagcat tcccgacacc ccagccggca cgtaacccaa tcagcattcc gatattccca
     4621 gataatccgc caagaccagc gaatacaacg atgataaaca atcctgtcat caatgttttt
     4681 ttcaaaccga tccgactaga taagaaacct gtaaatagca tcaccacacc agtgactaac
     4741 atgtaactag taaataacag ggtcgtttca gctggcgtag cgtttaactg tgcagcaatc
     4801 gtttttaaga ttgggtctac tagacccact cccataaaag cgatcataca ggtagctgct
     4861 acaactagcg ctgaactttt cccctttgtt tcttcatttg tttgttcttg tatatctatt
     4921 tgattctcca atatttccat ccctttctct ctattagcat catacatgat ttattttaca
     4981 tgtcaatatg cacatgtaaa acgtggaaac tttactgtga tataataaag aaaataagac
     5041 gagaggtagg atttttatga atactttagc ctatatatta ttatgcgtgc ttttatccaa
     5101 accacgttct gggtatgaat tgaagcagct gatcacaatt ttttgggaag cccatcacag
     5161 tcagatctat acaacgcttg ccaagttaga ccagcagggt tatgtgaccg ttttggacaa
     5221 agacgagcac tctcaaaaaa agatctacca cttgactgat gaaggacgtg ctcttgtaga
     5281 ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa gatgaatttc tagcaaaaat
     5341 ctatgctttt tcgacgctcg ataaaacaac agctactgga ctgctattca ctagagaaca
     5401 acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg aatgacctct cgctagacaa
     5461 gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga attatgcttt gtcagcagga
     5521 actccaatgg tgtcagcaag taagagaaca aatgcagcag ttttttgagt aagggataaa
     5581 cagaaccttt ctagaaatat ctattgaaga gcaaattatc ttcaataaaa gagcaataca
     5641 gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc tatcgttcgg atcactattt
     5701 ttctagaact gcattcaaga gactatgaca aaaagcatgc caaggccttt tttcaaataa
     5761 cagtgttcca tcattcatta ctttgaattg cttttagcac acttcttgaa agcgggccaa
     5821 cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat tttccaagca ttcatataag
     5881 cagagaataa ttgatcaggt attccttgtt ccatgatcac accgaataaa gacatacatg
     5941 tcaccattcc taaaaccatc aagcatgaaa tagttaagat catttggacc ttgctttctg
     6001 gatttactgg tagtttaaag gcaatttttt tcgccagcac accgatgatg aagacatcca
     6061 aaatgaaagc caccacgaat ccaggcacta gaccaatcaa caaacttgat aaatgaagat
     6121 cttgatgcag ccataaatta tacgcgctca tccccaagac catcaaaaaa cacatcattg
     6181 ttgtaaaaat tattccttct tttttatttg taggcaatct gtaccactcc tttaaatttg
     6241 tcacgaaaat catcgtagca catttaaaaa tttctcgtaa gtactttttt tattttctgc
     6301 ccttttattt cataaacttc atcagccgta cgctcgacaa agtaagaatc atgagaagta
     6361 aacaaaatcg ttcccggata tatttgcatt agcttctcta aagcttcgat cgttgccata
     6421 tcaataaaat tagtcggttc atctaacagc aacacattac ttggcttagt aaaaagcaaa
     6481 gcaatcgtca gacgggtcgc ttctccccca cttaatgtac ataacggttt cgtcaaagtt
     6541 tcctgagcaa accctaagtg attcagcaaa ctacggacaa gcgattctga aaactctgtt
     6601 tgtctcatga caaaacgaat gatggattct tcagatgtca tttgataacc cttttgctga
     6661 tatacttgaa agcttacttt cggagagaga aggattcctt ctccttgttt gcggatatgt
     6721 tctaataagg ttgtcttacc tgaaccattt tcaccgacaa tcgcgatttt tttacctaaa
     6781 gaaaactgaa aatcaccatt taccagtaat gttctcccac ttctttccaa ttggacattt
     6841 tgtcccatga tgggatagcg gctgtggagt tcaagagatt ttggtactgg aaaacggatt
     6901 tgtttcatct gctgcggctt ggttgtttct cctatttttt ggagtcttct ctctaatgtt
     6961 ttcgcattct tttggatggc tttttgtaca ctgtcttttt gtttagagga agacaaccga
     7021 tccagtctga tttgttgctt tttcttttta ctggacactt tttccaactt ttccgcttgt
     7081 tttttctttt cttggatcga tttttccaat cgttttttct ctttcatgat cgactctgct
     7141 tctcgtagct gagtcttttt ctccaattcc ttttgagtga gataggcact ataattcccc
     7201 gaaaactctc ggatttctcc gtcttttact tcccagattt tcgatgctag ctcatctaga
     7261 aaatagcgat catgactaac aaaaaccagc gtaccataat aataacgaag ctcttcaatc
     7321 agtcgatcaa tactttttct atctagatgg gttgttggct cgtctagaag catcccttgt
     7381 tcataagaag aaagaagacg tatcaactcg atctttcgtt cttcccctcc gctccatttc
     7441 cgctctgaag tttcttccag tcttaactgg aaatgactgg ctaattccaa atttaggtcc
     7501 tttgcttcag cgagataggt taattgaggc aggtagttga aggtgatctc tgtttggatt
     7561 ttcccatgat cgggaaaaag ttcaccggct attagtttga gcaaagtcga tttaccgaca
     7621 ccattttctc ctatgatccc tatacgatct tgctgataga cacgaagaga atcaatagat
     7681 agttctagct ggttgccaaa gctgacttgt agatttgtta tatttactgc taaattttcc
     7741 atatgtttta cactcctatt tcatgtaaaa caacaattat cgatactaaa aaagagcaga
     7801 aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta ctctgaacga aataactatg
     7861 actaaatcac caaatctgtc atagctttca cctattctac tgtcgttcga aaaaatggac
     7921 atactccatc catgaaattg ttgtttatac atgtcttttg aatttattta gttgttattc
     7981 aaaagttcga aacgtaattt catcgatgca gtcatttttc cgacctcctt ttttattagg
     8041 ttaccacaca cgattcaaaa aaagcaatag ctactgaata gtaactgaaa accttttatt
     8101 cgaacatagt gatcttttct ggtgctaaac gaacagactc gtaaccttct tctaccgctt
     8161 ttcctattga taaaataata actggaacat aacggtcttt atccaaacca aatgcctctg
     8221 ccaactgatc tgcttcgaat ccaccaattg gatttgtttc atagccatga tcacgcgcaa
     8281 ctaacatgaa ttgcatagcc gctaagctag aatcgacttt gactacatcg ttcatttctt
     8341 ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa ttgttgatct ctgacttctt
     8401 ttggcatctt ccctgattcg taagcttggt tgtagatttc ttcgccatat tcgtaacatt
     8461 gcatatcgcc aaaaatcatg accattgcag aagaagtatc attttgtctc gtattgaaac
     8521 ggatcaaagg acgcaagatt tcttttgctt cctcgctttc agcgaccaca aagcgccatg
     8581 gtttcatatt gacagaagaa ggtgcagtcg tcgcttcttt gatcatttcc agcatttctt
     8641 cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga tcttctacca gtcataattg
     8701 ttgaaaaatc atttttttgt tccattatta ttaacctctt tcgtttctgt tgttatattg
     8761 caaagttggg tattcaattt atctaaaagt gttagaaatt gtttcatttc tacatcgctg
     8821 aaagccgggg atagcacgga acctgtatct ttatggtcca tttcacaatg cttgattttc
     8881 ttctgggctt tttctgttag cgctacatac acttcccggt tgttctgagg atttctctga
     8941 cgcgaaacat agccatttgc ttccaatacc ttcagatgac gagtaactgc tccttgatca
     9001 atctgcaact tttcttgcaa actgctttgt agcaatggat tttcttcttt cagtctcatc
     9061 atgatctcgt atctagtaag acttataccg atattttctt caaagatctg gacgattttt
     9121 tgatcggcta atttgatttg atataaaagg tgactaatct cttctaaaga cataatttcc
     9181 tcctttctaa taattgactc gtcaacgatt gacttgtcaa taatagaata aaaaaagaaa
     9241 aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc aatagaaaag tatagagaaa
     9301 agaaattttt tgtagaatga acggaaaaaa tgataaagaa agcacaacgc taaaaccaag
     9361 attattgatt cttatcaaac tatagaagat agctcttctg cagtagataa ttttacaccg
     9421 ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg aatattagaa attggatcag
     9481 tatgcttaaa cagtaataaa aaagttatga caagatgttg tcacaacctt ttttgagtaa
     9541 acggtgttca aaaacttgca tctgcggtaa taagttcaac caaacaaaaa acatgaaaat
     9601 ctgtttttgt ttggctgttc ttactacttg atgcagggca gctttttcac tacctctttt
     9661 taataaccat aaaatcctat tccttcatat ctccagccca tcggtgttag ccaatctctt
     9721 tccttagcac tcaaggtata gtgatgggta ccagtttttt catttggatt aaatagtcga
     9781 taaattggct tggaaccacc agaataaaaa cttagacctt cataacgcca accgattttt
     9841 gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat ggtctccact gtaaggattg
     9901 tataaacggt agacaggttg tccgctgttt ggtgcttgcc aagcaatccc ttcatattcc
     9961 cagcctacag ttggcagata atttttttct ccggcattca atgtatataa atgttctcct
    10021 gtatgacggt tatacagacg atacatcgga gaagttgaag ttggtgctgt atcatactgt
    10081 gtcagattat aggtttcgat tacattgttc aatgtagaac cataattagg cgcagtggca
    10141 taacgacctg ttagccaagc cgtagcatct ttgtaagagt tcgtattgct tttccatgca
    10201 ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct tcaataccgt tacgttatct
    10261 tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg cattcaccgt aatccattgc
    10321 ccattcacat attccgaagt aggcatcgta acagattggc cattatagct tcctttaatc
    10381 ccaaatagat tgtaattagg ggcagaagcc aaagtactgt tcccccagcc actttcaacg
    10441 atggcttgag caatcatgac tgatgcatat aagtcattgg cacttgccaa actttgtgca
    10501 gttgaggcaa tactatcaat gaacgcttgt tgagaattag cagatgagcg agcataaaaa
    10561 gcatagccag tatagttttc gaattcagac aaatctgctt gatgggtatg tgtcaatgtt
    10621 tgcccatctg tcaattggtt cgtgatttct tctgcattct ctggattgtt ctgagcttct
    10681 gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt cagttgttgg ttcttcggtt
    10741 gaaattgtat tttctgagac agtagctgac tgcaaagtac tttctgtatc gttttcaatt
    10801 cctgtttctg aagaactcga tgtctgagtt gcgacagttt ctgtcgattc ttcagctagc
    10861 actgttgtgt tggcaactgc tagatttcct aaaagaataa cgctaaataa taaattcttt
    10921 gctttcatct tttttctcct ttgaattatg aaacttttcc taaataacag tgtaactaaa
    10981 aaagtaaaag caagtcaatg tccatattta aataacattt gtttcttttt aataaatttg
    11041 ctttttctgc ataaaaagac gtaaatgaag aatattcttc tccattcacg ccttaaattg
    11101 atggtcgttt gaataactat ttaacaagta attctctctg gatattatcc gttatggatt
    11161 atacttcctg tgtcgtcgga cggataatca tgttgctgac agacatccgg tctggcgttt
    11221 ctatcgcaaa tgctacagca gaagctatat cttcagatgt taatccccat tctttttgcg
    11281 cttcatgaag ttcaagagca gcctttttgt cgctgatggt ttgatataat tcagtttgaa
    11341 cagctcctgg agagatgatc gtagatttga tgttattttg ccgttgctct tgacgtaatc
    11401 cttccatgat tgcacggaca gcaaattttg ttccgcaata aacggcggaa tcaggataaa
    11461 caacatgtcc cgccacagaa tcagttgaaa tgatatgccc agatttttgt ttttccatga
    11521 ttggaagtac ggcggaaatt ccatttaaca cacccatgat gttgatatca agcatatttt
    11581 gccattcatc acggtgacct tcaactaatg gagcggttgg catgattcct gcattattgt
    11641 acaatacatc gattcgaccg aacttttcca tcgctaaatc gataactgct tggacttgtg
    11701 caaaatctct tacatctgct gtttgaatat atagctcagc ttctggtaaa gattctttta
    11761 ttgcttttag gcgatcttct cggcgtgcag ctataactag tttagcccct ttttctgcta
    11821 atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc catgatgaca atcacttttt
    11881 ctgataaatc attcattcta ttctcctcct gttttgaaaa atctcttgct tatactcact
    11941 atctcttatt tcatcttact gttcaaataa tccgttcgac aggtttgacg attttggcag
    12001 ggattcctgc aacgatggta ttgtctggga catctttcgt aacagttgca ttcgctgcaa
    12061 caatggcatt ctcgccaatc gttacgcctg gcagaatagt tgctcctgct ccgatccaag
    12121 catttttctt gatgtggatt ggctttactg acaagcctct tcgttctttt ggatcggtta
    12181 gatggttcac cgtgatgagg ttgacacgag gaccaatcaa tactgaatct tctaaacaga
    12241 tgccacctaa gtcaacgaac atcacttgac gattgataaa gacatttttc cctatactaa
    12301 tatgtttgcc gaaatctgta taaaaaggca gcagaacagt caccgtatca tctactttgt
    12361 cactaatgat attttctagc cgtttgcgga tatctgctgg ttcatggtac ccagtattca
    12421 gcgctgctaa aggctcttcg ttttccgctt ggatctgatg gatcacttca aagagcggat
    12481 ccgttactcg aatctcttta ttgacgattt gtttttcgat gaggcttgtc attttgctcc
    12541 ctccttttat ccttacttta taacggacgg cttatgatgt ctaatactta taaaacatac
    12601 ttaactattc cttttaacta tacttagtgg tatgcttttt taaaaacagg agggaaaaaa
    12661 tggatattcg tatactgacg tattttttag cagtagctag agaaaaaaat atcagccgag
    12721 cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa acaactgaaa gatttagaag
    12781 aagaacttgg tgtgacactt ttcactcgag gctcccgaga gatacagttg acggattctg
    12841 gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa acggcgaaca
    12901 atttaacaaa tgacgcaatc atctctggag aaatctacat tggcggaggc gaaactaaag
    12961 caatctcttt catttctgat agccttcatt cgttgatcaa ggcgcatcct gatatccagt
    13021 ttcatttata tagcgggaat gcagatgata tcatagacaa actggatagc ggattacttg
    13081 attttggact cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt cgactgccag
    13141 cccaagatcg ttggggactt ttgatacatc ggaatcatcc tttagcaaag caaacagtca
    13201 tccatccgca ggaactgaaa actgttcctt tgatcatttc ccggcaaacg tcagtggaca
    13261 gtcaactagc tgaatggcta ggtttcagca tagatcagtt atcgatcgtc ggaacatata
    13321 acttattgta caatgcttct ttgatggtcg ataacgggac ttttggtgca ttagctctag
    13381 atgggatcat caatacttat gggacggaaa aagtattcat cccactcgat ccgccattac
    13441 aagcttcact gaatctcatt tggaaaaaag accagcctct ttctcctgct gctcgtaaat
    13501 ttttagatgt cctaacggag aaatacagtt aagtagagtt tttttccttc gctttcagct
    13561 ttttaacaac gaataagtta agcaaaagat ccaatgatta aataacataa gtttcttaat
    13621 ttgttccttc cttttatttc tgataattcc agtaaattac tataattata aaaaaagaat
    13681 tgacaaaaca taactgtcta tataaaattg attacgttaa caataattcg tttcatgtga
    13741 ctagatcata ctaggcatga aaaatctctg aggaacatgg tttccctagg aggtttttcg
    13801 tgtctatttt tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc aaatcttcaa
    13861 aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat tggtagatga
    13921 cggtggacag gaaaaagtag ctattggaaa agggattgga tttgacagaa aaagaaatga
    13981 tttgattttt gatcgtgatg tcgaacggat atttgtcatg gaaccagaag gtcaaatcaa
    14041 gttgcaaaat ttacttaatc agattgatga acgcttttta tttgcagcag aaacgatcat
    14101 tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa catgtattga tcgctttgac
    14161 cgaccacatt gctttttctg cggagaacat taataacggt atcgtcatcc gcaacaaact
    14221 tttaagagaa atcgaagtgc tttacagtga tgaattcagt attgctcaat gggcagtaga
    14281 gtatctgaca aaagagcttg ggattccata tacttatgac gaagcaggat atatcgcaat
    14341 ccatattcac agcggacgaa gcggtcggaa caataaccat cgcagtatcc gtgaagtgac
    14401 gattgtatct gacatcatcc atttagtgga atcagaattg gaaacagata tccattcaga
    14461 accattttca ctgaattatt caagactggt gaatcattta cgactgcttc tacaacgaac
    14521 ccatgcgcaa caatatgctg ttttagacac ggaaatcgta gaaatggtca aacgaaaata
    14581 cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag aataccagct
    14641 cgcgatcacg aaagaagaac tgggttactt ggccatccat atcgagcgtt taagaagcgc
    14701 aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa aattgaaagc atatctacaa
    14761 cgaatgggac gttcccttca acttccagtt gcggtcttac ctgctgctgc tttactagta
    14821 gggatcggaa actggtgggc atcttatagt aacgacattg tcgctcattt tttacaagct
    14881 ggcggtaacg cagttttagg tcaattacct ttgctgtttg ccgttgggtt agcactaggg
    14941 atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt tgaagttcca
    15001 gtaaatgttt taaaaactga ttcagtagct actttattga atatcaaacc agaatcagtt
    15061 gatccatctt ttgctcagat cagtaacgta tttattggta ttattgccgg attgattgcg
    15121 gcagctcttt ataatcgttt ccacgaagca aaattaccga tggctttatc tttctttagc
    15181 ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt tgctgatcag tgcagtgcta
    15241 ctgctcgtat ggcctccggt ttactctgct ttagtttctt ttgctaaatt cattttgagt
    15301 ttaggctggg gtggtgcagg tctttatggc ttcttcaacc gtttactgat ccctacagga
    15361 ttgcatcaag caatgaactc cgtgttctgg tttgacgtag caggaatcaa tgatattggt
    15421 aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc tggatttttc
    15481 ccaatcatga tgttcgggct tccagcagga gcatatgcta tttacagaaa tgcacgtcca
    15541 gaaaacaaag caaaaacagc ttccttgatg ttagcagcag cttttgcttc attctttact
    15601 ggtgtaacag aaccacttga attctcattt atgttcgttg cttggccatt atatgtcttg
    15661 catgcaattt tcactgggat ttctttagca gtgagtgcat tcttccattg gacagcagga
    15721 tttgccttta gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc gattgcgaat
    15781 cagccattga tgttgatcgt ccaaggatta gtaacagcag tgatttacta ctttggcttt
    15841 aactttgcta tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc agatctttca
    15901 gatgacgata ctgcaacgac taattcagac aataaatatg cggcacaagc aagcaaaatc
    15961 tacgctgcat taggcggagc cgacaatgta acttcgatcg ataactgtac tacacgcctt
    16021 cgtttgcaag tcaaagatac aggcctgatc gatcaaaata aaatcaaagc cactggcgta
    16081 cctggtatga agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac agaagtacaa
    16141 ttcgttgcag atgagatggc aaaactacat ggtggtgctg caacacgacc tgcacaaaca
    16201 aacaccgtgg ttaaaacaga aacatttgcc ggtggaacga tcgaacaaga tatttacgct
    16261 atcgcaaatg ggaaactgat tccaatcact gaagtttcag atgatgtatt cgcggaaaaa
    16321 atgatgggtg acggctatgc tgtattacca gaaaatggag aaatcttctc tccaatcgct
    16381 ggtacgatca caaacatttt cccaacgaaa catgctgtcg gaatccaaac agatgctggt
    16441 atcgaagtct tgcttcatat gggcatcaac actgttgact taaaaggcga accattcact
    16501 ttatatgtaa aagaaggtca aaaagtcgca agaggtcaat tgattgcttt agttgatctt
    16561 gccgctattc aatctgcagg caaaaacacg gatatggtcg ttgtattcac aaatggcgac
    16621 aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga taaaatcggc
    16681 tctgtttcca acaaagcctg agccatcgtc ttccccagac ttttttatcg ataaacaaac
    16741 aggagctgag acaaaaatct tgtctcagct cctttttccg aataaaaacg gcgttctcct
    16801 gccaactccg cggtaataag tccagccaaa cgtaacatat gaagagctat tttttatttg
    16861 actcttctta ttacttgatg cagaacagct tttttacaac ctctgataaa cggtgttcaa
    16921 aaaccagttt atcggtatga agtcaaattt atgcaaaaat gtggaaagcc attttcacaa
    16981 aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata cattattcct
    17041 gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc aatacttttt
    17101 ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg atcgctaaaa
    17161 acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca aacgctgcat
    17221 cacttgcttc aataatttct aactttttac ctgtcgcacg ctctaatgcc ttggctaatt
    17281 cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca ctttctacta
    17341 gtacttttgc tgcagcttct gcatattcac gtttcaatac ccaacctgtt tttccatttc
    17401 ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt aattcattct
    17461 ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta atcatttttt
    17521 cagtgaattg gtgatctggc gctaatacac tgatcgagtg atcggcatct gcaaaacttg
    17581 tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc ttatgttcgg
    17641 cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct ttcaacgctt
    17701 ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca gcttctttca
    17761 gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt ggaaccatct
    17821 tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt actaaataat
    17881 gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta catttataaa
    17941 ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat gaaaggatga
    18001 caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc ctatattgaa
    18061 atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat cgaaacaaat
    18121 gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat ctttacaaaa
    18181 accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt actagatgtc
    18241 tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac aaatccaaat
    18301 tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg gctgcagaaa
    18361 aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa aatagccgtt
    18421 ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt ataattctct
    18481 ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta ttctccgggg
    18541 gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag cgctgaagtc
    18601 atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc tgaaaaatgc
    18661 aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa gcgctacttt
    18721 cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct cccatatatt
    18781 tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag gcgtaatctt
    18841 ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt aattcttcag
    18901 gcgattctgc taaagcgatt acaacaagat acgttccttt tgggaatggg atgattcggg
    18961 tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt ttcccgttat
    19021 agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat gcatctattg
    19081 ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa ccgacaaaat
    19141 ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct ggtttttctt
    19201 cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca tcttacataa
    19261 aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta ttttttaact
    19321 atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa tcctctcccc
    19381 attttgtaaa agagatctcg ggagcttcga ctaactcata atctgaagaa ggaagccatt
    19441 ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct ttattaggaa
    19501 aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac ggattttctt
    19561 ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag cgggctccat
    19621 caaatccata agaaacgttg attacttgat ttggatataa atctcctaac ttatgcatct
    19681 cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga ttgactcctt
    19741 caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct ttcttttcga
    19801 tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag cgtggaaaag
    19861 atttttgtat gccgtatctg ctgatttctg aaggcagata tcctgaccaa tcacgaaaag
    19921 ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct gtcactcttg
    19981 atccatctag gagatcgcga ttagccaaag ccaatctacg gtttttgata tagtccatta
    20041 gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag attcctgtat
    20101 tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg taatcgatca
    20161 attggttcag atctttaagc atatttatct tcctccttgc ttgccttttt atcatattat
    20221 caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta gaaaatgttg
    20281 tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt attatctgcc
    20341 tatctatgtt ttacctttag aaaaataaat tttggggaag acacattcta agtaaacaaa
    20401 gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg tacaaagcaa
    20461 attgttgttc aaactgctga tcagaacgct gcttggattt ttctttgcga tacatcatac
    20521 ctcttttatc aattggaaca attatttatt tctaacaaaa tcacttcatt tttgtcagat
    20581 aaataaaaaa caaaaaaagt tccatcttta aaacacgcta ttcttttact ttttctcaca
    20641 ttctctaaga aaagaaaagg agaatgaatt tttgacaatt cgagcaaaag tgattatcaa
    20701 atatgtaaaa tcagctactt ttttcttaaa cgtttcttta tactaaatat aaataagtgt
    20761 ttcatgaaag gagaactaga tgaataattg gcttgagtat tttcctgaaa acgttttgga
    20821 acgcggttac agctatcatc tgcacggttt tgtccgtcat ttgaactata caagcaagta
    20881 cttgtccgca acagtttctg gcacggaaga ttataaggta gtgatcactt gggatgaaaa
    20941 gaccaacatg acttgtgatt gtctatatgc catagaagga aaaaaatgca aacacatggc
    21001 tgctgtctta ttcgcttacg aagaacgtcc aataaaaaaa tccaactact cactttccga
    21061 actctcgtcc cttgtttcct ccgctagttc ctctcttgta cgggagcttt taacagagat
    21121 tcttatagaa catccgcaat ttatcgaacg cttcaaagtt aagatgccat ttcatgcaat
    21181 aaattattca gataaattaa caactatcat ccataaatac gatcatatta tcaagaaaaa
    21241 caaaaacagg aaaacagcga agttcatcat ggagatgcga aagttcatcc aagaagcagt
    21301 tgaatcgctc attcagcaaa atgcctatct tcccgcattc gaattgatca atgaagtgat
    21361 tgctactctc gaaacattct attgggagcc tgaggatgaa cgaacccttc ttctgataga
    21421 ggactgttat tatttatgga aagaattatt agcagaagca cctcatgcag aaaaaaggca
    21481 aatgttctct tggttcgtct gccaagtcga tcatactgac gcctcttatt caaaacgcta
    21541 ctccataaaa atcctgaaag aagactttcg ggaaaaagaa ttttctaacc aaaagaaaaa
    21601 aatagataaa aaaactaaag aaacggtaaa gaaagacgat ttcaatgaaa aatagcaagt
    21661 aaacgataac aaccatccta aatccacaag aaatacagca gaccttgctt taaaaacacg
    21721 aatcatgcaa aggatgaagt aaagaagact taacgcgtac ccaatattag tcatttgact
    21781 attaggtacg cgttttgttt tataagtcat gacaaatttc cttcgttgct gggtagattt
    21841 tccggcattg ttgatagatt ttatgatact tttctacatt ttgtaagaga ggttccactg
    21901 catctgtata ttggataaaa ttctgacaac aactttcagg tgtgtcgaac cactctaatc
    21961 tttaaagata aatattaaaa taacgattaa taatattaac agtctttatt tttcaccacc
    22021 aaaaacctat ctttgcatat actaaataga cgtatataca cagataggtt tgtcttgtga
    22081 aaaataatga ttatccttac caaaaaatgt ttgtttttct acatgttcgt acttatcaaa
    22141 aaatatgatc cttattgaaa ttactggcta tttatattcc aacgacttct tgtgttaatt
    22201 tttgttatct catagtaaat tatgtttcat tttcacttaa tttagttgtc acgtgttcgt
    22261 caattcgtaa aaacggtaaa tatataaaca cacttgcggc tataattaat atttgtaaaa
    22321 cagctgctcg ccagtctccg gctgttgcta aaaatcccga tataactggt ggcgtagtcc
    22381 aaggaatttg cactactaca tgattaatta atcccacagc agttgcaaaa tatgctactg
    22441 tcaaagaaaa aatcggtgca agcacaaagg gaataatcag actgatatta aatacaatag
    22501 gcaatccaaa aataataggt tcgcttatat tgaatatagc gggagttacc gataatttag
    22561 caaagtcctt ataatctttt ctacgactga agataaatat tgcaatcaat aaagcaatcg
    22621 tatttcccga tccacccatt accgcaaagg tatctttaaa ggccatattg ataatatgtg
    22681 gaatttgctc tttgtttgca taagctgcca tattttcttg catattctga atcaaaaacg
    22741 gatctaacaa tgaacctgat attactgctt gatggatacc aaatccaaaa aatagattag
    22801 caatagatgt aattaacaaa aatcctggta aaccagttgt aattttactt aatggctctg
    22861 taactatcgt ggtaattagc ttatttatat ccatattaaa caattgaatg attataaatg
    22921 acaataaagc aaacgaggta atcgtaataa tgattgggat taatacatta aaagacttta
    22981 aaacagcggg aggaatattt ccggatagct gaatctgtag tttcttatta ttagaaagtt
    23041 taataaataa ttctgtagcc gctaatccaa caaatatacc tacaaacatt cctgaagctc
    23101 ctgtatagga tataggtatc acacccgtca cttcaacagc tttatccgca ttttcaggag
    23161 taaacatcat ctgcataggt gttaatacaa caaatgtcga aatagcaact aataccgctg
    23221 caatatgatt tgtataattt ctgcttgcac acatatgata agatactgct gctacaacta
    23281 ataatgtagt tatgcttaat gtaccattgg caattgaagt accaatttgt tgtaacgtgg
    23341 tcaatgtact agtatcgaca atttccccca tgaatcctgt tggttctaag atcactgaat
    23401 taataagtgt cacaaatcct gctaacatca taaatggcat aaatgtagca aacgcatctc
    23461 gcaaagttct taaatggatc tgattcccca acttagtcgc aactatacca aatttttcaa
    23521 atacaatgtc cttcctgcta cttttgccca tattttttct cctttatgct tattgtaatc
    23581 gattactttt tccacccaaa taatacatca cttatcaaaa aaagtaaata cttttaatta
    23641 aatatatata tcttttttag tataaaaaaa taagctctga aaaaataatt ttcccgagct
    23701 tatttttcaa caaaaacagc ttcaaaaaca aagtcttgat ataaatgtct cgataccgaa
    23761 tactcgaaag gtttaccaga cgatagagca acaactttgt ctatctcaat cataaattca
    23821 cctggatcta tttttaatat ttttgcgtca aattcattcg cagtatctcc tttg
//
LOCUS       DASNNU010000038        23757 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.038, whole genome shotgun sequence.
ACCESSION   DASNNU010000038 DASNNU010000000
VERSION     DASNNU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.038"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(164..388)
                     /locus_tag="U8116_001427"
     CDS             complement(164..388)
                     /locus_tag="U8116_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HEN8434431.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="U8116_001428"
     CDS             complement(586..2166)
                     /locus_tag="U8116_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEN8434432.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="U8116_001429"
     CDS             complement(2567..3943)
                     /locus_tag="U8116_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN8434433.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="U8116_001430"
     CDS             complement(4071..5189)
                     /locus_tag="U8116_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN8434434.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="U8116_001431"
     CDS             5331..5765
                     /locus_tag="U8116_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8434435.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="U8116_001432"
     CDS             complement(5843..6376)
                     /locus_tag="U8116_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEN8434436.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="U8116_001433"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="U8116_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEN8434437.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="U8116_001434"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="U8116_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEN8434438.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="U8116_001435"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="U8116_001435"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN8434439.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="U8116_001436"
     CDS             complement(10101..10679)
                     /locus_tag="U8116_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEN8434440.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="U8116_001437"
     CDS             11001..11471
                     /locus_tag="U8116_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEN8434441.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="U8116_001438"
     CDS             complement(11579..12805)
                     /locus_tag="U8116_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN8434442.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="U8116_001439"
     CDS             complement(13559..13759)
                     /locus_tag="U8116_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN8434443.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="U8116_001440"
     CDS             complement(13893..14501)
                     /locus_tag="U8116_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEN8434444.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="U8116_001441"
     CDS             complement(14586..15056)
                     /locus_tag="U8116_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEN8434445.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="U8116_001442"
     CDS             complement(15104..16018)
                     /locus_tag="U8116_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN8434446.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="U8116_001443"
     CDS             16164..16967
                     /locus_tag="U8116_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEN8434447.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="U8116_001444"
     CDS             17145..18092
                     /locus_tag="U8116_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEN8434448.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="U8116_001445"
     CDS             complement(18160..18453)
                     /locus_tag="U8116_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEN8434449.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="U8116_001446"
     CDS             complement(18481..19146)
                     /locus_tag="U8116_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEN8434450.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="U8116_001447"
     CDS             complement(19286..19918)
                     /locus_tag="U8116_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN8434451.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /locus_tag="U8116_001448"
     CDS             complement(19925..20992)
                     /locus_tag="U8116_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN8434452.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /locus_tag="U8116_001449"
     CDS             complement(20989..21720)
                     /locus_tag="U8116_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEN8434453.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="U8116_001450"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="U8116_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEN8434454.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="U8116_001451"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="U8116_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEN8434455.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         7043 a         4828 c         3825 g         8061 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttattt
//
LOCUS       DASNNU010000039        23645 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.039, whole genome shotgun sequence.
ACCESSION   DASNNU010000039 DASNNU010000000
VERSION     DASNNU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.039"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="U8116_001452"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="U8116_001452"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEN8434456.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="U8116_001453"
     CDS             complement(1174..1869)
                     /locus_tag="U8116_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEN8434457.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="U8116_001454"
     CDS             complement(1882..2598)
                     /locus_tag="U8116_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEN8434458.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="U8116_001455"
     CDS             2928..3536
                     /locus_tag="U8116_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEN8434459.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="U8116_001456"
     CDS             3861..4184
                     /locus_tag="U8116_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEN8434460.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="U8116_001457"
     CDS             4366..5736
                     /locus_tag="U8116_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEN8434461.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="U8116_001458"
     CDS             complement(5866..7167)
                     /locus_tag="U8116_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN8434462.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9479)
                     /locus_tag="U8116_001459"
     CDS             complement(7521..9479)
                     /locus_tag="U8116_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEN8434463.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="U8116_001460"
     CDS             complement(9656..9922)
                     /locus_tag="U8116_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN8434464.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="U8116_001461"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="U8116_001461"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEN8434465.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="U8116_001462"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="U8116_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEN8434466.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /locus_tag="U8116_001463"
     CDS             13266..13829
                     /locus_tag="U8116_001463"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN8434467.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="U8116_001464"
     CDS             complement(14082..14603)
                     /locus_tag="U8116_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEN8434468.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="U8116_001465"
     CDS             complement(14606..15448)
                     /locus_tag="U8116_001465"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEN8434469.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="U8116_001466"
     CDS             complement(15441..16166)
                     /locus_tag="U8116_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEN8434470.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="U8116_001467"
     CDS             16342..16638
                     /locus_tag="U8116_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434471.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17206..18798
                     /locus_tag="U8116_001468"
     CDS             17206..18798
                     /locus_tag="U8116_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN8434472.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="U8116_001469"
     CDS             18811..19551
                     /locus_tag="U8116_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN8434473.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19719..21068
                     /locus_tag="U8116_001470"
     CDS             19719..21068
                     /locus_tag="U8116_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN8434474.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21196..21786)
                     /locus_tag="U8116_001471"
     CDS             complement(21196..21786)
                     /locus_tag="U8116_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN8434475.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21933..23285
                     /gene="brnQ"
                     /locus_tag="U8116_001472"
     CDS             21933..23285
                     /gene="brnQ"
                     /locus_tag="U8116_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN8434476.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6771 a         4762 c         4503 g         7609 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagtcatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaaata aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtatctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caatcaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacaaactt ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcca tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgaacaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaacagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgacttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatta ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt ctgaaaattt tggaatctta gttgtgtttt tcagaaaact cttttaatat
    19681 ggaagtaatt aaactttttt agttaggatg atttaaaaat gaatgaagag caagaactgc
    19741 agcgtggact gaaaaataga catgtccaac tgatttctat tggcggagcg attggaacgg
    19801 ggttgttttt aggttccgga aagtctatcc agctagctgg cccttcgata ttgctcgcct
    19861 atcttattac tggatgcatc tgttttttca ttatgcgtgc gttaggcgaa cttctgttat
    19921 caaacaccaa taaccattct tttctggatt ttgttgcaga atatcttggc aaaaaagcgg
    19981 ctttcatcac tggctggacg tattggttct gctgggtatc gattgcaatg gccgatttaa
    20041 cagccatcgg aatgtatgta cgctactggg cgcctggtgt tcctcagtgg cttcctgaac
    20101 tgattgcatt aggtcttctt ctttgcttga atatggtggc tgtctcattg tttggtgagt
    20161 tagagttttg gtttgcattg atcaaagttg tagcgatcat tgcctttatc ctcgtaggag
    20221 cttatatgat cctcacccac tatacgacag atattggcac agccagtatc acgaatctat
    20281 ggtctcatgg cggattcttt ccaactggtg caaaaggatt tatcttagct ttccaaatgg
    20341 taacctttgc ttttgctggg atagaattag ttggattagt cgctggagaa acagaaaatc
    20401 ctgaaaaagt gttgccggaa gctatcaata atattcctat caggatcatc ttattttact
    20461 taggatctct ttttgtcatt atggcaatct atccatggaa cagcttgaat ccagataaca
    20521 gtccatttgt agaagtattt tcggaaattg ggatcacgat tgctgcttcc ttgatcaatt
    20581 tagtcgtatt aagtgcagca gcttctgcct gcaacagtgc aatatatagt acaggacgta
    20641 tgcttcgttc tttagcgcaa gaaggcagtg cccctaaaaa attcaaaaag ttaacaacac
    20701 atcatgtgcc agggaatgct ttgacatttt ctactatcgt cattttcatt tctgttatct
    20761 tgaattacgt gatgcctagt gaagtattca cactagtaag cagtattgcc actacttgtt
    20821 tcttgtttat ctggagtatg cttgtctaca cgcacatgaa atatcgtaaa agtatcctcg
    20881 gcaaaaaagc tcattcattt aaaatgccgt tgtacccatt tagtaattat ctggtatttc
    20941 tttacatggc ctttgtgtgt gtcgtcttgt tcctaggaaa agatacgaga atcgctttat
    21001 tactaacacc tgtgtggttc ttgcttctat tactaatcta tcatatgaaa tatgaacgta
    21061 agaagtaaga aagtagttgt gacagaagtg gtcagcttcg agaaataaga aggaatttac
    21121 gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt tcttggagtt aaaagcttct
    21181 gtcccagcct cttttctatt tccccggaaa caaccgctga taataggcat ctgctttttt
    21241 ataggaaaaa atccaaatac cagatacaaa aaaacgtccg aataacccga tcaacggtac
    21301 aaaaaagaca ccggcaatca agcataagtt aatcatcatg gagataagac ttcgacggtc
    21361 ctttgacaaa actctcacga catgggaatc tggtttattt gctttcatga tttgcctcat
    21421 caagatacca aaagaaaaac tccacatcgt gtatactaag acataagcca tctcaggcca
    21481 aaaagcttca gggttacgcc cgacccaact agtaacagaa ggacataaag tcagccaaaa
    21541 gagcaaaaag atattcgacc agaccactcg gtaattaatt cgttctgcca tctgaaatat
    21601 acgatgatgg ttcacccaga tcaccgcaac aaatataaag ctgatggtat aagctaataa
    21661 tgtatttcgg atgacccata aagaatgaaa gctagatgta ttagggatct tgatctctaa
    21721 tacaagaact ctcaaaacaa tggctaccac tccatcagta aatgcttcta atctcgactt
    21781 tgtcacgatg attcctcctc agatgattca gcgcttttat tttatcactt acgttattaa
    21841 ataaccgtcg ttttatatag gaaattttct gaaaattgtt gcatattttt cgaaattttg
    21901 ttacttttaa aaaaaccgta aaggagttat gtatgacaaa aaaattatca tttaaagact
    21961 atctttttat aggctccatg cttttcggtc tattttttgg tgctggaaat cttatctttc
    22021 ccgttcattt gggtcaagaa gcaggcgctg ctaccttttg ggcgaatcta ggtttcttgg
    22081 ttaccggtat cggtcttccc ttcttagggg tgattgccat tggtgtatcg aaaagctcag
    22141 gtgtatatga attagcaaaa cggataaatc aaacatatgc gatggttttt accatcttgc
    22201 tttacttagt tatcggaccg ttttttgctt tacctcgcct tgcaaccaca tctttcgaga
    22261 ttggtttagc accatttatc gatcctagca gacagacgct atatctagca attttcagta
    22321 ttgtattttt tgtgctggct tggtggtttt caagaaaacc gacaaagata ttagattatg
    22381 ttggaaagtt cctgaatcct gcatttttgg ttttattagg tgctttgctt ctccttgctt
    22441 ttttgaatcc actaggttct gtaaatcaag cgcctattca gccaaattat caagagcatg
    22501 cttttttcac agggtttact caaggatata acacattaga tgcgcttgct gctttagcat
    22561 ttgggatcat cattgtcaca acaatccaaa acatgggtgt aacaaaacct gctgaaatcg
    22621 ctaaagacac gatcaaatct ggtgcgatca gcattatatt gatgggaatc atttatactt
    22681 tgctcgctta tttaggtgct atgagcttag gtagctttgc tctaagtgaa aatggcggga
    22741 tcacgctggc tcagattgct gatcattatt taggtactta tggcagtatc ttattagcat
    22801 ttatcgttat attagcttgc ttgaagaccg gtattggttt gattacagct tttgcagaaa
    22861 cattcacaga tttgtttccg caaagaaatt acgcattctt tgttgcactt gctagtatcc
    22921 ttccttgttt agcggcaaac gtaggcttga cgaatatcat tcaattttcg ttgcctgttt
    22981 tgatgttcat ctatccatta gcaatgacac tgattttact agtgttagtt ggtcctttgt
    23041 ttaagcagcg tacggctgtt tatcgaatga ctacttattt tacattgatt gcttcgattt
    23101 ttgatgggtt gaacgcttgc ccagaaacta tcaaacaaac acctattgtt caaaatcttt
    23161 tatatgttgc ggaaagctac ttgccgtttt tcaaattggg gatggggtgg atcgtaccag
    23221 cagttatcgg ttttgttatc ggactgattt ggagtttcgc taagaaagaa gaagtagcag
    23281 attgataaaa gaggttgtga aagagacgtt cagccttatt gccgataggc tgtctcttga
    23341 acactgttta ttgagaggtc gtgaaaaagc cgctcagttt cgagtattaa gaaatatttt
    23401 ttgaaaatgg cttctcacat ttttgcataa atttgactta ataccgagaa actggctttt
    23461 gaacaccgtt tattgagcgt ctataaactg acctgagtat tagagaaaca aaacggcaaa
    23521 gtagctccat atactttgcc gttttgtttt gaactaatac cgaagagaag ctgatcaaac
    23581 aacatttctt ttataatata gattttagac atatatatga tataaaaata gttttcgcaa
    23641 aaaaa
//
LOCUS       DASNNU010000040        22906 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.040, whole genome shotgun sequence.
ACCESSION   DASNNU010000040 DASNNU010000000
VERSION     DASNNU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.040"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(104..1336)
                     /locus_tag="U8116_001473"
     CDS             complement(104..1336)
                     /locus_tag="U8116_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEN8434477.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="U8116_001474"
     CDS             1591..2160
                     /locus_tag="U8116_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN8434478.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="U8116_001475"
     CDS             complement(2338..2778)
                     /locus_tag="U8116_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEN8434479.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="U8116_001476"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="U8116_001476"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEN8434480.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="U8116_001477"
     CDS             3732..4655
                     /locus_tag="U8116_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEN8434481.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="U8116_001478"
     CDS             4731..5870
                     /locus_tag="U8116_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEN8434482.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="U8116_001479"
     CDS             5863..6663
                     /locus_tag="U8116_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN8434483.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="U8116_001480"
     CDS             6663..7490
                     /locus_tag="U8116_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN8434484.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="U8116_001481"
     CDS             7468..8202
                     /locus_tag="U8116_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN8434485.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="U8116_001482"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="U8116_001482"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEN8434486.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="U8116_001483"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="U8116_001483"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEN8434487.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="U8116_001484"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="U8116_001484"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEN8434488.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="U8116_001485"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="U8116_001485"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEN8434489.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="U8116_001486"
     CDS             11787..13820
                     /locus_tag="U8116_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434490.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="U8116_001487"
     CDS             13864..15144
                     /locus_tag="U8116_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEN8434491.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="U8116_001488"
     CDS             15354..16160
                     /locus_tag="U8116_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8434492.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="U8116_001489"
     CDS             16172..17392
                     /locus_tag="U8116_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8434493.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="U8116_001490"
     CDS             17382..18968
                     /locus_tag="U8116_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN8434494.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="U8116_001491"
     CDS             19007..21145
                     /locus_tag="U8116_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN8434495.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="U8116_001492"
     CDS             complement(21513..22496)
                     /locus_tag="U8116_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEN8434496.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7195 a         3903 c         4726 g         7082 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacga
//
LOCUS       DASNNU010000041        22716 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.041, whole genome shotgun sequence.
ACCESSION   DASNNU010000041 DASNNU010000000
VERSION     DASNNU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.041"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(8..1939)
                     /locus_tag="U8116_001493"
     CDS             complement(8..1939)
                     /locus_tag="U8116_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN8434497.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1957..2871)
                     /gene="pfkB"
                     /locus_tag="U8116_001494"
     CDS             complement(1957..2871)
                     /gene="pfkB"
                     /locus_tag="U8116_001494"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEN8434498.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2868..3620)
                     /locus_tag="U8116_001495"
     CDS             complement(2868..3620)
                     /locus_tag="U8116_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN8434499.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3779..3988)
                     /locus_tag="U8116_001496"
     CDS             complement(3779..3988)
                     /locus_tag="U8116_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434500.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(3999..5327)
                     /locus_tag="U8116_001497"
     CDS             complement(3999..5327)
                     /locus_tag="U8116_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434501.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5463..5618
                     /locus_tag="U8116_001498"
     CDS             5463..5618
                     /locus_tag="U8116_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434502.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5692..6279)
                     /locus_tag="U8116_001499"
     CDS             complement(5692..6279)
                     /locus_tag="U8116_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEN8434503.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6362..7612)
                     /gene="clpX"
                     /locus_tag="U8116_001500"
     CDS             complement(6362..7612)
                     /gene="clpX"
                     /locus_tag="U8116_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEN8434504.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7859..9148)
                     /locus_tag="U8116_001501"
     CDS             complement(7859..9148)
                     /locus_tag="U8116_001501"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEN8434505.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9302..10234)
                     /locus_tag="U8116_001502"
     CDS             complement(9302..10234)
                     /locus_tag="U8116_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434506.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10391..11050)
                     /locus_tag="U8116_001503"
     CDS             complement(10391..11050)
                     /locus_tag="U8116_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN8434507.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11053..12078)
                     /gene="sppA"
                     /locus_tag="U8116_001504"
     CDS             complement(11053..12078)
                     /gene="sppA"
                     /locus_tag="U8116_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN8434508.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12091..12381)
                     /locus_tag="U8116_001505"
     CDS             complement(12091..12381)
                     /locus_tag="U8116_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434509.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12790..14595
                     /gene="glmS"
                     /locus_tag="U8116_001506"
     CDS             12790..14595
                     /gene="glmS"
                     /locus_tag="U8116_001506"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEN8434510.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14667..16022)
                     /locus_tag="U8116_001507"
     CDS             complement(14667..16022)
                     /locus_tag="U8116_001507"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEN8434511.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16046..17215)
                     /locus_tag="U8116_001508"
     CDS             complement(16046..17215)
                     /locus_tag="U8116_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434512.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17212..18099)
                     /locus_tag="U8116_001509"
     CDS             complement(17212..18099)
                     /locus_tag="U8116_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEN8434513.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18288..21980)
                     /gene="nifJ"
                     /locus_tag="U8116_001510"
     CDS             complement(18288..21980)
                     /gene="nifJ"
                     /locus_tag="U8116_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEN8434514.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22179..22700
                     /locus_tag="U8116_001511"
     CDS             22179..22700
                     /locus_tag="U8116_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN8434515.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6326 a         4932 c         4041 g         7417 t
ORIGIN      
        1 tttatttcta tgcttctgct actgcaggag cttttctcaa taaaccataa atcacaccag
       61 atacgactgc tccgatagca ataaacacca aatacatgac cgggtgactc agtaagaata
      121 caacgaaaat cccgccgtga ggagccagta atttgattcc gaaagctccg actaatccgc
      181 cagttaaagc agaaccaata ataaaacttg gaatggcacg aatcgggtca gctgctgcaa
      241 atggaatcga tccttccgtg acaaacgata gtcccatcac gatgttcgtc aaaccagcat
      301 cttgatctgt tttgctgaat ttattcttaa acaaacgtgt agcaacgaaa attgccaatg
      361 gaggtaccat tcctccagcc ataacagaag ccatcacgac acttccgcca gttgcaacag
      421 tcgtagctaa agttgctgta ccaaacacat aagcggcttt gttgattgga ccgcctaaat
      481 ctgccgccat cattccagct aataatgcac caagtaaagc agcattcgaa ccactcatac
      541 ttgtcaagaa accgttcaat gcatcattga ttgctttcat agggacattg accagcaaca
      601 tcaagaaacc agtaatcagt aagccaaaca ctggataaaa gaggatcgtt ttaattccat
      661 ccaatgattt tggtaagttc ttgaataatt tacgtaaaaa tacgatgaca taaccagcta
      721 agaatccacc aatcagtgca cctaagaatc ctgcaccgcc tgtattagct aatgcaccag
      781 cagcaaatcc aacgatcaaa ccagggcggt caccgatact tgaagcaata aatcctgcta
      841 ataccggcag catgaaacca aatgcagctt gtccgatttg gttgaaccag ctagcagctt
      901 gattgtaatt ccctagattt gataattgat catgtggcac gccaataaat tggtcgatca
      961 tgaaagaaag cgcaatagcg attcctccac caataacgaa aggtaacatg tgtgatacgc
     1021 cgttcatcaa atctttgtaa attctttgtc cgatagtacc atccgcattt ccttcttctg
     1081 aactgctttc ttttccatca ccatggaata ctggtgcagt accactaagg gctagattga
     1141 tcaactcttc tggtttacgg ataccatcac tgacaggacg attgatcagt tcttttccgt
     1201 caaagcgatt catctctact tttttgtctg ctgcaacgat tacaccttct gcacgggcga
     1261 tgtcttcagc agtcaatcgg tttttgattc cttctgaacc gttcgtttcg actttgatct
     1321 ccacacccat ttctttcgct tttttcttca aagcatcttc tgccatgtaa gtgtgagcga
     1381 tccctgttgg acaagctgta acggcaacaa cgaattttct ttctggatga gcagaagctt
     1441 ctgcagcttg tgtttcttgt tctgcttttt cttctgcttc tttttgctct tcagctgttt
     1501 ggaacaagtc ttggacttct tctggtgtat ttgcttcttt taattttcct acgaaatctg
     1561 catcaatcag caatcgagat agtgctgcta atgcttgcaa atgtgtgtcg tttgcgcctt
     1621 ctggagctgc gatcatgaaa aacagataag ttggctgtcc gtctaaggct tcataatcta
     1681 ctcctttttg gctttttgcg aataaaactg ttgcttcttt aactgcactg tttttcgcgt
     1741 gaggcatggc gattccatca ccaagacctg tagaagtttg tgcttcgcgg gctagaattc
     1801 cttctttata tgtttcgata tcggaaatcc ggccggcatc aaacattttt tggaccattt
     1861 catcgattgc gcctttttta tcagtcgctt gcaaatccat gatcatcgct tctttgacca
     1921 ttaaatcctt gatattcatg attatcctcc ttgaaactaa tcaatttttg tgatttcaac
     1981 ttctggcagc aactcatcaa tcaattcgcg agctgccaag tcatcagaaa aagctgttgc
     2041 gcttccgcaa gcaactcccc atttaaaggc ttcgattgga tctttcgtac ggctgtaagc
     2101 cccaataaag ccagcaatca ttgaatcccc tgcacctact gaatttttca atgggcgaac
     2161 taatacattc gttttataag ttccttgttc cgtgaagagc aaagcaccgt ctccagccat
     2221 tgagatcagc acattctgtg ccccttcagc tagtaatttt ttcccatacg gaatgatttc
     2281 ttctacagat tggaaggttg tgttgtaaag ctcagctaat tcgtgattgt ttggtttgat
     2341 caacaacggt ttcttaacaa gtgcattttt taggtcatca cctgttgtat caatcacgaa
     2401 atctgcacct ttttctcgaa tgatatcaat cagttcttga taaaatcctt ttcgcaggct
     2461 agctggcgcg cttcctgaca atacgacgat atctccttgt cctactgttc ctaatgcttg
     2521 cttcaactca tcgatttctt catctgaaat agatgggcct aagccattga tctcagtttc
     2581 agtgtctgat tttagtttga tattgattct tgtatcttct tttacggttg tgaacaaaga
     2641 ctggatttgt tctttttcaa gccacttcga aataaattcg ccagtgaacc ctcctaaaaa
     2701 ccctagtgct gtagattgag aatcgatccg tttcaaaata cgggaaacat tgatcccttt
     2761 tcctccaggt aacttgaagt cttccttcat tcggttcaaa tcgcctagtt ttaacccatc
     2821 tacgcgtacg atataatcaa ttgatggatt aagtgttact gtgtaaatca ttgtattgcc
     2881 tccttaattg ttgtcttttc acggaattca ttaacgaatt ctaatggaca acgctcggtt
     2941 atgatcgtgg ctgcttccaa aggtgcaaca gaggtgaagg acaactggtt gaatttggta
     3001 tgatcaatca atacaaatgt ctcttcggaa tttttcatgg ctaaacgttt aacagctgcc
     3061 tcttctggat ccggagtcgt gaatccttgt tcaagatggg caccgttcat tcccataaat
     3121 gctttattaa aatgaaaatt ttttagttgt tctatcgcat tagatcctaa aacagcatta
     3181 gttgccagct ttatcgttcc acctaaaata atggtagcaa gctccaaatc aactaaattt
     3241 gccgcatgtt gaacggaatt tgtcactact gtgatctgtt ttccttgcag aaaaggaatc
     3301 atttctaacg tagtagagcc agcgtctaaa taaatgacat cttcgtcatt aatggtttca
     3361 gctgccgatt tggcgatttg ctgtttttcg tgaacgtttt tgagtgattt ttcactcata
     3421 ttctgctcaa aattcaataa ttgctctttc ttagctccgc catgaactct ttctagaaac
     3481 ccactattct ccagctcttg taaatcacgt cttatcgttg attcagaagc attcaaaagg
     3541 tttacaagat cttgtgattt cacgattttc tgctgttcaa gcaagtctag gattttttgt
     3601 ctgcgttcct ctgtaagcat tttcacgatc acctcaatac aaataatagc atattttttt
     3661 tctactagca atataaatca gtcaaaaacg ttcataaaaa ttataaatca gtcaaaaaaa
     3721 aataaaaaac cagtcagaaa gactggtttt ttattttttt gtctatcatc aagccgtttt
     3781 atcgtttttt attttcgctt cttgtttttt tcatataaac agaagcaggt tttctttcct
     3841 tatgaagaag ataataataa agtcccatcc cgccaaacat gaccaaaaaa ccgatgactg
     3901 atcgaatagg aaaattagga acaaaaaaga gacagacgga taataatgat gccggaacca
     3961 ttattcgata atagagttgt ttatccattt tcttcacctc atattttttg taaagtaatc
     4021 aattgttcta attctcggta atctaaaaat tgcacacgca gatgctgctc acctttcttg
     4081 acatcgaatt ggatacttcg ccgctttttc tttattcgtt tgaaggtaac atcagagagt
     4141 ctatgtttga tttcctttaa cggtaattct gacagggatt ctgaaggaac atagattctt
     4201 aaggataaaa gatactcttg atatttttca tctgttacct ctacttctag atattcccta
     4261 atataataac aataagcttc gattccatta tccaaagcaa cagtcggatc gataacttta
     4321 tagcacatca tgcgtgatcg attctcgtca acccaaggaa aatacgcctg agcatttagt
     4381 gttccgcgaa cacgcctttc tttataatct ccttgttgga tatcataact ttttgttaca
     4441 aaaaatgaag cattcttttt aggaacaggt tgcatttgtg cattctgata ctggacagat
     4501 tggatcgtat ggtcagtgta aatgtattca acgaatggcg gtttcaataa accgacacca
     4561 ttgtcccaag caagatgatc aaacccaagc tggtccctaa aataggattg aaacatttga
     4621 ttgtttggct tttgccagtt aaaggggtct cgataaagct ggatacatat gacttcgttt
     4681 tctaattcct tcactttttc tacttgttgg atgtcgagaa aaatcggttt agggatgatt
     4741 tgatgataat agcgactata atacatttcc caacacgaag tgaaacataa accttggtaa
     4801 aaaagatcgt atcctggaaa ttggttgcaa tcgactacta cttccccgtt ttgttgtttc
     4861 attttaggta aatgttcgat ttcatccttt ttctcaaaca atagccgttg agagatctgt
     4921 tttgtattgt gatagataaa ctcgctataa gctcgcatat aggcgaacac cccatgagca
     4981 atcatttttt tctgaatgaa gtctcgaatc aaaaattgct tttctttgaa aacagagata
     5041 gacaatacat tttttatcaa taattgatta tcttccaagc gaaaactaca ttcgttctcg
     5101 tcatttccta caactaacca actttcgtct cttaccgatg ctagcattat tttttcttga
     5161 agctttcgaa gatcatacgg atccgcttgt tgtgtgaaag agcggataaa tgttggctgg
     5221 atcaacagac gtgtcaaaat agcccaatcg cttatttcct gttttttttc ttcttcattt
     5281 aagtctgtga gctggatagt catcaataat ttatcttcgc caatcatttt ctcacctctt
     5341 tcaaatcttt ttataattta attataacca atgataaaga aaaactaaaa ctttatgtct
     5401 tagaaaccaa tttggacgat tacatgatag actaagtaaa aagaaacaaa gaggtgttta
     5461 caatggggtt gaaatttgaa cgtacagata atttagataa acttttcgaa tcatttgctg
     5521 tcgatccgga aaaaacagtt gcaccaaaag aagagatcga tcgatttcta aaaccacagc
     5581 cacaaaatga aaagaaaaaa gacaagaaac aaaagtaagt taagctcaaa aaaagactta
     5641 ccagccaaac ttttatgaaa gagtttgact ggtaagtctt ttttattctt tttatgattc
     5701 aattgcttgt tcgattgctg cccacgcttc ttcttttccc tgttttgtaa cggaagaaaa
     5761 gagaataaac gtgtcattgc gatcaaaatc caacgctttt ttgatttcgc tctcatgctt
     5821 gttccattta ccacgaggga tcttatctgc cttagtggca acaacaatta cagggatatc
     5881 ataatacttc aaaaattgat acatctggat atcttcttga gaaggcttat gacgcaaatc
     5941 tactaaagaa acgaccgcac gtagctggtc tctagtcgtt aaatatgtct cgatcatctt
     6001 tccccatttt gctcgctcag ttttggatac ttttgcatac ccatatcccg gaacatcgac
     6061 aaaatgtaat gcgttctcga tcaaataaaa attcaatgtc tgggtctttc ctggcttacc
     6121 ggaagtacgg gctaaattct ttcgatcaat caatgtgttg ataaaagaag atttccctac
     6181 atttgatcgt cctgccagag ctatttctgg caattgtgta tctggatatt gttttggtgc
     6241 cactgcactt atgataattt cagcttgatg gactttcatg gtctctctcc ttaacaaggt
     6301 gatgaataaa gcaagcggct gtcgcaaaag ccaaaatgac ttttacggca gcctcttttc
     6361 atcaacccgc ttttttctct gaatctttat agattactgt tggctcacca gacaattcag
     6421 ctgcttcttt tgtgataatc actttttcga tgttttcatt tgaagggata tcaaacatca
     6481 catccatcat gatttcttca atgatggaac gaagtcctcg agcaccggta ttgcgttcga
     6541 ttgctttttt cgcaatggct ctcaacgctt caggttcgaa ttcaagttcg gtattatcta
     6601 gagaaagaag tttttgatat tgtttgacta gcgcattttt cggctcagtc aagatccgta
     6661 ccagatcatc tgttgtcaat ttctctaaag ctgccataac aggtaatcgt ccgataaact
     6721 cagggatcaa tccgaatttt agtaaatctt caggaatgat atgctgcatg acactttcgt
     6781 cttcagaaag tttttggttt tgtgtaccaa atccaatcgt cttttcgccc atacgattct
     6841 tcacaatcgt ttcgatgcca tcaaatgcac cacctacgat gaacaatatg ttcgtcgtat
     6901 caatctgaat aaattcttga tgtggatgtt tacgtccgcc ctgaggtggc acacttgcaa
     6961 cagttccttc tagaattttc agcaaggctt gttggacacc ttcgccggaa acgtcacgag
     7021 taatagatac attttcgctt ttacgcgcaa ttttatcgat ctcatcaata tagatgatcc
     7081 ctttttctgc aagttccaca ttgtaatcag cagattgaag caatttaagc aaaatgtttt
     7141 ctacatcttc ccccacataa ccagcttctg tcaaacttgt cgcatctgcg attgcaaaag
     7201 gtacattcaa tgtttttgct aatgtttgtg ctaaaaatgt tttaccagac cctgtaggtc
     7261 cgatcaagca gatattgctt ttttgcagtt caacatcatc tgtgttttct tgtgcttgct
     7321 gattgacacg tttatagtga ttgtatacgg caacagctaa cgagcgtttt gcgcgatctt
     7381 gtccaatcac gtagttattc aatacttcaa gaatttctag tggtttaggg acttcagtga
     7441 attcgcgtac agcttcttca taaaattctt catcaatgat ttctttacat aggtcgatac
     7501 attcattaca gatgtagacc cctggaccag ctacgatctt tttcacttct tcttgggttt
     7561 tcccgcaaaa agaacagcgt acggtctcat tgctacttgg attgtcatac atggtcttca
     7621 ccccttaact tgcaaaaagt atgcaagaaa ctatcaaaga ctttacagtc ttttactatc
     7681 ataacatagt ttagtaaaaa gaaaaagtgt ttaacaaagg atcaactgac ctataccttt
     7741 gcatttttta ctcttaacaa aaaataagga actgtgacaa aatcgtgtca cagtccctta
     7801 tttcgaataa acaattttct agaagtagct tcttcagaaa tgatttatta cttgatttct
     7861 attctttcgc ttctgtcgct gtacctgtga tcaattcgat tgcttttttc attgtcacat
     7921 catgttccag catatcttct gtcaacacgc gtttgatttg ttcgattggc atgttgtatg
     7981 tttctgataa ttcttggatt tctgcattga tttcttcttc tgttgcttga agattttcag
     8041 cagctgcaat tgcttcgatc acaagatttg ttcttgtgcg catttctgct tcaccttcga
     8101 attgtttatg caagtcttct tctgtagaac cagttaattg atagtacatt tcaggagaga
     8161 taccttgacg ttgcatgttg ttcaagaatt cgtccattga acgatgtact tcatcatgaa
     8221 ccatcacgtg tggcaattca acgatttctg cgttttcaac agccatacgg atcgctgctt
     8281 catcttttgc atcgtccgct gctttttctt ttgtttctac taattctttg cgatattttt
     8341 ctttcaattc gtctaatgtt gatacttcgt catcaacatc ttttgcgaat tcgtcatcta
     8401 attcaggcac ttcttttgct tttacttcat ggattgtcac tttgaagact gcttcttttc
     8461 cagcaagatc ttctgcttga taatcttctg ggaaagttac atttacatct agactttcac
     8521 ctgatttatg accaactaat tgatcttcaa aacctggaat gaatgagcct gaacctaatt
     8581 caagtgagta attttcgcct tttccgccat caaacgcttc gccatctaca aagccttcga
     8641 agtcgattac tactgtatcg ccattttcag ctgcagcatc ttctttgata actaattcag
     8701 cttgtgcttc ttgttcacgt ttgatacgtg catccacgtc agcatctgtt acttcacggt
     8761 cttgtttttc aacaactaga tctttatatt gtcctaattt tacttctggt ttcactgtta
     8821 cttcagcagt gattacccag tcttgacctt tttccatgct ttcaacatcg atttttggtt
     8881 gagaaacagg atcgatgcca gcttctttga cagcagcttc ataagcttct ggtaatacag
     8941 cattcaatgc atcttcatat aaagcttctt caccgtacat acggttgaat acttgacgag
     9001 aaactttccc tttacggaat ccaggtacgt tcaagctttt tttcacttta ttaaacgcag
     9061 ttgttaatcc tttttggatt tcgtcttgcg caatcgtaaa ttttaaaaca ccatcattcg
     9121 tgcctttttt ttcaaatgtt gcagtcattt aattccctcc gagtttagaa attcatttta
     9181 tacatcaaaa acgtttcatg cataccttta aatagtacac taaccctttc aaattgtaaa
     9241 catattgaat ggatagaatc cttgtttttc ataacatttt tatctttttc actgataaaa
     9301 tctaatggaa gaagatcatt tgctgcaaaa ttttttctct tttttttaaa aaatcttgtt
     9361 tttcatcttc tgtaccttca tacatcgttt ttcctgtaca cgcctctaca gtcagttgaa
     9421 accagcgctt cgggtcttcg atgtatgtat catagacagg ataaacaatt gcagattgca
     9481 acgtgaattc ttcttttaac acggaactaa gcagcgcatc ttcattcccg taacgatctg
     9541 aaaatacaca taattgctgg taacttgcct gctgctgcgg ctttggcaga tcttttggta
     9601 tgactttgac taacttttct tcaatcgtca aatagaaaac ttgatggcat tcgttcaatt
     9661 gcctaagtgt ctccagtaat tttgcccttg ctaaaatatg gacctctggc gatacaataa
     9721 gtttactcgc tgtttggata aactcatgat aaggaagctg ttcgatctca tcaatagacg
     9781 caagctgacg tgcaggttcc aacgaaggaa gttcctccga tttattgtgg agtgccttga
     9841 tcttctgcct ctccatttgt ccggataatt cttctgcaaa ttggattttt tcaatgaccc
     9901 gctgctcgaa agcttcataa aaatctgtac ttgctatcaa ttttctagca tataaaaaat
     9961 cttgtgtact gattaacaag tcaaaataaa attcagcaaa ctcctcattt tgaagatact
    10021 gtttttcatg caagagtgct tgcttcaatg cttctttttt ctcgtccaac tcgtaaagac
    10081 aaaaaacaag caggctatta gcctcaaagc tttctttcaa ctcgtaagct cgttgaaata
    10141 attcttttgc ctgctgatat tgctggtttg ctagtgcttg ttgtgccact cgcattaaat
    10201 tctcatattt ttctggaaaa tcaatcggct tcacgcagtg tcctcctctt gctcatattc
    10261 agctcatgtt caatagtagc aaaacaaaaa aagcatgaca attaaaaata actgaagcac
    10321 cctctaaaag ctagatttta gatctgactt ttagaaggtg ctttcaactg ttactgcttt
    10381 ttattgctca tcagctgttg acttcctggt tgaacgcttt gatcagattg atcgtaacaa
    10441 cacttgtatt agtgaaaaaa tcgcccagat gttgcttttt ctgattaaag actgctggaa
    10501 gataccctaa catcagcaaa ggaaatttta gaatgaaccg gcaagcacct tctctaacta
    10561 aaacggttga ccagctaagt tcttcttcat caaagctgat gactcgaata ccaaagatca
    10621 ttttcccaat cgtttggcca tgattgaatt tcgtcaataa aataaaatac gctagataaa
    10681 taagtaaggc tgtgaatccg tagagactca gataagaatc tgaaaaatta agccaaccta
    10741 gatttttggc aataccaagt gttaaacgcg taatagctcc gatacacaaa agatcgacca
    10801 agaaggcaaa cattcggata aaaaaacctg catagaaata cttaggaaaa tcatttgctt
    10861 ttggcggttc atttcttgtg tccgtatact ttttccattt acgttgtttc tttttgattt
    10921 cttcgggcgt caaaggctct ctttcaaaag agctcagtac cttttgggaa aagttggagt
    10981 caggtcctac agcaggctgc tgttgcttat ctttttcttg ttccttattt tcatttgaaa
    11041 gctctgacat gcttactcac ctccgtaata atacattgct tttggtgatt ccgctgtccc
    11101 tagattctca agaacggata agatctgact agtctcagaa ggcttcaatc cctggaattc
    11161 tgctagttta cttcctaacc atgtactagc aaacccagat ggcgtggaag aatattctac
    11221 tagttccgca tcttcaagct gttgttcctt tcgtatagct tttaaagcat cttcaggaaa
    11281 tcccaattca tcgactaaac ctacttcttt tgcttggaca ccatcataaa tccttccatc
    11341 agcaattttt ttgacttctt cttcagattt attccgtccc tcgctgacga ttgctacaaa
    11401 acgactatat gcgctgtcga tatatgcttg aagcaccgct tcttcttctt ttgtctctgg
    11461 tcgcgttcct gaacccatat ctttcaacgc tccgctttta actgttgtat cttccacacc
    11521 cagtttttct agaagtcctg aataattcaa tcctgacatg atcacaccaa ttgagccggt
    11581 cactgtttct tctgttgcaa agattttatc tgcttgagca gaaatatagt agccaccgct
    11641 agctgccatg tttttcatgc tgacatacat tgggatatca tgttctttac gaatcgtatc
    11701 caatttctta gcgatttccg cactttcgta gacaccgcca ccaggagaat ttacttctaa
    11761 aaagatacct ttgactgttg gatcttcttc aatcgccttc agttgttcca taaaattttt
    11821 atgattgtaa ccttcagaag caaataaacc tgaagaccct cccgcagcaa tcgttccatc
    11881 tactgataat tttacgattt tttgcgtact gtctccctct tccaacacat ttggagctag
    11941 ttcttcatcc ccatataacc aactgttcaa tccctcaatt tgatcgtttt cctgaggctt
    12001 agtcaaacta gctgaaacta acgaaaccac aaataatcct gcagcaatca gaactgctgc
    12061 ccaacgtctt ttattcattc tttctccttt ctaatacaag ttttttatga cgatcacagt
    12121 ttctgactgc tcagaagtgt cgaaccgctc tgctttttct tcccgcgcat acccggaaaa
    12181 cgactctttt gttaattcat cttctaccgt aaactcacga gcttgagcaa cttccaaggc
    12241 atcaggtgcg atttcttgga tgctatccca gccagtatct aaaaatattt tcgtatcatc
    12301 tgatacacca gctgatgctt cgaatttgga caattgtttt tttaacattc ccagtgtcat
    12361 gatctcaatt tttgaatcca tttttactcc tcctctatcc gattactttc tattgtaccg
    12421 aactcctgaa aaagatgata gataaaaacc cattattttt tgacataaaa aggacatttg
    12481 acaaagaatt tgatatatac caatttattt tttattcttt ttttgttgac aagaacattc
    12541 gcaaaaggtt atgattttaa atgtttccag ataaaaaaca aaaaggagga tagttatacc
    12601 ttttaggata gcgccaggtc tgtgtaaaca gatgacgagg agagggttta tcgaaagatt
    12661 cggcggatgg ccctagggac tgcactctac agaaaatcca caaaaaatgt ctgcaaagat
    12721 aaaacaaaag gattttcctg cagctggaag cattcatacg aattcattat agaaagtagg
    12781 atgtttttta tgtgcggaat tgttggaatg attggattaa aaaatgtaac accaggattg
    12841 attgacggtc tagaaaaatt agaataccgt ggttatgatt cagctggtat ctttgtctca
    12901 gatggcacaa ctgattatct ggtcaaagca caaggcagaa ttcaaaattt gaaaaacaaa
    12961 ataaatattg atactacagg aaatataggt attggtcata cacgatgggc aactcacggg
    13021 caaccttctg aagaaaacgc ccatccacat acttcccaaa gtggacgctt cgtgctcgtt
    13081 cacaacggcg taatcgaaaa ctttgaagaa cttaaaattg cttatttagc aaatgaccac
    13141 tttattggtg agaccgatac tgagattatc gctcatctaa ttgaaacttt cgcgaaggat
    13201 actacaacgc aagaggcgtt tttaaaagct ttgcgggtca tcaaaggttc ttacgctttc
    13261 gctttgattg atcggacagc acctgatgtt atttacgtcg ctaaaaataa aagtccacta
    13321 ttaatcggac tcggcgatgg attcaatgtg attgccagcg atgcaatggc tatgcttgct
    13381 catacgaagg aattcgttga gattgaagat gaagagatgg ttaccgtcac atcagaaaaa
    13441 gttattattc aaaattttgc aggtgacatc gtagaacgtt cttcattcga agcacaagta
    13501 gatgcttcag atatcgagaa aggtacctat cctttttata tgctgaaaga aatcgacgaa
    13561 cagcctatta tcatgcgtcg tatcgctcaa aaatacgtaa cagaagacaa tcgtgtctct
    13621 cttgatcaaa agctggtcga tacattgtct gatagcgatc gaatctatat cgttgcttgc
    13681 ggaactagtt accatgcggg gttagcaggt aaacagactt tggaaaaatt gacacagatt
    13741 cctgttgagg ttcatttagc cagtgaattt ggctataaca cacctcttct ttcaaaaaaa
    13801 ccattcttta ttttcttgag tcaaagtggc gaaacagcag acagccgcca agtgctggta
    13861 aaaatcaacc gtctcggcta cccttcactt acgattacaa atgttgctgg ttctactctt
    13921 tcgcgggaag catcctttac actgctattg catgctggac ccgaaatcgc agtagcatca
    13981 acgaaagctt atactgcaca aatagctgta ctaactttat tagccaaagc tatcggtgat
    14041 aaaaaagaat ttgaaacttc tcttgtattc gatatcactc atgaattgag tttagttgct
    14101 aatgcgatgg aatcggtcat cgcccaaaaa gattatttag aagagttggc agaagtatat
    14161 ctcagcgata caagaaatgc cttttacatt ggacgtggtg ctgactacaa cgtttctctt
    14221 gaagctgctt tgaaattgaa agaaatcagt tatatccagg cagaaggttt tgccgctggc
    14281 gaattgaaac atggaacgat tgctttgatc gaagaaggaa caccagtgat cggaatcatc
    14341 agtgaagaag tgactggtgc gcatactcga ggaaacctga aagaagtcga aagccgtggc
    14401 gctaaaacac tagttattgt ctcagaggga ttagaaaagg aaagtgacca gctggtgcta
    14461 ccaactgtcc acccttatct aactactcta accacagtcg taccgactca attacttgca
    14521 tattacgcaa cgctattacg gggattcgac gttgacaaac cacgtaattt agcgaaatca
    14581 gtgacagtag aataataaaa gaacaaggac cagtgtcaga catctgacac tggtccttgt
    14641 tcttttattt tttatgaaaa tatctcttaa tccagtccga tttcttcttt gactactgcc
    14701 gcgattttat cgacatagta attaactttc tcatcagtag gcgcttctgc catcactcga
    14761 agtaatggtt ctgtaccgga aggacgaact aggattcttc cttctgatcc catttcagct
    14821 tctgcctctt caatcacttt tttgattgcc ggaacatcca tcgcaccgtt tttatcactt
    14881 acgcggatat tcactaattt ttgcggatag attgttacct ctgctgcaag ctctgataat
    14941 ttcttacctg tctgtttcat gatattcagt aactgaattc ctgaaagcat cccatctcca
    15001 gttgtgttaa aatctaagaa aatcatgtgc ccagactgct cgccgccaaa gttataatcg
    15061 tttttgcgca tttcttctac aacgtaacga tcaccaactt gtgtaacgac atctttcaaa
    15121 ccgattcctt ccaccgcttt gtggaatcct aagttactca tgacagtcgt aacgatcgta
    15181 tcttttttca atcgattttt ttctgccaag tatttagcac agataaacat gattttatca
    15241 ccgtcgacaa tgtttccaag ttcatccacc gcaataatac gatcgccatc gccatcgaaa
    15301 gctagtccag catctgctcc tttttcaacg accatctcag ctaaacgttc tggatgggta
    15361 gagcctacgc cgtcattgat atttaacccg tctggatttg tgcccattgt ataaaaatct
    15421 gtttcaagat cggcaaacaa acgattaaca cttgttgaag ttgctccgtt tgctgcatcg
    15481 acacaaatgg tcaaaccgga aaggtcaccg ttaatcgttt gtacaaggaa ttgagaatat
    15541 ttcaataagc cttctggaaa ttcttctaca gttcccaatc cttcagctga tggacgaggc
    15601 aattcatcca cttcagcatc taataatgct tcaatttcta gctcttggtc atctactagt
    15661 ttaaaaccat ctgcaccgaa aaatttgatt ccattatctt gtgctggatt atgagaagca
    15721 gaaatcatga cccctgcgct tgctttttgc aaacgtgtca gataagcaac acctggtgta
    15781 gagatcacac ctagctggaa cacttcgata cccaccgata aaagtcctga aatcagcgct
    15841 tcttctaaca tttgacctga gattcttgta tcccgcccta ctaacacgcg tgggcgtttt
    15901 ccttcttctt catgctggct taggacataa cctccgcacc gtcccagctt aaatgcaagt
    15961 tctggcgtaa gttccttgtt ggcttcaccg cgtacgccgt ctgttccaaa atatttaccc
    16021 atagttgtaa aattactcct ttaacttaat tttctgagga atttgttctg ctcatttgtg
    16081 ttgattcgct tgtttctgat gtttcttctg tacttgtggt cgagctgctt gtcccagatt
    16141 ccgaactgga agaaatggtt gttgtcgatg gttccgttcc tgaatcagca gatgaaatac
    16201 ttctactcgt acttgtattt tcttgtgtcc gactgctcgt cgaagcagag ctactgcttg
    16261 acgtttgcaa cacgggtgta atctccacac taactgattc gggcttgata cttaacccct
    16321 cttctactgg tatttcaatt ttacgagttg ttcgatgatt gatacctgag acattgatcg
    16381 gtacagcaat actgtcaccg atcgatgcaa gcaaatcgct agagccgctt acttctgctt
    16441 ccagttttga aaaactgaaa tcatagcttt ctaccccggc agctggtgtt ccttgctgta
    16501 tcccataaag tcgtaccgac ttgcttggct tgatcgaatc agctctcact gttacttgca
    16561 ctggatcaga gacgatactt aatacttccc ctgctgcatt cagcgcctgt attgggacct
    16621 cactattaat cccatcatct gtgaccatcg aagcgtccac atcagctact actcgtttga
    16681 tttctgccaa tgttttgtca cctgtagtga tagagacttt tttaggacta acggaagtat
    16741 catcaagttc atatccagat ggtgtgaacg ttgaaggaat caacgtttcc acatcgaaat
    16801 cttttattac ctttttctca accgttactg taatagtcga tggttcgatc tttgctgtga
    16861 cagcactgct caaattttga atttttagcg gaacttcatg tgttccttcg cccagcttag
    16921 tgatatctgc tgtgacgcgg aacatccgtg tatcttcgtt tgcctctgca ttcaactgaa
    16981 ctcggtttgc actggaaagt ttgacagtca ctgcattttc atatccatgg atatagtagc
    17041 gctttgaatc ataagaaatc ttgatcggca cccccgtaac tgtttgatta tacgcttcat
    17101 ttccagaaag cgtatttgga acggactggc catttgcatt gaagtacagc gcaatactga
    17161 acagcaatgc aataaaactg taaagaatgc ttgtccgttt ctcttttgag atcatttgcg
    17221 atcccctccc ttagtcatac cgtctaggaa agattgaatc acgtttttct tgttttttgt
    17281 tttctcttca gcaattagtt ctctacgcaa gattgccaaa tattcttgct gggataagcc
    17341 agccatcagt tcattatcta acgtgatgct gacgtccccg gtttcttcag agacaacgat
    17401 ggtaatcgcg tcactgactt cgcttatacc gacagctgcc cggtgtcgag tcccgaattc
    17461 tttagggatc aacatacttt ctgatagagg aagataggcg cttgctactg cgatctttcc
    17521 ttcttttaca ataactgcac cgtcatgcag cggtgtatta ggaataaaga tattgatcag
    17581 cagctcccca gtgatatctg catctaaagc aatccctgtc tcaatatact catctaatcc
    17641 agtatgacgt tcgatagtga tcaacgcacc gatttttcgc ttggacatat attggatggc
    17701 tttgtcaaaa gacaagatca tcttctcatc ttcctgctgt tcgctttttg attgtttgaa
    17761 aagcgaactg cgtccaagat gttccagtcc tctccggatt tccggctgaa aaatcacgat
    17821 agcagctatc accccgtacg taatgacttg gttcatcagc caagacaagg tgtgcagacc
    17881 aatgatttcc gctacgattc ggataacaat aaaaatagct acacctttca gaagctgaat
    17941 cgcttttgtg cctcgcagca gttggatcag tttgtataca agataccaga caaccagaat
    18001 atcaagtatg ttcaccaaaa aattcagaga gaacaagtca ccggaaaaca tttgctgcca
    18061 atagtccaaa ttaaaaagtt ccccgatttg aaatgacata aaaagcctct cttctctttt
    18121 gagatttcct ctctagtata tcatatgttt taattttaga ggctgatatg ctcgtatttt
    18181 tcttgagaaa aacgcataat tttcaagaaa attgcaagta tgcgttttat ttttctctgc
    18241 aataaatatt tttaaacttc agagaattat tcattatatt ttttctgtta tttttccttt
    18301 ttttcttttg tagcttgttc cattttttta cgtcgttctg ctctggcagc tcttctagca
    18361 gctcttcttt cttctgcttc aaggtccgtc gttttttctt ttctagtctt aacgggtaca
    18421 tcgatagatt cctctggttc aagtcgtttc ttgatttttt ctaattctcc cgtcagtgca
    18481 gcatagttgt aaaaacgtgt tttagcgtcg tggacagtct tttcaaataa agcttttgca
    18541 agatttggat ttgcgttatc tagcgaggaa aagcgaactt gtttcctcat gaaatcaggc
    18601 atagcagaga aatcaggttt tttgtagtcc aaagtcattg ggttcttgtt tttttcgacc
    18661 attgtagggt tatatcgata taatgaccaa taaccagaag aaacagcttc ttttgcttct
    18721 ttaagcgatt ctttcattcc gccaagcaag ccatgtgtga tacaaggcgt ataggcaata
    18781 atcagagaag gaccaggata cttttctgct tcttcaaatg ccttgatcgt ttgcatttga
    18841 ttggcacctg aagcaatttg cgccacataa acatttccat atgtcatcgc catcattcct
    18901 agatctttct ttgaagcata ctttccacta gccgaaaatt tagcgattgc cgaagctggt
    18961 gttgcttttg aggtttgacc gccagtattg gaatagactt cgttatccaa aacaaggata
    19021 ttcacgtctg ctccgctggc aagtacatga tcgatcccgc caaaaccaat gtcgtaagcc
    19081 catccgtctc caccaatcat ccattggctg ttttttacga atagatcctt tctcttgtat
    19141 atatcctcca gttcagggaa aattgatcgt tcgctttcga tcgcagcttg cagttttgct
    19201 gctctttgct gcgttccttc actctcaaac atgtgtacta accagtcttc catcaattct
    19261 cgtagttctt ctgatacttt tggcaatact tctttgattg aacttgccaa agcctcgcga
    19321 cgagtctgtg ccgctagaag cataccataa ccaaattcag cattatcttc caataaagaa
    19381 tttgaccatg caggcccttg accacattca ttggtcgtat aaggagaagc aggagaagca
    19441 ccgccccaaa tagaagaaca gcctgtcgca ttcgcgatca tcattcgatc accgaacatt
    19501 tgtgtcaata attttacata aggtgtttcg ccacagcctg agcaagcgcc cgagaattca
    19561 agaagcggtt gattgaattg tgtagacaag acagtattct ttcctttgac tggatttgct
    19621 ttttgtctta atgtcattgc aaaagcccaa ttcacagctt gttctttctg ttcttcataa
    19681 ggcttcatca ctaaagcttt ttcttttgcg ggacaagcct ctacacacag accacaacct
    19741 gtacagtctt ccactgaaac ttggatacgg tattttacgc cgtctgcgcc tcgcatttct
    19801 ctcacgataa atccttctgg cgcttcttcc atttcctctt catctgctaa aaatggacgg
    19861 atggcagcat gaggacaaac aaaggcacat tcattacaca tcgtacagcg atcactaatc
    19921 cactcaggta cttctaaagc gacgccccgc ttttccatag aagccgttcc taaaggcatt
    19981 tcgccagttg tcattccatt tgctacaagt gtaccaacag agagttgatt tccttcctgt
    20041 gcattcactg gttcaagtat ttcttgtata taaccagtgt tccttttatt gattttttca
    20101 ggcatctcca acgttctcca atggtctggg atcgcgactt gatgaagcag ttcggtcgtc
    20161 cgatcgattg cttgtatgtt tttttctacg atagccattg atttatgtcc atagcttttc
    20221 agggcttctt cttttagcag cggaagcacc tcatcaaaag gaatgatgtc agctaattta
    20281 aaaaaagccg tttccattgc cgtattgatc cggcgtccca aaccgacttc ttgggccaat
    20341 ctcatggcat tgatcgtata gaatctaatg ttattttcag ccaagtaacg ttttaatttt
    20401 aagggcagat gcgtttctaa ttgttcgtcg ctccaaagag tattcaataa aaaaatgcca
    20461 cctggcttca atcctttgac caagtcatag ctatgcaggt aagctggtgt gtgacaggca
    20521 acaaggtccg catgctcgac aagatacgct gaacgtatcg gcgtatctcc aaaacgaaga
    20581 tgagagacag tcagaccgcc agattttttc gaatcataat aaaaatagcc ttgcgcatat
    20641 ttatcggtgt gatctccaat aatcttgata gctgatttat ttgctccaac tgttccgtca
    20701 gatccaaatc cccaaaactt ggcctggaat gtttgcggtt ctgtcaaatc cagactttcc
    20761 tttggttcca aagattgata agtcacatca tccacgatcc ctatggtaaa ccgttttttg
    20821 cgtatgcttg ggtctttttt caattcatca aaaactgctg agatttggtc gggagtcaca
    20881 tctttagacc ccaatccgta gcgaccacca ataacagccg ggcgaagttc agaatcatat
    20941 aacgcacttt gaacgtctaa taagagaggt tctccacctg cccctggttc tttacttcga
    21001 tcaagaacag caattgtttt tactgtatga ggcaactttt gcacgaatgt ttccactgga
    21061 aaaggacgat aaagatggat attcaaaaaa cctgttttcc gtccgtttgc attcaagtaa
    21121 tcaatcgttt gttcaatcgt ctgtgcaact gatcccatag aaacaatgac ttcttccgca
    21181 tcgtctgctc cgtaataagt caccaagtca taagaagttc ctcgtaatcg gttgatctct
    21241 ttcatatatt tttgaacgat ttcaggtaat ttatcataat aggggttgat cgtttcccgt
    21301 tgttgaaaat ggatgtctgg attctggttc gtaccactaa cagtcggatg attgggattc
    21361 atccctcgtt tacgaaatga agctaatttc tcctgattga tcagaggagc taattcttca
    21421 tattccaaaa cttcgatttt ttgaatctcg tgactcgtac ggaatccatc aaagaaattc
    21481 aaaaaaggga cttctccttc aatagaagca agatgggcaa caggtgctaa atccatcact
    21541 tcttgtacac tgctttcagc taacatagca aatcctgttt gtcttgcagc catcacgtct
    21601 ccgtgatctc cgaaaatatt caacgcattt gtcgtcactg cccgactagc aacgtggaaa
    21661 acacttggca gtagttcccc agcaatcttg tacatattcg gtatcatcaa aagcagccct
    21721 tgtgaagcgg tatatgtagt tgtcagtgca ccagttttca atgatccatg gaccacacca
    21781 gctgctcctg cttcggattg catttctact acttttactg gttgaccgaa tacgttttct
    21841 tttcctgctg ctgaccattg atctactaat tcagccatgg tcgaactggg tgttattgga
    21901 tatattgcag caatctcggt aaatgcataa gaaatatatg cggctgccgt attgccatcc
    21961 attgttttca tttttcgcat gattcatcat ccttgttcat attctctctt attatctgtt
    22021 ccattataac gtaaaacatg ccattttttt cttttattta taaaaatatt ttacatgtat
    22081 ctatttgaat tggtcatcta atttttagac aaacagtcta aatcgttgcc tttttttgtc
    22141 agacatgtat aaactataag cgaaagaggt gttttaacat ggatatatca gtgattgatg
    22201 caacgaaaac aaatacacaa aaaggaatcc tttatggttc atcgaatgct cctaaaaaaa
    22261 tggtcgaatt catcaactta gcttgtccgt attgccgaca atggtttgaa gaatcttatg
    22321 agctattaga agaagcagtc caaagcgggc aattgcagcg agtgatcaaa ttgttcgata
    22381 aagaaaaaga aagcttacag cgagggaacg tgatgcaccg gtatctcaca atcagcgatg
    22441 gccaaaaagc gatcaaagag atcaaacaaa tattcgatac tcaagacgag tggaaacatc
    22501 tttctctcca agaagtagcg gattttgcag tagataaact aaagctgaca gaacaaaaag
    22561 acgaacagct gagccaagct gtcatcaacg aagctgagca agcacatatt cgatttgttc
    22621 caactgtcat cttgggtaaa gaaatctttg atgaatctat ttcaatagag gaattaaaag
    22681 aattgatcca aacaaaataa aaaaataagg agcgtg
//
LOCUS       DASNNU010000042        22645 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.042, whole genome shotgun sequence.
ACCESSION   DASNNU010000042 DASNNU010000000
VERSION     DASNNU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.042"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(293..1756)
                     /locus_tag="U8116_001512"
                     /pseudo
     CDS             complement(293..1756)
                     /locus_tag="U8116_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(1820..2863)
                     /locus_tag="U8116_001513"
     CDS             complement(1820..2863)
                     /locus_tag="U8116_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN8434516.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(3024..3707)
                     /locus_tag="U8116_001514"
     CDS             complement(3024..3707)
                     /locus_tag="U8116_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEN8434517.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            3902..4831
                     /locus_tag="U8116_001515"
     CDS             3902..4831
                     /locus_tag="U8116_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN8434518.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            4892..5683
                     /gene="phnC"
                     /locus_tag="U8116_001516"
     CDS             4892..5683
                     /gene="phnC"
                     /locus_tag="U8116_001516"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEN8434519.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            5655..6434
                     /gene="phnE"
                     /locus_tag="U8116_001517"
     CDS             5655..6434
                     /gene="phnE"
                     /locus_tag="U8116_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEN8434520.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            6431..7213
                     /gene="phnE"
                     /locus_tag="U8116_001518"
     CDS             6431..7213
                     /gene="phnE"
                     /locus_tag="U8116_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEN8434521.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            7216..8019
                     /locus_tag="U8116_001519"
     CDS             7216..8019
                     /locus_tag="U8116_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN8434522.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            8103..9650
                     /locus_tag="U8116_001520"
     CDS             8103..9650
                     /locus_tag="U8116_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEN8434523.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(9947..10144)
                     /locus_tag="U8116_001521"
     CDS             complement(9947..10144)
                     /locus_tag="U8116_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434524.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(10220..10420)
                     /locus_tag="U8116_001522"
     CDS             complement(10220..10420)
                     /locus_tag="U8116_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN8434525.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(10540..11727)
                     /locus_tag="U8116_001523"
                     /pseudo
     CDS             complement(10540..11727)
                     /locus_tag="U8116_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11772..12898)
                     /locus_tag="U8116_001524"
     CDS             complement(join(11772..12670,12670..12898))
                     /locus_tag="U8116_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN8434526.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(13302..14084)
                     /locus_tag="U8116_001525"
     CDS             complement(13302..14084)
                     /locus_tag="U8116_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434527.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(14149..14985)
                     /locus_tag="U8116_001526"
     CDS             complement(14149..14985)
                     /locus_tag="U8116_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN8434528.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(15003..16412)
                     /locus_tag="U8116_001527"
     CDS             complement(15003..16412)
                     /locus_tag="U8116_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN8434529.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(16426..18249)
                     /locus_tag="U8116_001528"
     CDS             complement(16426..18249)
                     /locus_tag="U8116_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN8434530.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(19092..19418)
                     /locus_tag="U8116_001529"
     CDS             complement(19092..19418)
                     /locus_tag="U8116_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434531.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(19464..19826)
                     /locus_tag="U8116_001530"
     CDS             complement(19464..19826)
                     /locus_tag="U8116_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEN8434532.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(19863..20077)
                     /locus_tag="U8116_001531"
                     /pseudo
     CDS             complement(19863..20077)
                     /locus_tag="U8116_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            20205..20540
                     /locus_tag="U8116_001532"
     CDS             20205..20540
                     /locus_tag="U8116_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8434533.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(20616..22613)
                     /locus_tag="U8116_001533"
     CDS             complement(20616..22613)
                     /locus_tag="U8116_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434534.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT         6776 a         4560 c         3983 g         7326 t
ORIGIN      
        1 cagaaacttt ctcatacact tggctttcct taagaatagc acataattta gttaaatttt
       61 agttcttttt tatatttcta cagcctgtta ataagtaaaa ttttactttt gtataatgat
      121 ttaccccaca gatccttcca tatagatttt tgaattacac actagtttaa actaaaacaa
      181 acaaggaaaa acttaccatt taatcataaa attagctcat atgaatatat tagaatgtaa
      241 aatagactgg tcaaaatctg gtcaaaaaag aggactaata caggtagtcc tctcagactg
      301 tagacaaaag ctgttccaaa ctcgtaactc gagcttggaa cagcttttta tctacttcgt
      361 ttcgaatttt tgaacaaaaa aataaaggct ccttgccttt ccatagccag gtggctaatt
      421 tctttaaatt cattgcagca aacactagca tcgtatgcgt cgcgactttt tccaagcctc
      481 gatacttggt ccaacgcatc ccatgctttt cttttgcatc cgcaaatatt cgttcaatcg
      541 tttgtttccg ttttttgtaa attgatttat tcagatctgt taggcgaaga tgttccactt
      601 catccatcag atctccccaa acatgtctcg tgatgactcg ttgatgattt ttacttttgg
      661 tacattggtt tagcagagga cagttcacac attcttttgg attggatttg tattctcggt
      721 acctttctcg agtaaccgtt gaaaaagcga gcatttgatc ttgcggacaa atataacaat
      781 caaaatactc gtcatagaga aactcatttt ttgataatag cccctttttc cctttgggcc
      841 ttgaataagg caaacaaggt cgaaggttta aatgagtcaa aaagtgaaca aacttaggtg
      901 tcttatactc agcatcagcg acaacacaat tgatttcagg aaaaagtctc ttcgattgtt
      961 tgatgagctg aaaggcgact tgactgtcat gaatatttct aggagtgatg attgtgctaa
     1021 gaataaatcc attgtcgtcg cattaagtat ggacagaata agcaaattgt ttttctcgtt
     1081 cgcctttgac gtagtagccg ctttccggat cagctttact tatttttctt tctttcagct
     1141 tcacttcctt ttctaagtcc aaagtaaaag ggcgttttcc agcctctatt cgttgacgat
     1201 tgatctcatc ttctaattca tcctgatagg ctttcgcttc atcatgggtc atttctttcg
     1261 taaacttatg tttattggcg ttagctttga tgtgggtaga atccagatac aaattatcct
     1321 ctgttacaaa acctgcttta actgcttgtt ctaaaatata ggcaaaaata tcctcaaaca
     1381 ccgtcgtttc tctaaatcgg cgaacataat tctttccaaa agttgagaaa tgggggattt
     1441 tctcctcgaa ggagtagcct aaaaaccagc ggtaagcgac attggtatcg acttccttaa
     1501 tggtttgtcg catggaacgg ataccgaata aatactgaat aaacaccaat ttgatcagaa
     1561 tcactggatc gatacttggg cgacctaaat cggagtaagt ggcttcgacg attggataaa
     1621 taaattcaaa atcaagcgcg gcatcaatct ttcgaacgag atgatcttga ggaactagat
     1681 catcaatcgt aagcatcgca aattgatttc taccattcgt attgttttta gacatcatag
     1741 ctatcactcc ttgcattcgt tttcataaat ctattataaa agaaaatact gtagacatca
     1801 tccataaaat ggactttgtc tacagtctga aatcaccact gatgattttc tcttttgcgt
     1861 ctgaaactgc ctgataggct tcctctgata attgtccttt tgtcagatct accccttctt
     1921 ctttcaaacc aaaggtcaaa tgcttgccgc caggaaattt atcgttatga gaagtttctg
     1981 caaattcttt gacttctgca ccgacatttt tctttgtaga agtcaacgtg aaattccctg
     2041 tctcgccgtt ctccatttta taatcccctt catccgcttg atcgcgatct acaccaatca
     2101 cccatacttt tctgctatag tcgttttcat tgcgggcttt tgcttcttgg aacaccccag
     2161 ctccagtact tcctgccgcc tgataaagaa tatcgactcc ctctttgtac atagaagcag
     2221 caatagcttt tcctttatct agcgaatcat aggatgcagc atattgtacg ttaacagtaa
     2281 tttccttgtt cagtgctgtg gcccctgcct taacgccagc ttcaaatccg ctttggaaac
     2341 gttggatcac gggcccttcc attccaccaa taaagcctac ttgatcactt tttgtcgtat
     2401 atgcggcagc tagtcctgct aaatacgcac tttcttgatc tttaaaggtt gctgatgaga
     2461 tattcttttt cccagtaatc tcaccatcta ccaaggcaaa tttcaacttt ggattatctt
     2521 tagcagcatt ttccagttcc tctgcttgca agtagctcaa gctaataatg gtggtaaagc
     2581 cgtttctgat tgcctgatcg atattggtac tgttatcctc tggattgttc gtttctatat
     2641 acgtaaaatc tttttttgct gtcagctttt tctcttttcc ccaagcctct aatccttccc
     2701 agccagcttg attgaaggag cggtcatcaa ccccgccaaa tccaagaacc aaagcgattt
     2761 tacttttctc tttttcactt tgcttctccc cctgagactg actacagccg actaacgtta
     2821 ctaatgacac caaaaacaac aatgaaacaa gtattttctt caaaattttt ctcccctttt
     2881 tatgtgtctt gacacattga acttttaaaa gtatagcata ccaatatata gaacactact
     2941 cttcaacgaa gattttccgt ttcccaattt ctccactatc aaaaaaccag caatcacttc
     3001 tgactgctgt ttttttatag ctgttattct gcttttactg gactacttat attctcagct
     3061 aagtcctcta aagcttcgat atcatgaatc aagtaataat tctttttctt ttcgataata
     3121 ttctcctcac gaaaattcgt gattgttcgt aaaagatgtc gataactgac atttaagatt
     3181 gaggcgcagg tggttaaccg aaatgagaaa cgttcgtttt cgtgatgggt caaaataaac
     3241 tttgctaaac gagtttcaac aggttctgtc agtgagttcg ccaaatttat cccggaattt
     3301 aaacgataac ttagtaactg gcaaatattc tgcaaaaagc gaacatcttt ttgaagctgt
     3361 gttcgatatt gatttagcgg aatgctgata cagatgcact tagtcactgc tttaacgctg
     3421 cttttcggcg ataattccca caatgaagag gcttcgccga gtggtgtagc gggctttgcg
     3481 taatcgatgc aaatattttg tgtatcagaa gaatacgagt acaccattac cgtcccatcc
     3541 actaaaaaat acaaataatc tgaggttttt cccgtttgaa tgaggtgctc ttccttttca
     3601 aaaaaaagta aagaagccat tgccaatata tctgaatcca tataatcctg caggttgtgc
     3661 ttctcaacgt aagcagttaa ttgcttcgta tcatggattt ttttcatgaa atcgtcacct
     3721 ctaagaagtt tttctttatt atagcttaga ttgtttttcg gctcaatacg aataattatt
     3781 tataattgtt tgtaaattgt atgtaaaagt tgtgtaaaat aggacgtgcg tcacttcatt
     3841 gattttgatt ccctgctatg atttagacgt attagaaatt atatccaaca ggaggagaaa
     3901 aatgcagttc aaacagttag caaaagggtt catcggagtc atgttattga ccactttggc
     3961 ggcctgcggt tctgggaata caagtagcag ctcagaaaaa gagagcaaag aaaaattagt
     4021 tgttcaattc gttccaacaa acaatgatgg tagtatggag gcaaaagcaa aaccatttgc
     4081 taagtattta tcgaaaaagt tagatcgtga ggtagaagtg acgttagcta ctgattattc
     4141 aacgatcgtc gaagcgatgt cttctggtca agtcgatgtc ggaattatgc cgcctgcagc
     4201 ctatgttcaa gcaaaggatg ctggagctgc agatgcaatc ctgacttctc agctcggcga
     4261 ttatgatcaa gaaactggct tgccattaaa ggacaaactt acgaacacct ttaaaggaga
     4321 agtattggtt cgtgccgata gcggattgaa taagttaact gatttaaaag gaaaaaatat
     4381 cgcaacctta agtcctaact ctgctagcgg ctacatctat cctgtcgctg aattgaaaga
     4441 tgctgggatt gatcctactg cagatacaaa gatgaccact gtgaatgata ttccgagtga
     4501 gattaccgca gtgttgaaca agcaaatgga tgcggcgttt gtttttgaag gcgcgcgaaa
     4561 tgtttttgct tcgaatttcc cagataatga tttatttaag gatttaaaag tcctgtacct
     4621 gacaaaaggc gatattccga atgacgcaat cgctgtacag cctgacatgt caaaagattt
     4681 aaaagcgaaa attaaagaga ccttcttaaa tatgaaggat gataaagatg gcaaagaagc
     4741 aatggctttg tgggggcatc agggctatga agaagctgct gattctgcct atgacacgat
     4801 cagagactac acgaaaaaag cagcagaata aaacatcagt aagtgtcagc aagctttttt
     4861 ttgcttacac ttaaaaaaat ggagaaggat aatgattatt tttgacaatg tttcgaaggt
     4921 ctatcccaat ggcgttgttg gcttgaagga tattgactta acaattgaag atggagaatt
     4981 tgtttccatc atagggttga gtggtgctgg aaagagcact ctgctgcgca gtatcaatcg
     5041 cttggcagaa accaccaaag gagatattcg cattaatgga acctctatta caaaagcgaa
     5101 taaaaaagag ctgcgacaga ttcgccgcca gattggtcta atctcgcaaa gcttcaatct
     5161 ggtaaagcgt agtacggttc agaaaaatgt gctatctgga cgtttgggct attactccac
     5221 ttggaaaagt attttaggtc tttttacgaa ggaagactac cagcagacaa aggaagcact
     5281 ggaaaaagtt ggtttgtctg ataaattgca gacacgcagt gatgagctca gtggtgggca
     5341 acaacagcga gtatccattg cgcgagcact cgtgcagcaa gcccccatta ttttagcgga
     5401 tgaacccgtt gcatcattag atccaatcac gactcaaaag gttatgatgg acctaaaaaa
     5461 tatcaatcaa acgatggata aaacagtcat tgtcaatctg cattcagtcc ctctggctcg
     5521 tgagttttct acgcgtgtgc tcgcgttaaa agctgctcag gttgtctttg atggtcgtcc
     5581 agacgactta accgatgaac gcttagagca aatttatggc aatgcaatct ttgaagaaaa
     5641 ggcaggcgaa atcgatgagc ttgaaagaaa ccgttcactt taaatggtat aaacacctgc
     5701 ttattcttct gttcttaatt gtttgttttc aaggaagtgc ctggatcacg tcagctgatt
     5761 tttctcaggt ttttcaaaac tccaatcaaa tgactgtctt tttatcacgc ttcattcatc
     5821 cagactttag ctatttacct aaattagtcg agcctatggt gaaaacactt caaatgtctt
     5881 tgcttggaac ggtcattggc ttaattttag cgatccctgt tagtttttta gcgaccactg
     5941 tagtgacaga aaaccgtctt ctttctacca tctttcgttt tcttttaggt ttagtacgga
     6001 ccatccctac cctgctttta gccgcgcttt ttgttgctgt gtttggaatt ggtgaagcga
     6061 ctggcgtgtt gactatcgct gtctttactt ttggtatggt gtcacaatta atgtttcaag
     6121 caattgaaac cattgacttt ggcccgattg aagccgctgc ggctgttgga gcaaataaaa
     6181 tgcaaatcgc tatttggtcg atcgctccac aagttttgag tcagtttgcg agctatgctt
     6241 tttacgcgtt tgaggtgaat gttcgtgctt ctactgtcct tggatatgtc ggagctggtg
     6301 gtattggcgt tattctaaat tcctcacttg cgcttttgaa ttatgaacga gtttcaatca
     6361 ttattctaac gatcctagta actgtcgcga tcgttgataa attaagtgaa cgtgtcagga
     6421 ggtccctgtt atgatgcttc gtgaaatgaa cagaagaacc cttttatcat tagttgcttt
     6481 agtgattctc gttattttgt cggccgctac tctggattac tctggattaa gcaccttctc
     6541 tccttccatg ggaatggacg ttttgagagg gttaggtcag cctgattggc agtttctata
     6601 tgatggcagc ggcgaggatt tgattagttt gctattatta accattggaa tcgcctgttt
     6661 agggacaatg atcgctacgg tattagcgat tcccattacc ttgatcagtg cggttaacct
     6721 ctggcaagca catccatggg taacaaaaat tggcaagctt atctgtaatg ttttacgagc
     6781 ttttcctgaa ctagtttttg caattatttt tgtgaaagtc gtcggacctg gcccctttgc
     6841 tggtgttatg gcgattggcg tccatcagat cggaatgctt ggcaaacttt ttacagaaga
     6901 aatggaagcg atggatgagc gacttgttga agaaatgcat gcggtaggag caaatttttg
     6961 gcaaacgatt ttctttgttc gcatccccta tttgatgccg atttattgtt ctttagcatt
     7021 aaatcatttt gaaatagcgg ttcgtagtgc ggctacctta ggcttagttg gcgctggcgg
     7081 aatcggtgct ccattgatat tcgcgattca aactcggtct tggagcaaag tgagtattat
     7141 tttgattggc gtagtcgtaa ccgtgtttat tttagatcaa ataaccggaa ttataagaaa
     7201 gagattgaga taagaatgaa tatattgcat atatcagata tacactttcg cagagagtat
     7261 gaggcttgtg aagaaggcta taagggaatg ctggctacga tgcagaatcc ccttatccct
     7321 ctggagcaat gcattcatca tctcctgcag caaacaccct tggaccttgt gattatcagc
     7381 ggagatttaa ccgaagatgg cgaaatagac gattatcgat acttgaagac ttggctggaa
     7441 aatgcgctag gagaaacgac gattcttgtt actctaggca atcacgatat caaagaacat
     7501 ttttgggtag gctggtgcga tgaagccgcc tcttctgccc cgtacaacca gatagtcaaa
     7561 tatcctgagt ttacagtgat ttcctttgat aattcaagct atggatacgc agatggtata
     7621 gtggacgatc agcaatttca atggctgaaa aaagccttag agcaagaaaa agaccaaccg
     7681 attttcttgg tcacccatca tcatctactg tctcaccaaa gcagtatacc aaaatggcct
     7741 ggtaccgaac gtttcttgaa gctaatcgca ccttacgata ttcgctgtat cctaaacggt
     7801 catacccatc acacatttac tgatgaaatt aatgggatcc cctattttac tgtatcgagt
     7861 atgtcctttg ttggtgaaga tgaaggcgat ggcttcgtac gctttgaaga acgtcatggg
     7921 tataaccttt atcatctcga tcaaggaagg attgttcgcc agacaacaga aaactttatt
     7981 ccaggcaaca ttttaaaaac acttcaaatg aaggattaag gcattataaa tcaaaaaaag
     8041 agaccgaaca gctgaatacg cttcggtttt tctattttga gtcagctaaa ggagcacgca
     8101 gaatgaacat tacgttttta gagacaagtg atatgcatgg ctatgtttca ccaacagact
     8161 attccggcag aaccgatctg gccttaggaa cggctaaagt atcgtcgaaa ctgaaggaat
     8221 tacgggcaaa agcagatgga ccggtaatta cgatcgaaaa tggtgatttc atccaagggt
     8281 ctcctttgag ttattatctt gctaaagaat atggttcagc caaagagctt acagacttga
     8341 ttaaccaaat gagctatgat gttcaagtgt tgggaaatca tgagtttaac tatggtgtgg
     8401 attatctcga ggaagcgatt aagaattatc aagcgcctat attaggagcc aatatggtga
     8461 acaaaaatga ccaaccatac tttggaaacg cctataagat tatcgaaaag gtgggtgtaa
     8521 aaattgctat cttgggatta acgacacaat acattcctca ctgggaaaag ccagaaacct
     8581 tagcaggaat tacgtttaag agtatcgtcg aaacagcaaa agaatatgtt cctctattaa
     8641 ggaaacaagc agacttggta atcgttagtt accacggcgg atttgaaaaa gacttgttaa
     8701 ccggtgagga cacagaagcg ctaactggag agaatgaagg ctacgagcta ttgcagcagg
     8761 ttgaaggcat tgacgccctt ttcaccggtc atcagcaccg gaaaattgct gaaacggttc
     8821 atggagttcc cgttgttcaa ccaggctatc gtggcgagtt catcggtgaa attcgtctta
     8881 ccgtcgagaa aaaagagggg caagtggttg ttacagatca ccaggttgct ttacactcag
     8941 ttgaaaaatc agagcctgat ccagaaattc ttgcagcgat cgtaccggta gaagaaaaac
     9001 tagagaaatg gttggatcaa cccttaggta aagtgaacgg aaatatgcgt attaatgatc
     9061 cgatgcaggc tcgtcttgag gaacatccct atgtagagtt tattaatcgt gtacaattat
     9121 ttgccagcgg ctcagagatt tctggaactg cgttattcaa taatgaagga aaaggctttg
     9181 gtgagacgat cacgatgcgg gacgtcatca ccaactatat ttatccgaat actctggcag
     9241 tgattcaaat aaatggtgca gatttgagag cggcgttgga gcagaccgca aattatttag
     9301 ctgtagaaaa taaccaaatc gtattcaatc cagcctttat tcagcctaag ccccagtact
     9361 acaattatga catgtatgaa gggattcagt acactattga tatgaaacag cctgttggac
     9421 aacggattac cgaacttcgt tttaaaggtc accccgttct tccgcaacaa atgctggaag
     9481 tggtgacaaa tcaatatcgg gcaatcggcg gaggaaatta tgcgatgttt ggtgcggaga
     9541 aaattgtccg agaaatccaa atcgacatga ccgaattaat tgccgactat ttgaatattc
     9601 acccacgcat cgaagcaaaa gtgaatcata attttcaagt gattttgtag tatataaata
     9661 aaaaattcaa aaaggtgtga gcagtttttt gcccacacct ttgttatgcc aaattgttag
     9721 gctgtctgat ttccatccag ccattcttta gccagcgatc tacctcatca aaacaaaaaa
     9781 ttttcctctg atggttcctt aaataagact attttgatcc tttttccatg aataagattt
     9841 tgcatcagac aatacttcat ttctgaaaaa gttgcggcac caatatagcc taatacccct
     9901 ttccgatctt gattaaatac aaaaaataaa tccgctgctg cgatatttaa aaaaaagtcg
     9961 tcaagatttc tggatactct ttgtctagga tcggacatga gcgaggataa tcaattaatt
    10021 caatgccttt tttactgaaa tctttttcca tatctcgata ttcttcttgg aaaacaatac
    10081 ttccagaaat aacagctttc acaaaattgg cctccatttt tttatttttc agacactcca
    10141 ccataccgga tacctctcaa agaaaagaca tagcaaaaat caatagcagc tatctctcca
    10201 cggtttttta ctggtaattt tatttttctt ttggaaattg tatcaggaag gtcgttcctt
    10261 cattttttcg gctggtcact tcaattgaac cgtggtgcag gcgcactagt tccttcacga
    10321 ttgataatcc aagacctgac tcgcctatct ttgtattttt acttgacgga tcggctttat
    10381 aatagcgatc ccaaatgttt ttttgttcct cttcaatcat gccgatgcca ttgtcctcga
    10441 cagtaataaa tacaggctct ttgtcaaatg atgttggtaa agagaaatct tagaataatt
    10501 tggacagaac agaaagaaga aactctcttt ctgttctgtt tatttggtta aaaatgtttt
    10561 gccatgacga atcatgatcc gtagtttgaa attgtaaaag ctgcgaaagc cgtacgcatt
    10621 gcgttttaat actttgatgt ggttgtttaa acattccaat ggtccatttg aatagctgta
    10681 gtgaagcgca ttttggatct gtggcagaaa ctttttaaac gttcgtaaag tggttgtata
    10741 ggtttcagga agaccgctga cttctctgtt taaacagtcc tcaaataatg actgatctct
    10801 tttctttacc gcataaagaa tgtcttggta taactgatag ccttggcgca ggttgggaca
    10861 agcagataac agtcgatcga caatctcact ttcagtcaaa taatctttaa aactggcacg
    10921 ccaaaccctt ttttcgtagt ttaactttgc ttcttctttt tgtaggagtt tccaatagcg
    10981 tttcaatttt ttccctaacc ctcgttgaga gtgggaagtt cctttaagcc gtgccgtggt
    11041 ttctttcact cgatgattca agaaggttct gccaatatgt tggacgatgt ggaaacggtc
    11101 gatgatcaac tgagcattcg ggaaaagctt tttcaccaat gaaacataag gcgcatacat
    11161 atcaataaca atccattgaa cggcttcacg agtcgctaat gaaaaccgag aaaaatagca
    11221 ttctaaataa ggcaagcgtc gattttcgac aatatccaat aatgcatggc tttgtccgtc
    11281 catcatgata aaactcattg cactggcaac acttcgaacc gatttgaatt catcaaagca
    11341 taagaccttt ggcaaagcct gtttgaaagg attcaatggg cggtagaatc gcttcatgac
    11401 acgatagacc gaagaagtgg aaatattttt ctgacgagcg atttccgtca tggaagtatt
    11461 ctcttttaag cgagcaatga cagaccaacg aactttttga ctaatgaaac agtgtttttc
    11521 agcaacagag gtatccgcta cgaatgttct ttggcaagac ttacatttaa atcgttgttt
    11581 cttcaactcc agataagtgc gatactcaga cgcatcattc agtaacaacc gaacggttgt
    11641 cgtcccccaa cgaatgatcc tagaggggac gacacaaaga caatgggaac actcttctgg
    11701 cttgtacaga agagttcctt tgtaaatcat ggatttgagt caatatcttg gacacatttt
    11761 agtagagacg gttaagccat tttttctttt gctaattctt caaattcctg tggggttaga
    11821 tagtgaatcg agctatggat tcgatttcgg ttataaaatc cttcaatgta gctaaatagc
    11881 gctcgatttg cttcttcaaa atctgagtag ctagtcgtgt agacttcttc ttttttcaat
    11941 gaggcatgga aagattcgat tccagcatta tcataaggtg ttccttttct actataagaa
    12001 tgccttattt tgttggtttt aagccattgt tccacttctc ttgctgtgta ttggctgccc
    12061 aagtcagtgt gtagaatcat tccttctgga atgtgataat gtatttttgc tttattcaat
    12121 gtttgaatga cacaatccac agtcattcgt tttgaaaatg tataactaat aatttttttc
    12181 gtgtgtaaat ccatgattga agataagtaa caccaaccat ttttcttagt aggaatgtac
    12241 gtaatgtcag ctgcccattt ctcacagatc gtttcagtag aaaagtcctg atttaagaga
    12301 ttctcttttg aaatgatcgg tttattagac ctttgaggtc tatatttttt aactacaatc
    12361 gaccttaaat ttaattgttt cattagcttt tggacatgtt taagagatac tgaaatccct
    12421 tcttttaata aaacttgatg gatctttgtg gcaccatagc gttgtttatt cgtaaaaaaa
    12481 attgttgaaa tcttcttttt caacttatta tttctgattt ctgtagctgt caacggttta
    12541 ttcttataaa aataatagac acttctaggg atttctaata accgacataa caaagaaaca
    12601 gtgtaatcct tacaccattt ttcaataaat tgaacaagcg aatttaggtt ttccgagaga
    12661 atatggctgc ggcttttttt aagatttcaa gttcctcatt taaacgagca ttttcttttc
    12721 tcagttcagc tacttctttt ccagtcagtc ctgtggactg atcaggtaaa tataaattct
    12781 tccatttgta aatcgttgct tcagataagc catattcttt cgctaaccct cgaacagaac
    12841 gtccagtttg attcagttca acaatcattt gtttaaaatt ttcttcatat cgggtcataa
    12901 aaattcatcc tctcgtagat aagtttaatt tattcttgtc tctacgaaaa gctgtctaag
    12961 atttgatact aacaccaagt tatctttaga accatttcag atacatatga aacaattaca
    13021 tatgattttt tcataaaaaa acagtactgt ctagtagtat aaaatataca aatttcttaa
    13081 aaattttttc actaaaatca ctacctgaaa cagtctaaaa cacttatata tcaacgttta
    13141 atgaaactat ctccgtttca tttcagatac aaataaatat agtcgggctc caaatccgaa
    13201 aaaaaatacc cccagtagct tatcctaagc tactggagat gtataagtac ggtcatataa
    13261 caacggggta gggtaaataa atgaaaaaga tggaagaatc attattcaat aactttttta
    13321 aatacttcgt tgtaatcagt tacctgtccg ttcttaaata ccacagaata atcttttttc
    13381 ccattttcaa gtaaccgaag tggaatgtct ttaccaactt gctctgtcac tgcattactg
    13441 atacctgtta gattagctaa taaatctgat aaagattgag catctgtaga aatctctgta
    13501 tcagtattta tcgtgattcc tttatcttca tttacaagct tccaagtagg tggtatagct
    13561 gtgtatgttt cgatgaatga atcaattctt tcatctatcg tcttcccagt atctacttct
    13621 gtctcattac tcttagatga atcgattctt tcatccatcg tctgctccgt aactgcttct
    13681 gtctcattat tctcaccgga aactctcttc acacccaaac ttatatattg tccatcttta
    13741 gtgtgcatag ttaaaattcc cacgctttta ctaataaatt cactataacc agttctttca
    13801 atagagactt caatgtattt cacactatca tactcaaaag aaaaatagac atgtaaaagc
    13861 attttgttgt ttacatctac ataataatcc tttgactcta cttgagacct tcctatacgg
    13921 aatacagctt tactgtctga tatctctata ccagcatcct tattattaat ctgccaatct
    13981 ccaagtagct cttctggcaa gctagtagtt tcttgtgaac actttaccac ttcactggta
    14041 gaggtcgaaa cattcacctc cgcactgtta gaagaatttt ccaaggaatc aggagcctta
    14101 gcacaaccaa caagtcttta tatattgtgc agccgatatt ttcttcattc agtacaccct
    14161 tgaaatatgc agtgttagat aaagcagttc gtcctgattt gctgaggcac ccatctcttc
    14221 ttgaacgtac tccttgattt tcaaggccgc ccgatactct ttcggaaaac gctgcttcac
    14281 tgaagccagc aaaaaatcat ccttctccgg caactcttcc ttcttaatca ttcgggcaat
    14341 aaaaaactgt aaatgcgtca caaatcttaa gtaaagcatc gaatctttat caaccttttt
    14401 ttcgggaaac agatatccta gcatagccaa gatatccttt agtgctctta ccgagagcat
    14461 ggtttcttcc atatttgctt gttcactttg ggcatttaca atgtgaaacg caatgtttcc
    14521 agcctcctct tctggcaact gaacattcat ttttttgttg atcaattgga tagcatagca
    14581 gccgatctca aattcttttg ggtaatactt ttgaatttca atcagcaggc gattttgaaa
    14641 aacaatgcct ttttgactgc gttcaatggc atgcttcaag tgatcaacca aggtaaaaaa
    14701 tagactttta ttcagcttaa agtcaaactt ggcgttggca taatcgataa ttcgcttgac
    14761 gcaaataatt agatcatccg gagcagcctc aaacagttta atgtaatcct tttttagagc
    14821 atcagaaagt gaatctaatt taaatacttt ttggattttg tcagccggaa ccaaatcgcc
    14881 tttctttagt tgaaatccaa tccctttccc catcaaaatt acttctttgc cttgcttatc
    14941 taagcaaaga attaaactgt tgtttaacgt tttaattact ctcattagat tcaccgttct
    15001 ctctaagcta agccatttct tgcgattact ttttgatacc aaaagaagct ttcttttttt
    15061 attcgtttta attctttaag attctcatcg gttcggtcaa catatataaa accataacgc
    15121 ttattgaagc cttgatgggt actaacaaca tcaatcgctg accaaggtaa ataaccaaaa
    15181 acatctacac catctgttaa agctgcttga atagccagaa tatgtttggc tagataatca
    15241 attcgatcct ggtcattaat tcgtctatct tcctcaacta tgtcgggtgc cccatagcca
    15301 ttttcagtaa ttaaaatagg taaatcatag cgatcgtaca atttcctcaa tgttaatcgt
    15361 agtccagtag ggtcaactac ccagccatat ggcgttttat ctacataagg attttcagct
    15421 gcccggtaga caccttcttc tcccaacatg atctgttgat cccccgctct agcggaaaca
    15481 tctgatccgt catttttgct ggcagcgatg gtggcagttg aataataatt aatcgcaata
    15541 aaatcgggtt taccggagga taaaattgcc tgatcctctg cctcaatttt aggatataat
    15601 ccacgatcat tcaaatagtt ttcaaacaaa taattgtatt ttccccgaac tgctacatct
    15661 aagaaagacc agcctctcaa tgtttcccaa ttatgggctg caattgcatc ttctggactg
    15721 gaggtagctt gatacatagc cgttaaattg attgctggac cgatttttcc tttaagattc
    15781 aactgatgat acaagtgaat tactctagct tgagctaata aaacatggtg attaatttga
    15841 aagagttctt tttttgaagg cagctctcca cttggtggca aaccaatcgc tccaggatga
    15901 aggatcatcg tgttttgctc attaattgtc agccaatagt tcaccttgtc accatactca
    15961 gtaagcaata cctgtacata gtccacaaaa taatcgatag ttgctcgatt taaccaaccg
    16021 ccctgctcct ctagaaaata aggtaaatcg aaatggtaca tggtgacaat cggttcgatt
    16081 ccctgattca ggcattcatc aattacttga tgataaaaag aaattccctt ttgattggtt
    16141 ttattatttt caatatccat aattcgcgtc caagcgatgg aaaagcgaaa agcttttaag
    16201 cctaattctt taaacagctg tatatcttct ttatagtgat ggtaaaaatc cgaggcaact
    16261 tcaaaattag ctaccccttc tgtatgctga tacttgtcaa taatagacgg ttgtttccca
    16321 tcctcctgat aggcaccctc tgcctggtag gcactaatgg aggcacccca taaaaacttc
    16381 tctggaaatg ctgaaagctg tttgtattgc acaaaaatcc tctccttaac taatttgcat
    16441 caatggttcg tgttccttta agcttccgct tgcttcacta attcctgcgt aattttcact
    16501 gttggtcact accacaatca ctgtatcatc gtagccagcg tttctaatgg cagcgaaatc
    16561 ggcgttgatt aataattgtc ctgctgacac ctgatctcct gcttttacaa atacttcaaa
    16621 gggttcccct tttaaattca cggtgtcgat ccccacatgg atcaataatt caataccttc
    16681 tttagaacgt agaccaatcg catgtttaga ttctaaaata gtaatgactt ctccctcaac
    16741 aggtgaataa atgctgtttt cttctggatg aatggctacg ccctttccca atagtccctt
    16801 agaaaagaca gcatcgttca cttcagaaat aggacacacg gttcctttta ccggattttc
    16861 aacaatgact gcttcggcta ttgccgtttg ctctgattca tcagaagtat cgtcaaatcc
    16921 aattagataa gcaatgataa aggttgcaac aaaggcgatt acacacgtaa taattgcgtg
    16981 aacaatattc attggatttt ctccaataaa ggctggtaag gctgctaagc caggggaaac
    17041 aaaggcatat ctgactaaac cgctcaagcc agcatacaaa ccagcaattg caccaccaat
    17101 catggaagca actaatggtt ttttcagctt caaggttact ccatagagtg aaggctcagt
    17161 aattcccatt aaagcagtga tcccagtgga cacagctagc tgcttcaact ctttgttttt
    17221 agttttcgcg gctacagcaa aggtagcacc tgattgagca atattagaag ccagcattcc
    17281 tggcccattg atcatttcag aacccgtact gctcaattgc atagtagcga ttggcgtcat
    17341 tgcccaagct gtacccgtaa ccaccaaaaa tggctgcaaa ccacccatca aaaatggaat
    17401 taaccaagct gctttgccgt taatccattc agcccccatt gctataagat cgttcagcaa
    17461 cgttccaaac ggtccaatga caaccaacgc taaaggcccc ataattaaaa ataccagcat
    17521 cggcttagta aagaatttaa taaacgaagg cgaaattcga tccgcaaagg gctctacata
    17581 ggacatgatc caaacagtaa agataatcgg taaaactgat cccgcataat ctgcagctaa
    17641 taccggaatt cctagaaaag aaacggcatc gccagtagaa aaaagctgtg aaatatttgg
    17701 atgaagcaaa atgccagcaa cagtaatcgc caaaattggg ttgacatcaa actttatggc
    17761 tgccccatag gctaatagta ctggcataaa ataaaaggct gcatcagcaa tagtattcaa
    17821 aatgatgtaa gtttgactac tgtctgatag tacaccagtc aaagtcaaga cggccaaaat
    17881 ggcttttacc atcccggcac ctgttatagc tggaatcatc ggtgtaaagg tggtagaaat
    17941 gacactaatc aatcttgaaa taatactttc ttgattgtct gaagaggggc cctttttaat
    18001 tgaagttccc acttcctttt gaatttcctt gaaggctgtt tggacttcat tgccaatcac
    18061 cacttggtat tgtccaccgc tatccaccac actaattaca ccaagtgttt tttctaaggc
    18121 tttcgtatcg gctttccttt tatctttgag ttcaaaacgc agtctggtgg cacaatgggt
    18181 taagctgacc acattttctt ttcccccaac ctgttgcaat acatttcttc ctacttctct
    18241 gtaatccatt attctctctc cttaaagcag taaaaaagac caagtcaaac cacgccccaa
    18301 tcaacattga agtgttagtt tgtcttggtc ttgcctgcat aatcagtaac atgccaataa
    18361 cgttattttg ctgtaatcca ataatagcac attatgatag cgcaatcaac agggaattta
    18421 attttttatc ttttcgtttg accgtatagg gtaatataac agttagatca catcaactag
    18481 ggtatatctg cgtatactta ctgctaaaat aagtatatct aatattaact ataccagtaa
    18541 aaccattcct acataaccag cgggaaattc ccaaaatcgt gaaagcatca aaaatgcata
    18601 taacggatca cacaacttca acaattctgc tcttgggaaa aattgatgaa tttggtgagt
    18661 aaaggatgga ataaacagca ttccaacaat ataggcaaca atagtgatac ttgttttttt
    18721 ggtaaaaatc agtaagcaat agccaatttc aatcattccg agatataaag gtatcgatag
    18781 aatgatttgg gctagtattc ttaaaaatta actagcaaat gtttaaactg atttatttct
    18841 aatctacatg tagtaagtat ttcaattcca ccaattatca ataaaaatac caaacatatc
    18901 cataataatt ttttcttttt catttctttt atattgttat aacagtgaat aacataaaaa
    18961 ttcccgacaa tcattgtaat atttttttgt cacctgtatt agtatttaat ttataggaaa
    19021 ctataaaaac tccctagtga atttacacct ttgggagttc ctgtttggac ggtatgcata
    19081 atacatttaa atcaatttaa cattaacacc tcagcatatg gtatctccac atatatacgg
    19141 tctttctttg gatagtactc aatatttatg ttgatagcac ttccattgtc taatatagct
    19201 ttagcccggt atgtgttatc tccattttca tgagtaatcg tcacatcatc acatgatcga
    19261 tttaagttgt actgttcttg taaaatctgt gttaccaatg gtttcgatga cttctctaat
    19321 ccagatgtac ctgtcatttt tgaaaaaatg atacctataa tagccaagac aacgataact
    19381 ataatgataa acttcttgtt catatttact ttattcaact ttttatctcc tatctccaaa
    19441 ataatttgct cttatccctc ttatcagtac attgacatcc ctaaaagtac accaaagatt
    19501 gctccagcta cccctgctat catcaaaatc gtacccattt gttcatcttt atctcgatat
    19561 aagtagccca taataacagc aacagcacca aatacaacag gaataaatgc taacgctact
    19621 accatagaaa tccaaccaat aatcaaatac agcttccctg tctcttgtcc atgtacgatt
    19681 tgttccttca tttcattaat tgtctgctca gtacttttct ttactgcgtc tgctccagtt
    19741 gtctcatctg tttcttgtag tgctccgcaa aactggcaat acttactttc atctgttatt
    19801 tctttgccac attttctaca atacatgtct tctcctccta aaaacattca attgtttatt
    19861 agttaactgt ttactttatt aaatacatca gaaaacagct cattcattgg tacacttaat
    19921 atctcagaaa gcatcttcat ttgatagggt ttaggaatcg ttctccctgt ttgccacgaa
    19981 cttacacatt gctgtgtaac attcattttt ttgctaactc cgtttgagaa agtccttttt
    20041 cttttctgta tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta
    20101 tatacaacaa tatgatgtat ttcaactcat aaaatataaa aaaacttcaa ataccattac
    20161 aaatacaaca acttgttgta ctatactgag atgggggtgt attaatgaaa cagaaacaat
    20221 atgcaaaggt cattagagaa aagcgaaaag ctctcgggtg gacacaaaaa gaattagctg
    20281 aaaaaatctt ctctacacaa caggcagtag caagatggga aaattctgta acagagccta
    20341 acctcgatag cttaaccgct ctttctagag ccctaggaac ccctgtaagc cattttctag
    20401 acaatgttgt cgtagattat gaggaagagt ttttggctct ctatcgttct cttagcactg
    20461 aagatgcagt tcgaacaatt gactatatga agctattaaa aaggcaagaa aatgaacgca
    20521 atcagctatt gaaagattga aaaataaata acccaccgaa ctttaacggt gggtgtattg
    20581 agatgaaata agattaagag ctatggataa cctgtctagt ttaatacaat tgggtctttt
    20641 ctcttcctta ctctccgtcc atcatctctt gttacacttg taaattgata aagaggaact
    20701 aattcaactc ctgataatat caattctttt attacttcat ctatccctgc tccttttgct
    20761 aggtagacta aaaaactact acttgctttc acattgtata gtctcttaat atccttagaa
    20821 gctcgttcaa gagtgaatcc ttgtttaaca aagtagcctc tgttttcttt taaagcattt
    20881 tccacgctgt tattcccaac attacgtaga cgttcacaaa cagtgttagt aagttctttc
    20941 tctgtcatta gatatgttcc ttgccacgat aagccctgta aaacttcatg tgcttcatct
    21001 aaaatcaatt gtttttcctt gctaagttct gatcgggaat tttgaggaaa tactctttta
    21061 gcctcttttt cgttaaaaca tatcttaacc cattcataag aaaaatattg atatgtaaaa
    21121 tgaaattttt ccagaagtgg aaggatatct tcattcaatt tttgaatcac agaagttgca
    21181 ttaaaaacgt ctaatcgata gacattgctt ctcttatgat taaagtttcc taacgctttc
    21241 tcttccttta acctatttag gtattcgagc atcttcaaag gaacatcttc atcatctaat
    21301 ttttttatca atcctataaa agtcataagt gatagtaact tattacattt atctatgtca
    21361 taagttttac catagtaaaa actcattttt ttagctaatt gttcccccga caaaatacaa
    21421 gtcacacttt ccttactatc atcaaaaaaa ttaacatcag aaagcaagac atcatagaca
    21481 cgtcgcattt cctctagata attccttatt gtcctgaaaa gtttgtcttt cattgattca
    21541 agagtgtcaa aaagtacttt tgattcctct ttcaattttc gaaaatgtgt aggatacaag
    21601 tctaggttct ctaatagcca attaaatgtt tctacccgac tgtctttctt taaaattagc
    21661 atcacaactc caataaaatt ttcactgata tcacaattac tggaatgaca ataaaaaatg
    21721 gaatgcccag ttttactggt aaaaatacta gcagagggat tatgatcgtt gtgaaacaga
    21781 catcttaagt ttttacttga cttaagtaat ttattcatat caatggttaa caatctttca
    21841 taaatctcat taaaagacat gtcggaggaa tcaaacaggc agtctttaaa ccactgtttc
    21901 atttcttctt tgtcgtcatt ttttatcaac ttaacaaaat cggtttcatt gactagttca
    21961 tactctctcc ctgaatcact ttgacaattt ttagaacatg tcatggtaaa atcaatcgga
    22021 agctcagtaa tatctaacat gttgtcttca ttaattatta tcactgcatc tgacttatat
    22081 cctccaaaaa acattctatt cgaagcattt acattgtcat cacacccggg gatgttcttt
    22141 ttcaagtatt cgtaaacagc aaccatttta ccatagtcag taattgcttc ttttaataga
    22201 aagacaatct tgtatttttc tagattttca gagtaccgaa tacttttttg tatcgcaaac
    22261 gcagaattat gaactaaatt gatagataaa accttttcta tgcttaagta tctactatca
    22321 tctttactca aatctactac cttccccttc tcatctttaa cagtgttatc gacatccaga
    22381 ataattactc tggtttccac aaaactctcc ttgtcattgc ctttattctt tgtcttgctt
    22441 ggaataaatg attgtgatga atttagtatc tctacaaact catctcttgt aacttctcta
    22501 tagttatcta aacacctatc tcttacatca gttttttctt tttttgtaac tggcttctct
    22561 aataattttt ctttatctac ataaattgga atattaatca ttgatgtttc catttgtgtt
    22621 tcctccttat aaaattctgt taaag
//
LOCUS       DASNNU010000043        22170 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.043, whole genome shotgun sequence.
ACCESSION   DASNNU010000043 DASNNU010000000
VERSION     DASNNU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.043"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..329)
                     /locus_tag="U8116_001534"
     CDS             complement(<1..329)
                     /locus_tag="U8116_001534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434535.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(343..600)
                     /locus_tag="U8116_001535"
     CDS             complement(343..600)
                     /locus_tag="U8116_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434536.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(795..1337)
                     /locus_tag="U8116_001536"
     CDS             complement(795..1337)
                     /locus_tag="U8116_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434537.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1358..2449)
                     /locus_tag="U8116_001537"
     CDS             complement(1358..2449)
                     /locus_tag="U8116_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN8434538.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2457..4667)
                     /locus_tag="U8116_001538"
     CDS             complement(2457..4667)
                     /locus_tag="U8116_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434539.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4683..7211)
                     /locus_tag="U8116_001539"
     CDS             complement(4683..7211)
                     /locus_tag="U8116_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN8434540.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7263..7673)
                     /locus_tag="U8116_001540"
     CDS             complement(7263..7673)
                     /locus_tag="U8116_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434541.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7686..7904)
                     /locus_tag="U8116_001541"
     CDS             complement(7686..7904)
                     /locus_tag="U8116_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434542.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7901..8893)
                     /locus_tag="U8116_001542"
     CDS             complement(7901..8893)
                     /locus_tag="U8116_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN8434543.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8972..9883)
                     /locus_tag="U8116_001543"
     CDS             complement(8972..9883)
                     /locus_tag="U8116_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN8434544.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9902..10246)
                     /locus_tag="U8116_001544"
     CDS             complement(9902..10246)
                     /locus_tag="U8116_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434545.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10266..10822)
                     /locus_tag="U8116_001545"
                     /pseudo
     CDS             complement(10266..10822)
                     /locus_tag="U8116_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10903..11403)
                     /locus_tag="U8116_001546"
     CDS             complement(10903..11403)
                     /locus_tag="U8116_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434546.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11404..11949)
                     /locus_tag="U8116_001547"
     CDS             complement(11404..11949)
                     /locus_tag="U8116_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434547.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11946..13202)
                     /locus_tag="U8116_001548"
     CDS             complement(11946..13202)
                     /locus_tag="U8116_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN8434548.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13563..13874)
                     /locus_tag="U8116_001549"
     CDS             complement(13563..13874)
                     /locus_tag="U8116_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434549.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13897..15207)
                     /locus_tag="U8116_001550"
     CDS             complement(13897..15207)
                     /locus_tag="U8116_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEN8434550.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15821..16273)
                     /locus_tag="U8116_001551"
     CDS             complement(15821..16273)
                     /locus_tag="U8116_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434551.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16275..16640)
                     /locus_tag="U8116_001552"
     CDS             complement(16275..16640)
                     /locus_tag="U8116_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434552.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16739..21022)
                     /locus_tag="U8116_001553"
     CDS             complement(16739..21022)
                     /locus_tag="U8116_001553"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HEN8434553.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21169..21408)
                     /locus_tag="U8116_001554"
     CDS             complement(21169..21408)
                     /locus_tag="U8116_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434554.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21498..21770)
                     /locus_tag="U8116_001555"
     CDS             complement(21498..21770)
                     /locus_tag="U8116_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434555.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(21853..22104)
                     /locus_tag="U8116_001556"
     CDS             complement(21853..22104)
                     /locus_tag="U8116_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434556.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6478 a         4804 c         3308 g         7580 t
ORIGIN      
        1 gaattcattc cccaaaggcc ttttaagatt gatacagcag tccgttcact tcttttagac
       61 ttgtcataca agtcttttaa atgatcccat atcagactag tttgtctatc tagcttcaat
      121 ccattaaacg ctaatgaacg gttcattttt tctattactt tagataaagc tacagaaatt
      181 tttatacctt cacaacgcgc atcttggatt tcaataatag tatcatcaat tagtttttga
      241 atttcttgcc tatcaacata ttcaatctta gaaagagaat cttttaaagc tactaatgat
      301 tcaacgattt cttttttctc ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc
      361 atgtagagca gagtccattg aaccagtaat tacactatct acaacgccat tccaatttac
      421 tctacatttt ccatttttca gatttgtcgg tccatacgga tagccttttt gacattcaat
      481 gttcggatta taccagatag gattatacca ttcagccgca gaagcagatt cagtcgatac
      541 aaacaatccc acaccaacaa atccaataca catcacacta tagccaatta gttttttcat
      601 ctcgtatcca tctcctaaaa aatagaatag ttcttatagt tattcataag acatattttt
      661 tccgtttttg ttttatcctt tctgtcctat aacaactttt ttcaagtaaa gaatgacata
      721 gtttttctta aaaaaacagt ctgttttttt ctatttcttc ggtgtataat aaatatgagt
      781 gttcattttt tattttaata cgcactccgt acataataaa tcttgatcgc atcaatcttt
      841 tgagcttctt gatctacttt caattgaagt aatacaaatt gagtaacctt attcattgtt
      901 tcttctccaa ataacaaata ttgatccgga ttctctatat cttgtgtcac ctggtaaagt
      961 ttcccagttg ttttcatttg tacatgcggg tctacatcaa tactattgtc tttaatacaa
     1021 cgatctgtca tatagtccct cacagcttga ttgcgtttat aaacatctgt gaaattactc
     1081 cattttctag caaaatcttt taagaaattt tttgtcattt catctatttg catttgttgc
     1141 gctgtctcgt cttctaatga accaatttga ctgttacttt cagtactttg aagagttcca
     1201 cttgtttctt ttactgcttc gattgtttct tctgtcgttc tcaattgtgt tttttgtttt
     1261 tcagaagaaa accaatgaca accagtaaga cctatcaaac ataaccaact aaatactaaa
     1321 attatccctt ttttcacgtt tctagtcact gcctttctta tttcacctta cgaaccagac
     1381 tacgaatttt tgtttgattg tacgtgcgtt gataccgagc cataatccgt ccttgctcgg
     1441 cattctgttc cacactagag aaatgttgtc cgccatatga aacatctgtt ataattccgg
     1501 tgtgaccata gattccagga gacaatgcac cacctgcttg ccaattcacc acatctcctg
     1561 cttgtaaatc acttggtttt gggttcatga ttacttgcca accataggtt gtccagttat
     1621 aatcttcccc tattaacatc gcaaacatgt gtccagaacc cattaactgt gggccaccta
     1681 attgagatac ataccatgcc gtaccaccat aacattcccc attatttacg gatcgtccaa
     1741 tgatccgatc caaaaccttc gcattgcctt tactttttcc tgcaataagc gaagtttttt
     1801 ttgttggtag gctattccaa tgcttgatcg caaaagtatc atattccgtt aagttatagg
     1861 acgttatgat actcattgct cctcgataat acggactccc ttcggtaata cctgctgccg
     1921 catagcctcc acgacctaga atcaataaac aacttccagg atctttattg cccacacatt
     1981 gattaaagtt gctttggctc gccaccataa agccataata gtcataacaa gcttttaagt
     2041 tcggaaactt catatagaaa ccaccttcag aaccaccaat tccacgtgca gaaccttgtg
     2101 ggtagggaca accaggaaac caggtaatcc caaaaaagtt cgtatcgttt cttccagatt
     2161 gtgactgtcc ataagctgat tcatagccta attgagaaag aatcacactc ggtaataact
     2221 gatactttac agctgcacca actgctgctt gcgcatgttc atctgtgatt ggaccacgtc
     2281 ctttaatctc tgaaaaaatc ggtaatcctt cggtatattc cccattaaaa gcgacagcta
     2341 gacctgtagc ttctcctcca tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa
     2401 aaaagggaag aataaagaaa agaagacaac ctagacaccc tttcttcatt tcttcctcat
     2461 cctttcttcg ataatggttt ttggactgtt cgtccttttg gtaaagtaaa atcagttcct
     2521 ggttttgcgt ccaaaatcgg cagtgtttta ggatcctttt ctattttttc cgattgctct
     2581 gttggcttat ctaaagaaga aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt
     2641 ttactttcag acgaacgttg ccccggttgt tgcacagaac gatttcctga tagagtggct
     2701 accgacgaat taggaacaga gtattggttc aataactgct gcattgctgg ataactccca
     2761 tttttcgcct gatttttcac ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat
     2821 tgtggcacgg gtacttttcc agaaggcaac ggaatcgatt gaccaacaaa agcgatacct
     2881 ttatttaaca atggttttct ttgattattc ccttgttgct tactcaattg tgccaatgat
     2941 tgtacctgtt gttttggtct tactgaattc atggacgaat ttggaatcaa accatttgaa
     3001 agaccagaat tccactctgt tttcgatttg gtagaagaca tttcaggctc taccccatga
     3061 taacgaacat gacgaccact ataaggcaag gcttgatttg aacgcccacc tagttcaaag
     3121 ggattaacca cttgcctccc ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt
     3181 tgaaatccat gtatcgctgc agaagcgagt ggattcgttg ctcgatacac attccctgct
     3241 aatcctgcac ctttgattgc caaattttgt ccagcagttg atagttgacc aggcagttgt
     3301 ttcatttgag acaaagaatg acttgctatg tttttcgtgt tgtttatagt ttgatctgca
     3361 gctcctaacc ctagtttctt taataaatct ttccgtttta caatgagtaa aattgatagg
     3421 agtgctttga caaacatatt tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg
     3481 tctactatgt tatagatcaa aaatgccacc aaaataatca cactgtaaaa agatttttga
     3541 aacatcaagc ccaaaaagga ctttaaggta ttcatgagca tataatcaaa tactggaaca
     3601 aaagaagcga gtaatgaaaa aggaatcaca agtaataaac acaacaccaa catttgaaag
     3661 ataaaacgaa tcatgccaat cactacaagt ggtaatccaa tacttagatt aagcaatggc
     3721 accatagaac caacgactgc tttataaaca gctttagttg gttgaatata gacacgataa
     3781 gccttctttt tccctctatc ttctcctgca gcagtggtca cttttgtctc aatatcagat
     3841 aattcttcac ttgacgcttc cttggctaga aattcttgtg gttcaatctc tgcttgatac
     3901 aattcttcta aatttgttgt cccataattt aacaatatat acggatcaac aacagccgct
     3961 ttataataaa gttggcgcac tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca
     4021 ccattgacat cttttgtcgc caattcatct agactatttg tagcagaaaa aataaagcct
     4081 tctacctcca cggacaaatc atttaaccct ttggtaaaag cttctccttt actaggtacg
     4141 ttactaccat agccaaacca aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac
     4201 accatttttg cgtaatgttt ccctctaaat atacgaagag taaatagata taacacatac
     4261 aagacgacaa tccccgcacc aataggatcg tatatttttg aataaatagt atccgaaaaa
     4321 gtgaaaaact ggtgaattaa ctcgttcatt gcagctcctt catagagctt ctcaatgaca
     4381 aaatcaaggc cttgccaaat caatttactt aaactaaaga acatattcgt catcattttt
     4441 agaaaatctg tgccatttgc cccaaataac attttgccta atgaaaattc actatcagat
     4501 aaataagaag aaaaggtatc aatatgaaat ttcccaatct catagatcga ttgatccacc
     4561 tgcttttctg ccgcatacac caatccacca acaaaaagca gtccgaatcc aatcgttaaa
     4621 aaaaacaaaa cgcaccattt cttttcctta tgctttatat aagccattgt ttcacctcat
     4681 ttctattgca tgattaacgt atcctgttct tctacttgag agtgacttgt tttttccgtt
     4741 gtttgtaatg cagcggtcat ttcttcaaac aaactgtgta cattgagttt tcctacacgt
     4801 ccatagggat ctaacatcca acactgtcct ttaatcatgt ggctcatttc agctaagttc
     4861 gcttcattta cttccaaacg taaatgattc aaaatctttt ctcgctcact gtcttcatca
     4921 aaagcaaaga cgacaccaaa attccctttt tctttatctg aacctaagtc accaataaat
     4981 tgtgtatcta acactaattg attcatctgg gaacgcccca cacgtgccat tgctgcaata
     5041 atactacgtc ctgcgttcga attattaaat acccatgctt ctgtaaaata aatcgctgtt
     5101 ttcttactac tatctcgttt tccaaacatt tcacaaaaac gacctaaact aatcatcaaa
     5161 cacaaagact ttcgatccgc atctgtatat agttctggtc ggacattgtc atttggtaat
     5221 ttcaaatctt ggatctctaa aatcgttgtt ttggcattaa agtctaatcc cgggttttct
     5281 ccataagaaa atcctaaacg taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca
     5341 tattctcgaa cctctttttg ttccatttgt tctaaatagt cgaccacatg catcaagcca
     5401 acttgttgtc cggcttcttt ttgaactaaa acttcttgga ttccctttaa taccgcccct
     5461 tttaaaaggt gattggtacc taacggactt aactcatcaa tcatatcctg ggctacttgt
     5521 ttcgcatcta ctcgggataa acaaacaatt ggatctaata caccataatt acggggattt
     5581 gtagcatcta acgtgacata actcattttt tgcaaatgtt ctacaaacaa agggtaatgt
     5641 ttctgataat acgggtcttg aatgagtttc gtaaaccatt tgcgcttttc tcctttggga
     5701 tccacaaata aactttgtac attcataaac gaactataca aaaagagcaa gccaactaaa
     5761 aaggtcttcc ctttaccggt ttttccagtt actgctacat gaggcgcatc aaacaacgca
     5821 cctttgattc cttccgcaat ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa
     5881 attaattctt ctaatggtgt tttttctcct cgagaaccac catcccctaa tgtatcttgc
     5941 cgaccaatat accagccgga ttccatacct aattctgata ctgctgaaaa caaccattcg
     6001 ccaactgctt ctacagttgt tcggtgcttc caatgtaact gccgtcccaa tggttgccct
     6061 aataaacatt tatagaacaa ttctcgttga tcattttttg cacggtggat atggataatc
     6121 gtatgaagtt gttgaataac cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg
     6181 tatacaataa aacatgcgat ccaatcgacg acaggttgcc cattatctac tcgttgcttt
     6241 aaatgtttta ctaatcctcg aacagttaat aagcgttcac taggttcttc atcattttga
     6301 accaattcct tatgctctgt tttgaaccgt tccgatagcc aattcagttt taaaccaata
     6361 cctccctgtc ctttcaagcg tggatagtga attttcattc gcaattctgc tgggaaagaa
     6421 agatcctgag caaattgaaa tagatcacaa tttctcatat tttcaggtgt ttccccaata
     6481 ggcaacaatg ctaaataact actccctaac tcatcttcta attgaagaag gcctttatgt
     6541 tccttcggta tacgtacata cgttccttga ttgtctttca gtacttccca attattttgt
     6601 tcttctactt tgtgagccat tcctcgaata tattgcagac gattgagata caacagctct
     6661 ttttttacta agggacgaat agaaaagcta taaagtaatt gtgtcacttc ttttacataa
     6721 ggatcacttt cagaaaacaa agtttccaaa tcatctactt ctaatccaaa taagcgtgct
     6781 aaacttcctg ctaaatctaa ccagattaga tttattacat catgaaaact ctccaccgtt
     6841 gctttttctt tgactttgat tcctaaaata aatgaaggac gatagacttg accttctcgt
     6901 tccaacaatg tcactgtttc ttttgcataa tacgcaccaa ttgcttgact atcgggagaa
     6961 aatccctttc ctaatgtgtc aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc
     7021 aactccacat cttcataatc tgctaaaagc aacagtaaac gagccatcat ttcttttatt
     7081 tcctgtgtct tttttggtgt gttttttgaa attgttcgtg gaaaaacatg atagtaggcc
     7141 cagatacaat cctctccatt taacaaacaa tttttcttga atgcttttac tggttctttt
     7201 acaagcacca atgaatttca cctcccaata aaaaaggaac aaccagttga ttgtcccttt
     7261 ttctaatcct ctaaataaac atacttattc acaaaaagta attcttccaa tgcattgttt
     7321 gataccttgt cccagttata atacacacaa tcaccattca tcatacgaat catataacaa
     7381 aaccaatccc ataaataaaa atgcactttt tttccatcag gttttagttt tgccagtgct
     7441 aacacacata aataggggag tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa
     7501 atgacccaac caataaatga acgtaaaaat aaatattcaa agattacgac caacaaaaaa
     7561 gttgctgcat tgactaatgc aactggaaat ggaagacgcc aattgcttgt aatctgttgt
     7621 acgcgataag gttccgcata aacaatcgaa taatcataag gttcccaatc cacttcacta
     7681 tcctcctaat ccataaataa tttttcaatg attttaccaa acgcatcaaa tacacgtgtt
     7741 ggatcattta agaagaaata aactgcggca cctgctgcga tagatattaa tgcatttcct
     7801 acttttgatt tcataaaatt tcgcaatccc agataaacaa ccaccattat taaaaaatag
     7861 ccaccttgcg ttaatataaa atctacaatt cccttcagtg tcatttccgt tcttctcctt
     7921 tcctatccaa ccaattatgt gttaattttt caacataata ctgatttgtt tttttcgtaa
     7981 tgacaatcgt catttgttct ttttgtaaaa gtgacgtatc accatctcga aagacgaccg
     8041 ttgcttttac aataaaaggt tttttcgttt ctttataacg caatactttc atctctactt
     8101 gatcaacttt agctgtttga ttgagtcctt caggttcttt catcatgtat gacatatctt
     8161 ttgcattgtt tttcgcatac ttatctaaga aatcttccac aaatccttca acatctttcg
     8221 cttccgacgc atcaaccgtt tcgtataatg tgtcatcata agtaatcatt cgttgttttt
     8281 ctgtattctt tggtagtgcc gtaaaataag gataagcaat aatacgacag cccttgtttg
     8341 aaatagaaaa gggaagattg agtacttgtg tgattgtcac acttgtttct ttcttactct
     8401 ctggattcat cgagcgaata tcataagtta ctttataact agcagttgga atggtttcaa
     8461 tttcttttaa tccaataaac gaagcagcag ttaattttcg aacacctgaa acacttcctg
     8521 gttcttgaat aaagttttgt gcataaaact ctttttttaa tgtttcttga cgttgttgtt
     8581 cttgttcatg attatcgaat gtattcatat acaaagcaac aaaacgattc ataaaggatt
     8641 caatttcagg tgtgtacgtt aattctgacc gactttgtac ttctttcttt tgtaattgtt
     8701 ggatttcatc tttcaaaaac gacacttgaa ccttcgtttt catggattga aaaaatgcca
     8761 agccccctga aaaacaaaga aataaaacca aacaccaaat ccatttcact gctttttttg
     8821 aatggatttt cttttgcttc ggtactttct ctgtataaac tttttctttc cgccgtagtt
     8881 gtcgtttttt cataagctac tccttctagt atgtttttac tagtacgtac gattgacaac
     8941 gataagcttg tttttctgaa aaatagtcca ttcaattacc ctcattttct attccttttc
     9001 tctttctttt atatccaaca aatccagcaa tacttgtcaa cactcctcct agaacagaaa
     9061 gtgcaacatg tatttcttca ccagtctttg gtaagacttt tgtttttcca tctgctgttt
     9121 tgatttcaat attgccactt ggaagtttct ttacaggaat cgacaaatcc tttaccaaat
     9181 tcccttcact atcttggatt aatggaatac catcttttac accaacaatt gtttgatcac
     9241 ctgctacttt taatggggtg gttggtgttg gaatatgggt caattcctct acattactag
     9301 tgacaatcgg tacactcatt ccttgagaag tattcgtcgt aatttcaccg cttggggtta
     9361 ccgatactgt gacttctttt gctggaacaa taggtttatt ttcttttgga attgcttctg
     9421 atttttcaac aggttgtttc ggttcttctt tttgttcttc actggtagac gacatcgttg
     9481 gctgttccgt acttgacgaa tcagtattct tttcagaaga actggttgtt cctgattgtt
     9541 ccggagttgt gactatactt gaatccgttg ttgttgaact atgcacacct tcttcttcga
     9601 tcgaatcagt cggtaaagtt gattcaggaa actctggttc tggtgtcact ggctcttttt
     9661 caggtttctc tgactgcaat tgttcaggtt ctgctggttt ttccgttgtt cctccattta
     9721 gctctgtctc aacatctggt aatccttccc ctggcaatgt ttctacaatt tctgacttag
     9781 gtgtttctac agtttcatct gcataaacca atgaactccc taattgaaac gaaaataatg
     9841 aaattcctag tgttgttacg actgctaatg ttgttttttt catgattatt cctccaattt
     9901 tttatgcaat tcgtgttttg ttcatagacg ccgcactaat attatctgaa cgataaaaga
     9961 actcccgtcc gttttcttct aacccaacat atggattatt tttgaataat tgaatcaata
    10021 gttcactggt gttgtatttt ggatagtttg caaaaaaata atactgtgct tgtcgcccat
    10081 ccgaaaattc aacaagtaat tcataacgat accgcttcaa tccttttcgt tgttttaaag
    10141 gatctctata ccaacttgtc catcgttcca ttcgtggata gagatagaca cgatacacat
    10201 agaacaatgc cataccacat accaccaaaa ataataatcg ccacataaaa attccccctt
    10261 ttcttctacc tttcttgaat catcgaacat tcataccctc tatagagatt tgaaaaatcc
    10321 atcatcaatt gttcatataa acgttgttgc aaatagcgat tgtttcgaat acataattct
    10381 tcatagccat caatcacttg atcttctttt tcatagcatc ttgtagctct atgacgctca
    10441 gcttgcatga cataaccaaa ctctgcacca tatcgttcta ctgttaataa ctgttgacta
    10501 cgcaataatg caatataaca atcacaaaaa tgttcaaccg acatttgatt tttctcacgt
    10561 ttgtgaaata ggtattcttt agaaagcaca ttattcgtta aacaatctga taaatagcga
    10621 taatctggcg gtcggttatt ttcatctgtg aatttttccc ataataaact gatttttatt
    10681 cgttcagcgg ctagatagtc ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt
    10741 ttatcacctg aataaacaaa tccatataat gcgaaacgaa ctcttccttt tcaattggct
    10801 ctcctaaaaa ttccagtgac ataccatctt ctccttccta aaaaaaataa aaaagcagaa
    10861 accgatttgg cttctacttt tctttccttt tatagctctt ttttacggaa gaatccgcaa
    10921 atgacatttt ttcaaaaaat caatcggcac aagttcacga aaacttggag caattttgga
    10981 tagaatcact tccccatctt gtaaaataca atcataaaaa tcatcagtta cttgatactt
    11041 acgcttctta caataattga taaaatagta gcgatcattt tctatttcca ttcgttgttc
    11101 tactaagcgc atacgaacat catcatagaa ataagccaca aatcgccctt caagttcatg
    11161 tttttcaatg ggatactctt ctggtaacaa tctatttttt cgtcgtttat acattgctgg
    11221 ataccttaga agaagttcat ctaaacgtct gtcagtatat ggtaactgac gaatcaacga
    11281 ttctaatcgt tctttataca gatagaataa ttggcgcaag caatagtgta ccgcctctgg
    11341 attttctaat actgttggat cacgtgacca atacgcacca acataaatca taattttttt
    11401 catttagaca atcacaccag caatactaat gaccgatcca caataggtag aacaagtggc
    11461 atagcgacca atttcctcta attctatctc aatctcttct aaagaaagat atgtctgttc
    11521 ttctgttggc tggcgactca tttcccaagt gacagtcacc aatgaaattt cttccataag
    11581 aggtttttcg ctgctaagtg ctgccacaca gttttcatat atcttcattt tatcgcagcc
    11641 ttttttaacc gctaaaatcg caggtattgg caatgaaaat cgataaaagc gaatgaattc
    11701 tagccttcct ttccttcggc taaattccaa ctcttcgagt tcttgttcgt atcccttacg
    11761 aacttctaat aagcgtgcaa tttcttgcca gttctctttt tcctgataac gtccagcttt
    11821 taccgcttgt tcaatataag gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc
    11881 atttaatgct tcgattgcag cctttaaatc caacaggcgc agccattctg ttgtcgtata
    11941 tgcattcatg caattttccc tcctgacgtt cccatccatt ctagatagtt ttgttgctcg
    12001 tcatttagtt cggcattatc aatcccttct tgtacaaagt ctcttcctaa aatacgatct
    12061 gcttcttgta acaactttaa tgtcggagct acttgtttca aaaaccagcg ttctgaacga
    12121 tctaatgaga atttttctgg ctttactgta aaatccaatt cttccatcga acgaaataat
    12181 tttgcccatc gtttatcaac aatccgtgca tcactttcca gtctatccca aacaataagt
    12241 tttgcattaa ttaaaccaac gccaatctct cctaaacgtt tcggtgaatc acctaaaata
    12301 aatgcttcta ctgcttgtgt tgctttctca ttggctaacc gtaactcata tcgattataa
    12361 ataccatatt tcgttaatgc ttctaattct gaaatttttt ctcggttcgc aatttcaaaa
    12421 cgtttctcat aaaaattaaa aaagagagga ctctttcgcg atccaaaata caaagataat
    12481 ccttcacttc gaaaatagcc atcatctaaa aaacaaccgc caatattctg gaatctctta
    12541 aagtccattt ctaataaacc tgctttttgt ttttctagta attcaaagag atcgaaatgt
    12601 tcttccgttt ccaatcccca aagttcatct aacgcaatat cgaatcggct acattgcaca
    12661 cgtgtataac cagcttccaa ctttccttga tttttccaaa tggaatatag ccatgttcgc
    12721 caatctgttt gttgttcacg catgacttcc tcatattccc ggcaaccttg tcctgaaaat
    12781 tgcacaagta cattttctgt tacagttcgt tttgaatgcc agaacaccca gatattccca
    12841 cgaacagaaa gacagtcaaa aagatacaag cccgttggac gtgtttcaaa ttctgtcaga
    12901 tccattcgta atacacgttg aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc
    12961 aaataatcaa tcattgctcg aagatctatt ccgcttttca tatacaatcg aaagcgttcc
    13021 ttgattcgtt gttcaaaatc ctctgggatt tttcttttcc cttgttccat ctttctaatg
    13081 taatcttttg agcagcccat caattgcgca aatcttgctt gtgatagcgg ctttttatct
    13141 ccacttatat tatgaattgc acttaacttc tttctgtagc tttttagctc aatcggtgtc
    13201 attttctcgc tccttttata aaataacaaa atcgattttt tttaaccctt tagaaacacc
    13261 gatatatcaa ctagtccccc tcgttttttg ggaaaagtag tacaactgct gaccttagat
    13321 aatcacttga tacaaaaggg tttataaaac ttttttctat gtgttatgtt atttctgccc
    13381 cccatattat atatcggggg gctttacaca aagaggccgt gcgcaagcgc acgacctctt
    13441 tgtgagctgc acacgctgct agcgagtgcg cattataggc ccatgcttcg cagccttaat
    13501 gcgcaccgcc aggcagagtg cggctaccag actacgtcta gagccctccc gatagataat
    13561 aactacaaaa ttaaatgatc taattttgtt atttcactat atttttcagg agaaaaaacg
    13621 cgataaattg acaaagttaa atcctcttct aaatctactc gataatgata caatcgagtc
    13681 aatcgttcta tatctttttt cgttagcaat acttgattaa taacttttcc tgtaaaaaca
    13741 cgttctttgc gcaaaaagcg gtaatcagtt acttgttcaa tacgattcaa ccaatcataa
    13801 aacgttctgg aagaaagaga aggattaatc attcgtattg ctgtagctac tctataataa
    13861 caactctctg ccatttaact tcacctcctt agttgattac aaaatatgcg catcagaaaa
    13921 acttgttcct tttggatctt ttgctaaata catttggcgc tctagttgtt cttgtaacaa
    13981 ctgattatca tctcccatcg tttgatcttg actatcctct tttactgtaa aatcatagac
    14041 aatcatttca gtaaatttct caaattcttt gataaagtga taatctttgt aaacaaatgg
    14101 cgcaaaaaac tctacaggta catcaaatcc tggaaaagca tatccacgac caggttgatc
    14161 tttgttgtaa aatggcttat ttttaaaatc ttctccaaaa gtcattaaat aaccaatggg
    14221 agacaatcga ccaacagaaa tacggaatgt taaattatcc cgaacaccac ttggaaaata
    14281 ttctgcatct ggtcgttgca ttgctaaaat aatgtacatc cctgcttgac gaccctttaa
    14341 tactaatggc tgaatcaatt tatcgactct atttctcgct tccatatcca acgaaccata
    14401 aaaagctgcc cattcatcaa aaaagataaa atgcggtggt aacccataat aagcataatt
    14461 tttccctggt tcatatttag gatctttcat ggttttcata taggcatacc gttgattcat
    14521 ttcttttaat gcatttcgta aacaaataat catttcttca tttattttgt aaaacacatg
    14581 tcctttgaat acatcgacgt cacctaatgc tgctaaatct gccgcttttc catcgcaaat
    14641 atctactgtc ccaacactta aacaagcttg aattaaagaa tacaataaat aggttttccc
    14701 accaccgatt gcaccagcaa tcaacatatt tggtgattga tcatataccc aatccaaagt
    14761 tttcattaaa tgaatacgtc cattttctgc tatacattga tcaataggaa tccgaccaga
    14821 atctgaaagg aatacataat tgattcgggc tttttctcgt tcttcacgaa ttaaatcacg
    14881 caaaaatact tcagataaca aatgacccat ttttaaaaat cgttcgtgaa aacgcccacc
    14941 atctaaagga aaagaaacca ctaattcttg tttcttttga taaaaataaa cttttggtaa
    15001 tctcattttt tcccttgttt gtttctcact aacttgtttc tttgtataaa caaaccgatt
    15061 gtcaattaac attcgaacaa gtaactgacg ccaataaaca gaggcaaaat atccttcttt
    15121 tagtcgcgac caatgcatca accctaaaat taacccacta accagcaaca aatacaacac
    15181 aatacttatt ccaggaataa gatacatttg ccaattcgaa aacgtactta aacgtggata
    15241 aaatactttc caactaaacc aacctaacag aatcaaaaat ggaaacaaaa agaaaccata
    15301 atagcaaaac cataaataac gattgaaata gcgaattcgc cgaccacgat acttgaaaaa
    15361 atgagctttc aattgtcaca ttcctccttt ctgtttttac ttttcctgtg ctaaaaactt
    15421 ttgacaaaaa gcaactaata caggaatatc acatttagta aaaaaacata atcgaaaaag
    15481 atttaatcga tttttataag ggtgtgcaca catattacat attcgtacta tacgaatcca
    15541 aatcacttcc tgatgatctt gtttccactg gtacggtaat gaaaaacgaa caagatcacg
    15601 taaaaacata cactgcccaa taaccgcaca aatatatgca tttgtttgta caaaaatcgt
    15661 tggattgtct ccaaaatact caatcaattg ttggatattt tgataacaag cacaatctct
    15721 caaagtattt gattggtttt atctgaattc aaaatctccc cttccttcta tcttagataa
    15781 aaagaatagg aagactcctt cctattcttt caaaaaaacc ttattttttc tctttttcct
    15841 tagaatcaac acttggttgt ttttgatcta aacctttttc ttgtgattgt ttcatattag
    15901 gattatttgt tggagcttct actttctcta atcctttcgc ataaatcact aaatctgaat
    15961 attcacgatt gccatcttgt gctggaatat ataccacatg atattctgga tcaattaatt
    16021 tcacaggatc acgtcgttta aaaccagtca aatctacatc tgctggaaat ttaactcgtc
    16081 gtgcaatctt tggttcatct ggatttccaa aaactaattg tgctcgctgt gatcgaattt
    16141 ctcccgtata atcgcctgtt tcattatcaa atacacgatc agactcttct aatgcgccaa
    16201 agcgtacggc tcctaaacaa gtttcatcta atgcttctgt aatgtcataa ttatttggaa
    16261 attttaatcc catcttacaa aacctcccca atttttacac cagttaaaaa gacaccaaca
    16321 tcttgatagc cagttgttcg acgctctgtt tgtcccatat catttgtttc acgactattc
    16381 actcgttctt tacgaaaaac aaaataagct tcttcaaatg aaatacgcac ctctttttct
    16441 ggtaatcgta atcttttatc ctcttcagct gtgagataaa caaaaaaagc aggctgttct
    16501 tctcgcgaac actctgcttg taattgaaca tatcgcccag acacaccatt aatttccttt
    16561 ggtggattag gttcccgtga acccaataaa aatacttgac ctaaaaactc tttcttcata
    16621 cgcaatgctt tttctgccat aaagtttttc ctcctcttaa aaaccaaaat aaattctaaa
    16681 tatttctaca ttacaatcaa acaagcggtt aaaaatccta accgcttgtt cctcatgact
    16741 aatctttggc tttacgtact ttataaagtg ttatacctaa agcaatgact aatactacta
    16801 atccaagaac cataagtata ctacttgttt gttcacctgt ttttggcaac attggtacaa
    16861 cagtagctgg tttaacccgt tgttctggtt tcactggttg cggtggtttt ggcgcttcca
    16921 caatttgaac cgtttgatct ttgtttgtta gatctacatt gtgtttcgcc acttcttcaa
    16981 acgtttcttt ctcatcttcc gttttcgctt ggagccattc cgcaaaactc aagccttttc
    17041 ctgcaagatt gcgaccgtcc actaagaatt caaataagaa ttccatcatt tctttatcgg
    17101 ccgcaaagac tttttccgca tgagtgcctt caaatggtgt gccgtctggt gttaagtagc
    17161 cttgaacaat taagcgatag atttgaccaa tcgcaagacc gtctactttg acccattcaa
    17221 gcacagtttg atttggcaag gccaagatct cttttccgcc atttttattc gtcgctttcg
    17281 tcgtaatggt tggattactg attttgatgg tttggtcatt gtcggtaata tctgcgtgag
    17341 aagccaataa tacgccttct ctaaacagat cttcaaagac cacgacttct ttgcctcgca
    17401 acgcacgtgc gtctagaata aagtctagat tgactgtacc atccgttgtt tctgccacaa
    17461 acgttaaggt acttgtgact tcttttccat ctactagcac tggtttttcc gttactttgt
    17521 ccattagaac accgttgact gtgtatgttt ttccaacgac caaatcgtgg tatttcacag
    17581 cgtctgtaat ggtgactttc gttaatggat taacctcttt caagccagtg ggtttatgtg
    17641 tagccgttgt acgtacactt gggttagtta ctcgtaccgt ttgccctttg tcgttgatgt
    17701 ctttatggtt tgctagttct tcatcatttc taaaaagact ttcaaacacg acgatttcac
    17761 gaccatttat acctgtcaaa tcaaaggtaa atggtacgat caccgtttca ttttctgctt
    17821 tggcaataaa gccaacactc gtttctaccg tatttccatg cacaagcaag ggttcaccgg
    17881 ttgctttatc cattaagatc cctttgacca agtatgcttt tcctggaaga atctcggtaa
    17941 tttcaacggt atcgtttaag gtaaacaatc ctgttgggtc tgcgactgtc aatccgtctt
    18001 caaagacagc cgtggtatga acggttggat tattcacttt cacactttgg gcttcatcgt
    18061 tgatatccgc atgtgctgca acttcaatgc catcacggaa aagtttttca aaaacaacga
    18121 tcgtttgacc tttcagaaca ctagcattaa ccacaaattc aaggtctttt gtgccattga
    18181 ctttgtctgc aataaaggcc aaggtacttg tgatttcttt gccatcttgt aaaattggcg
    18241 cattcgttgc cttgttcatc aatactcctg aaatggtgta ttcttttccg acaaacaagt
    18301 tggtataaga aacactgtct ttaatgacga tcgtttctaa tggatcaacc actttttcac
    18361 catcggcttt atttgacgct tgtgtttcta gttttggtgg aatcaagtaa ttacgtacag
    18421 aaaccactaa cgtttcccca tcatctggcg cattttgttt gaatgcaacc gtgaatgctt
    18481 gtttatctgt atttggttgg aaactttcta atggggcatt ttccaagaag taatagtcat
    18541 cggcaactag acctttcact aaaatttccc cgttttgatc agtggtatat tcaccaattg
    18601 cttgattttt accgttatac aaggtaaagt tcacacctgc gagcggcgtt ttcttcttgt
    18661 tccattcttc atacttgaat aattttacac ttttccgtgc ttgacggttc gcaaaatgga
    18721 tcactttgat cggttctgta tcattggtaa tcgagaacac taatgggttt tgattcaaaa
    18781 cgtaatttac gggggctttg atttcttcca aacgatagcg atcttttcca taagtaagtg
    18841 taccgttagt taggaaaaag cctttttcat tggttttaaa gatatccgtt gtttctgtat
    18901 catttggaat ctccattgtg acataacgat tggctaaagt atcaaaaatc ttgaattctg
    18961 tattagcaag tggaatcact tttcctgttt cttgatccac tttcaccact ttaagacgtt
    19021 gttcaattac tttatcttct aaaacataat ggaaagtttg cccattttca gaaattgtga
    19081 cttcaagtgg ttcaaatagt ttataccctt cttttccttt ggtttcagaa agaatgtacg
    19141 tatcaaagac aacggtattt ttaccaaaag tagcataacc atcattgtct gtcacaacgg
    19201 ttttcaccac tttgccagtt gtttttgacg tcaaagtgat ttcaatgcca gctaattttg
    19261 gtttgttatc tggattcgtt gaatcgacta aatctttatt agcaactttc actaaatcaa
    19321 tggttccttt gatcacttga tcttttgcgg tcgcattgac agtttttcca ggttcaacaa
    19381 caatttcaat tgcttcatca gaaagcacat aaccttttgg cgctttgatt tcttttaaaa
    19441 catatttgcc aggatcaaga tctttaattt ctgcctgtac tttgccgtta atttttttca
    19501 aggtaatttc tttaatcttt ttcccatctg cttgatacaa accatagacc gcattttcta
    19561 aagaagctgc accttgtgct ttcacaccag tttccgcatc ttctttaaac acacgaatgt
    19621 ttccttgttt tagaatttgt aaattaataa cagacgcatt aggttctgtt ggatgtaaca
    19681 caccaattgt ttgagctcct ggacgacgat aggctaatgg cgcaccttga actttaattt
    19741 tattcaattg aatttttgct ttatccggtg cctcttttgt gactgtaatc catacttcat
    19801 tgccgtgaat agatacattg acaccttttg gtttttgaat gtctttcaca tatttcaaaa
    19861 aatcattttt attctttact ttcaacgttt cgccaatttt taccgtatgt gtttgattat
    19921 taaaagttgc tttttcacca aagttattaa tcaaacgttg aatttctgct tttcgacgat
    19981 cataatcaac acggatattt ttgattggga tttttgtgcc caaagcttcc caaatcataa
    20041 attgtgccgc aatgtattgt tcatcggatt tgtctttatt tgtgacatat cctaaataag
    20101 agatcaagct tgtttttttc cgtacatttg aatttttcaa atagtttttc aaagcagtag
    20161 ctgtataaac ttgaccttct gcggctgaga caccaggctc aatacaaaac gccacttgtc
    20221 cattaatctc aaaacgaacc atataattta aagtgaagtt tagattgttc ggactagaag
    20281 taacaccaat attttcgccc caatcaattt tccgaccacc taaaacagct gcattggttg
    20341 cacggcgaac acgaaccgaa cgattggttg gtactgttac taacagtgta ggtgtacctg
    20401 tttttggaat gttctgctct tttttctttt gattttcaat actagccgta gattctttgt
    20461 ttggtttcgt tggttgattt tgactagctg cggttgaaga gattgttttt tcaacgtttt
    20521 cctcattttt tccttctttg tcagcagtag ccgctggttg ttccactgtt tcttctattc
    20581 gtttcttttc ttccgcttgt ttctcagaag actctggctg cacagaaggc gttgcttcgg
    20641 cagctttttc tccttctaga gtggacgttt ctgaagcagg ggttgtaatt gaggcagttt
    20701 cttcaactgg ttgtgaagta ccaggactat tccctgggtg ttgtgtttcc ccaatcgtct
    20761 gatcgagagt cgttttggaa ttgaccgttg gcgcttctgt cacaggggcc tgttcaacag
    20821 tggcttcacc agctgggacc ccggcttctg ctggttggac ttctgtcact tcagaagcga
    20881 cttgggcaat ggcttccatc gtaggttgta tagccgtcgt ttcttctgca tgtgcttgag
    20941 cagttcccac caagaaacag aattgagaaa aagccaccaa tgcgagtgct ttcaaccaat
    21001 tcattttcgt ttttgcatac acaagaattc ctccttttcc tgagaatttt atataataaa
    21061 acaaaacgcc gttaccaaaa gtgataacga cgtgaactgt ctcttctatt tttatctggc
    21121 gctcttatct gaacacattt catcaatgat tttaccctcc cactataatt acagaacaaa
    21181 aaaagaacct gcttcgtgga aatcaactgt ccatttccca attggaatac cagctttttt
    21241 ggcaacttca ccatcaccaa atctcatgcc ataccaatta ctaccttcat ccttaatttc
    21301 tttccaacca tatgacatag cttcatctaa tgaatcaaaa atcatgacac tattgccaat
    21361 tgttacagga tctggcttaa gatcttctgg aagagaaata tcaatcatac cataatgatc
    21421 cgattcctca gtattattgt cattactagc tactggtttg gttgttctac tggcttattt
    21481 tgatacaaca agtactctta ttcttctttt acttctccgt tttcaatttc ccattcaaac
    21541 cactccgcaa agtcttgctt aaaaccatct actcgatacc tattttgtaa ataggcatct
    21601 tctgacgtcg taggatctaa ataggtaggt ttattctctg gacgagcata gacacctacc
    21661 catgtttcgt aatctccttc aaaataacta tctaccaaat agcctttttt tctaagaatc
    21721 tcccgaactt gatttttaat tattgatact tgttcaaaac catcactcat tctatcacct
    21781 caaataactc tagattctca taaatattcc cgacaatttc aatcctatca atgatctcta
    21841 acaaatcgct acttatgctt ccccattcgt ataagaattt cccttccgca aaataaattt
    21901 tccataaact tcttcatgat cgtcccaacc aatatcactc tcgaaaaatt ccacgttgtt
    21961 cttatctttt agctcagtaa atgccataag ttcaacatgt tttccaacct cgaaacaatc
    22021 acaccaccca tcaaccgtta cacgtgtgcc ttcaatacct aagaaacttt gtcctcgtgt
    22081 attaaacact aaaactttcc gcattttttg ctcattaatt aatcacgcgc gaaatctagg
    22141 aatcacaact tattcgcttc ttctttataa
//
LOCUS       DASNNU010000044        21539 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.044, whole genome shotgun sequence.
ACCESSION   DASNNU010000044 DASNNU010000000
VERSION     DASNNU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.044"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..398)
                     /locus_tag="U8116_001557"
     CDS             complement(<1..398)
                     /locus_tag="U8116_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434557.1"
                     /translation="MALERVTSVSPSTSAVVQRSNQSSASAGTQTVQQSPSRGIDRST
                     VTPARRSEPKVGSIKQPGQPTNHRSVTSQKPAEKSTAPTYHIPTVESKTKELSTPKEL
                     ATKYLKESSVTPENEYYDIYQSMNKVNADD"
     gene            complement(2423..2896)
                     /locus_tag="U8116_001558"
     CDS             complement(2423..2896)
                     /locus_tag="U8116_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016612348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434558.1"
                     /translation="MSGFGLLLGLIGVFFLSGCSNSPEKKLSDIFEEGKSFYLSNSPS
                     DEPEFKIDKMKDGSLTVSNIDKGISETMNYEVEEQEKGLYHYKIANSENYGIFEVLDN
                     FISDTKDFNVFYDEENEGYAFVPISKNYSKMDSSLKETREIYSTDPHYYVAEYKE"
     gene            complement(2956..5586)
                     /locus_tag="U8116_001559"
     CDS             complement(2956..5586)
                     /locus_tag="U8116_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEN8434559.1"
                     /translation="MRVRSKKRSFFYRLFSVFGLKKIALFIGRGYSIKGEGLAEEHKR
                     HAPITFLGFFSAFFLVYFTSNYLINFIVSFLPILGKAFTLSINQDFSTAGKLFQTFPY
                     FSRSLFIHVGTNKQLLITLPLAFFFAWFVFKKLTRKFDSLSYGQEGDARLATIQEIKE
                     QYPAVPDRKERFEGIGGIPITHYMNKFYIDRNTVNSLILGTSRSGKGESIVFPLTDIL
                     SRAKEQCSLIFNDPKGELYKGSKETLEERGYEVQVLNLETPDQSMSYNPLQLIIDAYV
                     RGDSSSAQMLVDQFTYALFNDPNAGQNKWVYSGAQKLLNGVILALIDYYLEKHDLGKI
                     TMYNVAQWIIEMTNRKVIDRVTKKEYTQLDLYFERLPQGHIAKAQYSSFSSMDSKNKG
                     PIQSAVLDKLTIFQGFEAIAKMTSKNSFRLKSVGFPKSLFFQFDRSMYDQILKVRFLR
                     NGQVLGVETIRPNMAGVVDLNFNLNLQTGDQLEVVFQDKKATYSITLLEKDGELNRIS
                     GDLDELKQCELFYTEKPIAVFMITPDYDPSKNVISSMFVKQLYTTLAQNAGNTRGGKC
                     FRRVHFILDEFGNMPAIESMDNIVTVCLGRNILFDLFLQSYAQLGSVYGDYTAKIIKE
                     NCQNHLYIMSQDKDTHEEISKRVGEKTVEKHSTGEQDPLSLDKSKQTNVGSQRIITPY
                     KLSTLMMGEMVVLRLHRENKKRQKIRSYSIFSTKETSMPFRFEYLGEYFDPNRPIEDF
                     NIPSEHKTFDLAANAIDVDDLISESINRSLLLSLEEGLQSEEELLQIDEETFASVLKR
                     FDTKDLENYREIIKVSIVEDPEESQKIDLIDFLKQAVTEDLKPLFNEEERCAFLQLAN
                     FYLNKPYYKDISNLVRLDTE"
     gene            complement(5598..7868)
                     /locus_tag="U8116_001560"
     CDS             complement(5598..7868)
                     /locus_tag="U8116_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434560.1"
                     /translation="MGNYILRFTKLPKEIKATKRLTLKKKYSLPEARELFYQVQADVG
                     PLDNKMTLEIHRIEGTKDYWEFTLPLSKDVPLDLLIDEDIIQELRNLKGSQKEQFEEL
                     ENDLLVEFDQSFEPVEQEVNEPKPSFLSGLSLPEVKLPEVAENLLSRVLPKKQQVAVP
                     LIEESPSENFVEEALELPEDNYVPDYETVEEEDGVPLEDLEMEKEDILSPQASQDDIV
                     PSTVKESIPSVVIPPLDAYLDLTTQPIFNPIESLIAKLEAKLDRQTQELIVYFDLADK
                     NDFISERKKEFIRTQYNPTFYKQLISSLYKLKNDVENKLIKKLTIAYQEITADDGSEE
                     CLAELAAKKAILSEEFTDKIEAAFTSLENQLALDKARIQKRQQEELERLNKRHQDELY
                     QIDVKLQADKQEKAAQFDQMRQEAIEQEEEKITASYEEKAHKSQYSRLIERKNSQLEE
                     LSTILVESVQQVTDQELQYLQSMKSKLIESQPVFEEQRRIHEQELREKAVLAQRDREL
                     AIKEKELSLKDQAVQNVESTKQQNQQKEMEISQILAKLIDDHTRRGAENIATQQQLMA
                     FIQQQQQGTPLQAAPTQSSAAQQVAGKPSLWKKITLSGAVLLAVAGFGGYVHAKAQQD
                     SNQTTVSAAVQTENSYASFESTEPAVPSYDDQISAGKYIEAAKEYPENIDHVVQVVFE
                     KEDTDTLKQLTKLGESDYGLLDLAILQQDQKAITSIYQELSDALKKDLTMGQKQQIAS
                     AYLLQDKPKEAKSVLE"
     gene            complement(7932..8225)
                     /locus_tag="U8116_001561"
     CDS             complement(7932..8225)
                     /locus_tag="U8116_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434561.1"
                     /translation="MEEINMLMKLQLALLTLPTNDWVSELDKAGTTIYSALQRLSFPV
                     AVITTIVAGIMFMSSDRKADVAKSIWFRVAVGMIVIMSAVSLATYFQGIGKKF"
     gene            complement(8164..8457)
                     /locus_tag="U8116_001562"
     CDS             complement(8164..8457)
                     /locus_tag="U8116_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HEN8434562.1"
                     /translation="MAGKKSAVSPFVEAAKELLSQEGITFKEWTNDHLRDKKMAILEG
                     KDKEWEESTIEKQCESYVLSKINTLVSDKNQVKNGGNKHVNETTTSTTNATNE"
     gene            complement(8484..8894)
                     /locus_tag="U8116_001563"
     CDS             complement(8484..8894)
                     /locus_tag="U8116_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434563.1"
                     /translation="MSGQYRVHFSHVNKKLTRKYRALNTKMVHGIFQAQQIMEQLQRS
                     ITEPTKLSVVIDNGEQEIILEDVVVDPTRNTVQLLDMLQEEISTLESNRLEGEKLYQE
                     INRQFNEERKALPEMEPRQKRTYSKKSRGFSFFR"
     gene            complement(9056..9424)
                     /locus_tag="U8116_001564"
     CDS             complement(9056..9424)
                     /locus_tag="U8116_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434564.1"
                     /translation="MDVEDSQGEVFSMLFYCLTVLAFLSVVVFGYQLQTITTYRQQVN
                     YQIERKGGLTAEAVSELKDYSNKQFHGWYTVESPSLGKKAAFGDTVAYTIKATYPVLF
                     LPDNISVKIGIEGQAPSQVR"
     gene            complement(9442..10206)
                     /locus_tag="U8116_001565"
     CDS             complement(9442..10206)
                     /locus_tag="U8116_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434565.1"
                     /translation="MNLVIRNKTTEHSENRPFFSRLLPDLVYEDLLEMNNTKENIEKW
                     SKKNARNFGICFSFFIALGLLLSLYLIPIGFVVSVLVYLNTIKSVKIVYQQFRFERQI
                     QFSKFSRLLVPVLKQTRGGINLRSVFERIVPRMPHEIDKKILQRLMLDMSRNPSSFDP
                     FKMFAKQMSGTDSSRLFMVTLARLAQGSANLQVIDQLSRITSEEMMAGIEQIIHFKIS
                     RFSGLYMKLVGAILVFVLGASIAIIPFQLGKVYVYL"
     gene            complement(10203..11060)
                     /locus_tag="U8116_001566"
     CDS             complement(10203..11060)
                     /locus_tag="U8116_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434566.1"
                     /translation="MRINQLQTEAKNRVNLEELHALNSAYGQRLKQKEIIQLVGLPAV
                     VLGVFSLILTYIWWIVVGCTVIGAIYGYRFILPKVVNRRYQMQSLVARNKFLLNLTQV
                     TMDNDLTVLQSLAYVIDETEGELSEDLRSLYAALGVGSDPERVSYLLNNLSEKYHDDA
                     IFGQYLEQLDTIIQEGKNNTDSLQYLTEHHSKMLEKVNLFLKKKNEMLSGVQTLLILM
                     GAIILLFHGVSFFMNGDFAQYMDGYAHHPIGYVSSAICLTSLSMILNSFFKQYFDDSV
                     TELGVRTKK"
     gene            complement(11029..12492)
                     /gene="tadA"
                     /locus_tag="U8116_001567"
     CDS             complement(11029..12492)
                     /gene="tadA"
                     /locus_tag="U8116_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016612357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEN8434567.1"
                     /translation="MVVIREKKSIFKDEKDVLRNVRNIVTTNQFRHIPSVTEEERDDY
                     LEKLVEDSRVEVIRDDLVEHYPDEVMRAFTSKASREKLTKLVQEEQRFGKDDEGQKKA
                     AYVVNEIVGFEFIDRLLLHDESITDIGWNSTFLTVESADQLRIYTAEQLQLTDPEKTV
                     FRIVKKFADAVGKPFTRNDPILDSVSGNLRLNAMHQVISPTGTTMSLRVSRPGLALTE
                     ETFVDFAPSFVLEFLKIIMKAGSMSVIGGETGSGKTELIKMMLSFVPKKDRIFMIEDV
                     PETRIKELLPDHDIFSLVTGNIVTISDLVQAALRNYPKWIVPSETRGSEAYEMIQAAR
                     TNHSGLTSIHSTNCRAHPRRLVTMSSMGYKINEEAVTEDILTFFDFGFQIKTAYIEID
                     SENLIFKKIRYLSEIVEYSVKDTATTLFKQVLNNGQFYVTTGQLSDQYKEKLAERFLS
                     FDFPEMTNEKINKSEELEQILQKAVAEFANQSTSDGS"
     gene            complement(12497..12883)
                     /locus_tag="U8116_001568"
     CDS             complement(12497..12883)
                     /locus_tag="U8116_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016612358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434568.1"
                     /translation="MTHYWRQITVMIVGALFMILIAWFFIGNAKKDTEAFNITEALRT
                     AAISNKDDSARIQPGAFYLDQASFEKEFEQTIKKQPFYAKKTVKVTYNYLKEADNKGI
                     KGIRAYVESDGSIDEATCILSTPEGG"
     gene            complement(12951..13112)
                     /locus_tag="U8116_001569"
     CDS             complement(12951..13112)
                     /locus_tag="U8116_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434569.1"
                     /translation="MDTVQNHSHTLMNVAIAVGLVAIVGGVVFKFFPDFTTQMLAKIT
                     SYVSTLPTK"
     gene            complement(13164..13484)
                     /locus_tag="U8116_001570"
     CDS             complement(13164..13484)
                     /locus_tag="U8116_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434570.1"
                     /translation="MYGSAVFDVRSELKQSRHLLRKVVFSSLGILVFFFLFKQLIGIP
                     FNYLNQISSSFLNFGSGSSNVSGSLTSAIQQNQFVQAVMVFVTGILAVCTKFFDEIKQ
                     MFFK"
     gene            complement(13498..17124)
                     /locus_tag="U8116_001571"
     CDS             complement(13498..17124)
                     /locus_tag="U8116_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016612360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434571.1"
                     /translation="MKKKPKFIVSFSVMIISLLAMANFPYVLAQEQTNTAKTDQEVAA
                     VSEETLNKEQESTVVQGADGLLYVVADKSDKEQLISSDTPQIAEKTEGDFTAVEVGID
                     EKRQEKADQADQEIKDFDEIDLNDIESLQSAVNSEDLPFIEEDQVAEELAKTKNTEES
                     EQVLMQTRFFYAEEILNQTNNLNDVPSMLKESKVALLASVSKTVSETTINPPSGYPWL
                     MYIVRSNGYSYTEDRGIRRTIDGKDAFCLQAGVTAAKTYTDAGSSYLSTSQKETITSI
                     ISAYRELGGWANNDAGKQRWMAAQILVWENSNEKPKSITITPNHKAGVTDWKSKMTTR
                     AGQIRTRPQWDATAKTVYEGEKATFTVKNSWGDHNLYVESVSGGAKATISGNKLTVDT
                     NGSTATKITVNMLKGSKTNEKYPTTVWANGSYQKLVTGSFYVSTSVTVNVKRKGTVDV
                     LKVDADTKKPLANATFRFTYDGKTTDVKSGTDGKAKLGERLKAGSVVKVKEITAPSGY
                     KLNGTEFSITVGENTNVSKTWTNERLAGAVELTKIADVDQKSLDGVVFTLYKSDGSIL
                     QDNLKTDTNGQLKVKDLSLGSYYFKEKASLVGFETDSKNYSFTITADVTEAKPVKLTI
                     TNQYKLSASKTVSDKNETGKTSNTWSAASDDFLQYDLTTSTVEKLGSRYLADFVIEDQ
                     FPAETVAISTVQVLAQGSDVTDQFTITKDLTNGKVIATAKSTALTTSGFYGKSYDLRT
                     KMMLKQEVLTAKNKATLSFSKQGSVKTGTKTASSNAVTTTLPAFKLTVNHVTETEKAL
                     LKQVVETKYDGESYEYKPLDDLFDVKGNRYRSKVTRSGVVAGKDLVLEIPYYLPSLEI
                     NAEKVSILTGKASLTDSLKATITFSKSASYEEELKNVLYRLTVTDQKQKKTVFTKDIS
                     LGSYASSLVVDLPTAGFTKGEKVPYSLSLQVLKNPDKVKAETDTIDFSTFGYTASEKV
                     LTNADLKNDKLSYGAVSQTEATRKTKAYVEKKETLQFDFEEQRSSKTGYGLPITLEPI
                     YSSEIQDTPLLSFTLSSPTKLVDTYLDLQYVESKGNIQIPLDRTVNQVAKQTTAAITS
                     TKFEFPQISVEEKTGALYTQEQVKNPTKKIPNKLLDGGRKFYLPIWAEKGDYNLTLQS
                     NVIGRNFIQVSMNQTLSVYANMYATIDSKTKKEDELLLTPVYPDTTVPKGWSKAEITW
                     LRGK"
     gene            complement(17217..17666)
                     /locus_tag="U8116_001572"
     CDS             complement(17217..17666)
                     /locus_tag="U8116_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016612361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434572.1"
                     /translation="MKGGQKQMDAEKKTLYLTVGKEVSLSFTGNGEALQYIRLSVNKL
                     AEIINNGLVDRQSIFEIDEVSLITKSNYKTVVQVVAGKQVLHGNTDHVDVVIDKDKTK
                     QKAAGKDIFTNGDFLFIVDQEQHISKQELHTLNIKNSKSYLNEGDRL"
     gene            complement(17905..18279)
                     /locus_tag="U8116_001573"
     CDS             complement(17905..18279)
                     /locus_tag="U8116_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434573.1"
                     /translation="MSSIVNMIVPFTICLYGLYLYGKMSNLEDIDQYLSKENQEYLLK
                     NCYYDHSFKKDTLQEIERMIRRITAQLTDLNEDRLIIRAELSSKIDSLKALKHKILVD
                     SYNEKLVELSPDQRALDDWNRF"
     gene            18421..18627
                     /locus_tag="U8116_001574"
     CDS             18421..18627
                     /locus_tag="U8116_001574"
                     /inference="COORDINATES: protein motif:HMM:NF025005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEN8434574.1"
                     /translation="MKMTLVRREQFTAIGYDPILKELRIEFKIGIYTYTEIPEHIYDA
                     FIHAHSKTFFYNRHIKNYPCRKGF"
     gene            complement(18666..19013)
                     /locus_tag="U8116_001575"
     CDS             complement(18666..19013)
                     /locus_tag="U8116_001575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434575.1"
                     /translation="MGMILNHVTLVYDYDERLQELRNKAIEMFDPLMHERVQTMKTDG
                     EFVSPIISSLSNGEEFFIINTDGSKVGWETNSAFQKVRKEFVQYCRDYGAMQVATIEF
                     GENDKPILTDLVY"
     gene            complement(19074..19268)
                     /locus_tag="U8116_001576"
     CDS             complement(19074..19268)
                     /locus_tag="U8116_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434576.1"
                     /translation="MMNAEERVWERKEETLTRYRSFEKGLAVDTGYDAKKLAKVCEEE
                     GTTLEKFSEYAQKRVGTYKQ"
     gene            complement(19307..19693)
                     /locus_tag="U8116_001577"
     CDS             complement(19307..19693)
                     /locus_tag="U8116_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434577.1"
                     /translation="MMNKETFFLGKTNVTIENPVSYYPETVNEEEIQHFLEKSSESYD
                     NSFRELAEASSAFYELEKKLNSLEVQKEKVNHYLEVTAIKEIVKDTGISRTTIADLKS
                     GKRTLETTRLGIFETLVKYSDGLEKA"
     gene            complement(20204..20362)
                     /locus_tag="U8116_001578"
     CDS             complement(20204..20362)
                     /locus_tag="U8116_001578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434578.1"
                     /translation="MTKTNKLLAVGKFFLKMMFKVSMGLLKVAVFIFIFTFTFATINA
                     TSTLFSKR"
     gene            complement(20734..21135)
                     /locus_tag="U8116_001579"
     CDS             complement(20734..21135)
                     /locus_tag="U8116_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434579.1"
                     /translation="MTKGLAVYVKDLARIKTSVQLFNTQHWMIEGENAYRYTTLFEKG
                     KPKKIFFGTAKTETIFPQIKLLFDQKERVIAQRLNSNGRLITTRENKYFKVRNHRAER
                     ALEVYALITAIEEKEYEEMIQNYWYQERKRA"
BASE COUNT         5925 a         4595 c         3743 g         7276 t
ORIGIN      
        1 taatcatcgg catttacttt attcatggat tgatagatat cataatactc attttctggt
       61 gtcacactcg actctttcaa gtatttggtg gccaattctt ttggtgtaga aagctccttc
      121 gtttttgatt caaccgttgg aatatgatac gttggtgccg ttgatttttc tgcaggtttt
      181 tggctagtca ccgatcgatg gttcgttggt tgtcctggtt gtttgattga acctactttt
      241 ggctcactgc gtctagcagg tgtcaccgtt gatcgatcaa ttcctctact tggtgattgt
      301 tgcaccgttt gagtgccagc actggcagac gattgattgc ttcgttgtac gactgcactt
      361 gtcgaaggag acacacttgt gacccgctca agagccacag aagctccttc agacgcttgt
      421 tcactcgatt gaacagattg actaggtgct actgcagaag tgccgtaaga cgtttctggt
      481 gttgctcgtt tcacttcctg atcattggct ctttgattgc tatccggatt tccttcagat
      541 gcactcggat tgtttgactg ttgcgtttta ttcccctgat tgactttgtc agtcgctgct
      601 tggatggcag cttttctact tggcgtatga atcggtggaa tggtcgttcg ttggattttt
      661 tgtgaagcgt ccgttttccc cgttgccaaa ccattcgcac gattgccgtt gatagggtcg
      721 atcgttgatt cagttcgatt gccactttca cccatatgtt tcaacgcttc agctgaaaca
      781 ttgccaatgc tttttccatc tggttctttt ccttgagaaa cattgctgga agaagcagaa
      841 gcgttcattg gttgatacgt tgtttctggt gaaagattgg ttcctgaagg cagctctcca
      901 ttagcagaaa cctgatcctt gtcttgaacc gatggattta tctgatcatt tgaaaccgct
      961 gatcgattcg atccttgcgt ttgtgtcacc ggctcttgtt ggtcttgcat ggcaaacgac
     1021 tgtcgcatgt tcgctgcaaa ttcttttata cttttagtgg aagatccatt gtttcctgga
     1081 atagttgagc gactctgaat cgatttatca ttgttatttt cgtgtacacc gttattgact
     1141 ctcatacggc tgactgcatt tccggcgtgc tctttaatac tactaccagc atttactcca
     1201 ccttttacag cgcttgtcgc agctttcgca gcaactgctg taccaaccat agcttgccat
     1261 cctgagcgaa gcccgatatc caaccccaga aatttcccaa agaaatttgg gccgtcgatc
     1321 gtaattcgga caccaacaat acaaagtact aaatatggaa gaaaatcgac atcaagatta
     1381 cctataaatg aaaagtataa cttaaagata ctcaagctaa accctgtcaa cataatcgct
     1441 aataagctgt tcccaatatc caaaagaatt ttttttgttc gttgtccggt ttcaatatca
     1501 gaaacagaaa gaactggaaa catcaacttt gcaaatagta gctcaatcaa agacgttgca
     1561 attataaatg cagagcagat aaaagcaaac gacactgtca gcaatgacaa agtgattgtg
     1621 cccgtcctgt atgaaaaacg aaatttacct tctttaaaaa aatcgaacat tccattttct
     1681 attttttctg atatgacttt tccatcttcg tcagtcttta gcctatattc taggtaagaa
     1741 gtatcatctt gatttttagc tttatccttc atttttctga ttttttcagg agttaaaact
     1801 tctgataaat cagtagcttt aaaagcttgt tctgacaaat tgtttttttg agttcccgca
     1861 tcactattca gtcgagaaaa accgtttttc gaagaatact gcaagtctga taaattttct
     1921 tttacaatac tgtaggacag tgtacttttc tcagtcgtat ctaaattttt tgtttctcca
     1981 taaacttttt gagaaagctc cacacttgat gtaatcaagc ctggaagctg aacacctaaa
     2041 caaacaaaaa agacagccct gaaaaggcct gcctttagat ccactcgttc gtttaaaata
     2101 tatttgtaag caataacaat aatcataata atcaaaatag tgaatgagac taatcgcatg
     2161 ctggtatata ttttaccgat tgtcccatta tcaagaaatc caaagacctt gaacacttga
     2221 tccaagatat cttccacccc agaagctagt ttgtatagtc cttttaccca gtttgcactt
     2281 atccatttaa ataaatagct aaatagagtt gcatcttgaa tccaatcact aaatttttga
     2341 agaaaatccg ttgctttttc cgttttactc accttccaat cataaaaaaa gaagccttat
     2401 tcaggctcct cgtagtgact atctattctt tatattctgc aacataatag tgagggtctg
     2461 tagaatagat ttctctagtt tctttcaaag aagaatccat tttcgaataa ttttttgaaa
     2521 taggtacaaa ggcatatcct tcattctctt cgtcatagaa tacattaaag tcctttgtat
     2581 ctgatataaa attgtccaaa acttcaaata ttccataatt ctcactattt gctattttgt
     2641 agtgatataa acctttttct tgctcttcaa cctcataatt cattgtttca gaaattccct
     2701 tatcaatatt ggaaacagta agagatccat ccttcatttt atctatttta aattctggtt
     2761 catctgaagg cgaattagat aaatagaaag attttccctc ttcaaaaata tctgacagct
     2821 ttttttctgg tgaatttgag catccactta gaaaaaaaac accaataagt cccaacaata
     2881 aaccgaaacc actcattttt aaacgtttca cattaacccc tctttttcta ttcatttgat
     2941 tttagtatag cacgtctact ctgtatctaa tcggactaaa ttagaaatat ctttatagta
     3001 tggcttgttt aaatagaagt tcgccagctg taaaaacgca caccgctctt cttcattaaa
     3061 aagtggttta agatcttccg ttaccgcttg tttcaaaaag tcgattagat caattttttg
     3121 tgattcttcc ggatcctcga caatcgaaac tttgataatt tctcgatagt tttccaaatc
     3181 ttttgtatca aaacgtttga ggactgatgc aaaggtttct tcatcaatct gtaacagttc
     3241 ctcttctgac tgaaggcctt cttccagaga caagagcagc gatcgattga ttgattctga
     3301 gatcaaatca tcaacgtcga ttgcgtttgc tgctaaatca aaggttttat gttcagaggg
     3361 aatgttaaag tcttcaatcg gtcggtttgg gtcaaagtac tcacccaaat attcaaaccg
     3421 gaaaggcata gaagtctctt tcgtagaaaa aatagaatac gaccggattt tttggcgctt
     3481 tttgttttcc ctgtgcaacc tcagtacaac catttccccc atcataagcg tagataattt
     3541 ataaggggtg attattcgtt gagagccgac gttagtttgt ttgctcttat caagagaaag
     3601 tggatcttgt tcaccagtcg aatgtttttc caccgttttc tctccaactc gttttgatat
     3661 ttcttcgtgc gtatctttat cttggctcat gatatacaag tggttttggc aattttcttt
     3721 gatgatcttc gccgtatagt ccccgtatac agagcctaat tgcgcatagc tttgtaaaaa
     3781 tagatcgaac agaatgtttc gccctaaaca aacggtcaca atgttatcca tactttcaat
     3841 ggctggcatg ttcccaaatt catccaggat aaaatggact cttcggaaac acttcccacc
     3901 tctcgtattt cctgcatttt gtgcaagtgt tgtatacagt tgtttaacaa acatggaaga
     3961 aatcacattt ttagacggat cataatccgg cgtgatcatg aatacagcga tcggcttttc
     4021 tgtgtagaaa agttcacatt gcttcaactc gtctaaatct ccgctgattc gatttaactc
     4081 accatctttt tcaagtagag tgatcgaata ggttgctttt ttatcttgga aaacaacttc
     4141 taattgatcg ccagtttgta agttcaaatt gaagttcaaa tccaccacac cagccatatt
     4201 cggacggatc gtttctactc ctaatacttg tccattgcgc aagaagcgaa ctttcaaaat
     4261 ttgatcatac atcgatcgat caaattggaa gaacaacgac ttaggaaaac ccaccgactt
     4321 cagccgaaaa ctattttttg acgtcatctt cgcaatcgct tcaaaccctt ggaaaatcgt
     4381 taacttatcc aagacggcac tttgaattgg ccctttattc ttcgaatcca tagaagaaaa
     4441 tgaagagtat tgtgctttgg cgatatgtcc ttgcggcaaa cgttcaaagt agagatcgag
     4501 ttgtgtgtac tcttttttag tgacccgatc aatgaccttt cgatttgtca tttcaatgat
     4561 ccactgtgcc acattgtaca ttgtgatctt tcctaaatcg tgtttttcta aatagtagtc
     4621 gattagggct aagatcacgc cgttcaacag tttctgcgca ccgctataga cccatttatt
     4681 ctgaccagcg tttggatcat tgaacaatgc atacgtgaat tgatccacca acatctgcgc
     4741 agaagaagaa tcccctctta cataagcatc aataatcagc tgcagcggat tataactcat
     4801 agattgatcg ggtgtttcta aattaagtac ttgtacctca tagccccttt cttctaatgt
     4861 ttcttttgat cctttataaa gttctccttt cgggtcattg aaaataagag aacactgttc
     4921 cttcgctcga ctcaagatat cagtcagtgg aaaaacgatc gactcaccct tacctgatcg
     4981 actcgtccct agaataagtg aattgactgt attgcgatcg atataaaact tgttcatata
     5041 gtgcgtgata ggaataccgc cgatcccttc aaaccgttct tttcgatcag gtactgccgg
     5101 atattgctct ttgatctctt ggatcgtcgc caaacgggcg tctccttctt ggccataact
     5161 caatgaatca aattttcgag tcagcttttt aaagacgaac caggcaaaaa agaaagccaa
     5221 aggcagcgtg ataagcagct gcttgttcgt tcctacatga ataaacaagg aacgggaaaa
     5281 atagggaaac gtctgaaaca gctttccagc cgttgaaaag tcttggttta tcgaaagtgt
     5341 aaaggctttg ccaagaatcg gcagaaatga aacgataaaa ttgatcagat agttgctcgt
     5401 aaaataaacc aagaagaagg cactgaaaaa gccaagaaat gttattggtg catgacgctt
     5461 atgttcttcg gctagtcctt ctccttttat tgaataaccc cttccgataa aaagggcaat
     5521 ttttttcaga ccgaacacag aaaataatcg gtagaaaaag gagcgtttct tacttcgtac
     5581 tcgcatagac gctcacctta ttccaatacc gattttgctt ctttcggctt gtcctgtaac
     5641 aagtaagcag aagcgatctg ttgtttttga cccatggtta aatctttttt caacgcatct
     5701 gaaagctctt ggtagattga cgtaatggcc ttttgatctt gttgtagaat ggccagatcc
     5761 aataaaccat aatcagattc gcctagcttt gttaactgct ttaaggtgtc tgtgtcctct
     5821 ttttcaaaga cgacttgaac cacatggtca atattttcag gatattcttt agccgcttca
     5881 atatacttgc cagcactgat ttgatcgtca taagaaggaa ctgctggttc cgttgattcg
     5941 aaactcgcat aagaattctc tgtttgaaca gctgcagata cagttgtttg atttgaatct
     6001 tgttgggctt ttgcatgaac atagccgcca aatcctgcca cggcaagtaa aaccgcacca
     6061 cttaaagtga tttttttcca taaagacggt ttccctgcta cttgttgtgc agcacttgat
     6121 tgtgttggcg ctgcctgtaa aggtgtgcct tgttgttgct gttggataaa tgccatcagc
     6181 tgttgttgcg tggctatgtt ttcagcccct cttcgggtat gatcatcaat cagttttgca
     6241 agtatttgcg agatttccat ttctttttgt tgattttgtt gcttcgtcga ttcaacattc
     6301 tgcacggctt gatcttttag ggacaattct ttttctttta tagccaattc acgatcacgc
     6361 tgcgcaagta cggctttttc ccgtaactct tgttcatgaa tccgacgttg ctcttcaaag
     6421 acaggctgtg attcaatcag ttttgatttc attgattgta agtactgcag ttcttgatcc
     6481 gtgacttgtt gcactgattc gaccaggatc gtgcttaact cttcgagttg actgttctta
     6541 cgctcaatca atcggctgta ctgtgactta tgagcttttt cttcataaga agcagtgatt
     6601 ttttcttctt cttgttcgat tgcttcttgt ctcatttgat cgaattgtgc cgctttttcc
     6661 tgtttgtctg cctgcagttt gacatcgatt tgataaagct cgtcttgatg gcgcttattc
     6721 aatcgttcca gttcttcttg ttggcgcttt tggatcctcg ctttatcaag cgctaattga
     6781 ttctctaaag aagtgaacgc tgcttcgatc ttatcagtaa attcttcact tagaattgct
     6841 tttttagcag ctaattccgc aagacattct tcagaaccgt cgtctgcagt aatttcttga
     6901 taagcgatcg tcaacttttt gatcaatttg ttctctacgt cattttttaa tttatatagg
     6961 cttgagatca actgtttgta gaacgttgga ttgtattgcg tccggataaa ttctttcttt
     7021 cgctcactaa taaagtcatt tttatcggct aaatcaaaat acacgatcaa ttcttgcgtc
     7081 tggcgatcca gctttgcttc tagtttagcg atcagcgatt cgataggatt aaaaatcggt
     7141 tgagtcgtta aatccaaata ggcgtccaac ggtggaataa cgacacttgg aatcgattct
     7201 ttgactgtag aaggaacaat atcgtcctga gaagcttgtg ggctaagaat gtcttctttt
     7261 tccatttcta gatcttcaag aggaactcca tcttcttcct cgacggtttc gtaatcaggg
     7321 acatagttgt cttcgggcaa ctccaaggct tcttctacaa aattctctga aggtgattct
     7381 tcaattagtg gtacggctac ttgttgtttt ttcggcagta cacgagaaag cagattttcc
     7441 gcaacctccg gcaatttcac ttctggcaag gacaagccgc taagaaatga tggttttggt
     7501 tcgttgactt cttgttcaac aggttcaaat gactgatcga actcgactaa aagatcattt
     7561 tccaattctt caaattgttc tttttggctt ccctttaggt ttctaagctc ctgaataata
     7621 tcctcgtcga tcagtaggtc taaaggcaca tcttttgata aggggagagt gaactcccaa
     7681 tagtcttttg ttccttctat acgatggatc tctaaagtca tcttattatc cagcggccca
     7741 acatctgctt gaacctggta gaaaagctct ctcgcttcag gaagagaata cttctttttc
     7801 agggtcaatc gcttcgtagc tttgatttct tttggtaatt ttgtaaaacg caaaatataa
     7861 ttccccatgg ttctctcctt tataaaaaag atggaaagtt ttatttcttc ccatctttta
     7921 aacgtttgat attagaattt tttaccaatc ccttggaagt acgttgctaa tgaaacagct
     7981 gacatgatca cgatcatccc aactgctaca cgaaaccaga tgctttttgc gacgtctgct
     8041 tttcgatcgg aactcatgaa catgatgcca gctacgatcg tcgtaataac agccacggga
     8101 aacgatagtc tttgtaaagc tgaataaatc gttgttcctg ccttgtctaa ttcactgacc
     8161 caatcattcg ttggtagcgt tagtagtgct agttgtagtt tcattaacat gtttatttcc
     8221 tccatttttc acttggtttt tgtctgatac aagagtattg atttttgaaa gcacataaga
     8281 ctcgcattgc ttttcaatcg ttgattcttc ccattccttg tcttttcctt caagaatggc
     8341 cattttttta tctcgtaaat gatcattcgt ccattcctta aaggtaatac cttcttgaga
     8401 aagtaattct tttgcggctt ctacaaatgg tgacaccgct gattttttcc ctgccatatg
     8461 cttcttcctt cctggctaat tgattatcta aaaaaagaaa aacccctcga ttttttcgag
     8521 taggttcgct tttgtcttgg ttccatttcc ggcaatgctt ttcgttcttc gttgaattgc
     8581 cgattgattt cttggtaaag cttttctcct tccaagcggt ttgactcaag agtggatatc
     8641 tcctcttgca acatatctaa cagttgtacc gtgttccgtg taggatcaac gaccacgtct
     8701 tctaagatta tttcttgttc gccattgtca atcactactg acaatttcgt aggctccgtg
     8761 attgaacgct gtagctgttc catgatctgt tgtgcttgaa agataccgtg aaccatcttt
     8821 gtgttcaatg cacggtattt tcgtgtcaat tttttgttga cgtgcgaaaa atgtacccga
     8881 tattgcccgg acacacgatc acccctttca cattcttata ctttgctgcg tccaccactg
     8941 ttcccataca gcttttatga cgtcttggta gtcaatgact actttaagaa gaagcgcacc
     9001 cacgaccaaa ctgaaaatga gccgtaacaa tttggaaaat acttttccca tatcttcacc
     9061 tcacttgaga aggcgcttgt ccttcaattc ctatcttcac agagatattg tctggtagaa
     9121 ataaaactgg ataggtcgct ttgatcgtat aagccaccgt atctccaaac gcagcctttt
     9181 tccctagtga aggactttcg accgtatacc agccgtgaaa ttgtttattt gagtaatctt
     9241 tgagctctga aactgcttca gctgtcagac caccttttcg ctcaatctga taattgactt
     9301 gttgccgata agtagtgatc gtttgaagct gatagccgaa aacgacgacg ctcaaaaagg
     9361 cgagtacagt caaacaataa aacaacatgc tgaaaacttc tccctgacta tcttctacat
     9421 ccataattta tgctcctttc tttagagata aacatagact tttccaagtt ggaaaggaat
     9481 gatcgcaata gaagcaccaa gaacaaaaac caaaatcgct cctactagct tcatgtatag
     9541 accggaaaac cgactaatct taaagtggat gatttgttca ataccagcca tcatttcttc
     9601 gctggtgatc ctagaaagct gatcgatcac ttgcaaattc gctgatcctt gtgccaaacg
     9661 tgccaaagta accataaata accggctgct gtctgttcca gacatttgtt tggcgaacat
     9721 cttaaacgga tcgaaactgc ttggatttcg actcatatct aacatgagcc gttgtaagat
     9781 cttcttatcg atttcatgtg gcatccttgg aacgattcgc tcaaaaacag agcgaagatt
     9841 gatcccccct cttgtttgtt ttaatacagg gacaagtagc cgtgaaaatt tcgaaaattg
     9901 aatttggcgt tcaaatcgaa attgctgata aacgattttt actgatttga tcgtgttaag
     9961 gtagaccaac acactgacca cgaaaccgat tggaattaga tacaaactca ataacaaccc
    10021 aagtgcaata aaaaaagaaa agcagatacc aaagtttcga gcattctttt ttgaccactt
    10081 ttcaatattt tctttggtat tattcatttc taagagatct tcataaacaa gatccggaag
    10141 caagcgagaa aagaatggtc gattctcact atgttctgta gttttgtttc gaataacgag
    10201 attcattttt tggttcgcac ccccaattcc gtgacagaat catcaaaata ctgtttgaaa
    10261 aagctattta agatcatact aagcgaagta agacaaatgg ctgaactgac ataaccgatt
    10321 ggatggtggg catagccgtc catgtattgc gcaaaatcac cgttcatgaa gaaagaaacc
    10381 ccatgaaaca gcaaaataat agcgcccatt aaaataagga gtgtctgcac accgcttaac
    10441 atttcattct tcttctttag aaacaaattg actttttcta acattttact atggtgttct
    10501 gttagatatt gcaacgaatc cgtattgttt ttgccttctt gaataatcgt gtcaagctgt
    10561 tccaaatact gaccaaaaat cgcatcatca tggtactttt cggataggtt gttcaataaa
    10621 taagagaccc tttccggatc agaacctaca ccaagagcgg catacaagct tcgaagatct
    10681 tcagaaagtt ctccttctgt ttcatcgatc acataggcca aagactgaag aacagttaaa
    10741 tcattgtcca tcgtcacttg tgtgagattc aaaagaaatt tgtttctggc caccaatgac
    10801 tgcatctgat acctccgatt cacgaccttt ggcaaaataa accgatagcc gtaaatggca
    10861 ccaatcactg tacacccgac aacgatccac caaatatacg tcagaatcaa tgaaaaaacg
    10921 cctaagacta cagctgggag tccgacaagc tgaatgatct ctttttgctt taatcgctgc
    10981 ccgtaagcac tgtttaaagc atgtaattct tcaagattca cacggttctt agcttccgtc
    11041 tgaagttgat tgattcgcaa actcagccac cgccttttga agaatctgtt caagctcttc
    11101 cgatttatta atcttttcat tggtcatttc aggaaaatca aaggataaga agcgttctgc
    11161 tagcttttcc ttgtactgat cagacaactg acccgtagta acgtagaact gaccattgtt
    11221 taatacttgt ttaaacaaag tagtggccgt atcttttact gaatactcta cgatttctga
    11281 caaatatcga atttttttga aaataagatt ttccgaatcg atctcgatat aagcagtttt
    11341 gatctgaaat ccaaagtcga agaaagtaag gatatcttca gtgaccgctt cttcattaat
    11401 tttatacccc atactagaca tggtgactaa ccttcgagga tgcgctcgac aattcgttga
    11461 gtgaatcgac gtcaatcctg aatggtttgt ccgtgcagct tggatcattt cataagcttc
    11521 agaaccacgt gtctctgagg gaacgatcca tttagggtaa ttccgtaaag cagcttgaac
    11581 cagatctgaa atcgtcacga tatttcctgt aacgagagaa aaaatatcat gatctggcaa
    11641 aagttccttg attcttgttt caggaacatc ctcaatcata aagatacggt cttttttggg
    11701 cacaaatgat aacatcattt tgatcaattc cgttttccca gaaccagtct ccccaccgat
    11761 cacgctcatt gatccagcct tcatgattat ttttagaaat tccaaaacaa aagatggcgc
    11821 aaaatcgaca aacgtttctt cagtcaacgc tagacctggt cgacttaccc gtaaggacat
    11881 cgttgttcca gttggactga tcacttgatg catagcattt aatcgtaaat ttcctgatac
    11941 actatccaag ataggatcgt ttcgagtaaa tggctttcct acagcatccg cgaacttctt
    12001 tacgattcga aataccgtct tttcaggatc ggtcaactgt aattgttctg ccgtataaat
    12061 tcgtagttga tccgcacttt cgacggttag aaacgtacta ttccatccga tatccgtgat
    12121 tgactcgtca tgcaaaagta aacgatcgat aaattcaaaa ccgacgatct cattgacgac
    12181 ataggccgct tttttttgtc cttcatcatc ttttccaaat cgctgctctt cttgaactaa
    12241 tttagtgagc ttttcacgag aagctttaga cgtgaaggca cgcatcactt catccggata
    12301 gtgctcaact aaatcgtctc gaatcacttc gactcgacta tcttcaacta atttttccaa
    12361 ataatcgtct cgctcttctt cagtaactga cggtatatgt cggaattgat tggtagtcac
    12421 gatattcctt acatttctta acacgtcttt ttcatcttta aaaatggact ttttttctct
    12481 aattacaacc attgatttac cctccttccg gcgtacttaa aatgcatgtg gcttcgtcaa
    12541 tggatccatc ggactctaca taggcacgaa tgcctttgat tcctttgtta tctgcttctt
    12601 ttaagtagtt gtacgtcact tttactgttt tctttgcata gaatggctgc ttcttgatcg
    12661 tctgttcgaa ctctttttca aagctggctt gatccaaata aaaagcgcca ggctgaatgc
    12721 gtgcagaatc atctttgtta ctgattgcag ctgttcttaa cgcttcagta atgttaaatg
    12781 cttcggtatc tttcttggca tttcctataa aaaaccacgc aattagaatc ataaaaagtg
    12841 cccccacgat cataaccgtg atctgtcgcc aataatgtgt cattcctccc tctcctaacc
    12901 aagaaaaagg agaagacaaa gagtgtcttc cccgattatc tttttaacca ttattttgtt
    12961 ggcaatgttg aaacgtaaga agtgatttta gctagcattt gtgttgtgaa atcagggaaa
    13021 aacttgaaaa caacaccgcc gacgattgca actaaaccta ctgcaatcgc aacgttcatc
    13081 aatgtgtgag aatgattttg aactgtatcc atgaatgtca cctcccttca gtagaaatat
    13141 aaaccggcta ttaaagccga tcgttatttg aaaaacattt gtttgatttc atcaaaaaat
    13201 ttggtgcaga ctgccaagat cccagtaacg aataccataa cggcctgaac aaattgattt
    13261 tgttgaatcg ctgaggtcag cgaaccggaa acattgctgc ttccagaacc aaagtttaag
    13321 aacgaagaac tgatctgatt caagtaattg aacgggatcc cgatcaactg cttaaagaga
    13381 aagaaaaaga ccaggatccc taaactgcta aaaactactt ttcgcaatag gtgcctggac
    13441 tgttttaatt ccgatcgtac atcaaaaacg gctgaaccat acatattgcc caactcctta
    13501 ctttcctctt aaccacgtga tctctgcttt tgaccatcct tttggcacag tcgtatcagg
    13561 ataaaccggc gtgagtaaca attcatcttc tttctttgtt ttactatcaa tcgtcgcata
    13621 catgtttgcg tagaccgaaa gcgtttggtt catcgatact tgaataaagt tacggccaat
    13681 cacattcgat tgcagcgtca gattgtagtc ccctttttct gcccaaatcg gcaaataaaa
    13741 ctttcttccc ccatcaagta atttgtttgg tatttttttt gttggatttt ttacttgttc
    13801 ttgcgtatac aatgcacccg tcttctcctc tacgcttatt tgaggaaact caaattttgt
    13861 agaagtaatt gcggcagtcg tttgtttcgc tacttgattg accgtacgat ctaacgggat
    13921 ttgaatgttc ccttttgatt cgacatactg aagatctaaa taggtatcga ctagcttcgt
    13981 tggagaagaa agcgtaaacg agagtaatgg cgtatcttgg atctcactgc tgtagatcgg
    14041 ttctaatgtg attggtagtc catagcccgt ttttgacgaa cgctgttctt caaaatcaaa
    14101 ctgaagggtc tctttttttt ctacatacgc tttggttttt ctcgttgctt ctgtttgact
    14161 cactgcgcca taggaaagct tatcgttttt cagatccgca ttggtgagga ctttttctga
    14221 agctgtatag ccgaatgttg aaaaatcaat cgtatctgtt tcagccttta ctttgtcagg
    14281 atttttcagt acctgaaggg aaagagaata cggaaccttc tcacccttag taaagcctgc
    14341 agtaggcaaa tcaacaacta ggcttgaagc ataagaacca agtgaaatat cttttgtaaa
    14401 gacagttttc ttctgctttt gatcagttac agtcaatcga taaaggacat ttttcaattc
    14461 ttcttcgtaa ctcgctgact tagaaaaagt gatcgtcgcc ttcaagctgt ccgtcaatga
    14521 cgctttgcca gtcaatattg aaactttctc ggcattgatc tctaaagacg gtagataata
    14581 cgggatctca agtacaagat ctttgccagc aacgacccca cttctagtca ctttcgagcg
    14641 gtagcgattg cctttcacgt cgaatagatc atccagcggc ttatactcgt acgactcccc
    14701 atcgtacttg gtctcaacga cttgtttaag cagcgctttt tctgtttctg tcacatgatt
    14761 cactgtcagc ttgaatgctg gcaaagtagt tgtaacagcg tttgaagaag cagttttcgt
    14821 tcctgttttc acactacctt gcttactgaa ggacagtgtt gctttgtttt tagcagtcag
    14881 aacttcttgc tttagcatca ttttcgtacg tagatcatat gattttccat aaaatccaga
    14941 agttgtaagt gcagtacttt tcgcagtagc aatcactttc ccgttcgtca gatctttggt
    15001 aattgtaaat tgatctgtca catcactacc ttgcgccaaa acttgtaccg tagaaatagc
    15061 aaccgtttca gctggaaact ggtcttctat aacaaaatca gctaagtaac gagaccctag
    15121 tttttctacc gtacttgtcg ttaaatcata ttgtagaaaa tcatcggaag cagctgacca
    15181 agtgttgctg gttttgcctg tctcattctt gtctgatacc gtctttgaag cactcaattt
    15241 gtactgattg gtgattgtta atttcaccgg ctttgcttca gtcacatctg cagtaatggt
    15301 aaacgagtag tttttactat ctgtttcaaa accaaccaat gacgcttttt ccttaaaata
    15361 ataggatccc agcgatagat cctttacttt cagttggcca tttgtatcgg tttttaggtt
    15421 atcttgtaaa atggatccat ccgatttgta tagtgtaaat accacaccat ctaacgactt
    15481 ttgatcgacg tctgcgattt tggtaagctc tacagcccct gcgagtcgct cgtttgtcca
    15541 ggtcttcgaa acattcgtat tttcccctac cgtgattgaa aactccgttc cgtttagctt
    15601 gtagcctgaa ggagcggtta tttctttcac tttgactact gacccagcct ttaatcgttc
    15661 tcctagcttt gccttcccat ccgttcctga ttttacgtct gttgtctttc catcatacgt
    15721 aaagcggaag gtggcatttg ctagcggctt tttcgtatct gcatctactt tcaagacatc
    15781 cactgtccct tttcgcttca cattcacggt tacggaagtt gatacataaa aagacccagt
    15841 gaccagtttt tgatacgaac cgttcgccca aacagtagtc ggatattttt catttgtttt
    15901 cgaccctttc aacatattta cagtgatttt tgtcgcagta gatccatttg tatcaacagt
    15961 taatttattg ccactgatcg tcgcttttgc gccaccactg acggattcca catagaggtt
    16021 atgatcgccc caactgtttt tcacagtaaa ggtcgctttt tctccttcat aaactgtttt
    16081 agctgttgca tcccattgcg gacgtgtgcg gatctgacca gctcgtgtag tcattttgct
    16141 tttccagtcc gtcacaccag ctttgtgatt tggggtaatg gtgatacttt tgggtttttc
    16201 atttgaattt tcccaaacaa gaatttgtgc ggccatccag cgttgctttc cagcatcgtt
    16261 atttgcccaa ccgcctaatt cacggtaagc actaataatg gacgtgatcg tttctttttg
    16321 agaagtactc aaatagctgc taccggcatc cgtgtacgtc ttagctgccg taacccctgc
    16381 ttgtaaacaa aacgcatctt ttccatcgat cgtccgccga atcccacggt cttctgtgta
    16441 agaataacca ttgctgcgaa cgatatacat cagccaagga taacctgaag gcggattgat
    16501 cgttgtttca gagactgttt ttgataccga agctaataag gcaactttgc tttctttcag
    16561 catagaagga acatcattca agttgttcgt ttgattaagg atctcctctg cataaaagaa
    16621 acgtgtctgc atcaaaactt gttcagactc ttccgtattt ttggtttttg ccaattcttc
    16681 agcaacttga tcttcttcaa taaatggaag atcttcacta ttgactgccg actgcagcga
    16741 ttcaatatca tttagatcga tttcatcaaa gtctttgatt tcttgatctg cctgatccgc
    16801 tttttcctga cgcttttcat cgatccctac ttcaacagct gtaaagtctc cttcagtttt
    16861 ttcagcgatc tgcggcgtat ctgaagaaat cagctgttct ttgtctgatt tgtctgctac
    16921 cacgtacagt aagccatcag caccttgaac aacggttgat tcctgttctt tgtttaaggt
    16981 ctcttccgat actgcagcca cttcctgatc cgttttagcg gtatttgttt gttcttgtgc
    17041 caaaacataa ggaaagttgg ccatagcaag aagtgaaatg atcatgactg aaaacgaaac
    17101 gatgaatttg ggtttctttt tcattaacaa ctgttcacat cctctccttc aaaatggatt
    17161 ttcgaaaaag acggaggcgc tcgattacgc attctttatt ccttggccat ataagatcat
    17221 aggcggtctc cttcgtttaa ataacttttt gagttcttta tatttagcgt atgcagctct
    17281 tgttttgata tgtgttgttc ttgatcaacg ataaataaaa agtcgccatt cgtgaaaata
    17341 tcttttccag cggctttttg ttttgtttta tctttatcga taaccacgtc tacatgatcc
    17401 gtattaccgt gtaatacttg tttcccagcg accacttgca caacggtctt ataattactt
    17461 ttcgtgatca gtgaaacttc gtcaatttca aaaatacttt ggcgatcgac caagccatta
    17521 ttaataattt ctgccagttt attcacgctc aagcgaatat actgtaatgc ttcaccgttt
    17581 cctgtaaaac ttagggaaac ctcctttcct acggttaaat acaaggtttt cttttcagca
    17641 tccatctgtt tctgccctcc cttcactcat tttttatcta tcatttatgg actgcgacag
    17701 acgtttttca tacgcttcct ccttcttgag tattaaaaaa agcttgagat tccattccca
    17761 agcttactct tcttattctg ttaaacagct aaaccaaatt agttgatttt ctgtctcgtt
    17821 tacttataat aaataaggac ataatagttt gtttggtctt tctagcggaa actaggggga
    17881 cttttttaat tccttcaaac aaggtcaaaa ccgattccaa tcatctaatg cacgttgatc
    17941 tggcgataat tcgactaatt tttcattgta actgtcaaca agaattttgt gtttcaaggc
    18001 cttcaaacta tcgattttgc ttgataattc tgcccttatt attagcctat cctcgttcag
    18061 atccgtcaac tgcgcagtga ttcgacgaat cattctttca atttcctgta gagtgtcttt
    18121 cttgaaagaa tgatcatagt aacaattttt tagtaagtac tcttgatttt cttttgatag
    18181 gtattggtca atatcttcca aattggacat tttcccatat agatatagcc catacagaca
    18241 tatagtaaag ggtactatca tattgacaat actactcata ttgacctcct tttattgatt
    18301 taacaatatt ggtggttaac caacttattt tattgacgat tatctagtcc aatttatatt
    18361 tcttaaaaca atcatattaa tatacctata aataatacta atcagaaaag gtataccaat
    18421 atgaaaatga cacttgtacg tagagagcaa tttactgcaa ttggttacga tcctatttta
    18481 aaagagttac gaattgaatt taaaatcggc atatacacat atactgaaat tcctgaacat
    18541 atttatgatg cttttattca tgctcattca aagacattct tttataacag acatattaaa
    18601 aattatccct gtcgtaaagg tttttaatat gcttactagt tggtaaaatc tgtcacttgc
    18661 taacttcaat agactaaatc agttaaaatc ggtttatcat tttcgccaaa ctcaatcgta
    18721 gctacttgca ttgcgccata atctctacaa tattggacaa attcttttct taccttttga
    18781 aaagctgaat tagtttccca gcctaccttt gaaccatctg tattgataat gaaaaactct
    18841 tccccatttg ataaagagga aattattggg ctgacaaatt caccatctgt tttcatagtt
    18901 tgaactctct catgcattag cgggtcaaac atttcgattg ctttatttct caattcttgt
    18961 agtctttcat cataatcata tactaacgtt acatgattca atatcattcc catccttact
    19021 acatcctttc catcatttct tccactaatc aactatcttg gtggatagtg gaatcattgt
    19081 ttgtacgttc caaccctttt ttgcgcatat tcactgaatt tttctaatgt agtgccttct
    19141 tcttcacaga ctttagcaag tttcttagca tcataaccag tatcaactgc caagcctttt
    19201 tcaaagcttc tatacctagt taatgtttct tcttttcttt cccaaactct ttcttctgcg
    19261 ttcatcatca attcctccta gttgtttata tctgtcaact acaaactcaa gctttttcta
    19321 aaccgtcaga gtatttaact agtgtttcaa aaatacctaa acgagtagtt tctaaagttc
    19381 gtttccctga ctttaaatca gcaatcgtag tcctagatat tcctgtgtcc ttaactatct
    19441 ccttgatagc cgttacttcc aggtagtgat tcactttttc tttctgtact tcaagagagt
    19501 tcagtttctt ctcaagttca taaaacgcag aacttgcttc cgctaattcc ctgaatgaat
    19561 tatcgtaact ttcagaactt ttttctaaaa aatgttgaat ttcttcttcg ttcactgttt
    19621 ccggataata actaactggg ttttctattg ttacatttgt ctttcccaaa aagaaggttt
    19681 ctttgttcat cattctgcca ctcctcttat ttaaagtctg tcaattaatg acttcccctt
    19741 ccttccaaga aaaggatcct cttcgaacag tagcgttgtc tagcttttgc tgctcttctt
    19801 gttttaatct aatctgcttg tcacgccatg tctctttaaa tacacttgga ttagtatttc
    19861 tcattctttt cagttgattc gtcttcagaa tatttccctc ctgcttcagt tttacatttg
    19921 atatagacaa gaaataattt caaattttcc gcttttctag gagaatcttt atctgcaaaa
    19981 cactaattaa aagccaaatg attgcagtta ttgcaataag ccacttgatt aaaacaaacg
    20041 agataaccaa cctttgagtg ccgaataaaa ataatgtacc caagattcca ataccaaata
    20101 aagttgttat tacaatcagc gtcgacaata atcggctttt caacttatcc atttttctta
    20161 ttttattcgt gatcttgatt aattcagaga ctttcacttt ttatcacctt ttagaaaata
    20221 atgttgaagt agcgttgatc gtagcgaacg tgaaagtgaa aatgaagata aatacagcaa
    20281 ccttcagcaa tcccatacta actttaaaca tcatcttcaa gaaaaacttt ccaactgcca
    20341 gtaacttgtt tgtcttcgtc ataacttcca ctcccttttt aaaatttaat attaaccaat
    20401 taaacatctt ttgtttacca gattcgaaaa tagaacaaaa taaaagtcgc agatttttac
    20461 aaccggagaa atctaactcc tatgaaggcc ggatattcgc tcgcacacgt gcggtcttgg
    20521 caaatccttc aatgtttttg gctcaccagt tcattgtatt cctggttact ctaaacccct
    20581 tagtagccat gctgtttagt ttagaggggc gttctaatac ccacattcat ttattcaatt
    20641 agttctcaat actcgttcgt tatcaactca tatcaagaga ttttgctaga aactagcaaa
    20701 caaaagatgt ttaattaaat gttcaaaaag cagttaagct ctttttcgtt cctggtacca
    20761 ataattttga atcatttcct cgtattcctt ttcttctatc gcagtgatca aggcatacac
    20821 ttctaacgct cgttccgctc gatggttccg gactttgaaa tatttgtttt cccgtgtcgt
    20881 tatcagacga ccatttgaat ttaatcgttg agcaatgact ctttcctttt gatcaaaaag
    20941 aagtttgatt tggggaaaga tcgtttccgt tttggcagtc ccgaaaaata ttttttttgg
    21001 cttccctttt tcaaaaagag tcgtataacg ataggcgttt tccccttcga tcatccaatg
    21061 ctgcgtatta aatagttgta ctgatgtttt gattcttgct aaatctttaa cgtatactgc
    21121 taaccctttt gtcattgtta ttcctcattt cctagatcat atttgcataa aaacaaacaa
    21181 aaaggaggta aattcacaag aacgtgaatt tacctcctaa gagtcatagt ttctcaacga
    21241 agataaaaga tagtgttaac gattaatgag attgggacac attaatcatt aacacattgc
    21301 gaaagaacct gaaaaataat aatatatttt tcaatgatac actgactcaa tagtttcttt
    21361 ccacaatcat tatctgttat aattacatcg ttgtagaaaa cctacgacgc aaaggagctc
    21421 aatccgttca ggtgtggcat cacttgattg gaaaccaaag tatatcaatg acttctaaaa
    21481 gagcgtcaac tcttctagaa cgagatatct ttgggggctc ctttttgttt ggcttctta
//
LOCUS       DASNNU010000045        21143 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.045, whole genome shotgun sequence.
ACCESSION   DASNNU010000045 DASNNU010000000
VERSION     DASNNU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.045"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..632
                     /locus_tag="U8116_001580"
     CDS             <1..632
                     /locus_tag="U8116_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN8434580.1"
                     /translation="PLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQA
                     KIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTN
                     QAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTR
                     ISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1335..1550)
                     /locus_tag="U8116_001581"
     CDS             complement(1335..1550)
                     /locus_tag="U8116_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEN8434581.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1569..1805)
                     /locus_tag="U8116_001582"
     CDS             complement(1569..1805)
                     /locus_tag="U8116_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEN8434582.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2063..4216
                     /locus_tag="U8116_001583"
     CDS             2063..4216
                     /locus_tag="U8116_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEN8434583.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4185..5600
                     /locus_tag="U8116_001584"
     CDS             4185..5600
                     /locus_tag="U8116_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEN8434584.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            5633..6667
                     /gene="sppA"
                     /locus_tag="U8116_001585"
     CDS             5633..6667
                     /gene="sppA"
                     /locus_tag="U8116_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN8434585.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            6669..7190
                     /locus_tag="U8116_001586"
     CDS             6669..7190
                     /locus_tag="U8116_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN8434586.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7187..7573
                     /locus_tag="U8116_001587"
     CDS             7187..7573
                     /locus_tag="U8116_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN8434587.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(8069..8141)
                     /locus_tag="U8116_001588"
     tRNA            complement(8069..8141)
                     /locus_tag="U8116_001588"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8106..8108),aa:Lys,seq:ctt)
     gene            8522..9091
                     /locus_tag="U8116_001589"
     CDS             8522..9091
                     /locus_tag="U8116_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEN8434588.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            9078..9938
                     /locus_tag="U8116_001590"
     CDS             9078..9938
                     /locus_tag="U8116_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN8434589.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            10128..10832
                     /locus_tag="U8116_001591"
     CDS             10128..10832
                     /locus_tag="U8116_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN8434590.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            10837..12672
                     /gene="walK"
                     /locus_tag="U8116_001592"
     CDS             10837..12672
                     /gene="walK"
                     /locus_tag="U8116_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEN8434591.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            12669..13985
                     /locus_tag="U8116_001593"
     CDS             12669..13985
                     /locus_tag="U8116_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434592.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            13986..14855
                     /locus_tag="U8116_001594"
     CDS             13986..14855
                     /locus_tag="U8116_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEN8434593.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            14910..15719
                     /locus_tag="U8116_001595"
     CDS             14910..15719
                     /locus_tag="U8116_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN8434594.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(15822..16451)
                     /locus_tag="U8116_001596"
     CDS             complement(15822..16451)
                     /locus_tag="U8116_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEN8434595.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            16621..17913
                     /locus_tag="U8116_001597"
     CDS             16621..17913
                     /locus_tag="U8116_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN8434596.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(17989..18651)
                     /locus_tag="U8116_001598"
     CDS             complement(17989..18651)
                     /locus_tag="U8116_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN8434597.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            18876..19160
                     /gene="groES"
                     /locus_tag="U8116_001599"
     CDS             18876..19160
                     /gene="groES"
                     /locus_tag="U8116_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEN8434598.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            19211..20836
                     /gene="groL"
                     /locus_tag="U8116_001600"
     CDS             19211..20836
                     /gene="groL"
                     /locus_tag="U8116_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEN8434599.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         7196 a         3429 c         4263 g         6255 t
ORIGIN      
        1 gtccgttaga agttaaaaaa tacctcaaac aattccgacc aaagcaagga atatatttct
       61 tagatattga tttaaatcat gaagttaacg gtatagaatt agcagaagta atcagaaaat
      121 atgatgttca agcaaaaatc atttttacaa ctactcatga tgagatgtta cccgtaacaa
      181 taaaaagaag agttgaaacg ttaggatttg taacaaaaga tcaaacacta gatgagtatc
      241 gaaacgagat tgttgagtta ttgttattag cgcaagaaag gatagatgca acaaaacagg
      301 ttacgaatca agcatttgtt ttttccatag gttctcaaac atttactttt gatataaatg
      361 acatttattt tgtagaagct tcaaaattac cacatagatt atcattgtgt actaaagatg
      421 ggcagtacga attttatgga agaattagtg agcttgaaaa gaaatatcct atgttaacaa
      481 gaattagtcg agcgtgttta gtaaatatct ttaacgttaa agaaatagat tttaaaaaac
      541 gttcactata ttttgattca gagttggcca gaaactttac tttagggaaa gcacaaaaaa
      601 ttaaggagaa gttgaaagaa agcactgttt agtggttttt tctttaggga gattgcattt
      661 ttctaataaa atagattcct tcactagtgc aaatttttct gaatcctatt aaagttgtac
      721 caatttttat agctgttatt agttacgtgc ataacagcaa tattagttac tggaggaata
      781 tttctatggt ctggaaccaa aaaagggcca aatgaatagt tcttttcttg tttttattaa
      841 gtaccataaa ggggaattaa aattaaatgt atggtggaag atgcgaaaga tttggcaaat
      901 tctactaatt tttagaaagt gtttttcatt ttttagttaa aaaatttgtc ctgtcaagtg
      961 caatcctatg attgtacttg ataggatttg agataattac tttcctgttt ttataaattt
     1021 aacttgcagg aacaggcttt aatgcacatc cgacaagtac attaccaata cctcctgtaa
     1081 cggccgaaat aggtcctcct gcagcaccaa ctacagcgct gcaagtcctt ttgtaaaaat
     1141 atctctacca ttacatttcc aaagaatttt tatctgactt taataaaaga taaaaattta
     1201 atataattat aaaaactagc acaagaagct ttagtaaaaa gttctgtcca ttccaaactt
     1261 ttgaaaagat aaagaacata cctatgttcg taatcgacac aaaccaatct tgaggtttga
     1321 tattttttaa ttgtttacgc accggtccac atgctgaatc cagcacctaa taaagctcca
     1381 cctacaccac aagcagctat tgcataaggt cctccggctg aacaaagttt tactcctgct
     1441 gttgccccag aaaaagtagt gcctaaaact cccacggcac tcccaccttg aatatttttc
     1501 aactcttctg aagataatat aaatgttgtt tttgtgtttt taatatgcat actacttatt
     1561 ctcctttttt atttttttcg agcatcccag ccagctgcag cacctccagc gacagctcca
     1621 tataaccctc caacaacacc gcaagctggt ccgcaaattg ctgttcctaa acctgcacca
     1681 cctactgccc ctgtggtaag tccacctaca acttgcccca attttcccca agcattattg
     1741 cctccaccaa cagtttgtgc atttccacaa tacttagttc ctttacattt tgcatgttta
     1801 aacacgcttc tttaaataat attttttacg gtgctactgt aacatacgta tttgattaac
     1861 agtaaaaatc ttcctgaaac ttatcaagtt tattcttgaa tagtaaaatt tccaaaataa
     1921 atttgattca ggaatgaaaa agttagtgtt caagaactgt tttcaaagtt tttgtggaat
     1981 tatgagacaa tattttcact gtattaaagc tatattgaga atttttctat aaaaattttt
     2041 tagaaggagt tgtgttagat aagtgataaa atcatttcct tttgtgcagc aacaagatga
     2101 aaaagattgt ggtgttgctt gcctttcgat gattttaaaa tattttaaaa ctgatatccc
     2161 gattcataaa ttacgggacc tatctgggac tgatttagag ggtacttcag catttggatt
     2221 gaaaaagaca tttgaaaaat tgtcttttga ttgtatggct attcaagcag atagtgagat
     2281 ctggaaagaa agagatctta tcttaccatt aattgcacat atattggttg ataacagcta
     2341 tatgcactac gttgtaattt ataaaacgaa gggaaacgta ctatggattg cagatcccgc
     2401 taatggaaaa aatagaaaaa cgattgaagc gtttcaaaaa gaatggacag gtattctttt
     2461 attacctatg cctagagaaa cttatattcc tattaaagaa aaagtagctg gattaagttc
     2521 ttttttgcct ataatttgga aacaaaaagg attagtattc cacatcgttt tagcatcgat
     2581 tttcattacg ttttttggca taggaagctc atattatttt caaggtattc ttgatttttt
     2641 catccctaat caagcacgtt caactttaaa tataatttcc attggcttga tcgctgtcta
     2701 tttatttcgg gtattgtttg aatatagtcg tagttatctt cttattattt taggacagcg
     2761 aatgagtatg gcaattatgc ttcagtattt caaacatgtg ttaactttac caatgaattt
     2821 ttttgctacg agaaaatcag gggaaattat ttctcgtttt cttgatgcca gtaagattat
     2881 tgatgcttta gcaagtgcta cgttatcaat atttttagat attggtatgg tgttgcttgt
     2941 tggggttacg ttagctattc agaatagtac gttgttttta atcactctat gctctttgcc
     3001 tttttatgta gttgcaatat tcgctttcgt gaaaagctat gaaaaatcga acagggaaga
     3061 aatggcagcc ggtgcagctg taaattctag tatcatagaa agtcttaaag gaatagaaac
     3121 aattaaagca tatagcggtg aagagaaagt atataaccgt atagaccatg aatttgttaa
     3181 attaatgaaa aaatcatttc gtacggttac gcttgataat attcaacaag gaattaaaca
     3241 tttgattcaa ttaattagca gtgcattaat tttatggcta ggttcttatt ttgttatgga
     3301 taatagaatt agtctaggtc aacttatcac atacaatgct ttactagtat ttttgacaga
     3361 tcctttacaa aacattatta atttacaagt gaaaatgcaa acggctcaag ttgcaaataa
     3421 tcgtttaaat gaaattttcg cgattgattc agaaaaatcg aaccagtctt ttgaatatat
     3481 gctttctgaa caattatttc aacaagatat cactgtaaaa aacgtttcat tctcgtatag
     3541 catgaaggcc ccaacgttga acgaaatttc atgtacaatt ccttcgcaca gtaaaacggc
     3601 tatagttgga gttagtggtt ctggaaaatc aactcttgca aagttatttg ttcgttttta
     3661 tgaagcatca gaaggatcga tccattatgg aaacattaat cacttagaca ttccttttca
     3721 gatattacgc gatcgagtga catatttgcc tcaagagtca tttttcttta gtggaacaat
     3781 cattgagaat cttatattcg gtttagatag cccaccaagt ttcgaacaaa taatagaagt
     3841 ttgtgagata gtccaactaa atgaatttat taatcaacag ccattacgtt acgagacatt
     3901 gttggaagaa ggtggaaata atctttcagg agggcaacga caacgattag ccttagcacg
     3961 ggctttatta aaaaatccgg acatattaat tttagatgaa gcaactagtg gattagatac
     4021 cttgttagaa catgcaatta tgaattatct cctcaattta aaagaaaaaa cgattatttt
     4081 tattgcgcat catttaccaa tagcaaaagc atgtgatcaa attttagttc tccatgaagg
     4141 gcatttggta gaaaaaggaa cgcacgatga acttcgtttt gccgatggta tctatcaaaa
     4201 attatgggaa atctaggaga gggaaacaat ttatgcagaa taataattgg ttcgaacatt
     4261 ctagtatcta tactaaacaa catcatagct tctaccgttg ggtgttatac cctgtaattc
     4321 tttttttatg tattattgga ttgtttttaa cctttgctaa aaaagaggtt gttattcgga
     4381 caacggctca attgaacccc gaaaaaatag agaagttaca agtgccatta gaagctaaaa
     4441 taatggaaaa cttcctaaaa gaaaatcaat ttgttcataa ggacgatgtg ttggttagat
     4501 tggattgttc tttaatagag aatgaaaagg ctcagataga acaagaaaac caacgtatta
     4561 ctcaacaaat aaagatggct cagctattta ttgaaagtat aagtaaagga aaaaatttgt
     4621 tttcaacgga tgacagtttt ggctacagta atcaattaaa gagcatgttg tcagaaaaag
     4681 aatcactccg ctacgctttg aagcaaagtg aattaaatga tcaaaagcaa ttagaagtat
     4741 acgaaaagac aaaaagacaa ctagaaaaac aaattgagag ttcagatagt aaattacaag
     4801 aatggcaaca agtacaggta gcttggagta ataatcaatc attaaaagat ttttcaaaag
     4861 aaatgatggc aaactatgag aattggcaag aacaactaaa taatgtttct gatgatcaaa
     4921 aaaatcaagt gaaactgaca atttcagcaa gcataaatga acaaattgag caactaaaaa
     4981 aagaagtaga acagtatcag tcagaaaaag ctaaattagt taaaccaact acttctgaga
     5041 atgacagaat tagtcaaacg gaaaaaggaa agcaagagct agaacaagca ataacaactg
     5101 taaaacaaac agttacagag ctaaatgaaa aacaagagaa gaaccagtca gttatcaaaa
     5161 gtttggacga tcaactttca aaaggaatac taaaagctcc tgtatctgga acggttcatt
     5221 taaatgaaga aacaaaggga caagcagaac tagccaaagg aacagtgctt gcagaaattt
     5281 atccgatcca cgaaaaatca aaaatgaaat ttacagcact tttacctgcg aatgaaagta
     5341 cctatattaa agaagggatg aaggttcatt ttaaattgga tcaaaaaggt ggggctccga
     5401 ttacgatcga cggaacttta gatgaaattt cagaaaattc tactgccact gagaaaggtg
     5461 ttttttatgc agtgaaagga acgttaaaaa caactcaatc gtttccgttt cgttatggat
     5521 taactggaga gttgtcttta attgtaggag aaaaaacata ttggcaacaa attgtcgatg
     5581 tgtttctaaa taaaaaataa ttatttttac taagaatata ggaggagttt atatgaataa
     5641 aagaagatgg attgctgtgg gagtagcgtg cggattattg ttactatcca ttatcgtcgc
     5701 gctaattcca ggaaaagata aagaggaagc atcaaataca accctaacag gaataaataa
     5761 gattttttac ggatctaacg aaatcacaga agaaacgtta gaagaaggcg catcaaataa
     5821 aaagattgtc aaactatccg tcaatggagt tattgcagat acaggtgaaa gcaatctttt
     5881 tagtagggaa caatataacc atcagaattt tttaacacag ctaaagaaaa ttcaagaaga
     5941 caaagcggtt aagggagtgt tattagaagt aaattcacct gggggaggaa tatatgaaag
     6001 tgctgagatt gctaaagaaa tggcaaaaat taaaaagcta gatattccta tctatactgc
     6061 atttaaaaat acagcggcaa gtggagggta ctacatttct gctggttcag ataaaatatt
     6121 tgctacggaa gaaacaacaa ctggatcaat tggtgtcatt atttctggtt taaattattc
     6181 tggcttatta gaaaaacttg gcgtaactga tgcgacgtat aaaagtggtg cattaaaaga
     6241 tatgatgtca cctcaacaca aaccttcaga ggaagagaat aaagttatac aggaatttgt
     6301 gatgagtgct tatgatcgtt ttgtcaatgt tgtagcaaaa ggaagaaata tggatactaa
     6361 tgcagtgaaa gaacttgcgg atggaagaat ttatgatggt aaccaagcag tagaaaatgg
     6421 gttggtggat caaattggtt actctgaaga tgcattagat tccctaaaaa aagagaagaa
     6481 attaactgat gcgacggtta tagaatataa aaatgacaca actggctttg cttcttcttg
     6541 gttaggtaat aagattgctg aatggcaagg gctaaaggca actacatcag atcgtgtaat
     6601 ttcagttttt gaaaaacttg gattttctga tactccaaaa ccaatgtatt actatggagg
     6661 gctataaaat gaataagaag agtggcattg aaactttgcc taattatgta tttgcgggat
     6721 tttggttacg attttttgct tttcttattg atttaatttg tattagcatc atcacccatg
     6781 ctactatagg tctcttttac aaaatcatgg gactggaatc tgacaaatcc attattactt
     6841 tttatggtat agcgtcatta gctatttatt taacctattt tgtacttttg actaagctga
     6901 attcaggaca aacaattggg aaaatgattt ttgggataag agtagtgagc tttgaggaaa
     6961 aagagctaag ttggcaaact atctttattc gtgagtgtat atgtaggttt atattaaaaa
     7021 attttgtatt tcaaattggg tatttggtgg ctgctttcac gcccaagaaa caacatattg
     7081 gggattattt ttctaacacg tcagtggttg ttattcgttc attagaagct attaactatg
     7141 gtcaaaaaat ttctaattct aaaacactta atctggagag aattaaatga ataagagaaa
     7201 aacagagttt attttgattt taattagtgg aataacaggg attttatgta gtcttttagc
     7261 attgatgggt ggtatgtttc tatcttctat tgattcaact actttaaata ctacagaact
     7321 aaccagtaag gatatagata tgttagtcaa tctaagcggg aatgttgttt tatttggagg
     7381 aattacccta gttttttcga taatgatttt cataataggt tttttagtaa gaaaaaatca
     7441 aaaaacagtg gtctatggta ttagtttact cattttatct attattccat tttttctttt
     7501 aacattattg tgggtaattc ctggagtttt aggtattatt gcgggattga tgcttctaat
     7561 aagaaaatct taattaggca atgtgtagac aacgtgaaac gattaatcaa ttaaaagcta
     7621 aatacgtttt gagaaatgat aacttgtagt cgatggaaga catgtagata aaaaaactac
     7681 gtgtctttta tttattctaa ttttttcgaa aaaaagacac tctctgattg ttgtattaat
     7741 ggaaaagggg aaagcagcaa gtaaatttag tttttctatt aaatattctt cactttttaa
     7801 ccttttatag tacttagatt taatcctata tgtttagaga gttcattggc agcatgattt
     7861 tgcaaagctt ctcgaattat tgttatatta gaaactttac tgttcataat tatgcttctt
     7921 ttttaactca aagtccaaag ggctacagtg gactttgagt taaaaataaa aagccctagt
     7981 tattttgaaa aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat
     8041 ttacctgttc cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc
     8101 tgattaagag tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata
     8161 atagtagcac taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt
     8221 tacttcctta taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat
     8281 accaacaaac aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc
     8341 tttcccgccg tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag
     8401 ccttcttgtt ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta
     8461 gattgaatgt ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat
     8521 gatggaaaaa atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct
     8581 gcctcactgg gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt
     8641 tgatcgttat ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat
     8701 ggtaaacaat tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa
     8761 agaacacgta gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga
     8821 aatcaaagaa gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct
     8881 attttgcgat gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc
     8941 tcatccgcaa aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac
     9001 tttatcgttt gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt
     9061 aaaagaggag aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg
     9121 atgttccaga agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt
     9181 ataaagatca ggtatataca gataaaattg acattacccc tgaagagatc tatcaacgtt
     9241 tgccagagga aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg
     9301 agcaaatcaa aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga
     9361 gcgggacata taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg
     9421 tactagatac gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat
     9481 ggctggaaga aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa
     9541 gaacaaaagt tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg
     9601 gtttagttgc ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg
     9661 aagaagggat ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa
     9721 cggttgctta cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc
     9781 atggggatgc gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac
     9841 aagcaaacca aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg
     9901 gattattggg tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag
     9961 gtgaaaatca gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg
    10021 cactttcatt ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata
    10081 cggtataatg agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt
    10141 tagttgtcga tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag
    10201 gctatgacgt ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag
    10261 aacctgattt gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc
    10321 gtgaggtacg caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa
    10381 ttgataaagt cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca
    10441 accgagaatt agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg
    10501 aaccagaaga ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag
    10561 cttacatggt gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac
    10621 tcttttactt ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg
    10681 tttggggtta tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc
    10741 gtgaaaagat cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg
    10801 gttactattt gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt
    10861 cgatcagtca attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa
    10921 attattggtg cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa
    10981 gatatgaatc aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa
    11041 gataatgcag atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg
    11101 actagcgata tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc
    11161 gatgtcaatg aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc
    11221 acgactaaac gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa
    11281 ccgatcctgt ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc
    11341 gaacaaaaat attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt
    11401 gcggcattta tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc
    11461 ggtgaaatga gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa
    11521 gtgcatggac aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt
    11581 attgaagaag ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc
    11641 catatgacag acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat
    11701 atggctgttt ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca
    11761 cttttagata tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg
    11821 gtacttgacc gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt
    11881 tctatgatcc gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg
    11941 acagaacaag aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa
    12001 cttcgtacac cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg
    12061 aaagacgagg aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg
    12121 attcgaatga tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt
    12181 caattagaat atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg
    12241 atggtgggaa atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt
    12301 tgggtcgaag tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg
    12361 atcaagtatt caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac
    12421 gtgattttaa gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg
    12481 ttcgagcggt tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt
    12541 ggattagcta tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc
    12601 aaggaaggaa aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa
    12661 tggtgggaat gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt
    12721 taagcatata cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg
    12781 tagatgagac aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct
    12841 ttcttccgct tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa
    12901 atctcgtgta ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag
    12961 tagtttcagg agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt
    13021 tggcctataa tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag
    13081 atgtgtcgaa cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc
    13141 aaggaaataa aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata
    13201 tttcgttgaa ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag
    13261 aaatgacaac agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac
    13321 tgaagaagta tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt
    13381 tccagaaacc agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg
    13441 ggaatgaaac aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc
    13501 cagagaaaaa agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag
    13561 gcagttcttt agggaacctc agatattttg accgcagtga caatacgatc gattatcgga
    13621 tattcgtgga aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag
    13681 ttggcggtga aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc
    13741 aagtgccgat accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc
    13801 agttaaaagc taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat
    13861 ggcaaaatgt caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat
    13921 ataaagataa ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga
    13981 attaattgga tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg
    14041 tgtttttatt cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta
    14101 atcagactga atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga
    14161 tttcgtctga gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt
    14221 cattagcgaa ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag
    14281 acggtgatgt tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc
    14341 cagataaaat tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg
    14401 gtaatgatta tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc
    14461 aagcaagcca agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt
    14521 ctttagaaaa aaacgataat gattatagca tcacgaaata catgcagacg catttatctg
    14581 atatcgagca gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat
    14641 tgtacatcaa taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt
    14701 cctgtattct gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg
    14761 aaacaagcga taaagtggtc caaattgaga gcgtcaacgc tttcagcaat acgatcgtga
    14821 caaacaatac tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt
    14881 tacaaagtaa gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca
    14941 gtatcctagc aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc
    15001 ttttggtcga tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc
    15061 ggacaccgga ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg
    15121 gcgtgggtgt cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg
    15181 aagcgatgga accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg
    15241 ggaaagtcag aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag
    15301 cggctccgca attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca
    15361 ccggatattg cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg
    15421 aaagtaatca tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc
    15481 gtattttagg agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg
    15541 tgatcggtaa tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga
    15601 aagaacttgc tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac
    15661 atgattttga aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa
    15721 cagttcaatc aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc
    15781 ttctattttt atttttttag catattaaaa aaataaccgt attatcgact gactacactg
    15841 atgtgctgct tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct
    15901 gccatgctga tcgtactgtc gctagcagcg tttctttgta aataatcatc acttaacaaa
    15961 taagtacctg tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa
    16021 tgtagatttt tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga
    16081 tagaagggtg catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag
    16141 aataacgaag aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca
    16201 atcaaatggg acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca
    16261 tcaacgacaa catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt
    16321 ataaacggaa catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa
    16381 tctctggaca atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt
    16441 gcaattttca taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat
    16501 tcgtcaaaga atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca
    16561 ttgggtatag ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa
    16621 atgaaagagt ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga
    16681 cccttaactg ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg
    16741 ctgtttttct tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca
    16801 ggattttcta tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg
    16861 atcgtttttg ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc
    16921 ggcataacag ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat
    16981 actgtctgga caggtgctga agggatacta ggagcagtta caactgggct catcatcgga
    17041 tggggctatg cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct
    17101 gaaaatgttt ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg
    17161 cttatcggtt cagcggtttt tcaacatttg ttcagaatga ctgttgttca aggagtatgg
    17221 acatatgtcc agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta
    17281 ggtttttttg ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg
    17341 ttggttagtc ccgtcttgca agcgaattca ttagcaaatg cagaaatctt agcctcagga
    17401 caagagttga cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc
    17461 atgacggtta cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg
    17521 aaatcgaaga aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc
    17581 aatgaatcaa ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt
    17641 attttcgcac cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt
    17701 cctttattta cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta
    17761 gtaggcggag tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt
    17821 tatttcccat ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa
    17881 aatgatggag cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag
    17941 attgggaaaa tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc
    18001 tttttggaca gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat
    18061 cgatgtccag atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa
    18121 gtagattagc agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc
    18181 tccgacccaa aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa
    18241 ctcttccatg atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat
    18301 catctggatg atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc
    18361 taacatgaca gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat
    18421 aacaatcttt ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg
    18481 cttaaaataa aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt
    18541 gatcaattca gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa
    18601 aattagacca tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct
    18661 ccttttaagg gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca
    18721 taatttcaat aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa
    18781 aaagagggag aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta
    18841 acagattaaa aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg
    18901 tcattatcga agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg
    18961 ctgctaaaga gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag
    19021 aaaacggtga aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat
    19081 acgccggtac agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata
    19141 tcatggcgat tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg
    19201 tgaatcaatc atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg
    19261 cggggtagat aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt
    19321 cgtactagaa aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa
    19381 agaaatcgaa ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc
    19441 atcaaaaaca aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc
    19501 aatcgttcgc gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg
    19561 cgggatcgaa ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt
    19621 tgattcaaaa gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg
    19681 acatttgatt gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga
    19741 atcaaaaggg atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta
    19801 cttatcacaa tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata
    19861 tatcttgatc acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca
    19921 aatcttgcaa caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt
    19981 gccaactctt gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc
    20041 tggtttcggc gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac
    20101 agtaattact gacgatctag gtttagaatt gaaagatgca acaatcgaaa acttaggaaa
    20161 tgcttctaaa gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa
    20221 agaagcaatc gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga
    20281 ttttgaccgc gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt
    20341 taaagttggt gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc
    20401 attgaatgct acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct
    20461 agtgaacgtt atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat
    20521 caagatcgtt gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta
    20581 tgaaggatca gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc
    20641 tgctactgga gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac
    20701 acgttctgcc ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt
    20761 cgttgcagat aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat
    20821 gggcggtatg atgtaagaaa aagcaacttg cttatctaca actgatcatt agtgaaagac
    20881 agataaaaag aggctggaac agaagcgttt aactccaaga aataagaaga aattcacgga
    20941 aattgctttt caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc
    21001 gctgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt ctcacgctct
    21061 tttttgtgtg taattataat gaaaatagtt atttttaata gttataatat tttatctatt
    21121 aaaagtaaat atccattcac taa
//
LOCUS       DASNNU010000046        21049 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.046, whole genome shotgun sequence.
ACCESSION   DASNNU010000046 DASNNU010000000
VERSION     DASNNU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.046"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..856)
                     /locus_tag="U8116_001601"
     CDS             complement(<1..856)
                     /locus_tag="U8116_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8434600.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSAD"
     gene            1113..4502
                     /locus_tag="U8116_001602"
     CDS             1113..4502
                     /locus_tag="U8116_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN8434601.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            4505..5926
                     /locus_tag="U8116_001603"
     CDS             4505..5926
                     /locus_tag="U8116_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN8434602.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            5923..7800
                     /locus_tag="U8116_001604"
     CDS             5923..7800
                     /locus_tag="U8116_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN8434603.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            7892..8734
                     /locus_tag="U8116_001605"
     CDS             7892..8734
                     /locus_tag="U8116_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN8434604.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(8886..9761)
                     /locus_tag="U8116_001606"
     CDS             complement(8886..9761)
                     /locus_tag="U8116_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN8434605.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            9968..10998
                     /locus_tag="U8116_001607"
                     /pseudo
     CDS             9968..10998
                     /locus_tag="U8116_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            11087..11392
                     /locus_tag="U8116_001608"
     CDS             11087..11392
                     /locus_tag="U8116_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEN8434606.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            11571..13586
                     /locus_tag="U8116_001609"
     CDS             11571..13586
                     /locus_tag="U8116_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN8434607.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            13588..13872
                     /locus_tag="U8116_001610"
     CDS             13588..13872
                     /locus_tag="U8116_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN8434608.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            13898..15271
                     /locus_tag="U8116_001611"
     CDS             13898..15271
                     /locus_tag="U8116_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN8434609.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15354..17369)
                     /locus_tag="U8116_001612"
     CDS             complement(15354..17369)
                     /locus_tag="U8116_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEN8434610.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17391..18605)
                     /locus_tag="U8116_001613"
     CDS             complement(17391..18605)
                     /locus_tag="U8116_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN8434611.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            18825..19769
                     /locus_tag="U8116_001614"
     CDS             18825..19769
                     /locus_tag="U8116_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8434612.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            20065..21009
                     /locus_tag="U8116_001615"
     CDS             20065..21009
                     /locus_tag="U8116_001615"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN8434613.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7153 a         3880 c         3973 g         6043 t
ORIGIN      
        1 aatcagcgga tgaaaacgaa cagtaggaca tattttttcg tatctgctca ctgatttcat
       61 ttttttcttt ctccgaaaat tctgaaaaaa tcgtattgac aaaatagttc actactttta
      121 tatagctgct gtacttcaat tttcctaaga gatttttcaa gattaagaag tcttttttat
      181 cgatatttat caactgtaca taagagtctc cagctttcca ttcaaatgga gttccttggg
      241 attctctcat gagatgcaca acaaaataca taataaagaa agattgctca tatgggcttt
      301 cttcctgaaa caaattcatc tgacaattat tcttgaccat aatttctttg atcttctttt
      361 tttcgattgg aaatggcaac gtgcgtcttc cgtagtaact taaaaagaaa aaggtaacaa
      421 agatacgcaa ctggagctca cttttaaggc tagttaactg aaaaccttgg tcaacgatca
      481 cttttaataa atagctttct aaaatttgat tgcattgttt caacttacgt gtaaaagaag
      541 accggctgat ggaaattttt tcacaataat actcaatcgt tttacccgga ttgacaataa
      601 tctcatttaa taaacgactg tttgtagaac tttgcgacat ttccatcaga acatttaaat
      661 aagctggtac attcaaatgt tcaagacgat accccaattt ttgtgaagat tgaatcgtta
      721 catccgttcc ccagcggatt ttgatctctt cgatatacct tacaaatgta gattctgaac
      781 taccaattcg tttggcaagt tcttttttag tcatccactt gtcagaaatg gacagaattc
      841 gaatgatttt ttccatcttt ctatagttta attccaacaa cctgatcata tgtttttctc
      901 cctcaaccct tttctttcaa tgaaacgctc attaatcgaa ggaatcaact tcataaatga
      961 aaccaattgg tatgaaaatt ggtgtgattc cctttagcgc ccttcgtata ataattatca
     1021 tattaaaata aataaaatta tgtaacgcct gtttatttta ataattttca ctattcacgt
     1081 caaaacagaa aaaaagaaaa aggaggggaa aaatgacgac cacagggaag aaactgaaag
     1141 ttattttcat gctgataata ttgagtttat caaactttgt gccattatct gcaatagcag
     1201 acactacaga tgatccaaca gttttagaaa caatttcagc tgaagtcatt tcggatcagt
     1261 ctggaaaaaa agcactgaac atcaagctaa atgcgaataa caccagtgct gaaaagatag
     1321 aaaaagaaat tggtctagtc gaaaattact taagtgatgt ggaaagaaaa gaaggagatg
     1381 gctatgctta tcaggtaaat agcgggaaaa ttacgttgga aatctcatca aacactaaac
     1441 aaactatcga tctgagtttt ccaatcgatc cagcacttta ccacagccag gcaaacaagc
     1501 tgatcgtcga taataaagaa tatgacatta ttgatgagac agaaaataag aaagatacag
     1561 atgtgtcagt accaaagcca gacgaaatag aagaagaatc atcaaaagaa aacgaaaatt
     1621 ctgtcagccc atttacattg cctacattat ccttgccagc tgtgagtgtg ccatctaatc
     1681 aaacgattcc tacagaatat acaacagatg atcagggcac ttatcctaaa gccagttggc
     1741 aacctacagg aaatacaaat gttcttgatc atcaaggcaa taaaaacgga acaaatcaat
     1801 gggatggtat aaattcttgg aatggagatc ctaatgatcg gacccattcg tatatcgaat
     1861 atggaggaac cggtaatcaa gcagactatg cgatacgaaa gtatgcaaag gaaacaagta
     1921 cacccggatt gtttgatgtt tatttgaatg ctcgtggaaa tgtacaaaaa gatatcacgc
     1981 ctcttgatct cgtattggtc gtagactggt caggaagtat gaacgacaat aatcggatcg
     2041 gtgaagtaaa gattggtgtc gatcgttttg tcgatacttt agcagatagc ggtatcacag
     2101 acaaaatcaa tatgggatat gtcggctact caagcgaagg atatagctac agtaacggtg
     2161 cagtacagat gggttcattt gattcagtga aaaatcaagt aaaatccatt acaccttcac
     2221 ggacaaatgg tggtactttt acacaaaaag cactaagaga tgcaggaagc atgctatccg
     2281 ttccaaatgg acataaaaaa gtgatcgttt tgctgacgga tggtgtacca acattttcct
     2341 ataaagtaca gcgggtacac gcacaatcaa gcagcaatta ttacggaact cagttttcta
     2401 atacgcaaga tcggccggga aatacttctc taatctcaag aatctatgat gcacctgacc
     2461 aaaacaatct atccagaaga atcgacagta cgtttatcgc aaccatcgga gaagcgatgg
     2521 cactcaaaga acgaggaatc gaaatacatg gtcttggcat ccaacttcaa agcgatccgg
     2581 cagctggtct ctcaaaagca gaagtagagt ctcgtatgcg acaaatggtt tcatcagatg
     2641 aaaaaggcga tctttactat gaatcagctg atcatgcaac agatatctct gaatacctag
     2701 ccaaaaaagc tgtacagatc tcagcaactg taagcaatgg acaaataaat gatccaatcg
     2761 cagaaccatt catttatcag cctggtacac tttcagtcaa gagtgtgggg acaagtccta
     2821 caacggtcac tccatctatt tccatagaag gaaataccat caagagcaat cagatctatt
     2881 taggaaaaga ccaagaaatc caaatccatt accaagtgag aatccaaaca gaaaatgagg
     2941 acttccatcc aaatttctgg tatcaaatga acggcaggac aactttccag ccaaacattg
     3001 ataccaatga attagctgaa ttcggtatac catctgctaa agctcccgga gtcagtcttc
     3061 acatcaaaaa gttatgggaa gaatttgaca acaatctagc tgatcgtcca gatcaagtta
     3121 cttttgagat tcaacgggaa catacgacaa atgctgcagc ttggaaaaac ggatatattc
     3181 gaatcattaa accagctaaa gatacaacaa atacgtggga acgtgcagac attgacaaat
     3241 tatctgcaaa tagcggagaa agttatcaag agatattatc actacctcaa tacaataatc
     3301 aaggtcaagc attcagttac caaacaatca aagaattacc tgtaccagga tacgattctc
     3361 aacaaataga tgcaatgaca tggaaaaata ctaaacaatt cacaccgtta aacttgaaaa
     3421 taacgaaaaa ttcctctaca ggtgaaaagg atcttattgg cgctgttttc aaattaacag
     3481 gagattctat tgatacttta ctaacagatc atggcgacgg aacctattct cttccagaaa
     3541 atgtcaaatt gcaaaaagaa atgacctata cgctgacaga aacaaaagct ccagaagggc
     3601 atggattaag caaaaagact acttgggaaa tcaagatcgc ttctgatggt acggtaacca
     3661 ttgatggaaa aacagtcact acttccgatg atacgatcca gttgactatt gaaaatcctt
     3721 ttgttgaagt tcctgtagca gtacgtaagt atgcgatgca agggacggac aaagagataa
     3781 atcttaaagg agcagcattt tccctacaga aaaaagaagc aaatggtact tatcagccaa
     3841 ttgacagcca aacaacgaat gaaaaaggtc ttgccagttt tgattcactc acacctggta
     3901 aatatcgagt cgttgaaaca gctggtcctg ccggatatga tacttcgccg ggaaattatg
     3961 aattccaaat cgataaatat ggaaaaatca tttacacggg aaaaaatacc gagatgacaa
     4021 ataatgtatg gacgctcact catcaaaatc gactaaaagc gtttgatcta acggtacaca
     4081 aaaaagaaga caacggacag acattaaaag gagcaaaatt cagactgcag ggaccagaaa
     4141 tggacttaga atcgccaaaa gatggacaag aaacagatac ctttctattc gaaaatttaa
     4201 aacctggaac ttatacgctg accgaaactt ttacaccaga aggataccaa ggtctaaaag
     4261 agccagttac tatagttata cacgaagatg ggtcaattca agtggatgga caagatcatg
     4321 aatctgttct gtcaccagga gccaaaaaca accagatttc tttagacatc acgaatcagg
     4381 caaaagtacc attacctgaa acgggaggaa ttggccgttt aggaatctat ctagtaggga
     4441 tgattggttg tgcgttttct atttggtatc tttttttgaa aaaagaaaga gggggcagct
     4501 aaatatgaaa aaacttggtt ggcttagtat gtgtctcttc ttgttactat ttaaaccagc
     4561 ttttactcag gtagcaacag aaacagaaac agaaatggtt cagattactt tacacaaatt
     4621 gcttttccca aacgggcaac tgccgaaaaa tcatccaaat gacggacaag aaaaagcttt
     4681 attacaaacg tatcgaggat taaatggtgt cacattccaa gtttatgatg tcacagattc
     4741 tttttaccat ctacgggaaa agggcaaaac ggtagaagaa gcacaagcag agatcgcaaa
     4801 aaacggtgcg tcttccggta tgtttaccgc agaagcaaca actacaactc ttaacaacga
     4861 agatggtatc gcttcttttt ctctggccgc taaagatcaa gaaaaaagag ataaagcgta
     4921 tcttttcatt gaatccaaag taccagaagt cgtcaaagaa aaggcagaga atatggtagt
     4981 tgttcttcct gtacatggac aaaacaatca aaaactttca actatccatt tgtatcctaa
     5041 aaatgaagaa aacgactacc ctgatccacc ttttgagaag gtattagaag agcctagaaa
     5101 tgattttacg attggtgaaa aaatcactta ttccttgcat acgacaattc ctgtaaatat
     5161 ccttgactat caaaagttcg aattgtcaga tagtgcggat gaagcattaa cgtttttacc
     5221 taatagttta acgatttcat cgaatggaga aaagctgaca gaaggctttg tcatacacaa
     5281 gaaacctcac ggatttgatg ttttattttc gatcccttcg ttggaaaaat atgctggaaa
     5341 aaaactgacc atttcttatc agatgcagct aagcagtaca gcacaggcga acaaggaaat
     5401 caacaacaac ggaacactgg attttggttt tggtgtcagt acaaagaaag tctctgtata
     5461 tacagggagt aagcaatttg tcaaaatcga gacaaataaa ccagataaac gattagctgg
     5521 cgcagtattc cttattaaaa acaaagcagg aaattacctc cagcaaacag ccaacggata
     5581 caagtggaca aagaacgaat cagatgcgct tcacctgatt tccgataaaa atggcgcttt
     5641 ttcaatttcc gggttgaaaa caggaagtta tcgattaaaa gagatcgaag caccttctgg
     5701 ttatatttta agtgaaacag aaattccgtt taccatttca acttttcttt ctgaggataa
     5761 agaggcggac agtatattga aagtagtcaa taaaaaagaa aatagccgtc catttcttcc
     5821 aaaaacaaac gaaacgaaaa atacactttt aggcgttgtt ggtatggtat tcgcaagctt
     5881 tgcaatctgg ttgtttatca aaaaaagaac aggagtgaaa aaatgaaaaa tcataaaaaa
     5941 ataaacgtta tgttaggagt ccttttcctt attttaccat tactcacaaa cagcttcggc
     6001 gcaaaaaaag tgtttgcaga ggagacagca gctcaagtca tccttcataa aaagaaaatg
     6061 actgatttac ccgatccttt aatccaaaac agcgggaaag aaatgagcga attcgatcaa
     6121 taccaaggat tagccgatat ttcattttca gtttataacg tcactcaaga attttatgcg
     6181 caacgagata aaggagcgtc cgtggatgca gcaaaacaag cagtccagtc tttgactcct
     6241 ggtacaccag ttgcttcagg aacgacagat gctgatggaa atgtcacttt atctttacct
     6301 aaaaaacaaa atgggaaaga tgcagtctac acgatcaaag aagaaccaaa agacggagtg
     6361 tcagctgccg caaacatggt tttagctttc cctgtatatg agatgatcaa acaagcagat
     6421 ggctcttata aatacgggac agaagaacta gatactatcc atctctaccc taaaaataca
     6481 gtcggtaatg atggaacgtt gaaagttaca aaaatcggta ctgccgaaaa cgaagcacta
     6541 aatggagcag aatttattat ttctaaagaa gaaggaacac caagcgtcaa aaaatacatc
     6601 caaagtgtca cagatggatt gtacacttgg acaactgatc aaaccaaagc caaacatttc
     6661 attactggtc attcttatga catcggcaac aatgactttg ccgaggcatc tattgaaaaa
     6721 ggccagttga tcgttaatca tttagaagtt ggaaaatata atttagaaga agtaaaagct
     6781 cctgataatg cggaaatgat tgaaaagcaa acaatcacgc cttttgagat cctggcaaat
     6841 agccaaacac cagtagaaaa gaccatcaaa aatgatacgt ctaaagttga taaaacaaca
     6901 cctcaattga atggaaaaga tgtcgcaatc ggtgaaaaaa ttcaatatga gatttctgtc
     6961 aatatcccat taggtatcgc tgataaagaa ggaacgcaaa acaagtacac aacattcaaa
     7021 cttatcgata ctcatgacgc tgctttaaca tttgataatg attcttcagg aacgtatgct
     7081 tatgccttat atgatggaaa taaagaaatc gacccagtaa attattctgt cactgagcaa
     7141 acagacggat tcacggtttc agttgatccg aattatattc cttcattaac tcctggcggt
     7201 acattgaaat tcgtttacta tatgcatttg aacgaaaaag cagatccaac caaaggattt
     7261 tctaaccaag caaatgtcga taacgggcat acaaatgatc aaacaccacc gtcagtcgat
     7321 gtcgttactg ggggcaaacg atttgttaaa gtagatggtg acgttacatc agaccaaaca
     7381 cttgctggag cagaattcgt cgttcgtgat caagatagtg acacagcgaa atatttatcg
     7441 atcgacccat ccacaaaagc cgtcagctgg gtatcggcga aagaatcagc aacggttttt
     7501 acaaccacaa gtaacggttt aatcgatgtg acaggtctaa aatatggcac gtactatctg
     7561 gaagaaacga aagcgccaga aaaatatgtt ccattaacaa accgtgtagc atttactatc
     7621 gatgaacaat cttatgtaac agcaggacag ttgatttctc ctgaaaaaat accaaataaa
     7681 cacaaaggta cacttccttc aacaggcggt aagggaatct atgtgtatat cggtgcagga
     7741 gtagtccttc tactgattgc tggactgtac tttgctagac gcaagcacag tcagatttag
     7801 tctttttgca agaattggaa aaaggagcag cggattacgc aaatgctcct ttttctttta
     7861 ttatcaaaca gtcatcacaa ggagaacaca aatgaaacca aagaaaagga aacttctaga
     7921 tctgctgatg cttttcttgc ttctctcagg aatagggata ctagcttatc catttgttag
     7981 cgacgcgctg aataattatc tggatcagca gatcatctct cattatcagc aacaagcggt
     8041 caaagaaaat gaagaagtca tggctaaaat ccaagaaaac atgacaaaga aaaaccagca
     8101 attagcaaaa gaaggtggaa atccaggtgc agatcccttt actaagaaaa aagaacctgc
     8161 gaaaaaggat agaacctatt ttgagaaaca tacgatcggt attttgacga tccctgagat
     8221 caatgtccat ttgccaattt ttgatgaaac gaatactgtg ctgctagaaa aaggtgcctc
     8281 attattggaa ggaacgtctt atcctacggg gggagaaaat acacatgccg ttatttccag
     8341 ccatcgagga ctgcctcaag caaagttgtt cacagactta ccgaaactga ctttaggtga
     8401 ttctttctat atcgaaatca atggacggac actcgcttat caagtcgatc agatcaagac
     8461 ggtagaaccg actgatacaa aagatctgag aatcgaaaaa gggcaggatt tcgtgacact
     8521 cctaacttgt acaccttata tggtcaacag tcatcgctta cttgtcagag ggcaccgggt
     8581 tccttatcat gcaaaagaag ttcaaaaaga agtacagaaa gtatccgaga acaaacgatt
     8641 tttcctgtat ggattgttag ctgcatgtat actgattttc ctccttctta tatatctttt
     8701 tcgtcaatcg ttcaaaataa gaaacagaag ttagctattc ctcatttcta taatatatct
     8761 attcgaatcg ttccgtaaac aaacaggctg aggtttgaaa aagaaaactt cagcctgttt
     8821 ttatgatgat atcaaagaga gcctatcaaa cactatcttt cttattacta aatgaggatc
     8881 ttttcttaag ttttccgata ttctttaaga aggccttttt gcatcaattc ttgtttgttt
     8941 ttcaagttgc ggatattctc ttcaaaattt tgagaatata tcactgagaa taacgtattt
     9001 aatacgtaat cgaaggcaat ttgagaggag aaagtcgcta ctttagcaaa atcatattcc
     9061 tcctgcgata taacaagaca catatcagcc tgtttggcca tttctgaatg ctgatttccg
     9121 gtaatcagca aggtaggggc ctctacatgc ttcaaatagg tcattattcg ttcataatga
     9181 tggatgcgac cgctgtagct aataaaaatg gcgcaatcct cgctagtcaa attggctgca
     9241 ttccaagaag aatcactgta ttcttcagcc atgatcaaaa acttattcaa cttgaccatt
     9301 ttattctgga atttccttgc cgtgatttga gaatcacctt ttgcaaaaag aaagattcgc
     9361 tgtgccttgt tcaatacttt accgatttga tctaaatctt catgttcaat ctgcaattgt
     9421 gcttttttta ttgactggat cgataaatcc gccattttct ttgcaatttg ctgagtgcta
     9481 tcttcacttg aaaaaggaaa attaggatca atcgtttgat tgaactgagc aatcaattga
     9541 tgaacttccc gccccagctc gaaacggaat tctttaaatc cgctgaatcc catttttttg
     9601 cataaacgaa tgatagctga atgggaagta tacgtagctt ttgctaattc ttcaatcgtc
     9661 atgtagactg cagcttcgat attcctacgg atgtaatcga caatccgttt ctctgtcgtg
     9721 gtaaacaact cttgttttgt tagtttttct tcaataagca tgatctgacc tccaaattct
     9781 atcttactga taaacacttg gttccgctac tatctttttg atttttatcc ttactttgaa
     9841 cattaatttc accaaaccga atgataacaa gtgtttttct gaactttttt accagaatag
     9901 ctattataaa tactttctgc gcttttctag gatatactga aaacatcaat aaaaggaggc
     9961 accactcatg cttacaatcg cttatcttgg caatggaaag agtacaaatc gctatcattt
    10021 gcctttttca agaaaactta cgaacaaaat caatatcaaa aaaatttttt ccccatctga
    10081 accagtgtgg aaaacattcg aagacatcga atatactgat cagctgtctg atctttggga
    10141 tgatcctgag atccaattag ttgtcatcac tacacctagt caatttcatt atgaatatgg
    10201 aaaaatggca ctcgaacatg gtaagaatgt actagtagag aaaccttttg cagaaacgtc
    10261 aaaggaagca gaagagttat ttgcattagc aaaagaaaaa ggtctattta tccaatgtta
    10321 ccaaaacaga cggttcgatt ctgacttctt gacagtccaa aaagtaatcg aaagcggaaa
    10381 attaggggat attttagaag tagaaatgca ttatgattat ttcagaccgg aagtaccaga
    10441 aaatacgcat gaattttcta ctgcgaacag ctatttatat ggacatgctt gtcatacagt
    10501 agatcaagtt ctttcttatt tcggcgaacc agatgatatc agctatgatg tacgtcaact
    10561 tttagggaaa ggacgaatga atgattactt cgatctggat tttttctatg gaaatatgaa
    10621 aatttcgata aaatccagtt atttccgcat caaagaacgt ccaagtttcg tcgtttacgg
    10681 aaaaaaggga tgttcgtgaa acaaacaaaa gatcgacaag aagaacatct gaaaatgttt
    10741 tacatgcctg atcatgaaga ctttggtatc gatttgcccg aacattatgg tgtattgacc
    10801 tatatagatg aacaagggag ttatcatgaa gaaaaagtaa tttccgaggt tggtgattac
    10861 agccgtgtct acgaagggat ttacgagaca ttagttaatg gcgcagaaaa agtcatcaaa
    10921 gatgaagaaa caatcttaca aatgaaaatt ttggaagaag gaatcaaaag cattcaagaa
    10981 tcagaaggaa aaatatgaca tatttgtaag aatcgggttt gttttttgac gcataaactg
    11041 tcttattgta cgatgaaaaa gtatagaact actaggagga accaacatga caaattatga
    11101 cggtgaaaaa cacaaacgat taagaggttc aatggcccct aaaacagaag aagaagtggc
    11161 tttagcagga actaattctg gtgaaacaga agacaaagaa gaatcattag ctggcacaaa
    11221 ctctggtgaa acagaaaata aagaaacagc tttatctggt tcaaattctg tagaagagga
    11281 aacaaagcct aatgagccca atcactttga aaaagatatc gaaaaagcgg ttgaagatgt
    11341 caaagaaaaa tttgaagaac tgacctcaaa aaataaacat gatcatcatt gatcggacga
    11401 aatcaattca taaaagcagg agacatttgg tcacctgctt tttttctgta tccattcgat
    11461 agaaatcgtt cggtacagtc gtaaaaaaaa tccaactgta agttgtacca aacacccctc
    11521 gatttaaaag cgctaccata ctagaatata agtaggaggt gaacagcgct gtgaactaca
    11581 aaattagtta tctcctgaaa acaccaagag aaatcagtct agaaaaagca gcagagatca
    11641 tcgaagtaaa tacaagcgag gcgatcaaac aattaactcc ttttgccgaa tatgtggatg
    11701 atacccgtat ccgtataaag aaattccgag caatcgactg ggcagaaaaa tttttggata
    11761 ttactcgatt tgaagctgtg tataccgaac aagagcgaag agatcttctt tatttgatgg
    11821 tttattcgac tgcaacgtct gtttctgttt ttcattttca agattttctt caggtttcta
    11881 aagggacagt cctgtcagat atcaaaaaga taagaaatga attagaagtg gaagattgtc
    11941 agctgtcgta tactaggaaa actggtttta ggctgctagg gtcagaagca atattaagaa
    12001 gagaagcaaa aaatcgcact gctcgtttga tgcagtcact tgctggaaga tttgggctca
    12061 cgtatttaac cgcaaccctt tccattgatt attacgccaa agttagagat ttgctagata
    12121 aaacgatttc actatcagat cttcgattcg caccaaatcg aatggatgaa ttagcttatt
    12181 atttgagttt gacaaccatt cgaattggaa aaacatccat ggaactggta aaagatgctg
    12241 aactgttggc ttctcttgca gtcactgaca ttagtcgaca agtattgact ggccttggct
    12301 attccgaaca tcaaaattcg gagattatat ttttctctct ttgcttagca tcgatcgttg
    12361 aaggagatgt ccaggagcct gcacttgatt ttttgctgga atgttcttca gaagtcatcc
    12421 atcgcatgga attcctgatg gccgtagaat ttgcttcgtt tcgtgaatta ttgatgaatg
    12481 tcttttatca cttggtccct gcctattttc gtatttccta ttctttttat ttacctaatg
    12541 tgatgatcga tcaaattaag catcaatatg cttccatcta cgaaatgaca agaaaagcat
    12601 tacttccgtt agaaagacga atagggaaga gcatccctga agaagagatt ggttttttca
    12661 ctatactatt tggcggagag atcagaaaag cggacgcaga agaaagaaat agaaaaataa
    12721 gagccgtgat cgtctgtcca agtggcatta gctcttcatt gatcttaaaa agtgaacttc
    12781 agcaattatt cccgatgatc ttttttacag agactaattc agctgaccgc ttacacgagg
    12841 ttgacgaaac ttcttacgat attatttttt ctactgtacc tctcacactt actgataaga
    12901 aaaagtctct ctatgttctg catccaatca tgactgactt ggaaaaaaat cagctgatca
    12961 atcaagtaca aagagattgg ctgataccag gattttcaat gccttctgcc caagaattgt
    13021 tgtcggcgct actgccttat atcgaattaa aagaaggcgt agacgaagaa aagctctatc
    13081 aagtattaaa tcgtaaaatg aacaaacttt tagaaaagaa ggaggatcaa cgtcctatgc
    13141 tatctgaatt attaacagaa gaaatgatcc aattatcgga tcaaccaaaa aactggcaag
    13201 aagcaatcac atttgccgca cagccgctat tatcagaaaa taaaatagaa gaaagttata
    13261 tcaaagctat gatcgagcgc gtggagcagt atggtgcgtt tatccatatt ggcgatcaca
    13321 tcgcattacc tcatgctcgt ccagaagatg gcgtaaacga agtagggatg tctttgctga
    13381 aacttcaaca acctgtggat ctgcttgata atccagccca tcccattagt ttgttcatct
    13441 gtttagctgc tatcgataat gaggcacatc ttcgggcttt ggcaaattta acaaagctac
    13501 tttctaataa acaaaatctt caagatttat tacacgcatc aacaaaagaa gaaatattag
    13561 cgatcataag aaaaggagac gaatgatatg aaaatttttg cagtatgtca aagtggatta
    13621 ggaacaagtt ttatggtaga gatgaacatc aaacaagcac ttgcagatct tggagcagat
    13681 gtggacgagt ttgaagtaag tcatacggat gttggaagtg tatctagcga tatggctgat
    13741 tatttcttca ttgagcattc tttgaaagat acgctaactt ctatacctga tgaaaagctc
    13801 gtacctttgc aatcgatcat tgacagcgaa gaagtaaaag aaaaagtaac gaagatcttg
    13861 tcaaaagaat aaaaatgatt caagggagga actatcaatg gataaattgt tgaatttgtt
    13921 tgtgtcaatc gtcagtcagc cctccatcct cgtttctttg attgcactgc ttggtctcgt
    13981 tcttcagaag aagaaatttt cagaagtgat ccaaggaact atcaaaacat ttgtcggatt
    14041 cctagtactg atcggcggtg ctggtttaat cagtaattca ctaacccctt ttgcttcgat
    14101 gttccaaaca gctctgcata ctcaaggagt agttcctagc aatgaagcag tagtggcgat
    14161 tgctttgcaa caatacggta cacctactgc tttgatcatg ctagtaggga tgatcgtgaa
    14221 tattttactt gctagattca caagatataa attcattttt cttacaggtc aagcaatgct
    14281 ttatgtttct tgtctaacag cagtcatttt agtaagcgcg gggtttactg caaatttgtg
    14341 gatgatctta ttaggcgggg tcttcgaagg aacactatta actattacgc ctgctctttg
    14401 tcaaccgttt atgaagaaca tcactggaaa caatcaagtt gcgttagggc ataccggaaa
    14461 tatcggttat gcttctagtg gatggatcgg aaagtttttt ggaaacaaag accgttctac
    14521 agaggacata aagattccta aaagtttcag ctttttgcga gattctactg tatcgatcat
    14581 gctcttgatg tcgatcgtat atgtcgcact tgctttgtta gctggtcctg gatttgttga
    14641 aagagaacta agcaatggag acaacgctgt aatctttgca ctgatccaag ctggaacatt
    14701 cacagctggg tttgttatcg tccttcaagg ggttcgaatg gtgttggcag aaatcgttcc
    14761 agcattccaa gggattgcta aaaaactcgt acccgattct aaaccagcat tagatgttcc
    14821 tatcgttttt ccatatgcac ctaatgctgt attaattggc ttttttgtta gcttctttgt
    14881 aggaacacta tccatgtttg ctatgatagg cttgcaaact acagttatca ttcctggggt
    14941 agtaggacat ttcttctgcg gagctgcttc tggtgtcttc ggcaactcga ctggcggtag
    15001 aagaggggcg gtattaggtt ctgctttcaa tagcttattg attagttggc tgccattatt
    15061 tatccttcca gtattaggag atcttaaaat cgcttcttct acatttgccg atactgatta
    15121 tctagtacca ggacttgtac ttgggaaact aggagaatac ggtcaaatag ccttgacagt
    15181 tggaatcatc gctttctttt tacttgtcgt attatctagc tttctttttg ccggaaaaag
    15241 aacaaacatt gtttctaaag aacagaatta agcaaacttc aaaataaaaa ttgcttggac
    15301 aatatcagaa gaagatttaa agtcttctga tatgtcgcaa gcagtttttt gttttatgca
    15361 tttatttttg aatagcttgt taagacaagt ggctgttttg ctagaagagg ataactttta
    15421 ccattcattt tcactgttcc gcctgttcta tcagaagtaa tcacgatctt atcttttgtt
    15481 ttttcaagcg aaatctttcc atctttgata ggtagcgtac aattcagtgt ttcaaatgaa
    15541 tctagattag gttgtatcat aaattcttca taacctggct ttgttggata caaaccgata
    15601 aaatagcgtc ctaaaagata gattgggctt gctccccatg catggcataa gctttttcca
    15661 taaggatctc catacatact gtacttttca tttcctgttt gagtagggtc gtattcttcc
    15721 cagaaagtta ccgcatcatg agataacatc cctccccaat aatcaagaat cgtttggtag
    15781 acgatattcg tctgtccgag tttgcataaa gcatcttgtt catagaattt gaaatatgga
    15841 gtagtaatct gtgtgatagt gtcattcaat aagacattct ttaagatcga ttgctgttgg
    15901 tctttgttag caaaatcgaa taaaacagcg aaaatattcg catgacgcgt cacatttctt
    15961 ctcccagatt catagcaatc gataaaagcg ccaagttctt catcccaaaa gtagtcaaat
    16021 aggttttgtt tcaatagttg atatttttct tgatagagat gagtatgata attttcgttc
    16081 tctccatatg tttcagctaa gatatctccg caagtgatca tggaatgata ggctttcagt
    16141 aataagactt gttctgcagc aagcgttcct tctttatcca tttcagacca atcaataaat
    16201 atccaatctt ttgctctccc ataaataaac cctaattcat tcgtttgctc catcaagtat
    16261 ctcattaatg attgcatctt agggtagacg atgcgtaaaa attcatagtc tttcgtcatc
    16321 atatagtggt tttcgatacc aatcaaccac aagatcgaat agtcaacgat agtattcata
    16381 tgctgcttga tttcattttg tcctcttaat gctaatatcg ttcgtttatt gatttcctcg
    16441 tcaaaaaaaa gatattgatt gatcatgaat gattgatacg catctccaga ccaaatccag
    16501 cgatctcgtt tgataccgtc gataaaaaac aaatcactac ataacttgaa ggcttcttct
    16561 gatactgacc agatctgatt cagtaaagaa gaatcgcttg aaaaagcagc ttgtttcgaa
    16621 agagataaaa attcatgaaa aacttgaagt tgaaccattc cagtagaaac ctctggtata
    16681 aacaaatagc gaaaagcgcg tttacgagct ttcatttttc ttgtcattcc tatttgctta
    16741 tagtagcaat aatcgatatc catagcttct acatcggact ctccataaca tagagtgata
    16801 gattcgatat ggtcatccag agatattagt tctacacagc cattcactgc tcgcccaaaa
    16861 tcgaataaga ttcctccgcc aatctccgtt tcagtgactg gatggacgca ttctttttga
    16921 taatagattt gatttggatc ttttgcgata tcgacataaa gcggactcca gccagctgga
    16981 tattcttcaa taaaattact tgctgtccag ccaatatctg attttacttc gtctccctcg
    17041 ataaacatcg ctggtagccc tgatgtatgt cctacaaaga tacgaatttt tgctttacca
    17101 gctggacaag tcagccattt tccacatgga tgtttttgtc cattaatttc tacataccca
    17161 actccttgga tcgtcacttt aaacatggaa ggttgatcaa gaaagtaata tctcttaaac
    17221 ttgacgttcc gatggcaatc atccatgtac cagtacgctg gccagtcaaa acctcgttct
    17281 tcccgttgga aattttgtag cagtccatgg cggatctcaa agtctccagg ataccaaagc
    17341 catctatctt tatgtttttc atttgtcatt ttacacgacc tccatcaaat ttattttgtt
    17401 gtcaattgtt cttttatttc ttcatctact cttatcactg tctttctatt cgtcctgtgt
    17461 actactagaa ggctaataag cgcgctaatc aacagcaaga tactgccatt gacgaaaaca
    17521 gcaagtgttt tactcccaag agaagcaaaa gagagaaaga gcaaaccata tggtgcaatg
    17581 aaagtaccta aattagcgcc aaccaataag attgaagtat tgaaactctc cgcgtacttg
    17641 ggcgcttgga tacttacttg gttgaataag taaggaatga ataacgaaaa agaaattcct
    17701 gttaggatcg caccaaaccc aatcacatac aggtttatca cagtggcaat caatacaaag
    17761 ccgattccca tcagtagtaa agaaagtgta agcatatagc gatgtgcttt tgtctgaagc
    17821 cagccaaaac aaactccggc agccatactt gccaattgca tgattgttaa gatattcgct
    17881 gcattagttt cgtttcctag agaataagtc gtcaggactg tggctaattt aatcgataca
    17941 gcattatata ccacgatgac cagcaagaga aaaataccat aactaatcat tttaggattc
    18001 gttttgatta cttgtcttgt tggggctgtg ccatgcgatt gaagttcagt tgtttcaggt
    18061 gtaggaacaa acaaaacaaa caagacaaga ataggtaagc tgatggcgta gactaaaaag
    18121 gtatattgcc aaccgaactg tactagctgt cctgcaacaa atgtcattaa agctgcacca
    18181 aatccttgaa atgcactttg aaaaccaatc atagaggctc gttcatctcc gtgataaaag
    18241 gcagcaatca tgctgactgc caaagaattg atcaatccaa agccaatacc tagtccagct
    18301 cttgaagcta agacaatact gtaactttgt gagaagaggg ggaggatacc acaaatcgat
    18361 gcaatcacta atccgatgat cacagtttgt ttttgtccga ttttcctagc aataaaacta
    18421 ctaatcaaaa caaaaatagt gaccataaaa gagggagcag tcgttaataa ttcaacagca
    18481 gataaagaac gcgtggcata agtagctgac atactgggaa ttgcagacgt gactgcacca
    18541 gcagaagtca aaataagaga gactgacagc agagaaaatt tcatcatctt tttattcata
    18601 agcatgttcc tttcattttt gtaagcgctt atcaaaaata tagtatgaag tggttattct
    18661 ctcaatagcg gaatcacagg aagttcatgt cccaaccata gaaagtcttt atgaaaccaa
    18721 aaatagcttt gcatataata gcagtacagc gtgaactaat aagcgaaaga aaccgcttac
    18781 aaaaaggagg gagtcaattg gaactagctg aattagacat ttttttgcgt aacgaagaat
    18841 caatcgaaac tctgcagcgt gaacaaggaa tgaatatcaa tgatttaaac ttagcttacc
    18901 atcttcagac accgccaaaa atccaccgag gggatctatt tcaacaggga aatattcata
    18961 tcagtaaaca ccgtcgatat gcggaagttc ccgagcatac acatgatttt gttgaaatca
    19021 attatatgta tagcggtaca tgtttacaaa ttattaatgg agaaaggata gagatggaag
    19081 aaagtgacgt ggtgatcatt gataaagaag ctactcaaag aatcgaatac acagggcaag
    19141 aggatatttt aatcaacatc ttattaaaag aagaaacctt atctacagag atattgaatc
    19201 atttggcaag cacaaataat ctactttctt cttttttgat aaatgcatca aaagaaaaag
    19261 gggaacatac acaattcatt cattttcgcg gcggaaaaac aagtcgtttg acgcatttga
    19321 ttgaaaatat gctgttgatt tattttgata catacagtca taagatccgt tcgttaaatc
    19381 tctatacatc actattactg atcgaattga ctcatttatt agaacaacaa agcttttcat
    19441 cagcagatgg atgcgcagaa aatgagatgc ttagagtatt agattatatc gataagcatt
    19501 ttacttcttg tacgttaaaa gaaacagcag ctcattttgg ctacaatccg gtctatctta
    19561 gtaataaatt gaaaatgtat tttgatgctt cctttcaaga actggttcta aagaaaaaat
    19621 tcgactttgc gttggaactg attcaagaaa cgagtttttc gttggaagaa atcgctaatc
    19681 gtatcggata taaacaaacg acttctttat tcaaattatt caagaagtat tcaacatgta
    19741 ctcccaatga atggagaaat aaacattaag aagaaaatct ttcactaaag catatgaagg
    19801 aaaattgttt taggtgcgat tgctgtctta ttatatcgtt gcagcgaatt tttttggatt
    19861 tattttcagt tctaaacttt tggttgtatt atcaggggat ataggtgtaa acatttgtca
    19921 tttatctgtt atgcgtattc aggaattcgt ttccatcaaa aattataata acaactatta
    19981 taattaacat gttgataaaa tgaataaaca agactatact tttcatgaaa gtaacaaata
    20041 gttatgaaaa ggagctgttt attcatgaaa aaaacaagtc gtaaagtagt aatcgtcggt
    20101 acaggttttg ttggaacaag tatcgcttat gcaatgatca accaaggaat ctctaatgaa
    20161 ttagtattga tcgatgtgaa tcaagaaaaa gcagaagggg aagcattgga ccttttagat
    20221 ggtatggctt ggggcgatga aaatgtggct gtatggtcag gtggctatga agaatgtaaa
    20281 gatgctgata tcgttgttat cacagctggt atcaaccaaa aacctggtca atctcgttta
    20341 gatttagtta aaacaaatgc gtcaatcatg cgccaaatcg taaaagaaat catgggatca
    20401 ggattcgacg gtattatcgt tgttgcttca aacccagtag atattttgac atatatcgct
    20461 tggaacgaat ctggtctgcc aacatcacgt gttatcggta ctggaacaac attggataca
    20521 actcgtttcc gtaaagaaat cgcattgaaa ttaaaagttg acccacgcag cgtccacggc
    20581 tatatcttag gtgaacatgg tgattcagaa gttgctgcat ggtcacatac aactgtcggt
    20641 ggtaaaccag tgtttgaaat tgtagaaaaa gaccatcgta ttgcccaaga tgaattagat
    20701 gtgattgctg ataaagtccg taatgctgct tatgagatca ttgatcgtaa aaaagcaact
    20761 tactatggta ttggtatgag tactgcacgt atcgtaaaag ctatcttgaa taacgaacaa
    20821 gctgtattgc ctgtatctgc ttatttaact ggtgaatacg acgaaaaaga tatctttaca
    20881 ggtgtgccat caatcgttga cgaaaatggt gttcgtgaag ttgttgaatt atcaatcaac
    20941 gaagaagaaa aagcaatgtt cagcaaatca acaagtgctt taagagaagt attgaacact
    21001 gtattataag aaacaatgaa tatgaactaa aaatcctaaa actgcttaa
//
LOCUS       DASNNU010000047        21018 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.047, whole genome shotgun sequence.
ACCESSION   DASNNU010000047 DASNNU010000000
VERSION     DASNNU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.047"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1162
                     /locus_tag="U8116_001616"
     CDS             <1..1162
                     /locus_tag="U8116_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN8434614.1"
                     /translation="EKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINIHE
                     EIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPKKE
                     KHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHILDK
                     LFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDKCR
                     VLLMDYRELKGETFDHITSVGMFEHVGYENLEGYFKVVKDLLKPKGTALIHGISRQQG
                     GATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFHQV
                     QDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            1392..2039
                     /locus_tag="U8116_001617"
     CDS             1392..2039
                     /locus_tag="U8116_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN8434615.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            2123..3067
                     /locus_tag="U8116_001618"
     CDS             2123..3067
                     /locus_tag="U8116_001618"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEN8434616.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            3202..4653
                     /locus_tag="U8116_001619"
     CDS             3202..4653
                     /locus_tag="U8116_001619"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEN8434617.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            4674..5510
                     /locus_tag="U8116_001620"
     CDS             4674..5510
                     /locus_tag="U8116_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN8434618.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            5510..6169
                     /locus_tag="U8116_001621"
     CDS             5510..6169
                     /locus_tag="U8116_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN8434619.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            6295..6984
                     /locus_tag="U8116_001622"
     CDS             6295..6984
                     /locus_tag="U8116_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434620.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            6990..7946
                     /locus_tag="U8116_001623"
     CDS             6990..7946
                     /locus_tag="U8116_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEN8434621.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            7990..8502
                     /locus_tag="U8116_001624"
     CDS             7990..8502
                     /locus_tag="U8116_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8434622.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(8758..9306)
                     /locus_tag="U8116_001625"
     CDS             complement(8758..9306)
                     /locus_tag="U8116_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN8434623.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(9395..10576)
                     /locus_tag="U8116_001626"
     CDS             complement(9395..10576)
                     /locus_tag="U8116_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEN8434624.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(10812..12320)
                     /locus_tag="U8116_001627"
     CDS             complement(10812..12320)
                     /locus_tag="U8116_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEN8434625.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            12479..12670
                     /locus_tag="U8116_001628"
     CDS             12479..12670
                     /locus_tag="U8116_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434626.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            12873..13790
                     /locus_tag="U8116_001629"
     CDS             12873..13790
                     /locus_tag="U8116_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEN8434627.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            13783..14856
                     /locus_tag="U8116_001630"
     CDS             13783..14856
                     /locus_tag="U8116_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434628.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            15004..15522
                     /locus_tag="U8116_001631"
     CDS             15004..15522
                     /locus_tag="U8116_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434629.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            15503..15895
                     /locus_tag="U8116_001632"
     CDS             15503..15895
                     /locus_tag="U8116_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEN8434630.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            15971..16906
                     /locus_tag="U8116_001633"
     CDS             15971..16906
                     /locus_tag="U8116_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEN8434631.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            17039..17818
                     /locus_tag="U8116_001634"
     CDS             17039..17818
                     /locus_tag="U8116_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN8434632.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            17811..18128
                     /locus_tag="U8116_001635"
     CDS             17811..18128
                     /locus_tag="U8116_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN8434633.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            18372..19982
                     /locus_tag="U8116_001636"
     CDS             18372..19982
                     /locus_tag="U8116_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN8434634.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            20139..20987
                     /locus_tag="U8116_001637"
     CDS             20139..20987
                     /locus_tag="U8116_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN8434635.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6836 a         3742 c         4205 g         6235 t
ORIGIN      
        1 agagaaaact ttatacaatc aattattttc acgttctttc tcgctccctg tcgaagtaaa
       61 ttattgggac ggtacaacga aacaatacgg agatacggac actcctcccc aaatcaaaat
      121 caatatccat gaagaaatcc caatgaaaga gattacaagt aacgcttctt tggcactagg
      181 agaagcctat atggatcatc gaatcgagat cgagggaagt atccaagcat tgatcaacga
      241 tgcttatcag caagcaaaca gctttttacg aggaaaagat tatctaaaat ggctgccaaa
      301 gaaagaaaaa catacgaaac aaaaaaacaa agaagatatc catgcgcatt atgacttagg
      361 taacgacttt tataaacttt ggctagatcc aactttgact tattcttgtg cttatttcaa
      421 tacacctgaa gatacgttgg aacaagcaca aatcaataaa gtccatcata tcttagacaa
      481 actctttatc aaagaaggcg aaacgttgct tgatatcggt tgtggctggg gaacattgat
      541 gttcactgct accaaagagt ataatgtcaa ggcaactggg atcacactaa gtgaagaaca
      601 atacgatttg attaccaaaa gaataaaaga agaacattta gaagataaat gccgtgtatt
      661 attaatggat taccgcgaac taaaaggcga aacgtttgat catatcacaa gtgtaggtat
      721 gttcgaacat gtaggttatg aaaatcttga aggttatttc aaagtggtaa aagatttatt
      781 gaaaccaaaa ggaacagctt tgatccatgg aatcagccga caacaaggcg gtgcgacaaa
      841 tgcttggatc aataaatata ttttccctgg cggttatgtc cctggtgtta ctgaattagt
      901 tgataatatt acaaaaaacg atttgcaact gattgatttg gaaagcttac gtcgtgacta
      961 ccaattgaca ttagaacatt ggacaaagaa ttttcatcaa gtccaagatc aagtcacgaa
     1021 agaaatgggt gattccttct atcgaatgtg ggatctatat ttacaagctt gtgcagcttc
     1081 atttgaatca agtaatatcg atgtgattca atatttgctt gttcaacctg gaaacaatga
     1141 catcccaatg catcgtgcat aattgaggca aaagtactgt ttaaagacta tttaaaaaga
     1201 agctaagttt tatgatgctc cagaagtgaa cttttttgtc taacttctag gattgtctct
     1261 aaagcttagt ttctttttct ttatattttc ataataaaag tttggttctt taatttttaa
     1321 acatgacaga aaattttttc ttcgttatac ttgtaataga atactatttt ataacaaagg
     1381 ggatcgaatc aatgaaaaaa actgttattt atactagttt atttgcagct ttgtttttag
     1441 ctgcttgtca atcaaatacc aatcaaggca catctgggaa tacgtcttcc agtagcgaac
     1501 agcgctcttc acaggtatcg gactcctcaa caaaagcaag cacatcttcg tctagccaga
     1561 caaaaagttc tagtgaagca agtactcagt cagatatcaa agtgtctgca gctcgtgcga
     1621 tcgaattgtt ccaagataaa tacccagata ctgcaattac tagtttggaa ttagatagtg
     1681 attggggatc ttacttctac aagatagaag gtgtcgatga tcaaaaagaa tacgaagcaa
     1741 gaattgatgc tcaaaatgaa aaaatggaga cagagaatcc agaaactcta gatcgagatg
     1801 aacaaaatgg tcaaaagaaa aaagaagatg gtctggatgt tgataaattg atcacgattg
     1861 aggaagcagc aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca gattgggatt
     1921 tggataaaga tttaggtact acttattggg aagtcaaagt aaaagacggc aatcaaagta
     1981 catcagtcaa gatcaatagc caaacaggcg aagtactgtc aactgaacaa gatgattaag
     2041 aatttcttcg gaaaacaaga gttgattttt cttttagtca tgatacacta gacaaaataa
     2101 cttgtttgaa ggagcgattt acttgagttt agaaacgacc tttgccaatc atatctttgc
     2161 caatcctttg atgaatgcat ctggtgtcca ctgcatgact acccaggaat tagatgaact
     2221 ggctcattca gaagcaggag catttatcac taagagctgt acgatcaatg aacgaaaagg
     2281 aaatcccgaa ccacgctatt ttgatgttcc tttagggagt atcaattcta tgggacttcc
     2341 aaacttaggt ttttcttatt atctagagta tgctcttgcg tatgaaaaag tgcaggaaaa
     2401 ccaaaatcaa ccgttatttt tttccattgc cgggatgagt gtccaggaaa acttggaaat
     2461 gcttgagaaa atagaaaaaa gtgggtttaa tgggatcact gaattaaatt tatcttgtcc
     2521 caatgtgcct ggaaaaccac aactagctta cgattttgag gccacttatg aaacactgaa
     2581 agaagtattc agcatctttt ctaagccgct aggaatcaaa ttgcctcctt attttgattt
     2641 tgctcatttt gatcagatgg ctgatatctt gaatcagttt ccattgacct atgtcaatgc
     2701 tatcaacagt gtgggcaatg gtttgtatat cgatacagaa caagaagcag tagtcatcaa
     2761 accaaaagaa gggttcggcg gcattggcgg agagtatatc aagccgactg ctttggcgaa
     2821 tgttcgcgct ttttatacac gtttgaaacc agaaatccaa atcattggta caggtgggat
     2881 tcggacagga caagatgcat ttgaacatct gctatgtggc gcaagcatgt tacagattgg
     2941 tacagagctt cataaagaag ggccggaaat tttttctaga atcataaaag aattaacgca
     3001 gattatgtca gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat tacgcaccat
     3061 ttcatagcaa agagacgaag gtcatgattg atcttcgtct ttttttataa aaaacactcg
     3121 tctgttcatt cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg ggaaaataac
     3181 agaggcaaaa aggaggataa tatgaatgat gcaacagaat tagcaaaaga gcttaaagaa
     3241 aaagaattta gttttcaaga actagttacg gatatacaga aaaaaataaa gaagcaaaat
     3301 catgaattga atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca cgagctattg
     3361 attccccagt cagaaaaagg gccattggct ggtatcccat ttcctttaaa aatgctcggt
     3421 caagaaaaaa agggatggct agctacttca ggatctcgtt tatttgaaac acaccgagct
     3481 tctcatacat ctaattatgt gcggcaagca gaagcgatag gtctagttcc ttttggacaa
     3541 accaatgcgc cagagtttgg tttcaaaaat attacagacc cagtcatcta tggcccagca
     3601 agaaaccctt ggaaccttga atgtacaccg ggcggatcaa gtggaggagc tgctgctgca
     3661 gtttcatcag gaatcgttcc tttagctggt gctagtgatg gcggtggttc tattagaata
     3721 cccgcttcgt tttgcggatt gattgggctg aaaccttcgc gagggacgat gccagttggt
     3781 ccatatgctt ggagaggatg gcaaggagct gctattgatt tcggcttgac agtttctatg
     3841 agagacacgg aggctttatt ttatgggatg agaagtatcc attccggtgc gccatatcaa
     3901 gtatcgccag ttgagtggca aacacatcca agaaaaaaac ggttgaagat agctttttgt
     3961 gttgattcac cgatccactc acagattagt gaagaggcag tacttgctgt aaaaaaagct
     4021 gtagatttcc ttggaaaaga agggcatgat ttgacagaga ttccttaccc actagatggt
     4081 cgtaaactga tcacgtctta ttatcagatg aatggcgcag aaacagcagc aatgatccgc
     4141 tcgattgaaa caggcatcca gcgagctgtc agaaaagaag aacttgaacc catttcttgg
     4201 actttgttcc aatatggtca gaagctctct gccgctactt acatcgattc tctgcatgta
     4261 tgggatgaag cagcaattgt catggaaaac ctacatcaag aatacgatct attcctttca
     4321 ccaacgactg catttaccgc ccctaaaatc agcgaagatt tgcaaagtga tgcgataaga
     4381 gcacagatgg aacacatcag tgattgttct gaagtagaag gtttagacgt tgtttcttcc
     4441 atgtttgagg agagcttgaa gatcacacct tatacacagt tggctaatct gactggacaa
     4501 ccagcaatca gcttgcctac tcatttgaca aaagaaaaca tgcctctagg aatccaattt
     4561 atggctgcca gaggaagaga agatttgcta tttcaaatcg gctatctatt tgaagcaaat
     4621 catcagttcc atttaccaaa tccatggaaa tagcagaaac aaggagaaga agaatgaaga
     4681 aaaaaagatt gtttgctcta ataactagtg ccttttttat tttaagcgct tgcggtacag
     4741 ctgccgctaa tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa gaattagtag
     4801 tagcgacttc tggcacactg tttcccgcat cttattataa tgatcaaaat gaattagttg
     4861 gttatgatgt agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa atctccttta
     4921 aagaatacaa cgtggatgga caaatcactt ctgtttcaaa aagagaatca gattttgctg
     4981 cgaacgattt tggtatcaca aaagaaagag aaaaaaaatt ctccctttca gagactttca
     5041 aatattcatt tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc cactcacttg
     5101 aagatcttaa agggaaaaaa gcagctggcg aacccaatac tagctatatg cgtttagcgg
     5161 aaaaacacgg agctaaatta gtcacatatg acaatgccac gaatgatcag tatttaagcg
     5221 atgtagcaaa tggtcgaaca gatgttattc taaatgatta ttatctgcag aaaatggccg
     5281 tatctgcttt accagatatc cctgtaaaaa tactggagaa cgtttatttc aatgaaaacg
     5341 aaagtggttt tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc gatggcgtat
     5401 taaaagagat gaaagaagat gggacgacaa agaaaatatc tgaaaaatat tttcaaactg
     5461 atgtttcagt caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga tgtactttcc
     5521 cctaattgat ttttccctca tgtggacttc acttcctttc gtactgcaag gcttggtata
     5581 cacattaggg attggcttcg tgtcgtttct tttaggaaat ttggtagggt tgcttttgac
     5641 agttttaggt cttttggatt ggctgcctct gaatgtattt attcgttttt atttatcgtt
     5701 ctttagaggc attcccgcac tagtcctgct gtttctgtta tatttcgggt taccttatca
     5761 attaagtgcc ttaacggctt cagtcatctg ctttacgatt accagcagtg catttattgg
     5821 tgaaatctat cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg ctggtttagc
     5881 ttctggtttt tctttctttg gggtattgaa atatatcgtt ttgccgcaag cttttcgaat
     5941 ctcagtaccg gcactcagta acgtagctat ggatctagtg aaaggtactt cgttagcagc
     6001 aacaatcaca gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc ggacattcga
     6061 ttatttttct ctctatattt tagttgcact gatttattgg gtcatttgta tcgggatcga
     6121 aaaagtgcaa cagtatttag aaaaacgttc tatccatcag tcaatctgaa aaatagaaat
     6181 ggtgctagcg tggctggcac catttctatt ttttgtttct tgtatattct gcctagtatt
     6241 ttaaaaccat ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga aaacatgaag
     6301 accccgctaa ccatcgaaat agaatcctcg ttataccaat attgcatcga gcaaggcggc
     6361 ttggttgtag aagaagtcac gatgcctgat gaccaaggaa ttgtagatac gcttagctgc
     6421 ttgacaaaat ttgatggaag ccgagaatgg cgatgctatg agctgaaagt gacaaaagca
     6481 gattttcgct ctacagccaa actgtcattt gttggacatt ataattattt tgttttacca
     6541 agtaaactat atgaagaagt aaaaactgaa atccctacag gaataggtgt tttagtgtat
     6601 cgtccgtaca cggttgaaga ggagatgcct gcctctggta catttctgat tgcgaaaaaa
     6661 gcagcaaaga aagagcttgc tgtaccggaa gaagctttga caaaccgctt catggcttct
     6721 ttattcaggg aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata ttttccaaaa
     6781 gatcaattgt acaaagaatt gaaaagacgt tcagaaaatc aagatccatt ttctaacgaa
     6841 cgatattatg aacggttttt agaggaaata gctagtcagg caatcaatga gttgcaagaa
     6901 gaagttatcg cgcttcagca tgattatgaa tatttgaggc aacaacagca gatccgtcgg
     6961 ttgcctacag agccattgga gtgaaaaata tgtactatcc ttatttaaga ggaaaacaat
     7021 ttgacctatt agcattaaaa gaagcactgt cgcgaggatt gctctccaat aaaatccaac
     7081 cagtgatcga accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa ttatttcaaa
     7141 aaaagcacca tccaatcgct gtgatccaaa atccccaagt aggacaattc aaattattcg
     7201 atcagcatgt gcacagttgg acagtcaaag aaaatagctc tgtcgtccct gcacagatct
     7261 taacgccaga aaatctcagt gaagtactgg attcacctcc agcgtttctt gttttcgatg
     7321 gacagcacta cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt gattcgaaat
     7381 ttttgatacc agatactagt cgctttcgca tctggctgcc cgaaaacaaa attgtgatcc
     7441 gtgactcttt tcaaacgaga aagcatgttg aaagttatgc agataaaaca gatgactttt
     7501 ttagtgatga ctatcttttt ttccacgcag acggatatat tggattttct gatttcacga
     7561 tcgaaggtag ccgttatttc gataaagggt tccctagccg agcgcttgct atccatctaa
     7621 cgtatattga tgcttacggc aatatacgcg tgaaacattt tgtctctgac agtaacgact
     7681 ctgctaaaga ccaagcattg aagtttcttg aagcaggaga taaaatgaaa aaatggatca
     7741 tgcgtcacca tcatcaactc ttgattactt caggaatgat tgaattattg gctctttacc
     7801 agcaacaaaa attccctgga ctgggagtgt tgaaaaagtg gtcacttatg catcatttgg
     7861 aacttattag tcacctgcta gatcatccaa aagattggct taaatattat tcaaaagttc
     7921 aggaactata tgaagtgatc caatgattag ataatgtaaa tgaaaaagga atagaaaaga
     7981 ggaaactgga tgtatattcg gcaaacaagg aaagaagaag tagaagaagt ggtagaaatc
     8041 attaatgccg cagcggattt cattgcttcg caaggctctc ctcaatggca aaacgggcag
     8101 aaaccaacaa aagaaacgat tttgaacgat attgaaagaa aagaaagcta tgtattaata
     8161 gtggaagatc aaattgctgg aacagctgca ttagtctcag gcgtggatcc cgtttacaca
     8221 aaaatagatg gtagttggaa agaatcaact ggtccttatt tatcgattca tcgtgtcgct
     8281 ttagcagaaa gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca tttgttgacg
     8341 attgcagcca gcaaaaatat ccaagatatc cgtatcgata cttatccagc taatgaaccg
     8401 atggaaaaga ccatctattc tgctggattc acttatcaag ggatgattga atttcctttt
     8461 gaacacggcg aaagaaaagc atatcaaata atcatcaatt gactataaaa tcatctgagc
     8521 aaagataaag acattaacac tactgaaaaa aacaatcata tgatagccaa gaggttgtaa
     8581 cagaagtggc cagcttcaaa aaacaagaaa gaatttccga aaatgcttct caaatttttg
     8641 tggaatttta gcgtatttta cgaaatagct gctttctgaa actcccttca tcgagaggtc
     8701 gtgacatagc ttttgtcacg atctctcggc ttatcctacg agtattattc gatacattta
     8761 tatcaataat ccttttttta ttttatctgc agcttcttgg aacgctccat cgtttgacac
     8821 gactaaaagt gaggggatag ctttcaaacg tttcaactgt gctaaatctt ctacataaac
     8881 cgccagtctg gctgaaatct gttcagatga atctccacgt tttttcaggc gcgattgcaa
     8941 tgtttcttta gagatcgtaa taaagatagg acggactacc gtcccgaaat actcatataa
     9001 atgccaaaaa ccatttgctg tcaaaacggc gtaacaattt cctactgtca gtttcttttt
     9061 aatttctgct ttactggaac catatttatg ttgagcatat tggtcccact ctgcaaactc
     9121 atttcccttg atcatctgat tgaatgtatt gtcagaaaca aaataataat cctcttgatc
     9181 tttttcccca ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt ccggctgaaa
     9241 aatagcttct gcgagtgaag tttttccaga accagaagga ccgataaata catagcaata
     9301 attcattgtc atttctcctt tgatgattga acaagtgaaa aagctttgga aaatatttcc
     9361 aaagctttta aaagtaactt tgcttttttg tattttatac cagcaagcca gtcacaatag
     9421 cacttaaaaa gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt tttgcgacaa
     9481 gatttccttt ttcttcattt aatcccttca tcgcaccaga aatgatcccg atagaagaga
     9541 agttggcaaa tgaaacgagg aagacagata taattgctgt tgtacgctca gtaaaatgga
     9601 aattaccatt tgctaattct gtcatagcaa caaattcatt tgtaaccagt ttagtcgcca
     9661 tgatactacc ggcatccact gcttctttcc atgggattcc gctgatgaag gcaagagggg
     9721 caaagatgta acctaggatc tcttggaaag tgatgccgaa tatcccgtgg aagatagcat
     9781 tgatcatggc aatcaaagca acaaaaccga taagcatagc agcaaccgtg atcgccacat
     9841 ggaaaccatc aagaatgtat tctcccaaca tttggaaaaa tgtctgttct ttgttttctt
     9901 caatgatcag ctcatcttct ttttcttcta atacataggg atttacaatt gaagccagaa
     9961 tataaccgcc aaataagttc aaaacaaggg cagtgatcac atatttagaa tcgatcagtg
    10021 ccatataaga tccgacgatc gacatagaaa ctgtagacat agccgataca ctcatggtga
    10081 acaaacgttt ctcagatagg aagggtaatt ccttttttat ggaaataaaa acttccgatt
    10141 gtcctaaaat agcagaagct actccattat aagattccaa tttgcccatc ccgttgattt
    10201 tacttaatcc tagaccaaca gctttcataa ataatggaag aatcttgata tagcgtagaa
    10261 taccgataat agcagaaata aatacaattg gcatcaatac ggaaagaaag aaaggtgcac
    10321 ttcctttatt tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca aaagcaagaa
    10381 gatgatcaaa catttttgcc aatacagaaa caaccgctgt accaaaagtt gtacgtaata
    10441 atacgaaacc aagaacaagc tgtaaaacga acatcacaat tagtggtttc aatttgattg
    10501 cttttttgtt actgctgatc aaaaaagcaa gccctaatgt gacaaataaa ccaataatac
    10561 ctaataaata actcaaataa tcactcctat tccaaattcc gttttatttt acctgttttg
    10621 caaataaaat actaatcttt agtgaaattt tcattacttt tttagagtga aacgcattca
    10681 ttttccatta gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa cgtcttagta
    10741 aagtctataa aaaaacgaaa ctatcgatta agctaatagt ttcgttttca acatttataa
    10801 gtaaactttt tctattggta atatagattt tcggaagggt agacaggtgc gcaggtaata
    10861 tcgattccta gttttttcaa tgtttgttca tcatttttat tgaccatcac agtagagtgg
    10921 gcttgtgctc cctcaagttc agctagttta tcataagcaa cttgagcgat tggattcgtt
    10981 actgcactga ttgataaagc gatcaagact tcattgatgc ttagtgcatc caaagaggaa
    11041 tgaagtgtct ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga caataaagga
    11101 atgtcatctg cgatatgggc taaatgtttt actgcattta agataactgc cgctgacgca
    11161 tccatcaatg aacttgtacg cccggtaatg atgacttcat ctggtaattc gattgcaatc
    11221 acagcagaag gatcttttgt aggactttgc gcttgaatct tatctgcata gtcatgggca
    11281 gggactacca cgtttcggtc ttcaggacgt aaaccgactt cttccataat cagtttgatt
    11341 cgattaactg cttcttcgtc gatcaatcct tttttgaagt cgcattctgt ttggaaataa
    11401 cgacggatga tttcttgttt agaggcttct tggacaacgg tatcatccac gatcccaaat
    11461 cctacacgat tcacacccat gtcagtagga gattgataaa cagattcttt accagtaatt
    11521 ttttcaataa tacgtttgat tacaggaaat gtctcaacat cccgattata gttcacagct
    11581 actttattgt aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt tagatcgact
    11641 gtggctgctt cataagcaat gttcagcgga tgtttcaacg gaacattcca tactgggaaa
    11701 gtttcgaact tggagtaacc agctgcatgt cctttttggc tttcatgata cagttggttc
    11761 aagcaagtag cgagctttcc acttcctggg ccaggagctg tcactacaac gattggttta
    11821 gttgtttcaa tatactcatt ttttccaaaa ccatcttctg agacgatttt ttctacgttg
    11881 actggatagt cttcgatttc tgagtgttta tagaccttga tgtttcgtct ttctagttta
    11941 ttgataaaga ctttcgtggc aggctgtcca ctatagcgag tgataacgac actgttgatt
    12001 aataagccat aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc ataagtaatc
    12061 ccataatcac cacggatctt attacgttca atatctccag catagacgca aatcagtatt
    12121 tcagcttgat ctttcaattt ttgcagcaat ttgattttcg catcttcttc aaatcctgga
    12181 agtacacgtt tcgcatgctt gtcgtcaact aacttcccac caaattctaa atataatttg
    12241 tcatagtggt tcactcgttc caaaatatac gctgactgct cttcaatata tttttctgaa
    12301 tcaaaaccaa tttttttcac taggttccct ccttaagtac taaagcattt tttcattttt
    12361 ttgaggattc gtcaactgga agagcgaaat tcctcaaaaa aacaaagaaa atggtttaaa
    12421 ccaacttgtc tagatagtta ttcattggta tgatagtttt tgataaggaa ggtgctacat
    12481 gaaaaaaaga cagaaaaaga aaaacgcata taaacactat atccgttcga tttttaccgg
    12541 ttatgaaaga atgcttgaag atcccgaatt ggaacaattg acgtttactt atctaaacga
    12601 agaaacgcag ctaactcgtg atgagcatca gcgcattcat ttcacaacaa gagatctgcc
    12661 tagtaaataa aaaataagat aaaggtcatc ggattcgatt ctgtcctatc agatggaaag
    12721 actagttgat cgaaaaaaag gctgtaccaa attttggtgc agcctgtttt atggagcatt
    12781 gcccgctctt ttttataaca attttttaca gaaaatggta ctattgaagt attaataagt
    12841 gaaaaacaga tgagtaagag gaggcaagta gtatgaaaga gccagataca aatgcaaatt
    12901 atgagccaga cgaactagag gagacgaacg cggatcagga tacaggacaa gaatcgtctt
    12961 ctttttacga tatcaaagaa aaagcaagca tgaatctttc ccaattatta gaagacttac
    13021 aggcttttga aaaagctgtc cgcgaagaag atatccctga gatctaccgt ttatacaatg
    13081 gccgattgaa tgaagaatta aaaagaagct caaatgaaaa tcacgagatc gatcaattgc
    13141 tgatgcgtaa gatccatgat cgttttttac aggaatttcc ttttatggaa caagtagaaa
    13201 acatctcgcc aacgatgagc tattacaaaa tcggcactta ttaccatgat cggcccacgg
    13261 tcggtattga tgcaagtctg ccagaaattt ttgttttgcc taagatcgat gaagagtggg
    13321 agaaatattc ccaaccggag tcaacggact atgacaaaaa aatcaatgag ctagacgcaa
    13381 aggtgatcac tgctcagaca gaaattgaac gtttagatga acaaataaaa gaaatcaatc
    13441 gacaaatgac tgatttgaac gacaataaag gattcttgaa tcgtaaaaaa atcgaggaag
    13501 aaattcaaga attagagaag aagaagcaag tactatctaa tgaaaaatta ggctggcttc
    13561 cttatattga gacgcctgaa acaatccagc agcaaaaaga agcattgaag caagaagcaa
    13621 gagcagacca gcttcgtgca gccatcgtag aaaaagaaca aaggcaaatc agacgctatt
    13681 tcggtagcaa agagggattc ggtcaagcaa tccatgaatt cttgatgaat tatcttggca
    13741 atgagaatcc taataatcaa tctaatgaag gaggttccga gtatgaatga acacgatgaa
    13801 gcagaatgga caaaaaatga agaacaaaca gaagatcatg aagaaacgaa cacttcattg
    13861 gaagaacaaa caagtaatcc tctggatgac acagaagaaa tccctatgct agaggaaaca
    13921 aaaatacatg aaaaagaaaa agtaactccg acgaatacga atactgcaga aaacacccat
    13981 gcttacgaaa taactgaaaa tcccgggtat ttagaaggaa aagagcaaga tcatcaagat
    14041 atcgtcagtt tgattgaaac agcggaagca gaattagcgg atttacgtat gagaaaagat
    14101 ttgatcgaat cagattttcc acaattatta gaatcatatg atcgttttat attgaatgaa
    14161 gaaccaatct ccacgatcgg aaagacaagc ctgatcgaat acttcacgta tgaattactg
    14221 aaaccgttga atgatattgg tctggaacgg tcaaattcag cttatgacac atggaaaaca
    14281 agacattttt cgatcaatta tacgttgaat ggacaaaacg aattagtctt ttcctttgta
    14341 ttaccgacca tcgatcaacg ataccaagtg gaatcgatga gtctcctaaa cgtatcacca
    14401 gagacaatgg aaatcaatgt ccaagatgac cgggtgcttt ctttgatccg acactggtca
    14461 gtggatcgag tattctctgc agggcagatc acgatattca atcacaaatt gaatcaatta
    14521 ttagcccatg caagaacact tggttttacc gttaatcaga cattactaga caatacgaag
    14581 ccacttcgtt tgaatcttca gagcgaattt gaattgacga atgaagtatt ggatgatatt
    14641 tttatcgtgg caatgaaaaa tccatcctac gatatggaaa agatcaacga aactacttat
    14701 caagtacgat tagataaagg acagtcttta acgataacaa caaacgaaaa agatcaaaca
    14761 gtcttgtcga tctcatctgg agactaccca cgatctgtca ttgatttctt tatcaattac
    14821 gaattcttgg tcccattaat ggtgagaaag tcctgattgg ataagttgga ttaccagatg
    14881 tcattcagat aattctgttc caaaggactt gcggcagtta tccagaataa ttgtcacaag
    14941 tcttttctgt atgtactaaa tggacgtgaa taacttgctg atgaaggagg gatttcatta
    15001 ggaatgtcca caaataaaat agcacagctt agcttattgt ctgctgcttg tgtagcaggt
    15061 aggatcgctt ttcaatttat tccgaatttc cagccaatga ctgctatttt tttatttata
    15121 gtactttact taagcttaaa agatgcgtta attgtcatga gtctttctat tgcgatcagc
    15181 agtttttatt ttggtgttgg accatgggtc atcggccaat ggatcagcta tactgttgtt
    15241 ctgagtttgt tttcaatcgt ctgcttgaga aaaacagtgc aaaggaatct atggttaaag
    15301 ggagtctgtt tttttctggc gggcatggtt tacggaatag gtatgaccgt ttttgatacg
    15361 ctgttatatc gtttgcctca accttggatc tattaccttc aaggggtgtc gtttgatttg
    15421 atgcacagta taggcaatgt tgtgttcttt cttgtttttc tgccagtcgt caaacgtttt
    15481 tacaatcatt caggaggaaa aaatgaaaaa aataatttat agtttgcttg caatgagtgt
    15541 cagtatactc attttgacaa gttgcagtac aacctataca caatcggctt ccaaccaaaa
    15601 ggaggaagta aaaacatcgc aaaaagcaac aatcacgtta caagaaaatg gtcaaaactt
    15661 ttcggaaaaa gaagtggtct tcaaaaaagg cgatgattta ctgagtattt tgaaacgaaa
    15721 ttttgaagtc aaagaagata acggcttcat cacttcgctt gaagggcact cacaggatga
    15781 gaaaaatcaa ctctattgga tgtttactgt ggacgggaaa agtgctacta ctggtgccaa
    15841 agagatcaaa ttgaatgatg gacaatcggt cattttcaat ttgtctaaac tataagtttc
    15901 ataaaaaact gttaaagggc gaatattgcc ataattcagt gagttcagtt gatgaaagaa
    15961 ggaaaataac atggaaaatt ttaaaaaaag cggaatgttt tccgtaattg ctgctctagg
    16021 ggctaatatt ctagttgcta tcagcaaatt tgtcggctat gcaatcagtg gcagtgccgc
    16081 catgctaaac gaaagcatcc acagtatcgt tgattgcagc aatcagattt tgctgttatt
    16141 cggtgataaa caagccaata aaggacagag cgagttgcat caatttggtg aaggacgagc
    16201 aaaatacttt ttcagtacaa tcgtcgcaat gatgcttttc ttcggcggtg gggcacttgg
    16261 ggtgatggaa gcttttgaaa agttgacgca tccatcccat gaagtgggta atccgatcat
    16321 tgttatagca atactgttat ttggtttggt aatcgaagga agttcgttac gcgtggcaat
    16381 gaaagaaatc aaagaattga atactgaaaa actttccacg atgcgctttt tgcgcgaaag
    16441 tcgacacagt gaaatactga ttatttttac ggaagacttg tgtgctgtaa tcggtttggt
    16501 cttggcttta gcaggaacaa tattgactta tgcaaccggg attgctgcat ttgatgcgat
    16561 cagcggattg ctgatcggtt tcttgctgat gggagccgcc attttcctag caaaagaatt
    16621 ttatagtctg atcatcggtg aaagcgtgac agcaagcgat ttatcaaaaa tcaagttagc
    16681 ttttgaacgt cctgagatca gtcggctgat cgatataaaa acagttcatt taagtccaac
    16741 ggagatttta gttgcagcaa aagcagatat cgttggtaac aaagagaatg aagcttactc
    16801 ggtcatcaat gatattgaaa aaaatatgcg tcagtctctt cctgacaaga agatgtatat
    16861 atacattgaa acagataaat atgacccaaa ttattctcgt aaataaaacg tttattcatg
    16921 aattgaagca aaaatgagaa aaatataatc ttgtttttaa ataaaacggc tacaatttag
    16981 cgaatttatg ttacaatgag tccgctgttt atttgttgta attaaaggag atattttaat
    17041 gaacaaaaag aaaagtgcgc catggcttca agctttgcat gcagtgatgc cactttgtat
    17101 aagctatata ccagtaggat tagcttgcgg agtattgttg caaaaagttg gatttgatcc
    17161 tctcttatca ggactgttat cgattctggt cttttccggt ggcgcgcagt ttttagttgc
    17221 atctatgctt acgacgcaag catcatttgc aacgacatta ttgatggttt tctttttaga
    17281 attacgctat acattattag gagcaagtct tgccggtttt atgaaaaaag aaaagcggac
    17341 gtttttggct gtcttttctc aatcactgaa tgatgaaaac tatgcggtga actacttaaa
    17401 gttttcaact gacccttcct ggaataaaag aaaggcttta tatgtcaatt ggttctcaat
    17461 gactgcatgg gccggtagca atatgatcgg taattttttc ggctcggtga ttcgagtaga
    17521 tgctgacctt gtccattttg ctttgacggc catgttcatt ttcatgttta tcatgcagat
    17581 gaaaagcgca ctactgatct ttacaggttt attctcaggc gtattagggg tcgtattcat
    17641 ggtattattc caaaatactt tcggattgat tgcagcaaca gtcattgctt catttgccgg
    17701 atttatgctg gagaaggcat tcaaaaaaga aaaagctaag aaactaagag accgcggtaa
    17761 agatgtcaat gagccgctct tcaacttccc ggaacaggag gccaatcacc atgactaaac
    17821 tatatttgct acttttagtt gcttgtctgt tcatcgtggc atatatcccg cgcttgttcc
    17881 cgatgcttta ttttactcat cgaaaagtgc catcctggtt cagtgactgg atgaaatatg
    17941 taccggtggc attatttgcg gcgttagctt ttaaagatgt tttcattact catgaacatc
    18001 tggatattgc ttggaatatc aaaatagctg caatgattct agtagcaggc gtggcttaca
    18061 aaacacgttc gatggcgttg tcagttttga caggacttgc gtctgtcttt ttgctgtcta
    18121 tgctttaaac tgttttattt tattaaaaac agagattgcg acataagttg ttcaaactga
    18181 tctatccgaa ctgcatttgc aacagtcaat tctggatgca agtttattcg tagggataaa
    18241 aaagattagt gttggcttat gatgcaagct cttttttatt ttaggaatta gagagggatt
    18301 tgtccttcta tgattttttg ctataatagg gaagatgttt attttatgaa agaatagttg
    18361 agggaattta aatgaataaa aaattgatgc aaggaacatt ttggctaacc tttgctaatc
    18421 ttctatgtaa agttttaggg gtagtctatt tgattccctg gctaagtatg atggggaaca
    18481 atcaagatgg tatgcttgct acgacattgt ataatgttgg ctatcttcct tatggattgt
    18541 tcctgatgct tggtacagtc ggatttccaa atgcgattgc aaaaaaagta gctgtagcca
    18601 caaaagaagg agataacgct gcttgtcggc tgatttttag aagtacgatc aatatcatgt
    18661 ttgtcatcgg tatcgcctct gctgcactga tgtatttatt tgctccttta ctagccggaa
    18721 tcagtccgat tgctaacgtt aataatggga tactagctat cagaagttta tgcccatctt
    18781 tgattgctat cccaattctt agtgcaatga gaggatattt ccaaggaaaa aatgatctgc
    18841 ggccttatgg aacttctttg attatcgaac aggctgtgcg agtgatcgtt atcttggctg
    18901 gtacttatta cttgcgcgta ttgactgacg gaaccatatt agaagctgta ttgatcagta
    18961 cagtcgcttc tttcttcggt gggttagctg cgattatcca tatgtatatc gtcggacgca
    19021 gaaaagactt cttcgaactt aaagattttt tgatttccag ccgttatttc gaaagagaaa
    19081 accgatctgc ctctgtctct atcattcggg aaacactacc gtttattttt gtcggctcag
    19141 tcattactat cgttcaaatg atcgaccaag taacgatgaa accactgctt cactttttcc
    19201 gtccagagat cgcagatcag caactggaat tcttattcag ccgtgcttct gtcaatccaa
    19261 ataaattgac attgatcctg atctccatgg taggaacagt agctataagc agcttgccaa
    19321 tattaagcac gatgaagaaa aaagatcgga tacaaatcga aaaaacagta ggagatagtt
    19381 tttcgattgc gttattgatt cttcttcctt cattgactgg aatgtcttta ttagctggac
    19441 cactatacac attgtttttt ggctatgatc ctgaaagtgt cggatatttc caaatggctt
    19501 tgctagcttc tttgtttttc tctttattca cgatcctatc tacgatgctt caatctttga
    19561 atcaccatcg ttttgcgatc cgattaactg tagaagcaat tatcctgaaa gtcatcttcc
    19621 aagtaatcgg attaggatta gttggcggat acgggatgag cttatcaaat ggcttagcat
    19681 tcggtattgt ctttgttcgc ggctatcact ttatgtgcaa agaataccga atagcacctt
    19741 tagcaaagat cagcaatttt ttcttaaaaa ctttccgctc caccctgatt atgcttgcag
    19801 tatgttttgc cgttttcttt ttgctgaatc agcgattgtc aatggaatca aaaagctatg
    19861 caatgattta ttgtgtggct gtaggaagta ttggcggctt agtattttta ttttctcaat
    19921 tcggaaaaaa tgggtttcga atgttgaaaa atgttaaaca tcatagcaga agcaaagaat
    19981 aggttaacta atcgtttttt gctgtcactt gaaaaacggc aactcgtatg attacgcttg
    20041 tcgtttttct attttccaat tttagtttca tatctaatct tacttatgat atagttataa
    20101 ggtctggaaa aattctttga aacgaggaaa ttaaacacat gaaacttgca gtagttacag
    20161 atagttcggc atatctgccg gaacgtatca agaaccatcc cgacttattc gttattccaa
    20221 tccctttgat tatggatggg aaaatttata acgaaggaat cgatatcgaa gcggatgaat
    20281 attatggact tttaaaaaac agtaaagatt ttcctactac ttcacaacct gtcgtagggg
    20341 aagttttggg tttatatgag gaattgaaag agaaagggta tgacacgatt ttaagtatcc
    20401 atctttcttc agggatttct ggctttgtca acacattatt tgcgatgaaa gatgacatca
    20461 gcggagtaac ggtcattcct tatgactcta agatcactag tatgccgatg ggacacatgg
    20521 tagaagctgc cttagactta aatgatgaag gtcagtccct tgatgaaata cttgcacata
    20581 tcgatcgaat ccgagataat acctatgctt atctgattgt agatgatttg aacaatttag
    20641 tacgaggagg acgtttgaca aatggtgcag cgctgattgg cagcttatta aaaatcaaac
    20701 cgatcctgac attttctgaa gggaaaattg ttttattcga aaaaatccgt tcaagtaaaa
    20761 aagcatttgc tcgagctgaa gaaattattg gtagaagaaa taacgaaata caaccgccag
    20821 tcaaacttta cgtgatacat gcaaataatt tagaagtagc gattgcggag aaagagaaat
    20881 tgcagaaaaa atatccaaat gcagaaatcg aaattggtca tttcggacca gtcattggta
    20941 cacatttagg tgaaaaagcc atcggcattg ccatttcagc gcaataattc cttttattca
    21001 aaaaacatac caaaaata
//
LOCUS       DASNNU010000048        20947 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.048, whole genome shotgun sequence.
ACCESSION   DASNNU010000048 DASNNU010000000
VERSION     DASNNU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.048"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..563)
                     /locus_tag="U8116_001638"
     CDS             complement(<1..563)
                     /locus_tag="U8116_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEN8434636.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="U8116_001639"
     CDS             complement(750..1400)
                     /locus_tag="U8116_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434637.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="U8116_001640"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="U8116_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEN8434638.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="U8116_001641"
     CDS             complement(2912..3910)
                     /locus_tag="U8116_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEN8434639.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="U8116_001642"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="U8116_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEN8434640.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="U8116_001643"
     CDS             complement(4926..5657)
                     /locus_tag="U8116_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEN8434641.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="U8116_001644"
     CDS             complement(5661..6926)
                     /locus_tag="U8116_001644"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEN8434642.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="U8116_001645"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="U8116_001645"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEN8434643.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="U8116_001646"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="U8116_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEN8434644.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="U8116_001647"
     CDS             12268..13770
                     /locus_tag="U8116_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEN8434645.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="U8116_001648"
     CDS             13772..14509
                     /locus_tag="U8116_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN8434646.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="U8116_001649"
     CDS             complement(14680..15873)
                     /locus_tag="U8116_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN8434647.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="U8116_001650"
     CDS             complement(15952..16158)
                     /locus_tag="U8116_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEN8434648.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="U8116_001651"
     CDS             complement(16151..17293)
                     /locus_tag="U8116_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN8434649.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="U8116_001652"
     CDS             17805..18440
                     /locus_tag="U8116_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8434650.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="U8116_001653"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="U8116_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18916..19032
                     /locus_tag="U8116_001654"
     CDS             18916..19032
                     /locus_tag="U8116_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEN8434651.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            19048..>20947
                     /gene="tet(M)"
                     /locus_tag="U8116_001655"
     CDS             19048..>20947
                     /gene="tet(M)"
                     /locus_tag="U8116_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEN8434652.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRY"
BASE COUNT         6163 a         4297 c         3720 g         6767 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatata
//
LOCUS       DASNNU010000049        20537 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.049, whole genome shotgun sequence.
ACCESSION   DASNNU010000049 DASNNU010000000
VERSION     DASNNU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.049"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..500)
                     /locus_tag="U8116_001656"
     CDS             complement(<1..500)
                     /locus_tag="U8116_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN8434653.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIIN"
     gene            complement(599..949)
                     /locus_tag="U8116_001657"
     CDS             complement(599..949)
                     /locus_tag="U8116_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEN8434654.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1106..2635)
                     /gene="ahpF"
                     /locus_tag="U8116_001658"
     CDS             complement(1106..2635)
                     /gene="ahpF"
                     /locus_tag="U8116_001658"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEN8434655.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2645..3208)
                     /gene="ahpC"
                     /locus_tag="U8116_001659"
     CDS             complement(2645..3208)
                     /gene="ahpC"
                     /locus_tag="U8116_001659"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEN8434656.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3352..4014)
                     /locus_tag="U8116_001660"
     CDS             complement(3352..4014)
                     /locus_tag="U8116_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN8434657.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4198..5844
                     /locus_tag="U8116_001661"
     CDS             4198..5844
                     /locus_tag="U8116_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN8434658.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5835..6917
                     /gene="uxuA"
                     /locus_tag="U8116_001662"
     CDS             5835..6917
                     /gene="uxuA"
                     /locus_tag="U8116_001662"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN8434659.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7025..8578)
                     /locus_tag="U8116_001663"
     CDS             complement(7025..8578)
                     /locus_tag="U8116_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN8434660.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            8726..10015
                     /locus_tag="U8116_001664"
     CDS             8726..10015
                     /locus_tag="U8116_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN8434661.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEAAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(10141..11220)
                     /locus_tag="U8116_001665"
     CDS             complement(10141..11220)
                     /locus_tag="U8116_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN8434662.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(11329..11769)
                     /locus_tag="U8116_001666"
     CDS             complement(11329..11769)
                     /locus_tag="U8116_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN8434663.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(11826..12236)
                     /locus_tag="U8116_001667"
     CDS             complement(11826..12236)
                     /locus_tag="U8116_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN8434664.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(12417..12851)
                     /locus_tag="U8116_001668"
     CDS             complement(12417..12851)
                     /locus_tag="U8116_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN8434665.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(12951..13799)
                     /locus_tag="U8116_001669"
     CDS             complement(12951..13799)
                     /locus_tag="U8116_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN8434666.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            14188..15954
                     /locus_tag="U8116_001670"
     CDS             14188..15954
                     /locus_tag="U8116_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEN8434667.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            16056..17315
                     /locus_tag="U8116_001671"
     CDS             16056..17315
                     /locus_tag="U8116_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN8434668.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            17462..18763
                     /locus_tag="U8116_001672"
     CDS             17462..18763
                     /locus_tag="U8116_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN8434669.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            18766..19617
                     /locus_tag="U8116_001673"
     CDS             18766..19617
                     /locus_tag="U8116_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN8434670.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            19630..20445
                     /locus_tag="U8116_001674"
     CDS             19630..20445
                     /locus_tag="U8116_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEN8434671.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
BASE COUNT         6833 a         3742 c         3785 g         6177 t
ORIGIN      
        1 aaattgataa tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga
       61 agcatcaaac ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata
      121 ctactataaa cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag
      181 ctgttgatga taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca
      241 aaagcataga cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc
      301 caagaacctg ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa
      361 acaagatagg cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca
      421 atcgcaatcc cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt
      481 tgtttaatcg tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt
      541 cattttacca aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc
      601 aacttctgag gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta
      661 aataacgaat ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa
      721 cacctgtaat caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga
      781 aagtcacttg tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac
      841 aaatgatttc atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca
      901 taggataaac gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc
      961 tttctttttt tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga
     1021 ttattcaaaa aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc
     1081 tctgactttt tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag
     1141 ctgcagtagc tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat
     1201 caccagctgc gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat
     1261 ttcttggatt catttcgatc gttccatcta gccaatccgt attagggagt aagccgacta
     1321 gaatgaatac tcctgaaaca gctagtgtat gcatctccat cgtacggcga tcgatatagg
     1381 agagagattc tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag
     1441 tgacattcgc aagagagact agtttatcct gtaagatttg atctgctttt aattctggta
     1501 agaattctag aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac
     1561 cagaattacc accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt
     1621 gtgggcaata agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac
     1681 gccagtgcgc acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta
     1741 agccaatatt aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa
     1801 tgtcgatagg gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg
     1861 tgtaagaggt accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag
     1921 aatcagcgac aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag
     1981 ctgctggtcc accgccaatt actaatacat cataaggttc tttttcaaac aactcttctt
     2041 caatcaaagg gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa
     2101 attcttctcc atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt
     2161 tttggaacat cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata
     2221 tattcaatgc ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa
     2281 aatgcaatgt ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg
     2341 cccgtccact aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga
     2401 taccagcaaa aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc
     2461 gcatcgaatc tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa
     2521 attcagctag tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag
     2581 attcaatcag tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca
     2641 ccacctaaat ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc
     2701 attttgccgg acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat
     2761 caatcagtgc actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga
     2821 ttccgtctgg atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa
     2881 catcaaaaat acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac
     2941 cgatagcatc tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag
     3001 aaaagacttc aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt
     3061 cagtaggaca aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca
     3121 taagatcttt ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt
     3181 cttcgatttt tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat
     3241 aattattgta atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat
     3301 tactcaaaaa cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg
     3361 gaaatactcg ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg
     3421 caatcccgtt tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc
     3481 ataatgttgt tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg
     3541 gaaacgttct aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc
     3601 aatttcgaaa caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt
     3661 tattttgact gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt
     3721 gtccaatttg gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg
     3781 agcattttct gcagaaacaa gattaatttt ggaaacatac gtaccacttt gaggtatcgt
     3841 atacacgagt tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat
     3901 agcaagatgt tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata
     3961 gataatttgt ttacgaatcg cttgataagc ttgtgattgc aagctgcttt tcatctttat
     4021 tcctccaaaa tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt
     4081 atgtcacatt aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt
     4141 tattgtataa aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg
     4201 attgatttaa acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata
     4261 cctcattttg accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat
     4321 ttcggtggcg gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat
     4381 ctaggcgaat tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa
     4441 agggcttacc aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag
     4501 ttcgtcaaac gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct
     4561 gaacactttg ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt
     4621 gctattactg aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt
     4681 gcagtcgata taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgcg
     4741 ctactttata aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat
     4801 ttttcacaaa acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa
     4861 aaggaaggtt atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg
     4921 tttccatgga cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg
     4981 ttggcaaaag agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt
     5041 gctccatttg tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat
     5101 ggacgtccga aattcgaaaa agctggggta ttcttgactg atcgtgcaac ggtagataaa
     5161 gcagatacga tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg
     5221 ggttgtttac tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg
     5281 ctagtttctg aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt
     5341 gatccaaaga tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct
     5401 gatatgcctc agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag
     5461 acgattaaaa aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca
     5521 ttgactctcg ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc
     5581 atcccgagtc cagacccact attgacagac tttcagcata agctttccga tattcaactg
     5641 ggtacgcaag atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa
     5701 atttttggtg tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc
     5761 aagatggtag caggaccgaa tgccgtacga aaaacattac aggattactt acaagaacat
     5821 ggaggaaatt tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta
     5881 ccttagatga aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata
     5941 taccagtagg agaggtatgg cccagggaga ggatcaaaga actaaaagaa gaaattgagt
     6001 ctcgtgggtt aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg
     6061 gattaccttc ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag
     6121 aatatggaat agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg
     6181 accttgacta ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc
     6241 cagatgatcc aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttgaattgc
     6301 cgggatggga acctgagcgc ttagctcata tcaaatcctt gcttgaagcc tatgcatcag
     6361 tagacgaaga aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat
     6421 gtgaagaggt aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg
     6481 gattacctcg aatcgtaaag aatcgagaag atttagattg gttgtgccgc atagtagaca
     6541 gcccttataa cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg
     6601 tatatgggat tttagcagaa ttttgtcaaa gagatcgaat cccatttgca catgttcgaa
     6661 atattaaatt tttagctaat cgagatttct atgaggcacc gcataaatcc gaatacggtt
     6721 cattagacat gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac gaatacatcc
     6781 gtcctgatca tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg
     6841 atcgagcatt aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca
     6901 aaagtaaaga taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc
     6961 aaattcttga tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt
     7021 tggtctagga agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta
     7081 attcaaagat atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga
     7141 ttccttttaa aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc
     7201 tgataacgat gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa
     7261 aaatttgttc tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca
     7321 caccaatcac aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa
     7381 ccatccattg cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc
     7441 tgaaatccat ataaaattgt tcaatggcgg tttggacttc tttcaacacg ctatgttctt
     7501 gataaagcat gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat
     7561 aaagtttatt tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca
     7621 aatcaaatac agtacaaaag gactccacca tttctcttga ataaagaaca tgaggaacgt
     7681 ttgctttttt taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga
     7741 tagaaagtcg agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag
     7801 gtcgcaaaat atctacagag ggatcaataa aaaagtcaat atattcatac tctgtgaatt
     7861 ctttttgtac gtctttcaca gaagttgcag gaaaattctc tttacatatt tttaatagaa
     7921 aatataattt atctatcata ccgctattta gtcggtcaag tacttctttt ttaaaaaaag
     7981 tattcatcat aaaagccact acttgcggat cttcatcata aaattctgta gcagtacttg
     8041 aaaagatgtt ccaataaata tctaatgctg catattgaat ttgtacctct tttccagaaa
     8101 aagactttct gccattatta gaaaaggaga tagacaaacc gtacaatgct aaaaatgcat
     8161 taatcttttt cattttcgta tacaaataag aggcactata gtttgtttgc tgacttaaac
     8221 tgataatcga atttgtctga cagctgaaca aagaagtcaa gatctgataa ctgactgatt
     8281 cgttgcagta tcttccaaat agacgataat agcagtaagc cggatcaatt ccttcaaaat
     8341 tcaactttaa aaaaggtttt tgcttatcaa tatgcagttc tgttagattg agacgctcaa
     8401 agtcttgttc taattctttg atgtatagta aaaaatttga ttcagacata tggaaatgat
     8461 ctgctaaata ttgtgtagta ataaagaaac gatgttcaga taggaaaaga aaaatatcta
     8521 attttgtatt gctttttttc ttcaagagaa acgttttaat cgcttcttga gtttgcatga
     8581 aagaggctcc tttttactta aattgttaaa acttgaaaat attactgttt ttttcataaa
     8641 tttagacaaa aaaacagtaa acatataggt agaatctcta ctatcttatt tcttgcatac
     8701 aaaaaaatca aggagtgaga gagttatgag atattcaacc atcttaatat caacatttgc
     8761 agccttaagc ttatcgctta ctataaatca gacagtccac acagaggaga tagaaacagc
     8821 caaatggaca gaaaccaagt gggccaacgt caatcgaatt acctttcagg atgaattttc
     8881 atccttcaat tcaggagtcg caaaattaag aaaaggacaa acagcgacca tcaaagcaaa
     8941 aattgactat tcaggagact tagcacctat actacaatca gctgttacat ttgagaatgt
     9001 tgatcccgct ttgtcgattg gctatgacga gaacactttg actatccaac acaagcaagc
     9061 gatatttacc ttcaccgtta cattaaatca ggacttgtta aaaccagcgc tttttactgt
     9121 caaggtatca gatggactac cgaacgatca tcatcattta acgccgtata gtcagcggca
     9181 gacaattgag caagattcag ctatcgactc aggaggagat cttgttgaag aaccaacaga
     9241 taaaccagaa aatgaaaata agccagaagt accgcctaca gaaaatccag acggagaaca
     9301 gaaaccggaa atagaacctg gagaagaacc cgatacagaa actcaaccag agccagacaa
     9361 tgaatcaaaa cctgaaataa cccctggaga aaaaccagat gtagacccag aagaaaaacc
     9421 ggatgtgact ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc
     9481 agacacagat aatgaaactg aaaatccaga aaaaccagag gcagccccag aagaaaaacc
     9541 agatgtaact ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc
     9601 agacacagat aatgaaactg aaaatccaga aaaactagag gtagatccag aagaaaaacc
     9661 agatgtgact ccagaaccag atacggacgc aagagaccaa ggaataccag aaaaaataaa
     9721 caaaaaaaca attcaagagg atggaaaaaa agagagtaaa aaatcaaatc tagcaattct
     9781 aaaaataaat gaagagcagc tgaataaaaa atcacgaata tttgatagcg cccaatcagc
     9841 tgaaacctta aaaagtagca aggatacaac ctttgctagt cctgaaacga aaaataaaca
     9901 gctaccaaaa tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt
     9961 aagtatagca acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt
    10021 gaataatgtc tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa cgaccccaaa
    10081 ataccagatt gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt
    10141 ttaagggata tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc
    10201 taaaaagaag gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc
    10261 gctaacaagt aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa
    10321 cttcggattc agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata
    10381 aacaacatct cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat
    10441 gataacacct gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag
    10501 ctgatgaaag ccaaatacag ccaaggcgat tgtagtgagc aacgtgttta aaagagcgat
    10561 aacaaattga gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta
    10621 aatatcttga aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt
    10681 ttttttcttt tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc
    10741 catttttcct atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc
    10801 aaaaaaatcg tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt
    10861 tggctgatgc tcgtaaaaat caataacgga ctggatcata tcaaatgttt gatgaataag
    10921 catcgggata tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat
    10981 gatagctaaa acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat
    11041 catcaaataa gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa
    11101 aagaacaaaa acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa
    11161 acgctgataa acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat
    11221 tatatgatgg aatatgaaaa taagaaagag aaagagcaaa aagaaaaacc gctaaatgac
    11281 gttatcttat gagttggttt tcctcataaa atcgttcatc tagcagtttc agcccttttt
    11341 ctggtcttga tgaaaattat ctagcatttt tcgggtcaga tgttgcagtg tatccaattc
    11401 ttcctgttca agtggaagat aagacttgat cttttgtgga ataccttctg cttgtttttg
    11461 caacgcagca ccttgaggag tcagcgtaac atttacgatt ctttcatcac ccgtttcacg
    11521 ctgacgatta atataatttt tttctgctaa cttttttagt aagggagtca gcgtaccaga
    11581 atccagacac aaatcatttc ctagctcttt cactgaagtc tttttctttt cccataaaac
    11641 taacatcaca agatactggg tataggtaag attcagcggc ttgaggaggg aagtgtagtg
    11701 tctaatgatt tccttagagg cggcatataa aggaaaacat acttgattaa ctaaatatag
    11761 atcttccatt tttccacctc cgctgtttta ctatagtgta tcatagcgga tttacacaaa
    11821 aaagtctatt cactaactgc tttcagagta acttctatat ttccagctgt agcttttgaa
    11881 tacggacaca ctttatgcgc tgcctctaat aattcttgcg tttcttccag agaaagacct
    11941 tcgatagatc cttcaatctc tacacctaag acaaagctcg tttcactttc tgaataaagg
    12001 gaaacttgtg cactgacagt agaagcccca gagatatttt tttgctgttt gatcaattct
    12061 aatgcactgt taaaacatgc actgaaacca gctgcaaaca attgttcagg gttggttgca
    12121 ttttcgacgc gtttaccagg agctgcaata ttcaattcga atgtacgatc gggattatga
    12181 acttctccag agcgtccgcc tgtgttgatc attttcgtag aatacatttt tttcataaaa
    12241 ttaccacctt taattatatt ttatacaatt gaattttaca caatataatt gtggaaagca
    12301 aagaaatgga ttggcataag ataaataaga gaaagcacat gaaacagacg cgctttctcg
    12361 aatagcaaac atatacaaaa aaatatcaga gacaaaaact aatctcatca taaacattac
    12421 atatatttct caaaaagttg ttttctttct tcaaaactta cttcacgatg gacctgatga
    12481 agcatccata aataaccaaa aggatctctg aacattgagt tagtcacacc aaagtccatc
    12541 atttctgtca tttcttgaat ctctacacag ccatttcgta atgctttatt atgtgtgtcc
    12601 tcgatatcag agacgatgat attgaaccag atcgtatttg gatgttcagc agtaggagca
    12661 gtcaaaccaa gagacgtgtt ttcatccagc atatggaatc tcgtgccata caaggtaaaa
    12721 atgacttcat tattgcctct agggtaatct gttgcctcaa tcagttgtat ttcatcaaag
    12781 attgtttgat acagttctag tgctttcaaa ctatcattta atacaaaatc tatttccgtt
    12841 cctttatgca taaagatctc ctctcctttt cagtcgaaca gttactagat tcttttatta
    12901 taccagttaa agaaaacgca ttcaaaaaaa tagcggattt acttaccaac tcattcgatt
    12961 ggatgataag taaatccgcg tcccattact tctgctacgc tgctgacaat gacaaaggca
    13021 cttggatcga tttcttctat caatggtttc attcgggaaa attcatgatc agtcacaacg
    13081 atcaataaaa cctcttttga ctttcgatta tacccgcctt ttgcctctag cagggtaagg
    13141 ccacgtccaa tctcagcact taagcgaagg cggatgtctt ccaaataatg ttcactcatg
    13201 accataacag atttctttcg attgatacct gtttcgatat aagtcatgac aattgaagtg
    13261 attgcaatag ataaaatagc ataaagaaac tcttctattc caaaaacaaa cagattaaga
    13321 gaaacaacga atgcgtctgt aaaaaataag ccaatcgaag tattcaagtg gaaatatttt
    13381 ttaaaaatga ggggagggat cgtcgtaccg ccgctagaag agttgttttt atataaaatc
    13441 gcaactccta aagcaaaaat cgcacttcca aaaatgacag acaacaaccg atctgaagta
    13501 atcattgttt ctggaactat tgcaatgatt aaaggaaaaa acaagcttcc gaaaagtact
    13561 ttaaaaaagg gttttttacc taaaaataga agagctagta ccaacataac gatattcaac
    13621 actaaaataa ctatagctcg gtcaaatcct aaaacagctt ccagtaatat tcctagaccg
    13681 ctgacacctc cagcagcaat atgatgaggc cctaaaaaca tattgatagc gactcctaat
    13741 atgaagaggg caaaaataat cttaatgtat gtccatataa attgtctcgt ttctttcatt
    13801 tatccacttc cttaattcat taataatact cttatcttat tataacttac ttttcacctt
    13861 aagttaactt taattaataa aaaaattagg ctagtctaaa ttattatgtt tcattcttct
    13921 agaatatact tctacgttaa aaactgttaa aaaactttta ctttagaaaa ttggagaaat
    13981 tgatcgctta agataaattc aggacactaa atagtacagg aagtagatta aaacttttaa
    14041 aagttttaag tctatctatt tggcaagttt aaaaaataaa aattcggtat caaaaaactg
    14101 ttaccgataa catttttgaa agcgttgtct tttgtttctc tatcacttat agtgatgacg
    14161 tcatattaaa acacaaggag gaaatcaatg gatacagctg caatttatca tcgaccggaa
    14221 agtgaatatg catttctata tacgaataca agatttcgta ttcggcttcg tactaggaaa
    14281 aatgatatca aaaaagtata tgtgctttgt ggagatccat ataccataac aactgaaaaa
    14341 tggtatcaaa aacaacgacc aatgaaaaaa tgtctaagta ccaatgttca tgattattgg
    14401 gaaattgaag tgacgagcga aacaaggcgc ttacagtatg cgttccatgt tgttggggaa
    14461 gataacacgg actgttttta cggtgatcaa ggaatttttc cttatcgaga aaatgtgatt
    14521 actgaaccaa atttttattt tcgaatacct tattttcatc aaatcgatcg attcactatt
    14581 ccagaatggg taaaagaaac agtatggtat caaatttttc ccgaaagatt tgcaaacggg
    14641 gacaaagcaa atgaccccga acacacttta ccttggggaa gtaaagatcc agaccgagaa
    14701 gacttcttcg ggggagattt gcaaggtgtg agggatcatt tggattactt gacggacctt
    14761 ggcgtcaatg gcatctattt ttgtcccata tttaaagcca catccaatca taaatacgat
    14821 acgattgatt attatgaggt ggatcccgct tttggcgata aaaaactttt aaaaaacttg
    14881 attgatgaag cacataaaag agggatacgt atcatgttcg atgctgtatt caatcatatg
    14941 ggtgtccatt caccacagtg gcaagacgtt ctcaaaaacg gagaaaaatc gatctataaa
    15001 gactggttcc acatccgttt tttcccagta gattcttatc agatgactga cttaccagaa
    15061 actgctgaaa atatcccata tgatactttt gcttttactc cattcatgcc gaaactaaat
    15121 acagcaaatc cagaagtgca aaaatatttg ctggatatcg caacttattg gataaaagaa
    15181 ttcgatattg atggatggcg tcttgatgtt gctaatgaag tcgatcatca tttttggaaa
    15241 aaattcagag aagcagttac agaaatcaaa ccagacatct atatccttgg agagatttgg
    15301 cattcctctc aagcttggct gcaaggggat gaattccatg cagtgatgaa ttatgcattc
    15361 acagattcga ttaaagatta ttttgcaaaa aagaaaatca cagcttcaca aatggtcagt
    15421 ggaatgaatc atcagcaaat gctttacaga gatcaggtga acgaaggaac attcaactta
    15481 ttagattctc atgatactgc caggatcttg accctttgcc aaggtaacaa agaattgatg
    15541 aagtctgtga tggcctttat gtttcttcaa aaagggtctc cttgtatcta ttacgggaca
    15601 gaaataggga tgactggtga agatgacccg gattgccgca aatgtatgat atgggagaag
    15661 gaggaacaag atctagagct attcggtttt ataaaagaac ttgtttctct aaggaaacag
    15721 ttgtctaaga ttatttctga aggttctact cagtggctaa tagtatatga tcgtgaagat
    15781 aagctctatt tcacacggga actagaggga cagatcatct atgtttactt taatcagtca
    15841 aaagaacctt gggtagtaga acaggaaaat gaggtgattc ttagtcaaaa ttgtcagttg
    15901 ttagaagacg ggaaagcaga gatacgacaa tacggatttt tgatcatgtg ttgaaaactg
    15961 ttaccggtaa caacaagaaa gcgtttgcaa atgaagattt gtgcccatta taataagggt
    16021 gtaacagcac gttgaataca caggaggaaa tgaagatgaa aaaaagaagt gtgaaacagt
    16081 tgggtttagg gttaatctct ttaggaatct taggcggatt agcggcttgt ggcaatggta
    16141 actcttcagc agaaggggaa gccgatgaag gaaaaacgtt aacagtttcc gttgattctg
    16201 gatataaaga ttatataaac gaaataaaaa acactttcga aaaagaaaac gatgtgaaaa
    16261 tcaagctagt agaaaaggac atgttcgatc agttggaatc attagcgtta gatgggcctg
    16321 ctggaaaggg acctgacgtt atgatggcag catatgaccg gatcggtgcg ttaggacagc
    16381 aaggacatct ggcggaagta aaactgggaa atgaagcagc ttacgatgaa acagacaaag
    16441 cccaagtgac ttatgatgga aaaatctacg gagaacctgc agtaatcgag acccttgttt
    16501 tgtactataa caaagattta gtcgatacag caccatcaac gtttaaagat ttggagaatc
    16561 tatccaaaga cagtcgtttt gcttttgagt cagaagcagg aaaaaataca ggatttttag
    16621 ctaaatggac tgacttttat tattcctatg gtttaattgc aggttacggt ggctatgtat
    16681 ttggtgatga tggcacaaat ccttcagata tcggtttgaa taatgcaggt gctgtagaag
    16741 gaatctctta tgcaacagat tggttcaaaa atgtttggcc aaagggaatg caagacataa
    16801 aaagtgcagg agacttcgcc agccagcaat tcatgtccaa taaaactgca gcaatcattg
    16861 acggtccatg gcaagcgcaa acatacaaag aaaacaatat caattatggt gtagccaaaa
    16921 ttcctactct aaataatgga cagccttatc aaccgtttgg cggaggaaaa ggatgggtag
    16981 tcagcaatta tgctaaaaac aaagacctct ctcaaaaatg gcttgattat gtaacgaatc
    17041 aggaaaatca agaaaaattc tttgaaatgg taaatgaaat ccctgcaaac caacaagcta
    17101 gggaaacagc aaaggctaaa aacgatgaat taacaacagc tgtgattgaa caatatgaaa
    17161 cagctcaagc gatgccaaac atcccagaaa tgggcgaagt ttggacaggt gcagaaaact
    17221 tgatgttcga tgcagcatca ggttcaaaaa caccaaaaga atccgcagat gaagcagtaa
    17281 aaacaatcag tgaagccata gaacaaaaat actgattaaa atgataaccg tccattttta
    17341 taaaaaatgg aacaggtgct tcagataaaa gtggcaagag cgagatggaa aattatcttg
    17401 ctcttgttgc cattatctta aaagcagttt gcggtttagc aagtttagag ggggcattca
    17461 gatgcagcaa gataaaacaa gcaatatcaa cgaaaaaaaa atcacgcttt gttcacttat
    17521 tccagggatg gggcagtttg taaatggcca agcttttaaa ggtatcgttt ttttcgccat
    17581 attcgtccta ttcattggtc agatgcttct gggcggattt catgcattgg taggattggt
    17641 tactttggga agtataccaa tggaagatca ttcattattt attttgattg aaggcacttt
    17701 gcaattcatc attacaatca ttttcttgat tttttacggt ttgaatcttt tagacgctta
    17761 tcgagtagcg aagatgcgtc aagagggcaa aaaaatcaat cggacgatga aggaagtcat
    17821 acataatata ggtgatgaag gttttcctta tctattgaca cttcccgctt atcttctcat
    17881 gatttttacg atcattttcc cagtcctagt tacattgttc actgcattta ctaattatga
    17941 cttcaatcat attccaccag ccagtttaat tgattgggta ggggctaaaa acttttttag
    18001 tatcttcttt ttaagctcat atcgtaatac tttttttgca gtcttttcgt ggaccgtgat
    18061 ttggacttta tgtgcaacaa ctttgcaaat cacccttggt attctcacag cggttgttgc
    18121 tcaccagaaa tttattaaat tcaaacgtat tttcggtatc attttcttat taccatgggc
    18181 ggtcccagct tttattacta tcatgagttt ttcgaatata ttcaacgata gcgtaggtgc
    18241 catcaatact caagtgattc ctttcataaa ccagcttcct tttgtagata tcagtgcaat
    18301 ctcttggaaa acggacccga actggacgaa agtagcaatt atcttagtgc agggctggtt
    18361 aggatttccc tatatctatg tgatggttac aggaatctta caatcgattt cagaagactt
    18421 atatgaagca gcaaaaatcg acggagcaaa tgcatggcag cgcttttcaa acatcacgct
    18481 tcctgctgtc ttcctagtgg cagcgccaac atttattaca cagtatacgg ggaatttcaa
    18541 taatttctcc atgatctatc tgtttaacaa tggtggtccc ggaagcgtag gcggaggagc
    18601 tggttctact gatattctta tttcctggat ctataaactt acgactggaa cttctccgca
    18661 gtattctatg gcttcagcaa tcacgttgat tatctccgtc atcgttatct gtgtgtcatt
    18721 gctggtattt aaaaagacaa atgcgttcaa tatgggggat taacaatgaa aaaatttcat
    18781 tcgagtgctc gattccaaaa acgactttct cactttttta cttacttttt tcttttaata
    18841 ctgtcagtca ttatcatcta tccgcttttg atcacagcaa gtactgcctt caagtcagga
    18901 aatattgcag cttttagttt agatctttca agtgactgga ctttaggaaa ctttactcgg
    18961 ctattcagtg aaacacttta cgggacttgg tataaaaata cattgatcat ttctgtccta
    19021 acaatgatcg tacaagtgac tgtagtaact ttggcgggct atacttatag tcgctatcgt
    19081 tttatcggcc gcaaatcaag tttgatgttc tttttagtga ttcagatggt accgactatg
    19141 gcagcattga ctgcctttta tgtcatggct ttcttattag aagcgctcga taagtactgg
    19201 ttcttgacaa tgatctatat aggcggtgga attccaatga atacttggct gatgaaagga
    19261 tactttgaca ctgtaccaat agatttagat gaatctgcta aacttgatgg ggcagggcat
    19321 ttcaggattt ttttccagat tgtattaccg cttgtcaaac cgatgctcgc cgttcaagca
    19381 ttatgggctt ttatgggacc ttttggggat ttcatgttag ctaagttcct gcttcgaaca
    19441 cctgaaaatc agactgttgc tgtcggctta caaacattta tttctgatgt gacaaaccaa
    19501 agagtctctc tttttgctgc tggggcaatt ttgattgcac taccgatttg tctattattc
    19561 ttctttttac aaaagaactt tgttaccggc ctgacatctg gcggaacaaa aggataggag
    19621 cagagtatca tgcaaacatt tccgttaaac tatttttccg cattacgtac gccaactcaa
    19681 gtgtttgcgg gacgcaaact attatcatgg cctaaatttt tcttgatatt tgtattttta
    19741 gtttccttga tggtcatgcc tgtgactctt ttttatgcca atcagatcca agcgattcca
    19801 ttagaacaat ttctatccgt tcattcgctg atcgatgaac aaggaaccca aaaattttct
    19861 gaacttgaat tatctgaaac tgggttacaa gcatcgcaac aaacgatagc tgtgacccct
    19921 gaaattttgg taggtgtgtc tttatctgag aaacaacaat ccgatcatgg aacttttatt
    19981 gattttgaga aggaacaatg ggtgatccaa caaaaggata aatctggtat acgccgctat
    20041 acgatgaatt atagcccatc tttccaacca gattctgtac gtacgcccga agatttccaa
    20101 cgatttttag aaagagaatt ttatgccagt aatcgtccaa caatcatttt atcttatagt
    20161 ttaagtcttg gtcttgtcct atttgtcatg acgagtctca ttttattcgg ggcttccttt
    20221 ttcttgtgga tgacaagaaa aagccaactt tcatccattc atacattcaa agagagtgca
    20281 aatttgatgc tgaatgttat gggaataggt tccatggcag cagcagttgt agggtttatc
    20341 cactttgact ttatcttgat gctaagtgtc caaacaacga taactgtctt gctgctttta
    20401 tgggtttttg tcaaaacaag attcaaagat aagcgtgtcg aatgacaagt ttaagcgatt
    20461 tatgcgacaa tgaatgtgaa aaaaatgaaa aataaagtag tttttgaaaa atttctgaaa
    20521 aaattaaact tcttagt
//
LOCUS       DASNNU010000050        19917 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.050, whole genome shotgun sequence.
ACCESSION   DASNNU010000050 DASNNU010000000
VERSION     DASNNU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.050"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..397)
                     /locus_tag="U8116_001675"
     CDS             complement(<1..397)
                     /locus_tag="U8116_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN8434672.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIV"
     gene            523..1464
                     /locus_tag="U8116_001676"
     CDS             523..1464
                     /locus_tag="U8116_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN8434673.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(1614..2510)
                     /locus_tag="U8116_001677"
     CDS             complement(1614..2510)
                     /locus_tag="U8116_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN8434674.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            2779..4119
                     /locus_tag="U8116_001678"
     CDS             2779..4119
                     /locus_tag="U8116_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN8434675.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            4337..4780
                     /locus_tag="U8116_001679"
     CDS             4337..4780
                     /locus_tag="U8116_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEN8434676.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(5284..6702)
                     /gene="nhaC"
                     /locus_tag="U8116_001680"
     CDS             complement(5284..6702)
                     /gene="nhaC"
                     /locus_tag="U8116_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN8434677.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(7105..8328)
                     /locus_tag="U8116_001681"
     CDS             complement(7105..8328)
                     /locus_tag="U8116_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEN8434678.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(8359..9198)
                     /locus_tag="U8116_001682"
     CDS             complement(8359..9198)
                     /locus_tag="U8116_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN8434679.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(9215..9895)
                     /locus_tag="U8116_001683"
     CDS             complement(9215..9895)
                     /locus_tag="U8116_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8434680.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(9897..10970)
                     /locus_tag="U8116_001684"
     CDS             complement(9897..10970)
                     /locus_tag="U8116_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN8434681.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(11026..11264)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(11307..11540)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11617..12288)
                     /locus_tag="U8116_001685"
     CDS             complement(11617..12288)
                     /locus_tag="U8116_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN8434682.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(12375..15053)
                     /gene="mgtA"
                     /locus_tag="U8116_001686"
     CDS             complement(12375..15053)
                     /gene="mgtA"
                     /locus_tag="U8116_001686"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEN8434683.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            15712..16149
                     /gene="ndk"
                     /locus_tag="U8116_001687"
     CDS             15712..16149
                     /gene="ndk"
                     /locus_tag="U8116_001687"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEN8434684.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(16171..17037)
                     /locus_tag="U8116_001688"
     CDS             complement(16171..17037)
                     /locus_tag="U8116_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN8434685.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(17197..19290)
                     /locus_tag="U8116_001689"
     CDS             complement(17197..19290)
                     /locus_tag="U8116_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN8434686.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         5816 a         4087 c         3528 g         6486 t
ORIGIN      
        1 tgacaataat ttgttctact tcttgaagca gtgcatcttt aataagttct cctaaggcat
       61 aactggaagc aagtcggata gtttgatccg ttggtttatc aagtagatct aaccccaatg
      121 cttgtgcaga ttcgatgact gcggtgtttt ttccttgcca agttgtcaat aaatagctga
      181 tttttcgctg acggaataag agatccatcc catcaatggt acgccatgtt cccctgagcg
      241 cgtaatagat tgcttctgca gtattttctc cgccatctgc aatcggcaca ttgatttttc
      301 ttcctttacg atcttcccag ccatttagaa ctgcctgatt tagttcaaat gaagaagcac
      361 ttcctttaaa tgaatcaatt gccgccaaaa tatccatctg tatccctcac tttcttctta
      421 agtaagacac tttttttcat ttttttcaaa caaaacaatg ctattttctt ccatttttct
      481 atatactaaa agggaactca ttgaataaag gagagaaaaa acatgaaaat cgcaatagct
      541 ggggccggag caatgggcag ccgaatcgga ttgatgctac atcaaagcgg aaatgaggtc
      601 ctcttgatcg atcgttggcc tgctcacatt gaagccattc gtacaaatgg gttgattgct
      661 gactttaacg gtaaagaagt ggtcgcaaaa ctgccaatct attcaccaga agaaatcatc
      721 gaaagcaacg aacacgtgga cttgatcgta gcattaacaa aagccaatca attagatgat
      781 atgttttgtt cgatccaatc cattattact gacaatactt atgttttgtg tttactaaat
      841 ggattaggtc atgaagatgt actggaaaaa tatgtgccaa agaaaaatat cttatttggg
      901 atcacgatgt ggacagctgg tctagctggt cccggaaaag tcacattgtt aggtgatgga
      961 gaaatcgaac tagagaactt ggaaccagaa ggcgaagcat ttacaaaaaa agttgtagaa
     1021 gtctttcaag aagcaaattt aaatccgatc tatagccata acgttcgtta ttccatttgg
     1081 cgaaaagctt gtgtaaatgg aacattgaat ggtttatgca cgattttgga ttgtaatatt
     1141 gctgaactag gcgcgcaaaa agcggcagag agcatggttc gaacaatcgt atctgaattt
     1201 gcttctatcg cagcaaaaga aggaatcata ctagatcaag aagaagtgta ccaacatatt
     1261 gcttctactt atgatcctga caacatcggg ttgcattacc catctatgta ccaagacctg
     1321 atcaaaaacc atcgtttgac tgaaatcgac tacatcaatg gtgccatttg gcgaaaagga
     1381 caaaaatatg atattgctac accatattgt gcctttttga cccaattagt ccatgcaaaa
     1441 gaaggaatcc ttggcgcaga ataaaaggct ctctgatgag aatcagctga atcagcagct
     1501 ggttctcttt ttttaagccc aataaaagat caaaaagaaa acacaactct catgatgcca
     1561 taagaattgt gcttgtttgt ttcagtacat aatctcttca aaatgtaggt tttttatttg
     1621 ttttcaacag aggagaggtc atattcgtat tgctcataaa tagtggaatc gttagtgata
     1681 aatcgttttg cccaattttc tgcatcataa aattccaata gctctgatag gattggctta
     1741 aatggtgtat gcagaaattc cggtaattca tctaaatcac gttctcgaag caaaaaaata
     1801 ttttcaggac gataaaaatg ataatgagca atcgcttgat tatccgtcac tagtttcttt
     1861 tgaaaataca tggattcaaa tgtccgcaaa gtcactcctt gctgaccctc ccgcaacagt
     1921 tccagaatcc cgcgcgattc ccctgttttt ttcaggtact cttcataagt aagatatcca
     1981 ggttgttcat tccctcgatg tggaagaatg aaatagttgg tcgtaacatc tctttttata
     2041 aattcctgct tatagctttc tgctcgttct tttctcccat tattcgttgc accaaaaaat
     2101 agatccgtct ttggttcgct agaagaaaaa ttcatatgtt tcgaataaaa tgaactatta
     2161 tgtttcagcc caagttcttt tgcttccaaa aaatcaaaat gataaatctc atcgattgcc
     2221 ggttcatctt gtgcctgctt cagcatcgcc tcgtgttctg ctttgaagtg attccaaaaa
     2281 aataacacaa tttttgcatt ggtaaatggg cgcacgcgtc gagccatctc catatcaaat
     2341 ccataatcta aaaaaacgat cagctgataa tttgccaact gttgtctagc tgatttaatg
     2401 aattccgact gtagtaaatc ttttccggac caaacatcta aagagcggat ggaaggcaaa
     2461 aaataaagtg atttattttt tgcaactagc agtacgtcat ttttgttcat cttttccgcc
     2521 tcactttctc tgtcactttt tcctatgata gcctgtttaa atagtaaaaa gccagtaaaa
     2581 cgtttcctat atttgtatga aaatttaaca tttgtatgaa aatatgatga aataaatcat
     2641 tcttctatta aaattcgcct aaaagttttc tgaatagttg taattgtctg aaaatttgtt
     2701 atcattagtc attgttaaat attgctattt attcttaata taaattttga ttgtgaattt
     2761 aaaagaaaga ggtcatttat ggaagataaa aaattgaaaa aacaacttag ctccagacat
     2821 atcaccatgt tagctttagg tggagcaatc ggtgcaggtc tatttaaagg aagtggcgaa
     2881 gcgattggga tcgccggccc ttctgtattg attgcttttt tgattggtgg gctgatttta
     2941 tatatcgtga tgaaaggtct gggaaaaatc gttttaagcg gtggcgacac gcatcacgga
     3001 ttatctggtt tgatacgccc ttatctaggt gctcattctg ctgattttac tgattgggtc
     3061 tactggtcta tgtggattat caatatcatc gctgaagctg ttgcagctgc ttcctttttg
     3121 cagttgtggt tcccgcacat tccagcctgg gtatttgttt ttatgttggc tgttttaact
     3181 acgatcatca atctttattc ggtacggtta ttcgcagaaa cagaatattg gctggctttt
     3241 gctaaaatca gtgtcattat cttactgatc atttttgcta cttatcttgt cggtcagcaa
     3301 atgttgggca caggcgtttt ccctaccctt cagcaattga ctgaccatgg tgggtttact
     3361 cctcatggta tgaagggaat catcagttct ttgcttgtag ttatctattc ttacggcggt
     3421 tctgaactga ttgctatcac ggtaagcgaa gcagatgatc ctaaacgtgc gattcctaaa
     3481 gcaatcaaag gtgtcatggg aagaatcatt tccttttaca tcattccttt gtttttgctt
     3541 ttgattcttt ttccttggaa tactttagca ggcacgaatg tcagtccttt tgtgatggta
     3601 tttgaaaaaa tgaatatccc atttgcagcg gatatcgtca attttgtgat tgtccttgct
     3661 ttgttctcat ctatcaattc gggtgtgtat gcatcttcaa gaattttgtt cttccgtttg
     3721 aaggatcgaa aaggttcctc tagaaaacta gctgttttaa acaagcatca agtaccgcaa
     3781 cgtgctgttt tattttgtac cagtgttctt tatcttggtg tagctctttc ttattttgtc
     3841 ggtgacaaat tatttggcta tctggcaggt tccctttcgt atacagtatt attgatctgg
     3901 atcattatca gtgcggcatc ctttgtactg gcactaagaa aaggaacatt atgggataaa
     3961 ggaatcagct tttttgcatt agctgtctta ggattgattt tccttggtat cctgttcacg
     4021 aactccattg gggtaactgt attgactggc ctgctttacc tgttcattta tttcagttat
     4081 caaaaaaaga atgatgcgtt cgtattgacg aatgaatgaa atcgatagaa aattttattt
     4141 tatttatgtg gaatagatgg agctattgaa gaaaggagtt gtgacaaaag tcttgttgca
     4201 actccttttt ccgaataaac agtctcatga cctcttggta aacggtgttc aaaaaccctc
     4261 tgtgcaaaat aattgattgt tctccatgct catgacatga tagaagaaag gaatgaaaaa
     4321 aaggagtttg ttttaaatga aaaagtattg gaagtttttg ttgatgatcg gtgtttcaac
     4381 ggtagttatg tttggtatga tgtacttgaa tgtctatgcg ctcgatcata tcttcttcag
     4441 tcgtacacga gtattcatgg cgttgatgat gggtgcagtc atggcagtca tcatgctgtt
     4501 gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgtt atgatcttag ccgctagtct
     4561 tcttgttttt gccggttcct tgtttatggt acgaagccaa actactgtca acgacacagc
     4621 ttggatgaag gccatgattc ctcatcattc tatcgctatc ctggcaagta aacgagctga
     4681 tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc attcaggcac aagaagaaga
     4741 gatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga ccattatccg tccaaataaa
     4801 aaaagaggat gttccagaag ttatcagctt caagaaataa gaagaagcta cttctggaac
     4861 gccgtgaata aggggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca
     4921 aaaatagctt ctcctatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga
     4981 acaccgttta ttaagaggtt gtaaaatcag cgttctgacc tgagtattag agagacaaat
     5041 ggaaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt cagctgattg
     5101 aacaccgttt atcgagaggt tgtgaaaaag tcgcttagtt tcgcgtatta agaaaaattt
     5161 tgtgaaatgg ctttccacat ttttgtataa atttggctta ataccgagaa actggctttt
     5221 gaataccgtt tattcgtaaa agggattgtg acaaaagtct tgtcacaatc cctttttatg
     5281 tttttattgt tctaattgcg ctgtcttgct tactgcttct tgcggtttca caaatttttt
     5341 tgagattccc caacctgtga aggcactgac catcgaaaac acaggagaaa gcaaactgaa
     5401 aaatacgaat ggcaagtaag tgagtgtagg aataccaaat gtacttgcta cgaaacttcc
     5461 ggcaattccc caaggcacta agtagttgat tactgttcca ccatcttcta gcgttctacc
     5521 taatacagtt ggatcaaatc ctgctttttc ataagtttct ttaaatgcat taccaggcag
     5581 aataacagat aggaattgtt caccaataaa aatatttact ccgatacttg tcatcaatgt
     5641 tgtaaaaatc aaatttggtg tgttcgtcat ctttcttgat actcgttcca tcacggttga
     5701 gatcaagcca aattcgatca acaatccacc taatgacaaa gttagaataa tcaatgacac
     5761 agtaggcatc atactttcga ttcctccgcg gctaagcagt gaatcgattt gttggtttcc
     5821 agtatttgaa acaaaccctg attcgatgac cccagccaac ttcgttaagg aagtttgtgg
     5881 gttttgaata aaaatcattc ctactgctac agcgatattc aacaaaatcg ttaaaattgc
     5941 cggcaccttt ttccatgcac acagtaacat taaaaataaa ggaatcagtg accagattga
     6001 aatagtaaag tttgcttcta gaacatcagt cacttcacga atagatgaaa gacttgcggc
     6061 tttacttgtg tgtcctaaaa tcatgaataa aaagaaagaa acaacaaatg ccgggacagt
     6121 tgaccacatc atatgcttga tgtgtttgaa caaatcggca tcaacaatag ctgctgctag
     6181 atttgttgat tcggataatg gagacatctt atctccaaaa acggcacctg aaacaatagc
     6241 tccaacaacc agcgctggat ttaagcctag tgttgtccca ataccgaaaa atgcaatccc
     6301 aatcgttgac ataacagtaa aggcactccc tacagcgcta cctacgattg cacacacaac
     6361 aaatacagaa gggacaaacc actggacact gatcaatttg aacccaatga ccattagagt
     6421 tggaatgatc cctgcttgta tccatacagc aatcaatgaa ccgactaaaa taaaaataaa
     6481 gattgggatg attcctggtt tgatgccatt gatgattcct tcgtgcagcc aatctgtcgg
     6541 gtatcttttt atcactgcat acaacatgac cattccgatc gctgctaaga tcgtgatatt
     6601 aggtgagacc tttaatccga caacgccaat cgcaatgcct aaaacaataa ccattaatac
     6661 aagtgttgct tctaaaaaac ttggtgttcc tttttttccc atgataatct ctcctttttt
     6721 agttgttctt atgttctaaa aaagttaaga tatcggatta ctccgccgca attcatccgc
     6781 gattcctcct ttataaaaat aaaaagacct gttttaaact aaggacgaat gactccgcgg
     6841 taccacctta attaaaaaca gatctgtttt tcactttcaa acttttattc aaatgtatgg
     6901 actttccatg taattcgtca attaggcttg atcggttcac agcgaccacc gactttctga
     6961 acaaaacacc taaccgctac tgcgtgaaaa gttgacttgt acgaagttta ccttccataa
     7021 gatcatttgt caatgaaaaa gaaaatattt tttcattttt ctgaaaatta tttttataac
     7081 tttcacacac aagattattt tttactagaa agtagcgccc tcttgttttt ttcttgctac
     7141 tatttcttga tgttcttgct tgattctttc taatagcttt ggctctttca acagatcaag
     7201 agctgtattg gcgagtagtt ctgctgcaat cgcaatagaa gcaaggcctt tgtcactttt
     7261 agcagcagct ttgaattctt ctgtgtgtcc ggcaatatat gtctcagaga ttgctaccgt
     7321 aggctggatt gttggtatga cttggctgac atttcctaca tcagaagaac cgagacttgt
     7381 tctttgttcc gtttctattt cttcatctgg aacacctgct tcttttgcat gctggaagaa
     7441 taagtcatca aaagaaggag taacgatgac atcatcaact gcattttgga acaatccaaa
     7501 ctcaaaagtc gtgcccgtac ttatcgcagc acctcgtacg atattttcca ctttttcata
     7561 tactttatct aatgtttgcc gatttcctgc acgtaaatag aaacgtccag cagcgtaatc
     7621 tggaatgacg ttggcagcta ctcctccgtc tgtaatgatc ccatggatat tgacatcttt
     7681 tggcaattgc aagcgtagag cgttgattcc attaaacact tggatcaatg cttccaaagc
     7741 atttactcct ttttcaggtg ctgctgctgc atgggaagcg acaccgtaaa atttgatgtc
     7801 gactggatca ttcgctaaag attctgtcgt tttcccataa cgaaaagctg gatggacgca
     7861 taacgctaca tcgacatcct caaaaaaacc ttctcgaaca aagctgcctt tagctgatcc
     7921 gttctcgccc ccttcttccc ctggggtgcc atatacacgg atttctccac cagtttcatc
     7981 aatgaattgc ttcactatgc tggcggctaa tacggagtaa gtaccaaaca aattatgccc
     8041 gcatgcatga ccaatacctg gtaaagcatc gaattctgcc aaaaaggcaa ttgtgggacc
     8101 gggtttattg gaaatataac gtgcatcaaa ggctgttcgg tgtccagcta cttctttttc
     8161 tacccaaaac ctttcttttt ctaattgttt aatcaacgct gctgaggaat aaaactcata
     8221 gttacttact tcaggatgtt cgtggatatc tagagcaagt gcttgatatt ctggtaaacg
     8281 atattggatt agatccttca attggtttgt tgagattgtt gttgtcataa aaaattcctc
     8341 ctatgctagt catttttctt attttctccc gaatgtttcc catgcaggca cgcttgatcc
     8401 ttttgatgtt ttctcgatta ctttgactgt gtcttctgtt tgataagcgt ctactatttt
     8461 ttgatacgtt ttattgtttt catcttcttt tcttgcaacg atgatattca cataaggacg
     8521 tgcatggtca tccactggtt ctaagaagat ggcatcagaa gcaggattaa atccagcatc
     8581 tacagctaca ccgctattga tcactgaaat gtctacatct gacaaagcac gtgctgtttg
     8641 ggaagcatct aattcagaaa tgtctagatt ttttctattc tcggtaatat cagaaacagt
     8701 gggcgcttgt ccttttgcct tatccacttt gatcaatcca gcagtttgca ataacagaag
     8761 agctctgccg ccatttgtaa catcattagg gattgctact tttgctccgt ctttgatatc
     8821 agctacatcc ttcaaagtgt tagaataaat tcccagcggt gcattgacag tatttccgat
     8881 cgatactaaa tcagttccgt gttctttatt gtaattgtct aaaaagaatt gatgttggaa
     8941 agaattcaaa tcaatcgatc catctgctaa agccacattt ggttgattgt aatctgtaaa
     9001 tttcacgtat tccaaattaa tgttctcctt tttcaaacgg tctttcacgt tgtcccatac
     9061 atctgtatct tctccaataa tccctagttt tacgatagtt tcttttttgt cagttgtatc
     9121 tgcttttcct tggctacacc ctgccaacca aataattgct acaaatgtaa tgactacaaa
     9181 acgaataatt tttttcattt tttctcctcc actttcaatg tgtaattttt ttaatcagtt
     9241 gattactgat aaattgactt aaaaaaacta agattagaat caataatgta gccacaatcg
     9301 ttacatctgt ttggaatcga ttataccctc tcgatatagc aagattccct aatccgcctg
     9361 cacctactgc tccagccatg gtagtcagtc cgatcacgtt gatgatagtc aacgccgaga
     9421 cccgaatgat accaggcaaa ccttctaaca aatacacacg taaaatgata ccgattggac
     9481 tggttcccat cgcttctgcc gcttcgatca ccccaggatc aacttctagc agtgcgttct
     9541 cgatttgacg agcaaaaaac ggaacaactc caaaaatcaa tggcactaaa gctgctttcg
     9601 caccaatcgt cgttccaact aagaaacgag tagtcaatgc taaaagagaa agcaagataa
     9661 tgaaagggat ggaacgaatg atattgatca gcttatcaag tatttcaaat agcagacggt
     9721 tagctagtaa tccattaggg cgtgtcacta ctaacgtcac acctaagatg attccaatca
     9781 tcccgccaat gaatgctgtc caaaaagaca tatatagtgt ttgcttcgtc gcttcgacaa
     9841 attcatccgg aatcaaataa acattgggca aatatgtttt aaagaaatca gtcattttat
     9901 cttcctcctt cgatcacttt gatacgacta tctaaatcgt ttttttgtaa aatatttatt
     9961 tttacaccag atttgatcaa ataatccaat gcttttttcc gctgtttggt ttctccagct
    10021 aaagtaacga tcaaactccc taacggcgtt tcttgtaata actctacatt gccatataaa
    10081 atattcgctg ttacttgata tttgctgtac agatgagcga ttagaggttc gttcgtctga
    10141 tttccaatat aacttaattc caccagccat ttattggcga tctgatcggc aaaagcagaa
    10201 tgggcaatga tcgtttctaa tgcttggtca agatgagtag ctgttcgaat aaaatctttg
    10261 gtcaattctt cttcaggatt gctgaagatc tggatgctgc tacctttttc aacgattctt
    10321 ccatcttcca taaccgctac cttgttgcag atttctttga caacttgcat ctcatgagtg
    10381 atcaggacga ttgttaaacc taactggcga ttcaattttt ttaacaatgc aaggatctga
    10441 agggtcgttt tcggatcaag tgcactggtt gcttcatcac ataataatac cttaggttca
    10501 ttcgccaaag ctcgtgcgat tgctactcgt tgtttttgtc cccccgacag ttgtttagga
    10561 taagcagatg ctttttcttc caaaccgaca agttccagta gttcattcac tttttgacga
    10621 cgttcctgtt tcgattttcc agaatacttt aaagaaaaat caacgttgtc aaaaatagtt
    10681 cggctttcca ttagattgaa atgctgaaag atcataccga tggcctttct ttcttttcgc
    10741 aattctttgg gagagagctg actcagattc gtctgattga caatgacttc tcctgctgtt
    10801 ggtttctgca gcaaattcat cacacgaact aacgtacttt tccctgcacc tgagtaaccg
    10861 acgattccat aaacatcgcc tttttcaatc gttaaatcta cagaatctac tgcctgtatc
    10921 tctttttctt tttgtttgaa acatacagaa acatctcgca attcaatcat taaaattcct
    10981 ccttatctaa aaaaagcgtc ctcaagaaaa tgaaaatgtc attttcttga ggacgctttt
    11041 ttgcgtgtta ccacctcatg ttcaagctgt gttcacacac aactcctctt caagtacgat
    11101 catactctag caagatatcg gtcgcagccg tcatcgcctt acatctatgc tcgggatgaa
    11161 ttgctcgatg gccattttcc aatagtcgtt ccttgcttct tctcagctac cgaagctctc
    11221 tgtcaaaggt tcttctatcg tacttaccat ttcaatgcaa ataatattca aaaaaaaagt
    11281 ccttttacta acataaacta tgtcagtaaa aggacgacgt atcgcggtac cacctttttt
    11341 cggaagtttc cttccctcaa gcccaacatc tcaaaaagag agattggcgt tcgttttaac
    11401 ggtcgaacag ttccgaattt gcctacttat cgacaaatta ttctccaagg ccattttcag
    11461 cttttcttac ttgactcttc tcaccagcca gagcctctct gcaaagtttc caaacttact
    11521 ttccttttca acgaaatcga tattttgttg ttgggattaa tctacatgag gaaaactgaa
    11581 aagtcaagta cgaaattcaa attatttttt ctatatttat ttttctgttt ttttatttaa
    11641 catttcaata acagttcgaa gatcttttcc ttccgcaaga agtttcaatt tttccttttc
    11701 ttcgacggta taatttcgga caaaactgga atcaggtatc aaaacaacgc ctaatcctgc
    11761 tcgtgaagca gctgttgctc ctactaaaga atcttccact gcaaaggctg aactagcttc
    11821 taatcctgtt cgttccaaag ctgtcagata aggaatcggt gaaggcttgt tctccgacac
    11881 ttcgtctccg aatgtaaacg tgtccaagta atcagctaat ttaaagtgat ccaagatatc
    11941 tgttgctctt ttcgtaacag tggtcgtagc aatgcctagt ttcatatgac gttcttttaa
    12001 gtctgccaat atttcttttg catatggttt taagtggatg gctccgcttg tcagctgttg
    12061 ataaaaatat tcttcacgct tcgctcggac ttcctgtact ttttcagggc tacctgctgc
    12121 tttcactaaa atatcaaaac tttgtttcca gttttttccg ccccaagcgt caacgatttc
    12181 ttttgggata tggatatcaa attgctgaaa tccccaaagc cagccatcac gatacgtttt
    12241 ttctgtgtca actaacaaac catctaaatc aaatattgca cctttcatct atacattaat
    12301 cctttccaaa acaatacaca tagtttagca cgactggtcc aaaaagaaca gaagcaaaga
    12361 accagccttc tcgtttataa gagttcttgg aatcgacgga tatagatttt cttcacagct
    12421 gtcacaagcg ccagataagc gatgactgta gcaaataacc ataaccagta attgccagga
    12481 agtggcatca agcctaattc agcaccaaaa cttgtaaaag gaaggacaga accgacaata
    12541 attcctaatg tagtgataac aaacatggca aatgttgcgt tgctttggat aaatggtact
    12601 ttcggtgtac gcagtgcatg gagcactaag gtttgtgtcc atagtgattc gacaaaccat
    12661 cctgcatgga ataatgcgat gaaaatcact cgctgtgaac tatccaatgt atggaaactt
    12721 ccgcccacta cagcaggaca aataacaaaa tacatcaaca agtaagtagt aatatcaaat
    12781 atcgaacttg ttggaccaaa gtaaaccatg aattccccaa tactagatgc attccatttt
    12841 tttggctcgt ccaaatattc tttatccatc cgatcccatg gcaaggaaat gcaggaaaca
    12901 tcatagatca gattcaaaaa caaaagttgt aaaggcagca ttggtaaaaa tggcaggaac
    12961 gtacttgcaa tcagcacgga aaacatattg ccaaaattag aacttgctgt agccttgata
    13021 tatttcatga tattcccaaa agtttctcgt ccagaaagaa ggcctctttc caaaatcgtc
    13081 aaatcttttt ctaaaaggat cacgtctgca gattctttcg caatatccac agctgtatct
    13141 acagaaatac cgatatcagc cactttcatt gcaggagcgt cattgatacc atctcctaaa
    13201 aatccgacag tatgaccatt tttacgtaaa gctgtcgtca aacgtgcttt ttgctgagga
    13261 ttcaatttta cgaatacttc atggtcttca gctacttgtg ctaattcatg atcactcatt
    13321 tccgagattt tctcaccggt aaccaattca ttgatctcta gacctacttg acggcacact
    13381 gaacgtgtaa ctaattcgtt gtccccagtc agaactttta cagctacacc atgttctttc
    13441 agagctttca acgcttgttt cgttgtttct tttggtggat caaggaaagc aagataacca
    13501 atcaaaacca tgtctgactc atctttgact gaaaattctc caacgacgct tggattggtc
    13561 ttttgtgcga caccaatcac tcgtaagcca tcttcattaa gtgactgtac ttttgcaagt
    13621 acggcttctt ttcgttcttt tgttaatggt gtaacagtac cgccaagatc aataaattta
    13681 gaaatgttca gcatttcttc aatcgcacct tttgtgatca tttgtgtttt accagctgga
    13741 tcagcaacca caacactcat acgacgacgt tcaaaatcaa aaggaatttc atccactttt
    13801 tgataattga tcttatctgt atctaaagtt tctgttgctt catcaatgat agccacatct
    13861 aacaaatttt tcagccctgt ttgatagtag ctatttagat acgcatggcg caaaacacga
    13921 tcatcttcat ttccttcaca atccatatga tattcaagaa tgattttatc ttgtgtcaat
    13981 gtccccgttt tatccgtaca taacacatcg attgcaccaa agttctggat ggcattcaaa
    14041 tttttgatca ctgttccttt tttggccatc acacttgctc ctttgaccaa attcgtcgta
    14101 acgatcattg gaagcatttc aggtgttaga ccaactgcta cagatagtcc aaaaagaaaa
    14161 gcttctagcc aatccccttt tgtaagccca ttgatgacga taactgctgg agccatcaat
    14221 gacataaatt tgatcagtag catggatgtt ttatggatgc ctaattcaaa gcttgattga
    14281 attggttttt cagatagcgt ttctgctaca tgaccaaata gtgtttcatt tccaacagaa
    14341 acaatgattc cttccgcact tccactgatg acattacttc ccataaagac aagattttcg
    14401 taacttgttt cgctttgctg atcgtcttca catactttcg catgcttttc tactggatag
    14461 ctttcccctg tcaatgctgc ttgggaaatg aataaatcct ttgcggacaa taaacgcata
    14521 tccgctggta tcatatcccc tgctgaaagc cgaacaagat ctccacagac gatttcttcc
    14581 ataggaatct ctgtgtactt tccattacgt tttacagctg ccgttacttt cacaagtgat
    14641 ttcagttttt cagccgcttg attggaacgt acggactgaa tcaacgtcat tgtcccgctg
    14701 acgataacca tagtgaagat aatggctaca ccgaaaagat ctttatctcc aggagctgct
    14761 aatacatatt ccgtgatgaa tgaaataatt cctaatgcga tcaacactaa tgtaaatggc
    14821 gtgatgtatg ctttcaatat ttctacaatg aatggtgttt ttttgccata tgaaatcgca
    14881 ttttcaccgt aattttctct tgaacgttcg atttgttctt ctgtcaatcc atttgctgat
    14941 gcatggaata gttcaaataa ttcttgtttc gtttttccag caaaagctgc atagattttt
    15001 tgtgtatctt tctttttttc catctcaatc tctcctttgt gtccaaaaga cagctgttct
    15061 cgctgctttt ggtctttagc tattcatcgg atcgatagag aaaaaactga aaggggctga
    15121 gaacagatag ataatagccc tggcctttcc tctagatcct tgtttttcga atgactgtca
    15181 tccatttgtt ctcatctcct ttactaagta attttaattc atgacttcca atcatttttc
    15241 gaatagttat ttttccaatt agaccacctc ctttttattt ttcaccgatg gggatttgcg
    15301 gcaacaaaaa aacctctcgc caaatactca gcgaaaggtt tgaaaaagca tattaaaaaa
    15361 agcttctcag atctcatcgt tgagttttga cactgtacaa cgtaaaaagc agatacctgc
    15421 ttttagctac cttatgaaaa gccttatctc gacaattcct gttctaccca ttggcgtctc
    15481 tcgacgtttt tgggcagtag cctatgtttg tataggagcc tcacctaaca aatcttattc
    15541 ggttttcctt cgttaatata acaactaaaa aagaggatgt caaatcaatt ttccatgttt
    15601 ttcatacttt ttttaaaata cacacgttat attcctgtct cacgcggttt tatttgtctc
    15661 attttccacc tcccgctata atagaaatat agtaggaaac gaggtgctgt tatggaaaga
    15721 acacttgtta ttatcaagcc agacggcgtg agacgccatt tagtcggaag catcatccaa
    15781 cgttttgaag caaagggact ggccattgct gaaatgaagt ttgaaactat gactcctgaa
    15841 ttagcaaaag aacattatca gcacttgact gagcgcagtt tttttgatga actgatcgat
    15901 tacatgactt ccggtcctgt cgtttatctt gtgctggtcg gcgaggaagt gatcgatatc
    15961 gtccgtaaga tggttggagc aacaaaagct gccgatgctg ttccaggtac gatccgagga
    16021 gactacgcgc tgccggggac tgaaaacatc attcacgctt ctgattcccg tgatgctgcg
    16081 gtaaaagaga tcgcccgctt tttcccgcca ttcgatataa acaaaccgtc tgaaaaagcg
    16141 gcgaagtgat acaaattcta aactgctggt ctagttgctg ggttcagcaa tggccagcag
    16201 ttttttctct aaggacctcc gttgattctt tcctaacata tattcgagca ctgtgaagaa
    16261 ttcttggtag tctttcgtct tttcttcccc taaagcagca gaaatcaatc tttgataata
    16321 atctgccatc aatgtttcat agatcactgt acttctttct ttatgtgcta agctccgttt
    16381 catatcttca ttcacactac gtgccttttt caaccgacca ttttctatat aaaacgaagt
    16441 caggttcatt aaaaaagtaa agagatactg ctcatgattg ttcaactgtg ctaatcgtcg
    16501 cattcttttt ttgattttca aataattgtt ctcaatgaac cgatcactga atatccaagg
    16561 aaaatcagct agaatagata gttcaaattg accatagtct ggtatctgca tcaagtattc
    16621 ttcaatgatt cggcattcat aacgtaaaaa ttcaaattct gtctcagata cttctgtttt
    16681 ttcgaccatg tgaagattca aaaccaaata atacagatct tctgttttct taaaatgcct
    16741 acggttattg ctgatctctt tataaggatc aaagctgtct tcttgtaccc gatctagcat
    16801 tcgtcttttt ttctttaact ttattggttc atccagctgg tttcgcatcc attcatattc
    16861 tgcaagtgtc atgttcattc gctctaaaaa tgatctcaat gttacaaatg acattttttt
    16921 cactgaattt gtctcaatcc cgactagaga agtcttaaaa cagattcctt ccgctaattg
    16981 ggactgtgtc aacttcttgt tgactcgaag tttcttgatc aattcaccat catacataat
    17041 agttctcctt tttaatttaa atttttccac attcaataaa tagatacgtt aaaaaggaaa
    17101 aatacacgaa aaaaataaaa aaatgttttt ttcataaaaa ataccaacct tcaagcatta
    17161 gaatgttgtc taactactga aggttggttt gattgtttat tgattatttt tttgctgttc
    17221 aacgtctgtt cttgttactg gatataagtt ggttgttgtt ttattaccat tttttgaaaa
    17281 gacacttgtc gaatgttcgc ctttttgctt ttcgattgct tcattacgtt ccgcttcatt
    17341 tttatagtca tattttttcg gatcaatcgt ctcaaatccg tttggcacat aaaaccgcaa
    17401 gagattttcc tgattcagct tatctgaaag acttaacgca gtcttgactt tttcctgatc
    17461 tttggagatt tcctcatcga ttcctgctgc tttcgtatcg atgatttctc ctgtctgatt
    17521 attgtaagct tttcctccta ggacggtgta tttgggtgtc acaaagttcc catttcgaaa
    17581 agcaactact tgatcatgct gagaagataa taagtctgta ccaaaatgaa gataatcttt
    17641 atcatcgatt cccaatagat gtagtaatgt cggcaaaaca tcgatttctc caccgtattc
    17701 atgattgact gtccctttag tataaccagg catatggatc atcaatggca ctcgttgcat
    17761 ctgtgcgttg tcaaattcat cccatgtatc tggatcttta ccaagagcgc ttgctaaatc
    17821 tttattatct gtattcgaga tcccaaaatg atccccgtaa atcacaaaga ttgaattttg
    17881 gtaaatacca gattttttca aatacgtaaa aaattgttcc aatgattgat ccagataatg
    17941 tgccgttcga acataattat ctactgtgct attgccagta ttcaaagatg gaaagtcgaa
    18001 attcttgtca tccgtataat aaggcgtgtg gttggttacg gaaagaaatt tcgtgtaaaa
    18061 cggctgttgt agatgctcaa gatattgggc agattctctg aataaatctt tatctaatat
    18121 cccataacct aacgtttcgt cggattcatc aaaataagat cgatcaaaga aattgtcata
    18181 acctaagttt ttatatacat gatctctatt ccaaaaacta gctacattcc catggaaaac
    18241 agcgcttgta tagccttgct tttgtccaag gatcgctggt gcggcttgaa agacattgtc
    18301 tgacccaagt tctgtgaata atgagccttg aggtaaacca aatgtgccag tttccaacat
    18361 attttcagca tcacttgtct ttccctgtcc tacttcatga aagaaattgt caaagctgat
    18421 cgttgcttga ttctggaaaa tactatttaa aaacggtgtg acttcttgtc cgtctacctt
    18481 catattaatc aaaaactgct ggaaactttc taaatggaga acgatcacat ttttcccttt
    18541 agcgatccca aatgtttcgg gatttggctc tgcataatgt ttctttgtat aatctaaaac
    18601 ttcatcaatc ccattactgc ttgcatgcgc gcgaacactg ctagtcattc ctgttttgat
    18661 tccgtcataa atagtatacg catcgattcc tagatatttg acaatatatg aacggtcaaa
    18721 tgttcgctgc agcaattgtg gacggttggc ttcgctcagc gctagatttc ctgaaaaaat
    18781 aagccctgaa agacaggtaa cagcgatagc catcggtttg ccgacagggt tgcttttgat
    18841 aggtattttc ttgaccttca accaaaccag tagcccaatg aacacagcga tatccagcca
    18901 gtagatgaca tcatggggct tcatcaaaga aaaggagctt cctgacaatc cttgcgacac
    18961 tttcgaaaag cctaatacag atttgactgt aatgaaatct gtgaattccc gataaaaaat
    19021 aatattcaag tacaacaaaa gcgtatttaa aatatcaatg atcaaaagaa tagctaacgc
    19081 tggctttggc tttttaatat acaaagaaag gccaaaaaac aataaggtag tcgcaattgg
    19141 gttgatccac aagataaagt actggatcgt accttccaca cctaatgaaa aatctgcata
    19201 gtaggcaatg atcgtcttca gccagaaaaa gaaaacagct gtcaaaacga ttgctaaacg
    19261 attttggcac aattttttaa gttgtgtcac gtatatcact tctctctatt cacatttttt
    19321 cttattttgc tcctgaaaaa tgaaaatttc ctaaaaaagt tgttatctta agattttttt
    19381 attctttcta aagattattc cgtccacttt tcacagcata aaaccgctgc gatgtaagcg
    19441 attaaactaa tgaagagata aaagtcttgt ggtgaccgct tcactccacg gtattcaaaa
    19501 gccagtttct cggtattcgt acaaaaaaag aggctgtgac aaaattcttg tcgcagctcc
    19561 ttatttcttg atgcaggaca gctttttcac aacttcttcc ttaaacggtg ttctcctgcc
    19621 aactcctcgg ccacaagtca caatatttat aaaacatgag aagctgtttt ttgaaattgt
    19681 tccttgtggc tcagagttaa acggcaggct caagacctct tgatacacgg tgttcaatca
    19741 gctgaccctc ggtattagat tatgtcagga cgctaatttt acaacctctt ggtaaacggt
    19801 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatata aggagctatt
    19861 ttttgaaatt gctccttgtg gctcagagtt aaacggcagg ctcaagacct cttgata
//
LOCUS       DASNNU010000051        19479 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.051, whole genome shotgun sequence.
ACCESSION   DASNNU010000051 DASNNU010000000
VERSION     DASNNU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.051"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            45..599
                     /locus_tag="U8116_001690"
     CDS             45..599
                     /locus_tag="U8116_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN8434687.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(772..1245)
                     /locus_tag="U8116_001691"
     CDS             complement(772..1245)
                     /locus_tag="U8116_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN8434688.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1257..1793
                     /locus_tag="U8116_001692"
     CDS             1257..1793
                     /locus_tag="U8116_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN8434689.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1833..2204
                     /locus_tag="U8116_001693"
     CDS             1833..2204
                     /locus_tag="U8116_001693"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN8434690.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2488..3759
                     /locus_tag="U8116_001694"
     CDS             2488..3759
                     /locus_tag="U8116_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN8434691.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4004..5029
                     /locus_tag="U8116_001695"
     CDS             4004..5029
                     /locus_tag="U8116_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN8434692.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5323..6432
                     /locus_tag="U8116_001696"
     CDS             5323..6432
                     /locus_tag="U8116_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN8434693.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6515..7411
                     /locus_tag="U8116_001697"
                     /pseudo
     CDS             6515..7411
                     /locus_tag="U8116_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7427..8251
                     /locus_tag="U8116_001698"
     CDS             7427..8251
                     /locus_tag="U8116_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN8434694.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8283..9593
                     /locus_tag="U8116_001699"
     CDS             8283..9593
                     /locus_tag="U8116_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEN8434695.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9741..11000
                     /locus_tag="U8116_001700"
     CDS             9741..11000
                     /locus_tag="U8116_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN8434696.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11010..11882
                     /locus_tag="U8116_001701"
     CDS             11010..11882
                     /locus_tag="U8116_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN8434697.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11894..12724
                     /locus_tag="U8116_001702"
     CDS             11894..12724
                     /locus_tag="U8116_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN8434698.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12764..14947
                     /locus_tag="U8116_001703"
     CDS             12764..14947
                     /locus_tag="U8116_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN8434699.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15008..16228
                     /locus_tag="U8116_001704"
     CDS             15008..16228
                     /locus_tag="U8116_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEN8434700.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16225..17685
                     /gene="gtfA"
                     /locus_tag="U8116_001705"
     CDS             16225..17685
                     /gene="gtfA"
                     /locus_tag="U8116_001705"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEN8434701.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17762..18868
                     /locus_tag="U8116_001706"
                     /pseudo
     CDS             17762..18868
                     /locus_tag="U8116_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19190..19399
                     /locus_tag="U8116_001707"
     CDS             19190..19399
                     /locus_tag="U8116_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN8434702.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
BASE COUNT         6637 a         2945 c         3804 g         6093 t
ORIGIN      
        1 tacataatca ttaactcaga atatttttaa gaagggaaac aatagtgagc tataatgtac
       61 aaccgttaag gacaaatgaa gagatacaag attttcagtt ttggctaaga agaacaagta
      121 atccggaaag agatagtttt ttatttttgt ttggaatcaa taatgggtta cgtatgtcag
      181 atattatagg attaaaagtt ggagatatac gaggcaaaag taagcctatt attgttgaac
      241 ataaaacagg taagcgaaaa ccgattttta tagataattt gagagaagaa attttattat
      301 atactgaagg taaagaagaa aatgattggt tatttccgag tcggcaacaa ggtcgacata
      361 taacaaggga tcgtgtctat cagatatatg cagatatagc agaaaaatta ggacgagatg
      421 atataggaac acatacatta agaaaaactt ttgggtatca ctactacaag aaaactagag
      481 acattgctac cttaatgttt atttttaatc atagctcgca agcaattaca aaaaggtaca
      541 ttggtataac tgaagatgaa ataggagcat cacttcgtgg atttaaacta ggtgtataat
      601 ttaaaaattt ttataaacag taaagacaaa gattaaaagc tatttttatc tagaacgatt
      661 ttatttcata aagttcgcgg aataaaagct ggggttactg gtacttggta taggttcgtg
      721 ttatataaac taagtaatta aataacattt ttaggttgga aaaggatgaa attaaaaaaa
      781 gagaatcaat acggtctcaa aaacgcaaac gctgcggaat aagaaaaata ctgataactc
      841 aacgtttagc agactatcta atagggatag tctgcttttt tcgtttttga ccatactttt
      901 gaccttctgc aaacttcctt gcttttcctc tgtagaactc atgaaatctg caaaacgaat
      961 tgccgtttcc tctgtctgct ttttcgcgat atgggtgtat atatcatcca tagtggtttt
     1021 aatatctgta tgtccaagtc gaacatacac ttctttgaca gacgcaccag attcaaagag
     1081 cagactacaa tgggtatgac gaaaaccatg aagtgtgata tgttttaagt tattctcttt
     1141 aatgatgatt ttcaaattgt gatttacata gtctaaataa aggggcttat tttcttctga
     1201 tgaaaacaaa agttgttcac catcttttat aggtttgatt ctcattttta gatattatgc
     1261 ccgcacgtat tcatcacatt tctattgtga atcgctttat tcgaccgaca tttgactttt
     1321 atcataatat actcggttta aaattactaa tgaaaacaat taatcaagac gatcatacca
     1381 tgtatcacct gtttttttca gataatcacc accgtgtggg aactgaactg actttttttg
     1441 aagtacaaga agggaacaac cagttttttg gaaccaatac gattgaacga acgatactaa
     1501 aagttccttc cgaagcatca ttgcattttt gggaaatata ttttgaaaca caaggcgttt
     1561 gccattatgg aatcgaatca ttttctggac gacctatctt acgttttgaa gggccagatg
     1621 caacacgatt agctttagtt cccttacgcg aatttgaaga tattgatgat tatttcccgt
     1681 atgtcacaga ccagatccct acagagcatg ccattttagg aattgatgcc attcaactac
     1741 gcgtacaata ttccgatgcg actgaacgag agttattgtc ggtaggctgg tagcacaaag
     1801 aatacgtcag tttcttttcg ggtcatcaac gagtgacaat cttagaaaat caagacactc
     1861 agttctatca agaagttcag attattcaag atcgtgagaa tccagttgcc attgaaggga
     1921 ttggtagcgt ccatcatgtt gcgtttggcg tagaaaataa atctgattta caacgcatcg
     1981 acaaacaatt acaagagcgc aattttataa attcaggaat aaaagatcgt gaattttttg
     2041 tatcacttta ttatcgtgac ccaaatcaat tattgattga aattgctaca ggggaaggaa
     2101 acctagatgc aaaagcgtat gaaaatcaaa gtccacgctt tgaagaaatt cctttatttt
     2161 taccgcagta cttaaatttc attcgtgaac aagtggagca ataattagct aatcaaatcc
     2221 atgacgaaga gtaagaggtt gactcataag tcaaaaaata aagaaaaacg catcaaatcc
     2281 attctaaaga atgttgattt gatgcggttc ctatttttat aaactatcat tttcgactaa
     2341 taaaactact tatgagtcag cctctctttt tttcttgatt tttatacctc tttctaggta
     2401 aactaaacat ctgccaaaaa taccgtgtct cattgaaaca ttctcaacta tttcaaaata
     2461 aaggggagta ccaaatgaac gagtatattg aaattatcca cgcaagagaa acaacttaaa
     2521 aatgtcagtc tcaaaattcc caaaaataaa attagtattt ttaccagcgt atctggttca
     2581 ggaaaatcat cgattgtttt tgatactatt acccaagaag ctggtcgtca actgaatgac
     2641 acctatgata gttttacccg tttattttta cctaaataca gtcgtccaga ggttgatgag
     2701 attgataatc tttctacggc gattgtggtc aatcaaaaga agattggtgg cagtgcgcgc
     2761 tcaacgttag ggacgattac taacatcaat gcgttatttc gtgtgttgtt ttcatgtttt
     2821 gcaacaccta gtttaggata tgcaaatgct tattcattta atgatcctag tggaatgtgt
     2881 tctgtctgtc agggaatcgg taagacgatt gcatttttaa taggattgct ttatttttcg
     2941 gctattgata atgctgattt tgtattttct gatccattta ccgcagaagt tttgtctgga
     3001 attgaggaac aggcaaagaa tcatggttat tttgtattgg tgcataatgt taattcagca
     3061 gaagatgtta aaactattca gagaaattgg cgttttgagg ggttcattgt tgttggtgta
     3121 cgtgtaaaag aatttttgga attaaataac gtttttgatg gtcctgttgt ttatattgat
     3181 acgcatcttt caaaagagac attaagagaa cttgaacaac aaatgaatcg tttatttgta
     3241 aatacaaatg attaccaagc tagtacattg gcagtggaac atttgattca ttatggtcat
     3301 gagaaaatag cgtttctttc ttttgactac attgtaggtg aaccaagtgt tattcaagaa
     3361 cgacatcaag cttatatcga tacattaaag aaatatcatt taacctttaa atcgcattgg
     3421 atatataaca actctgaatt tgagcgaatt tatcaagaat tagctgaatt tactgcaatt
     3481 gttgttactg gagattattt agcggctaaa ttagtaaaat ttttaaaaaa taagaataaa
     3541 cgagtgatgg aacaattatc gattattgga tttgatgata ttacttttgc agaattaatg
     3601 gatcctgcgt tgacaacaat acgactaaat ccaggtaaca agggggcgat tgcttttcac
     3661 caactgattg gactggccaa acaagaagaa ataaaaaatc aagtactact attagaggga
     3721 gaattgattt ctcgagactc tgttcacaaa atcaaataaa aaagctctgt gaaataagta
     3781 tagttaacaa taaaatatcc gaacgataga gagaatgaac tgtgatctag cagttcctcg
     3841 tcgcaaattc actagaatta gcattattta ctctaaattg ttcggatatt ttttcttgtt
     3901 tttaattatg attacagtgc ctatttttaa cgaaatttgc ttattttcaa aatgtatgat
     3961 tatatgttag taaacacttt caaatatgaa aagaggagct attatggtag gaattaggga
     4021 tgttgcaaaa cgggcgggtg tgtccattgc aacagtatcg cgcgtgctaa atgaggatcc
     4081 gacactttca gtaaccgatg aaaccaaacg aaagattgca gaatcggtta atttttatca
     4141 ttatgtcaaa aaaagtcccg ttgcaaaaaa tttaacaaat attgcattaa taacgacagt
     4201 ttctgaagta gatgagttgg aagatcccta ttttcgaacg attcgcagag ggattcaagt
     4261 cgaagcagaa caaagcaaag tcaatttgaa gaaaattatt cgtttgtcag aaacaccgtt
     4321 ggagcaagac gatttgctaa aatgtcaagg tgtgttgatt attgggcagg tgctatcttc
     4381 tgtgattgaa gaagtatcag caattaattc gaatgtcgta gtgattgatg accctagtat
     4441 tgaagctgaa gtagatgcag tttatactga tttaacaaaa gcaacgaatc agcatttaga
     4501 tcgattgtat aaaaaaggac accgaaacat tgcgtttatc ggaggaggac gtgttcgttt
     4561 agatagccaa ggaaataaac acgtgtcaga tgatgattat cgaaaaatag cctatgagac
     4621 gtggatgaaa caaaaaggat tagaaaaata tagtcaaatt tatttagaag gctggaccac
     4681 acttgacgga atgcaggcag cagatttact tttgaaaaaa catgctaaag ataagttgcc
     4741 gactgctatc atggtaggaa atgatcctat tgctgtgggg gtttatcgtt cacttcaaaa
     4801 aaatggtcta catattccag aagatatttc gattgtgagt tttgataata ttgaagttgc
     4861 tgaattttta acacctgcat taaatacggt gaatgtgaat acagaagaaa ttggaaaact
     4921 agcagttcgc atggttaatg aacgaataaa agaagttaga aatgtttcga tcaaagtgat
     4981 tgtttctaat aatattattg ttagagaaag tgaaaaaaat atcgattaaa aaagtaaaca
     5041 tccaaaaaag ggtgtttact tttttgttta ctaatatttt tagtaaaaaa tctcgacaaa
     5101 acattaagta aacagttatg gtgttgttaa caaaaagaaa acgcattcaa aacaggagga
     5161 attaagatga aacatttcaa aaaaatagct ttaacaggga tagctgtttt aactgggaca
     5221 ttactcgcag catgtggtgg gggagatagt aattctaaag acgggcaagt aactttaagc
     5281 tatgcgagtt gagatactga ccaagctcca ggactaagaa aaatgttaga tgaatttgaa
     5341 aaacaaaatc ctgatatcaa agtagaaatg gagacaacgc cttgggatca atattgggtt
     5401 aaattagaag ctgcaacaac gggaggtaat atgccagatg tagtaaccat gcactctagc
     5461 gaatcctata aatatatgtc acagaatgct ttgatgggac ttgatgatgt tatcaaagaa
     5521 aatgatattg acatgggaaa ctttactgaa ggaattgctg atttctatac gcttgaagac
     5581 gaactatacg caatccctaa agatgcttct gtagtggggc tatggtacaa caaagaatta
     5641 tttgataatg caggtgtcga ttatccagat gaaacatgga catgggaaac actcagacaa
     5701 gctgcaattg atttaacaga tgaatcaaaa ggcatctatg gttttgcatc acatattgga
     5761 acagagactg gctactggcc atttatttat caaaatgggg gagaagtatt tgccgatgac
     5821 aatacaaaat caggtttgaa taccccagaa gcacaagaag ctttagcttt ttatcgagat
     5881 attgcacttg ttgatcatgc ttcaccaaca attacagaat tacaagatcc tgataagtta
     5941 agtcgttttc aatcaggtag attagcaatg atgattgaag gaaactggtt tgcaccggga
     6001 tttttgaaaa atgactatct agttgaaaat gttgcttctg ccgtgttacc taagggaaaa
     6061 gttaatgcaa caattacaaa tggacttggt tgggctgcca gtgcgagtac aaaataccca
     6121 gaagaagtga aaaaactaat ggcattttta gctagtgagg acgccaataa aatccaagcg
     6181 gaaacggggg catcaatccc agcactaaaa ggctttgaag acgagtgggc tgcttcttca
     6241 gatacaattg atttgagtgc ttttggaaaa atgttggagt atggagtccc tcgtccatac
     6301 aataaagatg gattacgagc tgaacaagca gaagttcaaa ttattaaccg tattctgtct
     6361 ggtgaagtaa ctgttgaaga tggaaccgct caaattgaaa aagaagtcaa tgcgatattg
     6421 agcggaaatt aaattagtta attatacaat atacaggcga aaggattcta tttcgtctgt
     6481 atattaatgg atgaatacca aaaaggggga tattatgaaa aatcgaattt ttggaaaata
     6541 tcgtaaagat caagtgatat ggggctatat ttttattttt ccaacggcat taggattaat
     6601 tttattaaat atatggccag caattcaaac agcaatttta agttttcaaa aaacggtagg
     6661 gttcggtgat actgaatggg tcggattaag aaattaccaa aaaatgtttg cagacggtga
     6721 agtttttcag tctttattaa acacattaat ttatgcagtg gtttcgatac ctagtattgt
     6781 tgtattatcc ttgttggtgg cagttttaat gaatagaaaa gttaaaggat tgtcgattta
     6841 tcggacaatt tattttctac cagtcgttgc tgcaccatcc gcagtcgcaa tgatttggcg
     6901 ctggatgttc aataatgatt atggactgat taataacatg ttggctaaaa taggtctcga
     6961 tggaccagca tggttaacgg atccatcgat tgcgatttat tctattatca ttgtaggagt
     7021 ttggagtgct attggttata acatggtgtt attactagcc ggcttataag aaattcctaa
     7081 agattattat gaagcagcgg aaattgatgg tgctagtcca atccagcagt ttttcaacat
     7141 tacgttacca ttagttacac caacattatt ttttgtagtt gtaacaacag ttatcaatgc
     7201 tttccaagta tttgatgtta tttttatgat gattactcct aatagtaccg ccatgtttaa
     7261 atcacaatca ttagcctatt tattctataa acattcattt attttaaatg acaagggata
     7321 tggttcggca atcgttatgt tccttttagt gattatatta attattactg caattcaaat
     7381 gaagttgcag aaaaagtggg taaactatta aggaggctat caaattatga aaaataaatt
     7441 tttttcaaat gcgccaatcc atatcatgct tattttaggt ggtattgcaa tggcattacc
     7501 ttttatttgg atgattttaa cttcagggaa aacgttggtt gaatctaccc aaattccacc
     7561 tacaattttc cctgagaaat ttcagctaag taactatcaa caagtacttg atttattacc
     7621 attcatggct ttttatatca atacattttt aatggttgtt ggacgagtga ttggctcggt
     7681 tttattttca gcaatggctg catatgcttg tgcgcgtcta agttttccag ggaaaaatat
     7741 ccttttcagc ttagtactta ttcaaatgat gattcccggt tccattttta ttactccaca
     7801 gtatttatta gcacaaaaat tgggtatttt aaatacaatt ccagcattaa tattaccagg
     7861 ggtagttagt gcgtttggta cgtttttatt aagacaattt ttcttaggtt tacctgatga
     7921 attggaggaa gcggcctatt tagaaggtgc caatatttgg acgatctttt tcaaaattat
     7981 gttgccgctt gctaaatcag gattggttgc acttggaata ttcacagcgt tgtttgcatt
     8041 taaagattta atgtggccat tgattgtcaa tacttcaatt gataagatgc ctttagctcc
     8101 gggattagca aacttacaag gacaatatgc cacgaacttc ccacaattaa tggcaggttc
     8161 gttattagca atttggccaa tagttgtgat attcatcgtt ttccaaagaa aattcattga
     8221 aggaattgct acaacaggta gtaaattata aaactattaa ttagtgattg gagagtaagt
     8281 acatgacaaa aattacattt attggtgcag gaagtactat ttttgcaaaa aacgtattag
     8341 gcgatgcgat gttaactcca agtctacaag atgcacatat tgcattgtac gatattgatg
     8401 aaaaacgact aaaagaatca aatttaatgc ttaaaaatat taataaaaat tcgaatgaaa
     8461 atcgtgctga tattgtttct tattatgatc gcaaagaagc gttaagagag gctgattttg
     8521 ttatcaatgc tattcaagtg ggtggttata aaccttcaac agtgattgat tttgatattc
     8581 ctaaaaaata tgggttgcga caaactattg gtgacaccat ggggattggt gggattttta
     8641 gaaccctacg gacactacca gtaatgttag actttgctaa agaaatggaa gaagtttgtc
     8701 caaatgcttg gttattgaac tatacaaatc cgatggcgat gttaaccatg ggtgttttaa
     8761 aagcgacgag tattaagact gttggtttat gtcatagcgt tcaagtatgc gtaccagaat
     8821 tgtttgagca tttaggaata gccgatcaat atgacttaaa tgactttcaa tggaaaattg
     8881 caggcattaa tcatatggca ttcttgttgg aaattacaaa aaatggggaa gatttctatc
     8941 caacaattcg tcagttagct gcagaaaaac caaatccaca tccagattct gtacgttttg
     9001 aattgttgaa acgttttggt tactatatta cagaatcaag tgaacacaat gcagaatacc
     9061 atccatactt tattaaatca aaatacccag aattgattga cgaattaggg attccgttgg
     9121 atgaatactt acgtcgttgt gaacaacaaa ttaatgattg ggaaagcatg cgagaggaca
     9181 ttgtaaataa tggggagtta acacatacga gaagccgtga atatgcttca tatatcatgg
     9241 attctattac gacaggaact ccgtcagtga ttgcaggaaa tgtcttaaat aaaggcttaa
     9301 tcacaaactt accgacagat gtttgtgtgg aagtaccttg tttagtcgat aaaaatggcg
     9361 tgcaaccaac ctatgtcggt gaattgccaa cacaattagc tgctttaaat cggacgaata
     9421 tcaatgttca agaactgaca gtagaagccg ctttaacact agataaatca aaaatttatc
     9481 aagcagcgta tttagatcca catctatcat ctgaattgtc tttaacagat atcaaaaatt
     9541 tagtggatga tttaatcgaa gcacatgggg actatttacc tgaatataat taaaaagaag
     9601 ctgaaagagt aaggaatttt caattcctta ctcttttctt agttaaattt agtaaaaata
     9661 ttgacaaaac acttagtaaa cagttacatt agtgttaaca aaaagaaaac gcattcaaat
     9721 tgtataagga ggagcataaa atgaagtggt ctaaaaaaat tattttagct tgtatagcac
     9781 taggagcaac gatctctttg actgcttgtg gaaaaaatca ggaagatgca agcggtaagg
     9841 tagaaataga gttttttaat caaaaaaaag aaatgactca aacgattcag gagattgcta
     9901 aagattttga agcaaaaaac cctgatattc atgtaaaagt tgtggatgta ccgaatgctg
     9961 gtgaagtaat taaaacacgt atgcttgcag gcgatgtacc agatgtcatt aatctttatc
    10021 ctcaaagtat agaattgaaa gaatgggcga aagctggtta tttagaggac ttaaccgaag
    10081 aaccgtattt agagaatatc aaaaatggat atgcacaacg ttttgctatt gaggatcgtg
    10141 tatatagcat accattaacc gctaatgtct atggttttta ctataacaaa actgcttttg
    10201 aggaaatggg gattcaagca ccagaaactt gggcagaatt tgaaaaaata gtagctgaga
    10261 ttaaagatca aaacaaagta ccgtttgcga ttgcaggagc agaaggctgg actttaaatg
    10321 gttatcacca actagctata gcgactgctg ctggtggtga agaggaagcg aacaatgttt
    10381 ggcgtttctc agaagttaat ggaattaatg ctgaaagcaa agaaatgcaa aaagatttta
    10441 atcgattaga tttgttaaga gaaccaaaag ctttgcaaaa caactggcaa ggtgccggtt
    10501 ataacgatac agtagttact tttacaaaag gtgatgcgtt gattatgccg aatggttcat
    10561 gggcaatgcc gatgattcat tcacaaaatc cagattttga agtaggaact tttccttttc
    10621 cagcagatga agcaggtcaa agtctaacga ttggcgcagg agacctagcc ttatcagttt
    10681 ctgcaacttc tcaacataaa gaagaagcca aacgttttgt agaatatatg acaacacctg
    10741 aagcgatgca gaaatattat gatgtagatg gtgcaccgtg tgctgttgag ggcgttatcg
    10801 agaatactca ggattcacca ttaagtggtt taactgaact tgcttttact gatcgtcatt
    10861 tagtttggtt agccaaagat tggaatagtg aaaatgattt ttatacctta acaacaaatt
    10921 atttacacaa tggcaatcaa aaagccatga ttaacgcatt aaatgctttc tttaatccaa
    10981 tgaaagctga cggaaggtag gaaaataaaa tgaaaataaa agaaatttta aatcgttact
    11041 gggcaatcat ttttgtagca attccaatta ttttgcagat gatttttttc tactttccaa
    11101 tggttcaagg tgctttttac agtttaacta attggactgg tctgacttat aattttgatt
    11161 ttgtgggttt gaacaattat aagattcttt tttctgatcc gaagtttatc aaatcgattg
    11221 gcttcacttt aattgtgacg ctttgcatga ttgctggaca aattggtttt ggtattttga
    11281 ttgcgcgggc gttgaacgca aatattaaag ggaaaacatt ctttagagca tggttctttt
    11341 ttccagcggt cttatcgggc gtggttgttt ctttagtatt taaacaaatt tttaactatg
    11401 gtttaccaaa aattggtagt atgttaggca ttgaattttt acaaaccagt cttttaggaa
    11461 caacatgggg agcgattttt gcagtaattt ttgttttact ttggcaagga attgccatgc
    11521 cagtcatcat tttcttagcg ggttttcaaa gtattccaca agaaattatt gaagctgcta
    11581 gaatggatgg ggcgaatgat aagcaaattt tttggaatat tgaaatcacc tacttattac
    11641 caagtatttc aatggtattt attatggcat taaaaggtgg cttaacagca tttgatcaaa
    11701 tttttgcttt aactggtggt ggaccaagcg atgccacaac atctttgggg ttacttgttt
    11761 ataattatgc ttttaaaaac aatcagtttg gttatgcgaa tgctattgct ttagtcttat
    11821 ttatcttaat tgggattgtt tcactggcac aattaaaatt atcgaaaaaa tttgaaatct
    11881 aggaggtttc ccaatgaaaa catcaaaaga aaaaaatatt tctaaatact tgcttttatt
    11941 attagggggc ttcatgattt tagttccctt agtttcaacg ctattcggtt catttaaaac
    12001 aacacccgat attatgaata atttctttcg ttttcctaat ccagcgactt tggaaaattt
    12061 ccgaagattg ttagcggatg gaattggtca ttacttttgg aattcggcaa tcatcacaat
    12121 cattgcagta gttgctgtga cagcaattat tcctatggct gcttattcac tagcaagaaa
    12181 aatgactcgt gggaaagcat acggattgat gtatttattt ttaattttag gaatttttgt
    12241 tcctttccaa gtaattatga ttccgattac cgttatgatg agtaaaattg gtttagcaaa
    12301 tatgtgggga ttaatcttac tctatctcac ttatgccgta ccacagacac tatttttata
    12361 tgttggtttt attaaactaa atgtgcctat cagtctggat gaggctgctg aaattgatgg
    12421 ggctagtcac tttacaactt atttcaaaat tgttttccca atgttaaaac caatgcatgc
    12481 gacgactatt attcttaatg ctctatggtt ttggaatgat ttcatgctac cattattaat
    12541 tttaaatcgt gattctaata tgtggacact accattgttt caatataatt atacgggaca
    12601 atattttaac gactatggtc caagttttgc ttcctataca gtaggaattg tcacgattac
    12661 actagtttat cttgctttcc aaagaagtat tatagctgga atgagcaatg gagcagtgaa
    12721 ataaccaata aattattctc attataaagg agttcataaa cttatgatta tatttgacaa
    12781 tcacacacaa acatttcatt tacaaaataa aaaaattagc tatctattag gaatcgaaga
    12841 aaataattat ttaacgcatc tttattttgg aaaaaagatt acgcactatt ctggtgggta
    12901 tcgctacccc cgaacagatc gttccttctc acccaacaat gcaaatgcgc aagatcgttt
    12961 attatcttta gacacattac cattagaatt tccaggatat ggacatggtg attttagaga
    13021 accggcgcat aatattaaat taggaaacgg tagtcgtgta aatgattttc gttatgatag
    13081 ctacgaaatt caaaaaggaa agcccagctt agcgggattg ccagccttat atacggaaga
    13141 tgatgatgaa gctgaaacgc tgattattca attgaaagac gcgattagtg gattgacttt
    13201 acagttgttc tacacaattt atcaaaatca cagtgtaatt attcgttcta cgcaattagt
    13261 aaataatagt gatgaaacag tggagattaa ccgcttagct agtcaatcaa ttgattttcc
    13321 aaatcgtcca ctcgagttaa ttcatttaaa tggtacctgg gcaagagaac gtcaaatgac
    13381 tagagaaaaa attcatacag ggataaaagt attagatagt aaacgcggat caagcagcca
    13441 tcaacaaaat ccgtttgttg ctttagtaga tgccaaaaca gatgaatttc aaggagaggc
    13501 atatggattt aatttagttt atagcggtaa tcacgaaacg gtgattcaaa aagatccatt
    13561 tgaccaaaca cgggtcatta tgggaattaa ttcatttaat tttggttggc aactaaaaac
    13621 tggcgactca tttcaaacgc cagaagtcgt gatggtttat agtaatgaag gtctgaatac
    13681 gttatcacag acgtatcatg aattgtataa tcaccgttta attcgtggaa actatcgttt
    13741 aaaagaacgt cccacgttaa tcaacaactg ggaagcaacg tattttgatt ttaacgaaga
    13801 aaaaatcatg aatattgtcg atgatgcgaa gaacttgggc attgaaatgt ttgttttaga
    13861 tgatggttgg tttggcggtc gtgaaaatga cttcacttca ttaggtgatt gggttgaaac
    13921 agaaggcaaa cttgcttgtg gtttggaaaa tttagccaag caagtgcatg ataacgggat
    13981 gaaatttggg atttggtttg aaccggaaat gatttctgaa gatagtgaac tatttcgagc
    14041 acatccagat tgggcattgg gtattccagg tcgtgaccgc tcaattagtc gaaatcaata
    14101 tgttcttgat ttttcaagac aagatgttcg tgataatatc tatcaacaaa tgacggcgat
    14161 ttttgatcgt gtaccgattg attatgtgaa gtgggatatg aatcgaaata tgacagaagt
    14221 ctattctgtc ttgttggact caacgatgca aggtgaagtg gctcatcgtt atatgttagg
    14281 gttatatgaa tttttagaaa agttaacaac gaattatcca actattttat ttgaaagttg
    14341 ttcgggtgga ggcggtcgtt ttgatgcagg tttcttgtat tacatgccac aaacttggac
    14401 gagcgacaat accgatgccg tggctcgtct gaaaattcaa tatggaacca gcttagtgta
    14461 tccaatttca agtatgggtg cgcatgtttc agcagtccca aatcatcaaa caggaagaga
    14521 aacgagttta actattcgtg gcaatgctgc gatggctggt gtttttggtt atgagctaga
    14581 tctaacgcaa ttaacttctg aagaaaaagc agaaattaaa gaacaagttg ccttttataa
    14641 aaagcatcgc caacttttgc aatatggaac tttttatcgc ttggaaagtc cgtttgaagg
    14701 caatgatact gcatggatgt ttgtctcaaa agaccaaaca gaagccatgg tcttttattt
    14761 ccgagtatta gcagaagctg cgcatccatt agtcacattg aaattagcag gattagcaga
    14821 agaagcaact tatcaaatgg aagatgtaac atttgctggc gatgaattga tgaatctcgg
    14881 tttttatatt gactcagaac tacatggtga ttatgcgaca caacgctttt atttgaaaca
    14941 gatatagtag cttgaattta caatataagt gcaacaccaa aagagactca ttccaaacga
    15001 agaaaatatg ttttatttac acgcatttac taaaaagcag cctgatttga attgggaaaa
    15061 tgaagaagta cgtgaagaaa tctatgcaat gattaactat tggttagata aaggactagg
    15121 cggatttaga atcgatgcga tattaaacat caaaaaaaaa ctagagtatg gcatctttga
    15181 atctgatgga gaagatgggt tagccttcat tgggccctgg attcttaatc agtcgggcat
    15241 tgaagtgtgg ttaaaagaaa tgagagagcg gacatttaaa ccacataata gtatgacggt
    15301 agcagaagcc gatgttccta atgaacgttt agatgaatat attggtgaag atggctatta
    15361 ttcgatggtt tttgatttta gttatacgga tattgatgta cctgaaactg gtgaatggtt
    15421 taaagatagc caatggacat ggacggatat gcgtaccaat atttttacga atcaattagt
    15481 tacgcaagat aaaggttggg gcgccttata tttagaaaac cacgatcaac cacgttcaat
    15541 taataaatat attccagaag aatggatcaa tgattatagt aaaaaaatgt tggcgacgct
    15601 ttttatgtta ctaagaggta ccccttttat ttatcaagga caagaactag gaatgaccaa
    15661 catagaaatg gcatcattag aagattttga tgatgtagct acgcattctc agtataaacg
    15721 ggcattagaa tatggattgg caccagagca agcattgaaa gctgtcaata aacgaagtcg
    15781 ggataattct cgcacaccta tgcaatggaa tacgcaaaaa aatgccagat tctcaacttc
    15841 tgataatgta tggttaaaag taaatcctaa ttatccagaa ctcaatgcag aagctcagtt
    15901 aaccaatcaa cagtcggtat tcaattatta tcatcaatta attaaattaa gaaaaaatag
    15961 tgtatacaaa gacgtgctaa tctacggaca atttctacca gtgaaagata aaaatgaaca
    16021 tattttgctt tatgaacgcc aattagaaga taaaatcgta ttagttatct taaacgttac
    16081 agcaacagaa caagaatatt atgtagatga acagtaccaa caagtattaa tcaataatta
    16141 tgaagatgtt ttattagatc aaagtcgaaa attgcgttta cgaccgtttg aaagtattgt
    16201 attagctaat tatggaggac aattatgaaa attaaaaacg aagcaatgct catcacctat
    16261 gcagatagtt taggaaaaaa catgggcgaa ttaaaacaag tcttagacaa acatttacaa
    16321 ggtgttattg gtggtgtaca tctgttgcct ttttttccat cgacaggtga tcgaggcttt
    16381 gcgccgagtg attatacgac agttgatcca gctcttggaa catgggaaga tgtggaagca
    16441 ttaggtgaag attattattt gatgtttgat tttatgatta atcacatttc tcgtgaatca
    16501 aaatttttcc aagatttcaa aaaaaaccat gagcagtcac catacaaaga aatgtttatc
    16561 cgtattcatg aatttttccc agaaaatcga ccaacacaag aagatattga tttaatctac
    16621 aagcgtaaag acaaagcgcc gtttcaaacg gtagaatttg cggatggtac gacagaagag
    16681 gtttggaata cctttgggga agaacaaatt gatttagatg tgaccaaaga agttgtgaaa
    16741 gaatttattc gagaaaccat caaagatatg gcgaaccatg gctgttcttt aattcgatta
    16801 gatgcgtttg cttatgcgat taaaaaatta gataccaatg atttcttcgt ggaaccagat
    16861 atttgggaac ttttagacga agtgcgtcaa gaagcagcga aatatgacgt cgaattatta
    16921 ccagaaatcc atgagcatta ttccattcaa atgaaaattg ccaatcatga ttattatatc
    16981 tatgacttcg ccttgccaat ggtcactctg tattcacttt atagtggaaa atcagaacgt
    17041 ttagccaatt ggttgaaaat gagtccgatg aagcagttta caacattgga tacgcatgat
    17101 ggaattggtg ttgtggacgc tcgtgattta ttaacagatg aagaattgga ttacacctct
    17161 gaggaattgt ataaagtcgg ggcgaatgtg aagaaggtct attcaagtgc caattacaat
    17221 aatttagata tttatcaaat taacagcacc tattatagtg cattaggcga taatgaccgt
    17281 agctatctgt tagcacgtgc gattcaagta tttgcgccag ggattccaca aatttattac
    17341 gttgggttat tagcaggaaa aaatgatatt gaactgttgg aagcaacgaa agaagggcgt
    17401 aatattaatc gtcattacta tgatttagcc gaaattgaag aagaagtgca acggccagtg
    17461 attcaacaat tatttgcact tttaaaattt agaaatgaat ctgcggcgtt tgatttggat
    17521 ggaacgattg aagtagcaac gccatcagaa tcagaaatag tgattacacg taaaaataaa
    17581 aacggtagtg atagtgcaac attaacggct aacttggata cgaaagaatt tatcattgag
    17641 tctaaagata taaaaatttt cactagtgtt gcattaaaat tttaacctat tccatagaac
    17701 aaaaaatcct ttatactagc acttggatga cttatccaag accaataaaa aggactcata
    17761 catggataag tataaaacaa atacagcatt taataaatgg ttttctgcca taaaattagg
    17821 aaaactttct gtcaactccc aaaaatgtat ttttgattac aattcgtatc gtaaaaaact
    17881 gtcttttgaa gctgtgctca agctctttct ctacgcagtt aatgaagaaa aagaaagttt
    17941 gcgtgatttg agtacagctc ttatcgaccg atcgctccaa ctagaaaccg atgtgtcatc
    18001 gattagtcat acccaattgt ctcgagcatt taatgcactt gaacctaaag tgttagaaga
    18061 aatttttcaa caactgttag aaaaagttca acatcaaatc aaaccaacca agcgaaatca
    18121 tctgtatctg attgattcca gtacgttttc gcttagttta aaacgtcata agtgggcgac
    18181 tttccgtcaa acaaaatcag gtgtcaagtt acacttaaac tattgttata tggacgatac
    18241 tacgatgtat ccaactgatt ttaccttaac aaatgcccat gaacatgatc tcaatcaact
    18301 accagtattg gtgaatcaac cagaggcaac ttacgtgttt gatcgtgggt attaggactt
    18361 tgaaaagatg gatcagatgc attgggatgg ctatttcttc gtcactcgga ttaaaaaaaa
    18421 caccaaagtt cacgtgatag atactctgga aacatcgcca gaaacagaaa ttctacgaga
    18481 cgagctggtt cgtttaggat ctaaaaccta tctaaccgcc aatttccgac tagtcactgt
    18541 tcaagataag aacggacgtg tgtttcaatt tatcaccaat cgaatggacg tctcttcaaa
    18601 agaaatctct gatatgtatc acgctcgttg gcaaatcgaa ttgtttttca agcacatcaa
    18661 acaacacatg accattaaaa ccttcttttc tcaaagtgaa aaaggcgtgc agaatcaatt
    18721 gattttaacg atgatttccg ctctcctcac gtttctcatc aaattggaga cgaaaacgga
    18781 aaaatcagtg ttccaaatca aacgattctt tcgttattta ctgtttcagc cctttgaatg
    18841 ttgtctagaa aaattaattc ccacatgaga aaaaagaaat ttggctcgtt acttttggtt
    18901 ttcagttgaa taacagataa aaaattttct ggaaaaagtc atcctctgcg tcagcacagt
    18961 tttttttgac ttattttcag aggaaatagt tttcagaata ttctaaaatg ttttatgcaa
    19021 cactagtgat attttttaga attagatttc taatgtgcat tttttgagta ttaaggagca
    19081 agtatacaaa gatttataaa gctgattaaa tttgaataaa aatatgcagg aacgctacta
    19141 tttttagtag tgttcttttt tatatttgaa taaaaaatgt ggtggttaaa tggtggttat
    19201 aacaaaaaat gaattaatga aattaggtta tgggaagtat cagtctgaga gaatcatacg
    19261 acaggcaaaa gcaattatgg tttcaaaaag ctatacctat tacgaaaata gaagattagg
    19321 actagtacct gttgaatctg tagaagaagt tttaggtatg aagatagagg tagaggagga
    19381 ttttcagaat catggttaat tttaaaaaag ataaaaatgg gaaatatcat tttactgcta
    19441 gtttgggatt cgacaaacga acagggaaaa gagtacaaa
//
LOCUS       DASNNU010000052        18517 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.052, whole genome shotgun sequence.
ACCESSION   DASNNU010000052 DASNNU010000000
VERSION     DASNNU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.052"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(15..356)
                     /locus_tag="U8116_001708"
     CDS             complement(15..356)
                     /locus_tag="U8116_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434703.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            complement(368..583)
                     /locus_tag="U8116_001709"
     CDS             complement(368..583)
                     /locus_tag="U8116_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434704.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            complement(606..1496)
                     /locus_tag="U8116_001710"
     CDS             complement(606..1496)
                     /locus_tag="U8116_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HEN8434705.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            complement(1511..2134)
                     /locus_tag="U8116_001711"
     CDS             complement(1511..2134)
                     /locus_tag="U8116_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEN8434706.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            complement(2261..2578)
                     /locus_tag="U8116_001712"
     CDS             complement(2261..2578)
                     /locus_tag="U8116_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434707.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            complement(2580..3632)
                     /locus_tag="U8116_001713"
     CDS             complement(2580..3632)
                     /locus_tag="U8116_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEN8434708.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            complement(3550..5079)
                     /locus_tag="U8116_001714"
     CDS             complement(3550..5079)
                     /locus_tag="U8116_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN8434709.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            complement(5091..6506)
                     /locus_tag="U8116_001715"
     CDS             complement(5091..6506)
                     /locus_tag="U8116_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN8434710.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            complement(6499..7332)
                     /locus_tag="U8116_001716"
     CDS             complement(6499..7332)
                     /locus_tag="U8116_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HEN8434711.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            complement(7491..7721)
                     /locus_tag="U8116_001717"
     CDS             complement(7491..7721)
                     /locus_tag="U8116_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434712.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            complement(8468..8935)
                     /locus_tag="U8116_001718"
     CDS             complement(8468..8935)
                     /locus_tag="U8116_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEN8434713.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(9010..9450)
                     /locus_tag="U8116_001719"
     CDS             complement(9010..9450)
                     /locus_tag="U8116_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN8434714.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            complement(9480..9773)
                     /locus_tag="U8116_001720"
     CDS             complement(9480..9773)
                     /locus_tag="U8116_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434715.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            complement(9804..9998)
                     /locus_tag="U8116_001721"
     CDS             complement(9804..9998)
                     /locus_tag="U8116_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434716.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            complement(10104..10556)
                     /locus_tag="U8116_001722"
     CDS             complement(10104..10556)
                     /locus_tag="U8116_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434717.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            complement(10582..11043)
                     /locus_tag="U8116_001723"
     CDS             complement(10582..11043)
                     /locus_tag="U8116_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN8434718.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            complement(11244..12095)
                     /locus_tag="U8116_001724"
     CDS             complement(11244..12095)
                     /locus_tag="U8116_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN8434719.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            complement(12110..12910)
                     /locus_tag="U8116_001725"
     CDS             complement(12110..12910)
                     /locus_tag="U8116_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN8434720.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            complement(12953..13843)
                     /locus_tag="U8116_001726"
     CDS             complement(12953..13843)
                     /locus_tag="U8116_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEN8434721.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            complement(13845..14786)
                     /locus_tag="U8116_001727"
     CDS             complement(13845..14786)
                     /locus_tag="U8116_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEN8434722.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            complement(15019..15354)
                     /locus_tag="U8116_001728"
     CDS             complement(15019..15354)
                     /locus_tag="U8116_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434723.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            complement(15510..15683)
                     /locus_tag="U8116_001729"
     CDS             complement(15510..15683)
                     /locus_tag="U8116_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434724.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            complement(15680..15868)
                     /locus_tag="U8116_001730"
     CDS             complement(15680..15868)
                     /locus_tag="U8116_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434725.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            15946..16170
                     /locus_tag="U8116_001731"
     CDS             15946..16170
                     /locus_tag="U8116_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN8434726.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            complement(16167..16322)
                     /locus_tag="U8116_001732"
     CDS             complement(16167..16322)
                     /locus_tag="U8116_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434727.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(16462..16674)
                     /locus_tag="U8116_001733"
     CDS             complement(16462..16674)
                     /locus_tag="U8116_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434728.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(16677..16889)
                     /locus_tag="U8116_001734"
     CDS             complement(16677..16889)
                     /locus_tag="U8116_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8434729.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            17164..17574
                     /locus_tag="U8116_001735"
     CDS             17164..17574
                     /locus_tag="U8116_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8434730.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            17587..18039
                     /locus_tag="U8116_001736"
     CDS             17587..18039
                     /locus_tag="U8116_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN8434731.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            18130..>18517
                     /locus_tag="U8116_001737"
     CDS             18130..>18517
                     /locus_tag="U8116_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEN8434732.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIKNKLSLLKLKEKEYIIDD"
BASE COUNT         5318 a         3767 c         2783 g         6649 t
ORIGIN      
        1 atctgcttca ttcattaaaa aaactccaat cctccacaac caataactct tcgtatcaaa
       61 tcaccatagc ctagaagtaa ctcactaaca tcatttgttg ttgaagcata actaatcgtt
      121 gtatcgccac gtctaactga agatactgtt ttttctgatt cattttttat tattttatac
      181 aacacttcac taatcacgct ttttaacttt tcccatgaaa tatcattttt acaagtgttg
      241 tatgattcaa tttctagcaa aattatttct aataaattag ttatgcgttc ttcactcaaa
      301 tctggaaaat cttcttttgt agactcaatg atttccgttt ttagcgattc atccatcaga
      361 tcacttccta aatttcatta acatcaattg catcttccaa aattgcaatt gcttctttat
      421 catctataga aatgataaat tcgttatttg aatctgctgt gataaatcta cgagttttag
      481 gatgaacaaa cccaacaaaa tttttagttt tacctactct atatttcacg acttcatctt
      541 ttttagtttc atcttttttt accactgtaa ttcctcctat caaaaaaggg agtactaact
      601 cccttttatt cagactttaa atttaaaatt gccccagagt ttgaagcatt gtattcaaga
      661 gagtattcac ctacaagacc gatacgtcta gaatccgttg tttttgcaag ttcttctgct
      721 ctccattcac gtaatggacg tagtttcaca taattagtat cgattgcggc aattgttcct
      781 tttggcaatg atggttctag tagagcaata cctgtaccgt aattagacac gatatttcca
      841 atttgcaatc caaaagttac tctttccccg aactgaacaa tttttgttga ttttccatct
      901 aactcatcag tcattaactc ctgcatatct ggagaaatca agcatagttt ttctcccata
      961 taaccttttt catacatcaa tttaaataaa gtatcgatat cttttcttgt tactgcaccc
     1021 gcagtggctg tttctgcctt atttgctgaa ttgatcaagt tcaaaatccc attcattcga
     1081 cgacctttag aaccattttc atcagctttt acaccagtaa tcaattttcg gttcaaatca
     1141 attttcattt ccataccacg tagtgctact tgattagtta attcatttcc gactccattc
     1201 acattaatag catctaatgt accagataca gaggttgatt ttctaaaaat ttcagtatag
     1261 ttgttaaacc aagttcgacc tgattcagca tctggatatt ctccgccctc aagtttttca
     1321 gaagaatcat cgttgttgat atcatattca cgccatttaa tttctgtcga gtttgctggc
     1381 tctgtttttc cagcaccaag taaatagctt aaaaaaggtg tatttggtac ttgcatagca
     1441 ttaatcgctg gtgaaatatc caaatactct aaattattta aagatgtttt tttcataatt
     1501 tttcactctc ctagttaaat tgttgaagaa tttgtcctaa ttgttcttct ggatttgtta
     1561 atgtttcatt gttctgctga acctggttat tcgattgttg agttccacca aatgcagact
     1621 tcatttgtag ttcttttaat gcatctgcat gtttctcatt aattgcattc aaaacacttg
     1681 taaaaccttc aacagcattc ttagtgaaat ctgtatccga actaacaaga ttatttagca
     1741 taaattgaga aatagactct tttaactctc catctagttc taatccagca atttgttctg
     1801 caacaaatgc tttattatca cttgtcacac gcaacgcttt ttctgcttca aattcagctt
     1861 gtagtttttc cagttgaatt tgctcaggtg acttgttttt cttagattcc tcatattcct
     1921 tgatggtatc ctgtttaatt ttatctaagt tattttgttt ccaagcttcc aattgtttgt
     1981 cggcaacact ttgagattgt gactgaataa atttttgagc ttcttcattg gattccacaa
     2041 aagatttgaa gtcatcaaaa gaaaactttt gatcgtcacc ttcggcaaag tattgcaaat
     2101 tcattggcat taatgatttg tgtttcatgc tcattctcct ttcgccccac gattcgtttt
     2161 cacgccccgc attgctttga gttttaatag ttgcgcccca ccattcaacc aagcccagta
     2221 ttgcgctagt ttaacgtcat ttcggacaaa ataaaaaagc ctaattacta gactttcctt
     2281 ctttgtagtc agttctaaca acaatacatc ccatcttttc ataccaatca actgtttctt
     2341 ttaaatttgg taatgaatga gataataatt gaatcgttaa attaacggta ttcttatcaa
     2401 ctggagtatt atcttgataa ttaacagttt gatcgccaat aaccacgtag gcataattgc
     2461 cattccaatg gtctctcaag ccattaggat ggttctcaga ctcaatagct gttttgtagg
     2521 cttctgcaat atcagcattt acattaattg ttagtactgc ttcaaaaaaa tctttcattc
     2581 tacttcctcc gtttcaaatt aattccaaaa gaatcatgtg caaactcatc taataaatcg
     2641 ccaaataaac ggtcatattc ttcatcaata tcctttccta attttggtaa attaggaaca
     2701 tccgtacatc tacaacgact atggaaaggt gctcgatttt cacctatgac agcatctttc
     2761 agtttatagg gattcttact tgcctttcct ccacaaatcc ggcaaactct ctcatctttt
     2821 gcagtcaata cgttgtattc ctcaatacct gtttctaaat atgatttttc aataccatct
     2881 tgtgcaaatt ttgcatactc cgttcgaacg agattttcta tcgctttgtt atactttgat
     2941 tcttctaact taaacatgtc acaaatttct gagtctaatc tcatggtgtt taatgcgtgt
     3001 ataacgcctt ctccactagc aacacttttt acaatagcat tagagagttt ctgttcaagc
     3061 gatgaaatat ttccccaaag tcttcttgaa aatgtctttc ctgaccatgg atagttcaaa
     3121 taatgtttaa tttcattttc tggtaaataa tttggtatat caacgtttaa aagttgaaca
     3181 aaaacattag cattagaaga atatgttctt tgaagaattt cctctaagcg atcagaaaaa
     3241 tattgattca catctgcatc catcgcatat tctgcaagaa ttttaaatac atctgatcgt
     3301 atttgtaata agcgattgac ttttgcatag tcaaatggtg gaaaaaattc atcgataaac
     3361 tctttgtatt ttgcatcata catttttaat gacttataat ttttttccac atattcacga
     3421 tattttttct gattacttgt actgtaaaac tccatcattt ctggataagt gatattgtgt
     3481 aagtcagctt gtgataatag ttttgcttgt atctctttca gtgcttcagg aaatacacta
     3541 gttaattttt taagtgtctg attttcttgt ttgagccttg cttgatcctc cagttctcga
     3601 cgtttggtcc aatacttcgt ttctatcgtc actattgcca cctccgccaa aattgtattc
     3661 actatctgga taagcacttc cactttccaa ttccatcatt tcattttcat aatcaacatc
     3721 tgttacaaat ggaatttggc tctgaatagt tcgtttggat acgtatggtg caagtttagg
     3781 taaagcttca gctaaataac ttaaatcagt tggcaaagat ctagagaaag taaaaataat
     3841 tttttcaggc tcaatttcta ctttgtcatg aaacttaaga aaagcagcaa ttgtttctgc
     3901 gcattctttc aatccttctc taaaatattg ctctttagta tttgttttcg cttctaggct
     3961 aatgatttgc cacttgcgag cttctccaga actgtttgat ttaaacacct catcgttaaa
     4021 atcgattgct ttacaaatgg tgtaaaactg ttttttcaat agatccatat gatattcatt
     4081 gaaatctttt gctaaatttt ttgtgacata ttcagctttt gcagtagggg cttttaagtt
     4141 gataatccct agctgattca tcatttcttt agcagtgcct ttgctcattg ttgtgccagt
     4201 aacaagcatg tacgcaagct taaattgttc gatttcgttt tgttgatcag acaaagcacg
     4261 atcaattgca tctccaattt cctcagctac ttcaaaatca caataacgat tcgtattatt
     4321 tttaaattct gccaaggtaa tcgtccccaa agggttatcg gtctcatcaa tttttttgaa
     4381 cgttccgctt gtaacaaaat tcaaatcacc atagcaagca tatgtcagaa ttttatttct
     4441 tgtcataact ttcatttctt gaaaaaattt tttctggtaa gagtcatact tttctttgat
     4501 atagattccg gcatttccat atctttctgc tctccaaggt tcaatattac ttgctcttaa
     4561 cttccaacca tcatcttctt ccacaggctc tagcaatcgg aaagctactc cacaagctcc
     4621 ttgaaaagtt gcagtttcag aatcaagcat tgcgaatctc atggaatcaa gattgcttgt
     4681 taatttgtta aaagcttctg gaactgtcgg aagttctata tcatttttta agaatttatc
     4741 ttttaatctt tgaatgagtg ttcttttttg ttccgataca tcgtaatccc atttgatagg
     4801 tataccagta aaatgattga ctgcttggtc tacgacaatt gaatacatac cagcatgaag
     4861 tttattatta acttttacaa tatcagtgtt aggctttggt ctattatcta tttcattctt
     4921 ttcgctagta taagctagat attttctttc gcgatcagca aaaaatggtt tcatatcttc
     4981 tataaaacca ttcggatcaa acattccatc ttcaatttgt gttgcatatt taacacgtag
     5041 cttcttatat ctctctaaag ataatactgt actaatcaaa agctcacctc ctaaaattga
     5101 ataaattgat agtgacttgg tggctcataa aacgctaaag ccagtgcgtc agcaatatcg
     5161 ggactaccaa tatttcgctt tttcatatca tctttacttt ctaaacgtat acgacttcta
     5221 cttgtcattt tgaatttgcg agtacttaat tctttgatta acgaactatc agaaggcaat
     5281 tcaataacag gctgttctcc gttaagattt gctgtcatat tttcttctaa catttccttg
     5341 atgtttcccc atagttgagt acctaaatta tcgtaaaaat cgtcttctga tgttgaaccg
     5401 ttattcactc caaacacctc aaaaggataa tgtttgtctt ctataagttc ttctaggcgg
     5461 tcggttacac cacctccgac accagtgtca tcgactttaa tcatcacttt atcaatactc
     5521 ggatattgac tcattagatt cttggccatg ttgatgacat atcctgttgt ttccatggtg
     5581 ctacgttttg aatacttctc atactccaat gccctggtag caattctagg aaagagaatc
     5641 gtagaatcat caccatatcg agctacgtcg acaccaatat gagcaacctt tgttttattg
     5701 actaaagaat cactaatttg tttttctgta gccaattcaa ctgtttcaag actgataaat
     5761 gaatccaacg cacctttcgg aaattctcca aaaatacgga cacgagcaac atcactttct
     5821 tttccatatt ttttaagaat catttctata ttgtctttgt ttgtacgttt actatcatag
     5881 cttgatactt tatgaactct gtatttatca cggtctgaat tatgggaatc ataaaagaca
     5941 ccttcaatat tattggggtt tccacacatc aacagcttat tatcaaaacc tgatagcgta
     6001 ccaagaatag cttccataat tggatcagac acaccagaag cttcatctac cacaatcaac
     6061 atatggtcct cgtgaaaacc ttgcatactt tctggtttag tcgctgttct agccgtagca
     6121 aaccaacgct ctgaatcacc aaccatataa attttggtct ttgtccactt cagtaagttt
     6181 ttgatcaagc tatcatttaa ccatttagct acctctgccc aaagtacatc gtaaagttgc
     6241 ttcattgtcg gagctgttgc tattacttta gcgtagggtc gacacgttaa gaaccaaagt
     6301 attgctcctg cttctaatgc tgtttttcca actccttgac cagaacgaac agaaaccttt
     6361 gaaaattcag ctaaatcatt taaaacattt tcttgccatt catcaggatt caaatgcaaa
     6421 atatcttggc aaaaagctac tggtttatca tagtaataat caatggcaga accaatatca
     6481 gcaaaaggaa taaattcatt attcattttc tttcaccgcc cgtttgtttg cggcatttaa
     6541 aactgcctgt ttccattctt ctatttcttc acctgaatta tttccgccaa tttcatttgt
     6601 ctcagcttgg atcttatcag tctgagcttt aattaaatca gttctgtatt cattcatata
     6661 aagttcattc atttgcttga tggctttagc taactggttg ctaatccgag tcaatgaatc
     6721 ttcaattgaa agaatatcat caattttgcg atgttttttt ctgctaatct gcacatcttg
     6781 cataacctca cgcttgattt ctagcttttt accatttttt tcaatcggag ttttaatctt
     6841 tcttagctgc tgcaatcgtt cgacttcttc atcatttagt ccagcttcag cttgttggat
     6901 tcttttcatc attcgaagtt gtcttatttt aagaagtcgt atttcttcgg acaaagcaaa
     6961 agaaggatca tcattcaaac tagaatagat gtccttctca ttatcggtta acgtgtcaaa
     7021 aaatattgtt tcatactcac ctgttttcaa agcgttttta ttacccactg gcggagaagc
     7081 tcggctattt cctttgttac ctttagcatt ttgattacca ataggagcgc caccttgatt
     7141 agtaacgtta cttttgccat tggtaacgtt actaatcaat tcagcgctcc atttgtcttg
     7201 cgatttccat tttctaacct gagaatctga aacatttaat tcagatgcaa tttcctttaa
     7261 ctgcttttct ccgttggatt ctaaccaaat tcttttggct tcatcacgtc ttggatcacg
     7321 ttgtcttgcc attcaataca caccacctca cattctgttt aggttgagtt ttgttttcta
     7381 attttcaata tcttttagca ttaaatcagc ttcaatcaat atctttaaat cggaaacttt
     7441 gtctaacttg atttgtcctg attggagatt tttaagccat ttcttgcatt ctaatccctc
     7501 aaattcttgt taatattatt ttgaatgttt ggttcatcat agaaaccatg accgcaataa
     7561 acgagcttgc acgcatcaat ctcacatggt tctgcttctc tccccatttc gacaatagag
     7621 tacttccctt tgatttgtac agaacgcaca acacgcactg aacaatcatc aatggttcga
     7681 ggatattcat tagttagcga tacataccag tagtttctca ttatgtagcc tcctttatgt
     7741 aaagaaaaag accactcaaa gagtgatcta atatgtaatg cacaggcagg gacgtttccg
     7801 atcctgtgct tgagacattt gacgattcat ttgtaccgaa tcccaaatct caatctaacc
     7861 taaccagtta tgtaatagca acctacacga tgcacaaaac gcgtacgaaa ttgcgcaccc
     7921 ctatattttt aaaccgccga tgcctcggtt gccaaagtca ctggcaagga atcgaacctt
     7981 gcatggttgc cgaagcattg acctagcaca catgcttagc gtctaccctt tccgccacag
     8041 tgacactata aaattattct tggctgctac tattttttat tttgcccatt tttaaatcca
     8101 atcatatata cattaagaca aagcgcaaaa attgaaatta ttaatggaat catttctctt
     8161 cacttacctt tagttatcgt gtgaataatt aaaaaaacaa tagacagcaa cggatgatag
     8221 ataataagaa caatttagaa ggagttgaaa ttcacatcct tattcttaat atttccgttg
     8281 ctgcctatcg aagcttaatt aaacgatgag ggagatttcc tcccttacat tttattttgt
     8341 cgatcctgtt tcctaatttt tcgacactat cataatacaa cgttgaatag gtaagtgatt
     8401 ggtataaaaa aggtataaaa tggaaaccaa attggtaata aaagggtata aaaagtgtaa
     8461 aaactggcta cttgaaagca accagttcta acgatgaagc aaattggatg ataattctgt
     8521 ttgattctac tttaaccgat tcttcgctag tattattcct ttgagcagtt acataaatgg
     8581 gcagaccatt gatataacga tcatagaata tcttcttgcg cctttcagtc acatcaggct
     8641 tatgcggatg ctgtatcgct gaatagcctc gaacaaacaa tttatgcaga tactcaaatt
     8701 cttcctgtgc ttcttcttta tcgatcagca ttctttctgc ttcaaatata tgatcagctg
     8761 tagaaggtgg aaccaaggaa taagatgctg tcacttttgg ttctcgatgt tgccctactc
     8821 tacatctagc tgatagatat gctgaaagaa acacagcgac attatgtttt gtgcgttcca
     8881 tatcaacatc tttcgcgctt ggtgtctcat atttctttac atcgaaaagt accatcctct
     8941 gattccccca attatgatat aatacttatg tcggaaatat tattcatagt cggaggaatc
     9001 cgactttttt tattttgtac gtgaaatgag ttctcctgat ttatacgcct cagcaaattc
     9061 aaccaaagct actgccttca ttctctcgat agttctatcg gaatatccta atttttctcc
     9121 tatttgatac attgacatct tattaggaaa acaataacta taataaagta cctggaaatg
     9181 gattctactt agccgtgtta atgcttcgat aaccgaatct cgagtactta tcgcttcaat
     9241 gatttcttcg ttttgactgt caattgtgaa tttcaaattt ttggtaacag tcatcacgga
     9301 aaagtctatt ttcactggtc cgaccaaacg ttctaaattt ctgtatcttt ttagtaatcg
     9361 tctagcattt ttccttgttt gcttaatatc tatattctct aaaatgttca caattgcctc
     9421 ttctccttcc agttgtggaa taacgctcat tgttgtagga tagttgtaaa aaagcaaaat
     9481 taagatttta taacttccat gtccaccaat ctcaccactg ctaaattctc tttgcttttc
     9541 gctaaccact tatcacattc catcgtgttt tcaatgcgaa tgattgctga gtgattatag
     9601 acgtgttcta catatccacg aaatggatag atgaatcctt ctgcttcgca gcgaaccatg
     9661 tcaccgactt tgaattttgg tttcttacgt gttttaggat tctttgtcgg catatctagc
     9721 attaaaccgc cgatgccgtg gccgctggag taaaatccgt cttttagttt cattccgctt
     9781 cctcctcttt agctgtcaat tacttactcg caagtcacta aaatggctac ttcatcgata
     9841 aagtttccgt cttcgtcttc cattccttgc tgaatgctaa tgtcggtaat ctgcttaaaa
     9901 aattcgttgt catagccttg tgcgtttatc actacttcga cgtctccgaa aacctctttg
     9961 agactattaa ttttatttac tacttccgat gcgtccatga ctacacccct ttccagttaa
    10021 aaatctctgt cgttaataga ctactgttca aagtattcat cttcactcat ccttctgcct
    10081 cctctttagt tgtcattagt ggactaaata ctacctgttt gaaaaccata atgtgcagtt
    10141 tcaacgccgt cataatcact tatttcccac tcagccccat cgggtatatc aacgacttta
    10201 agacttgcat atggtccact ggcctcactt cctagttttt cgacaacagc aacgaggtcc
    10261 ttatcacctc tgtttgaatc acttgaaaaa ctggaatcat cagcaattcc gtacagtcgg
    10321 tcaatttcgc tataaattcc tgtaactttg tcgattttcg ggtctttttt gaaatagtag
    10381 ataaaatcca tcgaacctag cttttggcct gtgtatctcg tgtattcatc atctgagtca
    10441 tagctaaagt tattgatgta tgggaatact tcaatatttt ttcttttaaa aatttccatt
    10501 tttgcttcat ggctcaaccc aaagccacca aaacatttat ttaatacaat cttcattact
    10561 tttcctcctt tgtttcctcc gttagctgaa ttttttcata aacacaagcc aatgtgtttt
    10621 gcttcgttta ttaccaaaca atggctgttc tccaatagcc tctaacacat cagaaagtgg
    10681 tatctgttcc tcgttccact taaaaattaa tgttcctgaa ggtcgtaaaa ctcgcataca
    10741 ctctgcaaaa ccttgcctga tgtcttctgg ccaaagttcg tctaattttc catatttttt
    10801 ggcaagccat gagttttcgc cgacatgtct taagtgtggc gggtcaaata caaccatatc
    10861 gaacacatca tcttcaaaag gcatatcgcg aaagtctgcg accacatctg gatcaacatt
    10921 gatcacatga cctgttgata attcttcata ctgctgacga caatccataa atgttacgtc
    10981 agaattgttc ttatcaaacc agaacaatct actaccgcaa catgcatcta atatttttgg
    11041 caattactct acctccaata actctggatt ctcgtagacg ttacctaaaa cagtgagaaa
    11101 tctttctgta tcccgataaa taattagctt acttatcttc cgaaccattc gactctcttg
    11161 ttgagataat tcgtccatgt cacgatcacc tataatttgc aacattatct gacgacagat
    11221 tcttttttct tgtttagtca aattcatgcg atcgctcctt tgactggttt tatgcgcttg
    11281 tctgctgttt gagaaaatac catcgtaaat ccatcagagt tgacaaacat tcttgatatt
    11341 gttctcgtac cgtaagcttt tttcagttct tcaccaagca agttcgttgt gataatcgtt
    11401 gctaggttct gtcgtgcatc caagaacgaa tttagcgtgt ttatgccaaa cgctcgacta
    11461 tctgatgcat cttttcctag ttctgaaccg atatcatcaa tgatgactaa atccgctgtt
    11521 ttgatatctg caatcaacga tccctcaatc gtttttctga gttcaggatt gttgtacgaa
    11581 aatttgattt gatccagcat ttcctgtaac ccgataaata agattttttt atcgtagttt
    11641 gatcgcttca agacttccca agccgctgcc atcgctaggt gacttttacc tgttccttgt
    11701 tttccagtta aaacaaggtg acttggattt cctaacaaga ctgaattaac aaagcgtttg
    11761 gttacttcaa ccgcttgtct tgtttcttga tcgacaattt gataattctg caatgtgcaa
    11821 tcaaacaacg cttgattagg cacaacagaa ccacctttga aaaaattgat tgctcttgct
    11881 ttcaggctct cgttgtaaat tcgttctgtt tgtaagtctt cctgaactcg caatgcttta
    11941 tacccacatt gcatacaggt tggcttacaa cgttctgagc catcagggtt cttcgtacgc
    12001 catccataca aaggctgtcc gcattcagga cattcgccac gttggacaag cactttctgt
    12061 atcagctttt ccatgatttc cccaacagtt tccatgccta cgcctcctct caaataggca
    12121 actcatctgt gctaaatttc tcgtattcaa gcgttttagt ttgttttgtt ttataatcac
    12181 gatttgcttg cgctgccatt gtgtcgtatt tttctcgcaa ttttttagca gataaaatat
    12241 tcgatgccca aaacacattg tgctgactcc attcaatcat tccccgtacc tggttttcgg
    12301 tccgcttgtc gatctcgatc attttccgaa tgtcatctgc ccaactttgc agattcggct
    12361 ttttgatttc ctgattctga caaatctgtt tgaataactc ctccgcgaga atgtagtaaa
    12421 ctgagtcggt gtcgtaaaca cgctttttgc gtggttgcga cgatgatgtt ttattattct
    12481 tttcattctt ataattcttt tcattcttgt ttgtgtgcac ttgttgttca cttgttgtgc
    12541 gtttgatgtt catttgttgt tcactgcttt ggtacaaaga ccagtttttt attgatataa
    12601 cgctgtattt cgtagttgat ttgatgttca acattccgtt tttttcaaat tgttttagcc
    12661 atctccatac agaaccgctg ttcacttgat gttcacgttt cacgccttcg ttcatctcag
    12721 atgtgatagc atcgcgccct gtgacgaatt ctccgctgtt cacttgtatc tcttttccat
    12781 taaaaagtat cttgcgattc tcatgactcg ctttcatcaa acacaagttc cataatttat
    12841 acatataagg attggtccaa acgaatgaat tcatcacttt tcgatgtaat ttgacatatc
    12901 ctgtgttcat tcgtatttct cctctagaaa agcaagggag aaagctccct cattatttgt
    12961 ttaacggcgg attagatgca tcaaataatc cagtttgtac atcttcattt tcttcagata
    13021 taacctctgc ttcttttctt tcaggaatat cttcttcaac ttctgtttca gcaataatgc
    13081 tgccgtcttc ttgaaccctt tggactctct catccgatgt ggtggcttct tgcatttcga
    13141 tggacaagat tccccattta gaaagaagat ttctcaaaac agtttttcgt gccattgcat
    13201 tgtaatcaga tgcccacaca ccacttaact ttgtcttatc gcgatcttta ttgttagcaa
    13261 tccgatgagc ttcgatttct tgtttggtcc aatagacagt ttttttgaat ccattcagta
    13321 actcgaaata gccaacatat ccaatgactt catcagacgt tctaccattt ggatcaaact
    13381 caaactcttc tgtcagtcgg ttccagcttt ttagttctcc ttcgtaaact tcgatcacat
    13441 ttaatgcttt gtatttacct gatcgttggg ctaattggat atatccttta tagccaagca
    13501 tgaattgagc tttcttttcc cattttcctg tttgcttgtt tttactattg aatggaacta
    13561 aatatgcata acctaaattc ttatctagcc caagatttaa tgttgcagca gttaacgcac
    13621 cactcatgat agacatcggt tcactatctg caagataact gtcattagat acaagagtca
    13681 taacattcga cataaaagca ttagcattgt catgaagcac ctcttcgaac ttctttctca
    13741 ttgttggtgt attcatcaaa gctttaagtc ctaattgacc tggtgcaact tgtttctgtg
    13801 gctttgctgc caattgattt tttaacgatt catttgttgc catattattt gatctccctt
    13861 tcggttagcc ttcttgattc agtaacgtta taaatctctt catcatttgc gacatctgga
    13921 tatttctctg ctagtttctt cgagttcata cgtctcgtac ggacaaattt ccaactgatg
    13981 atgttttttt gagtgatacc gatactggct tcacgtttac ctagctcgct gataatctcg
    14041 ttgtctactt gacggatagc tgattcaatt tctttcttag tccgtttgag ttcgtttttc
    14101 tgttcaacta gttcatcgaa atgaattggt aacgtcgttt gaacgtcttc aacatctgca
    14161 tacttctctt tcaagaaatc agctgtcgct tgactaccat caataattgg ctcgatacct
    14221 ccaagaacgt ttgtttccca aaactctact aattgttcag tgattgtatc gatcagctct
    14281 tgatctcgtt caatccgctt ccagataaat ttttgaccac cgattaagac agcgatgtaa
    14341 caatagtctt tgtttagaac gttcatgtaa tgttgaactt gacagagata gctaagcggt
    14401 acctcttcgc cttcccattc tttaccgaga aattgattgg ctgttttgca ttccagaatg
    14461 gcattttctc ccactacttc ccgatcaata tttgctctta aaaatggatg taatggatgt
    14521 tcaaatactt ggtttcttct gcgtactttt ttgcctgtac gttcctgaaa ctctttagca
    14581 accacttctt ctaaaacatt accccaataa gcgggctcac ttcctgattc ttcaagtact
    14641 acttgtcctg ttttttctag ccatagttga taaggagatt tccatttatt caatcctaaa
    14701 attgttccaa catccgaacc tccgatgcct ttcttacggt cttcaagcca ttcttgatgg
    14761 ctcatttcta aggtagattt actcatcttc atcctcctca tcaattggtg cttcatatgg
    14821 tggtttagca taatcaggat cagttaaata gttgtcaaga tttgctaact cgttcatgtt
    14881 taacctcttt tctgcttgct aacacgcaaa gcacaagcat ggtaattgcg aataacaagc
    14941 caaagattac ataacttttc gctacaatca ctaacgtgaa aaataaaata atgagtagat
    15001 caattgtttt ttcattcatc attgaaattc ccccttgaga atacgagtca tcacatcagc
    15061 taaatcttcc ttgttattaa taacaaaagt atgatttgaa ggttgatttt cttcttcatt
    15121 tttcgcttgt tcgtaagcat ccacagcaat tctacgcata atatgtgctg acaatgggag
    15181 attttcatct aaatgttctt cttgcatagc aagacaaagt aaaatttctg ttgtctcacc
    15241 ataaacgaga cttttaagtt ccccttcttt ttgggctgta caaataatag aaacaccttg
    15301 cttttggcat tcacgtttca attcttcgat aagattttca attttcttat tcatgtgata
    15361 taattctcct agttttataa atttgtatgt gactctttgc tggctggcag agtcactttt
    15421 ttatttgttg ccatgctttt tgcttttcga tatgttgctt gcttaaaata atagggcggc
    15481 tatttcccca ccgattatca gcaatcactt taccgatttt tagcgcttct tctcgtgcca
    15541 tagttgctcc tttcttttga atcaagcaga ttgattaaaa ccatcaatgc tgcgaacaag
    15601 cttcccccga taatactttg gtgcgctaca atcactaata accccaaaat gaatcctata
    15661 aaaagtgtgt ctgtcttctt cataatctaa tctccctatt ttttatttct agcattctca
    15721 aatcctcaag ttcagaagca attagttcag cttgcctatc tgatagctca tcggcttttc
    15781 taagcgctgc acggtcatct tgtaattgtt tcctgcgttg tttaatcaaa ccgagaattt
    15841 gatgttcttg ttgcaaggta tatgccatat cacttctcct ttcgtttatt gaatttctcc
    15901 atttctattt gataaaatat ttctatagaa atggaggtga aaactatggg aaaaaatcaa
    15961 catgttgtac ctaattccaa tggtggttgg aatgtcaaag gtgctggaaa ttctaaagcg
    16021 actgcacata ctaatacaaa atcagaagca gtaaaaatcg ctagagagat ttctaaaaat
    16081 caaggatctg agctatttat tcatggcaga gatggaaaaa ttcaaagccg tgatagtcac
    16141 ggaaacgatc cacacccacc aaaaggttaa tcatatttag gagttaatcg aatacgataa
    16201 ccgtcagcag gagttgcatc ggtcaatgta acttctgcta cttttttccc ttcttctgtt
    16261 tcaataatta atcttgtgta attgttctca ttcaaaatcc ctatttttga ttcttccttc
    16321 atgtcatccc tccttcgtct ctcgccgtca ccacctccaa gaagaggagt tgccaaaatg
    16381 aaaaaacgtg gactttggca aacttgaaag gagagcagca actcctcttt tcagaagtgg
    16441 tgaatttaga ttttttagct attaacaaac agctctaact ctaaatcgat ttttagaacg
    16501 ccatgttcta aactgttcat ataaatcgac atcaattgtt atttctttgg aagtaactgc
    16561 gttatacccc tcactaaaac gtgatttttt aaattccttc attcttcttt gataagtact
    16621 ttcagaatac ttatatttct ctttaaactc cgctttgctc aaacgttgta ccatttttac
    16681 gcctcctgtt ctgatggctg atcattagaa tctacttcta acttgatatc caaaagttct
    16741 tcgatttgtt ttaaacgttc cttaggtttt cttttttcga tgaagatatc tcgtaaataa
    16801 gtaattgata tacccattcg tcgagctaac tctctttgac tccaaccttt ctttactaaa
    16861 gcaattaata cttgctgttt taaagtcatg cttcataccc ccttatcgaa tattttctaa
    16921 tatttttgtc taataagttt gacaaaaact gcaaatatgc tattatgtaa gcatacaaaa
    16981 tacagcgcac aaaaagcgag cttactgatg tctggggaga ctattagttt attgctttta
    17041 gtctttgtat gccgtaaata tcttgcttac aaaactagta taactgcaaa ttctctattt
    17101 gtaaatagat aatttgcaga aaaatacttt ttttgtcggt attttgaaag gaatgcatat
    17161 cctatgctat tagaccgtat aaaaatctta ttaaaagaac gttcaatgac gttggcagaa
    17221 cttgaaagaa agttaaattt cggaaatgga agcatgtcta gatggaataa aagtactcca
    17281 tctggagata aaatacaaaa agttgcagat tactttaatg tatcaactga ttatctttta
    17341 ggacgtactg ataaacgcca ttattatgat ttaacagaaa aagataaaca agatattgct
    17401 ttacaagctg aaaaattact tgaaggcttg aattcagatg ctgaaacaaa ttattatggt
    17461 gaacctttag acgatgaaga taaggaaaaa ttatacgatg ccattcaact tgcacttgcc
    17521 ttaaccaaag ttaaagcaaa gaaaaaattc actcctaaaa aatatcgtca ataggtgggc
    17581 tttggtatgc atttaaacaa atactggata ttagaagaaa tagaaaaatt atttgtagag
    17641 tttggatctg atgcgattaa tccatataaa ataatcaaat attctgatat agatttaatt
    17701 gaaactgatt tagggaataa aacattagga caaacgatac acaataaaag atgttatgtg
    17761 atattaatta atcaaaacct atcaccttct atgaaaagat ttgtattatg gcatgaaata
    17821 gcccacgtta gactccataa aggtatttgc acagcagctt ttagagcaaa caaccttagt
    17881 tgtatgattt atggtataga agctgaagct aatatgtttg caattgaaat gttaactaga
    17941 acaatagatg aacaagattt atctgatatg agtcagttcc aaattattga atatttaggg
    18001 cttcctcata atcttgaaca tttagtatta caacattaaa aataaaaaag cccatgcttt
    18061 cacatggact ctttcctcat ttctgagtaa actctacaat aattatatca aagaaatgag
    18121 gagttttgaa tgttattatt gattggactt attttattaa ttataggaat cactaaactg
    18181 tcgaaagcta acacaataaa aaaagaagct tatgaattag aacaaaaaaa tgatgaaaaa
    18241 ctaaataaat tacatgatag agaaagattt atattaagta aggaagaaac actatcagat
    18301 ttaaataaac aaattactat gttaaaaaat gatattattt tattaaaaaa agagaaagaa
    18361 gatttaaatg atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca
    18421 cttatcgttt caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg
    18481 ttaaaattaa aagaaaagga atatattatt gacgatg
//
LOCUS       DASNNU010000053        18504 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.053, whole genome shotgun sequence.
ACCESSION   DASNNU010000053 DASNNU010000000
VERSION     DASNNU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.053"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..746
                     /locus_tag="U8116_001738"
     CDS             99..746
                     /locus_tag="U8116_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN8434733.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="U8116_001739"
     CDS             1139..3784
                     /locus_tag="U8116_001739"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEN8434734.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="U8116_001740"
     CDS             4094..5407
                     /locus_tag="U8116_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEN8434735.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="U8116_001741"
     CDS             5397..6050
                     /locus_tag="U8116_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN8434736.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="U8116_001742"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="U8116_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEN8434737.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="U8116_001743"
     CDS             6947..7147
                     /locus_tag="U8116_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN8434738.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="U8116_001744"
     CDS             complement(7401..9080)
                     /locus_tag="U8116_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN8434739.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="U8116_001745"
     CDS             complement(9082..9294)
                     /locus_tag="U8116_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEN8434740.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="U8116_001746"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="U8116_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEN8434741.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="U8116_001747"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="U8116_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEN8434742.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="U8116_001748"
     CDS             13269..13466
                     /locus_tag="U8116_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434743.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="U8116_001749"
     CDS             13619..14104
                     /locus_tag="U8116_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEN8434744.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="U8116_001750"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="U8116_001750"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEN8434745.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="U8116_001751"
     CDS             14981..15508
                     /locus_tag="U8116_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434746.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="U8116_001752"
     CDS             15599..16336
                     /locus_tag="U8116_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN8434747.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="U8116_001753"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="U8116_001753"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEN8434748.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="U8116_001754"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="U8116_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEN8434749.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3199 c         4022 g         5299 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgt
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DASNNU010000054        17583 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.054, whole genome shotgun sequence.
ACCESSION   DASNNU010000054 DASNNU010000000
VERSION     DASNNU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.054"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            68..808
                     /locus_tag="U8116_001755"
     CDS             68..808
                     /locus_tag="U8116_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN8434750.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="U8116_001756"
     CDS             complement(1232..1402)
                     /locus_tag="U8116_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN8434751.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="U8116_001757"
     CDS             complement(1596..1775)
                     /locus_tag="U8116_001757"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434752.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2162..3274
                     /locus_tag="U8116_001758"
     CDS             2162..3274
                     /locus_tag="U8116_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN8434753.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="U8116_001759"
     CDS             complement(3352..3645)
                     /locus_tag="U8116_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEN8434754.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="U8116_001760"
     CDS             3813..4127
                     /locus_tag="U8116_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEN8434755.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="U8116_001761"
     CDS             4148..6127
                     /locus_tag="U8116_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN8434756.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="U8116_001762"
     CDS             6140..6466
                     /locus_tag="U8116_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEN8434757.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="U8116_001763"
     CDS             6482..7000
                     /locus_tag="U8116_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434758.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="U8116_001764"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="U8116_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN8434759.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="U8116_001765"
     CDS             complement(8436..9941)
                     /locus_tag="U8116_001765"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEN8434760.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="U8116_001766"
     CDS             complement(10033..10623)
                     /locus_tag="U8116_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN8434761.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="U8116_001767"
     CDS             10802..12448
                     /locus_tag="U8116_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN8434762.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="U8116_001768"
     CDS             12497..13216
                     /locus_tag="U8116_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN8434763.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="U8116_001769"
     CDS             13695..14642
                     /locus_tag="U8116_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN8434764.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="U8116_001770"
     CDS             14654..15574
                     /locus_tag="U8116_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN8434765.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="U8116_001771"
     CDS             15615..17072
                     /locus_tag="U8116_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN8434766.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..>17583
                     /locus_tag="U8116_001772"
     CDS             17417..>17583
                     /locus_tag="U8116_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN8434767.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYK"
BASE COUNT         5518 a         3013 c         3588 g         5464 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 aga
//
LOCUS       DASNNU010000055        16612 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.055, whole genome shotgun sequence.
ACCESSION   DASNNU010000055 DASNNU010000000
VERSION     DASNNU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.055"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..815
                     /locus_tag="U8116_001773"
     CDS             <1..815
                     /locus_tag="U8116_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN8434768.1"
                     /translation="GDTAGKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFV
                     IDFIVNNGQVSDTVANLHGQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEH
                     HEKTKADVVTYGAKFLKVDSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGV
                     KEAVWVDDVANAAILKSDKEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKD
                     SYKEENRITIKNNQKASVPMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            828..1583
                     /locus_tag="U8116_001774"
     CDS             828..1583
                     /locus_tag="U8116_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN8434769.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            1580..2404
                     /locus_tag="U8116_001775"
     CDS             1580..2404
                     /locus_tag="U8116_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434770.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            2705..4804
                     /locus_tag="U8116_001776"
     CDS             2705..4804
                     /locus_tag="U8116_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN8434771.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(4842..6899)
                     /locus_tag="U8116_001777"
     CDS             complement(4842..6899)
                     /locus_tag="U8116_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEN8434772.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(7013..7225)
                     /locus_tag="U8116_001778"
     CDS             complement(7013..7225)
                     /locus_tag="U8116_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434773.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            7412..7879
                     /locus_tag="U8116_001779"
     CDS             7412..7879
                     /locus_tag="U8116_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434774.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            7876..8118
                     /locus_tag="U8116_001780"
     CDS             7876..8118
                     /locus_tag="U8116_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HEN8434775.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            8136..8438
                     /locus_tag="U8116_001781"
     CDS             8136..8438
                     /locus_tag="U8116_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434776.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            8510..10564
                     /locus_tag="U8116_001782"
     CDS             8510..10564
                     /locus_tag="U8116_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434777.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            10564..13176
                     /locus_tag="U8116_001783"
     CDS             10564..13176
                     /locus_tag="U8116_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEN8434778.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13173..15704
                     /locus_tag="U8116_001784"
     CDS             13173..15704
                     /locus_tag="U8116_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434779.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15756..16088
                     /locus_tag="U8116_001785"
     CDS             15756..16088
                     /locus_tag="U8116_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434780.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16089..>16612
                     /locus_tag="U8116_001786"
     CDS             16089..>16612
                     /locus_tag="U8116_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434781.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILG"
BASE COUNT         5950 a         2603 c         3252 g         4807 t
ORIGIN      
        1 ggggagatac agcaggtaaa ggactttctt atgttgctga aaaagcagat attaaagtaa
       61 caaataatga tggttcagtc gtattaaaag aaaatactga ctataaaatc gaaaattctg
      121 atagtggttt tgtgatcgat tttatcgtaa ataatggaca agtaagtgat acagttgcga
      181 atttacatgg tcaagatcta aaaatgactt ataacatgtt tgtgaatgat agtgctgaaa
      241 tcgcaaatcc attaacaaat agcgtggatt tcgtttataa caataatcca tttaatcagg
      301 aagaacacca cgaaaaaacg aaagctgacg ttgtgactta tggcgcaaaa ttcttaaaag
      361 tcgatagcgg cctatttgga acaggtatca aagcaacacc gcttgaaggc gcagaatttg
      421 cagctaagaa tgcagaaggt aagtactacg gtggacttac tgatacagat aaagatggtg
      481 tcaaagaagc agtttgggta gacgatgtag caaatgcagc aatcctaaaa tcagataaag
      541 agggtcattt tgaaatcact ggtttaactg aaggagatta ctcactggaa gaaacaaaag
      601 ctcctgaaaa ctatcaaaaa ttaacaaaag agatctcttt caaagtagac aaagattcat
      661 acaaagaaga aaaccgaatc acgatcaaaa ataatcaaaa agcttccgta ccaatgactg
      721 gtagcaacgg tttccaatct tacgtattga tctcttgctt gttgctagga gcagggggac
      781 tttctgcagt tgtttacttt aagaagaaag cctagaagga tcagctaatg attattcgta
      841 agcccccaaa aagaagagtc atgcacaaac tgatgatatt tttttggttg ctgtcggcag
      901 tagcagtcta tttaattcca gttgtggaaa caaatgtagc gaaatttcaa caacagcaaa
      961 gaattttagc cattgaaaat cttgaaaaaa cagattcttc tttggatgtt gcgaaaatag
     1021 agttaggcga tcctgttggt gttcttacaa ttccaagtat ttctttaaaa ctaccaattt
     1081 atgacggaac ttccgacaaa attttagaga atggtgtagg gattacggaa ggaactggcg
     1141 atattacagg aggaaacggt aaaaatcctt taatcgcggg gcacagtgga ctttataaag
     1201 ataatctttt cgatgatttg ccttcagtaa aaaaagggca aaaattcttt attaaggtaa
     1261 atgaagaaca acatgcctat caaatttatc gaattgaaga agttaaagca gaagaattac
     1321 aaaaaaatta tttagattat cttatgcctg aaaaagacca agataggatc acgttaatga
     1381 catgcacacc aaaaggtgtc aactcacacc gctttttagt gtatggtgaa cgagtttcct
     1441 tcagtaaaga agatttaaaa ctacagaaaa aagcaaaaaa taggaaatca atttcattgg
     1501 attggcgatt agcactgcta ttcgcttttt tttgcgttcc tattattttt atttataaaa
     1561 aaataaggag gagatcggaa tgaataagag aaaatggctg tttgtttgtg tagcagctct
     1621 cttttctact gtagcttatt cgcaactgtc tttcgctgac agcagtgata gtagtaccac
     1681 tcaaacaata gataattcaa ttactgcacc aagcgaaacg gacagttcgg agaatactac
     1741 agaagaaacg gcagattcaa atataagctt tactgaatca acgaatccaa cgtcagaaag
     1801 ttcttcagag acttcagaaa agccgcaaga cgtagaagat acgcaagtga tcaagcaagc
     1861 cattcatatt caaaaaatta tttcttcgaa tcaaatcaac aatcaaggca gtcaagtagc
     1921 ccagcaagaa ggtatcaatg gcgcgaagtt tattgtctac gatatcacga atatcttaga
     1981 agaaatgtca caagatgata atgagaaaaa tcaatctgta gaaataattg agtcaaaatt
     2041 gaaagatcga gcaaaaaaac tttcatacga ccagttgaaa aaagtggctg aaggagaaac
     2101 caaaacgatt gatgatcagg aaggcgtgat tgaattttcc attgaagtac ctgcaaacaa
     2161 aaaacaagca tactatatcg ttaatgaatc aagccctgaa aacatttcaa acagtgaaga
     2221 tattgtttta ttgacacctg tttcaaatga aaaaggtgaa tttttgacgg atgtttggtt
     2281 ataccctaaa tcagagaaat cagagccgaa agaagaaatt aaaaaagttg tgtcaactgg
     2341 tgtaaagaag aacttttttg aaaattgttg ggaatttttt gctcaattat tttttgataa
     2401 ttaggatgaa atgatgaaaa taataagaat attagttttt aatctatcct ttttagccgc
     2461 aacaatattg tgttgcggtt taattttttc tcaattatca atttatccaa gatatcaacg
     2521 attgttcatt cattttctaa acattcaatg tgcttccatg ccattagagg tgattaaaaa
     2581 gctcttgatc aatccaattt ttatggttag tttggttctt cttacaggat tggtaacgtg
     2641 ggtattagga aaaaaagaat gaaaagctta cgatcgttta aaaaaatatt agatggaggg
     2701 cattatggaa aataaaattc attgcactac gggaagagta attactttag tgttgttttt
     2761 gttccttctt ctttttgcga ggggaactgc agtccaggcc ttagaaaaag ttgatccgag
     2821 tcaaatcaaa gaaaataata atctggtgct ttatcatgat agtgattcat tgagaaatat
     2881 cgaagtaact gggatcaatg gatttaagat actagatgag tattcaacta aaaaacaaaa
     2941 agtacttgct tggccaaaag tcaaagattt gaaaagtacg tcaaaaatcg ttgcccatta
     3001 tcaaaaagta ggtacttatc taggaaaaga aattgattgt gacgtgactt tttctaattt
     3061 tgtagcgcac gctggggtta accaagacgg attagctaaa acaattaatg gcaaaaagta
     3121 ttataattat ttgctctttg ataaaaatat ttatcgagga tttttcatgc agtcaatcaa
     3181 tgtcatgaat gcaaaactag aatttaagta tgcaggaaca aaaacaaaag tagctttgag
     3241 tgaaccacag ccagaaattc aatcaaaaat gagatcgatg agagcagctg gtattgattt
     3301 aaacgataca tttctatcca tttacagttt aaatggacag tatcagcaac gtgtgtcttg
     3361 gtataatgac aataatgaat ctgtcggtga acgtaatatt gatatgagag aatttgttgg
     3421 gtatgaaaaa atgggtagct taccttattt tgtcacaaca gatacagcat gtgcagaata
     3481 caaagctcct gcgttatcaa caaacaattt aacttcaaaa gtagtgggag gacgtgcaga
     3541 aaaggcttat agctcgaatg atcatttcac cgatgttgta ggagctgata cttatcacag
     3601 aagtggtgta acgtatacgc ttcaaggcgc ttccccaaca ttcatgattg gcgcaaatac
     3661 gaatagtatg atgtttagct ttgatactgc attgctatgg acaccacaac catcgaagcc
     3721 tacaaaagaa gtgtttaaca aagctaatac tgaagaggca gcacacaata ttgacaaaaa
     3781 agtgattcca caaggatcag atgtttacta tcatattcac caaaagtttg atgcattaac
     3841 agtcaacaca atgaacaaat acaaatcatt taaaatcact gatacctttg acagcaaaaa
     3901 ttttgatatg gtatcggatg ggaaaaacta tgatggcgca ttgtggtatg ttgaagcgaa
     3961 tggtacttgg aaaaaagctg atacaggagg aaagttaact gtatcaggta atacagtcac
     4021 ttatactgct tctgacagct ttttagctaa cgctgcaaat tacggtaggg attatatttt
     4081 gcagctccat atgagagcaa aaaatgatgc aaaaggaatt gcaccaaatg tggctactgt
     4141 aacgtttaac aatgatccga acaaaggaac acaaaaaaca aatacagtag aaaactattt
     4201 ctctcttaaa ccaaataaaa aagtggaaca agatgggaaa gatgttgatg gtcgaaatga
     4261 tggtaaaaaa ggaacgccta cagctccttt aaatgcagga tctgaagtac aatatctagt
     4321 cacacaaaaa tggcatacaa aaggtgtaga cattgcctca gatcattaca agcaatttag
     4381 tattcaagat ccagtggaag atcgtttgac gtataaagaa ggttctgcgc gagtgataga
     4441 tatgagtaca ggaaaagata ttacttcaga aggtacacta acctatgata gcaattcaag
     4501 aacgctgaag tgggaagctt ccgctgattt cttaagtaaa aatcttttag atggacgaga
     4561 aattcagctg atatttacag ctaaaactcc tttgaatgaa gaattggata tcgacaacca
     4621 agcaaaagtt tcagtagaaa gtattgagaa agcaactaat gtagtaacga ttggggtaaa
     4681 acctaactta cctcagatca ttgtgccaaa aactggatcg aatcatttag tgctaactgt
     4741 tattggaagt atattatttc ttactttagc atcattgggc tatgtagttt taaaactcaa
     4801 ataaatataa aaacaaggct gagaatcagc cttgttttct tttatttgaa gattggattc
     4861 gcactaataa atacattatc tacgccaatt ctaccagcag agtcaactac aaaattatga
     4921 cctaatccga gaatactttg gttattataa agtaaatttg cattatgggc agggctattt
     4981 ttccatccta ccatggcatc gatagcacca tcgtctgata aaaggtcatc aatatacagc
     5041 ccagtttgac ttatgttttc tcccttagca ccgtagcagc ctaagctttc caataaatct
     5101 tggaaagtac caccaccagg gcgatagtga ctgaagctga cttgtaattc tttagctcga
     5161 atatctgcag cctgttgaac aatatcattg aattgcattg gggcaacacc attttgttgg
     5221 cgataatcat ttaaccaagc aagttctttt tgacgcataa agttacgtaa ttcgtctaat
     5281 gaaatctttt tatatttgaa tgtgattgtt ttattttgat cgacgttatt aatggatcgt
     5341 tggccgtttt tatccacggt aacggtagga tcgtcatttc tgcacaagaa ataattttga
     5401 tcagtcatgt ttttccaagg agtagcaaag ctatcccctt cgtatactgt ttttgattca
     5461 ctagagagaa cagcgttggt atcagcattt acatgattaa cagtcacatt aaatttacga
     5521 gtgtagttga aaatataatt ttgactttca gtcacattgt ttaaggttac tgaatttgtt
     5581 ggttctaccc gatcgtctaa aacatatcct tgaatagaag gagcagttaa agtgacactg
     5641 tcaccgtgag tgacattgac tatctgttct ttcaaaatat taactcgtcc ttcgtttttg
     5701 tagttgttat tgcacaacaa attgatttga tgcacttgat tataattgaa tgtgattgtt
     5761 gtattacctg tgattgtttt gctttgagta tcattaccaa tcactttgta gctaaaatta
     5821 tctttagcgc cttcgctata tttaaatgct tttgccttag cagtgtaagt agaaccagaa
     5881 gttacttttg cttgatctgt agcaatcact tgacctgttt gattgtcaat atggttaata
     5941 gtaaccgtat attcttttgg tgtttcaggt ttgtgttgca accctttaaa tgcagcatca
     6001 agactatttt tagcattgtt tacttgtgtt tgagaaacct tagaatcgtc tagtactttt
     6061 ttagcattgg ataaagcagt ctgaaaatta ttccaagatt cttctgtaaa atctccttta
     6121 gcagttgatt ttactcggtt gtataaagct tgtaattcag ctttattcac agtaggaact
     6181 ggttttttct ttaatccttt gtaagcagaa tctaatttat ttttggcact atccacttgc
     6241 ttttgagtcg cttttgagtc atttaacact ttcttagcat tgtttaaagc ggtttggaat
     6301 gcgttccagg tattgtcggt atagtcacct tttttcgttc ccttaacgct cgtataaaga
     6361 gcttctagag ccgatttatt cactttgggt ttagaggtgt cagcgccata ccaaccgata
     6421 ccttcgtatt tccagccata gtctttggtt aaccattgta cttctttcca acttgcagtg
     6481 tagtgatgtg agccagattt tacaccagga aggaaaaggc ggtagatcgg tttattattt
     6541 ttttcggcag aacgccaggc aggcccttca taagtccagc cgtaattttt agtcaaccat
     6601 ttcacttcat cccaactagc tgtgtaatgg tgatcacgaa gacctttatt gtacaaacga
     6661 tagacaagat ggccaccgct agtcggtgct tcccaggcgg ggccttcata atttccccat
     6721 tttatcttta ctagatggtc acgttctttt ttgctagagg tgtaaaaatg ttcattatta
     6781 tttggattgt aaaggcgata catgacgtta gttgaagcag cgtcaacaga tccagcaaga
     6841 ctaagtgagg cagcaaatgt tccaatagct aaaacagttt ttggattaaa taatttcatt
     6901 ttttcaactc ctaaatttaa ttaaaaatgt ttacatgatt attatatatc ggctaaccga
     6961 tatagtcaat aaaaaataag aaaagcctta tataattttg gcttttctta ttttatttgt
     7021 tagttgaagt attaatatca acgatttctt cagccaattt atttaatttc atagcattta
     7081 tgaatctcat gttgtctata ctagagcgtt tgttggcaag gtcacttact gtagttaagg
     7141 gtacgccaga ttctatggat atagaacgaa ctgatttacc actatcaaac agcaaccatt
     7201 cgattttctt tttatcagcg tacatttcaa tgctccttct tgttttttct ttattatagc
     7261 tcttttttgt aatgaatcac aatatcaaga gaaaaatttt ctatagaaag gtggattttt
     7321 atgagtattc gttctccgtg tatccaaatg aggtttgttg ctactttcgt tatttgatat
     7381 actaaaaata aacaacgaaa gaaggaataa aatgaagaaa aagcaactat ggtttgtctc
     7441 tatgtttgca atatttttta ttattttaag tggatgttca agtaataaga aatcggaagc
     7501 taaagaagat aatagtttat tgcaaccagg aactgtttgg aaagaagaag taggtggtct
     7561 tattcataat ttaaaaatta tagatgatac tacctgggaa tattctgaaa gtgtatggca
     7621 tccagatcct gtacaaataa cagttaagag gcaaaaagac tatgaaggtt tagaaagata
     7681 caaaatagta gattctgcag gcattaggga atttataaac aaacctgatt cattatttat
     7741 tgttgttccc tatgagaagg atggcgtaaa aaaaataatt tttcttggat ctagcaagga
     7801 tgagaagcga acgaaagaga aattaattca tgatggatca gagttaaata aatacaaatt
     7861 acaaaagact agtgagtgat tgctagtctt tttatttatc ctgaaggagg aatcacagtg
     7921 gcaaaagtta aaacaccatt cgaaaaagcc gcgtctaaaa tcttagaacg ccgtggtgtt
     7981 tctgaaatac agtggaaaaa agaagttctt gaaaaagcac aatttgacct attacgagga
     8041 aaagataagg acttagaaga atatatctat caacgtgaac gtgaaaaact tgtattcgat
     8101 gaaattatta aacattaaaa ggagcgaata atcttatgtt tgaatcaatt acaatgaatt
     8161 ttttaactca accgaaagta gtagctgcag ttgatatttt tagagcatta caaactgccg
     8221 caactagttt gaaaaataat ctgatgattt ttgcggctgt agctgccgtt gcctccattg
     8281 ctcttggagg tattttcttc ttctttgggc gaagaggagc tgaaaacggg aagacacacg
     8341 taggttatgt attgttgggg gtaggagtta ttttagctgc cgcttctatt gtagtttcaa
     8401 tgttctcaat gtttggacaa accccacaag gactataaaa aaaacaagta ggaggagtaa
     8461 aaagtacgtg gctacttttt actccttttt ttatgaaagg agaatattca tgttttttac
     8521 gatttcagga aatgcaaaat tggataaaga gttaataaga aaagcaacga agaaagtggg
     8581 aaagcgtact tcctttttac cgaaaacaat taaatattct gtcattgaaa cggaagaaca
     8641 gctacatgtg ttaggacaag tgttagaaca agagaatcaa tcttctgtta aattgactct
     8701 cacgttacat gataaagaag gaaaaataga aaaggctggc acagaatacg tttttggccc
     8761 actggattat caaatttcag aaggttttta tcctacattg atagatttaa tccgccttga
     8821 tgtgtttaaa gatacggatg aaactatgag tgtggaagag tcttatcgaa acgaaaaact
     8881 gctacaagtt ttagaagaag agttagagag cgaccaaaga aaagtgaatc aagtgagtga
     8941 agtcgaagta aatgaagaag taccatggga acgaccaatc tttccaaaga aggagtcaaa
     9001 agaaaaagag acagtctatg ttgacgaaca agctgatatt ccgactcaga aatcgatatt
     9061 tgaggaagag aacgatgtga tgggtcaatt ggaggtagat ccctttgaag aaacaatgga
     9121 ggatgaagag acggacgaag tagaagttga tccagttgaa gctaatgaag agtcaacatg
     9181 gactgaaggg gattttcagg aatatattga accacttatc agtgaagaag tagaaaaaaa
     9241 cgaaacagag atacaaaagg caactgtatt tgattatatt ttggatcaat atccgccaga
     9301 gcatgaatgg atgaaagaaa aaaacaaatt atttttaaat gagctttatt cgaaatatca
     9361 attaccccaa acctatcaag catttttaga gagtaaagaa gaattaatct cacagggtaa
     9421 aggcacactt ttagaacagt tagagtctgt ccaatcaaca aattggcatg agcttgcaca
     9481 agaagattta ttagccacct ttgaagaacg tcaacaagaa acagatcaag aaattaatga
     9541 gtatgcgacg gaacagtcgt ctaattggga gaaagtaaaa gaggaactaa ataaagaaga
     9601 acagcgagtg attgaagaag aagtacaaaa agtcaaagag cggtacgaaa agaaacgcga
     9661 caatgcttat tccttgtgtg tagaaaagat caacaatttt actaagacga atcaagaagc
     9721 gttatcgaaa gaaaaagaaa acttgttaaa agaacgtatt aaagaacgaa aagaagagtt
     9781 ctaccgcggt ttacgtacag ataagctaaa gattagcaaa gaattaaatg aacaactgaa
     9841 taagctatat caaacaacga ctgaagttct tatggaaaaa cacgaacaga tccagcaaga
     9901 attacaagta caaattccaa aatggaaaaa agagtatgaa attcaacaac aagaattgaa
     9961 agaacaagaa gaaaaacaac ggcaaaaaga aaaagagcgt gaacaattag aaataagaaa
    10021 acgggaacag gatcttgcgg aacaaaaaat ggcgatagaa aaacaacggt tagagcaaga
    10081 aaaggaaaaa gaagcagctg ctcgtaagga acgtcaagaa caaatggata cattgcgtca
    10141 agcacagttg aattatctag cacaacctca accagtagct gctgttcaaa cgccagtgaa
    10201 caaccaagca aatttaactc cttcacccca gcgttccttt aacggatgga tggtgggatg
    10261 tattgcaagt ttaagcttgc ttctaggtgg aggaacagta tttgctttta atcatatcaa
    10321 tcaatcttca caggctgatt ctgtagccac aacggcgtca atcaaggaag aagcccagga
    10381 acaagcaaga aaagagtatg aacaacaatt agaagaatta ttgaatcgta ccaatcagtc
    10441 aacaacagag tcaagcatgg aaaatagcaa agagagtgat cctagtaagg ctaaagaagg
    10501 atcaagcgat tcaactgtac actcaaacac ttccgaaagt tcagaatcaa atgaggtgaa
    10561 ataatgcgta ttagagaaac aacgagacga aaagacaaac gattcaattt agaaaagaat
    10621 aggtatcttt ctcatttcta tcgctatact ccttcaggag aagagctacc agaaaaacat
    10681 gacaaacgta tttctcaaaa gattttttat agtggcttag cttatcttcc tttaaactat
    10741 gttgttacaa gaataccaga agcatttcat cgatttgaga ttattccagg aagtttacaa
    10801 ggagtgaata tttttcagtt acaagaaatg ccgcagttat tccattttga ttcaactggt
    10861 ttttggaatc ctttgacccc aacaacaagt ttaggcacac gtttagttgt ttctacgatt
    10921 tgtgttgttc cagctgtact tatgggattg aaaatggaca tgtggtttcg tccgctagca
    10981 gatggacaaa aaggggataa tcgattaatg accttaaaag aaatccaaca gatttatccg
    11041 caaatacctg aaacaaaaac tacttttcca ggttatggag gtattccaat cacacattac
    11101 aaaaaatatt ggtacattca aacagatact gttaatacgt gtattgttgg tacctctcga
    11161 tcagggaaag gtcagattga agtgttagcc accattgaca atttatctcg tgcagaaaaa
    11221 caaagttcaa tggtcgttaa tgatccaaaa acagaattat atgttggtag taaagatgaa
    11281 ctagaagcta gagggtatga tgtatatgcc tttaatatca ccgatccact gcaatcaatg
    11341 agtgataatc cattggcact catcgtgaaa tactggaaac gaggagacat agatactgca
    11401 acacagttaa ccaatacttt cacaaatacg atttattatg atgccaacgc ttcagataac
    11461 aaatacttta acgaaaacgc gcagaaagct gtcaatgcgc ttatttttgc gttgttagaa
    11521 caagctgatc aaacaggaga ttactccaaa ttgacaccga ataatctcgt tgagcttctt
    11581 tctgagattg gcggttttaa ttattatcca gatccagaca atgaattcaa acagttgaat
    11641 gccttagatg aatttatgaa tcagttgcca cctggaaaca ttgcaaaaaa acaatatggg
    11701 gcaacgaaaa ttggatctga taaagcgaag ggaaatattt tatcaacggc aatcacggga
    11761 ttgaatccat ttactctaac gaaaattgca aagatgactt ctcaatcaac gattgattat
    11821 aaatcggttg ggtttccaaa atacttagat ttgaaattac atgaaagtct tttaaatcaa
    11881 cgagtcgtag tagaatttta tcataaggga aagaaatttc atgaagaacg tgtcaaagta
    11941 ggctataaag gatttagtga aatcaatttt gattgtcatt tagttgcagg tgatcaagtt
    12001 tgtttaaaat acgatcatca aggaaaagaa tatgtggctt ggtatgaatt ttctcaaaga
    12061 gtattgaaag acgaagaggg taatatcgtt tatcggaaaa aacgaggaga aacccatttg
    12121 ccagaattag aaaaagaagc cgttctaaca ttagatgagg aaaaaagtaa cttattagta
    12181 gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgct tacacctgat
    12241 tatgatccat ctaaccatat cattgttagt atttttctta gtcagttata taaagaatta
    12301 tctacgcaat gtgttaagac taaaggagat aaatgtcacc gcaggattca ttttatttta
    12361 gatgaatttg ggaatatgcc accaatggat aatatggaag gaatcatgac ggtcacagct
    12421 ggccgaaaca tgttgtggga tctgtttatt caatcttatc agcaacttta tgcgaagtat
    12481 ggagaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta tattatgtcg
    12541 acagatgata ataccatcga agaattttct aaaaaagtgg ggaataagac ggttgaacaa
    12601 gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa tgttgattca
    12661 gatcgtatct taactcctga aagaatttct acttttttag aaggagaaac gattgtactc
    12721 gctcctttaa aacgacaaga tttgaaaggt cataaaatac ggccgtatcc tatttttaat
    12781 catggggaaa cgaatcttcc ttatgcttat caatatattg aagatttcaa tccatcgtgg
    12841 gatatcaatg atatagacat acattgtaca catgcggact tggatcttaa ggctttacaa
    12901 attgattatg attatttcct accagatgaa tatgctagaa atctctatca tggactgcat
    12961 gatcaattag aagaaaaaaa cgaagaaaat atgttttata aaatcgttca atcttttgag
    13021 aatgagacgg ctaaaaaatt gttaattcaa ttttacgaag aacaagcagc agatcgactt
    13081 caagaatatg ttgaaaagtt gatcaaaaat aaagaaattt ctgaaatgat tgggaaacgg
    13141 ttgttattgg aatctgaaaa ggtggtgaaa gaatgatcat tggaggaatt ttttctggca
    13201 tagccgactg gtggtcaggc ttattcggag aaggggatgc aaaccccaaa accgtaacga
    13261 aagtcttaca agattatggg aattatctgc agtatcaaga tgtatttagt tggatttgga
    13321 acacttttct gtggggattg attaaaggat tatataatct caatacttta ctagaaaaaa
    13381 ccatttatca aagctttgat ttaaaagact tattgaacgc cgctggggta aatgcactat
    13441 ttcaaagttt gattagtaag gtattggctc ttttttgtgt cgtcacatta atttatttag
    13501 gcttgaaatt ttttatgagt aaacacccac caaaagtcaa aaatatcttg gttaatttat
    13561 ttatggccat gatcttgatt tttggtggta gttctttaat cgatgaaggg ttaaatatca
    13621 gtaaaagctt ttacggagac gtcactgcag caaataaagc agataaaacg ggatcacctg
    13681 cttttcaaat tattcaaaat aatgtgatcg atgtcagtac gttactagca actgacccga
    13741 ataaagtaga atccctacca acagataaaa gaaatgcttt aacagcgaaa aattttaaaa
    13801 cagcgaatat caatgggatt attactcctg aacaagctaa aggtatggca ggtaatgata
    13861 aaagtgggtt atcagatata caaaaggatc ggttaaaggc attacaatac cgattggaac
    13921 tagatgataa tgggaaagaa attcctgttc aaattagtga cgaaggctta gcaaaatatg
    13981 tctatacttc agggtatcgt cgatacagta gtaaaccagg tattatttta gctggtgaat
    14041 cttctttggc agtagcctat ttatttatcc tatttacgat cgttacttgc attattgagc
    14101 tggtatttaa aaaattctat ttagtgattg ccgcgtcaac ggactttgag acaggtcaac
    14161 ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg tttacgattt
    14221 tagaattacg aatctatacc ttaatgcttt caggtatcgg tgatttgcat gcggcaggaa
    14281 agatcaatgg attcttgtat gtcgttgctt tgattgtact aaccattgcg ctattcaaag
    14341 gctctcaatc agttacgaaa attttcggcg ttgatacaag cctgaaaaac ggcagtaatt
    14401 ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa
    14461 accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt aaaaatggat
    14521 ttggccatcg aaaagatgtt acccctgata acggggaaga acaatcagac gataaagcta
    14581 caactgtacc taatagtaag aaaacagttt ccgaaatgtt cgggaaagcg aaaaatggtg
    14641 tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagctg
    14701 aagatcttac agaaaaagcc aaagacacct tagaagataa attagacggc acgagagcta
    14761 aagaattggc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag
    14821 cttcagaagt caaagaagga ctaaaagatg cgtatggtga aggacaagtc aatgcagcag
    14881 tgaaagctaa tacaacaacc gaaaaagaaa atcaagaagg tatagtgatt ccttcagtca
    14941 aagatcacca agaagcgttg cactctaatg aaaattcgat tgaagagaaa aattccatag
    15001 aagaaagaaa ttcagaagaa ggaaaaaatg gacagttacc aaaaactgat ccagaacaat
    15061 atcgctataa acctaacagt gaccttgaaa atgagaagcg aatttcagat ttatcaaaag
    15121 taaatcaaga agacaaagca gaaaatgagg gacagttacc aaaaactgat ccagaacaat
    15181 atcggtatag atctgttaac tccgaaaatg aaaaacgcat ttcagattta ccaaaacgaa
    15241 atcaagaagt acaaacggat gaggattcca tcgttctgcc aaatgataat ggaatcgaag
    15301 ttggaactga aaaatcaacg aaaattggtt tacctaaggc acaaaattta ccaatgaaag
    15361 atagtcgttt gaatcaattc aaggataatg atcaaagttt tgttcatcaa tcacctgcag
    15421 gtactgttca aatgaaacca agtcgtttgc ctgaaaatgg aaatctgaca actgtttctt
    15481 acaatttatc tgaaagtggt gagacaggga atgcgtccgt tcaaaatgtt gtgacaagag
    15541 aagctgcacc aaaacctatt caaaacgttc atacaggtta caagccagca aatgtggcat
    15601 ttactgcacc aacaaaaata aatgtgacaa gagaaacaaa aaacaggaac aatgcattga
    15661 aagaacaact attgagggat atcaaacagg atatcaaacc ataaaaggcc tacaagttag
    15721 gccttttttc tatgtatgaa aaggagaaat catttatgga taaatatagt aacatgaaaa
    15781 accctccaag atttagtgga gaatggaagt ttttcgcttt tttcttttta gatctattaa
    15841 cgcttggtgg ctgctttttt tgggcgaaaa tggtcaataa tcttgtccac ttaccactct
    15901 tttttgcact cttgaattat ggcttatgga ttgcgatagc gttagtgatt gtttggcgac
    15961 cctataataa cccaggtcaa cgacaaattt ttgcgattat cgagtctatt ttttttgcgg
    16021 acaagaacca ttatcatgcg ttagatccta accgaaatta tcatggtacg tataaggagg
    16081 atgaataaat gcgagtcaaa ggacaaaatt cagttgaaag gaaatccatt ttgcagtttg
    16141 gaaaaccaga agagcgctct atcttggact tatttccata taaatcctgt acagaagagg
    16201 gagtcttaat tactagagaa gatcattttc aacgttatta tcgtgtagtt tctacagacg
    16261 tggaaggatt aaatgaacaa gagaaactag aacgaatgaa tcagttgacc accgtcatgc
    16321 gtacgtatgt gccaacaatt aagttagtta gtttgaccac agagacggat ctaacggaac
    16381 aaatcgtgca aaagcggcaa ttattagata aaaatcgtat ggaacagtcc ttaggaagaa
    16441 atctacaacg attaaaaaaa tatgaaaaga aattagtcga agaaatccag gaattgaaac
    16501 gagcagaaca agaacgtcct gacttaagct tctttttcgt cattgaagga aagacaatca
    16561 aggatttaca aaccagaaac cgtcagttaa tgcgatctag tggcatttta gg
//
LOCUS       DASNNU010000056        16338 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.056, whole genome shotgun sequence.
ACCESSION   DASNNU010000056 DASNNU010000000
VERSION     DASNNU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.056"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(29..394)
                     /locus_tag="U8116_001787"
     CDS             complement(29..394)
                     /locus_tag="U8116_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434782.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            602..1084
                     /locus_tag="U8116_001788"
     CDS             602..1084
                     /locus_tag="U8116_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434783.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1281..2171
                     /locus_tag="U8116_001789"
     CDS             1281..2171
                     /locus_tag="U8116_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEN8434784.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2496..4967
                     /locus_tag="U8116_001790"
     CDS             2496..4967
                     /locus_tag="U8116_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN8434785.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5070..6806)
                     /locus_tag="U8116_001791"
     CDS             complement(5070..6806)
                     /locus_tag="U8116_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEN8434786.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6803..7507)
                     /locus_tag="U8116_001792"
     CDS             complement(6803..7507)
                     /locus_tag="U8116_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN8434787.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7638..8141
                     /locus_tag="U8116_001793"
     CDS             7638..8141
                     /locus_tag="U8116_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEN8434788.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8101..8439
                     /locus_tag="U8116_001794"
     CDS             8101..8439
                     /locus_tag="U8116_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEN8434789.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8460..9878
                     /gene="ffh"
                     /locus_tag="U8116_001795"
     CDS             8460..9878
                     /gene="ffh"
                     /locus_tag="U8116_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEN8434790.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9990..10568
                     /locus_tag="U8116_001796"
     CDS             9990..10568
                     /locus_tag="U8116_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEN8434791.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10572..11093
                     /locus_tag="U8116_001797"
     CDS             10572..11093
                     /locus_tag="U8116_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN8434792.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11172..11726)
                     /locus_tag="U8116_001798"
     CDS             complement(11172..11726)
                     /locus_tag="U8116_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN8434793.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11979..12254
                     /gene="rpsP"
                     /locus_tag="U8116_001799"
     CDS             11979..12254
                     /gene="rpsP"
                     /locus_tag="U8116_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEN8434794.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12266..12517
                     /locus_tag="U8116_001800"
     CDS             12266..12517
                     /locus_tag="U8116_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEN8434795.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12642..13433
                     /locus_tag="U8116_001801"
     CDS             12642..13433
                     /locus_tag="U8116_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434796.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13603..14124
                     /gene="rimM"
                     /locus_tag="U8116_001802"
     CDS             13603..14124
                     /gene="rimM"
                     /locus_tag="U8116_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEN8434797.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14114..14875
                     /gene="trmD"
                     /locus_tag="U8116_001803"
     CDS             14114..14875
                     /gene="trmD"
                     /locus_tag="U8116_001803"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEN8434798.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15011..15358
                     /gene="rplS"
                     /locus_tag="U8116_001804"
     CDS             15011..15358
                     /gene="rplS"
                     /locus_tag="U8116_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEN8434799.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(15490..16251)
                     /locus_tag="U8116_001805"
     CDS             complement(15490..16251)
                     /locus_tag="U8116_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN8434800.1"
                     /translation="MIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKNPIDEVVIDY
                     KRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGEAIALSKDDV
                     HITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNP
                     NGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKLAELGTPLYA
                     IQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         5391 a         2936 c         3307 g         4704 t
ORIGIN      
        1 cttctctttg tacatatatg gtttgttttt atggatgccg agctttttta taagataagg
       61 ctaccgccca taacgaccca aggatttgtg gaactaagta aatacctgca agaagcaaga
      121 tcagctgggt cggttttaaa ggatgataga agtaacgcca ttcagtaaaa ttggcgcaaa
      181 ataaatagga agtgacagta gatataatat tggcaatgaa taaagatata atacgcctgt
      241 tcctttgaaa ataatatcca gcaaaaagag ataaaaatat caatgtgatg aacccgctta
      301 cggaatgata acgaaaatca agcagtaagg cgaaatgcac ataaggaaca aaagataaaa
      361 gcaacggaag gtttttccag caagttttta tcatctttca tcaccaccta acaagtgtta
      421 ttattttgtt agttttattg taaattgaat cctgcaataa tcaattactt tttcattatt
      481 tctagattaa atgaataaac tctcatgcca ttatctctaa aaaaaaagtt tttcccccta
      541 atttttttag atagaaaatt gtatagtatg atgagagaaa aagtagtttg ggggaacgtt
      601 aatgagaaga aaagcagtaa tcgatcggct ttatttattt ttttggtttt ttcctatcgt
      661 tttgctacct tttacatggg taaatgctgt tgtacaaggt gcggagatat taggcctgat
      721 ccaaggaagc ggatttgtgg tttctaggaa ttttccagct ttgactgttt tctatttagt
      781 gataagtgta ggtctcttat taccaaaatc tctgatcaat cactacaatt ggcagctgct
      841 ttacttttct tgcttattag tatttacatc tatgttttct ttgctgcctc gcttgattat
      901 cggccctaat ggtttgagcg agagtggatt gaaggattca gcttattcct ccatgtattt
      961 cttgacgatc aaaccatggt tctatttagt aattgcctca tttattcttg caacattttt
     1021 ttacttctgg acagaaatca ggataaaaag tgaacgatct gtatctataa aaaataacta
     1081 ttgatggtgg atcatcagta aaaaagcata tgagctgcgt aaaatgcatg tttctcatat
     1141 gcttttttta ttattatgta aagaatgcga aaaacgttta catgattttt acataactta
     1201 actatatttt tacagcaacc atacgaggga tttacagtcg actgataaac tttagttgta
     1261 aataagaaag gagcaataaa atgaaaaaag tattgtttac tctaggtatg atgggaattc
     1321 ttttagccgg atgcggctca ggatcaggaa atacagatgg ctctgccaca caaaataatt
     1381 caagtgactc aaatgaacaa gtaaaaatcg ttgcggtagg ttcgacagca ttacagccgt
     1441 tagtcgatgc agcacaagaa agctttgtac aagaaaatcc aaattatcaa atctctgtac
     1501 aaggaggagg aagcggaact ggtttgagcc aagtcgaagc tggagcagtt actatcggaa
     1561 actctgatgt atttgcagaa gaaagagacg gagttgatgc ttcaaaatta gtggatcata
     1621 aagtagcagt ggtcgggatg gcgccaatcg tcaataaaga tacagatgtc aaagatatca
     1681 ccaaacagga attgattgat atctttacag gaaaaatcac taactggaag gaagttggcg
     1741 ggaaagatca aaaaatcaac gtcgtgaacc gtgcaaacgg cagcggtacc cgcgcgacat
     1801 tcgaaaaatg gggactggat ggtgctaccc ctgtgcagtc ccaagaacaa gattcatcag
     1861 gaacagttcg gcagctggta agtcaaacac caggagcaat cagctattta gctttctcat
     1921 atctcgatga ttccacacaa gcactaagta tcgacggtgt agaaccaaaa gaagagaatg
     1981 tagcagataa cagctgggga atctggtcat atgaacatat gtacacgaac ggaaaacctt
     2041 cccctgaagt ccaaaaattc ttagactaca tgatgacaga agaaatccaa gaaggacctg
     2101 tcaaagaatt gggctattta ccaatcacaa tgatggaagt agaacgtgat cacgaaggaa
     2161 atatcaaata actagcagat aaacatgcca cggtagtcgt tactattaag gaaaaatcaa
     2221 ctatgtttcc cacatataca tttttccttt aattgtgaaa aatagttcct catattttgc
     2281 cgttttttga gctaataccg agggtcagct gattaaacac cgtttattcg aaaaaaagag
     2341 ctacgacata gaactgtcgt agctcttttt ttgctatatg acaggaatga aaggtgtttg
     2401 caagcttaat gtgaggaaca ctttccatat ctgttctata ctggatagga gaagtttgga
     2461 aatatccaga aaacgaaatc aatcgaggta gccacatgaa gaataaatgg ataaatcgaa
     2521 gtaaaaaaaa gaaagtaaca caaagatcat cagataaagt catccgtctt tttccatcgt
     2581 ataaagaagg attaacggca gatcaagtca aggaacgaat agaaaaaggt gcagcaaata
     2641 attcagtcga tcccacattc aaaacaaatc agcaaatcgt attggaaaac atatttacgt
     2701 atttcaattt gatctttctt atcttagcca ttttgctttg tttagtagaa tcctataaga
     2761 acttgacttt tttaccagtt atcatagcca atacaggaat cgccattttt caggaaattc
     2821 gatccaaaaa gatcttggac gaactaaatg ttttaaatac agcaaaagtc aatgtcatac
     2881 gggacgggat agaacaaaca atagatatag aagaccttgt aatagatgac attgttattt
     2941 tagagactgg acatcaaatt ccagcagatg cagaggtcgt ggaaggaaaa ctgcaagtta
     3001 atgaggcttt attgacaggc gaagcagatg aaataaccaa agaaataggc gatccattaa
     3061 tgtccggcag tttcattgtt tccggaaaag cttacgcccg attagacaaa gttggagcag
     3121 attcgtatat ttcccagctg acagtcaaag ccaagacaat gggagaagga gaacagtcgg
     3181 agatgattgc ttctcttaat caattgatca aatggattgg gatcatcatt attcctatcg
     3241 gtatcctgct tttttctcaa agttactttt ttaatggaaa cacgatcaaa gaaagtgtcg
     3301 ttgcaatgga agcagcattg atcggtatga tacctgaagg actgtacttg ctgacgacga
     3361 ttgcattagc attaagtgcg actaaactag caaagcaaag agttctttta cacaatatga
     3421 aaagcattga gacattggca agggtaaatg tcctttgtgt agataaaaca ggaacgatca
     3481 cagaaaataa aatgtctgtt caaaaagtga tcgtctctaa aaaacaagaa ataatcagtc
     3541 agtcagatca attggaagca tgcattgctg actatgcaaa agcgatggca ggagacaatg
     3601 caacaatgga agctgttaaa gcatatttta caaaaaccac tgatcaatcc tatcaccatg
     3661 ttattccttt ttcttctgtc caaaaattca gcagtgtagc gttggcagac aaggtgtatg
     3721 ttttaggagc gcccgaaatg gttttgcgcg atcaattgcc ggaatatgcg gaagagtttg
     3781 tttctttcgc tgaaaaaggc tatcgcgtgt tggtctttgg tgtgtacgat gggattcttg
     3841 aagaaccagt attgacagaa gcagtcacac cattaggcta tattctgatt gctaatccta
     3901 tcagaaaaga agcaagagcc acatttgatt acttcaaaaa acagcaagta gccatcaaag
     3961 tgatttctgg agataatccg ctcaccgtat ccaatgtagc tgtccaagca gggatttcac
     4021 atgctgatca gtacgttgat gcatctaaat tatcagaaga agaatatgaa gcagctgtcg
     4081 aaaaatatac agtttttgga cgcgtcaagc cagaacaaaa gaaaatcttc gttcaattga
     4141 taaagcgtaa caatacagta gctatgacag gtgatggcgt gaatgatatt ctagccatga
     4201 aagaagcaga ctgcagtata gcgatggctt ccggaaatga agccgccatg caggcctcac
     4261 aagtggtctt acttgattct gacttttcac gcatgccaga agttgttgca gaaggacggc
     4321 gcgttgtgaa caatattgag cgatcagcca gcttgttttt agtgaagaat attttctcct
     4381 ttttgctctc actgttttca gtgatctttg ctctgaccta tcctttagaa ccatcacaaa
     4441 tcacattgat tagtttgttt actatcggac ttccatcttt cttgcttgct ctagaagaaa
     4501 acaaaaagcg aatcagaggg aaattcatca tgaatgtgat ggaaaaagct gtgccaggtg
     4561 gactaacaga tatgatcgta gtcggtgcgt tggtgatctg cggggtgact ttgaatctaa
     4621 ataaaacaga tgtttctaca gcatctacca tgcttttgat tgcagtaggg ttcctcgttc
     4681 tctataaaat ctgttcaccg ctgaataaat tcagaagtca gatcattctt ttctgtgcta
     4741 gcggtaactt tttttcggtc atctttttgc ataagctgtt ttcaatcacc ggtatctcag
     4801 ctgtatcact tttgctgttg agcattctat ttttctcggc agattcgatt tttaggcatt
     4861 tgaccacatt ggtagaatat ttgtttgaac gtaaaaatga aaaagagcaa aagccgttta
     4921 gtcgcgtagc taaaaaattt ctatcgatat ttaaacgaga aaattgacga aaagtatctc
     4981 aaaaaagagt aaaaaagaac aaccactcgt gtatctttcg aagcaggatg agtcatccaa
     5041 ctttaaaacg cacgagcagt tgctcttttt tacattttcg gaatggtaac ggtgaaagtc
     5101 gaacctgttc caagatgact gtctacagta atcgtaccat taaggagttg cacgtagtct
     5161 tttacaatcg ctaaccctaa tccggtcccg cctgaatgtc ggcttcgcgc cttgtctacg
     5221 cggtaaaaac gctcaaagat ccgttcctga tcctcaatgt cgatacctat accaaagtct
     5281 tggacagaaa acgatagatc atctgttgcc tgatagcgga taataatctc gctgtcgctt
     5341 tttgaatatt ggacagcatt ttcaatcaga tttttgataa ttggataaag gatatctgtt
     5401 ttagtagtaa atggttcgtt ctttccaatc aatcgaatcg tcaaccgttt cttttcgatg
     5461 attggctgat aattttgaac gatttgctgg aaataaggtt ctgtataaat tgtctggatc
     5521 tcgtaagaat tctcgccatc ttttgacagc tggatgattt cccgaatcag tttatctaac
     5581 cggatcgcat ctttttgcat gatctcaaga aaagaagtca gagtttgcgg gtcatctttt
     5641 gcaccatcca gtaaagtctc ggtaaaacca atcagtgaag tcacaggagt ttttaattcg
     5701 tgagagacgt tgccaacgaa atccttttgt agcttttcta aacgtcgcac tttcgttaag
     5761 tcatacgcca cccctagaat ctgtccggta ccttctgaat tagtaaagta gcgcaaactc
     5821 aagtctaatg tctgccgacc ttctgtaagg gtgatctcct gatggatcag cggatgctca
     5881 ggagtcactt gatgaatcag ctgaatgagt tttggctctt gaatgacttc tgtgtaccgc
     5941 tgttcaggtt gatagcttac cacgtttaaa tgtagctgca tctttggatt aagcagtagt
     6001 aaacgagaat cagcatcatc aatcaggaat acgccaatca tcagttcgtt cagtaacgta
     6061 tataattgct cttctgtggt agtacacgcc cgatacatct tactcatttg ttcgctcaac
     6121 gcattaatcg tctgatacaa ttcttcccat tgatcgggtg tttgcatgat tacttccgcc
     6181 tgattaggat tttgcaccat tttttttagt acgggcagca cggttttcaa tggctggttt
     6241 ttttgataga tcagataata gatcattcct gcaagaacaa ggaagaaaga cagaaagaag
     6301 aaaaagaccc aacggcgaaa actctcagtt ctcggtaaaa aaccgcttgt cggttctgcg
     6361 atacgtaaaa tggcttctaa ctggccactt tttttgactg gtaatgcgac atataaaaga
     6421 tcctcattca aagtagtact tttgcgcaaa gcgctaccta ggttcccacc attcaatacg
     6481 gctttgattt cgggacggga gtttcgctgt tcattcaatg tttcattgtt tgtgtcaaat
     6541 aagatctttc cagtagcatc cattaaagta atccgttcgt tcgactgatg gacgaattct
     6601 tccaaagcag ttttgttttg cgctgcttgg aggttttcga catctaattg ccggatcagc
     6661 agttgtgctt tttttgtcag gtagctttct tgctgttcca cgatctgctg ttgaaaataa
     6721 tgggaaatca attgccagcc accaaaaaac aaactaacta aaagaataag aaagaagaga
     6781 cgttcataaa tttttttcca tttcatcgtt ttggttcctg aaacttatag ccaaatcctc
     6841 taatagtcac caagtacttt ggatgttttg gatctcgctc gattttttct cgcaaatgac
     6901 taacgtgtac atctacgatt cggctttgtc ctgcgaagtc aaaattccaa atgcggtcta
     6961 gcaatgtgtc tcggtcgatc acacggtctt ttcgtttcat gaaataaaca agtaattcaa
     7021 attctttcgg tgttagttca atcagatggt cgtctacggt cacttgataa ttggtcaaat
     7081 cagcagtgat ttgtccaatc gatagatatt caggctcagc ttcatctttt gattgcttac
     7141 gaggttcaat ccgtctgaat attgctttca tacgggctaa tacttcgcga ggactaaatg
     7201 gtttggtcaa atagtcgtca gcacctattt ctaggccaat gatccgatcc acttggtcat
     7261 ctttagccgt cagcatcaaa atcggtgttt catttttttc ttgacgcagc cgctgagtga
     7321 tctccatgcc atccatcgtt ggaagcatca catctaaaat gatgaaatca aatgattgtt
     7381 ctaatgctaa ttctaatcct tcgcctccat ctgtggcact ggttaccttg tagccttctt
     7441 tttccaaatt gaacgtcaag agcgttacaa tagaaggttc atcatcaaca actagtattt
     7501 ttttcattca aatctccttt aaataggttt cattctgttt atattttagc atagaatcag
     7561 tacaaagggc tattttacaa aagaaataaa tggtataata ggaaggcact ttgctgaata
     7621 gaaaggaaga gcagtcaatg attggaatca gtgcatgttt aggcggtctg gcctgtcgct
     7681 atgatggaca gaaaaaagaa gtaccagaat tgatggaact agttgaaact ggagaagctg
     7741 tcatggtctg tccagaagtg atcggcggat taccaattcc tagagatccc gctgaaatca
     7801 ttggtggaaa tggattcgat gtgtggaaag atcaagcaaa agtatggacg acaagcggcg
     7861 aagatgtaac ggaagcgtac aaaaatggcg cgatcaaagc ctatcaaaaa ttgcaggaaa
     7921 aacacattga tctagtcatc atgaaggaaa aaagcccttc atgcggaacc cattcgattt
     7981 atgacggaag tttttccgga ataaaaaaaa atggtgccgg agtggctact gcttatttta
     8041 ttcagcaaaa aatgactgtg ctctctgatg aagaatggct gaaaactaga ggtgagcaga
     8101 atggaaatcg aaaagaccaa tcgaatgaac gcgctctttg aattttattc gaccttattg
     8161 acagaaaaac aaatgaacta catggaatta tattatgcag atgatttttc attaggtgag
     8221 atcgcggaag aatacgaagt aagccgacaa gcagtatatg acaatatcaa gcggacaagc
     8281 aagatcttgg aggattatga aaaaaaactc catctttttt ctaattatat tgttcgagaa
     8341 caggtattgg aaaatatgac tgcatatatt acagataagt acccggaaga taaaaaacta
     8401 cttgaatatg tacagcaaat tcaagcaatc gaagaataaa taaaaggaat gaagaaaata
     8461 tggcatttga aagcttaact gaacgtctcc agcaggcgat gggaaaaata agaaaaaaag
     8521 gaaaagtatc agaagctgac gtaaaagaaa tgatgcggga aatccgcctt gccttactag
     8581 aagccgatgt taacttgcaa gtagtcaaag attttactaa gcgcgtacgc gaacgtgcgg
     8641 taggggcaga agtgctggaa agtttatcac ctgcacaaca aatcgtaaaa atcgttgatg
     8701 aagaattgac gatcacactt ggttctgaaa cagcagagtt aaataaatca ccaaaaatac
     8761 caactgttat catgatggct ggtcttcaag gggcaggtaa aacgaccttt actggtaaat
     8821 tagccaatta tttaaagaaa aatgaaaatg ctcgtccgct cctaatcgca ggggacgtat
     8881 atcgaccagc tgcgatcgac caattgaaag tattaggtca acagctagac gttcctgtat
     8941 ttgatatggg aactgaagtc agcccggtag agatcgtccg tcaagggatg gagttagcaa
     9001 aagaaaagaa aaacgactat gtattgatcg atacggcagg acgtctgcat atcgatgaaa
     9061 cattgatgga tgagttgaaa caaatcaaag aattgacgca accaaatgag attttgcttg
     9121 ttgtcgatgc catgactggt caagatgccg tcaacgtagc tgatagcttc aaccagcaat
     9181 taggtattac aggggtcgtc atcactaaac ttgatgggga tactcgaggc ggggcagcac
     9241 tatcgatccg ctcagtgaca ggcgcaccaa tcaaattcat cggttctggt gaaaaattga
     9301 ctgacttaga agtattccac ccagatcgta tggctagccg tatccttggt atgggggata
     9361 tgctgacatt gattgaaaaa gcgcagcaag actacgacga gaaaaaagcg gaagaacttg
     9421 cacagaaaat gcgggaaaat tcattcgatt tcaatgactt tattgaacag cttgatcaag
     9481 tgatgggtat gggcccactg gaagatctaa tcaaaatgat cccaggaatg aatcaagtcc
     9541 ctggaatcga aaacgtcaag gtagatccaa aagatgttga acgcaaaaaa gcgatggttt
     9601 attctatgac gccagcagaa agagcaaatc ctgatctgtt gaacccaagc cgtcgtcgcc
     9661 gaattgctgc cggttctgga aacagtgttg ttgaagtgaa tcgaatgatc aaacaattca
     9721 aggaatcaag aaaaatgatg cagcaaatgt ccaaagggga tatgaacatc ccaggtatgg
     9781 accaaatgtt tggttcaggc gtcaaaggaa aactaggcaa aatggcgatg aatcgaatga
     9841 tcaagaaaaa taagaagaaa aagaaaaaac ggaaataaga tgaaaaaaac agcttcggta
     9901 cagactgaaa atgtctactg ccggagcttt tctatcagca atttttattc tttactaata
     9961 caattactct aaacgaagag gtggcacaga tgagatgtcc gtggagtgaa caaacagaaa
    10021 gtatgcggca atatcatgat actgtctggg gtgttcctga atatgatgac caaaagctgt
    10081 tccgaaaatt gatgcttgat attaatcaag ccggattgag ctggcagacg attttaaata
    10141 aaagtgaagc attcgatgaa gcatatgatc gattcgatat caaaaaagtc gcaaattatg
    10201 gagaaacaaa aatcagtgaa ctaatggaaa acaaaggaat tattcgtaac cgaagaaaaa
    10261 tcgaagcagc cgtccaaaac gccaaagcag tcttgaaaat tcaagaagaa atcggcagtt
    10321 tttcagagta tctttggtcg ttttctaatg gaaaaatcgt tgattcttct tatcattcac
    10381 aagaagaagt cccaacaaca agtcctcttt cagatgaatt agcaaaagat ctaaagaaaa
    10441 gaggatttaa atttatcgga agcacaacaa tctatgcttt tttagaagct gtcgggatca
    10501 tcaatgatca ccttgacagt tgtttccgca aagaagaggt cgtacgtatc ggcagaaagg
    10561 gcgaataaag catgcgaaaa atcatatttt atggtgctat aagtttagat ggctatttgg
    10621 caacgaaaac ggatgatctt caatggcttt ttgacactcc aacaggtaag caaactactt
    10681 atgaagcttt ttatgaaaca atcgatacga ccatcatggg cagaaaaact tatcaggaag
    10741 caaaaaaata tttggagacc gaagcaattt atccagagaa aaagaatttc gttttttcca
    10801 cccacgcaca attacaattg ccagatgctg aggtcgtaaa cgaagatcct gttgcttttc
    10861 taaaaaatct acaaatgaca gaaggaaaag atatctgggt tgtgggtggc ggtcgattga
    10921 tcaaaccgct attggaacat caaatgattg atgaatggta tatccagatt acccctgtat
    10981 tattagggga cgggattcct ttattccaaa aaggaacata tgaagcgcgt tttgaaatgc
    11041 tagacacgac acgattcggt gaatttatcg aattgcatta tgtacgaaag tagaagtaaa
    11101 taaaataaaa aaggcaacta tatgcagaaa tgtcctgtac atagttgcct tttttattac
    11161 cgttatgctt tttactctgt taatttgtta gaaaaatcta gatacgcatc taaaatcgtt
    11221 tggaagaaac ttaatgttcc ttcaacgata ttaccatttt catcaattag attcgcaacg
    11281 tttccgatat atgcttccgg ttgctgcaag gttggcacat tcaaaaagac aagtgattgg
    11341 cgtagatggt ggtttgcgcc aaagccgccg atcccacctg gtgatgcagt aacgactaaa
    11401 cctggtttat gatcccaaac actgtgtcca taaggtcttg agcctacatc taaagcattt
    11461 ttcaatactg ctggcacaga acggttgtat tcaggagtaa cgaaaacgac accgtctagt
    11521 cccctcactt cctcacgaaa acgagtccat tcagcaggag cctgttctgg agtttcgaga
    11581 tcctcattgt aaaatggcag atcatcgatt tttacaaata cactttcgta cccttcagga
    11641 agcaatttcg caaaagcttc tgctactttt ttgttgtaag aatcttttct taaactacca
    11701 ataaaaaatc caattttttt tgtcatacga tttttcctcc ttcaaactat ctataataat
    11761 cttacaaaag ataagtaact ggagcaaata aaaacactta ctgacaaaag ctacatagaa
    11821 gatagattac ttatttttaa caaaaaagaa gtctaaaatc ttctattttc taaaaaaaat
    11881 cgaatttaat caagctgtaa agaaaaaaag ctttacaggg taaaaaaaaa ctgttacact
    11941 acatcttgtg aataaaacaa tggaggtgga aataaataat ggcagttaaa atccgtttaa
    12001 aacgtatggg ttctaaaaag agtccgtttt accgtatcgt cgtagctgat tcacgttctc
    12061 ctcgtgatgg acgtttcatc gaaactgtag gtacttacaa tcctttgaaa gaccctgcag
    12121 aagtagtttt aaaagaagac ttagttcttg actggttatc aaaaggtgca caaccttctg
    12181 acactgtacg taacatcctt tcaaaagaag gcgttatgca aaaacaccac gaagctaaat
    12241 tctcaaagaa ataaggtgac cggatatgaa agatttaagc gacttagtct taacaatcgt
    12301 tcgtccgcta gtcacttatc ctgatcaagt caagttagac gtcgtagagt cgaaagactt
    12361 ttacgagtac aatctaacag tggctcctga agatattggc cgtatcattg gaaaacaagg
    12421 gcgtgttgct aaagcaatcc gcacaatcgt ttacggtgtt cggatcaatg caccgaaaaa
    12481 agtgcgtttg aatatcatcg atgataagga aaagtaaata aacgtgctgt actcgaattt
    12541 tcgagtgcag cttttttgtt ttttttatta attgaagaaa tgattatttg gcaaaacaag
    12601 caagaaagcg tatccttata ggaggaggga gtgaacagcc aatgagaaag aaaatgttat
    12661 ttattccatt tgctctattg ttaagcgctt gtagttcggt tactcaagaa tcagacaata
    12721 cacatctatc aagcagtata tctgaaactg ttaccagtcc agaaaaaagt agtaccaaaa
    12781 cggcttccac cactgaacaa acaatggcta cctcaaataa tgagaaaaag actgctttag
    12841 accagttgaa agaacaacag ccaaatgttc ccatgcctct agatgtacca gtctcttcag
    12901 gctatctcaa tatcgcagcg acacatacaa agcaaggtta ttctatttta tattacagaa
    12961 cagatcgtcc cctcgggttg aatgctgacg aattgaatca agaaacgcct attgcaacct
    13021 atctatatca atatgggttt gcttctagtc aagaaacaat ccaagttttg cagccatttg
    13081 agatcgacac aaatggtcag caagtggact taggttctcg aatcacaggg tatcagcaag
    13141 gtgcagcagg ttcaagtttt cttgaatggc aagaaggaaa ctggcgtatt cgaatccgtg
    13201 ggaataatat agaaggccaa gatcccttac tattagctaa agaaatcgta gcttatttgg
    13261 aagaaaacag tctaccagca cctgaacaat ttggtaaaat tactgttgat atgggtgata
    13321 cgacgaatcg agccgtagaa gtaagctggc aagaaccgaa aaatgcctat acgatcactc
    13381 atcaagatcc tatgtctgcg ttgaagatgg cagtttctat gaagaggttg tgaaatcagc
    13441 gcgctgatct gagcccatca tttaactcca agtcacagga agtaatcaaa aatagcacgc
    13501 cgcatttttg cataattttg gcttaatacc ttaaaagaaa actagaattg cctcttcaca
    13561 catgctaaaa taaaacagac aaaaaagaaa tggggatata agttgactga atacttgaat
    13621 gtaggaaaaa tcgtaaacac acaaggtatc aaaggcgaag tccgtgttat ctcaacaacg
    13681 gatttcccag aagaacgtta caaaaaagga acggtgctaa cactattcca agacggaaaa
    13741 gcacctgtgg aattaacagt caaaagtcat cgcaaacata aaaattttga cttattgagt
    13801 tttgaaggac atccttctat caatgatgtg gaaaaatatc gggatggcat attaaaagtg
    13861 tcaaaagaca actctgtaga tctagctgaa aatgaattct attaccatga gatcatcggt
    13921 ctgcatgtat tagaagatgg aactgagtta ggtaaagtaa aagaaatctt gtcaccaggt
    13981 gccaatgatg tttgggtcgt tcaacgtccg aaaaaagctg atatattgct tccatatatc
    14041 gcttctgtcg tcaaagaagt cgatttagaa gctggagtgg tccgtgtaga aattccagaa
    14101 ggactgattg acgatgaaga ttgatgtatt gactttattt ccaagaatgt ttgaaggtcc
    14161 tatgggggaa tccatcatcg gtaaggctgt agataaagga ttattagata tcaatatctc
    14221 taacttccgc gattattcag ataacaaaca tcagacagta gatgattatc catacggcgg
    14281 tggagcaggt atgctgctca aagtccagcc catctatgat aacatccaag ccattgaaaa
    14341 agctgcaccg gatgtcaaaa aaagagtaat tcttcttgac ccagctggta aacgattcga
    14401 tcagaaaatg gcagaagaat tttcacaaga agaacactta gtattcattt gtggtcatta
    14461 tgagggatat gatgaacgta tccgcacatt agtgacagat gaagtttcct taggagatta
    14521 tgtccttact gggggagaat taggtgcgat ggtcatgatc gatgcaacag ttcggctttt
    14581 gccagatgtg ttagggaatc agacttcagc tcaaacggat tcttattcaa ccggtttatt
    14641 ggaacatccc caatatactc gcccggcaga atataaaggg atgaaagtac cagaagtatt
    14701 gacaaatgga aatcataaat tgattgaaga atggcaatta aaagaatcac taagaaggac
    14761 ctatcaacgt cggcctgatc ttttggaaac acttgaatgg acacctcaaa tgacgaaatt
    14821 tttagaagaa atcaagaaag aagaacaaga aaaaacagca gatgaagcca agtgactttt
    14881 tctaaaatct tctttacagt aggttgataa tatgttagta ttttatggtg agtgcaaaag
    14941 cacttaacta ttacgatatt ccgctgtgag aaaagctcat aagaatattt ggaataagga
    15001 gaagaaaacc atgaatccat taatcgaaga attaacaaaa gaacaattac gttctgacat
    15061 cccagcgttc cgccctggtg acactgtgcg tgttcacgca aaagttgttg aaggtactcg
    15121 tgaacgtatc cagttatttg aaggtgttgt tatcaaacgc cgcggtgctg gaatcagcga
    15181 aacttataca gtacgtaaag tttctaacgg agtgggcgtt gaacgtacat tcccattgca
    15241 tacaccacgt gttgcaaaaa tcgaagtagt tcgctacggt aaagtacgtc gtgcaaaatt
    15301 gtactaccta cgtgcattac acggaaaagc tgctcgtatc aaagaaatcc gtcgttaatc
    15361 atcgttatca gacagagaaa gaacccttgg tattcaaggg ttcttttttt attatctgtt
    15421 tttttatatt aacgatttct atttggtaca tttttgatta ataaaaaggg gcaaaaaagg
    15481 ggcaacaagt tacagttttt ctatatcttc tttcaatttt tcttttactc cttttgtcac
    15541 atgcaaataa attttttcag tgatatcact attttcgtga ccaacgcgat cctgaatggc
    15601 atacagagga gttcctagtt ccgctaattt tgaaatatgg gtatgtctaa aaatatgtga
    15661 actaagtttt ttatcaatct tcatgtcagc tttatgactt ctcaaatagg tgttaattgc
    15721 tgttagttgg aaaggagttc ctttcgttgt ttgaaataga aattgaccat ttggatttaa
    15781 ttctagaagc tcattgtaaa tagctatcgc tttttttggt aaatcaattt ctcgcattcc
    15841 ggcagctgtt tttgtagaat cagatttttt catatcagct attgaacgtt ctctatacat
    15901 catcgtccca tttataacta ctgatgcatt attattggta atgtgtacat catctttact
    15961 taatgcgatt gcttcaccag acctcatccc agtcaaatat agccactgga aaaggagaga
    16021 atatctttta ttgtgtgaag ttgtgaaatc tactagtcta ttatattcat catcttctaa
    16081 aaatttatct ttaattttta tcgtttttga ctctctttta taatcaataa ctacttcatc
    16141 gataggattt ttttctacat aacctttttt catagcatat gaaaaaagaa gattcaattt
    16201 ggatttaatt acactgacgt acttatttga caagtcatct ttatatatca tgtcttcaaa
    16261 aatattattc aaatctattg tattaatacc agaaacgatg taagtctcag gtatcttctt
    16321 ctttatagtg tttaatat
//
LOCUS       DASNNU010000057        16083 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.057, whole genome shotgun sequence.
ACCESSION   DASNNU010000057 DASNNU010000000
VERSION     DASNNU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.057"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            72..1181
                     /locus_tag="U8116_001806"
     CDS             72..1181
                     /locus_tag="U8116_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEN8434801.1"
                     /translation="MKSKIIKLLAGKASKKEKIQILLAFLGSSTAVFTALLIVVAGLL
                     LVIFIGGASQTTSTEIGGGGISVSEMTMQWKDKVTKEAEKNGIPDMVDPLLGIIEAET
                     GGNAVRYPDVMQSSESQGNAPNTISDPYTSIEVGVKYFAGLVKAHKDMDVLNIIQSYN
                     FGPGFLSFATNNYDLNRAIAFANKYAHGVQVTYTNPVAVNLGYTYRYAYGNMFYAMIV
                     KQYLTPKSSGLPGGSSDMAKVAQAEIDEGLHVGGQKYWSWYGYGGRVEWCAVFVSYVA
                     AKANVNMEKFAYCPTGIQNFKQKNEWQPVGVEPKDGWVIFFDWDADGISDHVGIVKSY
                     SNGTVYTLEGNSGDSTKERSYGKTSQYIMGYGIPK"
     gene            1206..1862
                     /locus_tag="U8116_001807"
     CDS             1206..1862
                     /locus_tag="U8116_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434802.1"
                     /translation="MIKEAFKQWKHALKEEHLQIKIQNKWFEELKKTDFYRQLNKYKW
                     LRNSFILIDYIILVGCYLSIYTLPASELNALFGNQISQDYYQIIVFNYTIFILILDLF
                     IVLFLSNYEFKTNRIFTNIMIIGLFGGLNTFLLAFSFVVQPAYLLFVLGLVIVKVVIR
                     KWKELQREAVFLSYKEAEKEDLINHYNRHGIRFEMEDEPRIYRMNYFQEENKNKELRS
                     "
     gene            2219..2455
                     /locus_tag="U8116_001808"
     CDS             2219..2455
                     /locus_tag="U8116_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434803.1"
                     /translation="MTQSIGDMDNEDAKMSVEDLRVYTNDDQTYFVVFDLKYEGLDSN
                     QVLVYVASAHVSDSNQIDHYELIATNTKLKKGNE"
     gene            2452..3333
                     /locus_tag="U8116_001809"
     CDS             2452..3333
                     /locus_tag="U8116_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016612340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434804.1"
                     /translation="MMVIFGKISHFISSVKEMKSKIKHKHFRTKTQTYTKILMAFGFL
                     FLSIIGQAVLSKPANAYSESSVEKSIPFDAGEITVLKKQYSPVSKSYRMDIYVDSEVD
                     LVSDGEPVTKATAVTKEDINTPLKVEVIPVSENYFVLLAQNIDNEFGALRFDVTYKRS
                     KDSDESALSLYGSQENSTLVKSDLSTLSQAELKISGLENDIRLQQLNRKMIEEENKSL
                     IEEIKNTREKIASFTEEKEYKVGEQLAKTEDSITSLENTIESFKGNISDNQTKISDIE
                     KTIQLIESKIDELKKGV"
     gene            3340..3777
                     /locus_tag="U8116_001810"
     CDS             3340..3777
                     /locus_tag="U8116_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434805.1"
                     /translation="MSKKRIPVFLDTEHEDALKELKIYLPVDEDIKDSPMIRYALLEL
                     LNRVRKEINFEKKVLDSLSLLEIMTNAKLEQDHTKIQDLFSSDVYQEAVTMSKKVVRN
                     NNYRFKNRKDESKKEEKKKVKSVQEDVKKERDFNDIVSQYLPK"
     gene            4031..4303
                     /locus_tag="U8116_001811"
     CDS             4031..4303
                     /locus_tag="U8116_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434806.1"
                     /translation="MPRFTLIIENEPLVAYLEQRAKKQTFNTGKKVTRNEVINQILQN
                     EMINDLTNNREVDAIKDSLDDFKHILQQYVDTNNALLYRAFESDGI"
     gene            4321..5832
                     /locus_tag="U8116_001812"
     CDS             4321..5832
                     /locus_tag="U8116_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016612337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434807.1"
                     /translation="MAQSAGSYINLMSGFVISFDSEYVQYMDREDKKVYQEKDLSDFE
                     TAMTNDDPSQSFEGYLDYMDRKNALLAQSKLTAEERVSLGEINKRLADYSTLSKVQIE
                     IMQKETQEKIWSLNTGMFDFNNDDLTQNEVEQYKELFDRANKNESVLFQDVVSFDTKA
                     LIEAGIYNPYSNELKREPLIMAGRKMMNVLYEKEKLNGSTISVGEIHYNTGHFHVHFA
                     TTEVEPTREMVFVKEHNRYERKGQRSDETLEKMKSTFANLIFDRSSELERLNDLRNGL
                     RNEIKTEMATPKKESVLLLEKLKAQLPENQSKWNSKNLTPESRETMNQLIDSLMKDNG
                     HFEEFKQLAKEEDIFKKATYGEGDPNKEKKSFYDGRMYGPDGIYYRLGNSILQELKKE
                     NDSSQQDKPFHRSSVSGQQNPNKETYAKLKHDYFMRKDTNESRSPPKRNPSERTKPFK
                     ESFKYRRSVTNQVKHLERVTSNEFDQFKNKVAFEKLQREIEQAKMSQESDLFN"
     gene            5849..6040
                     /locus_tag="U8116_001813"
     CDS             5849..6040
                     /locus_tag="U8116_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434808.1"
                     /translation="MENYYFINSVDPENQTMRIQEVGAGEMSAQQVRITKEDADVYSL
                     MLDEATQEGEPLIVQLDLK"
     gene            6606..9638
                     /locus_tag="U8116_001814"
     CDS             6606..9638
                     /locus_tag="U8116_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HEN8434809.1"
                     /translation="MEQQDNNRQETDNSRLARAKRKLGRLKREYSAQISRVFDHQAQT
                     NGQPMNDKENGRSFFNRQEQLEGRARTFLREIEAQEERISMLEHQKERKELGLNNQGG
                     LIMSVDNIPMIKAEIEQAKQGNSMYSAATIRKYEKQLVELEELKRRSERADKHMNEQA
                     QKMIESGRLTQWKKNPMIYFVKGYRKLAVELTESGTFEPSQRYAAKTEAEQSVISDIL
                     SELKFNTEEIPNATKDQDAALNHFYRVEFNEGSESIRNFEGEIVSQPLIEEIKRLDRP
                     MESGYYKFYFQEIKDGEIVDAFRLDVGDGVNSTQDYYKKLESLAAQDKTNAVTNERPK
                     EAVKSGKNDAVKKRKSADELKKETDQLVKDAMENIKNHFDDPEDVLELGAFMSKFYNY
                     SLKNTALIQNQLPHAHAVAGAKKFKEAGFFIRKGEKAIRVLAPTTYKCFINEQGRSQS
                     LSKATKLQKEKILKGELKTEERRAFKNVPVFDISQTTATADDLPKIFPNRPYNFDLTK
                     ATNTKLLYQSLETYAISEGLTIIDDLTTVTPVLGTAKGAYGQGIDGSEMLFMNPRFTQ
                     AEQVPTMIHELAHAKLHSKTSNSAYSSSVKEFQAELAAYIVAHHYGIDTKEESIPYIA
                     AWTKNGHTVEDKLAQMEEVKVFSRQMIEQIDTQLVIQQELEQKEQEQAPTVEKQKLTE
                     SQKTYKTFSEEIAFAKTVSILDIAHKNGIDLKKDSAKQYRIADNHSVVITAPKNLFIE
                     NNGHFGGGPIDFVQKIVGIKDFKEAVRHITDNDYDATEFVPEEKIDYKYDKENERPFV
                     RAKKYLIEERCIDEELVNVLHNKGFLRQDKRNNLVLIWNNQGEICGNSEKGTIKDSTW
                     RKIEPGSDNFVGFNVRNGEPKRLKFFESGVDLMSYMTLNKSDLQDTWFVSMEGLKPET
                     VYYYLEESQRVAPSGVEEISFCVDNDNSGKEFLERFKKFTYNQKEKQPIEFQAEFPQA
                     PVGQETQKWDWNNELVYQVNEGENQRKKSLESEPALSL"
     gene            9652..10065
                     /locus_tag="U8116_001815"
     CDS             9652..10065
                     /locus_tag="U8116_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN8434810.1"
                     /translation="MKRMIFTIIQGIGMAVVLSLLSMWIGEQFFSTYLPRLPEARHGI
                     SISAATFSIVLAPIIEEYLIRVKLLPFLIRRTNLPAAVLFSSFIFSVLHGQVFLLPYF
                     LNSLVYSWVSLKTKKAYGSMIVHSAYNFIALIPGL"
     gene            10127..12307
                     /locus_tag="U8116_001816"
     CDS             10127..12307
                     /locus_tag="U8116_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEN8434811.1"
                     /translation="MTTVILAEKPSQAASYAGALKHSVKKDGFFEIQDPIFSDETFIT
                     FGFGHLVELAEPGHYDEKWKSWALDALPIFPQQYDFQVAADKKKQFKIVSGLLKKADT
                     IIIATDSDREGENIAWSIIHESGAFSTKKTYKRLWINSLEKDVIRSGFQNLKPGMDYY
                     PFYQEAQTRQIADWLIGMNGSMLYTLNLRLKGANGTFSLGRVQTPTLYMIYQRQQLIE
                     HFKKEPFYEIESLIKATNGSFKALLSPSQRFSTKEELLTFVSSKGAAIGTQPGTIKDL
                     QTKMKKTNSPNLFSLSSLQSKINQLYKATASQTLKAMQGLYEAKLLSYPRTDTPFITD
                     SEFQYLKANYDSYARFLSIDEPMAQDQPRKRYVDGSKVQEHYAIIPTKQVASESAFNK
                     LDDLQKKIYLLVMKTTIAMFLPDYTYEETVIETMVSTLCFKATGKVPKVEGWKILFKA
                     DTNKENEESQTLPVVMIGEAIQAEVKSVQKETQPPKPFTEGTLLTAMKTANKTVDDEQ
                     AIKILQEVEGIGTEATRASIIETLKQKEYIKVEKNKLIVTEKGKLLCQAVESQHLLTS
                     AEMTAKWETYLKKIGKKEGNQETFIANIKKFIVHLIEHVPDDVQKLNFHTYQAEVKKE
                     TDKQTIGKCPKCGGNVVSKKSFYGCSNYPTCTFTLSDQFRKKKLAKTNLKDLLVGKET
                     VVSGIKKADKTTYNAKIKLSDTGQITFVSFVNAPKKAKKVGNKA"
     gene            12304..12758
                     /gene="ssb"
                     /locus_tag="U8116_001817"
                     /pseudo
     CDS             12304..12758
                     /gene="ssb"
                     /locus_tag="U8116_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            12864..13169
                     /locus_tag="U8116_001818"
                     /pseudo
     CDS             12864..13169
                     /locus_tag="U8116_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13371..13514
                     /locus_tag="U8116_001819"
     CDS             13371..13514
                     /locus_tag="U8116_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEN8434812.1"
                     /translation="MKVSVHSLERRSLLSVAEALGLMISFGSLIATIIFGILTAVKND
                     KKK"
     gene            complement(13746..14006)
                     /locus_tag="U8116_001820"
     CDS             complement(13746..14006)
                     /locus_tag="U8116_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434813.1"
                     /translation="MFSKFKEKSNAKKEQKKHLQRKKNFELRQAGKDPQKGWHSMVDT
                     GMSGSCMRGAAVIDITGENLETYNYFSENQKQLAKEIDKKNQ"
     gene            14297..14542
                     /locus_tag="U8116_001821"
     CDS             14297..14542
                     /locus_tag="U8116_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434814.1"
                     /translation="MTEERKCYGGQWKVPITPYNRRLYWPPSWIECDCGELAKQVYVK
                     KGDFFYPNGHYLCKTCQREYRKVDGRDQFILIKPNEE"
     gene            14724..15089
                     /locus_tag="U8116_001822"
     CDS             14724..15089
                     /locus_tag="U8116_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN8434815.1"
                     /translation="MLELNKSEYQILYYFYEFNQPLTKHELLERIPDLNKNTAAVVIK
                     RLLDKGYLEVGEIKYSNTVLARAYRPCLPFIEFFKDEYGEFAVEKLVKYAISSLEDKE
                     KLTYLSNLIAEKKSLHTIE"
     gene            complement(15482..16057)
                     /locus_tag="U8116_001823"
     CDS             complement(15482..16057)
                     /locus_tag="U8116_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434816.1"
                     /translation="MKKTSLSILTLLALVVIGGCTSENNDNGTTSSSIEVSVTSDSAT
                     NLTGDTVDIVRTEGNNTNFKVEQKEGQSVHIVSTNIKTEEATEIVNSEFVNDSQSREL
                     ELIFTSQNNKFEVSLSVSGSEELSDTSTFTADQKYVEFIPYTSEEKNKLGVFVMSNDQ
                     NVITEIKNDLDNIDKYLDSDSDFYEQSIEVK"
BASE COUNT         5678 a         2745 c         3236 g         4424 t
ORIGIN      
        1 cagtaactac aagatcaaaa cggatcctga tcaagaacag ttagtcagat ttgcaggagg
       61 tcactaataa aatgaaatcg aaaataatta aattattggc tggaaaagca agtaagaagg
      121 agaaaatcca aatcttactt gcttttttag gtagttcaac cgctgttttt actgctctac
      181 ttattgtggt agccggattg cttcttgtga tctttatcgg tggtgcttca caaacaacgt
      241 ctactgaaat aggtggtggt ggaataagtg tctcggaaat gacgatgcaa tggaaggata
      301 aagtcacaaa agaagcagag aagaacggga tccctgatat ggtggatccc ttacttggca
      361 ttattgaagc agaaacgggt ggaaatgccg tacgttatcc tgatgtcatg cagtccagtg
      421 agtcacaagg aaatgcaccg aatacgatct cagatcctta cacgagtatt gaggtcggtg
      481 tgaagtattt cgctggtttg gttaaagcac ataaggatat ggacgtgtta aatatcattc
      541 agtcctataa ttttggccct ggattcttgt cctttgcgac aaacaattac gacttaaatc
      601 gagcgatcgc ttttgcgaac aagtacgctc acggcgtaca ggtgacctat acgaatccgg
      661 tggcagtaaa ccttggttat acctatcgat acgcctatgg aaatatgttt tatgccatga
      721 tcgttaaaca atatctaacg ccgaaaagtt ccggtcttcc tggtggaagc agtgatatgg
      781 cgaaagtcgc tcaagcagaa atcgacgaag gtttgcatgt tggaggacaa aaatattggt
      841 catggtatgg ctatggtggt cgtgttgaat ggtgtgcagt ctttgtttcc tatgtggccg
      901 caaaagcaaa tgtgaacatg gaaaaattcg cctactgtcc cactggtatt caaaatttca
      961 aacaaaaaaa tgaatggcag ccagtcggtg tggaaccaaa agatgggtgg gtgatcttct
     1021 ttgattggga cgcagatggc atcagtgacc atgtgggcat cgtaaaaagc tactcaaacg
     1081 gcacggtcta tacgcttgaa ggaaactcag gagacagcac gaaagaacgc agttatggca
     1141 agaccagtca gtatattatg ggctatggca tacctaaata agtcatagca aaggagtgaa
     1201 acaacatgat caaagaagct tttaaacagt ggaaacatgc gctgaaagaa gagcatttac
     1261 aaatcaaaat acaaaataag tggttcgaag agctaaaaaa aacagacttt tatcgacaac
     1321 ttaataagta caaatggctg aggaatagtt ttattttgat cgactatatc attttagtcg
     1381 ggtgctatct atctatttac acgttgccag ctagtgagct aaacgctctt tttggtaatc
     1441 aaatttcaca agattattac caaattatcg tatttaacta tacgattttc attttaattt
     1501 tagatctatt tatcgtttta tttctatcaa attatgagtt caaaaccaat cgtatattta
     1561 caaatattat gataatcggt ttatttggag gattaaatac cttcttgctg gccttctctt
     1621 ttgtagttca accagcatac ttattgttcg ttttaggact agttattgta aaagtagtca
     1681 ttcgaaaatg gaaagaacta caaagagaag ccgtcttcct atcttataaa gaagcagaga
     1741 aagaagattt gatcaatcat tataatcgtc acggcatacg atttgaaatg gaagatgagc
     1801 cacgtattta tcgaatgaac tatttccaag aagaaaacaa aaacaaggag ttgagatcat
     1861 gagttatcaa cgattgagcg gtattttaat acttggttta ttagctagct ggggagtttt
     1921 tgggctcttt atctggcatt caagcaaaca accagaagct acttcagaaa ccaaaattga
     1981 acaatctatt gaagagaatc agttggattc taacaatagc agcactgttg atcctacaga
     2041 aaatcaggta tcagaaaccg ttgaaagtga acaaaaaaat caatcgacgg atgaaaaaag
     2101 taccgttgtg caagattttg tgacgaccta tttcaacaca aaagaagagc catttcttaa
     2161 agatcgtgtc tcgctgctga acgaaaagaa attgattact gacgaatata accaagaaat
     2221 gacgcaatcg attggggata tggacaatga agatgccaaa atgagcgtag aagatctacg
     2281 tgtttatacg aacgatgatc aaacctattt cgtcgttttt gatctcaagt atgaaggatt
     2341 ggattcaaat caagtccttg tttatgtagc atctgcgcac gtcagtgact ctaatcaaat
     2401 cgatcattat gagctgatcg caacaaatac caaattaaaa aaggggaatg aatgatggtt
     2461 attttcggaa aaatctctca ctttatttct tcagtaaagg aaatgaaatc gaaaataaaa
     2521 cataaacact ttcgaacgaa gacacaaaca tacaccaaaa tattaatggc ttttggtttt
     2581 ttatttctat cgatcatcgg tcaagctgtg ttatcaaaac cagcaaatgc gtattcggaa
     2641 tcgtcagttg aaaagtcgat tccttttgat gcaggagaga tcacagtact aaaaaaacaa
     2701 tattcacctg ttagtaaaag ttatcgaatg gatatttatg tcgattcaga agtcgatctt
     2761 gtatctgatg gagagccagt tacaaaggca actgccgtca caaaagaaga tatcaataca
     2821 ccattaaaag tagaagtgat tcccgtttct gaaaattatt ttgttctttt agcacaaaat
     2881 atcgacaatg aattcggcgc tttgcggttt gatgttacgt acaaacgatc caaagattcc
     2941 gatgaatcag cgcttagtct gtatggatct caagaaaatt cgacgctcgt taaaagtgat
     3001 ctttcaactt taagtcaagc tgaattgaaa atcagcggac ttgaaaatga tatccgatta
     3061 cagcagttaa acagaaaaat gattgaggaa gagaacaaat ctcttatcga agaaatcaaa
     3121 aatacacgag aaaaaattgc ttctttcact gaagaaaaag aatataaagt cggagagcag
     3181 ttagcaaaaa cggaagattc gatcaccagt ttggagaata cgattgaatc atttaaaggg
     3241 aacatttcag ataatcaaac caaaatttct gatatcgaaa agacgattca gttgattgaa
     3301 tcgaaaattg acgaactaaa aaaaggtgtg tgagatagca tgtcaaagaa acgaattccg
     3361 gtatttttag acacggaaca tgaagatgct ttgaaagaat taaaaatcta tctgcccgtt
     3421 gacgaggata ttaaagacag tccgatgatt cgatatgcgc tgcttgaatt acttaatcgg
     3481 gtgaggaaag aaataaattt tgaaaaaaaa gtattggatt ctttgtcttt attagagatt
     3541 atgaccaatg caaaattgga acaagatcat accaaaatac aagacctttt ttcttcagat
     3601 gtttatcaag aagcagtcac aatgagtaag aaagttgttc gaaataacaa ctatcgcttt
     3661 aaaaatagga aggacgaatc aaagaaagaa gaaaagaaaa aggtcaaatc tgttcaagaa
     3721 gatgtcaaaa aagaacgtga ttttaatgat attgtttccc aatatttacc aaaataattg
     3781 agggttgatg gtggcatcta ggtggcactt tttacaaaaa gtaccatcta ggtgccatca
     3841 gtagtggtat ctgggtgcca tttaaaaaag ggttgtggtg acttctctaa aggtcttgaa
     3901 acgcaatagg cacagcctat tttcccactc tctccatgag agtggtgcgt tcaccttatg
     3961 ctctttcatt gttctaactc ttttaggcac tcataagatt caaaatgatt aaatagctgg
     4021 aggttttctc atgccaagat ttactttaat aattgaaaac gagccgttgg ttgcgtacct
     4081 cgaacagcga gctaagaaac aaacctttaa tacaggaaag aaagtcactc gaaacgaagt
     4141 gatcaaccag attcttcaaa atgaaatgat caatgaccta acaaataatc gagaagttga
     4201 tgcgattaaa gatagtttgg atgattttaa gcacatttta caacaatacg ttgatacgaa
     4261 taatgcgctt ttgtatcgtg catttgaatc tgatggcatt taaatgattg aggtgatcac
     4321 atggcacaat ctgctggctc ttatatcaac ttaatgagcg gttttgttat ttcgtttgat
     4381 tctgagtatg tacagtatat ggatcgagaa gataagaaag tgtatcaaga aaaagatctt
     4441 tctgactttg aaacggccat gacgaatgac gatcctagtc aatcgtttga aggctatttg
     4501 gattatatgg atcggaaaaa tgcgttactc gctcaatcaa aattgacagc tgaagaaaga
     4561 gtatcattag gtgagatcaa caaacgtctc gctgattatt cgactttatc caaagtacaa
     4621 atcgagatca tgcaaaagga aacccaagaa aaaatatggt cactgaatac agggatgttt
     4681 gattttaata atgatgattt gacacaaaat gaagttgaac aatacaaaga attattcgat
     4741 cgagcgaaca aaaatgaatc tgttttgttt caagatgtgg tgtctttcga tacaaaagca
     4801 ttgattgaag caggcattta caatccatat tcaaatgaat taaaaaggga accgttgatc
     4861 atggccggtc gaaagatgat gaacgttctt tatgagaaag aaaaattaaa tggatcaaca
     4921 atcagtgtcg gtgagatcca ttacaataca ggtcactttc acgtgcactt tgcgacgacg
     4981 gaagtcgaac ctacccgtga aatggttttt gttaaagagc ataatcgcta tgaacgaaaa
     5041 gggcaacgtt ccgatgaaac attagaaaaa atgaaatcga cttttgcgaa tctgatcttt
     5101 gatcgttcga gcgaattgga acgattgaat gatttgagaa atggtctgcg taatgaaata
     5161 aaaacagaaa tggcaacacc aaaaaaagaa agtgttttgt tacttgagaa attaaaagca
     5221 cagctgccgg agaatcaaag caagtggaat agtaaaaact taacgccaga aagtcgagaa
     5281 acaatgaacc agctgattga ttcattgatg aaagacaatg gccactttga agaattcaaa
     5341 cagctggcaa aagaggaaga cattttcaag aaagctactt atggtgaggg agatcctaac
     5401 aaagagaaaa aatcatttta cgatggccga atgtacgggc cggatggcat ctactatcga
     5461 ttaggaaaca gtatcctcca agaactaaag aaggaaaatg attcttccca acaagacaaa
     5521 cctttccatc gatcttcggt atcagggcaa caaaatccaa acaaagaaac gtatgcgaaa
     5581 cttaaacatg attacttcat gaggaaagat acaaatgaaa gtcgatcacc gccaaaaaga
     5641 aatccaagtg agcgaaccaa accatttaag gaatcattca agtatcgtcg gtcggtcaca
     5701 aatcaagtca aacacctgga acgggtgacg agcaatgaat ttgatcaatt caaaaataaa
     5761 gtagcctttg aaaagctgca gcgtgaaatc gaacaagcaa aaatgagtca agagtcagac
     5821 ctattcaatt aggagaaaga ggaacgtcat ggaaaattat tactttatca atagcgtgga
     5881 tccagagaac caaacgatgc gtattcaaga agtgggtgct ggcgaaatga gtgcgcaaca
     5941 ggtacgtatc acgaaagagg atgccgacgt ttattcttta atgttggatg aagcaacgca
     6001 agaaggggag ccgttgatcg ttcagttgga tctaaagtaa ggtcaaaatc ggctgtagaa
     6061 aaattttttt cgaccagtac aaataaaaac cgattttctt tttttagaaa gtcggctttt
     6121 aataaacgaa gctaatttag ttgaagtagt tgctcaataa tcgaagagct gcctggtaaa
     6181 aataaaagta gactacctag gactaacaag atccctaaaa caaaacaggc tattacgaat
     6241 ttggttgatc tagatacttc tttatttgaa acacttatga tcaacagagt tatgccgaca
     6301 gctaacgtta aaaaatacca aaatcccatt gttatcgcca actttctttg aatagttttt
     6361 acttttaaac tgtttaataa attttatcat agacaagaag aactaatcct tgtacaagaa
     6421 aaatatatgt aaagtttaaa ctttcttata ctaaataaac aaattatgta gaaaattaat
     6481 cgcaaaccac tttcaaggaa actgatagaa caatttaaaa attggtagag ttactttcaa
     6541 ttagtaaata gcagaataaa aaaagaagat cggcgaaagc cggtcttttt ttggagggaa
     6601 atttgatgga acaacaggac aataatcgac aagaaacgga caatagtcga ttagcaagag
     6661 caaaaagaaa gttgggacgt ctgaagagag agtacagtgc gcaaatttca agagtctttg
     6721 accatcaagc acaaacaaac ggtcagccga tgaatgacaa agaaaatggc cgatcttttt
     6781 ttaataggca agagcagctg gaaggtagag ctagaacttt tcttcgtgaa atagaagcac
     6841 aagaggaacg gatctcgatg ctggaacacc aaaaggaacg caaagaactc ggcctaaata
     6901 atcaaggcgg tttaatcatg agcgttgata atattccaat gatcaaagca gaaatcgaac
     6961 aagcaaaaca agggaactcg atgtattcgg ccgcaacgat tcggaaatac gaaaagcaac
     7021 tggttgagtt agaagaattg aagcgtcgat cagaaagggc ggataaacat atgaatgaac
     7081 aagcgcaaaa aatgattgaa agtggacgtc tgacacaatg gaaaaaaaat ccaatgatct
     7141 actttgtaaa aggctatcga aaactggccg ttgaattaac ggagtcggga acattcgaac
     7201 cttctcaacg gtatgcggca aaaacggaag cagaacagtc ggtcatttct gatattttaa
     7261 gcgaactaaa atttaacact gaagaaatac ccaatgctac aaaagatcaa gatgctgcct
     7321 tgaaccattt ctatcgagtg gaatttaacg aggggtcgga aagcattcgt aattttgaag
     7381 gagagatcgt ttcacaacct ttgatcgaag aaatcaaacg tttagatcga ccaatggaaa
     7441 gtgggtatta caaattttac tttcaggaaa ttaaagatgg agaaatcgtc gatgcgtttc
     7501 gtttagacgt cggggacggg gtcaatagta cccaagacta ttataaaaag cttgaatcgt
     7561 tagctgcaca agataagaca aatgccgtaa caaacgaacg tccaaaagaa gccgtgaaat
     7621 caggaaaaaa tgatgcagtt aaaaagagaa aatcagccga tgaactaaaa aaagaaacag
     7681 accaactcgt caaagacgcc atggaaaata tcaagaatca ttttgatgat ccggaagacg
     7741 tcttagaatt aggtgcgttt atgagtaagt tttacaatta ctcactaaaa aatacggcat
     7801 tgatccaaaa tcaattgccg catgcccacg ctgttgccgg tgcgaaaaaa ttcaaagaag
     7861 ctggcttttt tattcgaaag ggagaaaaag ccatacgggt tttggcgcca acaacttata
     7921 aatgttttat caacgaacag ggacgttcac agtcattatc gaaagccacc aagctacaaa
     7981 aagagaagat tctcaaagga gaattgaaaa cggaagaaag aagagcattc aaaaacgtgc
     8041 cagtctttga cattagtcag acgacggcaa cagccgatga tctgccgaaa attttcccta
     8101 atcgtcctta taactttgat ttgacgaaag ccacaaacac aaaactgtta taccaatcat
     8161 tagaaaccta tgccatatca gaaggcctaa cgatcatcga tgatctaacg actgttacac
     8221 cagtgttagg aaccgcaaaa ggggcatatg gtcagggtat cgatggctca gaaatgcttt
     8281 tcatgaatcc tcggtttact caagcagaac aagtgccaac catgatccac gaattggcac
     8341 atgcaaaact tcattcaaaa acgagtaact cggcttattc tagctcagtc aaagaatttc
     8401 aagcggagct ggctgcgtac atcgtcgccc accattacgg cattgatacg aaagaagaat
     8461 cgattcctta tattgcggct tggacaaaga atggtcatac cgttgaagat aaattggctc
     8521 aaatggaaga ggtgaaagtg ttctctcgcc aaatgattga acagatcgac acgcaactgg
     8581 tgatccaaca agaattggaa caaaaagaac aagagcaagc gcccacggta gaaaaacaaa
     8641 agctgaccga atcacaaaag acgtacaaaa cgttctcgga agagattgct tttgcgaaga
     8701 cggtatcgat cctagatatc gctcataaaa atggcattga cctaaaaaaa gattctgcca
     8761 aacaatatcg tattgcagac aatcattctg tagttattac agcccccaaa aatttgttta
     8821 ttgaaaataa tggacatttt ggcggagggc cgattgactt tgtccaaaag attgttggaa
     8881 ttaaggactt taaagaagcc gttagacata ttacagacaa tgattatgac gcaacggaat
     8941 ttgtaccaga agaaaaaatt gactacaaat atgataaaga aaatgaacgg ccttttgttc
     9001 gagcaaaaaa atatttaata gaggaacgct gcattgatga agagcttgta aatgttcttc
     9061 ataacaaagg gtttttacga caagataaac gtaataacct agtattgatt tggaataatc
     9121 aaggggaaat ctgtggaaac agtgaaaagg gaacgatcaa agactcaacc tggagaaaaa
     9181 tcgaaccagg ttccgataat tttgttggat ttaacgttag aaatggcgaa cctaaacggt
     9241 tgaaattctt tgaatctggc gttgatctta tgagctatat gacactaaat aaatctgatc
     9301 tgcaggatac ctggtttgtt tctatggagg gtttgaagcc ggaaacggtc tactattatc
     9361 tagaagagtc acagcgtgta gcgccttcag gagttgaaga aatatccttc tgtgtcgata
     9421 atgataattc tgggaaagaa tttttggaga gatttaaaaa attcacttac aatcaaaaag
     9481 aaaaacaacc aattgaattt caagcagagt ttccgcaagc tccggtcgga caagaaacgc
     9541 aaaaatggga ttggaacaat gagctggtct atcaggtaaa cgagggcgaa aatcaaagaa
     9601 aaaagtctct cgaatctgaa ccagcattga gcttatgaag taggtgaacg gatgaaacga
     9661 atgattttta caatcattca gggaataggg atggccgttg tgctatccct tcttagtatg
     9721 tggatcggcg aacaattttt ttcaacgtat cttcctagac ttcctgaagc aagacacgga
     9781 atttccattt ccgccgcaac gttctctatt gtattggcac cgatcattga agaatacttg
     9841 atacgggtca aactattgcc ttttttgatt cgacgtacga atttaccagc tgcagttctt
     9901 ttctctagtt tcattttttc tgttttacat gggcaggttt ttctactgcc ctatttttta
     9961 aatagcctgg tctattcgtg ggtttcactc aaaacaaaaa aagcctatgg atcgatgatc
    10021 gttcatagtg cttataattt tatcgccctg attcctggtt tataagaaat ctaaaccatt
    10081 tttcgttata ctgattatca atcacaataa aagaggtgtg tacgtattga ctaccgtaat
    10141 tttagcagaa aaaccaagtc aagcagcttc ctatgcaggt gcgttaaagc atagcgtgaa
    10201 gaaggatggc ttttttgaga tccaagatcc aattttttca gatgagacct ttatcacctt
    10261 tggcttcggt catttggtcg aattagcaga accaggacat tatgatgaaa aatggaagag
    10321 ttgggcattg gatgcattgc ctatctttcc gcaacaatac gattttcagg tcgcagctga
    10381 taagaaaaaa cagtttaaga tcgtttccgg actgttaaag aaagccgata cgatcattat
    10441 tgcaacagat agtgatcgag agggggaaaa tatcgcctgg tcgatcattc atgaatcagg
    10501 cgctttttct actaaaaaaa catacaagcg tttatggatc aacagtcttg aaaaagatgt
    10561 gattcgcagt gggtttcaaa acttaaaacc aggaatggac tattatcctt tttatcaaga
    10621 agcacagaca cggcaaattg ccgactggct cattggcatg aacgggagta tgctttacac
    10681 gttgaatctt cgtttaaaag gggcaaatgg aacgttttct cttggacggg tacaaacacc
    10741 aacgctttac atgatctatc aacgccaaca actgatcgag catttcaaaa aagaaccatt
    10801 ttatgaaatc gaatcattga tcaaggcaac gaatggctcg ttcaaagccc tattaagccc
    10861 gtcacaacgt ttttctacta aagaggaact acttaccttt gtttcttcta agggcgctgc
    10921 aatcggcaca caaccaggaa cgatcaaaga tcttcaaaca aaaatgaaga aaacgaatag
    10981 tcccaattta ttctcattga gcagcctgca gtcaaaaatc aatcagctgt acaaagcaac
    11041 agccagtcag acgttgaaag ccatgcaagg actgtatgaa gcaaaattgt tgagctatcc
    11101 aagaacagat acaccattta ttacagacag tgaatttcaa tacttaaaag cgaattacga
    11161 cagttatgcc cgttttttat ccatagatga acccatggca caagatcagc caagaaagcg
    11221 atatgtcgat ggaagtaagg tgcaagagca ttacgccatt attccaacga agcaagtcgc
    11281 aagtgagtca gcctttaata aattagatga tctacaaaag aaaatctatc tgctcgtcat
    11341 gaaaacaacg atcgccatgt ttttaccaga ttacacgtat gaagaaaccg taatagaaac
    11401 catggtgtca actctctgtt ttaaagcgac gggaaaagtg ccaaaagtcg agggatggaa
    11461 gatcttattc aaagccgata ccaacaagga gaacgaagag agccaaacat taccagttgt
    11521 gatgatcggt gaagcgattc aagcggaggt aaaaagcgtg cagaaagaga cgcagccgcc
    11581 gaaaccattt actgaaggca cgttgcttac agcgatgaaa acggctaata agaccgtaga
    11641 tgatgaacaa gcgatcaaga tccttcagga agtcgaagga atcgggacag aagcaacacg
    11701 tgcgagcatt attgaaacac tgaagcaaaa agaatatatc aaagtagaaa aaaataaatt
    11761 gatcgttacc gaaaaaggaa aattattgtg ccaggcagtc gaaagccagc acttattgac
    11821 gagtgctgaa atgacggcaa aatgggaaac atacttaaag aaaatcggga aaaaagaagg
    11881 gaatcaagag acctttattg ccaatatcaa gaagtttatc gtgcatttga tcgaacacgt
    11941 accagatgat gtgcagaagt tgaatttcca cacctaccaa gcggaagtta aaaaagaaac
    12001 ggataagcaa acgatcggta aatgtccgaa atgtggcgga aatgtggtgt cgaaaaagtc
    12061 tttctacggt tgttcgaatt atcccacctg tacgtttacg ttatctgatc agttccggaa
    12121 gaaaaaattg gcgaaaacca atctgaagga tttgttagtc gggaaagaaa ccgtggtttc
    12181 aggaatcaaa aaagcagaca agacgaccta taatgcaaaa atcaagttga gcgataccgg
    12241 acagatcact tttgtatcat ttgtcaatgc gccaaaaaaa gcgaagaaag tagggaataa
    12301 agcatgatca ataacgtcgt attagtagga agattaacga gagatccaga attaaaattt
    12361 acaccgaacg gggctgcagt ggccaggccg tgaatcgaaa ttttaccaat caaagcggtc
    12421 aaagagaagc tgacttcatc aattgtgtca tttggcgcaa accagccgaa acccttacca
    12481 attacgccaa gaaagggacg cttctcggcg tgactggacg tattcagaca agatcttacg
    12541 ataacccaca aggacagcga gtgtacgtca cagaaatcgt ggcagagacg tttcaattaa
    12601 tggaatcaaa agaggtgtct gagcagcgtg cgaatgagac caacgaaaca gctgaaaaaa
    12661 gcaaaccgac accgccgccg ctacctaaac aagaaaccga tccatttgca caaagcagtt
    12721 cggccatcga tttatcagat gatgatttac cattttgaga tacaaagacg aaaaaagacc
    12781 tggaaacccc aggtcttttt tgatataaaa ttcaggttca aacgacagct gtttgtatca
    12841 cctatcatga aaagaggaat aatgtgcgat tatttgatgt tcaccaattt gaattacctg
    12901 aaaaagaggt gatcgattgg gaaaaaacca aaggagagat cggatcgatg ctctctagtt
    12961 atcttgtttc aagagaaagg atgggattga cgagaatgcc gccgatccat tcaacgtatt
    13021 cgctcttaga ccaagatata ccagaaatca aacactctag ctttgttcaa gaaaatcagc
    13081 agttcaaaca agagtttctt tactttcacc acttgttttc tttgggtctg acggccatta
    13141 gccatccata taaaccagaa atcaccgatc ggagttgtgt cagcaacacg gtttctgaag
    13201 gagcggtggt ggtggctgga gcgactgaag gaacaagcat ttcactcgaa tttagacata
    13261 aaaaaacgct aaaagccttg ataaatctag gtttttagcg tggggtatgg tataatataa
    13321 gtacagtaaa gtttgtgacg gtggctatct tttgaaaggt ggtgatgctt atgaaagtaa
    13381 gtgttcactc tttagaaagg agaagccttt tgtctgtagc tgaagcatta gggctaatga
    13441 ttagtttcgg ttctttaatc gcaacgatca tctttggcat cctaacagca gtgaaaaatg
    13501 acaaaaaaaa ataaccgtca gctttagcag gtcagcggtt attttttaac ttaaatatct
    13561 gccaccgtct taaacggtgc tgtaggggag ttgttgacgc aactcctctt tacatagtaa
    13621 gtatagcaag atttcggtgt aatatcaact gtttgctcca cagtgagagg tttttagcac
    13681 gtctttttcg gataaaataa ttgaatatac aaccaagtct ccaacactta ataaagtgct
    13741 accttttact gatttttctt atctatttct ttagcaagct gtttttgatt ctcgctaaaa
    13801 tagttgtatg tttctaagtt ttctcctgtg atgtcaataa ctgcagcacc tctcatgcaa
    13861 ctaccgctca taccagtgtc aaccatgcta tgccagcctt tttgaggatc ttttcctgct
    13921 tgccgtagtt caaaattctt ttttctttga aggtgttttt tttgttcttt tttcgcatta
    13981 gatttttctt taaattttga aaacataatc aatctacctc cgtcattata atatggtaaa
    14041 attcatttca tcatatactg agttaattcc ttagtaagaa tagttgatag tgaagcttct
    14101 ttatagaata cattatacca gtttttagtt ttaaaatcat gttgatgata ttataataca
    14161 atcaaatggt aaaaaaacat atcatcaata caagtatttt atatcaattg tagaatcaaa
    14221 tcaggataac agcttttcct ttagtaagaa tgatcaatta gaggattgat gaataattga
    14281 aaggtgactt agaaggatga ctgaagaaag aaaatgctac ggtggacaat ggaaagtacc
    14341 aataacgccc tacaatcgcc gtttatactg gccacctagc tggatcgaat gtgattgtgg
    14401 tgagttagcc aaacaagttt atgttaaaaa aggtgatttt ttctatccaa atggtcatta
    14461 tttatgtaaa acgtgtcaac gggagtatcg gaaggtcgat ggaagagatc aatttatcct
    14521 gatcaaacca aatgaagaat gatgtaggtg ttaaactaat ttctattgat gatagaagag
    14581 aaaaggtttg gatagtgata gcttttaacc agagttctaa ttatttttaa tcaatgcaat
    14641 tgtttttata ataatatacg tatatccaaa tttgatcttt aaaaattcaa ataatattta
    14701 ctgaaaagag aaggtgattc aatatgctag aactaaacaa atccgaatac caaattcttt
    14761 attattttta tgaattcaat caacctttaa caaaacatga gttgctagaa agaatacctg
    14821 atttaaataa aaatactgct gcagttgtga taaaaagatt attagacaaa ggatatttgg
    14881 aagtgggaga aataaaatat tctaacactg ttttagcaag ggcgtataga ccttgtctac
    14941 cattcataga attttttaaa gatgagtacg gagaatttgc tgttgaaaag ttagtcaaat
    15001 atgcaataag ttctttagaa gataaagaaa aactaacgta tttatcaaat ctaatagctg
    15061 agaaaaaaag tttacacaca attgaatagt gtgtttatta atggagacac tggaacaaca
    15121 tccgtacaga aaatagtgag taaaatgcga acgttatctt ttttttcgaa tcgaccaatc
    15181 tgcaataatt tggttattta gcggaccgac gatccatgat ttaaagctgg aatttaaaca
    15241 taaaaataga tttgcttaca aaataaagag agctagaatt ttttctaact ctctttattt
    15301 tgatcaattt aagatttaag cctttcggaa gatatgtgct ttataagaga atgggacagt
    15361 agcttaaaat tttctatacc tatgaaatat tttcatttca taatctgatt acgagagttt
    15421 tgtatgtgtt acaaatttaa tatctgatct cataaattat gttagtaagg aagatattaa
    15481 tctatttaac ttctatagat tgttcgtaaa agtctgaatc actatctaaa tacttatcta
    15541 tattatccaa atcatttttt atttcagtaa taacgttctg atcattactc attacgaaaa
    15601 cacccaactt atttttttct tcagaggtat agggtatgaa ttcaacatat ttttgatcag
    15661 ctgtaaaagt actggtatca gataattctt cactaccaga aacagataat gagacttcaa
    15721 atttattgtt ttgagaggta aaaattaatt ctaattctct actttgggag tcatttacaa
    15781 attccgaatt aactatttcc gttgcttctt cagtttttat attagtagac acaatatgaa
    15841 ctgattgccc ttctttttgc tctactttaa aattggtgtt attgccttca gttctcacaa
    15901 tgtcaactgt atctcctgta aggttagtag cggaatcact tgtaacactt acttcgatgc
    15961 tggaactagt agttccgttg tcgttatttt cggaagtaca tccccctata accacaagtg
    16021 ctagtaaagt taaaatactc aaactagttt ttttcattat aattccctcc tcattttaca
    16081 ata
//
LOCUS       DASNNU010000058        15973 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.058, whole genome shotgun sequence.
ACCESSION   DASNNU010000058 DASNNU010000000
VERSION     DASNNU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.058"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            172..516
                     /locus_tag="U8116_001824"
     CDS             172..516
                     /locus_tag="U8116_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEN8434817.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="U8116_001825"
                     /pseudo
     CDS             517..936
                     /locus_tag="U8116_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="U8116_001826"
     CDS             complement(1028..1378)
                     /locus_tag="U8116_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434818.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="U8116_001827"
     CDS             complement(1344..2675)
                     /locus_tag="U8116_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEN8434819.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="U8116_001828"
     CDS             2931..3497
                     /locus_tag="U8116_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN8434820.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="U8116_001829"
     tRNA            3642..3729
                     /locus_tag="U8116_001829"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /gene="bsh"
                     /locus_tag="U8116_001830"
     CDS             4063..5037
                     /gene="bsh"
                     /locus_tag="U8116_001830"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN8434821.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="U8116_001831"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="U8116_001831"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEN8434822.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="U8116_001832"
     CDS             complement(7452..7898)
                     /locus_tag="U8116_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN8434823.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="U8116_001833"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="U8116_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN8434824.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="U8116_001834"
     CDS             8363..8881
                     /locus_tag="U8116_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN8434825.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="U8116_001835"
     CDS             complement(8958..9914)
                     /locus_tag="U8116_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN8434826.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="U8116_001836"
     CDS             10080..10886
                     /locus_tag="U8116_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8434827.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="U8116_001837"
     CDS             10883..11872
                     /locus_tag="U8116_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN8434828.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="U8116_001838"
     CDS             11869..12873
                     /locus_tag="U8116_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN8434829.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="U8116_001839"
     CDS             13005..13547
                     /locus_tag="U8116_001839"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEN8434830.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="U8116_001840"
     CDS             13567..14265
                     /locus_tag="U8116_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN8434831.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="U8116_001841"
     CDS             14286..14498
                     /locus_tag="U8116_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEN8434832.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="U8116_001842"
     CDS             14498..15460
                     /locus_tag="U8116_001842"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEN8434833.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="U8116_001843"
     CDS             15478..15873
                     /locus_tag="U8116_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN8434834.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
BASE COUNT         5112 a         2687 c         3302 g         4872 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaaaga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggaggggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaatcg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgtat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt ttt
//
LOCUS       DASNNU010000059        15742 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.059, whole genome shotgun sequence.
ACCESSION   DASNNU010000059 DASNNU010000000
VERSION     DASNNU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.059"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(24..722)
                     /locus_tag="U8116_001844"
     CDS             complement(24..722)
                     /locus_tag="U8116_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEN8434835.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="U8116_001845"
     CDS             complement(715..2067)
                     /locus_tag="U8116_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEN8434836.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="U8116_001846"
     CDS             complement(2290..3690)
                     /locus_tag="U8116_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEN8434837.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="U8116_001847"
     CDS             3851..4588
                     /locus_tag="U8116_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEN8434838.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="U8116_001848"
     CDS             4713..5192
                     /locus_tag="U8116_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEN8434839.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="U8116_001849"
     CDS             5189..5956
                     /locus_tag="U8116_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434840.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="U8116_001850"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="U8116_001850"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN8434841.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="U8116_001851"
     CDS             complement(6859..7461)
                     /locus_tag="U8116_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN8434842.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="U8116_001852"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="U8116_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="U8116_001853"
     CDS             8142..8726
                     /locus_tag="U8116_001853"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN8434843.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="U8116_001854"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="U8116_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEN8434844.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="U8116_001855"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="U8116_001855"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEN8434845.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="U8116_001856"
     CDS             10627..10806
                     /locus_tag="U8116_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434846.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="U8116_001857"
     CDS             10828..11853
                     /locus_tag="U8116_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEN8434847.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="U8116_001858"
     CDS             12028..13272
                     /locus_tag="U8116_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEN8434848.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="U8116_001859"
     CDS             13425..14372
                     /locus_tag="U8116_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN8434849.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="U8116_001860"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="U8116_001860"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEN8434850.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="U8116_001861"
     CDS             complement(15274..15516)
                     /locus_tag="U8116_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434851.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="U8116_001862"
     tRNA            15637..15709
                     /locus_tag="U8116_001862"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tt
//
LOCUS       DASNNU010000060        15255 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.060, whole genome shotgun sequence.
ACCESSION   DASNNU010000060 DASNNU010000000
VERSION     DASNNU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.060"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            248..1021
                     /locus_tag="U8116_001863"
     CDS             248..1021
                     /locus_tag="U8116_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN8434852.1"
                     /translation="MHGDEWQLVAKKNDGWLFFFTSIFIGAMLLIGGKQGLFALGSLI
                     INIVLLLLLLLIFTKTEGISLLNKSILFVVIGMIISILALDGWNRSSIIKISAAVTSV
                     FLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATL
                     EELTKRQPEITAKQLWLSGRQVGRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKE
                     TIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTVRRLKR"
     gene            1018..1785
                     /locus_tag="U8116_001864"
     CDS             1018..1785
                     /locus_tag="U8116_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN8434853.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="U8116_001865"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="U8116_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEN8434854.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="U8116_001866"
     CDS             3750..4070
                     /locus_tag="U8116_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEN8434855.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="U8116_001867"
     CDS             4070..4420
                     /locus_tag="U8116_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN8434856.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /locus_tag="U8116_001868"
     CDS             4587..5525
                     /locus_tag="U8116_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEN8434857.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /locus_tag="U8116_001869"
     CDS             5540..6370
                     /locus_tag="U8116_001869"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEN8434858.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="U8116_001870"
     CDS             6414..7439
                     /locus_tag="U8116_001870"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN8434859.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="U8116_001871"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="U8116_001871"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN8434860.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="U8116_001872"
     CDS             8517..8957
                     /locus_tag="U8116_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEN8434861.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="U8116_001873"
     CDS             8960..9562
                     /locus_tag="U8116_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN8434862.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="U8116_001874"
     CDS             complement(9625..10728)
                     /locus_tag="U8116_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN8434863.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="U8116_001875"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="U8116_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEN8434864.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="U8116_001876"
     CDS             12294..12689
                     /locus_tag="U8116_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434865.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="U8116_001877"
     CDS             12689..12940
                     /locus_tag="U8116_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434866.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="U8116_001878"
     CDS             12983..13378
                     /locus_tag="U8116_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434867.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="U8116_001879"
     CDS             13375..14631
                     /locus_tag="U8116_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEN8434868.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="U8116_001880"
     CDS             14643..14966
                     /locus_tag="U8116_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434869.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="U8116_001881"
     CDS             14959..15225
                     /locus_tag="U8116_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434870.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DASNNU010000061        15073 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.061, whole genome shotgun sequence.
ACCESSION   DASNNU010000061 DASNNU010000000
VERSION     DASNNU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.061"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            78..686
                     /locus_tag="U8116_001882"
                     /pseudo
     CDS             78..686
                     /locus_tag="U8116_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="U8116_001883"
     CDS             706..1734
                     /locus_tag="U8116_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN8434871.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="U8116_001884"
     CDS             1731..2489
                     /locus_tag="U8116_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN8434872.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="U8116_001885"
     CDS             complement(2575..3315)
                     /locus_tag="U8116_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN8434873.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="U8116_001886"
     CDS             3376..4629
                     /locus_tag="U8116_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN8434874.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4930..5178
                     /locus_tag="U8116_001887"
                     /pseudo
     CDS             4930..5178
                     /locus_tag="U8116_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="U8116_001888"
     CDS             5300..5626
                     /locus_tag="U8116_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN8434875.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="U8116_001889"
     CDS             5694..6407
                     /locus_tag="U8116_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN8434876.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /locus_tag="U8116_001890"
                     /pseudo
     CDS             6717..7569
                     /locus_tag="U8116_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            7690..9555
                     /locus_tag="U8116_001891"
     CDS             7690..9555
                     /locus_tag="U8116_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN8434877.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9603..11012
                     /locus_tag="U8116_001892"
     CDS             9603..11012
                     /locus_tag="U8116_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEN8434878.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            11120..11821
                     /locus_tag="U8116_001893"
     CDS             11120..11821
                     /locus_tag="U8116_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN8434879.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11980..12459
                     /locus_tag="U8116_001894"
                     /pseudo
     CDS             11980..12459
                     /locus_tag="U8116_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            13307..13882
                     /locus_tag="U8116_001895"
     CDS             13307..13882
                     /locus_tag="U8116_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEN8434880.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13882..14322
                     /locus_tag="U8116_001896"
     CDS             13882..14322
                     /locus_tag="U8116_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN8434881.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14338..15039
                     /locus_tag="U8116_001897"
     CDS             14338..15039
                     /locus_tag="U8116_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN8434882.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5084 a         2501 c         3179 g         4309 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa acacgttatt
    13141 tatttttgtt ttatctataa aatggctgaa aaatgatttt tttctaaata ctatatatag
    13201 tggcgtctat cctatatacc actaaaaaac tacaaaatct agtcttttat tttaaaaaaa
    13261 cacgtgtaga atgaagatag acttttgagt tagggagtgt ttttttatga aacttactgt
    13321 tttttgcggt tcccgttttg gtaataaaga aagttacaaa tttattgcgc aaacgttagg
    13381 aaaatacatg gcacaagaag aaatcgaact ggtttacggt ggttcatact ccgggattat
    13441 gggagtgatt agtaaaacag tactggaaaa taacggaaaa gtcacaggta tctatccgaa
    13501 tgggcttttt gaaactgaat tgcctggaaa agatgttact acatttattc ctacagaaac
    13561 gatcgatgaa agaaaggtat tattatttga aaagggagat gctgtcctcg ttttccctgg
    13621 aggtctaggt acattggagg aattctcaca acttctttct tggatagcaa tcggtctgac
    13681 gcccgacaag ccaatcggaa tattgaatat tggcggttat tacagcggct tgcaacaatt
    13741 attagaaact tttgcaaaag aggattttat ggataaaaaa tggttggatc aagtaatctt
    13801 ttccaacaat ccattggaac tggtcaatga attacgagca gtatctatta aaaatcagtt
    13861 attattgaag gaggcaaaat aatgagtcaa atatatttag caggaccatt tttttcagag
    13921 gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg aggaaaacaa aacagtcaca
    13981 agtttctact ctccacgtca tcaccaagaa agcaattatg aactattcag cgctggatgg
    14041 gcgcaggaag tatacgaaaa agatatggaa gaactgacaa atgcagaatt tgttgttgct
    14101 atccttgact ttgagcatca aacaatcgat ccaggaacgg cttacgaatt aggtgtggca
    14161 actatgttga aaaaaccgat gatcattgta caagaagaga cggtacctac gaacttgatg
    14221 attacacaaa gtctccatac gtatttaaaa tcagaccaag ctgtacgtga atatgatttt
    14281 gaaacattgc ctgttgaaac ttacgtaggc gagtacttat agaagaggaa gggttgtatg
    14341 tcagtgattt tgatttcagg aacgattggt gcaggtaaga gcagtttgac ggatatgctt
    14401 gcaaaagaga ttgattctaa accattttat gaaaatgtcg aagataacga agtattgccg
    14461 ctcttttatt caaatcctga gcagtacgct tttttattgc aaatcttctt tttgaacaag
    14521 cgatttttag caatgaaaaa tgctttagtg aacgatgaca atgtgctaga ccgatccatc
    14581 tatgaagata gcctgttgtt ccatctaaac gctgatttag gtcgggtaac ggatatcgaa
    14641 gtacagcagt acgatagctt attagatacg atgctaaacg agttagatga tgttgctcca
    14701 aaaaaacggc ctgatttgat ggtccatatc aaagtttctt tggacacgat gctagaacgt
    14761 atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg atgagacgtt atatacctat
    14821 tatgaaacgt tgaacacccg ctataatcaa tggtatgaag attttgatgt atgtcctaaa
    14881 atacaagtgg atggggataa attcgatttt gtagaaaatc cagaagatgc aaaaaaggtg
    14941 atccgtcaga tccaagaaaa attagctgaa ttggaaggaa agcctgctgg aaaatatttt
    15001 acggataacc agcatgcaaa aggtcaagaa ttaggctaaa aataaaaaac caaacgacgg
    15061 gaaagaaaaa tta
//
LOCUS       DASNNU010000062        14625 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.062, whole genome shotgun sequence.
ACCESSION   DASNNU010000062 DASNNU010000000
VERSION     DASNNU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.062"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..816
                     /locus_tag="U8116_001898"
     CDS             4..816
                     /locus_tag="U8116_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEN8434883.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="U8116_001899"
     CDS             813..1613
                     /locus_tag="U8116_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEN8434884.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="U8116_001900"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="U8116_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEN8434885.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="U8116_001901"
     CDS             3110..4819
                     /locus_tag="U8116_001901"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEN8434886.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="U8116_001902"
     CDS             5031..9389
                     /locus_tag="U8116_001902"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEN8434887.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="U8116_001903"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="U8116_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEN8434888.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="U8116_001904"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="U8116_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEN8434889.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="U8116_001905"
     CDS             11227..11520
                     /locus_tag="U8116_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEN8434890.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="U8116_001906"
     CDS             11517..11828
                     /locus_tag="U8116_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEN8434891.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="U8116_001907"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="U8116_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEN8434892.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="U8116_001908"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="U8116_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEN8434893.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4983 a         2598 c         3255 g         3789 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggctctttg tcaataagga ctgatgagtt
    14581 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctcag
//
LOCUS       DASNNU010000063        14302 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.063, whole genome shotgun sequence.
ACCESSION   DASNNU010000063 DASNNU010000000
VERSION     DASNNU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.063"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(65..1012)
                     /locus_tag="U8116_001909"
     CDS             complement(65..1012)
                     /locus_tag="U8116_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEN8434894.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1202..1471
                     /gene="rpsN"
                     /locus_tag="U8116_001910"
     CDS             1202..1471
                     /gene="rpsN"
                     /locus_tag="U8116_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN8434895.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1647..2009)
                     /locus_tag="U8116_001911"
     CDS             complement(1647..2009)
                     /locus_tag="U8116_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN8434896.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2020..3141)
                     /locus_tag="U8116_001912"
     CDS             complement(2020..3141)
                     /locus_tag="U8116_001912"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEN8434897.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3157..3507)
                     /locus_tag="U8116_001913"
     CDS             complement(3157..3507)
                     /locus_tag="U8116_001913"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEN8434898.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3637..5148)
                     /locus_tag="U8116_001914"
     CDS             complement(3637..5148)
                     /locus_tag="U8116_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN8434899.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5470..6822)
                     /locus_tag="U8116_001915"
     CDS             complement(5470..6822)
                     /locus_tag="U8116_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEN8434900.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6975..8051)
                     /locus_tag="U8116_001916"
     CDS             complement(6975..8051)
                     /locus_tag="U8116_001916"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEN8434901.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNE
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8247..9992
                     /locus_tag="U8116_001917"
     CDS             8247..9992
                     /locus_tag="U8116_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEN8434902.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10272..10709)
                     /locus_tag="U8116_001918"
     CDS             complement(10272..10709)
                     /locus_tag="U8116_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEN8434903.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(10693..12066)
                     /locus_tag="U8116_001919"
     CDS             complement(10693..12066)
                     /locus_tag="U8116_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434904.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12388..12876)
                     /locus_tag="U8116_001920"
     CDS             complement(12388..12876)
                     /locus_tag="U8116_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8434905.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12898..13500)
                     /locus_tag="U8116_001921"
     CDS             complement(12898..13500)
                     /locus_tag="U8116_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN8434906.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(13569..>14302)
                     /locus_tag="U8116_001922"
     CDS             complement(13569..>14302)
                     /locus_tag="U8116_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEN8434907.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQ
                     YQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIR
                     WYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         4083 a         3066 c         2553 g         4600 t
ORIGIN      
        1 acattttttg tctttgcccc tacaaaaatg tttcagcttt tttcgttttt tattcctgta
       61 tttcttattt ttcgaatgac aggatgattt tcccagcagg aggatgtcct tcaactagct
      121 gatgtgcttg gatgacatgc ttaagtgaag cagacagatt cattgaaata aaaacatgaa
      181 aggctccttt ttggtaggct ttgattatgc cagcaaaggc ttctgcatct agatattcct
      241 ttcttggaac aaacgattca taaaagcctt cctttttttg ccgcaaatct agatctggga
      301 ggtcatttaa tgcaacatac ctgccgcctg gtttcatgat ctggatgccg gtttctcctt
      361 tgatacttcc tttcgtcgca tcgattacaa ggtctgcctg atcagcaaat tgcagacctg
      421 gatttgtttt atcataagca gcaaatgctg aagctcctaa tttttttacc ttttcctcat
      481 ttttactgga tgcactggtt aagattcgga ttcccttttc atggagaagc tggatcagac
      541 ttgacccgac cgcacctgat gctcctagta tcattacagt atctgtcggc tggatttccg
      601 tcaaatggtt gattagatta taagccgtaa ttcctggcgt gaccattccg gctgcttctt
      661 cccaactcat tttatctgga atttttgcta cttttgctga tggcaacacg acttcttctc
      721 cgtaagtccc gccgactgca tggacagcta ctcgatcacc tacatgaaca gttgtgacat
      781 ctgaaccaat ctctgtcaca ataccagcgc catcattacc tggtacataa gggaatttca
      841 atgttcgtat ctctttcatt tctcctaaac gcaaggcaac atcataagga ttcacactga
      901 atgcttttat ttctacacga acatgtccag aaccgacttc tctgggatga gcatcgattt
      961 cttcaaaaac atcttgtgct tctccgtaac cgttaattgc aaatcttcgc atggtcacca
     1021 tctccctttt tctttaagtt tacgtttatg cctttatttt gacaaaaaaa atgtcttttc
     1081 gttttggcgc atgattcaaa acaaaatagt tgtctttttt cgcgtaacgt gttaaattat
     1141 ttatctaaaa gcgtaaagat tacgatttac gctaataaaa aataattgga ggaatttgtt
     1201 gatggctaaa aaatcaaaga ttgccaaagc aaaaaaacag caagaaacga ttgaacgcta
     1261 tgctgcttta cgccaggaat taaaagcgca aaaagattac gaaggcttgc gtaaacttcc
     1321 tttagatgct cgtccagaac gattgaaaaa tcgtgattta atcgatggac gccctcgcgg
     1381 atacatgcgt aaattcggga tgtcccgtat aaaattccgt gaattagcac ataaaggttt
     1441 gattccagga gtaaaaaaag ccagctggta atccaagtct ggctgtaatg taaaggagga
     1501 gggcgcatgg accactcctt ggatcatcaa aaaagatacg ttcgcttctt ctagcagaaa
     1561 tgctggtact agaagcagac gtatcttttt ttacgcacag ctggtccttt tttatatgta
     1621 aaggctgcat ggttacttcg ctatatttat ttagtaacag gagtcagtgc ttcgataccc
     1681 acactaattt tcaatgctcg gtcaacttct gccataatgg gtcgatccaa atggcagacc
     1741 ttttctttca atcttgattt atcgatcgtt ctgatctgct ccaacaggat aacggagtct
     1801 ctttctattc ctgttccagt agctttgatc ccaatgtgag tcggaagctt aggtttagcc
     1861 atttttgccg ttatggccgc aacaatgatc gtcgggctaa aatggttacc tagattattt
     1921 tgaataacca aaactgggcg tgttccgcct tgctccgatc ccacgactgg agaaagatca
     1981 gcaaaataaa tatctcctct ttttaccata ggatcctcct tattcttgat attctcttgg
     2041 tatccgctgt cctaatccgc aagccacttc atagtggatc gtacctaatt gatctgctat
     2101 atcctgcata gtgatttctt tgtctgcatt tttgccgata agcgtcactt ttgttccgac
     2161 aggaaattct tgaggcaatc ggatcatcag ttgatccata catacacgcc caatcgtttc
     2221 acagtagttc ccttctacca atagagaaaa accctgcatc tttcgtggcc agccatctgc
     2281 ataaccgatc ggtatcgttc cgatccactc tgcttcagga gtgatatatg tttctccata
     2341 gccgattcct tcacctgctg ggagtttttt gacttgaatc aattcagaaa ccagctcaag
     2401 tgctggctgc aatggataaa cttccggtag tgcgtgccca gagggattaa gtccgtacat
     2461 tgctactcca taacgaatca tattgccgat tgtttcatca tgccataaag ccgttgcact
     2521 attgctgaca tgtacatatc gaggaagttc gggaagtttt ttcagcactt ctataaagcg
     2581 ttctttttgc agattccaat atgtatcatc tgcctgatct gctgtagaaa aatgggtaaa
     2641 taaaccttcc cattctaata cacgactttc tttgatgaat gcagctgcct cttttacttc
     2701 ttcaggtgta caaaatccga tacgacccat cccagtgtct gcttttagat gaatcgaaag
     2761 aggcgtctct agcttacttt tctctaaaac agcagctgct gcttgcaacc attcccgtgt
     2821 acctgcagtt actgataaat catgagcaac agctacagtc agtgcgtcgt aaggaaccat
     2881 attcaagata aggatgggct gagtaaatcc agcttctctc aactcgactc cttcatctaa
     2941 atttgaaaca caaaagcccg aagctccacc tgcaactgct gcctcagctg tttcaatagc
     3001 tccatgaccg tatccgttcg cttttactac tgcaaacaat tcttttcctt gcggaagtct
     3061 tgcaacttca tttgctacat tttcagttat agctttttta tgtatcactg ctttcgttgg
     3121 gcggtgccaa gcgacaacca tcgtttttcc ttctttctag tcttccaaaa taatttgtgc
     3181 aaaagctgtt tcatttgtat gagtaatact gacccataca ttgccttcat gaggtgaacg
     3241 cgtcacaaca ggctgaccat tttcattttt taaaatctcg acatcttgaa aggtaacttt
     3301 tccgatccca gttccccaag ctttagagaa agcctctttg caagcgtaac gcccgcctaa
     3361 aaattctact tggcgatgaa aaggcaagga ctggaatagt ttcatttcat cagaggtcaa
     3421 gattcgtgca atgaattttg gtttttcttc gatcagccga gtgatcctag gaagttcgac
     3481 tgcatcgatc ccaattcctt ttatcatatg cttttctctt ttcaataaat ttagaacact
     3541 ctcattctac caaattataa ccattttgcc acaaataagt ccacaaataa caaaaatgaa
     3601 agtttctctt aaaaaagaga aactttcatt ttatgtttaa tcgttatttg aacggattac
     3661 aaatccgcgt tttttatcat tgtgacgttt accgccatct ttttggctgt tattattttt
     3721 gctataaccg ccttttgatt tgtttccacg ataattaccg ccgtcacgac gattacggtt
     3781 gtttccgcca ccacgtttgc catttttatt gtatcctttt ttctgcattg gcaatgggcg
     3841 ttctggtgtg atcttcactg gcactgcatc agctggatct tttgctgtcg tttttagcaa
     3901 aagtgcaact aaatcttgtg cagaatattc ttctaataat ttatctgctg tttgcaggta
     3961 tttatctaga ccattttctg caagttttgt ttcgatttgt tcaactgcag cacctaattg
     4021 acctttgaat gcttcttttt cggttggtgg acgtaaagtc gtcatccgtt ttttcgtcaa
     4081 gttttcaatc acatgcaagt agtccatttc atttggtgta acgaaagtca cagacatacc
     4141 gccttttccg gcacggcctg tacgtccgat acggtgaacg tagctttctg gatcttgagg
     4201 gatatcatag ttgtacacat gagtgacacc tgaaatatct aatccacgtg cagcaacgtc
     4261 tgtcgctaca aggatatcta aatgaccgct cttaaatgaa cgcaatacgc tcattcgttt
     4321 ttgttgagac aaatcgccat ggattccttc tgcacggtaa ccgcgtgctt ctaatccgcg
     4381 tgccaattcg tctacacggc gttttgtacg tccgaagacg attgttaatt caggtgtttg
     4441 aacatcaaac aagcgagtca tgatatcgaa tttttcgtat tcttttgcgc ggacatagta
     4501 ttgatcgatc aagtcagctg tcatttcttt cgctttgatc ttcacatgat gtggattttt
     4561 catgaattta acgccaatat tcttgatagc tggcggcatc gttgctgaga aaagtaatgt
     4621 ttgtctttga tcaggaactt gtgaaatgat tttttcgata tcttccaaga agcccatatt
     4681 caacatttca tctgcttcat ccaaaactaa tgtttgaacg gttccaagtt tcaatgtgtg
     4741 gcggttaatg tggtcaagca tacgtcctgg tgtacccaca acgatatgtg gacgatcttt
     4801 caagccacgg atctgacggc caatatctgc tccgccgtaa acggcttgta cacgtacttt
     4861 tttgtcgcgc cctaaacgat ataattcttc ttgtgtttgg atagctaatt cacgtgttgg
     4921 tgcgataact aaaccttgta attcgtgacg atctggatcg attttttcta acattggtaa
     4981 tccaaatgca gctgttttcc ctgtacctgt ctgtgcttgt ccaataacgt cttttccagc
     5041 taatgctagt ggaatcgttt ctgcttggat aggcgttgct tcttcaaaac cagcgcgttc
     5101 gactgatttc aacaattctg gtgataattc tagttcttta aatttcaaat gaatcctcct
     5161 atatggttgt taactgagga tgtagctttt ttgctacccc gtcgatttgt tcggtcaaag
     5221 tgtattgttt ctttttgaca gtggccacgc acgtttttat ccattcttag cccctgtggc
     5281 accaccgaaa aatcactttg cgaagtttag gcattagaaa agccaaacgg gtcgcgttca
     5341 gctttatcat tattatgaca tctggcttca acaagttgtt tctatggcaa aaccagtcca
     5401 atttctcttc atctttcggc aataggttat tctaacatag tttttcgttt tctgcaagtt
     5461 tacagatttt tatttcattt gctgaagttg ttctaccact tcaaataagc ccatgctgtt
     5521 gctgcctttt agcataatac tatctgttgc ttcaagattt tgtttgatag catcaatcaa
     5581 tttattcttt tctttttcaa aatagaacac aggtagttgg gggtattttt ggttcaattc
     5641 ttcttttagt gcatgcattt gttccccata aaggaaaatc atggcgaatt tctcaggatc
     5701 gatatgttca ctcatttgcg catgcatatt taaagactct ttccctaatt ccagcatatc
     5761 tgctaacaca gcgattctgc gtccttttgt tggcagattt ttaaaagtat ctaataccag
     5821 tcccatggct gttggatttg cattgtagac atcgctcaaa atagctgcac catttcctgc
     5881 cgttagccat tcggttctgt tttgagtgat agaaacatgg gcgagccctt ggcgtatctc
     5941 aggtatggac agtccaaaga agcgcccgat cgtatacgca atcaaagcat tcgttacatt
     6001 ataacttcca ggcagcggga tcgtaaatgt ttctccttct actacaaaat cggtttgtgc
     6061 ttttcctttt gctgtcactt ccgcatgaac atcgccattt ccaataccga atgttgtgac
     6121 tgtttgtact aatgttgccg ttaaaggtgt cagcaacggt tcatctgcgg gcacaacgag
     6181 taatccgtct gatacaagtc cgtcagtgat ttccattttg gctttagcga ttccttctcg
     6241 cgacccaaga ttttctatgt gtgcttcacc gatcattgtg attgctgcag cttttggttg
     6301 agccataagt gaaagttccg tgatttcatt ggcgtgatcc atgcccattt ccaaaacgat
     6361 cttttctgta tcttggggca tgtgcaaaat cgtgtaagga agaccaatat tattgttgta
     6421 atttccttgc gtcttatatg ttttgtattt ttcagacaag acagacgcgg ttagatcttt
     6481 cgtggtggtt ttaccattgc ttcctgtaat tgcaacgact tctgcgccta ctttttccaa
     6541 gtaataggtt gccaattgct gcatggcttt caaaggatct gctacaggaa taactggaaa
     6601 attttttgga gcttgttcaa caggcaagct ccaaaatgaa gcagatgctc cgtttgtgat
     6661 cgcttgctgg ataaattcat gtccatctcg cgtaccagca agaggaacga ataaacttcc
     6721 ttcggttatc ttccggctgt caaattcgac attggtaatc tttgtcagtg cagagcattc
     6781 gctgcccacg gcttgtgcaa tttcttgtat tgttagattc attgagatct ctcctaaaaa
     6841 aggttgtgaa aaaagcgttt ggcctaaacg gcgggttcgc atgactctca cttccaagta
     6901 aatttctcta ttcgtattta gaagctagaa tgaacgcggt tacgcaatca ctccagcctc
     6961 ttctttattt ctctttattc atttttttca aaaaaagatt gacgctgatg gtatcgattc
     7021 attcctaact ggatcagttc ttcaatcaaa tcaccgtatt tcaagcccat attttcccat
     7081 aagagtgggt acatactgaa ctccgtaaat cctggcatag agtttaattc attcaggaat
     7141 aattcatttt tatttgtcaa aaagaaatcg caccggctca atccgcttcc acctaacatc
     7201 gtgtaagcta acttcgcgta ctcttgcgct ttttgataaa cttcttctgg cacttcggct
     7261 ggaatctgca tttcgatttt attattgata tattttgctt cataatcata gaatgctacg
     7321 tcttttacga cttcaccagg caaagtcgtc cgaacgtctt catttcctaa tacagcaact
     7381 tcgatttcgc gcgcttcaat tccttgttca acgattgctc gagaatcata ctgataggct
     7441 gttgctaaag cattttgcag ctcttctcgg ttttctgctt ttgtaatgcc gacactagaa
     7501 cccatattcg ccggtttgac aaacatcgga taaagcaaag aaccttcaca ttgatcaaat
     7561 acttttttag gattttcttt ccattgattc ttaagtactg gtacataagg aacttgcggc
     7621 acaccagcag cttgtaaaat atacttggtc atgattttat ccatggcaca tgcactggtc
     7681 aatacgcctg cgccgacata aggcatattc aatgtctcta agaagccttg gatcgttcca
     7741 tcttcctcgt ttggcccatg taaaactgga aaaacgatgg ctccttcttc tttgatttcg
     7801 cccggattga tcacttttcc tgtaaagcct tcctctgtct gtccacttgg gtcccatgaa
     7861 agatgcaaga catctttgct ggcaggtttt tctgttagta atggaccttt gacccattgt
     7921 ccttcttttg taataaatac gagttgaact tggtaataat tataataaat ggcatttaaa
     7981 actgaaaatg cggaaagaat ggacacttca tgctctgcgc tgcgtccgcc atatagtaaa
     8041 gtaatcttca aaagattgtc ctcctgtatt ctaaatcgtt cagtgattta ctctcatatt
     8101 ttagcacaaa acaaaaataa acgaataact tatttataaa aaaacgagcc agcttctaaa
     8161 agaacgagcg tcatttattt tttgtcagct agattatttg tcccgttttt tataaaaagt
     8221 tatgataaag aaaaggaggg atgatgatgg cgcaaacaaa tccacgcagc cgcttggcag
     8281 aaatcattcg agtgttcatc aattacaatg tagtgccaaa ctttgtacag caaaaaaatc
     8341 ccgaacaggt aaaaaaagcc tttgaagaac taggccccac cttcatcaag ataggacaga
     8401 tgctttctgt tcgagaagat ttgctttctt ctgccttcac tcaaacattc aaaacattac
     8461 aagatagtgt acctagcgac acattttcaa cggtcaaaaa gacgatcgaa acagaacttt
     8521 ctctttcatt atcagatata ttcgatgatt tttcaaaatc gccttttgcc tctgcttcta
     8581 tggggcaggc tcatcgtgcc aagctgaaga acggtgacgc ggtagtcgtt aagatccaac
     8641 accctaacat cgccgaagag atccgacttg atcttcaact atttgaacga gcgatccctt
     8701 tgatcaaata cattcctgaa actagcgtag ttgatctaaa aggggtactc caagaagtaa
     8761 aacgttcctt gatcaatgaa atggacttct taaaagaaag tcaaaatggc gaacaattct
     8821 atcaaaagaa caatggctgg aaagagatcc gcagccccaa gatttatgac gctttttgtt
     8881 cgaaaaaagt gatcgtgatg gaagaaatgt ctggtaaaaa cttgaatcat ttaatgaacg
     8941 ccgaaaacaa aactgaaact tttatcactg gaattcaaaa caagcagctg aaacaagaag
     9001 ttgcaaagct tttagttgag aactttatga aacaggtgtt tgatgatggt tttttccatg
     9061 ctgatcctca tccagggaat ctgctattcc atgtactaac aaaagaagaa cagacacaag
     9121 ctagccgaaa aactgaaaca gtccatgaaa aagagttcgg ttcctttgct tttcgagcct
     9181 ccacttctgc agaagaccct gttgcgccat ataccatcaa ctacatcgat tttggtatga
     9241 tggggcatct ttctgcagga cttaggcaaa aactcacaca agccgtttta gcgctgtata
     9301 cgaaagatgc ttatcgaatc gaaaaagcgg tccttcgact ttgtcagcaa gaaggctcgt
     9361 ttgatgaaag tcggttccac caagaactga caagttttct tgaacagtat tatgattctc
     9421 ctattgatga aatcaacttg caagaagtat tcacacatgt cgtgacgatt tgtcatcaaa
     9481 ataatctgca gttcgatcgt gatatcacgc ttttgctgaa agcatttggg acattagaag
     9541 gggtgatccg cgtcctcgat cctgaagttt ccctcatgga agtcgcttcg ccttttgctc
     9601 aacattattt tctcactcat ctggatgtag aagatacatt gaaacaaagc ggattagact
     9661 tattggaagg gatgaaggct gcgcctaaga tccctcaaca gctccaccat ctattagaaa
     9721 tgtggacttc gggacaagga aaagtcaatt tggaattgaa aaagcaagac aaattattga
     9781 gcaggatcga aagtatgatc aatcggcttg ttttcggtat gattttagct gcattgatcg
     9841 tagggtcctc cctgcttgtc caagcagcac ctgtagaaaa cgcagaggtc gtttccttat
     9901 tagggatatt tacgtatgct attgccgcat tcgtcattat ttttttggca atcgatgcat
     9961 tgatccaaat gtataagaag aggaaaaagt agaaaaaggt tgtgacagaa agaatcagct
    10021 tcaagaaata agaaggggtt ttacataaat tgtttctcaa atttttgtga aaattcggct
    10081 tatttttcga agaggctact tctgaaacac cgagaataaa aaatgtgaaa aagctgttct
    10141 gcacccagta ataagaacaa tcaaaaaggg aacatggaac acatttatcc tgtttccctg
    10201 ttcccttttt gatgtattcg agcataaata aaataccttc gttacctact atcttctgta
    10261 cttggtaaac cttagtcaat tgcctttttt aacgcttgac gaaaagacat atgatctttc
    10321 tccgtaaagg gttttggacc ctttgtcctt atgccagctt ttcttaactt tccactatga
    10381 taacgttgtt ctagaagcgt gccgatattc gcttcgtcaa attcttttcc agagtgatgg
    10441 aacactcgca cacccttcgg caatacaagt gaagctaaac cataatcttg cgtgatcaaa
    10501 aagtcattct tcttaaccat accgacgatt ttataatccg cactatcagc tccgcggtct
    10561 acgtagacga tttggacatg attagcgtat tctttggtcg tataatggtc cacactggtc
    10621 acgataacca cgtcaagacc ataggattct cctaatttta tgacatcttc ttttactgga
    10681 gaaccatctc catcaatgaa tagcctcatt ctttttcgcc ttcttccata aatgataaac
    10741 agttactaat ccgctcagta aaagaccaac attgatccat tttgccaaga taagcagttg
    10801 tacactttct atcggcactg attcatggtt cagcgtgatc aataatagat ggattggctc
    10861 tcccatttct tttgctaaaa caaacaaaag cgtcatcaat aaaaactggt ggatcacact
    10921 ccctttattt tgagtagtat acttgacaac gatccggcga tccattggaa tagctgtatc
    10981 agtttgataa tattccttat ttagccagct gcctgttact acgtgccaga aactaagcag
    11041 aaagacacct atcgcaaaac catttggaat caagaggacc aataaagaag aactcagtcc
    11101 aagcaattgc atagccaatg gagaccatgt taaacgtttc aaaatccatg gtccgactat
    11161 ttttgctgca atcaagcctg ctgcataagg caaatacata aaaatcgaaa gtttttctaa
    11221 tccgtacagt atgccaacat aaaaactgct aaataaaaaa aggaaattcc ctagcattcc
    11281 atttagaaaa gtaaacagat ttagccagct tggcaacttc cattccttct tctggtgtcc
    11341 taaaacaaac acgaacaaga tgaacagcag caagaaacca taaatagcaa gatcaaaaag
    11401 cctttcatcg aataacaatc gggcatttct caaaagaaac aataagccaa agaatatcaa
    11461 aaataaaaca agctctcgcg tagctaccag atttcttttg atgtctttga aatccagttc
    11521 gtaacgcgga taatgactaa ccgtatggta ggccatgacg taaaaaagtg tataaatagt
    11581 aaagcttgct gcaatctgag tcggtgcagg caactcaatc gcttgataca ggagaaatag
    11641 ccagatagct gcaaataagt actttttccc atccatattg ataaaacctt gcgtcttttc
    11701 gtgaaaattg accgtggtat tagcaggtac aagccatgct gcactcagcc ctagcaaaac
    11761 acttgaaaaa taaaataaag gaaaataaaa tacgcctaat attccaagca atgaaccagc
    11821 accaccaatc agcagagcga tcatcagcaa cgtgtaacta tctagaccaa atcgtagact
    11881 gttgactaaa aatagtccag tcatccgaaa ggcataaaac agcgtaaatg gcaaaatcgt
    11941 cagccaatta tgcgagtctg ttagtcccaa aaataaaaga taaggaataa aaataaccgt
    12001 gttacttaag ataaagggcg ttgtttgtgt aaaattacgc ccaaatcgtt tgagtttttc
    12061 gaacatgaca atggctcctt aaacagtctt ataactaatc tgctcttcga ttttagcatt
    12121 aaaggggaaa taaaccaatt ttacagaata tattcttgta aaaaaaaata cagaaagaag
    12181 cataagcttc tttctgcatt tttacggtac ttgattactc aagcagacaa gcccttctcc
    12241 acaaaagtct tgcccgacac ccctgcatcc ggcaggtgat aaagatagaa atcaattatc
    12301 ctgtgcctcc aaaatcaatt aggagctctg taaaccagag tccttctcga cccgtcgcat
    12361 aaatttactt taacacagtt cctatgttta tgcgagagct tttttcattt tataatcgac
    12421 catttgaaat ccttgaccga tatctgaaat aagttcttct gtaatctcaa atccttcatg
    12481 cttataaacc tcgattgctt gatgattccc ctgattcact cgcaaaaaga tctctctttc
    12541 ctttgtttgc gcagcaagtt cttcatacca gcgaaaaact gaagaagtca atccttgacc
    12601 acgtacttta tttagcaaat ataatttact taaataaatt tttccattta tctcttcata
    12661 agcagtgtaa ccgacagtct tttcttcgct ttttaataaa aagtaggaca caccttctgt
    12721 cagttctttt tgaatctgtt tttttgactg ataagttttc agcatgtaat ccacttgttt
    12781 ttgaccaatg atcggcacaa aaacttccgg ccatacttct tgtatgactg ccatcagctc
    12841 atccagttca ttttctgtta cttttatttt ttccatacac ccacgtcctt ttttacatta
    12901 taaatgacct tgcgcttgat ggaataaaaa gagcatgatc atgctataca cacccaccga
    12961 ccacggtttc aagaaaatca caatctgcat aaattcccta aaagataatt tcgtcaaacc
    13021 agctactaga cagatcaagt cgtctggcgc aaagggaagc agcaaggtaa caactagtac
    13081 ttttttaata tctttcgttt ttcttgtcag caattcatca aatttctgat acttttttgg
    13141 cgataaaata agctggacaa aggctacgcc atagtaacgg acaagcaaaa atccgattgc
    13201 ttccccaatc actaggccaa tacaactata taaaagccca aatccattgc caaacatcag
    13261 catgcccgct actgaggaga tccctcccgg caaaatagga atgatcactt ggatcacttg
    13321 caacagaaca aatatcatca ctgcatactt gccaaattgc tggataaacg cttgcaaaga
    13381 cgtgctcgat cgaaaaatcc catgatgata agcataaaga atcaacacaa agaaaaaaaa
    13441 cagaccaata atgggcatga tctgaagaca tttttgtaaa actgatcctt ttttagacat
    13501 ctatattccc cacatctttt ttcatttatt aaaactgcct ccttatgaac tgaaccccaa
    13561 aagttgaact atttaatgga ctgtttccga tattctactg gagatagtcc atttaacttt
    13621 agttttatcc ttttgttatt ataccaccta atatactcat ttaatttact ttgaaatatc
    13681 tcaattgatc ggaatttttc tcgataaaaa aactctgatt taagcacacc aaaaaaattt
    13741 tctattacag aattatctaa gcaatttcct tttctagaca tgctttgaat aatgttattc
    13801 tcttttaatt ttttttgata ttgtggcatc tgatactgcc atccttgatc tgaatgtaga
    13861 atcagtgaac cctcagttcc ctttttttta attgcttgtt gaagcatttc ttcaatcagt
    13921 ttatatgttg gactagttga tatactataa ctaattattt ctccgttaaa taaatcaagt
    13981 ataggagata gatagatttt tcttcctttt atcttgaatt cagtgacgtc tgtcacccat
    14041 tttttattgg gtgtatctac tgaaaaattt cgttttaaca cattcttggc aatttttcct
    14101 actgttcctt tataagattt atatcgcttt attcggattt gacaggtaag tcccatttgg
    14161 gacattaatt ttctaactgt tttatgattg attgtatatc ctttcatctt taacgctaaa
    14221 gtgactctac gataaccata agagtttctt gattctttta caatcgctgt aatttcttgc
    14281 tttatcttgc tatatttatc tg
//
LOCUS       DASNNU010000064        14192 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.064, whole genome shotgun sequence.
ACCESSION   DASNNU010000064 DASNNU010000000
VERSION     DASNNU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.064"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            476..1339
                     /locus_tag="U8116_001923"
     CDS             476..1339
                     /locus_tag="U8116_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN8434908.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="U8116_001924"
     CDS             complement(1468..3090)
                     /locus_tag="U8116_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN8434909.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="U8116_001925"
     CDS             3339..3860
                     /locus_tag="U8116_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN8434910.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="U8116_001926"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="U8116_001926"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEN8434911.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="U8116_001927"
     CDS             5941..6759
                     /locus_tag="U8116_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEN8434912.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="U8116_001928"
     CDS             complement(6862..7059)
                     /locus_tag="U8116_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434913.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="U8116_001929"
     CDS             7228..7806
                     /locus_tag="U8116_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN8434914.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="U8116_001930"
     CDS             7901..8038
                     /locus_tag="U8116_001930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434915.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="U8116_001931"
     CDS             complement(8851..9354)
                     /locus_tag="U8116_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN8434916.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="U8116_001932"
     CDS             9713..10738
                     /locus_tag="U8116_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN8434917.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="U8116_001933"
     CDS             11061..11939
                     /locus_tag="U8116_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEN8434918.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12415..12531
                     /locus_tag="U8116_001934"
     CDS             12415..12531
                     /locus_tag="U8116_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN8434919.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="U8116_001935"
     CDS             13459..13797
                     /locus_tag="U8116_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434920.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="U8116_001936"
     CDS             13891..14121
                     /locus_tag="U8116_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434921.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         4503 a         2372 c         2717 g         4600 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat at
//
LOCUS       DASNNU010000065        14068 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.065, whole genome shotgun sequence.
ACCESSION   DASNNU010000065 DASNNU010000000
VERSION     DASNNU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.065"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..210)
                     /locus_tag="U8116_001937"
     CDS             complement(<1..210)
                     /locus_tag="U8116_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434922.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="U8116_001938"
     CDS             complement(216..446)
                     /locus_tag="U8116_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434923.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="U8116_001939"
     CDS             1063..1725
                     /locus_tag="U8116_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434924.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="U8116_001940"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="U8116_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN8434925.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQIAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="U8116_001941"
     CDS             5145..5459
                     /locus_tag="U8116_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN8434926.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="U8116_001942"
     CDS             5472..5846
                     /locus_tag="U8116_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN8434927.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="U8116_001943"
     CDS             5856..6200
                     /locus_tag="U8116_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN8434928.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="U8116_001944"
     CDS             6271..7623
                     /locus_tag="U8116_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN8434929.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="U8116_001945"
     CDS             7683..8825
                     /locus_tag="U8116_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEN8434930.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="U8116_001946"
     CDS             8850..9104
                     /locus_tag="U8116_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434931.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /locus_tag="U8116_001947"
     CDS             9360..10544
                     /locus_tag="U8116_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN8434932.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="U8116_001948"
     CDS             10541..10678
                     /locus_tag="U8116_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN8434933.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="U8116_001949"
     CDS             10683..10904
                     /locus_tag="U8116_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434934.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="U8116_001950"
     CDS             10917..11417
                     /locus_tag="U8116_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN8434935.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="U8116_001951"
     CDS             11514..13361
                     /locus_tag="U8116_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEN8434936.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..>14068
                     /locus_tag="U8116_001952"
     CDS             13364..>14068
                     /locus_tag="U8116_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN8434937.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHN"
BASE COUNT         4766 a         2247 c         3064 g         3991 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaaattgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataat
//
LOCUS       DASNNU010000066        13808 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.066, whole genome shotgun sequence.
ACCESSION   DASNNU010000066 DASNNU010000000
VERSION     DASNNU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.066"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="U8116_001953"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="U8116_001953"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEN8434938.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="U8116_001954"
     CDS             complement(2261..2512)
                     /locus_tag="U8116_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEN8434939.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="U8116_001955"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="U8116_001955"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEN8434940.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="U8116_001956"
     CDS             3462..3680
                     /locus_tag="U8116_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434941.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="U8116_001957"
     CDS             complement(3969..4481)
                     /locus_tag="U8116_001957"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEN8434942.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="U8116_001958"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="U8116_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEN8434943.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7377)
                     /locus_tag="U8116_001959"
     CDS             complement(6934..7377)
                     /locus_tag="U8116_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEN8434944.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="U8116_001960"
     CDS             complement(7378..8166)
                     /locus_tag="U8116_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN8434945.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /locus_tag="U8116_001961"
     CDS             complement(8163..9104)
                     /locus_tag="U8116_001961"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEN8434946.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="U8116_001962"
     CDS             complement(9281..9868)
                     /locus_tag="U8116_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEN8434947.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11396)
                     /gene="obgE"
                     /locus_tag="U8116_001963"
     CDS             complement(10089..11396)
                     /gene="obgE"
                     /locus_tag="U8116_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEN8434948.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="U8116_001964"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="U8116_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN8434949.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12785..13720)
                     /locus_tag="U8116_001965"
     CDS             complement(12785..13720)
                     /locus_tag="U8116_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN8434950.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3096 c         2435 g         4469 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac
    12781 tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat
    12841 ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct
    12901 tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat
    12961 gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga
    13021 aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg
    13081 gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc
    13141 aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg
    13201 ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta
    13261 gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat
    13321 ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat
    13381 gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa
    13441 gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt
    13501 cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat
    13561 ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag
    13621 aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag
    13681 ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct
    13741 cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt
    13801 tttataac
//
LOCUS       DASNNU010000067        13557 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.067, whole genome shotgun sequence.
ACCESSION   DASNNU010000067 DASNNU010000000
VERSION     DASNNU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.067"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            8..430
                     /locus_tag="U8116_001966"
     CDS             8..430
                     /locus_tag="U8116_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434951.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            449..2041
                     /locus_tag="U8116_001967"
     CDS             449..2041
                     /locus_tag="U8116_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434952.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2053..2388
                     /locus_tag="U8116_001968"
     CDS             2053..2388
                     /locus_tag="U8116_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434953.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            2535..2729
                     /locus_tag="U8116_001969"
     CDS             2535..2729
                     /locus_tag="U8116_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434954.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            2761..5133
                     /locus_tag="U8116_001970"
     CDS             2761..5133
                     /locus_tag="U8116_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434955.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            5194..6654
                     /locus_tag="U8116_001971"
     CDS             5194..6654
                     /locus_tag="U8116_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEN8434956.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            6665..8869
                     /gene="topB"
                     /locus_tag="U8116_001972"
     CDS             6665..8869
                     /gene="topB"
                     /locus_tag="U8116_001972"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN8434957.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            8885..9034
                     /locus_tag="U8116_001973"
     CDS             8885..9034
                     /locus_tag="U8116_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434958.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            9108..9701
                     /locus_tag="U8116_001974"
     CDS             9108..9701
                     /locus_tag="U8116_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEN8434959.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            9717..10616
                     /locus_tag="U8116_001975"
     CDS             9717..10616
                     /locus_tag="U8116_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEN8434960.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            10619..11104
                     /gene="ssb"
                     /locus_tag="U8116_001976"
     CDS             10619..11104
                     /gene="ssb"
                     /locus_tag="U8116_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN8434961.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            11470..11730
                     /locus_tag="U8116_001977"
     CDS             11470..11730
                     /locus_tag="U8116_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434962.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            12171..12377
                     /locus_tag="U8116_001978"
     CDS             12171..12377
                     /locus_tag="U8116_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEN8434963.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            12377..12628
                     /locus_tag="U8116_001979"
     CDS             12377..12628
                     /locus_tag="U8116_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434964.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            12643..13080
                     /locus_tag="U8116_001980"
     CDS             12643..13080
                     /locus_tag="U8116_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434965.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            13073..>13557
                     /locus_tag="U8116_001981"
     CDS             13073..>13557
                     /locus_tag="U8116_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434966.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CG"
BASE COUNT         5169 a         2227 c         2797 g         3364 t
ORIGIN      
        1 atcaattttg gcaaaagcaa caagaaaaca tgtatatctt tacgatatgc atatcgaagt
       61 acttgatgaa ataatttcta ctcatgaaga tatatctaat tatagtgaag cagtcagaca
      121 tttgtgtatg atgtatgatc caaaaatcga agaaaaaata aatccaattc aaattaaatt
      181 gaatgcaatg ggaaaagaaa tttctatttt aaccgaaatg gtttcagagt ttgcggatct
      241 tcatttgaaa gatacaggaa tactaattgg tacaaattca actgtttatc aagaagcaga
      301 agaacgtgtg gaaggaaaaa ttaaatccaa tcaaaccaaa aaatattctt ctaagttaaa
      361 gaaaccagtg aatgaatctg ttcataccag agtagatcca tttgatccaa cgaagattct
      421 aaaaagataa aagtagaagg agaacgtgat gaataaatcg cctgcagtta ttcttacttg
      481 tcaatttacc ttgcccaata aaaaatcttt ttcaacatac attgattata tgacaagaga
      541 aaaagcactg gaagaaatgg aacgacgtac gcccgaagaa gaacaggaac tgcaacgaat
      601 caagaatgct ttagaggatt tttatattcc tgaaggagaa actttttcgg aaagtaagac
      661 ggataaagaa ttcagtgaaa cgtcgaatga agctcaatcg ttgatggaac atggaatcaa
      721 ttttgacaag ttagaggaac aagattttac caaatatctt tcttatatga cccgtcacta
      781 tgcgttagaa caaaagaaag aactgacgca aagggaacaa aaagaatacc aacggttgaa
      841 ccaagcaatg aaaaaatatc aaacagagcc acataaaaaa gcttcaggaa atcttcctgg
      901 agttttttct atggcttcag acgaagtgaa aggaagtgat ctgaaggaga tcaaacaaat
      961 cttccaaaga ggacaacaaa aaggctcaat catttatcaa gatgttatct ctcacgacaa
     1021 ttcgtatctt gaaaaaatgg gcttgtacga tcccaaaaca gataccttag atgaagaagg
     1081 tttgaaagca gctggaagaa aaatgatgga gacattattc gagaaagaac aattgaatga
     1141 aacaggttac tggatggcaa cgattcaccg taacactaag catatccata ttcattttgc
     1201 agttgttgaa aaagaaaata cgcgtaaacc gtatactgag attgaaaatg gtgtgagtta
     1261 tatcgtaccg aaggggaaga gaagacaagc aacattagac gcaatgaaaa ccaattatgt
     1321 acatgagtta gaacgtttct cttatgaaaa ggatcgaatg atcggtatgg agaaacgaaa
     1381 tttattgaca cgaaaaagtg atttgcgtaa caccttaaca aaagtagtaa aagaaccagc
     1441 acgttacgat cgacacgcca tgaacttatt aaatgaagta tataaaaccc tacctgaaaa
     1501 acgttcggaa tggaattatg gagatgaaaa tcgaaccaag ctttcaccat ttacaagggg
     1561 aaaacttgat gagttaacca aatatgtttt aacaaaagaa cctggatatc aagaatatat
     1621 acagatttca aaagcattga aagaagaagc caaaagcctt tatggggaaa gcaaaagaga
     1681 aacaaagaat gcagaaaaaa acgccctctt tgatttaaaa aagcgcacag gaaatgcgat
     1741 tttaacagaa ctgaagagac aggatagtca aataaaacca ttaatagaaa gtgtcaatca
     1801 gatgcaatct atttacaatg aacgatcctt tcgaacactc aaacagttgc caacaaatga
     1861 atacaagtat agttatcaag aattcgtaaa gcgtaggaaa caattccaac aaagagttgt
     1921 cacgcagcga agcttgcgtc aattaaatcg aacaattaat gcacatgaag aaaaatacca
     1981 cgcaagtaaa gcatatgaag agcaacagcg aagaatccaa atggaacaag aaagacaata
     2041 ggaggaagag aaatgaaaca atatttagat caatggaaag tcatagaagg atcactaaaa
     2101 gaagaacgaa tcgaacaact tcctgattgt ttagaaaaag agcatttgtt tcagataagc
     2161 gaaatgttga gaaacgaaca gtttgatcca aatcaatttt tagttgttga gtacccagct
     2221 actggtgtgt attgttgtaa tcatgtgaag ggtgaaaaat attttattat tcaagaatac
     2281 gaaggtaagt tagcgcctta ttatactact tgggaaataa atgaagaggg aatcaataat
     2341 ttcccatgtg aaagcattga agagtctatt tctctcacgg aatgttaaat aaatagagac
     2401 gtaaaagagt cctgaacgtg attgtccagg actctttttc aatgaattgt tttccgccaa
     2461 ttcactgagt ggtgcgattc aattttatta tatcaaaaga aagtgagatt ccaaagtgaa
     2521 gggaggaaga agaaatgact cagacaacga ttggtgttgt ggttgaaaaa agcagggata
     2581 atctcgtttt tgtgacagaa attcaaacag gtagagcttt tgtagtcaca gacaaagctg
     2641 caaaagcgta tcaaaatggc aatattttaa cgttaaatct gactacgaag atgttcgttg
     2701 atgcagtaga aaattatcca tttgtttaga caaacgatat tttacaagga ggaaacggac
     2761 atggcagaag aaaaagaacc actagataat agtcggttgg gaaagtccaa aagaaaatta
     2821 gtacggttgc aaaacgaatt aaataatcaa tttcaaaagc tatttgatca ccaaaaattg
     2881 acaaatgggc aaccaatgaa tgacaaacga aatggtcgtt catggtttaa ccagcaagaa
     2941 aaaatagaaa ataaaataca aagtttgaga gaagagatca agcaacaaga aaaacaggta
     3001 gaaaaacttg aaagacaaga agagctaaag gaaatgggct ataacaagta cggcggactg
     3061 gatatgacga tcgataatat tccaagaatc aaagaagaaa tcgaacgctt tgaaaaaggt
     3121 gaatcgactt tttcagccgc cacgatccga aaatatcaac ggaagttgga aacattagaa
     3181 cagctgaaag aaaaatcaga aaaaggaaaa gaaaatatcc tccctgaagt acaagcgatc
     3241 attgacagcg gacgagtgac gcaatggaaa aaaaatccaa ccatttattt tttgaaaggc
     3301 tatcgaaaag ttgctttaga actgaatgaa aacgggggat ttgaggaatc aaaaaaatac
     3361 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctgcacca
     3421 actgattctc tgaaggagaa agaaaaagat catttctatc gagtagaatt caacgaaaat
     3481 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattaga attgatcgat
     3541 caacttaaaa cgttggataa ccaatacgct gcagaagcag gttattataa attctatttt
     3601 gaagaagtga agaacggaga agtagtagat cagatccgaa tggatctagg ggatggcgtc
     3661 aacagtaatc accccatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
     3721 gaacaaaaac aatcggtcaa aaatgaacct gtttcgaaag aacaaatgca gtcgaaagaa
     3781 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagatcaa acaagaagtt
     3841 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atacccattc ttctgaagac
     3901 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
     3961 ttgattgagg aacaatttcc aggtgcaacg gcttgtttag gtcgtgccgc gttgaaaaaa
     4021 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     4081 aaaggatttt atgagaaagg acgtggcttt gttcgagaaa cagaagctac accagaagat
     4141 aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa aaccttacta cacgattgaa
     4201 aaagtgtttg atatcacaca aacacagtta aaacctgaag actatccgaa aatctttccg
     4261 aaccgtgtct ttgattttca attggataaa aaaggacagt cagaacttca agctgggatt
     4321 caagcggtag ccaacaatat tggaatacaa attcgagata tgacagaaag tgaagtctat
     4381 caaagagaat taggacaagc acgtggggcg tatgttcgta acgaatttac tggcgaagaa
     4441 gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag tattcatgaa
     4501 ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa agaattgcag
     4561 gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacttc ggagaaagct
     4621 attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa ggaagcggcg
     4681 gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat tcaaaccatt
     4741 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc agaagtgaag
     4801 caggagcata aaaatactgt agaaacggca gagaataaga atattgtgcc cgttggttca
     4861 ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg aacgttacat
     4921 ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt aaaaggaaaa
     4981 agttatggaa aagagattca aacggcgtta ccttcagaag aatttgcgct aggaaatcca
     5041 ttccgatttt cacttgaaac agtgtcagaa gtggccaaac gaaaaaatga aatctattat
     5101 gaacaacaat tgtcacaaaa agaacgcgta taaaacaagt aaaaaagact atctgaaaaa
     5161 cggatagtct tttttctttt gggaggtttg aaaatgaccg aaaaaaaaat gagtcttatt
     5221 gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat ggctgtagtc
     5281 aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga tcgaagaaaa
     5341 caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt agctaaactt
     5401 tttttcatag acaaagagat ccaagactct aagcaacaat ttaaagcagc cactgatttt
     5461 attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga aacagaaaag
     5521 tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa ttatctgaag
     5581 gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg gttgattgcc
     5641 gaactaaagc caaaatatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
     5701 gcggtcgcct ttttatggaa agatccacag acaaaagaga cagtaggggc gagttatcaa
     5761 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
     5821 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
     5881 tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca gttaaacgat
     5941 acgtggttag tctctatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
     6001 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
     6061 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agtcgatcca
     6121 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
     6181 gaatacaaaa tcatttatga agaagtcgca aaagaagaaa aggtcacacc agaagctatt
     6241 atggccatac acaagacaga aaacaatctg caattaacga accaactggt ttccgctcac
     6301 aaagtaaatg cgttttttgg ccagcagctt tctgttaacg attcaataga agccattaat
     6361 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag tttgtgaacg cgtggatggt
     6421 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgcgca gattcttttc
     6481 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttatacct
     6541 gaagtaaaaa aggactggaa cgatcaattg aagcatgaga tccagcaaca agaaatcaga
     6601 aagcaaaaac gtgccatgtt gtttcaacaa ggcagacaac aagaaaggga gtgaaatcgt
     6661 catgatgaaa accgttattt tagcagaaaa gccgtcacaa gcgaaagctt atgccgatag
     6721 cttttctaaa gctacccgaa aagatgggta ttttgagatc caagatcgat tgtttacagg
     6781 agaaactgtg atcacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     6841 cgatgaaaaa tggaaacaat gggcattaga acatttgcct attttcccca atcaatacca
     6901 ctatcatgtt cctaaagata agaaaaagca gtttagtgtc gtcaaacgac agctgcagtc
     6961 agctgatacg attgtgatcg cgactgatag cgatcgtgaa ggggagctga ttgcctggtc
     7021 aatcattcaa caagcaggcg ccaatcaagg aaaaaccttt aaaagacttt ggatcaactc
     7081 cttagaaaaa gaagccattt atcagggatt ccaacagtta cgagacgcag aagagaccta
     7141 tccaaaattt gaagaagtac aagcacgtca gatcgccgat tggttaattg ggatgaacgg
     7201 tagcccactg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttctttagg
     7261 tagagtccaa accccaacac tctatatgat ttatcaattg caggaaaaga ttagaaactt
     7321 ccaaaaagaa ccatattttg aaggaaaagc ccaagtaatc gctcaaaatg gcgcatttga
     7381 tgcgaagctt gatccaaatg aaacccaagc gacacaagaa gcatttgaag cctatttaaa
     7441 agaaaaaggt gttcaattag gaaaacagcc aggtacgatc cttcaagtcg aaacagagaa
     7501 aaaaagtgcg gctagcccac gtcttttttc tttatcgagc cttcagtcta aaatgaatca
     7561 actcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaaggaaa
     7621 atatctaagc tatccgcgaa cggatacccc ttatattacg gaaggggaat atgcgtatct
     7681 gttggatcac ttagacgaat acaagcattt tttaaaagcc gaagcgattc cgactcctat
     7741 tcatacaccg aatagtcggt atgtgaataa taagaaagtc caggaacatt acgcgatcat
     7801 tcctacaaaa acggttatga cagcagcggc ttttgaacaa ctaagtccac tgcagcaggc
     7861 tatttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga
     7921 agaaacaacg atcttgacgc aagttcagca gcttcagtta aaggcaatcg aaaaggtgcc
     7981 gctagatcta ggttggaaga agttatttgg aaaagagagc gacggaaaag agaaggaaga
     8041 agagccgcta cttcctaaag tgacaaaagg agaaatggtg acggtggatc tacaagttct
     8101 tgaaaaagaa accaagccac cacagcctta taccgaaggt acgttgatca cggcgatgaa
     8161 aacagctggg aaaaccgttg atagtgaaga agcgcaatcc atattgaaag aagtggaagg
     8221 aatcggtaca gaagcgacac gggcgaatat tattgaaacc ttgaaacaaa aggaatatat
     8281 caaggtagag aaaaataagc tcgtggtgac gaataaaggg attttgcttt gccaagccgt
     8341 ggaaaaagag ccgctgctta cgagtgccga aatgaccgcc aaatgggaaa gctacttgtt
     8401 aaagatcggt gaacgaaaag gaacacaaac cacttttcta gccaatatcc aaaaatttgt
     8461 ctctcactta ttggaagtcg taccagggca aatccaatcc actgattttg gatcgacgtt
     8521 acaagaagtg aaagcagcca gtgaaaagca agaagccgcg cgtcatttag gaatttgtcc
     8581 aaaatgccag gaacaagaag tcttactcta tcaaaaggtg gcagcatgta cgtctgaagc
     8641 ctgtgatttc aaactatgga caacgattgc caaaaagaaa cttacagcga cacagctgaa
     8701 agagatcatt caaaatagcc gaacaagcca accagtgaaa gggttaaaag gtcagaaggg
     8761 ttcgtttgaa gcaacgattg ttttaaaaga agacttcact acaggctttg aattttctga
     8821 aaagaaaaaa acgaacttca agaaaagaac acgacgcact acaaaataat ggagggttaa
     8881 tcttatgcgt aatacaacac acaaacaaca agaaatcgaa aatcaaaaag ccaaaattcg
     8941 ttcagaacag gaaaaaatgc gtgccagcaa acgaagactg cagcaattga agaaccagca
     9001 gcggattcaa aaagaaatcg aattagatcg ataaattcct ttcttgttaa tataaaacta
     9061 acatgttata gttgtgttag ttttatatta actttagggg aagagtaatg aaagacaaaa
     9121 tccaatgtat ttttgataaa cataatggca acctatcctt gaaggatgct agaagtgaag
     9181 gattatcgcc tatgacgtta gatcgattag ccgaaaagga taaaatagaa aaggtagcac
     9241 caggtttcta taccttaaag gatgcgttcg tggatgaatt gtatctcgct cagagtatct
     9301 attccagagg gattttttct catgagactg cgctcgatct ttttcaagta ggaacatata
     9361 tccctaaaaa gatcaatatg atgtttccta aaggatacaa tgtatcaaag gagaaattag
     9421 atcagtatgc cattcaaccg cattacacta ataaagaaaa ttttgacctg ggagtcacag
     9481 aaacagaaag cttttacggc aataaaattt tagtctatga tttagagcga acgttatgtg
     9541 acatgtggaa tccaagatac aaagcaagcg tagaggtaaa acaagaagca ttaaaagaat
     9601 atatgacaag taccagtaga aatacaaata agctaagaag atacatggat attttaaaat
     9661 caccaaaaga aatgactttg tatatgttgc cactttattg agaggagtaa agatgaatga
     9721 caccgacaca atttaaagag aaaacaagaa atttcgcagc agagaaacat ttaaacccac
     9781 aaattgtcca aagatattat atgatggaaa aattattaga atgtattgtt gaatcgcctt
     9841 atcatgatga ttttgtatta aaagggggat ttctgattgg atcaaaatac gggttagaaa
     9901 accgttcaac cgttgatatt gatacgactt atcgaaattc gaaactaaca aaagaaaagt
     9961 tagaaactgt tttttctgaa ttaacagcta atcctacaaa agatggaatt cgttttgaat
    10021 taaagggatt aacagagacg agggaagccg attattatcc aggttttcaa gcaaagttta
    10081 ttgcttatct tgaaaatgca cgtgtacctt ttaaattaga tattacttca ggtgattcta
    10141 ttattccagc agcattgatg tatgaacaca aattaatgtt tgaagataaa acgattcata
    10201 ttccagctta tcctacggaa caaattctag ctgaaaaatt acatgccacg tttagttttg
    10261 gaaaagataa ttcgcgcatg aaagattttt atgacctgta tatgattccg aaattagaac
    10321 gtatcgatga gaaaaatttg tataagagtg ttcaaggaac attcaaggaa agaaataatc
    10381 aatcggtgtt taagcatttg tatgatcagg aaatgcctca attggtgaaa gatgagggct
    10441 tgagagaaaa gtggaaaaga taccaacaag aaaactattt tgccaaagac atttctttcg
    10501 aatctacact aacttccgtt gattccctga tgcaatcaat tgtagagcaa gagaaggcag
    10561 aacgaaaaca aaattttaag agatcacaac aaatgaatca agaaatggag agataaagat
    10621 gatcaataat gtaactttag tagggcgatt aactaaagat ccagatttac gttataccgc
    10681 aagtggcaca gcagtggcga ctttttccct agccgttaat cgtaatttca cgaatcaaaa
    10741 cggcaatcga gaagctgatt ttatcaattg cgtgatttgg cgcaaaccag cagaaacgat
    10801 ggcgacatta gctaaaaaag gcagcttgat cggcgttgtc ggacgaattc aaacacggac
    10861 ttatgacaac cagcaaggac aacgtgtgta tgtgacagaa gttgtggccg ataacttcca
    10921 actattagaa agtaaagcag cgacagaaag ccgtatgcac gctgatcaat cgtccagcag
    10981 tccaagccct accacctttg aacaacggga tacagcgacg cctaacaaca atggattgaa
    11041 cgcttcacag aatccatttg gtggacaatc aatcgatatt agcgatgatg atctaccgtt
    11101 ctaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt tagacaacaa
    11161 aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggc tttcttttac
    11221 gtttctatta taagaattaa cgggtaggct tactgtcttt cttggcaaat ttacaatcaa
    11281 gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca aaatcaaaaa
    11341 gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag cagacagcaa
    11401 gactattata gcataattga aatatccaaa tgattggatt tttgctataa ttttagcgag
    11461 gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat aaaaacgcaa
    11521 ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg tgtagttgca
    11581 gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa tattgccgtc
    11641 acaggtcact tcaataaacg aaatcagcta gttattgcta gcatgtatgt tagaaatccc
    11701 gatcatttta cgcgatcgat gggcatataa ataaaagaag agttgtagtc ctaaatgagg
    11761 attgcagctc tttttgtttt acatagaaac aggaaaagac ctttttagga agacacaagt
    11821 gccttctcaa ttgaaaaacc tttctctcac tccaaccgcc accgccgttc gagaagaaag
    11881 gaagtgaacg ctgcacgttc actaagatat ggtgtcttgc aaagcaagac aaaatagaca
    11941 aaacttcctc tcaccaaaag gttcgttcct gttcgttatc tctttattga tttgccagac
    12001 cggcaacaag ttgcctttta tcaccaaaaa gcgacaagga caagggtaaa tggcacgctg
    12061 cgcgcccttg tccttgtcgc ttttcgttga tttattctgg ccaacaaatc aataaagaaa
    12121 gaactaacga aaggaagtaa aacctttcct aagaggaggc agaatcaaac atgcgaaaga
    12181 aatttgatta ttggggtgtt cccttcagcg aattattccc aaactatcat gcaccacata
    12241 ctgttgaatg cgattgtgga gaaagagcga aatgtatcaa aagctatcgt ttgtatcaat
    12301 gtccaacatg cgggaaaaaa tataccttgt cttatggcga ttacattttg attgatgaga
    12361 aaggaaagaa acgctgatga aaacacaaca caaattattg actgtaaaag aagtcgttca
    12421 aattttggtg gacaaagaag tcatttctga aaaagtagca gaagctttac ttattccgcg
    12481 gtactggacg atagaaaccc ctttagtaga ggatgcaccg actatgctat ttgaagacct
    12541 agtgaagatt gcaggatatg aactgcatta tcaagaaaac atggaaactt atgaaatcga
    12601 atattggtta accaaattag agaactaaaa aggagaaaaa atatggctca aaattactat
    12661 gacgaatttg tcaaattacc tttggataaa atggcacaaa aaatggagga tatgacgttc
    12721 ctataccatg aaacacgtgt acccaaaaaa cattacaagg aaaaattgtc agtcgcagtg
    12781 gaagaaatga ttgaatcagg tgtcgaaatg aatctcattg cgacgtacta tcgaacactg
    12841 gaagaactga aaaaacaaaa tggaaaatgg tttttccaag ccttactatg cttggaagta
    12901 ggtgtcaaac caagcactat caaaccttcc gaatatcaag ccttagaact gacttatgca
    12961 aaattcgcac agattaagaa agctaaagca atggctagcg actggctaga tacttttgaa
    13021 agtatcgaaa aaaatggtgc attttatacg attgaatcgg aggagaaaga taatgaataa
    13081 ggtggtttat cagctaacaa cagaagatgt ttcgcaattt ttaacaagca aagaacactt
    13141 gaatgaagta atcagacgga ttcaaaaaga ctttagttgt atggaagaaa tgaagtactt
    13201 tattcaaaga gtggaagaac agttgtatat ggaaagcgaa acaagtcaaa cgaaggtcac
    13261 agtcagccat aatcatttag acgtgtataa acgtcgtcct gaaacggtaa aaaaaggaac
    13321 agagcttcta cgactagaaa cagaaaaata catcatgaat gtgtacctag aaaggagtga
    13381 gctggtcgaa aactttgtca gctacacgat ttgtaggaaa cactattact cagaatcaga
    13441 atattattca attttcgaga gcatggaatt aatggaagaa gaaatggaag agctaaacag
    13501 caaaaatgcg acggtagacg attattacct tgtatttact aggtttttga cgtgtgg
//
LOCUS       DASNNU010000068        13511 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.068, whole genome shotgun sequence.
ACCESSION   DASNNU010000068 DASNNU010000000
VERSION     DASNNU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.068"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(84..626)
                     /locus_tag="U8116_001982"
     CDS             complement(84..626)
                     /locus_tag="U8116_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEN8434967.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            761..1150
                     /locus_tag="U8116_001983"
     CDS             761..1150
                     /locus_tag="U8116_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434968.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1154..1918
                     /locus_tag="U8116_001984"
     CDS             1154..1918
                     /locus_tag="U8116_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN8434969.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2047..2994
                     /locus_tag="U8116_001985"
     CDS             2047..2994
                     /locus_tag="U8116_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN8434970.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3124..3864
                     /locus_tag="U8116_001986"
     CDS             3124..3864
                     /locus_tag="U8116_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434971.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4155..4823)
                     /locus_tag="U8116_001987"
     CDS             complement(4155..4823)
                     /locus_tag="U8116_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN8434972.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4957..5244
                     /locus_tag="U8116_001988"
     CDS             4957..5244
                     /locus_tag="U8116_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434973.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5660..7198
                     /locus_tag="U8116_001989"
     CDS             5660..7198
                     /locus_tag="U8116_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEN8434974.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7195..8160
                     /locus_tag="U8116_001990"
     CDS             7195..8160
                     /locus_tag="U8116_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8434975.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8147..9469
                     /locus_tag="U8116_001991"
     CDS             8147..9469
                     /locus_tag="U8116_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN8434976.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9532..9636
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9710..10453
                     /locus_tag="U8116_001992"
     CDS             9710..10453
                     /locus_tag="U8116_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8434977.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10468..11460
                     /locus_tag="U8116_001993"
     CDS             10468..11460
                     /locus_tag="U8116_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN8434978.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11472..12209
                     /locus_tag="U8116_001994"
     CDS             11472..12209
                     /locus_tag="U8116_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8434979.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12199..12921
                     /locus_tag="U8116_001995"
     CDS             12199..12921
                     /locus_tag="U8116_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN8434980.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13005..>13511)
                     /locus_tag="U8116_001996"
     CDS             complement(13005..>13511)
                     /locus_tag="U8116_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN8434981.1"
                     /translation="STISQKYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQ
                     AAAEPQAAVQEAPVQAEQPVVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKE
                     WIAQKESSGSYTATNGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKA
                     FWEANGWY"
BASE COUNT         4454 a         2306 c         2804 g         3947 t
ORIGIN      
        1 tgttttttaa tcaaaataat aaataaaaac tgtctaacat ttaaaattta atttagatat
       61 tagacagttt ttaagtttgt gagttatttt ccttgtcgtt ttctcacttt tttagtccag
      121 aaaccacctg aaatatattt tggctcataa gaaatgataa aagcacgctc ttcgatttct
      181 ttgatcaggc gatacagttc acgttcgctt tttctaggag aaagtatttc gagaactagc
      241 cgctcacctt ccagccccat tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt
      301 atacccggca aacttttatc ttctgcgcta tttgctggca aaatcgttgt gaccatgatg
      361 tatcctaatg ccaatttgtc ttcaatcaaa attccgacac tgatacctac tgcgtaacct
      421 aacgcataga caaataaatt cagtggatta tctaaacgat ctaaaacaag ggataagccg
      481 acgatataaa tcgtgatctc gaccatactg acaagtggag caatcagtcg atagcctttc
      541 attgtcaaca tgaaacgtaa cgtgttcaga gtgatataag caaaattaat gaggaagatc
      601 atccccaaca tttttggatc aatcatttgt aaaactcctt tcatttatat aacaacaata
      661 tcaaaaaaaa tcgtaagtga agaaaaattg aaactttata catacaaaaa aatgataaaa
      721 tgattataat agaattataa agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt
      781 gttttgtgcc agccacgtgc taagtctcct tctcagtata tacttaggtg tctattcgat
      841 tcaacagcca attgaggtag agaacattgt ttttcagatg tcgctgacag atggtttaat
      901 tttaagcgtt tttttctttt tgatactgtt tattggtaat attttcggta gcgttgccag
      961 ttttttgaat ttttctattt atccactcct ttcgctagca ttaggagtgg tgggactttg
     1021 ttctctgtgc ttatttcccg agccattcag tcctgcattt atattatttg gtacactgaa
     1081 cctgttccag acaactgttg gagcatggtt gctatggcga tcaggaaatt taatgaaaat
     1141 aggtgagtaa caattgaatc caattatatt gaaacgttct gtccatcata ttccagtgtt
     1201 agaagttgtt ctgaaagaat taagagagga acgtattccc gttgtcatct attatcacgg
     1261 atggcagaca agtaaggagt tagttttgac gcaaggaaga aaaattgcgc agcagggcat
     1321 tcgtgtgatc ctgccagatg cgatgaatca tggggagcgc aaacagccgg tttcttctat
     1381 tccatcctat acattttggg atagtatcta cggaaatctg tttgaatttg acacacttat
     1441 caagcattta gaaaaaagag gtcttctaca tgatcagctt gcagttggcg gggtttcgat
     1501 ggggggaatg acaacatgcg ccttgttggc taaacatcct catatagtag cgggcgtgtg
     1561 tttgatgggt tcccctgcac ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga
     1621 atataattat gtcgtaccaa aagactattt tgatttgatt tcatgggtta agagctatga
     1681 tttgtcattg cagccggaaa agttagctgg acgtcctctg ttcttttggc atggacaagc
     1741 tgacgaaaaa atcccttata tacaaacgga acgatttgta gaaaaaaatc ggcataagtc
     1801 ttacggaaaa gaaattgttt ttcgttcctc aaaaacacag cgacatatgg tacaaatacc
     1861 actaatggaa gaagcttcca cttttttagc aaaaaatttg ataaaaaagt ataaataatt
     1921 gaaacggaaa atcactcatg acgatagagg attttccgtt ttttattttg ggctataata
     1981 aactctgtta ggaaaatttg gtataattga tagtaagaat gacaacatgt tagaaaggaa
     2041 gagagcatgc ccactttagc agatgttgca aaacgtgcca atgtttcaaa aatgacggtc
     2101 tctcgagtca tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt agttttctca
     2161 gcaatggctg aactggatta ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg
     2221 tcgcagatca tcaaattatt tatactggaa gaaatcgaca caactgagcc ctattacatg
     2281 aatttgctga tgggaatcgc taaacggatc ggtaaagcgc attattctct ccaattagtt
     2341 acaaatgatg ccttcgacgt cggttcttgt gatggataca tcatcacagg tgtcagaaaa
     2401 aaagacttcg attggatcga ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct
     2461 gaaggatatg attatgtaga cagcgataat gaatatggta cagcaaaagc aacgcaatat
     2521 gccttagaaa gagattatca aacgattatt tatatcggta tcgaagtgga cgagcctttt
     2581 gaaatttcaa gggaaactgg ttatttatct gtgatgaagg aagttgatct tcctagtaaa
     2641 atcttgcggt ttccaaatca ttcgacagaa gtagaaagat atatgacaga aaactgggca
     2701 gattttacag ataaaacttg ttttgtatgt agttcagatc ggctagcact aggagcagta
     2761 agagggatta taaatgctgg aggacacttg cccgaagaat ttggtgtgat tggatttgat
     2821 ggggtatttt tagaccaaat cagttcacct aaattgacaa cgatcaagca agatattata
     2881 cgtatgggtg aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt
     2941 caaggttatc gtcgtttttt tcctgaacta gttatcagag agacaacgaa ttaagtctta
     3001 ttactatata tatatttatg aaattcatcg ctattttctt aaaataatca gcgcattgct
     3061 actgaaaaca atgaatctgt gctattattg agaaaagttc catttttgag aaggatggtt
     3121 tgaatgaaag aaatcagacg aaaacatttc cgttttcctg cgtacgatga tgagttaggc
     3181 gtaaaattaa ccaacgacaa gaagcgagta ttatttgacg gacaagaaga tcttttgact
     3241 gaacagaatc agtccccgtt tgaacagatg aaagaatttc atttcacgaa cacgaacacc
     3301 gagaaaatcc gtcgcaagcg tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa
     3361 aatttggaaa agcataaagg aaaattaccg gattataccg gccattacca atcagaaatc
     3421 acatcgacag gacgaaaaaa actttttggt gattcctctc gttctgtcag acaaagtgaa
     3481 acaaggacag agtccaagca tactacacga aacgcatctg ttgacacgaa ggtgcgggag
     3541 cgttcttatt ttgttcctaa atatgtaccc gcatcgatta tcccagaccc tagagaaagc
     3601 aaaatttctg aggatcattt aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg
     3661 ttcgatacag aaccgacgcc gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca
     3721 ttcaatcgaa caaaaacgtt agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa
     3781 agccatggta tcttagaacg ctcattacaa ggattgatcg atgatcaagg aaactctttg
     3841 ggagaaaata gctattttaa ataagtgata tacaaattta tattcggcta cagttggaaa
     3901 ggaaacgctg cggcgtttcc ttattttata ctctatccga aagtttatat cgtatcctca
     3961 aatttttttg agtttaagcg aattttttct cccaataagt aagaaaataa tggcaaaata
     4021 aaaaaacaaa ctattttcgc agatcgtttg caaaaatagt ttattttttt ataaagaaga
     4081 ttttactgaa taatatcatt ttagtttaca taatgatatt tctgtaaatt aaataaagta
     4141 gtgaaaaata agaatcagtg ctctaactgt tcaagaggat caagtggttc aaatgtttct
     4201 gtagtctgtt ttgcttccaa ttggggcaaa acagatccaa tttccagata cttcacttta
     4261 ttcaatgtat aagatagaat agaacagcta agtaatgtat acagaaaatg tgtgagatcc
     4321 aaataaagca aggagacaac taaaatacca aaagtaatca agaaaagact tttgccaatc
     4381 ttaatatgaa aatatttttc aatggcttga taaatgatat ctgttccgcc tgtgcttccg
     4441 ccgaatcgaa agacgagacc gcaaccaatg ccagaaagaa tacctgtcat caaactattg
     4501 atcagtagac tatctacagc aaaatgtata tgaaggctct cccataaatg taaaaagaaa
     4561 actagcgcaa atgttccgac gatcgtccgg agaaaagcgt gttttccaaa aagaaacaat
     4621 gagataagca ataaaggtaa attaaaaagc agtgaagtga cagaaggagc gaatccaaaa
     4681 agattatgca aaagcaaagc aataccagta accccaccat ctgccagctt agatggaata
     4741 ttgattcctg tcacagctaa tccgtagatt cctgcgccta aaatgattcc gcacaattct
     4801 gtaaataaat ttaatttttt catttctagc ctcttttctt ttgaaaattt tcagcaactt
     4861 ctttattata ctgacttata ctcttacttc aagaggaaaa tgaaaaaaaa ttatttttgg
     4921 ctgtttcttt ttatttatca aataaggagt ggaaaaatgc gagcagcaga cttattgaca
     4981 attcttacaa aatcacccaa aaaaacagag tatcaaggag tttttttggc tcaagaacaa
     5041 aaactggtgc ctgtgacaat ggtccagata aataaagcaa atcaatttat ctttttattt
     5101 gaacacggca aagcaccact tgctatgaaa gacatctttc tttttttgat gaagaaccgt
     5161 gagaaagctg tttattatca gtcaaaagaa gaactcattc ctctttatgg agtgaaagag
     5221 atagaaaata aagtggtgat ataagtaaaa ataagaaaac aagacgttta tgatgtcaaa
     5281 acaaccattt tttacatttt tggcaaaaaa gtgtcatgat acagtataat attcgttgaa
     5341 caggccaata aaggtgttct cccccacgag atttctactt tattggctgt ttctttttat
     5401 taaaataaaa gttgtgtgtg taaaaaagat atagctgatt ccaagtgaca atttcgtata
     5461 gatttttatg gtattcgtag tgaaaaaacg agaaccaaaa agttgatccg actttgttgc
     5521 ttggattctt tttttgtcag catatttaga ggtatttttt tctttttttt gttcgtgctg
     5581 taaggttaag gcaagacaag ttagttggga aaaaggaaag aaatgtttat acttaaagaa
     5641 taacagaaag gaaggtcgca tgaataattt tatacaaaca ttaagtgaaa gaaaaggcga
     5701 gttgctggaa gctgcatttc aacatctgtc catttctctc atttctttgc tgattgcagc
     5761 actgattgct attcctttag cgatttgggc agccaatcat aaaaaaatgg cagaagtctt
     5821 attgcagatc acgggtgtgc tccaaacgat tccttcactt gccttattag gtttacttat
     5881 cccatttgta gggatcggaa ctgttccagc attgattgca cttgttattt acgctttgct
     5941 tccgattttc caaaacacgt atattggtct agctgaaatt gacccgtcta ttgaagaagc
     6001 agcggtagca tttggtatgt ccagaatgag acgcttgctg aaagtagagc ttcccatcgc
     6061 attacctgtc attatctcag gtatccgaac tgccttagtt ttaatcatcg gtacggctac
     6121 tttggcagca ttgatcggag caggtggatt agggacattt attttgttag gtattgaccg
     6181 aaatacacct gttttgacat tgattggtgc gatcagttct gccttgcttg caattatatt
     6241 cagcggactg attcgctggt tgcagcataa gaaacctcga tattccatca ttactttagc
     6301 agcagtggtg tgtttatttg gcggcgtttc tttatatcag tctgccttgt ttaaaaatga
     6361 aaccatcacg attgctggaa aattaggagc ggaacctgaa atcctgatcc aaatgtataa
     6421 agaattgatt gaagacaaca caaaaacaga agttgaatta aaaccgaatt ttggcaagac
     6481 gagttttttg ttcaatgcac tggatcaagg tcaaatcgat atctatcctg agttcacagg
     6541 tactgtccta gaaagcttgg tgaaggtacc tgaagcaatg aaaagtgctg atttgacaaa
     6601 agaaaaaaca tatgacgaag cagaagaatt attgaataag caattcgata tgacattatt
     6661 aaaaccaatg gcttaccaaa atacgtatgc cttggcagtc aagagatctt ttgcgaacga
     6721 acatcaactt aaaaagattt ccgatttggc agctatctct gatcagatca aagcgggatt
     6781 cacactggaa tttattgatc gttcagatgg atataaagga atccaagaag catatggctt
     6841 gaatttttca agtgtacaaa gcatggagcc aagtttgcgt taccaagcaa tcaataatgg
     6901 agatgtcaat attgtggatg cctattccac agatagccag ctcaaggagt acgatctagt
     6961 gactctagaa gatgatcgag ggttattccc agcttatcaa ggcgcaccgc tgatgaaaaa
     7021 agattttgct aaagaacatc ctgaaatcgt taaagcactg aataaattaa gcggaaaaat
     7081 aacagaggat caaatgattg atatgaatta ccaagtcaat gtcgaaaaga aaaagccgac
     7141 agaagtagca cgaatcttcc tgaaaaatga aggattagta aaggaggaag atcagtgact
     7201 aaattaatcg agttcaaaca tgtgcaaaaa aagtatgatg gaaagtatgt catcgatgac
     7261 ctaaatctgg caatcaaaaa aggagaaatc tttgttttgg ttggtccttc cggtagtgga
     7321 aaaacaacta ctttgaaaat gatcaatgga ttatctaaac catcagcagg agacatctac
     7381 ttcaaaggaa aatcgttaaa tgaatataat ctgcagaaaa tgcggtggaa tatgggatat
     7441 gtgctgcagc aaatcgcttt gttcccgacc atgacagtca agcaaaatat tgaagtcatc
     7501 cctgaaatgt taggttggga aaagcagaaa agagcagacc gtgtagatga acttcttcaa
     7561 aaagttggct tatctcctga tatttatcga gatcgtatgc ctagagagtt gtctggagga
     7621 gaacagcaac ggatcgggat catccgagct attgctgcaa gcccagatgt gattttgatg
     7681 gacgagccat ttagcgcatt agatccgatt tcaagaaact cattacaaga attggttttg
     7741 tccttacacg aagaattggg cacgaccatc gttttcgtta cgcacaatat ggaagaagca
     7801 atcaagctag gtgatcgtat tgcatttatg aaggatggag aaattatcca atgtgacaca
     7861 ccagagcagt tattgatgaa tcctaaaaac gactacgttc ggcatttctt tgatgaaccg
     7921 aaacagacga aagagtggcg agtagaagat ttagtcgtca atggatattt cttaaatgaa
     7981 ataccagaaa atgctcgtca acaagtttgc tttgatacgc ctatgaaaga agtatattcg
     8041 cttctttcgg tgtatccaag cattgtgata gtagaaaagc aaagaagtat tgggtcgctg
     8101 acaagtaaag aaatctttgc ttttttaagt cgagaggagg aaggaaatga aaacatatga
     8161 tgcaatcatc atcggttcag gtgtgagcgg cttaagtgct gcttatggat tgaaagaagc
     8221 aggtaaaaca gtactagtgg tcgaagaaga tctatggggc gggacttgtc ctaaccgtgg
     8281 atgtgatcct aaaaaagtct tgttaagtgc agtagaggca cgtaatcggg taaaacaatt
     8341 gagtggaaaa ggttttaacg agatcccaac tgccaactgg gaagaacttc aaaaattcaa
     8401 acgaacattt acggatccag tcccagaaag ccgaaaaaaa caattagcag aagctgaaat
     8461 cgatcatctt tcaggaaccg ctcgtttttt ggatgattca tctattgaag tcaacgaaga
     8521 agtcttccat gcggattact tagttttggc aaccgggcaa agacctacga tcttaccagt
     8581 ggagggaaag gaatacttga aaactagtgc agacttttta tcattgcctg tactgcctaa
     8641 agaaatcatt tttatcggtg gtggttatat tgcttttgag ctagctacga ttgccaatgc
     8701 tgcagggagc aaagtaacca ttgtccatca taatcaacgg ccattaaaag aatttgaggc
     8761 gtctttggta gaggaagccg tccatcaaat ggaagcatct ggcattcagt ttgcttttgg
     8821 tgttgaaacc caaaagatca tttcagaagg tactcgctat cgtttagtcg gaaaagagac
     8881 ggaattagta gcagatatga ttttttgtgc gactgggaga cagccgaata cagaatcttt
     8941 agcacttgag caagcgaata tcgtctttga taaacacgga attgcagtca atgattatct
     9001 gcaaacgagc aatccaaaaa tttttgcgtg tggagatatt gtttcaagga aaaccccaaa
     9061 attgacacca gtcgccactt ttgaaggaaa ttatgtagct aagcggataa cggatgcaac
     9121 aagcgaacct atcaaatatc cgattatacc tacgatcgtc tacgccagtc ctaaacttgc
     9181 tgaagtagga gttacaaaaa gtcacgcctc ttcgtcagat caagtggtag agatggatct
     9241 taccagttgg ttcacctatc atcgtgtcaa tgaaccagtt gctaaggctg aattgacctt
     9301 tgaccagcaa aattatttga ttggtgctgc agtgattagc gagcaagcag acgaattgat
     9361 cgatgatttg acactcgtca tcaatcaaaa attgactaaa aaagagttgg atagctatat
     9421 catggggtat ccgacattgg caagtgatct ctcctattta ttgaaataat cagcatgata
     9481 actaaagcgc ttgttgcttt ttccttcttg tgctacactt taaaaaatga atacctgttt
     9541 ctggggatgt cgtagacgac atgagagtaa gttgtgaact tagacccatt gaacctgtca
     9601 gttaatactg gtgaagggag aaacatagca gacgcttgaa gcgggttact atgtcgtgcc
     9661 catttcaagc gttttttgtt attcatatta gaagaaagga ggtaacaaaa tgaagaaaaa
     9721 tctttatcca gcagtcagta tggcattcat tttaattatt tggcagattg ttgtcatagt
     9781 ttggaaaata cctgccttta ttttacccgg acctgctgct gtattttctg cattagcggc
     9841 agatgcacaa gtattacttt cccattctgt cacgacatta aatgaagcag tgcttggttt
     9901 ggtaatagct agcgttcttt cttttattac tgccttggca atggattatt ggaagttttt
     9961 agaaatgtct ttttatccgt tactagtgat ttcacaaatg ttgccaatca tggtgttagg
    10021 gccattactt accctatggt ttggtttcgg tatgcttcca aaagtgatcc ttgttgtctt
    10081 gataagctat tttccgatcg tagtttcttt ttcggataca ttgaggaaag tatcaaaaga
    10141 gcaaatcatc tttttgaaaa cgatgggggc agatacaggg aagatttatc gaatatataa
    10201 aataccagcg ggtttatccg gcttcttttc cggtttaaaa gtggctgcta cttattgtgt
    10261 cagcggagca gtcgttggag aatggctaag cgctcaagca ggcttagggt attacatgat
    10321 acgcgtaaaa aacagctacc agatcgataa agtttttgct gcgatcattt gtgtcattct
    10381 attgagttta ttgctgaatg gcgcttgttc attattgaaa aaagggtatt atcgtatgct
    10441 ttatattttt taagagaagg ggatcaaatg aaaaaattat ggcttttatt accgctactg
    10501 ttgcttttat ccgcttgtgg cactgcaaaa gaaacaagta gcaaacaaga aataaaggat
    10561 ttgaaaaaag tgacacttgt tttggattat gtaccgaata ctaatcatac tggcatctac
    10621 ctagcaaaag aaaaaggcta ttataaagag gctggtttaa atgtccaaat catcgaacca
    10681 ggagataatt cgacaagtat tggtctagta ggtgcggaca aagcacaatt tggtgtaagt
    10741 tatcaagaag atgtcactta tgctcacgca gatggacaga atataccagt caaagcaatt
    10801 gctacagtga tcaaacataa tacttctgga ttcgccaccc tatcagacag taacatccat
    10861 tcaccaaaag attttgaagg aaaaacatat gcaggttggc aaagtccaag tgaagaagct
    10921 gtcttaaaag ctgtgatgga aaaagacggc ggggactttt caaaactgac aatggtagga
    10981 agcaatggag aagggccaga aagcttagga aaatcctcag atatccaatg gtactttgaa
    11041 ggttgggata tgatcaaagc aaaagaagcg ggtattgaac taaactatat cccattgaaa
    11101 gaattagatg aacggttaga ttactatact ccggtaatta ttacgaatga tcaactgatc
    11161 aaaagtgatc cagaattggt acagtctttt atggatgcca cgaaaaaagg ctatcaggaa
    11221 gctattaagg atccaaatga cagtgctaaa ctgttgcaaa aatatgcaaa agaaaatgat
    11281 cggacgtttt tagaagaatc acaagcattc ttgtcaaaaa attatacgga tgatcctgag
    11341 aattggggat tgatggaaga aaaagtttgg cgcaattata cagcttttat gcaggaaaac
    11401 ggattgatca agcaagatgt accttcgcaa gaactattca ctaatcagtt tataaaatag
    11461 gagtgataga tatgtcaatc caaatcagcc acttgaatgt agtcattgaa aacctgaaaa
    11521 tattagaaga catccatttc actatcccta aagatacctt tacatcgatt gtcgcgccaa
    11581 gcggagccgg taagtcaaca ttgctgaaaa ctttaacagg agtccagcct atgacttctg
    11641 gaacgatcaa agtagacgat caaaaagtaa ctggtctcca tacagcattt agttatatgc
    11701 cgcaagaaga tatgttactt ccttggctga gcgtctatca aaacgttacg ttatatcaaa
    11761 aaatcaatca tctaacaatc aatgaagaac aagtaaggga ttatctggat atctttgggt
    11821 taaacggcta tgaacatttt ttgccggaac aattatctgg cgggatgaag cagcgaacgg
    11881 ccttgttacg aacaatcatg aatccttctt cttacttgct tcttgatgaa ccatttggag
    11941 cgttagatgc tatgaccaga gggcagatgc aagactggat gttgaagctg cctaaaaaag
    12001 caaagcggac tactttactt gtgacgcatg atatcgaaga agcaatttat ttatcagaca
    12061 ggattcttgt gctttccgcc cgtccggcac atgtgattgc tgagattcaa gtgcctgaaa
    12121 aacgacggtc acgtgaatgg ctactgcagc aatcagagtt gaagcaaacg atttatcagt
    12181 tattggcagg tgaattagat gttaagtgat gctcattgtc atttagatgg gagtcagagt
    12241 ttagcactcc ttcagcagga acacacgatt ttgacgatca tcaattgtga ttcgccagaa
    12301 gaatggaagg aaaacagaca gcttgcagca agtaagactc aagccttaag ttacggtatc
    12361 catccttgga aagcagatag ctatacgttt gaacaagttg agcctttttt gaaaaaagca
    12421 aggatcatcg gtgaaatagg gttagataat atttggacga atgtaccgat gactacacaa
    12481 aaaaaagtat ttgagcgaca gcttgctttt gcagccataa atgagaaacc tgttgtactg
    12541 cacacaaaag gatgcgaaaa agagatctta aattatatcc aagcttaccc taatcactat
    12601 ttgatccatt ggtattcttc tttggactat caacaagact atattgattt aggctgttat
    12661 ttcagcattg gtcttgattt gaaaaagaat ccagcagtct ggcaactagc taaagcagtc
    12721 ccaatcgacc acttacttat tgaaacggat ggactacatg cagcagagtg ggctttaggg
    12781 gggatttttt cagaaaaaga ttatgtctca ctgcttgaag aacagttaaa gacaatcgct
    12841 gagatcaaag agctatcagt tgaagaagtt aaaaaaaata cttatcaaaa cttggaacgt
    12901 ttcattagat taggtaaata aaaaagagat gtgacacaac tagtgtcgca cctctttttg
    12961 gctttctttg tataaaagta aactgtttga ttaataaatg aatcttagta ccaaccgttt
    13021 gcttcccaga atgctttcgc cgcttcccaa gaaccataac gtgaagtaac atattgttct
    13081 gctactcttt cttggtttgc agcagagtag tcaccattta agtaagaaga atctaattgg
    13141 taacgtccga tgtaacgtcc gttcgttgct gtgtaagaac cgcttgattc tttttgtgcg
    13201 atccattctt ttgcagagct tgtgcttgct ggagtagcag cagtttctgt tacagcagga
    13261 gcaggttgtg tttcagcaac aggcgctgct tgtttttccg tttgaaccgt ttcttgtaca
    13321 acaggttgtt cagcttggac tggtgcttct tgaacggctg cttgaggttc agcagctgct
    13381 tgtgcagagc cttctgttgg gattgttaat tgttgtcctg agtaaatcag gttaatatca
    13441 gaaattgaat ttgattctgc aatggcattg atcaaggagt tatcaccaac gtatttttga
    13501 gaaatcgttg a
//
LOCUS       DASNNU010000069        12954 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.069, whole genome shotgun sequence.
ACCESSION   DASNNU010000069 DASNNU010000000
VERSION     DASNNU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.069"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..1240
                     /locus_tag="U8116_001997"
                     /pseudo
     CDS             1..1240
                     /locus_tag="U8116_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1481..3097)
                     /locus_tag="U8116_001998"
     CDS             complement(1481..3097)
                     /locus_tag="U8116_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN8434982.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            3215..3433
                     /locus_tag="U8116_001999"
     CDS             3215..3433
                     /locus_tag="U8116_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8434983.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            3628..4089
                     /locus_tag="U8116_002000"
     CDS             3628..4089
                     /locus_tag="U8116_002000"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN8434984.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(4282..4521)
                     /locus_tag="U8116_002001"
     CDS             complement(4282..4521)
                     /locus_tag="U8116_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8434985.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4545..6068)
                     /locus_tag="U8116_002002"
     CDS             complement(4545..6068)
                     /locus_tag="U8116_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN8434986.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(6086..6208)
                     /locus_tag="U8116_002003"
     CDS             complement(6086..6208)
                     /locus_tag="U8116_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN8434987.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(6238..7221)
                     /locus_tag="U8116_002004"
     CDS             complement(6238..7221)
                     /locus_tag="U8116_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEN8434988.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(7245..7394)
                     /gene="rpmG"
                     /locus_tag="U8116_002005"
     CDS             complement(7245..7394)
                     /gene="rpmG"
                     /locus_tag="U8116_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN8434989.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(7415..7792)
                     /locus_tag="U8116_002006"
     CDS             complement(7415..7792)
                     /locus_tag="U8116_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8434990.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(7824..8003)
                     /gene="rpmF"
                     /locus_tag="U8116_002007"
     CDS             complement(7824..8003)
                     /gene="rpmF"
                     /locus_tag="U8116_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN8434991.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(8018..8287)
                     /gene="rpsN"
                     /locus_tag="U8116_002008"
     CDS             complement(8018..8287)
                     /gene="rpsN"
                     /locus_tag="U8116_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN8434992.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(8810..10576)
                     /locus_tag="U8116_002009"
     CDS             complement(8810..10576)
                     /locus_tag="U8116_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8434993.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(10536..12290)
                     /locus_tag="U8116_002010"
     CDS             complement(10536..12290)
                     /locus_tag="U8116_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8434994.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(12400..>12954)
                     /locus_tag="U8116_002011"
     CDS             complement(12400..>12954)
                     /locus_tag="U8116_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEN8434995.1"
                     /translation="YIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGII
                     FVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRA
                     IPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIIS
                     VLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         4087 a         2548 c         1945 g         4374 t
ORIGIN      
        1 atgttttgtt tttgatggca cattgaccta tcaaccggag gaatgctttc atcgccacta
       61 tcaaaatgaa caaacgatca ttaaataggg ctggaaaaaa gtatccatct tattgaatga
      121 tgtcagtaac tacaagacca ttctgcggat taacaaacag cgcttcaaat gtaaacactg
      181 tggaaagact tttttagcag aagattcagt tagtgatcgt cgctgctcca ttgctcgcag
      241 agtcaaacaa gccattcttg agctattaag cgaacctctt tctatgtctt tgatcgcacg
      301 tatgaagcac atttctccga ccactgtcat tcgaatactt cgcagccttc ggcctaaaac
      361 catttctttg agccagctgt taccagaagt ggtgtgtttc gatgaattca aatcagtgaa
      421 aaatgtctcg ggagccatga gctttgtcat gatggacggc aagacccatc aactgattga
      481 tattgtggaa aatcggcaac tcaatccttt acgcgactat ttcttgcgct accctataaa
      541 agtccgtgaa caagtgcgtc tagtggtttc agatttctat acaccctatc gcacattggt
      601 gaaagagtgt tttccgaatg cccgaatcgt cgccgatcgc tttcatatta gccaacacat
      661 tggtcgggct tttaccaacc accgaattca agtaatgaag acctttaaaa aaggggatcg
      721 tcggtcgaag cacttaaaga aatactggaa actacttcaa aagaatgcgt gggaattgaa
      781 aggtcagcat cgctactggc gtccgtcatt tcgagatcat ctgacagaag cagagatagt
      841 cgatcggcta ttatcttatg atgattcctt aaagcgaggc tacgaagtct accaggattt
      901 tctttccgtt atcagaagac aagatgttcc tgaattcgtc gcgctcctaa aagaagacta
      961 caaggagttg ccagagcact accaaccagt gttcacgacc ttcaagaaat accaaacaga
     1021 aatcaaacga gcgttgcgcg ttccctattc caacggccct atagaatgtt tgaataacca
     1081 tatcaaagta ctaaaacgaa ttgcctatgg ctatagaaac tttcaaaact accgagaacg
     1141 aatcttttta tatcgaggaa aatattttaa gaaaactaag aatactaccc aactgactaa
     1201 agccagaaca acaacacggc tagacaagat agctgtctag ccgtgtgagt atctcgatta
     1261 ttctattgaa tttcttcacc aacactcatt gacgaagagc caaaataagt aatctaaccg
     1321 actgttaact aaaccattgc aggtcccgtt aaagatactt gtgcctgttg tcaccgcttc
     1381 tttttcttgt ccacaagcgc ttaacacact taataaagcc cctgctatta ttaataataa
     1441 tatctttttc ttattaaaaa cctcctcatc tatttccaga ttagtaaatg tagtaatctg
     1501 tgctttcttt tttctcaata ctaataacta acttatgggg taaacaaata ctcgtttgac
     1561 cgggttttga gatccatcca gttctaaccg cgatttgatc aggactatta tcttctttaa
     1621 ctcgaattct tccatcttta atctcaacaa tattatattg tcccttcgca ggatgataat
     1681 tcacgagttt atgattgatc ttatccaaat cgaagcgatc catttccttg ccatcaattc
     1741 gaacgatggc atatttgata gcatcttcag ttgtttcatt ctccttttct atagagaata
     1801 caaataaggg aataaaagag gtaataatca aaatgccaat aattatgaca tcgcctatct
     1861 tgacaccagc ttttcttaaa aatttcatta acgatatctg gctaaacata ttagactttt
     1921 tccatggaaa tggtgaaatt tttcggaaat tatctgcatt aagagaatca gttaaacaac
     1981 cctcaagttg ttccttaata cattgttgat ccatcttcat accaataaaa actagtttat
     2041 taatgcgttc accaaacaac ggatgccagg cttccttcaa ttcgggattt ttttgtataa
     2101 tcatttgaca ctcaatttta ggtaaactag ctacccaata atttaatgat tcaatacttt
     2161 tatagctacc cgatgtttca aagatcgtcg aaaaatcact atgttgggca aaccaaagaa
     2221 acccttttgc tcttgtaata ttgccaggaa aatttttatc cgtaagatgg gcaaattttt
     2281 gtgcgtcaag gagtaaccga tttcgataga taaaacttga gataccatat tcttctgttt
     2341 caggaatatg attttgatag ccaagctcta actctttgaa ccagcccact gaataggaag
     2401 tctcatctat attaaaaaga tttgtattta gaattttcga taagtctact tcacctttaa
     2461 ttgtcctaat tattttggca ttggactgta acttagcaat tagaccgtta attttgtaaa
     2521 tcgtcttggt tgacactaag tcactattat ttaaaagtaa tatgttacaa cactcgaatt
     2581 gatcaattaa caagccacga atatcctgtt cttcttcaac tacattacct tcagcttttc
     2641 tttcctgaat cttttcaccc gtttcgaaat tatcccaaaa acgttttaca tccactacta
     2701 taataatggc accaatctct aatcgttggg tcaaatcaat gtctaaaggg tttttagcta
     2761 atataattgc ttgaataatt ggaattggtt tgctgattcc tgaagcttca ataacaattt
     2821 gattcaagtc agaaaattgc actaattgat ctagttcaac aatcaaatct tcgcgtaatg
     2881 tacaacaaat gcatccattc ggcatcgaaa caagtttttc tccagttctt ttaaaacctt
     2941 tcttgtcgac taatacccca tcaacattta cttcccctat gtcatttaca atgacagcaa
     3001 tttcctttcc gtgatcaccg tacagaatat gatttagaag tatcgttttt cctgaaccaa
     3061 gatagccagt taagattgta ataggtattt ttttcatagg gaacctcttt ctataccttg
     3121 aatttacact ttaagtgcaa cacataaaaa tgagtcataa tcgatatcca actagtataa
     3181 gtttgtcgag aacactccta gaaggaaaat aattatgagc cagtatcgtc atcttactat
     3241 actcgaacga gaaagaatct ttctgcttca cgaagaagga ttttctatta gtataattgg
     3301 caacgacatt cagcatagcc cgtttactat tttacatgaa ctgagaagaa acaagaaaaa
     3361 aactattcac cttccaaagc ggaaaaaaat tatgcttatc gaagaaaaaa atgcggacgt
     3421 tatcacattc taaatgatcc gtgagtttgg tcaattgttc gtcatttatt tgtcgatttt
     3481 cagtggtcgc cagaggaaat ctcgaatcgt ttgaagcatg aacatatgga cttttcaatt
     3541 agttttatca cgatctaacg tgcaatttgc aatgatcttt tcgatacaga acccctctca
     3601 cacggcaata aaggcctgat ccgttcattg caccatcgag gaaaaacaag acatacaaaa
     3661 aatcatgtcg agtctcgtgg aaagatttgc cttactcatt cgatccatgg ccgtccaaaa
     3721 tccgcagatg atagaagtga ttttggtcat tggaaagccg atattgtagc tggcaaaact
     3781 ggtggtactt gtttagtgac tttgacagac agaaaatcat gcttcctttt agcaggaaag
     3841 aaggtcccca gaaagaaagc cgcttatgtg agcgagaaaa tgattgaact tttaagcagt
     3901 attccaaatg aggcggtcaa aacgatcact ccagatagag gaaaagaatt ctccaatcat
     3961 ttcgcagtta cagaagcatt gaataatatg cccttttatt ttccagatcc tcatgccctt
     4021 ggcaacaatg aacaaattaa aatacgaata gactcattcg agaatacttg ccaaaatcga
     4081 aagagatagc tacggtagat aactcaacga taaatcagat gattgaaaaa ctgaataaca
     4141 gacctagaaa gtgtttagat tggagaacac cttatgaagt cttctttgat aaagtgttgc
     4201 gcttgatttg acaattcaag acataataaa aagcagatca aatccaaata agggaaaaat
     4261 gatctactct ggtattatca attaattggc caagctatta tgccgttttg ctaaacgtct
     4321 acttcttcca gtattgacct tgataaaaca acaatcagga tcccgtttta atactctatg
     4381 aactgtgtca atactaacat gtaaagttgc agcaatatca gctaatgtac tcccatttaa
     4441 tctcatttgc tttgcaaatc gataacgccg ataagcatca atattcttgg aatccataga
     4501 caataatact tttcttacca tgtatttcct ccaaaaattg aattttaatg agccatcatt
     4561 tcttgagcaa tttcatgttt agacaatgaa gctggataga aagtaggcca attatgcaat
     4621 tcattataga gtttttcttg actttctcca ccaaagaaaa tatggaaatg ctctgcctta
     4681 cttggtgcaa ttccatgatc actaaattga atatatttaa aacgaccagc acctgcatca
     4741 tcagtttcaa aattaaaacg aacaccacgg tttccttttt cataattcaa aattttgtat
     4801 ccaacatatt tgtatgtgta ttgatgatgt tcaccgttga ctacaaaatc aatggtgttg
     4861 tctttaatgt tgatagtatc aatatctgtc ttatagccag tagtataata atctttgtac
     4921 tcggcagctg tcatgtcttt attgattttt gctttgtaat cgaaaacttc atccaaaatt
     4981 ccatctttta acaacggata aacagattgc cattcgcctg tataatcact caattcgcga
     5041 tccttcacat cggcatcttt aaagtaccca ttagcaactg tcttttcatc cttcgcttct
     5101 ttttctggaa ctacctcttc accagcaacc atagttgttt tttctaacgc ttttagattg
     5161 tcttccatta ctgaaacata atcttcacca ttatccattt gttcttttgt aagactttca
     5221 agaggattca gtacttccaa ttttatccca gcttcgttgg ccaaagtttt tgcaatttta
     5281 tcattagcat tcttttcaaa gtaaatataa ttgatcttat tttttttaac atactctttt
     5341 aattcagcta atcgaccaga agatggctct tcttcaggac ttagtccagc aataggaact
     5401 tgtttcaatc cataatccaa agctaaatat ccaaaggctg cgtgttgcgt cacaaaactt
     5461 ttttgtttcg cttctttaag actagtggta tagtcagcat ccaaccgttt cagtttagtc
     5521 aaatactttt cagcatttgt ctcaaatact tttgcttttt tcggatacaa tttaactaac
     5581 tgatccttga tatttgaaac ttccttaatt gccattttgg gtgaaaccca cgtatgagga
     5641 tctagttcat gatggtgatc ttcttctccg tgatcatgat cttcttcttc actacctggc
     5701 aataaaatca tgtcttttgt tccttctact acattaggcc cagctttctt ccaactttct
     5761 ttagcttttg gtacccatga ttccatgttt tcgttatgat atacaaaaac atcagcatca
     5821 tgagcttttg ccatatcctt agctgagggc tcatagtcat gaggttctga tcccgctgga
     5881 accattaaat ccacgttcgc ttcatcgccg acgatgtttt tggtgaaatc atacattgga
     5941 tagaatgttg ttacaatttc taattttcca tctttatcat tcgatacttt tgcatcatta
     6001 gtgctggagc aagctcccaa taatcctaga gctgctatcc caataagtcc tacactgatt
     6061 ttcttcattc tttttccttc cttaattatg ctaatttctt cattttattt cgtttatgtt
     6121 ctttgataat ttttaatgcc ctttttctag ttttaatatt cgggctttta agtcgtcgat
     6181 atgcactaga tatatccgtt ttctccatta gaaattaccc ccaatcacta cttatcatta
     6241 tttgctttcc gccagtaatt tatcaaaact ctgtttgatc ctttcttcat ccaacatttt
     6301 tccgattaga ataatagtgg tttggtcttt ttgtcgagtg gtgtattgaa aattaacctg
     6361 ttcgtttacc tcttgcatgg caactataaa atcctgctct tgtaaagcaa tcaatccttt
     6421 aaaacgatac aggctccctt ggttggttaa aatcagccag tcaatccatt ggagtaaaaa
     6481 atcttttttt atgtcttgat ctgaagttaa gagaattgat ttaaactcat gtacttgatg
     6541 gtgttgatga tagtatttgc cattatagtg attttctgat gaatcacttt caaaaactaa
     6601 gggatgatta aattttttca aattataaaa atcctttttt tgaatcgttt cactatgtgt
     6661 gaacattcga atatcggata aagggttaat acttttgatt tctctctgta atttttttag
     6721 tgcagataaa ttcaagtttt gttttctaga cacaaaaatg cgatctgcca tgacaagttg
     6781 cttaagggct tgtggttctc tttcaataag ttcaaaactg tcactatcaa taacagtcaa
     6841 taaactatcg atatatactc ttcctcttag ttgaggcgct gttaaaagtg tttgaatgat
     6901 tggttgggga tctgctatac ctgtagtttc aatgatgatt tgtgaaattg gttgttgatg
     6961 ttcttctatt gtttccagta aagaaatcaa tattgctaat aaatcactgc gaagactgca
     7021 acacatacat ccattattta gttgtaacac ccgttcctcg ctatgaacta ataatttgtg
     7081 atcaatccca acttcaccaa actcattttc aattataata atttcttccc tggggtaggg
     7141 tgagacttgc aaggcttgat tgattagcgt tgtttttcca gcccctaaaa acccagaaac
     7201 gattgtaact ggtatgccca ttttgtcacc tttttaccac ttagctattt cacttcttta
     7261 aaaattgccc gtctgcgcaa ttttggcgaa tactttttta attctaaccg ttcagggtta
     7321 ttccgtttat ttttgctggt gagatacaga cgttctccag tttcgacaca ttccaaaata
     7381 atattttgac gcataaatct tcctccttca atacttatcg atcttcaaga tatgcttgaa
     7441 tttgttgtgc atcttcaaaa gttaactcgc cagattccaa ttgtgaaact aaattttctt
     7501 tcatatcaac aggtatctcc caataaccat cgatacattg aaataactcc tcgtcatact
     7561 cttcaaaaat tggatagatt cttaaaaaat catcaaatga taagccttgg tcctcatagt
     7621 aatctttgac taatccattt tcaattgcct gttgggtcgg taaacccgtt tcagtaataa
     7681 ttcctctctc taccatcgaa cgataaaaag tgcatttatc tagcgattgc tgggtcgttt
     7741 caagataata ctttaatcct tcttcaacta aaggtgggta aataatttcc attttgattc
     7801 cctcctaaaa gttatatatt aaactatttt gaagctcctt taattacttt ctttccatta
     7861 tagtaccctt ttaaagatac atgatggcta cgtctataat caccattcgc ttcgtcaaat
     7921 gaaatttctg gttttactac agtttgatga gttcttctta accgtttctt cgccttagat
     7981 gtttttcttg ctggtactgc catgatattt cctcctttta ccaacttgct ttttttactc
     8041 caggaatcaa cccttgatgg gcaagttctc taaacttaat acgggacata ccgaactttc
     8101 gcatataccc tcttggacga ccatccgtct gatcacgaag cctcagacga ttcggatttg
     8161 aatctcttgg caatttagcg agagcggtgc gatctcctgc ggcttttaat tcctgacgtt
     8221 tatctgcata cttttcaatc atcgccattt gtttctttgc tttagcaatt ttcgactttt
     8281 ttgccatata cctttatact ccttttattt tgatttagac acctcaaatc gtaatcgtta
     8341 cgttttgaca ctgatacttt accaacaaga gttgctcttg tcaacaataa ataacatgaa
     8401 ataaaactca tatgaataga taactatttg aaaacgaatt tatcggtaca tagagataat
     8461 caaatcattt aaaagaatat gcagtttagg ctctgaaaaa taagaattca cttttattag
     8521 tccaatcaaa cacaaatacc gttttaagta gcttttcctc tacttaaaac ggcaatatga
     8581 aagaaactat aactttttat tcaatctttt atgtttaact ggtttatcgc caatttttta
     8641 aaactttgat tattccttat attataagaa tagacattaa gtgaactaat tcaatcatac
     8701 tgattctttc gtgtattcaa aattagatgc tgtttaataa aattattctt tttgaaaaag
     8761 aattataact atatcccaaa aattgtaggt taatttaaag cctatttcat catcgtgatt
     8821 gagcttcttt ttcatagacg aatttctcag atagtttagt tttctttatg agatttttgt
     8881 atatttctga ccttcgatac aactcattat gagtaccttg atcaactagt tttccttgat
     8941 ccatcactac aattttattt accttttcaa tagacttcaa gcgatgcgat ataattagta
     9001 ctgtcttatc ttgtattaaa cgatttaaac tttcctgaat acgtttttca ttttccgcat
     9061 ctaaactggc agtaatttca tctaaaatta aaattggcgc atttttcaaa aaagcccgtg
     9121 ctatggatag gcgttgtcgc tctcctccag aaagtgaact tccgttttct ccgaccaatg
     9181 tatgataacc gtcaggtagt ttttcaataa agtcttcgca gttagctaaa cgagctgctt
     9241 ttttcacttc ctcatctgac gcttgactat ttccaatacg tatattttcc aaaatacttg
     9301 tattaaacaa agttacctct tgaaaaacaa tagcgatttt gctaaacaaa gaagctggag
     9361 atacacgttt aatatcatac ccatcaatta aaatacagcc ttcatcataa tcatacagtc
     9421 tagacactaa tttcaagata ctagttttcc cggaaccact agcacctact aacgcagtca
     9481 cttctccttg tttagccgta aaagagatat gatctaaaat tggggtattg ttatcatatg
     9541 agaaagagac atctcgcaac tcaatgtcaa aagatttcag aggactatct gatccctctt
     9601 ggatactggt ttccttcata gctctaattc gctgtatttt aggagctaaa taaaatattt
     9661 caagcactgc ttctttcata gaatcgaagg aatcctttat ttttattgcg gctagtaagt
     9721 aaccaacgac gtagagtata gtcacttcac ccgttaacag taagtgaaca cctactagaa
     9781 tgactactgc taaactaaca tatgagaaaa tagaggataa ggccatgact gaaaaagtac
     9841 ttaattctac ctttaaatga atcctttcac tctcttccat ttttttgaaa agtctgtctt
     9901 gaactttttt agatagatta aagctattaa tctcttgctg caattctata gtttcttgaa
     9961 attcttccga gttttctctc aaagtatcgt aatatttagt attggctttc gtttgggatt
    10021 tctttgataa caagattaag acaaaactaa ataacgttgg caaaataaca gctaatccca
    10081 ttttgacatt accaaccaaa agcatcactg aaataaaagg gaaaaacaga gccataccgc
    10141 cggctttagg tattgcatga ctcatcgcat gctcaatacc ttcaacgtca gacatgatag
    10201 tttgagataa atctgataaa ttatgttttg aaaaatagga taatggtaaa tttgacaaat
    10261 cgtctgcaat ttgcactctt aaatgcgcac tttctttata agtactatta tataagtttt
    10321 catactccct atctaacaac caatacatga aaagcaaagt aacaaacgat acggcaaaat
    10381 aaagccaacg gggctttgta ttttctaaaa ctaattcgtc tatcagcatc attataatga
    10441 atgctggtac catattaatg cagtatacaa aaaaactaga aatagaagct ttagttaaag
    10501 cttctgcgcc ctgatcactt aaagcaaatt tgtttttata aaaacttttc attgtcaagc
    10561 ctccaatcat ttgcattctc ataattagtg accaattgtt tatacacttg atgtttagcc
    10621 attaattcat tatgatttcc acgttctact attttccctt ctgacaagac aagaatttca
    10681 tctacagatt taatgctgga taatcgatga gctatcataa taactgtttt ttctttcatc
    10741 agatgttgaa aagccttttg tagttcatac tcattatctg gatcaataga tgcactggct
    10801 tcatccataa taacaatctt tgcatctttt aaaattgctc tggcaatagc tatacgttgt
    10861 ttttctcctc ctgacagata aactccttta ctaccaatta aggtatgctc tctttcggga
    10921 aatttttcta aaatcgattc gcaaccggct aatcttagcg cgtcaaatac tttacttgct
    10981 ggcgcttgag gatttgctat agaaacgtta tcgtaaatac tctgattgaa tagtttcgta
    11041 tcttgaaaga caaaagcaat tgtgttaata attgtgtctt ttgaatagtg atttaaaggc
    11101 ttaccaccaa ttttaatctg tcccttatcc acattgtaga aacctgaaat tagtttagct
    11161 atcgtactct ttccgcttcc tgattttcct actaatgcat aaattctttt ttgttctaaa
    11221 actaagttga aattttctaa aacacactct tctttgtatg aaaagctgac attttcaaat
    11281 gcaatgtcac aatttttaat ctgtgtctcg ttcccatatt ttagttggtc ttgcttcatt
    11341 tctaaataaa gggagtctag tgtttcgact gcatatcttg cttcaaacaa gtgcatactt
    11401 acatacatta ttctcatgaa agaaacaaaa ataattccac ttaaaaaaaa caacatcaac
    11461 aagtcaacag ctaatatctt gggagtggta agagaagtta taaaaaaggt tataggaact
    11521 atcaaaatgc aaactatgcc aaaaaatagc cattgaaaaa gaacaaaagg tgttttccca
    11581 ctcaaagaat acttatatgc aaacttagca taatcgttaa ttgctttatt caaggcttta
    11641 aatgatgtta ctttagcacc aaatatcttt ataacttgaa ttccgcggat atattctacc
    11701 gtttcagagc ttaacacatc taacgattct tgatattttt gaataaaatt tgtattctct
    11761 gtcattttct ttaacaagaa tacaccaatt actaacatgg cgattagaac caatccaatt
    11821 ttaaagttaa tgaaaaagcc taaggcaact gataaaacag gtgttactat ggcttgagca
    11881 ctatctggaa tcaagtgagc aattgccata tgtgttttag ccgcgttgtc atcaattgtc
    11941 tttcgaatat atcccgaaga atttaaatca aaaaaacgaa aacttgcttc tgttaaaccg
    12001 ctaattccct tttttctcaa attagtctct aagcgaaacc caaggatatg agaaaataag
    12061 ccagaaaaaa tataaaataa agctcctaaa gttaaccaag caacagtttg aaccgcataa
    12121 ctcttagcta aatattcatc tcctacaata attagagact gaagaaattt gtaaatactg
    12181 taatatccat agactgttaa tacgacagaa aaatcagaaa ataaagtggc taaccatccg
    12241 tagtatttca ttttagggac ataactaaat aattttttgt atactttcat tttttcctcc
    12301 cccgcattca atttcttctc ttattcgttt acaactgtta atgctttccg taatagcgta
    12361 aattagagat gtacaaacag ataaaaggta aactacgttc tattttttgt atttttttaa
    12421 gacttttctt ccaatcaata caccaatcat agataaactt gctacaacta acactgaaat
    12481 gattccccat atacctaccg catagtggat cattgcatcc attgttgcag cagattgtcc
    12541 tttatctatc caatcttgac gaaaacgatc tgtaaagaac catataggaa tccactgtcc
    12601 ggcagcactg cctaattgca ttagagccca agacgcaact tgaggaattg ctcgatcata
    12661 tccaaataca gatagaataa tatcagctaa gatcgccata ctaatcgaac tgataaacca
    12721 aggtaagtac gccccgccca ttaacgtaaa aaataacaat aaaacaattg tgtaaatagt
    12781 aaaaattcct tttctagggt agttatagct aatataaaca aatatgattc cagataataa
    12841 tccaaaaatc ccgggggata tgctgtgacc aaacgcccca ccaatactac taaccattat
    12901 tgaaaaaaag taaattacta gggaaataac tgtagctaaa ccaataaaaa tata
//
LOCUS       DASNNU010000070        12824 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.070, whole genome shotgun sequence.
ACCESSION   DASNNU010000070 DASNNU010000000
VERSION     DASNNU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.070"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1530
                     /gene="yfcC"
                     /locus_tag="U8116_002012"
     CDS             <1..1530
                     /gene="yfcC"
                     /locus_tag="U8116_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN8434996.1"
                     /translation="RYAYELDKSYVFDQLEELQDHPAEREKLDVPEKGLEKVIADGEK
                     NLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGGFGFLNFVFEGLV
                     SGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLERLALPLLFFAFS
                     FGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNATSWMSPFSVAVA
                     QGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLTYKSDDYFRSHIK
                     KTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFFVMGLVAGIIAVL
                     FKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASVPSALNTILHGIG
                     TALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADLVGVSRQVAVLAY
                     QLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLGTIFIMIAIAIGL
                     Q"
     gene            1687..2871
                     /gene="iadA"
                     /locus_tag="U8116_002013"
     CDS             1687..2871
                     /gene="iadA"
                     /locus_tag="U8116_002013"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEN8434997.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            2982..3185
                     /locus_tag="U8116_002014"
     CDS             2982..3185
                     /locus_tag="U8116_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN8434998.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3286..3948)
                     /locus_tag="U8116_002015"
     CDS             complement(3286..3948)
                     /locus_tag="U8116_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8434999.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(3961..5463)
                     /locus_tag="U8116_002016"
     CDS             complement(3961..5463)
                     /locus_tag="U8116_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8435000.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6011..7690
                     /locus_tag="U8116_002017"
     CDS             6011..7690
                     /locus_tag="U8116_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN8435001.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7817..8296
                     /locus_tag="U8116_002018"
     CDS             7817..8296
                     /locus_tag="U8116_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEN8435002.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8515..9195
                     /locus_tag="U8116_002019"
     CDS             8515..9195
                     /locus_tag="U8116_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEN8435003.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9203..10534
                     /locus_tag="U8116_002020"
     CDS             9203..10534
                     /locus_tag="U8116_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN8435004.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10549..11511
                     /locus_tag="U8116_002021"
     CDS             10549..11511
                     /locus_tag="U8116_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEN8435005.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11622..11960
                     /locus_tag="U8116_002022"
     CDS             11622..11960
                     /locus_tag="U8116_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEN8435006.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12039..12746
                     /locus_tag="U8116_002023"
     CDS             12039..12746
                     /locus_tag="U8116_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEN8435007.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4062 a         2268 c         2832 g         3662 t
ORIGIN      
        1 cgttatgctt acgagctgga taagtcgtat gtgtttgatc aattagaaga actgcaagat
       61 catccagcag aacgtgaaaa actggatgtt ccggaaaaag gattggaaaa agtcattgct
      121 gacggagaaa aaaatcttac gcaggaaaaa ctagatgaaa tctccttgac agatgatgtg
      181 ctttacgatc agtacggcga aaatatctat gatacttcta aaaaattgca taaaacagcc
      241 aaaatatggg gaacagatga ttttggcggc tttggtttct taaattttgt ttttgaagga
      301 ctagtatctg gagataagta tggatctgct gtagggatcg ctgcattgat ccttgttgtt
      361 ggaggtgctt ttggtattat tatgcgtacc ggagcgatcg acgcaggtat ctatgcgttt
      421 atcagtaaaa ctaagggact ggagcgttta gctttaccgc tattattctt tgctttttca
      481 ttcggtggcg ctacattcgg gatggcagaa gaggtaattc cattctcaat ggtgatggtt
      541 ccatttgtca ttgcgttagg atatgactcg atcgtagcag taaccgtgac ttatgtcgca
      601 tctcaagtcg gaaatgcgac ctcttggatg agcccgttta gcgtagcggt ggctcaaggg
      661 attgctggaa tccccgtgtt gtctggtgcg acattccgat tgattatgtg ggtagttgtt
      721 actgcattag ccgctggtta tatgatgata tatgcagaaa aaatccgtaa aaaaccagaa
      781 agttcgttga cttacaaatc agatgattat ttccgttctc atatcaaaaa gacatctgat
      841 gaaaataaaa aattcatgct tgggcataaa ttgatcttat tagagatgtt agttgtcctt
      901 gtctggatcg tttggggcgt gactcaaaaa ggctattaca ttccagaaat cgcttctcaa
      961 ttctttgtga tgggattagt tgctggaatc attgctgttc ttttcaaatt agatggaatg
     1021 aaagtcaatg acatcgcttc ttctttccaa tctggtgcag ctgatttagc tggtacagca
     1081 atcgtagtag gtatggccaa agggattctt cttgtcttgg gaggatctga tgcatctgtt
     1141 ccttcagctt tgaatacgat tttgcatggg atcgggacgg cgttgaccgg tgttccagct
     1201 gtcattggtg cttgggcgat gtacatcttc caaagtttgt tcaacttggt tgttacatca
     1261 aattcaggac aagcagcttt gactatgcca atcatggctc cactggcaga tttagtaggt
     1321 gtatccagac aagtagcagt tcttgcttat cagcttggtt caggttttat ggacgctttt
     1381 acacccgtat cagccagctt gattggtgtg ctaggtgttg cacggatcga atgggcaaaa
     1441 tgggcaagat tccaaatcaa gatgcaagga ttcctgtttg ttttaggaac tattttcatc
     1501 atgattgcca ttgcaatagg tctgcagtag tactggagac ctgtaaataa aaaagtaatc
     1561 caaacagtag ataaggactc caagaaccgt cattttacgg taaaatcaaa ggagtaaaga
     1621 tcctatcgtt tggattattt tttaagatcc tatcatcagt tatacttaaa gaaagaggaa
     1681 aaatgtatga aaatcattcg acaaattgaa gtgtttgcac cagattactt aggaaaaatg
     1741 gatgtactga ttgccggtgg gaagattttg gctgtagaag aacagctaga aggcggctac
     1801 gaaggagtgg aagtagaaga actttcaggt gagggaaaag tactaacccc gggtttcatt
     1861 gactgtcatt tccatatttt aggtggagga ggagaaggcg gttatcaaaa tcgaacgcca
     1921 gaagtcacat tgagccaatt gactacagct ggtgtaacga cagttgtcgg ttgtttggga
     1981 acagacggag aaggtagaga tatgactgcg ttgatcagca aagcgaaagg tctggaagca
     2041 gaaggaatct ccacttatgt atacgaaggt tcgtaccgat tgccggtaaa aacagtaaca
     2101 gattctatca tcaaagactt tttgacaatt gataaaatta tcggaatcgg agaaatcgca
     2161 gtatccgatc accgttcttc tcagccaagt tttgaagagt tcgctcgtgc agtagcagat
     2221 gctcgtgtag gaggcatgtt atccggaaaa gctgggatca tcaatgtcca cttaggcgga
     2281 ggaaaaagaa agctagagct attgacacga atcgtagaag aaacagaaat tccgatcaca
     2341 caatttttgc ctactcatgc aaatcgcaca ccagaattat ttgaagcttg cgtggcattt
     2401 gccaaacgtg gtggcacgat tgacttcact gctagcgaag atcctgattt ttgggagaag
     2461 acagatggtg aagttcgctt cagcaaagca ctgaaacgat tgatcgagga agatgtttct
     2521 ttagataatt tcacgatgac ctcagatgga caaggaagct tgccttattt tgatgaaaac
     2581 aatcatttcc tagggttagg agtaggaagt gccaaggcat tgctagtagg gatcaaagaa
     2641 gctgtgcaaa aagaaagtat ccctttggaa atcgccttac gagctataac cagtaatcca
     2701 gcacgtatat tgaaattgga taaaaaagga aaaatcgaaa ttggtgctga tgcagatctg
     2761 tgtatcttgg ataaagaaac gcttgatatt gatacagtta tcgctaaagg agaaataatg
     2821 gtccaagaaa aagaagtcaa agtatggggg actttcgaaa aaagttttta atcaacagct
     2881 ctaactatct tttttagaaa acgagcgttc tgtttgtctg tatctgttgt tgatgttacc
     2941 ctgaaggtag atcaataaac aaactaagag gaggaagcac aatggcagat ttaaaaggac
     3001 gttctaaaga tatgaaagat aaagtagtcg gtgaaggaaa agaagcatac ggcaaagcaa
     3061 cagacgacaa gagcaaagaa acagaaggga aagcacaatc actaggtgct gacatcaaag
     3121 aaaaagctcg aaatttaggc gacgaagtca aagacaagtt ttctcgaaaa gacgaaaaaa
     3181 aataaaaacc acttattact aattgaatca agcaaataaa aaatccgaac catatctgcg
     3241 agacagatat ggttcggatt ttttatttga tcattatcct gttttttaaa cttcgatttt
     3301 ttgaaatttt ttatttttta gttcaaagac ttcatcacag ctttttgcta cttcaatctc
     3361 atgagtcacg atgataatac agcgattttg ttcatgggcg atttcttgga aaagtttgac
     3421 gatatcttgt gaagttgttt catctagatt tccagtaggc tcatccgcta cgattagttg
     3481 atgttcacag cagatagcac gaacgatagc gactcgctgc tgctgacctc ccgaaagttt
     3541 ggtcacctgt ttttttgcta atatttcatc aattcctaca cgagccagtc gatctaaagc
     3601 aacttgtcga taattctctt gcttggtctt agtgatttcc attgctgcta tgacgttgtc
     3661 tagagccgat aaataaggaa gcaagttgta agcttggaaa ataatcgaga catcattttt
     3721 acgataactt cttaacccga ttttgttcag tgaacgatct tgatagtata cctttccttc
     3781 ttttggtgca tctagtccag aaataagcga aagaaaagtc gtttttccag aaccacttgc
     3841 tcctaaaata gcgtacattt ttccagcttc gaaattcagt tctacatctt tataaagcgc
     3901 atcttgcgga tcttgatacc aaaatcccaa ttgttctgtc cgtaacatca aattcctcct
     3961 ttagtttaat aggatttttc ttgggtttaa tcttaatatc cctatagaag ctaaaaagac
     4021 agaaaacaga ctgattccaa atgctactcc ggctaatagt cccaattgtg caggtttgac
     4081 attgatttcc aattcgctga cttgatcaga aacagcaaat ggattattcg tggttccttg
     4141 ttgaccatcg cgttgaccgc cttgttcttg tctgttacct ggcatttgtc caccgccagg
     4201 agcttgctgg ttttgtgtcg tggtttctgt ctgttggtca agtagctgct gtcccacggc
     4261 attcgcgacg acgtttccgc taagcgaagc aactcctaat gcaagaatca tacaaatagc
     4321 cacttcggta aacaattgga gaatgatttt acttctggat tctcctaaag atagcaagac
     4381 gcctaactca tgccgacgct cccgaatggt aatcataatg atcagagtaa gtatgataat
     4441 accggctaca gccactagta aaacaacatt tttagaaaaa cttgccacgt tgtttaaagg
     4501 ttccaacatt gactgataca tggaatcatt tgattgtaag ctaaatgtat ctgtgtcgat
     4561 cagcttctca gcttctttga gaaactcatc cattttatct ggatcagata gagtgtaaat
     4621 agcagaatct atcgtgttat cgtcagatgt cccatttagt tcatttgcaa aagtgtaaga
     4681 agtgtaaagt gtattcgacg gattcatgaa gttgaatttc atacccatac tgccgcttgt
     4741 ttctctggat tcataaattc ctttgatcgt catttcataa gtggtatctt catcttcagt
     4801 actcgtgatt gtgaaagtat ctccgactgc caggtcattt gtttctgcta gagtagaatc
     4861 aatgactaca ttgtttgtgc ctttgtcgct gtcatctatt ccttcaccat ccgtgatttt
     4921 tgctgttcca tcagaaaaat tactattttg tgcagttgct gacacaccac tgatttgaaa
     4981 gtctgcttga gtcatttgcg gattcccgcc cattcctcca gcaggttcac cgcctgcatc
     5041 agtagtttct gaactgtctt cacttgaatc agaactagtg atggcagtta ttccatcttt
     5101 tgccatagct gaggcggaac tttcaaagga ataactcttt acattatcta attcagctat
     5161 tttttccgcg tccgttaagg aaactggtgt aagagaaaaa ctgcctggat ctggacgttc
     5221 ttcagtggta gaaccgcttg aagaagtttg ttcctgtttt ttaaatgaag cttctcgatt
     5281 ggcacttaaa gtcactgttg ctccaacttc tttttgtgct tgattcgctg cttcttctgc
     5341 agcgcttcga atcgtgaggc cagctagcac aaaaataaga atggctgtga acacggcaaa
     5401 caataacaat gaacgtcccc attttacttt tgtacttttc catgcacgtt tcataaaatt
     5461 catattttgc atccctccta gtagatgcat ttgataatag acaacctatc ttaaagctag
     5521 cttaaatctt atgctgtttg cacaccatca acatgaggtt ctgtatatat aaaaagtcac
     5581 tatagttatt gcaaaaaatg tggtaaaatg aaaaaagtat gtttcaaacc gacgaagaag
     5641 aaatgaaaga ggaatagcaa aagaaatcct agatgctaca gagggatttt tacgtgcata
     5701 gttgttctaa caattgctga tactagcgat ttagtttatt tgttgtataa taatggggaa
     5761 cagctgaata cttatataag tattctggct atagcgtttc gcaaaagatc cattttgtta
     5821 atgaagtata tccaaaataa aatgcccatc caactgtcag aacgcggatg tgctggtaat
     5881 tttcctgctg attctccata agttgagaaa tagtgatttt gcatatgctt cattggaatg
     5941 gctcatatat ttagataaag aagaaaatcg acatctttaa acttggatgt cataagaaga
     6001 ggtgaagttt gtgagtacca ttaaaattat cccattaggc ggcgttcgtg aaaatggaaa
     6061 aaacatgtat gtcgcagaag tgggcgagga tatttttatt ttagattgtg ggctaaaata
     6121 tcctgaaaat gagttgttgg ggatcgacgt cgtaatccct gattttacgt atttagaaga
     6181 aaacattgat cgtgtagctg gtgtcttttt aacacatgga catgcagatg cgatcggggc
     6241 acttccttac ttgttgtcaa aaatttctgt accagtattc gggacagagt tgacgatcga
     6301 gttagcaaaa ttgaatgtca gtcgtaatga agcagccaaa ggatttaagg atttccatgt
     6361 ggtagatgag catacagaaa tagactttgg tgaaacagta gtgagctttt tccgtaccac
     6421 tcatacgata ccagattcag tggggatcaa tctgaaaaca aaagaaggta gtatcgttta
     6481 tacgggagat tttaaatttg accaaacggc tatcccaatg tatcaaacag attatgcacg
     6541 attggcggaa atcggcaaag aaggagtctt agccttacta agtgaatcgg caaatgcaga
     6601 aaatcctcag cctgtagctt ctgaagccca aatagcagat gaagtatttg atacgatcaa
     6661 atattgggaa gggcgtatca tcgtcgcttg tgtagcaagt aatttgcaac gagtgcaaca
     6721 agtattggat gcagcagaaa aagcacaacg aaaagttgtc ttgactggtc aagatttcga
     6781 acgaattatt cgcacagcga tgcgtttaga aaaacttcaa ttaccaagtg aagatctgct
     6841 tgttacgttg aaagacatga aaaactatga tccagaagaa ctgatcattt tagaaactgg
     6901 ccgaatgggt gaacccatca agtctctgca aaaaatggca aacggtacac accgtaatgt
     6961 caagattgaa gaaggagatt tggtttatat cactactacg ccaagcatcg cgatggaaac
     7021 catcgtggcg aaaacggaag atatcattta tcgagctggt ggaacagtga agttgatctc
     7081 tgagaatatg cgcgtttctg gccatgccaa tcctaatgac ttgcaattaa tgatcaattt
     7141 gatgaaacca acatattttg tgccagttca aggagagtat cgtgaattag ctgctcatgc
     7201 agatttggct catgcagtag ggatgccata caaaaatatc tatattactg gacgcggaga
     7261 cgtgctggaa tataagaaca agcgtatgag tgttgcagga actacaagtg cagaaaatgt
     7321 aatgattgat ggattaggca taggtgatat cggaaacatt gttttacgtg atcgaaaaat
     7381 cttgtcagaa gacgggattt tcgtagcggt tgtcactatc agtcggcgag aaaagaaaat
     7441 catttcaaaa cctcaaatta cttcacgggg attcgtttac gtaaaagcta gccgtgatct
     7501 gatgaaagaa agcagcaata tgattgaaac aatcgttgag aaacatttag aaagcaatga
     7561 ttttgagtgg agcaaattaa agcaagacat ccgagatcaa ttaggccgtt acttatttga
     7621 acaaacaaaa cgccgcccag tgatcttgcc agtcattatg gaagctactc aacgtcgaaa
     7681 taaaaaataa acaaaaagag taagatgaac ctctctatag agtgctcgtc ttgctctttt
     7741 tttgcgaaaa acaaataatt tttgaataca gcatccctcg ctttataata aacgatagaa
     7801 taaagggagt gaatcaatga tgagtcaaac aaaagtcgaa cgttatttag aagaacaaaa
     7861 gatcagctat caaccacttg attttagtca tgtggtagaa gaatctttca cagaagaact
     7921 acaaaaaaga gggattgatc cagctttgat atgtaaaact ctcgtagtga aaggaaacaa
     7981 aactggggtg atcatcgcat tgattccttt gaacgatcac ttagattaca aaaaaacgag
     8041 acaagtgacg aaagatcgta aagttggttt tcctggaatg gattttgtat taatgcatac
     8101 aggatatccc cacggagcaa acacccctat tggaataaaa ctcgcacatc ctgattattg
     8161 ttttttagca gacagttctt taaaagaaaa agaagaagta attgtgtcaa gcggtgaaat
     8221 cgggagaagc cttcggataa aagtagccga tttagaaaag atcgtccatc ctatttacgc
     8281 tgatttaatt agttaaggga cgagaagaga agctaagcag agcagagacc tcaaacttga
     8341 gattatatct gcttggtact tggtgcatcc tgtattatct gttacaattg ctttgtacat
     8401 actgtactat caatccaatg aaaatatatt tagcagctat attttttcat tgatgtgcaa
     8461 gtgatgaaat cgtttttaat aaaaaagata cagagaaaac gcgaggtgta aaatatgaaa
     8521 attacgtatc atggacattc ctgtttaaca attgaaatgg aaaatggcca acgcgtgatg
     8581 attgacccgt ttattacagg aaatccattg acagacttaa ctgcagaaga agtaaagaca
     8641 gattggttgc tgattactca tggtcatagt gaccacatag gagatatgat tcctattgca
     8701 aaagaaaatc aagccacaat tatcagtatt gttgagattg ctgattatgc gaacagcaga
     8761 ggagtagatt cattcggtat gaatatcggt ggaaaacatg cgtttccatt cggcgtggta
     8821 aaattcgtac atgcacagca tagttcaagc tatgaagtag acggtatcgt tcagtatatg
     8881 ggcgagccta gcggaattat tattcaagca gaaggaaaaa cgatttatca tgcaggggat
     8941 actgcttatt tcagtgatct aggattgctt gcagaagaat tcgatattga tgtcgctttc
     9001 ttgccgattg gcgataacta tacgatgggg ccagaagatg cagcaaaggc tgcgcaaaca
     9061 attcgtgcca agaaagtagt tccaatccat tacaatactt tccctgtcat tgaacaagat
     9121 cccgaatctt ttgtagagtt actggaagat agtgaaggaa aaataatgag cgtctcggaa
     9181 acgctggaag tgtaggcaga aaatggcgac aaaacatgat ttgattttag aatatattga
     9241 aagtttacct gttggcaatc gaatttctgt aagaagcatc gcaaaagaat tgaatgtcag
     9301 tgaaggaaca gcgtaccgtg caatcaaaga tgcagaaaat gtcggactag tttcaacaat
     9361 tcaacgagta gggaccatcc gtatcgaacg caaattaaaa aaacatattg aacgtttgac
     9421 atttggcgag gtcgtcagga tcattgaagg ggacgtttta ggcggggcgg caggattaga
     9481 caaggtcttg aataaattcg tcatcggcgc gatgactaaa catgccatgg aacgctatat
     9541 tacgccagga tcactaatga tcgtcggcaa ccgggcggaa gtccaaaaat tagcattgga
     9601 agatggcgcc gcagtattga tcacaggagg attcaatacg actgctgaga ttgcaaagct
     9661 agctgatgat cttgaaatgc cgatcttacg cacctcttat gatacattta cagtcgcaac
     9721 catgatcaac cgagcgttaa gcgaccaatt gatcaaaaaa gatatcatgc ttgtcagtga
     9781 tatctatatg ccagctgaaa agactcatta tttgcagcag acagatacga tttctgatta
     9841 tcagcgtttg tcagaagaaa cgcagcattc gcgttttcca gtcgtcaacc gccatcatcg
     9901 tttgatgggg attgttacgg caaaagatgt tttagggaaa tcgccaaacc agatcatcga
     9961 tcgggtgatg acgaaagaac caatcagtgt aaagaaaaca atgagtattg cctctgttag
    10021 ccatcaaatg atctgggatg ggctagaagt catgccggtc gtttccgatg acttgacatt
    10081 ggaaggcttg attacccggc aagatgtaat gaaagcgatg cagcttgtcc agcgacaacc
    10141 gcagattgca gatacgattt cggatcagat atctggaaat atccatacga ttgatactga
    10201 ccgtgaagga aatcgtttgg aaaaccctaa gttcaaattc gtagtagcac cacaaatggt
    10261 caatggcgtt ggaaccattt cttttggcgt acttagcgaa atcatttctg atgtcgcaca
    10321 aaaaacaatg gtgatgaacc aaaaacgaaa tatcttgatt gaacaagtga acctacatta
    10381 tttgcggctg atccaattgg aaagcgaatt agacattcgt ccacgtgtat tggaaatcgg
    10441 cagacgttcc gctaaactgg atatcgaagt gtttattgaa aacaccatcg tttccaaagc
    10501 aatcgttgtt tgtcaagtaa tggaacgttc ttagaaagca ggagtgaaat gacagtacag
    10561 caagaaatag tagatacaat aaaaaaatac gataaaatca ttatccacag gcatttgcgc
    10621 cctgatcctg atgcattagg atcacaagta ggattagccg aactattacg ggaaagtttc
    10681 ccagaaaaag aaatacgtca agtaggtgaa acgattgaag ggcttcgctt tttagcagaa
    10741 atgcaagaag ttgaagatgt cttctaccaa ggagcgctag tcatcgtgac tgatacagct
    10801 aattcaccca gaatcagtga tcaaagatat cagttggcag aaaaactgat caaaatcgac
    10861 catcatccca atgatgaccc ttatggagat cttgtgtggg taaatacaaa agcaagtagc
    10921 tgtagtgaga tgattgcaga atttgctttg atgtttcctg aatctttaaa gatgaacgtt
    10981 aaggctgcac ggttacttta tgcaggaatc gtcggagata cagggcgctt tttgtatccc
    11041 tccacgacgt cccgtacatt agagattgct tcgatattga gaaattatcc atttgatgca
    11101 agtgctttga acagagagat tgaacaaatg ccgatgaaag tcgctaaact ttccggttat
    11161 ctttatcaaa atatccaggt ggatgaaaat ggtgcaggaa gaataatcct tccgcaagaa
    11221 attcttgata aatatggaat cgatgattca gagacagctt cgatcgtttc acttcctgga
    11281 gtgattgacg aagtgctggc ttgggggatt tttgttcagc agccttcagg gtattaccgc
    11341 gtacgtttac gttctaaggg gccgatcatc aatgaattgg ctaaacgcca tcatggagga
    11401 ggccatccat tagcaagtgg tgcgaatgca aaagatttag cagaagtaga ggaaatctat
    11461 aaagagatcc aagatatatg ccaatcgtat aagagcagaa aaacagaatg atacttcgtt
    11521 cgaaaacgtg ccttttgtag taaaaatcac aaatgtatct tttcaagatc ttctttttcc
    11581 gttacaatag tgctcactaa agattttaaa ggagaaagaa gatgacatta ccaaattgcc
    11641 cagaatgtgg ctcagagtac acatatgaag atcgaggctt gttgatttgc ccagaatgcg
    11701 ggaacgaatg gaacaaagaa gaacaagaaa ctgtcataga agacggatta gtggtattag
    11761 atgcaaatgg aaaccgtctg ttagagggcg atagcgtgac agtgattaaa gacttgaaag
    11821 taaaaggtgc gtcaggtgcc attaaacaag gaacaaaagt caagaacatc cgtttagtag
    11881 aaggcgatca taatatcgat tgcaaggtag aaggatttgg accgatgaaa ctgaaatcgg
    11941 aatttgtgaa aaagaattaa agaatattca aggtcttttc acattctttt cctactacta
    12001 atgataaaat aaagtaaacg aaaaggagtg aaacgaaaat gtatggttat ggaatgtatg
    12061 gttttttaga tccgacattt cttttggtca ttcttggatt agttatttcc ggaattgcgt
    12121 cagcgtacgt caatagtact ttccgaaaat acgatcaagt gagaagcaaa aacaatgtga
    12181 ctggtacaca agcagcacaa tatatattac aaagtcaaca gataaataat gtcggtgttc
    12241 aacaaattgc cggagatctc acagataact acaattcagg taataagatg ttgagtttgt
    12301 ctcaagcaac tgcacagtct acttcagttg ctgcgatagg tgtagcagct catgaatgcg
    12361 gacatgcggt acaagatgct tcgggttatg tcccattgaa gattcgtgca gcacttgttc
    12421 cagcagctaa ctttggttcg atgatttcgt tccccttgat tatggtaggt gtattgctta
    12481 gctggaactc tacgttaatc aatatcggga ttttcgcttt ttctttagca ttacttttcc
    12541 aattggtgac attaccagtc gaatttaacg cgtctcggcg agcaatccaa attttgagtg
    12601 aaggcggact actgacggaa gaagaagtac cgatggcaag acacgtatta tttgcagcag
    12661 cactgactta tgttgcagca gcgctttcga cattcctgca gctgttgcgc ttgatcttat
    12721 tgttcggcgg caaccgaaga gattaaaata aagaagttgt gaaatcagcg ctttgatctg
    12781 agctaatacc gagagttagc tgattgaata ccatttattt ggaa
//
LOCUS       DASNNU010000071        12747 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.071, whole genome shotgun sequence.
ACCESSION   DASNNU010000071 DASNNU010000000
VERSION     DASNNU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.071"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U8116_002024"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U8116_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN8435008.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="U8116_002025"
     CDS             complement(323..892)
                     /locus_tag="U8116_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEN8435009.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="U8116_002026"
     CDS             1078..1980
                     /locus_tag="U8116_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN8435010.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U8116_002027"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U8116_002027"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEN8435011.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U8116_002028"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U8116_002028"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEN8435012.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="U8116_002029"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="U8116_002029"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEN8435013.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="U8116_002030"
     CDS             8554..8739
                     /locus_tag="U8116_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEN8435014.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="U8116_002031"
     CDS             complement(8801..9997)
                     /locus_tag="U8116_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN8435015.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="U8116_002032"
     CDS             complement(10167..11603)
                     /locus_tag="U8116_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN8435016.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="U8116_002033"
     CDS             11896..12705
                     /locus_tag="U8116_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN8435017.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DASNNU010000072        12601 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.072, whole genome shotgun sequence.
ACCESSION   DASNNU010000072 DASNNU010000000
VERSION     DASNNU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.072"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(324..893)
                     /locus_tag="U8116_002034"
     CDS             complement(324..893)
                     /locus_tag="U8116_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN8435018.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="U8116_002035"
     CDS             complement(967..2256)
                     /locus_tag="U8116_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435019.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="U8116_002036"
     CDS             2610..3458
                     /locus_tag="U8116_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEN8435020.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="U8116_002037"
     CDS             3472..4545
                     /locus_tag="U8116_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEN8435021.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="U8116_002038"
     CDS             4535..6706
                     /locus_tag="U8116_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEN8435022.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8974)
                     /gene="copB"
                     /locus_tag="U8116_002039"
     CDS             complement(6818..8974)
                     /gene="copB"
                     /locus_tag="U8116_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEN8435023.1"
                     /translation="MHMGHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHMGHNHG
                     DIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGMEFPFQ
                     VTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYIYSVYS
                     FIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPESVKRL
                     TKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGIKKQAN
                     DKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAKWLFYV
                     ALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAAKNGLL
                     LKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGALEANAN
                     HPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLGLNVEQ
                     ALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVMLTGDNK
                     NAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLARASIGM
                     AIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILAIPLAA
                     GILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9117..11303)
                     /locus_tag="U8116_002040"
     CDS             complement(9117..11303)
                     /locus_tag="U8116_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEN8435024.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="U8116_002041"
     CDS             complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="U8116_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEN8435025.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11525..11965)
                     /locus_tag="U8116_002042"
     CDS             complement(11525..11965)
                     /locus_tag="U8116_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEN8435026.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12039..12512)
                     /locus_tag="U8116_002043"
     CDS             complement(12039..12512)
                     /locus_tag="U8116_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HEN8435027.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
BASE COUNT         3537 a         2653 c         2235 g         4176 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgtcccatgt gcatggaatg gtccatctgg ctatggtcat
     9001 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     9061 tatgttcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     9121 tttttcatgt gccgatttaa gctcagggag ttcaacaata cactgacgga actaaacgcc
     9181 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     9241 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caccctagac
     9301 aactcgattg tttgtgcaat ggatgccaaa ctgctactca tcagcgtcac atcggctgtt
     9361 tccatcgcga tatccgttcc acttcccatt gccataccaa catcagctaa tgctaaagcc
     9421 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     9481 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     9541 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     9601 tctaatcctt tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca
     9661 gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     9721 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     9781 aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca
     9841 cctgggtgag ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     9901 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     9961 aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt aatcgtccca
    10021 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
    10081 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct
    10141 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
    10201 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
    10261 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
    10321 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgtg
    10381 acttccatgt gtagcagacc attcgtattg attgtcccgc caaaaagcgt gtcatccgga
    10441 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
    10501 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
    10561 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
    10621 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
    10681 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
    10741 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
    10801 ccaattgcta ccaatacatc catatttggt gctttcgact tgattgcatg gtacgcacct
    10861 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
    10921 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
    10981 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
    11041 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
    11101 tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
    11161 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
    11221 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
    11281 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
    11341 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
    11401 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
    11461 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
    11521 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
    11581 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
    11641 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
    11701 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga
    11761 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag
    11821 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
    11881 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactaatct
    11941 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
    12001 aacttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt
    12061 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
    12121 cttgcattag ctacttggat cagctgctgt atttttggtt cattgtttcc ttcaaaatgc
    12181 cagataaaag ataaaaattc taagtatttc ctgtttagtt tctccgaaaa tgattcaggc
    12241 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
    12301 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
    12361 tctaacgtag aagtataatt tccttcaacg atccatgacg gattagatac cataaacgat
    12421 tgctgttctt gcaaaaacca cttttttgcc tgaatgcgga aagccggtga tttctttgat
    12481 cttccttgcg agggacgatt ttcccgaccc actttgtcca atcacgatga tcttcattct
    12541 tctccctcac ttctctttgg aatttggttc gatcatacca cttataacgc cgcaaaaaaa
    12601 c
//
LOCUS       DASNNU010000073        12290 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.073, whole genome shotgun sequence.
ACCESSION   DASNNU010000073 DASNNU010000000
VERSION     DASNNU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.073"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(364..1482)
                     /locus_tag="U8116_002044"
     CDS             complement(364..1482)
                     /locus_tag="U8116_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435028.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1953..3260
                     /locus_tag="U8116_002045"
     CDS             1953..3260
                     /locus_tag="U8116_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN8435029.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3379..4578
                     /locus_tag="U8116_002046"
     CDS             3379..4578
                     /locus_tag="U8116_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN8435030.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4605..5873)
                     /locus_tag="U8116_002047"
     CDS             complement(4605..5873)
                     /locus_tag="U8116_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEN8435031.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6036..6682)
                     /locus_tag="U8116_002048"
                     /pseudo
     CDS             complement(6036..6682)
                     /locus_tag="U8116_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7090..7593)
                     /locus_tag="U8116_002049"
     CDS             complement(7090..7593)
                     /locus_tag="U8116_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN8435032.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7716..8480)
                     /locus_tag="U8116_002050"
     CDS             complement(7716..8480)
                     /locus_tag="U8116_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEN8435033.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8588..8863
                     /locus_tag="U8116_002051"
     CDS             8588..8863
                     /locus_tag="U8116_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEN8435034.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9301..10251
                     /gene="yidC"
                     /locus_tag="U8116_002052"
     CDS             9301..10251
                     /gene="yidC"
                     /locus_tag="U8116_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN8435035.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10430..10630)
                     /locus_tag="U8116_002053"
     CDS             complement(10430..10630)
                     /locus_tag="U8116_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435036.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
     gene            11435..>12290
                     /locus_tag="U8116_002054"
     CDS             11435..>12290
                     /locus_tag="U8116_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HEN8435037.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSIDFRE
                     VNQTFISSV"
BASE COUNT         3743 a         2558 c         2175 g         3814 t
ORIGIN      
        1 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
       61 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat
      181 gaagagctat ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      241 ctcatacacg gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg
      301 tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct
      361 cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa
      421 aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg
      481 ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat
      541 aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat
      601 gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga
      661 cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat
      721 cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc
      781 tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta
      841 cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt
      901 tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa
      961 accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg
     1021 tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt
     1081 caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata
     1141 aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt
     1201 ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc
     1261 tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt
     1321 ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac
     1381 atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc
     1441 tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa
     1501 aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa
     1561 acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta
     1621 tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga
     1681 taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt
     1741 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca
     1801 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
     1861 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
     1921 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
     1981 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
     2041 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
     2101 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
     2161 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag
     2221 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
     2281 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
     2341 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
     2401 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
     2461 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
     2521 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
     2581 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
     2641 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
     2701 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     2761 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     2821 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     2881 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     2941 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     3001 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     3061 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     3121 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     3181 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     3241 ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     3301 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     3361 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     3421 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     3481 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     3541 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     3601 ttcacagaaa aacgaaggct actgaatggt cttttattca atgttttact catcagtttc
     3661 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc
     3721 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     3781 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     3841 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     3901 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     3961 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     4021 cgatacaatc aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt
     4081 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     4141 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     4201 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     4261 cttgtcgaaa ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg
     4321 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     4381 agagccggtc tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     4441 gctttctact acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac
     4501 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     4561 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     4621 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga
     4681 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     4741 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     4801 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     4861 tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     4921 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     4981 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     5041 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     5101 cgacaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     5161 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     5221 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     5281 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     5341 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     5401 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc
     5461 aatcggaaca aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct
     5521 tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga
     5581 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     5641 ccacgactag aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     5701 ggtagtagac aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     5761 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     5821 cacttcagca tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat
     5881 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     5941 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     6001 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     6061 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     6121 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     6181 aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     6241 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     6301 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     6361 gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc
     6421 tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt
     6481 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta
     6541 atatgtctcg aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat
     6601 tgacagatga gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt
     6661 atggttttat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     6721 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     6781 ataagatacg ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg
     6841 attcattcga taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa
     6901 aaaggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag
     6961 ctggcatctg cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat
     7021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg
     7081 ttttatctat taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact
     7141 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     7201 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     7261 atctcgctca agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg
     7321 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     7381 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     7441 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     7501 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     7561 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     7621 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     7681 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     7741 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     7801 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     7861 attgcgaagt tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct
     7921 gtgccataca ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt
     7981 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     8041 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     8101 atcatcgtcc ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa
     8161 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     8221 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     8281 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     8341 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     8401 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     8461 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     8521 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     8581 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     8641 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     8701 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     8761 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     8821 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
     8881 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
     8941 ggacttaatg ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc
     9001 tttgcctcaa aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca
     9061 tttgactccg ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac
     9121 acttggaatc attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa
     9181 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
     9241 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
     9301 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
     9361 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
     9421 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct
     9481 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
     9541 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
     9601 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
     9661 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
     9721 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
     9781 acagaaggta tccgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
     9841 cttgttgcca ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt
     9901 cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc
     9961 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    10021 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    10081 gcagaagagt tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaat tgatgtgaca
    10141 gaatcagcta aaccagctga cagcaaacaa gcaaccaaac aaatcaagca acccaaaaac
    10201 agtacaaaca ataaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat
    10261 aagagtgtaa taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt
    10321 gaaaaagaaa tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg
    10381 atcgctatct aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa
    10441 cgtagacagg tgttgctgtt tcttctaata gtgtacttaa ttccttaata ttttgttttc
    10501 cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa ggcgccactc
    10561 ccttcgccca tgttgctcgc ccacataaga caccattctc cataaaggac aacgcggaga
    10621 taccacccat ctaaataaca tccataaacc tttctagtag aagaatagtg atagtatcca
    10681 tccctttttc tactgggaat acaattacac gattcaaagc ttgatacaca acatttccct
    10741 tcttataaac cgctattgta accgaattgt tttgaatcac ctttttggga tagaaactgg
    10801 ctcctttccg caacttctta aatgttggtt tcttttatac tttctttgta gcaactacaa
    10861 atttcttcaa atctctaaat gtttgcataa caaccgcctt tctgactttt actcaggatg
    10921 gtatttaaaa atcagcaaaa taaggcttct ctccatcttt tttatttcct agctgcttta
    10981 aagacactct tgctgttttt taatctctat caagggggct ctgaatctat tttgatgaac
    11041 ttatcttatt aatttctcaa atgagctgtc agacacaact gtattttttc aatctcatac
    11101 cggacaacta tgccagctta aaaaaatttc ctctggcaaa acttctttat tgaaaaatga
    11161 caatgaaaaa agaccattct ttttgttgaa tgctcattgt gtaaacatat ttcctgcgac
    11221 tgctcttcga accattctca gtgcaatccc taatttatct attaaatctt cttttagttc
    11281 ctcatctttc atattcaaaa tttcctttgt atgttacaca agtttgttct ctaaataacg
    11341 cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc aaattgtatt
    11401 tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc ttaccataga
    11461 cgaactgaca atgatagaat catattatct tcaacataat aagccggttg aaatcgctaa
    11521 ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca agcaaggcaa
    11581 gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg gtagaaaagt
    11641 cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc ttggttggac
    11701 gcctgacgtc attatcgggc gaaaagaaag gtctgtttca tgcggtatga gaacacttta
    11761 tcgtttattt tctaaaggaa tatttgatat tgacacacta ccgatgaaag gtaaaagaaa
    11821 acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa tccatgatcg
    11881 acctgataat tatcctgatt tcaattctga gtttggtcac cttgaaggtg atacgatcgt
    11941 tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta aagtcattat
    12001 cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta atcaatggtt
    12061 ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa aagaattttc
    12121 taactggaaa gccattagta accaacatga tattgatata tattttgcgg accctggaac
    12181 accttctcaa cgcccattaa acgaaaattc taacgggatt ctgcgtcgta atggactgcc
    12241 gaaatcaata gattttagag aagtgaatca gacatttatt tccagtgtca
//
LOCUS       DASNNU010000074        12233 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.074, whole genome shotgun sequence.
ACCESSION   DASNNU010000074 DASNNU010000000
VERSION     DASNNU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.074"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(59..1042)
                     /gene="istA"
                     /locus_tag="U8116_002055"
                     /pseudo
     CDS             complement(59..1042)
                     /gene="istA"
                     /locus_tag="U8116_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="U8116_002056"
     CDS             complement(1437..2279)
                     /locus_tag="U8116_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN8435038.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="U8116_002057"
     CDS             2604..3020
                     /locus_tag="U8116_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN8435039.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="U8116_002058"
     CDS             3017..3487
                     /locus_tag="U8116_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN8435040.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="U8116_002059"
     CDS             3501..4274
                     /locus_tag="U8116_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN8435041.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="U8116_002060"
     CDS             4288..5100
                     /locus_tag="U8116_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN8435042.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="U8116_002061"
     CDS             5154..6119
                     /locus_tag="U8116_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN8435043.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6255..8978
                     /locus_tag="U8116_002062"
     CDS             6255..8978
                     /locus_tag="U8116_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN8435044.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8991..9623
                     /locus_tag="U8116_002063"
     CDS             8991..9623
                     /locus_tag="U8116_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN8435045.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9828..10055
                     /locus_tag="U8116_002064"
     CDS             9828..10055
                     /locus_tag="U8116_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEN8435046.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10124..10390
                     /locus_tag="U8116_002065"
     CDS             10124..10390
                     /locus_tag="U8116_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN8435047.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10380..10649
                     /locus_tag="U8116_002066"
     CDS             10380..10649
                     /locus_tag="U8116_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN8435048.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(10828..12129)
                     /locus_tag="U8116_002067"
     CDS             complement(10828..12129)
                     /locus_tag="U8116_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN8435049.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         3989 a         1984 c         2400 g         3860 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcagatgcag cgattaaatg actatcttta tcaactaaca ttgcttttga
     6301 aatctacgcc ttcagataat tattcagctc gcagtatttc gcaagctcta cagttgaata
     6361 gaagtacaat cagtagctac ttaaatgaag gtgttcgtga agggcttttc ataaaagtaa
     6421 aaagttaccc ggttttattt ttacatagaa cagctcttga agaactacat atcacactga
     6481 ataactccga aatagaatca attgaaagct tgttaacaat ttctcaaaaa cccgcattag
     6541 atcaagtaat tggctctaaa ggaagtttga aagaagcaat tgatcaaatt aaaacggcag
     6601 tcttatatcc tggaaaaggg ttgcctttat tattgatcgg cgctagtgga tcagggaaaa
     6661 cctttctagc gaataaaata tatgaatatg cagtagaaga aaaggtcatt cacacatcgg
     6721 cacctttcat agattataat tgtgcacaat atgtgagcaa tccagagtta ctatcctctg
     6781 ctttatttgg ctacacgaaa ggagcattta ccggagcaag ccaggagcat actggtttgc
     6841 tagaaaaagc cgatggagga gtattgtttt tagatgaagt gcatcgctta tctgaagaag
     6901 ggcaagagaa attatttaca tttatggata cgggtgaatt ctctcctatg ggggataata
     6961 gtattcggaa aaaagccgat gttcgattag tctttgcaac aacagaaaat atatatacga
     7021 ctttcttacc aacttttctt aggagattgc cagttattgt taatctacct agatttcaac
     7081 aacgtccgtc gtttgaacgt ctgtcattga ttgatgaatt ttttgtttct gagagccaaa
     7141 ttttagctaa ggaactatct gtttctgatg cattgattca tttcttaatg aatagtgatt
     7201 ttgaaggtaa cgtggggaat attaaaaata ttatcaagta tgcttgcgga aacgcatata
     7261 tccaccagaa gagtcagcaa acaattcaag ttaaattatt ggatatgcct ttagagtaca
     7321 gttataaatt caaggagcaa ctaaataaac ccagaaagaa aagagcagat cgaatctact
     7381 atccagaaga aacacgacaa gtgcaactag aaagaaacaa tcagcttttt gttcaatttt
     7441 ttgaccaatt acttactgga ttctctgaag tgattgaaaa taacaaggat attaaactat
     7501 ttttagaaga aatggttacc aaggtaacac aagtaatgga tacgcttatt tttcaagagg
     7561 attacgaaaa agagcagtct ttatacgcca ttctaaacta tcatattcgt caaagtattg
     7621 attttatgcg agaaagctac gggtttgaac aagatggtaa ccgggtagtc gctttggcca
     7681 actatctata tgtcaaggat aatcaagtga tttttgaaaa tagtcttcaa gaaaagaaaa
     7741 ccaaagatca gcttaacgaa ttttttgcta atgttcttga aaccccttat tggtatgcaa
     7801 aaaaaatctt aagcctttta tctgagtgtt tagatcaacc tgtgtatgaa gaggacattt
     7861 tctttattac gctatatttt tatagtttga atgtcacgaa tgttaaaaca ggtattaaga
     7921 gtattgtgct agctcatggt tattctacag ctagtagtat ggcgaatgtt gccaaccgta
     7981 tgctgaataa gaacgttttt caagcttatg atatgccttt agatattaca ttagagaaaa
     8041 ttgaagcaaa attgattcaa ttccttggag attatcgaac agattcaggc ttgattttat
     8101 tggtagatat gggttcacta aaccaactag gcggtttatt agtgaatcat ctaaatggac
     8161 cactgttaat cattgatcat atcaatacac cgttattatt ggaagtcggc caacaagtat
     8221 taaacaataa aagtatcacc gaaataggag cagaaattga aacaagtctt actttcaaaa
     8281 agcagttact acttcccaag aaaaagaaaa agaaagccat cattacttgt tgttatacag
     8341 ggattggtag tgcgacccag attcaggaga ttttaacgaa atgcttgaaa gatgcagtta
     8401 gagaattagt aattattccg tatgattaca aaaagctgtt agaaaacaag aattttgaga
     8461 ctccttttca attatacgat gtcatcatga ttgtaggtac agaagatccc aaaattaatc
     8521 agattccata tattggatta gatcgcctga ttaatggggg aagcattggc gaatttgttc
     8581 attacttgga aaaagagctg actattgaag tgaacgattt acaaaaagaa ttaattttca
     8641 atttttcaat tagtaaaatt gtcgaaaatt taaccatttt ggatccagat aaggtgctat
     8701 cttttgtaca aaaagcaatt aaagaattat cgaagttatt aaattttgat ttttctaata
     8761 atcagctgtt tttgttgtac cttcactgta gttgtatgat tgaacgaatt gtacgtaaag
     8821 aaagtgtgga tgagcaagag gacattgaag aatatatgca agcaaatgga gaaataatgg
     8881 ataaaattca tagcgctttt tttgagattg aaagggaata cacaattgaa ataccagatt
     8941 tagagttgcg attattgagc gatattgttc aaggatagga gtgaattcga atgacaatgg
     9001 gagtaatttt tgatatggat ggggtactca tagatagcga gacattttat tttgagagaa
     9061 gaatgaactt ctttcaagaa ctggagatag atccaggatc aaaccagatt gatgattatg
     9121 tggggaaaac agaagctgca atatgggaaa cattagttcc tgaggacaaa tcattaagag
     9181 aggaacttta tccgaaatat gtagattatc gaaaaacgca tccaattgat ttcgctaaag
     9241 ctttacgaaa agaagtgaaa ccgttacttg acttcctcaa gaagcgggat ataccattgg
     9301 cactggcatc ttcttctcca gctgtcgaga tagagagtat gctcattcaa tgtgaactta
     9361 ctgaatattt ttcctttgtt ataagtggtg agtcattaaa acaaagcaaa ccacatcccg
     9421 aaatatattt aaaagcgaaa cagcaattag actgtgactt ctttattgca gtagaggatt
     9481 caacgattgg tattaaatca gcaaaggcag caaatatcta tacggcggca ctaaaacaac
     9541 caatgcaaat cgatcagagt gaagcagaca tacaaattga aagtctacaa gaactggaac
     9601 aaattgtttt agaaaaaggt tagtgaatta atggaaataa ggctatatat ctgtgctagc
     9661 tattttgact aaccaaaaaa acttgctata attcaggtgt agtaaaaggt gctggtaaca
     9721 ccttttacga aaatgcttcg gcagtgataa tgggattggc tttttaagtt ttttattttg
     9781 ttcgcaatcc ctaaggtcat gtgaatgatc ctagggattg ctagaatatt ttgcaacaga
     9841 accaaaatac gaatagttat caagcagcca tattgactgc ggtcaattta ggaggggata
     9901 ctgatactac tgctgccgta actggtgctc ttgcgggtat tctttacggg ggtgaagtga
     9961 tccctgaaaa ttggagaaaa tcgcttcaaa atgaccagct tcttaaaacg atcagtcaga
    10021 gattttgcga taccgtgcta tctcaaagaa tttgaataat ggatatgatg caaatcttgt
    10081 ttgtactaac gttgtattga caaaaaggat tttatttagt acaatgttag tacaaatgat
    10141 gaacggaggt agaatcatgt ctcaatcaac cattacattt aggattcccg acgatgaaaa
    10201 agaactggta ttagaatatg caaaagcaca caattcttca ttgaccgaac tttatcgaaa
    10261 tgctgtactg gataaaatcg aagatgaaat cgatctacgg actttacgga gtgcaattga
    10321 agtttccaaa gagaaaaaag agagtggtat ttctcaagag gaaatggaga aactgctaaa
    10381 tgaattataa agtcatctac atgccgcaag cccagaagca attaaaaaaa atggacagaa
    10441 accaagctaa aataattgtc gcctggatca gaaagaattt agaaaacacg actgacccgc
    10501 gtcagcacgg aaaaggtttg actggaaata agagtgggga atggcgctat cgaattggaa
    10561 actatcgaat attggctaat atttgtgatt ctgaaattga aatccagata ttctcaattg
    10621 gacatagaag tacaatatat aaaagataac cgtttttgga acgaagtaga gtttcgaaag
    10681 tcaatgtata atcttttgaa ttttgcataa taccacaggt agaaactacc cgatactatc
    10741 ggttggtttc tgctttttta tggctctttg tcaaatagga ctgatagagt gagataagaa
    10801 catctaaggt agaattcctt ttctacttta gatgtttttt gattcatagg taatcagatt
    10861 atttagtaag aaaattctgg tcttcatttt ccgaaagctt cgaaagccat aagcgactcg
    10921 tttcaatgct ttgatgtggg tatgcagatt ttctattttg gcatttgaat aaggcaattc
    10981 gagggctaaa cgaatgccgg attcatagtt tataaaagct ttttttatag aatgcagttc
    11041 ttttggaacg gaattgggca tcgttcttac aagtttaaag aaagtgtccg cctctttgtc
    11101 gtgaaacgct tgaagaattt cttgatagta aaaataggcg attcgtaatt cgtcagagat
    11161 cgaaagcaga cgatccacca cttcactttc ggtgagatac gcccaaccaa aattgcgcca
    11221 tttggtccat ttgtcataaa caagcttgtc ctgtcgtttg atcagaagcc gagacaacgc
    11281 ctttaattgt cgataatatt tggcttgtgt tggatcgttt cgatcaaagc ttttcatgat
    11341 tcgtacgcga atatcctcaa atttacgaat gagatgtttg acaatgtgaa aacgatccac
    11401 aacgagtttt gcctttggaa aacattcctt tatcagagag gcataagaaa cattcatgtc
    11461 cgtcacgatg acctttacct tcaatcgtgc gcgtcgttca aattgcataa aataagaaac
    11521 gagagccttt ttcttccgtg aaggaagaat atccagtaat tttccagtat cgccatccat
    11581 acaagaaaag gagtagggac cgacttgatt tttcagtgaa cgaaattcat ccattaataa
    11641 acaacgcggt aaccaatttc tttttacttt tgtcatgggg atcagagaag cgagtgtccg
    11701 agaaacggtc gaccaagaaa cgccgcaatg cttagcaatt gtcgtcattg cttgtttttc
    11761 agttagctct tctaaaattt tatagcgcac ttgtttagcg ataaagcagt ttctatcgac
    11821 aatgtaggta ttcgccgtaa aataggtgtg gcagccacga cagtggtagc gttgtttcgc
    11881 taaagcaaga taagtcagtt ggttcgcaca tttattaagt tgaatgagcg attttttctt
    11941 tccgttctta acgatcatgc cttcatgatt catctgttta cacaaaggac agcgacgaat
    12001 ttctttactt aacgtcgcag aaacaacaag actcttgata ttcttaatgc tcttcgattc
    12061 cacatttgtg atgaaaatgt ttttatcttt gattccaagt agtagtttga tatgatgaag
    12121 atcaggcata cagaacgctc ctttgattgt gttgtggtga ttcaattcta acggagcttc
    12181 tgtatgtttt gttttttcat gctcaaaagg gaccgataga atgattttct atc
//
LOCUS       DASNNU010000075        12161 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.075, whole genome shotgun sequence.
ACCESSION   DASNNU010000075 DASNNU010000000
VERSION     DASNNU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.075"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            140..460
                     /locus_tag="U8116_002068"
     CDS             140..460
                     /locus_tag="U8116_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435050.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="U8116_002069"
     CDS             912..1112
                     /locus_tag="U8116_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN8435051.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="U8116_002070"
     CDS             1259..4048
                     /locus_tag="U8116_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN8435052.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="U8116_002071"
     CDS             4095..4622
                     /locus_tag="U8116_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEN8435053.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="U8116_002072"
     CDS             4643..5833
                     /locus_tag="U8116_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN8435054.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="U8116_002073"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="U8116_002073"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEN8435055.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="U8116_002074"
     CDS             7966..8640
                     /locus_tag="U8116_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN8435056.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="U8116_002075"
     CDS             8637..8978
                     /locus_tag="U8116_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435057.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="U8116_002076"
     CDS             9008..9367
                     /locus_tag="U8116_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEN8435058.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9891..11975
                     /gene="cadA"
                     /locus_tag="U8116_002077"
     CDS             9891..11975
                     /gene="cadA"
                     /locus_tag="U8116_002077"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN8435059.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
BASE COUNT         4070 a         2115 c         2610 g         3366 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa
    12121 tgaaataaaa gaaaataact tatttttttg atggaaaaaa t
//
LOCUS       DASNNU010000076        12104 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.076, whole genome shotgun sequence.
ACCESSION   DASNNU010000076 DASNNU010000000
VERSION     DASNNU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.076"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..921
                     /locus_tag="U8116_002078"
     CDS             <1..921
                     /locus_tag="U8116_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435060.1"
                     /translation="QQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFL
                     GLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSS
                     LFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDA
                     VAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKN
                     TVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRY
                     LSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1066..1806)
                     /gene="truA"
                     /locus_tag="U8116_002079"
     CDS             complement(1066..1806)
                     /gene="truA"
                     /locus_tag="U8116_002079"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN8435061.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1997..2632)
                     /locus_tag="U8116_002080"
     CDS             complement(1997..2632)
                     /locus_tag="U8116_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEN8435062.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2779..4020)
                     /gene="sstT"
                     /locus_tag="U8116_002081"
     CDS             complement(2779..4020)
                     /gene="sstT"
                     /locus_tag="U8116_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEN8435063.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4218..5216
                     /locus_tag="U8116_002082"
     CDS             4218..5216
                     /locus_tag="U8116_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435064.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5229..7247
                     /locus_tag="U8116_002083"
     CDS             5229..7247
                     /locus_tag="U8116_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEN8435065.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7307..8023)
                     /locus_tag="U8116_002084"
     CDS             complement(7307..8023)
                     /locus_tag="U8116_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEN8435066.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8250..8792)
                     /locus_tag="U8116_002085"
     CDS             complement(8250..8792)
                     /locus_tag="U8116_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN8435067.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(8797..9024)
                     /locus_tag="U8116_002086"
     CDS             complement(8797..9024)
                     /locus_tag="U8116_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN8435068.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9103..10983)
                     /locus_tag="U8116_002087"
     CDS             complement(9103..10983)
                     /locus_tag="U8116_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN8435069.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11172..11786)
                     /locus_tag="U8116_002088"
     CDS             complement(11172..11786)
                     /locus_tag="U8116_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8435070.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11928..>12104
                     /locus_tag="U8116_002089"
     CDS             11928..>12104
                     /locus_tag="U8116_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN8435071.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT         3791 a         2393 c         1931 g         3989 t
ORIGIN      
        1 caacaatcta gagcacaaga attgaaagtc ccactgggaa aagaattaag tggtatggct
       61 cctagtgata tgctgacaca tattttaaat aataactata acaactatac tattggatta
      121 ttcttatttc tcggattgat tggtgctttt atttttataa aaaaattaac tgctgatgat
      181 ttatttattt tctttcttgg tgtatttgtt cttttttgtt caaccaattt atttccttgg
      241 caacttttta accatacacc agttaagagt ctacaatttg tctggcggtt aaatggtttt
      301 tcttctttat ttatagccta tacgatgagt atagtgatct attatatttt ttctactaaa
      361 aataattcat ggaaaataat ggtctttacg tttcttgcac taatattgca tctctcagga
      421 gtaagcaatt ctatccatgc taacaaagat tcattaacac ttatagcacc tgaagacgct
      481 gttgctatag ctgaaaatta caatcatact gattatgcaa acaaagagtc aatctatcat
      541 cctgatatga ttaataatga tcaatattta ctaggtaacc aggaaataaa tccaacaact
      601 cattttatga gtaacaaact tacaattgaa ttagataatt cgaataataa aaatactgtc
      661 ttgacgacac ctatttatcg ttataaaggg caagtggctt ctattaatgg caaactagta
      721 caaacaaaat tatctaagtt tggcacaact gaattaacta ttcctccagg tataaataaa
      781 gtcgttatta cttatcagta cacaaaacta gcaattgctt caagatattt atctatcgtt
      841 actttgattt tatttttact ttatcgattt actttttcta aacaaaaaca accgagaaga
      901 gaagtacaac attctcacta gatttttatt taagaaagac tttttagctg tacacataaa
      961 caggctaaaa agtctttttt tatatttctt taatttgctt catacattat ttaacatcga
     1021 tatttatact gtgaaacatg cgtttcatcc attatcaaag tatttttatc gataataaac
     1081 ttcatccaaa aacagacctt gtgcagggac ggtttctcct gcatgttgcc gcactttgct
     1141 ttcaaatatt tcatcgattg tttctatcgg catggcacct gtgccgattt ctagtaatgt
     1201 ccccatgata atccgcacca ttttatataa gaatccgtct ccgacaaaag taaaatgaag
     1261 catttctccc tttcgttcga tagataattc atcaatcgta cgcactgtgg atttttttga
     1321 ttttttcaat gcagaaaaac caatgaaatc atgtgtaccc actaattttt cgcaagctgc
     1381 attcagcttg tccatatcca actcttgagg gaaataaaag ctgtggtttc gctcaaatgc
     1441 tgaaggaatc acgttgttcc aaacataata gctgtatttt ttgcccaccg cattatatct
     1501 tgaatggaag cgctcaggca tttcttcgat tcttttgatg acaatatccc gcggaaggta
     1561 acgattcata aagtcgatca tttctgccag tccaatttct gtgtttgttt taaaatttgc
     1621 tacttgtcct cgcgcatggg tgccagcatc tgttcttcct gatccaataa tttctatctc
     1681 ttcatttgtc atctgatgca atactttttc gatttttcct tgaatggttt tatcgtcatc
     1741 tccaagtctt tgccatcctg aatatctttt tccgtcatat tcgattgtca acttgatgtt
     1801 tctcatattt tagtggctcc tctgtcttcc atttggtctt ctctctacct ttttaagatt
     1861 gtagcacaaa atatagctcc ttgcttctct aagacagaag ctatacaaga aaactgtgga
     1921 aatatatatt tcagaaaaac aaaaaacagc caccggatga agtatccgct gactgttcaa
     1981 tttgacggct caagttttac tgtttgagat gcaagatact caaccaggca atgggcacat
     2041 aattaaagaa aaaactgatt ctttggcaaa aacctatttt attcagtaat ggccaagaat
     2101 ttatagaagg aattcttgcc agtccataaa tcgccgcaaa aagcaaactg atcacagaca
     2161 gtccaaggta taagtggctt acgtccattt gcccattttt tcgatagatc aagaaaagaa
     2221 gaactggaaa caataaaaaa ccactgtaac ctaaacctga tccgatatta tggacccatg
     2281 tcgagacatt ccacgaagat tcatgggtat tgatactgaa caaacctgta aagatacaat
     2341 cacctatacc atagaacgcg atggctgctg taactagggt agctaacaca cgagatgtac
     2401 taacaaatgc agcatacaat gcaggcaatg acaggatgaa aaagatccct gaaaccacag
     2461 accaaataag aaacgctttt cttacaggac tggaaacctc tccaaataca ctgatcacgc
     2521 tcttcatttg atctaattca ggataataca atcctaatac gtacggagtc aaaaaatcac
     2581 tcagcacgcc taataataaa aagaaaaaac caaatttacg taaaaaatcc atgatacgcc
     2641 tccaataaca attacctatg cttttaattg ttattataaa cgactttatc ttgatttaac
     2701 agaaacctct atttacacga acatctatat aactcattaa aaactcgaat cattttaaaa
     2761 atactaaata tctaacgttc aacgttcact taatccatct aaacaatctt tcaatttgat
     2821 tttttcccca cttcttttac gcgcgcctag ctctcctatt gcagtaaaca acagatcact
     2881 tgacgaattt aaggcagttt ccattgaatc ctggataaca ccgataataa atccaatacc
     2941 gacgatttgc atcgcgacat cattagagat accaaacaaa ctacatgcta atggaatcaa
     3001 taacagtgaa ccgcccgcaa cacctgaagc tccacaagca gctaaagcag acaaaataca
     3061 taaaaggact ttcaaaacaa aaggtgcttc aatccctaaa gaatgtacag cagtcaacgt
     3121 catcaaggaa atggtaatcg cggcaccgcc catattgatc gtagcaccta acgggatcga
     3181 tatggaataa gactcttttg tcaatttcaa tctttcagcc aacatcatgt ttatcgggat
     3241 atttgcggca gaacttcggg taaagaaagc gggaagtgcg ctttctttca aacaaaagaa
     3301 aatcaatgga tagggatttt ctttaataaa caaaaacgtg aggaatgggt acacaactaa
     3361 tgcgacaaac agcatcgtac cgatcaaaac aagtaaaagc tgcagatact ctgccaatcc
     3421 agcgattcca gtcgtagcaa ttgattgata aaccaatcct aaaatcccta tcggagcaaa
     3481 tgcaataatc acttgtacga cttgtgacaa cgctatagat atctgatcca ccaactcttt
     3541 tgtcgattct gaacgtgctc gaaaagctag tcccaacaat acagcccaga ataagacgcc
     3601 aaggtaattc gcatcaataa gagattgaac aggattttgt gtgacattcg tcaacatagt
     3661 agataaaaca ctttccaaac tttgcggtgc tttttcagat actgtttctt gtaaagtaat
     3721 aggaacagta aaaattgaac ttacaataac tgctacaagt gcagaaagca atgttcccac
     3781 taaatataag atcaaaatag gcttcacgaa tgtcttattc ccaattttat gcttggcaat
     3841 tgacgccatc gtcaagaaaa aaaccagaat aggtgcgatt gccttcaagc aactgacaaa
     3901 caacgttccc aatattgaaa taaatgtcca tgtggggaaa aagataccaa acaatattcc
     3961 gactaaaata ccaacaacaa tacgttgaac taacgacata tccaaaaact tctctaacaa
     4021 atgaccaccc tttcacaatt ttaacaatca ttgtatcatt ttatgcaaag aaactaaagg
     4081 tataattttc taaaaattct aacaatacaa acaaccattt cttgctaatt ttgtactttt
     4141 tctgtctaca cgttgttttt tgagatattc taagttagga tgttattaat gggcaactta
     4201 tcaggaaacg ttttcttatg tttttgtcca atttttttgt gaggtgttta ttcttgaaaa
     4261 aaataaagaa ggagagttat ggcttcctga caatctttgt cgcttgtata atgatggttt
     4321 tacagcgtat attgatttca gcagtcacta ttcctacata tgcaacaaac cctggtcgat
     4381 tctacatcta tacattttta caaacttccg ttgttattgg tgcaaatttg atcccacttt
     4441 acatgggtta ccaatatcaa aaaatcgccc gtttaaaagt tttacattat ttaagtcgtt
     4501 ttgcctttat attcctaata gccacactag tttgcaatat ctttttttac gtacaagctg
     4561 gcagcttgaa tattcgtgat tattggctta tcctaacacc tatcagtcag aattacttta
     4621 cttatgctgt ttcttgtact ttgctttttt gtagtattcc ttatacagtt ggatttttaa
     4681 ataaattatc gaaaacaaca attaaatatc tactattatt tttaacaatt ggattagttg
     4741 gctgctctac tctatttaat aaagacccgt ttgcattaca gaatggacag agtattttat
     4801 ggatttttta tttattttta atgggttatg gtttgaaaga atggaactgg aaagaaaagg
     4861 tccgctataa gcttcctcaa gtatttatca gcttgattgt tttattcggt ttgattattt
     4921 tgatgacaca aatttcactc atcatcagag gaaatacaga tacagcgctg aggttttcta
     4981 aaccatattc tctcttcgca atgtattata ctttctcttc gtttttattt ttagaaatgc
     5041 tatctgataa attaggtata aaaggaagag gaaattttct tgctgtattt ttgattgtca
     5101 ctcaagttat atgtaatttc cctttagttt cttatcgcgt atctacattt atgaaacgtg
     5161 aatttccaaa ctcagggaag gcatggctga taaatatcgg gcagtttttt atttgatatt
     5221 tattagcagt gtccctccta gttatctttt tattttatat acaaaaaatc cgtttcattc
     5281 atcgtttcat cgaatacttc gcttttgatt cgattgaaca attaactcag cgttggaaaa
     5341 aaagaattca ttggcttttt gttcataaaa gttatttttt agttgcagca tttttttact
     5401 gctttacctt cgtgcagatt ttcgttttag aaccaaaaga aggttggaaa gaaactatcc
     5461 aggttgcctt aaaaatattt actcagcgac aagcacccat gatcctgaca atcatcatta
     5521 ttatgggatt tttcttctta ctattgttga tttccaatcg tttttggtac gccttgactg
     5581 ctaccttgat cattaactta ctgctcacta tttcgacagt aatcaaaatg gaaatgcggg
     5641 aagagcctgt ctttccttca gatttaaaaa tgctgaccgg attgtcagaa ctattatcga
     5701 tggttagccc agtcttatta atcgtgggcg gattgatcct tctcctatta ttgattacta
     5761 gtattattgt acaacgtaga ctacaaaaac agtattcctt gaagattcat tggaaacgtc
     5821 gatttatcag tatcgctgta ttattaggat gtttttcggg tgtctttttc attaatcaca
     5881 aaaattcgcc ttctttcttg ctgtttaatt tatttaaagt aaacaagact ttctttaacc
     5941 agcaaggcgc cgtaaaagat aacggaccag tcattcaatt cctaaacaat atcgacatta
     6001 aaatcatgga agaacctgct ggctattcga aagaaaaaat taatggaata atgaaaaagt
     6061 acaacacaaa ggcagatgaa atcaaccaaa caagaagtga ctggttaaac aatcagacga
     6121 tcgttctcaa tttaagtgaa agcttcagtg atcctgcacg tgtccctaat ctgacagtac
     6181 ctacagaccc tattccttac atcaaaagca ttataacaga aggaacagga ggattgatgt
     6241 tatccgtcgg ttatggtggc ggaacagcta acatggaatg ggaaggactg acaggcttga
     6301 gtatcagtaa cctgtctgct tcgttagtta caccttatac ccagctagtg gagcgtcaga
     6361 aaataagtcc aaatattacc gatctattcg atgaaaaaat agccatccat ccttttacag
     6421 ctgctcttta tcgcagaaaa caagtgtttg ataaatttgg ttttgacagg ttctattatt
     6481 tagacagtcc ggataaatta acttatacgg ataaaataga acaaagccca cgcatatcgg
     6541 atgattcagc atacaaagaa accttgaaag ctttacaaag caatacggaa acgtcacaat
     6601 tcatccaatt atccactatg caaaaccata tgccttatga tgattactat agtgaattga
     6661 actacactgc tgaaggaact gctgtcattc ctaatagaag aaaagaatta caaacttata
     6721 tgcaaggcct tcactatacc gatacagcag taaaaaaatt catccaagaa atcgacaaaa
     6781 tcgataaacc gattaccttt gttttttatg gtgaccattt acccgcaatc tatagtggga
     6841 atgattcgaa aaaatatgga ttagaacagc accagacaga ttatttcatt tataacaaca
     6901 agtatagtcg tgaacaagca gctacgttaa ataaagaagt tgttgctcct aataactttg
     6961 gtgctatggc tcttgagcat gcgaatatca aagtaactcc ttactatgcg ttgttgacag
     7021 aagtatctaa tcatctacct gcattaacaa ttgatcctac agaaagcttg tctaatcgtt
     7081 ttaatggaaa acaggtcttt gttacagagg atagtcaaat tatccaagaa aaaaatttaa
     7141 ctgagaaaca aaaagaaatt ctagaagatt atcgtctgat acaatatgat cttgttgcag
     7201 gtaaccagta cagtgcaaca tgggcgacac aaaaaataaa agactaacaa ataaactgag
     7261 caaggggttg tgataaaaga catgtcacaa ccccttgttt atctcattaa tttgcttgtc
     7321 cataaacgat ttgattaaag ccatttttca attcattatt cgctgtctcg ttgaagtaaa
     7381 gataattatc gggattcccc tgattttgtt gtgtcaccaa tacttttggc tgattttgct
     7441 gaaacccgaa tagatagacg tgctttttca gatatggttc tgtctctgca tcggaataga
     7501 cggctgtgat ttgatagtct gcttgacctg tcccactttc tgaccattct gcagaaacag
     7561 gcactccacc aatagccatc ttccactccc cgttcaaaat agcttgaggg actttaagac
     7621 catataagtc cacactcatt tgattcgtat aacttttgta ttgttgtccc attgtttggc
     7681 cccatttggt cataaatgcc tctaattgtg cggatttgtc tgcattccac agtgtttgtt
     7741 caacttgttc gctgctttct gaagaggaag atgaagcctc tacagaagaa cttgaagtag
     7801 atgtttgagt tgtttcagtc gttttggaag actctgacga agtagaggta tctgctgttt
     7861 ctgtacttgc tgcagttgac ttgctctctg tgctagttgt tgtagtagtt gcaactgttg
     7921 ctttcgctgt ttcttttgtt gattcttttt tcgattcatt cccgttggta caaccagcta
     7981 acaaaaaagc tagaccaatt gttactaaaa tccttttttt caattaaaaa actcctttcg
     8041 attactccca cttctttatt ctatcaaata agcaatgtga ttttattgat ttttcgtatt
     8101 ttctcatcaa tctttcaaat cttctaaaaa ctgagaagca cccgctgatg ttgtctgtaa
     8161 tacatcagcg ggtgcttctt ttattttaat cttcgtgatt ttccaattta cctcacgctt
     8221 tatatttgta tttgaagaaa catagacttt tagtcatcat cgtcttcctc atccaaatct
     8281 tgtcggacag tttttccttg gagtctttgt acttggtaga tgatttcttt attgaaactt
     8341 aaaagttcaa caagcaattg ttgcaagcca tatttttctt ctttttcagc taatttgatt
     8401 gcccgtgtga taaagacatt ttggaaatca aaatcttttg caaagttttc caacattgtt
     8461 gaggctggta aatatttgtt ttgaccatgt tcgtaaattt ttccataacg catgacttct
     8521 tctaaagtag acggaacgat ttcattttca atcactaact catgagagat tttattgaac
     8581 catctcgctt cttgatcagc atattctgaa aaaagggtac ttaattcttg cgaagatgta
     8641 cccaaaacat acaattgtgc ttggatcaat ttcatttctt ctaatttaaa atttgctaat
     8701 acatgcccca ccatggcgcc agcagttggt ttatgatggt cgatatctgc ttgttttgtt
     8761 tcttgaacaa atttttcttc cactatattc attgatttac gcctctttta cttttgctga
     8821 ttggacttca taacctaatt tttccacaac tgcagctaat tgttcagggt ggacttcatc
     8881 tggatcaaag tttgctttga ttttgctcgc attaaacaaa actttcgtat tgcttacccc
     8941 aggctgttga gaaaccgcat gttcgatttt ttgcatacag cttggacaag ctagtgtatc
     9001 taattgcata ataatttttt tcatcgtagt tcttccttcc tttttcttga tagtcttatt
     9061 ctagattaca gcttccataa aaaaattgat tcttatcaag ttttacgatt tttcttcttt
     9121 gctttcgtgt ccaaaataca ataaacgcat cgcgttaaag ataacgatca agatactagc
     9181 ttcgtgaacg aacatcccac tagccatgta aataaacccg gcaaataagc cgttgagtaa
     9241 aaataagacg acaccaatgg caatcactac attttcacgg gtatttaaaa cagttttctt
     9301 agccaagcgg taggcatgaa ccaacgattc aaaattagat tgcatcaaga caacgtcact
     9361 cgtttcgata gcaacatccg ttcctgatcc catagcaatc ccgatatcag ccgcagccaa
     9421 agagggagaa tcattgattc catctccaat aaaagcaacg cgcaatcctt gttcttggta
     9481 ttttttcaca aattgaacct tttcgtctgg taataattca gcatggactt catcaatgcc
     9541 taacatatca gctacataat cagcagtcat ctgattatca cctgtcagca tcactaactg
     9601 ttttgctcct gcttgtttca acttagctaa ttgttcagcg acttctggtc gaatcacatc
     9661 tgacacacct atgatttgag tgattttttt gtcgattgct acaataatga ccgagctgcc
     9721 tgtttgttgt aattgatgta aatcttgtac ttgctttggt gtgagagaaa gtttgtgagc
     9781 ggcaatcaat ttggtgttgc ccgcacaaac tgtatgacca tcaatctcag ctgtgattcc
     9841 ttgtcccttg attgtctgat tatctttgac tgctaattgt tgaaaatcaa tgtctttctc
     9901 ctgagcataa tggatcacgg cgcgccccag aggatgatcg gataacgttt ctaatcgagc
     9961 cactaaagct aagctgtccc cttcatcctc tgtatagttc ttgatagtgg atacagcagt
    10021 agttcctttg gtcaatgtac cagttttatc aaagacaaaa gtatcgattt tggaaaaagt
    10081 atccattgct tcaccgcctt tgactaagat gccccgtttc gctccattac cgattcctgc
    10141 tacattcgaa acaggcgcac caatgactaa agcgccagga caaccaagaa ccaaaacagt
    10201 gattgctaaa cgaaaatcac gactaatcag accaacaacc aacgcaataa gtaaaacggt
    10261 tggagtataa tactgagaaa atcgatcaat gaatttttcc gcatgggatt tggtatcttg
    10321 tgcttcttct actaattcga taattttggc aaaggtcgta tcatctccga ccttagttgc
    10381 ttcaatcgtc acgtagccat ctgctaaaat cgttccagag aagacttcat ctccactgat
    10441 tttgacgact tgtcgtgatt caccagtcac tgcggcttca tccgcatacc ctttcccgtc
    10501 aatgactcgt ccgtccacag gaatagctcc accagttttt actaagacga tatcgccttc
    10561 ttccacgtca tcgatttcca cttcttcagt cgttccatct tcttggacaa cactagcagt
    10621 agatggggcc atctcggtca aagccttgat tgactcccgc gttttggtca atgttttttg
    10681 ttccagatag ctaccaaaca aaaacaagaa tgtaacgata gctgactcct tgtattctcc
    10741 gatcataaag gcaccaagca cagcaatagt aacgagtaaa tcaattgaga taactttgac
    10801 tttcactgcc tgatacgcat ggatcataat aggtatgacc gcaataatag aagctatggc
    10861 tagagcaatg ttatagaaca agacaaaacc agtaaatttt ccgataaaac caaaaataat
    10921 caacacagct gataaggcgg tgatatgatt tcgatgctct tggatccact tttgtaattt
    10981 catctttctc cctcttttcc tttcattctt acgagactaa gtatagatac ttatccatca
    11041 taaaaaattg accactatca agtttagtca aaaaggcggt tttcttttgg tagaatacac
    11101 tacaaaaaat agttagcaag aagactcact aaaagtgatc ttgccaacta ttgtcgtttc
    11161 tatcgaaatt ttcattgaat gattttcctg tacagcatac atcggagatc tatttcttta
    11221 tctaagattt ttatcttgct aattttttcc tttagtcttt caaatccacg tcgttcatag
    11281 aattgatacg tacagccact atctgtaaaa aggaatattt cttttccttt ctctcttttt
    11341 tcaaattctg ttagcaactt ggtaccgatg ccttttgttt tcaaatcagg atttgcagct
    11401 aaaaaaatga tctctccatc tggcgaatgc ttctttaaat atgctgcaag catttgttca
    11461 tttgctgtct cgtaagctcc tttactttct ttagcaaata tattttgtaa aaaatcgaat
    11521 atcttaacat atatttgctg tccaaaagaa cgatgtttct ttttttctcc ttttatctcc
    11581 gccagcaaaa cacctgccaa ctcgtcctct gcatacaaag caagaatttg agttgctcga
    11641 ttcaactcca tatacaaaaa atatcttccg taaagattca gcagaagttc attttccata
    11701 tatctctcaa agttcattcc ttcaacagca aaatagatag cttttttgta atctttcttt
    11761 tctaagggtt taaaagttag tttcatcatt tcgcctcctc aacttcaatg attgtgttat
    11821 acttacatta taatagacac agtgtctact atctataatc aatcatctat aagtttgtcg
    11881 attaagcgac agctttgctt tgatatgtcg gataaggaga taggtaaatg aaaaagacag
    11941 atagacgcgt caaaaaaaca gaaaaagctt tggctgaaac actatccaca ttattagtaa
    12001 ataaaaagat tcaagcaatc actatacgcg aactaacaga aacggcagat gtacatcgtt
    12061 cgacttttta tacacattat aaggatatct atgatctata tgat
//
LOCUS       DASNNU010000077        12029 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.077, whole genome shotgun sequence.
ACCESSION   DASNNU010000077 DASNNU010000000
VERSION     DASNNU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.077"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..711
                     /locus_tag="U8116_002090"
     CDS             <1..711
                     /locus_tag="U8116_002090"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN8435072.1"
                     /translation="GCSDGGLGLLQSLGASLEGYTEGNPLLSTKKLHFDRRNVLVSKV
                     KLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFWEVDLDDI
                     PGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVIITGEGRIDQQTIHGK
                     VPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLE
                     KTKILGQTLSRFFTILPK"
     gene            969..2471
                     /gene="eat(A)"
                     /locus_tag="U8116_002091"
     CDS             969..2471
                     /gene="eat(A)"
                     /locus_tag="U8116_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN8435073.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2499..3065
                     /locus_tag="U8116_002092"
     CDS             2499..3065
                     /locus_tag="U8116_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN8435074.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            3276..4337
                     /locus_tag="U8116_002093"
     CDS             3276..4337
                     /locus_tag="U8116_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN8435075.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4340..5236
                     /gene="murQ"
                     /locus_tag="U8116_002094"
     CDS             4340..5236
                     /gene="murQ"
                     /locus_tag="U8116_002094"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEN8435076.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5250..7232
                     /locus_tag="U8116_002095"
     CDS             5250..7232
                     /locus_tag="U8116_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN8435077.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(7297..7743)
                     /gene="nrdI"
                     /locus_tag="U8116_002096"
     CDS             complement(7297..7743)
                     /gene="nrdI"
                     /locus_tag="U8116_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEN8435078.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            7954..9378
                     /locus_tag="U8116_002097"
     CDS             7954..9378
                     /locus_tag="U8116_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN8435079.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(9559..10332)
                     /locus_tag="U8116_002098"
     CDS             complement(9559..10332)
                     /locus_tag="U8116_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN8435080.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(10329..11234)
                     /locus_tag="U8116_002099"
     CDS             complement(10329..11234)
                     /locus_tag="U8116_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN8435081.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(11388..11708)
                     /locus_tag="U8116_002100"
     CDS             complement(11388..11708)
                     /locus_tag="U8116_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEN8435082.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         3867 a         2132 c         2584 g         3446 t
ORIGIN      
        1 gggtgtagcg atggtggtct aggattactc caatccttgg gtgcttcttt agaagggtac
       61 acagaaggaa atcctttatt atctactaaa aaacttcatt ttgatcgaag aaacgttctt
      121 gtttctaaag taaaattgat tatcgctgca gatgttacca atccatatac aggtcaacaa
      181 ggagctgctt ggcaatttgg aagacaaaaa ggaggaatag atacgacgct agctttctta
      241 gataaacaag caggaaagat tgctcagcaa ataaaagatt tctgggaggt ggatttagat
      301 gatattccag gaagtggagc agcaggcggt attggtggag cccttttttt gttgggagcg
      361 aagatggttt ctggtttcga tttagtcagc caaatcactg gattggaaaa aaagatcgaa
      421 caagcagatg tcatcattac aggagaaggg cggatcgatc agcaaacgat ccacggaaaa
      481 gttccatacg gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat cgctatttgc
      541 ggaagtagag accaagagct agaaggactt tctgattttc tcccaattgt cttaagtatt
      601 catttgggtc caataggttt agaagaagca atggaacaca ggcaaacatt agaaaaaaca
      661 aaaatcttag gacagacact tagcagattt ttcaccatcc tcccgaagta gttgacaaaa
      721 tcattttctt tatgctatcg taatttcaaa tataggacaa agtgagggat ccgtgcattg
      781 aaaaattaag tcgattgatg aaagctgcat tcagactgta ccttgtggag acaaggaagc
      841 agtgtgcaga ttttcgggat tggcttggtt ttttcgatgt atgggtcttt ttgtgtgtat
      901 ccaacccttg catcctcttt agccagttct gaaaatagga ctggtctttt tttacgaggt
      961 gatggaaaat gtctaaaatc gaaataaaaa atctgacatt cggctacgac agccaaggca
     1021 cattattatt tgaacaagca aatctaaatt ttgacacaca atggaaacta ggacttatcg
     1081 gacgaaacgg tcgaggaaag acaactttac tgaatattct acaaaacaaa ctaccttatc
     1141 aagggcaagt aatccatcag caagaatttg cctatttccc gcaacagaca aaagataaag
     1201 aacgtttaac ctattacgtg ttaaatgata ttacggattt tgagatatgg gaaatcgaaa
     1261 gagagctcca attgatgcaa acagatcctg aaatcttatg gagagaattc agcacactat
     1321 cggggggaga gaagacaaaa gtcctactgg cacttttatt tgtggatgac actcatttcc
     1381 cgttaatcga tgaaccaacg aatcatttgg atatctctgg tagaaaacaa gtagcggctt
     1441 atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga tttatcgatg
     1501 aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact ggaactttat caagggaatt
     1561 tctctatcta tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct caaaatgaaa
     1621 aattgaaaaa agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc gaatggtctc
     1681 gttcccgaga aggagataaa acaaagaaac aagtcggatt catcgatact gaatctagac
     1741 gagtgaataa aggagcagtg ggtgctgatg ctgcacggac gatgaaacga tccaaagcaa
     1801 tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa actattaaaa gatatcgaat
     1861 atatcgattc tttgacgatg aatagccaag cgtctcacca taagcgactt ttaagcgtag
     1921 aagatcttca attagggtat gaaaatctgt tattcgagcc aattcatttt acaatcgagc
     1981 ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc attatccatt
     2041 atcttctggg ggcattcaac ggcaaggtta taggagaaaa ataccagcca aaacatctga
     2101 gcattagtta tgcaagccaa aattatgaag acaatcgagg aacgttggcg gaatttgcag
     2161 agaaaaacca agtagactac caagcatttt tgaacaacct ccgaaagctt gggatggaaa
     2221 gagatgtttt tcataacaag atcgagcaga tgagtatggg ccaacggaaa aaagtggaat
     2281 tggctaaatc tttatcacag ccagctgaac tatatacatg ggatgaacca ttgaattatt
     2341 tggatgtctt caatcaagaa caattagaac aactgatctt gaacgtgaaa cctgccatgt
     2401 tactagtgga acatgatcaa accttcctgg ataaagtatc tactgagatt atttctcttg
     2461 agagaatcta acccttgtaa tctcaagaac aacaaggtat ggttaagggg aactttgcca
     2521 acgaaggaga gaatcacatg ctttatgtaa cgagacacgg agaaacaact tggaacgcac
     2581 aaggattagt ttgcggacgg gcagatgtgc cgctgactga aaaaggacag atgcaggcac
     2641 agaaactagc agaaaaggta gtagatttgc ctgttccaat cacgaaaatc atccattccc
     2701 ctcttcaacg agcaagagat acagcacaag cagttgctga tcgtctcagc ttgccgctga
     2761 ccgttgatga acgattggta gaaatggatt ttggggatta tgatggactg ccaagcaaag
     2821 atgagaactt tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat ggggagtcag
     2881 tcttagatgt gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa gacgaagaaa
     2941 atgtctatct gttagtttgc cataatgcct tgataagagt gatcaatgct tattttcatc
     3001 cgatgcctaa tgaggggttc tttacattca tggtagataa tacagaattg gtttcttatg
     3061 agtaattatg tttttgtgtt atttaaataa aatatttcaa ggcactgctt ttgcttagca
     3121 gtgccttttt taggttatga caattctttt gataatataa actcaacctt tagaaatgaa
     3181 atttaattat tttaataaaa gtattgaaat tttgtttctt tagatatata ctctagtcat
     3241 agaaatgaag acggatacag aaaaagaggg ataatatgtt tggcttttca atatttatga
     3301 acgattcttt gtcagaagag aagaaacagt atatccgcca aatggcatcc aatggctttg
     3361 tcggtatttt cacttccatg catataccag aagatgatgt ttcggcatat caaaagcgat
     3421 tgatcgaact aggaagcgaa gcgaaaaaga ataaactaga attgatggtc gatatttcag
     3481 gagaggcatt ggaacgagcg ggtttttcat tagaagatct aacgcctttg aaagcaattg
     3541 gtgtaacagg tttaagaatg gattatcata tttccaatca gcagattgcc gactggtcac
     3601 atcaattgaa aatcagtttg aatgctagca cgatcacgcc taaagatatc gatgaattga
     3661 aagaagcaga agctgatttc tcacagattg aagcttggca taattattat ccacgtcccg
     3721 aaacagggct ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa attcaaggtc
     3781 tccttgtcca aggttttgta ccaggagata cagagttgag aggcccacta tatcaaggat
     3841 tgccaacttt ggaagaacat cgagggatcc atcctcttgc aggcgctcta gatctattag
     3901 caagtaacac ggatattgtt tacatcggag atgcaggtct gtcagaaaac gttcaggagc
     3961 aatttgcttc tttccaaaaa gaacagacag tcttgctcca tacggaacct gtagatgaag
     4021 aattttacga atatatatta gggaagcata cgaatcggca agacgatgca agagatgtca
     4081 tacgaagcgt agatgcacgt tttcgagaga ttccgcctat cccagcaaga aatacagcta
     4141 cgcgtatgaa agggtctatt actttagata acgaaaagta tttgcgctat atgggtgaga
     4201 tccaattaac caaatatgat ttgccagcgg atgaaaaagt gaatgtagta gctaaagtta
     4261 tcaaagaaga gctaccattg atcaaccaaa tcaaagctgg tatgaactat caatttataa
     4321 gaaaagaagg aagataaaaa tgagcaaaat caatttagag aacctaacaa cggaacgcag
     4381 aaaccaagaa acttttggat tagatgagat gagtatttct caagccgttc aactaatgaa
     4441 caaagaagat cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta tcgaaccagt
     4501 gattgctaac acgattgagt cctttaaaaa aggtggacgt ctgatttata tgggagccgg
     4561 aacaagtgga agactaggtg tgttagatgc tgctgaatgc gtaccgacat tcggtgtaga
     4621 accggaaatg gtcataggtc taattgctgg tggagaagaa gcaatgactg tagctgtcga
     4681 aggggccgaa gattcttatg agctagggca agaagatcta atcaagctga atttgacaaa
     4741 aaatgatata gttattggta tcgcagctag cggtcgtaca ccatatgtga tcggtggatt
     4801 agattacgca aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata aacaagccga
     4861 aatcagtaaa cacgcggatt tcccaatcga agtcgatgca ggtccagaat ttttgacagg
     4921 ctctactcga ttgaagtcag gaacagcaca aaaacttatc ttgaatatga tttctactat
     4981 cagtatgatt ggtataggta aagtattcaa taacttgatg gtcgatgtca aaccaacaaa
     5041 tgaaaaacta atcgaacgct caaaacgaat catcatgcaa gcgacagaag ctgattacga
     5101 aacagctgaa aagtactttg aagaagcaga tcaaaatgtt aagctggcta tcgtcatgat
     5161 tttgacaaat agtgataaag aggaagctca agcaaaatta gctgaagcaa acggatttgt
     5221 aaaagggacc ttatgagggg gaaaagacca tggcaaaaaa cgaactgact ttggaagaac
     5281 tggcaaaaca aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc attgtccact
     5341 gcatgactcg tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc tctgaattaa
     5401 aagcgattcc aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc atcatcggac
     5461 ctgggaaagt gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg aaattaggag
     5521 aaaaactttc ttctgtaagt acagctacag caaattcatc agcatctgga aaagaccgtg
     5581 taaacgaacg ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa ccatctaaaa
     5641 tcaaatcggt attaaaagac atttctaata tctttgtgcc aatgattcct gcatttgtcg
     5701 gtactgggat cgtggcaggg attgccgcag ttttagcaaa cttagtcaca gccggaacac
     5761 ttgatgcagc aacatggcaa caatatatcg atgtgatgaa catcttgaaa aacgggatgt
     5821 ttagctacct agttatctat acaggtatca atgctgcaca agtttttggt gcaacaccaa
     5881 ctcttggtgg agttatcggg gccgttgtga tgctgacagg gatgaatccg gaagcaccac
     5941 tgaaaaactt atttactggt gaagctcttg cagctggaca aggtgggatc ctaggtgtca
     6001 tcttcgctgt ttggctattg tcaatcgttg agaaaaaact gcataaagta gtacctgatg
     6061 cggtagatat catcgttaca ccaactcttt cattgattgc catcggtctg attgaaattt
     6121 tcttcatcat gcctttagca ggtttcattt ctgatggcat ggtcggtggg atcaactggg
     6181 tacttggcgt tggtggagca tttgccggct tcgttctagg cacattattc ttgccgatgg
     6241 ttatgttcgg actgcatcaa attttgactc caatccatgt gcaaatgatt gatgaaactg
     6301 gtcgtacgct attgcttcca attcttgcga tggctggtgc aggacaagtg ggtgcagcat
     6361 tggcattatg gattcgctgc aaaaaagata aagaattaac agaaatgatc aaaggagctt
     6421 taccagtcgg gatcctagga atcggcgaac cattgattta cggagtaaca ttacctctag
     6481 gacgcccatt tattactgct tgtatcggtg gaggtatcgg cggggcagtg atcggaatga
     6541 tcggaaatgt cggggctatc gctattggac cttcaggagc cgctttgatc ccattgatct
     6601 ctgacggtaa atggttaggc tacatccttg gattattggc agcttatgct ggtggcttcg
     6661 ttgctacatt cttcttcggt attcctaaag accgtttaga aaaagaagaa ttagcgactg
     6721 aaacagccac agtaaatgaa gtgcctgaaa aagtagtacc tcaaacttca gctgaaagtg
     6781 tgactatcct ctcatcagtt gctgacggaa cagttgtttc tttagataaa gcaagtgatc
     6841 cagtttttgc acaaaagatg atgggtgaag gctactttgt agaaccagaa aacggacata
     6901 tctattctcc agttactggg gtagtcagct ctgtcttccc aacgaaacac gctattggga
     6961 tcacaacgcc aaatggttta gaaatcttgc ttcatatggg aatcaacaca gttgacttag
     7021 gcggtacgcc atttgatgtg aaagtaacag aagggcagca agtgacacct gatacgcttg
     7081 tagcagaagc tgacttagaa gcgatcaaag cagcaggcaa agaaacatcc atgatcgtct
     7141 tggtaaccaa tatggatcgt gtaaaaaact ttgtcttgga aaaaacaggt aaagcaaaag
     7201 caaaagcacc tgtgatggat gtcaaagctt aatatgatag aaacaataga aacgaaagaa
     7261 gaaactgaaa tctatttttc agcttcttct tttttattat ttattgtttt ctgctaaaat
     7321 ctgataaatt cgtgtcacat cagatggtgt tccccgtaat tcataatctg ccaagaaagg
     7381 aaagccaaat gtttgtgcat attgtttggc tgttagacaa tactgattat tgaagttttt
     7441 atttccgcta ccgactacac caagacaaag tttatgattg tcttcatgtt ccagatattc
     7501 acgcatcgtt tcggtcaaga tttctgtatc accgttatct aaaccatttc cgccgtctaa
     7561 ataagtagga acgaaagtaa aaaatggttc tgtttctttc gtaaaatcac tattttcatg
     7621 gatttctttt aaagtaactt caggattgac aggatcttct gcatgcattt tttcagcata
     7681 ttctgctaga tgtttggcaa aggcacgcgt atttcctgaa atcgatatat ataaaatgtt
     7741 catggatcat cctccaattc tttcctttta gtatagcatc ttttttaagt tttttggtgg
     7801 tgtaatttgg aaagggacta ggcgctttgc ttgtataatg agaaaaaagc cggtagaatt
     7861 atatttctat taaagttttt attaattaag taaaaataaa aaaggtttgt tatacttttt
     7921 gataaaagcg gttttatttt ggaggatgct tacatggata cagagcaaca gaaaaaagga
     7981 attggtttaa ttccacttgc tgccttagta attggttcag cgattggagg tggcgtattt
     8041 ggtattatga cggacatctc cggatcggct ggcggcccag ctattttcag ttgggtactc
     8101 gtaggaacag caatgttgat gctctccttg tcgatcaata atatcaataa aaaacgtcca
     8161 gatttaaaag gcgggatctt tagttacgca gaagaaggtt ttggccgttt cgctggtttt
     8221 atcagcggtt ggggttactg gctcacatgc tggttaggca acgtagcttt tgcaacattg
     8281 ttgatgagcg cattaggata tttcttccca gtatttgaag gtgggcaaaa tctggcatcg
     8341 atcgtgatga gtacagtctt tttatgggga tatgcatggc tagtgaatcg gggagtcgaa
     8401 aatgctagtt tcgtcaatgc tatcgtcaca ttctgtaaat tagtgccatt atttttattt
     8461 attgtagtag ctatcagcac gttttcgatc caacaattca ttgataactt ttctagcgac
     8521 attatattga atgcgaatgg acagccgatc ccgttttcca gtcaagtatt gagttgcatc
     8581 atggtgatgc tttgggtatt tgttggtatt gaaggagcct ctgtcgtagg ttcacgagca
     8641 aaaaaacaat cagatgttgg gaaagcaacg attctaggga tattcggctt gattattatt
     8701 tacgtcttgg tttccatgct tccatatggg acaatgacga tgaatcagct gcaaacgatc
     8761 aaggcgcagc ctgctatggg ctatgtgttt gaaatgatgg ttggtaaatg gggagcagtc
     8821 gtgatcaacg gcggtttgat catttcttta gttggtgttt ggctctcatg gacgatctta
     8881 ccgattgaaa caatgggaaa catggctgaa gacggacttt tgcctaaagc atggggaaaa
     8941 ctcaacaaaa atggggcacc aacttatgct atcgtattga cgacgttatg tacgaatgtc
     9001 tttttacttt cattattatt cactgacagt gcttataact ttgcttacac acttggtaca
     9061 gcagctattt tcttcacgtg gcttttctta ggattgtacc aaatgaaact ttcttatcag
     9121 cggaaagaat gggtccaatt tttagtaggg cttttagcaa gtgcctttca agtatgggct
     9181 atgctagtcg tagcctttaa tgaagtaatg cttgttcttg ttcttttctt gccaggcatt
     9241 tatttttacc tcaaagcacg taaggagcaa aacgagcctg tacaacatat ccacttagat
     9301 aagattttac ttataggtgt gacgatggtc ggagtgattg caatggtttt atttgccact
     9361 gggatgcttc acgtatagaa gtataaaaga agtataaaga gaggttgtga gcctgtcgtt
     9421 tattcaaaaa catactagga aaggagctgt gacacaaatg ttgtcacagc tcctttttca
     9481 gaataaacgg tgtttaagag acagccaatc ggcaataagg ctgaacgtct ctttcacagc
     9541 ctcttttttg atgagtcatc atacattttt tcgcttaaat acgagatatc ctaatgctaa
     9601 gaacaaaacg atgtaagcta aattaccgaa tagcatttga ttagtagaaa gatgagtcaa
     9661 ttgcttcatg atctcttcgt ttccaatttg gttttgcaga ttcaacatac tgatcggatt
     9721 ccatttgatc cactcccatt tttggatgag tgcaataagg ataccagata tgattgacga
     9781 agcaaaataa aagacgattc ctgctgagat ggatgcgccg gaactattga taaagcaagc
     9841 aatcatcaac actaaactta aaatcagcca taaaccgata taactaccca aagtataagt
     9901 gaacaagctt tggatcagtg tttgaccagt agaaaccttc tccgtgaagg aaatcgaagg
     9961 gaaaagaatc agtttcatca acacagtgac aataatagct aaaaggtata aataaacaga
    10021 ataaataact aatgtgatcc atttgcttac taatatttct cctcgagaat actttcgata
    10081 aaggagattt ttcaatgtac catattgtgc ttccatcgaa atgatcgtgc ttgcagctgc
    10141 aatcatgata aagacaatcc aagatgtggc actaaaaagc tgagcagtca tttcaactgg
    10201 atcaatgatc gtcgtgtatt ttttggctag aagtgctgtt cctatgagca aaacaactaa
    10261 tagaacagat aaaacagcag ttgacttttt cttgatcaat ttaaataatt cttgttttac
    10321 aagcaatgtc atttttcttc ttcccccttt actgaatcca gaatttccaa tagagaagat
    10381 tccaaatcat tcgtcagatg ctggacgtct gtcacatgga tattttgtgc agacaatagc
    10441 tggatcacgg ttcctaataa ttcttcgttt tcttttaaaa gcttgatttt atcaggatct
    10501 tccaaagtat atccagcttc tgtcaagatc gttcgagcag ctagatcatt tgttgtagag
    10561 ataacgatga aatcttgcgc tgtttggttc aattcagcca ttgtacattg tttgatgatc
    10621 tttccttgat tgatcaggat cagctcatcg accatttttt caagctcgct gagtaagtga
    10681 ctggagatca agaaagaggt gccctgagta gccaagtcgg taatgatttt tcgcacatct
    10741 cttgttgctt gtggatccaa accgttcatc ggctcatcta aaataacgat agacggatgg
    10801 ttcagtaagg caagagcaat tcccaatttt tgtttcattc ccaacgagta ggatttcgct
    10861 tttttgtgaa tgtacttttc catcttgagc tgattcacga tagtgtcgat agcagataca
    10921 tcgttggttt cagagaataa cttcagatga tcatagcctg ttaagaatgg atagataccc
    10981 gggtattcga tcaatgctcc tacttgttcc aaccctttat gactagaggg agaaatctca
    11041 ttttcaccaa tacagattgt tccatctgag taatggatca gtcctaaaat agctttcatg
    11101 atcgttgttt ttccagcgcc attaggacct actaatccaa cgattttgcc aggggataag
    11161 ctaaaactga catcgctgag ggcttgtttg tgaccaaagt tcttggataa ttcttggacc
    11221 gaaagacttt tcaaaaatat tcactccttt tctatagata gtacaagcat acacctgatg
    11281 agtggagaag tcgctaggtc attggactta tttcttattt tttcataaaa aaattgaata
    11341 ttaaataaaa aggcttattt tttctgatca gtacttattc caagtgtcta gtggccgcta
    11401 gtaatcttga tgccaatgat actgatgagt agcatcccca caaagaacca agtggaaaga
    11461 ttcagcttat catgaaacaa gaccacacca attatgaccg aaccaactgc gccaatccct
    11521 gtccagatag gataagagag actcatcggc aaacttttga ttgcctttga caaaaagtaa
    11581 aaactagcaa tcatgccaat cactgtgtaa aaagaataat tgattttaga aaaaccatca
    11641 ctccatttca tcatcgttga ccagaatact tccaaaattc ctgctatgat taattccatc
    11701 catgccatac ttacattcct ccttaactat aaaaaaatac acaaaaagag gaaaatcatc
    11761 ccttcaaaga cctaatggaa tgatcatcct ctttatccta cccggtggca gattgtatat
    11821 ctttttctgt agtatagcac acctatccag atatcggcaa tctcatactt tttagtaaag
    11881 tgtaccaagc actgacggat atatcgttag cggaaaagaa gatgaatgct ttgcaatttc
    11941 atagaaagaa gttgttattt attaatcatt taaaaaaaac agtaactaga ccttatttct
    12001 atatattatc gggcaataaa ttaaaaaaa
//
LOCUS       DASNNU010000078        11998 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.078, whole genome shotgun sequence.
ACCESSION   DASNNU010000078 DASNNU010000000
VERSION     DASNNU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.078"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..828
                     /locus_tag="U8116_002101"
     CDS             <1..828
                     /locus_tag="U8116_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEN8435083.1"
                     /translation="PFDSEVKGLLNKLTISNIDSTREKIIKIFDKINNLFKNDNAELR
                     KGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQ
                     FNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVY
                     VISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLH
                     NHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            900..2021
                     /locus_tag="U8116_002102"
     CDS             900..2021
                     /locus_tag="U8116_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN8435084.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(2103..3875)
                     /locus_tag="U8116_002103"
     CDS             complement(2103..3875)
                     /locus_tag="U8116_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8435085.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(3878..5590)
                     /locus_tag="U8116_002104"
     CDS             complement(3878..5590)
                     /locus_tag="U8116_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8435086.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            5705..6373
                     /locus_tag="U8116_002105"
     CDS             5705..6373
                     /locus_tag="U8116_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEN8435087.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(6440..7228)
                     /locus_tag="U8116_002106"
     CDS             complement(6440..7228)
                     /locus_tag="U8116_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEN8435088.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7311..8783)
                     /locus_tag="U8116_002107"
     CDS             complement(7311..8783)
                     /locus_tag="U8116_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN8435089.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(8913..10106)
                     /locus_tag="U8116_002108"
     CDS             complement(8913..10106)
                     /locus_tag="U8116_002108"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEN8435090.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(10104..10183)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(10372..10443)
                     /locus_tag="U8116_002109"
     tRNA            complement(10372..10443)
                     /locus_tag="U8116_002109"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10408..10410),aa:Glu,seq:ttc)
     gene            complement(10455..10528)
                     /locus_tag="U8116_002110"
     tRNA            complement(10455..10528)
                     /locus_tag="U8116_002110"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10492..10494),aa:Ile,seq:gat)
     gene            complement(10541..10611)
                     /locus_tag="U8116_002111"
     tRNA            complement(10541..10611)
                     /locus_tag="U8116_002111"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10577..10579),aa:Gly,seq:tcc)
     gene            complement(10616..10688)
                     /locus_tag="U8116_002112"
     tRNA            complement(10616..10688)
                     /locus_tag="U8116_002112"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10653..10655),aa:Phe,seq:gaa)
     gene            complement(10692..10764)
                     /locus_tag="U8116_002113"
     tRNA            complement(10692..10764)
                     /locus_tag="U8116_002113"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10729..10731),aa:Asp,seq:gtc)
     gene            complement(10783..10856)
                     /locus_tag="U8116_002114"
     tRNA            complement(10783..10856)
                     /locus_tag="U8116_002114"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10820..10822),aa:Met,seq:cat)
     gene            complement(10869..10958)
                     /locus_tag="U8116_002115"
     tRNA            complement(10869..10958)
                     /locus_tag="U8116_002115"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10920..10922),aa:Ser,seq:tga)
     gene            complement(10981..11052)
                     /locus_tag="U8116_002116"
     tRNA            complement(10981..11052)
                     /locus_tag="U8116_002116"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11017..11019),aa:Glu,seq:ttc)
     gene            complement(11074..11161)
                     /locus_tag="U8116_002117"
     tRNA            complement(11074..11161)
                     /locus_tag="U8116_002117"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11125..11127),aa:Ser,seq:gct)
     gene            complement(11186..11259)
                     /locus_tag="U8116_002118"
     tRNA            complement(11186..11259)
                     /locus_tag="U8116_002118"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11223..11225),aa:Ile,seq:gat)
     gene            complement(11273..11343)
                     /locus_tag="U8116_002119"
     tRNA            complement(11273..11343)
                     /locus_tag="U8116_002119"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11309..11311),aa:Gly,seq:tcc)
     gene            complement(11345..11420)
                     /locus_tag="U8116_002120"
     tRNA            complement(11345..11420)
                     /locus_tag="U8116_002120"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11385..11387),aa:Asp,seq:gtc)
     gene            complement(11439..11512)
                     /locus_tag="U8116_002121"
     tRNA            complement(11439..11512)
                     /locus_tag="U8116_002121"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11476..11478),aa:Met,seq:cat)
     gene            complement(11525..11614)
                     /locus_tag="U8116_002122"
     tRNA            complement(11525..11614)
                     /locus_tag="U8116_002122"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11576..11578),aa:Ser,seq:tga)
     gene            complement(11638..11711)
                     /locus_tag="U8116_002123"
     tRNA            complement(11638..11711)
                     /locus_tag="U8116_002123"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11675..11677),aa:Ile,seq:cat)
     gene            complement(11725..11798)
                     /locus_tag="U8116_002124"
     tRNA            complement(11725..11798)
                     /locus_tag="U8116_002124"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11762..11764),aa:Met,seq:cat)
     gene            complement(11826..11898)
                     /locus_tag="U8116_002125"
     tRNA            complement(11826..11898)
                     /locus_tag="U8116_002125"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11863..11865),aa:Ala,seq:tgc)
     gene            complement(11918..11991)
                     /locus_tag="U8116_002126"
     tRNA            complement(11918..11991)
                     /locus_tag="U8116_002126"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11955..11957),aa:Pro,seq:tgg)
BASE COUNT         3886 a         2531 c         2086 g         3495 t
ORIGIN      
        1 ccatttgatt cagaagtgaa aggattactc aataaactaa ctatttctaa tatagactcg
       61 actagggaaa aaatcataaa aatatttgat aaaattaata atttattcaa gaatgacaat
      121 gctgaattaa ggaagggtct tttagagttt aaacttcaag aactagaact tcattactct
      181 tatttagtga aaattaccga agaaaaagaa caacaaaaag caataaaaga acagatgata
      241 gaagaggaaa aagttcgtag agaaattgat cgtgaaaaga aacgtattga taaagaacaa
      301 cgtcaattca attctgaaat ttccaaatta atcgcatata tgcaaaaagc taatgcagat
      361 gtggaaaaag aattatatgc aaataaaatt caagaactgg aagaaaaatt aaaagaatta
      421 gaagttatac gtgaaaatgt attacaaaga gaattaaata ctagagccgg ttatgtgtat
      481 gtaattagta atattgggtc ttttggtgaa gacatatata aaattggtat gactagaaga
      541 ttagaaccta tggatagaat aaaagaattg agtagtgcat cggttccatt tgaatttgac
      601 gttcatgcga tgattttttc agaggacgct ccgtctttgg aaactaaatt gcataatcat
      661 tttagaaaac aagaagttaa taaaattaat caaagaaaag aatttttcaa agtttcacta
      721 gacgaaatag agaaagttgt tttagaaaat tataatggaa cagtaacatt tacaaaatta
      781 gctaaagctg aacaatatag acgatcttta gaattgtcaa aagattaaat atataaaaag
      841 ccttcgggct ttttctttta acaattaata ggaacgtata ttcgaaagga atagaaataa
      901 tgtggttaga aaaaataaat gacaaaaaat ttaagtacac cgaacgttat acggatccct
      961 taactgaaaa aaagagaaaa gtgtccgtta ctttatctag caattcacgc caagcttgga
     1021 atcaagctaa cttacttttg aatgaaaaaa tcgcagaaaa aataaaacga aatgaagaat
     1081 taccactaac ttttggagaa ttaaaaacta aatgggatga aaaatacaag ccaactgtaa
     1141 aagaaagttc ctatcgaaca actcaggttt atctatcatt aatttcaaaa tatataaaag
     1201 atgatgtact tgtaaaaaac gttaatagta atcttataca agatatgcta gattactact
     1261 attttgaaaa aaatttatca tataattatg taatacattt aaaaactttt acaggaatga
     1321 tttttaaatt cgccaaccgt cgatatggat taaaatataa tcccgtaaat ggggtttctt
     1381 tagcaagaaa acctaagaca aacgaagaaa taaaaaaaga aaaagatggt tatttatcaa
     1441 aagaagaagt tcaaatgatt gctagtaaac aaaaaagttc acatcaacag tcacgttatt
     1501 ctttaattac taaatttttg tttttaactg gactacgtta tggtgaattg atatctttga
     1561 ctgaatctga ttatgatggt aataaaatta cggtttctgg tacttatgat tacgagttaa
     1621 aaattaaaac aacaactaaa aatacaggtt cttatagaac cattgaatta tcaagtaatg
     1681 ctaaagaaat catagatcaa ttaattgaag aaaacaaatt aattaagaat aataaagata
     1741 aatacatttt catctctaaa aatggtaacc cgatatctat tcaagcatat aatcaatctt
     1801 taagagcagt atccaaagaa ttaaacctag ataaaaaagt ttcttctcac atgttaaggc
     1861 attcccacat ttctctactt acagaattag gaatcccatt aaaagccatt atggatcgtg
     1921 taggacatga agattcaaaa acaacattaa agatttatac tcatacaact aaaaatatgc
     1981 aaaatcaact agtagaaaaa ctaggaaaaa tagaaattta aattgcccct tttctgcccc
     2041 ttataaaaac aaacaacata aaatatataa aaagaacccc gttataacga ggttctttta
     2101 atttaacttt caaatacaaa ttgattatga taaagttccg cataaaatcc attttctgct
     2161 aataactcgt gatgactgcc ttcttcaatg acttccccat cacgcaagac aacgattcta
     2221 tctgcattca agattgtttt cagccggtga gcaatcacaa agctggttct tcctttgatt
     2281 gcttcatcca ttgctttttg gatccttgct tctgttaccg tgtctacatt acttgttgct
     2341 tcatccaaaa tcaataaaga tggatttgtg atgatcgttc gagcaatact gatcaactgc
     2401 ttttgacctg tactgaataa gttattttct tcggatatct ctgtatcata acctttttct
     2461 aacgtcatga tgaagtcatg aatattcgct tgttttgctg cattgatgac ttcttcatct
     2521 gaagcgtccg gtttgccgaa tgtgatattg tctttgatcg ttccggagaa caatacagat
     2581 tcttgcaaaa cgattcctac atgtctacgt aatgcatcaa gatcataatc tcggatatct
     2641 attccatcga tgactactga accatcattg acatcgtaaa aacggtttag cagattcatg
     2701 atcgtcgttt tccccgatcc tgttggacca acaagagcaa ccatttctcc tttgtccaca
     2761 ttgatcgaga catctttcaa gactagtttt tctggagaat aaccaaaatc tacgtgctgc
     2821 aacgcaagac ctttttcaac accatcaaat gctttacagt tttctggttt gacttcatct
     2881 ttttcatcaa atatttcatt caatcgtctt gcccctgtaa tcgctaattg gatcatgctg
     2941 tatccagatg aaatctgcat cagtggttgg taatattgtt gtgaatactg gacgaatgtc
     3001 acaactaagc ctaatgccac cgaacgttcc aaatcaccat ttaatgctaa ccagccgccg
     3061 aaaaagataa ctatcgctgt attgaccagt gacattcctt gcatcatcgg aaatagtaga
     3121 ccagaataag cttgtccttt gaaggttgct tcgcgaacac tttgattatg ctctaagaat
     3181 ccttcaatcg tttcttcttg taagccattt gtgatgatga cacgttgtcc gctgattttt
     3241 tcatccatat aaccatttag tttccccact tcatcttgct gaagatccac atatttccgt
     3301 gctttgcgga taatcaagac agctatcaaa acggctactg gagtggaagc aattgtagcc
     3361 caagctaact ctacattttg gcggaacatc atgatcaaga tcccgacaag taaaacagca
     3421 ttcgtgatga cttgaagcaa agcttggttc aaactatttt gaatattatc taagtcactg
     3481 gtaaaacgac ttaaaatttc accgtcttga tgagaatcaa agaaacggat cgtcaatttt
     3541 tccaatttgt tgaatagacc gatacgcatt cggttcgttg acttccctac tacttgtgtg
     3601 aacaaaatac tatagataaa gttggctgca ctagctagta cataaaacag caagagtttc
     3661 catattacag aaacaaattt gcttttatcg tctacgcctt gcatcaagcc tcctacgtaa
     3721 ttagctaatt cttgaaaagc ttccccgatg tattgaggtg cctttacttg tagataggta
     3781 gcaatgatca ctgtgataaa gataaagaaa aaggagagtt tgtaccgctt caaatagtga
     3841 tagaaaaatt tacttgcttt tattagatca gtcatactta ctcctccgtt cctttttgag
     3901 tttcaaagat ttcctgatag acacggtttc cagctaccaa ttcctcatgg gttccttcgc
     3961 cgactaatct gccttcatcc aatactaaga tgcggtctgc tttgacaaca gaagcaattt
     4021 tttgtgcgat gacaatcgtc gttgttccct tcaaatctcg atcgagtgct tctcggacca
     4081 gcttttcaga gcgagcatcc agtgcactcg tactgtcatc taggatcaag atctttggat
     4141 taccgataac gccacgagta atggataaac gttgcttttg accaccagaa aagttactgc
     4201 tacgttcttc tattggtgct tcgtaacgat cagataactt ttcgatgaat tcttttgctt
     4261 gagcaataga agaagcatgt tccatctctt tttctgttgc attctttttc ccttggcgca
     4321 agttttgagc aatcgttcct gagaataaaa ttgctttttg caaaacaaag gaaaccgttt
     4381 gtcttaaact cttttcattt acttgacgaa gatctacacc acctacttca actttgcctt
     4441 ctgtcggatc aaatagacga gggatcaatt gtgctagtgt tgacttcccg gcaccagtcg
     4501 ccccgacgat accaaccatt tctccaggtt tgatagaaaa gctgatatct ttaagcgtat
     4561 ctgtatcctc accaggataa cgaaaagaaa catggtcaaa agtgacagaa cccgtcaact
     4621 cttgttctgg aacgtctaaa taagtaagat ctggttcagc atctaaaatt tccgcaatcc
     4681 gcttcagcga tacggctgct cttgaagtca tcatcatcat cattccgcca ataatgattg
     4741 ccatcataat ctgcatcaag tagttcatga atgaagcaat tccaccaatt aacgtaggat
     4801 catctttaac aagatcgctc acaaaaaaga tcgccccaac cacagccaaa ttggcagcca
     4861 gcatgaagga cgggatcatc acagaaaata atgtaccgac tatgatgttg tgttttgtta
     4921 agtcctcact tactttgctg aatgacttca attggttttc ttcttgtaca aaagatttca
     4981 ctacacgaat ccctagtaag ttttcttttg caatgctatt cacacgatca atcagttttt
     5041 gaatgatcat gaagtgcttg cccatccttg taaatgaaag tgccgtaatc agaaataccg
     5101 taacgataag cgctacgatg atccaccata attgcggcat cgtgatcata gctaaaatga
     5161 aggcaccaat aaataagaat ggaattcgaa aaagtgattg caaagaaatc atcactaaat
     5221 tttggatttg cgtgatatca tttgttagac gaacaacaag atttccagca gagaactgtt
     5281 cgatatttcc aaatgaaaat gtctggatct ttcgaaatgc ttcttctcgg atatctgcac
     5341 ttacgccttg agctactttt gcagagaaaa tcgtgttcgt tacacctgcg gctaatccta
     5401 gcaaagccag actgatcagg taaataccaa ttgttttcat ttcatcatta tcttctgtaa
     5461 taatagcttc caatacttgc tgtagtaact taggttgcca taatgctgct gcaaccatga
     5521 ctgctacgga cagcaaggaa ataaaaacag ctactttata ttttttcgca tgtcgtaaga
     5581 ttaatttcat tcgttgatta cacccctttg tttaaattga caggactaat ttaacactgt
     5641 aaaattagac cgactaacaa tttaccatgt ttaaaaagat aatgcaatac acgaggttga
     5701 taaaatggaa ataaagcttt ctcaagcaat catcattcag gccgcctttg atatcctggc
     5761 tgaaacacaa gctctttcgc agctttccat gcgaaacatc gcaaacagaa ttcatgttca
     5821 agcacctgct ttatattggt atttcaaaaa caaacaacag ctcttgcaaa taatggctga
     5881 aacaatggaa gaagaattag cgcttccaga tccaactttg ccttggaatg aacagttgct
     5941 gcagtatatg gaaaattact atgaccttta tacacgtttc ccttgtgggg cagaattaga
     6001 aatcaatact gtgcccgctt ttccgagtcg attagaacat ttggagcaaa tgatccaatt
     6061 acttgttcgt gaaggatttt cagtcaccca aagtcatcag gccattgcct ctctccacaa
     6121 tttattgatt ggacagttaa tggatcagca gcatgaacag cagcttcgac aaaaaattat
     6181 ggacggaaat gaatttttga aagaaacagt tctatttatg cgtaattatg tagttgaaaa
     6241 tcaattagag ggatttaaag aaagtatgca gtcccatgca gaattgaatg aaaaagcact
     6301 ctttctaacg agtgcacaaa cctatattga aggcttatgc caacaaaata aaagaagaga
     6361 aaacgagcag taaaaaagct cctatagaga tcactatgtt tgtgatctct ataggagcta
     6421 tctttttaag actctttttt tatttttctt ccattagcca cgccaatgca cgttgaatcc
     6481 attggtccca gaagatccag ttatgatcgc caggtccttg ttcaaaggtg accgccaagc
     6541 cttgttcctg catagcagat accatttcct gatttgcctg ataaagaaaa tcagattccc
     6601 cacaagccat ataaaatctt ggaactggtt ttccgctttt tactagttct ttcaataagt
     6661 attgcggatc attttttgaa ccttcgattt gatccaatgg tccaaagatc ccttcccaaa
     6721 aagatgcttt acgaaccatc aaaagatcat ccattcgatt tcctacagaa acagcgcctg
     6781 aaagagagat tactgctgcg aaattttctg attttgcaag gcctagtttt aacgcgccat
     6841 atccgcccat cgataatcca gcggcatatg ttttttctct ttttcttgtt aattgcggga
     6901 ataattcatg acaaatctca ggtagctctt ctgcaatgaa tgtccaatac ttcatgtcat
     6961 aaactgtatc tgtgtaccag ccaagatcag tagatggcat gatgacagct aatccgtagt
     7021 ttgctacata acgttcaatc gacgtacgtc tttcccatac actatgattg ccacccattc
     7081 catgaagtaa atataaaaca ggtatatccg ttgtctcttg ctctgaactg gtgccaatca
     7141 ccttttttgt tgtttgcggt agtatcacat ttagattgac ttccatttct aacacattcg
     7201 aataaatatt tgcttgaata aatgccatat tttcgccttc cttagtaaga agttattttt
     7261 gaataacttt aaatttgaat gattcggcta cgtacatatc ttaaccgaaa ctactgtttt
     7321 attttaccac ttttttcatg aacgaaaagc gttaagacca aagcaatgat cacgagtaac
     7381 aagccaacta gataaacgga atgtaaccca tcataaagaa tttttcttaa tggtaacaaa
     7441 atatccgcat caatatattt agccgtttgt ggattcacca gtttattcat gatatctgca
     7501 tcatttgtta aatttgctgc tgcaagttct cgtttagtta ccccatttat caataaacca
     7561 aaaatagaaa ccatgactgt ttgaccaatt gttcgaacta acgtattaaa agaagttgct
     7621 acaccaagct ggtcacgaga tacactattt tgtgcactga cagtcgaggt agtcatggtc
     7681 acacctaagc ctataccgat gaccgttgat atagcaaaaa accataaata gttacttctg
     7741 aaaggcataa gatacaacca agcgccccca atagctgcga ttagtagtcc aattctcaag
     7801 acccaatgag ttttcatttg agtcatccac ttgcttgcaa ggaaagaacc gaccatccag
     7861 actagtgata atggtgctaa taccagccca ccaattgctg cattcagccc taatacacct
     7921 tgcatccaca tagggatata aacatcgatt cccatcaaaa aaccactagc caatgctgca
     7981 gccaagttga ccacaacaaa tagtggattt ttgaataaat gcatcatgat gacaggatct
     8041 ttagctcttt tttcggcaaa aacaaaaata cctaacatta cgatactacc tagcagcaaa
     8101 gcagaaacaa gcggactcat tccttcgtct cctaataatt ggaaccctaa cagcaatgag
     8161 acaagcatgg ccatcaaact aaaactacca aaaatatcca ttggtttaga ctcatgtatg
     8221 atttttggtt catttaaata tagccaaatc aaaataatta atagaattcc gatcggcaca
     8281 ttgataaaaa agatccagtg ccagctgatc gtttcaacga tgaatcctcc cgcaagcggt
     8341 ccaaaaacac tggcaatccc ccaggctgaa ctattcaaac ctaaaacttt tgctcgcttt
     8401 tcaatagaat aaagatcgcc gatgatcgtc aaagctactg gcatcattgc cccagcaccc
     8461 atcccttgaa tcgcacgagc agcaatcaac ccaatcatgc tttgagataa accgcaaaat
     8521 aacgaaccga taataaacaa taatgtccca aaaataaaga ttggtttccg tcccacttta
     8581 tccgcaagtt ttccgtaaat cggagtaagc atagcgtttg tcagcaaata aatcgaaaat
     8641 acccagttca tgatttccat tccatgtaat gaaccgacaa tcgttggcat agcggttgtt
     8701 acgattgtac cttcaatagc cgtcatgaac gttgcaatga aaatactcac tgtgaccaaa
     8761 gtaacatttg tttctttttt catcttattc ctccctttca ttatgacagg atctaaaaat
     8821 agataaataa ttactatctt tttcaagagg ttaactgcca taaaaaaagc tggaacaacc
     8881 accacttggc gatcgttcca gctgaatata acttattttg ctaaacttgc tttcaacgca
     8941 tctactttgt ccgtatgttc ccaaggcaaa tctacatctg tacgaccaaa atgaccgtaa
     9001 gcagctgttt gtttgtaaat cggacgacgc aaatcaagca tctcgatgat tcctgctggc
     9061 cgaagatcga agttttctct cactgcagca atcagttctt cttccggaac cgttccagta
     9121 ccgaaagtat tgattgagat cgaaacaggt tgagcaacac caatcgcata agctagctgt
     9181 acttctgctt tgcgagcaag ccctgctgca acaatgtttt tagcaatata acgtgcagca
     9241 tagctggcag aacggtcaac ttttgtcgca tctttaccag aaaaagcacc gccaccatga
     9301 cgagcataac cgccatacgt atcaacaatg atttttcttc ctgttaatcc ggcatcccct
     9361 tgaggaccac caatgacaaa tcggccagtc ggattgatat aatatttcgt ttgatcatct
     9421 aataattcag ctggaataac ttccttcact actttttcga tgacatcatg acgaattgtt
     9481 tcattatcta ctgcatcatc atgctgtgtt gaaataacga ttgtatctac gcgttccggt
     9541 tgtccttgat catcatattc aaccgttact tgagattttg catctggacg taaatacgtt
     9601 aattcatttg atttacgcaa atctgccaag cgtcgtacca gacgatgact caaagcgatt
     9661 ggtaaaggca tcaattctgg tgtttcatct acagcaaacc caaacattaa accttggtca
     9721 ccagcaccaa tctcatctaa tacgtctttt ttatcttcat cccggatctc aagtgcttca
     9781 tcaactcctt gagcaatatc aggagattgt tcatcaatcg caaccaatac agctgctgta
     9841 tctccatcaa atccgaattt tgcacgtgta taaccaattt cttttattgt ttcccgtacg
     9901 actttttgaa tatctacata tgccgtcgta gatatttctc caaatactaa aacaagacca
     9961 gttgttacag acgtttcaca cgctactcgt gctgttggat cttgttttaa aattgcatcc
    10021 aagattgcat cactgatttg gtcagcaatt ttatctggat gtccttcaga aacagattct
    10081 gatgtaaata agtgtctttc taccattata atgattcccc ctagttaaat tcggttacaa
    10141 ggcatctccg tcctgcggat cctttcggga acttgcaacg aaccaagtat agcagataaa
    10201 acagttttct gctcgtattt tttcattttt ttgcttaaaa aatataaaaa tattttacat
    10261 tagaactgtc caaaaaagct tagcacaatc atccataaag ataactctgc taagcttttt
    10321 aaaatcattc tttcagataa aaagaaaaag atactcaaaa agtatcttcc atgacccgta
    10381 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    10441 gccaataaaa taaatgggcc taaatggact cgaaccatcg acctcacgct tatcaggcgt
    10501 gcgctctaac cagctgagct ataggcccat aaaaactaaa agcgggtgac gagaatcgaa
    10561 ctcgcgacaa cagcttggaa ggctgtggtt ttaccactaa actacacccg cataatggcg
    10621 cgggacagaa tcgaactgcc gacacatgga gcttcaatcc attgctctac caactgagct
    10681 accgagccaa acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca
    10741 tgttaacccc tacaccacgg aaccaatgtt tttagttttt aattgcggga gcaggatttg
    10801 aacctacgac cttcgggtta tgagcccgac gagctaccag actgctccat cccgcgataa
    10861 tatgaaaaag gaggataagg gattcgaacc cttgcacggt tttacccgcc tgacggtttt
    10921 caagaccgtt cccttcagcc ggacttgggt aatcctccat gtaaaactat attaaattaa
    10981 tgacccgtac gggattcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt
    11041 gaccaacggg ccaggtaaaa caaaatattg aaacggagaa ggagggattt gaaccctcgc
    11101 gccggtttcc cgacctacac ccttagcagg ggcgcctctt cagccacttg agtacttccc
    11161 caaaaccaat aagattttgt actaatgggc ctaaatggac tcgaaccatc gacctcacgc
    11221 ttatcaggcg tgcgctctaa ccagctgagc tataggccca taaaaaacta aaagcgggtg
    11281 acgagaatcg aactcgcgac aacagcttgg aaggctgtgg ttttaccact aaactacacc
    11341 cgcatggcgg ttccgacggg aatcgaaccc gcgatctcct gcgtgacagg caggcatgtt
    11401 aacccctaca ccacggaacc agtgttttta gtttttaatt gcgggagcag gatttgaacc
    11461 tacgaccttc gggttatgag cccgacgagc taccagactg ctccatcccg cgataatatg
    11521 aaaaaggagg ataagggatt cgaacccttg cacggtttta cccgcctgac ggttttcaag
    11581 accgttccct tcagccggac ttgggtaatc ctccatatga aacttacaac gccacaatgg
    11641 accttgtagg actcgaacct acgaccggac ggttatgagc cgtctgctct aaccaactga
    11701 gctaaaggtc caggctctta aaaatcgcgg cggaggggat cgaacccccg acctcccggg
    11761 tatgaaccgg acgctctagc cagctgagct acaccgcgaa aaagatattc attatttaaa
    11821 tgaaatggag cctagcggga tcgaaccgct gacctcctgc gtgcaaagca ggcgctctcc
    11881 cagctgagct aaggccccgt gtttaaattt ataattatcg ggaagacagg attcgaacct
    11941 gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc gtttaatt
//
LOCUS       DASNNU010000079        11989 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.079, whole genome shotgun sequence.
ACCESSION   DASNNU010000079 DASNNU010000000
VERSION     DASNNU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.079"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            85..2103
                     /locus_tag="U8116_002127"
     CDS             85..2103
                     /locus_tag="U8116_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN8435091.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2173..2379
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2530..4944
                     /locus_tag="U8116_002128"
     CDS             2530..4944
                     /locus_tag="U8116_002128"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEN8435092.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5292..6845
                     /locus_tag="U8116_002129"
     CDS             5292..6845
                     /locus_tag="U8116_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN8435093.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7226..9010)
                     /locus_tag="U8116_002130"
     CDS             complement(7226..9010)
                     /locus_tag="U8116_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8435094.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(9025..10746)
                     /locus_tag="U8116_002131"
     CDS             complement(9025..10746)
                     /locus_tag="U8116_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8435095.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10846..11607
                     /locus_tag="U8116_002132"
     CDS             10846..11607
                     /locus_tag="U8116_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN8435096.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3931 a         2168 c         2427 g         3463 t
ORIGIN      
        1 acaaagctct taaataactt ttaataaata ttaagagaag ttataattat taataataga
       61 taaattgaag ttggaggata gaagatggaa aacattgcaa gaaaagaacg gagacgttta
      121 gaacagacca aacgttttcg aaaagcaaaa cgaggggcag caatcgtggg aactgcgatg
      181 gtaggctgtt ctgtcgctgc cccattagta cagccggttc ctgttcaagc ggatgaaaca
      241 cctgttcagt tcggcgcaag aatcaataca gcggcattta tcacagaaat tgctacttat
      301 gctcaaccaa tcgctcaagc caacgatcta tatgcatcag tgatgatcgc acaagcagtt
      361 gtagaaagcg gttggggcag tagtgcgtta tctcaagcac cttattataa tctttttggg
      421 ataaaaggca gctatcaagg acaaaccgtc tacatggata cgcttgaatt tttaaataat
      481 caatgggtaa ataaaaagga gcctttccga caatatcctt cttttgctga atctttcaat
      541 gacaatgcct atgtacttag aaatacttca tttggcaacg gttattacta tgccggtact
      601 tggaagagca acacgaaatc ctatacagat gcgacagctt gtttgacagg gcgttatgcg
      661 actgatcctg gttacgcagg caaattaaat aatatcatcg caacttacgg attgacccaa
      721 tatgatacac cagctagcgg aaacgcaggc ggtggagcga ctacaggtgg caataatact
      781 ggaagtgcag gtagtactgg cggttcaagc aattctggta atacaaactc aggttcagca
      841 acttcgacca cttatactgt ccaatctggt gattctgttt ggggaatctc aaataaattc
      901 ggtatcacaa tggatcaatt gatccaatgg aacaacatcc aaaacaattt catttatcca
      961 ggacaaaaac tgacaatcaa aggtagccag gaaaatggct cttcgacgaa taattccggt
     1021 aacaatacca attcttcagg aaatgctggt acatcaaacg gaggacaaac aactggtgcg
     1081 aaatacactg ttcaatctgg tgattctgtt tggaagatct caaatgatca cggaattacg
     1141 atggctcaat tgattgaatg gaacaatatc aagaacaatt tcgtttatcc aggacaacag
     1201 ctaatcgtat caaatggcgg ttcttctaat gctggagcaa atactggctc aaatactggg
     1261 aatacaagtg gctctagttc gtccagctca agttcttcta atacagcttc aggaacgaaa
     1321 tatacagtta aagccggaga ctctgtttgg agcgtctcaa ataaatacgg aattacgatg
     1381 gctcagctaa tccaatggaa caatatccaa aataacttta tctacccagg acaacaactg
     1441 actgtttcaa acagtggatc aacaaacagc acgtcaggta atacaggttc gtcaaccaat
     1501 acaaataaca gcagttcagt taatactggc agcagttcgt ctcaaggcac ttataccgtg
     1561 aaagcaggtg aatctgtttg gagcgtggca aataaaaacg gcatcacgat ggatcagtta
     1621 atcgaatgga ataatatcaa aaacaacttc atttatccag gacagcaatt gatcgttaaa
     1681 ggtggaactt cggcaaatac aaatacaggt tcaactactt ctgcagcgaa accaaataca
     1741 ccgaatacgt ctgctacgac aagtacatca tccactggta atacgatgta taccgtgaaa
     1801 gcgggcgagt cagtttggag cgtagcgaac aaacatcata tcacgatgga tcagttaatc
     1861 gaatggaata atatcaaaaa taatttcatc tatccaggac agcaagtgat tgtgaagaaa
     1921 ggttcatctc aatcaacaaa ccaacaatca gttactggtt cgaaaaccta tactgtgaaa
     1981 tctggagaat ctgtttggag tgttgctgac agtcatggaa ttacaatggc tcaactaatc
     2041 gaatggaaca atattaagaa caactttatt tatccaggtc aaacgttgat tgtagctaaa
     2101 taaaatagct gttcgaagaa ataacgattg aattccacaa aaatatttgt tactataagg
     2161 ctacttaaat atgacaacac ttgaaaagga agtagtaaaa agactgaagt ttcagagagc
     2221 gcatggctgg tgagaatgtg cacggatgct tttgaactcg cctttgagtt gcccttgctg
     2281 aatacccgag taagcaagcg gcggtgacgg ccgttacaac gttcgaggcc ttttcactaa
     2341 ggtaaaatta ggtggtaccg cgtagattac gtcctataaa aagagcaatc tttttatagg
     2401 atttttttat ttcttttgtg aagtttgaga aaacaaagca aaaggaaata aaaataaaca
     2461 gattctgccg gttttttcgt tatatttata atagaaacaa gtgttttcaa tagaacagga
     2521 ggacagcaaa tgagttacaa tcataaagaa attgagaaaa aatggcaaaa atactgggca
     2581 aaaacgaata ctttcaacac ccatgatgat cctgaaaagc caaaatttta cgcactggat
     2641 atgtttccct atccatccgg tcaagggcta catgtagggc atcctgaagg ttatacggca
     2701 acggatatcc tttcacgtgt gaaaagaagt caaggataca acgtgctgca tccaatgggg
     2761 tgggatgcat tcggtctgcc tgctgaacaa tatgcgctag atacaggaaa cgatccagct
     2821 gagtttaccc aaaaaaatat cgaaacattc cgacgccaaa tcaattcttt aggatttagt
     2881 tatgactgga atcgcgaagt gaatacaact gatccggagt attataaatg gacacaatgg
     2941 atctttacga agctttatga aaaaggtcta gcttatgaag cagaagtagc ggtaaactgg
     3001 gtacctgaat taggaacagt tatttcaaat gaagaagtga tcgatgggaa aagtgaacgt
     3061 ggtggctatg atgtagtcag aaaaccaatg cgccaatgga tgctgaaaat cactgcttat
     3121 gctgatcgct tattggatga tctagaatta gttgattggc cagaaagcat taaagaaatg
     3181 caaagaaatt ggatcggtcg ttcagtagga gcaaatattg aatttaaagt ggctggtacg
     3241 gataaatcct ataccgtatt tacgacacga ccagatactc tattcggtgc aacttactcg
     3301 gttcttgcac ctgaactaga tcttgttaga gaaattacga cccctgaaca aaaagctgct
     3361 gtagaagctt atatcgaaga gacagctaaa aaatcagatc taaagagaac ggatcttgca
     3421 aaagaaaaaa caggagtatt tactggtgct tatgcaatca atccagttaa tggcaaggaa
     3481 atcccaatct ggatcgccga ttatgtatta tcttcatacg gcacaggagc catcatggct
     3541 gttccagcac atgatgaacg tgactttgaa ttcgctcaaa ctttcggact agaaattctt
     3601 ccagttatcg aaggtggaga tgttgaaaaa gctgcttaca cagaagatgg tacgcacatc
     3661 aactctgaat ttctagacgg catgaacaaa caagaagcaa tcgataaaat gaatgaatgg
     3721 ctagaagaaa atggcgtagg gaaaaaagaa gtcagctatc gcttacgtga ctggctattc
     3781 tcccgtcaac gttactgggg tgaaccaatc ccaatcatcc attgggagga cggaacagtc
     3841 acagctgtcc cagaagaaga gctaccatta cggttgccta agacaaaaaa catcaagcca
     3901 agcggtactg gagaatctcc attagcaaat atcgaggagt gggtcaatgt cgttgatcct
     3961 gtaacaggta aaaaaggccg tcgcgaaacg aatacaatgc cacaatgggc aggaagctca
     4021 tggtattacc tgcgttatat cgaccctcat aacaaaaaag aactagcaga ttatgaaaag
     4081 ttgaagcgtt ggctaccagt tgacatctac attggtggag cagaacatgc ggtacttcat
     4141 ttactatatg cacgattctg gcataaattc ttatacgata tcggtgttgt tcctacaaaa
     4201 gaaccattcc aaaaactata caatcaagga atgatcttag gcgaaaacaa cgaaaaaatg
     4261 tcaaaatctc gtggtaatgt agtcaatcct gatgatgtgg tcgaaactta cggtgcagat
     4321 acactgcgca tgtacgaaat gttcatgggg ccattagatg cttcgattgc ttggagcgaa
     4381 aatggcttag aaggaagccg taaattcttg gatcgtgtat ggcgtttgat cgttgatgag
     4441 aacaataaaa tgcgtgaccg tatcactaca ttgaatgatg gcaagcttga taaagtgtat
     4501 catcaaacag tgaaaaaagt gacagaagac tatgaaaatc ttcatttcaa tacagcaatc
     4561 tcccaattga tggtctttat caatgaagcc tataaagtgg acgctctgcc ttatgaatat
     4621 atcgaaggat ttgttcaact actcgcaccg attgctcctc acatgggtga agaactatgg
     4681 gcaatccttg gaaatgatgg tggtatttct tacgcaccat ggccaacata cgatgaagca
     4741 gcacttgttg aagacgaagt agaagttgtt ttccaagtaa acggtaaagt tcgtgcgaaa
     4801 agcaatgttc cccgagactt aggaaaagat gagctagaaa aagcagcatt agccaatgaa
     4861 atagtgcaag aatatattga aggcaaaaca gtcagaaaag ttattgcggt accaaacaag
     4921 ttagtcaata tcgttgctaa ttaattggct ctttgtcaat aaggactgat gagttgtgca
     4981 aaatcaaatc tgggccagaa tgaacttcat tctgtctcag attttttgtt atttcaaatt
     5041 gattaattga ttgatcaaaa aggttttgat tttcatgttc tcaaacacgt tcaatgtctt
     5101 tttcttctga atggtcttgt aagagatctc tgtaatcatc aaatttttgt ctattaatct
     5161 tagtattttt tgatgtataa actacctgaa attgagagta cataccaaat tcataaattt
     5221 tttattagtg ggactagtga ttgtatggat tttgacgcat aatgaatgaa gtatgagaaa
     5281 aggagagaca aatggatcaa gaactattta ttatcgaagt attatcagct aaacaaaaaa
     5341 ttatcgagaa agcgaaaaaa agagcgaaaa gaataatgtt caagctttta agcttagtat
     5401 ttttctctgg tttgcttttt ttcggagctg tatcgttcct atatcgccat atgagtgcag
     5461 tagtccgttc agctgaaggg atgcaaaaat taaataattt gtatcacttt tcgtggggag
     5521 ttggcggaat aacaattgtt gtgcttattg tttttcttct acttatttgg ctccgttacc
     5581 atcaattgtc gcgtcatttc gatcaatggc ttagtcaaag agcacaacag tttttacata
     5641 ataaaccaac ttctcaagat gatgactatc tttattttga gaagacttcg acgagtcaag
     5701 cttttcggat acaaaaaagt gattgccagt tattgctgag atcacttgat gatttaccta
     5761 tctacttagg cgaaagcaaa tcaaaattcg gaatgaacac attccatatt tttttactag
     5821 aaaacgaacc cagatgggaa aaaagccagt cttcgcctgg tggatacctg acaactaaga
     5881 tgagtacatt agtcactggt tttttcctca ttcttttagc tggaacaatc tattatggga
     5941 gatacactca gccaaaaatg tcttctcata taaatgctgc ttctcacatg acaatgaact
     6001 caactaacga tacgcatata caaaagacaa atgaagggtt gttagctgat cctaagcaaa
     6061 aggtaaattt gacaacacaa aagataaatg atctcaaact tgatacagag acgcactctc
     6121 tctatatgac aacagatagt ggtgctaatt gggtatttgt gccgattgat tcgcaatggt
     6181 tgcgttttgg cgaatacacc ctgacaacgg ggctgattcc tgaaggttat tggatggaca
     6241 acacatatga tatttccgcc gatttctcat ggtttatcta ttcttctggg gatgatgtac
     6301 gtctgctgac ttcaagagat aatggtaaaa catggcaaac gtctattatc acccaatcag
     6361 ttcagccgat acgttatcga aaagcggtat tttatggcaa cgatggtcta tttatctgta
     6421 catcagccag tgggatgtct gcagaaactt tgtacattta cactactatg gatggcggaa
     6481 tcacttggat aaaaagtgga agtacaatga tcaatcagcc agtacaaaat gtcagcttcg
     6541 ttacaacttc tttaggtttt gtttcgacac gtgaaagttt gttttataca aacaacggag
     6601 gacgctcatt caaagaatca gttctgtcga ttccggaaaa ctatcagaca ggggggatag
     6661 atctttttca atcaccaaat gaagttactc aaataagcgc gaatacgtta gagacaaaat
     6721 tttatttgtt gaaaaaagga ccaatcgatg cagggaaaat gtttgcctgc ctcttccgtt
     6781 caactgataa cggggaaaca tggcaattcg ttcagcaact atcgcaagta gaacaaggag
     6841 attaaagaag agagattgtg agcctgacgt tcagcaccga gtcacaagaa acaaattcaa
     6901 aaaacagctt ctcatatttg ttgaatagtg taactcgtga ttcggagttg acaagagcaa
     6961 caagaagcga ttttgaagta tagcttctcg tattttacaa gatcgtgtct tattgccgat
     7021 aggttgtctc ttgaacaccg tttattcgta aaaagggatt gtgacaagtc ttttgtcact
     7081 acttaccgaa cgaaactgaa ggagctatcc aaaaagtatc attagtatta ttctttgtgt
     7141 ttctagtggc accactagta catttaaaaa agctatcgat catggactaa tcatacatga
     7201 tcgatagctt tttagataat gtttgttacg ctatctgaaa ttgactatta taaagactgg
     7261 cataaagcgt tggtgattgg agtagttcag aatgtgttcc tttttcgacg atatcgccat
     7321 tcttcattac aaggatcatg tctgcatttt caatcgttga caaacggtgt gcaatcacaa
     7381 aacttgtcct atcttttgtc acagcttcca ttgcttgctg aatcaatgct tctgttcgtg
     7441 tatctacgct agaagttgct tcatctaaaa taacgacagg aggattcgct agaatgactc
     7501 gagcaattgt caataattgt tgctgtcctt gagataacgt gccgttttcg ctagaaatga
     7561 tcgtatcata tccgtctggc agtgttcgaa taaagtgatc acattgtgcg atttcagccg
     7621 cttcgataat ttgttctctt gtggcatcca ttttcccgta ggcgatatta tcagctaccg
     7681 ttccttcaaa cagccaggta ttttgtaaaa ccatcccaaa agaacgacgt agttctccac
     7741 gtgaaagctt ggtaatatca agaccgtcga attcgatctt tcctttattc agttcataaa
     7801 aacgcatcag cagattgact aaagtcgttt ttcctgcacc agttggacca acgatcgcta
     7861 ccgtttgttt tggctttact gaaaaatcga cttgattcat caatatttta tctgatgtgt
     7921 aaccaaactg tacatttttg aaagctattt ttcctttcgg ttgttctaaa agtcttgcat
     7981 ctggaaggtc tggaatttca tctgtttcat ctaatatctc aaatatccta tcgattgctg
     8041 ccattgcgga ttggatcgag ttgatcacat aagaagcggt agaaattggt tcagaaatct
     8101 gattgatgta ctgaagataa gcttgtaaga agccgatcgt cacaccacct gatagaacaa
     8161 gcatagcacc gaataccgca cttatgatga aggcgagttg attaatgaaa cgaatggccg
     8221 gatagatagc acagttcata aattgagcac gtttaaaagc gttgtattgc tttttattta
     8281 cttcgtgtat agcagcttct gcatgagctt gctgattgaa tgttttgacc actagattac
     8341 cagctaagat ttcttccatt cgattattta aaacacccag ttcatactga ttctgttcag
     8401 aaatctgttt gtttttgttc gcaatcttag ttgtaacaat tgaactgcca acgatcagca
     8461 ataagacgag caaagtaagc ttgctttcga tataaaacag catcagacca gcagcagcta
     8521 ttgttacgac agaagtaaaa aattgattga tacctgtcaa taacacttga gaaagttgat
     8581 tgagaccggt agttgtccga ctaatgatgt cacctacttg atgattgtca aaaaacgaca
     8641 tcggtaatgt tttgaatttt tttgtgattt cttttcgcac cctcaatgtg atcttttcgc
     8701 ttaacgctgc cattgtccgt tcctgaataa atgaagtgat gctgcttaag actgaacagc
     8761 cgatcagcaa taagacaggg aacaacaatg cttgtttgat atcactgaaa gaaagctgtc
     8821 caatacctgt ttttcttatt tctgataaga gatcatcgat cccgattccc atgatcaagg
     8881 gcatggctac gactaatagg ttcccgaata aactgcatag aaacaatccg taaaaatatt
     8941 tatgttcagg tttgatcatt ttccaaaaac gtatcaaagc acgcgtgttc aacgtttttt
     9001 tcttttccat cgtatccctt ctttctatat tccttgtgat tttgcaaact cctgataagt
     9061 ttggcaatta gacagtagtt cttcatgagt accttgtcca acgatttttc cttcatctaa
     9121 aacaatgatg ttatctgctt gttggattgt actgaggcgt tgagcaacaa tcacgagagt
     9181 tttctccgac atgtgtttat gaagggctgc tcttaatgct gcatctgttt tatagtctaa
     9241 agcggagaaa ctatcatcga atacatagat ctctgctggt ttgatcaacg ctcgagcaat
     9301 acatatccgc tgtttctggc cccctgaata attagaaccg ccttgagcaa caaagctgtc
     9361 caatccgtca ggaagggtgt ccactagttc ttctaattga gcgatcttga tcgctttaga
     9421 caactcttct gttgaggcat attcatttcc catctgcaag ttgcttaaaa ttgtcccgct
     9481 gaataagaaa gccttctgag ggacataact gatgtgggag cggatctgtt cttgagtcat
     9541 ttgagtaaga ggaataccac caagggttac ttttcctatc gtagggtcac taagtctcaa
     9601 gagaagtttc gccaaactac tttttccaga tccggtcccg ccaacgatcg ctgttgtttt
     9661 tcctttaggt attgtgaaat caatatcttc tagaactgga tcagagattt cagtatattt
     9721 aaatgtgaca tgttcaaaag cagctgcatt tgtctgatca aaggttggtt cagctactgt
     9781 tttgatcgtc tcttcctcaa tttcgatctc tgcttgcagt atttcttcta tccgtcgtaa
     9841 tgaggcaaac gatttaggga taatgaccaa taccatagct gcgatcatca agtaagcaag
     9901 tgtcagcata gagtattcga ctacagcagt cacgacaccg atttgtaatg ctccttctgc
     9961 tacaagattt ccgccaaacc acaaaataga agcataaacg attcccatga gcaaaaaagc
    10021 aattggagtg acccaagcaa atattcggtt cactttgatc atttgattag catagtcagc
    10081 aaagctctta ttcgttcgct tttcttcata tgattgatta tcaaaggcac ggatcatatt
    10141 aatcccagta aagaattctc tcaatgtgat ggtgatccga tccatttttg gctggatact
    10201 caaagataaa ggtgcccctc gtttcatcag tcgataaaca atccatacat aaaaaacaac
    10261 agagatcaat gtaacccatg ttagtaatag agaataacga aacatcatga gaatggtgaa
    10321 aacgcaaata accggtgctg gtaagatcat ttggcaaaat aaaacaatca tttgctggac
    10381 attatcgaca tcattcgtca ttcttgtcaa aagagatcca gaactaaaac gactgatatc
    10441 cttcattgac agctgttgga atttacggaa atatttctcc cgcgtttgga atccaaacct
    10501 agcggaagcc aatgctgaaa agtaactaga ggcaactgca gcgattgttc ctagaaacgc
    10561 tacaagcagc atttcaactc caattttttt tataactgag gcttcgccac ttgctatacc
    10621 tgtgtcgatc agctttgcta ctaattttgg tacacctaat gtaccggcga tttctaaaca
    10681 gatcatcaca aaggtaagga gagcgagtcc tttgtttttc tgtaagaatg tttttaagat
    10741 agacatatta ttcctccttt tttcctgata cttttcgatt ctataccttc aagtaacttg
    10801 aaggtcaata gaaaaaagaa taaactgaaa aagaggtgag aaaaaatgaa aaaaatgtat
    10861 acgattggcc aagtatcgga actatttgat ttgccaaaat caacgatccg ctattgggat
    10921 gaacaaggat taatccgttc aagtagacaa gaagagaatg attatcgatt gtttgatatc
    10981 gatgatattt ttatgcttta tgatatcgtt ttttatagaa aattagatat accgatcaaa
    11041 cagatgaaga atttatatgg aaaaacattg acggagttat atgagacact tgatgaaaca
    11101 gaaagaagaa tccataaaga actggtggtg atgaaacaaa aacaaaaaga gataagggag
    11161 caaaaaaaac agttgaaatt gatgatcgat acaaacgagg aagaatttcc tgtagaagag
    11221 atcccttttg actgtatgat ctcaacggag tttgaagata ttgtggaaat caaaaaattt
    11281 ctacctaact attcaagctt tgggatgatg agtatgccag cttccagttc acagacgatg
    11341 tatggatttt ttatcgaccc tagtgaagta catttattta ctcaagacgt gatttgggag
    11401 aaaaaagata cggctgttta tcgtcgattc ttattgaaat ccgagatgaa ccatgcggaa
    11461 cgtaataata ttttagaaat cagagaaaga atgtatgaaa aaggatggaa aacaggagaa
    11521 gtgatcggcc agtatctatt gacgaatacg gatgaaaaca atatcagaac tgaatattat
    11581 catgcatgga tagaaatgaa aaaataggaa atatgtgttt aagataactc gaaaatgatg
    11641 gttgttttag ttataaagaa aaattttctg gttcgattac gggtttaatg tgggggatta
    11701 ttagtcgtgg atagatagaa ttttagtttt ttagaggaaa tatgtcttta aaaatggaat
    11761 ggttgcaatt cttagcgata tatacgatgt aattgtcctc ttaaaaaagt atggaaaaat
    11821 aattgtattg ggtgttatgt ataattcatg gtgaactgag actaatgaga agaaaaattc
    11881 atattgttat gagtgagatt gtatcagatg agaatgataa attaaatagg tgcataaaag
    11941 cattacactt aaaatacatt gttgcttaca tagtcaagtc cataatcct
//
LOCUS       DASNNU010000080        11858 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.080, whole genome shotgun sequence.
ACCESSION   DASNNU010000080 DASNNU010000000
VERSION     DASNNU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.080"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(218..2293)
                     /locus_tag="U8116_002133"
     CDS             complement(218..2293)
                     /locus_tag="U8116_002133"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEN8435097.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2295..3212)
                     /gene="glyQ"
                     /locus_tag="U8116_002134"
     CDS             complement(2295..3212)
                     /gene="glyQ"
                     /locus_tag="U8116_002134"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEN8435098.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3598..4416)
                     /gene="recO"
                     /locus_tag="U8116_002135"
     CDS             complement(3598..4416)
                     /gene="recO"
                     /locus_tag="U8116_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEN8435099.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4559..5458)
                     /gene="era"
                     /locus_tag="U8116_002136"
     CDS             complement(4559..5458)
                     /gene="era"
                     /locus_tag="U8116_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEN8435100.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5473..5868)
                     /locus_tag="U8116_002137"
     CDS             complement(5473..5868)
                     /locus_tag="U8116_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEN8435101.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5852..6328)
                     /gene="ybeY"
                     /locus_tag="U8116_002138"
     CDS             complement(5852..6328)
                     /gene="ybeY"
                     /locus_tag="U8116_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEN8435102.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6357..8549)
                     /locus_tag="U8116_002139"
     CDS             complement(6357..8549)
                     /locus_tag="U8116_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEN8435103.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8573..9544)
                     /locus_tag="U8116_002140"
     CDS             complement(8573..9544)
                     /locus_tag="U8116_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEN8435104.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10249..11628
                     /gene="brnQ"
                     /locus_tag="U8116_002141"
     CDS             10249..11628
                     /gene="brnQ"
                     /locus_tag="U8116_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN8435105.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         3231 a         2523 c         2164 g         3940 t
ORIGIN      
        1 tagggaaatt cctaatttaa aagtaaataa aacagtaaca agattataat taatcgtttt
       61 catttttatt tcctcacttt tcatgagcgt tttgaaaatt cgaacaaaga ataaaataac
      121 aaaaattatt aaaatagaat taataacagc tatttataac aaaaaaaaca gagacacagc
      181 acgtttcgtg catgctctct gtttttcaaa caaaagacta ttttgtgatg atttgatcca
      241 agcttgctaa tgacaaagcc atggcagcta tttgacttag ttgtgttagt cggttcttac
      301 gtactgctgg ttcatcagcc atgaccatcg tttgatcaaa ataattttcg atcagtggac
      361 gaagagcgac aagctggtcg tagttttcgt ggatcgtttg gtcagcaaaa cttgctttca
      421 ttgcttctac tgctttgtgt aattctttct ctgcgtcgtt ttcgaacagc tcagggtcta
      481 cttctgattg tttttcaagc tgcgcttttt tcgctaagtt cacgacacgt gtcaaagctt
      541 ccattgaagg tttgaagtct tcatctgcaa gatgttgttt gaacaattgt gctgctgcaa
      601 atacttttgt cagatctttt tgttccgcat tcaaaacagc atcgatgaca tcatgacgaa
      661 tattcttcgt tgtcaataat tggcgcagac ggcctttgat aaagtcgatc acttgttgtt
      721 gcccttctga taaaccaatg ccgaaacgat ccgtatcttt actgataaca tcgctaatcg
      781 tttcttgcag aacagacaaa ggaaaggccc attttttcga ttcgatgatc cgaacgacac
      841 cgtatgcttg gcgacgtaag gcataaggat cgtttgaccc gcttggaatc atcccaactg
      901 caaagaaaga aaagatgcta tctaatttat ctgcaatcgc taaaacagaa cctactgtag
      961 actgaggtag ctctccttca ctggatatag gcatgtaatg ctcacgaatc gctgtcgcta
     1021 cagcaggttt ctcgcctttc agaagagcat atttttcgcc catgatccct tgtagttcag
     1081 gaaattcccc taccatgttg gttactagat caaatttata gatttcagca gctcgttgca
     1141 aatccatcaa ttcttcctca gatagtccta cttgttcgcc aatgatacgg gcaatgactt
     1201 gtacccgttg cattttctca tagatactac cgattttttc atggaaagta acagttttta
     1261 gtttttcaac gtattggtcg attgaagctt tcttgtcttc attataaaag aattccgcat
     1321 cttctaaacg agccgtcaat actttttgat tgcctcgaac cacatcttcc aattttaccc
     1381 gatcaccatt acgagcagaa ataaaatgag gaagcagcat accttgatca ttccgtacct
     1441 cgaaataacg ttgatgttct ttcatagaag tgactagtac ttcttcagga acagaaagat
     1501 atttttcttc gaagtcgcca gaaaagacag ttggatattc gactaaattg ttgacttctt
     1561 ctagtaaatc ttcgtctaga tcaataaccc agtgattttt ttctgctaat tcgtttgctt
     1621 gtgtaacgat catttcttgt cttttttgcg gagacgcaat gacgaattgt tcattcaatt
     1681 tttcttcata ttcttttgca tgcgcaaatg tcacatcttc gcctaagaaa cgatgacctc
     1741 gagaggctct gcctgttttg acatccaaga tttcgaatgg gattacttcg tcatctaaca
     1801 atgccacgat ccagtgaata gggcggatat attcaaaatc gtattctgcc caatgcatcg
     1861 ttacagggaa agtcaaactt ttgatcaccc ctgctaagcc tgtcaatact tcttttgctt
     1921 ccttaccatg agtgtatttc gttacataga catattccac accttttaat tctctaaaag
     1981 tgatagcgtc tgtcgtcaga ccttgtccgc ggacaaatcc ttccgctgct ttcgtccagt
     2041 ttccatctgc gtccaaagca atttttttgg ctggtccttt gacttcttct tcactgtccg
     2101 cttgtcgttc tggaatattt gttactttga ttgctaaacg gcgcggtgtg gaaaatgttt
     2161 cgatcgtatc ataagtcaat tggttttcat ctaaaaattt tgctgttttt tcttctaatt
     2221 gtttcatgct tggtgtgaca acatgggcag gcatttcttc cagcccgatt tcaagtaata
     2281 agtcttttgc catcttattc agcctccttc acggtttctg gatttgctgt gtttttattc
     2341 aacaaaggga atcctaattt ttcgcgttct gccacaaaga ttttggctac agcacgagcc
     2401 atgttgcgga tgcgagaaag ataaccggca cgctctgtta cggatactgc accgcgggcg
     2461 tctaataagt tgaatgtatg gctacatttc aagatataat catacgccgg atgaacaaga
     2521 ttttcatcga tacagcgttt agcttctttt tcaaaacgat caaagttgct tagtagcaag
     2581 tcttggtcgc ttacttcaaa agaatatttg gaatgttcat attctggttg tttgaagatc
     2641 tcgccatatt tcacgccatc agcccattct aaatcataga cactttcaac ttcttggata
     2701 taagaagcaa gacgttctaa tccataagtg atctcagaag ttactggttt acaaggcagt
     2761 ccaccaactt gctggaagta cgtgaattgt gtgatttcca ttccgtcaag ccatacttcc
     2821 caacccacac cggcacagcc catggatggg ttctcccagt tgtcttcaac aaaacggatg
     2881 tcgtgttcta atggatcgat tcctaaaagg cgcaagcttt ctaaataaag ctcttgaata
     2941 ttctcaggtg aaggtttcat caccacttgg aattgatgat gctgatacaa acggtttggg
     3001 ttttcaccat aacgtccatc agctggtcgg cgagatggct ctacgtaagc agcattccat
     3061 ggttctggtc cgattgcccg taaaaaggta tatgggctca tcgtacctgc tcctttttct
     3121 gtgtcataag cttgcatcag catgcagccg tttgatgacc aaaatttttg taatgttaaa
     3181 atcatatctt ggacggtcaa ttttttactc atctgttttg cccctttcaa ttttaaagcg
     3241 ttgctgtcaa ataagaatac aggaaagaaa acgacaaaaa atccctatgt ctgattacag
     3301 acatagggac gaataattcg cggttccacc ctacttccga ataaagattc ggcagcttca
     3361 tttatcgtac tcgcaagtgc ctcttcaaaa atcgtttggg tccggctccc actctcccga
     3421 actcgctaaa ccacgtgttt tttactgatc ttggtcaatg tacgtattca ttttgcagtt
     3481 ttcttgtttc tataatagat cctatcactg aacgatctat ttaatctatg gttaataata
     3541 gacggaactc cctgtgtttg tcaaatggtt ttgatttttt gttgtatgtt ttatttacta
     3601 ttcttctttt tcaggtgtct ctttttcttt ttcagacatg tctttctctg acgtctttaa
     3661 tacgtgtgcc caagacttca tttgattgat gaactttttg cttttcaaat ggataccgac
     3721 atattcatca tacagctggt cgatgagttc acggattgct tgtttggttt cttctttcag
     3781 atggattcca ctaattttat cataagaaat agctgaaaac atccgaacga aataaacagc
     3841 tcgaggatct gcatgataac ggtgatcgtc catcgaccaa tgtctttcgc atatcacacc
     3901 accatatttg gaagaatagt caaattttcc ttttgtttcc ccgcatacga cgcagtgtgt
     3961 ccagattggt gcaattccga agcgttgcag tatttggact tcaaaaatat tcgtgatgat
     4021 ttctgcatcg tctccttggt ccatccgttg cagtgcttgt tgcgtaaact ggaacaaatg
     4081 cgggtcgtac tgatgatctt cgattgccgc atctactaga ttcaataaat atgtgccata
     4141 gccgctcaaa aagatgtctt cttggatgtg tcgaaacgca tggacatctt tgacactgct
     4201 taaaaatgac aaaccttcac ttcgaaaatt cccgatataa actgcttcag taaaagggag
     4261 caaagccgga gctaagggct gattttgccg atggatctct ttgatataaa acatcatttt
     4321 gccagaagat tctgtaaaga ttttgactaa tttatctttt tctttgtaat tacggctaga
     4381 taaaatcaag ccttttgatt ctgcaagctg gctcatattc tccctcctaa aaaataaatc
     4441 tggaaaagca gattctgccg atccagatca tttatgttgc gatcttccgc agcttttctc
     4501 aacacgcgga tttttattca gtttatttct atacctaatt tacaagaagc tcagaatatc
     4561 agtattcgtc ttttcggtag ccgaagtctt gcagatagac ttttttgtct cgccagtctt
     4621 tttgaacttt gacccaaagt tctagaaaga ctttgtctcc taaaagattt tcgatatctt
     4681 ttcttgcttt cgttcctact tgcttcagca tttttccgcc tttgccgata atgatccctt
     4741 tttggctatc tctttcgaca ataatcgtag cctggatatg gattttatca ttctcgtttc
     4801 gcttcatcga atcaacaacg actgcgacag aatgaggcac ttcgtctcgt gttaaaaaca
     4861 agactttctc acggatcaat tcagaaacga tgaagtattc aggatgatca gtcacttgat
     4921 catctggaaa atattgtggg ccctctggca tttcactgac taatacatcc atcaaacgtt
     4981 cgacgttgtt tccttctgta gcagagattg gaatcacttg tgcaaaaggc atctgcgttg
     5041 aataatcttc gatgattccc aacaactcgt ctggatggat tttgtcgatt ttattgataa
     5101 tcagataaac aggagaatgc acgtttttta gacgttcaat aataaaatcg tctccccgac
     5161 cgcgtttttg atcagcacta atcataaata agaccgcgtc cacttctttc aacgcactgt
     5221 aagctgcttc taccataaaa tctcctaaac gatgctttgg tttatggatt cctggtgtat
     5281 caataaaaat caactgagct tctggtgtag tgtatactcc ttggatttta tttctagttg
     5341 tctgtgcctt gtcactcatg atggcaattt tttgacccac gattcgattc aataatgtgg
     5401 atttccctac atttgggcgg ccgacgattg cgacaaaccc tgatttatgt tctggcatat
     5461 atgttctcct ttttaattaa acaacaaatg atatatttta gggacaaata aaatcaaccc
     5521 aacgatgacg gtaaaacaag cggtcactaa aactgctcca gctgccatat ctttgatttt
     5581 tttcccaatc ggatgaaaat gaaaatccgt aaacatgtcc acgacatttt caaaaacagt
     5641 attgatgatc tctactatcc atacgagaaa aacagctaaa agcagccata accattctat
     5701 ccgatcaagt tggaaaatca cgcccataaa caaagcgagg attccgaacg caacatgttt
     5761 cctcatgttg cgttcttcct caaaaacagt tttgactcct tggacggcaa attccacgga
     5821 catcaggaaa tgtttgtttt tctctacgtt tttatctttt aagtccatag gcatctaaga
     5881 tctctttctg taaaccaaac atttctttct catcctctgg tgtcatatgg tcatacccgt
     5941 tgatatgcaa gaatccatga accgctaaaa agccaagttc ccgatcatac gaatggccgt
     6001 aagcttctgc ttgctctgca gcacgttcag tagagatcat gatgtctccc agtacacgag
     6061 gcaatggatt ctctccatct tcaaaaatca ctgggatttc gtcttcgccc tcttcttcca
     6121 aggcaaaact gatgacgtct gtcggtgcat cttttccacg gtaatcacga ttgattactt
     6181 ggattgctgc attgtccatg aaagtaacag acatttctgt ttcttctggc aactctaaat
     6241 atgatgctgc aaattgcagc aagtcgtcga tttctttcat tttctcttct gagacttggt
     6301 ttgtttcatc catgaaggta atatccatta tacatcgctt ctttctattc ttcttgctat
     6361 tcttcttgtt cttcttttat cttctcaact tccgatttca ttttaggata tttgatccga
     6421 gaatggaagg tactgcttaa accgctgatc aatgattttt ctactttacg gatttctttt
     6481 aacgtcaatc cactgtcgtc caattgtcca tcggaaatac gctcttcaat aagattatgg
     6541 acaaaacttt cgattttttc gctcgttgga tggtccattg cccgtactgc cgcttcacaa
     6601 ctgtcagcta tattgacgat tcctgcttca cggctttgag gttttggtcc aggataacgg
     6661 aattgctctt ccgtaacttc cgggttgcgt tcctttgcct taacgtagaa ataacgcatc
     6721 agcgtggttc catgatgctg tctacaaata tcgatcacca tttgcggcat attataatct
     6781 tccaatattt tggcaccatc tgtcacatgt ccaaagataa tatgtttact gtcttctggc
     6841 aataagaaat tgtgcggatt ttccgctcct gaaggcaagt tctcaacgaa aaagctggcg
     6901 tgtttgatct tgccgatatc atgataatag caagctactc gagttagtaa agaacgtcca
     6961 ccgatttctg ctacagcatt agcacttaga ttggctacca tcatgctatg gtgatacgta
     7021 cctggtgctt cttccagcaa ctgtttcaat aaaggatgat tgggattgct gagttcattt
     7081 aatacgatca cactatcatc attgaccatc aattcaatat aggggtgcag acctatcgtc
     7141 agcaagaatg aaaacagtgc acctgtgaat ccgcagacta gcgttaacca agtcgttgca
     7201 tccccaaagc tcattccttg gtagataatc aaaatgacat ccatcatcac aggaaaaata
     7261 atgacccaca tagcagcgga taacccttgt tctgagatcc gtttgcgtct gacgatcgtt
     7321 gccatgagac cagaaaatac ataggacatc aaaataactg tcagaaagtt cgtgccaatc
     7381 gaatcataaa aaatgaaaag tgcggatact gcttgaaaaa taccagccat gatccctgct
     7441 cttcgattca agaaaaagct taaaacaagt ggagcaaaag ccgcggggta aaacagcgga
     7501 atataagcag cttgttctgt ttggaagagt tggaaaaatt tcattagtac gacgcttact
     7561 gacattgccg tcacgtagaa aagtacgtat tttgttcttt ctcctgcatt ttggaattga
     7621 agtgaattat aaatcagtac accgatttga agcaaaacag ctaaaaccat ggcaatgagt
     7681 gggaaaatag aagtcgtttg attcgtcaat cctagtaatt ccaatttttt catcgctgaa
     7741 gcatcaattt gatttccttc gcgaacaata atttctcctt ggaagatcat cactggttgt
     7801 acagcttctc gtgcagattg ttttaattct tctgtctttt tctcattcaa tacatcatta
     7861 actacgatgc cttgatccac taagtagcga atcatttgtt gctgctctgt tgtgacattc
     7921 aaatattgaa tctgatcttc tgcttcttgt ttaaaagctt ccaattcgct ttcacgaact
     7981 tgtttactca tctgttcgtc aatgagcttc aaactttcat ccctaactgt gtgaatttcc
     8041 gtttcagtca tttcaaatat cgtcgaataa aaattgttcg gcagtttttg gtaaaacgca
     8101 acgttttctg cattcacgtt ttcaaaattt ttcttcagtg tagcaatccg ctcttctgta
     8161 gtaggtttcg ggatattttc atcttccttc gccttatcta ccttgccatt ataagatttg
     8221 ttgatggtat cattcgtctt atcgatcaat tcgaataatt gcttgatccg attgttctgg
     8281 tcatctgcca gatctttttg ataagtatat tctggagtca ctgcttctgc tgccagtttt
     8341 cttctttgtt cggtttcttc cgtgttctca atcgttttat tcgcacggat actctcttct
     8401 gctacttggc cttctctgaa atcaatggcc ttttgtttga cactactaaa tgtaacgccg
     8461 cagacaatga taaaaaacaa gaacagaagg aaaggcaatg ctgcctttcc gagtttttct
     8521 tgtagtttct gcagtgtttt attcaacatg tgcttatcct cctaagcaaa gcttattctt
     8581 gatgaagatc cgccttttca taagctttga tgatttcggc aactacaggg tgccgaacaa
     8641 cgtcacttgc ttcaaaatta acaaaatcga tttttttgat ttgctttaat gttcgttctg
     8701 catgaacaag accacttgtc gtcccacgag gcaagtctat ctgacttgta tccccattga
     8761 caatcatttt tgattgataa ccgagacggg tcaagaacat tttcatctga gcgactgtcg
     8821 tgttttgtgc ttcgtccaag ataacgaatg catcatccaa tgtccgcccc cgcatgtaag
     8881 ctaaaggtgc gatttcgatc acgcctcgtt ccatcaatcg gttcgtatga tccaatccaa
     8941 aaatttggta taaagcatca taaacaggcc gcagataagg atccactttt tctttcagat
     9001 ccccgggtag aaagcctagg ttttcccctg cctccacagc gggccgcgtc aaaatgattt
     9061 tttgcacttc gccttttttt agggcagcaa ttgctaatac tacggcaaga aacgttttcc
     9121 ctgttccggc tggccctaca ccaaatacga tgtcatgttt ggctaccgat tcaacgtatt
     9181 tcttttgacc gctgtttttt acccgtatag gttttccatt acggtctttg acgatctctt
     9241 cctcatacat ctcaacaaaa taatctaatg tacctttttg ccccattttc aatgccgtca
     9301 caacatctgg cgtacttacg tggattcctc gtttgatcaa ttcctgtaat gtccgaatga
     9361 ctgaggcagc caaggaaatc tgggattgct caccaatgat ctgaatgatt tctccacgtg
     9421 tatggatggt tgtttccgtt gtttcctcga tcagttttat atgcttgtcg tgtgcaccta
     9481 aaagcatgct gacatcatca gcagctgtca atctaatctc taaagaacta gactcttccg
     9541 tcaaaaatag acatcccctt tttttcgatt cattctttcc tattttacca tactcagaag
     9601 aaactatata gcaaataaga aaaggttgta aaagagtcgc ttaaccatac gtaccaagaa
     9661 acgttaaaaa atagccgttt ccattcgcta aaggtctcgt tttgataaaa agaagctgag
     9721 aatgtaagtt atatgaaagt ggagaacaaa atatttcgtt tcattcaaca agtattctct
     9781 aggtattttt cctgtttcaa aaaaagaaaa caaaatagtt aaaatattct tgcatttcaa
     9841 aaaaaacagg tgtattatta cgtcaacaaa taaacataca gcaaagcgtc gaggaaaaca
     9901 gtaggctatt caacgattta ttcccagtga gtcatgtaag tgagaaatga caataaatcg
     9961 aatagacgaa catcatttcc gagtcaatgt cgaaaacgta gatcattgtg gctgcctcca
    10021 ttatcaggct agcggataac tttatcaaaa agcgtccgtg aatgaagaag cttttttaag
    10081 cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt cccaagtgga aaagggcttt
    10141 tttttatttt aaggtggtgg ttttcatgga atctgatatt agtggcatta ctaattgctg
    10201 caacgtttat atcgtcaatc aaaatgataa ttattggagg aaaaatttat ggaagaaaca
    10261 aaaacttatt catggaaaca actattgatg gtcagttcac ttatcttcgg gatgttcttc
    10321 gggtcaggta atctaatctt tcctgtccac ttaggtcaaa tggctggcgc aaactgggtc
    10381 agtgctggcg taggatttgc tatctcaggt gctcttttcc cattactagc tatcttagct
    10441 gtagtagtga caaaaagtga tggtctgtat gacttagcta aacctgtcgg aaaatggtat
    10501 gctgccttgt tcttaatcct tgttcatttg acgatcggac catttttcgg tacaccacgg
    10561 acagctgcta ctgcttttga aatggctgct aaaccatttt tgcctgaaaa atataattca
    10621 ttaggtatgt ttttattctc tgctcttttc tttttacttg cttatttcct tactgtccat
    10681 ccaacacggt tggtcaaata cgtcggaaag tatctaaact ccttgtttct tgcattatta
    10741 gcaatcattt ttgtcgtttc tttattccat ccaatgggaa atctaaatca atctgctgca
    10801 gatacttatc aaacaaacgc tttaacaaat ggtgtcctag aaggttataa cacggttgat
    10861 gcagtagctt tgcttgcctt aagtgtcact tttgtccatg cagtccgtga ccttggtttc
    10921 aaagataaaa aagtcacaga attaacggca aaagctggta gcctgagcgt tgcgctagaa
    10981 gtcatgatct attttggact tgttcttctc ggtgcgatga gtttgaataa gatgtcttta
    11041 tcagaaaatg gcggaactgc actttctcag atcactaatt tttatttagg taacttcggc
    11101 gctagcttct taggagtgat ggtcacatta ggtgtcttca ctaccgctat gggactcgtt
    11161 gtttcttttg cgcaagattt ccataaactg ttccctaaag tcagttatat gacatggctg
    11221 cggttaacga ctttcgtctc ttttgtcgtt gcaaatgctg gtttagacaa tatcatccaa
    11281 tggtctttac cagtattgat gcttttgtat ccattgtcgc ttgcgttgat cttgctttcc
    11341 ttaacagcaa aatttttcca aaagacacca tttgtctatc aagtaacgat gttgtttgct
    11401 gctgttcctg ccgtattgga tatgctggca aattcgcctg ctttagtgag tcaacaaaga
    11461 gttgtagcaa gcatgctcga attttatcat catcacgttc cttttgctgc attaggactt
    11521 ggctggatgg tcccaacgtt acttggttac gctggcagcc tactgtttta ttacgcctat
    11581 cgcctatcag ggtataaaca agaagcaaat gaactaccag aagaataacc aaatcaaaca
    11641 caatttaaca taagaaatat taaacaggaa aaaaccagct agatgaaaga atcctattct
    11701 ttcgtctagc tattttttag ttctcttttt tacgcgcaca aaaaaactgc ggacaatgat
    11761 tgtccgcagt ttaaatgggc tctataatat ctagtgttgg tgacgccttc tgttgccaat
    11821 actttttctt ttttctaata aacgcaaaca gacacctt
//
LOCUS       DASNNU010000081        11391 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.081, whole genome shotgun sequence.
ACCESSION   DASNNU010000081 DASNNU010000000
VERSION     DASNNU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.081"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(56..2758)
                     /locus_tag="U8116_002142"
     CDS             complement(56..2758)
                     /locus_tag="U8116_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN8435106.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(2771..4060)
                     /locus_tag="U8116_002143"
     CDS             complement(2771..4060)
                     /locus_tag="U8116_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEN8435107.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            4210..5256
                     /locus_tag="U8116_002144"
     CDS             4210..5256
                     /locus_tag="U8116_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN8435108.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            5258..7411
                     /locus_tag="U8116_002145"
     CDS             5258..7411
                     /locus_tag="U8116_002145"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEN8435109.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(7884..9335)
                     /locus_tag="U8116_002146"
     CDS             complement(7884..9335)
                     /locus_tag="U8116_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN8435110.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(9332..11056)
                     /locus_tag="U8116_002147"
     CDS             complement(9332..11056)
                     /locus_tag="U8116_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN8435111.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(11049..>11391)
                     /locus_tag="U8116_002148"
     CDS             complement(11049..>11391)
                     /locus_tag="U8116_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN8435112.1"
                     /translation="YLSVQIKGLIFLAIDFILLFALLCLFATYQYSMILDSEYTISVP
                     NLLKLSFISVFSSFSSFLKIIIGSGIILGVTWQFKGLILFGVIGLLTVWNGTMTTHWR
                     EELDKQLESYE"
BASE COUNT         3197 a         2294 c         2002 g         3898 t
ORIGIN      
        1 tctaaaattt ctccaccatc tgaaaccgca cacacaaatt ttgttccgcc tgcttctatt
       61 ccgccataca ttcgttttcc tcctcgaatc cgattgtccg aatctcataa ggttggatta
      121 ttgggacaac cgcttgcttc gttggttctt caagcagatt caatatgaca gtttttccat
      181 tgtctttagt tatttccaat ttttctaaat gactagacat attgaaacct cttactacta
      241 ctttatcact aaacttgctg cgtttcaaag ctgtaattgc aaacgtttca ctttttatcg
      301 ttaaatattg ttgttttgaa atcaatgttc cttcatgatg tttgatttgt tgagtggaaa
      361 aaggaatctg tgctgcgtat gcatgcaaat aagtagagaa tttttcttct ggtccatgta
      421 gttcaatgct gtaattgaac cgatgctcac ctagacactg tgcttctggt gttggaaaat
      481 atccccaatc gcctagttct cccacactgc gaagtagcgt cactgcgatt tgtccgtctt
      541 ctgttacttc atattcattc aaaccaaagt ttccgactgt tacaccgtac tcttcatcat
      601 gaatattgac aaatgcttgc tgatgttgcg gattcgtggg gttttcccaa cttttagaca
      661 catggtttgg gcgcttcacc acttcaaaaa tactatctgc ttcgtgatgt tccacatgta
      721 actttgtagg aaacaagaca cgtaagcgat gatctttcat ctgattgtcg agagtcgttt
      781 caaagtcgat ttttttacta tcttttctga ttgtcatgat tgtttttatt tgaaggattc
      841 gtttttcttt ggatcgttct gcttttcgat aacgaaattc cacaaccatc tgctgttctt
      901 tttctaaacg ctcgtctgca gaaacaggga tttccatttc ctgtagaata cttatttttg
      961 ctatttccgg tgtatctact aatactttta cttcagagtt ttctttgtta ctggatagga
     1021 ttggctgatc acagaaaggt tgtttaaaga tatactcatt tcctatatcg ccgatatctt
     1081 caaaaatatg cagattattc aactttcgtt tgtttttgcg atcttccatt gtaatggtac
     1141 catttttttc aatcgtcaaa tgtagagggc cgttttcgat gattcgtcca ctctgatgaa
     1201 tcattgtttc cttttcttct gctttcgctt ccccttctac aagcgcgaat gtttcccaag
     1261 aaaatgctgg catgttttct aatggtaaag tcactttgat cattcttgcc atatatggca
     1321 cacggaaacg gtctttcggc aaatcataac caaattggac agtttcttca gataaaatag
     1381 ctggtatagt ggccccactt tttgtttcta catgatactt acgtttaggc aaattttcta
     1441 actcttgata tagttgttca ggtattcctt cagcgaatct ttttctttct aattcgatgg
     1501 ttatttctgc ttcaccagtt ttatccattc ctgcagtatt tacaatgaca aatggaaagc
     1561 tgtcttttgg gaatccggtc gtatcgattg ctgcggtcag ctgttctaag gaatcttgtg
     1621 caagatactt tccaacttca tctgcttttt caaaacgagg tatcatttcc cgatggacag
     1681 aatcgactga acaaccgcaa atactgtcgt gaggatgatt ttgcattaat gtcttccaag
     1741 cataatctaa ttgatcatgg ggatagtttc cactaacttc ataagccatc gtcgcaagtg
     1801 gttctgtaat attttctagc tgtcgctgga ccttggtatt ccattgtttt aagtaaaccc
     1861 gagcagatgc tgtattggct aaagtatacc agccatccgt ttcttgactt gtcaattctc
     1921 cttctactga gcctaagtct tctggcacat ctttttgaac tgcttccaga tagtcagtaa
     1981 aattagaatg aataaactcg taatctggaa acagttcatt tgcgagatgg attgcttttg
     2041 atatatcttt ttgtaccggt tgatgatcaa caccattcat catcaataag tggttcgtag
     2101 aggcatattg ttctgcatcc gctaattttt gcttccaaaa ggcaagagct gcttcttttt
     2161 ctactggaat ctcattcccg ttgctatacc aattggcaaa tagcagacca aagattgctg
     2221 tttgatcagg tcctttccac cacatctctg agtattggga agaatagttt tctgcttcca
     2281 aaacttgatt atcaaagcca attggtttta caccacggcc aaacgctgct gctgaaatac
     2341 cagcttgttt cattaattga ggcgtctgtc ccatatttcc aaatgtatcg ggaaaatagc
     2401 ctaacatgac aggggttccc catttacggc tttcttccat gccaatcagc atatttctca
     2461 cgttcgattc cgagctgata agaaaatcat cttgcaagat ataaaatggt ccaattctca
     2521 attttccttc attgattgct tgttggattg cctgtctttt ttctggcctt acttgaagat
     2581 agtcatctaa aataatcgtt tgcccatcca aatgaaaact gttgaaggac ggatcaactt
     2641 caaatagctc tagtagatcg tctatcaatt caatcagtcg catgtgatgc tgttcgtaag
     2701 ccatgtacca ttcacgatcc cagtggctat gagaaatgat atatactttc tttttcattt
     2761 acgatacctc ctacttttct actctaatat caaagtaatc catgactaat tcgcaaaaca
     2821 tcatatttgc ccaagaaaac cattctctcg tataattttc aggattgttc acatcgaatc
     2881 cttcgtgcat aaggtgtgtt cctgcgtcag ttgctacaag taaattcaat atccgctctt
     2941 tttcagattt gtctgacgtt gtcattcctt ccatagctaa tgcaatcggc caaacgtatt
     3001 tttctggcgt atgggaggat ccaattcctt ttgcgtactt tccttcatag aaatatggat
     3061 tttctttact caacaatgtt cttcttgttt gcagataatc ttcctcttgt tcagaacaat
     3121 atcccaagta aggtgcggcg atcagattgg gcacattact gtcatccatg actgtggcgt
     3181 ttcccattcc atctacttca tatgcataaa tcgtttgccc tttttgatta accgttcgtc
     3241 cgtgattctg aatcccctct tggatctctt tcttcaattt ctgtgtttgc tgtacaattt
     3301 ctttatcatc gagtatcgtt tgaaaaattt cttccacata acctaacacg accaccgcaa
     3361 acatattgga tggtaccaga tatccataac ggcaagtatc atcgctagga cggaaaccag
     3421 accatgtcat tcctgtaaaa gctgtttttg cacctcgccc gtcattgatc agcgtatctt
     3481 cttttcttgt cgtgtctcgt acaaatgtat aaggagagtg ttcatgatct tgctctgttt
     3541 tgaacacgtc caagattttt ttgatacctt caataaaaga ttgattgaat tgatcagttt
     3601 ttcctgtatt tttgtaaaga agataggcca actgtattgg gtagcataaa gaatcaattt
     3661 catattttct ttcccagatc caatcgttca tttttgtatg atctgtttgg tggccagcac
     3721 cgttagcttc ttcgttgaag gcattcgcat atggatcgat attgatataa aaaaattgct
     3781 ttttgactaa cccgctgatc atgtcagcta attcctcatc ttcttttgca atcactaaat
     3841 aaggacgaac ttgtgcagta gagtctctta accacatcgc tggaatgtct cccgtcaata
     3901 aaaaggtggt tccgtcattt tgctttttga ctgttgtcaa cagagtattg gcaaatgctg
     3961 ctgtaaaatt ctttgcccag tccgcatgga tatccccaca tttttgtgtg atatcttcca
     4021 taaatttctg caccgatttt ggtatatctt tataagccac taataaaacc ttctttctat
     4081 ttgatatatc tttaaagatt atacttcata cagccaatta tgtctacgct ttcaaaaaat
     4141 atctttttat ttactttttc atccattatg gtattatttg aatatgatat atcattaaga
     4201 ggtgtttata tgaatcaacc attgtaccag acaatattta atgatttgca aacggctatt
     4261 ttaactggtg aattacctgt agacagccaa ctaccaactg aaaaagaact ttcacgtacc
     4321 tataaagtaa gcagaatcac ttccaaacgt gcattgactg aattagagca attaggactt
     4381 atttatcgcg ttcgcgggaa aggcagtttc gttaaatccg cttctccgga acagattctt
     4441 gcttctccta caaaaacacg acggattctg tttcttcttc catatgtcgc tgatttgtct
     4501 gttggtgatt ttagcaaggg attgaaccct gtgatgcaag ataaacagat tgatgtcatg
     4561 atgacgacgc ttgactttct acaaaataaa acagcaaaag agctaatcaa tgaatttgac
     4621 ggattgattt attatgctct tgatacagaa caacatcttg acttactttt tgagctttca
     4681 ttgaaagaat tccctgtcgt catattagat aaaaaaatct atgagttacc ttttcctacc
     4741 gttctttccg ataatttcca aggcggtgcg cttgctgcct cttatttgat cgaacaggga
     4801 cattcaaaaa tcgcttattt gtttggtacg cagactcatc cacaatccgt tcgacagcgt
     4861 taccttggtt accttgaagc attggacaaa gctggtttga ctttccacac ttcactggat
     4921 gacaaaagcg cgacattaga tgaattgctt tcttatatca ctaagtatga agtaactgct
     4981 tttgtttgtg aaaacgatgt tgtcgctatc cagaccatga atctcttgag acagcatggt
     5041 tatcatatcc ctgaagattt ttcaattatc ggatttgatg atatccaagc agcagctctt
     5101 gttgacccgc cactgacaac catcgcacaa gattttgaac agttaggtaa gcttgcggga
     5161 gatgctttgg tcacttggtt agagacaaaa gatcgaccat ccgatagtaa atttccagtt
     5221 tcattcatca aaagacaatc aacaaaggag aaataaaatg aatatcggtc aaatagatac
     5281 tagacatgca acagcgaatc agccaaattt ctctaatggg aactgccagc cttatactgg
     5341 cgttcctttt gggatgaatt attttgcacc gcaaactacg gatcaaaacg gcagctggtg
     5401 gtttcatcct gatgaccgcg tttttcaagg ctaccgtctt acccatcaac caagtccttg
     5461 gatgggtgat ttcagtcata tgctgtttac gccgatcaat ggaaaactac aggagaacac
     5521 ccttttccat gctcaaagtt cttaccgccc agaagaaagt atattttgcc cgactcatct
     5581 gtcgatctgt caattacgtt atggcatctg ttctacattg atacctagca tgtatggcgg
     5641 aatcttatct attgattaca gcaaaaatga cagcggttta cttttatcct ttccgggacg
     5701 ccatcagctc tttgttatag atccttacac agtggaagga aaaatcacac attttgctgg
     5761 ttcagaagat cctgattttt ctttttattt tatattgaga tttgaatatc ctttaacaat
     5821 tgatgagtta gctgtttcca acaaaaatag cgactcgatc ccgttgtatt ttgaacagac
     5881 aaaaaaacaa actatccgat ttgggacatc ttttatcagc tcggaacaag cagcttataa
     5941 cctggaacta gaacaaaacc aaagcaaaga gacttacctt gctcacagtc aagaacaatg
     6001 gcagtcctat tttgatcgta tacagctcac acaccatgat caacaacaat tgcggacgtt
     6061 ttatcataat ttatatcggg tgtttttatt tccacaaaca ttttatgaaa ttgatcctag
     6121 tgggcaaaaa atccactatg atacgactag tcgatctata aaacctggtg ttctctatac
     6181 aaataatggg ttttgggata cgtataaaac ggtttatcct ctttattctt tgattgctgt
     6241 agaaaaatac gaagaaatgc tggaaggttt tttgaacagc tatagagaaa gtggctattt
     6301 gccaaaatgg ctgtctcctg atgaacgtgg acttatgcct gggacattga tcgatgcggt
     6361 cattgcagat gctgctgtaa aagatatccg acgagacttg atgcctgaat ttctaacggc
     6421 aatggaaaaa ggtgctacgg ttcaaagcaa acatccaaat tatggacgcc aaggaacaga
     6481 agattacttg acatatggct atgttcctaa tcactaccac gaatcagtca atcacacatt
     6541 agactatgct tatagcgatt tttgtattag ccaagttgcc aaggtactag aagaagagga
     6601 aatgacgaag aaatatcagc agcaatcatt gaactatcaa aatatctttg actatgagtc
     6661 aggatttatg cgttcaaaag ataaagatgg caagttcaga gaaccatttt cagctaatcg
     6721 ttggggacaa gattatgcag aaggaagtgc ttggcaaagc agctttgctg tttatcacga
     6781 tttttcagga ctgatcggaa aatatggtgg cagtgagaga tataaagaaa aactcattga
     6841 gctgtgcaat cagcccccaa cgtttgaccc aggaggatat ggttttgaga tccacgaaat
     6901 gagtgagatg gccgcgatag aatttggaca attagctatt tcaaaccagc caagttttca
     6961 ctatccttac ttgttcagtt atataggaca acctgaaatg gcacagccac tgcttaaaca
     7021 gttgatgact cagaccttcg acgattctcc aatgggttac cctggagatg aggacaatgg
     7081 aagtatggcc gcttggtata tcttcaatag tctgggattt tatcctgtca cgcctggaac
     7141 cggtgaatat gttatcggta tgccattaat tgacaaggct gttttgcttt tgtcaaatgg
     7201 aaaggaattg acgattgaag caacgcctaa tcggccgcag caacaattta tccataaaat
     7261 caaacggatg gacaaagaat atacgaaatt atattttacc catcaagatt tgctgaaagg
     7321 cggcacgatc tcttaccaat tagggatcat ccctcaccct aagaaatatc aagcaaaaga
     7381 tttgcctttt tccttatcaa actataatta acaagaggtt gtgaaaaagc tgtcctgcat
     7441 caagtaataa gaacagccaa gcaaaaatag atcctcatat ttttcgtttg gttggtctta
     7501 ttaccgcaga tgcaagcttt tgaacaccgt ttattgagag gttgtgaaat cagcgttctg
     7561 acctgagtat tagagagaca aatggcaaaa tagttcctca tattttgcca ttttttgagc
     7621 taataccgag ggtcagctga ttgaacaccg tttagtggag aggtcgtgaa aaagctgttt
     7681 agtttcgagt attaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg
     7741 gcttaatacc gagaaactgg cttttgaact ccgtttatcg agaggtcatg aaaaagctgc
     7801 cttgcctcaa gtaataagaa cagcccaaaa ttagatttct agacaatttt ttataagtta
     7861 tttcattaca ttggcttttt cttttatgat tctccactgt tttctcggta ctctttaggg
     7921 gatctgccga cgattttctt gaataattta gagaaatacg tggtgttgtt gtagccaatc
     7981 tcacttgcta tctcattgat gttttgatta ctgtgcaaaa gcagttgttt cgctttttcc
     8041 atacgtaaat ggttcaaata agctgaaaaa ctttttttcg tttctttttt gaacagctgt
     8101 cctagataca tgacatttaa atgcaggttt tctgacactt ctttgagtgt aagtggttct
     8161 tgataatgct ggtgcaggat tacgattact ttttccacat tgtcactgta ctttttctgt
     8221 ctctgatgct ttttgatgaa tgacagaagt agctgctgaa gttcttgcac cgtttttgcc
     8281 tgatggattt tttcgattcc ttgtaaatat tcctcatctt ccagatcaat caattcccgt
     8341 tgaatctcca taaacagtaa aaaactggaa tatcgtatat cttccggcat catggctgct
     8401 aactgaaact gttcaaaaaa atcagtgatc attttttgag cgacttctaa ttgtttattt
     8461 tgcagcacgc gtctgaaact tgaaaaatcg atggtttgat cttttgtttc tgcttgtact
     8521 gcgtaaaaaa catgctcgtc tttatctcca taaaattgat gcaactgtag attgtctttc
     8581 gcttgttgga atgtttcagg gattttgtcc aaagaatctg tctcttcccc gatactgatc
     8641 agccattcct gatcaggaaa gttttcttga acaaaacaac agaagccatc aacttcatct
     8701 gcctccaaca acaacgtgat caatacacgg tctccgtaat ttcgttgata gtaaaagatt
     8761 tgttgccgtt cttgcaacca tctattgata agagtatcat gagttcttgc cagttgaatc
     8821 aatactatcc tatgctgctt ctcacttatt tgtcctatta tttcttcttc acttatttca
     8881 tccaattctt cattcaacca ttgactgaat aacagttctt gatagatttc ttgttgattt
     8941 ttttgttcat tttgctgatc taaccgtgtt ttgattttct tgaggctttc aaccaattcg
     9001 aacttattca cgggtttcat caaataattc acggcgccta attgcatacc tcctttcaaa
     9061 taatcgaatt cttggtatcc tgataaaatc ataaattcaa aattatgacg ttctttttga
     9121 gccgcttcaa taaattccag tccatttatt tctggcattg tcacatccgt tactactaaa
     9181 tcaatctgtc gctgttcaag tacagctagt ccttctttgg cattctctgc tgttgctact
     9241 atttgaaagc ctaaactttg ccaatcaatt atctttttta acccattcaa aatcatgtat
     9301 tcatcatcta ccaataacac ttgatacacg ctcatccctc cttgaatgta atagaaacgg
     9361 tcagtccacc tgtctcatta ttctcaagcg acatataata actgctgccg aagctggcac
     9421 gtaaacgttc attcacgttt tgcagaccga tagaaccgtg aagttccact ttaggatgag
     9481 acagtttttc ttcaatctgt tttagccgtt tttcagtcat cccttttcca ttatctttga
     9541 tgatgatcct tacccgtttg ccttcctgca agactttgac acttagcgca ttatcgaatc
     9601 gagtaaaatc aataccgtgt ttaaaataat tttcaactaa tggctggatc acaaatttag
     9661 gtacttcgat cttttccaaa tcaggatcga tcataaaatg ataggctact cgattaggat
     9721 aacgcatttg atacaaatag acatattttt cgcaaaagtc cagttcttct tttaaagtga
     9781 tcgttttctc ttgattcgta ttgtttcgaa gcaaagcgga aaacgcatac acaacatctg
     9841 ctaattcttc acttccttcg ctgattgcat acattcggat atattctaat gtattatata
     9901 aaaagtgtgg attgatctgt gcttgaagcg cccgcatatg tgcgtcttgc tgtttgattt
     9961 ctaatttgta gatgtcttct acaaactgat tgatgctatc caacatttcg ttgataccga
    10021 ttgaaagttc cttcagttca tattccgtgt ttgttatgtc gatccgcgtc tctaaattcc
    10081 ctgctgctgt ttgatccatg gcctgcatga cgatagatac ttgctgtgaa tatcgtttga
    10141 atgttttata aagaaagata agcagtatat tgtctaaaat aatgatacca atgatcagcg
    10201 gggcaagatt ttttcccgtt tcgattttta cacttttttt atctagtaca gctaatatac
    10261 gataatcatc agacagttct tggtgttgaa caaaattgtt tttttccaga tcgtcaatcg
    10321 gcaattgtga atttttcttc agactctctg tgatgatcat ttcttgtctt tcaaacgttt
    10381 ctttagctat tttattatca tggaatgtgt atagtcgatt tgtcgttcca gaaatcatgt
    10441 ataaagagag tccattgaat gacgttagat tttctaaaat catatctagt tctttctttt
    10501 gaaaaccaat aaaaatactt cctgactcag ctcctccttg tgtgataggc aacttgatat
    10561 aaaaggcgtc tagtaatttt ggtgtcccct tttttatttc cccacctttg ttttctctag
    10621 aagattcata atactccggt agttgattca acacgatata tatcgcactt agttcttcat
    10681 aatttgcgta gagatctttg atttgatttg ggaaagaaac taattcatcg gtactgtttt
    10741 gctgttcgta tacatacatc agatactgat ctatgggttt tgttaagtag gtcgtcacat
    10801 tttcgatctt ttcggaattc cctacaagct ggtttttgat gatcctactc aaggttgttt
    10861 tatcattcag cattctgctt gtgctttgaa cggcatcact tgtcatgttt tctgcctgtt
    10921 tttgattttg ttcccaagta tggatactaa aaataacggt agcagtcatc gttacaccga
    10981 tcatgataac cgcgtatttc cgcaacagac gatttagtaa ggttcctttt gaaaatagaa
    11041 tccgaaactt attcataact ttccaactgt ttatctaatt cttcacgcca atgtgttgtc
    11101 atcgtaccat tccaaaccgt caaaagacca atgacaccaa acaggatcaa tcctttgaat
    11161 tgccaagtca cgcctaaaat tataccgcta ccaataatta tttttagaaa agacgaaaag
    11221 ctggaaaaaa ccgaaataaa acttagcttt agcagattag gaacagagat cgtatattca
    11281 ctatctaaaa tcatactgta ttgataagta gcaaataggc agagcaaggc aaaaagtaaa
    11341 ataaaatcaa tagctaaaaa aatgagccct ttgatttgaa cagataaata t
//
LOCUS       DASNNU010000082        11348 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.082, whole genome shotgun sequence.
ACCESSION   DASNNU010000082 DASNNU010000000
VERSION     DASNNU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.082"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(56..634)
                     /locus_tag="U8116_002149"
     CDS             complement(56..634)
                     /locus_tag="U8116_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN8435113.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(654..1322)
                     /locus_tag="U8116_002150"
     CDS             complement(654..1322)
                     /locus_tag="U8116_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8435114.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1337..2407)
                     /locus_tag="U8116_002151"
     CDS             complement(1337..2407)
                     /locus_tag="U8116_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8435115.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2578..3942)
                     /locus_tag="U8116_002152"
     CDS             complement(2578..3942)
                     /locus_tag="U8116_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN8435116.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(3939..5498)
                     /locus_tag="U8116_002153"
     CDS             complement(3939..5498)
                     /locus_tag="U8116_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8435117.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5554..8034)
                     /locus_tag="U8116_002154"
     CDS             complement(5554..8034)
                     /locus_tag="U8116_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435118.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(8018..8353)
                     /locus_tag="U8116_002155"
     CDS             complement(8018..8353)
                     /locus_tag="U8116_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435119.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(8367..9401)
                     /locus_tag="U8116_002156"
     CDS             complement(8367..9401)
                     /locus_tag="U8116_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN8435120.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(9491..10171)
                     /locus_tag="U8116_002157"
     CDS             complement(9491..10171)
                     /locus_tag="U8116_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN8435121.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(10341..11300)
                     /locus_tag="U8116_002158"
     CDS             complement(10341..11300)
                     /locus_tag="U8116_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN8435122.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
BASE COUNT         3229 a         2301 c         1992 g         3826 t
ORIGIN      
        1 gatcttcgtc atatcttgct tcttcaccta actattttct ccctatataa cagctttacg
       61 cttctcctaa tacaccattc aaatataaat cgatcaattg cgccacattt agcggttctt
      121 ccacaggtct tttcgaccga gtgatgtaag gtgctaacac taagaaaaaa tgccttgctg
      181 cgatttcttt tgaaatccca tttcttagca cagattggtt tgctgaaaag atttcttcaa
      241 aaggttcacg ataatttttc ttccatagaa agaaaagatg atctcttgtt tcctttgaaa
      301 gattgctttc aagatcgtga agcatcagaa acaaatcgac cagatgtgtc tcaatcaaaa
      361 actgcgccat ttttgtcaag gttacttgaa atcccgtttc gcttgtttga acgatttctc
      421 tcaaagcttt tccaacatct tttaagtggt tttctaaaac tgccgtatat agtttttctt
      481 tatcaccgaa ataatgatag agattgggct gggtgatccc aactttttgt gcgatttctc
      541 ttgttgaagt gccgagatat cccttggaca taaacaagtc agtcgcttct ttcaaaattt
      601 tttgctgtgt atctttgttg tatcgatgga ccatttttcc acctgctttt tggttattct
      661 ttttctttca atacaccatc ttccatcata aatattctat cgcatttgtc aatcagccgt
      721 gtatcgtgag taaccatgat cgttgcttta tttttgtctt ttgattcttt tgccaagata
      781 tcgactactt ctcgtgcctt ttctgaatcc aaactagcag ttggttcatc tgctagaata
      841 atggacggat cattgtacaa tgcacgagcg attgcgaccc tttgccgttc tccaccagat
      901 agttcttctg gatacttgtt gatcaactta tccacgccaa gctgctcaaa taattcgttt
      961 gcttcttttt gacgatgttc atgtttaatc ttatctacta ataataactg cttttttact
     1021 gtcagaaaag gaaccagatt ggaagcttgc aaaataaagc caatttcttt aaaacgtagt
     1081 ttaacgcgtt cgctttcttt ttgattactg aattcttgat cattgataaa tacagagcca
     1141 ctgctaggcg tctgcaatcc gcctgccaaa gtcaaaaaag tacttttacc tgaaccagaa
     1201 ggaccgatga ttgcaacaaa ttccccttta tcgatactca tggttgtttc tttcaatgct
     1261 ttaactactt ggtcgccatc ttggaaactt ttttctactt ctttaaattc tattgctttc
     1321 atgttcttcc tccttcttat ccaattgcct tcaacggatc gatttttacg attgtccgaa
     1381 cagaaaatag tgcgcctaac acagctacta ctaacatcaa caagctaatg acgccaaaaa
     1441 acagccaatt gctttggaat ggaaccgcat caggtagaac aagggatgtt cccactgttc
     1501 ctaatagacc aagaagaata ccaacaaatg ataaaatgaa tgtttgtgcc actacagaac
     1561 gcgcaataaa tcctcctgtg atcccttgtg ctttcattac accaaagata ttaatctttt
     1621 gcatcgtcag tacataaatg aaaatcccga tcacaatagc cgcaataacg atcaaaaatc
     1681 cgatcataaa tccaaaagtc aatacttgcg cattatatcc aggtaattca ttgatgaatt
     1741 tagaaatttt gatctgttct aaatcttcag gtaaattact gattttacct ctgacaacta
     1801 ttgcgttaat ccgtgcattt tcacttgtat cttctttttc gaagcggatt tcttggtaag
     1861 cattgatggt tgtatagaga actggtgaga cattgaattt ggcatgatcc gtaaagccag
     1921 ttagtttaaa cgtcttgtca ctaccagata acttaactgt atctcctatg gctagaccat
     1981 attcttcttt caaactaata tcgcctactg cctcatcatc gttatcaaaa gcttttcctt
     2041 caacaagatt gggcatgatg aattgatttt tatcgatacc gaagaagcta acattgattt
     2101 ttccttcttc tgtactgtct ttactagtca cgactccagg tgtttgggca agatacgcca
     2161 ctttcccacc ttcgacttct tctgctatct tcttagtaat catggacatc cctagatttg
     2221 aatttgcatc tttcgaaagc acaatcgaat ctgcctccca cttgtcgact gctgttcgat
     2281 tatcttgagc taagccataa gctaacccag tcaaaaaaaa taccaggtag gcgatcaaaa
     2341 acatcacccc tgcaactaac gcataacgta actttgaatg cataatttca tttaatgcta
     2401 aaaacattta actcctcctc ctgaaataat cattttatac aacaatttta tcacttgata
     2461 aatttttggc gaatattatg ctcaaaaaat tcttccgcta tttcattata tgaatttttt
     2521 attctcgatt gattaatatt tattatatcc ccacacaata aaagaccgtg atatttctca
     2581 cagtctttta tctactctat tgctatttgc tctcttagct agtactttaa aagctaactc
     2641 attgaggtag tgaaaacgag aggtgtattc agacagaaat ccaaaatcct caactgctag
     2701 ctgtggaatc gtagttttgt gtttcacgat atccgcaagc aggttcaaat ctgataataa
     2761 gaattcttgg cttatcagct gtccgccaat tatttctaat gtatccggat ggtagatcag
     2821 ctttgcattt actgttttgt tttcttcgtc gtattgtgga aatagatcaa attctcctga
     2881 acacgaaccg gtattttgtt ccaagaatgg cgcttcatct tctgtcagcc ctacagttcc
     2941 taaaaagtag tctataatga ctgctgctgt tatcttttgt ctgaggtcgt aagcggttgg
     3001 ctgaccactc aaagttagcg ctactgctct tgccatatgt acagctcgag taactaaagg
     3061 taaaaagaga tgccgtttat ttttgtcaaa actgactggg acgatatctc caatcgcata
     3121 gatgtccgga tcacttgtct gcagatattc attgactatc acagtctcat cctcatacaa
     3181 agccactttt tcttttaaca atgtcgtatt aggacgagtt tgggcagaaa aaatgaccat
     3241 atctgccttt attgtttgag acagaagttg tacggatact actttctctt ctgatttttt
     3301 ccaatcgatc aagaattcat ttggataaaa atttatttgc ttagtttgca gctgattttg
     3361 aaaactgttg atcatatcct tatcgaaata tcttgataaa acattcggca tataatcaac
     3421 tagatgaata atgtatcctt tatttctcaa agcatctgct aattccaatc caatgtaacc
     3481 agcaccgaag atcactactt cttttgcttc ttttaaagcg gcaagtactt gcttagattg
     3541 cactagattt ttataggtgt agacattttc agcttcaatt tgtttataca aggtgacagg
     3601 agaggaacct gtcgctacga ttaatttatc ataggttatg gtctcgtttt ttccttcttt
     3661 ggatgcgagg atgagctgat gtttgtctgg atcaagttca gttactctcc attcggtgat
     3721 cagttttgcc ccactttctt gaagagtccg catgttatgg gcaacttcag ctaaactacg
     3781 tatctttcct tttaaataaa ggttgattcc atttgaaaca tagcctagtc catctttatg
     3841 atcttcatcg atcagtaaaa cttctgcttt cggttgaagt tttcttagat ttagggcagc
     3901 tgtgatgcct tcatgggaag caccaatgat cacgattttc atgttttatt catccttaaa
     3961 ttcaattttt ctaaataaca ttcttttaaa aattggttca aataggggaa atcataatta
     4021 tattcgttgc tagtgaatac aaaaacttta gcggacttct gtgacgggta ctctttcaat
     4081 tggctagtca aaatgatatc atactgcttt tctttctcgt attcgactaa atcatatttg
     4141 atcgtattca gcaattgttc ctggatgatt tctttcacga tatccgccat tagcctttca
     4201 taaggaagat cataagcaat ttgcagacgt aattctaact gcttgatcag cgtccgtaaa
     4261 atagatgcat agcgagcaaa taattcaaag cgggcatttt cctcgtctac ttcagtaaaa
     4321 tcaacaatag caaatgtttg tctgaccaat ctttcacata aaaccaggaa ttgtttatct
     4381 tctacttcag ttaaccgctg ttttaatcct tcttggccaa aaatattcat ccatcctcta
     4441 aaataggcaa aatgagcgtg attttgcatc actgtcaacg caatcttttg cgaaatctct
     4501 tctggaaaaa cataaaaaga gaaatattct tctatcgatt gtttaaacgt tcgattcaat
     4561 tgagctacta cgtcgtcttt gtatttttcg cttatcaaaa actcctggac atgcgagtta
     4621 tgtgtatcta atgtaaagtt actgatcatg aaaagataaa aaaccaacgt ttcattttga
     4681 ttccaaggaa aggcatattg aaagaagtaa cgggtcaaaa atgtatggag gtccaataaa
     4741 aattgtgtcg tttgaaacaa tccaacagga agattgctat aatctggttt ggattttttc
     4801 gtacagcgca ttcgcttctt cataatggaa agccatattt caaatttcaa tcgtccttct
     4861 tctgtaatag caaaactaaa ctctttttgc agcagatcta aaaaagtgtt tcctgataaa
     4921 tcactgtatt cttttttgtt ttcaagatag ggacgtccaa accaaaataa ttggaaataa
     4981 aaataactaa tttgactttc tggaccggtc agccgcccgc tttttatttt cagatgaaac
     5041 tcatttaaag aatcatttaa ttccttcact ctgcgagaca aagtagccga actgatatta
     5101 agttccatga caagtttctg ccgatcaaat gagccatttt ccaacagata aagaagtatc
     5161 tgatattttg tagaatagcg caagtaacaa tgataataaa catcgactgc tgctatattt
     5221 ccatgtctga ttaataaaat attcccttct tggataacaa tttccaattg tttgatattt
     5281 tccaagttag cttgtaaata aaacagatct tctttcaagg tagcccgatc aactgataag
     5341 cgggtaagta cgttattaac ggaaacctct cctttttcct ctaataaaag ttggaaaagt
     5401 ttgatttgcc gttgttcatt tttttcaagc aaactgaaaa gttccaagtc cactccttca
     5461 agcatttctg attttaacag cagaagtttc cttctcatcc ttgaaaaaga ggatctgtga
     5521 cagttatcac agatccatac aaattctaat cacttagctg gttggcccag ctgtcagttc
     5581 ccatgtgacc atgccttgat acgttgcgcc aggttgagca ttcgtactgc tatttacctc
     5641 taagagtaaa ccatcggtat cgggaaagtc aacgagaacc tctcctttag ctgttccatc
     5701 actctcataa atcaacgtgt tttgaccttg ctgaatcaat tgctttgatc cagaatgatt
     5761 gaagtataat tttgttccgc ttaattcttg accttgatca ttcttaaata aagaagtcaa
     5821 tgtacctgtc agttgccaag gagacgtgct tggaagccga gtatcccgca catcaaaaga
     5881 aaaatcgcct tttgattcat aataacttgt cttcaacggt ttggggagat cgttaccaaa
     5941 tgtaaaatca tctggtacat taactaatgt taattcttgt ttggggacaa gaattgtttt
     6001 agcagatgtt tggacaactg ggttaggaag cttagcacca tctgctaatg taatattgct
     6061 ggaaagtaca gctgcctttg tcagtatgct gacggattgt aaagaagtat ctacagtatt
     6121 taattgatag ttgtaagtaa gatgaatggt atctcctatt ttgctcaata gatctgctgg
     6181 gacagaaact ccttgttgaa tgccggtatc tgcgatagtt gtaccattta attttaagga
     6241 accattgata taagtagtat tttcaggaag tgagatcgtg atttttttgt cggttatcgc
     6301 atccccagat tttgttaacg tgaatgtcac attttcttga atcgtatctc ctgatagagc
     6361 agacagggga tctcctgtct caggttgtct gctaaggttt tttagctgac tatctgcact
     6421 cacagatgga aaattagcca ctgttatttg aggaagagtg gtgattccag cttgttcaga
     6481 ctgccatttg tacgcaaaag acggatacat gttagtaagt acatcgtcca ccaagcgata
     6541 atcaaaataa tattgccctg aagccaatgc aggtagtgta gaagtagctg tatttacacc
     6601 cccgttattt gattggatcg tttggaaaag ttgatctgtc catcctgtcg ctaatttttg
     6661 ggttcctttc gaataaagcc tgtaatggat cgaacccttt tttagttcag ttgctgacca
     6721 attggccgtg acttggtacg atgcagaaga aaggagcttt ccccactgac tatcggctgt
     6781 taagtttgtt ggtaaaaccg ttccgtctag cttgtactgc tccaatacct ttaaagcaac
     6841 aggagaaagc aatccgcctt gcgcatcttt cccactgtac acgatattgg ctgtctgatt
     6901 caatccactt gtgcttgctg gagtatgagt cagtattgct ccttgtgtaa cgttttttcc
     6961 tgtaaaatag ttgactacgc gatcaaagcg actaaatggc aactgatcaa aacgtccttc
     7021 agtcagcccc atcgtcaatg tccgactatt ggtagagcct ggaaggttct ggttatttcc
     7081 atccacgtat tctgtggtat cagcaaatgt attatatttt cctgttgaat cacttggtga
     7141 gatgtaacta ttccacgtat ttatctctgc acttgcagaa aatgcatatg ctcctggatt
     7201 caagtttccg ccagttaatt taggataaaa tctacctgca gctggagcta aaatcgaatt
     7261 gaacatacga tcttggataa tattattcga atcataaaaa tcaacagaaa aaacggcctt
     7321 tttagacata gaaggttgta atttaccctt ggtcaaaatt ttgtttggac tcgtatcata
     7381 tttatctccg ttttctgtca tcttcgcgac attagctgta acatcaaatt gaagcgggat
     7441 atcgtccacg ttgaattcgc ctcgcgtaat aatagcaata atatctgtca atagagtatt
     7501 tgtatcccac gaccccatcc caaaacgcaa ggtatggctt cctgctgata ataaacttcc
     7561 gctagtaacc ttgatggggg caccggcaat atacaagtag gcgttgttcc agtcaattgc
     7621 ttttaataca tcactgcttg atacatgttc cggcaaattg atgatcgcca caaatgcaaa
     7681 tccttgtgca gtgatattca ccaaatcaag tacatttgtt agtttcacat ggctttccca
     7741 atgaaaacgg accgtgccac ctttccaagg atcatttgaa ccatcgttca aataagtagc
     7801 agtcaattta ggcaattggt tagccccagt tagttggaca agcttcccgc cattgactgc
     7861 actacgtcca ccatttggag gtacgatatt ttcatacgtg tatggatcat aagaaatggc
     7921 aggaggcgtt gctccaacag cttctgccga aggtgaaaag ataaatatac ttaaaacaaa
     7981 aagcgaagta aaaaagaagg agcgaaaaaa tgaaaattta ttttgtagca acatgttcat
     8041 cctccttttc ctttcttctc cataacaaca accacgcaag aagccataat tctataccaa
     8101 tgatcaaata aagaaaacgg atcttttgtt cattcgtctc cgggaatacg ccatgttgat
     8161 tagattcttt ataaatcggt agctgtgtct gctgttttga tgcgccattg actgaaccac
     8221 ttggatgcgg aattggactt agatcataaa atgtaatcgt acctgaactt ttggtatctg
     8281 cttggactgg ctcttgccag ctgaagaaca gcaaacttgc aataatccct cccaataaga
     8341 tcgatgtttt caacgaaatc acctcatcat gttgtttctc cttttttctt tttcttgtgg
     8401 acccacaaaa acagcaaaat caaccataac actagaactg cagcaaccaa tcctgcataa
     8461 agccatggga aagattgttc ttgtactgca aaaacggaat tttcatttac tttttgtgct
     8521 ttttcttttt tgatcgtaaa gttttgtgta aatcgccatt cattactgtc gcctgatttt
     8581 gctatgattt ctgcgcggta atcgccggct tttgctttat caccagctaa atcaagattc
     8641 aagtgaaaat tcgaatttgg tgcaactaaa aatgcattag acgtattttc aatataaagc
     8701 tgattttcat aaaagatctt gctcgtaaat atcaaattgc tgataatcct aggctgagga
     8761 ttttggaaac gaagctgaac agttggcttt gcgttgacct cgtccaaatc tcctgataaa
     8821 agtttcagat ccggttctat cgtctcttga ctttcttgta aaacgactgc gagattataa
     8881 gcaatacgat ttttgatttg tgctccttct gtagcatttt ctgtttcagc ttcttcttta
     8941 ggtgaaacat gaatacccgc taatacacgt cccttaaagc tttttgctgg gattttcact
     9001 tctataggta cagttgtttc tgcatgtgct gatacattga tttccttttt cgcaatgagc
     9061 aaaacgtcac ggacatcaaa cggcaatgtc tcatctaacg ttgggttttt ttgactataa
     9121 tcgatcgttc caccatcact cgttaccgcc ggattgacag agacctcaaa tgttcgttct
     9181 tcatctgaag aattgctgat aattacttct agtgtctgat tttgttccgg tgccactaat
     9241 aaatcaaaat aaccgattgc tttatctatt tggttctctg gaaataccgg tttgattcca
     9301 aagtctcctg ctgaatcctt tgcttggctg attgacggaa aaatgaaaaa tagacttaaa
     9361 atagaaaaaa acgaaacaaa cagtcgttta ctttgattca ttgtccacca catcctttca
     9421 acaacaactg cggtaaaaaa tccttcacct atagtatttt caccgcagtt tgttgaattt
     9481 atttcatact ctatgctact gggccagcca cgaggttcca agtcaaatca gccgtgaatg
     9541 ttccagctgc tgctttcggc actttcagtg tgatatttgc tggatcataa acagaaagcc
     9601 atgttcccag accttgatct tttgctgctg taaatattgg tgtcgctgca cttgctttac
     9661 tcaacccagt tacagtttga ctagttggtc cagtggattc gttatctgac gtacttttaa
     9721 cagtggatgt tccaaaatct aattcagcgc cttgcaaaac ttgtgaaccg tttacaaaat
     9781 ctgaaaccga aactgtcacg ttccaccctt gtgcttggcc atctgcacct acacctcgac
     9841 gatctgatac ttggagataa ggtgtatcgt tttttgaagc gtctacttga tacgtttttg
     9901 ttccgctttc aatctcgtgt gtaccaaatg gcaattcggg aactacatcc agtgttaaag
     9961 gacctgtctg gccagttgct ggattttccg gatctgctgg ctctactggt tttgaaggat
    10021 cagtagggtt aagaggctcc acggtatttg ttggttcagt aaaggtaata tctccttttg
    10081 aagtcgcttg accctcagca aaagctggtg ttgccaagcc gcctagaaga agtgttgaaa
    10141 gtaataatgt cccaatcatg ttctttttca taataaatca ctcctttttc ttagtgacct
    10201 aagtatagta gagacagtat gcaaaaaatc ctcatgaatt catccgctta cttgaaatta
    10261 tgaccctttt tgtataaaag agaaaaaaac aataaaatcg tttacatgaa tattcttcct
    10321 tttttattaa gtttcgtttg tcaaattaag ttagacaaat catcttttgt gacgtaactc
    10381 aaaaatactt ccattggtgt ccgatagtgt aatgattttc taggaatatg atttcgtttc
    10441 gatgccaccg attggataaa ggtttcagat acaccattaa aatccatagc tttatacaaa
    10501 ccatctttac gtaataagcc atttgaatgt tcattcaatc ctcgttgaga aggtgttcct
    10561 ggatccgcaa aataaattga aatatcgttg gtattactaa tctttttcca atttgagaat
    10621 tcttttccac aatcaaatgt gatggattta aataagtttt ttgggacaca acgaaaccat
    10681 tgattcagcc tattttcaat gtctttagcc tgtctgcctt cgggtttcaa ggtaatgatg
    10741 actttagata gccgttcaac taatgtgatg accgcgcttt tatgttttcc acccacgata
    10801 gtgtcacctt ccaggtgacc aaattcatga tcaaactggg tatagtctgt ctttctgtct
    10861 cggattgacc ggcgaaaccc ttgttttcct cttttttctt tatgaccgtt tggttttctt
    10921 tttcccttca ttggtaaatc gtgagtgtca aaggttttat ctttaaacat tcgatacagt
    10981 gtgcgtaccg aacaagcaat aggaaactct gctcgaccaa taaggacatc aggagcccac
    11041 ccttgaacaa cctttttttg aatgtattcc tgttgctcat caggtaaaac aatcggatgt
    11101 cttccgcagt tacgtttgtt tttcttatat tgtttatagt agctcattgc gcttccacca
    11161 gatttaagaa aacgatagac cttataaata gtttgtcgag aacgttgtaa cagattggct
    11221 acttttgaaa caggttgtga ttgattgaaa taagcttcta tcattacgag ttcagttggt
    11281 gtaagatggt tataggtcat ttgtactcac tccttataat tctttcgtcg gaactatttt
    11341 gagtgtag
//
LOCUS       DASNNU010000083        11197 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.083, whole genome shotgun sequence.
ACCESSION   DASNNU010000083 DASNNU010000000
VERSION     DASNNU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.083"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(13..906)
                     /locus_tag="U8116_002159"
     CDS             complement(13..906)
                     /locus_tag="U8116_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8435123.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="U8116_002160"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="U8116_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEN8435124.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="U8116_002161"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="U8116_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEN8435125.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="U8116_002162"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="U8116_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEN8435126.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="U8116_002163"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="U8116_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEN8435127.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="U8116_002164"
     CDS             complement(6343..7062)
                     /locus_tag="U8116_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEN8435128.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="U8116_002165"
     CDS             complement(7059..8372)
                     /locus_tag="U8116_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN8435129.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="U8116_002166"
     CDS             complement(8453..8773)
                     /locus_tag="U8116_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN8435130.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="U8116_002167"
     CDS             complement(8881..9168)
                     /locus_tag="U8116_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435131.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="U8116_002168"
     CDS             9295..9939
                     /locus_tag="U8116_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEN8435132.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="U8116_002169"
     CDS             complement(9998..10705)
                     /locus_tag="U8116_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN8435133.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="U8116_002170"
     CDS             complement(10925..11095)
                     /locus_tag="U8116_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435134.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1956 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaac
//
LOCUS       DASNNU010000084        11189 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.084, whole genome shotgun sequence.
ACCESSION   DASNNU010000084 DASNNU010000000
VERSION     DASNNU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.084"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            151..1410
                     /gene="lysA"
                     /locus_tag="U8116_002171"
     CDS             151..1410
                     /gene="lysA"
                     /locus_tag="U8116_002171"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEN8435135.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1397..2197
                     /gene="dapF"
                     /locus_tag="U8116_002172"
     CDS             1397..2197
                     /gene="dapF"
                     /locus_tag="U8116_002172"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEN8435136.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2200..3081
                     /locus_tag="U8116_002173"
     CDS             2200..3081
                     /locus_tag="U8116_002173"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEN8435137.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3078..3794
                     /locus_tag="U8116_002174"
     CDS             3078..3794
                     /locus_tag="U8116_002174"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEN8435138.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3800..4495
                     /gene="dapD"
                     /locus_tag="U8116_002175"
     CDS             3800..4495
                     /gene="dapD"
                     /locus_tag="U8116_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEN8435139.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4511..5512
                     /locus_tag="U8116_002176"
     CDS             4511..5512
                     /locus_tag="U8116_002176"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEN8435140.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5509..6870
                     /locus_tag="U8116_002177"
     CDS             5509..6870
                     /locus_tag="U8116_002177"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEN8435141.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6830..7981
                     /locus_tag="U8116_002178"
     CDS             6830..7981
                     /locus_tag="U8116_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEN8435142.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7956..9125
                     /locus_tag="U8116_002179"
     CDS             7956..9125
                     /locus_tag="U8116_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN8435143.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9300..10679
                     /locus_tag="U8116_002180"
     CDS             9300..10679
                     /locus_tag="U8116_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN8435144.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10960..11189
                     /locus_tag="U8116_002181"
                     /pseudo
     CDS             10960..11189
                     /locus_tag="U8116_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3729 a         1952 c         2497 g         3011 t
ORIGIN      
        1 aaccattttt attcttattt ttaaaaaaat atttcgaaat atactagaca attcagaaaa
       61 ttttgtttaa tattattcca ataaaattca aatctttatc aaatgaaatt ccaataaaaa
      121 cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat tgataattgg cggagtcaca
      181 gcaagcacat tggcagaaaa gtataagaca cctctgtatg tgtatgatga agaaaagatg
      241 agacaaacga tgctgtcatt caagcgaggc tttgtatcga aacgttttga tacaaaagtg
      301 ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc atttagttcg tgaatatgga
      361 ttcgggctag atgttgtcag tggtggggaa atctataccg ctttgcaagc tgattttcct
      421 agagatacta tctacttcca tggtaacaac aagacgccag aggaactgat ctatgcactt
      481 gaaaatgatt tgttgcatat tgttgccgat aatttgatgg aagttgaatt attagcagaa
      541 ttaagccaac gctatttaaa aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa
      601 gcacatactc atgaatatat cgtcactgcg catattgatt caaaattcgg catgagctat
      661 gaaagtaaag agtgccaaca agcccttcag ttgataaaag aaagcagcta tctagagtta
      721 gaaggatttc atgctcatat cgggtcacaa atttttgatg ttactgcttg gttagctgaa
      781 atcgacaaat tgacgaatta cttaaccgat tttgaccaac ctctttcctt gaatcttggc
      841 ggagggtttg ggattcgtta tacacaaaat gatcagccga tcccagtcga agaggcacta
      901 aaaaaactga ttgaatatac ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa
      961 ttactgatcg aaccgggtag aagtattgtt ggagaagctg gtacaacact atacacgatt
     1021 ggtttcatca aaaagacacc acataaaaaa tattactttg tagatggcgg gatgacagat
     1081 aatatccgtc cagctctgta tcaggcagaa tacgattgtg atttagcagc aaaactgtcg
     1141 gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta
     1201 attaaagaag cttatttgcc gaaagcagaa tcgggtgatc ttttagctgt ttattccacg
     1261 ggagcttatg gatactcgat gagcagcaac tacaatcgag cgacacgacc agcagttgtt
     1321 tttgtcaata atggccaatc aagactgatt gttcgcagac aagcattttc tgatctacta
     1381 aggggggaag tttcctatga tccttcataa atataatggc tgcggaaatg attttatctt
     1441 attagattac gcagaagaca cagattatag tcaattagcc gcttctttat gcacaaaaga
     1501 aaaatatgat atagacggat tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta
     1561 caacaaagat ggcagccgcg cgccgatgtg cggaaatggc attcgctgtt ttgctcgata
     1621 cgtaaatgat cagaacatga tgaatgaaca ggaattcaac gtagagacat tagcaggcac
     1681 aagaaaaatt acggtgttgg atccctcacc tttttattgc agcgtagata tgggagtgcc
     1741 agatgattct ccggaaaaaa tcaaagctaa tcaaacttat ccaataaaaa accaggaaat
     1801 agagatcaat ggtgaaaaag taacgatcac tagtttattt ttaggaacga ttcataccgt
     1861 tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca
     1921 tcatcctttg ttcaaagaaa aaaccaatgt caactttgta gaagtagtca atgaacaaga
     1981 attgatcgtc cgtacctatg aaagaggagt aggttggaca cttgcttgcg gtacaggttg
     2041 ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt
     2101 tcacttggaa caaggagact tgatgatctc tggtgaggat gcgatacaaa tggctggccc
     2161 agcagttcat gaatggacaa gaatattgga ggaatgaaga tgataaaagg ttcgatcgtg
     2221 gcgctgatca cgccgatgaa tgaagaggga tcagtagatt atgctggttt ggaaaaattg
     2281 atccaattcc atttagatga acaaacagat ggattattag ttttaggtac aacaggagaa
     2341 tcttctacgc tcacacaatc tgaggaggag cagatccttc aactgacagt aaagaaagtg
     2401 gctggccgtg tgccggtgat tgcaggagct ggaacgaata atacgaaaga aacgattgaa
     2461 aaagcgaagc attttgcttc tttgggggca gacgcacttt tggtcatcac cccttactac
     2521 aacaaaacaa gtgatgcagg actagctgct cattttacag caattgcaga agcctcaccg
     2581 attccgctta ttttgtataa tgttccttct agaacaggca tgtcgattcc aatccatgta
     2641 ttagtaaatt tagcagaaca tcctaatatc attggtttga aagaagcctc aggagatatg
     2701 gcttatgtga tggatgccgc tcggttgatt ggagaagaat tcttccttta cagtggaaat
     2761 gatgatttga tcctaccagt aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg
     2821 aatatccaac ctaaaatagt ccatgaatta gtaaaagata cgcaggacgg aagatggcag
     2881 caagcaaaag aaaagcaatt aaatgcactt gaactgatcc atgccttgtt cagtgagacc
     2941 aatcctattc ctgtcaaagc agccatgtca ttattagact taccttccgg acctctacgc
     3001 ctgcctttag tttctctttc aaaagagaaa aagaaacaat tagctcagct tttgttaaaa
     3061 gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag tggacagatg ggaaatagag
     3121 tcgaagaagt tgcacaagaa agaggagata cggtccttct tttacggacg gcacctaaag
     3181 aagcaactac gcttgctttt acggaactgc cagaactgct tattgatttt tctcatccag
     3241 ataatttaga gaaaatcctt tcgtatagct tatcatatca acttccgtta gtcattggca
     3301 cgacaggtta tacacaatca cagttccaag aaataaaaga acatgccaaa catgtacctg
     3361 tgatgtatag tgctaatttt tcgttcggca tcatggtgat gaatcaacta atcaagcaag
     3421 cagctgcaat gttgcaaggc tggcagatcg aactgattga aaaacatcat agtcgtaaaa
     3481 aagatgcacc atctggtact gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc
     3541 tgcagcccgt atacaactgg caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca
     3601 gtattcgtgc aggttcttta cctggtgaac atgatgtatt gtttgctgct actgatgaaa
     3661 tatttacgat caaacatgaa tctttttcta atcgaatctt tgctgcaggc gcagtagaag
     3721 cagcggactg gttgaaagat cagtcaccag gttattacaa attagaagat ttattaatgg
     3781 acaaaggggt gtaaaaaacg tggaagctca agaaatcatc cgctacattc aaacggcatc
     3841 taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag
     3901 taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt gactatgaga cgattgctcc
     3961 ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata
     4021 cagcgcagtt cctttgctag atattaaaaa aattaatgct cgtatcgaac caggtgctat
     4081 tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc atgatgggag cgattatcaa
     4141 tattggtgca gtaatcggtg aaaacacgat gattgatatg ggtgctgtct taggtggacg
     4201 agcgacagtg ggcaaaaact gccatattgg tgcaggagct gtcttagcag gtgtgatcga
     4261 accagcatcg gccaaaccgg ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt
     4321 gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt
     4381 agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct gcacgtgtat taaaaataag
     4441 tgatgatcag acaaaagaaa atacagcatt gattgctgcg ttacgcgaat tatagaggag
     4501 atggagaaaa atgcgcgaaa tagatacctg tattgtgggg gcaaccggat tagtcggcca
     4561 aacgatgctc aaagtgttag aagaatatga ttttccagta ggacgattga aattattagc
     4621 ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa aagaaacaca ctgtacagga
     4681 attgacagaa acttcatttg aaggatatga tttagcgcta ttttctgcag gtgcttctgt
     4741 atccaaagag tttgcgccaa tcgcacgaag tcagggacta tttgtcatag ataattcctc
     4801 agcatggcgg gaagattcgg atattccatt aatcgttcca gaggtcaatc tggaagacta
     4861 tacattgaat cggctgattg caaatccaaa ttgttcaacc attcaagcgg tacttccgct
     4921 taaagcttta caagaagcct ttggtatcca gcgagtcaat tattcgacct atcaagcagt
     4981 ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt acgcaaaatg acgaggaacc
     5041 agttttgtat ccttataata ttagccaaac ggtgattccg gaaatcgatc agcctttgga
     5101 aaatgggtat acaaaagaag aacagaaaat gataaatgag acccgtaaga ttctgcatca
     5161 gcctgctttg ccagtatcag caacctgcgt acgtgtgcca atcgaaaatg gacatggagt
     5221 ctctgtggca gttcaactgc aaaaagagtt tacgacggaa gaaattagag agtgtctcag
     5281 ccagtttcca gggattcaat tagaagatga tctagcagca cataaatatc cgacgagcat
     5341 tcttgctaga ggaacagata tggtttatgt tgggaggatc agaaaagaca cttctttaga
     5401 gaacggattg ttgttctata cgactgccga taacatacgc aaaggagccg ctgcgaatgc
     5461 cgtacagatt gctgcagctt taatgcagaa aatagcggag gtggcaagat gagtaatacg
     5521 ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg aagagcaatt caaaaaagtc
     5581 cgtacaattg ttctttcaga tcccaatcgc caagtgatcg ttgtttctgc tttaggcaaa
     5641 cgtacgtcag atgatgacaa agtaacagac ttactttatt tgatttatgc ttaccttcag
     5701 catcatgttg attggacacc cctttggaaa aggatttgtg atcgtttcat tcaagtaaga
     5761 gatgttcttg ccctgcagtt acctattgaa gatgaactag cacgcataca gaaacaactg
     5821 gaagacgaaa agataactga agattattta gtaagtagag gtgaatatct gactgctcaa
     5881 ctgatggctg agtatcttgg ttaccagttt attgacgcaa aagacctgat tttctttgac
     5941 tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa tcacaaaagc atttaaaaga
     6001 tatccgaaaa tggttgttcc agggttttac ggggcgaacc ctgcaggaaa agaaaaatta
     6061 ttgggccgtg ggggaagcga cattaccggc gcgatactgg cgaagatgtt gcaagcggaa
     6121 agttatgaga attggacgga tgtttcagga atcatgatgg ctgatccgcg aatcatcgat
     6181 catcctaaaa tcatcgagga aatctctttt agagaactga gagagatggc ttatatgggg
     6241 gctaacgttc ttcatgaagc agctgttttc ccagttcaag aggctgatat ccccatccaa
     6301 ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa tatcgaatca cgaaatagaa
     6361 aaggaaaatg gactgacagg aatagctgga cgcaaggatt ttctttccat cactttattc
     6421 aaacgtcata tgtccgatga aatcgggttt atctggaaag caatgagcat ttttgctaaa
     6481 catgagatat caatcgaaca tattccttca gggatcgaca atatcggagt gatcgtctca
     6541 gcagaagcga ttgccgataa gttatttctc atcacgaaag aattaaaaga aacacttggc
     6601 gtggaagaaa tcgtcgtgat cgaagactta gctttgatct ctgtcgttgg gggtccgcat
     6661 aaagaattga ttggattatc tgggaaagtg ttatcgatat tgaacaaatt agaaattcgc
     6721 acatcgatct tatcacaagg agcacaggag ttgaatctga tcatcggtgt accgaataat
     6781 caatatgaaa cagtagtgaa gggtatctat gaagggatgg tgaatacgga tgctgaaaga
     6841 atatcgaatg gcactccatc aaattcctga aatcgggttt catgaattca agacaaaaaa
     6901 atatttatat gaacaagtaa aaaacagcga ggccgttatc catgaaatcg gcgaaacagg
     6961 actactcctt tattttgata atcagcaaga agaaacgatt gcttttcgaa cagatatcga
     7021 tgcactgcca gtcaaagaag aaacgaatct ctcttttgct tctaagcatg aaggttatat
     7081 gcatgcatgt ggtcatgacg gacatatggc gatgctttta gggttgactg actttataac
     7141 taaacacatt gaggatctta tttataatgt agtactgatc tatcagccat cagaagaaat
     7201 ttccggtggc gcacaaagcg ttattcagtc aggattgttg gataaatatg aagtacaagc
     7261 aatatttggc tttcatctat ggcctggact agctaaggga gaagttttct ctcgaccagg
     7321 acctttgatg gcacaaagca gtgaaacaga cattatcatt aaaggccagt ctgcacatat
     7381 tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct gtacgatttt tgaagaatgt
     7441 ttatgatttt gaccggcaat taccggcaga gtggaatcat ctaatgaaat ttggccaaat
     7501 ccaaggtgga acgatccgaa atgttttagc tggagaagtt attgtatccg gaagtatccg
     7561 ttcttacagt tcagatatgc aaaattattt gaagagggga ttagaccatt tagcagaaca
     7621 gtttcactct acagctacta tttcttttcg ctataatgac ggctatccag ctgtaatcaa
     7681 ccatagaggc ttatatgcag cactgttaga ttcaaacaaa ctacatgaat tagatgctcc
     7741 agttcttcaa gccgaagatt ttggcatata tacgcaacaa tatccatgtg tatttttctt
     7801 tttaggtgtc ggtgataccc ctgctttaca tgatccgacg ttcgattttg atatggatgt
     7861 tttagaaaat ggtgtagaat ggtacaaaac gattcttttt acagaaaatc ttcttgtaaa
     7921 aatcgatagc agttcaaaag aggagcgagt aaataatgat gaatttatcc aatcgattta
     7981 gtcctaggct ttctagaata gaagtatcaa aaatacgaca attcgatcag caaatatcgt
     8041 cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga ttttccaact cctgaacatg
     8101 tcaagcaagc aggaatagca gcgattgaag aagacttttc tcactatacc ggaatgagag
     8161 gattagagga actgcgggaa gcggcttgta tatttcagca acagcgttat ggattaactt
     8221 atgatccgca aacggaagtt ttgacgacag ttggtgcaac ggaagcgatt gcctctgcgt
     8281 tgttatctgt tttggaagaa ggagacaagg ttcttattcc agcgcctgct tattcaggat
     8341 atcagccatt agtggaacta gctggtgctg aattgatccc gatagatact tctgacacag
     8401 ggtttgtctg tcagccggaa caatttgaac gagcatttga acaatatgga tcagctgtca
     8461 aagcagtcat tttaaactat cctaacaatc ctactgggac gacattgtct gcaaaacaat
     8521 tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt tgttataagt gatgaagtat
     8581 atgctgaatt gacttatagt ggaacgcata tgtccatagc tacttatctt ccagaacaaa
     8641 caatcgtgat aagcgggtta tccaagtcac atgcgatgac tggttggcga gtgggcttta
     8701 tttttgctca aaaagccctt attgacgaac taattaaagt ccatcaatat ctggtgactg
     8761 ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct aataaatggg gtgaaagata
     8821 gcgagaatat gaaagagcaa tatcaaacac gaagagacta tctggtccag caattggcgc
     8881 ctttaggatt tgaagtgacc cagccaaacg gtgcatttta cttgttttgc aaactcccag
     8941 ctgccattca aactgactct tggcagtttt gtgtagaatt agcggaacag gcaaaagttg
     9001 cgtgtatccc aggaattgca tttggcccag aaggagaagg ttatatccgg atttcttatg
     9061 ctagcagcat ggaaaaatta catgaggcct gcaaacggat caaagacttt ttaacagaac
     9121 agtgattcat agattttatc aaaaaaacaa tcgttaattt tgcggtatat tctgcaagga
     9181 gacgattgtt ttttaaattt agttcaaatg aacgcgtttc ttttgattca taactaatgt
     9241 aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat attcaaggag gtttcatcaa
     9301 tggaaaaaat caaagttgtc attgtaggtg catctcatgg agggcatcaa tcgatcttgg
     9361 aactattgac acgatacggt gaaaatgtag atatcacgct atttgaagca ggagactata
     9421 tttccttcat gtcatgtggt atggagctgt atttagaaga caaggtaaca gatgtaaatg
     9481 atgtacgtaa tttcagacca gaaaactttc cgcaatccaa tgttcatatt ttaaataatc
     9541 acgaagtaaa aacgatcgat gccgataaaa aaacagtaac ggttactagc gcatcagata
     9601 acgaaacaaa agaatatcct tatgacaaat tgattttgag ttctggtgtc aaaccaaaca
     9661 atctgccagt accaggagca gatctagaaa atgtctactt gatgcgtgga tacaattggg
     9721 caaccaaaat caaagaaaaa ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg
     9781 cgggttacat tgggatcgaa gcagcagaag caagccgaaa agctgggaaa gaagtcgtat
     9841 tgcttgatgt gatcgataga cctttaggca cttatttaga tgctgagatg acagatattt
     9901 tagaacaaca cctaaatgaa aacggtattg aagttttgac aaatgcaaaa atcgaagctt
     9961 ttactggtaa cggaaaagtt gaggcaatca aaacaagtga aaaagaaatc ccagcagatc
    10021 tagtcattca agctgccggt gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact
    10081 tagatgaacg tggctggatc caaacaaatg aatacttgca aacgaatctt ccagatgtgt
    10141 atgcagtagg agatgctaca cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg
    10201 ctttagcgac tgttgcacgt agagaagcac gttatgtagt aaaacatctt tttgaagaag
    10261 taccaaacaa accgttcggc ggagtcgtag gttcctctgc attaagtgtc tttgactatc
    10321 attttgcagc aagcggattg aatagcttta cagcagcaaa agcaggagta gatgtacgag
    10381 tgtcttatta tgaagatacg ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa
    10441 aagtgtccat ccaattattc tcggatccaa tgacccatca attgctagga ggagcagtat
    10501 tatctaccta tgatgttaca ggtcaaggaa atgtattggc actagcgatc caaaacaaac
    10561 tgactttaga agaactagca gaagctgact tcttcttcca gccaggcttt gaccgacaat
    10621 ggagcatgtt gaatctagcc gctcaacaag cgttaggaga agaaccattt gtagaataag
    10681 aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct tgcatcaagt aataagaaca
    10741 gccaagtaaa aacagcttct tatgttttta acttggctgg acttattacc gcagatgcaa
    10801 gcttttgaac accgtttatt ttcaaaaaag ggactgtgac acgatttgtg taccgacccc
    10861 caaaagttag accaaaaaat ctaacttttg gaggtcggta ttttttatgg ttaaatatac
    10921 ttttgaactt aaattaaaga tcgttcatga ttatttggat ggcaaaggcg attggaccgt
    10981 aaaaatccag atcaagagat tgaagcggaa atgcttaaaa ttcgggagaa gcacaaggat
    11041 tatggctgtt tacggatgac caatgaacta agaaaccgcg gtttctctat caataaaaag
    11101 aaagttcagc gcttgatcaa gaagctagga ataaaagtaa cggcatacac gcgtaaatca
    11161 cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DASNNU010000085        11145 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.085, whole genome shotgun sequence.
ACCESSION   DASNNU010000085 DASNNU010000000
VERSION     DASNNU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.085"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            380..1819
                     /locus_tag="U8116_002182"
     CDS             380..1819
                     /locus_tag="U8116_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN8435145.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2114..2332
                     /locus_tag="U8116_002183"
                     /pseudo
     CDS             2114..2332
                     /locus_tag="U8116_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2581..2796
                     /locus_tag="U8116_002184"
     CDS             2581..2796
                     /locus_tag="U8116_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435146.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3013..3269)
                     /locus_tag="U8116_002185"
                     /pseudo
     CDS             complement(3013..3269)
                     /locus_tag="U8116_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3456..4367)
                     /locus_tag="U8116_002186"
     CDS             complement(3456..4367)
                     /locus_tag="U8116_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN8435147.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4386..5390)
                     /locus_tag="U8116_002187"
     CDS             complement(4386..5390)
                     /locus_tag="U8116_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEN8435148.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5387..7510)
                     /locus_tag="U8116_002188"
     CDS             complement(5387..7510)
                     /locus_tag="U8116_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN8435149.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7515..8435)
                     /locus_tag="U8116_002189"
     CDS             complement(7515..8435)
                     /locus_tag="U8116_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN8435150.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
     gene            complement(8569..10395)
                     /gene="ltrA"
                     /locus_tag="U8116_002190"
     CDS             complement(8569..10395)
                     /gene="ltrA"
                     /locus_tag="U8116_002190"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN8435151.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT         3088 a         2317 c         2031 g         3709 t
ORIGIN      
        1 tataatccta tggaattgaa tatcatagca atgtatttga aaagcaaatt ggaaaacaaa
       61 taggtgcaac accataaata tgaaaaaatt gggaaaaagt agaatggaaa tttgcttacg
      121 tctcacgaga tagctcacac tattaatctg aagaacaatg aagtcgactt ttagaaattg
      181 ctagaagaaa gtaaagcgaa ttatcactag ttactataga gtaagttcaa ataaagcatc
      241 tccaacttac atggttacat gaggatatca aatcgaaatt actcaagctt cctatggcta
      301 gagtcgcgag aagttgttgg gcagtagaca taggttatat tgttcttagc tacacgatca
      361 ttagaaagga aatcctatta tgtatagtta tcaagaagat gcaaaagtcc aaaaaaatcg
      421 ctggtggatt ttgattgcta tatctatgtt taccttcatg tccacattgg atggcagtat
      481 tgtcaatatt gcactaccta ccatctcaga tgatatgggc ataccaatga atcaagcgga
      541 atgggtagtg tccgtttatc tgatgattgt ctgttcttgt ttgttgctgt ttggtaaaat
      601 tggcgatagc cgtgggaaaa tcaaagtatt tcggattggt acgctgatct ttgtcttggg
      661 atcgctgttg tgtgggttca atcattcgct aggtatttta ttgttcgcac gggtggtaca
      721 agccattggt gctagtatga ctatggctac aaatgccgga atcatcacgg aagtctttcc
      781 catacatgaa cgtggtcggg cattgggagc aataggtgcg tttgtttcat taggctcgat
      841 tgctggacca ggtcttggcg gcttgatttt gtcccaattt tcttgggcgt acattttttg
      901 gatcaatgta ccggtgggaa tcgtaacgat gttgattgct gaaaaattct taccaaaaga
      961 tatcaccatg agcggccata caatcgatgg agggggcttt gctgccattg cggctacgat
     1021 tatcagcttt tttggagcaa tctttttggg gcaagaaagt ggctttttcc atccagtccc
     1081 ccttgtactg ttcgtaattt cagtgattgc ctttgtcagc tttttaaaag ttgaaaagaa
     1141 agcagcctct ccattgattt ctttcggtat ttttaagaac aagattttta ccatgagctt
     1201 gattacagcc ttgttgattt tttcttccaa cttctttgtc aatgtagtga ttccttttta
     1261 tctgcaaaat gctcgcgggt tgagtcctag ctatgctggg ctgttgatga tggtctttcc
     1321 tttgatgatg gtcgtcgggg cgcctttaag cggctacctg accgaccggg ttggcccgga
     1381 gattttggta ttagtaggct tgggcttatt gtcggtaacc caggtcatgt atatgttttt
     1441 gtcagtggac tctcctttgt ggctatacat tgtcgctact gccatcatgg gtgccggcaa
     1501 tgccttgttc cagtccccca ataatacgat ggttatgagc tcggtcacta tcgaaaactt
     1561 gggactggca ggctcgatga attcctttgc ccgaaatttg gggatggtca gcgggatttc
     1621 gctttctacg acggtcttat atcaagcgat gtctgctaag atgggccagc aagtaactaa
     1681 ctatatcgca aatcggccgg atgtcttttt atatggaatg aagattacct ttttaggcag
     1741 ctttgttttg tgttttgttg cctttttcct gactatctgg cgtatcaaaa aacaaggagg
     1801 tattcagaaa aatgtctgaa aaaaccactg ttgaagaatt tttggaggcc tgcgtatggg
     1861 tgagtgggat ttccatggat gatggaagat gaagtgaact tctaaaagcc gaaaaactca
     1921 agtattatct tcaaaatact agtgctcaat gagatgcttg atttgatcac aagaagactg
     1981 cgaaagccgc tggcaaccgc aagctaggta tcccaccgat ggactacgtt ttagaggcta
     2041 ctggttatga atacggagcc aacgctctca tctgcataag aatacatcac ccagattatg
     2101 taaagatttt cgatacttct gtcaaaaaat cattaagata gataaatgat ttccgtctag
     2161 tcaaattggt tcagaatgta gacacacaga tggtaagaag tcactcacaa ttcgagaatg
     2221 gacttgtcct atttgccgta tacatcataa tcgaaacgtc aacgatagta tcaatatttt
     2281 gaccaagggc tatgaataca agcattgcct tagaaaacat caagaacctt aggaacaacg
     2341 gggatagctt ggttaataag agataccgct gtttaggctt gcaagtatac tcttttatca
     2401 agaattccgt ggctttagtc atgagaggct taatactatg aaaattttct ttagcatttt
     2461 tgatttaggt tccttttcta attataataa gatgaccact acagtaacct actgaaatat
     2521 ataaacagcc actgtagtac cattttcaaa gtagtctatg agaggagaat cttcattctt
     2581 atgaacgctg gaattgcgga tatgaattta ataaaaaaaa cccttaatga ttttacttcc
     2641 aattcaatat caaaaggtac tggtatcaat ttaagcacaa taaaaaagct gaaaagtggt
     2701 gaaagatcag tggaaaagct gaatctttta gatgctataa aaataacgga atttgctatg
     2761 aaaaatggaa aagctgagat tgaaatatgg agataattat cgtctagtga attacttgcc
     2821 acttagctga atttaacgat ccttggtgct tgaaggggga gattcttggg aatagagcat
     2881 acttgcaagc ctatgtcttt actaacaacg atatctctta ttaaccaagc tatccccgta
     2941 gttctaaggc ttttactttt atctaagcaa taatcgtatt caaaggaaat ttttgaccct
     3001 tagaaagtct cattttatca gacgtgagct taggcattgc tgaattttgt taattcggtt
     3061 ttaggttgtg ctttgagcat aatatgaaag tattctttat cacgatttca ttcatctaag
     3121 gtaatgtgat gtgattctga aattctttca aaggcagttt ttgcataagc tgacattaca
     3181 tcatcaatga catgtttaca atgtttcact actaaaataa gggggtaacc aagaagaaaa
     3241 actgaatgat tatttgtatc taattccatg gtatatcaac catttcctat atagactgat
     3301 tatgacatgg attaaaataa aaaggcacta tgtcttaatt gtcggttttc gagttaccga
     3361 atggcattca tcacccacct atagaggatg ggcgacttct gcctgaaata agttacacta
     3421 cttataactt ctaataaaat tagattgagt cattattatt taacatcaac gattttccag
     3481 tttccatttt tttgtaatac taatttgtac tgaaagtagc tagtggtttt tgatacatta
     3541 tcaaggtatt ttatctttgt actcactaga aaattttttc cgttttgtag ataaactgga
     3601 ttctctaact ccgaaaactt taagtcaata ttgattggtc gcatcgcacc attttcaaca
     3661 taatactcca attctttttc agaagctgat ggataaagtt taaaaaatgt ttctaaaaaa
     3721 tcggtgattt cttccgttgt tttggcatca atttctgaat cgttttctac ttgttttgct
     3781 tcatattttg ccttgtctgg ctttccaatc attgttggca aactagttac taaatgattc
     3841 ctgttttcat cttcaaagat tgtcactcga tatgcagaag aaattgattg cgtctttttt
     3901 ccctctgtaa tatgttgctc cactaaaaat gaaacgacaa attcttccga gtgtttttca
     3961 acatctagaa tttttacaga ttgaacttcg gaactcgtgg gaatatctgc tcgaaccata
     4021 tcttggatta aagcgagacc gttttctgta aggtactgtt cgagattact cattctcttt
     4081 tcaattgctt ctttgttatt tttccatgaa aaatattctt tcgcaaaatc ctctgtaaaa
     4141 ctttcaacgc cagaagtatt cactaccttc tcttttataa ccactttttc atgagttgta
     4201 tgttgatcaa tggcggtgaa atttttataa acgccaaaag caagactaca agacaataaa
     4261 agccaaaggg caagcaccat ttttcgatgc ttacccacgg atacaactcg ttcttttttc
     4321 ttttttgggc tcttttcctt ttgattccgt tcaattttta tcttcattct ttttcctctt
     4381 ttctcttatt tgtatcggcc agctccgata agatgctgcc gccaatagtt ttccgttaga
     4441 ttcgtccacc ccactggatc tcctgcatga aacatgcgat tctcccctgc gtaaatgccc
     4501 acatgggtca cataagtacc ggcatcatag gttgaatgaa agaaaactaa atcacctggt
     4561 ttggcatctt ttagatcaat atgagtcatg gcatcatatt gatcctgagc cacccgagga
     4621 agtttcaggt caactttggc ataacaccat tgggtcaagc cactacaatc aaaccccgtt
     4681 tctggacttg agccaccaaa cacataggcc gttccttcat acttaaatgc ctcatcaaaa
     4741 atttcttgga ctattttgtc atcaaactga gttgtagtta ggtattgctt cactaataaa
     4801 cagtaaaaca tattgccata attataccgc cacccaccat tttccttaat cgcgattggg
     4861 ttggggtaat cgactttttg accacctgat ttttcctttg aaaaagattc agctaaagca
     4921 aaactatgct tcttcccgtt tttcccgaca tgatctagat acccgccacc atagttatag
     4981 gcctgaataa tactatcttg atcacaccct agttctttgg aacgcttaac caaagacgca
     5041 aaatagagac agccttgttt gatggatgcc tcggtactta atgaattagg tggtaatccg
     5101 gctgactcag aactttgcat aacgtcttcc attgtgccac cagattccac ttgaataatc
     5161 gccaaaagaa ttggaatata ctctgttata tccgattctt tggcatactt ttccaccatt
     5221 gcacggtgtt tcaatacttc ttctgagaca ctagttccgc caggagaagt gccagaacta
     5281 ccatttccct cactgtcttc accaaacaac aaacccacac acatcaaaat acccataaaa
     5341 gagatggtca gaaaagttaa tgaaataaca cccactttct tcaagttcat tttttctcac
     5401 ctgaccttga ttttggacga tggttcattc ttttttgtac tttttgattg gtctgctttg
     5461 gcagagtgga cctctgtttc acttgttgga aagaattttt cttacttgct gattggcttt
     5521 tcccttttga aatgatttct tgactagtta gttttcgttt gatttcggga ttcagatttg
     5581 ctctcacaat ccgctttggt gcttcttttt tcagcatcat atctttctga ggttgagctg
     5641 ctttatctat tggaatattc gcccttcttt ttccctgacg atttgttgct tcactcagta
     5701 tcttgcgttt ttcgtccatt ttcgcatttc ttcgttgtcg ttgtttttct cgcaaatcca
     5761 ctcgatccgc tttttcttgg aacagtccgc gcttgaattc ttctggtgct tttttcgact
     5821 tttcgattcc tttatggaga ttgtatttca aattcgttgg cgtattcctt acctgatcct
     5881 ttacttgttt agtcttatct ttcaccaaag cttgggtatc caaaactttg ccgatttttc
     5941 tcccagccaa attcattcgg gattgtttct tattatttga aactggattc tccttagtta
     6001 agtcagatag tatttcgtga tcgttcggta atcgtgaatt ctttcgtaga gaagaaccgg
     6061 atggcttaaa tttggatttt gattgttcct ttgccaactt atttccgact gctgcaccac
     6121 caccaaaagc caaagctcgt cccacatttc gattgagttg ccgcaatttt cgattcatta
     6181 acatttgcgg ttttcgcatg acgcgtcttc ccattgactg gctatcatta ctctggaggt
     6241 tgaacatact cataatgtcg cccaatttga aatagatacc ggcaaaggtc acaatttgca
     6301 gaaaagcaac catgaaaaat ggataggttg ccgaaatatt gaaaaacatc gtggagatgc
     6361 taaatgcagt cgtaatcacc aaagtaacac ccgctcgcat cataatcgta ttgaataaac
     6421 gaataatcgc cttctttcct aaactttcat aagtcggcaa catggacaat aagaaactaa
     6481 ttggcagaaa catcgcaaag ataatgaaga gaatttgcga gaacaacata atccctgtta
     6541 gaagaaaaac aaagaaggaa atgccaatat tgaacaatac caaaaagacc acggtcccta
     6601 aacggttcat ggtcttggta atactgagat tcgcattgtc attgtcttca atctctgcct
     6661 tgaccgcttc ttcccgatct ttgcctttgt tttcatctgg actcacagat agaatcttgt
     6721 tgacccggtc ttcaccaatt tcttctatgt tggaatcatc aaattgcagt agtaaccaag
     6781 gttgttggac ttgaatcgaa aacaaactat cccgaatcaa atccacacta tcttttcctt
     6841 ggctttctga attgggtaca acgatctcgg ttccaagggt aagggccgct tcactaacat
     6901 ctgagctaaa gtcattgatt tttgtgatat aggtaggcgc ataagcaatg aacgaacccg
     6961 atagtagaaa aatcatcaca aaattaatca cggctcgaac tgctttcgtg gtttctcgtt
     7021 ttaataatcc ggtatacgcc acatacaccc ccagagctaa aatcatcaat aatagaaacc
     7081 caaaataaaa tccggaggtc tggagtccat tttcagtaat accagccaat gtttggatat
     7141 tctttccaat actttcagcc gtatccgaaa tgaaatctaa tttgtaagcc tcttgaacca
     7201 cataacccgt ggcgttcgac aaatacaaac tcaccgtcca aatgaaatta gtaatggcat
     7261 aaagaccata catcacgctt ttccctaagc catcacccca attccaaggc aaccaatccc
     7321 aagagctatc cacaaagaaa tctagttgat agttgttcag tgaatatttg gaatatagat
     7381 ttcctgcttc aactgtatca tcaactagcc ctgtcgcctc ggctaccgta ccaactaaac
     7441 tcaatagaac tatcagagtg agtatgctaa taaaaacgat tcccacaatc cgaaaaagtt
     7501 tctttttcat cccctcactc cattctctct tgaacaggtg gacgggtatc aaaggcatgg
     7561 aagagttctt caaagactgg atgtacctga accacgccta cccgaccata caaatcctgc
     7621 atcaagcatt ggccattttc caaatctcgt aggcgttttt gattgctttc atcttctcga
     7681 tccaagccaa agaattccaa ggttttcttg atttcatgaa tatcggtact gcgaaacgca
     7741 aacttcaacc cgatattatt cttcaagcgt tcatccagta aatcatcact attttgcgtg
     7801 acaaagtaaa ccccggcatt catagcccgc ccagcacgaa cgagcttatt cgataatgtt
     7861 ttcccttgcg ctacttgaag aaaactccac gcctcatcta agtctacaat tttgaagatt
     7921 tctcgattac tatggataaa gtccaaggca aaagtggaaa tgacgattag catggaaaca
     7981 ctaagcaatt ccatggtggt atattcttca aaagtggttt ctgcatctgg taaaaccaaa
     8041 tccgctactt gaatgatatt taactgccta tctaaactaa tcgactgggt aatcgaacca
     8101 tctgagaaaa gcaaatgggc aaaatcataa tcggtaaagg actcgatgtg atccgcgata
     8161 ttttccgcaa ctggtgagcc ttcttttcga agctcattaa ttacttgtaa taatccccgt
     8221 tgattgcttt gggtaaccgt ccgaatggcc ttacgcaaca ctggaaactt ctccccatct
     8281 cgactagaaa ttcctgttaa aaaggtcaga atatcaattg ctagactttc agaatccttc
     8341 tttttctcca taatcacata aggatcaagt aatccttgat tttttggctt actagttaag
     8401 ttgagaatat tgatttcatc cgcaatttct ggcaaggttt ctttccagcc accccgttat
     8461 agcgataggt aactttttga tgttatctcc aagttttccc ccgcttcaca ccgtacatga
     8521 aactttcgct tcatacggcg ttccatcata tccaaataga tttataagtt atttcccagt
     8581 tattgtctgg tatccgtaat tcgtccgtat tctgttcagt tttttcttct gctgactatt
     8641 aagaacataa ccttttaaca gtgattttgc ttcgtctgct gattctgtta ggattgcctt
     8701 tgcaatggga atttccacaa atgccagatt agaatatctg tcaaggttat ctcctttgtc
     8761 tgatttgtga atacatatca gagtggacgg agttcctcga cgattcatta catagcagtt
     8821 gccttgttgt gcgatatatg ccgaaatacg attgtcgtta aactctacgc tatcggcagg
     8881 gttttcattt tccaacaacg ctcggatttc ttcttttgtt acaactacaa cgtcctcatg
     8941 caccttgttt ctcccttgct ttgtaaaatt acaaatatct tgcgagaaat tcataggatt
     9001 tttgtgttgt actccttgaa tcggcaatag cggattatcc gcaatcatca ctatttttgt
     9061 ttttggtctg attccttttg tcctgctctg aaattctgaa cttgtacttt caaatggaac
     9121 tgattttgca atgttcctta aacggattcg tatggtcggt aagagagcgt aacttacttc
     9181 tgtcaggttg ttatatacgt tagttgctat gcagtagtaa ttctgcatac cgataactgt
     9241 cagattatat ctgctgatat tgaagccagt cgtatgtctg gctatatctt ttacctttaa
     9301 ctttagattt gttttcgctt tctttaatgc tttttggttc atatctgttt tggcaacata
     9361 accgtgtttc gttcttcctt ttggaatgac tttgattttg aaccccagaa aatctgatga
     9421 atttttcttc agatttacta cctttgattt ctctggactg atttctaatt tcagcctttt
     9481 attaagaaaa tctacggtac tgtggtaaaa tctttgtgct tctccatagg ttgaacacat
     9541 gattttaaaa tcatcagcgt aacgtacaat aaaaccgctt ttcagtttgg tatatttctt
     9601 tgcgtattga agccacccat ttttactgcg gttcttcggc tggaatgttt cccactgact
     9661 gcttacccac cagtctaatt cattaaggac tatgagggag agcagtggac ttaacagacc
     9721 gccttgtggc gttccttttt ctgggacacc ctcgccgtca atttcggctt tcagcatttt
     9781 gctgataatg caaatcaatc ttttatcccg aataccaagt gtccagattt gttttaatag
     9841 ttttccatga ttgacattat cgaaaaatcc tttaatgtct acgtcaacac agtaatgata
     9901 ttttgataaa tttatcagtg tcgttactct gctgactgca tggtgggcac ttcgattcgc
     9961 acggaatcca taggaatgat tatggaactt tggttcgcat atcggctcta ggacttgcag
    10021 gatacattgt tgtaccagcc tgtcccagat acacggaatc cccaatggac gcattttatc
    10081 gctcccagct tttggaatga aaacacgtct gactgatttg gggcggtagt tgctaaggct
    10141 ttcccgaacg gtagcaacaa tctcagcatt gctgagttgt tttatatcgt ctatcgtcat
    10201 tccatccgtt ccagccgttt tactgccact gtttgatttc agattacggt aagctaaaca
    10261 aatattttgt tccgaaccaa taatttcaag taacttataa aagttattac cgttcttgct
    10321 ctgtgcgtat aactcgtcat aaatatgttg catatcataa tactcgttat agcggagctt
    10381 gtttttcttt agcatggtct gtaccctcct tgtttgcagg agcaagtaca tctcttagtc
    10441 ttactcgaac ccataccatt tttcttagta attctattct tcatatgact agtggctatc
    10501 cctccaccgc ttgttatcac ggtttcatag gtactgtgcc accactctca ctgacattaa
    10561 cacaccttat atcattgcta atctcacaat aactttctga tgtgaacact tcacagacag
    10621 taaaatctcc atgttgtcag ctttcaacgt tccaaacaca ctatctgtac atatatcctt
    10681 aggtttcctc tataagcctg ctaccttgat aacgccttta acgttatatg gattttcata
    10741 aatgcggagt cttactcacc cacagtagcg acactgtttg tgtcactgga catttctatc
    10801 cagccccgat atagacctgt acattcaagg attcgtcagt agacctctca tctacattct
    10861 aaccatagat attttataga cttccggcat ataggacaca acgcccacct ctgggaatct
    10921 ttcgtcagca ttatctgtta cggtgtctgt ctgccgactt caccgagctt ctgacagagc
    10981 cttgtttact caagtttctg ccagtcagag tattaaagta gcctttcagg gcgttacccc
    11041 gtcattcgac tattcgtgtg tttagttctc caacacacat taaatattga tttaatgaag
    11101 tgctgtgcct actcttttca agtaggaacg tttcgcaccc ccttt
//
LOCUS       DASNNU010000086        10578 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.086, whole genome shotgun sequence.
ACCESSION   DASNNU010000086 DASNNU010000000
VERSION     DASNNU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.086"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            33..656
                     /locus_tag="U8116_002191"
     CDS             33..656
                     /locus_tag="U8116_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435152.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="U8116_002192"
     CDS             1106..2155
                     /locus_tag="U8116_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN8435153.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="U8116_002193"
     CDS             2152..3033
                     /locus_tag="U8116_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8435154.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="U8116_002194"
     CDS             3036..3806
                     /locus_tag="U8116_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN8435155.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="U8116_002195"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="U8116_002195"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEN8435156.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="U8116_002196"
     CDS             5117..6280
                     /locus_tag="U8116_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN8435157.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="U8116_002197"
     CDS             6397..6807
                     /locus_tag="U8116_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN8435158.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="U8116_002198"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="U8116_002198"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN8435159.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="U8116_002199"
     CDS             9380..9664
                     /locus_tag="U8116_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435160.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="U8116_002200"
     CDS             9824..10060
                     /locus_tag="U8116_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN8435161.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="U8116_002201"
     CDS             10057..10455
                     /locus_tag="U8116_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN8435162.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3691 a         1770 c         2235 g         2882 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttg
//
LOCUS       DASNNU010000087        10567 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.087, whole genome shotgun sequence.
ACCESSION   DASNNU010000087 DASNNU010000000
VERSION     DASNNU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.087"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            30..245
                     /gene="atpE"
                     /locus_tag="U8116_002202"
     CDS             30..245
                     /gene="atpE"
                     /locus_tag="U8116_002202"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEN8435163.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            336..860
                     /gene="atpF"
                     /locus_tag="U8116_002203"
     CDS             336..860
                     /gene="atpF"
                     /locus_tag="U8116_002203"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEN8435164.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            847..1389
                     /locus_tag="U8116_002204"
     CDS             847..1389
                     /locus_tag="U8116_002204"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEN8435165.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            1412..2968
                     /locus_tag="U8116_002205"
     CDS             1412..2968
                     /locus_tag="U8116_002205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEN8435166.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            2982..3884
                     /locus_tag="U8116_002206"
     CDS             2982..3884
                     /locus_tag="U8116_002206"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEN8435167.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            3907..5313
                     /gene="atpD"
                     /locus_tag="U8116_002207"
     CDS             3907..5313
                     /gene="atpD"
                     /locus_tag="U8116_002207"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEN8435168.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            5327..5749
                     /locus_tag="U8116_002208"
     CDS             5327..5749
                     /locus_tag="U8116_002208"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEN8435169.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            6218..6448
                     /locus_tag="U8116_002209"
     CDS             6218..6448
                     /locus_tag="U8116_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEN8435170.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            6545..7846
                     /gene="murA"
                     /locus_tag="U8116_002210"
     CDS             6545..7846
                     /gene="murA"
                     /locus_tag="U8116_002210"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN8435171.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            7859..8035
                     /locus_tag="U8116_002211"
     CDS             7859..8035
                     /locus_tag="U8116_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN8435172.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            8170..8466
                     /gene="yidD"
                     /locus_tag="U8116_002212"
     CDS             8170..8466
                     /gene="yidD"
                     /locus_tag="U8116_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEN8435173.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            8530..8913
                     /locus_tag="U8116_002213"
     CDS             8530..8913
                     /locus_tag="U8116_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8435174.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            9082..9966
                     /locus_tag="U8116_002214"
     CDS             9082..9966
                     /locus_tag="U8116_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN8435175.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            10080..10316
                     /locus_tag="U8116_002215"
     CDS             10080..10316
                     /locus_tag="U8116_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEN8435176.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            10404..10484
                     /locus_tag="U8116_002216"
     tRNA            10404..10484
                     /locus_tag="U8116_002216"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10438..10440,aa:Tyr,seq:gta)
     gene            10491..10562
                     /locus_tag="U8116_002217"
     tRNA            10491..10562
                     /locus_tag="U8116_002217"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10523..10525,aa:Gln,seq:ttg)
BASE COUNT         3499 a         1838 c         2326 g         2904 t
ORIGIN      
        1 tatttatcta ttttaggagg aaaacaatta tgaattatat cgcagcagca atcgcaatca
       61 tgggagcagc aatcggagcc ggttatggta acggtcaagt tatctctaaa acaatcgaat
      121 caatggctcg ccaacctgaa atgtctggtc aattccgtac aacaatgttt atcggggtag
      181 ctctagttga agctgttcct atcttagggg tagttatcgc cttgattcta gtttttgccg
      241 tttaattgaa tcagtattaa ggcagaaaca acatcgtttg ttactgcttt tttacatgga
      301 aactagaaat actccaaaga aaggaaggct cgcctatgct gaatcaattg gcaattgcag
      361 aagttggcaa tgcgatgtta ggtaatatca tcgttgtcag cggctcattt ttgatactac
      421 tggctctctt gaagcacttt gcttggggac caatcagcga tattttgaaa aaacgtgaag
      481 acaagatcgc caatgattta gattctgcag aacaatctcg catcaactca gcgaaaatgg
      541 aacaagaacg cgaacaacaa ttgttagcct ctcgttctga tgcagctgat atcatcaaaa
      601 atgcgaaaga aagtggagaa ttaagccgcc aaaatatttt gaaggatgct caagaagaag
      661 cagctcgtct aaaaagcaaa gcccaagctg atatcactgt agaacgtgat tcagcgctga
      721 actctgtaaa agacgacgtt gcagaactct ctcttcaaat cgcggaaaaa atcttgaaca
      781 aagaattatc tccagaaatg catgaatcat taatcaatca atacattgaa ggtctaggtt
      841 cttcaaatga aactagataa atatacagta ggaagacgtt acgggaaagc tttatttgaa
      901 ctggcgatcg attcaaatca agcagaagaa atctatcaag atctcgtaag tttacgtcaa
      961 atctatgaac aagtacctgg attagggaat atgttaagtg atgttcgtct agaacctcat
     1021 gaaaaacgag aaatcatgga caagcttgtc agcggttatg atggaatcgt taaaaacttt
     1081 ttagaagttg tttatgagta taaccgaatg gatgaccttt tgttcatgat tgaagaatat
     1141 gaacgtcgat atgatgaaca tcaagggtta ctattgggaa gtgtcacaac agcagtccca
     1201 ctttcagacg aacaacgaat acaattagaa aagaatgttg caaaaacaat ggactatcaa
     1261 tcagttgaat taaaacaaat cgttgattct tctattattg gtggcgcgat tgttgaagcc
     1321 aaccatcgag taatcgacgg aagcattcgc acgcaattag aaaaaatgcg taatcaatta
     1381 aacagatagt acaaagaggt aggtgaatcg aatggccatc aaagcagaag aaattagtgc
     1441 cttgattaaa gaacaaattg aaaattatca aaacgtgctt tcagtcgaag aaatcggtac
     1501 cgtaacttat gtaggtgacg ggatcgctcg tgcacatggt ttagaaaacg cgatgagtgg
     1561 tgagttgctt gaattttcaa acggctcata cggaatggcg caaaacttag agtcaaatga
     1621 tgtaggtatc attatccttg gcgatttcga gtccattcgt gaaggagata aagtaaaacg
     1681 aacaggtaag atcatggaag ttccagttgg agaggccttg atcggtcggg tagtcaatcc
     1741 gctaggtcaa ccaatcgacg gactaggtga aatcgttact gacaaagctc gtccagtaga
     1801 agcgatggca ccaggtgtta tgcaacgtaa atctgttaac gaaccaatgc aaacagggct
     1861 aaaagcgatc gacgcccttg tgccaatcgg acgcggacaa cgtgaattag tcatcggtga
     1921 ccgtaaaaca gggaaaactt ctattgcgat tgatacgatc atcaaccaaa aaggtcaaga
     1981 tatgatctgt atctatgtag caatcggaca aaaagattct acagttcgta cacaagttga
     2041 aacattgaaa aaatatggcg caatggatta cacaatcgtt gtgaatgccg gtgcgtctca
     2101 accagcacca ttactttata tcgcaccata tgctggtact gcaatgggtg aagaattcat
     2161 gtacaacggt aaacatgtat tgatcatttt tgatgatctt tcaaaacaag ctgttgctta
     2221 tcgtgaactt tccttgctac ttcgccgtcc accaggtcgt gaagcttatc ctggggatgt
     2281 tttctacttg cactcacgtt tattagaacg tgcagcaaaa ttaagtgatg atctaggcgg
     2341 cggttcaatg actgctttgc catttgttga aacacaagct ggagatatct ctgcttatat
     2401 cccaacaaac gttatctcaa tcactgacgg acaaatcttc ttagaaagcg acttgttcta
     2461 cgcgggtact cgtccagcgg ttgatgccgg actatctgtc tctcgtgtag ggggttctgc
     2521 gcaaatcaaa gcaatgaaaa aagttgctgg gactttacgt ctagacttag caagttatcg
     2581 tgaattagaa gcatttacac aattcggttc tgatttggat gcggcaacac aagcaaaact
     2641 aaatcgtgga cgccgtacag tagaaatctt gaaacaaaaa ctgcatgcac cgctagctgt
     2701 tgagaaacaa gtagtgattt tgtatgcctt gacacatgga ttcttggaca gtatccctgt
     2761 agacagtatt ttagacttcg aacatgaatt attcgaatat ctggatacca atcattcaga
     2821 tatttttgaa acaatccgta caacaaaaga tcttccagaa gaagaaacgt tgaacagtgc
     2881 gattcaggaa tataaagaca tgttcttagc tacaaaagga aataattcct ctacagaaga
     2941 taaattgaaa tccattcaaa atgcatagtg aggtgagatg aatgggtgct tcattaaatg
     3001 aaatcaagac acgtatcgct tcaacgaaaa aaacgagcca aatcacgcgg gcaatgcaga
     3061 tggtgtctgc ttctaagttg acaaaatctg aagcttcttc acagaagttt caggtttatg
     3121 caacaaaagt aagagaaatc gttacgcatt tgactgctac tcagttaaat gatattgcct
     3181 cagaaaatcc tagaggggat atcaattaca acagcatgct gctcagccgt cctgtgaaga
     3241 aaaccggtta tatcgtcatc actgcagatg gcggtctggt cggtggatat aacagttcga
     3301 tattgaaaca aacgatgtct attttagaag aagaccacca atcacccgat gattacgtga
     3361 tgatcgcaat tggcggaact ggtgcagact tctttaaagc acgcggtatc aacctagcat
     3421 atgagctgcg caatttatct gatcagccaa gctttgatga agtccgcaaa atcgtaaata
     3481 tggcaacaac tatgtaccaa aacgaagtat tcgatgaatt atacgtatgt tataaccatc
     3541 atatcaattc gctaactagt cagtttcgtg tagaaaaaat gctgccaatc tcagacttag
     3601 atccagaaga agcaacgact ttcgaacaag aatatatttt tgaaccttca aaagaagaaa
     3661 tcttgtctca gttattgcct cagtatgctg aaagtttgat ctacggtgcg atcgttgacg
     3721 ccaaaacagc agaacatgct gctggtatga cagcaatgaa aacagcaact gataatgctg
     3781 ctaatatcat tgatgagctg acggtttctt ataaccgtgc aagacaaggg gctattaccc
     3841 aagaaattac cgaaattgtt gccggagctt cggcactaga atagtattga gattggagga
     3901 aaaaagatga gttcaggcaa gattgttcaa gtaatcggtc ccgttgtcga cgtggaattt
     3961 tctttagacc aatccttacc agatatcaac aacgcattag ttgtttataa aaatgacgaa
     4021 aataaatcaa aagttgttct tgaagcagcg ttagaattag gggacggagt gatccggacc
     4081 attgcgatgg aatcaacaga tggtttacaa agaggaatgg aagtcattga tacaggcaaa
     4141 gcaatctctg ttcctgtagg taaagaaaca ttaggtcgcg tattcaacgt actaggagat
     4201 acgatcgatt tagaaacacc tttcccagaa gatgcggaaa gaagcgaaat tcataaaaaa
     4261 gcaccagcct ttgacgaatt aagtacaagt acagaaattt tggaaacagg gatcaaagtt
     4321 atcgatttgc ttgccccata tttaaaaggt gggaaagtcg gactattcgg tggtgccggt
     4381 gttggtaaaa ccgtactgat ccaagaactg atccataata tcgcccaaga acatggtggt
     4441 atttctgtat ttaccggtgt aggtgaacgt actcgtgaag gtaatgactt gtattatgaa
     4501 atgaaagatt caggagttat cgaaaaaaca gccatggtgt tcggacaaat gaacgaacca
     4561 ccaggtgcac gtatgcgtgt tgctttgact ggtttgacga ttgcggaata tttccgtgat
     4621 gtagaaggtc aagatgtact gttgtttatc gacaacattt tccgtttcac tcaagctgga
     4681 tctgaagtat cagccttgtt aggacggatg ccttctgcgg ttggttatca accaacattg
     4741 gcaacagaaa tgggtcaatt gcaagaacgt atcacatcta cgaaaaaagg ttcgatcaca
     4801 tcgatccaag caatctatgt gccagccgat gactacactg acccggcgcc ggcaacagca
     4861 ttcgcccatt tggatgcgac aaccaacttg gaacgtaaat taacagaaca agggatttat
     4921 ccagcggtag atccattggc ttcttcttca agtgctttag cacctgaaat cgtgggagaa
     4981 gaacattata aagtagcaac agaagtccaa cacgtattgc aacgatatcg tgaattacaa
     5041 gatatcattg ctatcttagg tatggatgaa ttatccgatc aagaaaaagt gttagtttct
     5101 cgtgcccgca gagtccaatt cttcttgtca caaaacttta acgtggctga acaatttact
     5161 ggtcttccag gttcatatgt ccctgtagaa gaaacagtga aaggattccg cgaaatcttg
     5221 gaaggcaaat acgatgatct tcctgaagaa gctttccgaa gtgttggccg aatcgaagac
     5281 gtcgtagaaa aagcgaaaac actaggctac tagaaagggg tgccccatgg atcaatattt
     5341 aacagtaaat gtggtaactc ctgccggttt ggtctatgat caccatgcag ctatcgtcgt
     5401 tgcacgtacg acagctggtg agcttgggat tttgcctaaa catgcaccaa tcatcgttcc
     5461 attgacgatt gatgaagttc gtgtaaaacg aacagattca gatacccacg tcgattggat
     5521 cgctgttaat ggcggaatca tggaagttcg tgataatgtt gtttcaatcg ttgctgatag
     5581 tgcggaacgt gaacgtgata tcgatgtatc tcgtgcggaa cgtgcaaaac aacgcgcaga
     5641 acgccaaatc gaagaagcaa aagcaaaaca agatacgaac gaattgaaac gggcaactgt
     5701 tgcgttgcac cgagcaatca atcgtatcaa agtatctaag cactcataaa atgagatgcg
     5761 gatgtttatc cgtgtttaag aacagtaaaa agaggttatg acagaagtgg tcagcttcaa
     5821 gacataagaa ggaatttacg aaaattgctt ctcaaatttt tgtgaaatct cagcttattt
     5881 ttcgaagaag ctacttctgg aacaccgtat atcagaggtt gtgaaatcag cgttttgtcc
     5941 tgagtattag agaaacaaat ggaaaatagt tcctcatatt ttgccgtttt ttgagctaat
     6001 accgagggtc agatgattga acaccgttta tttggaataa gggactgcga caagactttt
     6061 gtctcagtcc ctttttttgc atatttttag catttatttc ctaaaaaatg taaggggatt
     6121 cctatatttt taagtatact tgtttttcct tttatacatt ttatggtatt atcattttat
     6181 tagttattat aaaacaatcg ttagaaagga cgtcattatg caagtatacg gaattgatgc
     6241 ggtgcttcga atcgtgagtc atttggcgtt tatctactta gccttctggt ctttacgttc
     6301 gttacgcatt gaaacttttt tcagaccaat gcataatacg caaattagaa tggcaatcgt
     6361 acttttttca atttttttag ggtacactgc aagtaatttt ttcttagaag ttatcgcttt
     6421 atgccgaaat cttttcgtta gcctgtaaat tttaaaaaaa ggtactattt taaaagcaag
     6481 gatgttataa tagaaaagat tttgagtaag tgtatgctca ttggaatttt ttggagggaa
     6541 caacatggaa gagatcatcg taagaggtgg caatcaatta aatggaacag taagaattga
     6601 aggagctaaa aatgctgttt tgcctatttt agctgctagt ttgttagcag aagaaggtat
     6661 tacgacatta gacaacgttc cgattctttc tgatgtcttc actatgaatc aagtgattcg
     6721 tcacttaaac gtagatgtag attttgatga acaaaagaat caagtaacaa tcgacgcttc
     6781 aagacaatta gaaatcgaag cgccttatga gtatgttagt caaatgcgtg cttcaatcgt
     6841 tgtgatgggt ccgctacttg ccagaaatgg acatgcaaaa gtagctatgc ctggtggatg
     6901 tgcgattggt aaacgaccaa tcgatttgca tttaaaagga tttcaagctt taggagcaaa
     6961 aattattcaa aaaaacggct atatcgaagc aattgcggat gaattgatcg gtaacacgat
     7021 ttatttagat ttccctagtg tgggtgctac tcaaaatatc atgatggctg ccgtaaaagc
     7081 aaaaggaaca accatcattg aaaacgtagc tcgcgaacca gaaatcgttg atttagcaaa
     7141 cattttgaat aaaatgggcg ctcaagtcta tggagctggt actgaaacaa tgcgtattga
     7201 aggcgtggat catttacatg cagtcaacca ctcaatcgta caagatcgta tcgaagcagg
     7261 tacctttatg gttgctgctg caatgacaca aggcaatgtg ctgattgcag atgctatctc
     7321 tgaacataac tgcccattga tttctaaatt gatcgaaatg ggtgcagaaa tcattgaaga
     7381 agaaggcgga gtccgtgtca tcggaccaaa acatatctta cctacagatg taaaaacgat
     7441 gcctcatcct ggtttcccta cagatatgca agctcaaatg acagctatcc aattagttgc
     7501 agaaggtaca agtgtggtga ctgaaacagt gtttgaaaat cgtttccaac atttagaaga
     7561 gatgcgccgt atgaacgctc atgtcaaaat cgacggaaat gtagcaatca tggatggaaa
     7621 ccatgaattg caaggtgcag aagtatatgc aacagacttg cgggcagcag cagcattagt
     7681 tttagctggc ttgaaagcaa atggaatcac acgtgtgcgt aacttgaatt atctagaccg
     7741 tggatattat aatttccata tcaaactaca gcaattaggt gcagacgtcg aacgtgtaga
     7801 catggatcaa acttctgctg aaaaaacagc acaaacgatt gcttaaggaa gtggctgaat
     7861 gagtaaccaa cgatatatct tattaacttt gataaagatt ttagtggtga tcttattgct
     7921 tatccttctt tttgtggccg gaacgatgat cggatatggt gtcatcggtg gcggcaatcc
     7981 atttaaagta ttccagccaa gtttgtggat tcatattcga gacttttttc attgataaat
     8041 aagaatcaca gtggttctag tcgatatgaa gtgagccatg aagcagaaca tgataaaaga
     8101 aaacagaagg gaacgttgac gttagtcgcg atcccttttt ttagtaaaag aaaagaagga
     8161 atctaattga tgaagaagat attgatttat ttagttagag gatatcagcg ctttatttcg
     8221 cctgcatttc cacctagctg ccgttattac ccaacctgtt ccaattatat gataaaagca
     8281 attcaaaccc atggtgcgct aaaagggacg gtaatgggga ctgcacggat cttacgctgt
     8341 catccttttg tcaaaggcgg tatcgattat gtaccattga cattccgatt aacgcgaaat
     8401 aagaaagata aaaaagaacc agattataaa aaggttgtga aaaatacggg tagcactgag
     8461 cgataaagaa aaagaggaaa tttattgtca aagtaacaac tccgcaacta cttatatggg
     8521 ggacaaaaaa tgatcgaaga actaaacaag aaagatttac catggtcctt gttattagat
     8581 gcagatcctg ataaagaaaa agttcaagta tatgtttcca gagggagtgg ctggatatgg
     8641 aaagaaaaag aaaaaacaat aggtgtactg atctatgtgg caagggaaaa agaatacgaa
     8701 atcgtcaatg tagcagtaac cccaacgtat caaggaaagg ggattggcgg aaaattactt
     8761 gagacagctt ttcagaagct ttcacagcta gcttcaaacc aaactcggat cattatacgt
     8821 actggcacta ctagtagcgc tgcgttacac ctctatcaaa aaatgggcta tgtggagatt
     8881 ggtcgagtca aagactattt tatccatcat taatctgagc ccatttttga aaatggagat
     8941 caattaaggg atcaagtgat tttagcaatt cacttaccta aaaatagctt taactaataa
     9001 actttcaatt cgagaaaatg ctttcaaatg ctaaatagat acattacaat agctttagca
     9061 aacaaaaagg agaatatttt tatgggaata ttagtttttg attttggtgg atcggctgtc
     9121 aaatatggtt gttgggatgg caaagagatt aaaggtaagg gacaatttgc aacgcctgaa
     9181 tcttgggaag aaatgaaaac gcaattattt caagtttata aaaaaatact cgtttctttt
     9241 gaagtcgaag gaattggaat cagctctcct ggagtagtga ataatgagaa acaggtgatt
     9301 gagggaatca gtgcgattcc ttatattcat ggatttaata ttttccgaga tttggaaact
     9361 ctgtttcaac tcccagtaac gattgaaaat gacgccaact gtgccggaat ggctgaattt
     9421 tatgaaggtg ctgcccgtga ttatcaagat gttgcttttg ttgtggcagg aactgggatt
     9481 ggcggggcgt tgttcacgaa aggacaaata aataaagggg cacatcttta cggtggagaa
     9541 tttgggttga tgttcttaga tggcgataag acattcagta aacttggtac agcggtacag
     9601 atggcttggc gttattgtga acgcaaaggt ctcgataaaa atgcttatac cggaaaagat
     9661 gtctttgaat tagcagaaac tggagatgca atagcaaaag aagaagttga gagtttttat
     9721 acgtatctta caaagggatt attcagtatt caattttcgt ttgatccaga agtgatcgta
     9781 ttaggcggcg gggtttctgc aaaagaaggg ctcattgatg agatcaaaag ccgaatgaag
     9841 aagctgacag aacaattcga tctgcatgat ttcgaaccgc agattttatt atgtgagtac
     9901 agaaacgatg ctaatcttgt aggtgcagca gcaaactatc tcgctgtacg tcaaggagag
     9961 aattaagagc tttagagaag gtgcagcttg cataaaagaa gagaatgaaa tgaataggag
    10021 atttttacct aaggaatgtt ttatttgctg cagtatgata aaatatctga gaggtgaaaa
    10081 tgatgtcaaa aaaaaataaa gatattgaag tgcgtataga agaagtgaaa aaagatatca
    10141 aaggtaagaa ttatgaagtc actcagttat ttatagggaa aaaaatgatt ggtgagatct
    10201 tagcctatgg gccaaaagaa tatgaaattt tctttggtga agaagatttt ggaaaagaaa
    10261 aaagtcttga aaatgcgata gaaacagtga ttcgacactg gaatctgcac gaataaaaaa
    10321 atatcaaaaa aactttcaat aaagggttgc aaaaatctgg aaaattaggt attataacta
    10381 agtcaggttg atacaaacct ttcggaggag tagcgaagtg gctaaacgcg acggactgta
    10441 aatccgttcc ttagggttca gtggttcgaa tccactctcc tccatttcat tggggtatag
    10501 ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc cagctacccc
    10561 agtatac
//
LOCUS       DASNNU010000088        10543 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.088, whole genome shotgun sequence.
ACCESSION   DASNNU010000088 DASNNU010000000
VERSION     DASNNU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.088"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            106..807
                     /locus_tag="U8116_002218"
     CDS             106..807
                     /locus_tag="U8116_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN8435177.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1416..2432)
                     /gene="tsaD"
                     /locus_tag="U8116_002219"
     CDS             complement(1416..2432)
                     /gene="tsaD"
                     /locus_tag="U8116_002219"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEN8435178.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2429..2893)
                     /gene="rimI"
                     /locus_tag="U8116_002220"
     CDS             complement(2429..2893)
                     /gene="rimI"
                     /locus_tag="U8116_002220"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN8435179.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2900..3442)
                     /gene="rimI"
                     /locus_tag="U8116_002221"
     CDS             complement(2900..3442)
                     /gene="rimI"
                     /locus_tag="U8116_002221"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN8435180.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3426..4148)
                     /gene="tsaB"
                     /locus_tag="U8116_002222"
     CDS             complement(3426..4148)
                     /gene="tsaB"
                     /locus_tag="U8116_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEN8435181.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4339..5319)
                     /locus_tag="U8116_002223"
     CDS             complement(4339..5319)
                     /locus_tag="U8116_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN8435182.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5343..6827)
                     /gene="galT"
                     /locus_tag="U8116_002224"
     CDS             complement(5343..6827)
                     /gene="galT"
                     /locus_tag="U8116_002224"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEN8435183.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6839..7828)
                     /gene="galE"
                     /locus_tag="U8116_002225"
     CDS             complement(6839..7828)
                     /gene="galE"
                     /locus_tag="U8116_002225"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEN8435184.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8076..8243
                     /locus_tag="U8116_002226"
     CDS             8076..8243
                     /locus_tag="U8116_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEN8435185.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8305..10116)
                     /locus_tag="U8116_002227"
     CDS             complement(8305..10116)
                     /locus_tag="U8116_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEN8435186.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10113..10478)
                     /locus_tag="U8116_002228"
     CDS             complement(10113..10478)
                     /locus_tag="U8116_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEN8435187.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
BASE COUNT         3001 a         2226 c         1854 g         3462 t
ORIGIN      
        1 tgtaaagtat taaaacttgt ttctaatgtt ggtatgttaa aactttacaa attctttaaa
       61 aaaagtgaca ataaatatag tgaacaataa ggagaggact ttttaatgcg tgttaataaa
      121 atcgattttc acttttcaaa actcagatat caacctgatt ttgaacattt ctcggatgaa
      181 gagttccaac agatcaagga acatactgtt cttcgttctt ataaaaaagg acagatcctt
      241 ttcgacgatg gtgatcccag acaacggttg tatatcttga ccaaaggact cgttcgttta
      301 gaacgttatg atgaaagtgc aacttattac tactatgact atgtgaatac aaataacctt
      361 tttcctatgt caggaatgtt tggagaagag acctacactt atacagctca agcgatgact
      421 gatattgaaa cattctacct tcctgtttcc tactatgaaa aattagcacg aaaaaatccc
      481 gaacagttga tctatatgat acaaaaaatt tctaaagtag ttgaaataca tgaattgcgt
      541 atccaaatcg gattgacctc tagcgccttt gaccgggtaa aacaaaattt attcattttg
      601 aaagaagaat tgggcgtacc aacaaaagat ggtcatatca ccattcctta tccgatcacc
      661 ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca agtaatcaaa
      721 caactacgtg attccgggct gttagattat gacaaaaagc gttttacttt ctgtaagaca
      781 gctactactc cagctatgag agaataacgc gaacaaaaat aagcagtgac cctgataaat
      841 atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca gttttagatc
      901 actggacaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt cactgtttgc
      961 aaaaaatact gttagattaa ctagatgaag cgtcttgtcc tcatccgatc tagattattc
     1021 gatattcctc gacaataaac gattactcag tgagggcaat cagaggaacg gataatgata
     1081 aataagctgt aaaaaatagc aaaataacaa taatgattaa aagtatgtaa aaaatagggc
     1141 tttgacaaaa acgtgtcaca gccctatttc gattaaacgg tgtgtacctg tcaactcttc
     1201 ggtcacaagt cataatgttc ataaacatga ggagctgttt tttgaaattg ttccttgtga
     1261 atcagagtta aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa agcttgcata
     1321 cgcggtaata agaacagcca ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt
     1381 attacttgat gcagggcagc tttttcacaa cctctttatt cagataaaac caagcttgga
     1441 tcagcattca actgatagct agcaaaattt cttttttgca tttccacatg tgctgctgct
     1501 ccaatcattg cagcattatc tccacaaagt cgaagagggg gaatcactag ttctacctca
     1561 gggagtttag ttgccaaagc atcctgcaat ccttcacgca acccttgatt ggccgctaca
     1621 cctccagcta taatcagttg cttgactgga taattttgac aagctcgaag tgttttagta
     1681 atcaatacat cgatcacact tgcttgaaag ctagctgcta aatcattttt gtctagatct
     1741 tctccacgct gttgtgcatt atgcaccaaa ttgataaatg cactttttaa tccgctaaaa
     1801 ctgaaatcat aattatcttc atggatcatc gctcgcggaa aatggtaatt atcttttccc
     1861 tgatgagcta gctgatcgat ttcttttccg cttgggtaag aaagacctaa tactcttcca
     1921 actttgtcat aagcttctcc tgctgcgtca tctcttgtct ccccaatgat ttcatatgat
     1981 ccatcttctt gcatatagac taattctgta tgtcctccgc taacaagaag tgccatcaat
     2041 ggaaactgga atggtttaac cagacgggct gcatagatat gccctgccat atggtttaca
     2101 ggaataagcg gcaattggtg tgcccaagca aaagccttag ctgcagaaat accaatcagc
     2161 aacgagccga caagtcctgg tccataagtg acagcgactg ccgataaatc ttctgccgac
     2221 acaccagctt ctaccagtgc atcttccaag cataaggtga tttgttctac gtgatggcgg
     2281 ctagctactt caggtactac gccaccaaaa cgtttatgac tgtttatttg agaagcaacg
     2341 atgttagata agatctctgt tccatttcta acgaccgcta cactcgtttc atcacaactt
     2401 gattcaatcg ccagaatcaa ttcttcattc atttcatcgt ctccgttttt aactttctgc
     2461 tcataatgat cgcatcttcc gtcggattgc gataatatga tttccgttta cctaatatac
     2521 gaaacttttc tgattcataa agttttcttg ctgcttcatt agatagacgt acttctaaaa
     2581 aaacatgcag cgaatctttt atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat
     2641 gcccttttcc ttgttctgaa actgcaacag caatatttgt gatctcgact tcatctatta
     2701 ctttcgagta tccaataaag ccacagatct ggttatcttc agctaaaagc agataatcag
     2761 agtgaggctg ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat
     2821 aagcattttc gctaatcatc catacgcatt ctgccaagta cgcttcatca aaatcttttt
     2881 ttctgcaaat caactaatct cactcatttt ctaaaagttt tatcatatcc aacgcatctt
     2941 cttttgtttc agtgtaatat ctttttttga tcgttgttgc ttgaaacccc aatttccgat
     3001 aaagtctttg tgcatcttga ttactcatac gcacttctaa tgacatcgtc tcacagcgat
     3061 tcattatagc aaagttctcg atttcgtcaa taagcagcga accgattcgt ttacgctgat
     3121 atgccggtcg aacggcaata ttcgtgatat ggcaatctaa accgaacgct cttgaaccga
     3181 taaaaccaat cattcttcca ttatcaaaga tacctagata tagatgagga agcggtgact
     3241 tcaattctga tataaaggca ctttttgtcc atggcaattc gccattatag acttctcgtt
     3301 cgacatccag cagctctttg atatcttctg tagtcaattc tcttagtacg taacgacgat
     3361 cgatttgttc gactgttttt tctgtataaa tcattttatc ttgaaaaaag cttctggcat
     3421 acgctctaaa ttttttcaac ataactttcg tctccaggtg tgtgcgtctc caaccatttt
     3481 tcttctgctt ctactcgttt taaatagcga ggaagaaaag cttgtttctc tgcaactggt
     3541 tcaactaaag cacctaattg agccacagtt acaccacttg gaatatccca tgctggaact
     3601 gtattaatca tcgcactagg caatatttcc tttatttcag agaagaattt gtgacaatca
     3661 ctcccgacga aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca
     3721 gaaatatgta catcttcaag tacagtttct aatctatttt ctttccagcg gtatgcccca
     3781 gcgtatacat tttttcttcg agcatcaaac atgggcacaa taatattatc cacaccgatg
     3841 caattagccg cgatggtttt cagactagat actcctacta gctctttatc taatgtataa
     3901 gcaatcgttt ttgctgtcgt tacacctata cgaagccctg tataagaacc gggaccatca
     3961 gaaaccgcga ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa caattggtcg
     4021 attgcaggca tcagtgttac actgtggttg cgtttgatat ttgtttgtat ttgtccaaga
     4081 atttgttcgt cttctaccac accgatcgcc aaagtttggt ttgcagtatc tattcctaat
     4141 gtaatcatat atttgatcct ttctgctatg acattctcgc cttccattgt agcatacttt
     4201 tttattctga ggatgaaaat accaaaggca aaactagtac aataatacat gattatctgc
     4261 tggcgggcaa attttgcccc ttcctgaaac acaaaaaagc tgcggtatca ctccattttc
     4321 ccacagcttt tccatctttt atttcgttga ttctcgaata atcaactctg tttccacaat
     4381 aatctttctt gaaaccggtg ctttggaatt acttaattga agaatcgtct ccactgctag
     4441 ctctcccatc cattcggtat aaacttttac agtagaaagc gagggcgtta catatttcgc
     4501 tacactgaca tcattgaagc ccatcacaga aatatcttct ggtaccacta aacctttttc
     4561 ttgaatcgct ttcagtgcgc caatcgctaa tgcatcatta gctgcaaaaa gcgcatcagg
     4621 aacatgcgga ttcgttttta aatactgctt cactgcttga tatcctgact cgacagaaaa
     4681 atctgcttcg ataattggac cttcgtcgta gcctttttct gccaaaactt gttgaaatgc
     4741 cataattctg ggatctttta gccgctgatg gtttttcttc gtatattctg ttccagcaag
     4801 catagcgata tgttgcgctt tatgggagac aaagtgttca atgacttttt gaacacttga
     4861 cttaaagtcg acaactagag agtcgattcc ttgagaagtg ccgtcagaat ctaccaaaag
     4921 caaggcattt tgggatcctt tcatttctaa taactgagct tcgtcgaatt ttcctagtgc
     4981 gatcgttcca tctgccggaa gatcactaat ggaatcccac gtttctttca ataaagtaac
     5041 gtttgcttct tctgcctttt tttcgattcc taggcgaata gataaataat aaagatcttc
     5101 aagctcttcc gtatcgtcgt accactggat caggcgaagc gtttgcttag tatttgctgc
     5161 atttttacga tgtttcgtat aatttaagcg ttctgctgct tcaaaaattt tttgctttgt
     5221 ggctaagcca acggaaagat caggatcata gttgagaact cttgaaacag tagcaggaga
     5281 aactcctgcc aactgcgcaa tgtctttaat tgttgccatt tgtctctcct ccttttttac
     5341 gtttataaaa tcgcagtaaa tcgttcaaac gctgcctgtc ctttttcatc tcgcttgaat
     5401 actccagcgt cttccagtac acgtgcaaaa acctgaccta cagatttttg tacgacatcg
     5461 gtgaccgtat ctttcgtcaa atcaggatgt tcttttttta attgttctgc ccacggacga
     5521 tgatattcag ccatctggtt ttcttctcct aataagaaat gctctacttc tactagttct
     5581 ggtttcaaac gaggtggtaa aacagcaagc cccattactt cgatcaaccc aatgttctcc
     5641 tttttgatat gttggacatc tttatgtgga tgaaaaatcc catcaggatg ttcttctgaa
     5701 acattgttgt ctcgaaggac aaggtccaac tcaaagtaat tgtcccttct tcgtgcaatt
     5761 ggcgtaatcg tatggtgtgg tgtaccatct gctgaaaaag ctctgataga tactgtttca
     5821 tccgaatatg ctttccattt ttccaatact gagttagctg cttcaataag caagtttttc
     5881 tctgttgatt gcaaacggat cacagacatc ggccatttca cgactcctgc gatgacttgc
     5941 ggataatctt ctagtttgat aagtttttct atcggtgctt ttgccatagg aaattcatga
     6001 cgtcctgcct gataatgatc atgagaaaga atcgatcctc caacaattgg taagtcagca
     6061 ttcgaaccaa caaagtaatg aggcaagact tcagtaatcc gaagcagtct agcaaatgtt
     6121 tcctttgtga ttttcatcgg gcggtgttgt tctgataaaa tgatcgaatg ttcattgtaa
     6181 taagcataag gggaatattg gaatccccag ctttcaccgt ctagattcat tcgaataatc
     6241 cggtgattgg tgcgggctgg atagttcaag cgacctctat atccttcgtt ttccatgcaa
     6301 agcatgcagg taggatagtc tacttttacc gcctcgcgtt ctgctgcgat ttgttttgga
     6361 tctttttctg gcttagataa attgattgtg atttccagtt caccatactc cgactctgcc
     6421 ggaaatttga tgttttttgc gatggctctt gttttgatgt agtcattttt cttacttaaa
     6481 ttaaagaaat actcagttgc ttcctctggg cttttcgaat agtgctgtgc aaagaatgca
     6541 ttgacgactg aaggtggcgg tgtgagaaaa tccattaatt gcgcttcaaa aatctcttgt
     6601 tctgctggca attctgaaag aacaccattt tctcttgcct tagcgacaag ctgatccaat
     6661 aagtctacag attcttcctt aactggtctt acttcaatgc tttctagagc atcttctcct
     6721 accatcccta aaagacgatt ctgtaaatat aagcggtcca tttccatcca tccgccagct
     6781 tcgatcgcaa gtgtggcaaa atcacaaatc gtttgactga ttgacatatc tcattcccct
     6841 attcttcata accatttgga tggctgacat gccaatccca tgctgttttg ataatcgctt
     6901 tgatatctgt gtattcaggt ttccagccca gaatctctct cgctttttca cttgaagcga
     6961 ctaatcggct aggatctcca gctcttcttg gtgctatttc tgctggaatt tcttttcctg
     7021 tcacttctct agctgcttct agcatttcct tgactgagta tcctttgctg ctgcctaaat
     7081 tgaagaagtt gctttcattt ccttctttca agtattccaa agctaaaata tgtgcagcaa
     7141 tcaaatcttc tacttgaaca taatctctga tacatgttcc atctggtgta tcataatcgt
     7201 ctccataaac agccaaggct tttctttgac caagagctac ttgtaaaatg attgggacaa
     7261 gatgcgtttc tggtgtatga tcttctccga tagatgcatc tgcttttgct ccggctacat
     7321 tgaagtagcg caaagcgaca tagcgcatgc catatgcttg gtcacaccac ttcatcattt
     7381 tttccatcat caatttactt tcaccatacg gatttttagg attagctggt gtatcttctg
     7441 tgattggaga ctctttcggc tctccataag ttgctgcagt agaagagaat acgattttat
     7501 tcacgttgaa ctcgtgcatg acttcaagta agatttgcat accgtatacg ttgttgttga
     7561 aatacatcaa tggtttttcg acagattctc ctactaaaga gcttgcagca aaatggatta
     7621 ctccttcaat cggttccttt tcaaatacgg aacgtagaaa ttctttatcc cggatgtctc
     7681 cttcataaaa atgagcatct ggatgtactg cttgtttgtg acctgtgagc aaattatcta
     7741 ccacagctac ttgatacccc ttttgaacta gctggtctac agcgtgcgaa ccgatataac
     7801 cggctccgcc tagtactaaa attgtcataa aaaacactcc tttgattagt aaaattgtgt
     7861 ttgttgattc tatataattt aaaaaacgaa gtttactttt atggtgaaaa caaacaaagt
     7921 aaaatttcgt aaattcatat ggtaaattta taataaaaca tttagtaaac attttcaagg
     7981 attttttgca aaggtttaca aacaaacgta aaactagcct ttttttctct tttgttccat
     8041 aatagatata acaaataata aatgaggtgg attcaatgaa aaaatttgta tctggaatat
     8101 tagtaggaag tctggcaaca gccgctgccg ttgctggttt ggttgcttct gtgaaaaaaa
     8161 cagtgatcga tccaattgac gaaaaagaag caatgatcga agaaaaccgt aaaaaagcaa
     8221 tgcgtaaacg cgtttctcgt taataattat ttaaaaatac gcatgaagac tttctaaaaa
     8281 acagataagt catcatgcgt atttttattt gtcagtcaat ataagttgtg ctattttatc
     8341 tgcctgtctc agattccatg gatctgtata aggtctagct actgctgcag ttagccaatc
     8401 cacaccttca acaggtattc catagcaata aaagtcagac tggatttccc ctgaagcatc
     8461 aatcacgcga ttcgtacctc gctcaacatc tatagcttct gaatgataac ttcctgtatt
     8521 atctgaaaat gaaaaagggc gaacaagttt gttttccttc aaatttttta tgacaggatt
     8581 acttgaacga gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc gactgctgat
     8641 ttctttatct tgtagagaaa taacatagcg gttgtgcttt tgctgaatat caaatggcgg
     8701 ttcgatcaaa tgaaaaatac ccgcatctat cagtgctgct aattctttcg tccgaatgat
     8761 tggcggaccg atcgttaaaa agctatttag atgagtaaac catccccata gtatttcttt
     8821 gtattcctta gcagaaaaaa cgtgccattc aatagcctgc cggattggat cacgccagtc
     8881 ttttaatgca tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt ccgcttcttt
     8941 tatctgccct tcaataaata ttcggctagc ttctgaaaag ggctcattct gataggcaag
     9001 aggagtttct aaatacgacc aatcccatct ataagggatt agtgctggat gctttccgag
     9061 acactcctct gaagagaggg agagaaaatc atgctcaaaa tttttctttg gaatcggcaa
     9121 gtgatattct tcgatgattt tcttgtaata aaacaactca acgtcttttt tcagatagtc
     9181 aaaaaatgtt tcgccagtca aaaatcccga ctgatgccat tgctccaggt tttcatttgt
     9241 tagtaaccgt ggccatgcca ttgctccgcc ttgcttttga tttcttcctc gagggtaata
     9301 agggagcccc cttcttgatc cactgtatac aatcggttcc ttgcctgatg gatgatagac
     9361 cagttttccc ttttcgttct cgaagcgccc tcctcttgat actgtaaaca atccgacata
     9421 atcaaaaaaa cttaagccta gacctcttaa aaagacattt tctttaggcg gtatcttcga
     9481 aagatctaca tctgcaggat tagaaggaag ctgatacagt aaaacatttt tctttgcgta
     9541 gtctacccac actctttctt cttctgtctc atcattttcc cagtgaccgc ttgctaaaac
     9601 aagctgatcg atagaatagc tttgctgatc tgtctgtaaa agataaccat cttttctttt
     9661 gcttactttt tttactaaag aataaacaaa ctgaatagaa gctggaaatt cttcttgcaa
     9721 ctttgagaaa aaccagcgtt gatagagtcc gtataaacat cgagtagatt gctgatcttt
     9781 ttccagacga ttgatttctt ctataattag ttgcttattt ttggttaccg tctgttcgat
     9841 ataatctgta gcaggtcctt tactccattg aaacaagttg ggtccctgtg ttaccaatcc
     9901 tccactggac aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag aattcattaa
     9961 taactccggt ggctgattca accgccaaac tcgacctcca ggaccattgg gatcgaaaag
    10021 aaaaagttca agagaatgtc tgtcctgatt attatggaga atcctttctg ctacacttaa
    10081 tcctctaggt ccggcaccaa tgatccctat tttcatttgc atgcctccat ttcgataaat
    10141 tttttcaata aaacagcatg gttctgatgt tcattttttg caccataaac aaaagtaatg
    10201 actggatgtt ctttcataag ttgactgagt ttaaaaatag cttgcttctt ttcttcttcg
    10261 aaaatcaatt cttgctggta tttctttgca aatgaagcaa atcgttctgg tacatggtca
    10321 aaagttttgc gtgcttcatt actaggtgca atttctttca accacagatc taactgcgct
    10381 ttttcttttg aaattcctcg tggccagaat ctatccacga gtattctata gccatcctct
    10441 ttttcagcat caaggtaaac acgttttaat ttgatcatag ttgtttcctt ctttctacct
    10501 tttcgcttag cttatatatg tagataatag cacaaaataa aaa
//
LOCUS       DASNNU010000089        10151 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.089, whole genome shotgun sequence.
ACCESSION   DASNNU010000089 DASNNU010000000
VERSION     DASNNU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.089"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            45..194
                     /locus_tag="U8116_002229"
     CDS             45..194
                     /locus_tag="U8116_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEN8435188.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            205..1719
                     /gene="dltA"
                     /locus_tag="U8116_002230"
     CDS             205..1719
                     /gene="dltA"
                     /locus_tag="U8116_002230"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEN8435189.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1716..2921
                     /gene="dltB"
                     /locus_tag="U8116_002231"
     CDS             1716..2921
                     /gene="dltB"
                     /locus_tag="U8116_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEN8435190.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            2966..3199
                     /gene="dltC"
                     /locus_tag="U8116_002232"
     CDS             2966..3199
                     /gene="dltC"
                     /locus_tag="U8116_002232"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEN8435191.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3202..4470
                     /gene="dltD"
                     /locus_tag="U8116_002233"
     CDS             3202..4470
                     /gene="dltD"
                     /locus_tag="U8116_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEN8435192.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4611..5693
                     /locus_tag="U8116_002234"
     CDS             4611..5693
                     /locus_tag="U8116_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEN8435193.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5693..6496
                     /locus_tag="U8116_002235"
     CDS             5693..6496
                     /locus_tag="U8116_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN8435194.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6566..7306
                     /locus_tag="U8116_002236"
     CDS             6566..7306
                     /locus_tag="U8116_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN8435195.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7317..8321
                     /locus_tag="U8116_002237"
     CDS             7317..8321
                     /locus_tag="U8116_002237"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEN8435196.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8366..10063)
                     /locus_tag="U8116_002238"
     CDS             complement(8366..10063)
                     /locus_tag="U8116_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435197.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3254 a         1686 c         2062 g         3149 t
ORIGIN      
        1 aaattagtta cactttgtgt gaggttatat gaggagggaa tccaatgaac aaatggcgtt
       61 ctaatccaaa attgaattat tggggaaaat tcatcattcg aacaattttt tatagtgcag
      121 tactgctgtt cctgatttat ctgtatcact ataaaaatat tcaaggagga acttttatct
      181 ataacgaatt ttaggaggaa gagtatggaa atcaaaacga ttattgaagc aatcgatgat
      241 tggggaataa atgaacctga tcgagttgct tatcaggcgg atgagacgca tacttttggt
      301 gaattgaaac aagcatcaga cgcacttgct tattatttag aaaaaaaggt agaaggcgat
      361 ggaccaattg tcgtttttgg caatttggag tttgaaatga ttgtctcatt tctaggggtt
      421 gtcaaatctg gccatgccta tttgccgatt gaagagcata cacccaagga gcgtatccta
      481 tcgatctttc gggtggctaa accatctatg gtcatcagta tcggggactg gatagaagat
      541 attccttcgg ttcctgtcat aacaaaaaaa gaatttcaag aaatcaagaa gataccagta
      601 ggattttatc ccagaaatag cgtaaaaggt acagaaaatt tttatattat ttttacttct
      661 ggtactactg gagaaccaaa aggcgtacaa atcagtcatg ataatctagt cagctttgtt
      721 caatggacga tggaagactt tgggatcaag cctggtatgc atttcttagc ccaagcgcca
      781 ttttcttttg atttatcggt attttctatt tatcctgcat tagtttctgg cggaatgctg
      841 aagccgttaa ataaagcagt tgttcaagac tttcgtcaat tattcgcgac attgccagcc
      901 ttgaagttga atgtgtgggt ttctactcca tcatttatgg atatttgctt gatggaacct
      961 acattcaatg gagaaaatgt gccggaattg gacatgtttt tattttgcgg agaagaattg
     1021 acgaaaaaaa ctgcagaaag tctacttgat cgattcccaa atgcacggat atataatact
     1081 tacggtccaa cagaagcaac tgtagctatt tcaagtattc agattgacca gcatgtacta
     1141 gatagttatg accgactacc gattggctac gtaaaaaaag acacgaaagt ttctattgtc
     1201 caagaaggta aacctgtttc aaaaggagaa acgggtgaaa tcattattgc aggaccaagc
     1261 gtatcaaaag gttacctgaa caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat
     1321 gacttagcaa gttaccatac aggggatgca ggtagactag atgaatatgg gcttttattt
     1381 tatgaaggac gtatggatca gcaggtcaaa ttacatggat accggattga actcggggat
     1441 atcgaacatt atctgctcca agacaaccga atcaaacaag ctgtcgtcgt accaaaatac
     1501 caaggaacaa aagtacagca gctggttgct tttgtcgtat tagaaaacaa aacgaaacag
     1561 ccggattttc agttgactaa atctatcaaa gaacaattac tagaagttgt aatggattat
     1621 atgattcctc agaagttcca ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc
     1681 gatcgaaaaa aattaacagc tgaggtgaat cctgaatgat gttacctcat atgatccctt
     1741 atgcagagcc gttttatttt tttcttttag ccattgcgct tgtaccgatc gttctatctt
     1801 tattgatatg ggaaaaaaga ttaccagttt atcaatcagc agtgacactt ttctttttat
     1861 ttgtcagctt tggaggtaat tattggcaac agggagcggc gctgattggg tatgttttgt
     1921 ggcagacgat acttgtatgg ggctattttg tttatcggca aaggaaaaat cagacaggat
     1981 ggttttttgc cgccgttatt ttagcgataa ttccactgtt tttaacgaaa gtgactcctt
     2041 tttttgaaca tggaaaagca tcgttacttg gttttttagg aatctcttat ttaaccttta
     2101 aatctgtaca aatgatcatg gaaataagag atggcatgat caaaacatat aagccatttc
     2161 gttatatcca gtttctgctg ttctttccaa caatctcttc tggaccaatc gatcgctata
     2221 ggagatttga aaaagatcag ctccatccgc ctgttcgtga taaatatgtt gatcttttgg
     2281 gaaaaggcgt ccaaaatatt tttgtcggtt ttttatataa attcattatc ggttattatt
     2341 ttggtcaagt actgcttcct gtagtgggac gcattgcctt acagcatggt ggtatttcat
     2401 gggcattagt aggttatatg tacgtgtaca gtatgtattt gttttttgat tttgctggct
     2461 atagcttatt tgcagtcggt acaagctaca tgatgggata tcagacacca atcaatttca
     2521 ataaaccttt tttatcatgg aatatcaaag aattctggaa tcgttggcat atgacgctgt
     2581 ccttttggtt ccgtgattat gtatatatgc gattgatgtt cttcatgatg aagaaaaaat
     2641 tttttaaaag caagatcgta atgtcaaata tcggttattt tgctttgttt cttctaatgg
     2701 gtatatggca tggattgacg tggtactaca tcgtatatgg actgtatcat gcttgtctga
     2761 tctgtctgac agatgcatgg atccgattta agaagaagca taaagagcag ttgccttcta
     2821 acaaatttac gcatggctta gccgtatttt tgacatttca agcagtatgt tttagcttcc
     2881 tgattttttc aggattttta gataaacttt tttttagcta acgaatagtg acatattaat
     2941 ttatataaca aaggagaaaa ggaaaatgga agaacaaatt ttatcaatct tagaagaaat
     3001 aacaggaaca gatgaagtaa gaaaaaatcg tgatattgat ctatttgatg aagggttgct
     3061 tgattcttta gcatctgttc aattattagt cgagctagac ggagagctgg atatccaagt
     3121 ccctgtatct gaattcgagc gagaggattg gtgcacaccg aataaaatca ttcaacaagc
     3181 aactgcattg aagggttgag tatgaaaaag aaactttttg ctattttcgg cccggttgtt
     3241 ttagctgttg gcttactgtt tgtgtttttt acctcctcct atcaagtaga tatctctaat
     3301 ccagaaacga ttagacgtgc ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg
     3361 ataaaaaatt atgcattatc tgaaaaacaa tatattcctt tttttggctc aagtgaatta
     3421 agcagaatca gtccatttca tccgtctgtt ctggcagaaa aataccagcg aaactatcgt
     3481 ccatttttgc tgggagcacc cgggacacaa tcgttaagcc aatatatgat gatgcgttct
     3541 gcaggagatg ctatgaaaaa caagaaagtc gtctttatta tttctcctca gtggttcgtt
     3601 aaaaatggtg taaaaactga ttactttaat acgtattatt ctgaacttca aacatacgac
     3661 tggttgtttt caatgaagaa agtaacgcct gctgatcgct atttagctag aagattatta
     3721 actttctcaa aagtaaaaga gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa
     3781 gggaagctac ctttacctga atctttgaat cagctgcgca gccaatggaa catgctgaag
     3841 agagaggatg aagtattcag caatattggg ttgaaagatc gacagcaaaa aatcgatcat
     3901 gagtcaaaac gattaccaaa acaataccaa gaaacagaat tatccatttt agcaaatcaa
     3961 attggagaac gagaaacgac gaataatcca ttcgggttga aaaacgattt ttatacgcat
     4021 cgtatccgag cccacgagcc agagctcaaa cagtcacaaa aaaattggga ttatcgtttt
     4081 tcgccagaat tttctgattt tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa
     4141 gtattgttca tcatcccacc ggttaacgag aaatggagcg attataccgg actatctcaa
     4201 gaaatgttac aaggatttgc caaaaagatc aaatttcaat taaatagcca aggattcaat
     4261 cgaatagctg attttgtcaa tcaagcgggt accaactatt ttatggaaga cacgatccat
     4321 ctaggctgga aaggctggtt ggctgcagat caacagatca gaccgttttt agaagaaaat
     4381 catattactg ctagtaagta tcatcttgac gatgcttttt ttagcaaatc ttggcaacat
     4441 caaataccag ataaactaca attaaaataa agaaaacagg cgagtgttgc tcgcttgttt
     4501 tctttatttt tgaatattct gaataaaaga taaaaatacc aagtttgcct gaacaaaaat
     4561 cataagcaat ggtataatca caatatgaat atagaaatgg gggaaaacat ttggatacaa
     4621 aagaagaaca gctacttatt gatttaactt ctgcaaaagg agtacctgga aatgaagaag
     4681 aggtaagaga agtattcaga gaatatgcca aaccattcgc tgatgatatt ttttatgacg
     4741 gtttaggcag tgtgattgcc aaacatggtg ctggcggagg ccccaaggtc tttatttctg
     4801 gacatatgga cgaagttggt tttatggtaa caaaaataac cgaaaaagga ttcttggaat
     4861 tccaaactct tggaggttgg tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa
     4921 cacgtgaagg gaaaatcgtc cacggtgtga ttggttccaa accaccacat gttttaacac
     4981 ctcaagtaag aaataaacct tatgaaataa aggatatgtt cattgatatt ggcgcatcaa
     5041 gccaagaaga agccaaagag tggggcatac gtcctggaga tatggtcact ccatatattg
     5101 aatacaaacg aatgaatggc agtaaatatt tactggcaaa agcatgggat aatcgtatcg
     5161 ggacagctgt ttctctgcga gtattagaga atctatctaa agaagcacac ccaaatgttt
     5221 tattcgcagg aagcgatgtg caagaagaag taggcttgcg aggtgctcga acaagtacac
     5281 accttgtaaa tcccgatatt gcttttgctt tagatactgg tacagcagga gacactccag
     5341 gaatgacacc aaaagaagca gattcgattt taggaaaagg cccacagatt ttgatttttg
     5401 atgcatctat gatcccgcat aaaaaattat tgaattttgt tattgatgta gcagaggaaa
     5461 tggatattcc attccaatat acagtgattt ctggcggagg aactgatgca ggacaaatgc
     5521 atttgacaag agatggtgtt ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt
     5581 cccatacatc agtgattcat gaagatgatt atttgaatac agtgaaacta gtcacagaat
     5641 tagtgaaacg gcttgatagt gaaacagttg aaaaaattcg gagttatgaa taatgaataa
     5701 tagaatacct atttctgaat atcggaaaaa agcacgagtt tctttggaaa atcaatgggg
     5761 aatcaatact tggcttgttt tcttatctat aatatttacg agcgtcatcc agaacttttt
     5821 tgacggtttc agtccttttc cggaagaaag catgcagcag cacttattga gttttctagt
     5881 cgataattta cttttattcg cattcaacta tgcattgttt tacattgcgc tagtagttgt
     5941 acggggtggc agaccaaaaa gtaacctttt attttcagtt ttccaaagaa agtattttgg
     6001 ttcgatactt ggcatcaatt tgatcaattc agtcatcaac tggctactca acagtttaat
     6061 ctttctgccc atgtttttaa tcagcggagt gagtacttat atgcagctgc ttttcagttc
     6121 aaatgcggta cctacgggtg tcatgccaga agattttctt gatatcagct ttgttttatc
     6181 gatgataggg atgatttttg tttcagcact catcatgagt attgttggcg gtttattcca
     6241 atttgctgca tggacaaagt tcgactatcc tgatttgtcg gttattcaat gtttgaaata
     6301 tggctggttt ttattgaaag atagaattgg tacgtatatt ttgttgcagc tgtcttttat
     6361 cggctggtat ttgctaggag caatcgcttt gttcatcggt cttttgtggg tcattgttta
     6421 tgtgaatgtg tcagttgcag aattttatga acaagcacgg attgaaaaag gaggtccaga
     6481 cgcctatttt acttagcatg taagcagttt attcatcaaa aaaataactt ttgtggtagg
     6541 ataaatcttt gagggggaat gtacgatgaa attaactgtt ttaggatgct taggagcgta
     6601 tccttataaa aatcaaggga caacaggtta tttgcttcaa tctgacgggt tcaatttgtt
     6661 gatcgatgca ggtagtgcta cgctgatcaa attacaagaa caccttgatc cacttgatct
     6721 agatgctgtg atccttagcc attatcatca tgatcatatt gctgaccttg gtgtattaca
     6781 gtattactgg caacttcatc ctaagagaaa tccggagaca ttgctttcaa tttatggaca
     6841 cacaaaagat tccattcact ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc
     6901 ttattttgaa gctgaagaat taaaaattgg tccttttctg attactttta tggaaacaat
     6961 ccatccagta gtatgttacg ccatgcgtat cgtagaagaa acaacgggga aagtattcgt
     7021 atttacaggt gattcaggtt atttggaaag tttcgctgat tttgcgaaag atgctgattt
     7081 gtttctagca gatacctatc tttttgaagg aaatgaacat catcatgccc atttcacttc
     7141 aaaagaagct ggagaacttg cgaaatcagc taacgtaaag aaacttgtac ttacacacct
     7201 acctcagttt ggtgatttag aacaattgaa aaatgaagcg caaaaggcag caggcaaagg
     7261 gattgatata gaacttgctg cagttgacaa agtgtttaac atttaggagg aagaacatga
     7321 tttttgtacc aaatgaaaac cacgacccaa gaatcaattt agcaattgaa caatatttgc
     7381 tgcaagaaat gcctttagat gaacctattt tgctctttta tatcaatgaa ccttcaatca
     7441 tcatcggcag aaaccaaaat acgcttgaag aaatcaatcg agattatgtg gaagaaaatg
     7501 gcattcatgt tgtccgtcgc ttaagtggag gcggagctgt ctaccatgat tttggaaacc
     7561 tgaactttag ttttatcatg cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa
     7621 cacagccaat catccaagca cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc
     7681 gaaatgatct agtcattgat ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg
     7741 gtcggatgtt tgctcatgga acaattatgt tcgactctga tatcaatgaa gtagtcaatg
     7801 cattaaaagt aaaaaaagac aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg
     7861 taacgaatat caaaccattt cttccagaag aaaagcaaaa aatgacaaca gaagaatttc
     7921 gtcaagaaat cttgttgaaa attttcggcg tagagtcagt agaagaagta aaaacgtacg
     7981 aattgactga tgaagattgg aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt
     8041 gggattggaa ttacgggaag tctccagatt tcaatttctc tcgccaaaaa cgtttttcta
     8101 tcggttcgat tgaagtccat ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct
     8161 ttggcgattt cttcggacta ggagatatct ctgacgtgga aaaaaaattg gttggacaga
     8221 aatatgataa agcatctcta tcaaaaatag tcagccaaat tgatgtgaag aaatattttg
     8281 gtgcgataga accagaagaa ttgcttacct tactttattg aaaataaaaa atagcggaat
     8341 ccacatatat ggattccgct attttttata caggattcgg gtaatttcga gagagttgaa
     8401 cagtgatccg atcaatgaaa gtatcttttt cctgcgttag tgttcctgtg taacgatccg
     8461 ctacgtcttt catcatcatt aagtcaaaac gttcctgatc atctaattct tctgtgatgc
     8521 ctcgactaaa taggtttgta tcgtaatgtg cactatgttc gcaacaaatg acgaaatgtt
     8581 cctcttgttc gtgtatcgac aaataaattt ttcgatacag tggattttcg atggcatgag
     8641 tttctctaaa ggaatgctct aaagcgtgga tcaaaagttg agccagatcg tcatcgttga
     8701 taggaagttc ttcgggaaga tctgcttgga aggttatctg aatattccgt ttggcagcta
     8761 acttctggta acgcgctaag ataagatttg tcacctggtg gttgcaaaaa gaacacagca
     8821 tttcgaaatt ttttgcttct tctaatatct tttccacata gtttttagct tttgtttggt
     8881 ccgtatgcat taaatcttgc agattctcca tctcttggac aaattccagg cgaagtgtat
     8941 ttacttcttg gatatgctgt cgaagctgct cataatgttg ctccaacaaa tcatttttga
     9001 tttttaagaa atctacttgt ctccgatctt tgtcggtatt taagatgtga tcgattagat
     9061 tgtagctgat aataacgatt agaatgataa tatataaaat cgtagaccaa ttgatcgttg
     9121 tttgatagat acctaatgcc gtttggatgt aaatcaagca atggataatg gctaccaaga
     9181 cgatccaagg tgtacaagct acataaaaac ggttattctt atatgcttcg ccaatgcatg
     9241 caagtgaagt gactaaagtg cttataacac tagcaatatt agcaatcggc attaattcag
     9301 gaagttctgc aggagtaaaa gttgcataaa gcagaacgcc gataaaaatc aaaagttgga
     9361 acgtacaaaa aattccgtac cattttttat agtaaaacat tcggctccaa tagtagcaga
     9421 tgatgaaagc tgacgttaag ataataaata aatgagataa aaaagaatga acgaatggtt
     9481 caaaaagaat accacttaaa atatcttctg atacagcttc aaatcctaat gccaaagcaa
     9541 agcaacttaa gagaataagt gagtattcaa gacgtttttg tttatacatc aagtagatcg
     9601 tatagaaaaa ggtccctata gcgataaaaa cagcgattat catcgtcaat acttgcggca
     9661 ctgattgtga ataaataaaa ctaatcaatg gtccagttgt tcctagataa actttcggtg
     9721 gcagtccagc atagttctga taaggagatg agactgtaag ggtcaatttt tttccgatat
     9781 aatttgctgg taaatctatc acagccaatg agagtccggg attgctttct tcctgataat
     9841 ctttccgttg gtataaaatc ttttcgccta gatatacagt aatccattga tggtttcctt
     9901 tgattagtaa atttgcttcg tccaacggtt tttcaagtgt cgtttccata atgaatgttt
     9961 cgtctttatc cacggtagga atcgtacgag tgtacttgct gtcaaactta aaatcaggat
    10021 catcttttgt atagaaaacc cacgaatctt ttatttcttt catttgattg ctgtccgcag
    10081 gagaaaaaat aaaataaaag aaataagcaa ttcctgttaa aaataacaat gataatataa
    10141 tgattatgct c
//
LOCUS       DASNNU010000090        10020 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.090, whole genome shotgun sequence.
ACCESSION   DASNNU010000090 DASNNU010000000
VERSION     DASNNU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.090"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..365)
                     /locus_tag="U8116_002239"
     CDS             complement(45..365)
                     /locus_tag="U8116_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN8435198.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(513..2747)
                     /locus_tag="U8116_002240"
     CDS             complement(513..2747)
                     /locus_tag="U8116_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN8435199.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3226..3492
                     /locus_tag="U8116_002241"
     CDS             3226..3492
                     /locus_tag="U8116_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEN8435200.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3492..5219
                     /gene="ptsP"
                     /locus_tag="U8116_002242"
     CDS             3492..5219
                     /gene="ptsP"
                     /locus_tag="U8116_002242"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEN8435201.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5383..6429
                     /locus_tag="U8116_002243"
     CDS             5383..6429
                     /locus_tag="U8116_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN8435202.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6446..7669
                     /locus_tag="U8116_002244"
     CDS             6446..7669
                     /locus_tag="U8116_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN8435203.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7758..8645
                     /locus_tag="U8116_002245"
     CDS             7758..8645
                     /locus_tag="U8116_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN8435204.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8731..8961
                     /locus_tag="U8116_002246"
     CDS             8731..8961
                     /locus_tag="U8116_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEN8435205.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9018..9983)
                     /locus_tag="U8116_002247"
     CDS             complement(9018..9983)
                     /locus_tag="U8116_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN8435206.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3185 a         1777 c         2027 g         3031 t
ORIGIN      
        1 ttacttttgt cccacgctct ttaattatta tgttcaaaat ttgtttacac ggctaatgta
       61 gcgcctaatt catgttttgg aatcgagatt tcaacaaggc ctgccaattc gattactttg
      121 atttcatcct cgtggtaaac ggcgtctttg attttatcta agaaataatt tttagcatct
      181 tcgatttctt tcggctggat tttacgtttg tcattgatca gcagaatttc attatgttga
      241 ggggcttcag tgtagacaat agtggtattt ccggctgtgt agaccttgac tagatgggca
      301 tcagtgtttt caagctggtt taatacaaga cgggaatggc tgtttgttac atttaccaat
      361 ttcatggcgc tcacctcttt tatgtatttt taattgcttg tttctcttat tataatttct
      421 tttttataaa atgaacagta ttttgacttt tctgaaaaaa gaatgagacg gcatttctca
      481 aagaaatacc gtctcacgct ataaaactgg atttattcag ccgtttcttc ggctgattct
      541 ttagccaaac gttctggttt agaagtaacg atgatttttc catctttatc cagttttgct
      601 ttcaactcat ggatagaagg gtgatcaaga taaaattcag cgataccatc ttcgatttgt
      661 tcttggatcg tgcgtcgaag aggacgagca cccatagatg gatcatagcc taagtcaact
      721 aatttttctt tgacattcgt aggaacttcg atgtgaagtt gctgtgctgc cagcatttgg
      781 ttgacatcgt ctagcattag tgtgacgatt gtcatcaagt tttcttttga taatgcactg
      841 aattcgatga tcccgtcaaa acggttcaga aattctggtg taaagtagtt gtttaattgg
      901 ttcaatacag aacgagtaac accttcgcgt gccgcaccaa aaccaacgtt tgcttctact
      961 ttaccagttc cggcattact tgtcataatg atgatcgtat ctttaaagct gaccgttctt
     1021 ccttgtgcat ctgtcaatcg accatcatct aagatttgca agaacatatg caaaacatct
     1081 ggatgagctt tttctacttc atctaacaag acaagactgt aaggatttct ccgtactttt
     1141 tctgttaatt gaccagcttc ttcgtatccg acataacctg gaggagaacc aatcaattta
     1201 gagacactat gtttttccat atattcagac atatcgaaac gaatcattga atcttgtgaa
     1261 ccgaatagtt cgtaggcaag ttgtttcgca agctctgttt tacctacacc agttggtcca
     1321 acaaataaga aggagccgat tggacgattc tgtttgttta atccaacacg gtttcggcga
     1381 atcgctttcg ctactcgatc tacagcgtta tcttgtccga tgacatgagc tttcaagtca
     1441 tctgctaagt ttttcagttg tgtctgttct ttttctttta attcacccac tggaatacct
     1501 gtccgttgtt caacgatttt ttccatatct ttttcggtaa tcacaggtgt ttcctcttca
     1561 gtgagctgac gttctttcat tttttgaagt ttattgatct gatcgcgata ataagctgct
     1621 ttttcgaaat cttcttctcg agaagctaaa actttttgtt cttctgcttc ttgcagtttt
     1681 ttctcgattg ttttaggatc aacgatttgg atcgtcaagt ttttcttaga acctgtttca
     1741 tctagcaaat cgatggcttt atccggcaag aagcgatctt ggacgtaacg attggataaa
     1801 gttgcggctg cttcgatggc ctcatcagta tattttacat gatgatagtc ttcataacgt
     1861 ttttgcaaac ctttcaaaat actgatcgtt tcttcgacag ttggttcatc tacccgtaca
     1921 ggttgcatac gacgttctaa agcagcgtct ttttcgatga tgcggtactc atttaaggta
     1981 gtagcaccaa ccatttgcaa ttctcctcga gctaaagctg gttttaaaat attaccagca
     2041 tccatatttc catcaccagc agaaccagca ccgactattt cgtgaacttc atctatgaat
     2101 aggatcacat tttctgcttg acggatctct tcaatcaatt tttgcatacg ttcttcaaat
     2161 tgtcctcgta taccagtacc ttggactaaa gatacgacat ctaaacgaat gacttctttg
     2221 tccattaatt tttgcggtac atcaccatct acgatttttt gtgctaaacc ttcaacaact
     2281 gctgttttac caacacctgg ttctccaatc aagacaggat tgttcttggt tcggcgattt
     2341 aaaatttcaa tgactcgttt gatttcgtca tcgcgtccga tcacaggatc gatatcacct
     2401 tgtcgtgctt gttctgtgat attgattccg tattctccca ataaaccttc tgtattgcct
     2461 tgaggtggct gaccaccatt gaagttgttg tttccgccaa attgtgttgg tggtgtttgc
     2521 tcataatttc cttgctgttg catttggcga gacatggaac ggaaaagatc gtccaaactg
     2581 ccaaatccaa atggatcatt ttgggccata ttagtcagac ctccattttg ttgatttttg
     2641 attttttgat aacagctctg acagtaatcg agttgtgtcc gctgaccatt gacagtggca
     2701 tacaagtgaa tcgttgcctc attcttgccg cagttttgac aaagcatgaa tcattcacgt
     2761 cctttcacaa aaatgggtac atccatattt tacatctcgt ttttcatgag gtaaaagaat
     2821 aacccctagt tttttaagag atttatgagg tagaaaaggt gaaatctcta ttgaccattc
     2881 ctgacctttg actcttatta tactaaaaat aaaaagggaa gcaagtaatt cgtttcatat
     2941 gaaaacaaag agggtcaaag gaaagaatga taaacgtcag gagaagagaa agcgacaaga
     3001 aaaaggaaat aaacacgttt gttataaaat gtgaagaaaa ggatgaaaaa gaatccggaa
     3061 taatttgaat acttttatct gaaattgaat gaaaactgtt gtatctaacg aaaaaaaatg
     3121 ttaatcttta cagatgaaag ggtggcattt gttaggattc tctgaattac taaaaaagaa
     3181 aaccctcaca taaaaacaac actcacttaa aggagaccga ttaatatgga aaagaaagaa
     3241 tttcacgtag tagcagaaac tgggatccac gctcgtccag ctacactatt agtacaaaca
     3301 gcaagcaaat ttaactctga tgtaaactta gagtataaag gtaaatcagt aaacttgaaa
     3361 tcaatcatgg gcgttatgtc tttaggtgta ggtcaaggtt ctgatgttac tattactgca
     3421 gaaggtgctg acgaagctga tgcaatggca gcaatcgttg aaacaatgaa gaaagaaggc
     3481 ttatctgaat aatggttgaa atgctaaaag ggattgccgc tagtgacggt gtagccgttg
     3541 caaaagctta cctgctagtt caaccggatt taacattcag taaggcaaca gtagaagata
     3601 ctgcggctga agaagctcgt ttggatgctg cattagctaa atctactgaa gaattgcagc
     3661 aaattcgtga aaaagcagcg caaagcttag gtgaagcaga agcgcaagta tttgatgccc
     3721 atttgatggt tctttcagat ccagaaatgg taggtcaaat caagcaaaat attaaagaca
     3781 acagcgtaaa tgctgaatca gcgctaaaag aagtaacgga tatgtatatc ggtatgttcg
     3841 aagcaatgga agataatgct tatatgcaag agcgtgctgc agatattcgt gacgttgcta
     3901 aacgtatttt agcgcactta ttaggcgtta ctttaccaaa tccatcaatg atcaacgaag
     3961 aagtggtcgt tgttgcacat gacttgactc caagtgatac agcacaatta gatcgcaatt
     4021 ttgtaaaagc gtttgtaaca gacattggtg gacgtacttc acattctgct atcatggcac
     4081 gttctttaga aattccagca atcgttggga caaaagaaat tactgctaaa gtcaaagaag
     4141 gcgatatttt ggctgtcaac gggatcgaag gcgatgtaat catcgatcct acagatgaac
     4201 aaaaagctga atttgaaaaa gcaggtgcag attacgcagc acaaaaagca gaatgggaaa
     4261 aattaaaaaa tgcagaaact gtcacagctg acggcaagca ttttgaatta gctgcaaaca
     4321 tcggaacacc aaaagatcta gttggtgtcc ataataatgg tggagaagca gttggtctgt
     4381 accgtactga attcctgtat atggattctc cagacttccc aacagaagat gaccaatatg
     4441 aagcttacaa agctgtatta gaaggtatgg aaggaaaacc tgtagttgtt cgtacaatgg
     4501 acatcggtgg agataaagaa ctaccatatc tgcaattacc tcatgaaatg aatccattct
     4561 taggatatcg tgcattgcgt atcagtttat ctgaacaagg cgacgaaatg ttccgtacac
     4621 aaatgcgtgc attgcttcgt gcatctgttc atggtaattt acgtatcatg ttccctatgg
     4681 tagctacact caaagaattc cgcgcagcaa aagcgatctt tgaagaagaa aaacaaaaat
     4741 tgataagtga aggcaaggaa gtatctgata cgatccaagt cggcatcatg atcgagattc
     4801 cagcagcagc agtcttagca gacaagtttg caaaagaagt agatttcttc tctgtaggta
     4861 cgaacgattt gattcaatac acaatggcag cagaccgtat gaacgaacgc gtttcttact
     4921 tgtaccaacc atataaccca tcaattttac gtttgatcaa aaatgtaatt gatgcagcac
     4981 acgctgaagg aaaatgggca ggtatgtgtg gagaaatggc tggagatcaa acagctgttc
     5041 cattgctagt aggtatgggt ctagatgagt tctcaatgag tgcgacatct attcttaaaa
     5101 cgcgtagttt aatgaaacgc ttggacacag cgaaaatggc tgaacttgct gatcgtgcat
     5161 tgaaagaatg cgatacgatg gaagaagtcg ttgaacttgt tcatgaatat gtaaaataaa
     5221 caacgtatca aaagctggtg tggcttgtaa aagagctacg ccagtttttt tgttcttttt
     5281 taagaaaacg tttttgtcag taagaataga caaaaatagg gcttaagttt gagtgacagt
     5341 ttgtaggcta tgttatacta tataagtgaa gggggacgta aggtgaaggt attattatat
     5401 tttgaaagtg aaaagatgct tgcaaaatct ggaatcgggc gtgcgcttga tcatcaaaaa
     5461 cgtgctctga cggaagtcgg tatctcttat acactagatg aaaaagaaga ttatgacatc
     5521 ctgcacatca atacatacgg aatcaatagt cataatatgg tcaataaagc cagaagagaa
     5581 ggaaaaaaag ttgtctatca tgcgcattct acagaggaag atttccgaaa ttcctttatc
     5641 ggatcgaacc agctttctcc tatcgtaaaa aaatatctgg taggattgta ccaaaaagcg
     5701 gactatctga tcacgccaac accttattca aaacagttgt tggaaagcta tggtatccgt
     5761 gtgccgattc aagctatttc caatgggatc gacttagaga aatatcaccc tgatccaatg
     5821 aaagaacaaa agttcagaga atatttcaaa ctgtctccgg atcaaaaagt aatcatttgt
     5881 gtcggtttgt ttttcgaacg taaaggaatc attgatttcg tagaaattgc taagaagatg
     5941 cccgaataca catttatttg gtttggtcat gttcccatgt actcgattcc tcggaacatc
     6001 cggaaaattg taaaagaaga tcatcctgaa aatgtcctgt ttcctggtta cattcgtgga
     6061 gagatcatag aaggtgcgta ttcaggtgca gatctattct ttttcccttc ttatgaagag
     6121 acagaaggga tcgttgtttt agaagcttta gcaagcaaac aaaacgtact tgttcgagat
     6181 attccagtgt ataatggctg gttagaagat agcaagaact gctatatggg gaagacaaac
     6241 gaagaattta tccgactgat ccaacagatt accaatcaag aactaccgag tacgacacaa
     6301 gccggttatc aaacagcaaa agagcgaagt atcaataaaa tcggtgaaga acttaaaaat
     6361 gtatacgaaa gtgtactgaa tgaaaaagtc tctagcaaat taaaaaaagt aaatcaggtc
     6421 aaggactagt ttggaggcga aaaacatgaa aatcggcttt tttaccgata cctactttcc
     6481 gcaagtcagc ggagttgcta catcgatcaa aacattaaaa caagaattag aaagacatgg
     6541 gcatgaagtc tatattttta caacgaccga tcctaatgcg gataagttgg aaaaagatgt
     6601 gattcgaatg ccaagtgtac catttatttc tttcaaggat cgaagagttg ttgttagagg
     6661 catgtggtat gcttatttgg ttgccaaaga actagaacta gatcttattc atacccatac
     6721 agaattcgga gcagggatac ttgggaagat ggtagctaag aagttgaaaa tacctctgat
     6781 ccatacttac catacgatgt atgaagacta ccttcattat atcggtaaag gaaaagtgat
     6841 caggcaatcc catgtaaaat acttgtctag attatttgca aatcatacga caggtgtagt
     6901 ttgtccaagt gaacgtgtta ttgatacatt acgcagctat ggtgtaatag caccgcttcg
     6961 cgtgattcct actggtatcg acattgaaaa attcaaacgt tctgatatca ctcagcagga
     7021 tatcagcaaa ttaagggagt cactaggtct tcaggaaaac catttgatga ttctttcttt
     7081 aagcaggatt tcttatgaga aaaatatcca agccttgatt aatgggtttc ctcaggtcat
     7141 ctccgcttat cctaacgcca gattagttat cgtaggaaaa ggaccttatg tagaagagtt
     7201 agaggaattg atctcagaac tgcagttaga agaatttgtg cagttcacag gagaagtgga
     7261 caacaatgat gtagcacttt attataaagc ggcagattat tttgtcagtg cttctacttc
     7321 agaaacacaa gggcttactt atacagaagc gatggcagcc ggaacacctt gtgtcgtgga
     7381 aggaaatgct tatttgaacc gattgttcga tcatccttca ttagggaaaa cttttgaaac
     7441 cgacgaagac tttgcaccag cacttattga ttatatttcc agtgaagttc ctaaagatcc
     7501 aaaagtttta gaagctaaac tttatgatat ttcatctaca catttcggcg aagctatgat
     7561 cgaattttat caagacatgg ttgtttatca tgaagaagag atgcgaagaa aagaagaaga
     7621 agactcgatt gaacgaatca aaataaaatt aaattcattt aaaagataaa attcaaacat
     7681 tttttatgaa aaaatccttg tttctagcgt atactagcat agaagcaagg atttttgatt
     7741 tttaggaggt cgaaaaaatg aaacttggtt ttatcggaac aggcgtcatg ggaagtgctg
     7801 tcgcacgcca tctactagaa gcaggacacg aagtagctgt ctataatcgg acaaaagcaa
     7861 aagctgatcc tttagtaaca gaaggagcta tctgggcaga tacaccaaaa gcggtagcag
     7921 aacaaagtaa tattctcttt acaatggtag gttaccccaa agatgtagaa gaaatctact
     7981 atggacagtc ggggattttt tctgcagata tctcaggaca tattttagtt gatttgacta
     8041 caagtacacc aagcctggca gaaaaaattg caaaaacagc aaaagaaaaa ggcgcagatg
     8101 cgttagatgc accggtatcc ggtggtgact taggagcaaa gaatggtaca ctgacgatca
     8161 tggtcggtgg agaagaagcc gtttatgatc aagtactgcc tttattcaaa gaatttggta
     8221 cgacatttac attgcatggt tctgctggga aaggtcagca tacaaaaatg gctaatcaga
     8281 tcatgattgc aggtacgatg acaggaatga cggaaatgct tgtctatgca caaaaaaacg
     8341 gacttgattt aaagaaagtg attgagacgc tttcaggtgg aagtgcagct aactggtctt
     8401 taagcaatta ttctcctcgt attttaaaag aagattacac acctggtttt tttgtcaaac
     8461 attttatcaa agatttaaag attgcattag aagaagcaga aaaaatggat cttgtattgc
     8521 cagcaactac ccaagcattg aaactatatg aagagttggc agataaaggt tttgagaatg
     8581 acggaaccca agctttgatc aaactttggt ggcctgaagg aaaaatacca gaaaaaaaat
     8641 cataaaaaac gagttttttt agagaaaatc aatgcaagta ggctatgctt ttgatacaat
     8701 ataacagaaa taagaaaagg aggacctccg atgacacatt cacaattagt cgcaatcata
     8761 aaacgactgg aggctatggt cgagtcagca gataatgaac tgcaagttcg ccgttttgaa
     8821 aaagaaggcg tagagaagtg tacagtaact tatgataaag cgaccgatac atttgaatta
     8881 actgaaacag atactcatca gcaatacgaa tttgataata tcgatatcgt agcgatggaa
     8941 atctatgact taatccaata aattttagga taattgacat ttgaagttga agatgcctaa
     9001 aagaagccga gaagcaacta atttagttgc ttttcagctt cttttttgtt taacagttca
     9061 atttcaaact gttctttttc taattttttg cggtaactga tcaagatttg tacaggatcg
     9121 atttcttctt ttgacaattg acgtttcaaa gataaatgag cttctttctt ttcataatag
     9181 aagtcaggag aaacgtcacc agaggtaatc attgtttctg caagttgacc ataacaaacg
     9241 aggtagtgct ttttctgact ctcatctgct gctaatggca tgctgaaggt gagcgatggt
     9301 gttcttaaaa actgcttttg ttcctggatc cgtctaataa aataagtaac taattcgata
     9361 tctttttgaa gcataccggc tttttcgaaa ttcaactgat agatgaaatg ttgttgtctt
     9421 tttttcaagt aagtcagtag ctttttattt cggccttgaa acagcccttg gagttccttt
     9481 tttttctgtt caaaagtcat ttcctgtagg tcaggaagct ggactcgcaa agctaacaaa
     9541 ctaatttcag acaaccaagg catttgataa gtttcttcca ttatacgcaa aatagacggc
     9601 atttttttat agagacgaaa aggcccgtat gttcgagccg taggagtatc tgttaataca
     9661 aatcccttat tggtaatatg tacataatta taattactga aataattcat ttgtcggtta
     9721 taaaaaggac ggtaatgttg gatcaattga cactcgagca ataaagcatc taattccgtg
     9781 tctactacga cgaaatcaaa atctgttata tgatgaatca tacgtaatac tttttttgaa
     9841 tgctgtttat tctgatgaaa atagctgctt actctatttt tgagccgttt tgctttacca
     9901 acatagatga ccttaccatc tcggtctttc ataagataga cacccggcag catggggaga
     9961 gttttcattt tttcttttaa cataggatct ccttttacgc tttgaataaa aagactataa
//
LOCUS       DASNNU010000091         9934 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.091, whole genome shotgun sequence.
ACCESSION   DASNNU010000091 DASNNU010000000
VERSION     DASNNU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.091"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            456..875
                     /locus_tag="U8116_002248"
     CDS             456..875
                     /locus_tag="U8116_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN8435207.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            901..1107
                     /locus_tag="U8116_002249"
     CDS             901..1107
                     /locus_tag="U8116_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8435208.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(1335..1508)
                     /locus_tag="U8116_002250"
     CDS             complement(1335..1508)
                     /locus_tag="U8116_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435209.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            1484..2149
                     /locus_tag="U8116_002251"
     CDS             1484..2149
                     /locus_tag="U8116_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8435210.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(2231..3397)
                     /gene="dnaJ"
                     /locus_tag="U8116_002252"
     CDS             complement(2231..3397)
                     /gene="dnaJ"
                     /locus_tag="U8116_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEN8435211.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(3548..5377)
                     /gene="dnaK"
                     /locus_tag="U8116_002253"
     CDS             complement(3548..5377)
                     /gene="dnaK"
                     /locus_tag="U8116_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEN8435212.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(5428..5991)
                     /gene="grpE"
                     /locus_tag="U8116_002254"
     CDS             complement(5428..5991)
                     /gene="grpE"
                     /locus_tag="U8116_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEN8435213.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(6085..7128)
                     /gene="hrcA"
                     /locus_tag="U8116_002255"
     CDS             complement(6085..7128)
                     /gene="hrcA"
                     /locus_tag="U8116_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEN8435214.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(7362..8527)
                     /locus_tag="U8116_002256"
                     /pseudo
     CDS             complement(7362..8527)
                     /locus_tag="U8116_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            complement(8543..8770)
                     /locus_tag="U8116_002257"
     CDS             complement(8543..8770)
                     /locus_tag="U8116_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEN8435215.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            9254..9844
                     /locus_tag="U8116_002258"
     CDS             9254..9844
                     /locus_tag="U8116_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEN8435216.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
BASE COUNT         2816 a         2043 c         1736 g         3339 t
ORIGIN      
        1 gtatagaaaa acgtgttata tggtttatag gtttatattt aataaaaagc actactaaac
       61 gccaataaaa aaaaccgtta tatggtagtg ctatttacgc tgttaaaata ttgtatatta
      121 cttccaaatg gcggtttgtt ggaggtcaac gtcgccatga agtacatcat atacaataaa
      181 tttccttaca ttgggttctt gtcaaaaaaa gtcgtctatc tgcaatagat aagtacgtcc
      241 accaatgtgg ttttataaat catatagata gaataacaga agcatgtaaa cagagaaata
      301 aatctgttta tatgcttttt tggctattca gaactttttt acaaagttta tttatcagta
      361 atgcaacaaa tccccctttc acattgggac taagagtgaa aggagataaa cgagcaaggc
      421 tcacttcctt tcctagacag aaagggggtg agattatgag gacatcttct ttcgagcata
      481 tcgtcagatt acagttcaat tctttaatgc tgattgtcat taagaacaaa gtgaaaagtc
      541 gaaacagaca gcttgcaaga cattctaaac gtgaagtatt gttttgtgaa atagccgaaa
      601 cgaaacaagt tgaacgtgga acgattgata cctgttttta tgattgtgta tcatttaaag
      661 tcttgcactt tacaatttat gtatctgatg aaacacttgg tacagtttta catagactat
      721 cagaaaaaca gcgtagtgcg attttattac gttattttca aggaatgaac gacagagaaa
      781 tttcagaact gtaccatgta tcacgttctg ctatttgcag tagaagaact agaggactaa
      841 ggaagttaaa aatgcttttg agcgaaagga agtaaagatt atggctatcc ctcttttgaa
      901 ttgatttgca aagcgtcaag cggagatgaa atggcagtta aagagatttt gaagttttat
      961 gacggttata tttctaaatt atgcttgcgt cctttttacc actctgaaag tggtaaaatc
     1021 attatgcagg ttgatgaaga actaaaagga gaaattcata cagacatgat gaaagcaata
     1081 ttgaaatttg aaatcggagt gaagtaatca actgtataaa aaaatcggga gatacacagt
     1141 ttcgtgttat ctcccttagt agttttacgg tcatatcaag gctcattcaa tcgtggtaaa
     1201 ttcgtggtaa gatgagtgtt tttctatact tcaaaatgct tgaatgtata gtgtttatgc
     1261 ggataattga aaattagata taaaatttaa taaaaaccaa aaatgcacgg tcgccatgaa
     1321 gggaccgtgt atttttatct tcttattttc tcacctaatc gtatcagccg catcgcttgt
     1381 tttcctgcac aaacaagcaa tcgctctacc tgttccatcg cataagaaag taccatcggt
     1441 tcattgatga tatcaagtac atagtcgata ccaccctcac ttaattgagc ttgatttttc
     1501 ttctccatat tatgtagttg tgggacaact ttctgacaat cagcaattgt gtcaagaacg
     1561 gatcaatcct ttattggagt tgctcgaaag ggtcttctct tattacggac aggctttgct
     1621 cactgtacat agacaacaaa tcattatcct agtcaatcgg atttccagag cctcattgct
     1681 atcattgctt gataaaatcc agacaaagtt taataaaatg tatcagttac agctaaactt
     1741 tggaattggg tcgctttgtt atacagagca agagacaccg caatctttcc ttcatgccaa
     1801 acaagtttgc gaatggatag ctttccatca atctgtgaac gagatccgct tttttgaaga
     1861 tttagacctc gggatcgttt taccagcaat cccgtcagat caacgaacat tatatgtaaa
     1921 acgtattttg aaaagcctga cagaagaaga agtacatttg ttcaagaaga ctttggcatg
     1981 cttcagtaaa aataacggaa gcatcaaaaa ttgctcagaa gaattattta ttcataaaaa
     2041 caccttgcaa tatcgattga acaagttcca ttctctcact ggttataccc ctagaaacta
     2101 tgacgattat catattctaa agctggcttt tttgttggta caaacatgac aactacaaaa
     2161 aaaccaggcg aacatatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt
     2221 actagaaaaa ctacttccgc tttttcccgc caaaagcatc tttaaactta tcgaagaaac
     2281 cgtcttcttg ctgttcttca actttgatgc cgccagcttc agcgaatgca cgtaatgctt
     2341 ctctttgttg ttcatttaga tttttcggtg tgatcagttt tactttgaca tgttggtcgc
     2401 ctgttgcacc accacgtaac ttcggagcgc cttttccacg taaacggaag ttcgtacctg
     2461 tctgtgttcc agcagggatc ttcaatttca catctccatg gacagttggt acttggactt
     2521 catcccccaa agctgcttgt acaaaactta aaggcaattc atagtaaatt tcagaacctt
     2581 cacgatcaaa gatatcactt tcttctacac ggaagaccac atacaaatca ccaaatggtc
     2641 cgccgttttc tccagcttct ccttgaccag ctaaacgcat ttgttgtcct tcttctacac
     2701 cagctggtac attcacttta actgaatgtg ctttcttctc atgtcctgag ccatgacagg
     2761 ttggacaagg ttctttgatt tctttccctg tcccatgaca aacatcacaa gtttgacggc
     2821 tcatcactcg tccaagtggt gtttggcgtt ctacattaat cgtacctgca ccatgacatt
     2881 tatgacaagt agttggttgt gtgcctggtt ttgcaccgtt cccaccacaa gtatgacaga
     2941 tttcttcccg attatacttg atttcttttt ctacaccaaa aatcgcttct tcaaaagaca
     3001 agtcgaccgt atattgcaaa tcagcacctt gacgaggcgc atttggatcg actgaccgac
     3061 cgcctcctcc aaagaaagag tcgaagatat cttcaaaacc accgaagccg ccaccagaga
     3121 agccgccgaa tccaccaaat ccgccaccag ctccaccagc accgtaattt ggatctgtgc
     3181 ctgcatgccc gtattggtca tatgcagcgc gtttttgcgg gtcacttaaa atctcatatg
     3241 cttctgatac ttctttgaat ttttcttccg catcaggttc tttattgatg tcaggatggt
     3301 attttttcga aagcttacga taagctttct ttatttcatc ttctgatgct ccttttgaga
     3361 gccctaagac ttcataataa tcacgttttg ttgccatagg ctttcctcct attggttctc
     3421 atcattttca cgtcgcttgt tatcatatca tattttgttt gaaacaaaaa ccgatacttc
     3481 aaactatttt tcaaaagaaa aagccaaagt agcaactttg gccttctctc gaaattcagt
     3541 gaactgatta tttatcgtcg ccatcgactt cttcaaaatc agcatcaacg acatcatctg
     3601 ctccgccttg agctgcttct gggttttctt gagcttgttg ttgtgccgct tgttcgtata
     3661 gtttaacagt caagttttga acgatttcgt tcaatgcatc gcgtttagtt ttcatttctt
     3721 cgatgttgtt tgcttcaaca gcggctttca attcatcgcg agctgtttct gcttttttca
     3781 cttcttcttc gtccactttg ccttctaatt ctttcaatgt tttgtccaca gagaatagca
     3841 atgcatctac atcattacgt aggtcaactt cctctttacg agctttatct gcttctgcat
     3901 ttgcttctgc atcttttacc atacgttcga tttcttcgtc tgttagacct gaagaagatt
     3961 tgatcgtgat tttttgttct ttttgtgtac ctagatcttt cgcagataca tttacaatcc
     4021 cgtttttatc aatatcgaat gttacttcga tttgtggaat accacgagga gctgctggaa
     4081 tatctgtcaa ttggaatctt cctagagttt tgttgtcagc agccattggg cgttcacctt
     4141 gtaatacatg gatgtctact gctggttgat tatctgccgc agttgagaat acttgtgatt
     4201 tacttgttgg aatcgttgta ttgcggtcaa tcaatttagt gaacactcca cccattgttt
     4261 cgatacctaa tgacaatggt gtaacgtcaa gtaaaacaac atctttcaca tcaccagtga
     4321 tcacgccacc ttggatagca gcacccatag caactacttc atcagggtta actgatttgt
     4381 ttggttcttt tcctgtttct ttacgtactg cttctacaac tgcagggata cgagtagatc
     4441 caccgactaa aataacttcg tcgatttctg attgtgataa acctgcatct tttagcgctt
     4501 gacgtactgg tacttttgta cgttctacta agtcagctgt taattcatca aattttgcac
     4561 gtgtcaatgt catttccaaa tgcaatggtc cagattctcc tgctgtgata aatggcaagc
     4621 tgatttgtgt gcttgatacg ccagataaat cttttttcgc tttttcagca gcgtctttca
     4681 agcgttgtaa agccattttg tcttgagaca agtcaatgct gttttctttt ttgaattctg
     4741 ctaccatgta gtcgatgatt ttgttatcaa agtcgtcccc acctagatgg ttgtcgccag
     4801 ctgttgacaa tacatcaaag acaccgtctc ctagttcaag gatagatacg tcaaaagtac
     4861 cgccaccaag gtcgaatact aagatttttt cgtcgcgatc tgttttatct aaaccatagg
     4921 caagagcagc tgcagttggt tcattgacga tacgttctac ttctagacca gcgatcttac
     4981 cagcatcttt tgttgcttga cgttgtgcat cattaaagta agctggcaca gtgataactg
     5041 ctttttcgac tttttcacct aaatagtctt ctgcgaatcc tttgatatat tgaaggatca
     5101 ttgctgagat ttcttgtggt gtatatgatt taccttctac atctactttg taacctgctt
     5161 caccgatatg gcgtttgatt gatgaaatcg tatttgggtt tgtcacagct tgacgttttg
     5221 caacttcccc tacttggatt tctccatttt tgaatgatac aacagaaggt gttgtacggt
     5281 ttccttctgg gttagcgatg atttttgctt cgtttccttc caatactgct actgcagagt
     5341 tagtcgtacc taagtcaata ccgataattt tactcataat taacgtctcc ttctttaatt
     5401 gaaagtgtaa gttttatatc cgcacaatta ttgtgcaaca atgaccatgg ttggtcttaa
     5461 cacgcgatca tgtaatttgt agccttcttg tagtacttct acaatcgtat cggctggtgt
     5521 atcttcagtc gctggaacag tttgtactgc ttgatgaaga ttaggatcaa aggcttctcc
     5581 tttagcaggg attttttcaa ttccttcttc ttccagtgcg ttacgcaaac tttccaagac
     5641 catttccacg ccttttttca aacctgcccc ttgatcatct gacacttctg tggctaatgc
     5701 acgttccagg ttgtcaattg atggaagtaa ttttttggct aaatcttgtg aacggtattt
     5761 ttgtaattgc tcacgctcat tttttcctcg atttgccata tttgcgattt cagcacgagc
     5821 ccgcaagtat ttatcttcca tttcttctaa ctcagctttt aatttcgctg tttcgaattc
     5881 ttccgcttca gcttctgaaa tacccgaatc ttctgcagct tcgacatcga tttccggttc
     5941 tggctgagca tctgtcattt ctttatctag ttcttcttga ttttctttca cgatacagtc
     6001 ttcctttcat tattcagtca gctgtttgat tgctcttact aagaaagaga gaacgatcag
     6061 ctgcaaatag cttcgttatt cacattacga gtttgacaag tcaagcgaac ggtagtaatc
     6121 agcaagttta gctgctagtt ctcttcgata aacatcgact agaccgaaca tccgagaata
     6181 aggcatacta gtaggcccta acaaggctat tgtacctctt ccatgaccat ctatctcgta
     6241 acttgcttga atcatactca tattctgcaa taaatcattg cctaattcag caccaatctt
     6301 gatatgaata ttgctatcca tcggagtgat catttgtgta agtccgtctg agtctttcat
     6361 aaaagaaaac atcgatttga actgatcaag atcttgacgg ggctcataat ccaagagatt
     6421 catttgtcca ctgacataaa ccttatcttc aaaaacatga ccaagaacgg tgtcaaataa
     6481 ctgagacatc ccttctgtcg tttggaaata tttatgaaga atcatcggaa tctccgtacg
     6541 aagtttatgg tacaccgtca tcagaggatc tccgactaga cggtcgttta caatctgtac
     6601 cattttttct agttcctgac ttcccatata ttcgggaaga gtgaacactt ggctttctac
     6661 attccccata tctgttacga cgatagcaat aatctgtcga ttattcaagg gtacgatacg
     6721 aaagccagtc agtcggcggt cttttatttc aggtcccaat gaaaaagtcg tatagcttgt
     6781 cagctcagat aatatctcag cagattgctt aataatctca ttgatttcat gaaattctct
     6841 gcctagagac tgacgaatca gcagcaaatc atcttctgct acgcgagcag gttttaacaa
     6901 atgatccacg taataacgat agcctaaagc agatggaatc cgcccagaag aagagtgagt
     6961 cttcaacagc aatccttcat cttctaatgc tttcatatca ttgcgaatcg ttgctgagct
     7021 ggccttgact ccctcagaca tcaatgtttt ggaaccaacg ggcacgccac tactcgtata
     7081 gtgttggatg atcagacgta aaatgtcgtt ttgtcgcttt gttaacatct tcctcacctc
     7141 tcattagcac tcatatattc taagtgctaa tccacttata aatatagcaa gaagcccttc
     7201 tcctgtcaag agaaagactc cattttttag cactctaaac acaagagtgc taattctaaa
     7261 taattgatgt aaatccagtt atatcaacgt ttcaatagtc ctttattgta tttttgaact
     7321 ttttttattt aaattcagat aaaaaacaaa tattctttaa actatttttc aaacaaaaag
     7381 cgctcaaaca cattgttccc caaaaaggtc cctcttggtg tcaaataaac acggtcttct
     7441 tcattaacaa gtaacccttc ttgctctaat tcccttagca catcaccgta caccgattca
     7501 attgtttttt gatagcgatc atagaaatgt tttttagtaa tccctgtttt tttcttagtc
     7561 ctaagaacat ttcttcttct atttgatttt ttcgtgtcaa tttttcttct tccaataccg
     7621 ggcgttcatt tgctcgtaat gggcgtaaat agtgttggat tggtcctttg ttgcggtaac
     7681 gaatatctcc aagataaccg ctagcaccag ctccaaaccc aaaatagtgg tcattgttcc
     7741 agtacatgag gttatgttga gattcatgtc cggagagccc aaagttactt atttcatatt
     7801 ggtgacgtcc atgcttttcc attgcttcaa tcgtctcttc aaacattctg gtttccgttt
     7861 cctgatcagg cagttcgagt cttccttgtc gtacccagtt catgaacatt gttttgtttt
     7921 cgaggatcaa tgagtacaaa gaatagtgag gcagatccaa agccaaagct ttttccaaag
     7981 tatccagata tccttctagt gtttgtcccg gtaatgcata aatcaaatcg atgcttacat
     8041 tggtaaaatt ttctgcttct aaaaattgca tagtttgata cacatcttct gcggtgtgtt
     8101 tacgtccaat ttttttcaat aagcgattat caaatgtctg cactcccatg gataaacgat
     8161 tgacgccata gtttttcatt acttgaagtt tttcacgggt caaatcacct ggattcgctt
     8221 ctacagtaaa ctcattacca ttttgaaaag gcaggatctc tctagcacct gataacagtc
     8281 gatcaagttg tttagcagat aaagaagttg gtgtcccgcc ccctacgtaa agtgtttcta
     8341 aattcgctgt cggatgactt tctagcatta ttttcatttc attcagcagc atttccacat
     8401 actcatctac tggctgccct tctaaaaaaa ctttattaaa atcacagtaa tagcagatat
     8461 gctcgcaaaa tgggatatga atgtacgcag aaaccttgtt ttctgcggga tttattgttt
     8521 ctatcattga aattctttct ccttacgtcg aaaagtcgtg cctgttttcg accttcttaa
     8581 aatcgcgttc aagcaccaac tcattgaaat aagccgcata tttagaatca aggaaaccta
     8641 gctctaaaaa tgcttgctta ttgtcatcgg agctacacgg tgcttttaca gcctctcgat
     8701 aaacggtaat cgcaaagctg accttcggca ttaagtcaaa atctgcaaaa atatgaaaag
     8761 ctattttcac agattcctcc ttaagtgctc agaccacaac gctttgctta caacctctca
     8821 tatacgcata ctaaacgtat ttggcgaaca aatccactaa aaaatccaag aatcgacttc
     8881 ttggatgatt ttttctttca ttaaactggt tacctagtta tatatctcta gggttgtttt
     8941 catgtctgag tgcccgatat gatttatttt tcgtttctat aattactttt tgccttccag
     9001 tttcttttaa tattgtactc aagcaagtgg gaattggttc ttttgtcatc catgtatgca
     9061 aaatgttata atgctggtaa acaaaaatga ttacctgtca tttttattta gttcgtattt
     9121 aattaagttg actatctgta tactctgtct aaaacttaag aattgttgtt ttcgctgtcg
     9181 ctacccaatt ttaatgaatg tctgtaggtt aaaactgttg tccttgttat agatagtaaa
     9241 ggagagcttg aaaatgaaaa ctgcgattac gtttctatta gaggaatatg ccgattggga
     9301 aggtgcttat attactagca cactaaacca aaatgagaaa tggcaaaaca aaactgcttc
     9361 tatccaatca gaggtcactt ccattggagg attaaaaaca aaaatcgatt acttaatcga
     9421 acaaatacct gaaaaagttg acctttttat tatgatcggt ggcaattcat gggacataaa
     9481 aaatcatcat ttatatcaaa taatcgataa attgttaaaa acagacgtac cagtagctgc
     9541 tatttgtggg gcagttgatt atttagctca gagcggatta ttgcaaggtt acaaacacac
     9601 cggaaatgcg caatttcctt ggttagaatc ggaaaattat cagaataaag aagattttat
     9661 agaacaacaa gctgtgaaag atcgtaattt gattactgcg aatggaacag ctcctataga
     9721 attcacagac ttgattcttc aagcagtcca ttttgaaaca gaagaaataa gagaaaaaac
     9781 gattcgtcta tacaaacttg gattctatga ttactataaa aagtacggag atcctttctc
     9841 ctaatcattc tcttcaatta aatactcctt atcaatagtg tgacaagtaa ttttactgat
     9901 gattatttgt cacacttttt ctgacattta tcaa
//
LOCUS       DASNNU010000092         9860 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.092, whole genome shotgun sequence.
ACCESSION   DASNNU010000092 DASNNU010000000
VERSION     DASNNU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.092"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            254..733
                     /locus_tag="U8116_002259"
     CDS             254..733
                     /locus_tag="U8116_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435217.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            766..2994
                     /locus_tag="U8116_002260"
     CDS             766..2994
                     /locus_tag="U8116_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN8435218.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3123..4766
                     /locus_tag="U8116_002261"
     CDS             3123..4766
                     /locus_tag="U8116_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN8435219.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4839..5384)
                     /locus_tag="U8116_002262"
     CDS             complement(4839..5384)
                     /locus_tag="U8116_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEN8435220.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5407..5892)
                     /locus_tag="U8116_002263"
     CDS             complement(5407..5892)
                     /locus_tag="U8116_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN8435221.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5882..6694)
                     /locus_tag="U8116_002264"
     CDS             complement(5882..6694)
                     /locus_tag="U8116_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEN8435222.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6833..8365)
                     /locus_tag="U8116_002265"
     CDS             complement(6833..8365)
                     /locus_tag="U8116_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEN8435223.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8474..9505)
                     /locus_tag="U8116_002266"
     CDS             complement(8474..9505)
                     /locus_tag="U8116_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN8435224.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3284 a         1835 c         1900 g         2841 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      781 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      841 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      901 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      961 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     1021 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     1081 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1141 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1201 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     1261 gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc
     1321 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1381 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1441 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1501 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1561 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1621 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1681 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1741 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1801 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1861 gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1921 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1981 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     2041 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     2101 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2161 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2221 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2281 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2341 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2401 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2461 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2521 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2581 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2641 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2701 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2761 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2821 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2881 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2941 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     3001 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     3061 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3121 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3181 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3241 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3301 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3361 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3421 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3481 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3541 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3601 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3661 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3721 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3781 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3841 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3901 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3961 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg
     4021 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     4081 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4141 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4201 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4261 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4321 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4381 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4441 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4501 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4561 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4621 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4681 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4741 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4801 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4861 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4921 cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata
     4981 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     5041 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     5101 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5161 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5221 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5281 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5341 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5401 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5461 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5521 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5581 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5641 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5701 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5761 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5821 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5881 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5941 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     6001 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     6061 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6121 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6181 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6241 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6301 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6361 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6421 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6481 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
     6541 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6601 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6661 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6721 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6781 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6841 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6901 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6961 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     7021 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     7081 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7141 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7201 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7261 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7321 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7381 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7441 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7501 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7561 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7621 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7681 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7741 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7801 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7861 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7921 gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca
     7981 ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata
     8041 cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag
     8101 acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt
     8161 ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc
     8221 attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga
     8281 taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag
     8341 gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa
     8401 attcaagata atcgtaacat aatcctttat ataataacat agtttattgt gaaaaaaaga
     8461 tcatacattt gttttatatg gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa
     8521 aatcaaccat cttttcccag atatcttgaa aagaatggga tgagaggtat cgattaccac
     8581 ataaataatt taccgcgtca gcatcatcta aatatagttc tgcataataa cgatatgttt
     8641 ttctgcatag ttctggcgat acgtttttat atactgtaat tttgctcaaa atttggcgaa
     8701 tagtccgagg agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg
     8761 tattttgtgc tgttaactta aaatagttct ccagaaatgg catcgcttct tttggtattg
     8821 gtactttatt cagtctgttt tcttttctat tcagattaag aatctgcttg gtcaaagata
     8881 catctttacg tttaagttct actacttcat ggatctttaa tccgcaatac agaagaatgg
     8941 cagtaattgc aaggttcctc ggtctattct tttcttcaaa gctagcttct ctgcttgttt
     9001 gaaagccttc aatcgggata aacaaatgct caaaatcttt gaaaagttct gcatcgatat
     9061 atcgcggagc tgctttggtt gtttccggta aacttgtacc caaaagagaa atctcttctt
     9121 caatcagtcc tttttgatac aaaaatcgta aaaaatgatt taatgaatat tgcttccttg
     9181 aaatcccgta atagctattt tcaaacaatt gctccgtttc ttggttcgct gtagtttgga
     9241 acgaacgagt atgtcgatag attttagcta agaaattctg ataatcttgt ttttctaaat
     9301 ggataatcag ctgatcatcc acttcttcag aaaatacttt tctcaaataa tcagtaaaca
     9361 gatgaaaatc ttttgcgtat tcatagcgac tgcctaatga aagggaagaa cgattttctt
     9421 ctaaaaactg ttgaaagacg gaagggagca agtgtgtacg ttgctttaac ttttcttgca
     9481 gttgttttct agttttgctg tccattacaa attccctcca atagtgttca ttttctatat
     9541 tttaccgcat ttcatcaaat gaagatagac agtttgtctt gatcttagga agcaaatttt
     9601 taactaagta ctagctaaaa gatatgaagt aagaagatac atttttagtt attttattag
     9661 tgcagtcatt cttttgatct tttaattgat ttatcctctt tttagctatg tggttacttc
     9721 atgagcaatg aaatggattt ggttcgttct aatggcagga ggctctttct tttatttccg
     9781 aaaaattaga aaacattttt tcagaatcga ttggctctcc atttattcaa taatggtttg
     9841 tctaacgata ttgttgggat
//
LOCUS       DASNNU010000093         9811 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.093, whole genome shotgun sequence.
ACCESSION   DASNNU010000093 DASNNU010000000
VERSION     DASNNU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.093"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(33..2153)
                     /locus_tag="U8116_002267"
     CDS             complement(33..2153)
                     /locus_tag="U8116_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN8435225.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2383..3561
                     /locus_tag="U8116_002268"
     CDS             2383..3561
                     /locus_tag="U8116_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEN8435226.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3577..4335
                     /gene="aroD"
                     /locus_tag="U8116_002269"
     CDS             3577..4335
                     /gene="aroD"
                     /locus_tag="U8116_002269"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEN8435227.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4358..4843
                     /gene="ybaK"
                     /locus_tag="U8116_002270"
     CDS             4358..4843
                     /gene="ybaK"
                     /locus_tag="U8116_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEN8435228.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4914..6167)
                     /locus_tag="U8116_002271"
     CDS             complement(4914..6167)
                     /locus_tag="U8116_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN8435229.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6284..7633
                     /gene="gdhA"
                     /locus_tag="U8116_002272"
     CDS             6284..7633
                     /gene="gdhA"
                     /locus_tag="U8116_002272"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEN8435230.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7745..9091
                     /locus_tag="U8116_002273"
     CDS             7745..9091
                     /locus_tag="U8116_002273"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEN8435231.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9399..9785)
                     /locus_tag="U8116_002274"
     CDS             complement(9399..9785)
                     /locus_tag="U8116_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN8435232.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3127 a         1782 c         2009 g         2893 t
ORIGIN      
        1 attttgtcgc tgctcctttt aaatttattt tatcattcgg aagaacttgt ggtcgttccg
       61 tgaagttctt ggtacgtttg cgttttataa agatcaacag ttgattgtcc gttgtgttca
      121 ctaaagacac tagttgactt gtcaccaaga gatttttcag cttcttttaa tttctctaag
      181 ccattcttgt agtcataatc actggtatca attgggtcta atccactatt cgtatagaac
      241 cgaagaagat caccattatt gatttggtca gaaacagcta actgtttatc aactttttct
      301 ttcagtgaat caatttcatt ctgtacttcc tcagtaggtt cagtaatagc ttcaccagtt
      361 tcgttactat agatcgtacc accgtagtaa gtatattttg gtgtaacaaa atcaccatca
      421 cggaaagaaa caagctgttc atgatctttc gagaatagat cttgtcctaa ctgaatataa
      481 ttttttgtat ctacacctag taagtgaagt aatgtaggca aggcatcaac ttgtccgccg
      541 taagtgtgat tgattccgcc ctgtgtttgt ccagggatat ggatcatata agggacacgc
      601 tgcatttgtg cattgtcgta gtttgtccaa gtagaacttg tttttccaac taattcagct
      661 agatttttat ttcttgaatt cgagacaccg taatggtcac cgtaaaggac gatgactgaa
      721 ttatcataca agccgctttc tttcaaataa ttgaagaatt cttgtacggc ggtatctaaa
      781 taattggctg tcgcaaagta accgttgatc gtttcatcat ttgtattggc taacgggaat
      841 cctgcttcgt cattggtgaa ttttgaataa ggataatggt tagatacagc aatgaatttt
      901 gaataaaatg gttgttgcag acgttccaag tattgtacgg attgttggaa aaatggttta
      961 tcatgcaacc cgtattggaa cgaattgtca tcattaacat cataataaga agaatcaaag
     1021 aaataatcat atcctaaatt cttgtatgtc tcgtttcgat tccagaatgt accagcgttc
     1081 ccatggaata ccgcactaat gtaaccttgt gtttgactta aaatatcagg tgcagcttgg
     1141 aaagtatttt ttccgcctaa ttgtgtgaac aaggagcctt gattcaagcc aaacaatgaa
     1201 ttttccatca gcgtttccgc atcgcttgtt tttcctgctt ttacttgatg gaaaaagttg
     1261 tcaaaactga acgtgctgtt gctgtggtaa agactattga tgaaaggtgt aacttcatgc
     1321 tggagaccat tctcatctgt caatttataa tcgatcagga attgctggaa gctttctaaa
     1381 tggatataaa tcacattttt tcctctagcg agcccgaaca aatcgtcatt tggtgctgca
     1441 taatgttctt ttacataatt ttctacttct ttcatatcat tgggacttgc ttctgcccgc
     1501 acttggtttg tctggtaagt ttgaacaccg tcataaacca tgaaagcatt caagccaaga
     1561 tatttgacca aataatcacg agagaatgtt cgagtcagca attctggacg atcagcttcc
     1621 gccataaaaa gatttcctga gaaaatcata actgctaatg tagagatcgc aaaagccatt
     1681 cttgctcgga tcgggcgagg gtccaacttg attttttttg caatcagcag aacagtcaag
     1741 ataataaaat cgatccaata gatcacatca tatgggcgaa acagtcgaat ggcactttct
     1801 cccagaccac tcgctacttt cccagcacct aaaatcgtgt tgatcgtaat gaagtccgtg
     1861 aattctcgat aataagtgac attcgaaaaa acaagcaatg tcaaaagaaa ataaatgacc
     1921 atcatcgtga tataagcaac tctagggcgt ctgatataaa gagcaattcc aagtaataac
     1981 aatgtagttg caatagggtt gataaacaag ataaagtatt gaatgccact ttcgattccg
     2041 agatggaaat caactaaata ggcaaaaatc gttttcaacc aaagtaaaac gactagtaaa
     2101 gaaaaaaatc cgagcctctt attcaaaaag gcaggtgttt ttatacattt caagataatc
     2161 ttcctttctg cacttcttaa tcacagaagt gtcctgaaac catgttacac agctcactta
     2221 ttctcattta ttttactagt atgacaatat agtgtcaatt gaaaccagaa gtttatgctg
     2281 tttcattaag gttttcttta agcttcatga aaaaatgagc agtaagacaa gtcctatgtt
     2341 ttttgttata cttgggaaga gaaagctaaa gcaggtgaag gaatgaaagt aaaagtaaaa
     2401 aaaagagcaa gcaatcgatt gaaaaaagga tatccactga tccaaaaaga agaccttgct
     2461 catccccaaa aagaaaccac gcaatgggtg gaattagttg attcgcaaaa ccaatttatc
     2521 gcaaaaggat atctaggaaa gcagaataag ggaatcggtt gggtattgag ccagcagaac
     2581 atcccatttg atcaatcttt ttttgaagaa ttttttgtga agtcaaaaga aaaaagaact
     2641 ttcttttttt cagatgattc tactaccgct tttcgtatat tcaatggaga aggagacggg
     2701 atcggcggtc tgattgttga ttattatgca ggttatgctg ttttttcttg gtataacgaa
     2761 acgttatatg cacatcgtga attattgatt caagcttttc gtgaaatcta cccggaaatc
     2821 ataggaggct atgagaaaat tcgatttgaa tcagtaaatc tgcctgagag ccagttcatt
     2881 tacggaaaag aggcaccgga acccttgatc gtaaaagaaa acggagtaaa atttgccgtt
     2941 tatttaaatg aaggattaat gactgggatc tttttagacc aaaaagaagt acggggatca
     3001 ttagcagaag gtttcgcttc tgggaaaaca gttttgaaca tgtttagtta taccggcgcc
     3061 ttttctgttg ctgccgcaat gggaggggca atcgaaacaa caagtgtcga tctagcaaaa
     3121 cgcagtttaa aaaaaacaca ggaacagttt gaagtgaatg gtttggatgt cgatcagcaa
     3181 aaaatcattg ttatggacgt attcgagtat ttcaaatacg ccaaaagaaa acaattttta
     3241 tatgacatga ttgtattgga tccaccaagt tttgccagaa ataagaaaaa agtctttcgc
     3301 gtagcaaaag attatgggga actggtcaag gatagtctgg atattttagc agaagacggg
     3361 atacttattg cgtctacaaa tgctgcgaat gtctcgatag aacaattcca aacaatgatt
     3421 gaagaggaat tccatgccaa aaatgtttct tttgaacaac ttgctttata tcgtctgcca
     3481 aaagatttcc agacgacgga taccttttta gaaggcaatt atctgaaagt gtttgtttat
     3541 caaattaaaa ataaccatta gaaggagcga tgcagaatgg ttcgggtaaa gggattgaat
     3601 ataggaagcg gtagaccaaa gatttgtgcg cctcttatcg gggccgtccg agaggaagta
     3661 ttacgtgaag cagttcttgc acgtgaagca ggggccgatt tagtcgaatg gcgagtcgat
     3721 cattatcgtg aagttttttc ttttgatgaa gtagcagaaa cattggttct tatccatgag
     3781 atgcttgatg gactgccgtt attatttacg tttcgtacct tagctgaagg cggagaacaa
     3841 gacatatcga tccgtcagta tcgcgacctt tatgaacatg tgatacacac aggattagta
     3901 gatatggtcg atatcgagtt gttcaaagta gaaagtctag gaaaaagact gatagaggaa
     3961 atcaagtctt taaacattcc tttaattatc agtagtcatg attttaaaga aacaccagca
     4021 gaccctgtct tgctttaccg attgaatgtt atggaacatt ttggagccga tatcggaaaa
     4081 ctggcagtaa ctcccaataa tgaacgagat gttttgcggt tgatggagtt gactagacgg
     4141 gcaaatgctt ttttctccat gcctattatt acgatgtcca tgggaaatct tggtaaaatt
     4201 tctcgtctgg ttggcggaac gacgggttcc gttatgacat ttggttcctt aagcagcgat
     4261 tctacttctg ctccaggaca attaccggtc aaagcattga agcaaattat tcaattatta
     4321 aatgaaccgt tgtagaacaa gtttgaggtg atattttttg gcaaagaaaa aaatagtaaa
     4381 aacgaatgca atccgtatgg tcgaacaaaa aaagattccc tatacagagc acgaatatga
     4441 gtgggacgaa agccacctaa gtgcgtcatc ggtcgctgaa cagcttccag aaagccaatc
     4501 tcgtatattt aaaacactgg ttgctgtggg caatgtgacg gggcctcttg ttgctgtgat
     4561 accgggagaa gccgaattga atctaaaaaa attagcaaaa gtttcaggga ataaaaaagt
     4621 agaaatgctc catttaaaag atttggaagc aacgaccgga tacatacgtg gtggctgctc
     4681 gcctataggt atgaaaaaac tatttcctac ttatttagat caaattgcag aaagctatga
     4741 acaaatcatt gtctcggctg gcagaagagg gctacagatg gagttagcac ctcaagatat
     4801 ctgtgcatta acgagcggac aatttgcaga tataaaacaa taaatcatac gtataaaaga
     4861 aagaaggagg ccgggatgtt gtttgtccag tctccttctt tcttttttat acattatgct
     4921 ctttgcccgg cattcattcc tgctacatgc cctgtagcga aagccgctgt gatattaaag
     4981 ccgccagtat aaccattgac atccagtaat tctcccgcaa aatacagacc atcaatgatt
     5041 ttactctcca atgttttagg ataaatttcc tttagagaaa cccctccgcc agttacaaat
     5101 gatttctcaa taggaaaagt tttgttgatt gatagcttaa actctttgca tagttgaaca
     5161 aaatcttgaa tttgtttttg agaggcttgt tgtcctgtca gatgatccat ttcaagccgc
     5221 tcaagaaaga aaactaacaa tcgctcaggc aatataccat gccaagcatt gaccaagttc
     5281 tttttggttg ctttactttt ttctgtcaat atatgaataa gctcttgttt ggtttgtgta
     5341 gggaaacaat ccagagaaag tgtcactggt tctcccgttt tctctaattc tttattgaca
     5401 aaacttgaac aacgcagtgc agccggacca gaaataccga aatgggtgaa aagtaaatcc
     5461 atcgtatgtt cagtcaatac acgacctttt ttatttaaaa cgcgtaaagt gatgtcttgt
     5521 aaagaaagcc cttgaagtgt tttctcctga atatacggtt catccgatat caagggggac
     5581 tctgtcgcat aaagaggcgt caccgtgtga cccactcgct tcacgatctt gtacccatct
     5641 ccagtagaac cagtagatgg ataagtccgt ccaccagttg ttaagataat ggcaggagca
     5701 tggaactctt cgaattccgt gcgtataccg taaattttat gttctttgtg gatcaatttc
     5761 tctactcgag tagagaatag aagcgtcaca tctagttctt tcagacgatt gagcaatgct
     5821 tcgataatcg tttttgattt atttgttaca ggaaacatcc gtccatgatc ctcttctttt
     5881 aaatgtacgc cttgtgattc aaagaagttc atgatatcga aattattcca ttgcgcaaac
     5941 gtactatata aaaactttcc atttccagga atatgtgcaa tcaaatcatc aacaggacga
     6001 ttgttcgtaa cattgcaccg accaccacct gtcatcagta gtttttttcc tgcttttttg
     6061 tttttttcga tcaagagtac gcgtgcgcct tgttcagctg cactgatcgc tgccatcatc
     6121 ccgcttgttc cagcgccgac tacgatcaca tcatacagtt tttccattcc ctcacctcaa
     6181 ggaaagtgta gcatatctga agaaagaaaa cagaaaaaaa tgttttgagc caaaagagaa
     6241 aatgagctaa aatgagggaa gataagcaaa ggagcgtgga acgatgacaa acgcaacaga
     6301 atatgttcaa gcaattcaag aaaagctaca tcaaaaggat caagggcaag aagaattttt
     6361 acaagcaatc gatgaattca tgcccacggt tatgtctttt ttggacactc atcccgaata
     6421 tatcgaaaag aatatcttag aacttttaac ggaaccagaa agagttattc aatttcgagt
     6481 gccttggcaa gatgatgaag gaaactggcg agtaaataga gggtaccgta tccaatacaa
     6541 ctcagctatc ggcccttata aaggtggatt gcgcttccat cctagtgtca atctaagtat
     6601 cttgaaattt ttggcttttg aacagatttt caagaatagt ttaaccggtc tgccaatcgg
     6661 tggaggaaaa ggcggaagtg acttcgatcc taaaggaaaa tctgataatg agatcatgcg
     6721 tttttgtcaa agtttcatgc tggaattgtc aaaacatatc ggcccatcga ttgatgttcc
     6781 tgcgggagat atcggtgtag gagctagaga aatcggctat ttgttcggcg aatataaacg
     6841 attgaaaaaa tatgatactg gtgtgttgac aggtaaacct ttggattttt ggggaagtaa
     6901 gatacgtacg gaagctactg gttatggatt agtgtattat gtcaaacatc tattgaacga
     6961 agaaaaagat tcatttaatc aaaaaacagt ttttgtttcc ggaagcggga atgtagccat
     7021 ttatgcaatc gaaaaagtgc aagagctagg cgggaaagtt gttacatgtt cagattctag
     7081 cgggtacatc tatgatccag aaggaataga tgtgaaacta ttgaaagaag tcaaagaaat
     7141 caatagacaa cgtttgacgg aatacgcaaa ccaacgtcct tctgctattt atcatgctgg
     7201 agaatctgtt tggacattga aagaaaaagc ggatatcgct ttgccatgtg ctactcaaaa
     7261 tgaaatagac gaaagcttgg ccgaaatctt agtagaaaat gggatcaaaa tcgtagcaga
     7321 aggcgctaat atgccatgta cagtaaaagc tgtttctgtt cttcatgaag caggcgtgtg
     7381 gtattgtcca ggaaaagctg ctaatgctgg aggagtagca gtatctgctt tagaaatgag
     7441 tcagaacgtc cagcgattga cttgggaaag agagcaagtg gatgagcagc tggatcaaat
     7501 catggaaacg atttaccata cgtgtcgcca aacagcagcc gagtacggcg acgaaaaaaa
     7561 tctgcttctt ggagcaaatg ttgctggatt tgaaaaagtt gccaaagcaa tggcagcaga
     7621 aggtttagtt tgaaaaatga aactttcttg gaaatattgt cagcattttg tttatttgct
     7681 tgaaaaataa taaaaaaaaa cgtaaagtat tacctgtaga gtaaacctat aaggaggaga
     7741 acaaatggca cacattcatt ttgactattc aaaagtagca ccgtttgtca acgaacaaga
     7801 gttagggtac atgcaaagcc aagtaacagc agcccacaac gaattacggg aaggtactgg
     7861 cgcaggaagc gacttccgcg gctggatcga tttaccaacg aactatgaca aagaagaatt
     7921 tgcacgtatc aaagaagcag ctaaaaaaat ccaatctgat tctgaagtat tagttgttat
     7981 cggtatcggt ggttcttatt taggcgctcg tgcagcgatc gacttcttga atcacacatt
     8041 cttcaattta ttagatagca acacaagaaa agcaccacaa atcttctttg ccggaaattc
     8101 aatcagctca acatatattg ctgacttgat cgaagtaatc ggtgatcgcg acttttcagt
     8161 aaacgttatt tcaaaatctg gaacaactac tgaaccagcg attgcgttcc gtgtattcaa
     8221 agagcttctt gttaagaaat atggacaaga agaagcaaac aaacgtatct acgctacaac
     8281 agataaagca aaaggtgcag ttaaagtaga agcagatgca gaaggttggg aaactttcgt
     8341 gattcctgat gacatcggcg gacgtttctc tgtattaact gctgttggtt tgttaccaat
     8401 cgctgttagt ggtgcagata tcgatgcatt gatgcaaggt gccgctgatg caagcaaagc
     8461 atactcaagc gacaaactag aagaaaacga agcttaccaa tacgcagcaa tgcgcaacat
     8521 tctttaccgt aaaggcaaag taactgagtt gttgatcaac tatgaaccag gaatgcaata
     8581 cttctcagaa tggtggaaac aattgtatgg tgaatctgaa ggaaaagacc aaaaaggcat
     8641 ctatccttca agcgcaaact tctcaactga tttgcactct ttaggacaat tcatccaaga
     8701 aggacgtcgc aatatttttg aaacagttgt gaaggttgaa aaacctcgta aatcaatcac
     8761 aattcctgaa caagcagaag atttagacgg tctaggctac ttgcaaggaa aagaagtgga
     8821 cttcgtaaat acaaaagcat ttgaaggaac attgcttgct catacagacg gtgacgtgcc
     8881 taacttgcta gtcaaaattc cagaaatgga tgcttataca ttgggttata caatgtactt
     8941 ctttgaaatt gctgtaggta tttcaggtta cttgaatgga atcaacccat tcgaccaacc
     9001 aggtgtagaa gcttacaaga aaaacatgtt cgcacttcta ggcaaaccag gctttgaaga
     9061 tcttgctaaa gaattgaacg aacgcctgta agataagggt ttttcgctag atttaaataa
     9121 tcagtgatag aaacatgttc gctttaaaat gaaaactggt acccatttag gtactcaaac
     9181 atactgcatc gtttattcag aataaggggc tgtgacaaga cgtttgtcac agtcccttgt
     9241 ttgtatagaa atcgattgat tgccttgctc tttagtttgt tttaggactt taagaacagt
     9301 gaggctgact tcgaaggtta attctgtttt tttgttttct agaatcagta agcaaaaaag
     9361 cactcacttc gagtgctttt ttgcgatata ttcttagctt aagataggcc taatttttct
     9421 tttacagatg agggtacctt atcgctggta attacttttt tctctatgat ccgcttttcg
     9481 cttgctttca actgcaagat cgttccttcg gggtaagcag aaccattggc agagtcacca
     9541 gaagtaatga catcgaaatc taactgtctt ttttcaccgt tttcagcata agcatctacg
     9601 ttgtatttaa aacctcttcc aattacttcc ccattatctg ctttgatatc agtttctgca
     9661 ggcattggag ctttgactac cgcataataa tctttaccta cataagtaga ctgatagtag
     9721 tcccaacctt tccatcctat aaaagcaatc aatatgataa cgatcaaccc taaaattttt
     9781 ttcatgaaag atctcctttt ttatttttat t
//
LOCUS       DASNNU010000094         9797 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.094, whole genome shotgun sequence.
ACCESSION   DASNNU010000094 DASNNU010000000
VERSION     DASNNU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.094"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            291..1430
                     /locus_tag="U8116_002275"
     CDS             291..1430
                     /locus_tag="U8116_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEN8435233.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="U8116_002276"
     CDS             complement(1497..2072)
                     /locus_tag="U8116_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN8435234.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="U8116_002277"
     CDS             complement(2077..3051)
                     /locus_tag="U8116_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEN8435235.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="U8116_002278"
     CDS             3334..4302
                     /locus_tag="U8116_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN8435236.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="U8116_002279"
     CDS             4415..4693
                     /locus_tag="U8116_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN8435237.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="U8116_002280"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="U8116_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEN8435238.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="U8116_002281"
     CDS             5150..5905
                     /locus_tag="U8116_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN8435239.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="U8116_002282"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="U8116_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEN8435240.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="U8116_002283"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="U8116_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEN8435241.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="U8116_002284"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="U8116_002284"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEN8435242.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
BASE COUNT         3262 a         1729 c         2099 g         2707 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actacta
//
LOCUS       DASNNU010000095         9700 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.095, whole genome shotgun sequence.
ACCESSION   DASNNU010000095 DASNNU010000000
VERSION     DASNNU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.095"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            283..522
                     /locus_tag="U8116_002285"
     CDS             283..522
                     /locus_tag="U8116_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435243.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="U8116_002286"
     CDS             complement(884..1144)
                     /locus_tag="U8116_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435244.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="U8116_002287"
     CDS             complement(1329..1826)
                     /locus_tag="U8116_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEN8435245.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="U8116_002288"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="U8116_002288"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEN8435246.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="U8116_002289"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="U8116_002289"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEN8435247.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5210)
                     /locus_tag="U8116_002290"
     CDS             complement(4548..5210)
                     /locus_tag="U8116_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN8435248.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5220..6035)
                     /locus_tag="U8116_002291"
     CDS             complement(5220..6035)
                     /locus_tag="U8116_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN8435249.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6297..6740)
                     /locus_tag="U8116_002292"
     CDS             complement(6297..6740)
                     /locus_tag="U8116_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEN8435250.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6874..7212)
                     /locus_tag="U8116_002293"
     CDS             complement(6874..7212)
                     /locus_tag="U8116_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN8435251.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7200..7577)
                     /locus_tag="U8116_002294"
     CDS             complement(7200..7577)
                     /locus_tag="U8116_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN8435252.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7801..8994
                     /locus_tag="U8116_002295"
     CDS             7801..8994
                     /locus_tag="U8116_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN8435253.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9160..9582)
                     /locus_tag="U8116_002296"
     CDS             complement(9160..9582)
                     /locus_tag="U8116_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8435254.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2891 a         1937 c         1590 g         3282 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa
     5041 agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct
     5101 gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac
     5161 caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc
     5221 tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat
     5281 ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca
     5341 tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac
     5401 aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa
     5461 ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat
     5521 aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta gtattgcttc
     5581 aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatat
     5641 gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca
     5701 gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa
     5761 aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa
     5821 aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc
     5881 tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct
     5941 ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc
     6001 agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt
     6061 tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat
     6121 ccagaacaac ggttattata gctgattcat ttccgttctt cttatgtttg taagcgctta
     6181 tttaacaagt tatgtaaatc tctttatttt ttttgattta aaaatgaaaa aatacaaaag
     6241 caagactgtc atacgtctcg cttttgtatt gaaatttctt cgattagaga tacttattac
     6301 tgttcaaaag cttctttgaa acggtgaatc aattcattga ctacaggtga taaaatccga
     6361 ttctttttcc agactagcat gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc
     6421 actcgctttg aaagatgact tacagcacct tctatgacta aagcagcacc tacctgattc
     6481 tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc
     6541 ttttttatgc ctaaccagtc agcaaacatt cgctgtattt ctggacgctt tgagatcaaa
     6601 agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt tttttgctaa aacagaattt
     6661 tctaaaatga gcaatcccca tttttccttt cttggtaaca aaatcgtttc gtatttctct
     6721 gtatctactg gatctagcaa aatcgctaaa tctaacaggc ttttttctag tcactccgtg
     6781 atatcatcac ttgttccgct gtggatatga aattgaacgc tgggataatc acggatcaac
     6841 ttttctagta atagtgccaa tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat
     6901 cggccgctga ataattgttt ttttctgttc tcgaattctt gagtggtttg attcgttaaa
     6961 tctaaaattt cttctgctcg gcttttttag aaaataacct gcttctgtca agatcatttt
     7021 gcggttttcg cgtacaaaca gttctgttcc tagttcctct tctaactcct tcagttgtcg
     7081 gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct tgagagattg ttccttcttc
     7141 tgcaattgtc caaaaatatt ttagtaagcg taactccata gcgatgctcc ttgatttttt
     7201 tacttgatac attccatttt atttctagat aacacacgat catatttctt gatttttcct
     7261 tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt tcgtcaaaag ttcactttct
     7321 tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc cttcgctaga taggcgaata
     7381 tattcaatca aagactcgat cgataatccg gcatttcgca tacagatcgt aaagtcgacc
     7441 cattttaggt cttcttctgt ataatcacgg attccgcctt ttgtccgatt aactggtgga
     7501 atcaacccga ttcgttcata atagcgcaat gtatcggccg aaatgccctt ttcttcactg
     7561 acttgtttga tattcatctg atcactcttt ctcttaattt cttgccactt tacttatgta
     7621 ttttatttat tctacaactt taagtgtact ccaagtcaat actcatttct attaaaacta
     7681 agctggatta tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt
     7741 tattcttttt tggatatact tttaacaatt tagggaaatt ttaggaaaag gggtataaaa
     7801 atgacaacgt atcagcgaaa ggtattatta tcgacatcgg cgggaattgc tttagagaat
     7861 atggatatta tgtttttagc tttttcatta tcttcaatga ttacttcttt tcatattagt
     7921 ggtacgcaag cgggtttcat ttcaacgatc actaatttgg ggatgctcat tggaggtatt
     7981 ttttttggca tcatagctga taaatatgga cgtgtgaaag tattttctca aacggtcctt
     8041 ctgttttcga ttgcttcttt attgatgtat tttgcttcaa acatctactt ggtttatctc
     8101 tttcgcttta tcgcaggtgt aggagcggga ggagaatacg gggcgtgtat gtccttgatt
     8161 tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt cggttgctgg tattggtgcg
     8221 caggtcggtg cggctttggc tgcgatttta gctgctgtga tcattccaac gtttggttgg
     8281 aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc
     8341 ttgactgaac caaaggaatt caaatctgta caatctgccg aaaagaaaac caaacttagc
     8401 agactgtttg aatcaaaaca tcttgcttgg caaacatgtg gccttagtat catggtaact
     8461 gtccaaattg ccggctattt tgggttaatg aattggctgc cttcaattat gcaaaatcga
     8521 ttagggcttt cggtttctgg gtcttcattg tggatggtca gtactatttt aggcatgtct
     8581 ttggggatga tgacttttgg cataatcatg gataaattgg ggcctaaact agccttttct
     8641 ttgtttttaa tttgttcatc cttatctgtc tatctacttg tgttagccca tagccagtgg
     8701 agtttggtag cagccgctgt gatagtcggc tactttatca atggcatgta tggtggatat
     8761 ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa taatttcatt
     8821 atgaaccttg gacgagctgt tggtggtttt tcttctatcg tcatcggttt cttgatggat
     8881 cattatagtt taactgccgt aatcttgttt ttgagcggta tttacctagt cagtctgctg
     8941 gtcttgacaa ctcttactgg tgttacagca ttaagggaaa gcttgcagca ttgatagttg
     9001 agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc atctgcggta ataagaccag
     9061 ccaaatcaaa aacatgagac actgtttccg tttggctggt cgtattactt gatgcagggc
     9121 agctttttca actgctaatt tccttctatc tggcggcgac tattctttga cgtccgtcat
     9181 ttcaaagtac tttactaata acttagcata acgaaaaact gtggatacac tcgtatattg
     9241 agcgtctgca taatcttcca aatttttctc aggatgttga tacatgaatt caatcaaatg
     9301 ataattatat gattttctgt agaaatgaag ataaataatg tagattggga agttctcctg
     9361 cttttccata gaaaagcgcc gattattctc attcttcaaa atgaccttat caggatattt
     9421 ttggaacaat tcttctaaat ctgttagata attcaagata gacttacttg ataccttcaa
     9481 ttcgttttgt agttcttcta ttgaacacca acgttctgtt tcaagcagta aatgaatcat
     9541 tttaattttc ttttgagaaa cttttgttaa aaaagtctgc atattttctc ctcctgtatc
     9601 tactttacta tactacatac aagaaaaaaa acggatacaa atatcatatt gtcacaccgt
     9661 caatagtgca taaacctgtt attatttctc tttttttcct
//
LOCUS       DASNNU010000096         9677 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.096, whole genome shotgun sequence.
ACCESSION   DASNNU010000096 DASNNU010000000
VERSION     DASNNU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.096"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            18..497
                     /locus_tag="U8116_002297"
     CDS             18..497
                     /locus_tag="U8116_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN8435255.1"
                     /translation="MVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITNDLKLSTSMIHL
                     IDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNKKTKLVSLTKY
                     GRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEYQVSLTRKED"
     gene            590..1752
                     /locus_tag="U8116_002298"
     CDS             join(590..866,866..1752)
                     /locus_tag="U8116_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN8435256.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2032..3012
                     /locus_tag="U8116_002299"
     CDS             2032..3012
                     /locus_tag="U8116_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN8435257.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3134..4552
                     /locus_tag="U8116_002300"
     CDS             3134..4552
                     /locus_tag="U8116_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEN8435258.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4623..6092
                     /locus_tag="U8116_002301"
     CDS             4623..6092
                     /locus_tag="U8116_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN8435259.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6102..6569
                     /locus_tag="U8116_002302"
     CDS             6102..6569
                     /locus_tag="U8116_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN8435260.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6605..7477
                     /locus_tag="U8116_002303"
     CDS             6605..7477
                     /locus_tag="U8116_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN8435261.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7559..>9677
                     /locus_tag="U8116_002304"
     CDS             7559..>9677
                     /locus_tag="U8116_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN8435262.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGN"
BASE COUNT         3363 a         1410 c         1943 g         2961 t
ORIGIN      
        1 tagaaagagg tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca
       61 tcaaagtaaa taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc
      121 taaaactcag tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta
      181 gtattacgga attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc
      241 ccactttaga gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta
      301 aacttgtatc gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg
      361 aagagcttta tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa
      421 ccattaaaca gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga
      481 caagaaagga agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca
      541 gaaaaaataa ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg
      601 tcaacgtaga acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg
      661 aaagccccga gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg
      721 ggtcaagcaa gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca
      781 agtagaacta attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt
      841 aaagcaagcg gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa
      901 tactcaatat cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa
      961 gccaaaccag ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc
     1021 aatcgaaaag cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa
     1081 gtgagtcaac gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca
     1141 ttggctcatt tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta
     1201 gacggaacct tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt
     1261 cgtgttggga agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc
     1321 atcggctatt catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag
     1381 attccgtact cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat
     1441 aaccagacga ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa
     1501 ggctgcccct atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc
     1561 gttcatcaat accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc
     1621 cattggtgga ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt
     1681 cgacagcaga gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc
     1741 gaggcacctt aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg
     1801 ttgtcaaggg cagtcggaac accagcagca agcttacgca ataccttata atttttgtca
     1861 aaaaaagtgt tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg
     1921 atattatata tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat
     1981 gtcgaatagt cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa
     2041 attggaatta gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta
     2101 ttgaatgatc gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa
     2161 aaattagaat actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc
     2221 gtaggtttga tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc
     2281 gagttcgagc tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca
     2341 acaaaagaaa aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta
     2401 ggaacgcaca ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt
     2461 gatagaagtc ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa
     2521 ttagctactc aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat
     2581 agtaagataa agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa
     2641 ttcatgctgg aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat
     2701 gaaataatta agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat
     2761 gatttattgg cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa
     2821 gaggttaaag tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta
     2881 tcaactattc aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat
     2941 atgattaagg gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa
     3001 aatacagcat aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga
     3061 aacgggttga catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca
     3121 ctggaggaag aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca
     3181 acctaaggat gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat
     3241 ttatcatcaa cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt
     3301 agcaactacg aaagattttg ttcattttga agactttggg gaatctcttt taagagggga
     3361 tgacaatgag agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt
     3421 tcacgcattt tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt
     3481 gttgcttcat gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga
     3541 gttagaacca caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga
     3601 tgatgaaaaa gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct
     3661 acaaaccgga cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg
     3721 tgatttttgg gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg
     3781 tgactggtgg tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat
     3841 gagtcaaagt ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc
     3901 ttattatgca ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc
     3961 aacaaaagat caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca
     4021 tgaagtctat caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa
     4081 tgcattcata aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga
     4141 agaagtaacg cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt
     4201 tgatgagaat cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc
     4261 ttacgaatat cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta
     4321 cccttggttt caatatatgg ataaaggact tattagacct atatctttaa agcctaacca
     4381 atcttataaa ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat
     4441 cgctcttaac gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa
     4501 tggatcaatg actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc
     4561 aatcatgtgg caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat
     4621 gaatggacta caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata
     4681 tctcaagcgc tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag
     4741 cgaatatgga aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac
     4801 agttacaagt aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat
     4861 tgacaggagc taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag
     4921 gaaatagact tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag
     4981 cttttattgg ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt
     5041 ttggaatgga tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc
     5101 tgggtattat tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa
     5161 aacgttttgg cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag
     5221 gactagccaa tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat
     5281 ttggattaag cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt
     5341 cttggttttt agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt
     5401 acattttagt cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg
     5461 gtccattggg gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga
     5521 agatgggtgc ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag
     5581 gaacacaaca tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt
     5641 atatccaacc tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt
     5701 atttactctt taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa
     5761 cactggtagg tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc
     5821 cttttgtttg ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg
     5881 taaaagccat tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc
     5941 ttccttatat atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt
     6001 atgataaata taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg
     6061 actcaaaaaa attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa
     6121 aaaatgaaat aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa
     6181 gtgatccagt cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg
     6241 aaacgattgt atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct
     6301 taggaataaa aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg
     6361 agctaaatgg tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg
     6421 atattcttat gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca
     6481 ttgttgtttt tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac
     6541 aaggacaaga attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa
     6601 atgtatggtt tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga
     6661 taaagatttg aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat
     6721 gaatttagtt ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg
     6781 atcgttcggt cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac
     6841 gccaaaactt gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat
     6901 tccaactgct tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa
     6961 tggcagaggc ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc
     7021 tatccaaaac ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt
     7081 aaaacgacac aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga
     7141 gggcttagct gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac
     7201 tgaaaatgac gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac
     7261 aacattgatg ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca
     7321 cttaaaaaag aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc
     7381 cgatgttgac aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg
     7441 tttagcttta gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac
     7501 tatttgtttg gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt
     7561 gaaaaaaaaa atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga
     7621 tttttctgct caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta
     7681 tttgaatcag ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat
     7741 gttcaagtat aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat
     7801 atctttgaaa tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct
     7861 cccattaata ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta
     7921 tcagtatgct cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg
     7981 tgcagattat gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg
     8041 agcctttact ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata
     8101 ccttttttta gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct
     8161 tatggattct caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt
     8221 tcgccttatc ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag
     8281 acgcatccca gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat
     8341 tcaattatta tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg
     8401 ttcgattgaa ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt
     8461 gaggaatgaa atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc
     8521 aatttattta ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga
     8581 tttaaaaaaa atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa
     8641 tcacatttca attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc
     8701 aggttcttct gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt
     8761 tgttaataaa atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt
     8821 ggcggatatc agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt
     8881 tttgctgtct aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa
     8941 tgagatttgg agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat
     9001 actttttctt gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat
     9061 gcacggtaaa ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata
     9121 tgtttatgag gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat
     9181 caacaatttt tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg
     9241 atcattggaa caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt
     9301 aaacaatgtt tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc
     9361 aattagtcat ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa
     9421 aggattatca caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga
     9481 aaaagttaag gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga
     9541 ttttaacgca ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat
     9601 ttgtgttctt tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa
     9661 tctaattaat gggaatc
//
LOCUS       DASNNU010000097         9055 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.097, whole genome shotgun sequence.
ACCESSION   DASNNU010000097 DASNNU010000000
VERSION     DASNNU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.097"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(133..306)
                     /locus_tag="U8116_002305"
     CDS             complement(133..306)
                     /locus_tag="U8116_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN8435263.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="U8116_002306"
                     /pseudo
     CDS             754..3116
                     /locus_tag="U8116_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="U8116_002307"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="U8116_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEN8435264.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="U8116_002308"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="U8116_002308"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEN8435265.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="U8116_002309"
     CDS             5704..6489
                     /locus_tag="U8116_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN8435266.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="U8116_002310"
     CDS             6502..7353
                     /locus_tag="U8116_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEN8435267.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="U8116_002311"
     CDS             complement(7477..7929)
                     /locus_tag="U8116_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8435268.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="U8116_002312"
     CDS             complement(8394..8762)
                     /locus_tag="U8116_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEN8435269.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..>9055
                     /locus_tag="U8116_002313"
     CDS             8912..>9055
                     /locus_tag="U8116_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEN8435270.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFL"
BASE COUNT         3061 a         1514 c         1870 g         2610 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tctta
//
LOCUS       DASNNU010000098         8861 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.098, whole genome shotgun sequence.
ACCESSION   DASNNU010000098 DASNNU010000000
VERSION     DASNNU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.098"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            13..312
                     /locus_tag="U8116_002314"
     CDS             13..312
                     /locus_tag="U8116_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435271.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(409..843)
                     /locus_tag="U8116_002315"
     CDS             complement(409..843)
                     /locus_tag="U8116_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8435272.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1075..1785)
                     /locus_tag="U8116_002316"
     CDS             complement(1075..1785)
                     /locus_tag="U8116_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN8435273.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1775..2635)
                     /locus_tag="U8116_002317"
     CDS             complement(1775..2635)
                     /locus_tag="U8116_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEN8435274.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2640..3278)
                     /locus_tag="U8116_002318"
     CDS             complement(2640..3278)
                     /locus_tag="U8116_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEN8435275.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3293..3592)
                     /locus_tag="U8116_002319"
     CDS             complement(3293..3592)
                     /locus_tag="U8116_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN8435276.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3635..5119)
                     /locus_tag="U8116_002320"
     CDS             complement(3635..5119)
                     /locus_tag="U8116_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN8435277.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5139..5600)
                     /locus_tag="U8116_002321"
     CDS             complement(5139..5600)
                     /locus_tag="U8116_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN8435278.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5618..6685)
                     /gene="ulaG"
                     /locus_tag="U8116_002322"
     CDS             complement(5618..6685)
                     /gene="ulaG"
                     /locus_tag="U8116_002322"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEN8435279.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6903..7673
                     /locus_tag="U8116_002323"
     CDS             6903..7673
                     /locus_tag="U8116_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN8435280.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7946..8773)
                     /locus_tag="U8116_002324"
     CDS             complement(7946..8773)
                     /locus_tag="U8116_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8435281.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2694 a         1865 c         1389 g         2913 t
ORIGIN      
        1 aggaaataaa acatggatta tactgttgaa tttgacagtg aactacttaa gcaatttcca
       61 caagaagaac atttcatgta taaagaagca atttcccaca aagtgacaga gtatttggag
      121 cataattttt ttcgtataaa gccagtgcgt actcgttcgg caaaaggaat atacgagatg
      181 aaagtcaaaa ttaataaaaa aaattatcgc atagcatttg ctttaaaaaa aaatcatgtg
      241 ttcatttttt atattagttc gaatttgcaa aaaattgtat ttgataaaga ggtgagtaaa
      301 aaaataaaat agctgagcat tggaggatag attgagtgat actaagtcta tcctcttgtt
      361 tttctaaaga actaagctag aattttttct tttgtacgtt attatatgtt atttgtcatt
      421 accaaaaagc accttttttc ttctagtatc tatgacataa gaaataccac ccatttcctt
      481 tacttgaaca aatcctactg attcataaaa tttttttgtt ttctcagtat catctgtcaa
      541 taattcgatt tggactggtc cataactagt gatcttctcc aaagcttcat tcaaaagcac
      601 tttgcctatt ccttggcgct gatacctcgg atgaatgagg atatcttgaa taaatgcgat
      661 cgtcgcaaaa tcagtgatcc aacgaacgat tccgataatc ttcccttccc cattacgctt
      721 tatcagagat tcagattgtt caaatgctct agctaacctt gcagtgtctt ttgtgtaatt
      781 cgtccaacca acgctcttat ataattcaga aatctcatcg atcataaggg aatcaatatt
      841 catttttttc ttcctttctt atacaattct ccctctaata atacagttct ttgactattt
      901 aatctatcac atataactct cctgtaaaga ttctctcgat tccttaaaat tcctctttcc
      961 ttttagaatg catacttttt gataaataaa aaggttgtga taaattttct tcacaacctc
     1021 tgtttctctc tatcaagtgt tatcttttaa ttttctaccc ttaccctagc atctctattg
     1081 cccgtagtat gcatactttc catgtttgcg aaaataatgt ttatctagta aatattgcgg
     1141 gatttcacat atattggggt tgatcagctc gttttctttg gccatcagac aaatttcatc
     1201 taagatcaaa ctgttttcta ctgctttcat aggtgaatcc ccccatgtga acggtccatg
     1261 tccatagaca agtactccag ggacttcgtt tggatctaat tttctttcct taaacgtctc
     1321 gacaatcaca ttccctgtat gaacttcgta agcttctctt acttcttctt tcgttaattg
     1381 gcgtgtacaa ggaaccttac cataaaaagc atcagcatga gtcgtgccat aagctggcaa
     1441 atcgcgtcct gcttgcgccc acatgacgga gtgcgtggaa tgagtatgag taatagcagt
     1501 tatatcttca aaagtttgat acaaaacaac atgtgtgggg agatctgaag atggttttaa
     1561 gctatcttct tctattatat ttcccgacaa atctgttacc accatttgat ctgcttgcat
     1621 gcattcatat ctgacaccgc ttggcttaat gacgatgact cctagctcac ggtccacttc
     1681 actcacattg ccccaagtca atttgactaa tccgtatcgc ggcaatgcca gatttgcttc
     1741 aaggacacgt tgcttcattg ctgcaatttt ttcttcacaa gacatcatag ccagcctcct
     1801 tcaatttaga acttaagtag tcttttgcct gctggatttc tcttcgaaag tcttggcttt
     1861 tttcactcca catttcaatc agaaatgttc catcataatc tagacgtttc aaatgtctta
     1921 ataatccaag aaaatcaaca caaccgctac caaaaggaac atcacgaaac tggcctgaat
     1981 attcttttgt tacagggtaa gtatctttta agtggatagc agtgatataa tcgatccctt
     2041 tctcaagttc tcgagccgga ttattttccg gccatgcgga caaattgcct aggtctggat
     2101 atacttgtag aaaaggtgaa tgaatctgtt ctttgatttc taaaaatttt gaaatggagt
     2161 tcataaatgg atcatccatg atttccacgg aaagaatgat gccatattta gaagcttcct
     2221 ttacgctttc ttttattcct tcgataaagt cttctctgga attcattgat ttctcttcat
     2281 aataaacatc gtatccagct atttggatta ctttgacgct tagattatgc gccaaatgga
     2341 ttgctttttt cattagttgt ttcgctgctt ttttcttttc tggatcagca gaaccaaacg
     2401 gaaatcgccg atgcgcactt aaacaaatcg aatggatacg tacaccggag ctgaacatct
     2461 cttctctcag ctcagctatc ttttcttcag accaatctaa tcgtgctaac cgctcatccg
     2521 tctcatcaat cgacatctcg ataaaatcga agtccatttc ttttacaagg gaaaaacgtt
     2581 ctttccatga aatatctttg ggtaacgctt tttcataaat cccaatttga gccatttcct
     2641 tacccccaga tacgaatgat ctcatctttg aagttttgtg cggccttcat cggatcattt
     2701 gctgctgtaa taccacgacc agtgataaag gtatacacat cgatcccttc aaataagtgt
     2761 aatgtgtgtg gatttaatcc accagttaca gaaacattaa agcctagttc aatcagtttt
     2821 ttgatcttcg ataaatcttt ttcaccccaa gtttcaccag ataacagcgc atcacggctt
     2881 tgatggtaga ttacctgccg gataccgatg ttatgccaat ccatggcttg ttcaaaagtc
     2941 caattgccgt agagttctac ttgcaattca cctatttctt tttgtgctgc ctccatagtt
     3001 ggaatcgttg cacaacaaat gactgtcatc cagtctgctc ctgcttttgc aacattactc
     3061 gccactgttc ctccagcgtc tgcacatttc gtatcagcga ctagtttttt atctgggaac
     3121 atactgcgaa tacagcgaat cgctttttgt ccttcctgta agcaaagaat tgtgcccact
     3181 tcaataatat ctactacatc accaactttc ataacatctc ctaatgcatg ttctaagtca
     3241 ttgtgatcta acgcaatttg caaattcggc ctgctcatct tagttccttc ctttacttgt
     3301 ttttttggta ccactttgtt tcagcaaatt tttcactgat ttctttgact gacatcacat
     3361 ttttgatacc gataactgta actccttttt tctctgcctg agtaaacata ttcaaaaaat
     3421 tgactggcgt aaaaactaca tcgtattggc cagctgaact ttttccctct gaaatggcac
     3481 aatgatggat tttggtaatt ggtacgttta gttctttcat tgctttttcc acacttctca
     3541 tcatcatcaa gcttgttcct gatccatttg cacacgatac taaaattctc atcttaatcc
     3601 ctccattatg agtgttgtct aatcagattt tcttttatac tctctgcttt tctgcatatg
     3661 tgtcatagtc ttctacaatc aaaaaataac catcaggatt tttccgatat tgtaactgcg
     3721 gaatagccaa taaaccaatg acaactacgg caattccagc ataacctagg actttcatga
     3781 ttactgtaaa caatggccaa gctgttgccc agtcgaacat cccgatataa ccaccgtatg
     3841 ttgacatccc aatccatgta gcgaataatg ctgaaccgcc tacttgtatg attcctgaaa
     3901 gaaacgggaa caggcaagcg gctttgaatc cacctcggtt attggcataa actgctataa
     3961 cggcattatc aaagaacaac ggaatgaaac cggctatgac gatcgttggt gatttcaata
     4021 aaatcagtaa accaatcatc aaaaactgtc ccaaagcacc aaacaagacc ccaatcgtta
     4081 ccgcatttgg cgaaccaaag ccaaaagtag cagctacgtc aattcctgga acagctcctg
     4141 gtaaaatcgt gtttgagatt ccttggaaag attgagttag ttcatcgacg aatgtccgaa
     4201 cacctaattg caagatcgcc agatataccg caaaatttaa agaagtcgtc ataatgtaga
     4261 ataagaaact ctgcccttct tgcatgaatt gcgcttgaat caaataatct ttgcctagta
     4321 caatcagaat gatcccaaag aaaaatagca tgagtaacga agtagcaacc atattctcat
     4381 tgaaaatcga caagaatcct ggtaatttga tatcttcaat ttttttgtct tgcttgattg
     4441 atctattttt cttttcatcc cgctttttca tccactcagc taattttgca aatataaaga
     4501 ttccgaacat ttgctgatgt gccactgcaa atccggcacc ttctgtcaat tcctgcgtga
     4561 ttcctactgt taagttagaa gctactgtcc aataacaacc taaaatcagt cccataaaaa
     4621 tcaatgcttg tatcctacct agatcaggaa agcaaaacag taaaatccaa aaagcggtag
     4681 ctgcttgttg aacctgaacg tttccggtag tgaaaacgga acgcagtttc gtatatttct
     4741 gaaaacggac taataagata tttaaaacaa atgcaatcag taataaaatc atcacatctc
     4801 caaaggtacg gccaaatgtc tccattaatc cagcatccac agcattttgt ccaaaataag
     4861 gatctgtaac catggcatcc aaattgaacc gttcctttag tccaaccaag attggccgaa
     4921 aattattgac caaaccacca gagccaacag tcaaaataaa gtaaccgacc gttgccttta
     4981 agaatccagc gatgcattca tgaagcgggc gtttcagcaa aatatagcct aataacacga
     5041 taaaaccaat caggtatgct ggctgcgtca agatatttgt tgcaaaatac gtccaaatgc
     5101 ttaacaaaaa ttcccccatc ttttttctcc tctatcaatt aattatattc tgccatcaca
     5161 acttggtaat catcgatgga gttcgtagag atgagacgct caaccaatcc atctgtcatt
     5221 aataattcgg ataatttttg gatattttct agatgttctt ctggatcttt tgctgcgagt
     5281 gtaaaaaaca aactagccgt tttctcctca ttctcccctt cactttcaaa acggattggc
     5341 tgtttcaact tggtaaatga aatagctgtt ccaaaaacac catcactttt ttctgaagaa
     5401 tgcggcattg ctacttgagg tgcgatcaca atataaggac cgtatttttc tacacaatgg
     5461 atgatctcct ccacatacgt ttcatcaata atttccttat cgatcaatac aagacagctt
     5521 tcggcgatag cttcacgcca atccgtgatt tctttatctg ggtaatgaat caattgatgg
     5581 tcaaaaaagt atttcagcaa gaaactcccc ttctttctta taaaaacgac ggaaacggtg
     5641 tatcattttc aatcgagaag acatcttcaa atcctcgata aaaattaaac tcaagatcat
     5701 ctttgtttgt cggatagatg aatttcccgc ctacttgcca gatataaggt ttaaatccat
     5761 acttcaggcg atcctttttg aatttccaaa tttcagtgat ctcttttgga tcagccataa
     5821 aattagacca aatatcgtag tggacaggaa tcactacttt tgcattcaat gactccgcca
     5881 tgcgcagcat atcaacagaa gtaactttgt ctgttatccc tcgtgggttt tcaccatagg
     5941 cccctaaaca gacatcgatt ttgtgttcat taccatgttt ggcaaataaa ttggagtaat
     6001 gtgaatctcc tgcatgataa agattccctc ctgatgtttc gaataaatag tttactgcaa
     6061 tttgatccat ctcctgaggc atttttcctt ttaatacctc ttctggatct tcacaagtga
     6121 ccaacgctgt ccgatcaaac gcttctaaag ctactacatt gatgtctttg attttgactg
     6181 catcacctgg ctttactgta atcgtttttt cttcaggaat tccccatttt tgccacgtgc
     6241 gaaccacttc ttttggccca atgaatgttg cttcaggaca attttgatga actgctgcgg
     6301 ctgtatggat atctagatga tcggaatgga tatgtgtaac gattaaagcg tctacatttt
     6361 ttatcgcaaa aggatcgatt acgaaaggct gagtacgtaa attaggctgc atattttgaa
     6421 cgccactcat acgcatcatc tgatggcctt ttttcatcat gccattccca tgactacgtt
     6481 ttcccgtccc acaccataga tcacataaaa tattcgttcc ttcatgactt ttcagccaaa
     6541 ttcccgtaca gcccagccac cacatagcaa aagttccagg atccactact tctgtttcga
     6601 tttcttcatt caagtatgtg ccccactcag gaaatgtttc tactaaccac ttttcttttg
     6661 ttacttcatt gacagtttta ctcatttgct tgctcccccc atatcgttgt tccaacttac
     6721 aagtaaatat taatgtgtat cagcatccat gaaaaccctt ttatatgacc gtttgttttt
     6781 tatatgattt tctttcagta atcaatgatt gtttcagtat aataaagaca gatatgaatg
     6841 tttaaatgaa caactagtca aataaatcca tggatcaaga tgtccctcag gaggcaaaag
     6901 tcatgaaaaa ctcacttgtc aatattgaaa aacgtcaaaa agaaattttg gaactgcttc
     6961 agcagactga acaactatca actttcgaat tagctgaatc ccttgatgta tctattagta
     7021 cgatacgaag agatttaaat ttattagaag aaaaaaacga gatcatacgc aattacggtt
     7081 attgttctta taattacaaa aaccagactg attttgatca atctggtcct gaaaggatta
     7141 aacaagcgat tgccagagaa gctagtaaat acattaacga ttatgatacg ctgtttatca
     7201 attctagctc gactgcgttg aaaacactgg attttcttca atctaaccat ttgacagtca
     7261 ttacaaacaa cgtgaaaatc aattactctc cgcacaaaga taattgtagt tatattttaa
     7321 ctggtggaga gatgcgcttt ccaaaagaag cactagtggg cgacatcgca ttaaatacga
     7381 tttcttcagt caatgcagac atttgtatca ttggctgtag cggagtcgat gtagtacatg
     7441 gggtcacaac aaagatatta aatgaatcaa cgatcaacga aatgatggtc aacaagacaa
     7501 tcaaatgtaa gattttagtc gctgatcatc gaaaaatcgg actgacatca aagttcaaga
     7561 ttgctgatat ttcagtcttt gattatttga tcactgataa attttgttct gaaaaagtat
     7621 tagaggagat cagaaatacg gggataaaaa ccattcaagt agattcattt taagaaccag
     7681 gacgtaaata tgtaaatagg attatcaaaa aaatgaataa caaatttatc gataaatgaa
     7741 aaacacgcta cgctcaaatt gataaaggaa tcccaagatg tatccatcca atcgatacca
     7801 tcataatcta ttgcttcttt tataaccgtt atatgttgcc taaataggaa tcaaacaata
     7861 tcgaaaaaag gaggtatgac aaaagtcaca cctccttttt tcgaataaac ggtattcaaa
     7921 tcagaacact gatttcacaa ccactttatt tcaggtcgtt ttcaatcacc tttaccgcgt
     7981 aatcatttcc gttgaaacgc gctatttttt ttgcgtccat atatctttct aaataatcat
     8041 cttctcctaa ttgcttatgg caagaagcaa tttcataaag caagtattcc aatgcataag
     8101 tcgatttatg tttcttatta tactcaatac ccttctcaca taattggatt gctttatcat
     8161 aatcctcgat tttacggtaa taggtggcga tatcataata tacaatgcag accatcagat
     8221 tatcaaaatc agtctcactc aatattttaa gtgtacgctc cacataaact ttcgcaaaat
     8281 ctaattctcc ttttgacaag tagatcattg ccacgtttgc agctgcttgt aagtgatgga
     8341 tatctgtttc ggtcgtttgt tccaacactt gattaaaata aaataatgct gcatctggtt
     8401 ttttcgcata aagatgatca agcagtcctt gataaaataa aatttgattt aaaagatcag
     8461 aattacttac tttttcctga tcgatcgttt gtaaaatttt ataagattct tggtattttg
     8521 atacagaatt caatgattga atctcattta tttttttcct gattttgtct tcttctgttt
     8581 ctactaatat atcggagatc tcgacattca gctttttgca aagcgcactt aagatattga
     8641 tgcctggcga catattcttg ttttcgatat tgctgacagt tgcttgttca caaatgcctt
     8701 ctgctaattg tacttgtgtt aacccctttt tctttcggta ttcttttagt aatgctccat
     8761 ctactttaat caaaacaatt tccttccctt taaaaaatta tttctccttt atatcataca
     8821 caaaaaaaag ttctaaacaa ccaaaaatgt ttagaacaga a
//
LOCUS       DASNNU010000099         8710 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.099, whole genome shotgun sequence.
ACCESSION   DASNNU010000099 DASNNU010000000
VERSION     DASNNU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.099"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            226..1920
                     /locus_tag="U8116_002325"
     CDS             226..1920
                     /locus_tag="U8116_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEN8435282.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            2608..4485
                     /gene="ltrA"
                     /locus_tag="U8116_002326"
     CDS             2608..4485
                     /gene="ltrA"
                     /locus_tag="U8116_002326"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN8435283.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            4616..4993
                     /locus_tag="U8116_002327"
                     /pseudo
     CDS             4616..4993
                     /locus_tag="U8116_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            4993..6198
                     /locus_tag="U8116_002328"
     CDS             4993..6198
                     /locus_tag="U8116_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN8435284.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            6214..6858
                     /locus_tag="U8116_002329"
     CDS             6214..6858
                     /locus_tag="U8116_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435285.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            6869..7675
                     /locus_tag="U8116_002330"
     CDS             6869..7675
                     /locus_tag="U8116_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435286.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            7697..7927
                     /locus_tag="U8116_002331"
     CDS             7697..7927
                     /locus_tag="U8116_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435287.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            8473..>8710
                     /locus_tag="U8116_002332"
     CDS             8473..>8710
                     /locus_tag="U8116_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435288.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYI"
BASE COUNT         3218 a         1374 c         1868 g         2250 t
ORIGIN      
        1 ccactctaca aaaaagaatt gatcaagatt ctgtatcgaa tgaacaatat gaatgatgag
       61 taggaatgga gaagagagaa tgaaatatcc gttgatatgg ttagaaaaag atgttccgaa
      121 ggaagagacc actagaaaat ttcctcacac tttaagcctg ggtaagttgg gaaaagggca
      181 atcttttaac caagtaaaga atcaaacaag aagaggtgac tgtacatgtt agaagcaatt
      241 aaaacagctt tttcaccaca aaatgatcaa aaagtacttg atccattaaa gcaactagga
      301 aatcgggaaa tagaaattgt aaaatccaaa gggtatgatt tagatttttt aaaacaagtg
      361 atgccacaag gtgggttaaa ttttgatgaa gaatatgtga cgtatggtaa tggttattca
      421 cgatgtgtga cagtttatca ctacacaaaa aatcctacgc ttttttggtt ggccaatctc
      481 atgaacaatc ctcatacagt ggccacactc gatgttagca cagatgaaaa gcaaaaagtg
      541 attgatcaaa tcaatcgtgc cttagatgaa ttaaatgatc gatcagataa tgaacgaaaa
      601 gcgactgacc gaaataaaag cgcggaagaa tacctggaac tagcggaata tgctagaaaa
      661 ttgacttcta atggggaaat ttctaagcaa gtcaaagtga gagtacatgt ttacggaaac
      721 acgattcaag aagtagatga acgagcgcag gaactgatcg aaaatctcaa agggatggat
      781 tatcgtgcgg tgacttatct gcataaaacc aaacgagatt ggcaatcgat gtttaccatg
      841 ttcgatgaac aagctgaatg gttcggaaat atcaaagggc aatcactacc atcgttaaac
      901 attggcggag gtttcccgtt ccaccaccaa acgctaaaag atccaggagg gacacaatat
      961 ggggaaacca tgacaggcgg cccatttgtc tggaatgcga cgtataaaaa tagtgtacgg
     1021 ttgtctacta ataccttgct tctaggaatg atgggagctg gtaaatcgac cgttcttaaa
     1081 atgatcgcgg aagcccattt agctgcagga gactttattt ggggctttga aaagggaaaa
     1141 gactttattc ctttcttaaa ggaatataac ggtattatgg tgcgtttaga cggatctgac
     1201 ggaatgatca atcctttaga gatatttgcc acacggacgt atgatgaagc atccagttta
     1261 tatgacgatg gatcagtcaa agatttgaag atcaatgaag cagcgtccta tcaaacacac
     1321 ttagacaaag tggtctatca ggtacagtta gtgagtcctc agctaaaagg aacgatgaaa
     1381 gcagaattta agacctactt aaatcgtttt tatgaagagt atggaacagt accacgcggc
     1441 tttacttcct caaattcgcg ttcaaatact gatacgcaag tcacagggaa agatccagaa
     1501 gcttatccga cgttcaaaga atttttagac tttttaggac aactggaatt accaggtgct
     1561 tcccaagaga aaaagaatcg aaaagaagaa atggaatcca tcgtagaatc cttatgtgag
     1621 acgtatggca tgatttttga tggtcatagt acgattcgac accttgacca acaacagttg
     1681 gtcttttttg acattgactc tattacaggc ttagatcgag gggtacaaca atgtcaaatg
     1741 tacatggcta tgacgttgat ttggaatcag gcattgattc aaggaagaag acaacgacgt
     1801 ttaattaaag aaaaacaaat ggaagaacaa gacttcaaac gcttcttggc gatgattgat
     1861 gagtgccata atggtgcgac ctgttctgca gtgaaaagct gtgggttgca agtagtctaa
     1921 aaactattgt aataactctt ataacgagaa gtcaaatgag tgggtaacgc cacaaagggc
     1981 ttccactgat actccgacat gcaacaagta gtttatgcag accaacgttg tgtgaagctc
     2041 ggtgaagtcg gctgaaggat accgtaaggt aaaagaaata gtgaatacca cgaaagttct
     2101 ctagaggtaa agggtgtatc cgataagtcg ggtgtccaaa aaaacttaat atggtgagta
     2161 tgtacaaacg tactgacgaa ctagcgaatg tactgtccga aaggcatagc tcctagaaat
     2221 gggagagaca gtgagcaaaa aatcactcat tgtgctaact tgtggttgag taaaagtcgt
     2281 tgttatgaaa gaccatgctg cgttacaggc gtaggagaag ttagggggca aaagctagca
     2341 tctaagttaa ggcatgtgga tagatataag ggaacagggg aagccatcaa catggagagt
     2401 gcatactcga tgaagtggta gtaagaaata tgtgaatgac aaataactta ctttagtgat
     2461 ggtgaaagta gagtcacgac tgatgaaggt tctgtaatgg aactggagga acaggctcga
     2521 ttcgtctgta aaagtcatga aacccgttca accatgggat tctcgtatga ctaaaaaggt
     2581 cacgagccaa ttggagcgtg ataaaccatg acaaaagaaa caagtccaaa agataggaag
     2641 ttgcggcatg cagaatatta tggcatgacg gaaatcttcg ataagatata ctccaaaagt
     2701 ttgaagggag ataatttcaa gaatctaatg cagattatca tttctgataa caacattgtc
     2761 ctagcatatc gtaatatcaa gagaaatcac ggtagtgtga caagaggaga agatggagta
     2821 acgattaaag atattgaaaa tctcaaccgt aatgaattcg tagaaattgt taggaagcga
     2881 ttcgcacatt ataaccctcg gaaagtaagg cgtgtagaaa ttcctaaata taatggtaaa
     2941 ttaagaccgt tgggtattcc atcaatatgg gatagaatcg cgcagcaatg catcttacaa
     3001 gtattagaac caatatgtga agcaaaattc aataatcaca gctatggttt ccgcccaaat
     3061 agatcagcag aacacgcaat agcagattgt atggcccgaa tgaatcaaag tcacatggaa
     3121 tatgtggtag atgtagatat caaaggtttc ttcgacgaag tcaatcatac taaactcatg
     3181 agacaaatct ggtctatggg tatacgagac aaacagcttc tcttaatcat taggaaaatg
     3241 ttaagagcac ccgtagtact tccaaatggg aagttgatat acccaacaaa aggaacacca
     3301 caaggcggaa tacttagccc attacttgcg aacatcaatc tcaacgaatt tgactggtgg
     3361 atagcaaatc agtgggagga aaaagatgcc aaagagctaa aacatcattt taggaaaaat
     3421 ggagcttggg ataaaggtaa tatctatcac aagctcagaa agagtacaac tctaaaagaa
     3481 atatacctag tgagatacgc agatgatttt aaaattttct gtagaaatta taatgaagcg
     3541 aaaaaagttt tctatgcaac taaaatgtgg ttacaggagc gtctaaagtt accaatatct
     3601 gaagaaaagt caaaaatcac aaatctttca aaagaagaaa gtgaattttt aggctttacg
     3661 atgaaattag tgaccaaagg taacaaaaag gttgttaata cgcatatagg tacaaaagca
     3721 caaagcatga ttaaagtgaa actaaaaaaa caaatcaaac gaattcaaaa aagtcctaat
     3781 agcaaagata cctttatcta tattggagtc tataacagta tggttattgg gattcataat
     3841 tacttcaaaa ttgcgactca ttgtagtgta gcaatgcgaa aaattgcaag agaactcaga
     3901 ccaagttttg aaatacgact acgagaacaa ggaatctcaa aagaaggaca atacggaaag
     3961 tacgaaggat acaagcctta cctacggtca ggacaaatac gatatataag agggtttcca
     4021 atattaccag tagggtatat tcaacacaga catccgatgt gtaaaaaaag aaatattaat
     4081 aaatacaccg aagaaggtag ggaagccatt cataagaagc aacaaaccgt agaagaatgg
     4141 aagatatcgt ggttgtgtag caatccagtg cttagtaaga gagcaacgat tgaatttgcg
     4201 gacaatcgaa tttcaaaatt catcgctcaa aagggaagat gtgcagtaac aggtgaggag
     4261 cttatcttat ctgaaatgca ttgtcatcat attatccctt atcatgaaag caaaagtgat
     4321 agctatgaaa acctagtgat tgttactgag gaggttcatc gggtaataca cgctacacaa
     4381 agtgaaacga ttgaggaact tctaaaatat ctaaaattaa atccaaaaca aaaagaaaaa
     4441 ctcaatgagt tgagattaaa agttggtaac gaagaaatat cttaataaat atttgtagga
     4501 cgggaaaaca gaatgtcttt tatagacgag ggaacgccgt atgcggtgaa agtcgcacgt
     4561 acggtgtgga ctgggggaaa agacggagat aatatcaaac tcttacctat cagtatcgat
     4621 taatccagat tttattgaaa cggtaaatta tgtcacaaac tttcaacgag aaatgcgtaa
     4681 agtagaagca atgactgttt tagctacaca atccccacaa gaaatggtgc cagaaaatat
     4741 gtcttctgat aacttctcga aactgaagaa agtctttgaa ttttcttcta cgaagatctt
     4801 tatgcgtatg gatgaatcta ttttacgtca tatagaagga ttagtgggta aatcaatgac
     4861 gaactcagaa ttagaatcca ttcctcaaca aaatcaagga gacgctgtga tcaattttgg
     4921 ctcaaaagaa accattcgtg ttcattttag tccgaacaaa cgtcaattaa aattgtttga
     4981 tggaggaaaa taatggaaaa ccaaaatgaa agtttaatca agcagtatgt taagcgtcgt
     5041 gcaaagcggc gcttattttt gtggttgttt ggtacttctg caggtttgat tactattttg
     5101 atcactgtgt tcgtgaccct atttctaata cttgcggcgg gctcgattga taattcggat
     5161 tcagattcaa gcagtggtgg cgaagctttt acaggagaat attcggaagg cttaccgatt
     5221 tataaagaaa tcaaaggaag aggccctttt tctgatgaga ttgcgcaata tgccgtaggt
     5281 gcagcagtga aatataaatt attaccaagt gtgatccttt cgcaatacgg ttacgaatca
     5341 gcgtttggta catcggcgtc cgcaagaaat gatctcaact attttgggat tacttggttt
     5401 gatggttgtc tgtttcctaa aggaactgca cgaggaattg gtggcattga aggtggctgg
     5461 tacatgaagt ttcccaactc aaaagctgca ttttcctatt acggctttat ggtagccaca
     5521 caatccaatt ttaatgcttg tgtaggaaat aaaagccctg gcgcaagctt attgatctta
     5581 ggacgaggcg gttatgccgc tgcaggaatt acggaagata gtccgtatta tacaggttgt
     5641 atgtcaatta tcacttctaa taaattaacc gaatatgatg aattcgccat caaacattgg
     5701 ggtgaaggtg gcaataacaa cggcacgatt actggtgaat ggacgaatcc attccctggc
     5761 agcagtttag ataaaagttc cttcagcggt ggtcaattat ttggaacgaa tccaggcggt
     5821 gaattccgtc cgaacggctt ccatgatgga ttagattttg gttcagttga tcatccagga
     5881 agtgagattc atgcggttca tggcggaaaa gtcgtgtacg ttggcaatcc tggtatttca
     5941 ggtttgggtg cgtgtgtgat cgtgattaat tacgatggct tgaatatggt gtatcaagaa
     6001 tttgcaaata gtacaggaaa ttctcgagtg aaagtaggcg atcaagtgaa agttggtcaa
     6061 gtaattggga tacgagatac agcacacttg catttaggat ttactcgaat ggattggcgt
     6121 caagcacaag gacatgcatt cattgatgat ggtacatgga tcgatccgtt accatttttg
     6181 aatagcagta aaaaatagat caggagaaaa aatatggaaa ataaaacgtt aaaaatcgtc
     6241 ttaacagctg caattagcgt tagtttaatc ggcaatattg cgttgttttg gcagctgaaa
     6301 gaggaacaag cagcaaatac ctataaaatt gaagaagtgg ctaaaaacgc gtctgagaag
     6361 ctcaaaaaaa attcctctga ggcacaggcg aagatcgaca agttaaatca aaccatcaat
     6421 gagaaaaacc aagaaatcga tcagctgaag caacaagatg gcacaaaagc aaaagaggaa
     6481 gtaactgaag cacaaagaaa gacggcagaa gagttcggtc aattggccat tgataaatca
     6541 ttaagcagaa atgaattaac ggaaaaacta aaagatgtcg caacccaaga agtgatcgac
     6601 aaattaagtc ctacagacga tgatcaccaa catgatgatt acggaagtta ctcctttaca
     6661 ttaggtgacg tgcaaactta cgctcaaaca agcagtgtaa aagaggaaca aaattttgtc
     6721 gtatttgttg attatacaat cggcaatccg caattcaaag atgtcaaacc acaaaaaata
     6781 aaaggtggat taactgtcac tgaaaaacaa gtagatggtc aatggaaagt catagacttt
     6841 agttatttca ctcgttagga ggaagaaaat gagtaagaaa aaaggagaaa gacaacgttt
     6901 caaaaaacga aaaagttatc ttagatggct gggaatcttt atgattctcg gctttttgat
     6961 tcttgctagt ccgatttata cgaatcagaa ggtagatata ccagatgagg taagcacgaa
     7021 cgccttttta aacacgaaag agctgacaat ggtttcaaaa gaaaaaggag cgaaccagca
     7081 attagttttt gaatttgcga tcgatgatga agatcaaagc ttgttaaaag aactggctaa
     7141 tcttaattat cgcgtagaag taaagacagc taaaggggat tatcaagcaa tacgagccaa
     7201 agtgattaag gtgtctgatg attattttgc agtgaagcta acgcatgtac cagaagaata
     7261 tatcgccatg cgtttaacca ttattcctga aaaaattgat ccaaaagtgg atatgcaaga
     7321 accacaagat ctgatttttt atattcacga agataaggtc aaagatcacg tgaaagacga
     7381 agattatgaa caacatgcga tcaattacaa agtgaaagga tacaagaaag aacaaaaagc
     7441 agctcaaaaa caaatggaat ctttccaagc aaccattgat ttgaatgaag aattagtgaa
     7501 gaagctaaaa gatcaattgc cttatcaagt cgcagatgat caggaaaata ctgaaaacaa
     7561 gattaatagc taccagcaag aaattgaaac atcgaaaacg caactgaaag atcaagaaga
     7621 gatcattcaa aaacttcaag aaaaaattga tcgtatgaca aaagaaaaga tttagaagga
     7681 caggtggagt gaaaaaatga agcgaacaaa cttggctgac aaagatactt tcgctgagca
     7741 gaaagtattg attaaagcct atctcaaagt tgtaaaagct aaagaaatat gtcgagaagt
     7801 gaatttatct aacacaacaa tatctgagtt gaagaatggt cgaaagaatt tagatacctt
     7861 aaaattcaga tcatttgaag ctctatatgt agctgctaaa aaatatttta cagctgataa
     7921 caaataaaaa acaggagtat tttatgtgaa aaaagtatat aaaaaatgct tatgaatcag
     7981 ctatatcacc ttaaccattt gttaatgaga tgggagaaaa cattaaccac gattctaacg
     8041 caaacatcaa gcacataaaa catttaacac tcctcctttc tggctgaaga tttcaaccaa
     8101 acaggaagac agcgtatcaa ttatagcata cactttttct taaattcatt tttcatatga
     8161 caaaatattt ccttctaatc gacaaagtta gaaggatttt ttattgtcta aatacacaaa
     8221 taaccttaat ttttaaagag cataaggaaa cgtgatatca ctttctcacg aaaggtgtta
     8281 ggtaggctgt gcctaccacg tttctaagaa agataaatga tatcaaaatt ttggatttgt
     8341 gtgtagattt gtgggtagga tgaaagcgaa tgtgggcagt aaaataagcg tttgtgtgta
     8401 gcaaaatcaa aaaatgtgta gggatatgtg tagctacaca tatccctaca cataataaat
     8461 ttggaggaaa atatggactt attaattaga aatattgaag aaaagtatat taataaaatt
     8521 gacaaacgtt gtgaagaact tacggtcaag actggcaaaa aatggagtcg aaatcaatac
     8581 ctaaaagttt tgatcgaaaa tgattttgat cacgctcttc tgaattataa aaaagaccaa
     8641 tttgatcggt tgttagaaaa atttgtggat attcaaactt ataatacgaa aactttagaa
     8701 gaatatattg
//
LOCUS       DASNNU010000100         8603 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.100, whole genome shotgun sequence.
ACCESSION   DASNNU010000100 DASNNU010000000
VERSION     DASNNU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.100"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="U8116_002333"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="U8116_002333"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEN8435289.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="U8116_002334"
     CDS             complement(1098..2201)
                     /locus_tag="U8116_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN8435290.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3247)
                     /locus_tag="U8116_002335"
     CDS             complement(2291..3247)
                     /locus_tag="U8116_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEN8435291.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="U8116_002336"
     CDS             3528..5462
                     /locus_tag="U8116_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN8435292.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="U8116_002337"
     CDS             5919..6335
                     /locus_tag="U8116_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN8435293.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="U8116_002338"
     CDS             6349..6915
                     /locus_tag="U8116_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEN8435294.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="U8116_002339"
     CDS             6938..7924
                     /locus_tag="U8116_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN8435295.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="U8116_002340"
     CDS             complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="U8116_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN8435296.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2527 a         1657 c         1752 g         2667 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacgagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac ttgtggtaga ggtagtatct
     8581 gtactgtcac ttgaagctga tgt
//
LOCUS       DASNNU010000101         8587 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.101, whole genome shotgun sequence.
ACCESSION   DASNNU010000101 DASNNU010000000
VERSION     DASNNU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.101"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(185..2566)
                     /locus_tag="U8116_002341"
     CDS             complement(185..2566)
                     /locus_tag="U8116_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN8435297.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2556..3203)
                     /gene="recU"
                     /locus_tag="U8116_002342"
     CDS             complement(2556..3203)
                     /gene="recU"
                     /locus_tag="U8116_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEN8435298.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3267..3806
                     /locus_tag="U8116_002343"
     CDS             3267..3806
                     /locus_tag="U8116_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEN8435299.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3896..4315
                     /gene="gpsB"
                     /locus_tag="U8116_002344"
     CDS             3896..4315
                     /gene="gpsB"
                     /locus_tag="U8116_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEN8435300.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4337..4704
                     /gene="rnpB"
                     /locus_tag="U8116_002345"
     ncRNA           4337..4704
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U8116_002345"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4913..6079
                     /locus_tag="U8116_002346"
     CDS             4913..6079
                     /locus_tag="U8116_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEN8435301.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6125..7621
                     /locus_tag="U8116_002347"
     CDS             6125..7621
                     /locus_tag="U8116_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEN8435302.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7702..8385
                     /locus_tag="U8116_002348"
     CDS             7702..8385
                     /locus_tag="U8116_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN8435303.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2691 a         1565 c         1826 g         2505 t
ORIGIN      
        1 tatgtaatga taaaagaaaa atggattttc taatttttaa gctaaatgtt agatcacaca
       61 tgtatatgct ggatagtctt ataaaaaatc atgtaataca gcttggtaca tgatttttat
      121 ttgtagctgt tttttacata gacacaaaaa agaaaccacg cttgtagacg tggtttcttt
      181 tttactatct gtttccagaa gctgaggtgc tgctggatgg tgtatggtca tttgcagcat
      241 ccgctccgct agttgctggc tcactggaag aagcaggagg agcagatgaa gacggtgtcg
      301 agctttcttc ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg cttgtttctg
      361 ctgtcgtgct ttcttttgag gactcagcag ttgattcgct ttgactagta gagctagagc
      421 tttgagtcgt tgtctctgtt gttgagcttc caggagtctg gacatagctg gaagatggaa
      481 tagttgttga tggtgtaatt gcattcgatc tagctgtata ttgatctttg tagtacagtt
      541 caccgcccac acgaattaat ccgcttggca tctcccagtc tgtatttgtc acgttagctg
      601 aaacatactg cataagctcg cgataaacat cagaggctac gtgtgacgat tctgaagtga
      661 caggcgtcat ttttttattg taacctgtcc acacggaaat cgaataattt ggcgtatagc
      721 ccgcaaatag gatatctgga tacaccccag aagaaatacc aagttttgca tactcgtcat
      781 ctgtgtaatt ggatgttccg gtcttaccgg cttgatataa cccagcgatt tgcgcattcg
      841 taccagttcc ttcagtgatc gtatctttta aaatgtctgt gatcatataa gctgtttctg
      901 gagacatggc tgtttttcca tctggctcat aagtttcttc tgttccgtct tggaagacga
      961 ttttattaac atattgaggt ttgtaataag tccctccatt ggcgaaagcc gcataggctg
     1021 ctgccatttt taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc gtattactag
     1081 agatcgcatt cgattgatgg atcgtactgt attcgatccc aagatttttt aagaaagaag
     1141 ccactttatc tgaaccgact tcattgaata atttgactgc tggaacatta cgggataagt
     1201 aaagtgcttg gcgtaaagta atggtgccca tatattgatt atcccagttt cctacaggag
     1261 tcgaagtccc ttcatagtta tacggtgcgt cagtaatagt ttttccagta gaatatttca
     1321 aatattcaaa agctggtccg taatctgtga caggtttcat cgtagaacca aaatcacggg
     1381 aagtattcac tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga cgtccgccaa
     1441 tctgggcttt tactttcccg gtattggtgt cgataagggt ggaggcaact tgcatctcgt
     1501 cgtctgggta agagacatac tgatccgtgt tgacgatatc atatagtttc ttttgggcgt
     1561 ccagatccaa attcgtataa atctctaaac cgtctgtgta gacattctta tctgtttttt
     1621 cttgtacttc attgatgact tctttgacat aattatcgac gatcttagta ttgtcatcgg
     1681 attgagttag ttcttgcaga ccatccgtta ccggtacatt aacagcttga tcatattctg
     1741 tttgagagat tttttcgttt tgaagcatcg tatacaaaac agtatctcgt cttttcttcg
     1801 cctgatctgg ataaacataa ggatcatagg cggaaggggc ttgaggcatc ccagcaagca
     1861 atgctgtttg cggtaaagaa agttctgata atttcttacc aaagtacatc tcagaagctg
     1921 tctccatccc gtacaagccg tttgacatat acactttatt tacgtagtag gtcaagatct
     1981 cttgtttcga ttttttttgt tccaatctta cagccatcca cgcttcttgg gccttgcgtt
     2041 tcagagtctg atcctcggca ctggtggaga agaaggaaag tttgatcaat tgctgtgtca
     2101 aagtacttcc gccttgcagt ccgccagagg taaagttaga gagggcagaa ccgatgatcc
     2161 gaatagggtc aaccccgatg tgtttataaa aacggcgatc ttctaccgac acgatcgcat
     2221 cttctaatgt tttcggtaat tcattcgcag aaattttttc acgcttttct gcgcctaaat
     2281 cttcgaatag ttcgccatct tgtgtatata gtttggaaga tacggtagcg tctaattttt
     2341 tatccgtcaa ttcgggcgca tcttttgcgt aataccagaa aagtcctaca cctgctaaaa
     2401 agaggataca gaaaaatgag agtattccta aaaggatctt gataaataat ccttttttat
     2461 gtgttccaga ggactttcca gaacctttgc cagaattctt cttcacactc tttttaggag
     2521 taggctgttt ctgtcgtcta gagcttctag tttgttcatt tgccattctt gctttctcct
     2581 tcaatagata atgttgataa atagaagtca accgcgtcca aataaggaat ccgcggagaa
     2641 ataccagttt tgatctctat tcctagttct tcgatcaaag ataaaggtag tgatttgcgt
     2701 cctttttcct tttgggcgtc ccagtaagca atcaaatgaa cactattcaa ataaaaacag
     2761 cgcttcaaag acgaaaacca taacaagaca aagcagaccc cattttgttc gaggcatgct
     2821 ttcatatgtt cgatttgatg ggcatggaag tttttaaagg gaaaagatgt tttgttcttg
     2881 gtttcttttg cttcaaagtc caagtaaaag cctcgataga cgccattata gtctgtagtt
     2941 gaggcttggg taaagtaggc ttccttgatc acagcagcac ttctcttagg atagtctact
     3001 ttcacgattt ggataggggt aggcttttta tggataaccg ccaacccgcg ggaaagataa
     3061 tatttattac tttcattgat tgcttcttca aagcgcatcc cccgattgcc gaattcagtg
     3121 atctgatttc ttttttcttg aattttttcg acgggagagg cattcttata ataaggttgg
     3181 ccatttggat agcgcaatac catctttaat cacactccta ggccacatta tagcaaaaaa
     3241 gcggatagaa agaaaaggaa tctgagatgg ataagtcaaa agtaatctat gtgtctgggt
     3301 atcgaagttt cgaactcggt gtttttaaag aaaatgatcc taaaatccaa gttgttaaaa
     3361 atgttttaaa aaaagaactc catcagttag cagaagaagg gctggaatgg gtacttgtca
     3421 gtgggaattt gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg aaagaagaat
     3481 atccggaact atctcttggt attttgtatc ctttttctga ttttggtaat aactggaatg
     3541 aagggaacca agaaaaaaaa caattagcag aacaattagc agattatgtc gaagctgtta
     3601 gccatcaacc ctaccaatca cctagtcagc taaagaatca cactcgtttt atacttgagc
     3661 atacagtggg ctgtctgctg atctatgatg aagaattccc tggaaaaacg aagtattttt
     3721 ttgaagatgc acagaattac caagcagatc atccctacga aattcggctg atcagtatgg
     3781 acgatcttca aaattttagt gaataatcga tattgtgttt gatttatcaa cttttttcct
     3841 atacaatgaa aaaggtagaa atgttattac gtaaatgaaa actagaggtg taaatatggc
     3901 gaatttggtt tatagtccta aagacatttt acaacaagaa ttcaaaacaa aaatgcgcgg
     3961 ctatgatccc gttgaagttg atgaattctt agacaatatc atcaaagatt atgaaactta
     4021 cagcaaagaa ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa aagtagctca
     4081 attatctaaa actcaaggag cagctcaaac acgcgtgcaa caaacagagg caccaaagag
     4141 cgcagcagtg accaattttg atatcttaaa acgcttgtca aatcttgaac gtgaagtatt
     4201 tggtaaaaag cttgatcaac aagcaagtgc agtcaaacca gcacaaccta atccaaataa
     4261 ctacacaaat gcagacacaa gcttagatga taatgaaaaa acacgtcaat tttaattttt
     4321 gattaatgaa tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc tgaggaaagt
     4381 ccatgctcgc acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc ataagctaag
     4441 gtactctttg gagtaacggc aggaaaacaa gctaaggctt cggctatgct taagtatcct
     4501 tgaaagtgcc acagtgacga agcggctggg gaaacccatc cggtggaacg cggtaaaccc
     4561 ctcgagcgag caacccaaac aatggtaggg gcgctttttg tctggaagaa gaacaggaca
     4621 aaaaggcagt ataactgcag atagatggtt accgctttgt tttttctccc tggaaaagca
     4681 aagtacagaa catggcttat agaaaaccgc aaacatcagg gaattaggtc gtgacaaaag
     4741 taatgcgtta taagaaacaa gaaaaacatt tgaaagctat ttttcaatac ttcagatgaa
     4801 tcgttcttat tacaaaacaa acttttgtat caccggttat tcggaatttg agtaaaagtg
     4861 tggggtagga ctgatgtccc acctcctttt tttataaata aggagattgt gtatggaaaa
     4921 aaacaggaag ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac tggttggtaa
     4981 agaattaaga gatcttggaa tcgaatgcca agttgagaac ggacgtgcac gttttgaagg
     5041 aacaatggaa acgatcgcta cagctaattt atggttacgt acagcagatc gagtaaaaat
     5101 cgtggttggg gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg taaaagcatt
     5161 gccttgggaa gattttcttc cactagatgc agcttttcct gtagctggaa aatcgatcaa
     5221 gtcgaaacta tatagcacac ctgattgcca agcgatcaca aaaaaagcaa tcgttgaacg
     5281 tctccgtacc tattatcacc gtccagcttc tgtaccactg acggaaactg gcgcacattt
     5341 tcagttggaa gtagctttgc tgaaagacca tgtgatggta actctggata cgacaggacc
     5401 aagtttattc aaacgtggtt atcgcttaga aaaaggtgga gcaccattga aagaaaacat
     5461 ggcagcagca cttattatgt tgacaaattg gcgaaaagac cgtccatttt atgatcctgt
     5521 atgtggttca ggtacgcttt gtattgaagc agccttaata ggccataata tcgcaccagg
     5581 attcaatcgt gagtttgctt gcgaatcttg ggattggttc agtcaagaaa tcatggaaaa
     5641 tgttcgatca gcagccgaag aaaaagcaga ttatgatatt gaattagata ttactggttc
     5701 agatatcaat ggacggatga ttgaaattgc caaagccaat gcagaagaaa tcggattagg
     5761 ctcttcgatt acctttaagc aacaagcagt caaagacttc aaaacggata aagaatacgg
     5821 ggtcatcgtg gctaatccgc cttacggaga acgtctagga gaagaagaaa ctgttcggaa
     5881 attatataaa gaaatgggcg atgtattccg tccattaaaa acatggagta aatatatctt
     5941 gactagcgac ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa aaagaaaatt
     6001 gtacaatggt gcattgcgta cggatctatt ccagtattgg ggaactcgtc cgcctagaaa
     6061 aaaagagaaa gacgaataat cttactaatt gattcattca attggatgtt tggaggaata
     6121 gggaatgaaa gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc tacaacaagc
     6181 tttaggaatc ctcgattggg atacacaaac aggtatgcct gaaaaagcta gtgcataccg
     6241 aagtgaagta gacagctatc tttacagcat ttatttttca aaaaaaatcg gtccaaaaat
     6301 caaagaagcc attcgctatt tttctgaaca tccggaagaa ttgtcggaag ttggaactgc
     6361 cgtttttgcc aaagtaaaag aagagtatga tttggaacat cgcgtaccgg aagatcttat
     6421 gagaaaatat acagctgccg tttcttcagc acacggacaa tggcaaaaag ctagagaaac
     6481 acaacaattt tctgattttc aacacgcatt gaatcaaaat attgaattaa caaaacagtt
     6541 gataccttac tggagaaaag atgaaaagac gaattatgat gttttgctga atcaatacga
     6601 acccgggatg actgtcgaaa tccttgataa ggtatttgat cagcttaaag aaggaattct
     6661 ttcgatccgc cgtacgctcc aagaaaaagg gactgaacct gaaacagact ttttaaatcg
     6721 gagaatgacg aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac aacttggtta
     6781 tgattttagt cgtggtcgtt tagatgacac tgtccaccca tttatgactg gaattaatcc
     6841 gaatgatgca cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg ctgtttttgg
     6901 agtgatccac gaggctggtc atggtatgta tgaacaagac atcgatgata aatatgcgta
     6961 tacaccggtt tatgaaggta catcaatggg tatccatgaa tcacaatcgt tgtttaacga
     7021 gatcattatt ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct ttcaagaatg
     7081 tgcagaagga acattcgatg atattccttt tgacaaattt tatcgttcat tgaaccatac
     7141 aaaatcaagt ttggttcgta tcgaggcaga cagtttgact taccctctcc atatcatcat
     7201 tcgttatgaa atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg aactacctga
     7261 aatatggaat caaaaatatg aagagtatct gggaatacga ccaacaaatg atgtggaagg
     7321 catcctacaa gacgttcatt ggtcaggtgc aagctttggt tattttccct cttatgcatt
     7381 agggtacatg tatgcggcac aattacttca ttctttgcag caagaaatag atgtggaaaa
     7441 tgttctagcg tcagacgatt attctccaat caaaaactgg atgtcagagc atatccatca
     7501 atatggtgca tctagaaaac caaatcaatt aatcatggat gcaacacatg aaccgcttaa
     7561 tccgcaatac ctgatcgact atatgaaaga cctgtatttt aatgtatatc aggtaaaata
     7621 aaatacaatt tacaaagata gctgaaattc atctaatcta tagatgtttt cagctattta
     7681 cttaagaaag aagtggaacg aatggataaa ttacaagtga tcgctgatta tagtatggac
     7741 caattagcgc atgatcaaac aggacatggg agcgatcata ccaaaagagt tgtcaagtta
     7801 gctgaaaaaa ttttggagac ggaaccgcag gcagaccgct ttgtgacgct tgcagcagct
     7861 tacctacatg atacgattga tgataaagtc gttaaaaatg agaacgaggc aaagcaacag
     7921 cttcgtgtat tccttcgctc tttgccaata gtagaggaac agatctcgat gattttttcc
     7981 attatcgaaa atatgtcctt ttctaaaaat ctctctgaag cggccgaact atctctggaa
     8041 ggtaagatcg ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg cattttgcgt
     8101 actgcttatt tcggcggtgg acatgggcac ccgatttttg atcctgaact gtatccgcaa
     8161 acattcaagg acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca tttttatgaa
     8221 aaactgttct tgctgtctga taaaatgaat actgattacg gatatgaaga agccaaacga
     8281 agagaaacct tcatgcgtga attcttagaa gaattttttc tggaatggtc tattgacgat
     8341 gaaaatttta ctccagacaa ttggatcaaa aataacaaaa aatgatggag gttgtgacag
     8401 aagtggtcag ctccaagata tttcggatca ttttccgtaa aagttacttc tggaacactg
     8461 tttaccaaga ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca atttcaaaaa
     8521 atagcttctc gtgtttgatg aatattgtaa cttgtgattc ggaattggca ggagaacacc
     8581 gtttatt
//
LOCUS       DASNNU010000102         8494 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.102, whole genome shotgun sequence.
ACCESSION   DASNNU010000102 DASNNU010000000
VERSION     DASNNU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.102"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..173)
                     /locus_tag="U8116_002349"
     CDS             complement(<1..173)
                     /locus_tag="U8116_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8435304.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="U8116_002350"
     CDS             complement(593..1714)
                     /locus_tag="U8116_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEN8435305.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="U8116_002351"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="U8116_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEN8435306.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="U8116_002352"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="U8116_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEN8435307.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="U8116_002353"
     CDS             complement(4304..5215)
                     /locus_tag="U8116_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN8435308.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="U8116_002354"
     CDS             complement(5232..6239)
                     /locus_tag="U8116_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN8435309.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="U8116_002355"
     CDS             complement(6236..8365)
                     /locus_tag="U8116_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN8435310.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="U8116_002356"
     CDS             complement(8370..>8494)
                     /locus_tag="U8116_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN8435311.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DASNNU010000103         8455 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.103, whole genome shotgun sequence.
ACCESSION   DASNNU010000103 DASNNU010000000
VERSION     DASNNU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.103"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            245..514
                     /locus_tag="U8116_002357"
     CDS             245..514
                     /locus_tag="U8116_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005231967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8435312.1"
                     /translation="MDTKEFDQIKKIISKKIKEERIKHGYSVRTLADLTNIGFSHISR
                     LENNKIPSPRLETYLIIFEVLEISLGEVFSDLDNLPPFNRYPPDH"
     gene            complement(581..895)
                     /locus_tag="U8116_002358"
     CDS             complement(581..895)
                     /locus_tag="U8116_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435313.1"
                     /translation="MTEEEVAALNRIKEWSVLLKIYYGIMILSFITAGLFSFLLVLLS
                     LWTNLMLFLYLLLLFFLGVGGVMTAILDDIKWSLFSGVCKKMKRTATIEFYECWRNDV
                     GL"
     gene            complement(899..1186)
                     /locus_tag="U8116_002359"
     CDS             complement(899..1186)
                     /locus_tag="U8116_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEN8435314.1"
                     /translation="MSINTFVYPYPINVFIISRLGMTVEQFCELHGYKQSTVASWVTR
                     NRAVKTLPCDFLYCLSLSSGWSMDDVYRHLLVLEERFLKSMDPKKKKEQVD"
     gene            complement(1322..2821)
                     /locus_tag="U8116_002360"
     CDS             complement(1322..2821)
                     /locus_tag="U8116_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN8435315.1"
                     /translation="MAASLDVVYSTVLQNGIRKFKYKNSHLKPVSFSDQSGKGAIFAY
                     RSKEHMIEGIGLVITSEEGVIENNNRFTHWTPNVFRYGTYADEARMFTKGHSEDNLRQ
                     INTFFVDFDTLDPNFDYGEIILASHEIGFMPTMILRTPHGFQAFYVLDKPAYVTKKSN
                     FKVINVAKMISKNLRNKFSNDYRFPVDVNANHFGITRMPSEENVLFFEPQYRYSFEEW
                     IDWSMREDADKKAEIKNVFVLPSTQEFRQVDEPWFQLLLKSKKIQGEKGVYGRNNVIF
                     TLSLAYFSSGYEVEACELTMLEFNDRLANPLSDRELTRIIRSAYSGKYQAAQREKIVL
                     LCQEWVDANLSEEQLFIKKRGWWKFKKERAVRKYSHSHEWAEDLLSYLNEQSYEEQPY
                     IISTKKELTESLKIPKRSLDKVLKQLREENKIHFLVRAGRNGGIMLASIHGLMRSVIR
                     LKKESQGAYFAAISEAFGREYTFIQTALKRLIEPNTIGVQTDLFMIDTG"
     gene            3479..4336
                     /locus_tag="U8116_002361"
     CDS             3479..4336
                     /locus_tag="U8116_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN8435316.1"
                     /translation="MKKNRSNSAEIISLVKQVKDLVAEKGGPIVISVANQKGGVGKSA
                     TVDNLAELFASFDLITDIVDIDAQSSITNLKTDLRNIIDENLPEMTQVMLEEETLENI
                     TYQIKNNLFISPTTLRLSDAELNLVNATLRELILKKNIESLETKFDIILIDCPPSRGL
                     LTVNALSASDYILIPVQSEYQALVSIELLFSTINKVKNSINPNLKELGYVVTMATNTN
                     HSDEIIEEVKSDDKAEVISIIDRSIVVSDAGVANMSAYEFDSNNKAGIAYYNLAVTVL
                     QKIVSEGVV"
     gene            4338..4694
                     /locus_tag="U8116_002362"
     CDS             4338..4694
                     /locus_tag="U8116_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435317.1"
                     /translation="MAKRVSIKDKNIAKTNDIDTLFPTSKPQTSDQNGTKKKINILRK
                     IEDIISYYKNFKGGKQVSIYLPEEIHRMVKMKAAQDDRNMKDVMKDLILDNYLTESEI
                     KEAYRSYVDKLEEDSK"
     gene            5179..6558
                     /locus_tag="U8116_002363"
     CDS             5179..6558
                     /locus_tag="U8116_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN8435318.1"
                     /translation="MVMQFGVNPRFDYTKEPSRDILCIDCKSFYSSVECVERGLNPLK
                     TKLVVMSYPSDDPSQRGSGLILASSPAAKKVYGISNVSRSRDLPFPYPEDLVIAPPRM
                     ALYMRKNMEINNIYKKYADEQNHSVYSIDESFVDVTDSLKLFGAKDARELARMIQTDV
                     YRQTGIFTTIGIGDNPLLAKFALDLESKKNSDMKAEWRYEDVQQKLWSVENITDVWGI
                     GRRTAIRLNRMGIFTMHDLAHANYYQLKQNFGVLGTQLYAHSWGIDRSFLGQKYKVKS
                     KSIGNSQVLNRDYTRRKEIEIVIKEMADQVATRLRRTGAKAEVVSLWIGFSMGYVDQS
                     GSRGFHQQMKVPATNSSKQIANYLLQIFDRHYKYQDIRNVGVNCSKLVYSNALQLDLF
                     EDPDEQVKDLKIDYVVDTIRKKFGFKSIVHANSIMEGGRAIARSSLVGGHAGGMSGLE
                     GAESHGKAY"
     gene            6542..7048
                     /locus_tag="U8116_002364"
     CDS             6542..7048
                     /locus_tag="U8116_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005231970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435319.1"
                     /translation="MVKRTKKTFTDYNQYRDRPFGLKWATAYAMDDLMKGVLENEAWA
                     TKEVVGLELMNREEIDTILSESFLYHKPITLQLNTKDEFGRFLDPVEGQFLGEAYEDY
                     FVLNEQLIYWTDVRHISIKKEEKWFKVDYFQDGKSSRNKQKKKASKIEKEIELIKNEY
                     YQDFADDD"
     gene            7140..7730
                     /locus_tag="U8116_002365"
     CDS             7140..7730
                     /locus_tag="U8116_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEN8435320.1"
                     /translation="MCGRYFFDLSSNELRSYYDHVVLNATGKHIRVGFNEIFPTNYIV
                     TLGLNQDSRVVPGITQWGFQGFKPGQLMINARSETVEEKKTFSKAFRETRCVIPVSGF
                     YEWDSEKRKLLFTAGDNDVFYLGGFYRVHKTGAGFETESIIMTTKPNESVSPIHDRMP
                     LIIQRDHIKDWITDLDFARNYLTADMPELTRTEVAK"
     gene            complement(7746..7967)
                     /locus_tag="U8116_002366"
     CDS             complement(7746..7967)
                     /locus_tag="U8116_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435321.1"
                     /translation="MDEEKIFDKRWQLASTEQRARFNNLLSSYPTIDWTFKEKKYLLW
                     LSQLDIDTFETFEVILDKIKHSNEKRANL"
     gene            complement(8022..8360)
                     /locus_tag="U8116_002367"
     CDS             complement(8022..8360)
                     /locus_tag="U8116_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435322.1"
                     /translation="MKNKEILNRLLYLTADEEWMNRPDIVASVQKIGKEIACKERSRR
                     NFSNTKGVKVTDYADNVRLYSSVTECMKHEKLCRSSITGHIKRKSQAKDGRRFFVIED
                     DNLLFQNDDV"
BASE COUNT         2840 a         1534 c         1454 g         2627 t
ORIGIN      
        1 attgttttaa aatttcatgt gtaactttat cacagtaaat ataattttac aacaatacat
       61 tattaggtta agaggacaaa aacttaacca aataactata tcggcataaa aataataaat
      121 ttaaggttat ttctaacaat cttttcaatc ttcactatta caataaaatt atacagctag
      181 ttgtcttatt ttgcaacgat aattcgtaag caatggtatt ttttatctaa aaggattgct
      241 agacatggac actaaagaat ttgatcaaat caaaaagatt attagtaaaa aaataaaaga
      301 agaacgtatt aagcacggct attccgttcg aactttagct gatttgacta atattggatt
      361 cagtcatata tcaagattag aaaacaataa aatacctagt ccaagattag aaacttatct
      421 aataattttt gaagttttag aaatctcatt aggcgaagta ttttctgatc tagacaattt
      481 accccctttt aacagatacc ccccagatca ttaaaaaatt gtgaaaagaa atttaattct
      541 tttcacaatt ttttttactt gataaactta ctttttatat ctacaacccc acatcattcc
      601 tccaacattc gtaaaattct atagtcgcag ttcttttcat tttcttgcaa actccactaa
      661 acaaagacca cttaatatca tccaaaatag cagtcatcac tcctcctacc cctaaaaaaa
      721 acagtaaaag taaatataaa aataacatca gattcgtcca caatgacaaa agaacaagca
      781 aaaaagaaaa taatccggcc gtaataaaag aaagaatcat aataccataa tagattttca
      841 aaagaacaga ccattctttg atcctattaa gagcagctac ctcctcttcc gtcatcaatc
      901 aatccacctg ctccttcttc ttttttggat ccatactctt taaaaatctc tcctccaaca
      961 ccaataaatg acgataaaca tcatccatcg accagcctga agaaagacta agacaatata
     1021 aaaaatcaca aggtaatgtt tttactgcac ggtttcttgt tacccaactt gctactgtac
     1081 tctgcttata tccatgcaac tcacaaaatt gctctactgt catacctaac cgcgaaataa
     1141 taaatacatt gatcgggtag ggatatacaa aggtattgat actcatagtc agaaagacct
     1201 cccaccatat tatcacgttt gcgataatag tactattctt tcttaagacc agcaagcaat
     1261 gcggagaaaa aaaggaacgt atcatagata taatgtaata ggcaatttgc accttttaat
     1321 ttcaacctgt atcgatcatg aataaatctg tttgaacacc aatcgtattg ggttctatca
     1381 agcgtttcag tgctgtctgg ataaatgtgt attctcttcc aaaagcttct gagatcgcag
     1441 cgaaataagc cccctggctt tcttttttta gccggataac ggagcgcatc aatccatgta
     1501 tcgaagctag cataatacct ccattacgac cagcacgaac aagaaaatga attttgttct
     1561 cttctcttaa ctgcttcagt actttgtcca gcgaacgttt tgggatcttc aaactttcag
     1621 ttaactcttt ttttgtagaa ataatgtatg gttgttcttc ataggattgc tcattcaaat
     1681 aggacagtag atcttctgcc cattcatgac tatgtgaata cttgcgaaca gctctttctt
     1741 tcttaaattt ccaccaaccc cgttttttga tgaatagctg ctcttcagaa agattggcat
     1801 cgacccactc ttggcataat aggacaattt tttctctttg cgctgcttga tattttccgg
     1861 agtaagcgga acgaatgatt ctggttaatt ctcgatcaga taaagggtta gccaagcggt
     1921 cattgaattc taacatcgtc aattcacatg cttctacttc gtatcctgac gaaaagtatg
     1981 ccaaagacaa ggtgaatatg acattatttc gaccatatac gcccttttca ccttgtattt
     2041 ttttactttt tagcaacagc tggaaccagg gttcatcgac ttgacgaaat tcttgagtag
     2101 atggtaatac aaaaacgttc ttgatctccg cttttttgtc cgcatcttct ctcattgacc
     2161 agtcgatcca ttcttcgaaa ctataacgat actgtggttc aaagaataaa acattctctt
     2221 cagaaggcat tcttgtgatt ccaaaatgat tggcatttac atcaacagga aaccgataat
     2281 catttgaaaa tttgtttctt agattttttg agatcatttt ggccacattg atcactttaa
     2341 aatttgattt cttcgtcacg tatgcaggct tgtctagtac atagaaagct tggaatccat
     2401 gcggtgtacg tagaatcatt gtcggcataa agccgatctc gtgactggcc aagataattt
     2461 ctccatagtc aaagtttgga tccaacgtgt caaaatcaac aaagaatgta ttgatttgac
     2521 gtaaattgtc ctccgaatga ccttttgtaa acattctggc ttcatcagca tacgttccat
     2581 agcgaaatac attaggtgtc caatgagtga atcgattatt gttttcgatc accccttctt
     2641 ctgaagtgat cactagtcca atcccctcga tcatgtgttc ctttgaacgg tacgcaaaaa
     2701 tagccccctt ccccgactga tcactaaaag agaccggttt taggtggctg tttttatatt
     2761 tgaatttacg gataccattt tgtaataccg tcgaatatac gacatccaaa gaagctgcca
     2821 tgactttatt ccttcccaaa ctaaaagaga acacaaataa tcttaaagat ttgtgttctc
     2881 ccaatagctt agaagtctta aatgaatgac aaaataagcg tgtatatatt gagttgctcg
     2941 tcaaaaaatg gtagaataag tctgtaagtt cggattgtca gtccgaaaga cttatcaaaa
     3001 ttacataagt ggttgtcagc cacttacata acttctgaga cgagcgagaa cacatagttg
     3061 ttgtatgtga ttcagatgaa agagtgaccc aattcacttt tttatccgtc tcttttttta
     3121 tttttatttt tttcagttct ataaatacaa acatagcata tcttactttt tccatcttgt
     3181 aaactcttat tttttataat tttatatagc tattatcgca caaacctatt aataccaaca
     3241 attacattag gcattaaaac acttaagtac ttaagtgttt taatgccttt tttcagtact
     3301 taagtactat gacgttttga agttgtattt aattgctgat aaatcacatt ctaaacatag
     3361 tcgaattaaa agtacttaaa tactaaagtt gctattgact cataactttc taggtggtat
     3421 tattatattg catgaaagta ttttaacact ttagtactta actcaaggag gtaggaaaat
     3481 gaaaaaaaat cgatcgaatt cagcagaaat tatttcgcta gttaaacaag taaaagatct
     3541 cgttgctgaa aaaggtggtc ccattgtaat ttctgttgca aatcaaaaag gtggcgttgg
     3601 aaaaagtgct accgtagata atctagctga actgtttgct agtttcgatc ttataacaga
     3661 tatcgtagac atagacgcac agtcaagtat aactaatcta aaaactgatc ttagaaatat
     3721 catcgatgaa aatctaccgg aaatgactca agtaatgcta gaggaagaaa cgttagagaa
     3781 cattacttat cagattaaga ataatctatt tatttcccca acaaccttgc gtctatcaga
     3841 tgccgaactt aacctagtta acgctacatt aagagaactg attctgaaga agaacataga
     3901 gagccttgag acaaaatttg atatcatttt gattgattgc cctccttcaa gaggactgct
     3961 tactgtaaat gctcttagtg catcagatta catcttaatt cctgttcagt ccgagtacca
     4021 agctctagta agtatagagt tgcttttttc tacaatcaat aaagtgaaaa attcaattaa
     4081 tcctaatcta aaggaacttg gctatgtagt cactatggca actaatacaa accacagtga
     4141 cgaaattatt gaggaagtta aatctgacga taaagcggaa gttatttcta tcatcgatag
     4201 aagcatcgta gtatccgacg ctggcgttgc taacatgtca gcttacgaat ttgattcaaa
     4261 taataaagct ggcattgcat actataatct tgcagtgaca gttctacaga aaatagtatc
     4321 agaaggagtt gtataaaatg gctaaacgtg tatctattaa agacaaaaat atagctaaaa
     4381 caaatgatat cgacacacta tttcctactt caaaaccaca aacttccgat caaaatggaa
     4441 ctaaaaagaa aattaatatt cttagaaaaa ttgaagatat aatttcatat tacaaaaatt
     4501 ttaaaggcgg taaacaagtt tcgatatact tgcctgaaga gattcatcga atggttaaaa
     4561 tgaaagccgc acaagacgat cgaaatatga aagacgtaat gaaagattta attctcgata
     4621 actatctaac tgagtcagaa attaaagaag cctaccgttc atacgttgat aaattagaag
     4681 aagatagcaa atagacatga gacactttag tactaaaaca ttttttttgt ttagtgctaa
     4741 agtgtctttt tcatgcatca tcatcacacg aactttttag tactaaagta cttaacaatt
     4801 taagtgctta accattaaaa cgttttgcta ctgatttctt tcgacgaagg caatcgaaac
     4861 ttcgtaatct ttattttttt taataatttt agggggactc cgcagtttct tattgtattc
     4921 agagtcttta atatttaagt atttaagtac tttataacta aaacacttaa gacctttgga
     4981 cttacggagt gtattgtttt aattatccaa acggtgggac gttcacctat ttaaacactt
     5041 tgacactttg gtattttgac acttaaccat taaaaccgca aagatagaat tttgatctgt
     5101 tctcttgact tagttagcga acgtatgttc taatacggta tataaaatgt atcttttttt
     5161 tagaaaaggg gatttatcat ggttatgcaa tttggcgtaa atccaaggtt tgactatact
     5221 aaagaaccct cacgagatat tctttgtatc gactgtaagt cattttattc atccgtagaa
     5281 tgcgtagaaa gaggactaaa tcctttaaag acaaagttag tggtcatgtc ctatccttca
     5341 gatgatcctt ctcagcgagg aagtgggttg atcctggcat cgagtccggc cgcaaaaaaa
     5401 gtctatggaa tttcaaatgt tagtagatca cgagatcttc cttttccata tccggaagac
     5461 ctcgtgattg caccaccgag gatggcactg tatatgagaa agaacatgga gatcaacaat
     5521 atttacaaga agtatgctga tgagcaaaat cattccgtat acagcatcga tgagagcttc
     5581 gtggacgtca cagactcatt aaagctgttc ggtgcaaagg acgcaagaga gctggcgaga
     5641 atgattcaaa cagacgttta tcgacaaact ggaatcttca caacgatcgg gataggtgac
     5701 aatccgctct tggccaagtt cgcactagac ctcgaaagca aaaaaaattc agatatgaaa
     5761 gcagaatggc gctatgaaga cgttcagcaa aagctttggt ctgtagaaaa cattacagac
     5821 gtttggggga ttggacgtcg tacagcaatt agactgaaca gaatgggcat atttactatg
     5881 catgatctgg cacacgcaaa ttattatcag ctgaagcaaa attttggcgt cttaggtact
     5941 caactgtatg cacacagctg gggcatcgat cgatcattct taggtcagaa atacaaagta
     6001 aaatctaagt cgatagggaa cagtcaggtc ttaaatcggg actatacacg ccgaaaagaa
     6061 atagaaattg tcatcaaaga aatggccgat caagtagcaa ctaggttacg ccgtacagga
     6121 gcgaaagctg aagtcgtttc tttatggatc ggcttttcaa tgggctacgt cgatcaatca
     6181 ggaagtcgag gatttcacca gcaaatgaag gtgccagcaa ctaatagcag caagcaaata
     6241 gcaaactact tactacagat atttgatcgt cattacaaat accaggacat tcgaaatgtg
     6301 ggagtaaact gttcaaagct cgtttattct aatgctttgc agcttgacct ctttgaagat
     6361 ccggacgagc aagtaaaaga cttaaaaatc gattatgtgg tagatactat acgcaaaaaa
     6421 ttcgggttta aatcgatcgt tcatgcaaat agcataatgg aaggtggacg tgcgatcgct
     6481 cgcagcagtc ttgtcggagg acatgcaggt ggtatgtctg gacttgaagg tgctgaaagt
     6541 catggtaaag cgtactaaga aaacatttac ggactataat caatacagag accggccatt
     6601 tggattgaag tgggcaacgg cttacgccat ggatgatttg atgaaaggcg ttttagaaaa
     6661 tgaagcctgg gctacgaaag aagttgttgg tttggagtta atgaatcgtg aggagatcga
     6721 cacgatactt tcagagtctt tcctttatca taaacctata acgctgcaat taaacactaa
     6781 agacgaattt gggcggtttc tggatcctgt tgaaggacaa tttctaggtg aagcatacga
     6841 agactatttt gtattgaatg aacaactgat ctattggaca gacgttcgac acatatctat
     6901 aaaaaaagag gaaaaatggt tcaaagttga ctattttcag gacggaaaat cttctcgcaa
     6961 caaacaaaag aaaaaagcga gtaaaataga aaaagagatt gagttaatca aaaatgagta
     7021 ttatcaggac tttgcagatg atgattgaag atccttatcc atcatttgac tttgaaatcg
     7081 ccttagaata gctctctgac acacgttttt taaacaaatc agaaaaggag ggggaaaata
     7141 tgtgtggaag atattttttt gatttatctt caaatgaatt aaggagctac tatgatcacg
     7201 tcgttttaaa tgctacaggt aaacatattc gtgttggttt taatgaaatt tttccaacta
     7261 attatatagt aacactagga cttaatcagg attcaagggt agtaccagga atcactcagt
     7321 ggggattcca aggatttaag cctggtcaac tgatgataaa cgcacgctca gaaacagtag
     7381 aagaaaagaa gactttttct aaagccttca gagaaactcg ctgcgtcata ccagtttctg
     7441 gattctatga gtgggattcc gaaaaacgaa aactattgtt tactgcaggg gataatgacg
     7501 ttttttattt gggaggattt tatcgtgtgc ataaaacagg tgcgggtttt gaaacggaat
     7561 cgatcattat gacgacaaaa cctaacgagt cagtatcccc aatacatgat cgtatgccgc
     7621 ttattattca aagagatcat ataaaagatt ggatcacaga tttggatttc gcacgtaact
     7681 atcttacagc tgatatgcca gaactaactc gtactgaagt agcaaaataa aaaagagcgg
     7741 atcttttata gattcgctct tttttcattg ctatgtttaa ttttatctag gatcacttca
     7801 aatgtctcaa atgtatcgat atctaattga gacaaccata gtaagtactt cttttcctta
     7861 aatgtccagt cgatagtagg gtaggaggat agcaaattgt taaatcttgc tctttgttcc
     7921 gttgaagcta attgccagcg tttgtcaaaa attttttctt cgtccacgta aaaacctcct
     7981 agctaaagta cgattatctc aatttgaaac ttattattga ctcacacatc atcattttga
     8041 aaaagcaaat tgtcatcttc tattacaaaa aatcttcttc catctttggc ttgagatttt
     8101 ctttttatgt gaccagtaat agaagatctg cataactttt catgcttcat acattcagtt
     8161 acagacgaat aaagtcggac attatctgca taatcagtta cttttacgcc ttttgtgtta
     8221 gaaaaattgc gacgagaacg ctctttacaa gcaatttcct tcccaatttt ttggacactt
     8281 gctacaatat ctggccgatt catccattct tcgtcagcag tcaagtacaa caatctattg
     8341 agtatttctt tatttttcaa attaatttac ccccgccaaa actatcaaga atactatatt
     8401 ttacttaatt ataaaaactt aaattttctg tatccttttc aaaaaataag cttag
//
LOCUS       DASNNU010000104         8372 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.104, whole genome shotgun sequence.
ACCESSION   DASNNU010000104 DASNNU010000000
VERSION     DASNNU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.104"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(64..1032)
                     /locus_tag="U8116_002368"
     CDS             complement(64..1032)
                     /locus_tag="U8116_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEN8435323.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1264..2097
                     /locus_tag="U8116_002369"
     CDS             1264..2097
                     /locus_tag="U8116_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEN8435324.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2131..2970
                     /locus_tag="U8116_002370"
     CDS             2131..2970
                     /locus_tag="U8116_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN8435325.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            2996..3556
                     /locus_tag="U8116_002371"
     CDS             2996..3556
                     /locus_tag="U8116_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN8435326.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3755..5476
                     /locus_tag="U8116_002372"
     CDS             3755..5476
                     /locus_tag="U8116_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEN8435327.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5641..6783
                     /locus_tag="U8116_002373"
     CDS             5641..6783
                     /locus_tag="U8116_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN8435328.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6799..8010
                     /gene="thiI"
                     /locus_tag="U8116_002374"
     CDS             6799..8010
                     /gene="thiI"
                     /locus_tag="U8116_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEN8435329.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2873 a         1450 c         1739 g         2310 t
ORIGIN      
        1 ttttcgaaga agctattttt gtcacagcct ctctttttaa gtttagctat tagataatga
       61 tgtttagttt tggataaacg aaatcaattc ttcacccgac aaaccatagt ttacagcaat
      121 caatagtttt aaacgtgctt tttgactatt aatactgtta cagaaaataa tccccatttt
      181 ttctagttct tttcccccgc cttcataatc ataaacaggc tctgcgattc cattgaaaca
      241 acgagaaaca agtactacag ggattttttg gacaagaagc ttttccaaag cagctaaggc
      301 ttgtggaggg agattccccg cacctaaagc ttcgattacc aaaccgtcta ctttcgtatg
      361 ggcgatggct tccaataagt ccccttgcat cccagcatat gctttaacga tcgggatagt
      421 cccatctaca gcggaaatgt ccagtcgctt tgtttctaaa agttcctgca agaataaaat
      481 tcgattctta gttactaagc cgatgggacc caatgtaggt gttcggaaag tagcaacatt
      541 tgtggtatgg gtctttgtca cgtagcgtgc agaatggatc tcatcgttca tgaccaccaa
      601 aaccccctta tctagcgctt cttcacaact tgcgacacga atcgcacttt caaaattata
      661 aaggccgtca cttccaagtt cattacttga acgcattgcc ccagtcaaaa caattggtaa
      721 tttgttgcca atcgttgtat caagaaaaaa agcagtttct tctaatgtat ctgtaccgtg
      781 tgtgatcacc actccggata tacctgatgc ataagcggac aagatacgtt cttttaatac
      841 caacatgtga gaaggcatga tgtgtggtga gggaagattg aagatatctt caactaataa
      901 ttctacatgc ttggggattg tgatttctgc atctaataat gggtttgcca catcaggact
      961 aacaccgccc gtgagcatgt tttctttcat ggcgatcgtt ccgccggtgt gtagtacaag
     1021 tatttttttc attttacaga cttccttacg aataattttg catagccgtt aaatcttatt
     1081 gtatactata taaaagagtt ttgctatcag tgaagaataa cgaatagcct aacttatcat
     1141 aaaaaagctg accttattaa aatggaaaag actcaacctt gacctcctaa tacaatggag
     1201 tatctggttg agtttctttt acaagaaagc agccaaggaa atcaatcacg aaggtggccg
     1261 gaaatgatca gaatcggatt aacatcgttt aaagaacatg acaaattaac tggtaaaaag
     1321 aataacactc tttatgaata tgcagggttt ctacccttag tggaattaga tactgcctat
     1381 tatggaattc cccgaaagtc ctcagttgag aattggataa gtgaagtacc tgaatcattt
     1441 cgctttgtag tcaaagtcta tcaaggaatc agcggtcaag gagactggaa gccttattat
     1501 cagaccgaag aagagatgat tgcgcatttt ttagatgtta tgtcgccaat ggtagatgct
     1561 ggaaagatgt tttgcttttt agtccaattc ccctcatcat tcaaatgtac gaaagaaaat
     1621 gtccagtact tgcgcaaact tagaaagtgg tttgagggtt atcctgtttc tatagaattg
     1681 cgggattatt cttggtacag cgatgaattt cttgaacgta caagaaactt tatgtctaga
     1741 gccgcttttt ctttagccat catcgatgag ccacagttgc tgaatacaac gattcctttt
     1801 gatccatttg tcaccaatcc gaactttgct ctttttcgat tccatggtag gaatgcgcaa
     1861 ggatggcaag caaatgataa agagtggcgt aaaaaaagaa cgttatatcg ttattcagaa
     1921 aaagaactgc aagaactagc acatgaagtc aaagtagttg attctaagac aaaagaggta
     1981 ggtgtgattt ttaataataa ttctggtggt gatgcggctg aaaatgcatt agcactgcaa
     2041 aaaatattaa atatcaagcc agatggactc aatccaaccc aaatggatct gttttaaggg
     2101 cttattttat tcaagaaagg ggcgaagtct atgatcaaag ctattttttt tgatattgac
     2161 ggaacattgg tcaccaatcg ttcaaaagcg ttggaatcaa caaaacaagc aattgaatat
     2221 gctcggaaaa acggcatttt atgcggtgta gcaacggggc gcagcccagt aaagatcaaa
     2281 gaaatcattg atgaattgga attagatatg tatgtcgtgt ataatggtca actggttttt
     2341 actgcagatc gcacgattat cgaccaccct tttgaacaaa aagtattgga acatattgtt
     2401 gaatttgcag atgagaacca tcgacaaatc gtttttggtg cgagaaatcg gttagatgga
     2461 tcaacgacga tgctattagg acaatcgatt tttatcaagc gactagtcag ctttttaccg
     2521 cgtaaattcc cggtacgtct gatgaaaaaa atcctccagg tttttagccc tcaccgacaa
     2581 aaggatcgtt atgaaacgtt agagatatta aaagaaccaa tttatcaatg tatattgctc
     2641 agtccggagt cagaacagaa aaagttagaa catcgctttc ccgagtgtac gtttcagcgt
     2701 tcaaatacgt atactgtaga tatcatccca aaaggtggtt caaaattact aggcatccaa
     2761 gctttcgcaa atgctgtcga aattgaaatg gaagaaatta tggcattcgg cgatcattac
     2821 aatgatatcg agatgctgaa aggtgtagga attggagtgg ccatggggaa tgctcagatc
     2881 gaagtaaaac atgctgcaga ctttgtgact caatcaaatg aagaagatgg gatatacgaa
     2941 ggactgaaac acttcggtct gatttattag tcaatgaaag aaggaaaaaa tcagtatgga
     3001 agcagagaca ccttttcaaa aagtagaaac attccacaaa acttttgatc caagaagacc
     3061 agagataccc acggctttta cagaaaatga agctcataca cgtgctgcgt tcaaaataga
     3121 agaacttgtt gaatttgttg cagctacttc acctaataaa gaacaactgc atgaagctgt
     3181 tatgctgctg cacgaagcat tagataaagc agaagaaaaa atacttggta aaacacagtg
     3241 gggaagtgat ccgctggttg aacaagtaga tgcactgaca gatatccttt actttactta
     3301 tggttctttt tcattactag gaatagtgcc tgatcgaata ttcgaaatcg tccatcaggc
     3361 aaatatgggg aaattatttc cagatggaaa acctcattat gaccctgtaa caaataaagt
     3421 gttgaaacct gaaaactggc agatggaata tgctccagaa cctaaaatcg cggcagaaat
     3481 ccaacgtcag attcaagaag cgattgctaa gaagaaatcc gctgatatag gcatgtgcac
     3541 aaatgaaaaa gagtgatcag agtttcacta tttttgttat ctttatacga tggaaaaaca
     3601 aaagattttc gtttaagtta gtgaatgcat gaaaatagta tgctataatc tagaatagat
     3661 gtcttttgca cgagagacgt attatttgga tttatgccaa gcagaacgcc atctgttgca
     3721 taaaagatgc atactgaaaa cggaggaacc ataaatggga aataatgtta tcatcggaat
     3781 cgtcattgca atcattatag tcgccattgt cgtctttttg gttagttact tgatgaagaa
     3841 aaaaaaccaa gatcgattgg atgccttaga aaaacgcaag gaagaattat ttgacttgcc
     3901 agtgattgaa gaagtcgatg atgttaaaaa aatgcatctg gttgggcaaa gccaaaatac
     3961 ctttcgtgaa tggagtcaaa aatggaatga catatctact tcctcgttcg ctgaactaga
     4021 aagtcaaatt ttcgaagtgg aaaacctcaa tgaaagtttt cggttcttaa aaggaaaaaa
     4081 agcagttgtt gaagcagaag ctacaatgga ccaaatggag aaacaagtga cggaaatccg
     4141 tgaaggtcta aaggaattgc gggaaacgga agaacgcaac tcactagctg ttcagcaggc
     4201 acttgacgtc tatgaagaac tgaaagaaaa agtcagagaa gacgagcatg aatacggacc
     4261 agctcttttt gaattgcaaa aacagatcaa agtgatcgaa aaagaattta cgcagtttgt
     4321 gactttaaac acttctggag accctgttga agcccgcgag gttttggata aagcagaaaa
     4381 acatacgtat gaagtggaag ctctgatgaa aaaaattcca ccgctgtatg aggagctcca
     4441 taccactttc cctgaacaac ttgaagaaat cagtgatact tacgagaaat tgatggaaga
     4501 acaatatgta ttcccagaag aagatcttgc tgaagatatc gcaaaagtaa gtcgaagaat
     4561 tgaaaattca ttagccaatc ttgaaaaaac agaagttgaa acggtcgaat ttgaaaaccg
     4621 tgaaacagct gatttgattg attcgttata tgatatctta gaacgggaaa tggaagctca
     4681 gagatatgta aaaactaacc aatcaacgat tgcagagtat atcaaacata caacaaagaa
     4741 taaccgtcaa ttattgattg agctggatca cacatctcaa agctatacat tgaaccacaa
     4801 tgaaattggc cgtgtacgag gtttccaaac cgaagtggaa gaaatggaac gtcaaaatga
     4861 acagatgatt ccacagatcc gtcaacacga aattccttat tcagaagtgc gcacgttcta
     4921 taaaacagtc ttcaaagtct tagaagatat cgaaaagcaa caagtagaga tcgacgactc
     4981 attacatgaa ttgagaaaag gcgaaaaaga agcgcaagaa aagatcgata cattcgaatt
     5041 caaattgcgc agtttgaaac gttttgtaga aaaacaacgt ttgcctggct tgccgaatga
     5101 ttacctagaa ttcttttttg ttgctactga ccgtattgaa gaactaagtg ttgtcttgaa
     5161 taaaattcga gtaaacatgg aagaggtcaa tcgtttagtt gctctatgtg aagaagatct
     5221 agaattatta gataagaaga cacatgattt agtagatgca gcagcattaa cggagcaaat
     5281 gctccaatat gcaaatcgtt atcgtcatac acacgaagaa gtgagagaag caattgataa
     5341 aagctactat ttattcaata aagaatatca ctatcaagaa gcattagacg aaatcggtac
     5401 agcattagag cgagtagaac ctggtgcttt caaacgcatc gaagattttt attttaatca
     5461 tccagactta gtataaagca aaaaagttag taaaccagca agagcaagac ggattattgt
     5521 cttgttcttg tctttttttg tatatttgaa aattgaaaca agtgaaaatt tcacctgctc
     5581 ttgaaagcac cttggacaac cgttataata gaaaagaatt tcaaaaaaga ggggtttctt
     5641 atgatatatt ttgataacag tgcaacaacc ccaatctttc cgcaaagctt agatgcatat
     5701 gtaaaaacca gtcagcggat tattgggaat ccatcaagct tgcatgattt aggcaatcaa
     5761 gctaaccggt tgttgcagca agcaagaaag cagatcgctg atttgatcca tgtagaacca
     5821 gaagaaatct attttacttc aggcggaaca gaaggagata actgggtatt aaaaggaacg
     5881 atgatcgaaa aaagagaata tgggaatcat atgatcattt caggagtcga acatccggcc
     5941 gtcagcgaga cagcagaaca attaaaagaa ttaggaatcg aagtaagtat cgccccagtg
     6001 gataaacgcg gatttgtacg tgtcgacgaa ttgaaagaat taataagaaa agagacagta
     6061 ttagtctctg ttatggctgt gaataatgaa gtaggatcga ttcagcccat ccaggaaatc
     6121 agtgatcttt tggatgattt tcctaaaatt catttccatg tagatgctgt tcaggcgatt
     6181 ggaaaagtac cattctcgca atggttgaca gaaagagttg attttgcaac attttcagct
     6241 cataaattcc atggcccacg gggaactggt ttcatttatt ggaaaaaggg tcgtcgttta
     6301 gcaccattac tgacaggtgg agggcaagaa agaaatcagc gaagtgggac agaaaatgtt
     6361 ccaggtattg tggctatggt taaagcttta cgtttatcat tagagataaa ggaacaaaaa
     6421 cctgagcaga cacaacgctt aaaagtagct ctaatcaatg cgttgaagac gtatgagaaa
     6481 gtgacgattt tttcagaagg aactgactat gcaccgcata ttttatgttt tgctctaaaa
     6541 ggaattcgcg gcgaagttct cgtccatgcc ttagaagaaa aacaaatatt tgtttctact
     6601 actagtgcct gctcctcacg taaacatatg gcaagcagta cattgcacgc aatgcatgta
     6661 ccaaatgatc tggctacatc tgctgttcga atcagcttag atgaaaacaa ttcgcttgca
     6721 gaagtcgagc aatttatgat tattttcaag cagctttata agaaattttc tgcaatcaat
     6781 taaaagagga gtgttcttgt gaaatacagt gaaattatgg tccgttatgg ggaactttcg
     6841 acaaaaggaa agaatcgaaa aagctttatc atgcaactag cacaaaatgt cagacaagct
     6901 ttgaaagact ttccagaaat caaaatccat gctgatcggg accggatgca tcttttattg
     6961 aatggcgcag acgggcagct agttattcca aaactagcaa aaatatttgg gatccaaaat
     7021 ttctctccaa gtattcgtgt agaaaaagat gtcgaagttt tgaaacaagc tgttcaagac
     7081 atcatgaaag aaatttatac tgggaaagaa acatttaaaa ttatcgctaa aagaagcgat
     7141 caccaatttg aattgacaag taatgaattg aatcaaacat taggtaatgc tgtttttgat
     7201 attttcccgc atatccaagt tcaaatgcgt caaccagata ttccattacg tgtagagatt
     7261 cggcgtgatg gcgcttatct atcgtatgaa acgatcaagg gagctggagg attgcccgta
     7321 ggaacaagcg gaaaaggaat gcttatgctt tctgggggga ttgattctcc tgtagcaggg
     7381 tactttgcca tgaagcgggg agtggaaatt gaagctgttc attttgctag tccgccgtat
     7441 acaagtgaac aagcactgca aaaagcaaaa gatctaacag ccaaactagc accttatgtg
     7501 ggaacgatcc attttatcga agtgccattt accgagattc aagaagaaat caaaaagtca
     7561 gtgccacaag gatattggat gacaattact cgtcgaatga tgttgcgttt gaccgatgaa
     7621 attcgccgta ttcgtcatgg tttagtcatt ttgaatggtg aatcattagg tcaagtggct
     7681 tcacaaactt tgcaaagtat ggtggctatc aatgaagtaa cgaatacgcc aatcattcgg
     7741 cctgtagcga caatggataa acttgaaatc attgaagtag cagaacagat cgatacattt
     7801 aatctttcta tccagccttt tgaagattgc tgtacgattt ttgctcctcc acagccaaaa
     7861 acacgccctc aactggaaaa ggtccatcaa tatgaagagc gtttagcaat agatgaaatg
     7921 atccaacgag cactagctgg cttgaagatg gaagagatac aagcagaatc gtctaatgaa
     7981 aaaactgaag aattttcaga attgctataa gaggttatga aaatagtgtt ttgaccagag
     8041 tattagagaa acaaaacgaa aaaatagctc catgtatttt accgtttttg aactaatgtc
     8101 gaagattagc tgattgaata ccatttatca agaagttatg gaaaagccgg ttctttttta
     8161 ctaactgaaa aataaaattt tatttagaga tcaagctaca gttgataaat gatataaaaa
     8221 agagaatttc ccatgaaata gcgaggttgt gacagaagtg ggcagcttca agaaataaga
     8281 aggaatttac gaaaattgct tttcaaattt ttgtgaaatt tcggcttatt tttcgaagaa
     8341 gttacttctg gaacaccgtg tatcagaggt tg
//
LOCUS       DASNNU010000105         8248 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.105, whole genome shotgun sequence.
ACCESSION   DASNNU010000105 DASNNU010000000
VERSION     DASNNU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.105"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            235..1560
                     /locus_tag="U8116_002375"
     CDS             235..1560
                     /locus_tag="U8116_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN8435330.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1553..1903
                     /locus_tag="U8116_002376"
     CDS             1553..1903
                     /locus_tag="U8116_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435331.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1900..2070
                     /locus_tag="U8116_002377"
     CDS             1900..2070
                     /locus_tag="U8116_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435332.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(2974..3249)
                     /locus_tag="U8116_002378"
     CDS             complement(2974..3249)
                     /locus_tag="U8116_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEN8435333.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3221..4174)
                     /locus_tag="U8116_002379"
     CDS             complement(3221..4174)
                     /locus_tag="U8116_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN8435334.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4786..6279
                     /locus_tag="U8116_002380"
     CDS             4786..6279
                     /locus_tag="U8116_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN8435335.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6414..6710
                     /locus_tag="U8116_002381"
     CDS             6414..6710
                     /locus_tag="U8116_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEN8435336.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6811..7662
                     /locus_tag="U8116_002382"
     CDS             6811..7662
                     /locus_tag="U8116_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435337.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7683..7966
                     /locus_tag="U8116_002383"
                     /pseudo
     CDS             7683..7966
                     /locus_tag="U8116_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2864 a         1282 c         1552 g         2550 t
ORIGIN      
        1 atttttttct aatataacta agcaaaactg gataatacga cacttatttt caaattttca
       61 caagactaat agtaaatatc catttaataa agtgatacaa tgtgttgatt ttacaaatca
      121 acagaagaag tagggaaata acttacctat gcttcttttt ttgtacattt aatcgattct
      181 tctttacaaa aacgaacgta tgtttgtata atgtggaaga atggagtgtt atttatgatg
      241 gattattcaa aagagccagt aaatgattac tttttgatcg atatgaaaag cttttacgca
      301 agtgtcgaat gtatagaaag gaatttagat ccattaacga ctgaattagt cgtcatgagt
      361 cgagcggata atacaggatc agggttgatt ttagcttctt caccagctgc aaagaagaag
      421 tacggtattt ctaatgtcag tcgaccacga gatctaccgc aaccttttcc taaaacgtta
      481 catgtcgttc caccacgcat gaatctctat attaagcgaa atatgcaggt aaacaatatt
      541 ttcagaaggt atgtagcaga cgaagattta ttgatttact cgatcgatga atcgatctta
      601 aaagtgacca gatcaatgaa tctttttacg actgaaggaa caagaagtca acggagaaag
      661 aagctggctc aaatgatcca ggagaatatt aaagaagagc taggattgct tgctacagta
      721 ggtgtcggag ataatccctt attagctaaa cttgctttag acaatgaagc aaaacataat
      781 gatggattcc ttgctgaatg gacgtatcaa aatgttcctg ataaagtatg gaatattcct
      841 gaaatgacag acctttgggg tattggatct cgaatgaaaa aaagactcaa ccaaattggt
      901 atcatgagta ttaaagactt agctaattgg aatccataca ctattaagag ccatttaggt
      961 gtgattggct tacagctgta ttttcatgcg aacgggatcg atcgaacaga tatagcgatc
     1021 ccaccagaac cgacaaagga aaaatcatat ggtaattcac aagtgttacc acgagattat
     1081 accaggagaa atgaaatcga actggtggta aaagaaatgg cggaacaagt agctattcgt
     1141 atcaggcaac ataactgtaa aacaggttgt gtccatttat acattggcac gtcgatctta
     1201 gaaacgagac caggtttttc acaccagatg aagatcccta taacagataa tacaaaggaa
     1261 ttgcagaatt actgtctttt cttgtttgat aagtattatg aaggacaaga agtaaggcac
     1321 gttggaataa cctatagcaa gcttgtgtac actgattcgc ttcagctgga tttatttagt
     1381 gatccacaaa agcaaattga cgaagaaaat ttagataaaa ttattgacaa gattcggcaa
     1441 aaatatggtt ttacgtcgat tgtacatgct tcttctatgt tagaaggcgc tcgtagtatt
     1501 actagaagta cattggttgg tggccatgca ggcggcgcag gagggcttaa aaatgattga
     1561 ttataatgat cgtggacgtt taaagtgggc aggtttttat ttatctgatc atactgaaaa
     1621 aattgatgcc gacaatgaac aacgagccaa tcaaaatttg gcaaaagaac aaatgacgac
     1681 tgaagaggtt gcggaagtat tgaacgctgc agtcataaaa aatagatcag tatgtattca
     1741 aaaagaagaa cgaaacgctg aaggatacta tccagaagat attgttagca agatcttagg
     1801 tagtgatgag ctggggatct acgttggaaa aaacaaagtt cactacgacg aaatacgtca
     1861 tatcgaattt taccaggata ataaatgggg tcgattagaa tgaacattat ccagcaatat
     1921 gagctaaagt atataacctt tgatcagcta tcagaagaga tttggggtta tggccagcgt
     1981 ttgataaatg aggttggtgt tgaacgcttc tctttctatg tagaagctgc agcaggatac
     2041 cataaatacg aatactatat tggctattaa agtgcaataa aattatattt cttttatgtt
     2101 ttacattttg tatcaattct gataaaatta cctataggta attaaaaaat ataaaaaaac
     2161 tagttacaac cttaggacgt tgtaactagc ttgaagcata ctagagctaa ctaaaatagc
     2221 actagaaagc aatgttaaac ccgtccaaca aacttgattt aacattgaga ccagcttatt
     2281 cagctaaata tagttgaata agcttctttt gttttaaaaa aacaaaaggt gtactacgaa
     2341 taacgcaagt aaaagaaata ctattacaac cagtaggcta caagtaagta ttactaaaca
     2401 ttgcgtagtt agaacctttg ctctattgtc taaagccaca aaattagaga tcagctttat
     2461 gatgctaaga gcgagctgca tccatgcgga cataggttct tttgacacta ttaattctcc
     2521 tttttaggat aagggaaatt gctagtagtt ttttcattag caccatcccc cttcatttga
     2581 ttgagtctag tgctgctgtg atagtttgct gtttaatatg cttacattct tattataaca
     2641 taaatgaaac aaacacatgg ttagaggtcg tatcaatcaa aatagtgtct cacaaacgat
     2701 ttattactta ctcctgctga ctaacggact gttcttcagt aactgtaact atatgtttgt
     2761 taaaaccacc attacctgta gataaaaacc aatatattta ctatactcac tatattaaat
     2821 atatttagta tagtaaaaac tagaaacaat aaaatttgtg aatgttgtat acccccaaac
     2881 aatacacata ttttgaagga ttaggattaa taaagctaaa aatatagtaa gtatactaaa
     2941 tatatttact atatctagta tacttactat attttaatct tttgatcgtc tattatatat
     3001 aatccgttcc tcgtcgttta aagtatcaac ataggcgtcc attgcaagtt tgactatttc
     3061 atacatagga atatcttttg cataactaag atcccgaatg gtttttagta cgggaggatc
     3121 tacttgaact ggttttcttt ctttcgcagt gtactttcgt tcccctaact cgacattaat
     3181 atcatcttta attgaaatat cttcagcacc aattttcttc ttatctttgt tcgttggtaa
     3241 taatgccatt tagtttaatc tcctttccaa gaggtaggat atgattggcc attgagtcag
     3301 catatttagg tgtataatca aatccttcaa tatcaccatg ttgttcatag tattcaattc
     3361 tttcttctaa ttctgtaaag atgtctgcaa aaatagccca ccatcttcga tctacataat
     3421 catttaattg gataccattc tcaccataca tttctaaccg atccgagcct ttaacaatgc
     3481 ttttaaaaat gtttgattca ccatacttat caattgtttt ttgatagttt gcgtgttgag
     3541 aggaacgtcg cactgagaaa agaacaggca aaattccaat gatttgaaga tgagactctt
     3601 caaaattcgt gacaatcgga actaaaactt tattaataaa atcttctgtt ccttccattg
     3661 cgtaccgttt taattcttgc attgcaataa tataatctgc agcagttaag aatgcacgaa
     3721 ccacattatc tgttgatggt gggcagtcga taaagatata atcgaaattt tttctcaatt
     3781 cttctaaagg tttaatgaag gccaatgatc tcttacgacg atcaggatat ttttcggaca
     3841 agtattcatt aagttcttca gatccaggag agccagccat aatatataaa tgtggactta
     3901 cttgagtaat acctgaaacc cagtcccctt ttttgattgc tgcagacatg gatcgatcaa
     3961 agctagtaat ttgatacgtt gatccaatgt tggaagtagt atttctttgt tggtcataat
     4021 caattagcaa cacgtctttt tcaaaaagtt gactagaaac aatcgctaat aaatcattgt
     4081 ctgtagtctt accaatgccg ccttttctac ctgtaagcat tacaactttt cctagtttat
     4141 ctgatgtttt aaatgcagtc cgtaagtttt ccatagttcc ctccaatttc ctatactaaa
     4201 tatatttagt atacttcata tacttaatat attcgatata tctaatatac tccaaattga
     4261 taagaattca gactttataa ggaatatagt ggatatacta aatatatttg ctatattcag
     4321 tatatcagat atattcaaaa tgtcaaaata tatttgtgcg ttttgcgttg acaatttaga
     4381 gtgaaaagag gtaaacttaa ttctatagaa accacagata attcactatt tacaaagtag
     4441 caaaagcagt ggtactcttt aaacagaaaa attacataaa aaaaagagac ggaaataatt
     4501 gattttagca gaatcaatta tttctgaatc acatggttag gacatgtgtt cacgctcgtc
     4561 tcaagaatat gtaagtagct accaactact tatattaaaa gttgatcgga taccttttcc
     4621 gattgccttt attctaccaa aaaaaggcga gcaactcaac agaagaacgt ttatttagtg
     4681 tacttaaatt aaatattctt aaacactgaa gagagaacac atactatggc tgagattaaa
     4741 gctaaactgt gttctctttt tgtttgagaa aggacgttac aatcgatgtt gagcgaaatt
     4801 tattcagcta ttcttaaaaa cggcttacgt acatacaaat atcgaaacag caccatgaaa
     4861 cctgcaggtc atattgattc cagtaggagt ggtgctatct ttgcgtaccg tagcaagcaa
     4921 ttaatgaatg ttgggcgtgg tatggtcatt acttcggaag aagctatttt agagaacgaa
     4981 aagaaactaa cgcattggac acctaatacc tatcgctttg gtacttatgc ggatgattat
     5041 cgaacagtgg ttaaaggtca ttctgagaaa aacttaagtc aaatcaacac atttgtagtt
     5101 gatatcgatt caaaagagaa ccatcaaggc gaaattattt tagcctgttt agatcaagtg
     5161 ggctacatgc caacgctaat tttagaatca gatcatggtt atcaagttta ttttgtacta
     5221 aagacaccag gctatgtcac aaagaaaagc aatttcaagg tgatcgatgt agcaaaaaaa
     5281 atttcaaaaa caattcgtct acagttggct gaaaaattgt ccggagtgga tctaggatgt
     5341 aaccacttcg gaattgctcg attcccacaa gaaaataata ttgtttttaa agaaatgaac
     5401 tatcagtatt cttttgctga ctggattcaa tggtcgatga agcttgaatc agatcaaaaa
     5461 acaaaagaag atcgagaacg aaaactaatt gtctttcctg agaaaaaaga aattcgtcaa
     5521 gtagatgaac cgtggttcga tatgttactt cataaagcaa acatcgtcgg cggagaagga
     5581 cgtcttggtc gaaataatgt gattttcacg ctttctttag cttattactc ttcaggttat
     5641 gcattggata cttgcgaata caatatgttc acattcaacg aacggttaca ggaaccgcta
     5701 tctgaagcag aattagtaaa gattgtacgt agtgcttatt ctggtaatta tcaagcagca
     5761 agcagagaat tcattattga attgtgccag caatgggttt caccaacctt aaaaaataat
     5821 gatctgttca ttcaacgacg tggctggtgg aaatttaaaa agccacgaga tcaaagaaaa
     5881 tatagtcata aaaatgaatg gaaagaagat ctattggctt atctaagcga aaaaagtctt
     5941 gctagtgagc cttatttgaa cctgtcaaaa aaagaattgg cttcacaact aacgatgccc
     6001 aagcgaacac ttgataagat cttaaatgag ttacgagagg agaataaagt cttctacaag
     6061 gtaacacgag gaagaaatgg cggaatgatc attgcttcag tcaaaacgtt gttcgcgagg
     6121 gttattcaac taagtaaaga aaagagacta gattattcaa caaggatcac agaggtattt
     6181 ggactcaacg agagcttagt agaagagaca tttagacaat tatcaacccc cttgaaccct
     6241 cttaaagaag tgaatctttt tgaagtagat acaggttgaa aaataaaagg tgcaaattgc
     6301 cctatacaat atatctatga tacttctgta ggtgttgctc cctttgccta gctacgtgta
     6361 ggtggtatac ttataactgc gtatgcagtc ttatgtatca tggaggaagt tgtatgtcgt
     6421 taaaaaatta tgtctattca catcctgtta atgtgtttat cattggtcgt ttgggattat
     6481 ctgtggaagt gttctgtgag ctttatggtt ttaagcaggg gactttgtct tcttgggtta
     6541 ccagggaaaa aacagttgct tctttgccga ttgagtttct tcatgggttg tccttagctt
     6601 ctggggagac tatggatcag gtatatgatt gtttgtgtgt gttagagcag gagtatattg
     6661 agtataaaat agctaacgag ttaagaaaaa ggaagaagta tatccagtag tagtttttat
     6721 ttacttaaaa aattggttca ttgagcaacc tatcgctgct aaagtagcaa aggaaaacgt
     6781 taaaggaaag aatttaggga agaaatcgaa atgaaatcgg acgactggat tgaacacacc
     6841 tggcttcctc tgaaaagaaa tagcaaacga aagaaatttt gggtattgca attatggcca
     6901 ttaggaaaac tttgtagtta cggggcacga cttattcttg atttaaccgt ttactcgatt
     6961 ggagtatttg gaacggctta ttacttgata aatgatccca ataggattgc agcatcagac
     7021 atttttacag tgctaatgac gttgattttt gtcatgctat tgtctatttt tactcaatta
     7081 gtcatgcaat ttaccaggca aaaattaaca gcaggaaaac ttttcaaaag cctattcgat
     7141 ttaattgttg taaatgcaat tctagcgact ttaataaaac aaattgaaca atctggaaga
     7201 acaacaaaag gagtgaagga ttttttattg acgtttattc actttgtaaa tggacacatg
     7261 tatttgtttg aagtgggtat tattctcttt tttgtcgggt atgtatccac gaaacttatt
     7321 aaacgttcaa ttgaaaaaga aattgaaatt gacctgattc cgacacttct tggttatgag
     7381 tacaatgttg attttggaag atatgacgaa ttgatcaata cggatatcgt tcacacgagt
     7441 atccatgaat ctactgttaa gggcatcaga tctattttaa aaaatggcca ggaaatgaaa
     7501 gcaatagatc gaagaaaaga gatctacaat ttttatgaag tcgaagcaag cctacgatat
     7561 ggcatcggaa aattgtggtt agatggaagt aatattcgtc aaaacaaacc atacattttt
     7621 tggaacagta gtgatttaga acttccaatg aaagataaat aattatggag aatcgtttga
     7681 aaatgattcc tctatacagg attgtgctta tagttggcat ggagttaaaa accttaaaga
     7741 aggagcagtt attcaaagtg aaagaacgtc ataatcagtt actatgtagt aaaacgggta
     7801 cgatgaacat tgaataccga acagaatatt tagaattaac gaatcaaact gattttttgt
     7861 tgattcgaaa gcaacaagag caagaaaaaa attaatggct acttatagcc aattgattga
     7921 tgaatgggaa caattcaagg aatctaaaat tccagaacgt ctctaaaagc ctttatttgt
     7981 aaagagcata aggaaacgtg ggaacacttt ttaaaaaaag tgttccaata ggctgtgcct
     8041 attacgtttc tcaaaaataa atgtgggaac aaaatattta aaaataggat agtgaaagga
     8101 tagtgaagga tagtaaaagg agggtagtag gatagcaaaa aggataggct ccttttcgct
     8161 atccttttta aattggagga attaatatgg aagtaagaat aagaaatgtg gatgagaaaa
     8221 tcgtaagaga aattgatcga agatgtga
//
LOCUS       DASNNU010000106         7912 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.106, whole genome shotgun sequence.
ACCESSION   DASNNU010000106 DASNNU010000000
VERSION     DASNNU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.106"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..616)
                     /locus_tag="U8116_002384"
     CDS             complement(<1..616)
                     /locus_tag="U8116_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEN8435338.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKT"
     gene            complement(1044..1652)
                     /locus_tag="U8116_002385"
     CDS             complement(1044..1652)
                     /locus_tag="U8116_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEN8435339.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(1658..2689)
                     /gene="vanA"
                     /locus_tag="U8116_002386"
     CDS             complement(1658..2689)
                     /gene="vanA"
                     /locus_tag="U8116_002386"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN8435340.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(2682..3650)
                     /locus_tag="U8116_002387"
     CDS             complement(2682..3650)
                     /locus_tag="U8116_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN8435341.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3982..5274
                     /locus_tag="U8116_002388"
     CDS             3982..5274
                     /locus_tag="U8116_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN8435342.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(5372..6526)
                     /locus_tag="U8116_002389"
     CDS             complement(5372..6526)
                     /locus_tag="U8116_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN8435343.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVQITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            complement(6504..7199)
                     /gene="vanR"
                     /locus_tag="U8116_002390"
     CDS             complement(6504..7199)
                     /gene="vanR"
                     /locus_tag="U8116_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN8435344.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            complement(7413..7835)
                     /locus_tag="U8116_002391"
                     /pseudo
     CDS             complement(7413..7835)
                     /locus_tag="U8116_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         2230 a         1750 c         1427 g         2505 t
ORIGIN      
        1 ctgttttgtc ctctggataa cgtaaaatga acccgtattt ccaagcattt tcttctatcc
       61 actttccttc aggggctcgt tccattttcg tcaagcttga tcctacatct agtgataaac
      121 ctgaattatg ctcactataa cctgctggta aggcatactc agcccccatt tcttggtaaa
      181 gcacactttg ctcatcaaag tctcgatagc cactattaat aataaaatga ctaacgccac
      241 cctttacagc atcattgacc atctctgaaa atttttgtgc tatttctttt gacatataaa
      301 tattactatc aagcaacccg tatccattta ttaattcgtc atgtttagat aaattcacga
      361 tatctgactt cacactttct tggcgaacag gatatttact attgattaat agcagatttc
      421 cttgataaac ctgttcttct gtaattgttt tctcttgggt attttcagaa gtcccactat
      481 tttctaaatg ttctttggga ttttgatcat aattttgaaa ttcgactttt tcctgagaaa
      541 acagtgcttc attaacgtag tcataaccta agtatattaa gaataacaat aacaataaaa
      601 aaaacaactt cttcattttc agtctcctcc ttaactatat agttggaagt atgaaatatt
      661 tctagaatct tccccttaaa cggcacaatc tcgtttgaga ttgtgccgtt ttgccccgta
      721 aattacagtc aattatttcg ccctactgga tttactatta tcaccaatgt agatgaattt
      781 tcataagtga tacccatgct gaatttaacg aggctgaact gcctgttgac aatgggcggt
      841 gtgaaatacc acccctctat gcgatcagtc ttattatcag gctatcttgc cgcagcgcac
      901 gcagataatg accgcaatat cgctctgcct tacatcttca atactatcta ctatatataa
      961 attccctaag aaccagttac atacgtcggg tttcctgccg aaagagttag cttatatagt
     1021 ttgtccgtgc aacggttaaa agtttattta acggggaaat caaaatagct attggggtat
     1081 ggttcgtctc ttaatacata gtgccaccat tcgaggctat atgcttcaaa cccactgttt
     1141 tccatgatgg agcgcaaacg tctgcgattt tgcgcttcat tgcatgatat tccatttgcc
     1201 gcatgatgag agcgttcatc cataaaatca aatcggctcc ccattggtac aagctcaccc
     1261 gtgtctaatc gataaagcgt aagatcaatg gcactgccgc ggctatggct tgattttgaa
     1321 gccacgtatc cttttgaaat catctcagtt cggtcaatat tgggataata actttccttt
     1381 gtcaggttat tttccggctg tgcagcccat tgcataaaac agtttacagc acgattagga
     1441 cggtaaccgt cccatagaag caatccgtac ccttgggtag cagccagttc ttttgccttc
     1501 aaaagcgatt cagccaactc gtatgtccct acaatgcgat ttacttcata accgtcaacc
     1561 ggttttccgg tgaaattatc ccaagtggca tatttagcgt cccaacgaac accgtgtact
     1621 atttcatcta aaaaagtaaa tcctatttcc atgcttatca cccctttaac gctaatacga
     1681 tcaagcggtc aatcagttcg ggaagtgcaa tacctgcagc ggccatcata cggggataac
     1741 gactgtatga cgtgaaaccg ggcagagtat tgacttcgtt cagtacaatg cggccgttat
     1801 cttgtaaaaa catatccaca cgggctagac ctctacagcc gagcgcttta tatatttttt
     1861 ttgccgtttc ctgtatccgt cctcgctcct ctgctgaaag gtctgcggga acggttataa
     1921 ctgcgttttc agagcctttt tccggctcga cttcctgatg aatacgaaag attccgtact
     1981 gcagcctgat ttggtccacc tcgccaacag ctaacgcggc actgtttccc aataccgcac
     2041 aaccgacctc acagcccgaa acagcctgct caattaagat tttgctgtca tattgtcttg
     2101 ccgattcaat tgcgtagtcc aattcgtccg cgctattgac ttttttcaca ccgaaggatg
     2161 agcctgaacg cgccggctta acaaaaacag gataggtaaa cgtagctgcc accggcctat
     2221 catctttatt aataacccaa aaggcgggag tagctatccc agcatttttc gcaacgatgt
     2281 atgtcaacga tttgtccata caaattgctg agctttgaat atcgcagcct acaaaaggga
     2341 taccggacaa ttcaaacaga ccttgtatgg atccatcttc acctgacttg ccatgcaaag
     2401 ctgaaaatgc tacatcaaca tggttgattt catattcatg gttcttttta acaagtaatc
     2461 cgtgcatttt tttatccggc gagagtacag ctgaatagca attgtcgttt tcccattccg
     2521 cgcaaggttt ttcgcacatt ttccatacac cagatttcgt aattccaatg tataacggct
     2581 cgtatttttc tttattaatg ttagcggcta tctctattgc agattttacc gatacgtcat
     2641 gctcctctga gcaaccccca aacagtattg caacttttat tctattcatg ctcctgtctc
     2701 ctttcaaaat ccaaacagtt tttaatggtt ttttcaacgg tatcacgcaa cgcttgctcg
     2761 gtataatagg ccgtatgcgg tgtgattatc acgttaggca ttctttgaag tttaagtaaa
     2821 aattgattat caattggttt ttgggtgcaa tcagagtaga aaaactcttc ctctccttcc
     2881 aatacatcca atgcggcacc gcccagtttc ccgttttcta atgctttaac caactcatag
     2941 gtatctacaa gtggaccgcg cccagtattg ataagaaatg ctccttgctt cattctctgt
     3001 atttgttcgt ggctgataat atagtgcgta tccgtattga gcggcacatg aagcgtaacg
     3061 atatcgctat tttgcagcaa ctcatcaaac ggtacatagt ttacctctat acttcggctg
     3121 cgactataag ccaacacttt acatccaaat cctcgcagcc gctcaataac cgctttgcct
     3181 atctggcccg ttcccaccac accaactgtc atgtcgctga gtaccttgcc acggtcgctg
     3241 tccaacctga aatcatgttt ttccacagag cgcacaatcg attttacgtt gcgtactgcc
     3301 ataagaatta gcatcatagt ataatcggca acgctatccg gcgagtacgc cacattgtcg
     3361 acagtgatgc ccattctctt agcagcagtt gtatctatat gattgcagcc gatgcttcgg
     3421 gtagaaatat atttcacacc ggctctcttc agcgcaagaa gaatagaggc ggaaatctct
     3481 gatttatgtc ccacactgat acattgattg aaaggcgcgg atttggcgtt ggattccgac
     3541 acgttggcgt taattatcgt tgccataacg ccaaagcgag gcgaaagagc atggaatgca
     3601 tctgcctcat cctgctcaca tccataaaca gtaatgccga tgttattcat aattaagacc
     3661 aaccctttct gtgaaaggca ccaaataaaa cggggatgcc aatggtaatc ttgtcaaatc
     3721 tttctctgaa gaacgaaaac ggctcgttct tacggtatta agtctaccgt gtaagaacga
     3781 gccgtttctt aatattaaat tgttaatttc ctaagaaaaa gtaaagataa ccttgagatt
     3841 ttcctaaaaa attatggctc tatacttttt gatgttggtg gatgacacca gcatcttttt
     3901 tattttccaa aacatataga aaagacacct tgaaatcctt tagaattaat ttggcgaacc
     3961 aacccaaaag gaggaatcaa gatgtcttac actcacctta tcaaagaaac attagatatt
     4021 ctagacttat cagttacttt taacgaaaat tgtctcacaa aagaaaaata caaagggcaa
     4081 atctgtcata tttatcgtgg aaacttgatt tatacagccc aagaatgcat ccactgtaaa
     4141 caccagattg cttcagatat tgtgcgttgg ggtacaacaa ccgtccggtt gttaatgaac
     4201 gatgtttctg aataccgtac ctacctcgaa ttgaaaaagc aacgcttcaa atgcaaagct
     4261 tgtcagcgga cctttgtcgc agatacttct gttgcgaaaa aacattgttt catcagtgag
     4321 aaagtccgct ggtctgtcgt gactcgtctg aaaaaaaata cctcaatgac agagatcgcc
     4381 gctcagaaaa atctatcggt ctcttctgtt taccgtatta tgaagcgatt ttatcgtccg
     4441 ttgaatcctt ttcggacacc gttacctaag gtactttgtt ttgatgaatt caaatcagtt
     4501 cggggtgttt caggtgctat gagtttcatc atgatggatg gacaaacaca gcgcttattg
     4561 gacattgtcg agaaccgaca actgcctttt ttaaaacgtt attttagtca tttttcacga
     4621 gaaattcgtg aagcggtcga atggatcgtg attgatatgt acgctcctta tgtatcgctc
     4681 gttaaaaagc tgtttccaaa agcacagctg attattgacc ggtttcacat cgtccaacat
     4741 attggtcgta cctttcgcaa tcatcgaatc aaagaaacga accaactatt aaaaagcaaa
     4801 gaacaaaaac actatcaatt aggtaagcaa ttaaaaagat actggaaact tcttcaaaaa
     4861 gatgaacgca agcttgatta tactcgacgc ctttggcgac ctggcttcaa agctcattta
     4921 acagagaccg atattgtgga tcgcttactg aaagggagcc ctgctttgcg tgtaggctat
     4981 cagttatatc aagactttct ttacgctgta aaggaacgag actacgtatc gtttgaagaa
     5041 ctacttacca ataatatcat gcttcccgaa ggctatcaga cgaccttgag aacgttccag
     5101 aaatttctgc cacaaatcaa aaatgcttta caacaaagtt attcaaatgg cccacttgaa
     5161 tgtctgaaca accatatcaa agtgttaaaa cgaaatgcat atggctttcg tagcttttat
     5221 aatttcaaat tgcgcatcat gattcgtcat ggcaacgcat tgattttcaa ctaaaaaaga
     5281 tcaagaggaa ctgatccact tgatctccaa ttattctaag atttctcttt accaacatca
     5341 tttgacaaag agccaaaatt atatacatct cttaggacct ccttttatca accaagtctg
     5401 gcatcgctgg aagctctacc ctaaacgtcg tatagttatc attgctttcc gcgtaaatct
     5461 gccctccatg ctgaacaata atttcttttg caatcgccaa tccaagtccc gcgccacccg
     5521 tatcggaaga acgagcattg tccagcctat agaacttttc aaatatggca gctagcttat
     5581 cttttgggat gcttccagtg ttcttgaatt cgattgacac cacatccccg gagaggcccg
     5641 cggtaatgtc aatgatgctg ttatcctcac tgtatgcagc ggcgtttttc aaaatgttgt
     5701 taaagactct cgcgagttta tcagggtcgc cggacacggt cagatcctcg ggggcgtgaa
     5761 taaccgcctg ttttccatgt gcggaaagct gaggataaaa ttcatcggtc atctgcacca
     5821 gcatatagta taggtctatg tgcgtttttg ttagcgttat cgtttgtagg ttataccgtg
     5881 taatctcaaa aaactcgtcg attagctgtt cgagtcgata cgctttgtcc aacgtgattt
     5941 gcacatactt tgccttttga tctaccggca tgtctggagc ctcgtcaagc aggctcaaat
     6001 aaccgataat ggatgtaagg ggcgttttaa tatcgtgcgc caagtacata acaacgtcat
     6061 tttttctttg ttcggccagc tttgcatcct gctctcgctt ttccagagtc cgttttaatg
     6121 tgttgagctt ttgttccata acatccattt ccgcagaaag ctcaatttgt ttatcttcgt
     6181 tctgaataag tacatcaatg ccggtattta tctcgtcaaa gtattttgcg aattttgaaa
     6241 gcatgacgcg acatagaata agaatactaa tgacaatcgc cacataaata aagatatcta
     6301 tattgttccg tatggaatat tgatataatt tcatcgcgtc caggtgattt aagtcatatt
     6361 tgttttccaa aatacttaag atccaatccc caagtttccc tcggatcatt gaacgaatat
     6421 acaacacgaa tacaattgct accacaacaa ttgcaacgat atacatgtaa agttttcgtt
     6481 ctagtttgga atagtcgttt tttttatttt tcaattttat aaccaacccc ccataccgtt
     6541 tttatatatt tcggattatc aatggtgtcg ttcatttttt cgcgcaaatg ccggatatgc
     6601 acggtgatgg tgttgttgct cttgctgaaa tattcgtcgc cccatatctc atgaaatagc
     6661 agctcggagc taaccacatt ccccttgttt tcacagagga ttcgcagtat tgaaaactcg
     6721 gtgggagtaa gggataactg cttctcgttc agataacact catgggtgtt aacattaatg
     6781 acaaggccgg agtggacgat aacattttcg ttctgctcct ttactccact gaattttttg
     6841 tatcggcgca actgggcctt tacccgagca attaactcca gtgggcgaaa gggcttcgtt
     6901 atataatcat ccgcgccgat tgttaaccct gtaattttat ctacctctgt atctttcccg
     6961 gtcagcatga taatcggata ggtgtgcttg tcccttattt tttgacagat agtaaggccg
     7021 cttgtgccgg gaagcatgat gtccaatatg gcaaggtcaa tctcagactt gtctatacat
     7081 tccaatgctt ctttggcggt atagtatttg aaaaccgtat aattctcgtt ttttaagtat
     7141 aattcaacca aatcggcaat ttcatgttca tcatccacaa taagtatttt atcgctcata
     7201 gttatcaccc tttcacataa gtttgccctt aataatttat cagattatag ggccgaattt
     7261 ataagcattt acataataaa tgactaagaa tttcttaaga tgatgattct ttttttatca
     7321 aagggctttt cggcatcgct aggaggctgt taatatttta tagtttcctt ttcttcttta
     7381 aaaatattgc ccattagcgg aatacagaat ggctaattat tcacttctga taatttcctg
     7441 tataatgaag ccctagatac attagtaatt tcacaaattt gatttacagt catatttcct
     7501 tctttatata gctttaccgc ataattcatt cctgcgtgat ttttatgata cttctttaat
     7561 cgacctttaa actttccttc tttcttagcc aattcaatcc cttcacgttg tctcatccga
     7621 ataagatctc gctctaattg gttaacacca gccattacag taattaagaa ttggctgtat
     7681 ggattatctt ctgataaatc aagccatgta tcttttagtg attttaaact tgccttttta
     7741 tctcgtatgt tatcgattaa ttcaaataga tcttgtgtac tacgagtgat tcgagttaag
     7801 tctgtaacat aaatgatgtc atcttcctgt aggttctgtt gcaaagtttt aaatctacta
     7861 tcaaataagg tagaataata gaaaaagata gcaggaggaa tgacgatgaa tc
//
LOCUS       DASNNU010000107         7823 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.107, whole genome shotgun sequence.
ACCESSION   DASNNU010000107 DASNNU010000000
VERSION     DASNNU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.107"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..642)
                     /locus_tag="U8116_002392"
     CDS             complement(<1..642)
                     /locus_tag="U8116_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEN8435345.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTK"
     gene            complement(644..1408)
                     /locus_tag="U8116_002393"
     CDS             complement(644..1408)
                     /locus_tag="U8116_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEN8435346.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1416..2093)
                     /locus_tag="U8116_002394"
     CDS             complement(1416..2093)
                     /locus_tag="U8116_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEN8435347.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2130..3515)
                     /locus_tag="U8116_002395"
     CDS             complement(2130..3515)
                     /locus_tag="U8116_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN8435348.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3725..3809)
                     /gene="ffs"
                     /locus_tag="U8116_002396"
     ncRNA           complement(3725..3809)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U8116_002396"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3832..4347)
                     /gene="tadA"
                     /locus_tag="U8116_002397"
     CDS             complement(3832..4347)
                     /gene="tadA"
                     /locus_tag="U8116_002397"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEN8435349.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4454..5311
                     /locus_tag="U8116_002398"
     CDS             4454..5311
                     /locus_tag="U8116_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8435350.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5392..5727)
                     /locus_tag="U8116_002399"
     CDS             complement(5392..5727)
                     /locus_tag="U8116_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435351.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5794..7164)
                     /locus_tag="U8116_002400"
     CDS             complement(5794..7164)
                     /locus_tag="U8116_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEN8435352.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7242..7778)
                     /locus_tag="U8116_002401"
     CDS             complement(7242..7778)
                     /locus_tag="U8116_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN8435353.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2227 a         1666 c         1268 g         2662 t
ORIGIN      
        1 ttttgtttgt ttcaagctat tttttcctaa gaaaagaaaa acagcaaaaa ataattctaa
       61 taatacaaaa aataagataa agctatgcat gaaaaattct tctggaagta cattgatgat
      121 tggatcagta acgggatcga ataaccagtc atcgttgttg aaaaacgttt catgaaaaag
      181 gataaaaaag cgatcaaatc ctatcatcat gaaaaaaccg aaaacaactg gcagtaacat
      241 gccgatttga aacggacgaa tcaaccgcca aagccgatca ttcttttgca aatatttgat
      301 aaacaaaata ctcgggatca aagtgatgaa aaacacagca taatccacta aaaacaaaat
      361 tttcacttca taaaagtgat gggctccgct agcagaaact gggaaatctg gcaagctgag
      421 gatggttttg aagggattat tgagataatt cattaggtga tcaaaatttt tcagtaacgt
      481 ctcttgatct aaagaagtat aatccaaaat ttgcaaatgt ccgatatcaa atacgtatag
      541 tggtcgaaag ttgatcgtca caccaactgc cagactgatc agtgtcagaa ataggctgaa
      601 caatccagca tattccatcc actgccatct cgaatttttc atattagaag ctccattcat
      661 ccagtgattg tttcacatag gtagctggga taggcaaccc aggtacatct tcttcttttg
      721 taaaacctga taaaacgagc aaagtatcta tattattgcg tatcccagct tggatatctg
      781 tttcatagtt atctccaacc atgattactt cttctttttg cagccctaat acttcaacgg
      841 ctttatccat aatgatggct tctggttttc cgatgtagat cggcggcgtt tgggttgctg
      901 tctctacaaa agagatgact gaccctgctc caggaagaag gccgcgttca gtaggtatat
      961 tcttatctgg gttcgttcca atgaaagtag cacctttttg aatagcaagt gttgctttaa
     1021 ctactttttc ataggtcaga taattatcta agcctaccac tacatagtca ggagcctctt
     1081 cttcccatgt aaatccggca gctaaaatca agtcaatcaa tcctgcttct ccgataacat
     1141 agactttttt cccttttcca tctgctttca taaaatcgat tgttgccaaa gtagctgtgt
     1201 agactgtctc tggagctaca tgaatgtcaa actcatctgc tagacgatta gctactgttt
     1261 ctggtgactt agtcgtgtta ttggtaacaa acaagaaagg taattttctt ttttgcaatt
     1321 cttccacaaa tcgtttaccg gccggtatgg gttgtgtccc acgataaatc gttccgtcta
     1381 aatcaattaa atatccttta tagtccattc atccactact cttccctttt tctgatcgta
     1441 aatgctgttt ttgttttttc tacttgttta ggttgttctt gtttttttac tttttcagag
     1501 gtttttgctg actctgtttt tcgattttta ataaccggtt ttttgcgatt agatgcgact
     1561 ggtccttttt tctcatcgat atacgcacga ttatggtttt ttttcttttt tcggcgctga
     1621 gttgtctttt cttttttacc gccgattctc tcgatgacaa aatacgcaca accaaaattg
     1681 caatattcgt aaagatagtc ttccaaggaa tcgattcgct gatctggatg cgcccgtcga
     1741 ttatccgctt ggaaaaatcc ttttaggcgt agttgttcat aaccccaatc tcctacgata
     1801 taatcatatc gggctagtac atcactgtaa cgttctccaa gcttttctgc attaaatcct
     1861 tcacgataat cggacacaag ccgataatgt ctttcaccta tcaaaaaatt actgtcatct
     1921 aacagtgtca ctagctcgcc tttgactttt tctgctttag gctcctcaac gatttcttcc
     1981 aaagcagctg tcagttcttc ggtgactgtt gtttcctgtt cagctgatga ttttttctta
     2041 gcaatttctg ttttatgctc aacgttctgc tgttcatttt gttttgcaga catgcgttca
     2101 ccacctatct tttttattat accttgtttt tatttgattg ggtagtgagc tttcaagtaa
     2161 tctcctacca cacttcggat aaattcaggg aacaaaaatt cattttttcc agcaatctca
     2221 atcgttggaa aaaatggaac aaacatcaaa tgatctacgg tagtaaaagt ataattttta
     2281 ttcagatcga ttggcttgtt cttccaaaag acttgatggt taatagaatc atatgtgatt
     2341 ccattataga ccatctgccc aaaaatctta ccgcgaaatc ccattccctt cataggatag
     2401 ttacgtaaaa agttacgatt tttttctatt tctaaaacta agcggatcag atcatttcca
     2461 cccaatgtga catttaacaa gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga
     2521 ttgacccgcc cttttggaat ttcagttaaa aacagaccac tgtttaatat tgctgcttcc
     2581 gtctttcctc gttctttcac cgcctcaaag gctacttgga taaacgaatg ctcattgaag
     2641 atatctagac tcagagaaaa tggcaaatct gctacttctt tttcttttaa taattcatgc
     2701 ccacgttcta gataactatt tatttcttgt tcatcttctg taaaagctgt cattgtttcg
     2761 gtagggatgg ctttggctga atgcttaagg atttttttct cttcatcgat ggttaaatgg
     2821 acttcaccga catactgccc gtactttcca gcggctgcta tttggacatc attgattacg
     2881 tgaccttctc tgaataaatg atgggtgtgc gatcctaaaa taacatcaat tgctggtatt
     2941 tcttttgcaa tttggaaatc atcctgaatc ccgagatgac tcatcaaaac taaaacatcc
     3001 acttgtgaac gtaaatcttc gactaattct ggcaaaatat cataaggatt ccgtatgtcc
     3061 cagccattcg gattataagt aagcggaaat ggagcagtaa atgcaattag tccgattttc
     3121 gtctggtgtt tcgttgtaat gattttatat ggtttcgtcc atgccggtgg ctggagcgta
     3181 tttttatcga ataagttatc caggatgata tcaaactcag catgatcgta taaatgattc
     3241 aactcttctt tggaatttcc tacgccttca ttgtttccta tcgtcgctgc atcataacca
     3301 acttcattca tcagcaagat attttcctgt ccattcgttg cttctgacaa tggatgccaa
     3361 cgatccacaa aatcacctag atctactgca atcgttgtcg ttttttcttt cttttcgttt
     3421 tctcgtttac gttgatccaa aaaccttctg atccttggcc aattttctaa atgagaatgc
     3481 agatcattcg tatgtaaaat cactattttt tccatgtacc ctctcgcctc tctttatctt
     3541 tatcatacca taggcagctt tttcttccat tgaaacaagc tctatttcag agtttttcct
     3601 gccttgaagc aaattgtata agtacaatat tcgataaatc agtctggcta aaaaatcagg
     3661 aacattcatt tttacagtac tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt
     3721 ttcattataa tcagacacat ggcggaacct gcttagtgct gcttccttcc agacctgaca
     3781 cgcttcacaa gcccaccatt gtcctagcct ttcggcaaag aatagtatac cttatttttc
     3841 attcgttgac cagtcttttt ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc
     3901 agtcaaaatc atcccacatt cgtcttctaa aatgccacct tctacatatg cttggtggtt
     3961 aaaccgctcg tcctccaaaa gattcatcaa ggtaccggct gttccgcctt ttggatcata
     4021 cgcgccgaaa taaacttctg ctacacgaga caaaatcatc gcgccgctgc acataggaca
     4081 aggctctaag gtaacaaaaa gtgcagcatc ttccaagcgc cagcttccca ctttttcaca
     4141 ggcttccttg attgcaagca tttctgcatg tgtagtagca tcttgcgtgg tttctctaag
     4201 gttatatcct cttccaatca cttttccatc caatacaacc actgctccaa tcggaacttc
     4261 atgaagcatt tctgctttct ttgcttctgc caatgcaagg cgcatccatt tttctttttc
     4321 tactgcatca agatttgtat ttaccaatta cccttccctg cctttgtttt ttcttgtttt
     4381 actttagcat atgataaaaa ggctgtaact aacttttttt tctatggtaa aataaataca
     4441 agaggtgaac aagatgaaaa tggataaatt acttgctctt tacccacaag cacagaaaaa
     4501 aaactctcca gcatcagaag cggatatcct ttcttttgca attgatgatg cttttatctg
     4561 gatcaaacaa gattcattat ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt
     4621 aatcaacgat caaaaaaaac acccatggta tcgctatctt tttaaagatg cttcttgtgt
     4681 tcaagaaaaa agctttcgtt tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa
     4741 aaaagaatgg caagatacta ttttggaatt atttcgctat tctgctgatt tcttttttta
     4801 tacagacaca gatgcgctct tagtcgaaca aataaaccgt gattatcttg atgctactga
     4861 aatcaacggt atttttctat ctttagatgc tgattttgat acgacaacgt ctgtattcgt
     4921 tggttctttc cattcacctg gcaccgacct cgtccctttg tttgctgaag aaagaaaaat
     4981 ttttttaaca gagcaaaaca atctttatac tcacagccgt actttttcta tgcaagatgt
     5041 ggctttacat tactatacaa aagatactgt aaaagatagt aagttgatca aaagttacat
     5101 agaactgatc aaacaaaatg aaatggaagt gatcattcaa gaattatgga atgaattagg
     5161 caatgtcagc tctgctgcaa aaacactgtt tcttcatcgt aatactctaa aatatcgaat
     5221 cgaaaaattt caagaacagt ctggtttcaa tctaaaaaaa gccaacgatt tactgttttg
     5281 ctatctttta ttgcttcatg aacataatta aagatagatt aaaggggttg tgacaataat
     5341 tttgtcacaa ccccttattt cgaatgaata gtgttcaaaa gctagtttct cttacatcat
     5401 tttttcaaca aaatgcatta gccggtcttt tacatcttcg gttgaatctt tgattgaatc
     5461 accataaaca gagattttct tttttcctga tttgattttt gctaacattc ccatcaaaga
     5521 atccaagtca ctatctgaaa gaccatctac gatttctagt aatttttcat ttccatcaaa
     5581 tttgtcatag acgaattttt ttactttcgt ccgagtagtc atatgatgga tcttttccat
     5641 tacttttccc gatacaacga ctgctgtcgc tacactggca gcggctgcta cgcttaatcc
     5701 gattgttact tttgttgatg ttttcataag tatcagctcc tttttatcta tctccatcat
     5761 agcatagaca aaaaaataaa cgaactaatc cactcagtga aatctcaatc ctttagggaa
     5821 aaagttcttt actgttcctt ttacttgttt cccaaatccg acaggtatct tatctaatac
     5881 tagcaaaacc catccttgat tcccatcacg ttgaaatgtt tctcctgctg tgtaagattg
     5941 ccattccttt tgagtgatcg gcaagcaagg tatattctct atcttttttg tagcaagcgc
     6001 taatgcatag ctaggttcaa atcgattctt tttgaaatct cctaaatgca aacctgtacg
     6061 tacgactttt aaaccatcga gagaaggggc taactcagga acttcccaaa gatggtcgtt
     6121 gaacactaaa agtcttccag tagcttcata atgaaaatca tttgaaaact ccgtccagag
     6181 tttttcctgc tcttttgtca tttgcacttt actttttttt cgtgtcttct tttctttatg
     6241 catctgattt tgtccatgaa aagtcagctt tgcaacaaaa tgtccttcac cctgatcttt
     6301 atgaggccat atacgtattg ttttttccaa accagctacg cttccccatt cagaacgtcc
     6361 tgaactaacg gattgagtca aaggaatctc ctcaatcgtt actggataat tttctacaag
     6421 ccagctaatg atctcttcat tttcttctgg tgcaaacgta caagtcgaat aaatcagttg
     6481 tcctttattt tttagcattt ttattgctga agacaaaatc tcttgctgac gtttttgaca
     6541 gtatagcgga gattcttctg tccactcttt tattgcattt ggatcttttc ggaacattcc
     6601 ttctcctgaa caaggagcat caaccacgat ccgatcaaaa aagcccgaaa aatgagggac
     6661 aagttctgcg ggtgcatgat ttgtcactat tgcgttcgaa actccccatc gttctatatt
     6721 ttctgataaa atcttcgcac gtttcggaaa gatctcattc gtcactaata accctttccc
     6781 cttcatttga gcagctaatt gggttgattt accaccggga gccgcacaca aatccaaaac
     6841 tttttcaccg ggttttgctg cagcagctgt tccaacgatc attgcacttg gttcttggct
     6901 atattcatac ccagcttgat ggaggaaaga ctttccattg acagtgccaa gaaatccctc
     6961 attgctgtag ggagcgggtt gtaattcttc gctatggtaa gtttggacca tgtccaatcc
     7021 tgccggtttt agtggattcc accgaaatcc ttttttcacg cttccttgtt caagcgcaga
     7081 aaaaaaatcg gatgcttctt cgcctaaaag aagacgatat tttttgataa attgctgtgg
     7141 taacgttgtc gcttcctcct tcattccaat gtttcgttag acattttaca atctttttta
     7201 cgaaaaataa aggtggctga tctacttttt catgttgctg cctaaggtga ctgttccttt
     7261 aagatatttt ccaagtattc atccgtcacc tcgtttttat cccaaacagc gttcatcatt
     7321 tcaagaaaat cttgagaaga atcatctgag cgcaaacttc ccaatgcatt aagccatacc
     7381 aattcaagcg acttttcttc tttgatcttc gtgtgacctt tgacatatga gaaaaatggc
     7441 cgcttaaata gcgaatccat gtctaaatca gattttgcaa tgatttcacg aatactttca
     7501 tggatagaaa aaaaatcttt caataagtag acaatgctta aaacatcatc ggaactgcat
     7561 tcagctaagt agttttcaaa ataaacagtg atttcttcta atatcttctc ttttaacccg
     7621 ctgattgatt tgaaatgtgt ataaatcggg tatgtagaac aaccgcagaa ctctgctaac
     7681 tctcttgcag taattgactt cattccatat tcttccacgt ataccatact tttgaacagc
     7741 aaatattctt tactataatt tgttactctc cccatcattc ttacctccgt ctcttttcat
     7801 ctacccataa ataaataaca taa
//
LOCUS       DASNNU010000108         7760 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.108, whole genome shotgun sequence.
ACCESSION   DASNNU010000108 DASNNU010000000
VERSION     DASNNU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.108"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="U8116_002402"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="U8116_002402"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEN8435354.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="U8116_002403"
     CDS             complement(2032..3285)
                     /locus_tag="U8116_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN8435355.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="U8116_002404"
     CDS             complement(3291..4214)
                     /locus_tag="U8116_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN8435356.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="U8116_002405"
     CDS             complement(4435..6927)
                     /locus_tag="U8116_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN8435357.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="U8116_002406"
     CDS             complement(6934..7401)
                     /locus_tag="U8116_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEN8435358.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="U8116_002407"
     tRNA            complement(7537..7610)
                     /locus_tag="U8116_002407"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="U8116_002408"
     tRNA            complement(7668..7741)
                     /locus_tag="U8116_002408"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DASNNU010000109         7741 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.109, whole genome shotgun sequence.
ACCESSION   DASNNU010000109 DASNNU010000000
VERSION     DASNNU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.109"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(177..1427)
                     /locus_tag="U8116_002409"
     CDS             complement(177..1427)
                     /locus_tag="U8116_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEN8435359.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(1805..3255)
                     /locus_tag="U8116_002410"
                     /pseudo
     CDS             complement(1805..3255)
                     /locus_tag="U8116_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            complement(3576..4340)
                     /locus_tag="U8116_002411"
     CDS             complement(3576..4340)
                     /locus_tag="U8116_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEN8435360.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(4368..5066)
                     /locus_tag="U8116_002412"
     CDS             complement(4368..5066)
                     /locus_tag="U8116_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEN8435361.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPTPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5078..5857)
                     /locus_tag="U8116_002413"
     CDS             complement(5078..5857)
                     /locus_tag="U8116_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEN8435362.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(5873..6817)
                     /locus_tag="U8116_002414"
     CDS             complement(5873..6817)
                     /locus_tag="U8116_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN8435363.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6863..7642)
                     /locus_tag="U8116_002415"
     CDS             complement(6863..7642)
                     /locus_tag="U8116_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435364.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2164 a         1637 c         1272 g         2668 t
ORIGIN      
        1 ttacttgtct catgtagtac gttttttcgc cttttgactc agctgaatat tctgtctgtt
       61 ctgttataac aaaactctca tacttctcca tcagtggttt taacatcatt aactgttcat
      121 aatgcccccc agaggaagcc gcaaaacata tctttttgtt catattaacc tctctatcag
      181 tcacgctgaa aaatatctct agtgtaaact ttttctttaa tatcatccaa gcaggtatca
      241 tatcgattgg caataataga atcacacatc gccttaaact gctctaaatc gttgataacc
      301 ttacttccga aaaaagtctc tccatctttc agtgcaggct cataaatcac cacagtcgca
      361 cctttagctt ttacgcgctt cataactccc tggattgaac tctgacgaaa attatcactg
      421 ttgctcttca tggtcagccg gtatataccg attaaaactg gattttccct gttgacatca
      481 tactgattat attcagcgta gttgtaatag cccgccatct gaagaacacg atctgcgatg
      541 aaatccttac gggtacgatt agattcaacg atagcctcaa tcaaatttcc aggaacatct
      601 gcatagtttg ccaacaactg cttggtatct tttggcaagc aataacctcc atatccaaag
      661 gatggattgt tgtagtgagt tccaatacga ggatcgagac aaacaccatt gattatctgc
      721 tgagtattca gccccttcat ttcagcataa gtgtctaatt cattgaaata gctgactcgc
      781 agtgctaaat aggtatttgc aaacagcttt actgcttcgg cttcagtgaa tcccataaaa
      841 agagtatcca cgttctcctt aattgcactt ttctgcagaa gtgttgcaaa aacatgagca
      901 gcatttaaca agcgctcatc agtcatatca gtaccaacaa taatacgaga tggatacaga
      961 ttatcatata aagccttgct ctcgcgcaga aactctggac taaaaataat gttcttactc
     1021 cccgttgtct cacggactga ttcagtgtag ccaacaggaa ttgtagactt gatgaccatg
     1081 atggtatcag gattcacgtc catcacaacc tcaataactg actcaacagc agaagtatcg
     1141 aaatgctgag tacgactgtc atagttagtc ggtgtagcaa taacaacgaa atctgcggat
     1201 ttataagcgc tccttgcatc caaagtagca gttaagtcaa gttctttttc tgtaagatac
     1261 ttttcaatat attcatcctg aattggagac ttccgtttat taatcagttc aatcttttca
     1321 gggataatgt ctaccgcggt tacctggtga tgctgcgcca atagtgtggc gatactcaaa
     1381 ccaacataac cggtgccggc tactgcgatg ttgtactttt tcttcacaat atctcctctt
     1441 tcctctctta agtatggaga atgttactcc tacggctatt catcatgtca aaataacaca
     1501 gcttagaatg ttgataaaat aattttccag aatgtgataa aatctctttc atttataaac
     1561 cacacatcta attttatcta ttctccacaa gacatgtaga gtattcttta ataacaagat
     1621 acgaccttac taaatacttc aaaaaattat catatttaat tcataaatat aaatcttcat
     1681 tttaaatgta tgcccaaaaa tttatctgta atgcgtcatc aaaatctaga ttccaaatct
     1741 ttatcaattt ttatccactc attctaccac tttagtaata ttttctattt ataacacttt
     1801 cgtcctacat tgatcccccc ctctgaagta cagtttttac tgtcttaaat aaaattttaa
     1861 aatctaaccc catactccaa ttttttatat attccgtgtc aagttttgct acttcttcaa
     1921 aatcagtaat atcacttcta ccacttacct gccacattcc agtaattcct ggcttaatag
     1981 caattcttgc acgatgatga taatcatatt cttcatactc tgatttaagg catggtcgaa
     2041 atcctataag gctcatcttt cctgttagaa cgttgaaaaa ttgagggaat tcatctaaag
     2101 acgtggatct aataaattga ccgattcctg tcttttttgt tccatctgga agtattttat
     2161 ttccgataat tcttggatca aactccatct taaacataag attattgctc atcttatttt
     2221 ctttcattaa ttctactttt ctttcttctg catccatata catacttcta aacttataca
     2281 tcttgaacgg tttaccattc tgacctatac gttcttgtgc aaagaaaatt gggcctggtg
     2341 aattaatata aataagtgga gcaataaaga taaaaataat tcccgttgca atacatccaa
     2401 caatacctgc aagaatatca attgttcgtt ttataaatgc ctgttgagtt gtcataacat
     2461 tgatacttgt tgtaagtact gtatattctc ctatacgacc aatagattgt tttcccaaag
     2521 cactatctga tacttttgca agattcaaat gtacagtaag gcccatttca gagcatcgtt
     2581 caataaattc tttcatacac gaagtacttt tattcaaaat aataaagact tcatctaccc
     2641 atccctgaca taagtaatcc gctgcatttt tggaatcaga aacaactgtc actccctcaa
     2701 catcttcacc aatcatgctt ttatctatga taatgacacc atttatttta tacattttat
     2761 agttatgctc tttcacatta cgaataacta cttcagcaat actactagat gtaactataa
     2821 taagtgattt gttcccctca tttttcattt tatgtttcaa gtaatgtttc cataagaggc
     2881 gagttatata tgtaattaca acgtataaac ctcccatcaa gaaaagaacg actctagaat
     2941 aatcatttcc accctgaaca ctaatgagat acaaacttcc taacagcacc aacataatcg
     3001 cttgctttac agaagaagca aattctatat agtaccctct ttttaataca ccctatatgc
     3061 ttcaaacatg aaaataacta aaagatctgc cagttcaatg aagactgcca tgttttgata
     3121 cattggaatc acataaggat tccaatctac ctggcgtaag acatacgata gataaaaagc
     3181 aagctggata cacaataaat ctaaaataat aaaatcaata tgtttaaccc atccccttga
     3241 acttttcttg tacattcctt tttactcccc tctttttaat aaaataaatt gtattacttg
     3301 ttttctctcc taaatatctt tctgaaggat tattgactcg acaaattggc ttgtggatat
     3361 tcttatatat agaacatccg taagccaact taaaactcct taacaaaatt tttttactca
     3421 tattgtagat caaaacaagt atccaagaaa tcatatttta tttctttttt ctgcttactc
     3481 aacaacagct aaaagaaaaa tcatcataaa caaattcaac taagccaacg atccccttgt
     3541 gaaatcaagt tttttcttga tttacgaata aagagctaaa ataaaccaaa cttcttcttt
     3601 tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact
     3661 tgtttcatct gtactacttt ttccttacca aagtcctttg caatctgctc atagcactct
     3721 tttaagtaaa aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga
     3781 ttatgtttga ccatcgtttt ggctgttttc tggatgtctt tgccaaatac cccaacataa
     3841 cttggcgcag tgagttgggc caaacatccc atatctagga acggaagcaa atgatttggg
     3901 tcttcgcgaa aatgcacgtt gcgttctgga tggacgatta ctggtgtctt tcctagttct
     3961 aacaactcaa aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc
     4021 agaagatatg tatcatctaa atctgtaaat aatatttcat ctcggtggat gtcttcgatg
     4081 agcgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc
     4141 tctaattcgg cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat
     4201 tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt
     4261 tcagccattg caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata
     4321 tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag
     4381 taataaccag catctttgct atgttctgct ccgttgtaca caacacctaa gactcgtgct
     4441 tgtaccatct ccagcagttc ttttgcttta gtcaaggatt ctttacgtga catattttca
     4501 cgtacgacca ataaagtgcc atccgttttt aaagacatga tctgtgcatc tgttacggct
     4561 actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc ggataggatc
     4621 tgatccattc ttggcgagct taacaattct gatggggtgg gtggcttagg tccactaggc
     4681 aaaatcgaca agttctcgac tggcgttcgt tggatcacat ctgctacact tcctgatgaa
     4741 ctaagtgctg tacttaatcc actggcattg ttcagctgga atgtcttata gacgacgggt
     4801 ttacgtaaat ctgcatcgac tagtagaact cgttgacctg atttagcaaa aacgactgca
     4861 atgtttgctg cagtggtgga tttcccttct ccaggacctg atgaagtcac gacaatcgtt
     4921 ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt
     4981 tcggcgattg gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg agtttgtgct
     5041 gctttttgtt tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc
     5101 gtgatggggc tgttttatcg tttgtagctt ttggtcgtgc aggagctgtt ccttttttac
     5161 ggataccagc gttcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc
     5221 ctaatgtatc tgtcacatat ttttcatctt tcactgtccg atcgagtaat tccaataaga
     5281 atgccagacc tacacctacc atcattccta acattaagcc agtcattaaa ttcaatttat
     5341 tatttggcga aacaggtgtc gtattggcaa ctgcacctga aatgatcgag attttatcaa
     5401 cattcatcac atcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt
     5461 gagcagccac tgcattcgta cttattgcct gaatcgagaa catttgtgaa ttttctgcct
     5521 gattaactga gatggcatct ttgatttgtt cagcagtcat tttcacattg tcttctgttt
     5581 ctaaacgatc tttgacttca ttgagcacta aatcactgac gatcatatct ttatacgtat
     5641 tgatcatttg caggttcgta ttgacatcat tgacatttgc agtgtctgat tgtggcagtg
     5701 tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc
     5761 ctgagatggc caagccagca aacattgaga tgatgattat taccaagtgt ttgcgtaatg
     5821 tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat
     5881 tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct tgatcactga
     5941 ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttaa
     6001 tcgtaccaaa tgcctcccga taatctaaag caattttttt catttcatca aagttcatgt
     6061 ctgttttcat attcgactct agagcattca aaacggattg atagttgctg acaccgttta
     6121 gactcaatac ttttttcgct acgccttcta cgatctttct ttggcgccct tgtctaccgt
     6181 aatcaccatt tgggtcttca taacgcatac gagaataaga taatgcctga tccccgttca
     6241 atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga
     6301 cttcgatacc accaactgca tcaacgagtt cctttagtcc tttcatattg atagagacat
     6361 agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc
     6421 cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg
     6481 tatctcgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca
     6541 tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca
     6601 atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg
     6661 ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg
     6721 ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata atcaaaaata
     6781 tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt
     6841 gcttgaatca acgttttaac tgtcaatagc ttatattgct atagcccatt tgctgagcaa
     6901 cagtttgtgc ttttgttgcg tatgcggctc ttattttttc attccggatc tgctctattt
     6961 gactgagcgc cgtcaaatat tgatcgtaag tagcggtagc tgttacttga ccgtctttca
     7021 gcatcgtatc gaatagctga tctagttgcg tgacttgtgc aatttgggta tcagcgaatc
     7081 ctagatattg cataacgact gttttccaag cactatcctt aaaaaagctc atattttcac
     7141 gtacatgggc gacatctgca cttgccaact tttctttgat gatgacatca tccacagctt
     7201 gctgccagtt tgatacgttt ttagtaaata atttattgac agccgtctgg atcttcagtt
     7261 ttgattctgc atcctctaac tgcgttaaga ctgctttctt ttcttcttgg atcgcagcag
     7321 cttctttagg taattctgat tccttgatgt cataatcatc tgccgaaagt ttgatagaag
     7381 atagtttatg tgacaaattt gaaactgttt cttctgttat ttcaggattt aggaaaattt
     7441 tttcttttgt actgtacagc ttatctactt cttttgataa agaggtcacg gtgtcttttt
     7501 gattggtgat ggcagattct gcgtaattga tccgttgatg atattgccgc tgattattga
     7561 gatggtatcc aaacaagcaa atcacaatag cgcctgtgcc taccgccaca gtagccaata
     7621 acttgacgaa cggcttttcc atagattcac tcctaaatat tgatgataga taagtaaaga
     7681 tgtgattcat tcacatcaga atgagcgctt tttattttaa aagcataatt aaactaatat
     7741 a
//
LOCUS       DASNNU010000110         7735 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.110, whole genome shotgun sequence.
ACCESSION   DASNNU010000110 DASNNU010000000
VERSION     DASNNU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.110"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..2378
                     /locus_tag="U8116_002416"
     CDS             51..2378
                     /locus_tag="U8116_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN8435365.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2504..2833
                     /locus_tag="U8116_002417"
     CDS             2504..2833
                     /locus_tag="U8116_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN8435366.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2873..3877
                     /locus_tag="U8116_002418"
     CDS             2873..3877
                     /locus_tag="U8116_002418"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN8435367.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3874..4320
                     /locus_tag="U8116_002419"
     CDS             3874..4320
                     /locus_tag="U8116_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN8435368.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4470..5285
                     /locus_tag="U8116_002420"
     CDS             4470..5285
                     /locus_tag="U8116_002420"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN8435369.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5491..6663
                     /locus_tag="U8116_002421"
     CDS             5491..6663
                     /locus_tag="U8116_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN8435370.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6825..7319)
                     /locus_tag="U8116_002422"
     CDS             complement(6825..7319)
                     /locus_tag="U8116_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435371.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7316..7510)
                     /locus_tag="U8116_002423"
     CDS             complement(7316..7510)
                     /locus_tag="U8116_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435372.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2604 a         1449 c         1538 g         2144 t
ORIGIN      
        1 ttagaataac gtgtaaagga ttatttgatg attttttatt gagaggaggg atgaaaatga
       61 ttaagaaaac tcaaaataaa atccaacgta tatgtcaaaa acattttgtt tctagactca
      121 aagctgtatc taaaagaaaa tacttacatc ccattatgat ttcgatcatc ttgattttta
      181 ctttcttagg gagcttatca agtatttatg taaacgttta tacagaaacg tcaaattttg
      241 ctaaccaaac gacagaggag acaaatagtc caccctattc tgaaatacag tccatagaaa
      301 gcaccaatca gtctagcaac aacgagacaa cctcaaccac atcctcgact caactgattg
      361 atccgacaga aaaagaaatc acagcagcaa aagcagcagg ggagaaagct tattcagaaa
      421 caggtagaag ccagacattt agtactgttg cggccactgc gaatttcaac ggacatacca
      481 taccagatgg atatacagtg atatatgcca aagatggagt cattgttgcg gttaacggat
      541 cgatgagtgc ggataaaact gcgttacaag gcgtaaccaa agcaatcgct tttattgata
      601 accgtccaga tatccataat ctgtttattg gctccacagt aaatggtact actatcacat
      661 tcccaaaaag tatctatatg ggggtggcta ccgctggaaa tacgatcgat tcagttacac
      721 ccaatgcgga taaaagttat tcttttacac cctcaacatg gggagggctt cctgccggag
      781 gaggtatgag tactttttca atcggtatac agttagcaaa tgatattcca ttcacggggt
      841 actcgatcaa aaatgggcag atcgttgatg gtaagggaaa tgtttcaaat tttccaacct
      901 atcaagcagc aacggttcag gtcaatgcga atcggacagg aactttttct tatacagtca
      961 atggtggagc agcaaaaatc ggaacgtacg ggactacatt atctggaatc catgtgggtg
     1021 acaaagtggt tatcactcca acagcagtaa cgggctattc ttttgcacaa acaaacggtt
     1081 ctttcacagc cctagaaagt aacgtcgata cggtgaccta tacaggcaaa caaacaccaa
     1141 tcactattaa gtatcaagac tattttggac agatgattgc tccttctaaa ataatgaatc
     1201 ttacttacgg aagtgctgca caagacctga cgactaatac gcctactatc aacggatata
     1261 cctttacagc tgtttcagcg atagaaacaa agaaccaatc agcaatgagt gttttagcta
     1321 gtttagatac aaatggaaat gtaatcgtaa aggacgcaaa tggaaaacag atttctgaag
     1381 tcatcctata ttataagaca aatgcaacag tatcaatcaa tgcaaatggc agtaagtact
     1441 atgatggtct ttctgttgta ccgatcgtta aatatgcctt taatgataaa acagaatcaa
     1501 catccctttt aaacaatgat ctcagtattg atcaaatcac atggaatcca actgatttca
     1561 aagcgatgaa tgaaaaggga gttgaagtaa ctgcgcctac tgaaattgga acctatacgt
     1621 cttggcaatt gacaaaaagc ggactagcta aactagctgc cagaacaaac tatttattta
     1681 ctatcgtgca gacatctgat gtctatacga ttaagcaaat cagcggtgcg gtagagctca
     1741 gtggcagtaa gacatatgat ggaaaagcag gtgtcccgtc aatccatgtc aagctagcag
     1801 agggtgtcac tagttcgctt actccagtac aagtggcact tagcagtatg gattacacag
     1861 cagatgcaca atcagcaaaa aatatggtta atgctggcag ctatacaatt aaactaacga
     1921 atagcggaat agacaaagtc aaagcagcaa atcccaagtt gtcatttact gatatagcca
     1981 acacatcagg aacttatacc atctataaag ctaacgcagt gattactgtc gatgacgctg
     2041 cctttaatta tgatgcccaa agtcactcga tttcggttgg aaatctccac gttaccggtg
     2101 ttgtcttagg cgaatcattg gattataccc taacagttaa cagccggaca gatattggat
     2161 cacaaatagt aacgattagt ttaggcgaag gaagtgtaaa caataattat acgattatcg
     2221 caacagatac cgctaagttg acgatcaatc cgactctctt gcttccttcg acaggtggga
     2281 taggtctact gccctttgtc tttttaggac tgatctttat tttttctggc ttcttttatt
     2341 ttatccatcg taagaaaaaa gcaggtgaac agcgatgaaa aaaacaaaaa atgtacgtgt
     2401 ggatatattt gctgttctct tttttatcct actggtcttt tttataggtt ctccgactgt
     2461 gtcagctaga gaatcaccta ttacatttac tttgcataat cgaatgttcg aaagcgataa
     2521 gatgccaagt atcagtcaaa acgacggaat aaatatcccg gataactttc aaggagtaaa
     2581 agggttgaac ggcgttactt tcgctatata tgatgtctcg gatgaatttt acaagctgcg
     2641 gtcagaagga agtagtgtgg aagacgcaca aagaaaacta gcacaaaaaa gtgatagtga
     2701 aaaaatactg gcagaaaatg tgacaaaaac agtagacgag gaagagggca ttgcttcttt
     2761 tagtgcatcg gacaaagatg agcaaggtcg tgacgctgtt taccgattta cggaaataaa
     2821 gacttcagat taagtaaaag aaaaatcagc accatttgtc gttgtgcttc ctgtgacaga
     2881 ttcatctaat cataaattga cgaccatcca cttgtatcca aagagcgaac aaaaaatacc
     2941 tgctgcactc acattaacaa aaacagtaga gaataagcag acagattttg cagatgggga
     3001 taaagtccct tatctcatca ccgtaacaat tcctgagaac atcaacgaga taggaaccta
     3061 tacgatcaaa gatacagctg atcctcagtt atggctagaa ccagaaacga tcgatttttt
     3121 gattggtgga gacaagattc atacatttca cacacaaaaa ataaaacatg gttttgtctt
     3181 atcagattct ggaaaagatc ttagttcgtt tgctggaaaa aaattaacga tccgttacca
     3241 aatgacattg aaagaaaaca gtgaaaaaac gacatttgat aacgatgtac gtttgacgac
     3301 tgacaatcaa acagtagaaa cacatcttgc catccaaaca ggaggcaaac aatttgtcaa
     3361 agtggatcgg caaacgaaac aaaaactagc agatgctcaa tttctagtca aaaaaaatga
     3421 ccaatactta gcgcaagaaa acgggataaa tcattgggta gccaaagatg attcaagagc
     3481 aacgattttt acttccagta aagatggaac attttctgtc catggattgt cttttggaag
     3541 ttacgaacta gtggagatta aaccgcctaa aggatatata ttgagtcaat caaccattct
     3601 ctttgaagtt gaaaaaggca gttatgatcc gcttcatcct ataccattgg aaatcatcaa
     3661 cgaaccaaaa gcaaatccga cgaataatgg aaaaacgcca gttcagatgc agacaaatgg
     3721 tccatcaaat gaaaaaaatg gaggaagctt tccaaaaaca agcgaagaga tgcttggagg
     3781 tttctcaatt ttagggttat tgctagtcct ttcaacaggg acagcttggt tttataaaaa
     3841 acaacaaaaa ggaaacaata ggagggaaat aaaatgaaaa acatcaaaac aaaaatatgg
     3901 acgtttttag gaactgctat catgctgctt cctttcgttc tcggcctagg aactgcagag
     3961 gtttctgcag ccgtatcacc tacaccagaa aatgttacgg ttaatttgca taaattgaaa
     4021 ttcacttcag caccagaaaa tcaaataaac aacggaacgg aattgacatt tccgaatagc
     4081 gaaccgttga acggcgttga attcaacgta tatgacatca ctgctacgta ctatccaagt
     4141 aaagacacag cagttccagc agacgctaca cccttcgcaa gtgtaacgac ttctggtgaa
     4201 ggattagcta acctgactct tccaggtaaa tctgatggga aagatgctgt atacgtcttt
     4261 gtagaaacac caaagcccgg tgtcgaaacg tcgccaaata tcgtgttaag cttaccctag
     4321 accctgacaa acctttagac acgattcacc tatatgctaa aaatgtcatc aaagaaagca
     4381 atatcaaaat ccagaaagct gggaactttt caggggtaga taagttagca ggggcgcaat
     4441 tccagatcaa aaattcagag aatcaattcg tgaccggttt tgatgaaaac actggtgtcg
     4501 ctctctatga tcctgctgag tcagcgttga cattcacaac tgatactgaa ggatccatca
     4561 gtgtcaatgg attaatagac ggcaattata ctttagttga aacaaaagca ccagatggtt
     4621 atcaattaac caatccacaa aaagaaacta cttttatagt aaaaaacgga aaattgcaac
     4681 aaacaaccgg tatcatccat ggcgcagatg aagccaatct tgggaatcca aacggtgtag
     4741 tcaatcggga aaatacaact acggataaca cgattactgt agaaaatgtc caaaactacg
     4801 gtgatttcca cttcatcaaa caagatgtga acacgcaaga aaaattagcc gatgcagaat
     4861 tttctattct aagagcaaaa gatccaactg ttaaattaat gaaaaaaata gcaaaccttg
     4921 cctcaggaga atacccatat gcttggtcag atgaagtcac aggttctgac tgggaagaag
     4981 tgaaattgat ttctgattca gaagggaaat tttcaatcga agggctgaaa caaggggcat
     5041 actgccttca agaaacaaaa gcaccagaag gctacgcatt acctaagaat gaagctgcgt
     5101 acactgaatt tacagtagat gatgatccaa caacttctga tgaaacaacg atcgataacc
     5161 aacctaaagg aatcttgcct tcaactggtg gtatggggat catcgtattc atcttggtag
     5221 gtactgcttt agttggagga gcagtgatct atttcaaaaa acgccatcaa gaaacagaag
     5281 cttaaactat tggaaacaaa aatcatacga taaataaacg atagtgattc aaatggggag
     5341 aaaggttgtg acaagtggtc ggtctcttgg tattcgctca acaaacggca aaataggagg
     5401 aactattttg ccgtttgctt cgctaatact caggtcaagg cgctgatttc acaacctctt
     5461 catatacata ttagaaaata ggtggacatg atgaataaaa gcagaataag aatactggac
     5521 atcttcatgg caatcatttt agtcgttggg atcgggatct tttcttatcc ttttgtagaa
     5581 gattcactga atgattttct tgcccagcaa atgatcatcc attatcaaaa gcaagcatcg
     5641 aataagaatt cagaggaaat aaaaaaacag caggaaaaaa tgacaaagaa aaaccaacag
     5701 ttggcagagc aaaacgtatc accagggata ggcagtttca accaagcagt tgatgcaaaa
     5761 gcattaaaag atttacctag taatgccttt tttatggaac atatgcttgg agtcatcgaa
     5821 atcccaaaaa tcaatgtcag cttacctata ttcgatcaga caacggaaat atttttgcaa
     5881 aaaggaacgt cattattgga agaaagttct tatccgactg gcgggaaaaa cacccatgcc
     5941 gttctttctg ggcatcgtgg actcccagaa gcaaaattat ttacagattt gcctaagttg
     6001 aaaaaaggag accagttttt catccagatc aatggaaaga cattggcgta tcaagtagaa
     6061 aaaatccaag tggtcttacc tgatgaagta gattctttgg gtatccaaaa aggcagagat
     6121 ctcgtgactt tgcttacctg tactccttat atggtcaata cacatcgact gcttgtgacg
     6181 gggcatcgta ttccttatca agcaaaagag gcaaaaaaag cgatccaagg aattgatcaa
     6241 tggaaaaaat ggaaattttt tatttggttt atcggtatac tacttggatc aatcggctta
     6301 gtttggctac tcatcgctta tttagattct ttggccatcg ctaagcgaaa ctatccttta
     6361 tctttttatg taaagaacac aaacggacga ccaatcgaag gaatggtctt ttctgtaaaa
     6421 acgctcaacg gcaaacacta tattactaga gaaaaagttc catttgtgaa agcttctgat
     6481 gaatacggac tagtgagatt ttctgattta aaaggcagga actatcgctt gcaacatgag
     6541 gagctcctgc ttaaaatcca tgtaaaacat aaacattcga aacagttttc aatgaaattg
     6601 aaaaaaggaa gatataaact gcgaaaagaa aaagaggtgt actatttgat tgagaaagaa
     6661 tgaatgaaga tgtccaaaaa agcagaggga acagactaaa tgaaaaaaga gagaagctga
     6721 cgaaatggac gtgtaaactg ttatttttgg tttactatct ggaacgccat cttctatttt
     6781 tttatgtttc agaagaattt tattattata gtataacttt tgaattagtt tccataacat
     6841 ttggagcaag gggttaaacc acgagcttga gcttcgctta aagtggcagg ggtatacgta
     6901 ccatttccac atttatgtgt atggtatttt gaaccggtcg gtgtgacata gacagtttgt
     6961 tcattatttt gctcaggagc tgcggcttgt gcctgctgag aggcctgttc agctgctgat
     7021 tgctgtcgcg cctgttctgc ttgttgctgt gcgattgctt cttgcctcgc ctgttctgct
     7081 tgttgctgtg cgattgcttc ttgttgcgct tgttctgctt gttgtcgtgc aatttcttct
     7141 tgctttttcc gttcttcttc tgcttctttt gctttgattg tggaatcaat tgcagcaaga
     7201 cggcttgtca actcttgttt gttttcaggt agcgcttgga tagctgctga agcagttgca
     7261 tagttttctt ttgtaggatt tgcttcagct tgtgacaaag cagattccgc ttctgtcact
     7321 ttttttctag tgcagctttt tccgcttttt gtttctcttc ttttttcttt tcaatttgtt
     7381 cttgttcttt cgatgaatct gccgtacttt cggagatcgt ggtactgctg ctggaagcct
     7441 gatctgaact tgtgctttga gtagtagacg tagtagcacc acacccactc aacaaacttc
     7501 ctgcgaccaa aaacaatgca actaattttt tcgctaaatt tcctccttaa gtaaaaaaca
     7561 accgttttca ataaatgtcc ttttcaattg tttacttact tgttataata attgtgttat
     7621 gaatagtgat tgaggaaact taatctaatc ccgtgttgat acacgggatt ttttgctata
     7681 gttctgttat taagtatgca ataataaaat atgtacgtta ttttattatt agatt
//
LOCUS       DASNNU010000111         7614 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.112, whole genome shotgun sequence.
ACCESSION   DASNNU010000111 DASNNU010000000
VERSION     DASNNU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.112"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..568
                     /locus_tag="U8116_002424"
     CDS             <1..568
                     /locus_tag="U8116_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN8435373.1"
                     /translation="QNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAVKKTVFYGR
                     NGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRDMVDYWNDC
                     FNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIREISPIDLTE
                     LMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            698..1345
                     /locus_tag="U8116_002425"
     CDS             698..1345
                     /locus_tag="U8116_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HEN8435374.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(2038..2976)
                     /locus_tag="U8116_002426"
                     /pseudo
     CDS             complement(2038..2976)
                     /locus_tag="U8116_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(2976..3593)
                     /locus_tag="U8116_002427"
     CDS             complement(2976..3593)
                     /locus_tag="U8116_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN8435375.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(3607..3777)
                     /locus_tag="U8116_002428"
     CDS             complement(3607..3777)
                     /locus_tag="U8116_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435376.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(4125..5615)
                     /locus_tag="U8116_002429"
     CDS             complement(4125..5615)
                     /locus_tag="U8116_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN8435377.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(5658..5957)
                     /locus_tag="U8116_002430"
     CDS             complement(5658..5957)
                     /locus_tag="U8116_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435378.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(6323..6607)
                     /locus_tag="U8116_002431"
     CDS             complement(6323..6607)
                     /locus_tag="U8116_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8435379.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(7126..7611)
                     /gene="vanZ"
                     /locus_tag="U8116_002432"
     CDS             complement(7126..7611)
                     /gene="vanZ"
                     /locus_tag="U8116_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEN8435380.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT         2391 a         1274 c         1175 g         2774 t
ORIGIN      
        1 acaaaatatt atcgactaca tggaagatga cggttttaca agattagact tagcttttga
       61 ttttgaagat gatttgagcg attactatgc aatgactgat aaagcagtta agaaaactgt
      121 tttttatggt cgtaatggca agccagaaac aaaatatttt ggtgtccgtg atagtgatag
      181 atttattaga atttataata aaaaacaaga acgtaaagat aacgcagatg ttgaagttgt
      241 gtctgaacat ttatggcgtg tagaagttga attaaaaaga gatatggttg attactggaa
      301 tgattgtttt aatgatttac acatcttgaa acctgcgtgg gctactttag aaaaaattaa
      361 tgagcaagct atggtttata ctttgttgca tgaagaaagt atgtggggaa agctaagtaa
      421 gaatactaag actaaattta aaaaattgat tagagaaata tctccaattg atttaacgga
      481 attaatgaaa tcgactttaa aagcgaacga aaaacaattg caaaagcaga ttgatttttg
      541 gcaacgtgaa tttaggtttt ggaagtaaaa taagttttat ttgataaaaa ttgctaattc
      601 agtataatta atatttacga ggtgacataa cgtatgaaaa aatcagagga ttattcctcc
      661 taaatataaa aatttaaaat ttaggaggaa gttatatatg acttttaata ttattgaatt
      721 agaaaattgg gatagaaaag aatattttga acactatttt aatcagcaaa ctacttatag
      781 cattactaaa gaaattgata ttactttgtt taaagatatg ataaaaaaga aaggatatga
      841 aatttatccc tctttaattt atgcaattat ggaagttgta aataaaaata aagtgtttag
      901 aacaggaatt aatagtgaga ataaattagg ttattgggat aagttaaatc ctttgtatac
      961 agtttttaat aagcaaactg aaaaatttac taacatttgg actgaatctg ataaaaactt
     1021 catttctttt tataataatt ataaaaatga cttgcttgaa tataaagata aagaagaaat
     1081 gtttcctaaa aaaccgatac ctgaaaacac cataccgatt tcaatgattc cttggattga
     1141 ttttagttca tttaatttaa atattggtaa caatagcagc tttttattgc ctattattac
     1201 gataggtaaa ttttatagtg agaataataa aatttatata ccagttgctc tgcaacttca
     1261 tcattctgta tgtgatggtt accatgcttc actatttatg aatgaatttc aagatataat
     1321 tcatagggta gatgattgga tttagttttt agattttgaa agtgaattta attttataca
     1381 cgtaagtgat cataaaattt atgaacgtat aacaaccaca ttttttggtt gcttgtggtt
     1441 ttgattttga atttggtttt gaacttatgg actgatttat tcagtccatt ttttgtgctt
     1501 gcacaaaaac tagcctcgca gagcacacgc attaatgact tatgaaacgt agtaaataag
     1561 tctagtgtgt tatactttac ttggaagatg caccgaataa aaaatattga agaacaacta
     1621 gcaaaagatt ttaaagagtt attttatttt aagtctttat aacatgagtg aagcgaattt
     1681 ttaaatttcg atagaaattt ttacatcaaa aagccccctg tcaaaattga cgaagggggt
     1741 tttttggcgc acgcttttcg ttagaaatat acaagattga aaatcgtgta taagtgcgcc
     1801 ctttgttttg aacttagcac gttacatcaa ttttttaaaa tgatgtataa gtgcgccctt
     1861 ttaaattttg agtgattata ttttttgagt tagaaaaagg gattgggaaa atttcccaaa
     1921 ataatttaaa aaataagcaa aaattttcga tagagaatgt gctatttttt gtcaaaggtg
     1981 tataccttga ctgtgcttgc tgttacatta agtttatttt taagttatta aaaaagaaat
     2041 agcttttaaa gtttggctcg ctgtcgcttt ataaagctga ttgacttttg attgcaaact
     2101 acttaaagaa aacaaactcg gactattcgt tttcttctct ttggtttgaa catcagcaat
     2161 tatcccctct tgattgccta ttttagcttg tttagaagaa acaaaagcta aaagctcctc
     2221 ttgggtttta aaacgctgtg tggggcttag aacgccctta aacgaccctt ggtttacttt
     2281 tatactagct tccacctcga aaaaaggttc ttttttaaaa ttctctatgg cttcctggcg
     2341 ctgaaaaata aggtataagg tgggcgtttg aacacgtcct agtgaaaatg taccttgtac
     2401 gcccttctgt tgtaaattta acgtatacaa agggcttgcg ttcatgccga tcaaccaatc
     2461 ggcaatttgg cgtgtttgcg cttcttgata aaagggatag taattcattc caggttgcaa
     2521 attttgaaaa ccgcttcgga ttacatcttt ttctaagcta ttgatccata gtcttttaaa
     2581 tgttttatct tttgaaaagg catttgcttt atggataatc gaccaggcga tattttcacc
     2641 ttctctgtcg ctatctgttg caacaataat tgtatttgcc tttttgagaa gttctgcaac
     2701 aattttaaac tgctttccct tatcttttgc aacttcaaaa tcgtatcgat caggaaaaat
     2761 cggcaaagat tcaagtttcc aattttgcca cttttcgtca taatgacctg gttctgctaa
     2821 ttccactaaa tgcccaaaac caaaggtgat aaacgtttca tctgtaaata gtgggtcttt
     2881 gatctcaaaa taaccgtctt ttttggtgct ttgttttaaa gcacttgcgt aggctaatgc
     2941 ctggcttggt ttttcagcta aaataaccgt actcattaac tatccctctt ttcattgttt
     3001 tttctttgat cgactgtcac gttatatctt gctcgatacc ttctaaacgt tcggcgattg
     3061 attccagttt gttcttcaac ttctttatcg gataaaccat tcaaaaacaa atcgaaagca
     3121 tgctttaaac gtggatcatt ttctttaaat ttatgctgac gacctttgaa tttgcctttt
     3181 ttcttagcaa tttcgattcc ttgtgcctga cgttccttaa ttttttttcg ttctgattct
     3241 gcttgatact tgtacaattc aatgacaagg ctattaatca aacgccttaa attttcatct
     3301 tcaataccat tcattgaggg taaatttaag acttccaggg ttgccccctt aatttgaatt
     3361 tgattcatca attctgttaa ttctttatta tttcgtccta atcgatctaa ttcagtaaca
     3421 ataacaatat ccccttcacg aatatagtta agcatagctt gtaattgtgg gcgttcgacc
     3481 gattgaccgc ttaatttgtc tgaaaagacc ttagaaacgc cctgtaacgc ttgtaattgc
     3541 cgatctaagt tctgttcttt gctactgaca cgtgcataac caattttagc cattttcaac
     3601 caacctctaa aattctctcg gttgcaataa ccaatcagca atatctactt tttcaatttc
     3661 aaattgctta tcagaaattg tcttttcgta agcgataaaa tcttgcgcat attgttgctc
     3721 attaaaaata gccaccactt cgtcattttc taaaactcga taaataaatt ttttcatttt
     3781 actcctccta ttatgcccaa cttaaatgac ctattcacca agtcaattat actgctaaaa
     3841 tcatattagg acaaataggt atactctaat gacctataaa agatagaaaa ttaaaaaatc
     3901 aagtgttcgc ttcgctctca ctgcccctcg acgttttagt agcctttccc tcacttcgtt
     3961 cagtccaagc caactaaaag ttttcgggct actctctcct tctcccccta ataattaatt
     4021 aaaatcttac tctgtatatt tctgctaatc attcgctaaa cagcaaagaa aaaacaaaca
     4081 cgtatcatag atataaatgt aatggcatag tgcgggtttt attttcagcc tgtatcatag
     4141 ctaaacaaat cgagttgtgt gtccgtttta gggcgttctg ctagcttgtt taaagtctct
     4201 tgaatgaatg tatgctctaa gtcaaaagaa tttgtcagcg cctttatata gctttctttt
     4261 tcttcttttt ttactttaat gatcgatagc aacaatgatt taacactagc aagttgaatg
     4321 ccaccatttc ttcctggttt aatcttaaag aaaatttcct gattcgcctt cagtaccttc
     4381 agcaatttat ctaatgtccg ttcaggaatg cctagcactt ctctaatctc ttttttggtc
     4441 gtcactaaat aaggcttgta tacatcgctt ttttcgctaa tataagccat taaatcttct
     4501 ttccattctg acaaatgaac acgttgacgt tcgcttcttt ttttcttgaa tttaaaccac
     4561 ccttgacgga caaataaatc tttactggtt aaatcacttg atacccaagc tttgcaaaga
     4621 atggtaatgt attccctatt agccccttga tagttttctg aataggcact tctaacaatt
     4681 ttgattactt ctttttcttc taagggttga tctaatcgat tattaaactc aaacatatta
     4741 tattcgcacg tttcgattga atagcctgaa ctaaagtagg ctaaagagag ggtaaacatg
     4801 acgttattac gccctattaa acccttttct cctgaaaatt tcgtttcgtg caataagaga
     4861 ttaaaccagg gttcatctac ttgttttttg ccttctgtac cgcttaaaac cgttagactt
     4921 gaacgagtaa agcccttatt atctgtttgt ttgaaagacc aatcttgcca ttctttgaaa
     4981 gaataacggt aattaggatc aaaaaattct acattgtccg ttcttggtat gcgagcaata
     5041 ccaaaatgat tacacgttag atcaactggc aaagactttc caaaatattc tcggatattt
     5101 tgcgaaatta ttttggctgc tttgacagat ttaaattctg attttgaagt cacatagact
     5161 ggcgtttcta aaacaaaata tgcttgataa cctttatcag atttgataat catagtaggc
     5221 ataaaaccta aatcaatagc ggttgttaaa atatcgcttg ctgaaatagt ttcttttgcc
     5281 gtgtgaatat caaaatcaat aaagaaggta ttgatttgtc ttaaattgtt ttcagaatgt
     5341 cctttcgtgt atgaacggtt ttcgtctgca tacgttccat aacgataaac gttgggtgtc
     5401 caatgtgtaa atgtatcttg attttcttga atcgcttcct cggaagtcag aacaacacca
     5461 cgaccgccaa tcatgcttga ttttgagcga tacgcaaaaa tagccccttt gcttttacct
     5521 ggcttggtag tgattgagcg aattttacta tttttaaatt tgtactttaa caagccgtca
     5581 tgaagcacag tttctacaac aaaagggata ttcattcagc tgttctcctt tcctataaat
     5641 cctataaaat aggttgttta attaacttgg tttgcttttt cattcaactg tttcaatatt
     5701 gcatgttttg aaaaagattt ttttccttta taagtcaatt tttttccact aatcgaataa
     5761 attattttgt tattttctat taacttatat atataatctt ccccctccga agaaaaatac
     5821 ttatctgatt ttgtttctaa gtagatattt ctcttttcta actctttctt aaacgtttct
     5881 agtgtataga tatttgctaa ttttcttatc tccaataaac tattttttat ataagtttta
     5941 cattcatcat gattcataca aactccacct tctataaatg aatacaaaaa aagcaatcaa
     6001 acgatttccg attgattgct taacaattct taaattcagt agcttagata cttgaaaact
     6061 ctctgatttc cctatataat gatagtacgg ttatataccg tcttcaaaca aagttaatta
     6121 aataacttct tacgagggaa gagttcatct gactaactga taagcgttgg tttggcaatc
     6181 ttatcgggct atgcatttat aaaatgtcgt caaacatttt ataaatgtgt catggctctt
     6241 ttttcgtttc tattcagttc gttgtttcgt tatatctagt ataccgcttt taaaaaaaat
     6301 aagcaacgat ttcgtgcatt attcacacga agtcattgct tttttcttct tccatttcta
     6361 aatccaatgt tacttgttct gattctgttt ctggttctgg ttctgttggc tcatttggga
     6421 ttaaatccac tactagcgtt gagttagtta actttgcaat ttgttctagt gtttttatgg
     6481 ttggatctga ttttcctgat tctattcgtg aataatttga tctactcatt tctaattctt
     6541 gggagaattt ttcttttgat ttggtacctc tcatcttttt taagctttct ctaaatgcta
     6601 gttccaagtt tctcccccct ctttttgtgt aataaaattc acgctttatt taaattatag
     6661 cttttaatat cttaataaac aagtgattaa tcacttgtga tttgtgatta ttccaaaagt
     6721 aggtaatttc accaaccttt ttcaaaagtt tttatcgacg attattggac tggatcgtgt
     6781 ttcctaaagc gattctaagc gcatgtttaa ttttaaagtg ggtaattctc tttaaaacgt
     6841 cctacaaaga ttcaaaaaaa gcatggctgc tagtgggggt accgtcagga aaatgcggat
     6901 ttacaacgtt aaggaaattt attaaagaga ccaattcctc agcactaaaa ttgataattg
     6961 ggctttcttt gttactatag tgttaggtaa gctttttact ttatcaccgg ttttcccctc
     7021 acttcacacc tacgggcgag tttccccaca tcccgttttc catctaatcc aattcattca
     7081 atatctttat gtgtatgaaa gatcagattg cttgatttag acatcttaca cgtaatttat
     7141 tcttaaatgg gtacggtaaa cgagcaataa tacgagcaaa agtataccta caaaaataat
     7201 aactctgtct aatttttttt gattcatatg cttattgctt aaaccatata atttcagtcc
     7261 aagaaagcct ccaacagtat ttgtaattac atctgttatg tctgtcgctc caatagcgaa
     7321 gataaattga attatttcaa aagtaagact taaaaccagt acaaaagcaa acttaggtaa
     7381 aaatccgatt tctttaaaat tgacattcaa aagcaagcca aatggaataa agattataac
     7441 attatctatc atctctctga aattcccagt agcagtaaat ggagtcaagt taagacttct
     7501 ttgatgataa ttaaatactg ataaaatatt gtattgtaat ttgaataaca ctaaccagat
     7561 tagtgtcact aaatataaag ctagcaatcc tctagataat atttttccca atat
//
LOCUS       DASNNU010000112         7217 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.113, whole genome shotgun sequence.
ACCESSION   DASNNU010000112 DASNNU010000000
VERSION     DASNNU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.113"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="U8116_002433"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="U8116_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEN8435381.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /locus_tag="U8116_002434"
     CDS             complement(919..1677)
                     /locus_tag="U8116_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN8435382.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="U8116_002435"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="U8116_002435"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN8435383.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="U8116_002436"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="U8116_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEN8435384.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="U8116_002437"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="U8116_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEN8435385.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="U8116_002438"
     CDS             complement(4433..5290)
                     /locus_tag="U8116_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEN8435386.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="U8116_002439"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="U8116_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN8435387.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttat
//
LOCUS       DASNNU010000113         6745 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.114, whole genome shotgun sequence.
ACCESSION   DASNNU010000113 DASNNU010000000
VERSION     DASNNU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.114"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            36..926
                     /locus_tag="U8116_002440"
     CDS             36..926
                     /locus_tag="U8116_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEN8435388.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(999..1823)
                     /gene="nadE"
                     /locus_tag="U8116_002441"
     CDS             complement(999..1823)
                     /gene="nadE"
                     /locus_tag="U8116_002441"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEN8435389.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1835..3307)
                     /locus_tag="U8116_002442"
     CDS             complement(1835..3307)
                     /locus_tag="U8116_002442"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEN8435390.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3944..4306)
                     /locus_tag="U8116_002443"
     CDS             complement(3944..4306)
                     /locus_tag="U8116_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN8435391.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4529..5281
                     /locus_tag="U8116_002444"
     CDS             4529..5281
                     /locus_tag="U8116_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN8435392.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5421..6344)
                     /locus_tag="U8116_002445"
     CDS             complement(5421..6344)
                     /locus_tag="U8116_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN8435393.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6359..6631)
                     /locus_tag="U8116_002446"
     CDS             complement(6359..6631)
                     /locus_tag="U8116_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435394.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2060 a         1349 c         1155 g         2181 t
ORIGIN      
        1 aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata aacgaagaat
       61 ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc cagctacttt
      121 ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc agtgctatca
      181 cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa aaagtaggac
      241 aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca gaaaaaatat
      301 tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca aattatgcaa
      361 ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg gatgaaccac
      421 gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc agcggatttg
      481 tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg ttggaagttt
      541 ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca gatatcactt
      601 tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt gctgatatcc
      661 gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc ttgctgaaga
      721 cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca aacatcacga
      781 tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg aaagacgatg
      841 ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac gatcaggata
      901 tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa acaaaaacag
      961 ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa atcatccaaa
     1021 acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc gattttttct
     1081 tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt atatgtgaca
     1141 cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc agtcggaatt
     1201 tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc ttgtcgtttg
     1261 tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt gaagaatcca
     1321 gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc tccagctacg
     1381 gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa atcgctgatt
     1441 cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt gatatttact
     1501 cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc ttcatctgct
     1561 tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt tgtttcttca
     1621 cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc ttggccgccg
     1681 ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc tttcataaag
     1741 tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc taattcattg
     1801 ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga agttctgcta
     1861 cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca gtagacagat
     1921 cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt gaatctagat
     1981 tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact ctttttcctt
     2041 cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt ttattgataa
     2101 acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct tcatcccata
     2161 atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc cacacttgtt
     2221 ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta tccatcattt
     2281 ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct ggctgatcat
     2341 aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt tgcatcttta
     2401 aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct tccgtaaatc
     2461 cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct ccactgtcga
     2521 tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga atcgcacttg
     2581 gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct ttatgggttt
     2641 tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac gaatgagcat
     2701 gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt gttgcatctg
     2761 ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct tgtgcccgtc
     2821 tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga gctgcttttg
     2881 tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact aattggcagt
     2941 gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt tctccttcac
     3001 gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa aaaccttcag
     3061 gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc agattctcta
     3121 aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta aaaggcattt
     3181 ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc cagtatgtct
     3241 gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct gggtaagtat
     3301 tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta taacaaaaaa
     3361 aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt acggatattt
     3421 cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat ttaacctaaa
     3481 cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta gcgttttcta
     3541 tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg ttaagatacc
     3601 gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa aagtttttga
     3661 agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat ataacttatt
     3721 atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg ttgaaaaaac
     3781 tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca gctgtcaata
     3841 gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta ttgtatacat
     3901 tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata gaatccttcg
     3961 tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt attccggcac
     4021 gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc atttttaaaa
     4081 cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta ttgatcatca
     4141 cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct aacgaaaata
     4201 atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta ggataaaatg
     4261 tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca ttcctctttt
     4321 ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta gcattaagga
     4381 atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact taatagtata
     4441 acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac gtaaaggatt
     4501 atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa tgtcagagat
     4561 atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc taaaaccagc
     4621 taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc gtctgcaaca
     4681 catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg tcttagacaa
     4741 tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc tggaaaaacg
     4801 ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt acgaaggagg
     4861 aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt tgaaagaaaa
     4921 tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg ttacagcatc
     4981 caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa ctgttgaaaa
     5041 gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa gcttagcaga
     5101 ccgtatcttc caagctcata agaagtggtt atcttacagc tggcctgaat atcagccaaa
     5161 agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg catattatga
     5221 cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa aaaaggcgta
     5281 atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca aaacagcaga
     5341 cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc acaagttttt
     5401 tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt cactttatcc
     5461 tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag atggtggtcg
     5521 atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat caccactggt
     5581 gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga atttgtaaag
     5641 acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt ctctgcatct
     5701 atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg acatagctgc
     5761 aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat gcccaattgc
     5821 tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc tcgatcaata
     5881 aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg tccatatttt
     5941 tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa tccatcgaaa
     6001 gctttagctg caaattcttc aagatacctt cgctcttttt ctttctgata gcccgtcggt
     6061 aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact gatcaactga
     6121 tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt ttttccataa
     6181 cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac cgcaagtatt
     6241 tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga aatagtagaa
     6301 actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc ttcgccacct
     6361 atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca aaaaactgat
     6421 acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata tcttttgtca
     6481 aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac ccttgaaaac
     6541 tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa ttgttcgttg
     6601 tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat actttgagta
     6661 tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa gttttattcc
     6721 tctttcatta ttaccaaagt ctctt
//
LOCUS       DASNNU010000114         6713 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.115, whole genome shotgun sequence.
ACCESSION   DASNNU010000114 DASNNU010000000
VERSION     DASNNU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.115"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(105..629)
                     /locus_tag="U8116_002447"
     CDS             complement(105..629)
                     /locus_tag="U8116_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8435395.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U8116_002448"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U8116_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEN8435396.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U8116_002449"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U8116_002449"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEN8435397.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="U8116_002450"
     CDS             complement(2351..3031)
                     /locus_tag="U8116_002450"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN8435398.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="U8116_002451"
     CDS             3157..4026
                     /locus_tag="U8116_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN8435399.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="U8116_002452"
     CDS             4052..4567
                     /locus_tag="U8116_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435400.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="U8116_002453"
     CDS             complement(4614..5207)
                     /locus_tag="U8116_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN8435401.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="U8116_002454"
     CDS             complement(5232..5411)
                     /locus_tag="U8116_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435402.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="U8116_002455"
     CDS             complement(5488..5997)
                     /locus_tag="U8116_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435403.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="U8116_002456"
     CDS             complement(6093..6473)
                     /locus_tag="U8116_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN8435404.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         1908 a         1409 c         1179 g         2217 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaa
//
LOCUS       DASNNU010000115         6629 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.116, whole genome shotgun sequence.
ACCESSION   DASNNU010000115 DASNNU010000000
VERSION     DASNNU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.116"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(38..496)
                     /locus_tag="U8116_002457"
     CDS             complement(38..496)
                     /locus_tag="U8116_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN8435405.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(509..1840)
                     /locus_tag="U8116_002458"
     CDS             complement(509..1840)
                     /locus_tag="U8116_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEN8435406.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1851..3293)
                     /locus_tag="U8116_002459"
     CDS             complement(1851..3293)
                     /locus_tag="U8116_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN8435407.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3280..4044)
                     /locus_tag="U8116_002460"
     CDS             complement(3280..4044)
                     /locus_tag="U8116_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEN8435408.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4148..5023)
                     /locus_tag="U8116_002461"
     CDS             complement(4148..5023)
                     /locus_tag="U8116_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN8435409.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5278..5362)
                     /locus_tag="U8116_002462"
     tRNA            complement(5278..5362)
                     /locus_tag="U8116_002462"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5326..5328),aa:Leu,seq:caa)
     gene            complement(5380..5451)
                     /locus_tag="U8116_002463"
     tRNA            complement(5380..5451)
                     /locus_tag="U8116_002463"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5417..5419),aa:Gln,seq:ttg)
     gene            complement(5461..5533)
                     /locus_tag="U8116_002464"
     tRNA            complement(5461..5533)
                     /locus_tag="U8116_002464"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5498..5500),aa:His,seq:gtg)
     gene            complement(5595..5679)
                     /locus_tag="U8116_002465"
     tRNA            complement(5595..5679)
                     /locus_tag="U8116_002465"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5643..5645),aa:Leu,seq:caa)
     gene            complement(5700..5770)
                     /locus_tag="U8116_002466"
     tRNA            complement(5700..5770)
                     /locus_tag="U8116_002466"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5736..5738),aa:Cys,seq:gca)
     gene            complement(5816..5887)
                     /locus_tag="U8116_002467"
     tRNA            complement(5816..5887)
                     /locus_tag="U8116_002467"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5853..5855),aa:Gln,seq:ttg)
     gene            complement(5899..5971)
                     /locus_tag="U8116_002468"
     tRNA            complement(5899..5971)
                     /locus_tag="U8116_002468"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5936..5938),aa:His,seq:gtg)
     gene            complement(5974..6047)
                     /locus_tag="U8116_002469"
     tRNA            complement(5974..6047)
                     /locus_tag="U8116_002469"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6013..6015),aa:Trp,seq:cca)
     gene            complement(6054..6134)
                     /locus_tag="U8116_002470"
     tRNA            complement(6054..6134)
                     /locus_tag="U8116_002470"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6098..6100),aa:Tyr,seq:gta)
     gene            complement(6136..6211)
                     /locus_tag="U8116_002471"
     tRNA            complement(6136..6211)
                     /locus_tag="U8116_002471"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6176..6178),aa:Phe,seq:gaa)
     gene            complement(6247..6319)
                     /locus_tag="U8116_002472"
     tRNA            complement(6247..6319)
                     /locus_tag="U8116_002472"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6284..6286),aa:Asp,seq:gtc)
     gene            complement(6329..6401)
                     /locus_tag="U8116_002473"
     tRNA            complement(6329..6401)
                     /locus_tag="U8116_002473"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6366..6368),aa:Val,seq:tac)
     gene            complement(6417..6488)
                     /locus_tag="U8116_002474"
     tRNA            complement(6417..6488)
                     /locus_tag="U8116_002474"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6453..6455),aa:Glu,seq:ttc)
     gene            complement(6503..6592)
                     /locus_tag="U8116_002475"
     tRNA            complement(6503..6592)
                     /locus_tag="U8116_002475"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6556..6558),aa:Ser,seq:gga)
BASE COUNT         1989 a         1466 c         1081 g         2093 t
ORIGIN      
        1 cataaatatc accctattta acttttttca tcgtacacta aaattcaaaa caactatctt
       61 taatctttac ttcttggttt tgttttaata aatttccttc aaaaatgcct ggagaaacta
      121 caatgatcac cgtatctttt tgtgcttgct ccaacttctt caaatctata atagctaaca
      181 gtgtcccttc ttttacctcc tggttacttt caacccgtat ctgaaacgga cttccttcga
      241 gttcaactgt atctaaaccc atatggatca gtatttttct gccagattca ctttcgatcg
      301 ttatggcatg tcgggtagga aatatattat cgattattcc tttaactggc gcatggatat
      361 atccatcatg attcgttata gcgtatccaa ctcctatcat ttttgaagaa aagacctcgt
      421 cttttactgc tgttaatgga ataatttgac cactagctac agcttttaga atgtttcttg
      481 ttttaaagaa atgcactttt tatcacctct actttaatgc tggccaatat tttttgttta
      541 ctttgatcat ttcatctagt attttttttg cgactctggc atttggtacg gtttgattca
      601 atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa gtaagcatct actagtaact
      661 tctctactgc aacttgtgcc tccatcaatc ctttatgaaa atcactgatt ggttttctta
      721 gtgtaattgg ctccacacca gttgcaccaa catatgccgg tatttcaaca actgcatctg
      781 accgtagatt cggaatcgcc ccttgattag gaacgattag cataaagcga cgacgatcat
      841 cattcaacaa ggaaatagct atatcgacga tatattgtcc atgctcccca aaattaaagt
      901 tcaatacttc ttccacaata ccgcggcgta ttttatcagc catttctttg gtatttttct
      961 ctcgtccatc catgaccata ttagcacgtg tatactcttt gtcagtatgc tcaacgacca
     1021 tatcaggata taagtaatac tctaagtaat tattcgggat attacttgga aaatttttcg
     1081 tgatgactga catcatttga aaggtttttt gccaagtttt atctcctata ttaaaatcgg
     1141 cgacttgcat ttcttttgtt attagttttt caataatctc tggcataatt tctcgttgca
     1201 attctttatc ataaatcgat gtataccagc caaagtggtt taaaccataa tacgtaacga
     1261 tccaattctt acgatcataa ccgtaattca ctgcaatcgt ttcttcaatt gaaatagtca
     1321 tatcacaggc attaatcatt tttacattcg gatatttacg acgaacagct tcagaaacga
     1381 tcgactctgg atttgtataa ttcaaaatcc atgctttggg ggcatagtct tgtatatgat
     1441 cgacaatttc caataaacct ttgattgagc gcatcccata agcaaatcct ccgagtccac
     1501 acgtttcttg tcctactaat ccatgtttaa gtggaatctt ttcgtccttt tctcgcatct
     1561 ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca tcctgtaaaa gcaagctttg
     1621 gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt tcttttcagc ataaatttaa
     1681 caatcaagta catatccata tttctttctt catcaatatc atataaacgt atttcagata
     1741 atggcaaccg tttttcattg ttcaaaatgg cttgaacgat tcctggtgta tacgtactcc
     1801 cgcctccagc tatagtgata atctgttttt tcatcatcat cttttccttt ctattcttct
     1861 aataatttat tgaattctgc cataacataa gtcacttcta agccgatgat aatttgataa
     1921 tttttttcag ttggtttaat gatcccgctt atccctaatt gcttgattcg ctgttcctcg
     1981 accatggtgg gatccactac ctctacccgt aggcgtgtat tacaactata gacatccagg
     2041 atattggctt ttcctccaaa cgcttgtagc atttttgttg ccatatattc ataattttta
     2101 cttgctaacc ctaactctgt ttcttctaag gttacttcat tgccaaactc tgccttagct
     2161 tcacgtccta atgtctgaat gtttctctta ttgatcaaat aataaaaagt aacgtaatac
     2221 aaagcaaaaa agaaaattcc gattggaaga atcaaaatag cattggtagc gattcgaaaa
     2281 ttgatgagat aatcaacaat acatgctccg aatgtaaaac caattcgtat attgaataag
     2341 taacagacaa tccctgctaa accggcaaaa agtgcatgga tcacgtacaa cataggagaa
     2401 agaaacatga aactaaattc aacaggctca gtgatatttg caataaatga agttcctgca
     2461 ccgcttccca tgattccttt gaccatctct ttattttctt tataagctgc tttatagata
     2521 gctagacata tagctggtat accaaacatc attactacaa aaaaaactga taaaaacagt
     2581 ccagcctgag gatctccgtt gataaagcgt gtcatctctc ctctgaaaac ttccttgcta
     2641 ggagatgtat agcttcctaa atcgtaataa atatacgtat tcaacacatg gtgcagtcca
     2701 aaggggatca gcaaacgatt caaaaacata aatatcccca cacctaatgc tcccatatca
     2761 acgagcagtt ttgcaaaatt atcaatccca atttggatga gcggccagat aaagcccaaa
     2821 aaaacagctg cgattgtttg tagaatcatc accatagtca atgggaattt ttcgcccgca
     2881 aaataagaaa aaactactgg taatttttgt tctttaaagc gattatacgt ccatgccgct
     2941 actaaaccgg aaataattcc accaaaaatc cccatattca cttctgggtc cataatttct
     3001 aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa gtgcaggtat tcctttgtcc
     3061 ttatttttga caaaaccgat tgcaactcct atagcaaata agatatccaa gtttccaaaa
     3121 acggcatttc cagctgcacc aattactggg atgttcagca tatcgtcagc tgataaacgc
     3181 atgatcaatc ccgctaatgg catcgctgca actggaagta gcatcgctct tccaagcttc
     3241 tgcacatttg atttaacatt catttttatt cctcctctat taaatatcct catatgttac
     3301 taactcaatc aaaggatcat ttttcacttt ttgagcaaat gctgaatgaa ttaaaaattc
     3361 gacttggtgg gtacgaggat acacaaaact tgaaccattt aacaattcct gatccaaata
     3421 cgctgcgtga cacatgactt cgatcgtttt atcttctctc aatatcatga gtaaacttgc
     3481 caaatattct tccacactcg tattgatttg tcgttttttt tcatcagttt ctcctacgac
     3541 atcgaaacaa ggctcaaaat aatcgacatg acgaacttca tcttttcgtt caaagttgct
     3601 ccgaaccggc aaatcatatt tttttgctaa tgtaacaact acctcctgat tttcatccaa
     3661 agtatgagta tgatgatgac tgtctaaatg agttggtatg atccccgcat gataaatttt
     3721 ttgaatctga gcattccatt cctgatacag ttgatctttg tcaatgttaa atggatgctg
     3781 ataataagat aaggatctga actctccgct ttcgataaga gtatcgacag ttttgaggag
     3841 tggtttgcca cacgtcaatg ttaaatggac tcctacacct agcctaggca tttcctttct
     3901 tagtttcaca gcatgctcga aacccggcat atttgccatc aatgtagttg atgtcaaaat
     3961 tcctcgctga taagcatcca taatcccata attcacacca tggctatagc caaaatcatc
     4021 cgaattaaaa atcacttttc ccatttcatt ctccttcttt tttctactaa aataggcgca
     4081 accaaataat catagtccat ttactatgtg tcatttggtt gcgcctatag atagttacgc
     4141 ccttatttta atagattctt gctagatgaa ttgccaaata gatgatttca ttttcttgaa
     4201 tggcaagttg aaattgctgt tcgatgatat ctttaatctt tagtgctgta caatacgctt
     4261 tgctatgttt aaatgtttca gtaaaaaaac caatattttc tactggcgtt tcttcctgat
     4321 aaatcaagcg tttaaccagg aatttgatat gaaccattaa gcgatgataa gtcagtgatt
     4381 ctttatcata aacgtttgaa taggattttt ctagatggga caaaatcgtg gtcgtcattt
     4441 ctactatttt taagctcttt aaactattag gctctcctag ctcagcatta atgatatgca
     4501 tagtgatgaa tcctacttca tttgacccta actcggtttg atatcgatga ttgattcgct
     4561 cgaccacttc tactgctata tcatattcat ttggataaag gaatctcatt tctggcaaca
     4621 taattgctgt gacatctcta tcttctcttt ttcgttgaat agcaaaataa atatgatctg
     4681 tcaatgtaat atagatattt gaatctaatg gagaggagat cctagctgtt atcagttcaa
     4741 tcacttcttc cgcaaaatcc ataaattcga ttggaatctg cgagactatc tcactgaatc
     4801 gtttgatctt ttctttagaa tctaatacaa ataatttgtc agcatcctca acatcaaatg
     4861 gcttactcac tttagcagaa aagccaatcc ctttccccat cgcaattttt tctattccat
     4921 cgtcatcgat caatacagca ttatgattca gctttttcat cacatacacc gttaattcac
     4981 ctcctctata tgataaaaca aaacaacttg cagactcctt catacatatt tttgtctcca
     5041 aacaattttt aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt cgtgcctaat
     5101 tgaatagtag caccctacat aatttacaaa aatagtttaa cacataataa aagcgttatc
     5161 aatatctttt ttactcgttt tatgataata cttgtatcga attgatttgg aggtaattac
     5221 ataaaaacaa aaaaaatata gcaactctct ataagaaagt tgctatattg ataattatac
     5281 cggcggccgg ggtcgaaccg gcacgtcctc aacggacact ggattttgag tccagcgcgt
     5341 ctgccaattc cgccacgccg gcaaatttaa aaataataac tggggtagct ggattcgaac
     5401 caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc aagaataaaa
     5461 ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc gttaaccact
     5521 tcgccacatt cgccatcata aaagatgtta tttaatttct caataaaaaa ctaagatgaa
     5581 gacatcttag tcaataccgg cggccggggt cgaaccggca cgccctcaac gggcactgga
     5641 ttttgagtcc agcgcgtctg ccaattccgc cacgccggca agttgattta ttagtactaa
     5701 ggcggtaacc ggatttgaac cggtgatgaa ggttttgcag acctctgcct taccacttgg
     5761 ctataccgcc attatactat tatcaatcta taaaataaag aataaattat agaaactggg
     5821 gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac cgcttggcta
     5881 taccccaaga ataaaaaggg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa
     5941 tccggtgcgt taaccacttc gccacattcg ccatggcagg ggcagcagga attgaaccca
     6001 cactaacggt tttggagacc gctgttctac ctttaaacta tgcccctata aaatggagga
     6061 gagtggattc gaaccactga accctaagga acggatttac agtccgtcgc gtttagccac
     6121 ttcgctactc ctccatggtg gcgcgggaca gaatcgaact gccgacacac ggagcttcaa
     6181 tccgttgctc taccaactga gctaccgcgc catattttaa aattattaat tgaataacaa
     6241 gaacgacggt tccgacggga atcgaacccg cgatctcctg cgtgacaggc aggcatgtta
     6301 acccctacac cacggaacca gttcattatg gaggttaacg ggatcgaacc gctgaccctc
     6361 tgcttgtaag gcagatgctc tcccagctga gctaaacctc caacattcaa ttaaaatgac
     6421 ccgtacggga ttcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc
     6481 aacgggccag gtaaatttgc tacggagagt aagggattcg aacccttgag acagtttggc
     6541 accatctaca tgatttccaa tcatgctcct tcggcctctc ggacaactct ccagagtgaa
     6601 agaagctcaa tactctttct caaaatgag
//
LOCUS       DASNNU010000116         6527 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.117, whole genome shotgun sequence.
ACCESSION   DASNNU010000116 DASNNU010000000
VERSION     DASNNU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.117"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            107..793
                     /locus_tag="U8116_002476"
     CDS             107..793
                     /locus_tag="U8116_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEN8435410.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            795..1739
                     /locus_tag="U8116_002477"
     CDS             795..1739
                     /locus_tag="U8116_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEN8435411.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1938..2582
                     /gene="pcp"
                     /locus_tag="U8116_002478"
     CDS             1938..2582
                     /gene="pcp"
                     /locus_tag="U8116_002478"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEN8435412.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2661..3938
                     /locus_tag="U8116_002479"
     CDS             2661..3938
                     /locus_tag="U8116_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435413.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            4196..5413
                     /locus_tag="U8116_002480"
     CDS             4196..5413
                     /locus_tag="U8116_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN8435414.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(5734..6483)
                     /locus_tag="U8116_002481"
     CDS             complement(5734..6483)
                     /locus_tag="U8116_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEN8435415.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2105 a         1210 c         1361 g         1851 t
ORIGIN      
        1 cgaaaaatat ttaaaaatag tgtctatttc gtgagaaaac aactatattt tttatgtgtt
       61 ttgctttata attaaaaaaa tattgtcatt ttatggagga aaatatatgg aaaattatct
      121 tagcctttta ggcgtcttga ttgttattct cgggtttgca tttaaattag actcgatatt
      181 gatcgtaatg gttgctttag ttgttacagc tttgactggc ggtcttggca ttcaaggaat
      241 gttagaactt ttaggtacaa gttttgtaaa caatcgcggg atggcgattt ttatcatcat
      301 tatgttggca acaggaacgc ttgaaagaaa tggtctgaaa gaatcagcgg cttccttgat
      361 caagcgattc aagaaagtat ctgctggtat gattgtagat atttatggga tctttcgaat
      421 gatctttgcg gcttttaacg tgagttttgg tggagttgct ggatttgttc gtccaatcat
      481 tttaccgatg gcattgggga ccatcgaatc aaaagggtta gaagtccatc ctaagcatga
      541 agaagaacta aaaggtatgg cttcagctat ggaaaatgtc tgctggttct ttggccaagt
      601 actattcatc ggaggagcag gggcgttgct agtccaatca acactgaaag atctaggata
      661 tgacgtaaca ttgggacagc ttgctttagt ggaagttcca gtggctatca cggctttagt
      721 tgtggcaagt gtctacttct atcttaaaga aaagaaatta gcgaagaaat attatccagg
      781 aaaggggcaa taagatggac ttttttacaa atcctgatat tttattaggc gataaattac
      841 tggagatcct ttatatcgtg atgggactcg tattgattta cactggagta agaaatttaa
      901 cagataaaac caatcctcat cgttatgggt cagccttttt ctggacggtc ttaggggtag
      961 ttattgcagg aggaagatgg cttccggcaa tcgtgaacgg tggcttgatc tttgcgatga
     1021 ccataccagc aattgctaag aaagtcagca aaggagaaag ccggcttcca tcaaaagaat
     1081 atatggaaaa aatgtcagac aaactaggaa tgaaaatttt tattccagct ttgagtatcg
     1141 gtgtctttgc gatcttgttt gctctattta ccaatttagg tgctttagtc ggcgtgggcg
     1201 tcggtgtttt tgcagccatg ctaatcttga tgttcttttc tcgagataat cgaccaagta
     1261 catttttaga tgatgcagca gatatgctgg gcacagtagg gccgttaagc atgttgccaa
     1321 tgcttttagc ttctctaggt gcggtattta caagcgctgg agttgggaca gtgatttcca
     1381 atgcagtagg tacgatcgtt ccagaaggaa atgtgaacat cggcatcatc atttacgctc
     1441 ttggcatggt gatatttacg gtaatcatgg ggaatgcatt tgcggccatc accgtgatga
     1501 cggttgggat cggtgcacca ttcgtattta gtcatggagc gaatccagcg ttaataggaa
     1561 tggttgcatt gacttgcggt ttttgtggga ccctgctgac accgatggct gctaacttca
     1621 atatcgtgcc tgtagcgatg ctggagatga aagacaaata cggagtgatc aaaaaccagt
     1681 tgtttattgc gttatttatg ctagttttcc aaattgctta tatgattcta tttaaataat
     1741 agaagagagg ggttgtgaca gaataattgt cgcaacctct tctaacattg tagtggaaca
     1801 ctattttttt ataagaatat attaagtttg atctgttatg ttatttaaac tattgtttcc
     1861 tttatttacg ctgttcaagg aaaatggtat gataaaagat aaccatatgt taaatcaatc
     1921 ggacggagga acaacagatg aaagtactag taacaggttt tgatccattt ggtggggaca
     1981 aagtgaatcc agcctatgaa gcagtaaaaa aaatgccaga tgaaatcagc ggtgctgaaa
     2041 tcatcaaagt ggaagtaccg accgtattcg aaaaaagcag tcaagtagtc aaagaagcca
     2101 tccaacagca ccaaccagat gtcgtgatct gtgtcggaca agcaggtggg cggtcagcag
     2161 tctcgttcga acgtgtcgca atcaatctag cagaagcaag aatccctgat aatgaaggaa
     2221 atcagccatt cgatacggct ttagaagaaa acggtccagc cgcttacttt acctcattgc
     2281 caatcaaagc aatgacaaaa aatgtacaag atcatggtct accagcgtac atctcttata
     2341 cagcaggaac atttgtctgc aatgatatca tgtatcgttt gcttcatttg atcgaaacac
     2401 aattcccggc aattcgaggt gggttcatcc acgtgccatt ctcacccgat caagtgatcg
     2461 atcgtccagt gggtacgcct tctatgtctt tagaagacat tgcttcttct ttgacttatg
     2521 cagttgaagc agcagtagaa aataaagaag atatttccgg aaatgccgga acaactcact
     2581 aatttagcaa gaaacactgg ctggttgcta acgttatgtt agtagccagc tttttgtaat
     2641 tagaaaggaa agaaacagcc gtggaagaat ggcaatccgt ctttgaagaa tggtttccaa
     2701 aagaaatcag caaaagctat cccatcaaaa tttctaaaca atacacaagc agtcaacgtt
     2761 gggagatcta tgcaaaatta acgaaaaaac agcgggaact agtcgataag caccgccgat
     2821 atctaatcag ctctcgtttt atggaagaac attatttagc tgcaacagat tgggtgtttt
     2881 ctgacttcaa gatcaatccg ttttttcgaa caaaacgaag ccagcaaaaa ttgtattgtg
     2941 agtgtggtag agaattaaaa gtgcaataca tcgttaaatc gccaaaaacc ggaaagatcc
     3001 taaaactagg catcaaccac tttgccgatc acctgcatgt ctctccgaca gtggcagctt
     3061 cgatccatca gggaatgaca aaagttgatc tggccttaga cgaattatta tggcttaagc
     3121 agaagaatat cgattttcct gaaggactat ggcaaaaata ctgttttgtc ttgtaccaaa
     3181 atcgtcggat gaaacagcca tatttaccgg atataaaatt agctcagcgg ctcgcagaat
     3241 ttcgccaagt agaaatgccg atttacatag cggattatca agcattggaa aatgaaatca
     3301 agaaaataag tgaacacatc aatgggcagt caaaaaagcg acagataaaa aaagaactat
     3361 ttgacgactt tgcagaagaa ttagtaaagg atgtagaaga attcttgatc aactatcgcg
     3421 catttttacg taaagattgg caatccatcg tatatgaaga agtgccagta catcctaatg
     3481 cttattttga aacatttatt tccgtgttga gaaagacaaa acgtcaacga acaccagaag
     3541 tcactgctca gatggaatat tttgcgaaaa accaacgatt catccaacca aaaatctatc
     3601 tatttatttg gaagcaatat tgtcgctatg gttttacaga agggttcttc gacagcattc
     3661 ctcggatagt cagaaatggt tttttaaaag tattgcgtaa agaacgagaa gctatacaat
     3721 ctgctgataa gaaagaccgt acagtctcaa aagaaaaatg gcaactagta gtgaaagata
     3781 tccaatctgg aaatgtgcaa gagacaatcg acaaatggaa aggaaaacat tatcgtttta
     3841 ctgaagctca gaaacaagcg ttggaatatt atcagaagtt agaagaatca ctgcgtttca
     3901 atgatgaggc aagaaagtat ctgaaggaat tactataaaa aacatactaa gattagaggg
     3961 gaacatcatt gacgaatgat gttctcctct attttctatt tttagattat agtgaagagt
     4021 gaaagaaagt tcgaacaaag ccttgaggac tacgagtccc tatcaaaaac cgaacttctt
     4081 tactgtcatt ttagaatcag gtttacgtat gttggggcca tgagcccgtg tataggaaat
     4141 tggaatcgaa atgaagcagt aaagttcttt caaaacttaa aaaggaggaa aacaaatgcc
     4201 ttacattctt gcattagatg tttcaatggg acacagctac agtgtcctat acaaagacga
     4261 cacctgctta tttgaagacg agattgaaca cactcaaaga ggatttcatg cattactcga
     4321 agagatccag cacttgcctg agcgtccatt gattgttttt gaagcaacgg ggatttattc
     4381 cagaccagtc gaaaagttct ttcaagatca tcacttttct tactgtttgt taaacccgtt
     4441 agaggcgaaa aaacagatgg aagaaagcac cttacgcagt tggaaaacag ataaatcgga
     4501 tgcccaccgg ctcgcacaga ctcatcttaa aaatcaacgt cagccaaaag aagtacaaag
     4561 caatttttac ttagagatgc gcgatttagc ccgtttttat caagaaattg agaagaaaat
     4621 cactcgtctg cgcatggatt tacacaactg ccttcaattg acttttccag agcttgaaca
     4681 gttcttttca aacaggctga ccccttacgc attgacattg atccgtcttt ttccccatcc
     4741 agattttgta ttggcatcta ctcggacaaa aataaaaaat aaactgatca acgaaacacg
     4801 gaaaaaaatc tccgcgaatc gtgccgagca aaaggcagat caaatcatcc actatgcaca
     4861 gtgtgcctat ccagctgttg aaaaagacag tatccactgt caaaaaacga tctattacgc
     4921 cgaacttctc caagatttac tcgaacaaaa aaaagcactg gcgacacaaa tgatcaaaaa
     4981 agccgaaggc tctccttgct ttttgttgta ccaaacgttc cctgggattg gcgcactcac
     5041 agccgcttta ttgctcggtg aattaggaga tatcacacga tttaaaaccc ataaacaatt
     5101 gaatgccttt gtcggaatcg acatccgacg gtatcattca ggtaagtata ctggccaaga
     5161 ccgcatcaat aagcgtggta atcccaaagc acggaaaatt attttcttca ccattcgaaa
     5221 tatgattcgc cagcaacgcg cagctcctaa tcatattgtc gattattact acaaattaaa
     5281 aaagcaacct atccctaaga aagataaggt tgccacagtt gcttgtatga acaagcttct
     5341 gaaatgtatg cacgctatgg ttagggcaca tacagagtac gactatgcgt acgcggtctc
     5401 agtggaccat taactaaacc acttacagta taccacttct ttgcccagat cgaaaatttt
     5461 ttacgaacgg ggctttattt ggtgtgcttt gattttgtcg ctttcgttta cgagctattt
     5521 ttcttgcacc ttttacttga ctaatcgtag gaaatagagg ctgggacaga agcgtttaac
     5581 tccaagaaat aagaagaaat tcacgaaaat tgcttttcaa atttttgtga atttcagctt
     5641 atttccgaag gagttgcttc tgattccgcc gtttatacgt gtttagagcg tgagacaaaa
     5701 gtgttcttta cttttgtccc acgctcagtt tttttatgcg tgttgttgtg cgttgtagta
     5761 ttcttcttcc attttttgca tacgttcacg ttctttttgg ttataccaag gtgaaacggt
     5821 aaatgcaagg acatcattca gcaagtaaac agagctgttc acgaacatcg ccaaacttgc
     5881 agatccatgc gtaaatgaaa tataccaaag gaccatttga gcaagccctg aagctaacca
     5941 ccagtaatat tggttgttgt aacgcaagaa actgatgata ccagcagtca agctaataga
     6001 aaaagcaatg gcatctaccc acggccgagg atcatctgta taacgaccaa tcaggaatcc
     6061 tgaaacaaga tagacaagga aagtactgat gattgcgata aaccagcttt ttccagtgaa
     6121 ttttcggatt tttgaagcca tattatgatt ccattcttta cttaacaata cggggatatc
     6181 caatgtgatc atataagcaa tctgttcacc gatacttaaa tagttctttg ctgaaaaccc
     6241 aacgataata aaacaaatcg cagacaaaat acctaaaata ccgttgactg attttgccgc
     6301 attgatggat aaaatacaca atacaccaag cgtggtaccg ataaatgtaa taacggacag
     6361 ccaagtgatg ggactattga caagagtcat gacctggcag ccaagactga aaaagaaaag
     6421 ataataattt tgttgcggcc agccttttag ctgatgagct aaaaactgaa aatatgactt
     6481 catgtatgag gactccttaa gacactaaat gtagtgtgtt ctgtatt
//
LOCUS       DASNNU010000117         6331 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.118, whole genome shotgun sequence.
ACCESSION   DASNNU010000117 DASNNU010000000
VERSION     DASNNU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.118"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(247..792)
                     /locus_tag="U8116_002482"
     CDS             complement(247..792)
                     /locus_tag="U8116_002482"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN8435416.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(796..924)
                     /locus_tag="U8116_002483"
     CDS             complement(796..924)
                     /locus_tag="U8116_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN8435417.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            complement(917..2008)
                     /gene="phnW"
                     /locus_tag="U8116_002484"
     CDS             complement(917..2008)
                     /gene="phnW"
                     /locus_tag="U8116_002484"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEN8435418.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            complement(2225..2704)
                     /locus_tag="U8116_002485"
     CDS             complement(2225..2704)
                     /locus_tag="U8116_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEN8435419.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            3063..3845
                     /gene="rpsB"
                     /locus_tag="U8116_002486"
     CDS             3063..3845
                     /gene="rpsB"
                     /locus_tag="U8116_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEN8435420.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3944..4825
                     /locus_tag="U8116_002487"
     CDS             3944..4825
                     /locus_tag="U8116_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEN8435421.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            4961..5683
                     /locus_tag="U8116_002488"
     CDS             4961..5683
                     /locus_tag="U8116_002488"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEN8435422.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            5686..6243
                     /gene="frr"
                     /locus_tag="U8116_002489"
     CDS             5686..6243
                     /gene="frr"
                     /locus_tag="U8116_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEN8435423.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         2113 a         1177 c         1277 g         1764 t
ORIGIN      
        1 cgaataaacg gtgttcaaaa gcttgcatct gcggtaataa gcctaaccaa acgaaaaata
       61 tgaggagcta tttttgcttg gctgttttta ttacttgata caggacagct ttttcacaac
      121 ctcttatttt gtataaaagt cattctggat tcttgtcagc gtacaaaatg tattctgtta
      181 actcttttaa attgtttaca caaatatcag cgccggcttg ttggaatcta agagcaacag
      241 actgttttaa acgatctttt tccccatctc taatttttgg tactcatcat aagaaatccc
      301 cattagcgaa ctaccttcaa ttacacctac tgtcaacact ccggcatttt tcccttcctg
      361 gatatctgag accgtatctc cgactttgat tacttcatca accgacttgt ttttgaagta
      421 ctgcatgttt ttatggatca tataaggaaa cggtcgacca tacccttcga cgtcttcaaa
      481 agttgccatg aagtctggtg aatacctttc tttttttgcg gtttctgcta cgattgtcat
      541 cataggagca ttaaatccat tcgtggaacc aatattgatc ccagcgtttc tcaaccgttc
      601 gatcgtttcc aatgtgtcgg gtttcaaggt agaatgcttg accaaatctt taaacagata
      661 atgttcaaat tgttgataca attcctccac ctctttttcc tgaggtagtt gtccataaat
      721 agctatccat tgttttttga cagaagggag ggaaagaagc tgttggatat gatcccattt
      781 taattttccc atcggttagc ggatttcttt gtcagtcagt tgggttcctt tttctagaaa
      841 agcatttcta aaagcgtgga caggagccat acagccaaag tccacagttg ttcctgccca
      901 atcaaaaatt acagttttaa tcattaacgt tctccttata gtctttgata attgctgtca
      961 gttccaaaat atcttccgga tagatctcgc caatatttcc gatacgaaaa cattcatgat
     1021 cgctgatttt cccgggatag atcacaaatc cattttgctt taaatactga taaaaatgat
     1081 taaaatcaaa tcgccagtcc ttagggtaaa gaaaagaggt gatgaatggc ccctgatatt
     1141 cttgaatgaa aggctggaac cccatctctg tcattttttg tcgtagcaca tgattattgg
     1201 cgagataacg tgaatatcga gtttcgattc ctccttctgc ctctagttcc ttcagcgctt
     1261 gttgaaaagc caaaaccgta tgggtagggg aagtgaaacg ccatttgcca ttctcgtttt
     1321 ccatcacgcg gtactggtca tatagatcaa gtgacaaact tcggctaaca ttttttcgtt
     1381 tttccagttc cgctttatta gcaagtacaa aagaaaagcc tggaacacct tgaatacatt
     1441 tgtttgagct ggaaatcaaa aaatcaatcc ctagttcggc tacacaaatc ggtatcccac
     1501 caaagcttga catcgcgtcc actataaaca aaagatctct ttcttgtgtg acagcagcaa
     1561 tctcttccaa attattcaaa ataccggaag tagtctcgct atggaccatt gctagtgcgg
     1621 agatagacgg attctcttca atcagctgtg taatcacatg agcttggagg atttgatgtt
     1681 ccgatacctc ataaacggta tagttcagtt tcagacattc gcacatgttg atcattcgtt
     1741 gtctatatgc gccgtttgaa caaatcagta cagtatcttc tggcctgatc acagaactta
     1801 ataccgattc taccccgaag gagctgctac cttgcattaa gaccgttgtg tatattcctt
     1861 ccgatacttg agcaagatcc aataattttt ttcttatcca ttgtgtaatt tctttgtatt
     1921 cttgatccca agtacaatga tcgatcagca tagcattttt cactgtttca gaagtcgtca
     1981 atggtccaga agttaataat ttatacatat gatttccttt catttctttt ttgaaataca
     2041 caaagagcaa gaagctgctc ttgttaacat ccaatataaa tgatgattat tgtgattcaa
     2101 tcaaggtgtt gaaaaatttg aataaaaaat gatgtatttt tgtaaagagt gtaaaggttt
     2161 attcaataag gaaaatattg aaaataaaaa aacacgcatt ccatattagg aatacgtgct
     2221 ctgattaaaa aagtgatact tcagttggat ggtgaagaaa ttcttctact cgttttgcgt
     2281 cctcctcatt ttccgagatg accatgatcg tatcgaatcc agcaattgta ctgacaatag
     2341 agggaggttt gatctcatct aaaacagatg caaataaatg agcattgtct ggcaaggtat
     2401 tgacgatcgt caaaaagtgg actcgttcga ctttcaatac aatgtcttca atcatttgaa
     2461 ccagccgatc ctcttcctca gtcctagtct caggagttcc tttttcgcga aacaatacga
     2521 agcgggaaac gccattttca tcaggtgcct tcacgatttt tagatcacgg atatctcgag
     2581 aaatcgttgc ctgcgtggcg gtaacgccat aatgcccaag tttgtttaat agttcttctt
     2641 gcgtacggat cgggaattct tcaatgactt gtttaatcag caaatgtctt tgagctttgc
     2701 gcattttctt tctcctttac gaaagatttc acatgtatca taacaaaaaa aagaaggatt
     2761 gcccaataat attcgtcttt tatacctaaa attattttat ggaaaaagga taataaagaa
     2821 acaaagagaa atctactgtt tttcttgaat tctttttatc tttcaggtaa actattgaat
     2881 gttgagcaga aaagtatatt tgctcttgcg atacggatct tttgtctgta taatagctga
     2941 gggtgcttta tgcatcagca aatccacatt cttattggat tgtcggtagg tgcttgaaaa
     3001 agtttgccgg caaggtagaa gataagaaga ggaaaaataa aaccaaatcg gaggaaacaa
     3061 acatggcagt aatttctatg aaacaattac tagaagccgg cgtacacttt ggtcaccaaa
     3121 ctcgtcgctg gaatccaaaa atgaagaaat atatcttcac agaaagaaac ggaatctaca
     3181 tcattgactt gcaaaaaaca gttaaattag tagatgcagc ttatgattac atgaagagcg
     3241 ttgctgaaga aggcggcgta gcattattcg taggtacaaa aaaacaagca caagaagcaa
     3301 tcaaagaaga agcaactcgt gcaggacaat acttcgttaa ccatcgttgg ttaggcggaa
     3361 cattgacaaa ctgggatact atccaaaaac gtatcagccg tttgaaacaa atcaatgcaa
     3421 tggaagaaga tggaactttt gaagttctac ctaaaaaaga agttgctggt ttgaacaaag
     3481 aacgtgaacg tcttgaaaaa ttcttaggcg gtatcgctga tatgccaaga atcccagatg
     3541 taatgtacat cgttgaccca cgtaaagaac gtattgctgt tcaagaagca cacaaattaa
     3601 atatcccaat cgtagctatg gttgatacaa actgcgatcc agatgaaatc gacgtagtaa
     3661 tcccttcaaa cgacgatgca attcgtgccg ttaaattgat tacttcaaaa atggctgatg
     3721 ctttcatcga aggaaaccaa ggtgaagatc aagtagttga agaagatttc acagaagaaa
     3781 acacagcaac atcaatcgaa gaaatcgtag atgttgttga aggcgacaat tcttcagcag
     3841 aataattttg aatgatggag ctgtttcaaa ggctgggcga caagcctcac tctcctttga
     3901 agcagctttt tttaaaaaga aaaacacaca ggaggagtta aacatggcag acgttacagc
     3961 taaaatggta aaagaactac gcgaaatgac tggcgtagga atgatggatg cgaaaaaagc
     4021 attagttgaa gtagaaggcg acatggaaaa agcagttgat cttttacgtg aaaaaggaat
     4081 ggcaaaggct gcgaagaaaa atgaccgcat tgcagctgaa ggattggctt cagttgctgt
     4141 gaaaggtaac actgcagcaa tcgttgaagt aaactcagaa acagactttg tttctaaaaa
     4201 cgaaatgttc caagatttag taaaagaaat tgcagaactt gttgctgaaa acaaaccagc
     4261 agacatggaa gctgcaatga aaatcaaaac agataaaggt acaattgaat ctgacttgat
     4321 cgaagcaact caagttatcg gagaaaaaat cagcttccgt cgctttgaag ttgtagaaaa
     4381 agacgacaat gctgcatttg gtggatactt gcatatgggc ggacgtattg ccgtattgac
     4441 tgttctagat ggaacaactg acgaaacagt tgcaagagac gtagctatgc acgtagcagc
     4501 tatcaatcct cgttatgtaa acgaatcaca aattccagaa gctgaattag aacatgaaaa
     4561 aactgttctt acagaacaag cattgaacga aggcaaacca gcgaatatcg ttgaaaaaat
     4621 ggtagaagga cgcttgaaga aattcaaagc agaaatcgct ttggtggacc aaccattcgt
     4681 taaagatcct gatatgactg ttgaaaaata cgttgcatca aaaggcgcaa ctgttaaaac
     4741 attcgtacgt ttcgaagttg gcgaaggtat cgaaaaacgc gaagataact ttgtagaaga
     4801 agttatgagc caagtgaaaa aataattctg ttgaataaca gacgggaacg cacggtttgt
     4861 gcgctccctt tttttaagca aaacaagaaa aaccagtctt agagtgtcga aatcatctaa
     4921 gtatggtaaa atggcaatag aaaatattgg aggaatgaac atggtcacac cgaaatatca
     4981 gcgtgtagtc ttaaaattga gtggagaagc tttagctggg gacgcaggat ttggaatcaa
     5041 accaccagta atcaaagaaa tcatccaaga aatcaaagaa gttcacgagc tgggcgttga
     5101 aatggcaatc gttgtgggcg gcggaaatat ctggcgcgga caaatcggag cacaaatggg
     5161 catggaacgt gctcaagctg attacatggg tatgttagct acagttatga atgccttagc
     5221 tttacaggat acgttggaaa acgtgggtgt accgacacgc gtgcaaactt cgattgaaat
     5281 gcgtcaaatc gcagagccat atattcgccg taaagcagaa cgtcatttag aaaaaggacg
     5341 gatcgttatc tttgcaggcg gtaccggcaa cccgtacttc tcgacagaca caacagcagc
     5401 attacgcgcg gcagaaatcg gcgcagatgt tatcctgatg gctaaaaaca atgtagacgg
     5461 tgtttattct gctgatccga aagtagatgc aaatgcagta aaatttgaag aactgactca
     5521 tctggaagtt attgccaaag gattacaagt catggattca actgcaagtt cactaagcat
     5581 ggataatgat attccactgc ttgtcttcaa tttaaatgaa cacggaaata ttcgccgtgc
     5641 aatcttaggc gaaaatatcg gaacaactgt aagggggaaa taaaaatggc agatgcaatt
     5701 atgacagaag caaaagaaaa aatgaaaaaa gcagcacaaa atcttcaacg tgaacttggg
     5761 caaatccgcg caggccgtgc gaacgctagc cttttggacc gtattactgt gaactactac
     5821 ggtgcgccaa caccattgaa ccaaatggca tcaatccaaa ttccagaagc acgtgtttta
     5881 atgatcacac cttttgacaa gagcattctt caagatgttg aaaaagctat tatggcaagt
     5941 gatatcggaa tcagtccaac caatgatgga aatgtcattc gcttagttat cccgcaactg
     6001 acagaagaac gcagaaaaga attagctaaa gatgtgaaaa aagaagcaga aaatgcaaaa
     6061 atcgcgattc gtaacatccg tcgtgatgcg atcgatgaat ataaaaaaca acaaaaaaat
     6121 ggtgatatta ccgaagatga tttgcgcgga ttagaaaaag acgtacaaac attgacagat
     6181 aacagcatca aagaagtaga taagatcgct gctgataaag aaaaagaatt attggaagtt
     6241 taataaaatc ataaaaacag aggggcggat cattccgctt cctctgtttt tatttatatt
     6301 tcttttattt aaaaattaaa aaaagaaaaa a
//
LOCUS       DASNNU010000118         6173 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.119, whole genome shotgun sequence.
ACCESSION   DASNNU010000118 DASNNU010000000
VERSION     DASNNU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.119"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            725..811
                     /locus_tag="U8116_002490"
     CDS             725..811
                     /locus_tag="U8116_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN8435424.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="U8116_002491"
     CDS             complement(932..1621)
                     /locus_tag="U8116_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435425.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="U8116_002492"
     CDS             complement(1628..1906)
                     /locus_tag="U8116_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435426.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="U8116_002493"
     CDS             complement(1866..2780)
                     /locus_tag="U8116_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN8435427.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="U8116_002494"
     CDS             complement(2762..3142)
                     /locus_tag="U8116_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEN8435428.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="U8116_002495"
     CDS             complement(3592..3879)
                     /locus_tag="U8116_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN8435429.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="U8116_002496"
     CDS             complement(3899..4123)
                     /locus_tag="U8116_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEN8435430.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="U8116_002497"
     CDS             complement(4578..4748)
                     /locus_tag="U8116_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435431.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="U8116_002498"
     CDS             complement(4763..5296)
                     /locus_tag="U8116_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN8435432.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="U8116_002499"
     CDS             complement(5317..6159)
                     /locus_tag="U8116_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN8435433.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DASNNU010000119         6133 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.120, whole genome shotgun sequence.
ACCESSION   DASNNU010000119 DASNNU010000000
VERSION     DASNNU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.120"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(31..1203)
                     /locus_tag="U8116_002500"
     CDS             complement(31..1203)
                     /locus_tag="U8116_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEN8435434.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            1593..2408
                     /locus_tag="U8116_002501"
     CDS             1593..2408
                     /locus_tag="U8116_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435435.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            2534..2791
                     /locus_tag="U8116_002502"
     CDS             2534..2791
                     /locus_tag="U8116_002502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435436.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(3043..3615)
                     /locus_tag="U8116_002503"
     CDS             complement(3043..3615)
                     /locus_tag="U8116_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN8435437.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(3631..4236)
                     /locus_tag="U8116_002504"
     CDS             complement(3631..4236)
                     /locus_tag="U8116_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEN8435438.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(4357..4614)
                     /locus_tag="U8116_002505"
     CDS             complement(4357..4614)
                     /locus_tag="U8116_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN8435439.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4607..4876)
                     /locus_tag="U8116_002506"
     CDS             complement(4607..4876)
                     /locus_tag="U8116_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN8435440.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(5007..5186)
                     /locus_tag="U8116_002507"
     CDS             complement(5007..5186)
                     /locus_tag="U8116_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435441.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(5567..>6133)
                     /locus_tag="U8116_002508"
     CDS             complement(5567..>6133)
                     /locus_tag="U8116_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435442.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         1860 a         1172 c          983 g         2118 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc ttttatcttt tacacaatta tatggacttt atcaaaaaat aagaaattca
     1321 aaaaagaaaa aactttcctt aaaaacaaaa aataggagta ttcagtttct taatagtttt
     1381 gtaatagttg tatgtagaag ttttcttaat agtttttgta ataatttatt aatcgtagtt
     1441 tatataactt atttgtaagt gactagagaa ttaaataaaa aataattact atctagtacg
     1501 aaattaatta cataggataa tatattaagg tgctggacta caagtaaaaa atactgtatg
     1561 atcatgacag attcaagaaa ggagcaataa gattggttaa gatagcagga ctttctaaag
     1621 gtggaaaaac cgtttcacaa ctttataaac aaaactacag agactggaga cagttcagaa
     1681 aaaacattaa ggattcttac tttattctac caacagaact tgaaaaatat ttaccgaata
     1741 acaaagctat taatttatat ctgtattact gttttaaggc aaagaatgaa ggcggggatt
     1801 cctggcattc ggtcaatacc atagcagagt ctctaggagt gacaactaaa actataaaca
     1861 cctggaataa aaccttagtt gatttaggta tgattgcacg tatagacgat aaccatctca
     1921 gtaaatcgac aattttactt cctctctcta gttactataa attagccctt gatagttctt
     1981 taaaggatgt taccgagagc actgtccatg aaattgatgg caacttggtt gcagtttatc
     2041 atttgtttga atggagaaaa aatgaggctg atgaaaatta taacgttcct tataacacac
     2101 tctgttgtgt ttttaaaaga aaaacacaaa aagatactat ctataaattt atcttggtcc
     2161 aagatgagaa aataagtaat ttcgaattaa gcactccttc tagtaagatt tatgacgatg
     2221 catatagatt tgaatgtcaa gatctagaag ccctcctttc cgcaaataat ctaaacaata
     2281 tagaacatga aaactttgtt gtaaacacta gatttaattt agtttctgaa aaggactcag
     2341 atttgttaga tttgctgata gagcttacta aaaatctaga taagaaagaa aacattactg
     2401 tactataact agcatcggtc catcattaag tttggactat tagaacactt gctattacag
     2461 aaaaaggtat gctagccacg cttactattt attggtatca aacaagtatc ttaatttgaa
     2521 aggaagatga tttatggcta atttatatat agagaacaaa ggagacttga atgccattat
     2581 tactttaatc gtagttatgg taaaatcaat aggtaatatc acaataggaa tgctgtttat
     2641 tatattagca gcagtgctga tttttatgat agttatttgg ttcttaaatg aaagatctaa
     2701 agacaatcaa caccgtaggg atatggagat aaaatcaata gagtcaatag attatttatc
     2761 gataataaca ctaaaaaagc cacgttccta aacgtgactt acgctgaacg atatgtgttg
     2821 tttggtagac gaacaaatcg ttcattataa atatatgatg gtatcctaat atagtaagcg
     2881 tggctagcat acctttaatt actttacctt ttacagttac catacacatt ttttgagcag
     2941 aaatcaagta aataaaaaaa tattatattt cacaacatgc taatagactc atatctagaa
     3001 cgcattaagt atttacctgt ctggttcgct gagacttctt ttttaacttt cttcaatttt
     3061 cttctttatt ctaaaaagcg tactgcgact gaaaccggtt atagcttcaa cttccttata
     3121 cgatttacct gaagtaagca gttcatatgc gtgcctcttc ttcggtgtga ttgttgcttt
     3181 cggacgtcct tctctgaaat taggattatt ctttcttgca aacatttttc cttcttgggt
     3241 acgttccaca atcatatcac gttccatttc tgctacactc aacaacgtcc gtgtcaccat
     3301 tcgccccatg gacgtatttt caatcgttcc tagattcaaa accttgatcg ttacgttatc
     3361 atcgagtaaa ggttcaatga tctctaatgc ttctctcgtg ttacgagcaa atcgatcaag
     3421 cttcgtaaca actatttcat ctccaggctt cagaccagcc aacagctgat tgaattttgg
     3481 ccgatcccgt tttgtacccg taaatttttc tgaaaaaata ttcttcttct ctacgccgtt
     3541 ctcaagcaac tgttgaattt gttcttttgt actttgagaa gcggttgaga cacgtgcata
     3601 tccatatttc gtcatttggt ccacctactt ttacaaatca tctatcgtat actcagaata
     3661 cccttcatag aaatagctga catcgattcc tttcatatat aactcgcgat cgtcaatttt
     3721 atcagtcaga gcattggaaa ttagatagcg tatttccaaa tcgtttatgg gacttcgctc
     3781 cattgcagaa agatagtctg ctttatcgat taagttccaa tctacaactt tttgcaattc
     3841 ttcctttaaa atgagatcca gccaaattct agtacttcgg ccatttcctt ctcgaaatgg
     3901 gtgagcgata ttcatctcca catatttctt gataatctct tcaaaagaag tttgtggcat
     3961 ctgatcaata tgctgtaacg aatgttctaa atacataact ggtgcaaatc gaaaatttcc
     4021 ttttgcaata ttgaccgatc ggatttttcc ggcaaaatcg taaatatccg aaaatagaaa
     4081 ttcatgaatt tcagataaac ctttaaatgt accaacttcg atttcattta tttttccaca
     4141 atcgtacaat tctttcgctt tttgtttact gagtttttct tcaaccttag ccaactcaac
     4201 ttgattggtt aatcccaatt tattctctaa aatcatgcga aatctcctta actttatgac
     4261 ataagtttat gaaacactct caaccccatt atatcgcgat tttgtttatg tgtcaaaaac
     4321 tattaagttt ctgacccttt ccttacttcc gattggttaa tagtgatctt ttgcagaata
     4381 aataaatatc gtttcatttt caactctata tatcagtcta tgttcatctg taattcttct
     4441 ggaccatttt ccagataaat catgctttaa tggctcaggt tttcctaatc cagcaaaggg
     4501 ggaacgatcg atatctttta ttaacttgtt aatctttttt atattgcttt tgtttccttg
     4561 ctcatgccaa taaagataat catcccaagc atcatcagac caagccttaa tcatcagatt
     4621 caacctcgat taagtcatgt gttttaaatg cacctttgga gaattgttca tctcctcgac
     4681 gaattttttc catgacgtaa ttattagaaa gtgttctcaa cgtttcttgc atagaatcat
     4741 aatctctttt tgataataca acaactgtat cttctacatc tttacttgtt acaataagtg
     4801 tttcagcatc ctcattaact tgtttcatat aactacgtaa attttggcgg aaatttgaat
     4861 aagctactgc ttccattcct ttcacccctt atttatttct ataaaacaat tgtacattat
     4921 attgtacaat taagcaaatg attaatattc taacttctta taattaaaag tgctatcact
     4981 tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc aaatcctgct
     5041 ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc acttttgccg
     5101 tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt aacgggtaac
     5161 cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa taaccctatg
     5221 aaaaatatgg ctgcaatcat cttgaatagt agactcacaa gggctaatac aaagcgccaa
     5281 aagatcaaac aaaataccag tattgccaaa aatactacga agtttaaatg cacagctgca
     5341 gcatggcgca taagtatttc gccaacaaat aggactaaca atagaggaat gattttattc
     5401 atatggatct ccttctttct cttaggttat cgggtgttgc ccgcttattt cgcaccacct
     5461 aagcggcggg ggcggttgga gcgattggtg cgaaataaga accattccct tagttactag
     5521 gttttttgtc cttatttcac acattctcac ttttcggtat aaatgattat tgcctttttt
     5581 tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg atttcttctg
     5641 tatcgtcgta agtataggtg tctaaaaaat cagttaaagt cgtgtaccag tcgtcgcctg
     5701 ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagcg agttcataat
     5761 ccattcccat taacacgata gattccgttc cacacgtcaa aaatctagta aatacaaggt
     5821 aataatcgtc tatcgtcgca tctttgctgt ttagctcttc catttcttct tccattaatt
     5881 ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc ctgcaaatcg
     5941 tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc atgatgtatt
     6001 tttctgtttc tagtcgtaga agctccgttc ctttttttac cgtttcagga cgacgtttat
     6061 acacgtctaa acgattatgg ctgactgtga ccttcgtttg actcgtttca ctttccaaat
     6121 acaattgttc ttc
//
LOCUS       DASNNU010000120         6064 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.122, whole genome shotgun sequence.
ACCESSION   DASNNU010000120 DASNNU010000000
VERSION     DASNNU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.122"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(136..1098)
                     /locus_tag="U8116_002509"
     CDS             complement(136..1098)
                     /locus_tag="U8116_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN8435443.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="U8116_002510"
     CDS             complement(1100..2539)
                     /locus_tag="U8116_002510"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEN8435444.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="U8116_002511"
     CDS             2724..4670
                     /locus_tag="U8116_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEN8435445.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4934..5806
                     /locus_tag="U8116_002512"
     CDS             4934..5806
                     /locus_tag="U8116_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN8435446.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1917 a         1043 c         1071 g         2033 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt ttatttaatc ttttcatata tcaattgtat gaaaaattat tagaatttta
     4801 aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat gttactaaat
     4861 aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt ctaaaataaa
     4921 gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
     4981 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
     5041 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
     5101 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
     5161 tacctcgacc ccgaaattgg catggcaaaa ttttgatttc atcggtacca tgaaaaaaga
     5221 atttccgatc ccgatttctt ggacaacaga tgtcaatgcg gctgcctatg gtgagtatgt
     5281 cttcggcagt ggcaaaggat tgagcagtgt cgtttactat acgatcggta cgggtgttgg
     5341 cggcggtgcc ttacaagaag ggcgctttat cgaagggttt agtcatccag aaatggggca
     5401 tatgctcgtc gtccctcatc caaaagatga ctttgctggc agctgtccat ttcatggcaa
     5461 ctgtttagaa gggatggccg ctggacccgc catcgaaaaa cgcttaggga aaaaaggaca
     5521 agaggttgca gaagatgatc cttattggga aatcgaagcc tcctatattg ctcaatgtgc
     5581 ctacaataca accttgatgt tttctccaga tgtgatcatt tttggcggtg gtgtgatgaa
     5641 acaacgccat atgctgcaaa acgtccacga cgcatttgca aaattggtca atgggtatgt
     5701 cgaaacacct gagttaagca aatatatcgt gaccccagca ttagaagaca acgctggaac
     5761 gctcggttgt ttggcattgg ctcgagaagc ggtcttacat tcataaacga aaaaaggctc
     5821 ttcgacattt agtgttggta gagaaagata caactgaaaa tttagtcata accagttagg
     5881 cagagtgctt aactggtttt tgttttactt ggaagatcaa gccttgaatg agataaattc
     5941 ttgctcgaaa gttatggaaa tttcgatagc cgtaggccac ttgtattgtg caatagtttt
     6001 ttacaaaaaa aagggggtgc ggatattttt ttggaccttt ttaacaaata tatctttgct
     6061 tctt
//
LOCUS       DASNNU010000121         5765 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.124, whole genome shotgun sequence.
ACCESSION   DASNNU010000121 DASNNU010000000
VERSION     DASNNU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.124"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(178..1869)
                     /locus_tag="U8116_002513"
     CDS             complement(178..1869)
                     /locus_tag="U8116_002513"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEN8435447.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(2289..3236)
                     /gene="arcC"
                     /locus_tag="U8116_002514"
     CDS             complement(2289..3236)
                     /gene="arcC"
                     /locus_tag="U8116_002514"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEN8435448.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(3351..4370)
                     /gene="argF"
                     /locus_tag="U8116_002515"
     CDS             complement(3351..4370)
                     /gene="argF"
                     /locus_tag="U8116_002515"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEN8435449.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(4461..5690)
                     /gene="arcA"
                     /locus_tag="U8116_002516"
     CDS             complement(4461..5690)
                     /gene="arcA"
                     /locus_tag="U8116_002516"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEN8435450.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         1534 a         1238 c         1013 g         1980 t
ORIGIN      
        1 tgtcacagtc cccttatttg caaacaaaca gtgtttaaga gacaacctct ccactaaacg
       61 gtgttcattc agctgactct cggtatttgc tcaaaaaacg ataaaatttg aaaagctatt
      121 ttctcttttg tttctctaat actcggatca gaacgctgat ttcacattct ctttttctta
      181 catcttatct ggtgcatgaa gtcctagcaa acgaaggtct tctttcaata atacagtcac
      241 tgcataaact agagctaagc gtgcttcttt ttgttcatca tcagctaaaa tcttcgtatg
      301 agcatagtat ttgttgaatg cttgtgctaa tttgattgca tgtttcgcaa tcactgacgg
      361 ttcgtatttt tccccagcag acaagaccgt ttcagggtat ttttgaacta atttaaccac
      421 ttcccagctg gtatcgtcat tcaatgcata gtctgctgtt tctgaaggga caaatcctgc
      481 tttttccaac aggctgactg cacgagcatg tgtatattgc acataaggtc cagtttctcc
      541 ttcaaatcga acaacttctt ccaggttaaa gtcaaatgta ttcagtcgat cgtttttcaa
      601 atcgtggaaa atgactgccc caaccccaac ttgtttagct acagcatctt tgttttccaa
      661 atcagggttt ttctcgctga tttgttcttt agcagattgg attgcttcat ttaatacttc
      721 ttctaataag acgatctttc ctttacgagt tgataatttt ttaccacctt gcgtgatcaa
      781 accaaatgga atatggtgca tgtcgtcaga ccagtcaaag cccatttcct tcaagacagc
      841 tttcaactgt ttaaaatgat agctttgttc attccctact acatatagag attgtttgaa
      901 gtcgtaagta cgtttacggt acaatgcagc agctaagtca cgtgtaatgt atagtgtcgc
      961 accgtctgac ttcttaatca acgctggatt caggtcataa gcagataggt caacgatctc
     1021 tgcaccttta tcctcgttca gcaaatgttt ttcttctaaa agtttgacga tttcatccat
     1081 cttgtcattg tagaaagctt caccgttcaa cgaatcgaaa cgtacttcca gcagatcata
     1141 gattttattg aattctttca tcgattcatc acggaaccat tgccataatt gaatagcctc
     1201 tttatcgcct tcttccagac gtttgaacca agcacgagct tcttcattta attctggttc
     1261 tgtttcagct acttcatgga attggacgta taaacgaagc aattcattga ttggttctgc
     1321 tttaacagct tcttctgtac cccatttttt gtaagcgacg atcagttttc caaactgtgt
     1381 tccccagtca cctaaatggt tgatgcggat tggctgataa ccgatttttt ccataatgaa
     1441 accgatcgaa ttaccgataa ctgtcgaacg caaatgtccc attgagattg gtttggcaat
     1501 attaggagaa gacatgtcga ttggtactgt tccttgattc ccgatgttgc tatccccata
     1561 atgttctttt tctttcacta ctgtttgcaa aacttttttg ctgataagtt ctttattcat
     1621 gaaaaagttc aagtaaggtc cgacaacttc gattttttca aagttggcag aatcgatttt
     1681 ttctgctaaa tctgcagcga tttgctgtgg cgcttttcgg taaacttttg ctaatgaaaa
     1741 ggctggaaat gcaacatctc cgtgttcagc tgattttgga ttttctagta attgctcgac
     1801 ttgttccagt gttaaatcat ctttgacagc gtcatagaca gcttttgcga caagatcttt
     1861 attattcatc tttattcctc ctaaagtgtt cccttgattg ttaaacaaaa aaaatctcta
     1921 gcgacggcat acttagtatg ccatcactag agacgagatt tcccgcggta ccactccaat
     1981 tgtcctaaaa aaggaccagc tccatctgat atcggtagac gaccgtctgt ttaaaacaga
     2041 attctccaag tgcgcttcgt tgtatcttct tcattcggct tccaccactt ccgaattcgc
     2101 tagccaagaa tgatacaagt actctcttgt tcaacaaatc ttagtcttat taaactttct
     2161 tgaattatat cagaaaaaaa aacgaaaggc tagtcgtttc ataaaaaaaa aatccagatg
     2221 gcctaaagga gaagaaatac cacctggata gctataaatc atttcgtaaa tacgaaagac
     2281 ttaacaaatt aatcagctac gataatcgtt ccttcttcag aagctaaaag attctcgata
     2341 ttttctaaag aagtgatgat tgccttagcg tttggttttg cttcaacaaa tgcaatagct
     2401 gcttctactt tcggaagcat gcttcctgga gcgaattgct tttcatcaat gtattgtttc
     2461 atttctgaaa cagtaacagt ttctaatttc ttttgatctg gtttttggta attgacatac
     2521 acattgtcta ctcctgtcaa tacgatcagc aaatccgcat cgatgatttc ggctaatttt
     2581 tctgaagcaa aatctttatc gattacggct tctcgtcctt ctaatccagt agctgtttct
     2641 acaacaggaa tcccaccgcc accaacagag actgtgataa ctccttgatc gaccagcgtt
     2701 tcgatgacac gtgcttcttt aatcgagatg ggtttagggg aagcgactac ttttctccat
     2761 ccacgtccag catcttcttt gaatgtcaca gtggagtcag cattcatttg ttctttggct
     2821 tcttcttccg tgtagaatgg accgactggt ttacttggat ttttgaatga aggatcattt
     2881 tcatccacaa taacttgagt gactaaagaa accacatctt tatcgatccc tttttccttc
     2941 agtacttcgc ccatggcgtt ttgtaaccag taaccgatag atccttctgt cattgcaaca
     3001 catgtatcta aaggcattgc cggtgttttc tcagaatcag cagcttgctg ctgaatcaat
     3061 aggttaccga cttgcggacc atttccgtgg gagatgatca attcatctcc ttgttcgata
     3121 aatttcacta aatactttgc tgtttccatt aacgcttctt gttgggcttt tgcacttgca
     3181 tccgtagaca agatggcatt gccccctaaa gcgacaacta cttttcgttt ggccatttca
     3241 ttcgtccttt cttcagttct accgaatttt ctagataaca aaacgagcca gcgttttgtg
     3301 attttccctc aatgctgact cgttttttcg tgagatgaat gtggttatgc ttaaacgcga
     3361 gggataaaca agttacctaa tgttgctgcc atgatcgctt tgattgagtg catacgattt
     3421 tctgcttggt caaattgacg agcatatttg ctgcggaaga cttcatctgt tacttccatt
     3481 tcagtgatgc caaagcgttc tttcatttgt tcaccgtaaa cagtgtttgt atcatggaaa
     3541 gctggcaagc agtgcaagaa gatcaaacga tctgtgttgt gtgttttttc taccatttcc
     3601 atattgattt ggtaaggttt caacaatttg atacgttctt cgaatttatc ttcttcaccc
     3661 attgataccc atacatcaga ataaaggacg tctgctccgt ctacaccttt atcaacatcg
     3721 tcggtaatca tcaattgtgc accggatttt tcagcaaagc cttctgccat tttgacgatt
     3781 tcttcttcag gttgtaattc ttttggtgca acgatacgca tattcgctcc taaaattgca
     3841 cctgtgacta ataaagagtt agccatattg tttcgaccat ctccgcagta agcaacagtg
     3901 ataccttcta ctgttccaaa gttttcttgg atagttaaaa agtcagcgat catttgtgtt
     3961 ggatgccatt catcagtcaa gccattccaa actggaacac cagagaattc tgctagttcc
     4021 tctaccattt tttgactgaa tccacggaat tcgattccgt caaacatacg tcctagtact
     4081 ttagctgtat cttctgtgga ttctttttta cctaattgga tatcatttgc acctagatat
     4141 tcaggatgtg cacctagatc gatcgctgca gtagtaaatg cagaacgagt acgtgtagat
     4201 gttttttcaa ataaaagcgc aatatttttt ccttctagat agtgatgtgg gataccacgt
     4261 tttttcaagt ctttcaagtg ttctgaaaaa tcgatcaaat attgtaattc ttcttttgta
     4321 aagtcttttt ctgctaataa gcttcttcct tggaatactg attctttcat attaaacatt
     4381 ctcctttaga ttgtttttat ttaagattaa agaggaaatc ccgacggcag ctgacagtaa
     4441 gactgccgtc aggaaacaaa ttacttcaag tcttcacgga caagtggttg gctcatgcag
     4501 cgtggaccac cacgacctcg tgacaattca cttgaattga tttctaacac tttgatgcca
     4561 tagctacgaa gcaattcgtt cgatacataa tttcggttat aagtgactac aacacctgga
     4621 gcgattgcca atgtgtttga tccatcattc cattgttctc gaggtgcaac gatctcatct
     4681 ccattaccag ttgggatcag tacaagatca tctaatccaa gagcagcttt catcgtttcg
     4741 tgtaagttat ctgaatgagt aatcttgata tcatcaccat ctggttcgat tgtaaagaca
     4801 tccatcttac cgtctttact ttgaattgct ggatggatag tgaatttatc gtgatctacc
     4861 attgtaaata cggtatccaa atgcatcatt gcacggttgt tcggaatttt gattgccaac
     4921 actttttcaa atcctgaatt ttttgcgaat aggtggcgtg ctaatttttc taaagctttc
     4981 gcattagttc gttgagaaat cccaacagct actactttat cgcttaagat caattcgtct
     5041 ccgccttcga tatgatctgg attttcacga tccaaccata cttcgattcc tttatttgca
     5101 aatcttggat gatgtttcaa aatatattct gtaaacatcg attcacgacg acgtgcttca
     5161 aaagtcatct tattgactgt catcccattt cccattgatg cggaagggtc gcgtgtaaag
     5221 tacaagtttg gcattgggtc catgtaaaat ggatagtcat cgtctgctga cagatcatat
     5281 agactgctag aacgtacttc gatatctttt gtacgaaccc cagccatgat tttatctacc
     5341 atttcttgcg tttccattga tagaaggaac tcatgaagac cctctcttac agcattcgat
     5401 gcaatatgag attcatccaa cattttattc aagaattgtt ctttgacatt gcctgcgtca
     5461 atcgcttctg ccgctaattt ttccaagtaa agagtctcga cgccttcatt ttgcaatgtc
     5521 ttcgcaaagt tgtcatgttc ttcttgagca atcggaagat atggaatatc atcgaacaac
     5581 agacgatcca taatatctgg tgtcaaattt tccacttctt gtccaggccg tttcagtaaa
     5641 actgtcttta atttccctat ttcagagaaa acgtgaatag gtttatccat gttgtgttgc
     5701 ctccttcttt ttgattacat gtataggata tcctcctttt gtaatcactt tcataatttt
     5761 gtctc
//
LOCUS       DASNNU010000122         5692 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.125, whole genome shotgun sequence.
ACCESSION   DASNNU010000122 DASNNU010000000
VERSION     DASNNU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.125"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(491..1300)
                     /locus_tag="U8116_002517"
     CDS             complement(491..1300)
                     /locus_tag="U8116_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN8435451.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1300..2046)
                     /locus_tag="U8116_002518"
     CDS             complement(1300..2046)
                     /locus_tag="U8116_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN8435452.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2064..2531)
                     /locus_tag="U8116_002519"
     CDS             complement(2064..2531)
                     /locus_tag="U8116_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN8435453.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2531..2941)
                     /locus_tag="U8116_002520"
     CDS             complement(2531..2941)
                     /locus_tag="U8116_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN8435454.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2952..3965)
                     /locus_tag="U8116_002521"
     CDS             complement(2952..3965)
                     /locus_tag="U8116_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN8435455.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4182..4916
                     /locus_tag="U8116_002522"
     CDS             4182..4916
                     /locus_tag="U8116_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN8435456.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4900..5610
                     /locus_tag="U8116_002523"
     CDS             4900..5610
                     /locus_tag="U8116_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN8435457.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1833 a         1195 c          943 g         1721 t
ORIGIN      
        1 ttaaagcgac aaaaaccacg agcaggtcca ttcatcctca aacctcggtg atcttcgcgg
       61 tttaacacca tcagaagaaa tgcacaaaaa taataaggag agtctgagac ataaataaat
      121 tcccagactt ttagtgagtc taaatccgaa taaacggtgg gtcgaaagca actctagctg
      181 cgcgtcgatc gcagcaatct ctaggtgcta tgttcggata aatggtggtc aaaagtcaga
      241 ttttcggcca tttcgtaaca gccaaccaga caaaaagcgt ctgttttgtc ttctactcca
      301 attgctcaaa tcttagcgac ttttggcttg gtcttactct aaacagtttg taagcctaaa
      361 ctttatttgc tgtttgtttc taagatcaaa gcaaaactac tttttagttg gttactttta
      421 caagttcttt ttactgttga ataaatgtaa atcttaagaa caaatattta cttctataat
      481 tccaagtttc ttaaaaaatt ccgccaagtg tacctaaaaa gcataaaaca aaaattccta
      541 gaataattgt gatcgtttta tgccctttgg agtaaagcca gacgcataaa aaggtaattc
      601 ctaatggcag caagcccgga aaaatttgat caaaaatact ttgtaatgaa gtttcagctc
      661 caccaaccgt gaattttagt gacgtattta aagtaaccat cgtgcctatc atcgcgccaa
      721 ttaccgttaa acctaaaatc gaagcaccat gagataattt gtcaatcata tggttgtctt
      781 ctgtatcatc aataaattta gttcctaaaa catagccata ttttgtcgcc aaccatttaa
      841 aaataaaatg aatggcagta tacaaagtaa aataaaggat catgcctaaa atattccctt
      901 gttgagcaaa agaaatacct atgccagccg caataacccg aaaagttccc cagaaaaatg
      961 aatcgccaat acctgataaa ggtcccatta atcctgtttt gattttatta attgcgtctg
     1021 tatcaaattc aggattttta gcattttctt cttccatggt gacggacaag ctcataacaa
     1081 aaggcgcaat atgcggtgta atgttaaatg tttccgtatg gcgctgcaac gctgtataat
     1141 aatcatcatc gttcggatat attttacgta aggaacgttc aataccattc atttgtccat
     1201 atgccatcat tttatcgaag gaccaagagg attgcataaa aaaggagcgc caaaatatac
     1261 ttcttaattc ttttttccca ataacacttt cttttaccat tataaatctt cctcctcttc
     1321 aaaagttagc ttttgaacag ccagtaacgc taagcaaagt ccgacgacag ccacaccgac
     1381 aacatccatt ttcagataca ttacgccgac aaatcctaag aaaagataag gcatcatcgc
     1441 tttattcgcc atcattttaa ttaataacgc tagcccaaca caagaaagca caccggccgc
     1501 aacatcaaag ctgtccaata caacctgagg aatcaacttg atcatcgcat tgattgtaga
     1561 cacaccaaaa taagtagcta caaaaactaa aatggccgat ggaaccccaa cgattaaagg
     1621 ttgagccaca taatgatatt tttgtgcttt tttgaaatca tgattgacta atgcctcatc
     1681 cattttgttg ttaaaatacg taattggcat tgcataggcg aagttacgcc acatctgaag
     1741 aaagatggac aaaggtaaag tcaacgcaac ggcagttcct actccttgac ccgattgaat
     1801 cgcaattgct gtacctaaaa cactggaagc ataaacttca gggggcgtcg cactccctac
     1861 cataattgag cccatgaaca tggattccaa ggtggcacca ataataattc ctgactctaa
     1921 atctcccaaa atcaaaccga caaaaggcgc tgtaaatagc ggtctaccca tcatcgatgc
     1981 accaaatgtg tattcatcaa tagacgccaa aaaagctacc agtgcgatta atattgcttg
     2041 tatcatattc ttcccctcct cttttagtca tgcaaaggat tgattttttt gtccgttggt
     2101 acagcctgac attgaactgt tatcccgttt tcagtcattt ttttcaccaa gtccagttct
     2161 tgttcattca agtaaacgga actagtaatc attttccgat tctcgttttt ctttaaattt
     2221 cctaaattca cttctttaat ctcaggtacg tgctgagtaa tttcgtaggc atcctccacc
     2281 gtcccgacaa ttaaaaatac ttggtatttc ttgatttttt ctccatttaa aacagagatg
     2341 gcctctgtca tatccttcac cacaaatttt gtccctgtag gaacagccat ttttagtgtt
     2401 tttttgcgca gattattggt agcgatttca ttacttgcaa cgaaaataca atttgcacct
     2461 aagttatttg tccaggcaaa agcaacttgc ccatgaacaa aacgttcatc tactcgtaat
     2521 aatttaatca ttaaaaatca tcctcttctg ttttctctag caactcgttc accaaaacta
     2581 tcccttcatt cccgctattc atagcttttt ggattagttt tttcgtgtcc tgttcacctg
     2641 ataagcaaag ctctaaaagc agcggcagat ttacaccgct gattaaataa aaattgtctt
     2701 taccgatttg ttcggagaaa gcgttagaca cgcttcctcc cagtaaatct accgtcacaa
     2761 ttaattcgtc atcaggcgct aagtcggaaa atagctgctc tatttcctgg ttaatggagt
     2821 agtcactatc tatatacgca gttattgcta aaagtgattt ttgaggacca ataataaatt
     2881 ctaaagtatc ctttaagcct ttagccatca tggaatgcgt cgcaattaca attttcctca
     2941 tagctcttac cttaatattc cacagtccga taataacgac gaatatctag tgaatgatcc
     3001 cgcacctttt caaaatgagc actcactcgt tctaaggccg ttgccataac cattggcgct
     3061 acatactgac gcatagcgct tggtacacct gctaattcga aatccgcagt gtcaaaaacc
     3121 gtcacaacat cgctatattt atttacaaaa cgctctacgc gatcgactag cggacgcgtt
     3181 tgatcttcac cttttaataa cacaaaactc atagatttat cagtcatttc aatagtccca
     3241 tggaaaaatt cggctgcatg gatcgacttg gtggcaatcc attgcatttc ttccagtacg
     3301 cacatagcat attcataggc agccccataa gtattcccgg ctccaacaaa catatgaaat
     3361 tccgtatttt tatgtttttc tgcaaattca agtgctttct gatcattttg ttgccgaaca
     3421 cttagtaata cttccggcat tttttgtaat gtttgaataa attcatcata ttcaggaaac
     3481 tcaccgttat ttttcatcag gcgtgcaccc aaagcaaaaa actgtaactg aagttcttct
     3541 actaatgtat cgttgtcagc atcattttca atcgcataat ccacgatttc cccgagtggt
     3601 gtagcagcat ttcctacaaa cgcaatcgtc gttgcacctt gactattggc atacttaaca
     3661 acatcaacgg tttcttgagt cgtaccggtt aatgaagatg tgattaacac tgtatcttta
     3721 ttaatctttt tcggctgtga acaaaccacc tctgcggcta tttcatgata ataatcaagc
     3781 tctgaatgag tgcttaacag ataagctagc gggctgattg tggcatatgt tccaccagag
     3841 cctaccaaat aaagattttt aaatcccttc tcactgattg catctgccac ctgattgatt
     3901 gtctcgatat tttttactgc accatttaat actgatagaa aacgttcttc gctaaaacct
     3961 aacatagtta aaaacctcca agtcaataat ttgaaaaata tatttgcaaa tgccggcaca
     4021 ttaacaaacc acttaacctt ttttatatac cacctgttaa cgtttttaat gtaccactta
     4081 tagaaaacgc tgtcaatagt ttttaaccac ttttattttt atttgtacca aaatcctttt
     4141 ttcatgtata ataaacaaaa atagactctg gagtgtaacg tatgaatact gaagaactaa
     4201 cgaacaaatt aatacaagac attgaacaag aaaaatataa atacgcgcaa gctattcctt
     4261 cagaacgtga ttttgttcaa atttatgaaa tgaatcgttc agccgtaaaa aaagccattc
     4321 aacaattagt aaataaagag tacctactaa aaatacatgg caaaggaact tttgttaaaa
     4381 aaaatatcca tgaacatttg caaatcaaat ttcatggttt gtctgaattg ttggaacggg
     4441 aagggattca gcctagcagc ttaattttag cttcagaaat aaaaccggct ggacacattg
     4501 tcgggaaagc tttacagctg gaaccaactg aagaagtatt tcatttggtt cgtttaagaa
     4561 gcggcgacgg tgagccaatt tctattgaag acacttatat tccattaaat ctcatacctg
     4621 atcttcaagc ttttgatttc caaatttttt cattttattc cacattggct gcttatcatt
     4681 atcaaattga aaatatctat cagacaattt ccagctcaaa agtgagcaat aaagaagccc
     4741 gtttgttaaa aaccagctta aatcgtccgg taatcagcct tgctatcact gcggttacga
     4801 caaatgaaca agcggttgaa tacactgaag tagtggtttt gcctgaattc gccgccatct
     4861 atactaaaaa tgttcatcaa ggaaaggaga tgaaaatcta tgcccaaatt gattaatctg
     4921 ttggcttatg acctgattga caaaattatt gcttatattc atgaacatca tttaaccccc
     4981 ggaacaaaac tgccttcgga acgggaccta tcagaaatgt ttaatgttaa tcgaactacc
     5041 ttaagaggcg cgttacaaaa actggtggac aacggcacct tacgggcgca aaccaatagc
     5101 ggctattatt taactgcgcc aaaattcgaa aaaaatgccc atcatttctt tacaccgata
     5161 gaagattctc tgcttagtaa gcagcggcta cagctaaagg caacggctct ttcaccgaaa
     5221 aaagcagcag ctctttgtcc ggaactgaat gatttcaaac agcagcaacc acaagcttat
     5281 ttagaatata ttgatgcaga agcaattagc ttgatgttat taatccctat ggaaacatta
     5341 aagaaaagta atttctcgga caatcctttc caagatttta aaagtttggc atatacgcgt
     5401 tctcaagaaa tttctttaat aactgacaat gacgttgcgg cggacttgct gggagttgac
     5461 aaagaagagg ttctgttatt tgtacgagag gaaataagag ccggtaagga gttggcggcg
     5521 attgctttag gttatactat tagtcgccgc tgcaacatac aggttaatgt tgctacgacc
     5581 ttgcaatccg gttctgttgc aaagttttaa atctactatc agataaggta gaataataga
     5641 aaaagatagc aggaggaatg acgatgagtc attttaaagg aaagcaattt ca
//
LOCUS       DASNNU010000123         5572 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.126, whole genome shotgun sequence.
ACCESSION   DASNNU010000123 DASNNU010000000
VERSION     DASNNU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.126"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..197)
                     /locus_tag="U8116_002524"
     CDS             complement(<1..197)
                     /locus_tag="U8116_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435458.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKE"
     gene            complement(199..480)
                     /locus_tag="U8116_002525"
     CDS             complement(199..480)
                     /locus_tag="U8116_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435459.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            complement(856..1380)
                     /locus_tag="U8116_002526"
     CDS             complement(856..1380)
                     /locus_tag="U8116_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN8435460.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            complement(1397..2365)
                     /locus_tag="U8116_002527"
     CDS             complement(1397..2365)
                     /locus_tag="U8116_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN8435461.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            complement(3532..3687)
                     /locus_tag="U8116_002528"
     CDS             complement(3532..3687)
                     /locus_tag="U8116_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435462.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(3706..4275)
                     /locus_tag="U8116_002529"
     CDS             complement(3706..4275)
                     /locus_tag="U8116_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN8435463.1"
                     /translation="MTIKWIISIAYLVLTFAIPSLGVPSNIYFLFEHSDIFFLVLIII
                     LFHSTFIREFKEVNLKKLFKYSFFSFSVLFLINILNTSFSEGINPNEVNGSLLLFFLN
                     AATYGAFLEEGIFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFV
                     YIQTKNIWYSIVAHGFYNTIGILIYLISI"
     gene            complement(4275..4730)
                     /locus_tag="U8116_002530"
     CDS             complement(4275..4730)
                     /locus_tag="U8116_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435464.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(5350..>5572)
                     /locus_tag="U8116_002531"
     CDS             complement(5350..>5572)
                     /locus_tag="U8116_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435465.1"
                     /translation="ISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIENQLNPS
                     RRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1676 a          907 c          942 g         2047 t
ORIGIN      
        1 atttcttttc ccatggcatt gagttttgct tggttactgg ttgtggttgt attggtatca
       61 ggttcataac tcatacataa aaaacgaatg gcttctgaat aattgttgac gtctggattt
      121 ttttcgatga tggaatctaa aacgtcaacg tgactgggat aaagggttac gtgtttttga
      181 acagaagttt ttctcatgtt atattcctcc gataagtgtt tcaatgagtt ggttgtttgt
      241 gtgaacatag tcattcagta tgttcgtatt ttctgtttga atggcaataa atttttcgag
      301 taataaatcg aattgatctt ttttgtaatt cataagttga cggtcaaaat cactttcgat
      361 tagaactttt agatatttat ttcgactcca ttttttgccc gttttggttg tgagttcctg
      421 acaacgttta tctattcttg caataatttt gggattgata tttcgaacta aaatgtccat
      481 ggaaattttc ctttcgtatc agcgtataat ttatgtatac catacaaata tagtatttgt
      541 atagctattt ttcgccaaaa cactatacat agcctagcca caatggcaaa aaagtgatag
      601 caaatttttt tctgagaaac gtgataggca cagcctatct agcacctttc gtaagaaagt
      661 gataccacgt ttccttatgc tctttcaaaa ttcagaaaaa tcagctgtaa aacgagtgca
      721 gtccgagtaa gattcaacga aaaaacgggg attggggcgc caccaaaata tcgcaacatg
      781 ggtccatgtt gctctgctcg caaaagcaaa aagaagaata aaaccagcaa tttttattaa
      841 tttaaattgc tggttttatt gccagaattg ccaccatttt ttctccgcct ttgattgttt
      901 ttctatcgat tctgtttctt cagaaagagt cgttgattta ttttccgaag attcttccgt
      961 ataagtcaac tgtagttgtt cctgcagttt ctcgatttgt tggttggctt gcagggtgag
     1021 ttgttgttgg tggtcgagta atttttgcat ttgttttatt tgctcgtctt tttcttgtag
     1081 ttgcatatct ttttgattta actgttgttc taggagagaa aggacttcta attcgggaac
     1141 ttggttggcg tttttgtggt tttcggtttt cgttttttcg ccaactagtt gttgatttga
     1201 gttttcgttt tcgttaaaaa acattgattt tatagccttt tgtccggttt cattaatcac
     1261 aaacttgttt ccgattttcg aaaaccaaag ttttcgattt tcgtcagaaa cttttttgtt
     1321 aattgctgtt ttactaactc ctaattcttc agcaagttct ttgattgttt tttctgtcat
     1381 aaaatctaaa aacccctcaa tctttgaata agttttgaag attgtctaat aagtcttttt
     1441 ttagttctga aggaatatcg gaatttatct ctgatttttg tgcattattt tctttatttt
     1501 gggtattttg ttctctttct aacttttcta actgttgtaa aagttccaat tttcgctttt
     1561 gttctttgat tttttcttgt aatgaagtag gtgctttttc agattgaact tgttggttag
     1621 ctagttctaa tgctttgttt tgttcttctt ttgttggggc atgtttgtta aagtcgtctg
     1681 atttttttat ctcaggttta aatgtaaatt gatagccaat gacaggcttt ccccgtcctt
     1741 ttccgtattt ctttttgatt gttaaacctt taaagattgc ggttagttct tgtcgtattg
     1801 gattgaacac acgttgatca atcgtacctt ggttgtaact tttaggaata tcaagtaatt
     1861 catgaaagtc ttcttttgaa aaataagcga aacctttggt tctgtattgt tttagtaatc
     1921 gaaacattgt ttttgaataa ctgctttcta attcattgaa ttgttggaga gaatagcgca
     1981 cccattcttc aagattatta agtaagggta aggctttttc atgaacttgt atttcagcaa
     2041 aaggaatatc agattttccg tcgattttaa attgattaaa taaaacaaaa cgttcacgtt
     2101 gcaaaccgtc agcacttctt ctaccaaaac gtaaatccat taatttgtca tacgttgttt
     2161 ctaaatcatc tatgaatcga acatttgcag ttgctttata attgcttaaa tcttttaatt
     2221 gctcgaatgt aaattgtact tttttatcac ctacatcacg cattcttgat acgatagaaa
     2281 agaacaaatt catttcgact ggtgtaaatt ttcgcaacgg aattgtattt aattcatgat
     2341 gatactttac gatttcatta gacatattta aaatagctcc tttcttactc gcacaagaga
     2401 tacacgttat gaaatacaaa atgcctgttt atctacatct tatattaaca tcgtcgatgt
     2461 actaattcaa tgtacaaaca gacaaaacga tgtagataaa cagacaaaac gatgtagata
     2521 aacagacaaa acgatgtaga taaacagaca aaacgatgta gataaaagcg tcattccctt
     2581 gggagagtaa ggatatagcc gacggtaaag aggtttaaaa agaggtttaa aaagagatta
     2641 taaaagaggg ggacaaaatt tcccgcctaa aaaaccactt cttttttctc caaacagatt
     2701 cgcgcatttt tctcaactgg gaatcttact ccctacagtc gcttaaaaag gtgtgatcgc
     2761 tctgctctca cattatactg cgcagtcgct tccggctcct ttgctaaaaa tgttcataag
     2821 gaactctttg agaagctatg agccttcgtt tgagacgttt taagacagtc ttagtataaa
     2881 tcccttatct tgttcttaaa gcgccttaca ggcggtcagg gcaagcccta acaaccctaa
     2941 agttgtctga atgccctcta aaattcgatt taagcactct taaacctact cttatatatt
     3001 tatatactta gacgtttcaa acgtcttaaa acgcaaatta gagcttgata aagatatttg
     3061 ctcgtgtgcc gactttttta aaagtctagg cacacgccat gacaaagctg aaataaactg
     3121 cttcagcttt gtcatggcgg attcgctcac ttgaaaaagt gttcatttcg ctcgattatt
     3181 agcatatatt ttttaacttt ttttgctagc caaattttgc attagttcgc tcataaatcc
     3241 caccctttca gtacgtccta cggacgtatc gttcgtaatg tgaaagtacc tttaaaggta
     3301 ctttctacaa aactcactaa agtgtttggc aagctctata tttgctagcc aaatacctac
     3361 gtatttggtg caaataaccg cttgtcaaag gggcgcaagc tcccccttga atcccccctc
     3421 aaaacccacg ctccacaata caaaaaaggc tagcaaatgc tagccacttt ttgtaaagtt
     3481 tcgcttgttt caccttcggt gaaattgttg ctttgcaacg atcaggtaga gctacatttt
     3541 ttttcgttgt tcaaatattc ttacgccatt tactattaaa atcgtaggta ttccaaagta
     3601 aagaaaaaat tgattagcag gtaatacaaa atataaaata ataccagcaa taattaccaa
     3661 acttatagta agttttgtta atttcatgaa gtatgccacc ttatcttata tagatatcaa
     3721 atagattaat atgccgattg tattataaaa tccatgagca actatggaat accatatatt
     3781 ttttgtttgt atataaacaa aacttaaaat aataccaata aagaaaatac ttaggcctcc
     3841 cccatgaaca atagaaaaca gaaaagaaga aattagtatt gaaatatatt gttgtttttt
     3901 gtttgcttga ggatcaatca tacaaaatcg aaaaattcct tcttctaaaa aagctccata
     3961 agtagcagca tttaaaaaga ataaaaggag agaaccattt acttcattag gatttatacc
     4021 ttctgaaaaa gatgtattta gaatattgat taggaataaa acagagaatg aaaaaaaact
     4081 atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg tagaatgaaa
     4141 tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa aatatatatt
     4201 tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa tactaattat
     4261 ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc tgtagaagtt
     4321 tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac agttattctt
     4381 gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc tatttcgatt
     4441 ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg agtttctata
     4501 cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga ccaagtcctt
     4561 gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata agtggctata
     4621 tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc agaaccaaaa
     4681 gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat caaaatcacc
     4741 tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat caggaaattt
     4801 aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag tagacataat
     4861 gtacactatg ccaagtagcc cctctaccaa tggatagaga cgctcacttt gtgattttta
     4921 attggtctag ctagttacta gcttctaatg cttgatttta cgcgatttct cttttttagt
     4981 tctttgtgca tttttatctg tcatttttta aagacttgtt atttttgaga ggatttgata
     5041 tgcttttttt gtctttctct agttcttcac tttgtaatat ttttatgttg atgaatagga
     5101 taatctcatt ttgggctgct cgtgagagca gtcttttttt gtttcagggt acgtcctatt
     5161 ctttggattt ccgcgctatt aaataaccga tacttgggga ttggggtagc gccccaaaat
     5221 acaaaaagaa gaagttgcat aagcggattt ttcttttttt tcgcaagttg gtaccaactt
     5281 gcaaatcttg ctggaagact gatccctaaa aagaaatcga ggaatttttt tagggatcgg
     5341 tttctttttt tatcttttta gaaagtttgt tgggtcaaaa tttccagaag taaaggtttg
     5401 attggtttct ttttgacgat ttttgggttg atttcgtcgg gaaggattga gttgattttc
     5461 aattttttct ttcacttctt gatacacttt agaatttgta ccgataagaa caccagtatc
     5521 ttttagatat aaatcagcaa attctgccat gatttctgtc atgatagaaa tt
//
LOCUS       DASNNU010000124         5550 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.127, whole genome shotgun sequence.
ACCESSION   DASNNU010000124 DASNNU010000000
VERSION     DASNNU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.127"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="U8116_002532"
     CDS             131..505
                     /locus_tag="U8116_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN8435466.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="U8116_002533"
     CDS             complement(619..1248)
                     /locus_tag="U8116_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN8435467.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="U8116_002534"
     tRNA            complement(1382..1454)
                     /locus_tag="U8116_002534"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="U8116_002535"
     tRNA            1668..1741
                     /locus_tag="U8116_002535"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="U8116_002536"
     CDS             complement(1879..2043)
                     /locus_tag="U8116_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435468.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="U8116_002537"
     CDS             2338..3666
                     /locus_tag="U8116_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEN8435469.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="U8116_002538"
     CDS             3868..4701
                     /locus_tag="U8116_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEN8435470.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="U8116_002539"
     CDS             4717..5454
                     /locus_tag="U8116_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEN8435471.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
BASE COUNT         1878 a          849 c         1094 g         1729 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc
//
LOCUS       DASNNU010000125         5522 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.128, whole genome shotgun sequence.
ACCESSION   DASNNU010000125 DASNNU010000000
VERSION     DASNNU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.128"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..311
                     /locus_tag="U8116_002540"
     CDS             <1..311
                     /locus_tag="U8116_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435472.1"
                     /translation="DIEDSNTNITIQDYKKVYDNPISCALSQGQIDGLAVIEDGEMVN
                     VSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFATKPFYYPSHCEVNLIGSEKNG
                     "
     gene            304..699
                     /locus_tag="U8116_002541"
     CDS             304..699
                     /locus_tag="U8116_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN8435473.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            701..1078
                     /locus_tag="U8116_002542"
     CDS             701..1078
                     /locus_tag="U8116_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435474.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            1079..1687
                     /locus_tag="U8116_002543"
     CDS             1079..1687
                     /locus_tag="U8116_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN8435475.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            1687..2028
                     /locus_tag="U8116_002544"
     CDS             1687..2028
                     /locus_tag="U8116_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435476.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            2270..5059
                     /locus_tag="U8116_002545"
     CDS             2270..5059
                     /locus_tag="U8116_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEN8435477.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            5049..>5522
                     /locus_tag="U8116_002546"
     CDS             5049..>5522
                     /locus_tag="U8116_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN8435478.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSS"
BASE COUNT         1994 a          708 c         1099 g         1721 t
ORIGIN      
        1 cggacattga agattctaat acaaatatta ctattcaaga ttacaaaaaa gtatatgata
       61 atcctatttc ttgcgctctt tcacaaggtc aaatcgatgg actagcagtc atagaagatg
      121 gagagatggt aaatgtttca actgacacat ataaattatt tgttcatcct aagattaaac
      181 taaaaaaagg agatcgaata acaataactc aaaaggccag tggcttaatt ttttctctat
      241 ttgctactaa gcctttttac tatcctagtc attgtgaagt gaatttgata ggaagtgaga
      301 aaaatgggta atctcaaata tgaatcaaat gcagaaaaga tcattgagaa ttttaaaaat
      361 atgactgtaa ttgctcaaaa agaaggaata tccttcgtta acgattcaat gaataaagtc
      421 gttagtttag ctaaaccatt aactcctgta aaatcaggta atttaaggcg aggatatcgt
      481 gtagtaaaag ctagaaaact atcaagcggt cgaattgttg gagaagttat aaacaatgaa
      541 cattatttta aatatgtaga agaaggtcat aggactaaaa atggtggctt tgtcaaaggg
      601 aaattcatgt taactcgtgc aacgaatctt gcaaatatgt cttatattcc tcgaagattt
      661 aaacaaatgg caataaaaat cgttaagaaa ggaaagtaac atgaaagatg aaatcattgc
      721 tgcaatcagt agcaaattaa aagaaatcta tcctgatggg acaatatatc ttgattcagt
      781 tatgcagtca actaaagatt tttatttcgt tttatcagta atggaatctg gaactgaaaa
      841 tgtaggaatt gatattcaaa atgtttcttt cttaattgat attgcattga ttgataataa
      901 ccctaataga aatttaatta atgaattagt ctcacgttgt gggacttttt ttaatacgat
      961 tacaatcgat gagcaaatat tatttccaaa agcctattta cctgatgaag cagatggtgt
     1021 tcaacatatt cgttttacat tagaatttcc acaatatatt gaatggagtg aaagataaat
     1081 gggagaaaaa agaagtaaaa ccggaattat ttctgtcgaa aaacctactt ggtttccttt
     1141 ggaagatgag acagggaatt ttccggtata tggtactgcg ttgccaatgg gaacagcagt
     1201 aagtatcaaa ccaacagcta attatgaaac aacacaagat tatggggatt cagtggttca
     1261 agatcaattt acagcttttg gtggtgcaga agttgaactg gaagcaaatg gatatactca
     1321 taaagtttta tctacaatta ctggagggaa aattgttaaa ggtggtgcct tgcgttctgg
     1381 agaagatatt gcgcaagatg gtgcgtttgc atatagacgt aaaaaatcga atgggaaata
     1441 ccgttatacg gtcttttata aaggacagtt tgctctagat tcagatgaaa catcaacgat
     1501 tgaaggaagt aaagtaagtt ttactcatcc aacttggaag ggttcatttg tagatgtacc
     1561 aggacttgga tatatgtatt cagttgatga agatgatgaa ggcgtggata aagcaatgat
     1621 cggaaattgg tttacaaaag tagcaattcc aattgaagaa gcagaacttt caggaggtac
     1681 agaataatgt caaaatatca aacaacgatt aaattaacga aaaaagacga agaaggtaaa
     1741 tatgagcaag tacaatttaa atctgctgaa tttttaccag gaacagttgt agaagatgct
     1801 gcaggagtta tggaagaaat gcaaacagcg actgataagc aatcagttaa aaaagcttta
     1861 agtcgtgctt attcatttat tgcagatact ttatttgagg gacaatttac tggtgaagat
     1921 tattgcaaag gaattgatgc tcgtgagatt gcctcattga caggaaaatt attgaagtct
     1981 gttactgcag gttttgatga aacttataca gagacgaaaa aaaagtaagt gaggcgctca
     2041 agtcaccttc atttaagtat tcgattactt accgagaatt agatataaag acgcaattac
     2101 tcgaagcagg ttggacgtta ccagaaattg aacgtactga cttggatgag ttaatgcgtc
     2161 tttttgcttt tagagatgcg gtaaaagagc atgaagatgt tgagtactat gataatttca
     2221 ctcaatttta ggaggtgata ctttgaataa cgatgaccta attctgaaga tgatactaga
     2281 tgaatctggt tttactgccg gtatgaataa tgctgtcaaa aagttgaact cttttgatga
     2341 aacaatcgaa agaaaaagta gaaatagtgg cagttcatta ggtagtattt ggaaaatatt
     2401 tgctggaagt tttctagcta gcggagtaac tagaatcgta ggagctggct ttgatctaat
     2461 caagggttcc ataagtggag cggttgatcg agtagatacg atgaataacg ctctacgaaa
     2521 tttccaaaat atgggattta gtaattcaga aattatgaag aatatcggaa agaatgggct
     2581 tttatctcaa ggtattcaag gacttcctac tgctttgaat gatgcgataa gtcatgttca
     2641 acttcttgct tcttctacag gtgatttaac tcgttcaact caaatattta aagctttaaa
     2701 tgatggaatt cttggtttcg gtggttcaac tgatcaagtt aatgaagctg ttattcaatt
     2761 atctcaaagt ttctcaaacg gaaaagtaga cgcacaaact tggaactcaa tgatcaacgc
     2821 tcaacttgga cctactcttt ctgctattgc taaaaagatg ggaattacaa tgggagatct
     2881 gaaagaaggt ttatctcaag gtaagatttc tgttgaagaa ttccaaaatc aattaataga
     2941 aatggatacc aaaggtggcg gaggacttaa atcattaagt caaatcgcta aagattctac
     3001 taagggaatt aaaacctcta tacaaaatgc aaaaacagct gttacacgtg gtgttggtga
     3061 ggtaatagaa ggattaaata aagcattagt agattcagat ttaggtggat ttaagggaat
     3121 tattgataaa gtgggcagtt caatggaatc gttcttaaaa gtaattgctg caaatattcc
     3181 tacagcagta tcatttttaa gtaatttatt taacgaaatt caaaaatttg gttctgcttt
     3241 gaaattcatg atgccctttc ttgttcctgc agctaccgct tttggagcgt tcatgtttca
     3301 acttaaaggt ataccagcaa taataaaaag cttcaataat tttaagaatg ccataattgg
     3361 cgttggaaat tcattaaaga ttatgggggc aatagctgct gctaatccat ttgttttaat
     3421 tattggtgca gtcgttggtg caatagctgt tttcggttac tttatggcaa ccaatgaaga
     3481 gcttagaaac aaggttatat ctatttggaa tgatgtgaaa gattccgtaa ttggtgtatt
     3541 aaaaaatata aaggactggg gaattgatac ttggaattct gctaaagaaa tggcatcaaa
     3601 tgcagttgag ggtgtcaaag atgcttggtc aggaacaaag gaatggtttt ctaacacctg
     3661 gaaaggtatt aaggatgggg ccacaggttt atttgataaa acagtagaga cttcaagaaa
     3721 tgcagtcgat agtgtaaaaa atgcatggtc taatatgaag aaatggtttt ctgatacttg
     3781 gcaaagtata aaagattcag ccagagaaaa atggaatgga atcaaaggtt ctattatgtc
     3841 agttgctggt ccattgatta caggtataaa gaatgcattt ttacatgtta ctttttatct
     3901 agagacctta tggaataatt tagttgagat tggtaaaaac gtctttgaga ttttaaaaaa
     3961 tgttatactt gcgccggttt tgtttattac ctccatgatt tctggtggat gggaagaaac
     4021 aaaaaataat atgattgctg tttggaataa tattaaagaa agtgccataa atatttggga
     4081 atctataaag aatatatttg taagttattt tacaaatatt tattttgctg cactcaatat
     4141 ttggacaggg ttcaagctta cattgataaa tatttggaat gaagtggtaa atcaagctaa
     4201 atcgatttgg ttaaacataa agtatttttt tattaatctt tggattgata taaaatattt
     4261 tgcaattcag aaatggattg aattgaagtt ttctattatt caaacttgga tcgatttaaa
     4321 atataatgcc attactactt ggaataatat taaacagttt ttcaaagata cttggaaaaa
     4381 tattaaagat acggcataca atacgtggat ttctataaaa aataccatga ttaatacatg
     4441 gaataacatt aaggactctt tctggaatat tgttactgga attgttaatt ctgctgaaaa
     4501 tgcatggaca aatctaaaaa atggtgtttc aaatgctatt aatcgggtaa aagaaatctt
     4561 taattcgtta agggaagtca atttatttga aattggcaag aacatcattg atggacttat
     4621 aaatggtgta aaagaaaagt ggaatgcttt gaaagatact atcaaaggta ttgcaggtaa
     4681 tatcaaaaat tggattaaag gagctttaga tattcattct ccatcaagat ggatgcgtga
     4741 tatggttggt aaaaatattg ttcaaggtat tatcgttggt attgataaag agcaaagtaa
     4801 gttagatcaa accatgacaa atttagtaaa aacaccgtct gttcaacctg tagtaaatgg
     4861 aataagtcac ctaccaatta cacaaacaaa gcaaaatata tctgctagtg aaacaaaaga
     4921 aattcattta catttaaatg tttatggaga tttacctgat tcaatggttc ggaagattac
     4981 cacaaaaatt aaaacagaat tgacaagaca aatgaaacga gatgctggtg cagtaggagg
     5041 aacattatat gcaacttaag agaggacaat tttttattaa tcaacattat tcttctgaat
     5101 ttaatgtgta tattcaaaac aggcctgctt ctgtttcagc tagtcgagta attgaattga
     5161 gagagcgaga aggaaatgat tctatcatta ttgataaagg ctattacaaa aatgtgacta
     5221 gaaagattga gtgttactat aaagcacctt caattgatat ggtgcaggag tgggaagatc
     5281 gaatcactga atggttagat atgtcgtctt atagtgattt tattctttac tatgatgaac
     5341 aatatattta tcaagctgta gtaatagaag gacctgaatt taaaggaaca agaaaaacag
     5401 gaaatattgt cccttttgaa tttacagtga gtattcgacc atttaaggaa aattacaatg
     5461 gtagatttac tattcgacaa acagaaactt ttgaaattta taacccagag aagtattctt
     5521 ca
//
LOCUS       DASNNU010000126         5481 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.129, whole genome shotgun sequence.
ACCESSION   DASNNU010000126 DASNNU010000000
VERSION     DASNNU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.129"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="U8116_002547"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="U8116_002547"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="U8116_002548"
     CDS             complement(580..1353)
                     /locus_tag="U8116_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN8435479.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="U8116_002549"
     CDS             1497..1847
                     /locus_tag="U8116_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN8435480.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="U8116_002550"
     CDS             1828..2544
                     /locus_tag="U8116_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN8435481.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="U8116_002551"
     CDS             2544..3992
                     /locus_tag="U8116_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN8435482.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="U8116_002552"
     CDS             complement(4062..4571)
                     /locus_tag="U8116_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435483.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="U8116_002553"
     CDS             complement(4561..5286)
                     /locus_tag="U8116_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435484.1"
                     /translation="MKIIHYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          954 c         1095 g         1568 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agtggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DASNNU010000127         5455 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.130, whole genome shotgun sequence.
ACCESSION   DASNNU010000127 DASNNU010000000
VERSION     DASNNU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.130"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            48..1166
                     /locus_tag="U8116_002554"
     CDS             48..1166
                     /locus_tag="U8116_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435485.1"
                     /translation="MSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYV
                     NWEGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLK
                     EEDKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDF
                     EVVYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERI
                     YLLSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEI
                     FVVSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASY
                     QNGLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1387..3150
                     /locus_tag="U8116_002555"
     CDS             1387..3150
                     /locus_tag="U8116_002555"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEN8435486.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3234..3692
                     /locus_tag="U8116_002556"
     CDS             3234..3692
                     /locus_tag="U8116_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEN8435487.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3801..4484
                     /locus_tag="U8116_002557"
     CDS             3801..4484
                     /locus_tag="U8116_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN8435488.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4631..5245
                     /locus_tag="U8116_002558"
     CDS             4631..5245
                     /locus_tag="U8116_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEN8435489.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5261..>5455
                     /locus_tag="U8116_002559"
     CDS             5261..>5455
                     /locus_tag="U8116_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435490.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
BASE COUNT         1939 a          855 c         1121 g         1540 t
ORIGIN      
        1 atggtatatt cacgagtaca gaactggctg atcttgaatt tatttctttg agcaaagtat
       61 atacaatcaa aaaaatcgta ttggattttt ttaaagaaaa tgattatttg aaaaataaag
      121 aaatcattat cccagaattt gactcgcgga acatcttgct agctttagta cgttatgtca
      181 attgggaagg ctatgaaaat aaaaatccgt atgtagaaga agcttgtaac gagttgattg
      241 attatgtaga gactcatttt tttaaaagaa gatattcgga agatgaaaag tcctttatca
      301 tacgaggaat agagatcgca atcggaagaa aggcatttcc ccttggactg aaagaagaag
      361 ataagaaaag cgcagaaacg aagccgttat ttcaattagt ctgtcaagga ttggaagagc
      421 aaaaaggaat tctagactta caagaagaag atgtctatta tattttttct ttgttcaaca
      481 gtagaaatta taccaatgag aacatggagc tgttaagaaa agatttcgaa gttgtttata
      541 aaaattttgt tctcaatgac ctatcactga atgaattgat tttacagatt attagtatcg
      601 ctgaaatcga cgatgcagaa aattttattt tcaagcaatc ctttttacaa tttgtccgta
      661 cgttgtgggc agatggacaa gtatttttac cggaaaggat ctatcttcta tcagaaaaag
      721 aacaacttct gtacggaaaa attgtagaaa tattggaaga gtggaaaaag aaaaatcaaa
      781 tccaactaag atggaatgaa aatttcattc gaaaattcgt caaaagtctt tctttgatca
      841 acagtgaaca agcggggaga caggaaatag aaatttttgt tgtttcagaa agttcagtca
      901 aacaattttt ttatcgaacc caatttcatt tgtatgcgga agaaggcatc gctgaaataa
      961 atcctatttt atttcgttca ttagaagaat tgcctgatga atgtctatgt gcgaaaaaaa
     1021 gagtggtttt gtgcgatgct gctagctatc aaaatggatt agaaactgaa aagacaaaga
     1081 tctatccaat ctcattaaaa ggtgcaaaca tccatttgta tcatattgct caggaacttc
     1141 acctattaaa aaaacatatt tcttgatgag accttaatta ggagaccttc tacaaagctt
     1201 tttaattgtc tgtagaaggt ttttatttga gtcctgattc gtgcgaagat ctacttatat
     1261 aacaagcttt ctctgaaatt tttttaaaat gctattgact tagagtcaac tctaagtagt
     1321 agaatagata aagtgtaaca aatctatttt cattaaaaga agatagtacg gaggagagaa
     1381 tgtattatga gtacagtatt acatgtattg gatgcaatca atagccccgc agatgtcaaa
     1441 aaattatctg ttgaggaaat gaaacagttg gcagaagaga tgcgccaaaa aatcttgcaa
     1501 aaggacagcg atgtaggtgg gcacgttggt ccgaatctag gtgtagtaga attaacgatt
     1561 gcgtttcatt atgtatttga ttcaccaaag gataaaatcg tttgggatgt ttcccatcag
     1621 tcttatggtc ataagctttt aactggtcga aaaaatggtt ttgaaaaagg acattaccgt
     1681 gaagtgagcg gatattccaa ccaacatgaa agtaaacatg actattttac agtggggcac
     1741 acatctacat ctatcagctt agcaacaggt ttagctaaag cgagagatct gaagaaagaa
     1801 tcaggaaatg tggtagcatt tatcggtgac ggctccctaa gtggtggctt agcttttgaa
     1861 ggattgaaca atggtggtgt tttacaatcc aatctaatcg tattagtaaa cgataatgag
     1921 atgtctattg atgagaatca tggcagttta tataaaaact tagctgagct tcgtcaaacc
     1981 aacggtaaaa gcagcaataa cctatttaaa gcaatgggat ttgattatcg ttacgtggat
     2041 aatggaaacg atctggaaac ggtgatccgt ttatttgaag aagtaaaaga tattgatcat
     2101 ccgattgtac ttcacgtcca tacggaaaaa ggtcgcggtt atcagccggc aatcgataat
     2161 aaaatgaaat atcattggca tgtaccattt gatctgtcca ccggggaggc aaagcaagta
     2221 acttctggag aaacatttaa tatgatcatc atggattatt tggataagaa aatgcaaaca
     2281 agtacagctc ctattgccgc agtcaatgca gcgataccag gcgcctttgg actgaaaaca
     2341 ttccaagaaa aacatccaga tcattatttt gacgtgggga tcgctgaaca gcactctatc
     2401 actttcgctt ctgctttggc ttctcaggga gtacgtccag tcgtcttcca atcaagtact
     2461 ttcttgcaac gggcatatga tcaattatca catgatttag cgatcaacga gaatccagtg
     2521 attattttag tcggtggcgg aacgatctca gctagtgacc gaacacacca aggaacattt
     2581 gacattcctt acttatcaag tatcccgaac atcaaatatc ttgctccagc aacaaaagag
     2641 gatatgatct ctatgctaga ttgggcgttc gaacaaacag cgcagcctgt agcaatccga
     2701 attcctacac atggtgtaga acatggtaca gctatcgcaa atgattatag tattccagcc
     2761 tatcaaacag taaataatgg aggaaaagta gcaattttag ctttaggcgg ttttcttgaa
     2821 ttaggaaaag aaacagtaaa agcttttaga aaaaaaggaa tcgaagcaac gttgatcaat
     2881 ccaggaacga tcacagaaaa tgacgtagaa acattagata aattaaaaga aacacacgaa
     2941 ttagtcatca cattagaaga tggtagtttg tcaggaggat ttggcgaaaa tatcagtcgt
     3001 tattatggtg attcttcaat gaacgtatta aattttggtg cagagaaaga attcacggat
     3061 agcgttcctg tagggcagct ttatgaacgt tatcatttaa caccaaaaca aatcgttgaa
     3121 gatagtctgc gtgtcttgaa tattcaataa ttcattatgc tcaccatcca gataattgtt
     3181 tatactagta tctggatggt tttttcatta taaaaagcaa aggggtggat gttatgtatc
     3241 agcaagtaga agaatatgtc aaaaagttcc aagaaaaata tcctagttat caatcagaaa
     3301 aattaggttt cttttcattt ggggatacag aaaaaatggc agacgagttg agcgaactag
     3361 ttgtaaaaaa gataaagact ggtacatcaa gtggttacga tctgtataaa caagagaatg
     3421 agcctttacc aaaagttggt caattagacg tggtcctgaa tggaaaaaat gaaccagttt
     3481 gtatcatcaa aaatataaat gtgaccatct tgccttttag tgaagtcacg aaagagcatg
     3541 cttggaaaga aggagaaggg gatcggacat tagcttattg gcggaaagtc catgctgagt
     3601 tttttaaaca tgcttataaa gaagcgttga acatagaatt tacagaagag aagctggtcg
     3661 tatacgaaga atttgaagta attttctttt agtaagaaag gtctgctgaa agaaatagct
     3721 cattattgtc tcctgaatag aaaagggtta catttttata tgaagctgta tttttatata
     3781 acattactgg aggagatatt gtggatacac tggaacaaat caaaggcgga gtcgtcgttt
     3841 cttgtcaagc tttggaaaac gagccgctac acagttcttt tattatgggg agaatggctt
     3901 tggctgcaaa agaaggcgga gcagttggta ttcgagcaaa tacgacagaa gatatcaatg
     3961 aaatcaagcg aatcgttgat ttaccggtaa tagggattat caaaagagat tacgatgggt
     4021 cagaagtgtt tattactgcg acagaaaaag aagtggaaga acttttggag acgcaagcag
     4081 aaatcattgc actggatgca acttctcgta aacgtccgac agaaaaaaca actgctgaac
     4141 ttgtccaaat gattcacgaa cacggtcggt tagctatggc agatatttcg acttttgagg
     4201 aagcgcttca agcgcaagaa gatggttttg atcttttgtc tacgacttta gcaggctata
     4261 cagaatattc acgaaagacg cagacacctg attttgattt attgaatcaa atcatagaac
     4321 aagtcgatat cccagtaatt atggaaggac atactgactc tcctgaacaa gtagagaaag
     4381 catatgaatt aggtgctttt tcagtcgtag ttggatctat tatcacccgt ccgcaattga
     4441 tcactaaaag atatgtcgaa gccgcaagta aagcaaataa ctaaaaaaca cgcacggcac
     4501 agattcttga caaaaaagaa aaattgatag atagtttaaa gcagattcta agaagatgaa
     4561 aaaagcatta cttttcattt tgtcttcaag atctgctttt taacgaattt tatagaaaga
     4621 aggcgaaacc atgtttgaac ggagtccttc agaattgaca gaacgtgaaa attacaagtt
     4681 gctgattggt tcagtcatcc ctagaccagt agcagtagta acgactaaat cagcaaaaga
     4741 tatagtgaat atcgcaccct tcagttactt caatatcgtc agttcgaatc gacccatctt
     4801 atccttggcg atccagagaa aacaagatgc agtaaaagac acagcaaaga atattcttga
     4861 gacaagagaa gctgttatcc atatccttga tgaggacaac gtagaagaag taaataaaac
     4921 agcagccaat ctgcctaatg aggaaagcga actgtacctg actgacttga ctttaactga
     4981 ttcagtaatc atttcagtac cagcattgac agaagcaaaa gttcgttttg aaacatcctt
     5041 atatgaacat gtcgagatac ggaataaaaa taaagtaaca gctgatttat ttttgctaga
     5101 agtacagaaa tatcatttaa atgaaacagt ctatgatgat aagacaggaa ggatcgatcc
     5161 aagaaaattg aatgctgtaa gccgtttggc aggaaatgat tatgcagcaa taggagaaat
     5221 tttcacgatt gcacgtcccg aataaaaagg agcactagtt atgttaaaaa taagctataa
     5281 accatcgaca gattcaaaag aaatgaaaaa agaatatgaa acagtgaatg attttctgca
     5341 agggcagtac ttagaagttc cgccattgca agaccatttt gtcgttacta ctgttacttt
     5401 agatggtaag gaaatagaaa tgccagatca aacgattagc ggattgttta attac
//
LOCUS       DASNNU010000128         5215 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.131, whole genome shotgun sequence.
ACCESSION   DASNNU010000128 DASNNU010000000
VERSION     DASNNU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.131"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(559..1599)
                     /locus_tag="U8116_002560"
     CDS             complement(559..1599)
                     /locus_tag="U8116_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN8435491.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1948..2274)
                     /locus_tag="U8116_002561"
     CDS             complement(1948..2274)
                     /locus_tag="U8116_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435492.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2261..3064)
                     /locus_tag="U8116_002562"
     CDS             complement(2261..3064)
                     /locus_tag="U8116_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN8435493.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3417..3707)
                     /locus_tag="U8116_002563"
     CDS             complement(3417..3707)
                     /locus_tag="U8116_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEN8435494.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3843..4022)
                     /locus_tag="U8116_002564"
     CDS             complement(3843..4022)
                     /locus_tag="U8116_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435495.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4019..4369)
                     /locus_tag="U8116_002565"
     CDS             complement(4019..4369)
                     /locus_tag="U8116_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435496.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4362..>5215)
                     /locus_tag="U8116_002566"
     CDS             complement(4362..>5215)
                     /locus_tag="U8116_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HEN8435497.1"
                     /translation="IATVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIP
                     EMTDFWGIGSRMKKRLNQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDI
                     AIPPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIG
                     TSILETRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSL
                     QLDLFSDPQKQIDEENLDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGG
                     NGGIKND"
BASE COUNT         1551 a          942 c          753 g         1969 t
ORIGIN      
        1 cgttgcgggt aataccgtaa cactgaaggg cacgcattaa ggcgtcatct taatgctttc
       61 ctgtaggaca ctgacggaga ttaccagtct cccgagcctt acaggggcct ttcttttttt
      121 tgtattttca attatcacaa tttatcgtcc aaaagtgcag aacgaaaatc aattcatcac
      181 aagcatatca gacaattaaa tttaaaacaa ctaaaaatat acaaaaaagt gtaattgaat
      241 attagcagca ctgcctgaaa agaagtaata tcaggctttt cagcttgggg aaataagtga
      301 attacctctc ttcatgaatt attataactc aaaaaagcac atatgcaaac actagttccc
      361 tatttttttg aatgaaaaat aactaaaata tctaacgatt atgactttaa aacctacgtt
      421 tgtgattgtt ttcataaact tttaatctaa aaactccgga ataatagcta tttatttgta
      481 acatttgtaa taaaattaaa atcataaaaa agttctaatt taaaattgat catttttata
      541 gtgatcgctt tttatcaact actctagcca attttccgta ctaactgttg gtaattcgtt
      601 aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcga caaaccaatt
      661 ttttacataa acgaaaatta gattctgcaa attgtttact ttttcagttt tttgtttttg
      721 ataagctttc agcaaagtca tatataatcc tttgtcagca tcaattccat aagattccag
      781 ttcttcataa acaacaactg tttgctctaa ttcttcagct tttttctttg cattatgaat
      841 agtttggacg gttttttgag cgtcttcgta agtactagaa aatgttttta tcaagtttaa
      901 tacattttct ggtacaaatg tatttatact gtcctctttc atgatgtcga cgaagttatc
      961 tagtaaaact tgattttgta actgatcagt ttcagtgtct attatgtgtc tattagtgtc
     1021 tatagaatta gaatagaata gattgtctgc gattttcgca attacggttg agtccttgat
     1081 atgactggat tttgccgtat ttgcgatttt cgcaattacg gtggtatcat tgatttctcg
     1141 cggttttgcc gtatttgcga ttttcgcaat tacggtggta tcagtattgt cttcctcttc
     1201 catgatttta aagacgtctt gattcgttac ttctggcttg cctaagtaca ataaattagg
     1261 catggattca atcttaccgt cacgtttatt tactcttcct ctcttctgtt tcagaagacc
     1321 tgctttttct aattcctttt tgattttaga tactttacct tcgcggcatt gtaacaattt
     1381 cattaattct tcaactgtga atatgaaaaa tatattatca tcagaatcta cccagttatt
     1441 tttgactgaa taatcaaatc gatctttaag tagcatataa gcaatttttg catcatttga
     1501 tagatccatg tatttttcgt tagtaaaaaa taccttaggc atttgataat atttttcacg
     1561 ataaatttct tttgatttgt aataattaaa atcattcata gccaatcctc tttctgtagg
     1621 atagcgtttt gttcaaaact atgttacaat gaattaaaca aatatatcta tccactatat
     1681 atttttctac tgctgataag tcgccaaact ttgttaaagc agtagtcttt ttttatcttt
     1741 ttttagttta ccttaaaaaa taaattaaag caaaccgctc tatttcaagg tttaagctca
     1801 tagtaactat atcacgatat tacaataata ttatatcacg atatcgtaat attacaatat
     1861 gaagataagc gacttatcag ctttgatact actgattttt ataaaaaaaa tatcacgata
     1921 ttatgatatc gtgatattga atgtaaatta aattttattt tgtttctttg ctagtttata
     1981 tccttcaatc aagtattctt caggtatgga ctttaatagc gcatcttcta taacgtctgg
     2041 tttacttttg tctgattcag caatagtcgt ccaatatttg agtgctgaca ttagaattgg
     2101 atttatacga atgctatctg ctgttcttac actacttcct tgatctttta tgactttact
     2161 tatatttttc ttggttaatt cgatttcttc tacttctttt gatttaaaat tctttttatt
     2221 tttatttcct agtaatctac ttactgcagc ttgactgcga ttatcttctg ccatcaaaat
     2281 caccaacttc ttttaaatat tcttctgtaa atgctttata gtcatcttca gctgttgagg
     2341 gtttattaaa attgattaga tctgatccag cagcttgcgc ttcgtcaacg gttattgtcc
     2401 tacgaatata agttttgaaa atttttgcac caatgactct agaaatttcg gttgctgcag
     2461 acgtcatttc tttagaaatg ttataacgct tattatactt agtaaataga actcctcgta
     2521 cttcaagctt gtcgttcgta tattctttta ccgtttcaat agtttcactt aactttatta
     2581 gtccttttaa tgagaaatac gcagcttcaa ctggaatgat aatttcatca gaaacagtaa
     2641 atgcgttgac tgtaagcatt cctaaacttg gtggacaatc aattataata taatcatagt
     2701 tgtccttgat tggttgaagt actcttttaa gcctgtgttc acttcctact tgagtaaatt
     2761 ccctctctgc agaagaaagg gctatgtcgg ctggcaaaac atcaattcct ttatagtcca
     2821 gaatagcatc tgtaatgtca gcctcccctt ttaatacttc ataaattgta ggttggtttg
     2881 ttccatctat atcgaaacct acattatctg tagcatttcc ctgaggatca atgtccacta
     2941 aaagtacatt tttaccgtaa acaaatttca aacatgctgc taaattaacg ctagtcgtag
     3001 ttttagaaac tccacctttt tgattggcta ctgacaatat tcttgccatt aaaatatccc
     3061 ccattcatct tatattataa tataattata tcataatatt gtgatattgc aatatcgtat
     3121 atgcattata ttgtaatatt gtgtgattat tttgttttaa agatatatat cggttcggat
     3181 ataaagatta ttcatatgtt attcgaaatt aaaataatca ctttttatta ctttaaaatt
     3241 ttttattaat gataagaaat atgttttgcg agcagcaact agaataattt aaacattact
     3301 aaactgtcta tattgttact aataattccc ttcttatagt gcattttctt agaaaaaaac
     3361 aatattacga tatcatgata ttaagatatc gtaatattat acaattgtga ttgatattat
     3421 atatgttttt ttattttttt atcatgttta atcttgaagg aatcatattc tttttctaaa
     3481 gacaggagct tttcataaac atcagacatg tttaaactag aggccaggct taaatcataa
     3541 acgaatgaag caggtaatga ttttattcta cgttgtcggg ttatccagct tgcaaccgtt
     3601 ccttgtgggt aggcatataa ctcacaaaat tgttctacag tgattcctaa attcttgata
     3661 atgtaaacat tgatcgggtg cgagtaaaca aatgtattga tagacatggt gcgtaagcct
     3721 actttctttt attattgcgt ttgcaataat agtttaacac acgcattatt ctagtatcta
     3781 ctcctatttg taaaaacaaa aaaggattca tagagataac tctatagaat ccgtaaaata
     3841 ccttatatat ataacgggaa gatataaaac ctaaaattat gataacctgc tgcagcttct
     3901 acatagaaag agaagcgctc aacaccaacc tcatttatca aacgttggcc ataaccccaa
     3961 atctcttctg atagctgatc aaaggttata tactttagct catattgctg gataatgttc
     4021 attctaatcg actccattta ttatcctggt aaaattcgat atgacgtatt tcgtcgtagt
     4081 gaattttgtc ttttccaacg tagatcccca gctcatcact acctaagatc ttgccaatga
     4141 tatcttctgg atagtatcct tcagcgtttc gttcttcttt ttgaatagat actgatctat
     4201 tttttatgac tgcagcgttc aatacttccg caatctcttc agtcgtcatt tgttcttttg
     4261 ctaaattttg actggttcgt tgttcattgt cggcatcaat ttcttcagta tgatcagata
     4321 aataaaaccc tgcccacttt aaacgtccac gatcattgta atcaatcatt tttaattccc
     4381 ccatttccgc ccgcatgacc accaacaagt gtgcttctag taatgcttct agcgccttct
     4441 aacattgaag aagcatgtac aatcgacgta aaaccatatt tttgccgaat cttgtcaata
     4501 attttatcta aattttcttc gtcaatttgc ttttgtggat cactaaataa atccagctga
     4561 agcgaatcgg tgtacacaag cttgctatag gttattccaa catgccttac ttcttgtcct
     4621 tcataatact tatcaaacaa gaaaagacaa taattctgca attcctttgt attatctgtg
     4681 atagggatct tcatctggtg tgaaaaccct ggtctcgttt ctaagatcga cgtgccaatg
     4741 tgtaaatgga cacaacctgt tttacagtta tgctgcctaa tgcgtatcgc tacttgttcc
     4801 gccatttctt ttaccaccag ctcgatttca tttcttctag tataatctcg tggtaacact
     4861 tgtgaatttc catatgattt ttcctttgtc ggttctggtg ggattgctat atctgttcga
     4921 tcgatcccat tagcatggaa gtacagctgt aagccaatca cacctagtct attcttaatc
     4981 gtataaggat tccagttagc aagatctcta atgctcataa tccccatctg attgagtctt
     5041 tttttcattc gagagccaat tccccaaaag tccgtcattt caggaatatt ccatactttt
     5101 tcaggaacgt tttcgtacgt ccattccgca acaaatcctt cattatgctt tgcttcgttg
     5161 tctaaggcta gtttagccaa aaggggatta tctccgacac ctactgtagc aatca
//
LOCUS       DASNNU010000129         5151 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.132, whole genome shotgun sequence.
ACCESSION   DASNNU010000129 DASNNU010000000
VERSION     DASNNU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.132"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(604..894)
                     /locus_tag="U8116_002567"
     CDS             complement(604..894)
                     /locus_tag="U8116_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435498.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1296..1532
                     /locus_tag="U8116_002568"
     CDS             1296..1532
                     /locus_tag="U8116_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435499.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1539..3491
                     /locus_tag="U8116_002569"
     CDS             1539..3491
                     /locus_tag="U8116_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN8435500.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3563..4060)
                     /gene="dfrG"
                     /locus_tag="U8116_002570"
     CDS             complement(3563..4060)
                     /gene="dfrG"
                     /locus_tag="U8116_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN8435501.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4387..5079)
                     /locus_tag="U8116_002571"
     CDS             complement(4387..5079)
                     /locus_tag="U8116_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN8435502.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1662 a          900 c          861 g         1728 t
ORIGIN      
        1 gatgcaaaag aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt
       61 tcttagttaa ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga
      121 catacaatca tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa
      181 tccaaatgtt aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt
      241 caatttactt ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg
      301 aacgtttaaa atcctacaat tccactacct gagactagta atgactagtc tctttttatg
      361 gcctctgatc cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt
      421 taagcaatat ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata
      481 catgtagtta tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc
      541 atcgtctgca tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt
      601 tttctagttt attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata
      661 ttctaccaca attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct
      721 attattataa taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt
      781 caaactatgg gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct
      841 ggcactaact tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt
      901 cacctcaata ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc
      961 tttttgactt tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga
     1021 aggacatgta ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta
     1081 caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt
     1141 ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta
     1201 tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt
     1261 tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata
     1321 agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat
     1381 acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt
     1441 tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg
     1501 aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag
     1561 ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag
     1621 atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa
     1681 tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt
     1741 actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa
     1801 aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta
     1861 tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt
     1921 tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag
     1981 atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat
     2041 tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc
     2101 aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt
     2161 atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt
     2221 cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg
     2281 agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa
     2341 caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg
     2401 tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa
     2461 cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct
     2521 atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc
     2581 atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac
     2641 catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta
     2701 ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact
     2761 attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact
     2821 tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac
     2881 atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg
     2941 gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc
     3001 tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag
     3061 aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc
     3121 cattagccac taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc
     3181 tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct
     3241 ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg
     3301 gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta
     3361 caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt
     3421 tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt
     3481 atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt
     3541 tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat
     3601 agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt
     3661 cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga
     3721 tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa
     3781 aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac
     3841 aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg
     3901 ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat
     3961 ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca
     4021 ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa
     4081 ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt
     4141 tagttgaata agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat
     4201 tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat
     4261 gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa
     4321 aactgaacta cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa
     4381 gaaagattat ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc
     4441 cacttcatct tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc
     4501 agttgcttcg tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc
     4561 ttgtgtcagt gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag
     4621 ttttgcttgt gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc
     4681 atttttttgg ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg
     4741 tgcatccttg ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt
     4801 ttggttttct aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg
     4861 caatgtctgt tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc
     4921 tgtcaatgta tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc
     4981 accccacaaa ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa
     5041 tgcaacgaca atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa
     5101 tacttttaca tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt t
//
LOCUS       DASNNU010000130         5124 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.133, whole genome shotgun sequence.
ACCESSION   DASNNU010000130 DASNNU010000000
VERSION     DASNNU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.133"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            44..1185
                     /locus_tag="U8116_002572"
     CDS             join(44..272,272..1185)
                     /locus_tag="U8116_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN8435503.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1420..1872
                     /locus_tag="U8116_002573"
     CDS             1420..1872
                     /locus_tag="U8116_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435504.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1886..2575
                     /locus_tag="U8116_002574"
     CDS             1886..2575
                     /locus_tag="U8116_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435505.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2700..5051
                     /locus_tag="U8116_002575"
     CDS             2700..5051
                     /locus_tag="U8116_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEN8435506.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1753 a          776 c         1047 g         1548 t
ORIGIN      
        1 atttaaaact ttgcaacaga accaagaaaa ggacgtgtcc acgatggcaa aagccaagaa
       61 aaaatttgat gaagacttta agaagatgat cttagattta aaccagtctg gccaatcggt
      121 cgaagaactg gcagcgcaat atggtatcgc tacacaaacg atttatcgtt ggaagaagct
      181 tcatacaaag aatgtagcaa ccggcatgac tgaggcagaa atcctagcga tgaaaaagga
      241 aatggcccgc atgcaggagg aaaacactat cctaaaaaag gctttaacca tattcgctca
      301 aaagtaaaaa tgagtgatgt cttcgaattt attcaatcaa atgttcatga tcatgacgta
      361 aaacaaatgt gtaccgtttt ggaagcaccg aaatccagct actatgactg gaagaaaaag
      421 aaaccttcca agcgccaatc agaaaacaaa cgcttaaacc gcttgatttc aggaatctac
      481 tttgaaaaca agggtatcta tggtgcgcct aaaattcata agattcttgt tggccgtggc
      541 gagaccttgt ccttaaagaa agtacaaata ctgatgagaa aattaggctt gcgttcagtc
      601 accctcaaga agtacaagcc tggcaaacaa gcgaaagtta aaagtgaagg acgtaaaaac
      661 ctgttaaacc aagattttac cactacgtcg atcaatcaaa aatgggtgac agatattacc
      721 tatattcata ccctaaaaga tggctggacc tacctctcca ctattcaaga cctccacaca
      781 aagaaagtca tcggttggaa gtttggcaag cagatgacga aagaattggt tatcgaaacc
      841 cttgacaatg cgctcttaaa ccaaacaccc tccgagaact tgatcatcca ctcagatttg
      901 ggttctcagt acactagcga agcctatgag gcaaagctga atgaattgaa cattcggcac
      961 tccttcagtc gtaagggctg tccctacgat aatgccggta tcgaatcgtt tcacgcctca
     1021 ctcaaaaaag aagaagtcta tccatcaaaa gcgtatgaaa actttgaagc tgcttattta
     1081 gcgttatttc aatacattga aggattttac aatcgcaagc gaatccatag tagtatcaac
     1141 tatttaacac caaatcagat ggaggagttg gcattgcaag cttagttcct agctaccact
     1201 tacatttttt tggcgagatt tccacagggt ttcaacttca tcgcttcgat cagcggtcaa
     1261 acggttttgg tttgaccgct ggtcgaaacg atgagatact cacccgctgg aatctcgaaa
     1321 aaattctcac aaatcgtgtc cgaggtattg actcaaatcc accaattatc ttatcgtaga
     1381 tatatattat aaatttaata agaaagcgag gtgagaatcg tgaaaaaaac gatactaagt
     1441 attttttcag tatttacaat attaattggg ggctattttc ttttgaattt agattctgtt
     1501 aattcaaacg ctcaaataaa agatcaaaga gtagctgaac ataataataa aaaaatgagt
     1561 ttgaccaaaa atatctattc tatgtcagat gaagaattgt ataaagaatt tggtgattta
     1621 tccggggaac agttagaaga aagtattcca ggaattagtg actcagataa aattgttgtc
     1681 ttgtctgctg gtgggcaatt aatgggagat ggcaaatccg aattagttag ttataaacaa
     1741 aatcacagac agtttattga tgttaatact gattccaacg ctatttcgat taaggatcta
     1801 aaagagcttt taaatagtaa aagaaatgaa ttaaagcaga taaaagaaac taattcagtg
     1861 gaattagagt aaaggaagat tcattatgaa aaagattttt ggtgtttctg ttgggctact
     1921 tatgatgtta acatttggaa atgcagtata tgctgctgag aaagaagtta tttcagatcc
     1981 agagcagtac gcctcacgat actttgctga ttttgaagaa gaagataaag tacttgcatt
     2041 gccagaaggt ggttttttaa aaggggaagc taaaattgta tctatggata atccagatat
     2101 aattcaaggt tcatataatt cagaaactga tcctgcttca gtttcagtga cagaagcaaa
     2161 aaaaattgtt gctgaaaaat tcaaaaatga tattgaaact actccaatta tgcctcgagg
     2221 agcatttcca cccgatgttg gtactagcgg acaccttctt gtactagcag gaggaggaag
     2281 ctataaatca cagccattta gtggtgcggg atggcgtttt ggaggtatgg gtgtaaaacc
     2341 agctgatggt acaggcaact ttttacgttg gaaatcctac attgacgatg gaagagtagg
     2401 aaatgtaaat caagccaacg caactaaatc tggttctatt caaggaatgg cattgtatgc
     2461 tcgtgaagga ttcaaatact ttgcaccaaa cgggcctggt tggacaatga atataggtat
     2521 ttattacact tataatcctg ttgcaggtac gtattatgtt gttcaaaata tttaataaaa
     2581 cagcacaaag agatttcttt gtgctgtttt attattttta gttgttgaca aacactaaaa
     2641 tataaaatat aatcaacaca taaacgaacg tatgttcggc aagaaagcag gggattttta
     2701 tggatgagat ggttttaagt actcagaaat ggctaaacaa gaaatacagt aatgttacag
     2761 ggtttgataa ggttccagaa aatgggcgaa cagggtggcc tacaatctat ggacttattg
     2821 aaggactaca agtagaactt ggtattacta atctagtagc taattttgga cctaccactg
     2881 aaaaaatgta cgataatcag gttactccta aatggggcaa aaatctacct aaaaatattg
     2941 tatttcttat tcaaggtgcc ttttggtgta aggggattaa tccaggtggc ttcgatgggg
     3001 tatatactcc atatttagat actgctgtaa aagagttgca gactgatgca gggtttaatg
     3061 gaagcgctgt aactggagtt ttggatgcga aatgggccaa agcgttattt gatatgagtg
     3121 cttttgttct tgttcccggc ggagatagta aaattagaaa aatgcagcaa tggttaaatg
     3181 taaattattt agaatacacg ggaataatgc cttgtgatgg tatttatcaa agaaattcaa
     3241 atcaagcatt aatttttgca ttacaagctg aactaggata ttctcccagt gaagcaacag
     3301 ggtcttatgg taatgggaca actagtaaaa cttatcctgt gtctgaaggt aactcgggaa
     3361 actatgtaag aataattcaa tatggattat atgttaatgg ttattttgaa gacggtacat
     3421 tcgatggtat tttcactaaa tatatgggtt tagaagtact ggcattccga aaatttatgg
     3481 tattgcctga atatactgaa atagcagatg ttgttgtaat aaaaggacta ttgtctagcg
     3541 ctggagatat tagaagatct gcagatggcg cagatacttc tacgcaatta actagatctc
     3601 aaatccaaac attggttgat aatgatataa aaatagttgg tagatatctt actggtactg
     3661 ttggaattgg aaaagatgaa cgaaataagt atctaactac agaggaattg aataatattt
     3721 tttctaaaaa tttatctgtg tttcctatct atcaagatgg aggtgcagca ttagcttatt
     3781 ttacttatga tcgtggctta tcagatggta agaaggctat agatgcagca aaaaatctaa
     3841 atataccatt aacaacagtt atttattttg ctgttgatct agacatgtta ggggaagaaa
     3901 ttcttgcata tgctggagaa tattttaagg gggtatctgc tgtaatgtca tatagcgggt
     3961 atcaaacggg tgtttacgga acaagaaatg tttgttctca aataatcaat aacgggtatg
     4021 cttcgtttgc atttgtttca gatatgtcga ctggttatag tgggaattta ggttttccta
     4081 tgcctagaca atggtctttt gatcaatttg ttgaatttac cataggaagt ggcaacggag
     4141 ctgttggtat cgatttaatc gctacatcgg gtagagatag cggatttaat gagttatcaa
     4201 atgatactaa taatgataat tacattgcca aatacaatca gaaggttata aatcagatgg
     4261 tacgtgcgta tcaatatcta tcacaagctg gtcttgataa tccttggaac cctcatttga
     4321 cattttatag atatgtaaat tattctggtt tgtcatggga catcatttct tctcctgtaa
     4381 cagagcatga tagaaaaatt tatgatgaat ataggaataa attaactaat agcgagggcc
     4441 tgtataatta ttttatagat cctaatactg gttcaataat aggtttgcct cacttaattg
     4501 tgacgttaca atcacagatg tttctagccg acacaatcaa tgatagtgtt tctgactttg
     4561 caggttggtt aggagattta atgacttgct ggggtgaagt taagaaatta ggaatacaaa
     4621 tggagcaagg tgtatttgca ttagtaggta cggctgcagg tggaacattt agtctagaag
     4681 atttatacca agatgttgac gcggtaaatg tttatacctt agcaaaaaga aattctgatt
     4741 caacagcttt atcggcttta aaaacgtact attgtgataa gggtgctatt gatagattta
     4801 atcaatttgt taagaacaga tttggttctt cagataaaat cgcttcagta acaaatagtt
     4861 tattagaaaa tgacgaaaca gttgatatct taggtgtagg gataatttca tttttcattg
     4921 ataaaatagt taataattca cttgggacag attttactta tcttgatttt attggagatg
     4981 aggggaaagt tgcaaatgga ttttctgaaa aaattattca atttcaagaa aatagaagta
     5041 aacttaatta agaatcgttt actatttaaa cagtgtcctt ttccttttta aatatatacg
     5101 ataaaataag tatatgaatt gtaa
//
LOCUS       DASNNU010000131         4972 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.134, whole genome shotgun sequence.
ACCESSION   DASNNU010000131 DASNNU010000000
VERSION     DASNNU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.134"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="U8116_002576"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="U8116_002576"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEN8435507.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="U8116_002577"
     CDS             complement(1297..2133)
                     /locus_tag="U8116_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8435508.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="U8116_002578"
     CDS             complement(2317..3534)
                     /locus_tag="U8116_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN8435509.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3862)
                     /locus_tag="U8116_002579"
     CDS             complement(3683..3862)
                     /locus_tag="U8116_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435510.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3917..4000
                     /locus_tag="U8116_002580"
     tRNA            3917..4000
                     /locus_tag="U8116_002580"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4818)
                     /locus_tag="U8116_002581"
     CDS             complement(4240..4818)
                     /locus_tag="U8116_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435511.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1525 a         1027 c          798 g         1622 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gc
//
LOCUS       DASNNU010000132         4933 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.135, whole genome shotgun sequence.
ACCESSION   DASNNU010000132 DASNNU010000000
VERSION     DASNNU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.135"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            85..972
                     /locus_tag="U8116_002582"
     CDS             85..972
                     /locus_tag="U8116_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN8435512.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="U8116_002583"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="U8116_002583"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEN8435513.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="U8116_002584"
     CDS             2274..2720
                     /locus_tag="U8116_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN8435514.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="U8116_002585"
     CDS             2993..4345
                     /locus_tag="U8116_002585"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEN8435515.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="U8116_002586"
     CDS             4516..4803
                     /locus_tag="U8116_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435516.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DASNNU010000133         4927 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.136, whole genome shotgun sequence.
ACCESSION   DASNNU010000133 DASNNU010000000
VERSION     DASNNU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.136"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..2083
                     /gene="pglX"
                     /locus_tag="U8116_002587"
     CDS             <1..2083
                     /gene="pglX"
                     /locus_tag="U8116_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HEN8435517.1"
                     /translation="NKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMT
                     PFVWMFISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIK
                     LSDFKGGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQ
                     GKLTNDVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGG
                     RFRKWYGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVS
                     FRLQNQAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLP
                     FLKVTSERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCL
                     SRQLVNQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISY
                     SIGCIFGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRF
                     IDFVEITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQ
                     LDSGKQNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKER
                     NAMTKEIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQ
                     IFAKGVKEPKK"
     gene            2199..4721
                     /gene="pglZ"
                     /locus_tag="U8116_002588"
     CDS             2199..4721
                     /gene="pglZ"
                     /locus_tag="U8116_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HEN8435518.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
BASE COUNT         1809 a          670 c          921 g         1527 t
ORIGIN      
        1 taataaaatg gataagaaat taaaaaaata tgtaaatgat aactattctg atgtcaaaaa
       61 agatctattt tctgtgttta ttaaattgaa tagccaaatg ctgattgata atggatatgc
      121 ttcctttatg acgccatttg tttggatgtt catttctagc tatgaaaagt tacgctcatt
      181 tttaattaat gaaagaaaca tttctagttt gattcaaatg gagtattcag cttttgaaga
      241 agcaaccgtt ccaatttgta cgtttacgat aaaaaattca aatgaacaag atggaagcta
      301 tataaaatta tcagatttta aaggtggaat ggaagttcaa aaaattaaaa cgttagaagc
      361 aattcatcag catggtgaat gcgattattt ttatagtact aatcaagaca acttcaaaaa
      421 aatcccagga atgccgattg cttactgggc aagcgataac gtaatcactg attttgctca
      481 aggaaaatta actaacgatg ttgcagatgc aaaacaaggg ttggcaaccg gaaataacaa
      541 taaatttttg cggtattggt ttgaagtaga atttaacaaa ataaagtttg atgctaaaac
      601 attagaggac gctaaaaagt ctagaaaaaa atggtttcct acaactaaag gtggtcgttt
      661 tagaaaatgg tatggaaaca atgaatacgt ggttaactgg gagaatgatg gagatgaaat
      721 aaaaaattat gttaatgaaa atgggaagtt aaaatctaga ccacaaaatc ttaattacta
      781 ttttaaaaag ggaattactt ggtcgacact ttcatctgga caagtatctt ttagattgca
      841 gaatcaggca atttttgaaa ctaaaggatc tgttctgttc tcaaaggata taactgactt
      901 tgaagaaata tttggctatg taaactctaa attagttaat cactttttaa cttttatttc
      961 tcccaccttg gattaccatg aaggaccaat gggaaaacta ccttttctta aagttacatc
     1021 tgagagaaaa ttaaaaaaaa taagagaaaa agtggatgaa aatatcgata tagctagaaa
     1081 agactataat gctcttgaaa cgtcatacga gtttagaaga aacattttgt taagttatat
     1141 gtttaaaaaa gatctctcag aggcatttaa ctgtctaagt aggcagttgg ttaatcaatt
     1201 taatactcta aaatcgaatg aagaggaact gaacgacata ttcattaatc attattctct
     1261 tgaaaaagaa ttaacaaaag aggttcaaga tgaatatatc tctgcaaggt tacctgataa
     1321 ggcgagggaa attaaggatt ttatttctta ttcaataggt tgtatcttcg gtcgttactc
     1381 cctagacgaa gatggtctca tcttcgctgg cggcgaatgg gatccttccc gctatcaaaa
     1441 atttaaacct gatactgata atgttttact catcaccgat gatgagtatt tcaaggatag
     1501 ccaaattgac attgtcaatc gtttcattga tttcgttgag attacttttg gtaaagaaac
     1561 ccttgaagaa aatctccagt ttatcgctga cactttaggt ggcaaaggga caagtcgaga
     1621 aattattcgt cgttacttcg tacaagattt ttacaaagac catttaaaaa catactccaa
     1681 tcgaccaatt tattggcaat tggactcagg aaaacaaaat ggctttaaag cgttaatgta
     1741 cttgcatcgc tatacaccag atcaattagg aaaagtacgt actgaatacc tacacgaact
     1801 acaaaaagcg tatgacaatc gaatcgaatt gcgtcagcaa caaatttcaa caagtgataa
     1861 cccaaaagaa agaaatgcaa tgacaaaaga aattgtaaag attcagaagc aattaaaaga
     1921 agttcgtgag tatgacgaga agttgggtca cttagcatta aaacgaattg aattagactt
     1981 agatgatggt gtaattgtca attatgataa gttgcagcgt gatcctaaaa ccggcgagaa
     2041 attccaaatt tttgcaaaag gggtcaaaga accaaaaaaa tagttatcta agaaagttgt
     2101 tttctaacaa ctttcttttt tattcttaaa ttgcgtgcga aattatccaa attatgtcaa
     2161 tataaggggt atagaaagag aacgattagg tgagattgat gacagatata aatacgcaac
     2221 aaattgaaga aaaattaaat caacgttttc ctaattatgg atgtaggaaa atcatttttt
     2281 ggtttgattc aaatcaggat tttcttgaag aaattgactc attggtcatt caaaatgcaa
     2341 aaatctatcg attagaaaag gacgcacaat ttaaaactaa gcgtttattg gaatatgaag
     2401 atcccgaaac taactattta atctatgcgc catttcctcg ttgggatgat aaagatgaag
     2461 ataatcattt gttgagtgta ttgaattatt cagaggaatt tagtgcagac cgcttagcga
     2521 ttatcatgaa tgaattggct attccactta gatttcatca gacagttaaa cagtatcgta
     2581 atttctttaa tgcaaaggat cgcatgagaa gttttggtga atttagtacg catatgacaa
     2641 ttaataaatc agaaacgatt gaattaatga taatggctgt tttgttgaaa tcgaaaagta
     2701 ttcgattaaa tgatttactg cgtgtagttc taaaaagctt tacagaaagt agggagaatc
     2761 ctttaaggga acttgataag tatcagttaa cagctaaatt ttgggaattt attcaaatga
     2821 aatttggctt tacggatagc cagccaacat tggaaaagct gacgatgtgt ctttatttga
     2881 actattttta ttatcaaatt gatcaagaga tgccgaagaa gttcaaagga tttgaaatac
     2941 tacatttgaa agcaaacatt attactttca tggatcagtt tatgaatgat acacgttaca
     3001 tggaaagctt tgaacagcta tctaaaaaag tatatcaggc tattgatggt gatggattat
     3061 tagacagtat agaaattgat gatctaattg aagcagacgt attttctgag attcatgcaa
     3121 aaatattaat gttttatgca gaacgattaa aggcttctga cactacaacg aaaattgcag
     3181 gacaaacaat ttttgatagt ttaaagcagc gcgaaaaaat gcatttcgca tcggattata
     3241 tttatgagta tcaattattg aaacatgctt attatcttgt ggataaaaac agattgtgct
     3301 tgccgatgaa catggataat ttgctaactc aatatgagaa ggaaatgtat tgggttgata
     3361 cgcattatcg gaaatttatt tggaatagtg atcatataga agaaaagcaa aaatatggcc
     3421 aattgtatcg attagttgaa gcaaattatt cgaagtttct tgatcaatca ggacaaattt
     3481 ggaatcaaac ttttgaattt aagaatcgtc catccattcg aaaattttat aatcaagctg
     3541 taatgaaacg ctctgttaaa actgtagtta ttatttcaga tgcttttcga tatgagttag
     3601 ggatgaattt agaacgtaag ttagttagag aaaagaagta ttcaacaaac atggctagct
     3661 ttctttctgt attaccttca gttactgaat tcggtaaagc cgccttatta ccaaacaatc
     3721 aactaactta caatgaagga acagaagtac tgattgatgg gaaaaaaacg aatggtttat
     3781 ccaatcgaaa tgcgattctc cagcaacgag atgcaaatgc tgtagcttta aattatgatg
     3841 atgtggttaa gatgaataag gctgaggtac gagaagtatt taatggcaaa tcagtcattt
     3901 atatttatca cgatcaagtg gatagaacag gtgatcactc gaatgaaaat cgagtttttg
     3961 aggcctgtga tttagcgata agcgaaattt cccgacttat taatcgcttg cataatgatg
     4021 gcagtatcta tcgttttata gttacggctg atcatggatt tatttataaa agaagccacg
     4081 ttgaagaagt agataagatt gaaaatccat ctagaaatga aaaagatcgt gtagaacgtc
     4141 gttttattct tagtgaagaa gaatacgaaa taattggtac gaaaaattac tctttgggag
     4201 attctttagg aaatgatgac actagaaaca tttgtgtccc gattacttca agtattttta
     4261 agaaagctgg cgggggacaa agctacgttc atggagggtc atctattcaa gaaatgctag
     4321 tccctgttct agaaattacg gtagccaaag gtagcaatgc aaaggaacca gtagcaattg
     4381 aattaatgac tagcaaccgc agaattacag gattatcaac cacgttagaa ttctatcaaa
     4441 aaaatgcaat tagtgatgtt tatgaccccg catctttctg gctttatttt gaagatgaat
     4501 atggtgaacg tatctcaaat gaagaaatct atgttgcgga ttcaaaaaca gaagatgcaa
     4561 atcagcggtt tactaaattt gtttttgaat ttgtaaatcg tcgatataca gttgaagata
     4621 cttattattt agtgataaaa aatcaaaaaa atgatttgac cgagcgaata aaattcatta
     4681 tagataatcc ttttgcagaa tcatttgatt ttgatatata aaaacgaggt atccacaatt
     4741 ttttgtggat actttttctt ttgtattatc aagcctactg gttaagtatg atatacttta
     4801 attgctatta taatgactga acacatggag gaattctagt ggaacaacag gaaattttaa
     4861 agttatctga attctttccc ggaaagattg tcagaaaaga tttaacaaag aaaattaaag
     4921 aaggtgc
//
LOCUS       DASNNU010000134         4893 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.137, whole genome shotgun sequence.
ACCESSION   DASNNU010000134 DASNNU010000000
VERSION     DASNNU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.137"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(287..649)
                     /locus_tag="U8116_002589"
     CDS             complement(287..649)
                     /locus_tag="U8116_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435519.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="U8116_002590"
     CDS             complement(723..1103)
                     /locus_tag="U8116_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEN8435520.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="U8116_002591"
     CDS             1167..2360
                     /locus_tag="U8116_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN8435521.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="U8116_002592"
     CDS             complement(2437..3021)
                     /locus_tag="U8116_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN8435522.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="U8116_002593"
     CDS             3222..4220
                     /locus_tag="U8116_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN8435523.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="U8116_002594"
     CDS             4332..4841
                     /locus_tag="U8116_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEN8435524.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1553 a          914 c          852 g         1574 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttg
//
LOCUS       DASNNU010000135         4828 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.138, whole genome shotgun sequence.
ACCESSION   DASNNU010000135 DASNNU010000000
VERSION     DASNNU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.138"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(23..397)
                     /locus_tag="U8116_002595"
     CDS             complement(23..397)
                     /locus_tag="U8116_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN8435525.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(420..1820)
                     /locus_tag="U8116_002596"
     CDS             complement(420..1820)
                     /locus_tag="U8116_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEN8435526.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(1824..2234)
                     /locus_tag="U8116_002597"
     CDS             complement(1824..2234)
                     /locus_tag="U8116_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN8435527.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2252..2743)
                     /locus_tag="U8116_002598"
     CDS             complement(2252..2743)
                     /locus_tag="U8116_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN8435528.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(2753..3544)
                     /locus_tag="U8116_002599"
     CDS             complement(2753..3544)
                     /locus_tag="U8116_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN8435529.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3537..4310)
                     /locus_tag="U8116_002600"
     CDS             complement(3537..4310)
                     /locus_tag="U8116_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN8435530.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASIPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4466..>4828)
                     /locus_tag="U8116_002601"
     CDS             complement(4466..>4828)
                     /locus_tag="U8116_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN8435531.1"
                     /translation="LNELVKLFTIEGASTKLRFLDSKKVFNEIEKVLYLYEKYYDVKI
                     PSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIE
                     IPKGEYELIYTIFEQTIF"
BASE COUNT         1511 a          989 c          633 g         1695 t
ORIGIN      
        1 cttagagcac ttcgttatct atttatttct tcatttggct tatctctata atctcatgat
       61 ttggtccgta gaaattaaag taacggattc cattttccca gaacggtaat tccataatat
      121 ttttatctaa taattcatat cctaaatctt gaattgaatt gaatacctca tctatgtcat
      181 ccgtatctaa tgctagatga tcaatggctc ctctacactc agtagtttta tcttcccata
      241 tttctattat cgttccattc aactcgaaaa agccgacttt acttcccaca ttcatttctt
      301 cattaatcag actaaaccct aaggattcat aaaagatttt tgaagctcca aaatcttttg
      361 ttggaattcc aatatgttgt aaaccattta atttcactta catcatccct tcaattgttt
      421 taaaatccac attcataatt tattgaatag tctagtttat ctaccttgat tgctccctcc
      481 gtaacagtca tacttaatgc tgttccgact ttatcataca ttctagtact taaagcaaca
      541 ttatcaatat ataaaacagc tatcgtatca tcaacaatta actttagttc atattctttg
      601 cctatttcca aaaatagtgg tcgttgcagc cccttatcaa acatttgagg ccaaggatag
      661 ttcggagttt ttccgaaagt aagctgatta ttaatcaatg aaatacgaaa ttcgtatcct
      721 tcttgagtat cactatctac aaataatttt aaagcaaact cctttgttaa cgatgtaata
      781 gtcaaagttg tttcaaatag aaaataatct tcgattttag gaacaattac tctttctttt
      841 tttccaaatt ctttctctaa cttaaaactt gaaaatggag tgatttcttt aaaattctca
      901 attatttttt caggcagttt aacaccaaga gttttgtctg tcttttgaat aatttgatgt
      961 ggaacaagtg caccgcccca atcccagtta ttttgatcgt tctccccttc ttttgttgcc
     1021 acccagccag ttagaaaacg gccagtttca ttagttccga ctgttcttgc tgcataatag
     1081 gcttttccgt cgaaagcttc gtctactggt gatttccaat ctgaaaataa tcctgctccg
     1141 actcgatatt tagtcttttt atcttcacaa tactctgaaa aaagtaaata ccacgttcca
     1201 tcaatttcaa atatatcagg catttcaatc atgttaaatt cattattagc ccagtagtct
     1261 cctttgaaat tccaatcttt taaatcttcg gattcaaaat agaccaatct ccccgtcggc
     1321 tttttcttac ttgctggttt tctagctcct agtatcaaaa cgtattttcc atgatcttga
     1381 tcaaaaattg caaaaggatc gcgccaatca ttcttatcat atccttcttg aggaacaagt
     1441 tttgctgctt cgccatcctt tttccaatga attaaatctt tagaacttgc aatcattaac
     1501 acttctgaag tttttccatc caacttagct ttgcgattat gtccagtata caaagctaaa
     1561 tactcattga ttccttggac aatacttcca gcgtaaatgt attgatcaat gtcatcgttg
     1621 ccaccacgat gaattacttc cccgtattct tcaaatttca caaaatcagt ggttgtaact
     1681 agtgaccacc caaaaggatc agttaaaggt ttaggatttc tcgtatctcg ttgatgaaat
     1741 agataaaaag ttccatcttt ataaaaaggc atgcaatccc caaaccaaaa atctttaggc
     1801 ttgtaaaaaa ttttttgcat ttatcactca tcctcttcgt tagtatgtct ttgcatatct
     1861 gcaagacacg caattgtatt catatttctt agattttcga tgatatcatc gctttttttt
     1921 ccttgtaatc tcaaattgac agcctcaatc aaaattccta aattcatgcc accaagaagt
     1981 tgaaacccat tttgaaatac ttcacttctt ttagcaaatt cgttgaaagg tgttccacca
     2041 atcaagtcag caaataccat aacttcttga ttttttactt gagaaactat tttttcaatg
     2101 ttttcacgga attcttcaat gcctgcatct ccaagtgtta ccacatgaca atttaaatta
     2161 tcgccaaaaa acatttttaa tgtatctaca aatccaatgg caatatttcc atgagatgta
     2221 attataaact ccatccactc atcctctgtt cttaataaat aaaatccttt aaaaattctt
     2281 ttgaatctct aggagtttgt atcactgtca cttcaactcc atcactaatt agttctttaa
     2341 aagcctctct ctcttcttca gtaatattta tcgtcggacg gatgcgttct gttccattcc
     2401 ttcttgataa atttcctacg tttacacttg ttattggtaa accgttatca atcaaaaatt
     2461 taacatcgat tggagaaact gcaacaataa gaactttttg tccttcatat ttaccagaaa
     2521 gaatattttt aactgctact tcccttgtaa ttattgaagt tctaacatta ggtggtgcag
     2581 cgaggcgaac tacagctttt tttacgtcat cattggctac ttcattatta ataaccataa
     2641 ttctgttagc actcaattga tttgtccatt gtgttgctac ttgtccatga atcattcgat
     2701 catcaatacg aacatgtata atacctttca ttttttcatc catattcaac acctacttca
     2761 aaatatttaa cgctcttaac gcaatagaaa ctactaagat taatagaatt acataaactg
     2821 gtttaacctt tcgtcctaac aaataataca caattgctac tacacatgca ggtaataaac
     2881 caggcataac ttgatcaaag atttcatttc cggtcattgt taagtcgcct tgtttaaatt
     2941 caaagccaac attcactttt acaactgtgg caatgagtgc accgaccacc attaacccta
     3001 aaattgatac tgcatcagta atagacttta attttgtttc aatttgagtt agtaggctcg
     3061 cacccgattc tttcccagca aggtataaag gataagcagt aatccttaat aaaattgatg
     3121 ccagtatcca caaaataatt ccaacaaaac taccttccaa tgccatatta gctgcaattg
     3181 ctccaaaaat agtccatgga ataactacaa atacactatc tccaattccc gctaaaggac
     3241 ccattagacc tgttttcaga gcacttactg cttccaaaga cttatttttt tcttcttttt
     3301 caattccaac attcatacca ataatgtaag gcgctgtata cggcgtagtg ttaaaaaatt
     3361 gtaggtgagc taaaacggat tgtttcattc cttcaggatc atctttatat gtgtccttga
     3421 taaaaggaag catagaatac gcataaccca acccttccat tttttcataa tttaatgaag
     3481 ttgcaccatt gagaatccac cggaaaatta cttgtttgtc gatttttgtc agtttcttat
     3541 tcatcttcgt caaaacctcc tgttgcagct ggtgctactt gagtattatt tgatctattt
     3601 ttatatacga ttagtgcgat tgcagcgcca agtaacgaaa tccctaaaat tggtatttgg
     3661 aaataaactg ccaaaacgaa accaactaac aaataggcaa agtaatcttt tgtaggtaaa
     3721 tatttaagta gcattcctat cccaacagca ggtaatagtc cccctgctac ctttaaacca
     3781 ccggtaaccc attcaggaat ataattgatt gcacttccca ctaacgttgg gcctaatatc
     3841 acagttaaca aaacaggaat tccagataca aagcttgtat taattgatgc caaatattgc
     3901 atccactcta atttttgata atcttctttt tgtgcgaatt tttctgcctt ttgttgaaaa
     3961 aaaatgtttg ttgaccactt tagaacatct agttgtacaa ttaacattgc taccggtatt
     4021 gctaaagtta ttcctataga agcatcctgg ttagttgaga ttgctaaata agtaccgatc
     4081 aatgctgctg tttgataatc cggtattgat gcaccaccaa aactagtaat acctagtgtc
     4141 attaattgca aagtcccacc gatataaagt ccagtttgaa tatctcccat aataagtccg
     4201 gtcaagaaac ctgcaacgac aggttgggag gttccgattt tgggcccttc cttatcccag
     4261 ttaatgaaaa atccatacaa cacaactaaa aatgtttgcc aaaacgccat tttgttctct
     4321 cctttcttct tatactttta tataagcaat tactgtgcca actaacatta tccaataaag
     4381 cgggaaaata cagtaaatat taattaaatg agcgtctaaa tatactttgg acaccctttc
     4441 atttttttag acgctagtat gctatttaga aaatagtctg ctcaaaaatt gtatagatta
     4501 actcatactc tcctttgggt atttcaatac agtaaatatc tttgatttct gaaaaaaaat
     4561 cttttgatac atttaaaaaa gtatcaaata gttgcgctcc ctctttcaaa tgttcatttg
     4621 ttattccatc accaatcata attcgttcaa tcattccaga aagatgtaga aatagattaa
     4681 ttctcagaaa acttgggatt ttgacatcgt aatatttttc atacaaatat agtacttttt
     4741 ctatctcatt gaatactttt ttggaatcta aaaagcgaag cttagtacta gcgccttcaa
     4801 tagtaaagag tttaactaat tcatttaa
//
LOCUS       DASNNU010000136         4790 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.139, whole genome shotgun sequence.
ACCESSION   DASNNU010000136 DASNNU010000000
VERSION     DASNNU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.139"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..287
                     /locus_tag="U8116_002602"
     CDS             <1..287
                     /locus_tag="U8116_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435532.1"
                     /translation="KSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRFI
                     RMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            346..786
                     /locus_tag="U8116_002603"
     CDS             346..786
                     /locus_tag="U8116_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435533.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            798..1466
                     /locus_tag="U8116_002604"
     CDS             798..1466
                     /locus_tag="U8116_002604"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN8435534.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1530..2153
                     /locus_tag="U8116_002605"
     CDS             1530..2153
                     /locus_tag="U8116_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEN8435535.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2209..2595
                     /locus_tag="U8116_002606"
     CDS             2209..2595
                     /locus_tag="U8116_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEN8435536.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2808..4199
                     /locus_tag="U8116_002607"
     CDS             2808..4199
                     /locus_tag="U8116_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEN8435537.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1590 a          801 c         1009 g         1390 t
ORIGIN      
        1 taaaaagctt tttaaaagtg atcagtaaaa aacggaatct gatcaaaacg acacagctgt
       61 tgaaaaaata tccaggtctg cttcacacgt ctattcaatt tcctcaattg attacgatga
      121 tccatcgttt tatacgtatg caacagctac catctaagaa gctgtttctt ccattgacgg
      181 acacatttcg agtagtcaac agagaagata aaaagaaagt gattgtgatt gactttacca
      241 gaaatcggaa tgtccttcac caagttgcaa tggaaaaggc caattaaggc tttttctttt
      301 tggatttttt tgtcataata aatattagat gatggaggag ataatatgat gcaaacatgg
      361 ttagaaaggc taatacgatt tcagcagaac tatcacaaac ttatgcaggg aagatatgcg
      421 aaatttgatc agttgaatcg cggtctattg atcgctgcgc ttgtctttag tttgctaagt
      481 cgttggttgc cctatagaat cggacaattg ttgttcatcg tctttttcgg atggatgata
      541 tttcgtttcc tatcaaaaaa aatctacccc agactaaatg agaatcaacg ttatttgaaa
      601 tatcttgata gagtcaaaga aaaatatcat cagataaaaa acaaaaagca agtcaaaatg
      661 gatcgaaaaa gttatacttt ttttgagtgc ccgaattgtc atcaaaagca gcgtgcacct
      721 aaaggaaaag gacgaatacg tgtgacatgc aaaacatgtg gtataaaatt cgaaacgaat
      781 gtttaaggga gaaaagaatg aaattagtat tattacgtca tggcgaaagc gaagcaaatt
      841 ttgaaaatta ctggacagga tggctagatg tcgccttaac agaaaaagga aaagaacaag
      901 cagcagttgc cggtcaaaaa atgcttgaag ccggacttca attcgatgcc gtttatacct
      961 ctgttttgaa acgtgcgatt cttaccggac aagtcgcact agaagaaatg ggtcagttat
     1021 ggcttcctat aataaaaaca tggcgtctaa atgaacggca ttatggggat cttgtgggta
     1081 aaaacaaaga taagatgaaa gaaatatacg gaaaagatca agtaaaaaca tggcgcagag
     1141 gatatacgga agttccgccg ttaaccgcag aaaatagatt tgaccgcaga tatgatcact
     1201 tggatccgca tttgatccca tacggagaaa gtctggaaat gacagtgaat cggatcattc
     1261 ccttatggca agatcatcta gcaccaaaac taaaagatcg gcaagattta ctagttgtgg
     1321 gtcatggtaa tagcatccga gcactgacaa aatatttaga agatgttcct gaagatcaaa
     1381 tggatacgat cgatatccca aatgcacaac caatccagta tacattagca gatgatttga
     1441 caattctgga caaaaaaata ctttaaagaa cacctttatc taaaagtgag agatagaaaa
     1501 aagaagcaaa ggaatggaac aaaaggaaca tgaaaaatta tatatgggac tttgatggta
     1561 cattgtttga tacgtatccc gcaatggtag atggtgcatg gcaagcacta aaagattttg
     1621 gtatcagtat ggacaaaaaa gaaatctact tcaaaatgaa aaagtattct actagttatt
     1681 tgatcaatga atcaaacttg aatgccagag agtttaatga attatttcac cgatatgaaa
     1741 aagaaagtac ggaagtttcc cgtccatttc cagaaacgaa acaagtactg gaaatgctaa
     1801 aagacaacgg cggtcgtcat ttcatcctga cccaccgttt gacagaatca acatggggcc
     1861 tgttaaaaga acatcgattg gcacatctga ttgaggaagt agtagggatc gatcaggact
     1921 ttcctcgtaa acctgatcca gcatctttaa attatttgat cgatactttt catttagaaa
     1981 gaacagacac gatgatgatc ggcgatcgtc gtttagatat cgaagcaggg aaaaatgcag
     2041 gagtcgctac ttgtctttac gacatcgatc attttctggg tgagatcccg gcagattatg
     2101 tggtaggaaa tctgaatgaa atccttacct tgcagagcat cattaaaaag tagtatcccc
     2161 tttcaaagga gatatgttat aattttcata aaggagtgat aaaattatat ggaaatcaga
     2221 agacaaaaag aagttgtaga agcatttcat tatgatatga gattgccgaa ccaagaagta
     2281 gaaacagatt tgaaagtagg cttctcgcct ttaaaagaaa cgatcgaaaa ctatccgaaa
     2341 gaacatagca tcgtaggagc acgcttagag ttccgtttag tgtttgatac atatgtttta
     2401 tctggaagtg tgagtcagat caatcatatc ctcaatcgga aaattgaagg tcaaaatgat
     2461 gtcacacaag aagaagtgga cgagctagta gcaccgttat tcgatatcgt acgccgaatg
     2521 tcttatgagg tgacagagat cgccttagat cagcctgggg taaaattgaa ttttcaatca
     2581 gaaaaacaag aatagacagt aaaaacatac ctgcgataga ttaaagctat cacaggtatg
     2641 tttttttatt actaattgta agcaaaaaga atgtcatttc gataaaaatc gtctaaaata
     2701 taaaagtcac tgaaaaaaat tttttcagga ggatggttga agaatcattc tcgggaacct
     2761 ttacttcctt cgatggactt gtcattaata atatgaggag gagaaaaatg acaacactca
     2821 tcacagtttt agtggttcta gtttttgcgg cattattata tgtgttctat cgtatgcaaa
     2881 agcgccatac aaaattttca acacgcgtct ttacagcatt aggagttggt atcgtctttg
     2941 gcggtctgct tcaattgatc tttggtgtag acgacaaagt cgttactcaa tcaatggaat
     3001 ggatcgggat tgtaggtcaa ggatatgtat cattcttgca aatgctagtt atgcctttag
     3061 tgtttatttc gatcattggt gcattcacaa aaatgaaagt ttctgaaaag ctagggaaaa
     3121 ttagtgcgac tgtattgact tctttacttg gaacaacagc tattgcagca ttgatcggaa
     3181 ttggttcaac aatgctgttc ggactccaag gagctaaatt cacacaagga gctgcagaaa
     3241 cagcacgaat ctctgaactg gcaacacgtc aggaaacagt tgaaaactta tcgattccac
     3301 agcagatttt atcctttatc cctaaaaacg tttttgctga tttagcaggt agtcgtgcaa
     3361 caagtacgat tggagtcgtt attttcgcgg catttgtcgg aatcgcttat ctaggcgtga
     3421 aaagaaaagc gccaaaagaa gcagcattct tttcagattt agtcgacagt ctgtataaaa
     3481 tcattatgcg gatcgtgacg cttgttttgc gtttgacacc ttatggagta tttgctttga
     3541 tgacaaacgt tttggcaaca agtgattttg cagctatcgt caatttaggt aaatttgtcg
     3601 ttgcttctta cgtagcattg cttgctgttt tgattgtcca ctcactgatt ttgattggtg
     3661 caaaagtcaa tccaatcaac tatttcaaaa aagcctttcc ggtattgagc tttgcgttta
     3721 cttcacgttc aagtgctgga gctttgccat tgaatatcga gactcagaca aaagctttag
     3781 gagtagatga tgcaacagct aatttctcag caagttttgg tttgtctatt ggacaaaacg
     3841 gctgtgctgg cgtttatcca gctatgttgg caacgatcgt cgcaccagca gctggagtga
     3901 atgtctttag tttggaattt attttgatgc ttgtggcaat tgtaacaatc agttcttttg
     3961 gtgtagctgg agtcggtggg ggcgcaacat ttgcttcact gatcgtttta ggggcaatga
     4021 acttgccagt agcaatcgtt gggttagtta tctctgttga accattaatc gatatggcac
     4081 gtacagcagt aaacgtcagc ggtagcatgg tagctggtat cgtgacaagt gcacgcatca
     4141 aagatttaga tcgcgatgtc atcaatgatg attctaaagt gatcgatgtg aatgcatagt
     4201 caaaaagagg ttgtgataga agtggtcagc tttaagaaat aagaagaagt ttcctaaaaa
     4261 tagtttctca aatttttgtg aaatttcggc ttatttccga gaagctactt ctgaaacacc
     4321 gtgaataaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa
     4381 atagttcctc atattttacg tttggttgag cttattaccg cagatgcaag cttttgaaca
     4441 ccgtttaaga aagaggtcgt gagcctgccg tttcactctg agtcacaaag agcaatttca
     4501 aaaaatagct tctcatgttt gttgaatatt gtgtcttgtg accgaggagt tgtcaggaga
     4561 acaccgttta ccagaggttg tgacttgttt ttagtcacga cctctttttt tatttattcc
     4621 atataaatac acattattta ttccgaaaag tgaaagcgca aataacaaaa acaaccaact
     4681 tatttattat taaagaaaag cgtatttaag taggacgatt ctgttgagag ctttcgtaga
     4741 ttttcccatt ttcctatggt aaactgacaa agttgagaat gttagaacag
//
LOCUS       DASNNU010000137         4415 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.140, whole genome shotgun sequence.
ACCESSION   DASNNU010000137 DASNNU010000000
VERSION     DASNNU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.140"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            680..1648
                     /locus_tag="U8116_002608"
     CDS             680..1648
                     /locus_tag="U8116_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN8435538.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1659..2474
                     /locus_tag="U8116_002609"
     CDS             1659..2474
                     /locus_tag="U8116_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEN8435539.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2542..3252
                     /locus_tag="U8116_002610"
     CDS             2542..3252
                     /locus_tag="U8116_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN8435540.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3288..>4415
                     /locus_tag="U8116_002611"
     CDS             3288..>4415
                     /locus_tag="U8116_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN8435541.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1483 a          660 c          921 g         1351 t
ORIGIN      
        1 ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat gtctacatct
       61 gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt ttctgaatat
      121 aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct tttatttaag
      181 aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac tttttaatca
      241 gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata aagcatttta
      301 agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt ttccactcca
      361 gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc aagacaaact
      421 tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc gaaattagta
      481 tatgtactca agtttcactg cagattattt atatttttta gagaaataaa tttttcctat
      541 tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta tatgacgtta
      601 tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac atttttaaaa
      661 ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg tagaaaaaaa
      721 tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg tagatatgta
      781 tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct tatatacagc
      841 gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg tcattctatg
      901 ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc aagctagagg
      961 agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg tttctgatca
     1021 tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta tggccatcat
     1081 tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt acggtaaatt
     1141 cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc aggtgcagga
     1201 tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata tcttaggtag
     1261 tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg aaatgcaatg
     1321 gcttgattca acttctgttc attctggaga attcttccat ggaccttttg aagtaacaga
     1381 caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt tagatgaacg
     1441 ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg caaaggaact
     1501 aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt tattctacag
     1561 tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt tagatgttcg
     1621 tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta ggcttaggtg
     1681 ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga aattcattga
     1741 attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga gtttttggct
     1801 cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg gacaacagtc
     1861 actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat gagggcgacc
     1921 ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt cagcttactg
     1981 aagaagacag agaatattta aaccagtttg aattgattca tatgggatta tatagccatg
     2041 tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat gatttttctg
     2101 acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt ggcttctttt
     2161 ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat ttttgtgagc
     2221 gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat gatggagcaa
     2281 agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca gcgggagatt
     2341 ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc accgcgatga
     2401 aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc ggctttggta
     2461 ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga aagaaatccc
     2521 ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt gtacttacaa
     2581 ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa tacgaaaatt
     2641 cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt gagaaaagca
     2701 gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg aacttttgtc
     2761 aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga gagttgtaaa
     2821 tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc agcaactgaa
     2881 gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat ccagagaaag
     2941 cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta tgaaagatat
     3001 agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa agtccaaggt
     3061 accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga tgaccaaatt
     3121 gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac tttaatcaac
     3181 gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga agtatatcgt
     3241 ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg aagcaaatat
     3301 tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat attgtaatca
     3361 atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta ttaaatgaaa
     3421 acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat gataatcatg
     3481 tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat acatcttcag
     3541 ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca gatgaaccaa
     3601 tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa gagctattga
     3661 atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc cattgttgga
     3721 tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt gctgaaagtc
     3781 gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag gtttacgctg
     3841 aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc gtttttgatg
     3901 acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta tatgtgcagg
     3961 cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa gttttccctg
     4021 aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg gatagaagtg
     4081 gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac tatgaggcaa
     4141 ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg acaacggaag
     4201 gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag cggttacctg
     4261 aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca gcagaaaaaa
     4321 tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg aatcaaacgt
     4381 tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DASNNU010000138         4413 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.141, whole genome shotgun sequence.
ACCESSION   DASNNU010000138 DASNNU010000000
VERSION     DASNNU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.141"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            276..1007
                     /locus_tag="U8116_002612"
     CDS             276..1007
                     /locus_tag="U8116_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN8435542.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1152..3179
                     /locus_tag="U8116_002613"
     CDS             1152..3179
                     /locus_tag="U8116_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN8435543.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            3287..4384
                     /locus_tag="U8116_002614"
     CDS             3287..4384
                     /locus_tag="U8116_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN8435544.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1547 a          718 c          913 g         1235 t
ORIGIN      
        1 ttttacactc tcgtcaagtc acattttgat agataggagg gggacttacg aattatttta
       61 aaggctatga cagctgtatt tctagattgt gtttgcatca agttgcaata aaaactagaa
      121 atcaaatcgg gctgataatc atttataact aatcgataca taggaccaaa tataggtcta
      181 tgttctttta tttcataagt atcgtaaaac agaattttgc tatggattaa tccaatcgac
      241 tgatatgttt agtaaataaa gaaagaggaa tgtcaatgat gaaaaaagaa atcgtattaa
      301 cagcagcaat gatgttattc agcatggtag caagtacgac ttttgtttct gcaactgaag
      361 tatacccaaa ggaatataac acagaaggaa cgattacatt tgaagcagga gatgagggtg
      421 taactcctcc tgttgatcca gagaatcctg atcccaataa acctgtcgat ccatcagatc
      481 ctccaagccc tggaacagga ggggcattat cgattgatta tggatcaaaa ttcaaatttg
      541 ggactcaaaa aatttctact gcggataaaa cgtattatgc agctgctgat gtgatgaacg
      601 atgggtctag aaaaccgaca tatgtccaag tgacagatag gagaagtacc ttatctgggt
      661 ggaaattaag tgtctctcaa ccagaacagt ttaaaactgc cagtggggat gaactagttg
      721 gagcacagct gaaatttacg aaagggcagg ctgtttcttt ggttgatcca acatatacgc
      781 cgcaaacggt gaattctgaa ttaacgttga caccgggagg aaacaatacg ttagcaatta
      841 atgctaaatc aggaacaggt gtggggactt gggtctatcg atttggtgca aatgaaaatg
      901 aaaatcaaga tgcggtacag ctttcggtac ccggtaaatc tgtcaagtta gcgcaacaat
      961 attccactaa attagtttgg acattagaag atacacctaa caactagttt actgaaaaga
     1021 actgttaaaa ataaatacgt agtcatattg tcttgcgagg taatcgagca aaaattgtca
     1081 aaggctaggc taaatttgtt tggatacgaa tagttcatct aggaatttaa cgatcaggag
     1141 gaaaagatat attgaaaaag aggaggatcg tgctcatttt aagcagtctc ttagtgacta
     1201 atatctttcc gatcataggg tactctttgg aattgaatca gttggagcat ggaaatagaa
     1261 gagacgaaga aagcaaactc acttcgacat tcgacagttt aatagaatcc aaccagacac
     1321 aagaaagcag tgaggattta agtatttcga gtagtggaga gaccactgac aacgtagaag
     1381 ctgagggttc ggacaaacaa ggactagaag atgcaccaat ttctgcggtt gagctgcctg
     1441 acgatcccaa tattatttct atcgataaaa tttttcaaac acctatcggt actagcacgt
     1501 ccatattgga aaatgggaag ttactgcagt tgaacccagc agaaaggtct caaagaggag
     1561 ctatttggtc aaataagaag attagtttac tctcggattt tacatttaaa tcttatttat
     1621 acttaggaaa tcaacgagat agagcgggag atggcatgac ctttacattg actggcgatc
     1681 ctagaatggc tagtaatccc agtcaagtta ttggatcgcc aggtatggga attggctcat
     1741 attctactcg ttctggccaa ccttacattg cgaatgcgct ctcgattgag tttgatacgt
     1801 ataaaaatag tggttcttct gatcgaatgg atcgagagat aactgctgat aatagagggc
     1861 atggacatgt cgcttttgtc acgcctaaac caaataataa taattattct ggagagcata
     1921 ctggcgtgac ggtagctcct acatttcttt ctaatggcac atggcgcttg ttaataatta
     1981 aatgggacgc ggagcaaaga agtcttactt atgatttgga aggtgtcggt cgctcttctc
     2041 ggattatacc tgatctcatt aggcagtttg gttctacaga agtaaattgg gggtttactt
     2101 cttcaacagg tacttttttt caagaaaatg ctgtcgctgt cacgcgactt ccatcgaatg
     2161 tcacatcaac agctgatgta aaagtcaacg atagtgatta tggggccatc gctgaagctg
     2221 gaggaaacga taagataact atcagaaata cattgaaggt cgagggggat tttcttgaat
     2281 ataagaaaat gcagactagt atcgacctcc cacaagaatt aactattccc gagcatatct
     2341 tgatcaatgg aaatcaaatt gatgccaaag atatcttgat aaccggaaat cgagtcacgc
     2401 taaatctgac agattatttt attgctggag atacgttaat aatcgaattg gagacaactt
     2461 tgcaggaagt aattcctgaa aaagtactca ctatgcattt tgaatacctt gaaaacggga
     2521 atttgattga gcaatcgaat gaaataaacc taaagatcgc aaagctgaag gagatagata
     2581 tccatgttta ttataaagat aaagacagtc agattgatat tgctgaaatg aaaacaatca
     2641 ctgggaaaat aggagagaag tataaagaaa ctcctattga tattgaaggg tatgtttttg
     2701 actcggattc agcgaataca gaaggcatct tctccgaaaa tacagaggat atctattttt
     2761 attacagaaa aggcaaactt tatctctcaa ctgctcctca atatgtcaat tttggaaaac
     2821 ataaaatatc cagtagacca ttaagtgaat tcggacagtt ttcaggtggg ttgaaaatta
     2881 tagatgaacg ggcttttgga agttggaaat tacaattgaa acaagagaaa gttcttacaa
     2941 atggagattt tgagcttcca gaagctcttt cctttgttac ctctgaaaat acaacggtta
     3001 tagggacatc cgctgttacg attttcgaaa gtgatcaaaa gggtgaaagc gatttgtcct
     3061 ctcttttaga ttctacagct catcgtggaa tcagaataaa tattcctgta gaaaaccaaa
     3121 gaatcggaac ctttgagggg aaactatctt ggatacttgc agatgtccca ggaaattaac
     3181 aggaagagaa gaatataaca aaattattga gtttcaagaa gggcacaggt accataggct
     3241 cttgtgtccc acagagacaa gattttgtta tagtcttctc ttacatatga ttaaaggagc
     3301 gattttcttg agaccaaaac aacaattatt tataacactc ctagttattt taggggcact
     3361 ttttaacaat atatcagtgt cagctagtgc atttaatttt tcagtgacgc cgataacttc
     3421 agagaatcaa atcgacaaga gaaaaactta cttcgatctt caacttgatc cggatcagga
     3481 agtagaagta aaagcggaat tgagaaatga tacagaaaaa gaagtgaaaa tcgatatttc
     3541 tgtgaatagc gcaaccacca attcaaatgt gatggttgaa tacggaaaaa acgagattga
     3601 aaaagacatg agcctgatct ttgatctggt agattatgtg agctatccac agatagtgac
     3661 gctaaaacct aaaagtgtcc aaaccgttgc ttttcatgtc aggatgccga atgagcgatt
     3721 tgatggtgta ttggctggtg ggatcacatt ccaagaaatc cagacggaaa aggaccaaac
     3781 agaaactaag gatcaaagct tatcgattga gaatgaatac acctatatta ttgctttgtt
     3841 gatgcaacag acactgaacg aagttgctcc gaaccttact cttcatgaag ttaaaccaga
     3901 tcaaatcaat gcaagaaatg tcatcttagc aaacgttcaa aatgatcaaa aaacctatat
     3961 caatcaagta gttattgaaa caaaaattac aaaaaaagga cattctgaag ttttatatca
     4021 agaggaaaaa gaaggattgc agattgcgcc gaatacaaat ttttcttttc caactgcttt
     4081 aaatggtcaa ccgctaaccc caggtgaata tcaccttaca atgactgttt taggaaatga
     4141 aaatgaaaat ggaaaatttt cgagaaaaaa aggaaatact acgatcaact ataccaatca
     4201 atgggtattt gaaaaagatt ttacaataga cggaaaagta gccaaggaat tgaacacgac
     4261 ggatgttacg attaaaaaag acaataattg gatttatatc ttagtagctt cacttttctt
     4321 attgtttatt ttattatgca tacttttgtt tgttttgaga aaaaaagaag agaaagaaaa
     4381 gtaaggttaa gataacaaat agtaaatttt tat
//
LOCUS       DASNNU010000139         4411 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.142, whole genome shotgun sequence.
ACCESSION   DASNNU010000139 DASNNU010000000
VERSION     DASNNU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.142"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..859)
                     /locus_tag="U8116_002615"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="U8116_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="U8116_002616"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="U8116_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEN8435545.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="U8116_002617"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="U8116_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="U8116_002618"
     CDS             complement(2603..3421)
                     /locus_tag="U8116_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN8435546.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="U8116_002619"
     CDS             3582..4271
                     /locus_tag="U8116_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN8435547.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..>4411
                     /locus_tag="U8116_002620"
     CDS             4285..>4411
                     /locus_tag="U8116_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HEN8435548.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYP"
BASE COUNT         1462 a          725 c          779 g         1445 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca t
//
LOCUS       DASNNU010000140         4390 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.143, whole genome shotgun sequence.
ACCESSION   DASNNU010000140 DASNNU010000000
VERSION     DASNNU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.143"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(747..1040)
                     /locus_tag="U8116_002621"
     CDS             complement(747..1040)
                     /locus_tag="U8116_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEN8435549.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1443..2621)
                     /locus_tag="U8116_002622"
     CDS             complement(1443..2621)
                     /locus_tag="U8116_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN8435550.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2725..3039)
                     /locus_tag="U8116_002623"
     CDS             complement(2725..3039)
                     /locus_tag="U8116_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8435551.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3146..3361
                     /locus_tag="U8116_002624"
     CDS             3146..3361
                     /locus_tag="U8116_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435552.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3661..3903)
                     /locus_tag="U8116_002625"
     CDS             complement(3661..3903)
                     /locus_tag="U8116_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8435553.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3890..4318)
                     /locus_tag="U8116_002626"
     CDS             complement(3890..4318)
                     /locus_tag="U8116_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN8435554.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1387 a          834 c          653 g         1516 t
ORIGIN      
        1 ttttctcttt ttattctaag gttattttag tcttgtcctt taatacaaag attttatttt
       61 taaataaaaa atactctttt tttacgatta ttgaaaaaaa aagataaaac ttgattaatt
      121 gaagtattgc ttaaatttgg aagctatgag ggaagaataa aagagttagg acgggattat
      181 ctactatttt tctttgtttt aaatatttgt tatctttgaa cataaaagag aaatgtgtcg
      241 actgcagtgt tgtgtccctc atcacgaata aacggtgttc aactagctga tcttgtattc
      301 acttaaaaaa tggtaaagta cgagttgcta ttccccattt tcaattttct aacgctcagg
      361 acaaaatact gttttcacaa cctcattgga ataaaagcat atatattgtt tcaagaacga
      421 aatcttgtta tctttttaaa gggcatctgt ccaaaatcta attttaggag gtacgttttg
      481 gtatttattc gtacaaataa aagagaatta tctttattca tgtaacgcat ccaattgatt
      541 ttttcatgta cgctcctgga acgattttac tgacgattgc ttatttaact gcgaatcgct
      601 cattagcttt tatcatagct atccatattt tgaataacgt tcttggcttt gtattataga
      661 tacagatgac aaaaaaagtt caagtatgcg gtgtagaacc aatatcgcat acttgaactt
      721 ttttcaatga gtataaatta aaacttctaa ttttcaattc ccatcatctg atatgcccga
      781 ccgtctgaac ttcttttgaa aaagccaaat gtgattagat aacgtctttg ttcgacataa
      841 tcgccactag aaaatacgtt cagcagtatc gaattaacct ctgtttctgt ataagtcact
      901 ccatggtcaa agcgtttcaa gacttctttc aataggatgt atttcttttt tgttgaactt
      961 ggtacttgtt ttacttttcc atcattcatg aatgtgtcta aaactttcat cttttcttgc
     1021 tccgtataag tataaaccat cttctttctc cttttgattc taagaatact ccatttatgt
     1081 tagaatattc tggatttttt attaaagcgg ttactcttaa gaaagatatt atcatagtgc
     1141 tctttttttt actactactt ataggtagat ttattctaat ccattattct cccctaccaa
     1201 taattaaaga aaaaactgga tcagggtgtt caaattacat gctaagaaaa aagaaccctt
     1261 gaataccaag ggttctttct cagtctgata acaatgatta acgacggatt tctttgatac
     1321 ttgtgaaaag tcgttaaagt attgatataa aaggattttt attacctctg actacatatt
     1381 gactaatttg ttaaaaaatc gtttttcaag aatcctgtaa taccagtgct ttcaccgttt
     1441 ttttaactct ctaaagaact aaaagcaatc agaggtttta ccttttcatt tgacgttaag
     1501 tggcgataaa ttttcgaagt tgtttggtca gtatggccta agcgatcttt caagacttcc
     1561 cagctacagt gttccgctac taagcttgcc attgtatgac gagctaaatg agtatgaaat
     1621 ggcttattaa ttcctgagcg attcacgcac cgtttgacgt gatcatttac atactctcga
     1681 cgataaggaa caccacgttt actagaatca agatacacaa acaaatatgt ttcttctata
     1741 ataaacctgt aatgttgcct catctcaccc aaagccttaa tcaactgttg tagcttttcc
     1801 ataactagag gagttactag aactcgtcta gcagaactaa ctgtcttagt agtttcaatt
     1861 cgaaaatcct tactcttacc tttaactgcc gtttgcttat agaacagaat ttcatttctt
     1921 ttgaaatcta tatctccagg ctgtagagca agcgcctcgc tgactcggca gccagtgtaa
     1981 aatagaacat ggaaaagtac agacatcggt aattcctcat gaacaatgcc ataattcaaa
     2041 aaagttctca attcatcgac agtaagaaac ttgtcctcaa tattatttat ggcttctttt
     2101 aaatcagtta ccgttaactg atattttggc attctcacag acttcattgg attttcactt
     2161 atcaaattct ttctttgaca atatttaaaa atcatattta gaacttgaat aagtgattgt
     2221 aaactatttt tggcatagcc aacatttgtt ccattaacgc cacattcctt taaggtaaaa
     2281 agcaattctt gaaggcactc tggtgtcaaa tcagacaact ttttatcacc aatccatcga
     2341 acaataattt ttaattgttc ctctcgctta tcataggtta tttccttttg ttcatttaac
     2401 gaacggtaat attggaacca ttgctcacct acaacactaa acttgtcatt gcttgcaaca
     2461 atttttttct taagcctttg ttgtttctcc atttccagat aagcgagttg cgcctcacgt
     2521 tttgaattaa agccacgtct tgttgttcga tatgcttgaa agtaccatgt gatcctacca
     2581 gtagaactga tttgcttctt gaatcgatct gttttttcca tttattgatt cctcctattt
     2641 tgaaggaacc caacaagcag aaaccctttg gaaatctttc tatctatgtc atcattgtac
     2701 cgcatcttag aaagaaaatg taacttataa agatcgaagt agtcgcaatt tttctcgata
     2761 gtcattcgaa tcactctttc tcatccattc taaaacatca tcacgaaaat agtgtcctcg
     2821 aatcccgtac caacagggag gaaactcacc aagttttact aaacggttca gtgtactatc
     2881 acttatctga aatagtcgct ttaaatcagt atttgttaat accagaggta aatgactgac
     2941 tgcaagcaat ttttccgtgt aatcttccag ttttttttgt aaaattgaat agaactctat
     3001 ttccagagaa ttaacatttg ttttaacttc caactccatc acccttctct ctcaaaatca
     3061 aaacgaccac cacggtaccc cttaaaattg taaaaaaaca ctccagtact ccatcaaaac
     3121 ggactagaaa agaggaattt ttaatatgaa acaaggaatt gcagacataa aaataattaa
     3181 agaaattctt gaaaaatcta cggctaatgc aatagcattc ggaacaggaa tcaacttgag
     3241 tacagtaaaa aaacttaaaa gtggagagag agctgaagaa aaattaaatc ttgccgatgc
     3301 tattaaaatc acagaatttg gcatgaaaaa tatgccaacc aaaatcgaaa tatggaaata
     3361 gtagtttttc tgatctatca gctgttttgc caaagcacat aggaatctca cctcccccca
     3421 gtaatggcgg gccgatttgg ttacggaagt atcattatcg tagtcgcgat tctcatatta
     3481 aaatttgtcg cttaaaagct aaccttgtca tggcgtaact agagaatcaa aatctacatg
     3541 tactgataga tttgctattc atttgtcaaa gagcgctagg ctattttcag cctatggaaa
     3601 tcctttattt tgacccgata aaaaacttcc atagaatgaa aaggaaaggg aaaaaatact
     3661 ttaacgagtt gtatcaaatt caagtatctt ctcaatcaat tttgtttcca gttgtctacg
     3721 catatattca tctacataca taaccattcc tttttcttct tcataaactg gtttcatggc
     3781 taaccgtgaa atataatttt gatattttga cagaacgatg gacatggcaa ttgaatcacc
     3841 agaagctgcg gctttgattg tcgttagtgg tatgaatggt atatatcgtt tattttgctt
     3901 catatttgac acccaaatac tttcctagct cttccaaagc catttgtcgt tggtaccaaa
     3961 cactgccgag acttttccca ataatatcgc caatttcacg atcattaaga ccgatgaaga
     4021 aggacataaa aatgatttct tgtctttgac taggtaatgc tgcaatcgca tttgccaact
     4081 cctcattgga aataaagatt ttcataccaa ggacaataaa agtaccacta ctattcaaat
     4141 ttggttccaa ataatagaac tctgtaccta accctttttg ttccaatatt tccattgata
     4201 tttgccgttg gcgaaatcta gcgtacttct tttgaatatt taacgcctca ttgcagacta
     4261 ctttcttaca aaaactgtca aacgtatgtt gaatgtgggt ttcctctgag tcgcccataa
     4321 tattctcccc ctcaaaaaat tcattcaact cgttttactt ctatcccttt ccattattac
     4381 tacaatctag
//
LOCUS       DASNNU010000141         4338 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.145, whole genome shotgun sequence.
ACCESSION   DASNNU010000141 DASNNU010000000
VERSION     DASNNU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.145"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            153..1454
                     /locus_tag="U8116_002627"
     CDS             153..1454
                     /locus_tag="U8116_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN8435555.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1481..1741
                     /locus_tag="U8116_002628"
     CDS             1481..1741
                     /locus_tag="U8116_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN8435556.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2740..3975)
                     /locus_tag="U8116_002629"
     CDS             complement(2740..3975)
                     /locus_tag="U8116_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN8435557.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1269 a          686 c          888 g         1495 t
ORIGIN      
        1 agaaaactta ttttcaggat tcttaaaaat aaatttgatt atttaagaga atatattgaa
       61 ataaaatttc ttaattatat aattagttat gaacattctt tgcgttgtca tgcaatgaat
      121 ggttcatatt ttattgaaaa ggagtggttt tagtggaaaa gctgatgaaa tgggtcgaag
      181 agaaacttgt cccaccaatg gcaaaaattg gtacacagcg tcatttatta gcaattcgta
      241 atggggttgt ttcaacattg tcattgattt tgattgggac attttttatg gtctttatca
      301 atttaccgtt tcctggatgg aatgaattta ttgcccctta ttcggcaacg attgttttac
      361 cctttaggat gacgatggga ttgatggcta tttatgcaac ttttgttatg ggttcggatc
      421 tagctaaatc atatggatta gatagtgtga caggtggtat attatcttta ggaacgttct
      481 ttatgttaca agttcctgta aacgttttaa ctcctgaaga ggcacctctt ggctgggtct
      541 tacctatgag ttctttggga gcatctggga tgtttgcggg gatcttatct atgatttttg
      601 cagtggaagt atatcgtttc tttaaaaatc gaaatattac tatcaaaatg ccagaacaag
      661 tgccacctgc tgttgcccgt agttttgagg cattgattcc aggtgcagtc attttaacaa
      721 ttacttggct gattcgaagc gtcgcgggct ttgatgtaaa cgctgcctta ttatcgttat
      781 tcgagccttt aaccaatatt ttagggaata atttgcttgg tgttttacta ccaatgtttt
      841 tgatccattt gttgtggtca tttggtatcc atggcatgag tattgtaggg gctgttgttc
      901 gtccaatgtg gctgatcatg ttggatgaga atgcaaaggc attggcagat ggtacagctg
      961 ccacccaatt accctatatc gctcctgaac agttttatca atggacggta acaattggtg
     1021 gtgcaggagc aacgatcgtg gtatctgttt tgtttatatt cttctgtaaa tcaaaattct
     1081 tgaaagaggt tggtcgtttt tctattattc ctggtatctt caatatcaat gagcccataa
     1141 tttttggagc tcctatgatg ttgaatcctt acatgttcat cccgttcaac ttagttcctt
     1201 tagttttgac tattatcagt tattttgcag tcaaattaga aatggtcaat agatttactg
     1261 ttttgccaac atggacatta ccagctccaa tcggcggtta tctttcagca ggaaatgact
     1321 ggcgtgttgt tatcttgatc atgatcaaca cattgatagc atttattatc tattatccat
     1381 ttgtaaaagc ttatgataaa aagatgctgt cagatgaaca aagtcgtgga gaaaacgaag
     1441 tccagactgt ctaagtttgg tttttagaag gtgggaaaca atgtattggc tgattttatt
     1501 ttttgttttt atctttttat taacggctag tcaccttata ttgaatatgc tagctacata
     1561 tcatattcaa atcaatcgat ggatttgggc actggcatca tttttgatcg tgattcttcc
     1621 aaagatcatt gtaccgcata tgaatgtttt gttttcatgg gggacatatg tactttgtgg
     1681 aatttttgct atcaatttta tgatcgagca gcatagatgg tttgtcacat cgaagcttta
     1741 aatgttctaa aagtggttgt aacagaagtg gttagcttca agaaataaga agaaatttac
     1801 gaaaattgct tcttaaattt ttgtgaaatg ttggcttatt tttcgtttgg ttgaacttat
     1861 taccgcagat gcaagctttt gaccaccgtt tattcggaat atgggactgt gacaaggctt
     1921 ttgtcacagc ccttttttac ataaagatta ttattgtttt aactgttttg gcagaaagaa
     1981 ggaggaaaaa atggaaattt tatcagcaat ggatttagaa ttagtagatc tactaccaaa
     2041 gtgatcaagt caaatataga tttgggagct tacaaccata tagtaggggc tgcagtatgg
     2101 tgtatgaatg gtaatatgta tgatgtgagt gtttattcaa atcatgaaac gtgtatagaa
     2161 ataattgtgc tgggaatagc aatcagtcat ggtgaacgtg acttttagtg tatcgttgct
     2221 gacgacatag aacattctga catgatctac tcaatatgtg gggattgctg gcagtttata
     2281 taggagtatg catcggattg cgagatcatt gtctcaacag aagagggagg ttgattatca
     2341 aagataaagt ggtaaatcct acatttgatt tagtaattgt tgaggggact agcggcacaa
     2401 atctctacaa agcgatgctt ctagatctat atgacacatt ttaagaaaat gtatttgcct
     2461 aattattatg gtctttcttt tgaagaaacg ttgagatgat ccaaccagag aaaggtgtca
     2521 aagaatttga ctaagaataa atggcagacg ctacctatgc tcatttggat agcgtcagat
     2581 cttttatttt aaggatgatt ttgtgttttt tagacaccgt aatttaagta actctaactt
     2641 ttcaatccac tatttacttt ttttgtattt tcactgtctg ggtctcgaat agcgaaatta
     2701 ctgatgagtc tctattaaaa aagctttttc tacttgaaac tatattgggt ggttgctcag
     2761 gaaggtttcg atttcttgaa ttgcttgttg tggaccattt attggaaaaa cggcttgcag
     2821 cgtgcgtgcc tgctgacgaa tcttgggtga agaaacttcg cgcagagcaa tctgcagttt
     2881 tctttcagtc actggttttg tgagggcgat tgcgtttccg tattttacac aatagtcaat
     2941 attacagctt tgctcaaatt gcatccccac accgataaac ggtttgccgg attcacaggc
     3001 agtttggacg gtcccttccc cgccgtggat aaaagaaaaa tcaatgaatt catgcaaaag
     3061 atgactagac agatattctg ttattaaaag atcttctgag tcagattgaa tgtcatcaag
     3121 cagatagtca ataggtgcaa taaccgctag atccggttgt ttttgtaaga agaacaaaat
     3181 tttgcgtaac atacgcggat tgcctgaact acccattgcg aaatagataa tacgttttct
     3241 ttcttgttgc tttcgctgta taaaagttag cacttcttca ggcaaggaag tactgagctt
     3301 agcaaaaatt ggaccaacga ttcgataatt ttccggcagc tgttcttctt ccaaaaatag
     3361 tttaggcgtt gtgatgagat ttagatcagc atccatcatt tgtaaacttg tttttggtaa
     3421 aggaagcatt tctttttgtg cagcgtgttt gaaggcattg gggatccatc gggtatttac
     3481 tatacgcttt tttgtccagt gccaaagggg attgcttaaa gggccgaaga aacgtaactc
     3541 gtggggcatc agcgcatgct ctgattgaaa aaaaggttca ctcaaagcat agggtttaac
     3601 atatactaaa ggaatctttt tattgcgggc cgataaaaaa attgtcacat tcgaacctgt
     3661 cacaatcagt cgaggctcaa acttagcaat cagactctgt tcactagtga tacgagaggc
     3721 aaccatttct ttggtaaaag ggtttttaag tgtacgcaac tggtcaaatt ttatgatttg
     3781 ttcttcttgt tttttcgtaa ggatcggcgc tagtaaatga tactcaaagc cactttctgt
     3841 gatcaaagga gcaaaagttt tagaaaagcc aaagaataca cagtaataac ggtctttcat
     3901 agctttggca acctcaatca ttcgtgttgt ctcagctaaa ttgaatactg caggagcaaa
     3961 aagaattgtc ttcatgaaat cctccttatt ggaatgagtc tatcccttcg tttcttacta
     4021 gtatactaaa aaacacagcg ttcgagtagc ctatgtatat tttgaatatt aaactgtctc
     4081 tcgctgtttt cacaacttct aaatttaaat tttgtaatca agttgaaact ccaattttca
     4141 aaaaaacacg caacaatttt ttgatcgatt tatatgttgt gccagataaa tacgagaagt
     4201 tgtgtcatta aaaataaaca tgagaacata aaaaaataaa ataattatat taattgatag
     4261 gtgtgctgtc gtcattatac acacgtaaga aagaaatagt tatgtttaaa aatattctta
     4321 gactttctcg ccatgaca
//
LOCUS       DASNNU010000142         4317 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.146, whole genome shotgun sequence.
ACCESSION   DASNNU010000142 DASNNU010000000
VERSION     DASNNU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.146"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            117..554
                     /locus_tag="U8116_002630"
     CDS             117..554
                     /locus_tag="U8116_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435558.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            649..1533
                     /locus_tag="U8116_002631"
     CDS             649..1533
                     /locus_tag="U8116_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN8435559.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1755..>4317
                     /locus_tag="U8116_002632"
     CDS             1755..>4317
                     /locus_tag="U8116_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435560.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1410 a          723 c          964 g         1220 t
ORIGIN      
        1 ttagttcttt ttattttaaa aatttttttt acttagaatc cagccaccct agatattttt
       61 ctgtataatc ttaaataaag aaaacgatat taaaaaatgc acgtagaagt agataaatgg
      121 agtggttttg caaatttctt ttatttctag aagttattca cgtatacgga tttgtttttc
      181 tcacaaataa aaaggcagga agtacaatgg atcatttgta tataaaatat attcatcaat
      241 tttccgtttt ggtattaggc acactaatta tagcggggat tgttgcattt attattcaag
      301 gtcgtgacat attaacagta catcaagagt ttttcagtta tgcatttggt attcctctga
      361 tcgctataag gtctgataac aaaaagtgga agaaacgcta ttacattctc ttgctatttt
      421 tgtgttttta tctgccacct aagattttta ctttattagg tatacaatgc ctatcatttt
      481 cttttatttc ctatatcatt gttttaattt tcctcagttt catagtatgg gtattttatc
      541 ttgtcaaaaa ataaatgaat gtttcatgaa cagtacttca tcacagccta atttttttct
      601 ttatgctatc gttatattaa agaaatcaat cagaagggaa agaaagtgat gaaaaaagtt
      661 ttattagtgg taaaccccag ttcaggcggt gaacaagcaa aggaatttga gcaattagcg
      721 atagcaaaat tggaatcggt gtttgatgaa gtggtcgtct tgcatacaaa aaaagcaggg
      781 gatgcaaaaa actttactcg cgaagcggct acggaagggt atcatagtgt gtttgtcatg
      841 ggcggagatg gaacagtcaa cgaaggaatc agtggaatcg ctgaacaaga acatcggcca
      901 aatttcggat ttttcccatt gggtacggtg aatgacttgg ctcgagcatt agggataccg
      961 ctagaacctg aggaagcgat caatcatttt tcgattgaat cagtcaagcc attggatatt
     1021 ggaaaaataa atgacgacta ttttatgaat gtcgtcgcaa ttggctctat ccctgaagca
     1081 atcaatgatg tagatgcgga gaagaagacg aaatttggaa aactagctta tttcatgtca
     1141 ggaatcaaac agttagcttc cacacaatcc tactcgtttc acgtagaagt tgatggaaag
     1201 aaggaagaaa ttgagagcag tatcttgctg ataggactaa ctaactcagt aggcggtttt
     1261 gaaacattac ttccaaatgc aaaagtggat gatggtaagc tccatttggt ttatttaaaa
     1321 gattcttctt tgatcgatac gcttaaaaca ttgccagacc tattaaaagg agtagatgaa
     1381 tctacgaaaa atctagccta tcttacttgt gagcaattag ttgtgtcatt agctgatgag
     1441 gaagaattga cgacgaatgt tgatggagat gaaggggata aattacctgt gacaatcaaa
     1501 gtgttaccct cacatttaca ggtttattgt taaaatggag gttgtgaccg aagagttgtc
     1561 aggaggacat cggttgttcg gaataaggga ctgtgacaag acttttgtca cagccttttt
     1621 ttgtacaaaa aagaaatttt cacctaacag aaaaatttgc cataaaaata catattttaa
     1681 atatgaaagt acaattacaa ataaattaaa acgttaaaat aaaattgtca aaacgtcaga
     1741 gaggatggtg gagaatgaat caatatgtaa aaaagctgat tgcgtcgatt ttgtttgtct
     1801 tccctctctt gatggtgaca tccgtttttg ccgatgtaga gaaaacaagc gaatcaacgc
     1861 aatcagagaa tcaaagtttt gacaagacta ctttttcaga agaatcaatt acttctgaaa
     1921 ctacagaatc ccaaagtgag gcgcctactt ctaatacaag gcaagaagaa aataagacaa
     1981 gtgagacctc agaagtagct gaacaaacgt ctgatagcag tgaagaggta caggttagag
     2041 ttgtttcagc ggatgggtac agcgaagctg ccacaacaga agaactgacg cagcttctcg
     2101 cagacgaatc agtagctaag attcgtttaa ctcagccttt gactttagac agggaattgg
     2161 agatcaagcg agatatcgtt atcgattttg gcggacttgc tcataatttc ggcacacatc
     2221 acatctatat caatgagaca cctcagtcga tcgagtttca gaattttaaa ggaacggcag
     2281 ctcatccagg tgggttgata ccaaatatcg atggaaatgc cattatcatt gcttatatgt
     2341 ccgatttttg gggaaacaga tatcatttta cgggagaaat aaaatttacc gggacacttg
     2401 acttatatga tggttctaaa ttaggcctga tttacgcccc tcgtgcaact gtcacattag
     2461 atggtgtttc cggtgtattg gatgtccaac cagtgaaaga agggatgaat gccgctccgg
     2521 gtaaagctta tttttgtaga agctatcagc taaatgtaat caatggtagt cagctttatg
     2581 gaccatatct aggaaaattc tatggttttc tagattcagg tgatgagacg ggcagtggaa
     2641 aaacagcacc aggactccat atcttgagtg gttcaaaggt ctctcttgac tatgatcggg
     2701 cagatggcgt cagcgatgaa ggagaggctg tcgatacgct acccgggaat gtcacattta
     2761 aagtcagtgg ctcaggaagt gaattttcgg taaatacaaa gatcaatatt accaacgaca
     2821 acaatcgcgg aattatccaa atgagaggct caggatcaag agtcgctgtg tcgaatgatg
     2881 ggaaaatcac aatcaataca gcgactactg gcggatttcg cctgcaagga gattctagtc
     2941 aaatccatgt atcaagtggt ggacaaatct cggtcaaaat ggctggagat ggcgaccaac
     3001 ctggaaacaa tgggatgcgt tttgtaggga aaggactttc tttgatcgta gaaggagccg
     3061 gatcagtcat cgatattgat aagcaaagtg gtcgttctag cgcagttcgt tttgagaacg
     3121 gtacgcaaaa actaacagta actaatggag gctcattgaa ggtaagaaat gcaggggatg
     3181 gcaaatctta tgtgaacaga ctcaatcaag gaaaccaagc gatccaattt tatgacgctt
     3241 ctggttttct agaaagtcca gggtcagctg attttacggt tactggagaa aaatcaaata
     3301 tcgatatacg cgcagattcc ggagctaccg tagatacaac tggagatatt gatttgaact
     3361 ttatggctgg cgaaaaaact tatttagtca tggtcgggaa aactggggaa gataagactg
     3421 gcatcatcag tggggatgtg ttgaatgtag acttagaatc tcctacctat ttcgatttcc
     3481 aaaacaaacg gatcggaagc gagactaatg gcataggtgg ctgggtattt caaggaaatg
     3541 gcgattcttc tttgaagatc gctcattcag aaattgcttt atggcgaaag gcaggagtaa
     3601 cgttcgataa ctttgaaaaa gatccggatt atttttctga tatgacagac ttatcgatca
     3661 atggaaccga cttaggtaat tttgtatcaa gccctagtgc aggtttaaca aatgaatttg
     3721 ctcagacagg tgccggaatg aaaaattata atcgaatcag tgccaataac cagcttccga
     3781 tgattgaatc cttaagagta ccaacgaatg cagataaaaa aatctatggg catgtcgttg
     3841 ttcccgaagg tgtcgaggca aagccaagag atgcttggac aggcgaagta gaagtgactt
     3901 tacgtattac ttatgcagat acgacgaaat cacctgtaga aattactgct ctcacacaag
     3961 gacaaacaga tgaaaatgaa gggacaggat ataatccgtg gggagaaggc gaacaaggcg
     4021 gtttgtttga ggttcaagta cccaacgaag aaagactaac aacgggggat caagtgcaaa
     4081 tagttgctgc taaaaaagta agaggcggcc aagaagtgac agaactggac tctgccaaaa
     4141 cgacagtcga tgtgattcct cctgagcctg ctaaattaag taccacattg atcaatgcag
     4201 cttctcgctc aatcacaggt acaggcgaag caggagcttt ggtttcattg aaaaaaaatc
     4261 aagctgtttt gtccgaaaca actattgatg atacaggtaa atttgttctt tctatcc
//
LOCUS       DASNNU010000143         4305 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.147, whole genome shotgun sequence.
ACCESSION   DASNNU010000143 DASNNU010000000
VERSION     DASNNU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.147"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="U8116_002633"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="U8116_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEN8435561.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="U8116_002634"
     CDS             complement(2020..2922)
                     /locus_tag="U8116_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN8435562.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="U8116_002635"
     CDS             complement(2919..3611)
                     /locus_tag="U8116_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEN8435563.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="U8116_002636"
     CDS             complement(3654..4295)
                     /locus_tag="U8116_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN8435564.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1323 a          922 c          753 g         1307 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacc
//
LOCUS       DASNNU010000144         4218 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.149, whole genome shotgun sequence.
ACCESSION   DASNNU010000144 DASNNU010000000
VERSION     DASNNU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.149"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            11..733
                     /locus_tag="U8116_002637"
     CDS             11..733
                     /locus_tag="U8116_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN8435565.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            791..2830
                     /locus_tag="U8116_002638"
     CDS             791..2830
                     /locus_tag="U8116_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEN8435566.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2939..4090)
                     /locus_tag="U8116_002639"
     CDS             complement(2939..4090)
                     /locus_tag="U8116_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN8435567.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1535 a          782 c          816 g         1085 t
ORIGIN      
        1 attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg agtttatcca
       61 tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga aaagtatccc
      121 taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg aaaacggtcg
      181 ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc
      241 tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc
      301 ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga
      361 agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt
      421 tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa
      481 caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga
      541 caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca
      601 gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg
      661 tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa
      721 agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
      781 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
      841 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
      901 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
      961 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
     1021 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
     1081 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
     1141 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
     1201 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
     1261 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
     1321 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
     1381 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
     1441 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
     1501 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
     1561 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
     1621 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
     1681 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     1741 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     1801 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga
     1861 cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     1921 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     1981 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     2041 ttatgctcct ggatctacgt ttaaaataat tacagcagca atcggtctcg acaacggcac
     2101 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     2161 gggatctttt caaacaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc
     2221 tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa
     2281 atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     2341 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     2401 tggatatgga cagggcgaac ttctaattaa tcctatccag caagcagcaa tgtattctgt
     2461 ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     2521 taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt
     2581 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     2641 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt
     2701 cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     2761 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
     2821 ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt
     2881 ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc
     2941 acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta
     3001 ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc
     3061 caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca
     3121 atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg
     3181 tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa
     3241 aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag
     3301 cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa
     3361 aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac
     3421 aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa
     3481 gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt
     3541 caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc
     3601 tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg
     3661 atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag
     3721 aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt
     3781 tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac
     3841 caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta
     3901 ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca
     3961 atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta
     4021 atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa
     4081 taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca
     4141 ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg
     4201 gattgttggt ttttatac
//
LOCUS       DASNNU010000145         3991 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.150, whole genome shotgun sequence.
ACCESSION   DASNNU010000145 DASNNU010000000
VERSION     DASNNU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.150"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(105..1610)
                     /locus_tag="U8116_002640"
     CDS             complement(105..1610)
                     /locus_tag="U8116_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN8435568.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1607..2293)
                     /locus_tag="U8116_002641"
     CDS             complement(1607..2293)
                     /locus_tag="U8116_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN8435569.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARNVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2486..3907)
                     /gene="gndA"
                     /locus_tag="U8116_002642"
     CDS             complement(2486..3907)
                     /gene="gndA"
                     /locus_tag="U8116_002642"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEN8435570.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1097 a          849 c          687 g         1358 t
ORIGIN      
        1 tgttcgctag tttttttata atgacatccc tgaatttctc acttgcttgt attttgtacc
       61 ttattctttg ttggatatat aaaaaaacaa gtaaagcaca tgctttactt gtcttctgcc
      121 tttgcgatag gaataaagat tctaaaaatt gtcccttgcc ctactacact ttctgctaag
      181 atacgtcctt tatagttttc aaccagttgt ttagcgatag acaaccccag accatttcca
      241 cccttcgttc tagctcttgc attatccact cgataaaaac gattgaagat cttattaagg
      301 tcttctttgg gtattccttc gccaaagtcc tgaatcgcga tctcaaattc attcagagtg
      361 gaagaaatag agatatggac ttcttttcgt gtagttgaat actttactgc gttatccaat
      421 atgataatga ttaattgttc aaagtgatta cgataaattt gcagggtcac ttcttttgtc
      481 aaatcatcat ctaaagtgat cacatagtct ggatataata tcttgaaatt attataaact
      541 tgataggtga cttcttttcc tttagttgtt tcattcgcat aatgaacatc tacttgttct
      601 gctcttgata agtctagcat ctcttgaact aagcttttca tacggctgat ttcttgcatg
      661 ctggcgctta atgattcttc taatatctca ggatcatctt tcccccaacg atttagcaga
      721 ctaagatgcc cttcaataat tgcaacaggt gtccttaact catgagatac gtcttccacg
      781 aattgctctt gttgttcaat atacaaacgc atacggtcaa gcatctcatt gaatatttca
      841 gctaaatctg ccaattcatc atttgtgtcg atttcaggca tgtggatatc tgattgcgga
      901 tctttacgta tagtatccat tgtatctctg agtaccttga gtggttttaa gaaataagaa
      961 gataaaaaga aacctaaaat actacttaag atcaaagaaa caacttctaa aacaataaga
     1021 gtcaataata gcttatttcg aatatcatag aaagaagata attcatagaa gctttgcgca
     1081 tagccgatct tttctcttgt acctttagaa tatataggct ggatagataa aaagccagtt
     1141 tttccttcta gtgtaacgat cgttggaact ttacgtgtag tctgaatcag gtttcgactg
     1201 ttttcatggg ttttaaaaat caagttctcg tccaaatcat aaacataaag atccattgat
     1261 ggctgcccta gctcagctat aaaggaatcg atcttcatta agtttccttc gaccagtgaa
     1321 gtatgatcta cgacctcatc tccgtcattt gtcgctttcg tcagattacg atacgtattt
     1381 actaatgtca gttcttcatc cgagttagcc aaacgagaag ctacttctga aatcgtacgc
     1441 tctacattgt ttctttcttt tgccacaatc agattgatag atgatttata ggtaatgaca
     1501 gcgaaaattg taaacacaac gaatatgaag aaagaactcg caaaggccca cttgattgtc
     1561 aaggagggcc ctttcagttc tcttttttcc gattttttcg gctttatcac gagcgcatca
     1621 cataacctgt tccgcgtaca gtttggatat agctttcctc cccgggaacg tcaattttgt
     1681 tgcgaagata acgaatatat acgtccacta cgtttgtttc aacttctgtt tcgtatcccc
     1741 acactttatt tagtaaaacg ttacgtgcta ataccacgtt cacgttttcc attaaagtaa
     1801 gcagcaactc gtattctctt tttgttagtt caattacttc atttccgcga cgtacgacac
     1861 ggttttcttt ttcgatagtc aaatcgcgat aagtaatagt agtttgttta gccacatttt
     1921 tatcgccttc aatatcgatt cgacgcaaca atgcacgaag acgtgctaat aattcttcga
     1981 tagcaaatgg tttgacgatg tagtcatctg ctccgtggtc aagtccagat acacgatcga
     2041 tcacagaatc tcttgcagtc atcataatga ttggtgtatt tttgacttgg cgtacacgtc
     2101 tgcagacttc taaaccgttc aattctggga gcatcagatc caacaatatg gcatcccact
     2161 cattatttaa tgccgcatct aaaccagttc ggccgttgta gtggacttct gcgttatacc
     2221 cttcatgctt caactctagc tcgacaaatc gtgccaagtt cttttcatct tcaatgatta
     2281 gaatattgct catttattat tttatccttt ctattctact ggctttttaa aaactctatc
     2341 attataccga taatatgctt aaaaatctat agtcatttta tcatggatag caattttaaa
     2401 aaagagctga agtctactca taacaatcga cttcagctct ttcgtcctct ttttattttc
     2461 tcatttccta acagccgaaa gactattatt cttcatggta ccatgagtaa tggaagattc
     2521 cttctttgtc gacacgctca taggtatgtg cgccaaagta atcgcgttgg gcttggatta
     2581 tatttgcagg cagacgttct gctctgtatg aatcgaagta agcgatggct gatgagaaag
     2641 tcggtacagg tacgccagct tggacagcca aggcaacgac gtctctgact gattgctgat
     2701 attttttcgt gatttcaacg aagtaatcat ctagcaataa attttcgatc tcaggatttt
     2761 tgtcataggc atccgtaatt ttttgcaaga atcgagctcg gatgatacat ccagcgcgcc
     2821 agatttttgc gatttcacca aatggcaagt cccaatcaaa ttcttttgaa gcgacacgta
     2881 attgagcaaa tccttgggca taactcatga ttttactgaa atacaacgct tctcggattt
     2941 tttcgaccaa ttcttttttg tctcccgtga actcaaaatc gttcgtacga gacaaaatct
     3001 tgcttgcttg tacgcgttct tctttataag cagaaatata gcgggcaaat acagattcag
     3061 tgatcaatgg aagtggcaca cctaagtcaa gcgcgctttg gcttgtccat ttacctgttc
     3121 ctttattccc agctgcatca aggatcacat ctactactgg tttacctgtt ccttcatcgt
     3181 ctttacgtgt cagaatgtca gcagtgattt ctatcaaata gctgtctaat tcgccttgat
     3241 tccattcttt aaagatatca gccatttctt ctactgaaag gtttaaaata ttcttcatta
     3301 agtcataaga ttcagcaatc agttgcatat ctccatactc aataccgttg tgtaccattt
     3361 tcacataatg tccggctcca tttgggccaa tatatgtgac acatggtgct ccgtcttctg
     3421 cttttgcaga aatttgctct aagattggtg caactagcgc ataggcttct ttttgtccac
     3481 caggcatgat tgaaggtcct tttaaagctc cttcttcacc accggaaacg cctgtaccga
     3541 taaagttgat gccagagttg gctaactctt cattacgacg catcgtatct ttaaagaatg
     3601 tatttcctcc gtcaatcaaa atatcgcctt tatcaaggtg aggaagtaac tcttggattg
     3661 ttgcatctgt tgccggtcct gctttgacca ttagcaagat ccgacgtggt ttctcgatag
     3721 attctacaaa ttcttcgatt gtatatgttg ctttgaaatt tttatctggg tgttcttcta
     3781 ctacagcggt tgttttagcc cctgtacgat tgaataaagc cactgaatag ccgcggcttt
     3841 ctatgttcaa ggctagattt ttacccataa cggccatacc gacaacgccg atttgttgtt
     3901 ttgacatatg aactcctcct acatacggga tttctcccta atctcttaca ttatagctta
     3961 acacggctac attgcaaact aaagaacaac a
//
LOCUS       DASNNU010000146         3985 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.151, whole genome shotgun sequence.
ACCESSION   DASNNU010000146 DASNNU010000000
VERSION     DASNNU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.151"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2887)
                     /gene="brxC"
                     /locus_tag="U8116_002643"
     CDS             complement(<1..2887)
                     /gene="brxC"
                     /locus_tag="U8116_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HEN8435571.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFY"
     gene            complement(2912..3487)
                     /locus_tag="U8116_002644"
     CDS             complement(2912..3487)
                     /locus_tag="U8116_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HEN8435572.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(3487..>3985)
                     /locus_tag="U8116_002645"
     CDS             complement(3487..>3985)
                     /locus_tag="U8116_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HEN8435573.1"
                     /translation="IKEKNEHENIFFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQL
                     NIDNQKLVNLISILMIDDLFNEFMTEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDI
                     SKWKDYTIKRLIAAYQTNLIETGLVRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLA
                     LGGIL"
BASE COUNT         1189 a          762 c          545 g         1489 t
ORIGIN      
        1 tataaaaaaa gtcttcaaca tctgacgttt gaacaattac ttcaagatat ttttttattt
       61 gctcatattc ataagcacta gggtaggtat aagtgttttg taaatcaaaa taagagcgta
      121 aattgtctaa ttgacctttt aaatatttct tgaaattttc cattgtatgt tcattgttat
      181 tttcatcaac aatattacga ctttgaaaca gttctctacc gatttcgtca accaattttt
      241 ttgcttttgg cgaaatcacc gcgcttggtt caatttttat cttttcactt tctcgtcgtt
      301 tttgaataat cattacaata ctatttggat catccatccg tgaaatttgt acattgttat
      361 aaagtaaatc aattttacca gacatgaaaa gtttagcgat gcaccattcc acatcaagtt
      421 ctgtgaatcc atacggttgt tttgtttcaa aacgatcacg aattgttttc atcgtaatcg
      481 ttaggtgatt tctcttttga tcattgataa aattgaaaat ttcctgaact gcttgctcat
      541 ttgatggatc ttcaataatt tcatttttgc tttctttcac taaatataag atatcttctt
      601 tttctttagg cgcatcaatt tggtttaatt tatggtatac ttcttcagcc attttctgaa
      661 gtgctgattg gaattttgct ttaaagtcgg agcctgtcag ttgaatttct gcaccgttat
      721 agaaaaattt tgcagatttc aacagttcat ttaattgatc gttgatactt gcacttagtt
      781 cctgtaattc accagatttt tccgaacgaa tcgcgtcaaa gttctttact acactcgaat
      841 aagtcgttgt taaataaaga ttcattctag catacattgc tatatcatct ttaaattctt
      901 tatctgatgg caattctacg aataataagt tacgggaact cttaagcgca agttctgcct
      961 cttgacctgc taaatcagag tatggtgaaa taaaattaac tgtcatttca gcatttccag
     1021 cttgcttgta tggacgttga tctaacactt gtgcataagt aaacgtatac cggtttttaa
     1081 atttatttcc acctgacatg cctaattttt cataggcaaa actatttcgg tctagaacgc
     1141 cgtcaaaaat tcgtttagat agttctacga tcaaatcact atctgtaata ttttgatttt
     1201 taatctcacg agtaatttcc tgttcttcat cagtttggaa aatataacta tctaagttct
     1261 tccgcacaaa gttttcctta attaaaaggt ccaatgcttc ttctactcgt tttttagttt
     1321 ccaggcggtc ttcgttgata tttcttatca tcaagctcgt aatattttca acatttggtt
     1381 tgatggcttg atcaacatat ttaatcatga ataaaattcg taagacattt acgttaaagt
     1441 tatctatatc agttggtctt tgaatatgag aattatttgt agcttgtgta atcactcggc
     1501 taattgctgt atcaatccaa cgttctaacg agtaataaaa ctgatctaaa gaaaccaaac
     1561 tggttgtatc attttcctta tttcgttcag cggcttcttt aaacattgct agtaatgaac
     1621 gttcaccaga cgataagttt gatccttgat agctgtgatt acgaatctcg gttagaattt
     1681 gccccaataa attgaattga tagggaataa atggataaac ttctgcgtaa ttttcagcat
     1741 cttggaagag ctttaattca ggactatcaa caaagagtaa tttatttttc aattctgttt
     1801 gaacagtttc aaattgtaag gctaaagaat cattggcaaa ctcttttttc ttcaaaatac
     1861 gttctttaat tacttcatcg gcatttgctg aagataaact cacagttgtg gcaaaacgat
     1921 ccttgatctt agaaaaatca ttatcagaat tgttatcaaa gtttgttacg tgatcaatcg
     1981 cttcctgact tgtcactaat acccaagcct taccttcaca aattcgaccc aaatcttcca
     2041 caacggtttg taactgcaaa gtcaactcac gattaccgcc gatatattgt ccgacctcat
     2101 cgactagaaa ggcaatatga tggtctgctt ctttggaatc aagatagctt ttaactagat
     2161 ttgcaaagtc atcaatgctt attaaataat ttcctgatgc tttttctaaa aaattatcaa
     2221 tagcggcttc actcataaaa tcgattttaa gtgccgcttc tttgatatca tcttggatcc
     2281 aatcaaactc atggcgctct tcttgccatt ttgccccatt aatctcttca aaagcagctt
     2341 tgaatgcatc aaacttttct tctttaatta accgacgttc aaaatctgct agatgtggaa
     2401 tcgtgcttaa tccttgcatt tcgttaaata cctttaaaaa aacattcacg attccgtttt
     2461 tgtctttttt accacctgca tcactttttg aatcaatatt aaacagaata acatctgtat
     2521 ttatttgtga gcaccgttcc atcgttgcca aagtcagtgg atctttgatt ttcccatctt
     2581 ctttgaagta atcaatggct ttcttgtcat ttatttctcg atttgcgagc aagtaagaaa
     2641 taatttttaa gaaatgagat ttacctgaac caaagaaccc ctgaatccaa acacccattt
     2701 tatctgttgg tgtattgata ctccgttggt aggcatcaaa aattgtttta aagtgttttt
     2761 gtagttcttt cgtaaccacg tattcttcta attcttgttg aacattttca tcctttgctt
     2821 gaccgacctt aataactcct tgaatatttc ggtcaattgg ttttgcaaaa atatctttaa
     2881 ttagcattta atctcctacc ttccatccca actaaattct aaatgctcga taataattat
     2941 catctttatt ggtattaaga attgtcaaac ttaattcatc atattcccct ggatacatca
     3001 tcacaactgg tacgcgatca aatacctgat gcattttatt taaaatagag tgcgctctaa
     3061 ttaatggata taattcgcca attcctgtca ggaacacaac tgatttttct ggtgtatggg
     3121 catccacata ttcaaaaaat acattttctt cctcaggtaa atccattttc aataaacgac
     3181 caatgctttc cactaatttt tgtaaaccac gtcgttcttc catcttaatc gctttttcag
     3241 tgaaaccgcg tttttctaga tactctaaca taatcacata gagattataa actttcactt
     3301 cataattatc accaacagat gttccagata tatcattaat ataatctctt acaattaact
     3361 cgtctttcgg atcgtagtca aagatataat agcctacttc attacctaaa ccacgattca
     3421 ataaaaaatc ttctttttga attacttcat tcaacttttc caaatgctct tgaatcgata
     3481 atgacattat aaaattcctc ccagtgctag tacaatatct ttacgattct tgtcaactaa
     3541 ccattgaatc aatcgtttat ctaataatat cggagtcatt cgtccatatt ctttttcccc
     3601 tctgactaat cctgtttcaa ttagattcgt ttgataagcg gcaataagcc gtttaatcgt
     3661 ataatctttc cattttgaaa tatcttctga acttctccgc ttctctgtaa aaaaagaacg
     3721 gtaatcaact ggtgttaatt gcaaatttcc tttacgtaga ttttcagcat atacctcagt
     3781 cataaattca ttaaaaagat catcaatcat aaggatggaa atcaaattca ctaacttttg
     3841 attatctata tttaactgat tgaaaagttg caatccagct tcatccagtt gactaactct
     3901 ccttttcacg ttctgataga tttctttttg acggttactg ctcactgcaa aaaaaatatt
     3961 ttcatgttca tttttctctt taatt
//
LOCUS       DASNNU010000147         3923 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.152, whole genome shotgun sequence.
ACCESSION   DASNNU010000147 DASNNU010000000
VERSION     DASNNU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.152"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(573..1130)
                     /locus_tag="U8116_002646"
     CDS             complement(573..1130)
                     /locus_tag="U8116_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435574.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="U8116_002647"
     CDS             complement(1309..1920)
                     /locus_tag="U8116_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435575.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="U8116_002648"
     CDS             complement(2033..2290)
                     /locus_tag="U8116_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435576.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="U8116_002649"
     CDS             complement(2887..3165)
                     /locus_tag="U8116_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435577.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="U8116_002650"
     CDS             complement(3199..3450)
                     /locus_tag="U8116_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435578.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DASNNU010000148         3793 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.153, whole genome shotgun sequence.
ACCESSION   DASNNU010000148 DASNNU010000000
VERSION     DASNNU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.153"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..709)
                     /locus_tag="U8116_002651"
     CDS             complement(<1..709)
                     /locus_tag="U8116_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435579.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVIINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFILLF
                     VERFNQAFHTDYATNIFEDFENSYSYSVISSTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNGFSLHQV"
     gene            835..1500
                     /locus_tag="U8116_002652"
     CDS             835..1500
                     /locus_tag="U8116_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8435580.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(1897..>3793)
                     /gene="brxL"
                     /locus_tag="U8116_002653"
     CDS             complement(1897..>3793)
                     /gene="brxL"
                     /locus_tag="U8116_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HEN8435581.1"
                     /translation="SLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVI
                     DKVTVHLNEKKDCYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDEND
                     RWNSPFIAKKVDPIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWL
                     HLARLIPLIENNFNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTM
                     GLVGMWDCVAFDEVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQ
                     SVDVILKTSNLFEPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFS
                     EVMRELRKVSFGDSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEI
                     LRFALEMRRRVKEQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGK
                     PGHLYTVSYGKTGQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYG
                     SVSQSISLTTKDFVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITI
                     GGSMIKVDNLANCLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAV
                     FKALGVE"
BASE COUNT         1267 a          763 c          559 g         1204 t
ORIGIN      
        1 atacttgatg taatgaaaag ccattattca acaataaact gatttcttta ttcatcaata
       61 agaaaaagga tgataacaaa aggaataaaa gaatcaatcc aatagttaaa tacactcgat
      121 aattatatac gtctgtactt gaaataaccg aatacgaata gctattttca aagtcttcaa
      181 aaatattagt ggcataatca gtgtgaaatg cttgattgaa tcgttccaca aatagtaaaa
      241 tgaaccgttc ataggcttct ttttctctcg tcatcaagaa aaattcacct tcaaaatctt
      301 ttattttgat cgattcttca atggacgtta cgcgaaagga ggtattagaa tagggtacct
      361 tctcgtgaga agatttgaca tccccttgat aatacaatgt catttcaagc tttggctgta
      421 tcttgaagtt ccaagatgta tctacttcat tggattcatt tactgttttt ttcatataag
      481 aaagctcagt ttctgatgag acattttcaa ataccatgat tagctgctca tattcttctt
      541 gcccaataaa atgttgtggc aatcgaatcg gagtataagt atctgtttga tggaaaaatt
      601 catttgtccg atagagtacc tgtttattta taataaccaa ggcaaaacca ctcatgatac
      661 cggtcactat gattgtgagt aaaactgtga actttttttt gattatcacc actctccctt
      721 acttaaatga ttctttgata aaatcatacc gtttttcatc aaaaaaacgt atctatcaaa
      781 tgtataggtc atgtagaata aatctatata ctgtagaatc gaaaggagaa gaagatgaac
      841 atcggagaaa aaatacgaac ttaccgcaga aattgtaagc ttacgcaaaa agaattaggc
      901 gaaaaaatcg gtgtttccga taaaacaatc tctagttggg aaaattctcg tacaatgcca
      961 gaccttgaaa tgctgtcact tttacatcag actttaggtc tgccgattga ttttccttct
     1021 attgaatcag ctaccgaatc aatagaacca acattgccgg atgaacctct tgttatcaag
     1081 aaatcttatt catcaaaaca acccttctca ctcaaaaaga cactatcttt ctttctgctc
     1141 gtcttttgtt tggtctatct ttttcttatc aattatcaat ataaaaatga aacggttgat
     1201 cgattcaatc cttttttgaa accacaaatc ggctatacca cactccctgt aggatacgtg
     1261 gaaaattcca tcgaacattg ggtatgtaat gatatctttg ttcatggtac atggctgtct
     1321 tttcacgctg gagaggtaga aaaaggtaag aatcatgctg taatcaaaca caaaggaaaa
     1381 cacgtcatga acgttcgcct gctcacttgg gaagccattc cctcagatta ccaagaaatc
     1441 ctcatccaag actatgacga tgccttaaaa agaagccctc aggagccaga taggcattaa
     1501 gtaggagatg aacgtagaga tagaaaaaat caatgcacat gatcttatca aaaaaaaaca
     1561 ggggtacaaa ttaattgaaa aagctttgag atgaaagttc ctaaataaaa aagttcagaa
     1621 tgataggtaa aaaaatgact aactattcta gtattaggct atttgtatta ttcttctgtt
     1681 atttttatat caaaaaatcc agaaataact aaatataggt atttctggct tatagtataa
     1741 tatttacgcc acagccccca atgattcttt ttggaatcat gatcaaggac ggacatacac
     1801 aaaaatattt tgcccattcg atccgaatag attaacgact aatctagtaa gtaatataga
     1861 ttggtacgta gtcaaaccag caaatggaaa agttgtctat tccaccccaa gggctttaaa
     1921 gacagcatct tccggcgttt gataaaaaat caaattaaag gtacccatta attctggggg
     1981 tacagttcca atatcagcag cgcttatcat aggtacaagt acttttttcg ctccggcatc
     2041 ttttgctact tgtaaacagt tagctaaatt atctacctta atcatagaac caccaattgt
     2101 aatatcccct aaaataacta aacttggtaa tacaggttta tttaaggaaa ctgagcataa
     2161 agctattata gttgataacg ataatgtttt tgtcatacca atgccattca tatcttgaac
     2221 atgaacgaca aaatcttttg tcgtcaaact aatagactga ctaacacttc cataattagc
     2281 tcttaaatat cggtatgccg aatccatcgc ttctcttgct ccagcatctg aattgatccc
     2341 agtacgttca aactttccat ttcccgaagt catttgcgtt tccaatttat aggtacctaa
     2401 ttgcccagtt ttgccataag ataccgtgta caaatgccct ggcttcccta acccttctgg
     2461 aatcaaagtt cctccacctt gttcaggaac agaaacatac tcttctcgct cttcttctaa
     2521 atcaatgtat gaaaaattga catcataaaa ttccattcca ccaattttct ttaattgttc
     2581 ttttacacga cgacgcattt caagagcaaa tcttagtatt tcttcgacat cacttttttc
     2641 aaatctacca tcaggataca acagtttcat catcccagat acaatccgac gaactgcaat
     2701 cgtatcccgc tgatttaatt gagaaccaag tttgaaatat ttatcaaaac tatcgccaaa
     2761 actaactttt cgaagctcac gcatgacttc agaaaaataa tctgtaatga agccatagtc
     2821 atttgtaaaa aaatctggtc gatatttagg aatttcccaa cctggtaaat aacaatgcat
     2881 ccgatccaaa aaggctgaat ccgtccccat aacctccgga aatggctcaa ataaattcga
     2941 agttttcaat attacatcaa cgctttgatt aatatttcct acaaaagcca ttgatgctga
     3001 agcattcttt tcttctttgc ctctagcaaa agatcctgaa gccatgtaat ctttcatgat
     3061 ttgaacgcca tcattatcct taaatttaat cccagcaact tcgtcaaaag caacacagtc
     3121 ccacatacct acaagtccca ttgtacgact agccatatta tagaaaaggt ttgctactgt
     3181 tgtttgtcca cctgaaacaa ggattgagtt tggtgaaatt tctttatata catgcgattt
     3241 acctgtacca cgtggtccta gttcacagag attaaaatta ttttcaatta atggaataag
     3301 tcttgctaaa tgaagccatt ttaccctttt attaaattgt gtaggttcca taccaatgga
     3361 ccgtagaagt aagtcaatcc attcatttgt agtaaactcc ttgcgagcca tcttgtaagc
     3421 ctcaatgtct aaaccaggca tttggattgg atcaaccttt tttgcaataa aagggctatt
     3481 ccaacgatca ttctcatcat accaatattc catcgaaaca atacaccaga tccccccacc
     3541 taataatcga tcaaattttt tcggatactc ttcactaatt tgaacatttt ttaatcccag
     3601 gttagagaat tctgcttcat aacaatcttt cttttcattt aaatgtacag ttactttatc
     3661 aataactgta taaccaccca attcacgaat ttttgctttt actttttctg cttcatcagg
     3721 acgcacatag tttcgagcaa gaatattttt aacattttcc acaccttttt caaccaatgt
     3781 ttcatccaat gac
//
LOCUS       DASNNU010000149         3777 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.154, whole genome shotgun sequence.
ACCESSION   DASNNU010000149 DASNNU010000000
VERSION     DASNNU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.154"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2232)
                     /locus_tag="U8116_002654"
     CDS             complement(<1..2232)
                     /locus_tag="U8116_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435582.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="U8116_002655"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="U8116_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1099 a          806 c          580 g         1292 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa tttctataaa
     3541 aaaacaatca ttcttaaaaa gaatatataa aaataacaat aacctttgtg atttttcttg
     3601 tttgaagaat gttagaagaa aaatctctaa atacttataa ttattttata agcaaaaaaa
     3661 tgctatcggt agtatcaccg atagctgagg aaagtcacag tagggactat ttgattaaaa
     3721 aatatgttta ttcaatccat ctatttattt ttgccttatt aacatattgt aataatg
//
LOCUS       DASNNU010000150         3616 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.155, whole genome shotgun sequence.
ACCESSION   DASNNU010000150 DASNNU010000000
VERSION     DASNNU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.155"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(235..912)
                     /gene="nth"
                     /locus_tag="U8116_002656"
     CDS             complement(235..912)
                     /gene="nth"
                     /locus_tag="U8116_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEN8435583.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(919..1617)
                     /locus_tag="U8116_002657"
     CDS             complement(919..1617)
                     /locus_tag="U8116_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN8435584.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1885..3069
                     /locus_tag="U8116_002658"
     CDS             1885..3069
                     /locus_tag="U8116_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEN8435585.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3075..3518)
                     /locus_tag="U8116_002659"
     CDS             complement(3075..3518)
                     /locus_tag="U8116_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN8435586.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          933 a          722 c          608 g         1353 t
ORIGIN      
        1 tttggaatac tggacttatt ttataggtat attggaaggc attattttct acatcgggta
       61 ttttcccaat aaaaataaca acttttattt ttatcgcaat aaattgtaat aagttaccat
      121 cctagctact gagtatgtac atttactttt tcatgcaaaa aagataaacg aaacagcttt
      181 tctccataat aacaagataa ttcatacatt tatttgcaat taaagaaacc acgtctacag
      241 gcgtggtttc ttttttagcg ccttttcttt tagacgcatc cgatttttcc catcttgaca
      301 tattgatagc aaaggacata cctcgcactt gggatttctt gccgtgcaat gatatctacc
      361 aaaaaaaatc agtgtatgat gtgtctttac ccataattct tgtggtactt tccgcatcag
      421 tgtttcttct acttccatga ccgtagcatc tagtttgcag atcctcaggc gttttgagac
      481 acgttccacg tgggtatcta ctgcgattgc tggtatcccg aatgcatccc ccaatacgac
      541 atttgctgtc tttctgccta caccaggaag agacatcaat tcttctcttg acgttggcac
      601 ttgcccatcg aaacgttcaa tcagctgctg tgcacacgct ttgatgtttt ttgctttatt
      661 tcgatacaaa ccaatcgttt ttattttaag aatgatttca tcgattgaag cttctgctaa
      721 cgcatcgggt gtcggaaaag aggcaaacaa atctggcgtt gctttattca ctgatacatc
      781 tgtcgcctgg gcacttaaga tcactgcaat caataactca aatggattat tgtgcttgag
      841 ttctccatgt gcttcgggaa acattccata catcgtttct agagcttcca ttgttttttg
      901 tttgcttaac atattccgct actcctccaa ccaatttttc agtgagactt tcggcaattc
      961 ttcttgttga gctgactgtt ctacttctct ttgaagaagc tgctgttttc ttcgtttttg
     1021 gtcttcttcc acttgttgtt ttgtacgtag attttttctt tcccacgata gcaaaatgcg
     1081 gtcaacgtaa ttaaaactat aagcctgatt taatacggct tcccgcagtg ctagcttcaa
     1141 aatttccggc tgataatggt cttcttctag ccattgtccg atccgctgga actcgatcga
     1201 agaaagcggg cgtccaaatt cttgttcaaa taattgatac actgactgga tttctttttc
     1261 ttgtgttttt tcttcgtatt ttttttcttg tgtttttaaa tactcaccta atagatcata
     1321 gatggggtaa agattatatc gatctgcttt tttaccgttg acaatcgtcg tttctatctt
     1381 gataaatcca tttgttacga gacgatccag tatttgaaaa atctgatctt gtttgattcc
     1441 catatccata gcaatcatct gcaaatcagg gaaagtatct ccttcaagct gtgccatatg
     1501 caactgtaga acaaataaaa attcttcaga ttgtagaccg atcctatgat aatttttaag
     1561 taaaagatta gatatgactg tttgtccagc gcttaagtaa ttttctaaat tcaacatgat
     1621 ttctcctccg ctcaatcaca gtatacgtaa aaaaaacatg ggaagcaaga acaggtttct
     1681 tttctattta cttcactgat cggtcagatt tacaatgtcg tctacttgat ttttgtttgt
     1741 tcatgttttt gatgcgtatc ttcacttttc ggaataaata aattaagtag atttcgctgt
     1801 tccttagaat gttgacaagg aaacaaaatt actatacact atttttgttt ccaagttaac
     1861 attattgcat aaaggagttt ttacatgtac agtttcacaa ataaacattc taaagttttt
     1921 ggatacgtct ctgtcttatt cactggatta ggatttacta tcgtcagtcc tgtacttccg
     1981 tttttaacgc tgccttattc tcactccata catcagcaag ctttctatat cactttgctc
     2041 atgtctgttt atgctctagc tgccttctta tctgccccca ttctcggtag cttaagtgat
     2101 cattttgggc gtcatccgat ccttattatc agtttattag gatcaagtat cggatatttg
     2161 ctttttggtt taggaaattc gatatggatg ttgttccttg gtcgtatcat tgaaggattg
     2221 acggcaggag aaatctccac cttatatgct tactttgccg acatcacgga acctaatgaa
     2281 agaaccaaag tttttggatg gatgggtgct ttagtcggta taggaacaac gctgggtcct
     2341 attatagggg gtctattagc tgagttgggg aattctgttc ctatatttat tggagcactc
     2401 tttactttct taaatgccgt ttatggctac acatttatgc tagaaagcct tccaataaaa
     2461 aaacgttcag tagatttaag tttctcccat gtaagacctt ttcaccaatt acaacaatta
     2521 ttcaagattt catccgttat tcctttattg acagcaggtt ttgctgtttg gttggcagct
     2581 ggctctcttc aatctatctt ttctcaattt tcgattgacg cctttcagtg gaaggctggg
     2641 ttggttggtt taagcttctc tttgattgga atcttggata ctgtctcgca attgttcatt
     2701 atgcctcgat tgctcaaaaa attttcagaa cagcagatca caagaatagg gatgttcagt
     2761 gaaatcctcg cctatctatt catcacatta tctggaattc ttctactgcc gattttgttt
     2821 ttatttggga ttatctgtta tggtttcggt gattctattt ttactcctgt attcaatggc
     2881 caactatcca attctgtctc agataaccag caaggtcttg taatgggcgg tacccaaagt
     2941 atacaatctc tatcgcgcgt catcggcccg ctaatcgcag gacagcttta tgctgcagca
     3001 ccatttcttc cgacggtatt cggatttttg cttatgattt ttgcctatta tcactacaat
     3061 aaaaaatagt agcttcattt gctacttcgt ataaattctc tgtatcgacc gataaaacga
     3121 aatactgtgt caaattcttc ttttgacata ttttcaaata cagtagcatc tcgctgtacc
     3181 catttttgat ggagttgctc gtgtctattc cttatttttt gtccgattgg cgtcaattga
     3241 aaatagattt cttttttatt ctcttcgctt tgataagtgt ttatgatccc tctagctatc
     3301 agtttttttg ttagcttact gatggctcct ctagtcatgt aactagcatt cgctaatttt
     3361 gtcacgttag gatcagggat tttcccgata tgttcaatca cttctagttc atttagagta
     3421 aatcctgcaa attctttttc catttctggt ttattacgat gaataatctc attagctaaa
     3481 tccagatatt cttctattag cagttgtctt ttatccattt ctacaccttc tttttctttc
     3541 attatattgt ttttttgttt cctattcaac aaagataaaa atattttgta ggaaaaccca
     3601 atctgaaacc aagaaa
//
LOCUS       DASNNU010000151         3607 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.156, whole genome shotgun sequence.
ACCESSION   DASNNU010000151 DASNNU010000000
VERSION     DASNNU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.156"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..144
                     /locus_tag="U8116_002660"
     CDS             <1..144
                     /locus_tag="U8116_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435587.1"
                     /translation="TLDEKRQKELQQLKQKYGKSESKIMCIALDLLIAQEKAGFDIPA
                     LKK"
     gene            485..748
                     /locus_tag="U8116_002661"
     CDS             485..748
                     /locus_tag="U8116_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HEN8435588.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            742..1092
                     /locus_tag="U8116_002662"
     CDS             742..1092
                     /locus_tag="U8116_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN8435589.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1116..1730)
                     /locus_tag="U8116_002663"
     CDS             complement(1116..1730)
                     /locus_tag="U8116_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN8435590.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            2044..2466
                     /locus_tag="U8116_002664"
     CDS             2044..2466
                     /locus_tag="U8116_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435591.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2476..2679
                     /locus_tag="U8116_002665"
     CDS             2476..2679
                     /locus_tag="U8116_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435592.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            2890..3474
                     /locus_tag="U8116_002666"
     CDS             2890..3474
                     /locus_tag="U8116_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN8435593.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         1255 a          522 c          642 g         1188 t
ORIGIN      
        1 acattagatg aaaagagaca aaaagaattg cagcaactaa agcagaagta cggaaaaagt
       61 gaatctaaga ttatgtgtat tgcgttagat ctattgatcg cgcaagaaaa agcaggattt
      121 gatataccag cactaaaaaa gtgacgtcac ttttcatttg aagaaacaaa agtgatagca
      181 cttaattgtt cctactattt agaatatttt ctatgtcctt tttattccat tttgtatcaa
      241 taactatgaa atctttaaag tatccagaag agattatttt tagatcaatt tttttcttac
      301 ctgattctaa agattttgtt ttcttaaaca tattatgatt tcctttcgac tattttcata
      361 taaatgttaa catagtttat ggattcatgg aatgttaatc taatttaata gaaaaggata
      421 attaagtata tacgtttttt cttatgtatg ttatactatg tatatacaga aagaggtgtc
      481 tactatggaa atgttaccta taaaaaaatg gggaaatagt aatggcctta gattgccaaa
      541 gtatattatg gaatatcttg gaattcatac agaagacaaa gtgaagataa tacaagaaga
      601 agtaaacggt cagaagcggc taattatcga agcggctaat atagaaaatg aattaacaat
      661 tgaacagtta tttgaaaatt acactgatga aagaaatcat gtgacgattc aaaatttagg
      721 tgaagcagta ggtaatgaaa aatggtgaga aaacctagac aaggcgatat tctattattg
      781 aatacagcac ctcgatctgg acacgaacag acaggtaaaa gaccttatat tgttttaagt
      841 catgatatta ttgcggatta tagtaacgtt gttacagttg ctccaatttc tagtacaact
      901 agaaactatc cgctatatgt aaatataaac tcagaatatg aaatgaagac aacaggaaaa
      961 gtattattag atcaactaac gacaattgat tatgaagcca gagagtgtat ttttttggaa
     1021 aaagcgcatg atagtttagt tgaggagtta ttgatgaaag ttagaacagt ttttcaaaaa
     1081 gtaagcaaat aaaatagaaa cagcagcatg atcaactatt cgtgattgac catgctgtct
     1141 ttttttattc tatatacggt ttgtctcgta acattgtagt cttttgcaat cttagcgatc
     1201 gcaacacctt tttttagatc ctcaacgatg tttttataaa caagacgacg ttggggatct
     1261 tttgcattgg cactgtatag cttaggacgt cctttataaa caccgttcgc ttttgcaatc
     1321 ttaatgcctt gtgcctgacg tcgtttagat tcggtacgtt cttgttcagc gatcatggcc
     1381 aaaatttgaa tgatcaaatc tttaatgaat ttatccagga gaggattgcc aattgcttcg
     1441 gccatgatcg gtagacttgt gatgatcagc tgcacatttt tcttcttcaa gtaattgact
     1501 gaagcgatga tctcgtcgta atttcttcca agtcgatcaa tagcttccac aataaaaata
     1561 tcttgatctc ggacgaaatc cagtgcttct tgaaaaacaa gtcgatgagt aatcgttgcg
     1621 ccagattgtt tctcaatgaa aattctctct gcaccgaatt ttttcatttc ttcaatctgc
     1681 cgttgttcat tttgatcgat ggatgagaca cgtatgtatg caactctcaa aatttttacc
     1741 tactttctgc aactatttaa aaaacaccct aatcatacac gagaaaacca gatttttcaa
     1801 tgtcgcggtc gtgtctagtt agggtatacc ttatatagac aaaaactaat gatggaggaa
     1861 atagtatgtg tcacttttct ttttaaatgg caatattttt tgtaaataaa tgtcaatcaa
     1921 gaaaatttaa acgattatga cattaaaaat atattttatg aattttctag tatataactt
     1981 gatttatctg ttaagattta ttaagaaaac gttttcttat ttttgatttc gaggtgatag
     2041 acaatgaaaa ataagtttat tttatcttta ggtattttaa ctattggaat tagtgtaact
     2101 ggaactacaa ttcctacatt tgctgatagt acaaatgaac caactactgt agtagataat
     2161 aaccaagaat taactccttt agataggaac ggaatagcca ttggtaaccc aactaacgac
     2221 ttaactgtta ctgaaaatta tttaatagtt tctccattac ttgctcgagc agcttctaaa
     2281 acgatacgag tgacaggaac gatgattttt gataaagttg gttggccttc tagacctcct
     2341 caatcacgga attggaaaga aaaaagaaat ggttattggt actatggaaa attgtattta
     2401 caaagttata aatcagctgg aaacaattgg attgcgactt attcaggaac cttaactaga
     2461 aagtagggat tttttatgcc agaatttgtg aacacaccaa atttttatca agatagcgat
     2521 attctaagta aaattaattt accgaaagaa gaatcagctt tacaagttca gcgaacttta
     2581 caggagacac aaaaaattca aaaatcaatt tctaatctag aagattctga agaaaaaaga
     2641 aaaaaaagag accaaaaaac atatcaaaaa gaaaaataat gattttaaaa aaagagtgtc
     2701 tagtcgacat tctttttttc taattgaaag tgttcataaa atcacccatt tctggacact
     2761 ttcttttttt gctttatttt tcaaaaaaac aattcatttc atgttcataa acccataaca
     2821 atatctatgt tcagttaaac tatatggata aagttatgtt acaataaaat taatgaagga
     2881 gtgatttttt tgaaaatagg atacgcccgg gtatctactg gtttacaaaa tttggattta
     2941 caagaagaca gcctaaataa atttggttgt gaaaaaattt ttactgatca tatgagtggg
     3001 gcaaaaagta atcgaccagg attagaaatg gccattgatt ttgttcgctc tggcgataca
     3061 cttgtcgttt ggcgcttaga tcgtttaggt agaaatatgg aagatttgat ttcaatcgtc
     3121 aatcgactga atgagcgtgg tgtgagtttt catagtattc aagaaaatat tacgatggac
     3181 aaatcaagtt ctacaggaca gctcatgttt catttgtttg cggcatttgc agaatttgag
     3241 cgtaatttga tcctggaacg atccgcagca ggacgagcag ctgctcgtgc aagaggacgg
     3301 tttggtggtc gtcctgaaaa attgtctgaa caagatttgg aattactgaa gactttagtg
     3361 gatagcggta cacctattaa gactattgcg gaacggtgga acgtctcaag aaccaccatt
     3421 tatcgttatt tggcaaaaat ggaggagaaa aatggaaaga caaattctca ttagtttttg
     3481 tcatttaatg ctgaaaattt ttgccattta tctagaaaag tgacaatagt atgagaattt
     3541 tttgtaagtg tctactgata acttattctc aaatgttgta aggattttat tcatttgtta
     3601 atattaa
//
LOCUS       DASNNU010000152         3575 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.157, whole genome shotgun sequence.
ACCESSION   DASNNU010000152 DASNNU010000000
VERSION     DASNNU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.157"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..1194
                     /locus_tag="U8116_002667"
     CDS             4..1194
                     /locus_tag="U8116_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEN8435594.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            1298..2053
                     /locus_tag="U8116_002668"
     CDS             1298..2053
                     /locus_tag="U8116_002668"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEN8435595.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            2169..3467
                     /gene="eno"
                     /locus_tag="U8116_002669"
     CDS             2169..3467
                     /gene="eno"
                     /locus_tag="U8116_002669"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEN8435596.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         1245 a          674 c          752 g          904 t
ORIGIN      
        1 aaaatggcta agaaaacagt gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt
       61 gttgacttca acgttccttt gaaagacggc gtgatcactg acgacactcg tatcaaagca
      121 gcattgccaa caatcaacta cgttctagaa caaggcggaa aagcaatcct tttctctcac
      181 cttggacgtg taaaaacaga agaagataaa gaaggaaaat ctttagctcc agttgcaaaa
      241 cgtctaggcg aattattagg taaagaagta acattcgtac ctgaaactcg cggagaacaa
      301 ttagaagaag caatccgcaa catgaatgac ggcgacgttg tcgtattcga aaatacacga
      361 tttgaagata ttgacggtaa aaaagaaagc aaaaatgata cagaactagg taaatactgg
      421 gcttcattag gcgatgtatt tgttaacgat gctttcggta cagctcaccg tgcacatgct
      481 tctaacgtag gtatcgcttc aactggtatc ccaacagttg ctggattctt gatggaaaaa
      541 gaaatcaagt tcatcggtga agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt
      601 ggtggcgcaa aagtttctga taaaatcggc gtaatcgaaa acttgattcc taaagcagat
      661 aaaatcttga tcggtggcgg aatgacttac acattctacg aagcaaaagg aatcaagatc
      721 ggtaactctc tagtagaagc tgataaagtg gaactagcaa aagaattaat cgaaaaagca
      781 ggcgacaaac ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc aaatgatgtt
      841 gaaacacaag taatcgaagg agacattcct gatggtttga tggctttaga catcggccca
      901 gcatcagtaa aattatttgc tgatacatta caaggtgcaa aaacagttgt ttggaacgga
      961 cctatgggtg tatttgaaat gagtaacttt gctaaaggta caatcggtgt ttgtgaagca
     1021 atcgctaact tagaaggcgc aacaacaatc atcggtggcg gagactcagc tgcagcagca
     1081 gaacaattag gatttgctga caaattcaca cacatctcaa caggtggtgg cgcaagcttg
     1141 gaattgcttg aaggtaaaga attaccagga ctagcagcaa tcaacgataa ataaacgacc
     1201 aagtttgctc aattctgacg agtaatcgat caaaaggtga agggagtttt catgactccc
     1261 tagccatttt tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg
     1321 taactggaaa atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa
     1381 caaaatccct tcaaatgaca aagttgattc agtcatcgga tctcctgcat tattcttaca
     1441 agaattaatg acatcagctg aaggtacaga actaaaaatc tcagcacaaa actgctactg
     1501 ggaaaactca ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg atctaggtgt
     1561 tcattatgtg atcatcggac attcagaacg tcgtgaatat ttccatgaaa cagacgaaga
     1621 catcaataaa aaagcaaaag ctatctttgc aaatggtatg acaccaatcc tatgctgcgg
     1681 cgaatcttta gaaacctatg aagcaggcaa aactgctgaa tggatcgaag gacaaattac
     1741 agctggttta gctgatcttt cagctgaaca agtgtcaaac atggttattg cttatgaacc
     1801 aatctgggca atcggtactg gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg
     1861 agttgtacgt caaacagttg aaaaacttta tggaaaagaa gtatcagaag cagttcgtat
     1921 ccaatacggc ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt
     1981 tgacggtgct ttagtcggtg gcgcaagctt gcaagcagat tcatttttgt cattgttaga
     2041 cgctgtaaaa taattgttga aaatttgcga aaaatgcaag aaatgtttgc actaatgagc
     2101 aaaattaact aaaatcgtat atgagggaaa tcccttagga aaaaacaaac tcaaaggaga
     2161 gacaaaacat gtcaattatt actgatgttt acgctcgcga gatcttagac tcacgtggta
     2221 acccaacaat cgaagtagaa gtatacacag aaagcggagc ttttggccgc ggaatggttc
     2281 cttcaggtgc ttcaactggt gaatacgaag cagttgaatt acgtgacgga gacaaagctc
     2341 gttatggcgg taaaggtgta actaaagctg tcgacaacgt aaacaacatc attgctgaag
     2401 caatcattgg ctacgatgtt cgtgaccaaa tggctatcga caaagctatg atcgctttag
     2461 acggaactcc taacaaaggt aaattaggtg cgaacgctat tttaggtgtt tctatcgctg
     2521 ttgcacgtgc agctgctgac tacctagaag tacctttata ccactaccta ggcggattca
     2581 acacaaaagt attgccaaca ccaatgatga acatcatcaa cggcggatca catgctgata
     2641 actcaatcga cttccaagaa ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag
     2701 ctctacgtat gggtgctgaa gtattccacg cattagcttc aatcttgaaa gctcgcggat
     2761 tagcaacttc tgttggtgac gaaggtggat ttgctccaaa ccttggttca aacgaagaag
     2821 gttttgaagt aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt aaagacgttg
     2881 ttcttgctat ggatgctgct tcttcagaat tctacgacaa agaaaaaggt gtttacgtac
     2941 tagctgactc aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat
     3001 tagtttctaa atatccaatc atctcaatcg aagatggatt agacgaaaac gactgggacg
     3061 gattcaagaa attaactgac gtattaggcg acaaagttca attagttggt gacgacttgt
     3121 tcgtaacaaa cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct aactcaatcc
     3181 ttatcaaagt gaaccaaatc ggtacattaa cagaaacttt cgaagctatc gaaatggcta
     3241 aagaagctgg ctacacagca gttgtatctc accgttctgg tgaaacagaa gattcaacaa
     3301 tctctgatat cgctgttgca acaaatgctg gccaaatcaa aactggttct ctatcacgta
     3361 ctgaccgtat tgctaaatac aaccaattac tacgtatcga agaccaactt ggtgaagttg
     3421 cagaatacaa aggtttgaaa tctttctaca acttaaaaaa caaataattc ttttctaatg
     3481 aagatacatg atcgactatt gaaagatagt cgagtatgat cacttagtta agtaatcgaa
     3541 ctctctactt gctgaaatat cagcaggtag agagt
//
LOCUS       DASNNU010000153         3475 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.158, whole genome shotgun sequence.
ACCESSION   DASNNU010000153 DASNNU010000000
VERSION     DASNNU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.158"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            88..735
                     /locus_tag="U8116_002670"
     CDS             88..735
                     /locus_tag="U8116_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435597.1"
                     /translation="MKKIVLALSVFVGSVFLLSDKVSASQIENPHEINFSVQNIDTGE
                     YENENVEILTNKTYEVPSLRSSMITYGNSSEEKSYVSEFEIEFTPEDENDSNTIKPFA
                     SNDNETNSTGAVKVLARITYTKSGDNIKVTSASSRFTLTSSFASVSSRNLKVQQGFTN
                     ALEKTPTSNSLTTYNTGWGYVYNYGGAATAKTTVSGTVTMSGMASYRVSATASRL"
     gene            751..1392
                     /locus_tag="U8116_002671"
     CDS             751..1392
                     /locus_tag="U8116_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435598.1"
                     /translation="MKKILITLIGFVSLTILFGNTLQAKADSLASIPTVEAKITSAEG
                     VVGEGSTQTKVIEHKELAYLSKFSTVPALEEKKYETTIEVEYYFTPEESGSTSISPLS
                     TNTGSANNGIAKSSITLTYWISGNTIKVEKCHGSWSKASTSSIIVSDRYVTYRGPNAQ
                     QTAWKYPTSNTFSYNTGWGYASQNTAGNGRAYMSAYTSISGMGRLFLEAALNL"
     gene            1775..2410
                     /locus_tag="U8116_002672"
     CDS             1775..2410
                     /locus_tag="U8116_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN8435599.1"
                     /translation="MSRKCEQKLTIYGYSRVSTSSQDYKTQIEKLEQAGAEKIYSEKY
                     TGTKKDGRKELEELLSIVEKEDVVLVTKIDRLARSIVDLNSIISTFNKSGVTITFIDN
                     SLTFEPYKNDSMQTLMMNMLGSFAQFERDLIVTRTQEGKQWQRANKKDYKEGRPKRIL
                     NDRYKHALELMKTNSMKEVVKKTNLSLSTLKRIKKQAKEEGIYNNEEKKTV"
     gene            2388..3475
                     /locus_tag="U8116_002673"
                     /pseudo
     CDS             2388..3475
                     /locus_tag="U8116_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230870.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1325 a          467 c          643 g         1040 t
ORIGIN      
        1 tattatttat ttaccgcaaa gggaaaatga tgtataatgg gttaaggcta aattaaatta
       61 gtctaataaa attatttgga ggttatcatg aagaaaattg ttcttgcgtt atccgtattt
      121 gttggtagtg tatttttgtt gtcagataaa gtttcagcta gtcaaattga gaaccctcac
      181 gaaataaatt tttcagttca aaatattgat actggggaat atgaaaatga aaatgttgag
      241 atactgacta ataaaacgta tgaagtacct tctttaagat caagtatgat aacttatggt
      301 aattctagcg aagaaaaaag ctacgtttct gaatttgaga ttgagtttac tcctgaagat
      361 gaaaatgaca gcaatacgat caagccattt gctagtaatg ataatgaaac aaattcaacc
      421 ggagccgtga aagtacttgc aagaattact tatacaaaat ctggagataa cattaaagta
      481 actagcgctt cttccaggtt tacattgacc agctccttcg ccagtgtttc aagtagaaac
      541 ttgaaagttc aacaggggtt taccaatgcc ctagaaaaaa cgcctacttc taattcatta
      601 accacgtata atactggatg gggatatgtt tacaattatg gtggagcagc tactgcaaaa
      661 actacggtat caggaacagt aacaatgtct ggaatggcta gctatagagt gtccgcaaca
      721 gcctctagat tataaaaaag gagaactcaa atgaaaaaaa tacttataac tctaatcgga
      781 tttgtttcat tgacgatatt gtttggaaat actttacagg ctaaagccga ttctctagca
      841 tctattccta cagttgaggc aaagattact tccgctgaag gtgttgtcgg agaaggaagt
      901 acacaaacaa aagttatcga acataaggaa ttagcttatt taagtaaatt ttctacggtt
      961 cctgcattag aagaaaaaaa gtatgagact actatagaag tcgaatacta ctttacccct
     1021 gaggaatctg gtagtactag tatttctcca ttatctacta ataccggttc agctaataat
     1081 ggtattgcga aatcgagtat aacgttgacc tattggattt ctggtaatac tataaaggta
     1141 gaaaaatgtc atggatcttg gagcaaagct tctacctcat caatcatcgt ctccgatcgt
     1201 tatgtaacgt atagaggacc aaatgctcaa cagactgcat ggaaatatcc taccagcaat
     1261 accttctctt ataatactgg atggggatat gcttcccaaa atacagctgg aaatggacgt
     1321 gcatacatgt ctgcttatac cagtattagt ggtatgggaa gattattctt ggaagcggca
     1381 ctaaatctgt aattgcttga aaaatatttg attataataa taaatatccc tcgtcagatc
     1441 aaaattattg gcgggggaat gtgtattcct ggatacactt ataaattata aaaattgttg
     1501 agcaaattaa aaatctagta acagaatatc tatgatgaaa ttgcgaaaaa gagcgttcag
     1561 gaaatgactt atttctggac actcttttat ttagatcaat tgctatgaaa aattattcta
     1621 tatcttgtac tgaaagccag aacaaaatct atattctggt aagaatagaa ggcgatttat
     1681 tttaaaaaaa ttcatttaag ggtcagaaac ttaaaagttt ttgacccttt cacactggct
     1741 tgatatactt gactttgaca ggtgtcagaa acttatgtcc agaaagtgtg agcaaaaatt
     1801 gacaatttac ggttattcaa gagtcagtac aagcagccaa gattacaaaa cacaaattga
     1861 gaaattagaa caagcgggtg ctgaaaagat ttacagtgaa aaatatacag gaaccaaaaa
     1921 agatggaaga aaagaattag aagagctact ttcaatagta gaaaaagagg atgtagtttt
     1981 agtaacgaaa attgatcggt tagcaagaag tattgtagat ttaaattcta tcatttctac
     2041 ttttaacaaa tcaggagtga caataacctt catagataat tcgttgactt tcgaacctta
     2101 taaaaatgat tcgatgcaaa ctctgatgat gaacatgtta ggaagctttg cgcaatttga
     2161 gcgagatctg attgttacac gaactcaaga aggtaagcaa tggcagcgtg caaacaaaaa
     2221 agactataag gaaggccgtc cgaaaagaat tttaaatgat cggtacaagc atgcactaga
     2281 actgatgaag acaaacagca tgaaagaggt tgtaaagaaa actaatcttt ctttatctac
     2341 attaaaaaga ataaagaaac aagcaaagga agaaggaata tacaataatg aagaaaaaaa
     2401 gacagtttag taaatgcaaa tgggtgaggt atgcatttat tttagttttt gttctattaa
     2461 tattagtcgg tattccagct ttgtataatc acaaaatttt aacggaaaat cctagtaatc
     2521 ccagttatga cgggtggcta ggattttggg gaggttattt aggatcaata ctcagtgggg
     2581 ctatagcttt gttggttgta agactacaaa tagtagagga aaaaaatcgt cacagagaag
     2641 aaaagaatga tagtacattt tattatttat actctatgct agacaatagg aagtctgatc
     2701 ttatgaaaga taattctttt gaaaaactta aagaagaaat ctttagccaa ctcgattatc
     2761 agttaaaaga aaaagcagtt aattatatta ataacaaaaa aaatgtaacc atagtaaaag
     2821 aattcagaga taaattattc gatagtttaa ataaagaaaa aaatgaatag cttaaaagtg
     2881 tatctgattc agggatcaga tatcagcttg agaattattc caaaaatggg tttataaata
     2941 ctactgattg ggaaaccaaa tatctttcgt gttatattga tctcgaatct attgtaaata
     3001 agattgaaat tatagaaaaa gcaagtaagt atattaataa tagtaaacgg gtcaatatta
     3061 aatctgtaga aaatgttatt gaatgttatg aggaaatgag tgaattaagt aagattgatg
     3121 acggagactc aaaatacaaa agcattttac aagtactgaa agatgttaag gaaaaaaata
     3181 tcagtatttt agacgataaa caaagaaagg cagctataga agaagcattt atatgtatga
     3241 ctaattctgt cggtcaatat tttaagataa tatcaacgat tatccagttt tttaaaacta
     3301 atgatattaa gaaagaaaag gaactttttt acattaatag tttaagcgca gacatgttta
     3361 ttattgaaga gatccttctt ttctattata ttgaatatac ttctgatgga tttaacagta
     3421 aaagcaaact tcaaaattca ggaatattta aggaattgaa atttatagga aatga
//
LOCUS       DASNNU010000154         3441 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.159, whole genome shotgun sequence.
ACCESSION   DASNNU010000154 DASNNU010000000
VERSION     DASNNU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.159"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            255..1226
                     /locus_tag="U8116_002674"
     CDS             255..1226
                     /locus_tag="U8116_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN8435600.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            1258..2655
                     /locus_tag="U8116_002675"
     CDS             1258..2655
                     /locus_tag="U8116_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN8435601.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            2814..3437
                     /locus_tag="U8116_002676"
     CDS             2814..3437
                     /locus_tag="U8116_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435602.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1247 a          544 c          738 g          912 t
ORIGIN      
        1 ttaaaaaggc aaaaaatccg ccactttcag aaagcgtttt aaaaaagtgt tgcaatcttt
       61 taaaaaaatg ctatattaaa aatgaaccgg ttcaaatact tgtttaagta taattaatag
      121 tgagaggaag ttgctctact gaagacttta gttatatgga tattgatgac aagcaacact
      181 atgcaaagaa tgcggtcatc ataatcgaac aacaagaaag agaaatcaaa tgtaactggc
      241 aggagtgaga atgtatggca tcgatccgtg atgtggctaa gatggctggt gtttcagtgg
      301 gaacagtttc ccgttacttg aatggtcagc aactgaagga aaagaacatg aaaaaaatag
      361 cagaagcgat ttcagcgttg gactataaag aaaacatcat tgctaaaggg ttgaagaata
      421 atcgaagctt ctctattgga cttttgataa acgggatttc cagccgtttt gggtcggaag
      481 ttgtctcagg gatcgaaaga gtagctgaag aaaatgggta tagcttatta ttaagcgggt
      541 ttgccgatca gccagaaaaa attgaacaaa aaattgaata cctgatgaag catgtgatcg
      601 atgggctgat tgtcttctta tccgaagaag agtggtctgg atttgaaatg ctcacaaaaa
      661 tgagtattcc agttatcgca ttgaattgtc cagaaggacc agcaggtatt gatacaatat
      721 tagtgaatga tcgagaaagt gtagcaaaag tgatcgctca tcatggtgaa caaggacata
      781 aaaaaatcgg atttattgct gccactcaaa cagactacgt agcaagagaa cgtttagcag
      841 gagccaaaga agcggtccaa aatgatccat cgatccaatt agaggtcttt acaggagact
      901 attctagaaa aagcggctat tacggggcaa aagcattatt agaaaaaggg gcaactgcta
      961 tttttgtcag caattacaat atgtcgattg gtgccatcga atgtttcaat caagaaggtg
     1021 tcagaatcgg gaaggatatt gcgttcagtc actatgatta tttagataaa aacgatctga
     1081 gtatccttcc gaaaatcacg atcacgccac caacagataa aatcggggaa cgagcagcag
     1141 aacagctgtt aaaacgaatc aaaaaagaag aggaagaaaa tgaaaaagtg attgtgatgg
     1201 aaaatatcat acatggaatc aactaaaacg aactaaaaaa gaaaaagagg tgctttgatg
     1261 aaaaaatatc aatttccagc aaatttttgg tggggttctg cagcaagtgg cccacaaaca
     1321 gaaggaagga tttcaggcga tggaaaagga gagaacattt ttgattattg gtacaaaaaa
     1381 gaaccgcaaa agttctttga tcaagtagga cctgaaaaaa cctcgcaagt ttatacgaag
     1441 tatcaagagg atgtacagtt gatgaaacag actgggcata attcatttcg aacatctatc
     1501 caatggagcc gtttgatacc tgatgaatct ggaacaatca acgaagaagc cgttcgtttc
     1561 tatcatcgtt attttgatga gttgattgaa aacggtatcg aaccctttgt gaatctgtat
     1621 catttcgata tgccgatgta tctgcaagaa aaaggcggat ggttgaaccg agaaacggtt
     1681 gaagcctatg aaaaatatgc caaaagttgt tttgaattgt ttggttcgaa agtaaaaaaa
     1741 tggtttactc ataatgaacc aatcgttcca gtcgaagcag gttatttata tcgatggcat
     1801 tatccagaag agtcagatat gaaaaaagcg atccaagttg gctatcatga agcgttggct
     1861 tcagcgttag ccatcaaatc ttaccatgaa atggagcaag gtggtgttat tggtatcatc
     1921 ttgaatctga caccaagcta tccaagagac gaatcaaacc ctgaagatgt taaagcagcg
     1981 caaatagcag atgcgttctt caatcgttca ttccttgatc cagctgtaaa aggcgagttt
     2041 ccaagtgaat tgattgagat agtaaaagaa cttgatctca tgcctgtgat ccatgcagag
     2101 gatttgacgg ttatcaaaga aaatacagta gatcttttag gtatcaatta ttaccagcca
     2161 cgccgggtaa aagcaaaaga aacaccaatc gatactaaaa acggtccaat gcctgaggat
     2221 tatttcgaca attatgaaat gcctggtcgt aaaatgaatc cttatcgcgg atgggagatc
     2281 tatgaacaag gaatctatga tattttgatc aatgtacgag atcattatgg aaatatcgac
     2341 tgctttattt ctgaaaacgg tatgggtgta gaaaacgaag cacgatttat caaagaagat
     2401 ggcatgatcc acgatgatta tcggatcgaa tttgtccaat ctcatctgaa atatgtccat
     2461 caagccattc aagaaggtgc caattgttta ggctatcata tgtggacatg tatggataac
     2521 tggtcgtgga ccaatgcata taagaatcga tatggcttta tttcagtaga cttagcacaa
     2581 gatggaaaaa gaacaatcaa aaaatcgggt cattggttca aagacgtatc agataaaaac
     2641 ggatttgagg cttaagaaag aatagttgag agcaaatgcg tgaaattctc cattcttacc
     2701 attttgtgaa aaagaagttc aattatttac gatctttttc aacttttttg tctttctatt
     2761 tcattttttg tatataatag aatagtcaaa attaaatagg agtggaggaa agcatgagga
     2821 aaaaaagacc gtttcttctc ttttggttat tcggtttttt cttactgctt attttatccg
     2881 cagcattttc aggaaaaatg acagcagatc ttgctcagaa agaaacagaa aaaatcgtag
     2941 caagcaaaga atcagtagat aatttcaaaa aagaattctc aacttattgg tgggcggagt
     3001 ataaaataac tgccaattac gaaaaaggcg aattggacat ttatttgcca aaaggaaaag
     3061 aagaaaatca atttattaat atcgatgacg tacttagtgc gatttctctc agacaatatc
     3121 aatttaaaga caagcatttg accgttttgc ttttgactca tgatggcgac aaagtagccc
     3181 gtttgaaaaa tggaaaagtg atcaccgctt attctgaaaa agtagacaaa aaagtggtcg
     3241 ccgatcgttt agcggaatgg aaaaagacaa atgaacaagt caagaaaaca actgtctctt
     3301 ctcaaaaaaa cgagtcgaca ggaacaagtc cttcagattc tggcagtgga caaacgataa
     3361 cgggaagtga agaaaatcaa gcctcaatta ttacgaatga gtcatcacaa ggacaatatc
     3421 aatcctatgg acaatgaaaa a
//
LOCUS       DASNNU010000155         3399 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.160, whole genome shotgun sequence.
ACCESSION   DASNNU010000155 DASNNU010000000
VERSION     DASNNU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.160"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            269..481
                     /locus_tag="U8116_002677"
     CDS             269..481
                     /locus_tag="U8116_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435603.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(759..2558)
                     /locus_tag="U8116_002678"
     CDS             complement(759..2558)
                     /locus_tag="U8116_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN8435604.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2682..3278)
                     /locus_tag="U8116_002679"
     CDS             complement(2682..3278)
                     /locus_tag="U8116_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN8435605.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1244 a          611 c          596 g          948 t
ORIGIN      
        1 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
       61 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
      121 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
      181 atggataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
      241 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
      301 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
      361 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
      421 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctgctgaat tggtttctta
      481 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
      541 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
      601 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
      661 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
      721 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
      781 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
      841 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatagca
      901 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
      961 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
     1021 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
     1081 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
     1141 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
     1201 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
     1261 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
     1321 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
     1381 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
     1441 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
     1501 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
     1561 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
     1621 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
     1681 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
     1741 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
     1801 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
     1861 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
     1921 gtcaatgacc agctttcccg aatggctgct cgaaaaggac gatctcgtag aatcatttcc
     1981 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
     2041 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
     2101 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
     2161 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
     2221 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
     2281 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
     2341 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
     2401 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
     2461 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
     2521 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
     2581 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
     2641 tttaacattg tcaatcaatg actaatttta caaaataaat attatccaag aagaccactt
     2701 agatattgac ctccataaat caaggccata ctatataaga aaatcgacag tggtttagca
     2761 atcaaaatga ttagtgtata tcgtttcact gacatgtttg tcaatccagc catcaaacat
     2821 aacgcatcgt caggagcaac tggaagaaaa atagctagag caaacagctt ctcaaaacgg
     2881 ttttgattat ccagccagcc aatatatttg ttatatgtct tatcgctaat catggaaagt
     2941 atgaatggct ttccataacg acgccctagc agaaaaatga taatcgaacc aatgcagatt
     3001 ccaatatagt tatagataaa tccagcataa gggccaaata tcaggacacc agctgcagta
     3061 ctgatccctc caggaatgat tggaataact acttgcagga tttggatgaa gacaaaaata
     3121 atcggtccta aaatgatgga atcacctact aatcctctta acgcattcaa atctttgaaa
     3181 acacctaaat taataaaata aatcgtcagc gcgattgata ggcctaatcc gataatggat
     3241 atgaaattta tcagttttcg agaggcagca atattcatta ttcatcaact ccttagaggt
     3301 tctttgttaa ctatacttta ctagaaaagc aactaactgc gccatttttt gatatgacag
     3361 aaaacaaaca attttgtaag ttcatcataa gtataaaag
//
LOCUS       DASNNU010000156         3265 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.162, whole genome shotgun sequence.
ACCESSION   DASNNU010000156 DASNNU010000000
VERSION     DASNNU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.162"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(554..2167)
                     /locus_tag="U8116_002680"
     CDS             complement(554..2167)
                     /locus_tag="U8116_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN8435606.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="U8116_002681"
     CDS             complement(2308..3216)
                     /locus_tag="U8116_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8435607.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1052 a          559 c          574 g         1080 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa
     3241 agctttctat ttataattaa tgact
//
LOCUS       DASNNU010000157         3211 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.163, whole genome shotgun sequence.
ACCESSION   DASNNU010000157 DASNNU010000000
VERSION     DASNNU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.163"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..455
                     /locus_tag="U8116_002682"
     CDS             6..455
                     /locus_tag="U8116_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016612346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN8435608.1"
                     /translation="MLNVHCIEGRLAESPILKKTESGTCYTRVLLISKRNFKTKKESD
                     YQSDSFLCVLYGTIAKSFVEMTCRGSHVSFVARVQVRPYENQSGERCYVSEDIVQSYN
                     LLETKEVTNRRRKHLSEGQPAEEMALNEYRQAVEHIPTSYDEADWYQ"
     gene            468..776
                     /locus_tag="U8116_002683"
     CDS             468..776
                     /locus_tag="U8116_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435609.1"
                     /translation="MDKYHNIVIQRSIKQELKYKSLYILDLLIVFGPSMFLLYLQGYL
                     HFSLVTSILLGIVFFFLGLFLCAKPASNGNRRNLYTIYCLIVMDRNVYEVQSYKKRRG
                     "
     gene            780..1349
                     /locus_tag="U8116_002684"
     CDS             780..1349
                     /locus_tag="U8116_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016612345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435610.1"
                     /translation="MAAKQVQSFRSIGRKRNKGENPSIQELVPIKKMIRGNVVKKNGR
                     YLSFLKVTTRNINGLSASEQYQAMRHFENMLRIYEEEMSILSMMFPTNIDDNLVYWNR
                     QLQLAREERNTVRARIIREQISRLVWVEQELSNLEFYLMVYGKTEKEINLSKTILKRA
                     GGSNLGIKDIPPKQIEQILIKLNNLNTPI"
     gene            1367..>3211
                     /locus_tag="U8116_002685"
     CDS             1367..>3211
                     /locus_tag="U8116_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016612344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEN8435611.1"
                     /translation="MLNEKEIQTLVKEGFDLSFIEKIQPQGGITFPEDRMVAGDGTYA
                     CLHIYQLAKRPAPFWLTSIMNNPKTITKFDLSPIDKEVILSDIDKAMRELDSQAEENR
                     TRTQRNEAVNEFRSLERYANEINQGDEVSKMVDIRIYVTADTQEELEKEVGDLRRSLK
                     AMGYKSVSYIGKQAQEWLAFFQSYSEQQNEPGSKEGMTVPSSAIGGGYPFNHQFLNDT
                     GGGYIGKTDTDGPFIYNPYAITALRKSFSSMTLGKPGMGKSTLLKLLEEMLYGKRTII
                     RGFEKNRDWYKLIKSQNGKIYDLSGKEGMLNPMEPLATITDSSGKVVDELNSYLQHRA
                     TFFNKIRFLNPAMRSVDILDFGKIMDDFYISYGLLPENYTQNQKDIHIIGLDPSRYPT
                     VGEFKQFVDQFVESGYKDRVTDVKMVEMENFQTVITSMCEQYGSIFNGRSTFRNIDEE
                     DVLFFDIETIGHFDDEVYKCMLYTAMNIVWNQALKNGRKQKIALENGDITPDEVRFFA
                     FFLDECQEILSPDTVFVVKQVVKFLKEMRKFSAGAFFATQSPQELLPETSSDDYISKI
                     KQVFELCSSKFFLQLDASVSETMKKAMGNLLTDGQYEDLALLEQGEVIV"
BASE COUNT         1074 a          576 c          689 g          872 t
ORIGIN      
        1 atctcgtgtt aaatgtccat tgtattgaag gccgattggc tgaaagtcct atcttaaaga
       61 aaacggaaag tggcacgtgt tatacacgtg tcttgctaat atcaaaacga aacttcaaga
      121 caaaaaagga atctgactat caatcagatt cctttttatg tgtgctgtat ggcacgatcg
      181 caaagtcctt tgttgaaatg acgtgtcgag gatctcatgt gtcctttgtg gccagagtcc
      241 aagtccgacc gtatgaaaat cagtcagggg aacgttgtta tgtgtctgaa gatatcgtac
      301 aaagctacaa cttgttagaa accaaagaag tgaccaatcg tcggcgaaag catttatctg
      361 aaggtcagcc ggcagaagaa atggctttaa atgagtaccg tcaagcggta gaacatattc
      421 caaccagtta tgatgaagca gattggtacc aatagaggag gaaaaacatg gataaatacc
      481 acaatatcgt gattcaacga agtatcaaac aagaactaaa gtataaatct ttgtatatct
      541 tggatcttct gatcgtcttt ggcccctcga tgttcctttt gtatctacaa gggtatctgc
      601 atttttctct tgtgacgtcg atcctattag gcattgtttt ctttttcctt ggcttgttcc
      661 tatgtgcaaa gccagcgtca aacggtaaca gaagaaattt gtatacgatc tattgtttaa
      721 tcgttatgga tcgaaatgtg tatgaagtac aaagctataa aaaaaggaga gggtaaggca
      781 tggccgcaaa acaagttcaa agtttccgat caataggacg aaagcgcaac aaaggggaga
      841 acccttctat tcaagaattg gtaccaatta aaaaaatgat cagaggaaat gtggtcaaaa
      901 aaaatggccg gtacctctct tttttgaaag tcacgacaag aaatatcaac ggtctcagtg
      961 ccagcgagca atatcaggct atgcgacatt ttgaaaatat gttgcggatc tacgaagaag
     1021 aaatgagtat tttatcgatg atgtttccta caaatatcga tgataatctt gtctattgga
     1081 accgacagct acaactggca agggaagaaa gaaacaccgt tcgagcaaga atcattcgag
     1141 aacagatcag ccggttggtg tgggtcgaac aagaattatc aaatttagaa ttctatttaa
     1201 tggtatatgg aaaaacagag aaggagatta acctttccaa aacgattcta aaacgagcag
     1261 gcggatccaa tctaggaatc aaggatattc cgccgaaaca aattgagcag atcttaatta
     1321 aattaaataa cctgaatacc ccgatttagt ctggaggata aaaattatgt taaatgaaaa
     1381 agaaattcaa acacttgtta aagaaggctt tgacctcagt ttcatcgaaa aaatccaacc
     1441 tcaaggcggc attacgtttc cggaagaccg aatggtcgca ggggacggta cctatgcttg
     1501 ccttcacatc tatcaattag ccaaacggcc agcgccattt tggctaacat caatcatgaa
     1561 taaccctaaa acaattacaa aatttgatct ttcgccgatc gacaaagaag tcattttgag
     1621 cgatattgac aaagctatgc gtgaattaga ctcacaagca gaagaaaacc gaacccgtac
     1681 ccaacgaaat gaagcggtca atgaattccg atccttggaa cgttatgcaa atgaaatcaa
     1741 tcaaggcgac gaagtcagta aaatggttga tattcgtatc tatgtgactg cagatacaca
     1801 agaagagctt gaaaaagaag tgggggatct tagacgttcg ctcaaggcca tgggctacaa
     1861 atctgtttcc tatattggca aacaagctca agaatggcta gctttttttc aaagctattc
     1921 tgaacaacaa aatgaaccag gcagtaagga aggcatgacc gtgccaagtt ctgcgatcgg
     1981 tggcggttat ccctttaacc accaattttt gaatgatacg ggtggtggct atattgggaa
     2041 aacagataca gacgggccat ttatctacaa tccgtatgcg atcacagcac taagaaaatc
     2101 atttagttct atgacccttg ggaaacctgg tatggggaaa tcgacgttac tgaagcttct
     2161 ggaagaaatg ttatatggca agcggacgat cattcgaggg tttgagaaaa atcgagattg
     2221 gtataaatta ataaagtcac aaaacggcaa aatttatgat ctatctggaa aagaaggcat
     2281 gttaaatcca atggaaccgc tagctacgat tacggatagc agcggtaaag tggtcgatga
     2341 attgaacagc tacttgcagc atagagcaac ctttttcaat aagattcgtt tcttaaatcc
     2401 tgctatgcga tcagtagata ttctcgactt tgggaaaata atggatgatt tttatatctc
     2461 ttacggctta ttacctgaaa actacacaca gaatcaaaaa gatatccata tcattggatt
     2521 ggatccttca agatatccca cagttggtga attcaagcaa ttcgttgatc aatttgtgga
     2581 aagcggctat aaggatcgtg tgacggatgt caaaatggtg gaaatggaaa attttcaaac
     2641 cgtgatcacg tcaatgtgcg aacaatatgg gtcgatcttc aatggacgca gcacgtttcg
     2701 aaatattgat gaagaagatg tccttttctt tgatattgaa acgatcggtc actttgatga
     2761 tgaagtatat aaatgtatgc tttatacggc tatgaatatc gtttggaacc aggcacttaa
     2821 aaatggccgc aaacaaaaaa ttgctttgga gaatggcgat attactcctg atgaagttcg
     2881 attctttgcc tttttcttgg atgaatgtca agagatccta agtccggata ctgtttttgt
     2941 tgtcaaacaa gtggtcaaat tcttaaaaga aatgcggaaa ttttcggccg gtgctttctt
     3001 tgcgacacaa tcgccacaag agctattgcc tgaaacgtct tctgatgatt acatttcaaa
     3061 aatcaaacaa gtatttgaat tatgtagttc aaagttcttt ctacagctgg atgcttcagt
     3121 cagtgaaacg atgaaaaaag caatgggaaa tttattgacc gatggacagt acgaagatct
     3181 agcattactc gaacaagggg aagtgatcgt c
//
LOCUS       DASNNU010000158         3203 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.164, whole genome shotgun sequence.
ACCESSION   DASNNU010000158 DASNNU010000000
VERSION     DASNNU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.164"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            668..763
                     /locus_tag="U8116_002686"
     CDS             668..763
                     /locus_tag="U8116_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN8435612.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1066..1311)
                     /locus_tag="U8116_002687"
     CDS             complement(1066..1311)
                     /locus_tag="U8116_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435613.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1656..1940
                     /locus_tag="U8116_002688"
     CDS             1656..1940
                     /locus_tag="U8116_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435614.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1943..2359
                     /locus_tag="U8116_002689"
     CDS             1943..2359
                     /locus_tag="U8116_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435615.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2496..3173)
                     /locus_tag="U8116_002690"
     CDS             complement(2496..3173)
                     /locus_tag="U8116_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN8435616.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
BASE COUNT         1065 a          598 c          500 g         1040 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tacccttctg tatccgttgg gagagtaagg ctcaactgcc ccctaaaaag
      241 ggttctaaaa aatctttaaa aaataaataa aataaacgcg cgcgcgcgag caaaatttcc
      301 cgccaaaaaa tcacccactt tttaaaataa acaggttcgc gcatgatatt aaactgccaa
      361 tctagctccc tatggtcgct gtaagaagga gtgatcgcta cgctctcaca ttttacttgc
      421 agcagtcgcc tttaggctcc ttcgccaaaa ataattcatt agggactcct ttgtggcgtc
      481 ctgagagctc gtctaagccg ttctaagaca agattcgata aaaactatgt ccttcgttct
      541 aaagcgcctt acaggcggtc agggcaagcc ctaacaaccc caaggcaata aaaaacttag
      601 gcttattttt gcttttttat tgtataattg attgtcgcct atccgacagt cttgcgactg
      661 gaaggaggtg tgtttcgtgt tcactaactt tatttgctcc cttcttgttg ggcttttaag
      721 cgctttgttc agttactggt tgaataaccg aaacaagaag taaaacttaa ttaaggcgac
      781 tcagcccaca aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtgtttcgtg
      841 ttcacttatt tgcaatcaaa ttataacaga aataaatatt tatttcaaaa atatcctaca
      901 aaagtctaaa atccgtaatt atttcttata tgcaacattt acaaataaag tcgctctgag
      961 agctcgtatt tttcgattta agacgtttta tttagttttt ggtgtatttt cattggtcca
     1021 agctaaaaaa ctcttagaag cgattttaga gccatttaaa cccacttaat tatattctcc
     1081 atgaaattcg ctccatgcgt caaaagctgc gtctgaaatt ccgccgtctt cgttcactag
     1141 ttgcattaat ttttccaacg atcgttttct ttcgggtgtc atatttttat attccaacaa
     1201 tttttcgtct gttaaatcga acttgattgc ttgaattgct tgctctaatt tttctaagtt
     1261 ttgaaaatat tcgtaaggaa atttttccat tttttctttc tccagtctca caatttttct
     1321 agaaataaaa tcacttcttt gccgtctata ctctcaaaaa aaatgtcgtt tgaaaaacta
     1381 aataaatttt tgatcgtgca tttttaaatt tggttatcga ttacacgtgc agcactcccc
     1441 cccgaatttt aaagagcata aggaaacgtg tacccacttt ctaaaaaaag tgggtagata
     1501 ggctgtgcct atcacgtttc tcagaaaaaa atttgatatc actttttcgc catcgtgggt
     1561 agcttatgta tagcattttg gcaaaaaaca gctatacaaa tatagtattt gtatggtata
     1621 cataaattat acataattgc aaaaggagat tttttatgga cattttagtt cggaatattg
     1681 atccaaagtt tgttgcaaca atagacaaac gttgccaaga actcacgaat aaaacaggag
     1741 aaaaatggag tcgaaataaa tatctaaaag ttttaatcga aagtgatttt gaccgtcaac
     1801 ttacgactta caaaaaagac caattcgatt tattgctcga taaatttatt gctatccaaa
     1861 cagaaaatac tcaaatatta agcgagtatg tctacacaaa taatcaaatc attgaaatgc
     1921 ttatcggagg aatgatataa ccatgagaaa aacttctgtc caaaaacatg ttactttgta
     1981 ccctagtcat gttgacgttt tagattccat catcgaaaaa aatccagata tcaataacta
     2041 ctcggaagcc attcgctttt tatgtatgag ttatgaaccc gataccaaca cgaccactac
     2101 cagcaatcag gcaaaactaa atgctatggg aaaagaaatt tccatcatga ctgaaatcat
     2161 cgcagaattt gctgatttat atctaaaaga tacaggcgtt cttattggca caaattctaa
     2221 agtatatcaa gaagtgaaag aaaaaatcga agctaaacta aatccatcaa atcgaaatcg
     2281 accaaagaat catcaaaaag aaaccagtca aacagtcact tctagaaatt tcgacccaac
     2341 aaattttcta aaacgataaa aaacagatag gagttattcc tatctgtttt ttatttagaa
     2401 gccccataca atatgctaga atgtactagc aatcttagtc ggttgtaagg gacttgtcta
     2461 cttgcttaat gtaagggggg cgagttcttt tttatttatt cttctaacca gttacgcaga
     2521 gaaatctttg gcaaatctga cgttaggctt gaaaaagtaa attctaattt ggcgatttta
     2581 ttgccctttt ttgcttttat ttttgtgact ttaagatcat tgaagtattt ttttagttct
     2641 cgtaatgcag gatttaatac tttttgatca atgttaccca tttgatagta ttctgggata
     2701 tccattaagc tcctaaactc ttcaatacga actacataat aaccagtaga acggtactgc
     2761 ataagcagtc ggaatagagt ttttgcatac gtagaacgca acgacgtgaa agcagataat
     2821 tcaaatctac taaattcagc tgttaaacca ttaataatat attctaggtc cggattaaca
     2881 ctgacttcaa caaacttttt tgttttattg atttcaaaac cagtaaacaa aacaaagtag
     2941 cgaatagaat cctcatcttc agtacggtaa gtaagattaa gcatatcttt atagagcttt
     3001 tctaaatcac cgacaaaggc ttttgtagca gtcatcttat aatcacttaa ctcttttaat
     3061 tcctcgaaag aaaatctaac ttttttcaaa ccacgatctt tcatacgagc gcataatgca
     3121 aaaaataaat ccatctgctt agcattgaaa tttttcagcg gtactaaatt caattcattt
     3181 tgatatttga ctgttaattc cgt
//
LOCUS       DASNNU010000159         3120 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.165, whole genome shotgun sequence.
ACCESSION   DASNNU010000159 DASNNU010000000
VERSION     DASNNU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.165"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..218)
                     /locus_tag="U8116_002691"
     CDS             complement(<1..218)
                     /locus_tag="U8116_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435617.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFK"
     gene            complement(654..1220)
                     /locus_tag="U8116_002692"
     CDS             complement(654..1220)
                     /locus_tag="U8116_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN8435618.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1220..2176)
                     /locus_tag="U8116_002693"
     CDS             complement(1220..2176)
                     /locus_tag="U8116_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEN8435619.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2176..2832)
                     /locus_tag="U8116_002694"
     CDS             complement(2176..2832)
                     /locus_tag="U8116_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN8435620.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          980 a          604 c          550 g          986 t
ORIGIN      
        1 tttttaaact tagtctctac ctctaatata tatcgagttt ttattttgcc ttcagagaat
       61 actgtttctt tttttgcagt tacaggttgt ttattttcgg aaaaagctaa tatagctaaa
      121 attgaaacat ccatctggaa tttatctttt ttactgcttt gatcataaaa gtctgcatat
      181 tcatcactga tattttttct aataaattct tccatcataa aaatcacctc aaaacgatta
      241 tacgaactta cgttcttgtt gtaaagcgat atttgagggg caaaaaaggg gcataatttg
      301 actgatatta agttagctat tgatatatga atgacataca aaaatataaa atcttttaaa
      361 aatgcttata tatcaatgtt tttgccattc ttttatatat ctgttatatg tcaaaaaaat
      421 gtgttcaatt tcaatttgta tttgaataaa gaggttgtga aaaagctgtc ctgcatcaag
      481 taataagaac agccaatcaa aaacagcttc tcatgttttt gatttggttg gacttattac
      541 cgcagatata agcttttgag caccgtgaat aaagaggtta tgaaatcaac gctctaacct
      601 gagtattaga gaaaaaaacg caaaacatat catttatgtt ttgcgttttt tatttatctt
      661 tttttctcaa tacaatataa aataggcggc tggttctttt ggttgataaa ccgataattc
      721 agcacattat atgtttcctg tggcagtttg gaacaaaaag aatcaaccat atccagttct
      781 ttttctccac cttcatgacc atagtagacg acaaggatca atcgtccgcg aggcacaagt
      841 cgtttcaata tttcttccat agctgtgcgt gtagtctctg gcatagtgat gattgcttta
      901 tcactttttg ggagataccc tagattgaaa atccctgctt tgagattttg ttgttcagct
      961 atcacttctc ctaacgtttc gtggccttgt agaaacaatg agacacgttc ttctaactga
     1021 cgctgttcta gcctttcacg agtagcatcg attgcttgtt gctggatatc aaaactatag
     1081 acatgaccag tttttccgat gtgttctgct agaaaaaccg tatcgtggcc attgcccatc
     1141 gttgcatcta cgacgtgatc ccctggttgc aagatttctt gcagtaatgt atggctgaaa
     1201 tgcaaagcag tttgcaacat cagacgctca cctttttccg tacatcatat tttccttgga
     1261 agctatctct gcgaagaagt tcttcatcaa ttgcattcaa gacttcccat tttttcaagc
     1321 tccacattgg tccaatcaag gaatcccacg gcgcatcccc tgtcaaacga tggatgatga
     1381 tctcttttgg tatcatttcc agctggtcgc aaatgacatt gacatagtca ggacggctca
     1441 ttaattgaag gcgaccttca tgatagtccc gcaacattct cgttttgcgc atcagatgca
     1501 tcaaatggag cttgattcct tggatatctg aatctaaaat ggtgcgtctg acattttctt
     1561 tcatcatttc taaactttcg cctggtaatc cgttgatcaa atgcgtacaa acacggatat
     1621 tatgtttgcg taatttagcc acgccatcta aataagtttg ataatcatgt gctcggttga
     1681 tcagttttga agtttcttca aaagttgttt gcaatccaag ttctacccat aaatacatcc
     1741 gctcgttcag ttcagcaagg taatcaacta cttcatctgg cagacaatcc ggtcgtgtac
     1801 cgactgataa tcctacgaca cctggtaaat tgactacttg ctcaaatcgt tctttgatta
     1861 tttcaactgg cgcatgggtg tttgtaaaat tttggaaata aacgatgtat tgttggacat
     1921 gaggccattt ttgatgcatc atgtcgattt ctttctggaa ttggattgga agagggtctt
     1981 caggtgcaac gatcatgtct cccgaaccag atacactgca aaaagtacaa ccgccatggg
     2041 caacagtccc atctcgattc ggacagtcaa atcctccatc gatcggcacc ttgaaaattt
     2101 tcccgccaaa ctcatttcgt aaagcgtaat tccaagaatg atatctttta tttccttctg
     2161 aataaggaaa agtcattaaa agtccttctt tctgtaagag ttatctattt gttttctttt
     2221 ttctctatac actggataag tcaggcacag ccaagaaaga ctgagtaagt aaccgccgat
     2281 gatatcagta ggaaaatgaa cgcccagata aatgcggctt atcccgacac ttactataat
     2341 aaacatcatc agcaattgaa gcaatgtgcg taaggttctt ttttgaacaa aagctggaag
     2401 cagaaatagt aaagaaccgt aaagaatcat actgcctgct gcatgtccac tagggaaact
     2461 gtaacttgtt tcaactacta agtgcgccaa tgaaggacgc tctcgcatga aaaatagttt
     2521 gatcaccggg ttgatgattc cagcaattac aataaatcca gaaacgaacc aaaatgtttc
     2581 tgcccttttt ttcttatacc acatccataa agccgcacaa gcaaataaaa tgatgacggt
     2641 aagaggatta ccgaatttag tcataaagag ttggtagctg ttcagatcag gataagaagc
     2701 tctgacaatg cctgtaaacc actgatcgaa accagaaagc cacgacagat ggaactttac
     2761 gatccagccc aaaatgaaaa acaggacaag aaagaagcta ccagccacct gaaatgatgt
     2821 tttatatttc atacaattcc tcctttgaag caatgactta ttatagcatt tttggtccag
     2881 cgaaaaaaac ggtaaagaga taaaaccaat gtatgattcc tctttgaata accaatattc
     2941 ttataaaaaa aataactgta atcggaaaga cattttttgg taatgcaaat gaaatgttag
     3001 ggaaataaaa tcagtgtttt tcgaaatcct tttaaacaag aacgtagacg taggaagaac
     3061 ttgaaaatga acagaagttg ttcattacgg taaattacaa tatgattaca aaaaacaaca
//
LOCUS       DASNNU010000160         3038 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.166, whole genome shotgun sequence.
ACCESSION   DASNNU010000160 DASNNU010000000
VERSION     DASNNU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.166"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="U8116_002695"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="U8116_002695"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEN8435621.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="U8116_002696"
     CDS             complement(1034..1954)
                     /locus_tag="U8116_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN8435622.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /gene="xth"
                     /locus_tag="U8116_002697"
     CDS             2067..2819
                     /gene="xth"
                     /locus_tag="U8116_002697"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEN8435623.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          856 a          612 c          526 g         1044 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagat
//
LOCUS       DASNNU010000161         3035 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.167, whole genome shotgun sequence.
ACCESSION   DASNNU010000161 DASNNU010000000
VERSION     DASNNU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.167"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            148..942
                     /locus_tag="U8116_002698"
     CDS             148..942
                     /locus_tag="U8116_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8435624.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            932..2974
                     /locus_tag="U8116_002699"
     CDS             932..2974
                     /locus_tag="U8116_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN8435625.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT          979 a          486 c          608 g          962 t
ORIGIN      
        1 aaacttgtag ctggtggatt tgttttttgc atatttaatt ttgctgaata aaataattga
       61 ataaaatttt ttattcagtc caagtaaaag tgacaaaaac ataagagcaa attattcgta
      121 gatatgctac tatgcaaata aatagaaatg aaaaatgaaa gaggaggaga agaattggaa
      181 aagatgatcg aagtgaaaca tctaagtaaa agctacggca gcaaactttc tcccgtcaaa
      241 gccttagata atatctcatt cacagtagaa gaaggcgagt ttgttgggat tatgggccca
      301 agtggtgcag gaaagacaac gctgatgaat atcttgtcca cgatcaattt gcctacgatg
      361 ggcagtgtct cgatccaagg aaaagaaatc acaaaaatga aaaacagtga gctcagtgac
      421 tttcgtcgaa aaaaactagg attcattttt caagagttca atttgatcga taccctaaat
      481 gcaaaagaca atatcctatt gcccctagca gttgaacgga tgactaaaga ggaaatggaa
      541 aagagagtaa gacatgtagc tcaattgttg aatatagaag aactgctcaa acggtatccc
      601 gatgaacttt ctgtcggaca gagacaacga attgcagctg ctagagcact tgttgttcag
      661 ccacaagtga tttttgcaga tgaaccaact ggctctttgg actcgaagtc ggctacagaa
      721 ttgttgaact acttgaagac catgaatcaa aaagaaaaag caacgatact gttagtcacc
      781 catgatccat atacagcaag ctactgcgat agaattttat ttattaaaga tggtgtgatt
      841 ttttctgaag ttgtcagaag agggacaaga agagagttct ttgagaaagt aatagacatg
      901 caggcgacga ttggcggagg aggaaaaatg aatgctgtat aaactagccg ttaaaagttt
      961 ttttcggcag tcacgaggct atcttgttta ttttttcagt ttgactttat ccgtaatgat
     1021 ctattattcc ttcagcgcaa tgacctacga tcaatcttta gtcagaagag caagtcaaga
     1081 tacttcgata gatggtggat tgagttttgg tggatttatc attactgttg ttttgctttt
     1141 tttcgtattc agtgccaacc gatttttctt gaatagaagg caaaaggaaa taggcatcta
     1201 tcagcttttt ggtatgaata aactgcagat atcaggtata tatgtgttag aaatcatgat
     1261 aattggattg ttcgcctgta tttctgggat attactcggt attatttttt ccaaactctt
     1321 ttcaatgatt ttggttcgaa tgatggatat ggatcttact agtccatttt ttatctccat
     1381 tccatcagta gtagatactt tatttgcttt tttcatcatc cttttagcag tatctgtata
     1441 ttcaatatgg aaaatatggc gctatccaat gattcgaagt tttggagaaa aagaacaagc
     1501 agatagttca acaatgcgct tcaggacaag gcaccgatta ctcgcagtca tcgggttact
     1561 acttttaacg agcggatatt ttggagctag tcattttagg gaaatcactt catggatgat
     1621 cagttatcat tatcttgata cattagtttt cttttttcct tttttaattt ttttcgtttg
     1681 tgtggtcggt acttatttgt ttttctgtta tacatttcgg ttatttttga tcatattgag
     1741 taaatggaag atttattact atggtatcaa ttttttgata atgggtaaca cgcaagttca
     1801 tttgctcaaa agttggcgta caaattctct gattacagtc attctcggaa tttctttaat
     1861 gacaattggc ggcacggcta gtatctctac tattttatcc taccaggaga aagtttcctc
     1921 cccgatggac tatcagtttg atgttgagac agagaaaaaa atcaagccga ttctagcaga
     1981 ggaagggcag aagataacta aagaaatcac cttgcattat aaagtcattg ggagtatcta
     2041 tagtcaaaat ctcgaagaac ctatgtttca aactgctaat ctgatcacgg aaacacaata
     2101 tcaagccttc agaaaagtta atccagattt gccgaacatt tctttgaaat cacctggtca
     2161 tactgttttg cttgatcaaa tgcaaactct ttttcggaat atttcattat atggatctag
     2221 tattggactg ccagataatc aagaattgaa gattcaacaa atgcttccaa gtgtattagg
     2281 tgacgaaaca atggtatatc taagtcctgt actgattgta gatgataccg tttttgaaaa
     2341 agcaaaaggt gtggattatc aagtagtcaa tgtagatgta tcaggtggaa ataatgaaaa
     2401 aatagatgaa cgactgagca atgacctgtc gataaagtgg ctcaatgctg tttattattc
     2461 ttatgatatc gtaaatggaa gtctacaagg agaaattagc acaaagaaac ttccggatga
     2521 ccaaatgaag gaagcacaat ttagccaaga aagctctcgt ttgaatttct cttccagcta
     2581 ttcgaaattg cgtgcagtta atcggcgaat cggtatcaat atttttgtca cactatttgt
     2641 cgggatgatc gtgattattg ctactggtag tattttgaca gtccggcaat tagcagaagc
     2701 agaggaagaa aaagaaaatt atcacctatt gactaagctg gggattccac aaagaaggat
     2761 cagacggatg atcttcgagc aaaacgcact taccttcttt ccacccatga tattagggat
     2821 cactcacgca gtatttgcaa tcaatgtctt cactcagtat ataaaaactg ccgattactg
     2881 gttggcttat cttgtgagtg gcttattgat ccttatctat cttttgcttt atgtcattac
     2941 gagtcagtta tattgtcgga ttatcgagga atgatatacg gaataagaca agataataaa
     3001 tagaactaaa ttttgaatac catttatttg gaata
//
LOCUS       DASNNU010000162         2817 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.168, whole genome shotgun sequence.
ACCESSION   DASNNU010000162 DASNNU010000000
VERSION     DASNNU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.168"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            225..1676
                     /locus_tag="U8116_002700"
     CDS             225..1676
                     /locus_tag="U8116_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN8435626.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            2025..2657
                     /locus_tag="U8116_002701"
     CDS             2025..2657
                     /locus_tag="U8116_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEN8435627.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT         1068 a          402 c          533 g          814 t
ORIGIN      
        1 aacaaattac attagacaaa ttgaaaatgt tagattattt atttcatcct agttttagcc
       61 aaccctataa ttttatttta aaggcagtaa caggattaaa cattgaaaca taatcgttac
      121 taaatgaaat caagaaatat actgtattac tcaattcatt gaaaatattt atcataattt
      181 ttaatagaaa acactattaa agagaaaaaa gagaggtaaa tcaaatgaaa aaatctttaa
      241 aacttgcatc agcagttata ttaatgtccc aactccttat agcaacacct ttccatgtgt
      301 tggcaagcga aaattcaaca tacaaaattg caaaagaaag aaccgagacg actgaaatga
      361 gtaattccga tgtaacagca gagagtagtg aacttttgac aacttcatca agtgaaagta
      421 gtacgacttc aaatgccgag tcttcaacaa cttcatcaag tgaaagtagt acgacttcaa
      481 atgccgagtc ttcaacaact tcatcaagtg aaagtagtac gacttcaaat gccgagtctt
      541 caacaacttc gtcaagtgaa agtagtacga tttcaagtac cgaatcttca acaacttcaa
      601 gtagtacaag tgaaataact tcttcgaaag tgacaaaacc aaaccatata gaatcttcaa
      661 aaaaggaagc gccaaaaacg gaaggacaaa caaaaacttt ttcgacacca cagcaaatag
      721 aatcaacaga atctttccaa tcacaaccaa tactatctgg taatgaagaa attcattttg
      781 acaaaaatga atctgtagaa aattttatta tgaaaattgg tgagtctgcg agaaaaattg
      841 gtcagaaata tgatttatat gcttctgtta tgattgcaca agcgatctta gaatctgcca
      901 gtggtcaaag ccagttagct caggcaccga attacaattt atttgggata aaaggaactt
      961 ataatggaaa ctttgtcatt atggtaacaa acgaagattt gggtaatgga acgctttata
     1021 cgacccaatc aaaatttaga gtgtatgaaa actatgagga aagctttgaa gattatgcca
     1081 aattattaac taagggaatt agtggaaata aagattttta tgcaggagca ttaaaagcta
     1141 atagtaaaac atatcgagaa gcaactaaat tccttacagg acggtatgca acagatacac
     1201 aatactattt gaagctaaat gaattaatta aaacctacga tttaaccaac tatgataaag
     1261 aagttaaagg tccacaaatg agtaagactg gataccaagt gcctattcat aattattcaa
     1321 taaccagttt gtttggaaat cgtggaaatg aatttcaccg aggacttgat atggcagcta
     1381 cacagggtga gccgattcat gcagttaaag ctggtaaggt tgttaaagca gagtttcact
     1441 actcttgggg aaattacgtg gtgatacaac acgaagatgg atcaaccgcg ttatatgctc
     1501 accagcaaca atacctagta aaaactggag ataatgtttt gcaaggacaa ataattggct
     1561 atgtaggaag tactgggaat agtactggcc cacaccttca tttggaaatt tgcaaagata
     1621 gtagtctttc tcagagcatg ttagttgatc cgaaaacctt attattcgga gactaaaaaa
     1681 ggtaatcaat ctgacaaagt aacaagaaaa tggttggatt cttagacctt tgatcaggat
     1741 attgagactg gaattgatat ctcgattacg atgggtttga caaataaaac aagtccatta
     1801 tcgaatatcg agtgtattat tgccgtcttt atgctcatat tttgagcagg tttcactaga
     1861 tgaaaacaat ttatctatct tgaggatttc gtgtacgtat cgttcagtct tgtattgtaa
     1921 attagtcata aaatcagact aagaaaaatt ggaaatattt ttttctgatt tattaaaagt
     1981 taattgaaat tatatggtac ttctttcttg ggagggtagg tgcagtgaag gaaagatatg
     2041 gtcaccttac tgttgttgag gagattactc ataatggata taaaaaatat cgttgcctat
     2101 gtgactgtgg gaatgaaacg attgtatatc gatcaaactt actatcaggt agaaccaaaa
     2161 gttgtggatg tggaaattat aaaaataggc ataaatataa aaatttgaca aataaaactt
     2221 ttggtaagtt agttgcaatc aagccaacta aactgcgaag tgaaggaact gttgtttggg
     2281 aatgtcaatg tctgtgtggg agaaaaaagt tagtatcggc aaagcgactt ctaaaaggtg
     2341 atgtaaaaag ctgtggatgt ttgaaatcaa aaaagcatca cattgaaaat aaaaaattcg
     2401 gattacttac tgcaaagtat cctttggaca ataactgttc atggaaaaca aaatgggttt
     2461 gtcattgtga gtgtggcagt atgtgtgaga taagttatag taacttattt tttggacata
     2521 ctcaaagttg tggatgttta aaaaggcaag aaacaaggac atgtgttgaa gggacagtaa
     2581 tagagtgtct aacgtcaaaa ttaccgaaaa ataatacaag tggtgttaag ggagtttatc
     2641 gttctaaaga gaaatagatt gcgtatataa ctttaaaaaa aaagaggtac tatttaggat
     2701 cgtatgattc tattaagagt gcagctcaag ctagaaaaga tgctgaaaat aaattgtttc
     2761 aacctttgat tgacaaatat cgaggtaatc attaattatt gtggataata attaaca
//
LOCUS       DASNNU010000163         2766 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.169, whole genome shotgun sequence.
ACCESSION   DASNNU010000163 DASNNU010000000
VERSION     DASNNU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.169"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(795..2135)
                     /gene="glnA"
                     /locus_tag="U8116_002702"
     CDS             complement(795..2135)
                     /gene="glnA"
                     /locus_tag="U8116_002702"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEN8435628.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(2163..2555)
                     /locus_tag="U8116_002703"
     CDS             complement(2163..2555)
                     /locus_tag="U8116_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN8435629.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          815 a          599 c          452 g          900 t
ORIGIN      
        1 agaactactt tttcctactt gcagcaacag tttatatagc ttatcgtaga aacaatcttt
       61 ttctttcacc cttatttacc tcctttacgt aaaaaacgta gtccacagtt ctcggatttt
      121 tggcatatct tttctattaa ataatatcaa aaacaaaaca attccactaa ttttttcaaa
      181 gatgaccttt ttttcaacaa aagtgccttg tagggattca ttgaaggtaa cgaatcacat
      241 aagtaaaaaa taaggagatg cgacaaaagt cgtgcctcct tatttctaaa taaacggtgt
      301 tctcctgcca actcctcggt cacaagtcac aatatccatc aaacatgaga agctgttttt
      361 gaaattattt cttgtgattc ggagttaaat ggtaggctca caacctctgg ataaacggta
      421 ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtaga aagccatttt
      481 cacaaaattt ttcttaatac tcgaaactaa acgtcttttt caaaacctct tggtaaacgg
      541 tgttcaaaag cttgcatctg cggtaataag ttcagccaat tcaaaaacaa gaaaagctgt
      601 ttttgcttgg ctattcttat tacttgttgc agggcagctt tttcacaacc tctccactaa
      661 acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat atgaggaact
      721 atttttccgt tagtttctct aatactcagg tcaaaacgct gatttcacag cctctaattc
      781 acggtattcc tgttttaata aagttctaga tattgttctc tttcccactc ggaaacagtt
      841 tgtctgaatg ccgcccattc gagacgtttt gcttcaacaa aatttgagaa gatatgttgt
      901 cctaatgcgt caatcattac attatcctta cgcaattctt tgatagcatt atggatagtt
      961 gaaggtaagt cctggatatg tgcagcttct ctttcttcct catccattac ataaatgtta
     1021 cggtcaacag ctgctggcgg tgtgatctga ttacggatac cgtctagtcc tgcttgcaac
     1081 aatactgcca ttgccaagta tgggtttgca gatggatcaa ctgaccgcaa ttccaaacgt
     1141 gtggataaac cgcgagattc tggtacacgt actaatggcg aacgattacg gccactccat
     1201 gcgacataaa caggcgcttc ataacctgga acgagacgtt tataagaatt gactgttgga
     1261 ttgcaaacgg ctgtgtaggc acgagcatgt ttcagcaaac cacctaagaa atgataagct
     1321 gtttcactta gttccaattc accgtcttta tcatagaaaa catttccttc atcattgaac
     1381 aaagacatat tgcaatgcat accagaacca ttgatcccaa ataacggttt cggcatgaaa
     1441 gtcgcgtgaa gtccatgctt gcgtgcaatc gttttgacaa cgagcttgaa ggtttggata
     1501 ttatcacaag catctaccac atctgcatat ttaaagtcaa tttcatgctg tcctggcgct
     1561 acttcatgat gtgaagcttc cacttcaaag cctagacttt ctaattccaa cacgatgtca
     1621 cggcggcaat tttcacctaa atctgttggt gcaaaatcaa aataaccacc tttatcattc
     1681 aaggtcgttg tgatcccacc gtcttcgtct aatttaaata agaagaattc tggttcaggt
     1741 cctaaattga aagaggtaaa accaaggtcg cgcatatctt tcaatgctcg tttcaaattc
     1801 ccgcgaggat cgccggcaaa tggtgtccca tccggattat agatatcaca aattaaacga
     1861 gcaacttttc catgagcact ttcccacggg aagatcatcc aagtggagag atctggatac
     1921 aaatacatat cgctttcttc aattcgaaca aatccttcaa tcgaagaacc gtcaaacatc
     1981 attttatttt ctaatacttt gtcgatctgg ctcactggaa cttctacatt tttgatcgta
     2041 cctaagatgt ctgtgaacat caatcgtaaa aatcgtacgt tttcttcctc aatgattcgt
     2101 ttgatttctt cacctgttat ctgtttcttt accatggtat ccctcttcct tttttaattt
     2161 attcagtcaa agaattgtct ttgcaatcct tgcgactgat aatgacctgt tttgcttaac
     2221 ccaccttgcg ccaaaatctc atcacgaaaa atttgtcgaa cgtcatgatc tgtcaacggc
     2281 ttcttgtttt ttgcttcttg ttttttcgcc tgtgccattt cgtatacacg tttgatccca
     2341 gccatattca atccctctga aagatagtct ttaatctcaa gcaacatatc aatatcattc
     2401 agcgaataca tacgtcggtt tccttcgctt cgttccggat gaactaaatc ttgttcttca
     2461 taatagcgaa tttgccgagc agacaaatca gtcagcttca taactgtacc aattggaaat
     2521 acggacatag atcggcgcaa ttccttttct cccatcagac gtcccctttc gttttataag
     2581 ttggctaaag tgtaccaatt aaaaaaaata ttgtcaactg catatgttag gttttctaac
     2641 atgagatctc tattttttat aaaataacaa aaaaataaga acacaaaaaa ggtatctagt
     2701 tgattttcgt accagatacc ttttattttt tattatactg tactatcctt tattagtgtt
     2761 tttatt
//
LOCUS       DASNNU010000164         2685 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.170, whole genome shotgun sequence.
ACCESSION   DASNNU010000164 DASNNU010000000
VERSION     DASNNU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.170"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1412
                     /locus_tag="U8116_002704"
     CDS             <1..1412
                     /locus_tag="U8116_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN8435630.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>2685
                     /locus_tag="U8116_002705"
     CDS             1546..>2685
                     /locus_tag="U8116_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN8435631.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISF"
BASE COUNT         1054 a          370 c          536 g          725 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gcttt
//
LOCUS       DASNNU010000165         2637 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.171, whole genome shotgun sequence.
ACCESSION   DASNNU010000165 DASNNU010000000
VERSION     DASNNU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.171"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(40..951)
                     /locus_tag="U8116_002706"
     CDS             complement(40..951)
                     /locus_tag="U8116_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN8435632.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1328..1966)
                     /locus_tag="U8116_002707"
     CDS             complement(1328..1966)
                     /locus_tag="U8116_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN8435633.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1970..2497)
                     /locus_tag="U8116_002708"
     CDS             complement(1970..2497)
                     /locus_tag="U8116_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEN8435634.1"
                     /translation="MKNSTYWERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDN
                     LQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIF
                     RSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFI
                     LSTEKKSQLLLMNGG"
BASE COUNT          759 a          502 c          396 g          980 t
ORIGIN      
        1 ttattgattt aaatagttca tattttttat ttataccgct tatttactac ttgctgtact
       61 agaatctgaa gtgaatgtac tcaaaacatt ttcaaagtct ttgtctttga tttttacatt
      121 agcgtctttc aatacttcgc caattacttt attctggaag gtactgtcac ttaattttgt
      181 gtctgttgca atttctttca attctttttt gtatttatcc atgtcgttgc ctttgttttg
      241 gtttttcacc attttaacca cataatattc cgtagtataa gttgaagcat tggtagaagt
      301 gatcacatca gaaacttgac catctttcaa cttaaaggct gcttctttga cttctgccgg
      361 aaccgttgta gtagtggaat caaatttcac ttttccgcca tcttcttttg tcgtagtatc
      421 agttgacttg tctttggcta atttagagaa atcgtctccc ttatcagcag ctttcttcac
      481 atcttttgca tcatcttcac ttgctacttg gataatttga gcttccactt ctggatggaa
      541 agattcccaa gccgttttta aatcttcatc ggtcaatttg atatgtttct ttagaccttc
      601 ttgaaaggct agactttgtt tgatactgtc tttgaatgtt tgttctgtgt aacctgcaga
      661 tttcaattgt gaatcaaatg aatcacccaa ttgtttcttt gtttgatcat attgggaatc
      721 gatttctttg gttgatacgt catctccata tttatcttca aatacttgat aaacgatcaa
      781 ctgttgcaca gcggaatctg ctccttgcat ttttttcaag ctatccatta cttcttgttc
      841 gctgattgta ccgcctttat aactaacggc tgttttttca ccggcattcg tacagcctga
      901 caataagcca gtgcctagta cgatggcaag tgctaaaaat ctttttttca tttatcaatt
      961 ctcctacttt tcgttttttg cgaattgcta aaaacaaagg tgttcaagat gaaaatcatc
     1021 aagaacacct ttactaatct gctcgatacg tcctgaatag atataaggct acacaatcca
     1081 gaaagttcgc tcagcagaat aaatgtcaag tagcacatca caccttaata tactacttct
     1141 tttgttaccg tttttcaagt ctatcttgcg ttccgtattt aacgtaacta attcttagaa
     1201 caaatgttat cgattttgtt tttcttcttc cgctgttggg atattcaaat cagacacttt
     1261 tatgttcgat tttccagaca tttttttgtt gtttttatta gaaaaagttt gttttttcat
     1321 ttgttttcta aagcatgatc ttcttgtttc ctattttggt cagatatgga tcatgcgtga
     1381 ccacgataac taacgtacct tgggaagcct gttgcgataa gatttcaacg acttgctcct
     1441 tatttcgttt atctaatgat ccagttggtt catcggctaa aatgattttg ctattcttca
     1501 ataagactct cgcacaggcg attctttgct tctcaccacc agataatgtg tagataggcg
     1561 tacttagctt cttgtttata tttacttttt tcaatgcctc atactttgct gttcgcttat
     1621 ctgaaacatt taaattcgaa aacttcaatc caatatctaa attaaaatcg attgtttcat
     1681 attcaatcaa cccaaaatct tgaaaaagaa aagagagggc atttcttttt atcaagcgct
     1741 tctctcttcc agttaaatgt tttgtttctt gattaaataa ataaaattgc cctgaactga
     1801 attgatcatt caatcctaat atattcaata aagtactttt ccctgctccg ctctctccaa
     1861 ctacagtcac aaaatcccca gcatccgcag tgaaacgatc caaatgaaga atcgttttgt
     1921 ttcgaatgat ttttttcgca ccttttatca ctatcaacgg ctccaattgt taacctccat
     1981 tcatcagcaa taactgactt ttcttttctg tagacaaaat aaaaacaact gaaagaataa
     2041 aatcaatgaa gctaaagaaa aaaatattaa aaagatattt aggctctctt tctataagaa
     2101 aaaggaagat tacacttgtc gttccccatt tgatgaggtt catcatgaag aaatctttat
     2161 acgtttcaaa gagcgaataa ccacttgaac gaaagatagc gattttccga caattgtaga
     2221 caaaatatat gcataacatg acatagctca acaagaagaa agaaaagaaa gtgagtaaaa
     2281 atatctgtat caagtaatga aatgatctgc ttaacacttt gacagctatc tcttcagtct
     2341 gagtatacgg aaatgataac tgcaagttat cacgtaactg actttcttca atcagctcaa
     2401 ataacctttc tttcgacaaa gagcctatct tgatctttaa cggtggatag atttctccgc
     2461 tgagaatatt tctttcccaa tacgtactgt ttttcaatgt cagtatttca acaaccggat
     2521 aatgttcgat ccaggggata gaaggaataa atgtataaat gggttgattg ttttttataa
     2581 atagaaattt cggttctttt tcactgattt cttgataata ttctgttatt tgcgcca
//
LOCUS       DASNNU010000166         2627 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.172, whole genome shotgun sequence.
ACCESSION   DASNNU010000166 DASNNU010000000
VERSION     DASNNU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.172"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(839..1357)
                     /locus_tag="U8116_002709"
     CDS             complement(839..1357)
                     /locus_tag="U8116_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEN8435635.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2475..>2627
                     /locus_tag="U8116_002710"
     CDS             2475..>2627
                     /locus_tag="U8116_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN8435636.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
BASE COUNT          868 a          478 c          395 g          886 t
ORIGIN      
        1 gtatttagat aacacgaaag aataaaaata acagctgata gtaaagtttg ttgacgattg
       61 ctactcaatc gaaagtgagt tagtgcgtat gaacttgctt ctgaaaactt atgaaatgaa
      121 agacctttta catcttgcag aaatcgttac tgggattaaa ctaagcaata aaaactaatg
      181 ttcccctcat tctttgaact tctactattt gatttattta ttctgataaa tgacgatttc
      241 accttcagga tcttcaaatc ccgtatagaa tttatttaag atctcttcag tatcagtaat
      301 aggataggcg ataggccaca taccctttga tggttagaat aaactgctca aaatctgtat
      361 cgtatgttgg ctttagcata ttaacaaagg ctatctccaa attataaacc ataagcgttg
      421 aatcgttctt aacaatttca attcttccta acccaaagtc agaagatata attaccggac
      481 ttgccttacc cttcatgtgg gttgttgaga ttccatattt aaagggaatt gtcggtacta
      541 tcggcggtct gaaaaacatc ccaaaaataa aaaaatcttt cccctgctag tctcctaagt
      601 ccctttgtag cggatatggc accataatct cattttatcg aagtgagtca tggtttatct
      661 gttggccaac caaaattgat acaatctaac gatgcaccct ctacggcatt ctaggggagt
      721 gcacttgcat tcacccaata aaaaccggca gttagcaaag aacttatgta gtcgtatgtt
      781 cttactgtta acatacaaga ggtctccttt ccacaattgc ttagcagtaa acgggaattt
      841 acaaagctaa tctcttccat tttcttatct ttgttctaaa agtcccaaat ggagcaactg
      901 tatttacatg aataaattta tatacttccc atacagccgt tttggttgcc tcgtctgccc
      961 attttctttt gtgtgcagtg aatagatctt catcactcat tgaatcaatc atcatgttaa
     1021 tatcatcaat attacgattg agaatttcct ctaattcttt caatggtaga tgtgcatagg
     1081 tatttgtaaa ccacttatat aactctccta actgattcca tttaaattga tcagaaggcg
     1141 ttttaacaga taatccattt ctttcatcat tttcccattt tagaattaaa ttcgtccaac
     1201 caacttgata tgctaaattt tcagcaggtg ttctatccac ttcctcaact cttttatcct
     1261 tcaattcttc aggaatagtt tcaaattcta aaatatattt agtataagtc ctatttattt
     1321 ctttttttaa ttcttcttta tttttatatg ttctcattat attacctcca taaattccta
     1381 attgtctcct ttaattcatc tataatttca tccacgatat tttaatccgt tctacaatgc
     1441 atatctgata tgcattgact gtctaactca tatgtgaatg atttataaaa tatcccaacg
     1501 tctcatctaa agaaatgttg atcgtcagca aggagctaaa aaatacgaat atatttcttt
     1561 tgtagtaaaa tcggactggt ttgcatatca ttttcttcgt aaatatcaaa atcatgttaa
     1621 ctactatcaa ttactaataa aaactaccac tttaagcgtt aagaactgtt aagtttagtt
     1681 aagtgaggat agttcacaaa atccattgtt aaacttcacg aaagtggtaa aactcaaaat
     1741 gctttatata ggattacagc atcgctcttt cttcgattgc gcgttgggta aaaaatatta
     1801 tgaagtaaaa attgacgatg acacgttcct tacggctcat cagatgaaac agctacaaaa
     1861 acgaaatgat caactcgaag aaaatttaat tctaaaaaag cgattgccat attcacgcct
     1921 cgctcgacga actattaaag gtcaagattt acctgaagat catccttttt aggaaattga
     1981 agctttttat gttgcacaat gcatttataa tactacaatg atgttttcac cagatattct
     2041 tctagggtgc ggtgtaatga aacagataca cttgcaaaag aaaattcacc gcgaatttac
     2101 taaaattatg aataattatg ttaatcttcc gaaatttttt tccaaaatta agggatcaag
     2161 ctggtgttat agattgttta aggttggcaa aaaaataact tctttttaaa caattataga
     2221 aaaccgatac tctgttaagt tggttgacac ggtgtcgatt tttatgactt tcttattctg
     2281 aaacttcact aacggacagt acctaaaaag tctatagtat ggacgtttac gagtactatt
     2341 ttatcaaaaa tgctgctcat ttcaacttat atgttttagt gaactacccc cacttagctt
     2401 ggataggcta caattaacta gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac
     2461 caggaggaat ttttatgtct aagagaacac gaagaacttt ttcacaagaa ttcaagcaac
     2521 aaatcgtcaa tctttactta gctggaaagc cacgtgtaga aatcattcga gaatatgaac
     2581 taacggcttc agcatttgac aaatgggtaa agcaatctaa aacgagt
//
LOCUS       DASNNU010000167         2623 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.173, whole genome shotgun sequence.
ACCESSION   DASNNU010000167 DASNNU010000000
VERSION     DASNNU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.173"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..1149
                     /locus_tag="U8116_002711"
                     /pseudo
     CDS             5..1149
                     /locus_tag="U8116_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="U8116_002712"
     CDS             1320..1778
                     /locus_tag="U8116_002712"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEN8435637.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="U8116_002713"
     CDS             1937..2407
                     /locus_tag="U8116_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEN8435638.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(2460..2575)
                     /gene="rrf"
                     /locus_tag="U8116_002714"
     rRNA            complement(2460..2575)
                     /gene="rrf"
                     /locus_tag="U8116_002714"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          860 a          503 c          494 g          766 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaata aaaaaagatg aactatttct agttcatctt tttgcctttg
     2461 cgtggcgacg tcctactctc acaaggggca acccctcact acaatcggcg ctaagaagct
     2521 taacttctgt gttcggcatg gttacaggtg tatccttctc gctatcgcca ccacactgtg
     2581 gtgttatctt ttattgagta aattttgttc actcaaaact gga
//
LOCUS       DASNNU010000168         2523 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.175, whole genome shotgun sequence.
ACCESSION   DASNNU010000168 DASNNU010000000
VERSION     DASNNU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.175"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..633
                     /locus_tag="U8116_002715"
     CDS             22..633
                     /locus_tag="U8116_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN8435639.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            706..1635
                     /locus_tag="U8116_002716"
     CDS             706..1635
                     /locus_tag="U8116_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN8435640.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1704..2523
                     /locus_tag="U8116_002717"
                     /pseudo
     CDS             1704..2523
                     /locus_tag="U8116_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740602.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
BASE COUNT          875 a          357 c          473 g          818 t
ORIGIN      
        1 atattggaag gaggaaaata aatgtatttt atactaccta cactttatag ccttttatgt
       61 gtaatggcac catgtattat ttatatggta tttttttgca aaaaaaatac cctgaaacac
      121 aatatcctga ttttgatttt tatgctgtac tgttggaaag tttttgaatt gactggtaca
      181 ggtggactaa cagatatttt ttatagacct gatacaggta ggaaattttc tctagtacag
      241 ggcagtgtta atcttgtacc attcagtgga ctaaacatta gctttatatt aaatatagta
      301 atgtgtgtgc ccttaggatt cttactacca atcatatgga aaagttatag gaaaatagtt
      361 cctgttttct tggctggggc tctattttca ctaattattg aagtctcaca aattataact
      421 acaagagcaa cagatattga tgatttaata gcaaatacct ttggtgcggt tataggcttt
      481 atactgtggg ctgttttaag tagagtggtg tccttgaagg acaagtcatc tacagtagat
      541 tatttagaac cttatattta tatattgatt ggttttaccg gaaaattctt actgtatttt
      601 cctttctggt ttgcttttga agtattatcc tgataattcc atagaaaata atgagtttga
      661 gttgagaaag aatattcttg tacacaactc tggaaaggag gaagtatgaa aagattctta
      721 aaatgggggc ttgttctttt agcgtctttg attgtgattc taatgtgtac agcggttgtt
      781 atttcttatg tgaatcggcc gtatgcaaga gctgtttatg ataggattaa acgcattctt
      841 gacaaagatt tttatgacca tctatatcta ggttcttcat ttgaaataat aaaccttgat
      901 acaatggata ttcatagcta tatgaaaaaa gaccaagaag aattacccgc ctcattgagc
      961 aaattattta cgattgatta tgcgcttagt gtcctaagtt tagatgaaca gattacaatt
     1021 agtcctactg cacttgacat gcagaaagct gactcatcta ctgcacatct tcaagaagga
     1081 gatattttat ctgtttatga cattatctgt gctatggtct taccctcagg aaatgatgca
     1141 gcttatagtt tggcaattta tacaggtgga aaacttgatc ctgatgcaaa gaatgatgaa
     1201 gaacgctatc aaagcttcat gaaaaactta gatcgttatc taagtgagca aggctataaa
     1261 catactcatt ttactgacgc tgctggctat gactccaatg ctacaacatc agcggaagat
     1321 ttgacaaaag tagctgcaaa gctactaaag aatgataaat ttgcagaaat agttgccaag
     1381 gaaacttata gagcaaaatc aattaaggga aatgtttata actggaatac tacgaatcaa
     1441 tttatgcaac aagataatag atactttact gaaggtgtaa aaggtgtaaa aactggatcg
     1501 tttgatggga gatacaatct atgtgtgtat tatacatcta gttctgataa ccactattta
     1561 cttgtaagtt ttggttcaga gagtgatagt aggcgttatg tagatgttca aaatctattg
     1621 cataatctgc cttgaaattt cgtgccttta agcgcgtatc taaagtttct ttgaaaagtt
     1681 tgagaatgaa gaaagtgagc agtcgaaaaa cggttgctca cttttttgcc ctaaaatcga
     1741 aaaaatataa aaaagtcctt tacaacacgt ctcaggaaaa actgctaagt caattctaat
     1801 atcttgtgga gcaagacttg caccttttga tataagagag cttagagaac taatgagcga
     1861 agatgaacta gaactcgata aaattggaga tagaaaaact gccttgtttg taataatatc
     1921 agatacagat gatacattta attttgtagt ttcaataatg tattctcaat tatttaatct
     1981 actatgtgat aaggcagatg atgtgtatgg tggaagactt ccagttcatg ttaggtgtct
     2041 acttgatgag tttgcaaata tcggtttgat tcctaaattt gaaaaactaa tagcgacaat
     2101 tcgttcaaga gaaatatcgg caagtataat actgcaagca caatctcaat taaaagcaat
     2161 ttataaagac catgcagata caatagttgg taactgtgac tctacacttt ttctaggagg
     2221 aaaagaaaaa acaacagtaa aagaattatc agaaacctta ggaaaagaaa ccatagacct
     2281 atataacaca tcagaaacaa gatcaaatca aaaatctttt ggtctaaatt atcaaaagac
     2341 tggcaaggaa cttatgagtc aagatgaaat aactgtaatg gatggtggta agtgtattta
     2401 ccagctaaga ggagcaagac cttttctatc tgataaattt gatattacaa agcataagaa
     2461 ttacaaattg ttggaagatt atgataagaa aaacttgttt gatgtagaag agtatttaaa
     2521 aca
//
LOCUS       DASNNU010000169         2522 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.176, whole genome shotgun sequence.
ACCESSION   DASNNU010000169 DASNNU010000000
VERSION     DASNNU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.176"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1301
                     /locus_tag="U8116_002718"
     CDS             <1..1301
                     /locus_tag="U8116_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN8435641.1"
                     /translation="LLKFRGEIPLRNLETFQHSAIIISVIFIALNILLGAYVFYNRMI
                     SDIVFVTVIGQVLMTLGIMVVTFAGRWFAFPRLVILLSFVLGTLFLSIYRILVYKLYL
                     RVSRDKKVVIIGLEKDVAPAIQNFGSKKNNKHKVEAVILDNYYANIKNMIKDIDIVYL
                     ASHIEETEKIKIYQLVTKNEKKLFLNTTFENLTLINPNIMNFEDESIIEASGFRIQPE
                     YELFKRLFDIIVSLVLLVIASPFMLFTAILVKVTSPGPIIYKQVRITKNQKEFSIYKF
                     RSMTATAEAKSGPVLAKSNDARVTPVGKFIRAVRFDELPQIFNVLKGDMSIVGPRPER
                     PFFVDQFNAENPYYYLRHNVRAGITGYAQVYGKYVSDYNSKLRFDLLYIKKYSLMLDL
                     KILLQTIKILFDKMSSQGLEEDENVISLAEIAFPNEKIFR"
     gene            1317..2357
                     /locus_tag="U8116_002719"
     CDS             1317..2357
                     /locus_tag="U8116_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN8435642.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2376..>2522
                     /locus_tag="U8116_002720"
     CDS             2376..>2522
                     /locus_tag="U8116_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN8435643.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYH"
BASE COUNT          870 a          403 c          467 g          782 t
ORIGIN      
        1 tcttactgaa gtttcgtgga gagatcccat tacgaaattt agaaactttc caacactcag
       61 caattattat ttcagtgatt tttattgcgt tgaacatttt gttgggtgca tatgtttttt
      121 ataatcgaat gatcagtgac atcgtgtttg tcacagtcat cggtcaggta ttgatgacac
      181 tagggatcat ggtagtgact tttgcgggcc gctggtttgc ttttccacgt cttgttatct
      241 tactttcatt tgttctcggt acgcttttct taagtatcta tcgtattctc gtatataaac
      301 tttatttacg cgtaagtcga gataaaaagg tcgtcattat tgggttagaa aaggatgtcg
      361 caccagcaat ccaaaatttt ggttcaaaga aaaacaacaa acataaagtc gaagcagtca
      421 ttcttgataa ctattatgca aacatcaaaa atatgattaa agacatcgat attgtctatc
      481 ttgcttctca tatcgaagaa actgaaaaaa tcaaaatcta tcaattagtt actaaaaatg
      541 aaaaaaaatt gtttttgaat acaacatttg aaaacttgac attgatcaat ccaaatatta
      601 tgaatttcga agatgaaagt attattgaag catctggatt tagaatccaa cctgaatatg
      661 aactattcaa acgtttgttt gatatcatcg tttcacttgt tttattagtt attgcttcac
      721 catttatgtt attcacagca attttagtca aagtgacatc accgggacca atcatctata
      781 aacaagtgcg gatcacaaaa aatcaaaagg aattttcaat ctataaattc cgctcgatga
      841 ccgccacagc cgaagcaaaa tctggaccag ttttagctaa gagcaatgat gcacgagtaa
      901 cgcctgtagg taaatttatt cgagctgtcc gctttgatga attaccacaa atcttcaatg
      961 tgctgaaagg cgatatgtcg atcgtaggac cacgtcctga acgtcctttc ttcgtcgatc
     1021 agttcaatgc agaaaaccct tattactact tacggcataa tgttcgtgct ggtatcactg
     1081 ggtatgcaca agtctatgga aaatatgtct ctgattacaa cagtaaatta cgttttgatt
     1141 tactttatat caaaaaatat tcattgatgc ttgatttaaa gatcttgctg caaaccatca
     1201 agatcctatt tgataaaatg tcctctcaag gacttgaaga agacgaaaat gtgatctcat
     1261 tggcagaaat tgcttttccg aatgaaaaaa tctttcgcta gttgggaggc gtaaaaatga
     1321 acaatccgaa attcagcata atcgtaccag tatacaatgc agaagcaact attggtcgaa
     1381 cgattgatat tctaaaagaa ttggattatg aagagtacga agtcgtttta gtaaatgatg
     1441 gatcaacaga tcaaacacag gaaatcattc aagaactgat tgaaaatgat gaacgttttg
     1501 aattgattac tacgaccaat caagggcccg gattagcaag aaacgaagga ataagacagt
     1561 caaaaggaga atatctctta ttcttcgatg cagatgacac gccagtaaaa acgatcttgt
     1621 cagattatga tcatttgcta gaaaagagtc caaaagcgga tcttgttgtt agttcgtttg
     1681 tttttcgtac aatggacgag ggaagaatcg tttccgaaaa agaaaactta gttacagaat
     1741 gtcactatct aactcacgaa gagtttataa aagacatgta tcagttaatg aatgaacagt
     1801 tgatgtatgt tgtatggaac aaatgctatc gtagcgatat tctgaaaaat aaccaaattc
     1861 tatttaaaac ttataatagt tgtgaggatc gaatatttaa cttacattat tatgaatact
     1921 gtcatcaagt gatcatgaac ccaaaagtag aatatattta tgaatttgaa ggtgggaagg
     1981 ggatcactaa tcaataccga cctaacaagt tcgaaacatt taaagaattc tattcattag
     2041 caaatactgt aacagacgat attaataaag acggtatggc ggcgcttctt ttaaaaggga
     2101 caacttcagt tattttttct atttatagta cctctgcaag aacttcaaaa gagaagaaag
     2161 acgaagcaaa gcagatattg agtgatccag tgatccgaga agccaagcaa attgctaaga
     2221 cggattcttc agcaaaaaaa ataacaaagt tgctttacaa tatgccagct ccactatttt
     2281 tatctgctgt aaaagttgga agcttcgttg aagtgaaaat gccagggtta atggctgctt
     2341 tgaagcgagt atattagtta atggaggaaa atataatgaa aagaattttg cattttcagg
     2401 gacgaatggg tttaggtgga gcagagtctt tcatgatgaa tttatatcgt aaaattgatc
     2461 gtacaaacta tcaatttgat tttttgattt atgaagatta tgcagatgtt caagactatc
     2521 at
//
LOCUS       DASNNU010000170         2494 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.177, whole genome shotgun sequence.
ACCESSION   DASNNU010000170 DASNNU010000000
VERSION     DASNNU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.177"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(5..280)
                     /locus_tag="U8116_002721"
     CDS             complement(5..280)
                     /locus_tag="U8116_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8435644.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            512..1246
                     /locus_tag="U8116_002722"
     CDS             512..1246
                     /locus_tag="U8116_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8435645.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            1285..2427
                     /locus_tag="U8116_002723"
     CDS             1285..2427
                     /locus_tag="U8116_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN8435646.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT          960 a          404 c          411 g          719 t
ORIGIN      
        1 ttactcaacc tttataccgt tccatgaaac tatcaatatc agatttttta aatctaatga
       61 ccgtatcaat ttttataaca ggtaacccgt attgagaaac atattttcta atcgttccta
      121 ctgatacacc tagatattca cttgcctgct gatagtccat atactctaga gtgactccag
      181 aaataatgtc ttctagcata ggctttagca ctgtaattat ctctttcact tgttgttcaa
      241 cttgtataga aacaatgcta tcaatgattt gtagagacat atttccccct cctttcgtga
      301 ttgcttgaac aaattttcta agaattatct tcgtattttt tgctcaaaat aaaagtattt
      361 tgttattaac cgattaatca aacaaaaaat gattaccagt tcatcgacat acacataata
      421 cctcgataaa aaagaggtgt caatatcttt gtaaaattta tttacaacac ccctttttcg
      481 aggtatacta attttaggag gtgtggtcaa tatgataaaa cgtccaccaa taaattattt
      541 agaacgaaaa aagatactcg gaacaaaaat aaaagctata agaaagagta aaaaattaac
      601 acaaccagca tttggtctta tgattaacaa cggtcaatta attgacaaaa aaactatcta
      661 cgaatgggag aagggaacct atttaccaat accagagcga ttatcccgaa ttgctgattt
      721 gggaaatatg agtatagagg agttagtttg tggcaatgta gaagaatata ttttgggaat
      781 tattctttat agagatagca ttgtactaga tggtataaca tttccagata aaaacctgtt
      841 ccagcatctt cgtcaacagt tcccaccagt acactctaat ttagatacat ggctggatag
      901 atattccaaa ttagaaccag agatgcaaga atttattgct aacaagactt gtaataaagt
      961 taaaaatgaa aaaattagtc ttttcaatat cttaaaaata gaagaattat tcatcaatgc
     1021 tattgtagaa gaatttgaca ataacattct tttcctcact agcagtatag aagaactact
     1081 agaaagaatg gtagatgaat ggctacctat tcaattgaaa gacatgtctt atcccgaaga
     1141 agctgtgaga gaaattactg ataatataaa caagctagaa caaacgattt catcaattgg
     1201 gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc atctagtggt aagtactcct
     1261 tgtaaactaa gaggagatga tttcatggca aaaatatcaa agtacaaaaa gaaaaacggt
     1321 caagtggcgt ggatgtataa aggacacatt gccaccgatc cacgaactgg tgaaaaaata
     1381 aatactacga gaagaggttt tagtagcaaa caagaagctc aaaaagatta tgacgagtac
     1441 agacatcaaa tattatacgg tgttaaaaag aaagcacctg atatgacatt tgaagacctg
     1501 tataatgaat ggataactca tcagcgtacc agtgttaaag cttcaacaat cgcaatctca
     1561 gtaagatacg caaataatca aatacttcca gcatttggta agcttaaact atcgaatatc
     1621 tctgtcccat attgtcaaaa agtggtagat gaatggcaca gtaaatatga aagttacgat
     1681 tatatgagaa aacagactgc acaaatttta cgctacgggg ttgcaatgca atatattgac
     1741 aataatccta tggaaaaaac acttcttcca cgaaaaaaag agtatgagaa aaatcgtaaa
     1801 ttttactcaa aagaggagct taataattta ctggatgctt ttaaagattt tggtaacatg
     1861 aaacaatacg ccttcttcag attattggca tacacaggta tgagaaagag tgaagtactc
     1921 gctttacagt ggaaagacat agacacgttt aataaagaac tacatgtcaa taaaactctt
     1981 gctgtagatg aatttggtaa agttataatt caatctccga aaacaagagc ttctagacga
     2041 gttatctctt tagatactga gactctttct atcctcaaca actggaaatt acagcaaaaa
     2101 gaagagtatt taaaacttgg atacaatact agctctaaag agcaacatgt ttttacaaca
     2161 gttaaaaaca ctctatacat tccaaacact gttaatgatt ggttaagata tattttgaaa
     2221 aaatataact tgcctcgtat tacacctcat gggtttagac atacacatgc tagtttatta
     2281 ttagaagcag gagaatctgt caaggtagtt caacaacgat tagggcatga aaatagtaaa
     2341 gtgacattag acatctatgc acatattaca aataatgcac ctaagaaaac aggtcaagac
     2401 tttgctgata tgatggctca tcagtaaaag agctggtcaa aatctggtca tttctagtat
     2461 acaggcaaaa caaaaaagct agaaccctta tata
//
LOCUS       DASNNU010000171         2459 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.178, whole genome shotgun sequence.
ACCESSION   DASNNU010000171 DASNNU010000000
VERSION     DASNNU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.178"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1155)
                     /locus_tag="U8116_002724"
     CDS             complement(<1..1155)
                     /locus_tag="U8116_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN8435647.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSV"
     gene            complement(1208..1879)
                     /locus_tag="U8116_002725"
     CDS             complement(1208..1879)
                     /locus_tag="U8116_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN8435648.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(1939..2148)
                     /locus_tag="U8116_002726"
     CDS             complement(1939..2148)
                     /locus_tag="U8116_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435649.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(2215..2454)
                     /locus_tag="U8116_002727"
     CDS             complement(2215..2454)
                     /locus_tag="U8116_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435650.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT          644 a          551 c          363 g          901 t
ORIGIN      
        1 tacactgtaa ggagaagttt cgcttttcac accgtttact gtgataccgt ctgcgccacc
       61 tgcaatgtct gttggaatat gaatcgttcc ttgataattt actgcgtcac caatttggaa
      121 agaatgttct tgtcctgttc catttgttac tgttttatcg atcactggtg ctttatagtt
      181 tacgtatgaa gcttttaagt cacttgtcac gccatcattc gtaatttcaa aggttaattt
      241 attgtgcgca tcgttgcgag cgtctgcgcc taataaatat tgatctgctg ttggttcttg
      301 atctgatccc actacaactt ccgaaggaac ttccacaaaa tagtattttc ctaacgtcaa
      361 atcagtcgca gtcagctttc cttgatcatc cgatacaaga tcacccaatt ttgttccttt
      421 tccaggttcg cccttgtata actcaaattt tgcccctttt aaaagggttt gtttgctttg
      481 gtctgcgcca tcgtcaccaa atttcgttaa ttcaaaactc aacttagaaa caatgttctt
      541 aggatacaaa tgaatatttt ttaaaaatcc tgatcctgtt ttatctgtca ttggcgcaat
      601 cacaaccatt ggttttgctt tttgtgtgac taatccagca gcggatttac tttcataaac
      661 cacatataca ttccctttgg aattgacata ggctggttga tcagtaaaag tgatctcacc
      721 attttcatct acagctgaag taattttgtt ggttgcattc ttgacgtact cactgttaga
      781 tcccttgtct tctacatctt gtacaatctc gtccactttc gcgttggtta gatcagaatc
      841 ttctctcggt aatacttgat ccgtaatatt tcctaacgta aactcaacat caccataatc
      901 tgccttgttg tatttcgtta cacctgtagg atattgatcg tgcgtataac catcgttttt
      961 gatcccgtca atatcaacat tcaattgcgt tccttgatcg tacattaatt tatgaaccgt
     1021 gatattgacc ttctctggtg gtgttttttg cgtgacatct tctgcatgta cttgataccc
     1081 ctgaaagcag cctgcaatga ttcctaacat tccaacaagc attccagctt tttttacata
     1141 ccgatttatt gccatttttt actctcctca tttcatattt caacgactcg cgttgattaa
     1201 aaccttgtta ttgtagctct ccctgtacca cgtatctttc ttttccttgt gacgtataaa
     1261 cacaagtaaa caacgttaat aagggtttct ctcctttctt tggcaaagac aagaaagagt
     1321 tctcttcagg aacgccatcg attaatttaa agtacccagg tacgataaat ttacgcgtta
     1381 ccttgtaacg atagacgcca tcgttactaa ctaagtccat cacctcccct aatgacaact
     1441 tttgaatatc agaaaataaa acacctggca tttccatgtt atgacctgct aggacatagt
     1501 ttcctttgcc catttcggca tcttcataaa aataagtagc cgctccaaga ctcaaagtgt
     1561 attgatttgc ccctttaaaa attggcaatg ctaaaccaag tgcaggaatc tgaatttgtg
     1621 caaatacttg tttattgacg cccatttcga ttgcttcttt ttgtatggac aacatttgcc
     1681 ccaaagacat atcattattg acgttcgttg catcctcctt ataactcttt gcatgtgcat
     1741 tgatttgatc tacgggcatc tttttttcta tattttgctt aaacacctct ccttcatgtt
     1801 tttggtagct aacatagcca aacacactgc aaaagacgat taaagtgact cctaaaaaac
     1861 caaataacca ttttttcatc catctgcctc ctttcagaca aaacaaaaaa actttttctc
     1921 atttccatag aaaaagtttt aaaaatgaat tctataagtt cgtctcaact gttcaaagtt
     1981 tttcattgaa cctaacaaac aagctttctc ttttcctgtt aactgcatat cattgatata
     2041 ttgcgcgaca gataatcttt taaggtgttt ttgcttcatc gtttttagta acttttcttc
     2101 catttctttc gacacttttt ttctgttttc gatttcttca taattcatga gttttcctac
     2161 cttccttgtt ttttgcaaaa ataaaaaagc ctatgatcac atacgctcta cacttcagtc
     2221 ttgggttatc tttttctttc gtttaattac tggccgtgct ggtttcaaat ccttgattcc
     2281 attatgggtt ctccaataat cgaattcttt tcgcaatctt tctttttcca tatctgttcc
     2341 tttagataag gctttgaata cttctttagg tgaataacca cgagctaaca gctgtacata
     2401 ccttcctttt gtcaaagtat ccatatccat cgcctctaaa actggtcgcg ccatctact
//
LOCUS       DASNNU010000172         2420 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.179, whole genome shotgun sequence.
ACCESSION   DASNNU010000172 DASNNU010000000
VERSION     DASNNU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.179"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            94..>2420
                     /gene="esp"
                     /locus_tag="U8116_002728"
     CDS             94..>2420
                     /gene="esp"
                     /locus_tag="U8116_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEN8435651.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          875 a          378 c          492 g          675 t
ORIGIN      
        1 ggttagtaag attcctattc tgtgaataat acgtatcaga tttatctttt attcttacta
       61 ggaaagtaat tattaaggga gttgatttga ataatggtta gcaagaataa taagagagta
      121 tttctagaaa aaacaaagaa acgagtattg aagtacagta tcaaaaaatt aagtgttggg
      181 gttgcatctg ttttgattgg tgttggtctt gtcttgggaa caactgaact agttaaagca
      241 caagatgaaa tttctccaag cagcccactt gaaacagcga taagctccgt acaagtaggt
      301 gacaaagtag cttcggggaa tacttttcag gagaatcctg gttatacgaa aaattataac
      361 ttttcggatt tacaatttag tcctcaagaa ctaactggag acacactaaa aggcaataca
      421 attggatttg aagtttacgg aaaacataat attgcagcat caactaaaaa ctgggaaatc
      481 cgtcttcaat tagatgaacg attagctaaa tatgtagaaa aaattcaggt cgatccaaag
      541 aaaggaattg gatcttctag acggaccttt gtaagaatta atgattcgct gggtagacct
      601 acaaatattt ggaaggtcaa ttatattcgt gcaagtgatg gattatttgc tggggcagaa
      661 acaaccgata cgcaaaccgc tcctaatggg gtaattacgt ttgaaaagag cctagatgaa
      721 atttttaaag agataggcat agacaatctc aagaccgatc gtttaatgta tcgaatctac
      781 ttggtaagtc atcaagacga tgataaaatt gtaccgggta ttgatagtac gggctacttt
      841 ttaacagatt cagatgattt ctataatagc ttagatgtat ctgaaaataa tcctgaccag
      901 tttaaacatg gatcagtaag tgcaaaatat gagaaaccta atactcaaac aaaagatggt
      961 tcaggatcca caggtgctaa tggagctata atcttagatc ataagttaac gaaaaattat
     1021 aatttctcat attcatcttc cgcaaagggc acgccttggt atgctaatta caaaattgat
     1081 gaacgattag ttccatatgt agcgggtata cagatgcaca tggttcaggc tgacaaagta
     1141 acatatgatg tttcgtttga atccggaaca aaagtagctg atttagcaat cgagcgacgg
     1201 gaaggtcatg aaaattatgg cataggttca ataactaata atgatttaac taaacttatc
     1261 gattttgcta atgcaagtcc acgtccagtc gttattcgat atgttttaca attgactaag
     1321 ccattagatg aaatcttaga agatatgaaa gcaacagcac aagttgaaga aaataaacca
     1381 tttggtgaag atttcatctt tgattcttgg ttgtcggata cgaataaaaa attaatccag
     1441 aacacttatg gaacaggtta ttattatttg caagatattg atggtgatgg aaaccctgac
     1501 gataaagaag agagcggaga cacgaatcca tatatcggga aacctgaatt agaagaagta
     1561 tatgatgttg acacaacagt taaggggaaa gtattcatcc acgagttagc gggaacaggt
     1621 cacaaagccc aacttgttga taaagaaggt actgtattag cagaaaaaac tatcgctcca
     1681 aatgaaaaag atggggctcc aatttcagat actgtagaat ttgaatttac gggtgtagat
     1741 tcaagtaaat taatcgcgaa agatgaatta aaaatccaaa tcgtttctcc aggttttgat
     1801 aaaccagaag aaggttcaac cgttattaag gaatcaccaa aagcggttga taaacaaacc
     1861 gtggtagttg gatttaaacc agatgctaaa gaatcaattc ggaataataa aaacttacct
     1921 gaagatgcag agtattcatg gaaaacagag cctgatactt ctaacgttac tgatagtacg
     1981 aaaggtattg taactgttaa gatcggaaat cgaactttcg acgtggatgt agagtttgct
     2041 gtaaaagctt ctcaagctat ggaaaatgat gcaacatacg tacctataac aacaactcca
     2101 gaaacgacag ttcaaagtgg aaaacctaca tttgataaac cagatgttcc tttagctaaa
     2161 gatgcctttt caatttcaga tgtctataat aagtacttcg gtaatgcaag tgttgatgca
     2221 aacactggta ttgttacatt cactccagct aaaggtgtag gagaatcgga gccgattact
     2281 ggaacaattc ctattaaaat tgtttaccaa gatggttctg taggcacgac cgatttagca
     2341 gtaactgtaa gtaaagatat ttatgaaaat ccaggagaaa acattcctgc aggctaccac
     2401 aaagtaacct tcaccgcagg
//
LOCUS       DASNNU010000173         2399 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.180, whole genome shotgun sequence.
ACCESSION   DASNNU010000173 DASNNU010000000
VERSION     DASNNU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.180"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            207..2069
                     /locus_tag="U8116_002729"
     CDS             207..2069
                     /locus_tag="U8116_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN8435652.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2399
                     /locus_tag="U8116_002730"
     CDS             2069..>2399
                     /locus_tag="U8116_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN8435653.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatt
//
LOCUS       DASNNU010000174         2370 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.181, whole genome shotgun sequence.
ACCESSION   DASNNU010000174 DASNNU010000000
VERSION     DASNNU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.181"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            178..564
                     /locus_tag="U8116_002731"
     CDS             178..564
                     /locus_tag="U8116_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN8435654.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            658..849
                     /locus_tag="U8116_002732"
     CDS             658..849
                     /locus_tag="U8116_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8435655.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1363..1695
                     /locus_tag="U8116_002733"
     CDS             1363..1695
                     /locus_tag="U8116_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN8435656.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1991..2146
                     /locus_tag="U8116_002734"
     CDS             1991..2146
                     /locus_tag="U8116_002734"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN8435657.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT          855 a          395 c          395 g          725 t
ORIGIN      
        1 gtataaagat aactatccat tatcaactaa aaaaacattt ttgaaaataa acatcagcag
       61 tcctatcaat gcatgtgagc ttatagaagt tattcgcagc gcagcataat tgcattagct
      121 tcttttcgtg ttttttgcct cttttccctt taaatcttaa tggaggaggt gtgcaatatg
      181 tcagataacg acgaacgcgt aaatgcccag ttgattcgat tctttgaaaa ggtcattttg
      241 aacaccgcta atacttatta taaaaaacaa gaagaaattc aaaaacatga acaacttgat
      301 caactcccac catatttgtt tactaaagat actataaaat ttaaacaacc gattaatgtt
      361 ttaaatgtga ctctccttgt tgaagatata aaattagcaa aaatccttcc tttgttgaaa
      421 gaacctgaac aaatttttct tatcgaaaaa tttatctttg ataaaactga taaagaaatt
      481 ggaaaggttc tggggattac aagacagggt gcgacaaatt taaaacatcg actttacaaa
      541 aaaattcaaa aaatgctggt ttaatatctg cataataact aattttgtat gcttctttgc
      601 tcgttctcct ccttcccaaa gaaatagatt ataagtccat gggaagaagg tagatttatg
      661 aattctttaa aagaagattt cattttagca aaggctggaa atgaagaagc tgttgaggca
      721 attctcaaaa gatttagctc tttgattcat aagcaatcct ggagaactgg aaaatatgat
      781 caagattgtt atcaagaatg tatgcttgca atatatctag ctatctcgaa attcgaaatt
      841 aaagaataac tcaactaagg gagtctgtga cataagtaaa aagatagttt caaatttcga
      901 acaataaatt tgaaactatc ttctttataa ttataaagta tcgattttac agcgttctat
      961 aatgattcat agaatctaaa acgaatgatt ttaattatca acgtttttta attcgttttt
     1021 agcttaaaaa taacttttgt cacagtctca ctcctaaaat tactttattt acgtatgaag
     1081 tgggcaggag gtcgaatcca gaactgggct atggtacttc aacgaggact tctcctctag
     1141 aattgaaaag catgcgattt accttccgta acgacggtag atttattcaa ctatgtgtcc
     1201 aatcaaaatt tgtctgattt caaaaatagt caagagaatt ttggttgtta aaaactctta
     1261 tctatcaact tccacagttt agttgacaaa ccctgatact aagttgggct atgatacact
     1321 tttcttgact atcagttacg ttaccggaag gaatgaactc tattgagaaa aacatatgat
     1381 cgtaaattta aactaaagat ttcacaggat atccttgaga aaaaagtaac taccaagaaa
     1441 attgctgaag aatacaatat ctctcgtcca acaatttccc gatgggtttc agaatatcgt
     1501 cgatacggga aaaatgcttt tgctggacaa ggaaaaagat tacctgataa agcagatttc
     1561 tacattcttg aacaggaaaa taaacgacta aaagaggaag acgagtgttc tacgaacaca
     1621 aagatcgata cggatctcca gggatcactc aaaaacttta cgatgaagga atcgaaatca
     1681 ataaacgtgt tgtagctgtt cttatgcgtg aaatgaattt atgcacaaga ggatttcatc
     1741 gacgcaaatc ttctcatggt agagaaaaaa caatcaaaga aatggtaaaa aaatgttgaa
     1801 tcgataattt gaacagaacc aaattgatgc tgtttgggtg atggatatta cttacattcc
     1861 atgtaatgac ggtcgcctct atttgtccac ctatattgac ttagcaactc gtattctacg
     1921 ttactatatg gtagattcac acatgaaaaa agagattgtc gtccgtccat tacaaattta
     1981 ttaaggagaa ttgcctacgg taattcattc cgatagaggt agccagcatc ggtcttttat
     2041 ttatcaagag ctgttgaacg accaccacat cacacattca gtgagccaaa ctgggactcc
     2101 tgttgacaat gcagtaattg aagcgtttca tcgatcaatc aaatgagaat taatcgactc
     2161 caacaaatat aaaagtaaaa tagagatgaa ggtgttaatc caagattatt tagaagacta
     2221 ctacccaaac aaaagaatcc atacaaaatt tatgatgact ccttgacaat ttgaagcgaa
     2281 gaaaataagg gatctataat atctagtgtt ggtgacgcct tctgttgcca atactttttc
     2341 ttttttctaa taaacgcaaa cagacacctt
//
LOCUS       DASNNU010000175         2349 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.182, whole genome shotgun sequence.
ACCESSION   DASNNU010000175 DASNNU010000000
VERSION     DASNNU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.182"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            239..>2349
                     /gene="acm"
                     /locus_tag="U8116_002735"
     CDS             239..>2349
                     /gene="acm"
                     /locus_tag="U8116_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEN8435658.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIG"
BASE COUNT          934 a          352 c          454 g          609 t
ORIGIN      
        1 tgaaaaaatg aaagcaataa gtaaaataaa atgaatatat gcgaaaaaat aatttacatt
       61 aggaaaaacc ttgtaaaaac agttttttgg catatttatt ttttataaat atttgtaaaa
      121 taactatcaa ttttttagtt aaaagattgg aaatgagtgg gggaggaatt gaagaaagga
      181 tttttgagag atgatatagt aggttttatt tttagttaaa ataggggaga tagaaaaaat
      241 gaaaaagtgt agaaatatta ttttatcaat gttatttatt attactaata taacatcact
      301 gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg tcacttcgtt
      361 gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat tctcgtttga
      421 cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc caagtgaagg
      481 aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa ataaaaatgt
      541 tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa atattaaaaa
      601 cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg taaccgatac
      661 cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag ctactgtaaa
      721 tgtgacaaag ccagcttctg gttcttcttc tagcgttttt tattataaaa caggagacat
      781 gctacctgaa gatactaagc acattcgatg gtttttgaat attaacaata acggcactta
      841 tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt ggacaaagat tagatccaag
      901 tacgtttgaa attaatcaaa tacacttagg tgaacagaag gtttatagag gagaagaggg
      961 aatccaacaa tttttacaag atttccccag cgctacattt aatttttcag taactgataa
     1021 ttacattgaa ataactatac caaaaaactt tgtcaatctt aggaaaataa tggtgagtta
     1081 taagacgatc atagaaaacc cagaacaaat aaattttgag aatcattcgg aagcttggtt
     1141 taaagaattt aataaacctg ctgttgatgg agaaagtttc aatcatacag ttaaaaatat
     1201 ttcagcttct ggtggagtca acggtacagt tagaggcgaa ttgaagattt tcaaatatat
     1261 caatgacaca gagattggta tcccaaatgt gactttcgaa ttaagacgag cagatgaaca
     1321 gcctattcaa ggacagtcta gtatcctgtt gacttcgaat gaacagggag aagctagtat
     1381 caaaggcctg caagtaggag actatgttgt aaaagaaaaa gaagcaccca attggattga
     1441 ttttgatcca ctaagttcaa atgaactcaa attctccata aacgaaaatg atacagaggg
     1501 agtcagttta cctatttata acaagaaaaa ggttacgaac atcacagcaa ctaaaatttg
     1561 gaacggtgga accactccaa gaccgagtat ttattttaaa ttattcagag caagtacaaa
     1621 caattgggaa ccagttcctg atgcagaaac aaaacgatta gacaatggaa taacttcagt
     1681 gacttgggaa gatatccaac agtatgatga tagtggaaac gaatatacgt ttaaagtgca
     1741 agaagtagat caaaacggaa atgattatgt cccttcaggt tatcaaaaaa ttgaaaacgg
     1801 gctagtcgtt acaaatgaga ataaagaagt catttcagtc tcaggacaaa aaacatggga
     1861 agacaatgag aaccaagacg gcaaacgacc gacgacgatc acagtaaatc tattagccga
     1921 cggtcaaccg atccaacaca aagaagtaag tgaaaaagat gattggcgct atcgtttcac
     1981 aaatctgcca aaatacagag acgggcaaga aatcatctat actgtaacgg aagacaacgt
     2041 gcccgaatac agtacgacga ttgacgggta taacatcaaa aacagttata caccggaaaa
     2101 aacaaatata tctataccag gtagccatat aacaccgtgg aaaaattttc cagagaagac
     2161 agagagagag aaaattagaa atctattctc tcaaaaattg caaatatttg ctcatacagg
     2221 agaatccagc gaacaaagaa gcaattatat tagtaagaga gctgtaccta aacagataac
     2281 aaataaatca aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt ttgctgtaat
     2341 cattggttt
//
LOCUS       DASNNU010000176         2281 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.183, whole genome shotgun sequence.
ACCESSION   DASNNU010000176 DASNNU010000000
VERSION     DASNNU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.183"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(149..448)
                     /locus_tag="U8116_002736"
     CDS             complement(149..448)
                     /locus_tag="U8116_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN8435659.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            799..1431
                     /locus_tag="U8116_002737"
     CDS             799..1431
                     /locus_tag="U8116_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN8435660.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1554..2192
                     /locus_tag="U8116_002738"
     CDS             1554..2192
                     /locus_tag="U8116_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN8435661.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          765 a          362 c          332 g          822 t
ORIGIN      
        1 tgttaattaa aggataaaat acaatataaa gaaacatctg tttctttatt tagaataaaa
       61 aaatacagac tctgttcaaa taaatgaacc tagcctgcat tatgagattg actatacttt
      121 ttaattcatt gagatgaata actcgttttt ataaatgccc aaaagtaatc ggtgccgaag
      181 ccattcctcg acctaattct ttttcaaatt ttcctattgt tgatatctcc atataaaacg
      241 gtgatactgg tttagacaat tctcccctga ttgctaacgg acgtaaacta cgttccaaat
      301 tatttatgac tttaggataa tactgacctt tttctaagcc tgtctttgcg tctatcaaaa
      361 gtatacgttc cttttctttg atctctggat ttaaaacgag atcatataat tttagtacca
      421 tgttttctat ttcttcactt ttagacatca cgatttcctc ccaaaaatat atttcttccc
      481 actacattct ataaacaaag tatacgctac atctagattt ttttaaatca ttactggatt
      541 gttaaaacct ttcaatacac tgttatggca acgattttta ttttaaaaac attctaaatt
      601 aaatcagtaa aaatttttat tctaagattt ttattagaat tacgcctaat ttgtgtctct
      661 cagggcaatt ctaagaacgt ttcaagagat agcgacacta tcgttatcga aaaaggcgaa
      721 aacctatctc attttctgct aatgtaagtt ggtttagctt ttgttaatat gactttatga
      781 aaatggaggt aattaaatat gatttttcct aaagttaatg aagcaagctt gaataaagga
      841 tccttttttc atatctatga aaacgaagat tttgaactgt ataatatcct ttcacaaaaa
      901 gaggtcatcg aaaaccaaat aatctgccat ccaattattt acaaatctat tttcaagtat
      961 gcattttttg cttccaatga ttttcaaaga atggagatta ccgactgtat attcgaaaac
     1021 tgtgattttt caggctgtat attgacaaaa agcatgttgc atcgcgtgaa atttgtcaat
     1081 tgtaaattaa caggtactaa attgatggaa agttatattg ggaacacgtt attcgagaat
     1141 tgtaaaatgg actatgtaaa tttatctggt tcaaacataa aagaaagtac ctttgagcac
     1201 tctgtcttat caagcgcaga ttttgtagac tgttccctca ctaaaataat gttttctact
     1261 aatgatttag aaaatgctag cttttttgat actgatttaa aaaatatcga tttaagtagc
     1321 aactcctttg aaatgattga agtacgctcg gaacatctaa aaaattgtac gcttaatcag
     1381 ttgcaagcac ttggttttgc tagaaaattt ttacaaataa aagtagtata atcaaaatga
     1441 atatataaga taaattttta gcaatacaaa tatccataaa aaaacattgc ctattttaac
     1501 tcttcatgta tactgactct atcttcaaaa actgaatagt aggagttatt atcatgaata
     1561 acattttagg aaaaaaaata gaacttaaaa aagggatatt gggcactatt gtcgttgtac
     1621 tcggtttgtt agggttgcta tttgcgccat ccattattag cattttttct ttaactatca
     1681 ctggatttat agcatttaca cttgtttttg gtaaaaaaga tacgaaaaaa atgttctcga
     1741 aacctgtcgc tcctgtcaaa aatatagcca aatatttttt tatcaatgta gtaatcagtg
     1801 ccgcagtctc ggtatttttg caacaaattt tgaaatgggg tttaacaggc aatccaataa
     1861 atgaggcttt cagcccgtta ttatttatta ttttgcctat catgatactt ggagaagaat
     1921 tgttctcagt gtattttctc gctatttttt ctagtaagtt cagcattcct gtcgctagtt
     1981 tcttgtcagc aatcatcttt ggatttatcc attacagtac ttatgataat ggaaatattt
     2041 tacacactgt agcacacatc cttttgatac aaggggtggc aagattgtta ttcaaccaag
     2101 ctgccattaa aagcaactcg atcattacaa gttgggcagt tcatgtgata tttgatttca
     2161 cagctatatt gctagtcgtt ttattctcct aaatttttat agaaataata catgctctat
     2221 tgctaagaaa gcatacttta tttcctttaa acaaaaagac agatgtatca atggattaaa
     2281 a
//
LOCUS       DASNNU010000177         2259 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.184, whole genome shotgun sequence.
ACCESSION   DASNNU010000177 DASNNU010000000
VERSION     DASNNU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.184"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(148..2145)
                     /locus_tag="U8116_002739"
     CDS             complement(148..2145)
                     /locus_tag="U8116_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEN8435662.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          574 a          505 c          411 g          769 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaa
//
LOCUS       DASNNU010000178         2238 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.185, whole genome shotgun sequence.
ACCESSION   DASNNU010000178 DASNNU010000000
VERSION     DASNNU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.185"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            162..272
                     /locus_tag="U8116_002740"
                     /pseudo
     CDS             162..272
                     /locus_tag="U8116_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(369..845)
                     /locus_tag="U8116_002741"
     CDS             complement(369..845)
                     /locus_tag="U8116_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN8435663.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(858..>2238)
                     /locus_tag="U8116_002742"
     CDS             complement(858..>2238)
                     /locus_tag="U8116_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN8435664.1"
                     /translation="PFLDQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKG
                     TAGLAGGVAYLVYTATISGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELP
                     QFLGFFGGSRFIPIVSSLAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGF
                     LLRLTGAVGLHHTIYPLFWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGT
                     RFFAGRFATMMFGLPAACYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFL
                     FVAPWLYVIHAFLDGLSFYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIP
                     FGIAWAFIYFFVFTFFIKKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGG
                     KENIESVEACATRLRVAVTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQE
                     INQILGKDN"
BASE COUNT          792 a          424 c          309 g          713 t
ORIGIN      
        1 acatctttca gcattttact atatttggta tatagtgcta aggttcatat tcgattgaca
       61 agagaatata aattgatcta tttctatttc ctaagttgag taaacataac tttgcccttt
      121 ataaatatct tccctctctc tcttctctct atattttata tttgaataat catatcaagg
      181 tattccaacg aaatgccaat ggatttcgaa gcttttataa ctttaaatta cggatcatga
      241 tttggcatgg ggaatcatta ctaataaaat aaaaaaatga acagaaagaa aacagttctt
      301 tctgttcacc aacttcattt gacaaatatc ctatttagct tattttatag ctttttatat
      361 acactttttt aaaaagtgat ttttccacat gaattcccat gagcaattcg accattatct
      421 attattaaat cattgaactg atttttctcc ggaaaaacaa ctataacaga agtatcttta
      481 ccagatttta ttattttgtc aaaatccaca tcaaccaacg aggtttcagg agtaacctca
      541 tcattttctt gaacacaaat cgaaaaaggc tctccttgta attcaactgt atcaattcca
      601 atatgaatca aataatcaat gcctgagttg ctttttatat taatagcatg tttagtagga
      661 aatatatttg ttactcgacc agccaatgga gcataaattt ttgaaccatc aggtatgacg
      721 gcaaaaccat cacctagtaa tttttgtgaa aagacaggat catttacttc tgtgatggaa
      781 atgatttttc cggaacaagg tgcataaaaa gtttcttgtt ttttcttttt gaaaatttca
      841 aacatattaa aaacctccta attatctttt cctaatattt gattgatttc ttgacttaat
      901 aagtcagctt tccctccaaa aacagcttgg atgccaccct tcacttcgaa gacagcagtt
      961 gcaccagtgt cttttattgt ttgtttttga ataatctccc cattcgtaac agcaacacgc
     1021 aaacgagttg cacaagcttc aacagactca atattttctt ttccaccaag agcttcaata
     1081 atgataagag actcttcatg taatgatgta tttttgttta ttactttttc agaatgaact
     1141 tctgattctt cttccattcc aggaatagct accttaaatt ttttgataaa gaatgtaaaa
     1201 acaaagaaat atataaatgc ccatgcaata ccaaaaggaa ttaattttaa ccaattagtt
     1261 ttgtcattcc cttgaagtac accgaataat aaaaagtcga tcaaaccacc tgaaaaggaa
     1321 ttccctacac gaatatttaa gatatcagca aaataaaaag ataatccatc taagaatgca
     1381 tgaatcacat aaagccaagg agcaacaaat aaaaacatat attcaactgg ttcagtaatt
     1441 ccagttaaaa atgatgtaag ggccccgcta aaataaagcc caccattctt tttacgattt
     1501 ttcttaggaa ttgcacgata cattgcataa catgctgctg gtaatccaaa catcattgtt
     1561 gcaaatcttc ctgcaaaaaa tctagtacca taagtaaaca gaccagtgtg gtttggatca
     1621 gctagttggg caaagaaaat attttgggct ccagcaattg ttgatccagc tacagtttcg
     1681 gtaccaccta aagatgtata ccagaacaaa gggtaaatgg tgtggtgtag tccaacagca
     1741 cctgttaaac gcaataaaaa accatataaa aaagttccta aactacccat ttgggcaatt
     1801 tgttcaccag caactactaa accattttga attggtggcc aaattaaata aaagaaagaa
     1861 ccaataatga ttgcagctaa actagaaaca atcggaataa atcttgatcc tccaaaaaaa
     1921 cctaagaatt gtggtaattc aatttttcta taacgattgt gtaaaaatgc aacagtacca
     1981 ccaacaacaa ttgatcccac tacacctgta tctagtgttg catcttttgc tgaaaaaagt
     2041 tcaagaaacc cactaatagt tgcagtatat actagataag ctactccacc agctaaacca
     2101 gctgttcctt tatctccatt tgcaagtcct acagcaatac caattgcaaa aattagtgct
     2161 aggtttgcaa aaactgcatt tccagcataa ctcataatac ttaaaattgt atgcaaccac
     2221 ggttgatcta aaaatgga
//
LOCUS       DASNNU010000179         2189 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.186, whole genome shotgun sequence.
ACCESSION   DASNNU010000179 DASNNU010000000
VERSION     DASNNU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.186"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..914)
                     /locus_tag="U8116_002743"
     CDS             complement(<1..914)
                     /locus_tag="U8116_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEN8435665.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="U8116_002744"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="U8116_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEN8435666.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="U8116_002745"
     CDS             2006..2176
                     /locus_tag="U8116_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435667.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT          743 a          384 c          419 g          643 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaatt
//
LOCUS       DASNNU010000180         2177 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.187, whole genome shotgun sequence.
ACCESSION   DASNNU010000180 DASNNU010000000
VERSION     DASNNU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.187"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            12..1082
                     /gene="xerS"
                     /locus_tag="U8116_002746"
     CDS             12..1082
                     /gene="xerS"
                     /locus_tag="U8116_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEN8435668.1"
                     /translation="MYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKNYKLFY
                     EWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNNNLGLS
                     TSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARANAIEKK
                     LFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSEVININ
                     LDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKALFLAIY
                     QGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQLGHSS
                     TNTTTLYTHIDNAATIDALNAL"
     gene            complement(1366..2031)
                     /locus_tag="U8116_002747"
     CDS             complement(1366..2031)
                     /locus_tag="U8116_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN8435669.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
BASE COUNT          770 a          369 c          327 g          711 t
ORIGIN      
        1 aaaatacaga aatatatcaa aaaatagata caatcatact tcatatgccc gattatataa
       61 aagattttgt tcaagatcgt gaggataaag atcaatcacc tcgaaccact ttagaatatc
      121 taaaaaatta taagctcttt tatgaatggc ttctgagtga gtctatcgtt cctgatactt
      181 ctatcacctc gataagacag ttaactgcct atgacctaaa cttgtacaaa tctcatttga
      241 aacgtcgcac aaaagaaaat gtaaaaaaag agacaacaaa aactaagctc aaagataata
      301 ataacttagg tctatctact tctactatca atagaaatat cacggcatta aaggttctat
      361 ttaaatatct ttcgaaaagt tccaataatc ctctaggaaa accatactta gacaataatc
      421 ctatggatca agtagcaacc atcacagata aaactacgtt agctgcacgt gccaatgcca
      481 ttgagaaaaa attatttcta gataaggata cacaaaatta tctagattat attgccaatg
      541 aatataaaaa tactttgagt aaacgagctt tgatctatta tcaacgagat gtagaaagag
      601 acctggcgat caatgcactg attttaggat ctggattgag actctccgag gttataaata
      661 ttaatttaga tgacctatct ttagataaga ataatgtggt agtaactaga aaaggaaata
      721 aaagagatgc tgtcaatata gcagcattcg caatggaata tttagctaat tatctagcta
      781 ttcggaaaga acgatataaa gtaacagaaa acgaaaaagc tttatttcta gcaatttacc
      841 aaggagaagc caagcgtata tccggtattg caatcgaacg gatggtagca aaatattcaa
      901 aaggctttag aattcaagtc agccctcaca aactgcgtca tacattagct actagactct
      961 accaacagac aaattcattg gtattaactg cccaacaatt gggacattca agtaccaata
     1021 caacaacttt atatacccat atagacaatg cggctacgat cgatgcatta aatgctttat
     1081 gagtataatg tataaatagg cggaaataag aaacaacacg ctagaaaagc ctgtattata
     1141 gtttttctag tgtgtttctt ttggtgtgat cctacttttg tttatcattt attttttatt
     1201 atcgtaaata ggtgtcggtt tgattttaga aaaattagtt tggatataat ctgtgggatc
     1261 atttgttatc tctctcaatg aatttagata caaatgacca taagttccta aagtcgtttg
     1321 tacatcagca tgttttaacc tttctttaat tcgaagtgca tctttttatt gtgatataag
     1381 caaagaagca tgactatgcc ttaatccgtg tatgcggatt atatgaacac caacgatcgt
     1441 tgcatgtctt ctcataatat gacggattgt atatttctga gtaggtgttc cattataaga
     1501 aagtacaaac gacatgcctc ctattgaaac ttgacgctcc ttccaagctt ttaaaaaggt
     1561 aattgtattg tcgtctagtt cgattgtacg aatactagaa gccgtttcgg gttctacaaa
     1621 ctcataggtt ttggctctct tataatagag tgttttattc acacgcaagt ttttattttc
     1681 aaaatcaata tctttccaag ttaatgcagt ggcttctccc aaacgcataa cggtcataaa
     1741 aagtaaccac atacaaataa aaccaaaatg ttggtacaaa tcatcaatat aaaaagttga
     1801 aataactttt gtaaattctt cttttgtcca aaaatcaact gtagttttcc ttttcttcac
     1861 atttccaata acttttgcag gatttttttc aagtagtccc aaaacaactg ctcgatctaa
     1921 tgacatctga aaaatcccat acatgtttct tacataagaa tttttatatt tgctcaataa
     1981 attattttgc cacttttgaa tgtcaattgg acgtatttct cttaactcca ttttaaagaa
     2041 ataagaaaag tttttttgat tgtagataac cgatttttat atgttctttc ttctactttc
     2101 ccttgataat aaggtaggaa taatttataa aaaaactcct caaacaacat agtactttga
     2161 gaagtttctg cttcttt
//
LOCUS       DASNNU010000181         2034 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.188, whole genome shotgun sequence.
ACCESSION   DASNNU010000181 DASNNU010000000
VERSION     DASNNU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.188"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            184..573
                     /locus_tag="U8116_002748"
     CDS             184..573
                     /locus_tag="U8116_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN8435670.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            560..>2034
                     /locus_tag="U8116_002749"
     CDS             560..>2034
                     /locus_tag="U8116_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN8435671.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPLSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT          648 a          348 c          441 g          597 t
ORIGIN      
        1 aaatatcggc tgatcagtgc gggtcggtag atgaacccaa tatcaagcca agaatatgta
       61 ctgtgatgga aggtggcgag gatgctttta caaatagagc gaaacgctat ggttttaaac
      121 aaattgtaga ataacactag agggtgagac atattattat gtccgccctt tttaggagga
      181 aaaatgaaaa aaataaaaag ctataccagt atctgggcag ttgaaaaagt catttacgca
      241 atcaatgatt ttcagttgcc ttttcctgtg acttttaacc aaatggcttg gttcgtttta
      301 tctttactgt ttgtcatcgt atttgcccat gtgcctccac tatctatgat agaggaggtc
      361 tttttaaaat acttaggcat accggtggct gtcacttggt ttatgtcgca aaagacgttt
      421 gatggcaaga aaccactggg atttttacga tcctttatca gctatcactt gcgatttaag
      481 gtgacttttg caggaaagaa agtcaaagaa cagaagaaac gatgggatga acccagtact
      541 cttgtaagga gtgtgaacta tgtatccgat taagtatatt gaaaataatc tcgtctttaa
      601 ccaagaaggc gagtgttttg cctactatga attagtgcct tacaattact cctttttgtc
      661 acccgaacag aaatttcaag tgcatgataa ctttcgccaa ctaattgcgc aaaatcgtga
      721 aggcaagatc catgcgttac aaattgccac agaaagtagt attcgcgcca cacaagaacg
      781 atcaaaaaaa gaaattaccg gtagactcaa agaagtagcg aaacaacgaa ttgatataca
      841 gacagatgca ttagtatcca tgattggcga tagtcagatt gattatcgct tttttattgg
      901 ctttaaacta attgcgacag atgaagaagt caatctaaag agtctcaaga aatctttctt
      961 ttctgggctt caagaatttg tctatggagt gaatcatcat ttgatgggcg attttgtttc
     1021 cttatccaat gaagaaattc gctgctattc taagcttgaa aaattaatgg aaagcaaatt
     1081 ggctcgaaga tttaaagtca ggcgagtcac accgagtgac ctgacgtatt tgattgaaca
     1141 tatttatggc gaaaaaggga taccgtttga agaatacgaa tttcagctac caaagaaaaa
     1201 gctaaaatca gaaacactgg tgaaacgtta tgacttatta cgtccaaatc gttgtttaat
     1261 tgaagaaaaa ccaaggtgct tacggatgga acatgagaat catgagtcgt acgtagcgta
     1321 tttaacaatt aatacaattg tgggggagat ggaatttcct tcctcagaac ttttctatta
     1381 ccagcaacaa caatttacat tcccaatcga cacaagtatg aatgtagaga ttgtgacgaa
     1441 taaaaaggct ttagctaccg ttcgtaataa gaaaaaagaa ctgaaagatt tagacaatca
     1501 tgcttaccaa tctgacaatg aaacgaattc caatgtctta gatgctttgg attccgtgga
     1561 tgaattggaa accaccttgg atcagtcaaa agaatctatg tacaaactga gttatgtggt
     1621 tcgagtgagt gccgaatcag tggatgaact aaagcgacgc tgtgatgaag tgctcgattt
     1681 ttatgatgat accaatgtaa aactagtccg gccctttggc gatatgatgg ggctacatga
     1741 agaattctta cccttaagta aacgatatat gaacgactat attcagtatg ttacgtctga
     1801 ttttttggcg gggcttggtt ttggggctac tcagatgcta ggagaattag aagggattta
     1861 ctttggctac aatgtggata caggtcgtaa tgtttacctg aaaccggcat tggcttcaca
     1921 aggagtcaag ggttcagtaa ccaatgcgct ggccgccgct ttcctgggtt ctcttggtgg
     1981 aggtaaatcc tttagtaaca atctcttagt ctactatgct gttttgttcg gtgg
//
LOCUS       DASNNU010000182         2019 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.189, whole genome shotgun sequence.
ACCESSION   DASNNU010000182 DASNNU010000000
VERSION     DASNNU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.189"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            100..570
                     /locus_tag="U8116_002750"
     CDS             100..570
                     /locus_tag="U8116_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEN8435672.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="U8116_002751"
     CDS             792..1895
                     /locus_tag="U8116_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN8435673.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DASNNU010000183         2018 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.190, whole genome shotgun sequence.
ACCESSION   DASNNU010000183 DASNNU010000000
VERSION     DASNNU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.190"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            635..967
                     /locus_tag="U8116_002752"
     CDS             635..967
                     /locus_tag="U8116_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEN8435674.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1586..1927
                     /locus_tag="U8116_002753"
     CDS             1586..1927
                     /locus_tag="U8116_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEN8435675.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          701 a          271 c          428 g          618 t
ORIGIN      
        1 caaatataga aaagggaacg tatgtttgca tactcctttt ttttgagtta taataatacg
       61 cgaagagaga taatttcatt atttaaacaa gctaaaaatc ttgatattac ttcttttcag
      121 gcaatacgaa tattattcgc ttacattttt tgtataaatt taattgtctg ctatgcttgt
      181 gatgaattga ttttcgtttt gcacttttgg atgataaatt tggacaattg aaaatacaaa
      241 aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt cgtcagcgtt catacacggc
      301 attaagattt ggcccttaat gcgttaacct ttcagtgtta cggtattacc cgcaatggat
      361 ttagtataac agatattcaa aagtgatacc actttttttg gctgtgttaa ggttaaaaat
      421 acgtgatatt acgatgaatc tatgcctaag cataatgtta taaaaatgtc cgtgtggctc
      481 tttgccgtat gacgaacgct gaaattagag ggaacgcatg tttggatgcg ttccctcttt
      541 tttgtttgtc atgaacgaaa atcaattctc tatttttgaa aaataacaag aaaaacttgt
      601 taaaaatatt taaagaaatt gggtggttta ttttatgtca tatacaggaa ttttgagtct
      661 caaagatatt tgtcactacg gtaaacggtg taccgctaca gagaaaatca cgaagaaact
      721 atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa tatataatcc agaaagacaa
      781 agtctcagag gaaatgattt attacattgg gaaacaaaag caaataattt taaaagatcc
      841 tatcccctta aaagagctat atccaacaat caaacatgtt tatgaccaaa acggtgtact
      901 gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg ggcgtttgat
      961 cggctgaatt gatcattctc ataacttgta ttttatcgtt tttcgcttgc ttgaaaatat
     1021 tgtcgagact tctaaaggaa cttagagtca ctagaaaaat aaggagtaga cttgatctat
     1081 caacaattcc cttcggggag tgacggggta ggttttaacg attggtaacg taaaaaattg
     1141 gtcaatcgag taaatgcgct tgtttggata gtggtgaaat gaatttgtgt aaataataag
     1201 cgcagagtaa acaagaggga atttagaagt tagacaagtt agaatagcgt ctagattaat
     1261 catgagcagc tactaaggga tctagacttt gacgcaaact catggaataa ggagtaggtc
     1321 ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt gctgcagtac atgtagatac
     1381 ttgcgaggac gttgattatt ggatcaatcg agcatttacc gctgtctact agggtttctc
     1441 gtttcggacg ccataaacga gtcgaaattt tttatgtatt tttaagtaaa cgaaaaacga
     1501 taaaatatga acaagtcctg aataaagtga aaaaattaat ataaagcata gagaaaatga
     1561 aatttacgta atggaggaat gacaaatgaa aaattatgat ccgaatatac gattgggcac
     1621 acatactatt aaggtttctt tccaacgttg ggattacaaa ggatttgtga cgtttagaag
     1681 gggcggaaat tgtaaaggat tagatgtttt agcattagac gaggatgatt tatacgatca
     1741 aacattgact gataatccaa ttggattcgg cttgctacct gaagatgatg aaggtaatga
     1801 atggtttaaa atgacgctta tgaatgataa tggagacgaa ttatctgttg aagatacttg
     1861 gtcatatttg agtgattata ttgtcagctt tgaaatcatt gaatttgtag cggataaaga
     1921 agaataaaaa ctggaggaat aaattttaca gataaaggct tagaaggagt cacgacgatt
     1981 aataaaaagg tagtattacc ttaggcattg agtaagga
//
LOCUS       DASNNU010000184         2003 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.191, whole genome shotgun sequence.
ACCESSION   DASNNU010000184 DASNNU010000000
VERSION     DASNNU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.191"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..378
                     /locus_tag="U8116_002754"
     CDS             <1..378
                     /locus_tag="U8116_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN8435676.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="U8116_002755"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="U8116_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN8435677.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="U8116_002756"
     CDS             1859..>2003
                     /locus_tag="U8116_002756"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435678.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DASNNU010000185         1939 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.192, whole genome shotgun sequence.
ACCESSION   DASNNU010000185 DASNNU010000000
VERSION     DASNNU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.192"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            316..933
                     /locus_tag="U8116_002757"
     CDS             316..933
                     /locus_tag="U8116_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN8435679.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            974..1273
                     /locus_tag="U8116_002758"
     CDS             974..1273
                     /locus_tag="U8116_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEN8435680.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1307..1474
                     /locus_tag="U8116_002759"
     CDS             1307..1474
                     /locus_tag="U8116_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN8435681.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1519..>1939)
                     /locus_tag="U8116_002760"
     CDS             complement(1519..>1939)
                     /locus_tag="U8116_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN8435682.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          651 a          309 c          379 g          600 t
ORIGIN      
        1 aaaaattgta aaagttaaag aagtcaaatt ctgatgtgaa aaataaaata taccatggaa
       61 gacataaaag aaaaactgga taactagttt atttaatggc agaccgttaa ggcctgccat
      121 ttttatgtat tttagcggaa taaatataat catgcgggag tgtaaactta ctgtttcaaa
      181 aagtatactt tttgacatgc cttgattttc tttgtttatg tatggcataa taaccacata
      241 atacaatttg aaacgtttct gatttaatct gattgttttg aaacaatttt agtgaaaaaa
      301 gggtagataa aaacgatgag aaaatttttt tatatccggg tcagtagtta tgatcaatcg
      361 atcgaacgac aactgatttc cgcgaaggaa ttaggaatcc ctgaagaata tatatttgta
      421 gaaaaagcca gcggcaaaga ttttaaacgc cctgaatatc aattaatgaa acggatgttt
      481 cgaaaaggag atttactcta tctacaatca ttagatagac taggcagaaa taaacaaatg
      541 attttagaag aatggcgaga attaactaag gaaaagcaga ttgatatttc tgtgttagac
      601 atgcctctgt tagatactgc tagttacaag caactagatg ggttagagac gcttatctct
      661 gacttaattc tgcaattact cagctatatg gccgaagacg agcggaaacg aattaatagc
      721 cggcaaagag aaggcgtaga agctgcgaaa caaaagggtg taaaattcgg cagaaagaaa
      781 attgctttac ctcctgaatt tcctgcgatc tattctgact ggaaagcagg aaagataact
      841 gcagtctatg ctatggatca attgggcatg aaacgaaata ccttttatcg acgggtgaaa
      901 gaatatgaac ataccttgca ccacttttct tgatagatat aacagctatt agacgggaag
      961 ggagagtttg aaaatggact taacgaatca aagggtcgtt tttactggaa cgctgcagca
     1021 aatgacacga acccaagcca tcggtttggt tcactcttta aacggccatt gtcaatcaag
     1081 tgttacttca aaaacaaatt tcctggtttg tggtcagtat tctaagtcgt tgtttgatga
     1141 ttctctctat acaaaaaagg aacaaaccgc gcgggacttg attgcgttag gtcatgagat
     1201 taccatttta tcagaagtcg aattttatgc gttaattagt caacagttga aagaatggca
     1261 acggtattta taatcgttag taaaatttac aaggaggaaa atttttatga agcgaaaatc
     1321 ttatgatcgt tggggcattg aaataactag ttttaatcga aatattgctt ttcacgtgga
     1381 gtgtgactgt ggggaattag caaaaagtgt ttatcaaaaa aattactttt actgtcctaa
     1441 atgccatcgc cgatacgggg tacatggaac atagaaacga aatagtgcgg tagaaaagcg
     1501 aatgaatgta gtatatttct acaaatcatt cgctttcttt attttataaa tagttggtcg
     1561 agagatcccg ttttcttttg caatctctgc aactggaatg ccttgcttta acatgtccac
     1621 cacggtttta aaaactaagc gtttttgcgg atctgttgag ttaatagaat actcgattgg
     1681 tcgtccctta tatcggccgg ctttttgagc caataatact ccttgccgct gacgttcacg
     1741 aattttctca cgctcgtttt gtgcaatata acttagcagt gataaaaaca tatcgaacat
     1801 agcggtatcc aggagttcat ttccagtatt aaaatctaaa aactgtgcat ctaatatttt
     1861 taacgctact tttttctgtt tcataaaagc aactgtattt tttatatctt cataatctct
     1921 tcctaaacga tctagtgat
//
LOCUS       DASNNU010000186         1854 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.193, whole genome shotgun sequence.
ACCESSION   DASNNU010000186 DASNNU010000000
VERSION     DASNNU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.193"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1155)
                     /locus_tag="U8116_002761"
     CDS             complement(<1..1155)
                     /locus_tag="U8116_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN8435683.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
     gene            complement(1158..>1854)
                     /locus_tag="U8116_002762"
     CDS             complement(1158..>1854)
                     /locus_tag="U8116_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN8435684.1"
                     /translation="KLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERL
                     KIQYGTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGD
                     LRIVKEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNE
                     VNAPYRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFD
                     FDSKIWVITQEEK"
BASE COUNT          625 a          335 c          277 g          617 t
ORIGIN      
        1 tctagtattt tttttatttg tcgcaaaaaa tgttacaaat aggatcacaa taaatattcc
       61 tgcgaataca aaaaatggag cagtcaaatt tgtacttctt gttagaaatt gtgctaattg
      121 catagcaata ggagctataa aattaccaat attacagcca ataaaaatca atgaagtagc
      181 aaaattaatt gtattctttg tagtgacttt atctaagtta ctaaagataa atgggaagca
      241 ccatgatcct gttatacttg ataaaaataa acccgttagc aggaacgtca tattaccgtt
      301 tgacacagca attaacaaat ttgaaattat aaagaatata attcccaagt atagggttcc
      361 ttttcctatc cacttattta ttggtccaaa cataaatcct gctagtattc ctaagatagg
      421 cattagagct aaatagttac tagcattaaa gtttacccct ttaatttcag ttgcaattga
      481 tgcaaatcgt acagaaatag caatactatt cattacgagt aagattgcaa atagaactag
      541 ggcaaaaacc attggattca tcttttcttt tacatgattt tcaggtatgt cactattttc
      601 atctctaatt tcaggaactt ttagcgcgaa taagagagct acaggaaacg caattgcata
      661 gactaaaaac gaagccgtcc aattaatgtt cactaaaact ccagcaagaa aaataaggat
      721 tgtctgacct aaagcttcca ttgaatttct aatacctaat agcgttgctc tagtattgcc
      781 agagtataaa gagctgatat atgtcacagc caatgaatta tatactccta aaccggcacc
      841 taatattact cgactaataa gaacggtttg gaaggtagct gtaaatactg gaataattcc
      901 tcctatacca gctaataata aacctaaaat tattgtcttt ttcattccta ttcttttggc
      961 aaagtaacta gataataaga taaacaaaat aacagttata cttggtgcag ttcctaaaag
     1021 ttcactttga ctttgtgata tccccaaaga ctgtttcatt tgtggaataa caccattaat
     1081 tgcttgagag cttgttaaca ttagtgaaat acttaagatt gctccttttt gaagtaagct
     1141 tttttggtta ttcatctcta cttttcctcc tgtgttataa cccaaatctt ggaatcaaaa
     1201 tcaaatgttt ctgggcgtcg attctgatca tttatttgtc cactagatgc atctgaacag
     1261 actagtcctg ctctcatcaa ttccattcca gatcgcaaac tattttgtcc tacattataa
     1321 agtccgtcat tatctaaccc ttttaatttc agtcggcggt aaggagcatt tacctcattt
     1381 aatgttttat aatgagcaac aatagccgtt ttcttatcat gactaacaac catccacgcg
     1441 gtttgattat catcatcgaa tggtgataat agtctataaa aagtaccatt atgaattatg
     1501 gttctatatt ttttataaaa ggcaatttgt tcttttacaa tcctcaaatc gccttcactt
     1561 aattcattta agtcaagctc atatccaaat gcaccaaaaa aagctacatt tcctctagtt
     1621 tttataggtg ttatacggtt tgtttgatga tttggaacaa ttgatacatg agcaccaata
     1681 gacgaaagag gatacagcat actagttcca tattgaattt ttaacctttc aattgcatct
     1741 gaatcatcac tagcccaagc ttgaggtgca tagtgaagca ttccaggatc aaagcgccca
     1801 ccacctgatg aacaagactc aaatagtatg ttaggatatt ttgttataag tttt
//
LOCUS       DASNNU010000187         1698 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.194, whole genome shotgun sequence.
ACCESSION   DASNNU010000187 DASNNU010000000
VERSION     DASNNU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.194"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(27..1508)
                     /locus_tag="U8116_002763"
     CDS             complement(27..1508)
                     /locus_tag="U8116_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8435685.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          524 a          231 c          293 g          650 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      721 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      781 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      841 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      901 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      961 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
     1021 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
     1081 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
     1141 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
     1201 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
     1261 ttagaaaaaa cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta
     1321 tgattagaat aaataaattc atcaatcaat aaaaattgct tgagtgatac atttattttt
     1381 cggatcagtt ctttgattgt ccgttttgga atatgtaagt cattggataa ttcttgtaaa
     1441 gaacaattat ttttttgaat gaccatttgt agaacaagta actctttttt tcttgcttca
     1501 attagcataa atacctcctt tttattctgt ttttgtatat agttagacaa aaactgtgag
     1561 tggaaagtca tttcagtctg tgttattctc acagagtgtc gccttctttt ttatataacg
     1621 ttatttaatt tttttgttac cttgtttatt ctattgttta tttcctaggg tattttgtca
     1681 agatattctt gtttttaa
//
LOCUS       DASNNU010000188         1685 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.195, whole genome shotgun sequence.
ACCESSION   DASNNU010000188 DASNNU010000000
VERSION     DASNNU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.195"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            437..1567
                     /locus_tag="U8116_002764"
     CDS             437..1567
                     /locus_tag="U8116_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN8435686.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          486 a          272 c          350 g          577 t
ORIGIN      
        1 aacttatttg ttattttttt ctaaaaaata ttaaagattg ttaaatttca ctatttcatt
       61 tcaaattcca ttgcctaatc attaggaaaa gtctatcatg aactcgtaaa tagaatatag
      121 taataacctt gttaggtgag gctcctatac aaacataggc tgttgcccag aaacgtcgaa
      181 agtcgccaat gggtaaaaca gaaactgttg gattaaggct ttttccaata gctaagaatg
      241 ttttctttac gttgtatagt gctaaaactc ggacgatgag atgatgctag gtcagaattc
      301 ttgttcgaaa aggtttctga ccttttcttt atatttttgt gactgactta tttgatgaga
      361 caaactcgct cctcacctga caagtggatt gctgtgtgag gaggagatgc ggtgaaaaaa
      421 gatcgattgg tggattttgg gtccttattt gactttgtcc atgatcggtc ttttagaagt
      481 atacagtgcc agttcttatc gtttattgca agccgatgaa aatacaaaaa gtctattatt
      541 gcgtcaacta attttcatat ttttgagttg gagcgtgatc ttcttagctc gttcagtcaa
      601 actacactat ttacttcacc ctaaaatagc aggatacggt ttagccttat cgattttctt
      661 tttagtatta gtaagaatag ggatattcgg tgtcactgtc aacggcgcac aacgttggat
      721 ctctctgttt ggcattcaat tccagccttc tgaactggca aatctttttt tgatttttta
      781 tttaagctgg ttttttcgtg acggaaatag tagcccaaaa gatctaaaaa aaccattcct
      841 gattacagta ggtataactt ttctgatttt atttcagcca aagattgctg gagcattgat
      901 gatcctttcg attgcgtggg tcatattttg ggcagcggcg gttccattta aaaaagggat
      961 ctatctaatc gttacttttt ctgcattgct gattggagca gcaggcgggg tattatattt
     1021 aggaaataaa ggttggcttc cacagatgtt taatcatgct tatgaaagga tcgcgacttt
     1081 aagagattct tttatcgata gtcatggtgc gggctatcaa atgacccatt cgttttatgc
     1141 actatataat ggtggcatat ttgggcgggg attaggaaat agtatcacaa aaaaagggta
     1201 tcttccagag acagaaacag attttatttt ttcgattatt acagaagagc ttgggctaat
     1261 cggtgcattg tgtgtgcttt tcttattgtt ctcattatgc atgagaatct tttgtttaag
     1321 cagtcgctgt aaaaatcagc aagctggatt gtttctttta ggattcggaa ctttgctatt
     1381 cgttcagacg ataatgaacg tgggaagtat cgcggggctg atgccaatga ctggtgtgcc
     1441 attgccattt gttagctacg gaggaacaag ttatctgatc ctttctttag gaatcggaat
     1501 cacgttgaat atttcttcaa agatacaggc cgaagagttg gcgctctatc gacctgaaaa
     1561 acaataaaga gaaactgaga aactctcagt ttgaattatc ataaagtaca atcttttatt
     1621 tttcaagcga gaggttcttt tagaaagagg aattcgcaat gaaagtatct caaaaaatca
     1681 tatta
//
LOCUS       DASNNU010000189         1637 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.196, whole genome shotgun sequence.
ACCESSION   DASNNU010000189 DASNNU010000000
VERSION     DASNNU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.196"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(183..1502)
                     /locus_tag="U8116_002765"
     CDS             complement(183..1502)
                     /locus_tag="U8116_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HEN8435687.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          526 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaatt
//
LOCUS       DASNNU010000190         1636 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.197, whole genome shotgun sequence.
ACCESSION   DASNNU010000190 DASNNU010000000
VERSION     DASNNU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.197"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(73..450)
                     /locus_tag="U8116_002766"
     CDS             complement(73..450)
                     /locus_tag="U8116_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435688.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(750..1631)
                     /gene="galU"
                     /locus_tag="U8116_002767"
     CDS             complement(750..1631)
                     /gene="galU"
                     /locus_tag="U8116_002767"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN8435689.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          451 a          367 c          242 g          576 t
ORIGIN      
        1 ccaattaaat aaatctctca tctcaatagt ttatccatta ttttatcttt ttttgcgtat
       61 acctactaaa tattaccgtc ttcttctcgt tcttctactt tgttcgagag aatctgtact
      121 agaaaaagta gttgtatgtc taccttttct gcctatccga gactttcttt tcatatatgg
      181 gatataaatg attagataca gtacagcaaa aaataaagca aatacgcaag cttcccaact
      241 acctaacgcc aattcaacca tcaaggtagc aaccccaacg gatgctgcat accagataaa
      301 aatcagaaac gtttcttttt ttctgaccat gcgaaacatg ataaatagac taataagcaa
      361 tagtggcaac acaaatatcc aattgcttcc ttccatcagc cacttgagta tatcaagcag
      421 cgactgtgca acagcgacta attttcccat agtgaacaca cacctttttt tataattaaa
      481 aaattcgcct ttttattcta ctatgaatct tctggcttgg ctttcatttc tcttatgtaa
      541 tcattttctt ttatacaact taaaaaaaaa ttaaaccatt agcataatat tttctgaatc
      601 tattcacgaa agtctttttc tatctttcgt tttccacgac tcaactgatc tagagtcaaa
      661 aaactaaaac gacagttgcg caaatgacgc acaagctgcc gctttaataa caaatagcct
      721 ttcattctca gcaggccact tatttccagt taactttctg ttcttagctt tttactcaac
      781 tggataatat atgcacttaa tgaagtccca atttccgaat gttgaagtcc atattcaata
      841 tttgtcttca tatatcctaa cttatcgcct acgtcgtatc tttttccttt aaactctctc
      901 gcatagaccg cctctgtctg gttaagagtc tgaagtgcat ccgttagctg tatctcgttt
      961 cctgctcctg gttcttgagt ttctaatatc tcaaatattt taggagtcag tagataacgt
     1021 ccgataatgg ctaaattact tggtgctttt cccatttcag gtttttctac aaaccctttt
     1081 actctgcaac gacccttttc gctatctgtt ttaggatcaa taattccgta tttagatgtt
     1141 tcattttctg gtacacgcat gacggcaaca gcggaagctt gtgtttcttc ataatccatc
     1201 atcaattgtt gagtcaatgg gatatcatct ttcatcaaat catcccccag catgacgaca
     1261 aacggttcat ttcctataaa tgcttttgct tgaagcacgg catcccccag tcctcttgga
     1321 tgagattgac ggatgaagtg aagattgact tgagtcgttt cttcaactaa agcgagtaat
     1381 tctgtttttt ccttttcccg caaattgttt tccaattcga tatttgcatc aaagtgatct
     1441 tcaatgggtc ttttttcttt tcccgttacg atcaagatgt cttcgattcc cgaatccaat
     1501 gcttcttcca caataaactg gatcgttggt ttatccacga ttggaagcat ttcttttgcc
     1561 atggcttttg ttgccggcaa aaaacgtgtt cctagcccag cggcaggaat gactgctttt
     1621 ttgaccttca cttgct
//
LOCUS       DASNNU010000191         1623 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.198, whole genome shotgun sequence.
ACCESSION   DASNNU010000191 DASNNU010000000
VERSION     DASNNU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.198"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..784
                     /locus_tag="U8116_002768"
     CDS             <1..784
                     /locus_tag="U8116_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HEN8435690.1"
                     /translation="IKKYRDELFDWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEK
                     SRRQTSSQEIDQLIHEMKTILKKADPYSDMMKLNELIQEFIEQYNQLLELESQEILNK
                     LTEEKQATFKLLEGKDFKDQFVPIVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLI
                     FNQRFDNEENRRRQEEQARKQIEAANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVP
                     MRTLNVHVKTIKSAADIDELTAEINQKLKEQLQENTEITIQF"
     gene            848..>1623
                     /locus_tag="U8116_002769"
     CDS             848..>1623
                     /locus_tag="U8116_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEN8435691.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPA"
BASE COUNT          670 a          213 c          303 g          437 t
ORIGIN      
        1 aatcaaaaaa tatcgcgatg aattattcga ctgggctgaa acatatgata aaattaaaaa
       61 tttccacaag caaaatagta ctcagaaaaa aatctgggag aatgcacaag ataaaattag
      121 attaatcgaa aaaagtcggc gtcaaacttc tagccaagaa attgatcaat tgattcatga
      181 gatgaaaacc attttgaaaa aggctgatcc ttattcagat atgatgaaac taaatgagtt
      241 gattcaagaa tttattgaac aatataatca actgttagag ttggaaagtc aggaaatatt
      301 aaataaatta acggaagaaa aacaagcaac atttaaatta ttagaaggaa aagattttaa
      361 ggatcaattt gtaccaatag ttgaccaaga atttcaagaa ctgaaagcaa aaattgaaca
      421 agcgaatact atagatgatc ttgttttctt atcattaaac gtagcagaac agtatttgat
      481 ctttaatcaa cgtttcgata atgaagaaaa ccgcagacgt caagaagaac aagcacgtaa
      541 acaaattgaa gcagcaaact tgattcgtga aactgaaaaa gatgaaatag actctgcagc
      601 aaaagctgaa gaaatagcga tagtaaaacc tactattcaa cagaaaacag taccaatgag
      661 aacacttaac gttcatgtta aaactattaa gtcagcagca gatattgatg agttaacagc
      721 tgaaattaat caaaaactga aggaacaact tcaagaaaat acagaaatca cgattcaatt
      781 ctagtgaaaa gacaggcata aaaaggtgcc tgtcttttca aaggttacat atagaaggga
      841 aagaaaaatg gataagaaag cgataaagaa tttcgcggtc aatgcccgta gaaaattaat
      901 tgatgaagta atgactaaag caaaacgttt aggaattgat gaatcaaaaa ttgaagatgt
      961 gaagaaaatt gatagcaatt tacaagaaat cgaaaaatca ggaataagaa ttcaaggaaa
     1021 agaaatagcg caaagaaaca aattgattgc agaactaaaa caacgtgctt ctgtcacttc
     1081 ttattcagaa gcttttcaag agttaatcga agaggttgct tatacgtggt ttaaccgatt
     1141 gattgccatt cggtttatgg aggtaaatga ttatttacct gacacgtatc gtgtattatc
     1201 tagtgaaata agtgggaaaa ttgagccaga tattattaca gatatttatg atgccgatat
     1261 ctttgaagaa ttatctagta atgaacagca acaagtcaga acttggaaaa cagataattc
     1321 agcagaagca atggacaagc tgtatcaatt gatttttata aaaatctgta atcaattgaa
     1381 tgattactta cctgagctat ttgaagaagt agaagattat acagaacttt tgtttacagc
     1441 ttcttatatt aaaaaagatg gtgtaatcgc tgatctttta agtattccag aggatgattt
     1501 ctatgttcaa gctggtggac aggtagagat tattgggtgg ttgtatcagt actataatag
     1561 tgagccacat aatcaagtgg tgaatattta taaagggatt gtcaaaaaac aagatattcc
     1621 ggc
//
LOCUS       DASNNU010000192         1614 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.199, whole genome shotgun sequence.
ACCESSION   DASNNU010000192 DASNNU010000000
VERSION     DASNNU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.199"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            187..450
                     /locus_tag="U8116_002770"
     CDS             187..450
                     /locus_tag="U8116_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN8435692.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            450..722
                     /locus_tag="U8116_002771"
     CDS             450..722
                     /locus_tag="U8116_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN8435693.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            799..1128
                     /locus_tag="U8116_002772"
     CDS             799..1128
                     /locus_tag="U8116_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN8435694.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1118..1404
                     /locus_tag="U8116_002773"
                     /pseudo
     CDS             1118..1404
                     /locus_tag="U8116_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          593 a          205 c          306 g          510 t
ORIGIN      
        1 tttgttgatt taccaacatg tttagacatt ttcctctagg aaaaaattaa ctagcaccac
       61 gggtataaag gaaacggata acactttaca atgctgtttt ggtactccaa tttaaacaaa
      121 acaatcgaat ttacgtactt aattaagtac gttagaatgg agatataaat tagaggggga
      181 gtagatatgg caaaaacagt cttaaatcct agctcagcac ggaaaaattt ttataagttg
      241 ttgaaagatg taaatgagaa tcatacggaa attgaaatca ttagtgagcg cagtgaaaat
      301 aatgcggtgc ttattggttt ggatgattgg aaagcaatca aagaaacgct attacttgag
      361 caaataggca ctttgggtgt tgttcgtaag cgagaaaaag atgatagtgg atttactaat
      421 gttgatgaaa ttgattggga taacttataa tgaataacta ttctgtgatg attaaaaact
      481 cggctaaagc tgatttaaag aaaatcaaac agtctaattt gaaacctcaa tttgagaagg
      541 ttattcagac tttaaaggaa gatccctatc tccctaccca atcatttgag aagttaaaac
      601 ctacacatga aggtaggtat tctagaagat tgaatagaca acaccgggtt gtttataagg
      661 tagatgaaga tgaacacgtg gttgaaattt attcagcaag gacgcattac gagtcacagt
      721 aattacttgt aaatacatta tctcgtggta taatgtattt actaagtaat tacatactgt
      781 tagaaatgag ggatttaaat gattacaaaa acaagaaaac aaggcaattc aatcatgttg
      841 acagtaccta aagattttaa tgttcctaat ggagtcgaag ttgaagcaaa gttggtagag
      901 aatggtattc tttatgaatt tgttgaacca caaaaagaat tttatgattt tagcgaagat
      961 attttgtctg atataattgc agaaggctac gataaagatg agattcttgt agaatttaag
     1021 aatagaaaaa acaaaatgca ttcttccttt agagatattg ctgaagatac acttactaat
     1081 tctaaagtaa tgactaaaga ggagttggca acagaaattg gcttatgaaa tcgttccttc
     1141 aaaacatgtc attaagtatt taaaaagcta aaagagaagc cattgaaaga aaaatttttg
     1201 aatttaattt atgatgaaat tgcgattgat ccatataagg gtgaacaaaa aactggggat
     1261 ttatcaggta tttggtctag tgggttcaaa tatgcgggca caacatatag agtagcttac
     1321 gagattatag aaaacaaggt tattccagta ttgttatgtg gaacgcatga aaacttttat
     1381 gaacagttga aaaaattaat ctagtgattt gttgtcgttc ttaagaagga cggcttcttt
     1441 tttatcgaaa atctgattcg tctacatgac taaagtcgcg agtattctct gcttctcgat
     1501 aaattaaata acgtcattac tttgaggaaa gctataaatc aatatatttc agcttttcct
     1561 tatttatgta ctacctttta cttattaatt ataaggtagt acatatatat cttt
//
LOCUS       DASNNU010000193         1612 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.200, whole genome shotgun sequence.
ACCESSION   DASNNU010000193 DASNNU010000000
VERSION     DASNNU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.200"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..850)
                     /locus_tag="U8116_002774"
     CDS             complement(<1..850)
                     /locus_tag="U8116_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEN8435695.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYP"
     gene            complement(1009..1275)
                     /locus_tag="U8116_002775"
     CDS             complement(1009..1275)
                     /locus_tag="U8116_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8435696.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1265..1612)
                     /locus_tag="U8116_002776"
     CDS             complement(1265..1612)
                     /locus_tag="U8116_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN8435697.1"
                     /translation="MSFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNGMLV
                     AQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISELKH
                     AKELREEWFTNDD"
BASE COUNT          456 a          314 c          199 g          643 t
ORIGIN      
        1 atggatactc atcaaaagaa gaatcgtatt cttcctttaa aattcccgat ttacttaatt
       61 tttctagtac atcaaatact ctatcactca agtaactcct taaattgtta tcactttccc
      121 attgaactac ttttctataa gttgaattat cagttgaaaa gatagaattt tcatccataa
      181 acgcatatat tagcagcaat ctttcatcat tgtcaaattc tattggatca aaaatatcta
      241 agttgtcatc aaaatcttta tcagttccgg taactttttt gttaattact gaaggtgtct
      301 tgtaagaaat agaatctaaa tcaaagcgtt ttgattctaa attattttgt gctttctcaa
      361 ttaaataatt cacttttttt gtgagaaacc ttctgatatt gtctttgcta tatgttccat
      421 aaaaaccctt atcttctaaa gagtccctgt acttcttaac ttctatatac tcttcagcca
      481 aaacttgtgg aactggttca tctaagaaaa aaacccctac ctcgcctttg ctatttttat
      541 aaaagtaatc aatttctgct atagttcccg atctataatt atcaactgga gaacccaact
      601 tatggccaaa aatcgctaat aatatatcac tattttctac aatttgttta ttaataacac
      661 tttgcccaga gctattattt atataagtag gaatcgcatt cgtttcccat cgaattggca
      721 tcaaaattac ttttgtatta tctgtatttt ctatattcca tctatatatg gtttcttcta
      781 cttcattcct ctttgctgct atatcacctg gtgatgctat aaatactttt attagattta
      841 tggttttcat ttattgtacc tttctcttta tgtctcttta aagtaatttt tgtataaaat
      901 ttattgactt ttacactata ggacaacttt tatctattta gtacaaaaag gcccactaaa
      961 ctaattgatt agttaggagc ctattacagg tatattttca actcgctttt aagcaaattc
     1021 tactaccagc tttttgccta atgccctagc gatatcatca agtttatcga aagatacgtt
     1081 atcccctctc tcaattcgag caatagtaga ttgagttgta tgtgcttttt gtgcaagctc
     1141 cgcttgagtt aggcctgctt cctcccgtgc atgataaaga gcaaccgcag tttcgagttt
     1201 tcttccttca cggtcatatg cttctgcaaa atctttgtct ttttgctttt cttcaatcaa
     1261 ttttttaatc atcatttgtg aaccactcct ctctaagttc ttttgcatgt ttaagttcac
     1321 ttattggtgt tttctccgtt tttttagtaa atccatgggt aatgacatac cttccattga
     1381 ccacatggaa atagatagct ctttggatgt ttgatcctac cttacttctt aattcgtaca
     1441 aattagtgtc taattttttc acccatttca accgttgagc tactagcatc ccattctttt
     1501 ctgtttcttc tattgtacgt aaaagcttcg cactatcttt ttttggtaat gtttccaacc
     1561 attctaaaaa ttcatcatga ccattaggtc ttttaaatgt ttcaaaagat at
//
LOCUS       DASNNU010000194         1607 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.201, whole genome shotgun sequence.
ACCESSION   DASNNU010000194 DASNNU010000000
VERSION     DASNNU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.201"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            19..876
                     /locus_tag="U8116_002777"
     CDS             19..876
                     /locus_tag="U8116_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN8435698.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            877..1425
                     /locus_tag="U8116_002778"
     CDS             877..1425
                     /locus_tag="U8116_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435699.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
BASE COUNT          606 a          224 c          344 g          433 t
ORIGIN      
        1 aagaaaagag gtaggaacgt ggtagcgaag aaatatgtaa tcggtaattt taaaggtggc
       61 gtaggtaaaa gtacgtgcgc gcaaatgtta gggtttgaat cagcagttaa tgaaggaaaa
      121 agaaccttga taattgactt ggatatgcaa ggaaatactt ctgatgtgat gaacttaacg
      181 catatgaact tcactaacga agaaggaggc ggagaaggtg aaccgattgt ttacgaaaat
      241 actgtgactg acgtgttgat aaacaatctt ccacctaaag aggctgtcta taaagtaatc
      301 aataacctct atatacttcc agcagacatg tcctttgaaa tgtatgatga ttggatcaaa
      361 gaccaatata atgattcatt atcccaattc aaatatatga aagaaaagct agaaccatta
      421 tttgatgagt ttgacgtcat ctatttagat gtcccacctt ctatttctgt atatagtaaa
      481 tccgcgatgt acattgcgga ctgggcaatc gtcgtcttac agacccaagt aaaatcgatg
      541 agaaatgcga tgcaatatct tgagtatatg gacttttttg tgaaagaatt tgatacaaat
      601 ttatatgttg ctggtgttat tcctttcatg ttggaaagcg gtgacgcagt agatcaggaa
      661 atgtataaac aagcccaaga gatttatggg gaacatttga ttaaaaatgt tgtactcaaa
      721 aatgcgcgct taaaacgata cgacggatca ggtattacga tggagaagac aaagaaaggt
      781 ctcttaaaac aatgggatcg caaaagccac gaattattta tcagtatttt gaaagaattg
      841 gaagaacacg agaattggta tgcggaggtg gagtaattgg ctgacatcaa aggacttgat
      901 atttttggga acaacaaagg actcgccaaa ataaaaaaag aagcagaagc aaagaaaaag
      961 cagcaagcaa ttacaccatc tgaaaataag caattagaag tgcaccaagt tcaagaaata
     1021 aaaacttcct ccaatgttga aagacagagt agaggaagaa caaaaaaagt tggcgcacca
     1081 atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc gactttgtta
     1141 aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag agatgaattg
     1201 cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga taagcaagat
     1261 ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc aattgttcca
     1321 gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca agataccgaa
     1381 gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc aaagaagaac
     1441 taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat gaaatagagg
     1501 ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag gaacggttat
     1561 gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagt
//
LOCUS       DASNNU010000195         1594 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.202, whole genome shotgun sequence.
ACCESSION   DASNNU010000195 DASNNU010000000
VERSION     DASNNU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.202"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            288..695
                     /locus_tag="U8116_002779"
     CDS             288..695
                     /locus_tag="U8116_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435700.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="U8116_002780"
     CDS             709..1110
                     /locus_tag="U8116_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435701.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="U8116_002781"
     CDS             1112..1483
                     /locus_tag="U8116_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435702.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          595 a          201 c          277 g          521 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttactaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtcc
//
LOCUS       DASNNU010000196         1556 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.203, whole genome shotgun sequence.
ACCESSION   DASNNU010000196 DASNNU010000000
VERSION     DASNNU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.203"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            58..1524
                     /locus_tag="U8116_002782"
     CDS             58..1524
                     /locus_tag="U8116_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN8435703.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          475 a          268 c          317 g          496 t
ORIGIN      
        1 aaaaattgaa tatgattaca tctggataac aaatttttat cataaaggag agaacgaatg
       61 aacaacaaac aattacgctg gtttactgtt gcgttaattg catttaacat ggtttggggc
      121 atggggaatg tagttaataa ctatgctcag caaggagtat cagttgtcac ctcttggcta
      181 ttgattttgg ccatttattt tattccatac gcattgatcg ttggacaatt aggatcagcc
      241 ttcaaggata gtaaaggagg cgtcagttca tgggtcgaaa atacgacatc taataaacga
      301 ctggcatact atgccgcttg gacttactgg gtcgtccata ttccttattt ggcacaaaag
      361 cctcaagcaa ttctgattgc tggtggttgg gcagtccaag gaaatggaaa tatcgtaaat
      421 acgatgtcag tacaaatggt agcagttatt tcacttatta tatttcttgc ctttttatat
      481 ttatcaacaa aaggactttc tactttgaaa gtgattggcg gactggccgg aacagcgatg
      541 ttcgtcatgt cgatcttatt tattctttta gctgtaacgg caccgttcat caatccaacg
      601 atggaatttg ccacaccaca tatgaatcaa gtgaaaacat atattccaac atttgatttc
      661 agttatttca caaccgtttc gatgctagta tttgcagttg gaggggcgga aaaaatatcc
      721 ccttatgtca actctacaag aaatcctgca aaggaatttc ctaaagggat gatcttctta
      781 gcagcaatgg tagggatttg tgctgtatta ggctcagtcg caatgggtat gctgtttgct
      841 agcgataata tccctaaaga cttgatggca aatggggcat acagtgcttt tcaaatttta
      901 ggtaattact atggtatcgg taactcattg atgatcattt acgcattgac aaacatgatt
      961 ggacaaatct ccgcattagc cttttcaatt gatgcaccac taaaaatctt gttagcagat
     1021 gcggatccag aatttgtacc aaactggcta cgtaaacgta atcgtaaagg tacattggtg
     1081 aatggttatc ttctgacagg tatactagta ggaatcatta ttttgttgcc gatctttggt
     1141 atcaaagata tgaatgaatt agtaaaatgg ttgacgaact tgaactcagt tgtcatgcca
     1201 atgcgttacc tatgggtgtt cttggcatat atgatgttga ataaagctta taagaaattc
     1261 aattctgaat ataaatttgt taaaaatcca aaaatcggtt ttatctttgg tgcttggtgc
     1321 tttgccttca ctgcgtttgc atgtatccta ggaatggtgc caaaagtaga ttatactgct
     1381 gatcctaaag catggtggtt ccaattattc acgaatatca tcacaccaat tgtcttgatc
     1441 ttattaggta tgatcttgcc agcaattgct cgtcgagaca aaaacaaaga aattactgaa
     1501 ccagaaacag tggttcagcc ttaaaataga aatacttgtt acaagaggtt gtgaca
//
LOCUS       DASNNU010000197         1537 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.204, whole genome shotgun sequence.
ACCESSION   DASNNU010000197 DASNNU010000000
VERSION     DASNNU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.204"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            98..391
                     /locus_tag="U8116_002783"
     CDS             98..391
                     /locus_tag="U8116_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435704.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            388..612
                     /locus_tag="U8116_002784"
     CDS             388..612
                     /locus_tag="U8116_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN8435705.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            609..>1537
                     /locus_tag="U8116_002785"
     CDS             609..>1537
                     /locus_tag="U8116_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN8435706.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYV"
BASE COUNT          526 a          245 c          319 g          447 t
ORIGIN      
        1 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
       61 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
      121 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
      181 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
      241 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
      301 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
      361 taaaacttta tttagggaga gaagaaaatg aataataaaa cgttcgaagt actaaaatgg
      421 ttcgcactgg taattattcc cgcactagct actttcgtgg ggttagttgg taaagcgctc
      481 aattggcagt acacagatat ctgtgttgtc atcattactg gttttggcgc gtttttaggg
      541 agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct cggctgaaag cgaagaagga
      601 ggaaacaaat gaaaaagaaa attactatta ctgcgatgag cctgttaacg gctctttttt
      661 tattacccat taatacgttt gcttatacta ttaatgacga gtataattta gcgccgaatc
      721 aaggagactc cagattagca attcctaaca aaattatttt gcacgaaact ggaatagatg
      781 caccagcaag aaacgtagcc gccaacatga aaaataatta taacggaagc aattcttata
      841 ctacagatgt tattggtgac ggtgggattg tttaccgtgt gggtgagcaa ggatatgttt
      901 cgtggggagc tggtaacgct aatccttatg cgcctgtaca gattgaatta cagcgcacat
      961 atgataaagc attgtttgaa aaaaactatc gagcttatat tgaatataca agagatagtg
     1021 caaaaaaata tggaattcca ttgactcttg atcaaggaac ttctttattt acaaaaggaa
     1081 tcatttctca tttgtgggtg acaaattatg tttgggggaa ccacacagat ccatatggtt
     1141 acttatcgca aatgggagtt agcaaagaaa agcttgctta tgatttagct catggattta
     1201 ccgatgaaaa tccaacaact tctgaaaaca agcctgtcat tgatccaaca cgagctggtg
     1261 cagctaatcc tacgctgaca gatggaacaa atcacgccca cattgatcag tttggggaaa
     1321 tcgaaaacgc aaacttgcat gtggctggat ggcacattgc taactataaa tacgagtata
     1381 ttttcattat ggactacaat actgggaaag aattagctcg agtaagagct gatggaattt
     1441 atagaccaga tgtaaaccaa gcttataata cttctggaaa tgttggttat catgtatctt
     1501 tcaatatgcg taattttcct agtaagaaag tctatgt
//
LOCUS       DASNNU010000198         1536 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.205, whole genome shotgun sequence.
ACCESSION   DASNNU010000198 DASNNU010000000
VERSION     DASNNU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.205"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="U8116_002786"
     rRNA            complement(<1..>1536)
                     /locus_tag="U8116_002786"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DASNNU010000199         1520 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.206, whole genome shotgun sequence.
ACCESSION   DASNNU010000199 DASNNU010000000
VERSION     DASNNU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.206"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            114..1514
                     /locus_tag="U8116_002787"
     CDS             114..1514
                     /locus_tag="U8116_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435707.1"
                     /translation="MWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIY
                     YRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIA
                     CMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTD
                     LQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTW
                     VCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGA
                     TVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERP
                     HLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVND
                     SYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKD
                     SRYVTKKEINKKNENK"
BASE COUNT          530 a          217 c          262 g          511 t
ORIGIN      
        1 catttaacaa ttatggagat ggactgaact atttttatcc ttttcttaca ataattcctg
       61 cagtcgtttt ttatggaatt tcaaataact taatattaag ttatgttcta tatatttggt
      121 tattaaatat ttgtacgatt ctgattagtt tttattatgg agaaaagttt tttaaaaaga
      181 tagatgctgc ttttttattt agttgtttat atacttttgc aacttatcga acagttgata
      241 tctattatcg atccgcaatt gcagaagcaa tcgctcttac gattatccca gctgttttat
      301 attatgctta tggattgata ttcaatagag aaaaaaaagt catcgcttta tctgtcagta
      361 taagtctact tgtgtatact catgtcttaa ctacactatt gtgtagtgca ttgattgctt
      421 gtatgattat agttcgtttg tttttaagca agcaaataaa aaacgatata aaatttgtgg
      481 tcgtgaattt agtaaaggct gtttttttga cttgtgcatt aactagctat ttttggtatc
      541 ctatgttgca gcagatgaat tatcaagaaa tacacagtcc cttcaaaaca gatttgcagc
      601 tagaagcact ttctatttca gatagtatca ttcaagcgtt aaataacgat attacaacat
      661 acactatggg gttattagga ttggttgcgt tgatttttcc tttattcata ctgaaagaaa
      721 tggagaaaaa agaaaaaatc atctatgttg gcgtagtttc tacatgggta tgcgtaacta
      781 atttatttcc atggtttttg tttcaaaaaa caccgataag cttactccag tttccttggc
      841 gtatcttagg tatccaaatc tttttttctt caatgatcat aacgcttgtt tttatgaaaa
      901 agactatgaa taaaaaatat tcatggttaa tcattggcgc tactgtggtg tttctactcg
      961 tcacgtctat agcagcaaaa gaaaattacg caaaagttat ccaatcaaaa cacggtcata
     1021 ttgttatcaa tgaagaaaca ctcccacgtt atacaacatc agatatggga ggattatacg
     1081 attacgcccc aacagaagcg ataaaagaaa ggcctcactt agaaaaacat gaagtaaaag
     1141 taggagaatc gtgggaaaaa gggaagtata aagctgcaga taataacatt acttacacag
     1201 tggcaagcaa caaaaagcaa aaagtgacag taccagtata tgcttattta ggtacacagg
     1261 taaaagtaaa cggtaaagtc gtaaatgact cttataaaaa acatggctta gtgaatgtgg
     1321 atgtaaatgc tggagaaaat aagatagaaa ttacaacaaa atatactcca actgctctca
     1381 tggctttatg gatcagtatt ctgacttatt gtttagtggt gtattcaagt tttaaacgat
     1441 ttgcacctaa taagttgaaa gattctcgat atgtgactaa aaaggagata aacaagaaga
     1501 atgaaaataa ataaaaaagt
//
LOCUS       DASNNU010000200         1515 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.207, whole genome shotgun sequence.
ACCESSION   DASNNU010000200 DASNNU010000000
VERSION     DASNNU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.207"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(824..1354)
                     /locus_tag="U8116_002788"
     CDS             complement(824..1354)
                     /locus_tag="U8116_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HEN8435708.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1351..>1515)
                     /locus_tag="U8116_002789"
     CDS             complement(1351..>1515)
                     /locus_tag="U8116_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN8435709.1"
                     /translation="GKIAFYFSAIIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKL
                     ILITKRDGER"
BASE COUNT          469 a          306 c          173 g          567 t
ORIGIN      
        1 atatctttta cctttctcct gtgaaaagga aatttccttt tttatagtgt tattgatata
       61 atcagaatat tcatcaaact ctgtatctgg agttaaaaat ctacagtaac atctataagc
      121 tttgctagta atcttcttgg gttctctctc cattgtcaag caacatcaaa acacctactt
      181 actattcgat tcttttctgc caaaaacgag tcagcatcac tcacaactta tgtgttgctg
      241 actccgacta gtcctttgtt caatatcaac ttattaaaca gatataatat ttttctttat
      301 ttcgtatact ccaagacttc catctttctt tcatcttatc tctgaaaaaa acacagcaaa
      361 gtgcccttaa taaatataca ttagaatttg caaatttttt catcttaaga tatatcctat
      421 tcatattctt tacaatccta atgggtgatt acaaatctat ttagttcaag ttctacatta
      481 taaacacctt tcttgatagt atcatctgct gcacaagcga tgtcgatcca cttattaggg
      541 gatttacgtg ctcaaaagtg gctattcctt ttgggaagtc catccataat aatggtccat
      601 ggattttttt agggtcttgt cgttcaaatt tctcaaaaac tttattatac ttccatagtt
      661 ttataattat ttggtttcac tttggctcgt tcaaacacca acttgtattt cttttcaacg
      721 ttcgaactgg agccccccct ataatctcat tattattttc atgacttttt actaccgtgg
      781 aattacaagc aacaacaata ttatctgcta aatgaacacc tttctatatc acgctttttg
      841 ctccaatcca acaatgttta cctataacta ttttattatt tttattcaaa atcctatgag
      901 tattcaattc aaaaatcgta tgtgaatcgg tatccataaa taatgtatcc cacgatactg
      961 tacagccatc tttgaattga atttccttaa agcaaattat actcattttt cctgtcgaat
     1021 taaactttga cccaatatct aaaactgcat ttttccttat gtcaatatga gccccttgtg
     1081 cgataaacgc tggcccttgt attcttagta ttccttcaat acttataatc gtttcttcat
     1141 ttcccgcagt atttaatact ggaaagccca aataaactat ttttccagat tgtaatacta
     1201 tctcaatttt ccctctcgat ttgcgtgtaa tccgtacttt acgatgcacc aaaataggta
     1261 atctccacgc atctgaaata tgaagaaatt tataattgaa gtatatcgtt ttaggtaaat
     1321 ttaatatatt ctttattatc aatccgagtc tcatctttca ccatctcttt ttgtaatcaa
     1381 aattagttta taaaggatta cacccaatat accgcccaac gtattatata caaaatccga
     1441 aatttgaaac gttcctagcc tcaaaaaaag ttgtaaaaat tcaattattg ctgaaaaata
     1501 aaatgcaatt tttcc
//
LOCUS       DASNNU010000201         1494 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.208, whole genome shotgun sequence.
ACCESSION   DASNNU010000201 DASNNU010000000
VERSION     DASNNU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.208"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1450
                     /locus_tag="U8116_002790"
     CDS             <1..1450
                     /locus_tag="U8116_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEN8435710.1"
                     /translation="KGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVANGGL
                     GLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLALPFG
                     VSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALLAPLI
                     VGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKGFIRN
                     LIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAEEKPR
                     VWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIFIFIL
                     TPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWLLGSF
                     FIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTPGTES
                     AYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          404 a          287 c          315 g          488 t
ORIGIN      
        1 caagggcttc ttcgggcagc cgaagggatt gtcaactttg ttcttcactg agatgtggga
       61 acgatttagt tattacggta tgcgtgcatt acttatctat tatatgtacg atacagttgc
      121 caacggggga cttggattac cacgagcaac agcattagct atcatgtcga tctacggatc
      181 tttgatctac atgtctagta tcgtcggcgg atgggtatct gaccgagtgt taggagcaaa
      241 acgcactgta ttcttcggtg gagttttcat tatgcttggc cacatcgtct tggctttacc
      301 atttggtgta agtacattgt ttgtttcgat ggcattgatt attatcggaa ccgccttttt
      361 gaaaccaaat gtttctggca tggtcggaca tctttattca aagactgact tgcgaagaga
      421 tgcaggattt tcgatttttt acatgggtgt caacttaggg gcattattag caccactaat
      481 cgttggtaca atcggtcaag aatataacta tcatttaggg ttctctttag ctgctattgg
      541 gatgttcttt ggattattac aatacatcat ccaaggacgc aagacattag acggagtggg
      601 tacaacaccg ccgaatcctt tgacaactga agaacgtaaa ggattcatcc gtaacttgat
      661 catcgcactt gttgttatcg ctgcagtatt cggcggggca caactaactg gtcatctgac
      721 aattgacttt tttgtcaata caatcagtgt attggggatt atcttaccgt tgtattactt
      781 tatcaaaatg ctgacttcaa aagatgtaac tgctgaagaa aaaccaagag tatgggctta
      841 tatcccatta ttccttgcag caatcatttt ctggtcatta caagaacaag gttcatctat
      901 cttagcttta tttgctagtg aacgtacaca atctacgcta tttggtgtaa cgatccaagc
      961 aagctggtat caatcattga atccaatttt catctttatt ctgacaccag tattcgtaac
     1021 gatgtggacg aaacttggtg aacgtcaacc atctacagta gtgaaattct ctcttggact
     1081 tttatttgca ggattatcgt acttgctatt gatgttgccg ggtatgcttt atggtacaag
     1141 tggaaaagta agtccattgt ggttgttggg aagtttcttc atcgtggtta ttgctgaatt
     1201 gtgtctttca ccagttggtt tgtctgtgac aacaaaatta gcaccacgag catttgaatc
     1261 acaaacaatc gctatttggt tcttagctga tgcttcttca caagcaatca atgcacaaat
     1321 tgcacgcttc tacacaccgg gaacagaatc tgcttacttc ggtattatcg gtgcagtagc
     1381 aatcgtcggt ggtatcttgc tatttactgt gaaagaacca atcaaaaaac taatgggctc
     1441 tattcattaa tcaatgaaca taaaaacagg aattgcttat ttgcagcagt tcct
//
LOCUS       DASNNU010000202         1482 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.209, whole genome shotgun sequence.
ACCESSION   DASNNU010000202 DASNNU010000000
VERSION     DASNNU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.209"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..828
                     /locus_tag="U8116_002791"
     CDS             <1..828
                     /locus_tag="U8116_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEN8435711.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETKIVDA
                     KKGKINYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFERWRQEQEHSFQDWREGQESDYLAWF
                     ESIKDILKTIDPGGVMLAELTDARVDIQGVRHASISERLLADMDYLYQKLRATLFTIE
                     YGEIEVTDILQDDLFSDNHEVEKVGTVAFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1448
                     /locus_tag="U8116_002792"
     CDS             828..1448
                     /locus_tag="U8116_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435712.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          529 a          212 c          337 g          404 t
ORIGIN      
        1 aatacagagg ttatggaagc gactatagtt gaaaatggtc ttccctacga tttatcagaa
       61 tgtaaggtat attttgaatc agttgtaggt gggaaatatc cagtccaatt agaaacaaaa
      121 attgtggatg ctaaaaaagg gaaaattaac tatatttttg ataaatattc catgcagtgt
      181 ttacatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cttattttgt cattaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caatcgaaga tttaattgcg gatatgacgg actttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa
      421 caggaacaca gtttccaaga ttggagagaa ggacaagaat cagactattt ggcatggttt
      481 gaatcaatca aggatatttt aaagacgatt gatccaggtg gagtcatgtt agccgaacta
      541 acggatgcgc gagtagatat tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg
      601 gcagatatgg actatttata tcagaaattg cgagcaacgc ttttcacgat tgaatacggg
      661 gagattgaag tgactgatat tttgcaggat gatctctttt cagataatca tgaagtcgaa
      721 aaagttggaa ctgtagcatt cccgattgaa gaaggagcct tgatcatcgc aaccgttgat
      781 gatccaaagc aaaatgtgtt cactcttgag aaagttgggg tgatctaatg gctaaaacta
      841 aacgaatgat ggaaacggat gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac
      901 atgcttccgc agttcttggg ttagaagaaa taatagcagg agaagcaaca gttttatctg
      961 ttaatggaaa aattggagcc gtcgtcatta ctaaagaaga tttgggatta gagaatgttc
     1021 tcacagaatt accctatgca agtgaagaag atgacggcat tatcacggca gaaatgtatc
     1081 aaaaaatttt aaacagtgga gaaggtgact acgtgttgcc agttgccact atcgaccgtt
     1141 tgggtggcat aaaagttggt gaattattga cgattgatga gacagggaaa gtctctgcag
     1201 tcagacaatc tgatgtcaat ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa
     1261 attatactgc tggagaaaac atcactattg atgaagatgg aacaattagt tctacaggtg
     1321 gtagtgggac tggtggtgtc aatcagtcat atgtagatca aaaattccaa gaagctgtaa
     1381 atcaagcaga aaattacaca aacgaaagaa ttccaaattt tacttttgaa aaaattgggg
     1441 aggtatagag aatgacagat atcgtaaaaa taaaacaaag ca
//
LOCUS       DASNNU010000203         1479 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.210, whole genome shotgun sequence.
ACCESSION   DASNNU010000203 DASNNU010000000
VERSION     DASNNU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.210"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..828
                     /locus_tag="U8116_002793"
     CDS             <1..828
                     /locus_tag="U8116_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEN8435713.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETRIVDA
                     KKGKIKYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFDRWREEQENSFQDWREGQESDYLSWF
                     ESIKDILKSIDPGGVMLAELMDARVDLQGVRHESISERFLSDLNYLYQKMKAALFTIE
                     YGEIEVTDILQDDLFSDNHEVEKIETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1445
                     /locus_tag="U8116_002794"
     CDS             828..1445
                     /locus_tag="U8116_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435714.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          544 a          192 c          311 g          432 t
ORIGIN      
        1 aacactgaag ttatggaagc tacaattgtc gaaaatggtc ttccatatga tttatcagaa
       61 tgtaaggtat attttgaatc agttgttggc ggaaaatatc cagtccaatt agaaacaaga
      121 attgtggatg ctaaaaaagg gaaaatcaaa tatatttttg ataaatattc catgcagtgt
      181 ttgcatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cctattttgt catcaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caattgaaga tttaattgct gatatgacag attttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgatcgctg gagagaagaa
      421 caggagaata gtttccaaga ttggagagaa ggacaagaat cagattattt gtcatggttt
      481 gaatcaatca aggatatttt gaagtctatc gatccaggtg gagtaatgtt agccgaatta
      541 atggatgcac gtgtagatct tcaaggtgta cgccatgaat cgatttctga acgtttccta
      601 tccgatctaa attatttgta tcaaaaaatg aaagcagcac ttttcacgat tgaatacggt
      661 gaaattgaag tgactgatat tcttcaagac gatctctttt cagataatca cgaagtcgaa
      721 aaaattgaaa cagtagaatt tccaattgaa gaaggagctt tgattattgc aactgttgat
      781 gatccaaaac agaatgtttt cactcttgag aaagttgggg tgatttgatg gttaaagtaa
      841 agagaatgat ggagactgat gaatctggtg ttaaacgtca atttatgcca attacacatg
      901 tttctgcagt tttaggctta gaaaaaatga tatcaggaca atcaaaagtt ctttcggtta
      961 atggtaaata tggtgcagtc attttaacga aagctgattt aggattagag aatgcaatta
     1021 ctgaactacc ttatgcaagc gagacaagcg atggtattct tactgctgaa atgtttcaaa
     1081 agatcgtaaa tggagaaggt ggaacatata ttttgccgat tgctactcct gaacaattag
     1141 gaggtataaa agttggggaa ctcttagaag ttactgaaga aggtgtttta tctgcaacta
     1201 agcaaacaga cttcaatttc tctgaagaat taaagggaaa attagaatct ctaaaaatat
     1261 taaaagcagg tgcaaatata tcaattgcag aagatggaac aattagttct acaggtggta
     1321 gtgggactgg tggtgttaat cagtcatatg tagatcaaaa attccaagaa gctgtaaatc
     1381 aagcagaaaa ttacacaaac gaaagaattc caaattttac ttttgaaaaa atcggggagg
     1441 tatagaaaat gacagatatc gtaaaaataa aacaaagcg
//
LOCUS       DASNNU010000204         1422 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.211, whole genome shotgun sequence.
ACCESSION   DASNNU010000204 DASNNU010000000
VERSION     DASNNU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.211"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..299
                     /locus_tag="U8116_002795"
     CDS             <1..299
                     /locus_tag="U8116_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEN8435715.1"
                     /translation="TKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFGYEKQ
                     IPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN"
     gene            336..1346
                     /locus_tag="U8116_002796"
     CDS             336..1346
                     /locus_tag="U8116_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEN8435716.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT          495 a          230 c          318 g          379 t
ORIGIN      
        1 ctacgaaaca aacggatttc aaagcattag ggaagatcgt cggtaaaagg ctttctattg
       61 tttcatcaga acgcatggaa gaacaaacgg gacaaaagca gggcgctgtt tctccgtttg
      121 gttatgaaaa acagattccg attattattg atgaagagct cttatcaaat gaaaaacttg
      181 tttttgcacc aggtagacca gatcaaacga tggtcatcca aacggttgac ttaaacaaaa
      241 tcatccagct acttgagatg ccgatatttt tgatgccggc aaacgaaaaa aacaattaac
      301 caagtaaaat agtgcatact tggaggtgaa gcaacatgat aagttatcca ttattagaaa
      361 gaaatgcaac gattggggtt actgcaccat cctcaggcgt atcagaggaa caccattcgt
      421 tactcgaatc agctttaaag agaatggaag aaaaatcttt ccaagtagtt actggaaaga
      481 cgctttggac gcaagataag gaaaaatcag gtccagcaag aaaacgagca gatgaattca
      541 atcaaatgat gcaagatgaa aatatccaaa tgatctttcc gacttgggga ggagaattat
      601 tgattgagat tctcgatttt gtcgattttg atgctctaca accaaaatgg atagtaggtt
      661 attcagatat tagtacgttg ctacttgctg tcacattaaa aacagggata gccactgcac
      721 atggaactaa ccttattgat ttaagaggag agtacttgga tgaaacgaca gcaatgtggg
      781 aaagtgtact gaaaacaaga gaaggagaat cgattctaca gcaatcttct gaaaagtatc
      841 aaaaagtgtg gccccatgag cattcatcat cttctgtttt ccatctcact gaaccaacaa
      901 attggcaatc agtcagcgga gatgcagtaa ggatgaaggg acgattatta ggtggttgta
      961 ttgatacgat caggcattta atcggtactc cttatggaga tgtccaatct tttaaaagaa
     1021 agtataatga tggaaagccg atcttgtggt ttctagaaaa ctgtgagcta tcgtcagttg
     1081 atttaagaag atcacttgtt cagatgaaac tggcaggctg gtttgatggc agctcgggga
     1141 tcatgttcgg tagaagtgcg gcagaggatt tgttagatta taaaatgaag gatgtatacg
     1201 aagatctgtc aaaggaatta aaactaccga tcttgtatga tattgattgt ggtcatgtac
     1261 cgccacaact gacattgatt aatggtgcct atgcagaagt agcggtagaa gatagaaaag
     1321 cgactgtttt gcaaacgttt atttaaaaaa ttttttcctg ccttggagtc tatcaaagac
     1381 caataaatct aaatagaaaa gaaagtgaaa gaagctgaca aa
//
LOCUS       DASNNU010000205         1417 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.212, whole genome shotgun sequence.
ACCESSION   DASNNU010000205 DASNNU010000000
VERSION     DASNNU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.212"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(150..1337)
                     /locus_tag="U8116_002797"
     CDS             complement(150..1337)
                     /locus_tag="U8116_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEN8435717.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DASNNU010000206         1401 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.213, whole genome shotgun sequence.
ACCESSION   DASNNU010000206 DASNNU010000000
VERSION     DASNNU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.213"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            316..570
                     /locus_tag="U8116_002798"
     CDS             316..570
                     /locus_tag="U8116_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN8435718.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="U8116_002799"
     CDS             571..888
                     /locus_tag="U8116_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN8435719.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          488 a          222 c          274 g          417 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag g
//
LOCUS       DASNNU010000207         1397 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.214, whole genome shotgun sequence.
ACCESSION   DASNNU010000207 DASNNU010000000
VERSION     DASNNU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.214"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(298..912)
                     /locus_tag="U8116_002800"
     CDS             complement(298..912)
                     /locus_tag="U8116_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN8435720.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          464 a          271 c          229 g          433 t
ORIGIN      
        1 tattaagaga caagaaagaa tccatatgga ttctttcttg tctctttttc cactactttt
       61 tatttttctg acactacata ttcttataac tcatttctca taactgtgaa acaacttata
      121 aaaataacaa actttaaaca aaacaagctt aaaatcaaag atgatccatc ttaaaataac
      181 taaaacaaaa aaagtagtcg ttgttaatcc tcattaataa tcgttttctt tttttcaaac
      241 gctccatttt tatgtggaac attatttttt taaggaactt tgtcaacatg tggataacta
      301 ttttccactt gtgtcctgga ctagctgttt tgctgtaaaa tacaaaactt gctgcagcaa
      361 aaagatcgtt gcaagagagg ctgttccgca gatcgctgga tctagatttg gcaaagtcaa
      421 gactgcatca tgtaaattgg cgtggttgtc caaaaccaaa tcagcaaatt caaataatcg
      481 caaaccactg ctatgttgcg atttgatact tcgtgacaaa tcaaatccag taacaatgat
      541 taatggatga ccattttctt tgatccactg agctagttcg atgattgccg ggtttcttcc
      601 ttcattcgaa agaagaagaa aaatgtcttc tgatttgaca gaaagctgat caataattgc
      661 ctgtcctgca ccttctagcg tttcatagac acctccggct ggattttcaa tcaatatact
      721 tggtacaaga tccggggttt gatcccaaaa ttcaaatgct acaccactta gtgtgcggct
      781 agcatatatc tgcaatctac caccttgatt aatttgtttc gcgatccaag tgccagcttg
      841 aagcatcagc ggctcttcta tccttaattt ctcttccaat tggtgataat agtcaaaata
      901 acataaactc atttatctct cccgcctagc tatacttgac atgaatagaa acagctgcct
      961 aatctaagga agtacataaa aaaacagtag ttagacagta acacatgtgg tctttgctgt
     1021 ttcaaacaca aaaaaggcat tgaacgaata cgcccaatac cttgacatag caaataaaac
     1081 aacgtgcaga ccatggaaaa tatctacata ggggtagaat tttcacccta gattaggtac
     1141 aataaattag gtcgatgcac tgcattgact atggcaaaat gatcggtcag ggtgtatgtg
     1201 tgtgactggt cattccctga ttcttagcgc caactaaggg tcagggagcc tttttttatt
     1261 cagctgtttc tatctgaatt tattgtaagt aatctcttat ccaagtgcaa gcaagaaaat
     1321 acaaaaaaac attggtatga tttatatata tataaataac ataaaaaaag gaatggctta
     1381 tcccgataca acaagca
//
LOCUS       DASNNU010000208         1387 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.215, whole genome shotgun sequence.
ACCESSION   DASNNU010000208 DASNNU010000000
VERSION     DASNNU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.215"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..331
                     /locus_tag="U8116_002801"
     CDS             <1..331
                     /locus_tag="U8116_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HEN8435721.1"
                     /translation="YGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            282..>1387
                     /locus_tag="U8116_002802"
     CDS             282..>1387
                     /locus_tag="U8116_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEN8435722.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGL"
BASE COUNT          472 a          200 c          293 g          422 t
ORIGIN      
        1 ttatggaact gttatttttg cgaatgctgt aattaaccca gatgctgtaa ttggagagca
       61 tgctataata aacacaggta gcattgttga gcatgattgt agaattaata actatgttca
      121 tttatctcca ggtgtatgct tagcgggtgg agtacatgta ggtgtaggaa ctcaaatagg
      181 aattggttct caatgtatcc agatgaagaa aattggatct tggtgtatga taggcgcagg
      241 gagcactatt gttaaggata ttccctcaca ttccttagct tatggcaatc cagctaaaat
      301 taagaaagaa ggaattaatt ttgaattcta gaattttatt atcaagtcct catatgggag
      361 gaacagaaga aaaatatgtt gctaaggctt ttaaagataa ttggatagcg ccattaggtg
      421 aaaatgtgga taaatttgaa gaaagtatta caaaatatac tggttctaag ggcggagctt
      481 ctgcgttaag ttcaggtaca gctgccatac atcttgcctt aagactgctt aatgttggaa
      541 aaaacgatat tgtctttgct ccttcattta cttttatagc tacagtaaat cctattttat
      601 atcaaggggc tgtaccagtg ttcattgact cagaacctaa tacatggaat atgagccctg
      661 ttgcactaga gagcgcatta gaagaagctg aaagaaacaa ccaattacct aaggcaatca
      721 tcgtagtgaa tttatatggt caaatggctg attatgaacg aattttagct attgcaaacc
      781 aatttaatgt tccagtgata gaagatgcag cggaatcgtt aggttctact tataagggaa
      841 tttcgtcagg cttatttggg acaattggga tctattcatt taacggtaat aagatcatca
      901 caacctcagg tggaggagct ctagtgtcta gtgataaaaa atatgttgag cattcaagat
      961 ttttagcaac acaagcaaga gatcaagcac cgtattatca acatagcgaa ataggttata
     1021 attaccgtct aagcaatatt tctgccggta tcggacgtgg acaaatggaa gtattgaatg
     1081 atagagtagc taaacgaaga gaaattttca atcgatataa ggaagctttt tctggaattg
     1141 atagaattga aatgatgcca gaagtggatg gcatggtgcc aaatcgttgg ttgtctactt
     1201 taacgatcaa tgattataat tctattgatc aagtagttaa cgtaataaat aaactaaatg
     1261 aaaaaaacat tgaaacaaga gctttgtgga aaccactaca tttgcaacca gtatttcaag
     1321 gggtaaaaat gtattaccat gataataatc ttagtgtgtc agaaaagtta tttaatagcg
     1381 gcttatg
//
LOCUS       DASNNU010000209         1363 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.216, whole genome shotgun sequence.
ACCESSION   DASNNU010000209 DASNNU010000000
VERSION     DASNNU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.216"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            74..1236
                     /locus_tag="U8116_002803"
     CDS             join(74..350,350..1236)
                     /locus_tag="U8116_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN8435723.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANK
                     HKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAK
                     RGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIV
                     TDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFH
                     TDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLA
                     QLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          461 a          261 c          295 g          346 t
ORIGIN      
        1 gaatcggcta cactagacta gacagaaaaa ataaggtgtg tagaataata aaaaacacac
       61 tggaggattt ttcatgtcaa gacgtcaacg aagaacctat tcaaaagaat tcaaacaaca
      121 aatcgtcgat ctctatctcg ctggtaagcc tcgcgcagaa attattcgag agtatgagct
      181 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
      241 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      301 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      361 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaaaat tctaaatatt
      421 tctcgtcaaa cctactatta tcaagcgaaa ccgatcgaaa atgagtccga cttagaagag
      481 atcgttcagg aagagtttat tcgaaaccga aaggcttacg gtacccgaaa attgaagaag
      541 tgtttagcaa agcgtgggct tcaactcagt cggcgccgaa tcggtcgaat catgaaacgc
      601 cgcggattga catctaccta tacgatcgct cattttaaag ggcaacgaac agcttgtaat
      661 gaagcgaaaa cagcgaatgt attagatcgg acctttacac aagaacagcc attggaagcc
      721 atcgttacgg atcttactta tgttcgcgtg gggaaaaagt ggcattatat ctgcttaata
      781 cttgatttgt ttaatcgaga aattattggt tattcctgtg gtgagaagaa agatgcctca
      841 ttggtaaaag aagcctttgg acggataccg tattctttaa cagacgtcaa gctttttcat
      901 acagaccggg gaaaggaatt tgataaccaa accattcatg agattctgaa tggttttgga
      961 attactcgtt cattgagtag gaagggttgt ccgtatgata atgccgttgt ggaatcaacc
     1021 tataaatctg tcaaagtaga attcgtgcat caataccaat ttgagacact ggcacagcta
     1081 cgtctagaat tgtttgatta tgtgcattgg tggaactatc tacgcttaca tggcacgttg
     1141 gcgtatgaaa caccgatcca aattcgacaa cagagattgg cgaagcgaat ccttgataat
     1201 gagcgcggat ctgatacctc tggagaggca gcgtaattga atgattgtgc ttctgccgga
     1261 gaaaatcaga tccgaggatg ctcattgtca agggcaatcg tagccataac accgcagcat
     1321 tcacaacacc ttataatttt tgtcaaaaaa agtgttgcca ttc
//
LOCUS       DASNNU010000210         1362 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.217, whole genome shotgun sequence.
ACCESSION   DASNNU010000210 DASNNU010000000
VERSION     DASNNU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.217"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>1362
                     /locus_tag="U8116_002804"
     CDS             82..>1362
                     /locus_tag="U8116_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEN8435724.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DASNNU010000211         1362 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.218, whole genome shotgun sequence.
ACCESSION   DASNNU010000211 DASNNU010000000
VERSION     DASNNU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.218"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            88..1257
                     /locus_tag="U8116_002805"
     CDS             88..1257
                     /locus_tag="U8116_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN8435725.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          499 a          201 c          218 g          444 t
ORIGIN      
        1 taaatagata tttagggtag aaaaaaagaa agtccattct gtaaaattaa agttaccaca
       61 accgaaattt tataggagga ctttctcatg cccaatttta ctacagaaat tatggaaaca
      121 ctaatcaata aaggtgattt agatgaatta tttcgatgtc atttagaatt agctgttaat
      181 tcattattgc aggctgaatt aacagctttt cttgattatg aaaaatatga tcgtgctggg
      241 ttcaattcag gtaattcccg taatgggaat tattcacgtt cattcagaac agagtatgga
      301 gaattaaatt tggtaattcc tagagataga aatggtaagt tttctcaaca aactttacct
      361 gcttataaaa gaaccaatga ctctttagaa accactatta ttcagctatt tcaaaaagga
      421 attactatgt ctgaaatctc tgaattgatt gaaaaaatgt acgggcatca ctatacgcca
      481 caaaccattt caaatatgac taaaatagtt tctgaagata ttattgcctt taaagaaagg
      541 tccttagaat ccagatactc tgtcattttt atggatgcta ctcacattcc tttaaaaaga
      601 caaactgttt caaaagaagc agtttatatt gtaattggca ttcgattaga tggaaccaaa
      661 gaggttcttg ggtttagtct tgctccaact gaatctgctt atgtttggaa agagattctt
      721 caagacctaa aagaccgtgg tttaaaagag gttttattag tcgtaactga tggtttaagt
      781 ggcattaacg atagtattca tagtgtttat ccaaatgctc aatttcaaca atgttgtgtc
      841 catatatcta gaaatatcgc tcataaggtt cgtgttagtg atcgacaaga aatctgtagc
      901 gatttcaaat tagtttatca agcaagttca aaagaagaag caaataatca aataaggttt
      961 atgatagata aatggaaaaa gcagtatcca cgagtggtta aattgctcat gaatcctgct
     1021 atattaacct tttataattt ccctccgtca atcagaagaa ctatttattc aactaatttg
     1081 attgaaggtt ttaacaaaca attaaagaaa tataccaaaa gaaaagagca atttcctaat
     1141 gaagaatctc tggagagatt tctagtatct caattcaaca actataatca aaagtttcta
     1201 tgccgagttc ataaaggttt taaagaaata catgatacat tagaatcaat gttttaactt
     1261 gatcaacaga atgggctttt ccatttacac aaaattattg acgctcccga caaaattatg
     1321 atcttgcgta aagttataaa atattaataa aaatatttta tt
//
LOCUS       DASNNU010000212         1360 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.219, whole genome shotgun sequence.
ACCESSION   DASNNU010000212 DASNNU010000000
VERSION     DASNNU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.219"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            73..>1360
                     /locus_tag="U8116_002806"
     CDS             73..>1360
                     /locus_tag="U8116_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN8435726.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHSKTFL"
BASE COUNT          432 a          268 c          258 g          402 t
ORIGIN      
        1 aaaaaaatat agaaaagaca ccctgaaatc ctttagaatt aatttggcta ccaattcata
       61 ggaggattca agatgtctca tactcagctt atcaaagata ccctaaatat tctagaccta
      121 aatatctatt tcgaggaaaa ttgtttaact aaagaaaaat acaaagggca aatttgcttg
      181 atttacaaag gaactcttct gtacaagcca gaagagtgtt cccattgtct ttgtgtcgtc
      241 ccctctagga tcattcgttg ggggacgaca accgttcggt tgttactgaa tgatgtgtct
      301 gagtatcgca cttatctgga gctgaagaaa caacgattta aatgtaagtc ttgccaaaga
      361 acattcgtag cggatacctc tgttgctgaa aaacactgtt tcattagtca aaaagttcgt
      421 tggtctgtca ttgctcgctt aaaagagaat acttccatga cggaaatcgc tcgtcagaaa
      481 aatatttcca cttcttcggt ctatcgtgtc atgaagcgat tctaccgccc attgaatcct
      541 ttcaaacaga ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt
      601 gccagtgcaa tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc
      661 gaaaatcgac gcttgcctta tttagaacgc tatttttctc ggttttcatt agcgactcgt
      721 gaagccgttc aattgattgt tattgatatg tatgcgcctt atgtttcgtt ggtgaaaaag
      781 cttttcccga atgctcagtt gatcatcgat cgtttccaca tcgtccaaca tattggcaga
      841 actttcttga atcatcgagt gaaagaaacc acggtacgac ttaaaggaaa ttcccactct
      901 caacgagggt tagggaaaaa attgaagcgc tattggaaac tcctacaaaa agaagaagca
      961 aagttaaact atgaaaaacg ggtttggcgt gccagtttta aagattattt gactgaaagt
     1021 gagattgccg atcgactgtt agctgcttgt cccaacttac gccaaggcta tcagttatac
     1081 caagacattc tttatgcggt aaagaaaaga gatcagtcac tatttgagga ttgtttaact
     1141 agagacatta gcggtctgcc agaaacctat acaaccactt tacgaacgtt taaaaagttt
     1201 ctgccacaga tccaaaatgc gcttcactac agctattcaa acggaccatt ggaatgctta
     1261 aacaaccaca tcaaagtatt aaaacgcaat gcgtacggct ttcgcaactt ttacaatttc
     1321 aaactacgaa ttatgattcg tcatagcaaa acatttttaa
//
LOCUS       DASNNU010000213         1356 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.220, whole genome shotgun sequence.
ACCESSION   DASNNU010000213 DASNNU010000000
VERSION     DASNNU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.220"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(132..1280)
                     /locus_tag="U8116_002807"
     CDS             complement(132..1280)
                     /locus_tag="U8116_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN8435727.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          389 a          240 c          216 g          511 t
ORIGIN      
        1 acggttctta acgcttgaag tgggagcttc ttgggaatag agcatacttg atagcgtatt
       61 tctttactaa cagcggtgtc tcttatttac caagctatcc ccgtagttcc tacggttctt
      121 gatgattgga tctaagccaa tcccatagaa tgtagtctta gaccttcggt cagaatattg
      181 atactagcat tgatatcacg atcatgatga gtatgacaaa caggacaagt ccattctcga
      241 atttcaagag atttcttgcc atctttatgt ccgcactctg aacaaatctg actagacgga
      301 aaccatttat ctactttgat gatttttcgt ccataccagt cagccttgta ttgtaattta
      361 gtcacaaact ttgaccaaga aacatcagaa atacttttag ctaatttatg atttcgcaac
      421 ataccttttg tgtttaagtc ttcaatacag ataatatcgt gatttttgat catttctgta
      481 ctcaacttat tcagaaaatc agtacgttga ttcattactt tttcatgcaa tctagctact
      541 ttgcgttttt gtttttgata atttttagct tcaaatagat tggtaccttt atttttagcc
      601 aataaagcac gcctagacaa tttgcgttgt tcacgcttta gtttcttttc cattttggac
      661 gtgaatttat tattatcaat tttttgtcca tcagaaagaa tcgcaaagtc cataatacct
      721 aagtcaatac caattgtgga attagtttta ggtaattcga taatttcttc tttacacaac
      781 aaagaaatat agtatttacc gctagaatga cgtgatattg tagcggattt gataatccct
      841 tttggttgtc tatgaagctt aattcttact aatgacttca atttaggaac tttgatgaat
      901 ttactatcaa tcaaagcaac tgtaccattt tgattattcg ttgtataact ctgaacagga
      961 tttttcttac ttttgaaacg tggaaatcca acggatttat cccgaaaaaa attcttgtat
     1021 gctttatcta aattaagttg ggcattggct aaagcaaggc tatcaatttc tttcaaaaac
     1081 ggaaattctt tcttgtattt agctggtgtc ggaaaagtca tttttttaga agaatcattt
     1141 ttaacttctt catacgcctt ttttcggtca tcaagcatta gattgtagac cttacggaca
     1201 caaccaaaag atttggcaaa gaagatttct tgttcctctg ttggatagat tctgaatttg
     1261 tatgctttta gttgttccat aaagttcacc tactttctat ttatgattgt ttccttgatt
     1321 ttggatatac ttcttaatca catcaattgg tgcgcc
//
LOCUS       DASNNU010000214         1349 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.221, whole genome shotgun sequence.
ACCESSION   DASNNU010000214 DASNNU010000000
VERSION     DASNNU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.221"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1092
                     /locus_tag="U8116_002808"
     CDS             <1..1092
                     /locus_tag="U8116_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN8435728.1"
                     /translation="FSTGTILGWQFYNNEHSQIVAHAQSAQKKLILTTNEQQLVEKID
                     QLLRKNDYIGSIYVKKNNRIIMEKGYGYANKLTQQPNDPSLYYQIGSVQKALTTLLIL
                     KQVERGHLSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLHRTASPTIPMTDEEVVQFA
                     IQHLKFTDYQTSHYDSLNFTILTGILIKLTNQPYEKLLTDDIIEPLGLQHTKFYDQVK
                     NSSNHALSYQMSKEDDYFKPLTESETEIHNELGTGNISMSVYDLNTLFTQVLSGKLVS
                     KDLLFSLWRENANGHPYSGGVYSGDNYILSQGNIDSFDTSAIFSKDSQDAIIMESNIK
                     ADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          478 a          252 c          217 g          402 t
ORIGIN      
        1 ttctctacag gaactattct tggatggcaa ttttacaata atgaacactc tcagatagtt
       61 gcccatgctc aatcagctca aaaaaagttg atactgacta ccaatgaaca acagctcgtt
      121 gaaaaaatcg atcaactatt aagaaaaaat gattatattg gctcaattta cgtaaagaaa
      181 aataatcgca tcatcatgga aaaaggctat ggctatgcga ataaactcac gcaacaacca
      241 aatgatcctt ctctttatta tcagattggt tcggttcaaa aagcattgac tactcttttg
      301 attttaaaac aagtcgagcg tgggcacctt tctttagata ctaagctggc tcaattctat
      361 ccacaaattg ctggcagcca gcaaattact atccgtgact tgctgtatat gcgctcaggc
      421 ttgcaccgaa ctgcttctcc aaccataccg atgactgatg aagaagtcgt tcaatttgcg
      481 attcagcact taaaatttac agattatcag acctctcatt atgattcatt gaactttacc
      541 atattgactg gaattttgat caaattaaca aatcaaccgt atgaaaaatt attgaccgat
      601 gatatcatcg aacctttagg attacagcat acaaaatttt atgaccaagt caaaaattca
      661 tccaatcatg cactatccta tcaaatgtct aaagaggatg attactttaa accactgaca
      721 gaatctgaaa cagagatcca taacgaatta gggactggta atatcagtat gtccgtctat
      781 gatttaaata cgctgtttac acaagtttta tctggaaaac tagtttccaa agacttactc
      841 ttctcattat ggcgtgaaaa tgcgaatgga catccatata gtggtggggt atatagcgga
      901 gataattata tcctgtcgca aggaaatatc gactcttttg atacatctgc tatatttagc
      961 aaagattcac aggatgctat catcatggaa agcaatataa aagctgacaa aactatcaaa
     1021 ctgcctactg ctgatctaag aaaccaaatc tatgacctga tggaaggaac tactaagttg
     1081 gcaaatagct gatatgatga aaagatagca aatactactt ctttgaatag catttgctta
     1141 aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca aatctaaaaa tcaatttttg
     1201 ctacagactt taattccaaa taaacagaac agaaagaggt gttcttcttt ctgttctgtc
     1261 caaattattc taagatttct ctttaccaac attatttgac aaagggccta ttaaatctaa
     1321 aatagtatct ttagattgct aaaatatgt
//
LOCUS       DASNNU010000215         1312 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.223, whole genome shotgun sequence.
ACCESSION   DASNNU010000215 DASNNU010000000
VERSION     DASNNU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.223"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1312
                     /locus_tag="U8116_002809"
     CDS             <1..>1312
                     /locus_tag="U8116_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN8435729.1"
                     /translation="PMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNGSR
                     LSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYRDY
                     PVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQGI
                     QSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTRLQ
                     VGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKDRP
                     VLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVNPK
                     RLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRNQY
                     VLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSH"
BASE COUNT          466 a          183 c          259 g          404 t
ORIGIN      
        1 ggcctatgac aacctataga aatgaaaacg atttgctata ttcgttagag cacttaaaac
       61 aagaatttcc tgaatatggt acaacagatt ttagacatcc tgctatttca ttactacaga
      121 aaaatggctc tcgcttatca gattttgtct atcaagggta cgaagtggaa gaaggaaaac
      181 ctaaactaga aggtttacct gctacctatg ttgataataa tgatgaaagc aaaacattga
      241 aagtttatat tttagattca gttacaaatg ttcaagtaga attattatat acaatttata
      301 gagattatcc tgttattact cgtagcgcgc gagttatgaa taaaggaaaa gatattcaaa
      361 taatcgagaa tattagcagt ttatcattgg atttacccga tgcaaattat gaatggcaac
      421 aattatctgg tgcttggggt agagaaagaa ctattaagtc gcgtacttta caacaaggaa
      481 ttcaatcaat cgaatctact cgaggtattt caagccatca gcataaccca tttgttactt
      541 taaaaagaaa aaatactgat gaaaatcaag gagaagctat tggtgcagca ctagtttact
      601 caggaaactt cttaattcaa gcagaagtag atacatggga tgttactaga ctacaagttg
      661 gcatcaatcc atttggattt aattggaaac tcaatccagg agaaatattt acagcaccag
      721 aagcgattct ggtttattca agtaagggtt taaatgccat gagtcaaact tttcatcaac
      781 tatttagaaa acgcttagct cgtggtaagt ggcgagaaaa agaccgtcct gtcttaatta
      841 ataattggga agcaacatat tttgattttg acgaagaaaa gttggtaaat attgctaaaa
      901 aagcatttga tgttggtatt gaattatttg tattagatga tggctggttt ggagaaagag
      961 aaaacgattg tgcaggatta ggagattggc atgtaaatcc taaaagacta cctagaggaa
     1021 tcggcagcct ttcagaaaaa attcgtagtt ttggtatgaa atttggttta tggtttgaac
     1081 ctgaaatggt gaataaggat agtgagttat atcgcacaca tccgaattgg attatttcta
     1141 cacctaatcg aaaacctaat catggtagga accaatatgt attaaatttt gggttagatg
     1201 aggttgttga aaatattttc gatcaaatgt gtaaaatcat tgatgaatca aacttagatt
     1261 acattaagtg ggatatgaat cgtccattga ctgatgtatt tgacagtcac tt
//
LOCUS       DASNNU010000216         1294 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.224, whole genome shotgun sequence.
ACCESSION   DASNNU010000216 DASNNU010000000
VERSION     DASNNU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.224"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1244
                     /locus_tag="U8116_002810"
     CDS             <1..1244
                     /locus_tag="U8116_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEN8435730.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DASNNU010000217         1287 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.225, whole genome shotgun sequence.
ACCESSION   DASNNU010000217 DASNNU010000000
VERSION     DASNNU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.225"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..213
                     /locus_tag="U8116_002811"
     CDS             <1..213
                     /locus_tag="U8116_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN8435731.1"
                     /translation="DKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFPFL
                     YPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            210..>1287
                     /locus_tag="U8116_002812"
     CDS             210..>1287
                     /locus_tag="U8116_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN8435732.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQ"
BASE COUNT          483 a          156 c          247 g          401 t
ORIGIN      
        1 gataaatatg atttgaaatc gttggacaga gaattattta tagattctaa attagaagag
       61 gcttatcgaa aattagatgg taatttagaa catcaggatc aggtcacctt atttttagat
      121 tttccatttt tatatcctgg aaaaaatgaa attaaatgga cgaaaaatat tcattcattt
      181 gaaataatgc ctaggtggtg gagaaaagta tgaaaccaag aatatatagt cctactgaaa
      241 cagatttctc aacgaatggc ttaggaattt taaaggattg tacaagatgt ggaatatatg
      301 aagtagctaa tggaaaatat gaattagagt tggattatcc tttaggaact agatttgatg
      361 aatattttga aaatgactat caaataaaag caaagccaaa cgatcaagaa gagtatcata
      421 tcttttttat tgatgataaa gatatagata cttttttaaa tacagtaact atttatgctc
      481 agagtcgtac aaatcgactt ggaagacggg tagtcactct tgtggaagtg gactctaaaa
      541 ctggtcaaga agcgatatca attattgaaa ctaaaatgga taaaaaatct gacatacgac
      601 tttattctga tattacggct gtttctagca caatctttga agcgagaaat gttttaaatt
      661 gtattgctgg tgaacaagga tcattgcttc aatattgggg cggagaaatt aaacgtgaac
      721 catttaagct ttctttgtta aagcgaagag gtcgcaataa tgttggaacg attcggtatg
      781 gaaaagatat gtctggtttg aaggtcaaat tagattggac aggtataaaa acaagaatta
      841 ttccatatgc tgatcctcaa agtgaagtag gtacgactaa tcgaatttat ggttcgccag
      901 tagatagtgc ctatattaat aactatcctg atgtctatac agaacatgtt cagtttacag
      961 aagaacaagg agtaaaagac attaagagct taaataaaat agctaaaaat tactttaaaa
     1021 cgatcaatcc tggctgtgat aagcctaaag tttctattac ggttgaattt gataagttga
     1081 ctgatagtga agaagcgaaa gaatttgcga agattagaaa ttatggtctg tttgatacat
     1141 ttaaaatata tcataaaaaa tataatattt atcttgaatc aaaggttagt ggactacaat
     1201 acgattcttt atctgaaaaa actttgaaat tagaagcagg agacattcaa gttgcttttt
     1261 atcagcaaca agctgtaact attcagg
//
LOCUS       DASNNU010000218         1270 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.226, whole genome shotgun sequence.
ACCESSION   DASNNU010000218 DASNNU010000000
VERSION     DASNNU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.226"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..234
                     /locus_tag="U8116_002813"
     CDS             <1..234
                     /locus_tag="U8116_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN8435733.1"
                     /translation="QTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDI
                     DSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            239..1186
                     /locus_tag="U8116_002814"
     CDS             239..1186
                     /locus_tag="U8116_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEN8435734.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          415 a          183 c          264 g          408 t
ORIGIN      
        1 cagacacctg aagtttttga aatgaatgcc tcaatgtatt cttttaaacc tgaatttcta
       61 aaaaaaagta attatatttt tgatgggcaa tgtgggataa taaaaatgag agattatcta
      121 attttagata ttgatagtga agaagatttt aaatggttag aatttttgtt tcctaaattt
      181 ttagaagatt cttcggagct tcaagatatc tataattatt tatatcggag gtaaaaggat
      241 gagctgttat cttatcaccg gtggggctgg cttcattggt tctaccttag ccaatcattt
      301 aggagaagaa catgtggtaa tcgtcgtcga tgacttatca atgggaaaag tagaaaatct
      361 tgatatgacg aaaaatatca cttttattga gggagatgtc gctgagaaat ccttgatgga
      421 agagatcatg aaagctaata aatttgatta tatctttcat ttagcagcag ttgctagtgt
      481 ggcagattca gttgcccgac cagtagaaac acatcgtgtg aattttgaaa gtgtattaat
      541 gattttagaa ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc
      601 agcggctgtt tatggggatg aaccgacttt accaaaaaaa gaagagtccg tgattcgccc
      661 attgacgcct tatgcggttg ataaattcgc tgcagaacaa tatgtgctag attattgtca
      721 tttatatgat gtacccggta gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa
      781 tccgaattct ccatacagtg gtgtgatttc tattctcgta gatcgctata agaaacaaat
      841 cgtaggagaa gcaacttcat ttacattatt tggtgatgga agccagtcta gagactttgt
      901 ttatatagat gatgtaatcc aagcattact tttagttgcg aatgaggaag ctgcactggg
      961 gaaacaattc aatgttggaa cgggtaaagc aaccacactt ttggaattga ttcatgcaat
     1021 caatgaaatt ctcggtgtag aattgacact tgaatacaaa gaagagcgtt cgggtgatat
     1081 ccatgactct ctagcagata ttactaagat ccagtcaatt ggctatcaac cgaaatatgg
     1141 tattttaagt ggaatgacta attatttgaa aactgaaatc aattaaaaca tactaagata
     1201 agattagagg gtaaattcag gctctttgtc aataaggact gatgagtttt gtaaaatcaa
     1261 atctgggata
//
LOCUS       DASNNU010000219         1268 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.227, whole genome shotgun sequence.
ACCESSION   DASNNU010000219 DASNNU010000000
VERSION     DASNNU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.227"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            66..1244
                     /locus_tag="U8116_002815"
     CDS             66..1244
                     /locus_tag="U8116_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN8435735.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
BASE COUNT          400 a          275 c          267 g          326 t
ORIGIN      
        1 aaaaaggaag tcccttcagt agaatagagt taccacaaca cattcacaga aaagaggact
       61 tccatatgaa cgattttact acagaaattc taaagactct agcgaataaa ggcgatttga
      121 atgaattatt ccgtgtccat ttggaaaaag ctgtcaatac gcttctcaaa acggagttaa
      181 cggctttcct cgattacgaa aagtacgatc gcattggttt taacacgggt aattctcgta
      241 acggctccta tgaccgtacg gtcaagaccg agtacgggga acttcatctc cagattccgc
      301 gcgaccgcaa cggcgagttc aagcaacaga ctgttcctgc ttatagacgg acgaatgata
      361 ccttagagga gaccgtcatt cacctcttcc gaaaaggtat taccatgtcg gaaatcgcag
      421 acttgattga gaaaatgtat gggcatcact acacgcccca aaccatgtcc aatatgacta
      481 aatcatttac ggaagaagtc acttcattta aggaacgtga gcttcatgac cgttatgccg
      541 ctatttatat ggacgcaacc tatatccctt taaagcggaa aaccgtcgcc aaggaagcca
      601 ttcatatcgc agttggcatt cgcccggacg gttcaaagga agtgttgagc tatgcgattg
      661 caccaactga atctatcacg atttgggaag agattttaat agacctccag gaacgtggcg
      721 tgaaaaacgt cctccttttc atcacggatg gcttaaaggg gatggcaggg gccgtgcagc
      781 ggttctaccc caaagcccgt tttcaacatt gctgtgtgca cgtttcccgt aatatcagtc
      841 acaaagtgcg tgtcgatgat cgtaaggaag tctgtgatga ttttaaaatg gtgtatcaag
      901 cgtcatctaa aaaggcggca ttggaagcac gtggtgcttt tgcggaaaaa tggaaaacca
      961 gttatccaaa aatggtggaa tcgatccttt cgaacgatta cctgctcact ttctatgatt
     1021 ttccattagc catacgcaag actatttact ctacgaactt gattgaatcc tttaataagc
     1081 aaatcaagaa atacagccac agaaaagagc agtttcaaaa tgaagaatca ctagagcgtt
     1141 tcctagtatc catctttgat acatacaatc aaaaattctt aaatagaagc cataaaggct
     1201 tccaacaagt gacggataca ttagcttcaa tgtttactga gtaacccatt attttgcaga
     1261 aggacgag
//
LOCUS       DASNNU010000220         1223 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.228, whole genome shotgun sequence.
ACCESSION   DASNNU010000220 DASNNU010000000
VERSION     DASNNU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.228"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..484
                     /locus_tag="U8116_002816"
     CDS             <1..484
                     /locus_tag="U8116_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN8435736.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
     gene            complement(691..1119)
                     /locus_tag="U8116_002817"
                     /pseudo
     CDS             complement(691..1119)
                     /locus_tag="U8116_002817"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1123..>1223)
                     /locus_tag="U8116_002818"
     CDS             complement(1123..>1223)
                     /locus_tag="U8116_002818"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN8435737.1"
                     /translation="LKERGLSGVKMVISDAHIGLTKAVKEYLLCAS"
BASE COUNT          388 a          232 c          236 g          367 t
ORIGIN      
        1 aaaggaagcg atcgatcaaa tcaagacggc tgtcttgtat cctcataacg gattaccgat
       61 tttattgatg ggtgtaagcg gttcaggaaa aacctttttg gcagaaaaaa tttatgaata
      121 cgccaaggaa caacaggtga tagaatcaaa tgctccattt atcgcctata attgtgctca
      181 atactttaat ttctcgatta acaagatcgt agaaaattta acgatccttg atgcaatgaa
      241 ggttttgaga ttggtacaaa aggccgtcaa agaactcgaa aaactaatgg ggatcgaatt
      301 ttcaaacaat caattgttct tgctttacat gcactgctgc tcaatgatcg aacgtatttt
      361 acgcaaagaa agtgtagatg aacaagcaga tatcaaagaa tatattcaaa aagagggaca
      421 taattatgaa atatcaaaat acccggctta caaaacgaag tttgaaagta ctgtaataaa
      481 gtaagattta aataagcata aacaaggtaa gaaccatagg ggctgtgtca agtccaaaat
      541 cttgtgtaaa aaggaaatct caaagtacga tgttttgcct tgttgagcct agctacggtc
      601 caatatgctg gtaagaagtg tcaagagcaa aaagcgaagc gtctcttgat acttctcacc
      661 agtatattat actattggcg gcttaatgtg ctaaatctcg ttacgatcaa attcaagata
      721 ttttctaggt gcagaagtcc attcgtcatc cttttccatc aataaagcac cgatgatacg
      781 aataacagaa tcacaatttg ggaaaattcg gacaacacgt tcacgacggc gtacttcttc
      841 atttagtctt tcaagaacat tcgttgttct caatcgttga cggatatttt ctggtaaaac
      901 cattacttgc atggcatctt cgaagccctc atccaatatt ttacaggcag ttgcgacttt
      961 cttgtcttcc ccatatttat ccataataag ttgtttacgt tctccagcca gctcgatgct
     1021 tgccgttttg aagatggctt taaagtcttc tttgatatcg cttgagattt tcttggagaa
     1081 tttgtcggaa atgttgcgaa ggagatgtgc ttggcatctc tgctagctcg cacaaagaag
     1141 gtattctttc acagcttttg tgagccctat atgcgcatcc gagatgacca tctttactcc
     1201 tgaaagaccg cgttctttca atc
//
LOCUS       DASNNU010000221         1215 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.229, whole genome shotgun sequence.
ACCESSION   DASNNU010000221 DASNNU010000000
VERSION     DASNNU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.229"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            414..947
                     /locus_tag="U8116_002819"
     CDS             414..947
                     /locus_tag="U8116_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEN8435738.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DASNNU010000222         1193 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.230, whole genome shotgun sequence.
ACCESSION   DASNNU010000222 DASNNU010000000
VERSION     DASNNU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.230"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1076
                     /locus_tag="U8116_002820"
     CDS             <1..1076
                     /locus_tag="U8116_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN8435739.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DASNNU010000223         1178 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.231, whole genome shotgun sequence.
ACCESSION   DASNNU010000223 DASNNU010000000
VERSION     DASNNU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.231"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..660
                     /locus_tag="U8116_002821"
     CDS             <1..660
                     /locus_tag="U8116_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEN8435740.1"
                     /translation="GFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCF
                     IDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAK
                     VIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVS
                     RKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDV
                     E"
     gene            657..>1178
                     /locus_tag="U8116_002822"
     CDS             657..>1178
                     /locus_tag="U8116_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEN8435741.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAP"
BASE COUNT          387 a          157 c          259 g          375 t
ORIGIN      
        1 ggatttaaaa ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg
       61 tatgggttgc cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct
      121 gtagattgtt tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct
      181 agttctgaag atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc
      241 gttgggtact ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt
      301 gctaaggtaa ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa
      361 gcttctttag atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata
      421 tttttaggtg atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca
      481 agaaaaagcc tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct
      541 cttacaataa aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc
      601 aaaaaagtta aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga
      661 aaaaaatact tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg
      721 agaatttact acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc
      781 ataaatatgg atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag
      841 ttcaaagttt gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga
      901 taaatggttt cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg
      961 atagaggaga aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac
     1021 atattcacgg aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga
     1081 aaatagcatc tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg
     1141 gggaagattc ttggagagtt tttcccgtcg gagctcct
//
LOCUS       DASNNU010000224         1152 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.232, whole genome shotgun sequence.
ACCESSION   DASNNU010000224 DASNNU010000000
VERSION     DASNNU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.232"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            67..1107
                     /locus_tag="U8116_002823"
     CDS             67..1107
                     /locus_tag="U8116_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN8435742.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          472 a          171 c          196 g          313 t
ORIGIN      
        1 ttgctatttt cgaaaagagt aggtaatttt acataaataa aacaacgtga aagtagagga
       61 tctacaatga ctgattttaa attttttaag gcggatagag tttacaacga attattttat
      121 caatttccaa aagtctttat tgtttctgac gaatacaaaa aaatgaaaga ttcaactaag
      181 attgcctata tgcttttaaa agcaagatta gagatcgcaa tcagcaaacg gcaaatcgat
      241 gaagaaggta atgtttattt tacttatacg acaaatgaac tatgtagagt attaaactgc
      301 caaaaacaaa aagcgatagc aatcaaaaaa gagttggaat cctttggttt attattacaa
      361 aagcagatgg gatttaacaa acagttaggg aaaaataatc ctaatagact atatctagca
      421 gaattaaaag tctcagaaaa tgatatctac ttactcgaaa aatttgatag agagaatagg
      481 gaaaacgttg ataaatcaga gggtatgaaa atcataccca ccctcgacga aaaatcagac
      541 gctgaatccc ttggggctca agagggtatg aaaatcatac cgtgccaaaa cgttgataaa
      601 tcagagggta tgaaaatcat accagaactt aataataata tattagacac taatagacac
      661 aatatagaca ctgaaaaaga ccgcctacaa gatcaattgt tgttagacaa ttttgagaca
      721 attatgacaa acgacagcat tgctacgttt gtccctgaac gagtattaaa tttgataaaa
      781 acattttctt caagttacag tgaagctcaa aaaaccgtcc agactattca taatgcaaag
      841 aaaaaagctg aaatagaaag tgatatttcg atagtttttg aagaactcga tagttattat
      901 gtcaatgcag aacaagaatt atacacgaca ctgttaaaag cctatcaaaa attaaaaacc
      961 gaaaaagtcg aaaatatcca gaacctgatt tttgtctatg taaaaaattg gtttatcgaa
     1021 aaaccaatag ctgctaaagt atcaagtgaa aaacgtttga attatgcaag ctccccaagc
     1081 actattacga aagactggtt agagtgaaac tttactgaca aagtgattgt tgcttcatgg
     1141 agtaacatta aa
//
LOCUS       DASNNU010000225         1109 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.233, whole genome shotgun sequence.
ACCESSION   DASNNU010000225 DASNNU010000000
VERSION     DASNNU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.233"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1030
                     /locus_tag="U8116_002824"
     CDS             <1..1030
                     /locus_tag="U8116_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN8435743.1"
                     /translation="SDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILR
                     SLRPKTVSLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRD
                     YFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQ
                     VMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDD
                     SLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALR
                     VPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQLTKARTT
                     TRLDKIAV"
BASE COUNT          336 a          256 c          219 g          298 t
ORIGIN      
        1 tagtgatcgt cgctgctcca ttgctcgcag agtcaaacaa gccattcttg agctattaag
       61 cgaacctctt tctatgtctt tgatcgcacg tatgaagcac atttctccga ccactgtcat
      121 tcgaatactt cgcagccttc ggcctaaaac cgtttctttg aaccagccgt taccagaagt
      181 ggtgtgtttc gatgaattca aatcggtgaa aaacgtctcg ggagccatga gctttgtcat
      241 gatggacggc aagacccatc aactgattga tattgtggaa aatcggcaac taaatccttt
      301 acgcgactat ttcttgcgct accctagaaa agtccgtgaa caagtacgtc tagtggtttc
      361 agatttctat acaccctatc gcacattggt gaaagagtgc tttccgaatg cccgaatcgt
      421 cgccgatcgc tttcatatta gccaacacat cggtcgggcc tttaccaacc accgaattca
      481 agtaatgaag acctttaaaa aaagggatcg tcggtcgaag cacttaaaga aatactggag
      541 actacttcaa aagaatgcgt gggaattgaa aggtcagcat cgctactggc gtccgtcatt
      601 tcgagatcat ctgacagaag cagagatagt cgatcggcta ttatcttatg atgactcctt
      661 aaagcgaggc tacgaagtct accaggattt tctttccgct atcagaagac aagatgttcc
      721 tgaattcgtc gcgctcctaa aagaagacta caaggagttg ccagagcact accaaccagt
      781 gttcacgacc ttcaagaaat atcgaacaga aatcaaacga gcgttgcgag ttccctattc
      841 caacggccct atagaatgtt tgaacaacca tatcaaagta ctaaaacgaa ttgcctatgg
      901 ctttagaaac tttcaaaact accaagaacg aatcttttta tatcgaggaa aatattttaa
      961 gaaaactaag aacactaccc aactgactaa agctagaaca acaacacggc tagacaagat
     1021 agctgtctag ccgtgtgagt atctcgatta ttctattgaa tttcggctct atactttttg
     1081 atgttggtga acttcaccaa catcttttt
//
LOCUS       DASNNU010000226         1073 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.234, whole genome shotgun sequence.
ACCESSION   DASNNU010000226 DASNNU010000000
VERSION     DASNNU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.234"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1073
                     /locus_tag="U8116_002825"
     rRNA            <1..>1073
                     /locus_tag="U8116_002825"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DASNNU010000227         1062 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.235, whole genome shotgun sequence.
ACCESSION   DASNNU010000227 DASNNU010000000
VERSION     DASNNU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.235"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..993)
                     /locus_tag="U8116_002826"
     CDS             complement(37..993)
                     /locus_tag="U8116_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435744.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DASNNU010000228         1056 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.236, whole genome shotgun sequence.
ACCESSION   DASNNU010000228 DASNNU010000000
VERSION     DASNNU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.236"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            60..554
                     /locus_tag="U8116_002827"
     CDS             60..554
                     /locus_tag="U8116_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435745.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            568..999
                     /locus_tag="U8116_002828"
     CDS             568..999
                     /locus_tag="U8116_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435746.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          396 a          151 c          228 g          281 t
ORIGIN      
        1 gacaaagcta aactcgatgg attagccaat attacatttg agaaagtggg gacggtttaa
       61 tggctgatat tgtgcaatta aaagaagacg gagttgctaa atacttaaaa acgcatgtag
      121 aagctattga tggtaaagaa gcattggtgc agacagatgg agaccaagcc attgcaggac
      181 ataagaattt ttcaggttct gtaacaatca ataataaacg tgttttgacg actgatgata
      241 acagatatga agtggtaaat ctggttgtca ctaacggtaa tacagggaca gcaaagcttt
      301 atcgtgaagg aaaaacagtt tctatatatt ttgtggcttt aaacggaaaa agtagtggcg
      361 ggaatgattc ggttatttta actgttccag aaggctatcg gacaccaatt agttttgaac
      421 aactggttgg gtcaatagac cgttctactt tgaacagtgc tcaactatct attggcgcag
      481 acggagccat taaatggaga agaaattcaa gttatggatc agcttattca tttgttatca
      541 cttattcaat ttaagggagg aaatctaatg aaagtagttt acaaatcaat caagccttac
      601 ggattcgagc aaatcatttt gaacaatcaa gaaaatatcc ctgaaaactg tacagagatt
      661 aaaccaccag ttcctaactg gagaccaaga tttgattttg ataaaaaaca gtgggttgag
      721 ttagctactg aagaagagaa aaaagggaat gctgttgata gcgtagatga attggctaat
      781 attaaggcat tatacgaaac gctgaaggca gaaaatgatg aattgaaaca gctaaattct
      841 aaagcaatgc taaacaatgt agcaatcaag caagaaaatg tcttattgaa agaaaagtca
      901 gaaagtttag cgcagttgaa ttcaaaaacg atgcttgctt ctgtacaaaa taccaaggaa
      961 atcgaagaaa tcaaaaaaca attacaaggt gggaagtaac atgtattcat atgaagatat
     1021 caaactaatg tatgactggg gctgtttcac taacga
//
LOCUS       DASNNU010000229         1050 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.237, whole genome shotgun sequence.
ACCESSION   DASNNU010000229 DASNNU010000000
VERSION     DASNNU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.237"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..881
                     /locus_tag="U8116_002829"
     CDS             <1..881
                     /locus_tag="U8116_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN8435747.1"
                     /translation="ISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVIIHLHGS
                     EFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENSIVVPE
                     YTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDEEISLK
                     ELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYGMPVIA
                     TDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAITKFNDG
                     NFFDRILNLYQKGSKE"
BASE COUNT          387 a          114 c          223 g          326 t
ORIGIN      
        1 gtatctcttt attgagtaaa cctaatatta ttcatatgca catgtcgtac agagggagtt
       61 ttagtaggaa atatgtgctg aataagtggt ttagattatt aaatattcct gtaatcatcc
      121 atcttcatgg atcggaattt aaaaaatggt atgatagtgt ttctgtaaaa aaacaaaatc
      181 agattcgctt gctgttaaga gagtctgatt ccacgattgt acttggagaa aaatggaaga
      241 agataattaa agaaattgag ccaaattgta aaactgttgt tgtagaaaat tctattgtag
      301 ttccagaata taccataaag tggaattcaa aaatgtgccg atttctgttt atgggagttt
      361 tgataaagcg aaaaggtgta cacgatttgg tagaagcagt taaaaacatt gcagaacaat
      421 atccagaaaa aaagtttatt tttgagattg ctggagcagg tgatgaagaa atatcgctca
      481 aagaattgat agaagaatat aatttgcaat cgtattttga atttacaggt tgggttgtgg
      541 gagaaaaaaa ggaggagtgc tatagaagat caaatgtgat gattttaccg tcctataacg
      601 agggattgcc tatatctatt ctagaggcga tttcttatgg aatgcctgta attgctacag
      661 atgtaggaga tatttcttca gcagtacgtg atggagaaaa tggatatttg tttcaacctg
      721 gtgacgtatc agcgttgaca tctgcaatac taaaaatcaa tgataaagat aaatacgtaa
      781 aattttcacg ttgttcgaag gatttggcaa taacgaaatt taacgatgga aatttctttg
      841 atagaatttt aaatctgtat caaaaaggga gcaaggagtg atgacaagga aaataaaaaa
      901 tgtttcgatt atgtaatgaa cttattaagt gaataaaata gagagaaaaa atattgaaat
      961 agacgatgta aatttttatc cgtgtaagaa ttcaaatgaa tagtagtgtt ttagtgcttg
     1021 taatagagca agagtacaag cacggtacat
//
LOCUS       DASNNU010000230         1016 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.238, whole genome shotgun sequence.
ACCESSION   DASNNU010000230 DASNNU010000000
VERSION     DASNNU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.238"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..831
                     /locus_tag="U8116_002830"
     CDS             <1..831
                     /locus_tag="U8116_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN8435748.1"
                     /translation="SIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNIVMN
                     VLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNKRK
                     ELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKAKA
                     VELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTIS
                     KDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSRDH
                     "
     gene            864..>1016
                     /locus_tag="U8116_002831"
     CDS             864..>1016
                     /locus_tag="U8116_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN8435749.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDN"
BASE COUNT          394 a          116 c          190 g          316 t
ORIGIN      
        1 tctatagttc acaatcaggt ttattttggc gggggaatta atttatggtt agccaaaaag
       61 aatggaatca gacagagaat tgctcatagc catgcaactg aagatggaaa atctcaaaat
      121 attgtaatga atgtattgcg taaattttta accaaattgt tgttacaaaa tgcaacagac
      181 taccttgccg ttagtcaaga agctggtgaa agtctgtttc aaaaccaccc ctttgagata
      241 gttcataatg gtattgattt agagctttat agtaaaaatt cagaagctaa ggttaataag
      301 agaaaagaat tggatatctc aatgagtact tttgttgtgg gaaacattgg acgattagag
      361 aaacaaaaaa atcaatctta tttgttagag atttttttag aaatactaaa aatgaattct
      421 gattcttact tgattttagt aggtgaaggg tcacttagaa ataaactgaa agccaaagca
      481 gtagaattgg atatagagaa aagagtaaaa tttttaggag aaagaaacga tattcccgag
      541 ttattatctg tgatggatgt ttttgtaatg ccttctcttt atgaaggtct gccaatggtt
      601 ggattagaag cacaagcagc aaaattaaaa ttagtcttag cagatactat ttccaaagat
      661 acaaagttaa caaataatgt ggattttgta tcattagatg aaacaccaca agaatgggca
      721 aaaaaagtaa taaaagagcc gtatgctaat aaaataacac aagaactaaa gagttatgat
      781 gtttcgtaca ctactgaaca aatggagagt atatatagta gagatcatta aaaatgttca
      841 agatatttta ggagtgatta tttttgaaaa tatgttattt gatattggca tacaatgata
      901 ttggcaactt aaagagattg gtcaatcgat tgaacgaaaa tgcagttttt attattcata
      961 tagataaaaa aactgatata gaaccattta ttgaagcatt taaatcatat gataat
//
LOCUS       DASNNU010000231         1004 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.239, whole genome shotgun sequence.
ACCESSION   DASNNU010000231 DASNNU010000000
VERSION     DASNNU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.239"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..653)
                     /locus_tag="U8116_002832"
     CDS             complement(<1..653)
                     /locus_tag="U8116_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN8435750.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIK"
BASE COUNT          331 a          193 c          106 g          374 t
ORIGIN      
        1 tctttaataa aacttgttat ttttgaatgt tgcaaatatg aaatatggaa tatgatatct
       61 tcagatatat aatccctttc tgacggaaac ttgatattgt gctgttgaat tatctcagat
      121 gaatacagcg catgccaaac tgacattggc aatataaaat cgactttata actcggctca
      181 gtgccaatca ttccaagcaa cactttttca attatagcgt tatttgtaaa tatcgtacta
      241 tcatatcttg ctggctttga aataatatta ttatcatcat aatattcata atatccagca
      301 taacatgtgt ctgcattact cctttttata ccatttaaca ttgcttcaat ataattagct
      361 gcaacaaaat catcagaatc aataaaagca acatattttc cttttacatg ttcaagtcct
      421 gtatttcttg ccaatcccaa gccagcatta tttttatgaa taatacgaat ccttgaatcc
      481 tttaatgcat agtcttcaca tattttatta gatgaatcct tagatccatc atccacaatt
      541 ataatatcta ggttcttata tgtttgatta cacactgatt ccaaacaact tcctacatac
      601 ttttcaacat tatatacagg tatgattaca ctaattagat cactcgaatt catccttcta
      661 tcctttcact cacgttttct caattagtca atgacggcat caacgatttt cttaaattct
      721 ctgaaaaaaa tagactctga tcttgatgat gtgaatgaca tactatcttc ttctagaaaa
      781 attatttcct ttaaattatt tattaaatca ataatatgat cctcgtaagt atgctttccc
      841 ttggaataat gaaaatatac ttttgtatta cacaatattg caattttatt tcttaatatg
      901 attatttcac tcattcatta ctgccacata tacacaactt ataaccaccc cctgcaaagt
      961 ttagggtaag ttatcagata atatcttcct cttcatcatc caga
//
LOCUS       DASNNU010000232          993 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.240, whole genome shotgun sequence.
ACCESSION   DASNNU010000232 DASNNU010000000
VERSION     DASNNU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.240"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            360..993
                     /locus_tag="U8116_002833"
                     /pseudo
     CDS             360..993
                     /locus_tag="U8116_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          339 a          181 c          182 g          291 t
ORIGIN      
        1 tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga acaccttcaa
       61 ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct taaacgaatt
      121 actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta gtgcaaatat
      181 taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg atctatatat
      241 tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat ataggctcta
      301 taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat tttaggtata
      361 tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa aggaccctgc
      421 caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat aaaatgagct
      481 ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca cgaaaattgt
      541 ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa tatttagtga
      601 cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat gcgaaagtcg
      661 tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat tttcgtgtac
      721 aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa gtgaaaaaga
      781 actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac aaaacatggc
      841 gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt gatcgattgc
      901 tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg accgaagcct
      961 ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DASNNU010000233          962 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.241, whole genome shotgun sequence.
ACCESSION   DASNNU010000233 DASNNU010000000
VERSION     DASNNU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.241"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(13..86)
                     /locus_tag="U8116_002834"
     tRNA            complement(13..86)
                     /locus_tag="U8116_002834"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Ile,seq:cat)
     gene            complement(641..955)
                     /locus_tag="U8116_002835"
     CDS             complement(641..955)
                     /locus_tag="U8116_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435751.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          354 a          149 c          152 g          307 t
ORIGIN      
        1 tggctacatt aatgtaccct gtaggactcg aacctacgac cggacggtta tgagccgtct
       61 gctctgacca actgagctaa gggtagtggt tgttgccaca taaagccata aacaatcaac
      121 cagtagaatg cgtggcaaca aacctgttat cgcatatctt ggagtgtgac tatttatgag
      181 tgatagtgaa gatatgcgtc tccatatatg tatggttgag acagataaac atgtatgaaa
      241 aacgttgtta aatcaacgtt tttttatttt ggtcaaggag agaaaagtag agaaatacaa
      301 aattttttgc attgattttg cattcaaaaa aacattcaaa catttagaaa tccccaatac
      361 gtattcagga aaatctattt taaggtttac aaaaagaact tttgttcgta cactcttttt
      421 gaggtgaact tttatgctga tggaaggaaa aacacaatta tggtttaagc atgttccttc
      481 aaataagttt tttaattata aactctagag aaattaattc atcctaacat aaaataattg
      541 ttcactcaaa agtttttcta gaacaattaa aacagcaggc ttcacattcg ctatacattt
      601 gtaagaagta ataattcctg tactaaaagg cataattgct ttaattattt tttgacttct
      661 cttcaaacac aagaagtagt tgtgtaataa ttgcagaaat aaggattcca ataatagata
      721 taaaatttga catggaaacg tctataacat taaaagaata aagtgttgaa gaaagtaaac
      781 tgattgaaat aacccctccg taaaaaatag tctgtgaata tacccacttt ttttcagaag
      841 acgtacttcg tttggttcta aaacctatcc ctgcatttct actcttatca aaaaaccaaa
      901 tatttagaag taataatatt gctattatta agataaaatt actgtaaaaa tccaaaagct
      961 at
//
LOCUS       DASNNU010000234          961 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.242, whole genome shotgun sequence.
ACCESSION   DASNNU010000234 DASNNU010000000
VERSION     DASNNU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.242"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>961
                     /gene="bsh"
                     /locus_tag="U8116_002836"
     CDS             <1..>961
                     /gene="bsh"
                     /locus_tag="U8116_002836"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN8435752.1"
                     /translation="SITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVNDLDT
                     HYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWI
                     LGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDN
                     PVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSV
                     SRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNV
                     DKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKY"
BASE COUNT          338 a          127 c          170 g          326 t
ORIGIN      
        1 cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat tatgaaatat
       61 cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt cgaaaggtaa
      121 atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct gactaccctc
      181 tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat ttttctgggt
      241 atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt gaatttattc
      301 cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta aaaaatatca
      361 atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat tggctattag
      421 ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat atatatgata
      481 accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt aatttaaaca
      541 attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa ataagtttga
      601 atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc tcagtatctc
      661 gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca gagtcagaaa
      721 gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt ttgtgtgatg
      781 ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt gacaaaggaa
      841 tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg aataaagaag
      901 acttagatag tcataagtta attagttatc caattataga aaaacaacaa attaaatata
      961 t
//
LOCUS       DASNNU010000235          948 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.243, whole genome shotgun sequence.
ACCESSION   DASNNU010000235 DASNNU010000000
VERSION     DASNNU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.243"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            68..493
                     /locus_tag="U8116_002837"
     CDS             68..493
                     /locus_tag="U8116_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435753.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT          348 a          160 c          162 g          278 t
ORIGIN      
        1 tccatccgtt tgaagtttta cattccaatg gtataatttc tttatcctat caaatggggg
       61 aagtattttg aagaaattaa tcgtactatt tattttgtct gcgggacttt tgacattgac
      121 tggctgtgat ttgtttggcg gctctgacca atcctcggac gaaacaaccg attcttctgt
      181 cttaaaagcg gaaaaagcag ataccaccaa cagtgtatat acgctaaaag tacgtaaaga
      241 tatggattta tccgcgggac agttgacatt tacagataaa aaaatggaat ggacacgcac
      301 gtatcagaca caaggaactg attcttctga atcatctgaa gaaaaaacag tgctgacaga
      361 tatcaagatc actaccaaag atgacgaata tattatttca ggaaaagaaa ataaaaaaga
      421 aacaacagtc atatttacaa aaatcggtaa ttaccgtttg aaggatgagg atggcaacat
      481 ctacagtcta tagtgccgtc tgttggatct tcttttgttt ctccccttct tttctgctat
      541 agtaaagaag caagatgatt gtaaaggaga cgaaatggat gatcgatagt cctgattatt
      601 gtacatgtaa taacaacaaa aatatccata cagaatttaa tgaccaagga agcttgagca
      661 tatgcgacaa ctgtcacaaa ctagtcaaag attcatttaa aacatttgaa tcttatcctg
      721 ttcaaaatta tgtaaaaagt agaagaagat aaacaaatcc aagcaaaact cactatggct
      781 gatttttatg aacttcaaac tcagcttctt cctttgatca agcttcatca gtaaaacaaa
      841 cagtggatca tttcagttta caaatcaaag agtaaagatt tatttatttc aaatatttga
      901 atataaaata accaaacgat agaaaggctt attctacatc acgtagta
//
LOCUS       DASNNU010000236          939 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.245, whole genome shotgun sequence.
ACCESSION   DASNNU010000236 DASNNU010000000
VERSION     DASNNU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.245"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            564..>939
                     /locus_tag="U8116_002838"
     CDS             564..>939
                     /locus_tag="U8116_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN8435754.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQ"
BASE COUNT          334 a          147 c          166 g          292 t
ORIGIN      
        1 ctgtaataaa tggtgtatct gttcttggat aactcaataa ttttgcttca tacagtcctt
       61 gcatagcttt taaacgtaaa attttgcgtt ctaagacgtt ttaaatatat ctgcgtatat
      121 ttttatgtgt tttatctttt cgtcggcaaa tgcgttaaaa tttgcgaaat aaaatagctc
      181 aagggcgaac gcagtgagtg catttaccct tgatctattt ttttgagtaa attagctttt
      241 gaaaccgacg gaaaggaaac acaaaacttt tcagaggcaa ggggttttta aggggcgctt
      301 ttagcccctt gaatttattt gctttaagat atatttagtt ttaaaatagt tttaacgaaa
      361 aaaagacata aaaaaagacg agcaacgcta ccaacgttct cgcccggaat aacaaaccac
      421 acttgttatt cattcgatga ttagtatatc aatttttgat tttgaataca aaacaaaatg
      481 gacgaatgga tacaggcgaa atttattttg cgattccact cgtccttttt tcgttccaaa
      541 aattatttta ggagcgataa aaaatgaaga aaattgatga aaaattagct gcagaaatct
      601 cactaaaaaa accggccgtc tctaatagcc ggttaagtaa tagccggtta agtggtcaaa
      661 ctttgggaaa atctcaaccc gcattaagtt ttgatgccat gacaatcgtt ggaaatttga
      721 acaaaactaa tgctaaaaag ctatctgact ttatgagtgt agagccacaa atacgacttt
      781 gggatatact tcaaacaaag tttaaagcta aggcacttca agaaaaagtt tatatcgaat
      841 atgacaaagt aaaagcagat acttgggata gacgtaatat gcgtgttgaa tttaatccca
      901 ataaactcac acatgaagaa atgatttggt taaaacaaa
//
LOCUS       DASNNU010000237          921 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.246, whole genome shotgun sequence.
ACCESSION   DASNNU010000237 DASNNU010000000
VERSION     DASNNU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.246"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            522..908
                     /locus_tag="U8116_002839"
     CDS             522..908
                     /locus_tag="U8116_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435755.1"
                     /translation="MKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRFEVLKIQKNE
                     LLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYV
                     EDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          339 a          113 c          166 g          303 t
ORIGIN      
        1 tatggttcgc tggtaaaaaa tatattacta ctttcaaaag attatccgag ttaatgcagc
       61 agactgttaa aagtgctatg cctgaaggat acaagtttaa ttttgttcaa tcttcagtat
      121 ctactgaaag tatctatttt tcaattcttt ctgagagtag gctttttcat tttagaataa
      181 gttctcatgt aaagaaaact gatgttgata tatatacgtt ccgtacaaat aattataaaa
      241 atttaaatga tttgagtaga agtatcagtg aatatctcaa ggggaatagc tatctcaaga
      301 ttcgattttc acatatatta attttactac catatttgaa tgagaatttc accctaattc
      361 aagtagataa gtctttcatt gcctcaggta atcaaggaaa tatgaatatt acaagtaaca
      421 aaacgtcacg tgtgctattg gcgttgatgg gttacggaat tctttataga gatgaccaaa
      481 atggccagat tctaataaat cgggctgggc gttcattatt aatgaaatat agaaaattta
      541 taccggaaga ttttcaaaac ttacagttag aaaacatgcg tttagaagat ttatttagat
      601 tagcagaaga tgataagtat agatttgaag ttttgaaaat tcaaaagaat gaactattga
      661 ataagttata tcctgagaat gcgataaaag cacattttgg tgatgctaat catatgtata
      721 gttggttcat tcctaaaaga tttagaggga aaatcaatgc tggagatcaa gtgaaagtac
      781 tcaatcgaaa tagtacagat gtcgtaatga taacagatat ctatgtagaa gatgaagata
      841 taatcaaata tatgaagcct gtgataaaac ttgttaataa aaataaagga acccgtggtg
      901 ctagttaatt ttttcctaga g
//
LOCUS       DASNNU010000238          919 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.247, whole genome shotgun sequence.
ACCESSION   DASNNU010000238 DASNNU010000000
VERSION     DASNNU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.247"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            83..316
                     /locus_tag="U8116_002840"
     CDS             83..316
                     /locus_tag="U8116_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEN8435756.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            313..720
                     /locus_tag="U8116_002841"
     CDS             313..720
                     /locus_tag="U8116_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN8435757.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          342 a          128 c          159 g          290 t
ORIGIN      
        1 ttagacatga gagggactat gcttgattta aaatcaacaa gcgtatatac tgtatataca
       61 aagaaaaagg agggagaaga ctatggcaat taaagaaaga aaatttaggg aagtggggaa
      121 ctcagtagta ttaactattt caaaggagtt tcttaaagaa aatgggttaa atcccggaga
      181 tacggttttt ttagatgaag aaaaacttcg agcggcaatt acaaaaaaac aagccttatc
      241 tgatgaaatt gatttagcaa tatctcaagc attaactgaa tatgatacta cttttagaga
      301 gcttgtcgat aaatgagtat tcattaccta gaagccaaag atattataaa aatgaatgta
      361 atccaaataa aaaaatactc tcctaatgaa ccccttggtg taaaggatgc gaatgcttta
      421 gaaatgtgtg tagggcaact tcgagcctca gcttttggtg aagaggttta tccttcgatt
      481 tatgaaaaag cagctatcct acttatacaa ttgataaaga aacatccttt tcacaatgca
      541 aataaacgta ctgctttctt agctactttt gtctttttga aaatcaatgg acagctttta
      601 gctattgatc aaaaagatgc tgtaaattta gcggtatata ttgctacgta tgataaggat
      661 ttcgatcaac taaaatatga agtgatcaac actataaaga cacatacgca gcccatttaa
      721 ggagttactg tatgtgtctt tatagggtta ttagctgcta tagcgaaata attgaagtaa
      781 attacaaata gctataattc aaatccaact atatagaaaa cgtaaatttt ctatatagtt
      841 ggattaagat aacgactatt tattttatca tatttttact ataccctata cttttacttg
      901 gtatatttat gaataatcg
//
LOCUS       DASNNU010000239          915 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.248, whole genome shotgun sequence.
ACCESSION   DASNNU010000239 DASNNU010000000
VERSION     DASNNU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.248"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..911)
                     /locus_tag="U8116_002842"
     CDS             complement(<1..911)
                     /locus_tag="U8116_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN8435758.1"
                     /translation="MTITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVANNQY
                     ILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANA
                     NSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQ
                     NIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLT
                     DGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDV
                     NQAYNTSGNVGYHVSFNMRNFPNKKVYV"
BASE COUNT          258 a          186 c          165 g          306 t
ORIGIN      
        1 acatagactt tcttattagg gaagttacgc atgttaaaag atacatgata gccaacattt
       61 ccagaagtat tataagcttg atttacatct gatctataaa ttccatcagc tcttactcga
      121 gctaattctt tcccagtatt gtaatccata atgaagatat actcgtattt atagttagca
      181 atgtgccatc cagctacatg caaatttgca ttttcgattt ctccaaactg atcaatgtgg
      241 gcgtaattcg ttccatctgt cagtgtagga ttagctgcac cagctcgtgt tggatcaatg
      301 acaggcttgt tttcagaagt tgttggattt tcatcggtaa atccatgagc taaatcatat
      361 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
      421 ccccagatgt tttgtgttac ccataaatgc gatttgattc ccggttggtt ataaggagtg
      481 tccaacgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      541 ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt gtaactcaat ctgcacagga
      601 ctgttggcat tagcatacga accagcaccg tactgtacat aaccaggttg accgacttga
      661 taaacaattc cgccgtctcc cacaatataa gcagtataag cgctagtcca tgaacgttgc
      721 atatactgcg cttcattgcg tcctgttgct gtttcattag ccgtttcatg cagtaaaata
      781 tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
      841 atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag gctcatcgca
      901 gtaatagtaa ttttc
//
LOCUS       DASNNU010000240          902 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.249, whole genome shotgun sequence.
ACCESSION   DASNNU010000240 DASNNU010000000
VERSION     DASNNU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.249"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..282)
                     /locus_tag="U8116_002843"
     CDS             complement(<1..282)
                     /locus_tag="U8116_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN8435759.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTK"
     gene            complement(384..737)
                     /gene="tnpB"
                     /locus_tag="U8116_002844"
     CDS             complement(384..737)
                     /gene="tnpB"
                     /locus_tag="U8116_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEN8435760.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(727..>902)
                     /locus_tag="U8116_002845"
     CDS             complement(727..>902)
                     /locus_tag="U8116_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435761.1"
                     /translation="VPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNYI
                     LQAILKELLSNAL"
BASE COUNT          258 a          185 c          158 g          301 t
ORIGIN      
        1 tttcgttcct acaactcgtt gatgtccttt tactggaata atggtggcag ctgcttctgg
       61 aacacttaca gaaatttctt catcactaaa aagattcaat tgcccattcg tttcctcaaa
      121 gagagtctct ttagagcgtc caaataattt gttggttaaa taattgacct tttcattcaa
      181 tagttggatt tcagttgtaa gattctgaat ggtttgttgt tgtgcttgat tggttttatg
      241 ggcttcttga agcaactgaa gcaacgtatc tgtagtttcc aaaatttttc accaccttga
      301 atcgaactga ttttgttaca gaataccaca agaaacgcac aagaaatagg ggttcaacac
      361 attttttgtg ttaacccccc agcttaaaaa actccttttt ttcctggcaa aatagctcga
      421 ggttgatcga tacttaatcc ttctaacaac catcgaacct gttggttcgt taaggttcga
      481 acttcgtctt tcgtccgagg ccattttaac ttgccgtttt ctatccgctt ataacagaga
      541 agaaaaccat caccgtccca atagagaagt ttgaaacggt cttgacggtt cccgcaaaat
      601 aaaaaaacgg catcttcgta aagctcgagt tgatactctt gttgaataag actggcaaga
      661 ccatcaattc cttttcgcaa atctgttttt ccgcacacaa tataaatatg tttggtttga
      721 agtaaatcat aaagcattgg acaacagttc tttcaaaata gcttgaagaa tataattgtt
      781 aataccgtta tagatcaaaa cttcagaacc attctctctt ttgatgcgca tcgctaactt
      841 ggcagccgcg ggtttacctt tttctggtgc actcttttcg tttcctaatt ggacaggaac
      901 aa
//
LOCUS       DASNNU010000241          873 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.250, whole genome shotgun sequence.
ACCESSION   DASNNU010000241 DASNNU010000000
VERSION     DASNNU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.250"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(323..544)
                     /locus_tag="U8116_002846"
     CDS             complement(323..544)
                     /locus_tag="U8116_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HEN8435762.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          283 a          185 c           80 g          325 t
ORIGIN      
        1 cctaagctct ataatatctt cttaaaaaat aatattatgg tgtcgttttc aaactcgtaa
       61 tcttttacat tggaaaaata catacttttc tcaatgtagc aactcctatc tcacagtatt
      121 caaaaattct tcaatattat gaaatacttg tgcatgttct ttaaatacct tgtcagtctg
      181 tttataatcc cctactaaat tttgcattgc tcccaactct tcgacaaatt gtcatctcca
      241 tctgtaaaca gccctaaaaa ctcatataat gctttttcat caatattttt cccatattat
      301 tctcggtata gtttcacaag ttttacggat tcttcatatt gtttatcttt ttataatatt
      361 catctaggga cataattact tttgctagat ttcctgcaac aacacaatcg tctgatacgt
      421 ccttgtttac taaactattt gcacctataa ctacattatt tcctatatgt acacccttta
      481 gtattctaga cttcattcct ataaaaacat tattacctat tttgatcctc tccgatgatc
      541 ccaaagcttc tccatactac ttttaaaacc gatcaatcat aaacatgagt caaaattgta
      601 actccttcat atcatctcca atttcaacaa gagtcttcgt tggaacaaaa actatacatc
      661 gttcgcctat tcggcagcct aaaaattgta gttactttat atatcattct gatgtctctt
      721 ctcatctttt taccaatacc gacgtaagaa ctttcaaaat atacttaaac ttcttattac
      781 ggatattaaa cagaagaata actaaaatcg atatgacaac cgatataact aaatttatca
      841 ctaaacttat cactttgaaa tttccgatat taa
//
LOCUS       DASNNU010000242          863 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.251, whole genome shotgun sequence.
ACCESSION   DASNNU010000242 DASNNU010000000
VERSION     DASNNU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.251"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..792
                     /locus_tag="U8116_002847"
     CDS             <1..792
                     /locus_tag="U8116_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN8435763.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
BASE COUNT          317 a          117 c          145 g          284 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aat
//
LOCUS       DASNNU010000243          839 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.252, whole genome shotgun sequence.
ACCESSION   DASNNU010000243 DASNNU010000000
VERSION     DASNNU010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.252"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            107..>839
                     /locus_tag="U8116_002848"
     CDS             107..>839
                     /locus_tag="U8116_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN8435764.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDK
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQR"
BASE COUNT          306 a          152 c          141 g          240 t
ORIGIN      
        1 tggtgctagt taattttttc ctagaggaaa atgtctaaac atgttggtaa atcaacaaaa
       61 aaacgcatat agtagtctca aaactcccaa gaaatgagga ctacatatgc gttcatcaaa
      121 atatactgaa cattatacag atacttttat aacattcaag gaaattatga gaactgtttc
      181 taacatatac cacaattgtg taccagataa gattaaaaac cgaagaaata ctgataaact
      241 gaagcaacgc gatacagtga ttattgcttg tgttatatgg ggcataatta atggctatac
      301 tagccaaaga gccacgtata gagcggtttg ttccgtctta tttcctaatg gtgactttcc
      361 tagtaggagt cgattcactc gcttaagttc aaacttagct tacactatca agattattcg
      421 atactttttc atcaagaagc tcaccaaagg tgaactagtc ggaattatag atagttttcc
      481 tagtccatta tgtaaaccag ttagaaatag acaagcaaaa ctattgaatc aaatagccaa
      541 agttggctat aactcaacaa aaaaatctta tttttatggt cttaaaattc atatgatcgt
      601 tactaagact ggctttccga ttacttactc aatcacgaat ccaggtgtcc atgacgtgaa
      661 agtgttagag actttatcgg aagaagcgaa tcttcccaat atcctagggg acaaaggata
      721 tattagccat aaaatccatg aaaagcttgc ccttaagggc attactatat ccgttccgcc
      781 acgtaaaaac atggataaat cagaaaaact agaccacagc ttacttggaa aacaaagaa
//
LOCUS       DASNNU010000244          836 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.253, whole genome shotgun sequence.
ACCESSION   DASNNU010000244 DASNNU010000000
VERSION     DASNNU010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.253"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..244
                     /locus_tag="U8116_002849"
     CDS             <1..244
                     /locus_tag="U8116_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN8435765.1"
                     /translation="SIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVFFFVYNTTL
                     RGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            241..>836
                     /locus_tag="U8116_002850"
     CDS             241..>836
                     /locus_tag="U8116_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN8435766.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTI"
BASE COUNT          293 a           96 c          171 g          276 t
ORIGIN      
        1 ttcgatattc ctatttttta taataaagac atttttcaat gcaaaaagat cttttatgaa
       61 ggataaagat gcaagtcttg ttaaagttat tgcaattatg tcggtattct tttttgtgta
      121 taacacaaca cttaggggtg aaacaacggg gccactgatt tatatgattt tagccatgcc
      181 tgaagttgtg aaaaggaaag agcattatta tagtgaaaaa aatacaaaag gagagatatt
      241 atgattccta agaagataca ttactgttgg ttcgggggaa atccacttcc gaaaaaagat
      301 agaaagtgca tagaaagctg gaaaaagtat tgccctgatt atgagattat tgaatggaat
      361 gaatcaaatt atgatttaaa taagaaccgt tatatggccg atgcatataa agaacgtaaa
      421 tggggttttg ttccagatta tgcaagattt gatattattt ataatcatgg tggattttat
      481 ttagatacag atgttgagct aattaagtcg ttagatatac ttcgtgaaaa tcatgcctat
      541 atgggatttg aaggggaaat ctgggttaat ggcggtattg gttttggtgc agaagaagga
      601 cattctgtta ttaaaggttt aagggatatt tacgattcat taagttttta tcaaaatgat
      661 ggtacattga acttgaaacc gagtccgtat tatattacag attatcttga atctattgga
      721 ttggttcgcg ataacagcga acaaataata aatggcctgc atatctatcc tacggattat
      781 tttgctgcca aggattatga tactggaaaa atttataaaa ctagtaatac aataag
//
LOCUS       DASNNU010000245          823 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.254, whole genome shotgun sequence.
ACCESSION   DASNNU010000245 DASNNU010000000
VERSION     DASNNU010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.254"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..534)
                     /locus_tag="U8116_002851"
     CDS             complement(<1..534)
                     /locus_tag="U8116_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN8435767.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMF"
     gene            complement(610..756)
                     /locus_tag="U8116_002852"
     CDS             complement(610..756)
                     /locus_tag="U8116_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEN8435768.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT          328 a          133 c           89 g          273 t
ORIGIN      
        1 aaacatttga atgattaaaa atattaagac accaaacaca aaatgatcaa atacttgata
       61 ttgatgagca acataagaaa caaataaacc aattgtaagt aaataaatca tgaatatagc
      121 agtaagttta gaactggact gttcaacaat cctatttata gctaattttc tatataacca
      181 aataaacatc agcaaaaaca aagaatcaag cattatctgg aatactacat aaccataccc
      241 ttttatccct tcaaatgaaa acatatacgt cataaccatg gaagtaataa tagatctgaa
      301 atcatcaata agcatacata atagtaattt atttaaaagc actatattag ttttcttctt
      361 ttcctttaat ataaaataac ttccgattat aggtatagct gcggaaacat cagtaaataa
      421 ttcaacaaaa aaagtgagtg gtatcaataa acatacaaaa agatattttc ttttgttaag
      481 atcagtatta gtcatataaa ataagagaaa aaagaaaaag tttcccacga acattcttaa
      541 tagataagct tttgtatcga tagtaatcaa gagatcccct cctgatttta ggatatcata
      601 aaaaaaattt tagttacact ttttaaataa tgaaaaaaca cattccacta aatttgatgc
      661 cggttttcct tgaggttttg ttccagcacc tcctttaatt tttttaagct cttgatctga
      721 acattgttta gcatttagtc tatttttttc ttccattacg attcccccta caaactataa
      781 atacttttat gtcactgatt caatatacct atttttctaa tca
//
LOCUS       DASNNU010000246          823 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.255, whole genome shotgun sequence.
ACCESSION   DASNNU010000246 DASNNU010000000
VERSION     DASNNU010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.255"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..>823
                     /locus_tag="U8116_002853"
     CDS             22..>823
                     /locus_tag="U8116_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN8435769.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT          253 a          142 c          186 g          242 t
ORIGIN      
        1 cgaggagggt ggaaagaaac cttgccagaa attgcggatg aaatcagtat cctcaacttg
       61 acgagtaagt cagaaaatca aggtctgctt gatccttatg tgattatgaa gaaaaagaaa
      121 gactcggaaa gtttagcaat tgatattctg acttttttaa caggaatttc aagtcgagac
      181 ggggagaagt ttcctgtgtt acgtaaagcc attcgaacgg tgactcaaag tgatcaacgg
      241 gggttattac aagttattaa cgaattgcga aaagacgggt caccaatcgc ggaaaatatc
      301 gccgatcata tcgagtcctt taccgattat gattttgctc atttgctttt ctcagacggc
      361 tcgattaccc aatcgattag tttagataaa cagctaaata tcattcaagt ggcggatttg
      421 gttttaccgg atgcagaaac gacttttgaa gaatatacca ccatggaatt gcttagtgtt
      481 tcgatgctaa tcgtcatttc cacttttgcc ttggacttta tccatagtaa ccgagaaatc
      541 ttcaaaattg tcgatttgga tgaggcttgg agttttcttc aagtggctca aggaaaaacg
      601 ttatcgaata agcttgttcg tgctggtcgg gccatgaatg ctggggtcta tttcgtcact
      661 caaaatagcg acgatttatt agatgaacgt ttaaaaaata atatcgggtt gaagtttgct
      721 tttcgcagta ccgatattca tgaaatcaag aaaaccttgg aattctttgg tttggatcag
      781 gaggatcaaa gcaatcaaaa acgcctgcga gatttggaaa acg
//
LOCUS       DASNNU010000247          810 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.256, whole genome shotgun sequence.
ACCESSION   DASNNU010000247 DASNNU010000000
VERSION     DASNNU010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.256"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..601
                     /locus_tag="U8116_002854"
     CDS             <1..601
                     /locus_tag="U8116_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN8435770.1"
                     /translation="ATKLLSEKLIIAANQYKGKTETIFSCVRFGNVMGSSGSVIPLFS
                     KQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFGETYVLKMPAINILTLAQS
                     LIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIERSYENDKMYIIPPHYHATD
                     ELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            613..>810
                     /locus_tag="U8116_002855"
     CDS             613..>810
                     /locus_tag="U8116_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEN8435771.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDS"
BASE COUNT          327 a          102 c          151 g          230 t
ORIGIN      
        1 tgcgacaaag ttattgagtg aaaaattaat aatagctgcc aatcagtata aaggaaaaac
       61 agaaacaata ttttcttgtg taaggtttgg aaatgtcatg gggtcaagtg gatctgttat
      121 tcctcttttt agtaaacaaa taaaaaacaa taaagatatt actttgacag atgttgatat
      181 gacacgattt atgatgtcaa taccggatgc tgcaagatta gttctgtctg ctgctatgaa
      241 ttcaaaattt ggagaaacat atgtcttaaa aatgccggca atcaatattt taactttagc
      301 tcaatcatta ataaaaatat ataatgatac acatgatgaa aaatatagag gtgaaataaa
      361 agttgtaggt atcaggccag gagaaaaatt atatgaagag ctaatgacta cagacgaaat
      421 tgaacgctca tatgaaaatg ataaaatgta tatcattcca ccacactatc atgctactga
      481 cgaactgtac aaagattatg aaaaaattaa aatcactgca tactcttcag atgcaacaga
      541 attaatgagt ggacaggaat ttattagaat cgcaaaagag aataatgtcg tgacttggta
      601 aggagtttta tgatgaaatc agaagtatat gtaattgcag aaattggtgt aaaccataat
      661 ggaagtctag atttagcttt agaaagtatt gataaagcta aagaatgtgg agctaacgcg
      721 gttaaatttc aaacatttaa aacgaataag ctagtttcca aaaaggctcc aatggcaaaa
      781 tatcagaagg ataatcttaa agaggacagt
//
LOCUS       DASNNU010000248          786 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.257, whole genome shotgun sequence.
ACCESSION   DASNNU010000248 DASNNU010000000
VERSION     DASNNU010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.257"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            7..624
                     /locus_tag="U8116_002856"
     CDS             7..624
                     /locus_tag="U8116_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435772.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          306 a           96 c          145 g          239 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtag
//
LOCUS       DASNNU010000249          778 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.258, whole genome shotgun sequence.
ACCESSION   DASNNU010000249 DASNNU010000000
VERSION     DASNNU010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.258"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            50..664
                     /locus_tag="U8116_002857"
                     /pseudo
     CDS             50..664
                     /locus_tag="U8116_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          281 a          126 c          143 g          228 t
ORIGIN      
        1 cgtatgatta gggtgtatta taaacgttat agaaaagtag ggaataaatt tgaaaattgc
       61 ttatattaga gtttcatcca tcgatcaaaa tgaacaacgt caaattgaag aaatgaaaaa
      121 gtttggcgct gaaaaaattt ttatcgaaaa acaatctgga gcgacgatta gtcatcggcc
      181 gattttccag gaagctttag attttgtcag agagcaagat atttttattg tagaagccat
      241 tgatcgactt ggacgcaatt atgacgagat tatcgattca gtgaattatc taaagaagaa
      301 gaatgtgcga cttattatca ccagtttacc gatcatggct gaagcaattg gaaatcctct
      361 cctagataaa ttcatcaaag acttaatcat ttaaatctca gccatgattg ctgaacaaga
      421 acgtactgaa tcgaaacgac gccaggcaca aggaattaaa atcgctaaag caaacggcgt
      481 ctataaaggt cgtcctaagt tatacagtgc agacaccaaa gatcctcaac gtcgccttgt
      541 ctataggagt atcgttcagg atcttgagaa tggggttgct atctcaaaga ttgcaacaga
      601 ctacaatgtg actaggcaaa caatttaccg aataaaaaaa gaaatcgacc aactaattgt
      661 atgatttctt ttttgaatca cgtaattatt aattagcttt gtcatcaata cagacaatca
      721 ttttagattc atctagctaa gttgattaca tttatgttaa aaagaattta gttaacat
//
LOCUS       DASNNU010000250          777 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.259, whole genome shotgun sequence.
ACCESSION   DASNNU010000250 DASNNU010000000
VERSION     DASNNU010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.259"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            130..777
                     /locus_tag="U8116_002858"
                     /pseudo
     CDS             130..777
                     /locus_tag="U8116_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          264 a          137 c          154 g          222 t
ORIGIN      
        1 atctcctata aagttgtcaa aattctcgga gtggccgatt attatatcgt cataacggtt
       61 ctgttgcaaa gttttccaaa aaatctattt tagtgtaaaa ttgagaaaaa agacagagag
      121 gacagagtaa tgaatcattt taaaggcaaa caattcaaaa aagacgtcat tattgtcgct
      181 gttggttact acctgcgtta caatctaagc tatcgtgaag ttcaggaatt gttatatgat
      241 cgtggaataa atgtttgtca tactacgatt tatcgttggg tataagagta cagcaaagtc
      301 ctctattatc tttggaagaa gaaaaataga caatccttct attcatggaa aatggacgaa
      361 acctatatca aaattaaggg acgttggcat tatctttatc gtgcaattga tgcggacggc
      421 ttaaccttag atatctggtt acgaaagaaa cgggatacgc aagcagccta tgctttctta
      481 aaacgactcc ataaacagtt tggtgagccg aaagcaattg tgaccgataa agcaccttct
      541 cttggctccg cctttagaaa gttacagagt gtgggtttat atactaagac agagcaccga
      601 actgtgaagt atcttaacaa tttaatagaa caagaccatc gacctattaa acgacgaaat
      661 aaattttatc aaagtctccg tacagcctct tccacgatta agggcatgga gaccattcga
      721 ggaatatata aaaagaaccg aagaaatgga acgctcttcg gcttttcggt gtctact
//
LOCUS       DASNNU010000251          768 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.260, whole genome shotgun sequence.
ACCESSION   DASNNU010000251 DASNNU010000000
VERSION     DASNNU010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.260"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          258 a          113 c          116 g          281 t
ORIGIN      
        1 taacttttat tatagaaggt attgtaaata ataatattga caaaaagtga ctcaaaataa
       61 aaaaaacctt acattatact ggaatgaact gtattgatag tgtacctaaa agctctactg
      121 acataagatc gtgtaatcgg taaagtaatt ttattaatta tacgggatgt aatcaattag
      181 agaataaact aatcataaaa ggtactttta cattcataat tgtcgctttg agattctact
      241 ttggttcact atctgaccca attgcacatt ttcgatcatt acaagtattg gtgtcgttag
      301 aacaactaaa taaaaactag cggtagattt ttcagaatct atgctaattt ctgtatggat
      361 gttccagtga tggcaaattt ctcttttcat tgtttttctt gtcgcatgat atgccttttt
      421 gtgttattca gttaaactat agaaattctt tcctagacag atcttgatcc caaattttac
      481 gtttttgagc atcgatatag tttaaaatga cattactcat atctaaagag tttagatttt
      541 ttaggactaa aactgtcagt tgttcttttt cttctttctg ttaaagtatt gtcgcgactt
      601 ttcgtatacg ttaagatatg tcgtgaaaaa acaagttttg tgacacaaaa aaacgaatga
      661 cttgaggaaa tcacctcaat cattcgttta aaatgctata tattctttag tttaagatac
      721 ggaaattaat gccatcgacc acactagtag aatgaacgac atttactt
//
LOCUS       DASNNU010000252          768 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.261, whole genome shotgun sequence.
ACCESSION   DASNNU010000252 DASNNU010000000
VERSION     DASNNU010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.261"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..212
                     /locus_tag="U8116_002859"
     CDS             <1..212
                     /locus_tag="U8116_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN8435773.1"
                     /translation="ICTGVLAMIPICLITKMMGKKLIYIESFAKVTSPTQTGKLMYHF
                     ADQFYVQWETMLQFYPNAIYLGGIY"
     gene            229..708
                     /locus_tag="U8116_002860"
     CDS             229..708
                     /locus_tag="U8116_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HEN8435774.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
BASE COUNT          288 a           88 c          151 g          241 t
ORIGIN      
        1 tgatttgtac aggtgtttta gctatgatcc ctatctgctt aataacaaaa atgatgggaa
       61 aaaaactgat ttatattgaa tcgtttgcaa aagtaaccag ccctactcag acaggaaaat
      121 tgatgtatca ttttgcagat caattttatg ttcagtggga aactatgcta caattttatc
      181 ctaatgctat ttatttgggt gggatatatt aaataatagg agaaaaaaat gatatttata
      241 acattaggtt cacaaaaatt tcaatttgat agattgttaa aagctattga tgatttaatt
      301 gaaaaaggag taataatcga aagtacattt gcacagagtg gttatagtga ttataaacca
      361 ctaaattatt cctataaagc atttctaaat agggatgagt ttactgaaac tatgcaaaaa
      421 gcagaaatgg tgattactca tggaggtaca ggtgctataa ttggggccgt aaaaaaaggt
      481 aaaagggtca ttgctgttcc tcgattggct aagtatggtg agcatgtcga tgatcaccaa
      541 ctgcagttaa ttagacaatt tgatgagatg aatattatct atccttgtta tgaattagaa
      601 aatttagaag atgccgttaa agaaacaaga gttcggatgt ttaacaagta tgtatctaac
      661 acggaaacga ttattaattc gatagaggta ttaatagaaa aaatataaat ataaggagta
      721 aagcgttatg caaagagtac ttatgattgg taatgacagc tcggtaaa
//
LOCUS       DASNNU010000253          763 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.262, whole genome shotgun sequence.
ACCESSION   DASNNU010000253 DASNNU010000000
VERSION     DASNNU010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.262"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            14..727
                     /locus_tag="U8116_002861"
     CDS             14..727
                     /locus_tag="U8116_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435775.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT          250 a          150 c          137 g          226 t
ORIGIN      
        1 tgaggtattt tatatgaaaa agttaggttt tattttattg ttggtctctt tattccttgt
       61 aggatgtacg tctcctagta aagaaaatag tgatccatct gcgaccacag acaaaagttc
      121 aatcagacaa accagttcag ctgaaaaatc aagtactcag aaatcagctt ctactgaaac
      181 gaatacatct tcaacaagca gtaaagtctc agagcccaat acttcttcta catctagtag
      241 tacagttgag acaaatattt cttcttcaac aatagacaca acctcctcac agtcttctca
      301 gtcaatagat ccgttagcag gatattctga tctacaaatt gagtacgcac gtgtttggct
      361 agctattaga ggtaatactt ttagagatac atcgggtgat ttcgaattac atgttattca
      421 ttatcctgca ggaacaccta tcgcccctta tgatgcaggg agtgcagtct atccgaaaga
      481 tgtggttatg ctcactggga aatacagtta ccagggagtt ttagtgtatt caagtaacca
      541 tgacgggaca atcactagtt atccagttcc ttcacattgg caaatgcctg cagatcaaac
      601 atcttatcca gcgttcatta gagaaacaac tcaagaaatc attgataatg catctgtagt
      661 agctattcct acaggaaatc ccaatgacgt gaaacagtta atcgatgtga cagttataga
      721 taattagact ttctaatatg ttatacaaaa agaagctgac agc
//
LOCUS       DASNNU010000254          754 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.263, whole genome shotgun sequence.
ACCESSION   DASNNU010000254 DASNNU010000000
VERSION     DASNNU010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.263"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(347..547)
                     /locus_tag="U8116_002862"
     CDS             complement(347..547)
                     /locus_tag="U8116_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN8435776.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          275 a          119 c          116 g          244 t
ORIGIN      
        1 ttgaaacatc catctggaat ttgtcttttt cgttgctttg ctcataaaaa tctgcatatt
       61 catcactaat attttttcta ataaattctt ccatcataaa gatcacctcg aaaagagtat
      121 acgaacaaac gttctttttg taaaggtgga atttattata ctaaataaat aggtgaacaa
      181 gtacttgtgc caaattatgt gccaaaaaaa atcgaattta ataaaacaca aacaaaaaga
      241 aatcctatta tgataacatt tcttataata acaaacacaa cagaagacgt gtaatagtta
      301 gtcaggaacg tacaaataac ccctgtatcc tttgcggtac agggggttat tttgtataag
      361 tagacattgt agtttctaaa ctgctatgtc ctaaacgttt cgatacgcta agtatattta
      421 ctccttgata aagtaaaata gatgcatgcg tatgtcttag tccatcaata aacagtcgaa
      481 gagaaaatag ggaaaaattg attactaaag aaaaacttac taggtcgacg gtgtagttga
      541 ttttcatact tcaatagatt cgaattaaac gatttcgatt ggcttatacc agtcctatcc
      601 aacgaaagat atatcgttct taactctagc ggtataactt gaagctttac gtaaagccaa
      661 tgggaatgaa ctggagatga ctttttatta tttttagtaa tagctgatgg gttaatactt
      721 acaactttcc ttttcaaagt accatataaa aaaa
//
LOCUS       DASNNU010000255          742 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.264, whole genome shotgun sequence.
ACCESSION   DASNNU010000255 DASNNU010000000
VERSION     DASNNU010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.264"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..657
                     /locus_tag="U8116_002863"
     CDS             <1..657
                     /locus_tag="U8116_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN8435777.1"
                     /translation="QLKRKELVYLKKYTDSLEKDQEKFARFRHDYKNLLLSLKEIANQ
                     NHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKICCKF
                     ECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSC
                     REILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF
                     "
BASE COUNT          305 a           85 c          122 g          230 t
ORIGIN      
        1 caattgaaaa gaaaagaatt ggtttatcta aaaaaatata ctgattcact ggaaaaagat
       61 caagaaaaat ttgccagatt tagacatgac tataaaaatt tattgctgag tttaaaagaa
      121 atcgctaatc aaaaccatga ttcgatattg gaaaaagaaa taaaagaatt agagatctat
      181 tctaattctt atctaaccaa taagtttgaa tatcgttatt taaaaaatat aaaaaatgaa
      241 tatttgaaaa gtttgttgat agcgaagcta taccaagcaa ataacaataa gatttgttgt
      301 aaattcgaat gtccaagtac tattattgaa gttcctgttc caatatttga ctgtattagg
      361 gtgcttggaa ttattctcga taatgcaatt gaagcatcta tagagagtca agatagagaa
      421 ctgtctctag ctatttaccg agatgataaa cagatagaaa tttcgatatc gaatagttgt
      481 agagagatac tactctctat agatactcta atgaaaaaag gaataacaac aaaaaaggga
      541 catcaagggt taggtttatc tagtatagag gaaatcaata aaaagaatcc caatatgttt
      601 gtgaactatc aaaacgatcc gtatcaattt actactcaag ttattttgtt gttttgaggg
      661 aaggaggatt tatgagttat ccaataatat tatgtgaaga tcaaattata caattaaatc
      721 agttagaacg aattattgat aa
//
LOCUS       DASNNU010000256          740 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.265, whole genome shotgun sequence.
ACCESSION   DASNNU010000256 DASNNU010000000
VERSION     DASNNU010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.265"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>740
                     /locus_tag="U8116_002864"
     CDS             <1..>740
                     /locus_tag="U8116_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN8435778.1"
                     /translation="ILGQIVLNLMCAVVSQKMYPDYTPVGELEKETIDDIIKKVKGLV
                     TNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIMSIFAIIYESCIAGIGNSL
                     IVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMIIWVGSDNLMSISFVIFMIV
                     HFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNIILVNWFGLYGVLAATIVS
                     VLLVDLPWLLYNVFQNIFKFESAGKYVW"
BASE COUNT          230 a           85 c          135 g          290 t
ORIGIN      
        1 ctattttggg acaaatagtt cttaacttaa tgtgtgctgt tgtttctcaa aagatgtatc
       61 ctgattatac tcctgttggt gagttagaga aagaaactat tgacgatatt ataaaaaaag
      121 taaaaggatt agttacaaac aaggtaggcg gtacaatatt acgatcttca gattcaatta
      181 ttatttcatc tttcttaggt ctttcagtac ttgcgattta tcagaattat tactttatta
      241 tttctgccat aatgagtata tttgcaataa tttatgaatc atgtatagca ggtataggta
      301 acagtcttat tgtagaaagt caagaaaaaa attatataga tttttgtgat attacaacaa
      361 ttataggttg gtttctggca attagttgtg catgctttat tacattattt cagccgttca
      421 tgattatctg ggtgggaagt gataatctta tgagtatttc atttgttata tttatgatag
      481 ttcatttttt cttatatgaa attgatcagt taattgggtt atacaaagat gctgctggaa
      541 tatggtacgc agacaggttt agaccattaa ttacagcaat gataaacatt gtattaaata
      601 tcatcctcgt taattggttc ggattatatg gagttttggc ggcaaccatt gtttcagtat
      661 tgttggttga tttaccctgg ttattatata atgttttcca aaatatattt aaatttgaat
      721 cggcaggtaa atatgtttgg
//
LOCUS       DASNNU010000257          736 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.266, whole genome shotgun sequence.
ACCESSION   DASNNU010000257 DASNNU010000000
VERSION     DASNNU010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.266"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(72..293)
                     /locus_tag="U8116_002865"
     CDS             complement(72..293)
                     /locus_tag="U8116_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435779.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(391..636)
                     /locus_tag="U8116_002866"
     CDS             complement(391..636)
                     /locus_tag="U8116_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435780.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          225 a          140 c           96 g          275 t
ORIGIN      
        1 atttattgta atatgaagct gtattttaca tatagtaacc aaagagttaa taaaataaaa
       61 agagcgaacc attatagatc agctcttttt tcattgctgt ttttaatctt atctagaatc
      121 acttcaaatg tttcaaaggt atttacatct aactgacata accaaagcaa gtacttcttt
      181 tctttgtaag tccaatcaat attacggtaa gaggacatta gattatggta ccttgctctt
      241 tgttcaactg aagccaattc ccagcgtttt tcaaatattt tttcttcttc cattattgat
      301 ccctcgcaag acgctacctc aaacttaaat aatgggcagc gaaagtctaa ttccttagat
      361 ctaagtatag caaacaaata tgaaaaaaac ttagaaggta gtaaaagttc ttccgtcttt
      421 tgctttgctt ctattcttaa tgtgtttcgt tatcggtgcc ctacaaagat tttcattaat
      481 tacacactca tttattgttg tgtattttct gacatttctc tcttgatcaa ttattttaat
      541 tccatgggca tcctgatagt tcataggaga ggagcggcta tcagaaatag ttgcacctat
      601 ctcttgaact tcttttacaa cttctggaaa atccatccat cctgcttcag ctgtcaaaag
      661 taataatcta taatatttat ctttcctttt caatctaaga aatcgtcacc ccattctttt
      721 tcttctatta taaact
//
LOCUS       DASNNU010000258          710 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.267, whole genome shotgun sequence.
ACCESSION   DASNNU010000258 DASNNU010000000
VERSION     DASNNU010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.267"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>710
                     /locus_tag="U8116_002867"
     CDS             <1..>710
                     /locus_tag="U8116_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435781.1"
                     /translation="YVSSMITGQGSAGGYVVLWPKEKPSNIFIMDNPDLEKAREVLRM
                     NKNGIAFSKKGWRGPFNSAWTLDSIFNANFIQTGLIKADIFQNSFNKTGDVLKLVNGL
                     LQIWNNKKKIMELTKKGMEFWNSKGSIGTIGTTDSVGNPFPGASTPTPIEDNSLVIRT
                     NGDGKYILISPKVGKGLVLLGNGKAIYFGDLDVQGKLTVKGKEIIPGQGGGPSGGGES
                     TGGYPSELKTDAEKRAWR"
BASE COUNT          257 a           89 c          153 g          211 t
ORIGIN      
        1 tatgtttctt caatgattac aggtcaaggc agtgcaggag gatatgtagt tttatggcca
       61 aaagaaaaac cttccaacat ctttattatg gataatccag atttagaaaa ggcaagagaa
      121 gtactaagaa tgaataaaaa tgggattgct ttttctaaga aaggatggcg ggggcctttt
      181 aattcagctt ggacattaga tagtatattt aatgccaatt ttatacaaac tggattaatc
      241 aaagcagata tctttcaaaa ttcttttaac aagactggag atgtattgaa attagttaat
      301 ggactactcc aaatttggaa caacaaaaag aaaataatgg agttaactaa aaaaggaatg
      361 gaattttgga attctaaggg ttcaattgga acgattggaa caactgattc tgttggtaat
      421 ccttttcctg gggcttctac tccaacccct attgaagata attctttagt tattcgtaca
      481 aatggagacg gcaaatatat tttgatttct cctaaagtag gaaaaggatt agttttatta
      541 ggaaatggta aagcaattta ttttggagac ttagatgtac aaggaaaact tacagttaaa
      601 ggaaaagaaa taattcctgg acaaggtggt ggtccatcgg gaggtggaga atctactggt
      661 gggtatccta gtgaattaaa aacagatgca gaaaagagag cttggagaat
//
LOCUS       DASNNU010000259          682 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.268, whole genome shotgun sequence.
ACCESSION   DASNNU010000259 DASNNU010000000
VERSION     DASNNU010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.268"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..652
                     /locus_tag="U8116_002868"
     CDS             <1..652
                     /locus_tag="U8116_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEN8435782.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     AYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDH
                     RPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          232 a          120 c          136 g          194 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt
      361 agtcacagat aaagccccct ctattacaag tgcctttaag aaactaaaag aatacggctt
      421 ttatcaaggg acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca
      481 tcgtccagta aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccacgat
      541 taaaggcatg gaagccattc gaggattata taagaaaacc cgaaaagaag gcactctctt
      601 cgggttttcg gtctgtactg aaatcaaggt attattggga atcccagctt aaatcataga
      661 taccgtaagg gattttattc tt
//
LOCUS       DASNNU010000260          662 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.269, whole genome shotgun sequence.
ACCESSION   DASNNU010000260 DASNNU010000000
VERSION     DASNNU010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.269"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..448)
                     /locus_tag="U8116_002869"
     CDS             complement(<1..448)
                     /locus_tag="U8116_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8435783.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          195 a          119 c           90 g          258 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gc
//
LOCUS       DASNNU010000261          656 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.270, whole genome shotgun sequence.
ACCESSION   DASNNU010000261 DASNNU010000000
VERSION     DASNNU010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.270"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..461)
                     /locus_tag="U8116_002870"
     CDS             complement(<1..461)
                     /locus_tag="U8116_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435784.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFI"
BASE COUNT          273 a           99 c          110 g          174 t
ORIGIN      
        1 tgaataaaag gaattaagaa tcccgcactt aaaaaaaagg tgaggacggt agcttttaaa
       61 agacttaaga gtctagcgat tttttggtcc caaaaataaa aagaaagaaa caacgttcct
      121 ccaataaata cagaaaccat agcgacagaa agtaaatgag tatacactac tagcgtcatg
      181 ccaatagaca accagtacca tttttggtaa tctctaatat aaatttcgta acaccccatt
      241 agtatcaaag gcaaaaatgt taaagcgaca gcttctccca atgaagcacg atggaatatg
      301 tcaatggcac gataggcgga aaatgtataa ataatcgaaa ataacagcga aatataacga
      361 ttgtttttta tttgtttcat agaaaaataa gaaacgacca ttgttaaaaa agtgataaaa
      421 aaatagtaga tattataact taaaacaaga tttcccatca ttttataaaa taaaaaagct
      481 ggataaagag taagccatgg ataaaaaata ttcatcattg ttccatgatg atcgaagtta
      541 ttgaagttta ctggagaact ccatacatta tctagtccta tgattcgaga taaatgaaag
      601 taagtatcct gattgacact atgatagatt cccttagaca tgaatagagg gatgac
//
LOCUS       DASNNU010000262          653 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.271, whole genome shotgun sequence.
ACCESSION   DASNNU010000262 DASNNU010000000
VERSION     DASNNU010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.271"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>653)
                     /locus_tag="U8116_002871"
     CDS             complement(<1..>653)
                     /locus_tag="U8116_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEN8435785.1"
                     /translation="SLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKI
                     DNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKN
                     YSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEI
                     GKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYN"
BASE COUNT          218 a          108 c           76 g          251 t
ORIGIN      
        1 taattataaa tcgtaatatg catttcattc ttaaagcttt ctcttaattt agtttcactt
       61 acagaaaaat aattagctat ttcctttaca gtaattcttt tatagatatt taagccgatg
      121 aattgaacga cagaagaaat taagggagaa agatgctcta tcttagtttt tccgatttcc
      181 ttagtataat aaacaatccc agaatccctc acttttaaga cctcaggtaa cgatacagct
      241 aattcgtttt cttgtatcaa agcatctcta ctttgatacg cttctattat atccattcct
      301 aatgttatag acgtttgtgc taacttttca aaaacaatga tagaataatt tttttcagac
      361 cgaatcgtat ctccactaac acttggaatt attccattac tcagattaaa aaccatgttt
      421 tctaaatttc gaatatctcc atttttaaca tattccagta ttttattttc ataaagaaac
      481 agatatattt ctggatcgta attttgtgat aacagattat ctattttgat ttgctcgata
      541 gttttactta gattttctgt atattcaatt aacggttcat ggaatagatc ttctattttt
      601 ccagtaaaaa aataatgaac taaaatcaga atatctcgaa tgtctcctaa aga
//
LOCUS       DASNNU010000263          652 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.272, whole genome shotgun sequence.
ACCESSION   DASNNU010000263 DASNNU010000000
VERSION     DASNNU010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.272"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..242
                     /locus_tag="U8116_002872"
     CDS             75..242
                     /locus_tag="U8116_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEN8435786.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(302..>652)
                     /locus_tag="U8116_002873"
     CDS             complement(302..>652)
                     /locus_tag="U8116_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8435787.1"
                     /translation="RKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTNI
                     ENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLME
                     SLASGINEARNIED"
BASE COUNT          215 a          104 c          117 g          216 t
ORIGIN      
        1 atgaaatatt ttttaataaa gatttgaatt aaagtgtaaa ggaggagata gttattataa
       61 actacaagtg gatattgtgt cctgtatgtg gaaataaaac acgattaaag ataagggaag
      121 atactgaatt aaaaaaattc cccctctatt gtccgaaatg cagacaagaa aatttaattg
      181 aaataaagca gttcaaagta actgtgatta cagagccaga cgcaaagacg cagagccgat
      241 aaaatgagat taatacaatc tcattttatc ggctctttcc gttatgtatg gattctttta
      301 attagtcttc gatgtttctt gcttcgttga taccgctggc taaagattcc attaaggata
      361 gttctttgtc tgtaaagcta tccatgtatt tctctatctg taatcgtcgg gtgcttttta
      421 ccaagttatt agcaggtaag aaaaattcat caacggaaac atgaagtaac gatacaaggt
      481 cataaagaac ttgtatgctg gggtgttgcc ctttattttc aatattagtt aagtaccgtg
      541 ggtcaatttc aatcaatgct cccacttgtt cacgagttaa acctcgtttc aatcgagctt
      601 ctttaatggc taaaccaaag gctctaaaat catatttatc ttctttttta cg
//
LOCUS       DASNNU010000264          647 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.273, whole genome shotgun sequence.
ACCESSION   DASNNU010000264 DASNNU010000000
VERSION     DASNNU010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.273"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..326
                     /locus_tag="U8116_002874"
     CDS             <1..326
                     /locus_tag="U8116_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEN8435788.1"
                     /translation="NISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFT
                     KKYWNEMENLKKYIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKK
                     ELLGL"
     gene            360..>647
                     /locus_tag="U8116_002875"
     CDS             360..>647
                     /locus_tag="U8116_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEN8435789.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWG"
BASE COUNT          267 a           84 c          107 g          189 t
ORIGIN      
        1 aaaatatatc agccgtacaa ttaaaaaaac caggtatgag taatggttat atacaattta
       61 ccttgctagg cggaaacgaa agtcgcggag gtatattagc ggccacgaaa gatgaaaaca
      121 ccgttatgtt tacaaaaaaa tattggaatg aaatggaaaa tttaaaaaaa tatatagaga
      181 agcaacaatc tatattagat aataattcca aagaaacaca aatttcaagt gcagatgaaa
      241 ttaaaaagta taaagagttg ttagatgaag ggattattaa tcaagaagaa ttcgatttta
      301 agaaaaaaga attgcttggt ttataatatt caatatcaaa agagaaagag gatctattaa
      361 tggacggtat atttggtttt actggtattg ttttatttat tataggttta atcatgttaa
      421 ttgtaagatt tatcaaaaaa acaaataaga aaacacctat gattctcctt attatcggga
      481 ttatttttac taccgtaggt ttctcgcttt cctcatcaaa cgaggcaaat gacaataaca
      541 aacaggaaag ctcatcatct gatactcaaa ctactacatc taacagcagc aataaaagcc
      601 taactcaatt tgaagaattt ttagagacaa ataatcatga ctggggt
//
LOCUS       DASNNU010000265          634 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.274, whole genome shotgun sequence.
ACCESSION   DASNNU010000265 DASNNU010000000
VERSION     DASNNU010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.274"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            209..607
                     /gene="spxA"
                     /locus_tag="U8116_002876"
     CDS             209..607
                     /gene="spxA"
                     /locus_tag="U8116_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN8435790.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          226 a           96 c          120 g          192 t
ORIGIN      
        1 ttgaaaactc taatttttaa atgataaaaa aagcgagaat gctggtttta ctgtatatta
       61 accttagata actttcattt ttttaaaaaa attatgaaaa aaaggtagct atttaaaagt
      121 tgattatgta taatagaaac agatgggaga ttattatgat cttctgttac aaaaaagtga
      181 ttcttatcac gaaaaggagt ggtgcacgtt gttaacatta tatacttccc caagttgtac
      241 ttcctgcaga aaggctcgtg cgtggttgca agaacatcag attccatttg ttgaacgtaa
      301 tattttttca gaaccattga acagttctga attaaaagcg attttacaaa tgacagaaga
      361 cggaacagaa gaaatcatct cgacacgttc caaagtattc caaaaactga acatggattt
      421 agatgatctg ccgttacaag aattattgga acttgtgcaa aacaatcctg gattgcttcg
      481 ccgtccaatc atgattgatg ataaacgtct gcaagttggt tttaatgaag atgaaattcg
      541 tcggttccta ccgcgcgatg tacgacaatt agaattacgt caagcacaat tgatggctgg
      601 attatagaag gataatacga aacttggaaa agac
//
LOCUS       DASNNU010000266          628 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.275, whole genome shotgun sequence.
ACCESSION   DASNNU010000266 DASNNU010000000
VERSION     DASNNU010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.275"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..>628
                     /locus_tag="U8116_002877"
     CDS             22..>628
                     /locus_tag="U8116_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN8435791.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEEEF
                     GHLEGDTIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIE"
BASE COUNT          224 a          117 c          130 g          157 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattcaaa agaaatattt ctgaacgtaa aaaagattat
      481 gtcgttttcg aagaagaatt tggacattta gagggtgata cgattgtcgg catccaccat
      541 aaaagtgccg tcatcacact cgttgagcga ctttcaaaag ccattatcgc cttgaaacca
      601 gaaggccgta aggcagttga tattgaaa
//
LOCUS       DASNNU010000267          593 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.276, whole genome shotgun sequence.
ACCESSION   DASNNU010000267 DASNNU010000000
VERSION     DASNNU010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.276"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..580
                     /locus_tag="U8116_002878"
     CDS             <1..580
                     /locus_tag="U8116_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN8435792.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DASNNU010000268          588 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.277, whole genome shotgun sequence.
ACCESSION   DASNNU010000268 DASNNU010000000
VERSION     DASNNU010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.277"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..383)
                     /locus_tag="U8116_002879"
     CDS             complement(<1..383)
                     /locus_tag="U8116_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435793.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSE"
     gene            complement(400..>588)
                     /locus_tag="U8116_002880"
     CDS             complement(400..>588)
                     /locus_tag="U8116_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN8435794.1"
                     /translation="YISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAG
                     VSPKQYQLGLVDNFNNIS"
BASE COUNT          209 a          107 c           61 g          211 t
ORIGIN      
        1 ttttctgaaa aagttagacc cacgtaataa tcttcccagc tttctctttc aattaactga
       61 tccaattttt gataatccat aaattcatca tgtatcaaaa agaataaata acttgatatt
      121 ttagcatcta gcttaatcag attaaataat gtttgtttaa aattgctacc attaatatga
      181 ctgatagatt ctctagcttc ttcacataaa ctttttactt tattaattac tactttgtca
      241 tattcattta acacactatt attaccatta cttagacgct cagattctac gaaacaactc
      301 aaatcagaaa tttcccaaaa aaattttttg ttaaaatcca atttcctcaa ctcctccaaa
      361 ctacgtaaat gatatagtct cataataaaa taaaacctct cagctaatgt tgttgaaatt
      421 gtccactaaa cctaattgat attgcttagg agatacacct gcgtactttt tgaatactct
      481 cgaaaagtgt gaagaatctg aaaatcctaa acctaaagaa acttcactga tagtatggtt
      541 tgatttcaac attattttag ccgtagaaat ttttcgctta gagatata
//
LOCUS       DASNNU010000269          582 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.278, whole genome shotgun sequence.
ACCESSION   DASNNU010000269 DASNNU010000000
VERSION     DASNNU010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.278"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..>582)
                     /locus_tag="U8116_002881"
     CDS             complement(37..>582)
                     /locus_tag="U8116_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN8435795.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          166 a          110 c          100 g          206 t
ORIGIN      
        1 aatccactcc ttttttcatg ctctaaaatg atatttttac acaactgtta cggttgtttt
       61 acgaaattgg acgggacttt gccagtttaa tttctgtttt atccgttcat tattgtagta
      121 atagatatag ctatcgatgg ctgattttaa ttcctcgaaa cagttataaa ctttcccgtg
      181 aaaaatctct tgttttaata agccaaaaaa attctccata agtgaattat ctaagcagtt
      241 ccctttccga gacatgcttt gaaagatttt gtactccttc aatttgtttc tataagcttt
      301 catttgatac gcccaacctt gatctgagtg aaacgtacga cgatagggac aatcgttagt
      361 ggtctggata gcttcctcca aaccttccat taccgctaat gcattgggct tttcagagat
      421 acgataggat aaaatctcac tattgaacat atccataaaa ggatctagat acaatttctt
      481 ttgacggata atgcctttgg catccacttc atggtattta aattcagttg tatccgtcgt
      541 gattttctga tggcagatac tggtgtagaa acgacgatgg at
//
LOCUS       DASNNU010000270          579 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.279, whole genome shotgun sequence.
ACCESSION   DASNNU010000270 DASNNU010000000
VERSION     DASNNU010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.279"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            123..539
                     /locus_tag="U8116_002882"
     CDS             123..539
                     /locus_tag="U8116_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435796.1"
                     /translation="MQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPE
                     IHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSKYNLLEYD
                     LIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
BASE COUNT          190 a          113 c           85 g          191 t
ORIGIN      
        1 attgcaaaga aaaaaacgaa gaatatgttg ttcaattatt aacgcaaatt tattttttct
       61 cccgtatttt cccatacgat acgactcatt tgacgatccg tgcaacttac tttgcacagc
      121 agatgcaaca ccgccagata cgtctatatg ctggatttcg tgcagaactt caattttatt
      181 ccactctact gaatcagaat ctgatttatt atttatctag ctatatcttt tggatgctta
      241 tcggtaatcc tgaaattcac cattatactt ctgataaaaa aatactgatt atcagcgact
      301 taagtcttcg acatagtcaa tatattgaag aatatatcag tgatattctt gctgtgcata
      361 aaattcactc tgagacaact gctattactg aagatcagct gtcgaaatac aatcttctcg
      421 agtatgatct gatcgttacg aatcaaccca ttttgaatgc ccatgttcct catatattga
      481 ttgatgatag tgtgtcattc gcaaatgaag aagaactcat ccgcttattc gagttatgaa
      541 taatcaaaaa acagctatgt ataaaccacc ctgataaaa
//
LOCUS       DASNNU010000271          574 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.280, whole genome shotgun sequence.
ACCESSION   DASNNU010000271 DASNNU010000000
VERSION     DASNNU010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.280"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>574)
                     /locus_tag="U8116_002883"
     CDS             complement(<1..>574)
                     /locus_tag="U8116_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEN8435797.1"
                     /translation="NKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVYAF
                     DVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLKKS
                     LSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIKLV
                     DKNNQAITKEYLGKVREYISKFNEFTNMDIL"
BASE COUNT          179 a          109 c           70 g          216 t
ORIGIN      
        1 aagaatatcc atattcgtaa attcgttgaa ttttgatata tactctctta cttttcctaa
       61 gtattcttta gttattgcct gattattttt atcgactaac ttaatgattg atccaagttc
      121 tttcccattt tcaaattgat caaaaattaa ctctttgtat tcattccagc gttcactata
      181 cttatcattg ttcaattcac taatacgctc ttgatactcg tctgtcactt taggagaatc
      241 aataaactct cttaaattcg gcgatagact ttttttcaaa aaaagacgat cgtattgacg
      301 ccctttcatc ataattgcaa aataagacaa ttgatacgct cttttatcga tttcaagacc
      361 atataaattg tattcaacga ttgcacgtgc agcatcacgc tcagaatatc ctaacgtaag
      421 ataaatttcc atcaatacat cgaaggcgta aactaaaata tgcccagacc ccattgcgtt
      481 atcgataatt tttagttctt ctggttttaa attacttaca gatgttgctt gttcaaaagc
      541 atatttatct ggaattaagt atttcaactt attt
//
LOCUS       DASNNU010000272          574 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.281, whole genome shotgun sequence.
ACCESSION   DASNNU010000272 DASNNU010000000
VERSION     DASNNU010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.281"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            28..>574
                     /locus_tag="U8116_002884"
     CDS             28..>574
                     /locus_tag="U8116_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435798.1"
                     /translation="MEIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYP
                     WLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTT
                     SSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLS
                     VAMTMVMIIIALIIQFYRKKKI"
BASE COUNT          175 a           85 c           81 g          233 t
ORIGIN      
        1 ttatatttat ttccagcatt taaaaacatg gaaatatttg gcttttatga tacggcattt
       61 cacattaatc gtgcgttaag tttagaatct atttttagct cccctattaa ttttgaaacg
      121 tttagatcat atggcatgca agtaaataat ttttacccat ggttaactct atatccttta
      181 tttttattga tcaagtttac taatttagca ataggctata acctttttct ttatattgtt
      241 acgctcatta ctctatttat atgtcattat gtaatgtatg aaattaccaa aaaacatgtt
      301 actagctcct ttttcgctat tatatataca acatcttctt ttaggagtgt agaaatattt
      361 cttcgagggg caatggggga attactcgca atgtcaatac ttcctcttat tttactcggg
      421 tttataaagc tttacgactc aaaaaaagaa tcttgggtta tgttggcgat tagcatgact
      481 ttattaattt acactcatgt tctttctgta gctatgacta tggtaatgat tataattgct
      541 ttaattattc aattttatcg gaaaaagaaa atag
//
LOCUS       DASNNU010000273          570 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.282, whole genome shotgun sequence.
ACCESSION   DASNNU010000273 DASNNU010000000
VERSION     DASNNU010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.282"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..437
                     /locus_tag="U8116_002885"
     CDS             <1..437
                     /locus_tag="U8116_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEN8435799.1"
                     /translation="SYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGV
                     RFYHTQGIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKI
                     ANNVVVAAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
BASE COUNT          197 a           60 c          128 g          185 t
ORIGIN      
        1 catcttatta ttattcaaag agaaagaaaa atatcattta tgcgattatg tatattgtgt
       61 cttgcagaag aatgaatagt ttgggaagaa aatgtggtat agagtgtggt gaaaatgtat
      121 ttgatgaagg tgtaagattt taccatacac aaggaattgt tattaatggt aacgccataa
      181 taggaaaaaa ttgttactta tatgggaata actgtattgg aaatgatgga atcgatccta
      241 agtgtcctgt aattggaaat aatgtacgtg tatgcgtggg tgcaaagatt attggtaatg
      301 taaaaatagc taacaatgta gttgttgcgg caggagctat tgttatcaaa gattgtctag
      361 agaatggtgc aattcttgca ggtgtaccgg caaaaataat agggtatgtt aatttaacaa
      421 aagtattttt agaataattt ttagcgaaag aaggtgaata acgatgcgta tttcaataaa
      481 aagatggtca ggagcagatt ggacattgtt cacgttggca ttttttgcaa tctccatagt
      541 tcttgtatat acttttaacg ccccaagagc
//
LOCUS       DASNNU010000274          567 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.283, whole genome shotgun sequence.
ACCESSION   DASNNU010000274 DASNNU010000000
VERSION     DASNNU010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.283"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            168..362
                     /locus_tag="U8116_002886"
     CDS             168..362
                     /locus_tag="U8116_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435800.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          238 a           72 c           90 g          167 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatg
//
LOCUS       DASNNU010000275          557 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.284, whole genome shotgun sequence.
ACCESSION   DASNNU010000275 DASNNU010000000
VERSION     DASNNU010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.284"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..440)
                     /locus_tag="U8116_002887"
     CDS             complement(<1..440)
                     /locus_tag="U8116_002887"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEN8435801.1"
                     /translation="MISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELAT
                     IVFSSSQLAVSHETPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNK
                     NGSILVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQ"
BASE COUNT          208 a           97 c           85 g          167 t
ORIGIN      
        1 atttggatca accttccaga gacatcaatc aaaggaaatt tcttaagata attcagattt
       61 atttttatcc ctctgccaaa ctgttcattt tcttcattca agtaatctga tacattgact
      121 aatatacttc cgtttttatt caaagtttca tataattcct gatcggctgc tgatgcaaaa
      181 cgattggaat aaataaaatc cacgtggttt ttcccgatgg ttactggata aaatacatga
      241 tagtcctcac cagcgatttc ttttggtgtt tcatgggaaa cagctaactg tgaagaagaa
      301 aaaacgatag ttgctaattc gacattggca acggtcaaaa gaagagaaaa caacaatcca
      361 aatgtaaaaa gaatgtattg gatatagttg aacgcttctc ttaacgaagc gattttttca
      421 actaataaaa gagaaatcaa agagataccc tatattccgc tgaacaagaa aacgtacatc
      481 tggaacaaat aaagaaattg ctccttataa tacataaaaa taaaaccacc aatgattcct
      541 gcaattatga atgtaat
//
LOCUS       DASNNU010000276          555 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.285, whole genome shotgun sequence.
ACCESSION   DASNNU010000276 DASNNU010000000
VERSION     DASNNU010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.285"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            365..>555
                     /locus_tag="U8116_002888"
     CDS             365..>555
                     /locus_tag="U8116_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435802.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSST"
BASE COUNT          199 a           59 c           80 g          217 t
ORIGIN      
        1 aaaaagtgag aaaaaaggct tataattagg atttagagta attaattaga tgtttatttt
       61 ataggactga tattatagat tacaaatagt atcgtatttc gcaatcttaa aattatcttc
      121 ttactatgaa taagataata aaatttgtat ttgtcctttt taggtaactt attttaatct
      181 atataccttt ctggatcaac gaaagtatac tttatatagt cataacgccg atggtaacta
      241 tgcactgtaa cgataatgct gctaaaatat ctttttacga aaaacatcac aaggaaaaac
      301 ttaactatgg tattatctaa acataatttt ttcggtattt aatatttaat taggagggaa
      361 tattttgctt ttttatagta attttatctt aatagtagcg atattattac ttctgaatat
      421 atggattttt gataggagta gaaatgcaag tataggtttt agaactaaac gaagtctgtc
      481 ttctaaaaaa aattgggtat actcacaaac tattttttat ggaggaattg ttttaattag
      541 tttactttct tcaac
//
LOCUS       DASNNU010000277          547 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.286, whole genome shotgun sequence.
ACCESSION   DASNNU010000277 DASNNU010000000
VERSION     DASNNU010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.286"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..>547
                     /locus_tag="U8116_002889"
     CDS             6..>547
                     /locus_tag="U8116_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN8435803.1"
                     /translation="MFIVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTY
                     PTGTIVGDMIGGVFWNYSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALF
                     MSALAELKVFIPELFIIFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLND
                     NGQSYADNFTIQGLLEYASR"
BASE COUNT          176 a           64 c           90 g          217 t
ORIGIN      
        1 ctcgtatgtt catagtatat ataaattgtg caacatattt aacaattaaa gattactata
       61 aagtattcgc tataattgaa aaattattct ggatttcact gccactttgt ttaattgaaa
      121 gattcatggt cacctatcca actggaacaa ttgtaggaga tatgataggt ggagtttttt
      181 ggaattattc aggttgtaat atgccattga atttaatttt gtgtgtgaca gttactgttg
      241 ttacaatcgg atatttcaag ggagaaaaaa atttcatcca ttttatttta tgtgtttgtg
      301 cagcactttt tatgtctgca ttagctgaat tgaaagtttt tattcctgaa ttatttataa
      361 tatttgttgc aatagtattc actttaaaag ttgattttaa agtagtgatc aagatattat
      421 catgcgtaat acttgtgtcg atagtattat ccttttttat aacaatattt attacattga
      481 acgataatgg acaaagctat gctgataatt tcacaattca aggtctttta gaatatgcgt
      541 caagaga
//
LOCUS       DASNNU010000278          528 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.288, whole genome shotgun sequence.
ACCESSION   DASNNU010000278 DASNNU010000000
VERSION     DASNNU010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.288"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(5..90)
                     /locus_tag="U8116_002890"
     tRNA            complement(5..90)
                     /locus_tag="U8116_002890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="U8116_002891"
     tRNA            complement(107..178)
                     /locus_tag="U8116_002891"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="U8116_002892"
     tRNA            complement(190..262)
                     /locus_tag="U8116_002892"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="U8116_002893"
     tRNA            complement(280..361)
                     /locus_tag="U8116_002893"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="U8116_002894"
     tRNA            complement(369..441)
                     /locus_tag="U8116_002894"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="U8116_002895"
     tRNA            complement(445..517)
                     /locus_tag="U8116_002895"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DASNNU010000279          522 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.291, whole genome shotgun sequence.
ACCESSION   DASNNU010000279 DASNNU010000000
VERSION     DASNNU010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.291"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(233..382)
                     /locus_tag="U8116_002896"
     CDS             complement(233..382)
                     /locus_tag="U8116_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435804.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          163 a           82 c           82 g          195 t
ORIGIN      
        1 attatacaga taataacgga atattatcat cgaaacagct aacaaaattg tcataaaaaa
       61 cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa gagagacaat
      121 ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt tacaaacgtc
      181 ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact ccttatctag
      241 attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg ttattttcgt
      301 tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg cggtattgca
      361 tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt agttcttgaa
      421 agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt ttgatgcaat
      481 taaaaagcat ttatcatttt ccttatttat tttttcttaa at
//
LOCUS       DASNNU010000280          517 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.292, whole genome shotgun sequence.
ACCESSION   DASNNU010000280 DASNNU010000000
VERSION     DASNNU010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.292"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            19..>517
                     /locus_tag="U8116_002897"
     CDS             19..>517
                     /locus_tag="U8116_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN8435805.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSED"
BASE COUNT          190 a           48 c          111 g          168 t
ORIGIN      
        1 ataaaggaga aaagtacttt gaagaaaatt tgtttcatta gtggtgtaat ttctcgttct
       61 gggggaactg aaagagtagg gagtattatt gccaatagct tgtctgattt aggttatgat
      121 gtcactattt taagcttttg gaatagtgga gagccttatt ttgggttaga taagacgatt
      181 aaggttgatt atatgttaaa tcctaaaacg gagggaaaat tatatagaac ttatttatat
      241 ccggtttgga aattgcacaa gtatatatgc aaaaatgaat ttgatatagt aattgatatt
      301 gatactttgt tgtctttgta tactacaagg gcaattaaag gaacaaagtg caaattgatt
      361 tcttgggaac attttaatta ctggacaatg gaaatgctga aagaaaagaa gagattcaag
      421 gctaaaaaat tgataaaaaa atatgcaaag aggcttgtgg ttctcacaga agaagatcgt
      481 caaaaacata ttgatgaata tagtttatca gaagatt
//
LOCUS       DASNNU010000281          517 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.293, whole genome shotgun sequence.
ACCESSION   DASNNU010000281 DASNNU010000000
VERSION     DASNNU010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.293"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(36..338)
                     /locus_tag="U8116_002898"
     CDS             complement(36..338)
                     /locus_tag="U8116_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435806.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          176 a           92 c           71 g          178 t
ORIGIN      
        1 atccgttccg ggctaccttt tttaggtagc ctactttaat ctttatacct ttctggatca
       61 acgaaagtat acttcaaata gtcataacgc cgatgatcgc tccgtgcgtc tggcacatca
      121 gtcacgatat caaacaaaaa atacacatct ttcttcattc tggtttttgt tgccggaatt
      181 ttaaagtagt tcttattaga gtaatagaga ttgattaata agctatcttc gattgctaaa
      241 aagaaaactt ctgaatccca caccttataa aaatctttga caaatctatt cgaaggatca
      301 aatttaaacc ataattgtgt ttttccttcc atcaacataa tgttcacctc aaaaagagta
      361 tacgaactaa tgttcttttt gtaaagtagg aatatcattg ataatatgga tggaacatat
      421 atattaaaaa gtgttctttt tgaatgctat tttgaatgcc attttcattc atttaacgaa
      481 aatctgcgaa aataaaaaat gctcaaaccc ttataaa
//
LOCUS       DASNNU010000282          516 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.294, whole genome shotgun sequence.
ACCESSION   DASNNU010000282 DASNNU010000000
VERSION     DASNNU010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.294"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(34..>516)
                     /locus_tag="U8116_002899"
     CDS             complement(34..>516)
                     /locus_tag="U8116_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN8435807.1"
                     /translation="AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMY
                     EQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSK
                     LANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE
                     "
BASE COUNT          166 a           81 c           62 g          207 t
ORIGIN      
        1 cttaataaac tcttctctaa aacaggtact attctattca tctatatttc caactttcaa
       61 aatttcttta tcaattctat ttaatagttt atcagagtaa tccgatttaa cttttcttac
      121 aaataatttt tcactttctt ttatttgatc tatatccctt tcatcaaacc atttgttcat
      181 ggaaggatca ataatatgaa tatttgctaa tttcgaagtt tgtaaagtag tttcttcaat
      241 atttttcaat tcaagtggta aatctggtcc tccaataaca tttttatttt tgaacgagga
      301 attaaaaaaa agtgtatgaa aaaatttttc atctggggca aacataccta ttttaaaatt
      361 caggtagtct tgttgatttt gttcatacat ttgtattaat tctgttaggc attctttcga
      421 caaagcccac cattgagaac catgatacaa atcaaattta tggtgtctaa tttttggggg
      481 aagtttaatc atcctcagac aacgattaat tatagc
//
LOCUS       DASNNU010000283          510 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.295, whole genome shotgun sequence.
ACCESSION   DASNNU010000283 DASNNU010000000
VERSION     DASNNU010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.295"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..508
                     /locus_tag="U8116_002900"
     CDS             <1..508
                     /locus_tag="U8116_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HEN8435808.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELG
                     CANASEAV"
BASE COUNT          155 a           90 c          110 g          155 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      481 atgtgctaac gctagcgagg cagtctaact
//
LOCUS       DASNNU010000284          509 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.296, whole genome shotgun sequence.
ACCESSION   DASNNU010000284 DASNNU010000000
VERSION     DASNNU010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.296"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..132)
                     /locus_tag="U8116_002901"
     CDS             complement(<1..132)
                     /locus_tag="U8116_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN8435809.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DASNNU010000285          478 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.297, whole genome shotgun sequence.
ACCESSION   DASNNU010000285 DASNNU010000000
VERSION     DASNNU010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.297"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          154 a           76 c           65 g          183 t
ORIGIN      
        1 catttcttca tgtttttttc ggatatttgt gctttttaac cattcagcag ctgaaaacat
       61 ataaaaagat atgtatgtat tctttctgaa attaagttca tttctatgcg atgaagcatg
      121 ttttcaaaat attacccttt cttagattaa acttcagagt attcaaataa cggaataagg
      181 agccttatcc cgcattactg aatgagactg tgacataagt aaaaaggtga cttcaaattc
      241 cttcttcgga atttgaaatc accttcttta taattataaa gtatcggttt cacagcattt
      301 ctgaatgatt cctagaatct taaacgaatg attggagtta tcaacatttt ttcactcgtt
      361 tttagcgaaa caaaaaatga catttgtcac agcctcatta aattattgat tctttttctt
      421 tataattgaa ttgtccatat ttaaagtgtc tagtactcta catgaataat taattgct
//
LOCUS       DASNNU010000286          473 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.298, whole genome shotgun sequence.
ACCESSION   DASNNU010000286 DASNNU010000000
VERSION     DASNNU010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.298"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>473
                     /locus_tag="U8116_002902"
     CDS             <1..>473
                     /locus_tag="U8116_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN8435810.1"
                     /translation="YDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDDWDLIIVGKGE
                     LEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMVILEAQSYGLP
                     VISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRMSLNAYNN"
BASE COUNT          171 a           50 c          103 g          149 t
ORIGIN      
        1 attatgataa aaagagattt cttgcagtag ggcgattagt ggaagagaaa aattttgctg
       61 acatgataag tgcttggaaa attgtagaaa aaaaagtaga tgattgggat cttatcattg
      121 taggaaaagg tgaattagaa aaagatcttc gccagcagat aaaagattta aatctgcaaa
      181 gagtaactat agcagggcat tcagaccatg tggaggaata ttataaatct gcatcttgtt
      241 atgtgcttag ttctgtttat gaaggatttc ctatggttat attagaagca cagtcttatg
      301 gattaccagt tatttcttat gattgtaaaa ctggtccgag agatttggta catcataatt
      361 ttaatgggat gttggttgaa gataagaata tagaccaatt agctaagagc atgataatgt
      421 ttactaagaa tactgattta gcaatgagaa tgtcactgaa cgcatataat aat
//
LOCUS       DASNNU010000287          462 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.299, whole genome shotgun sequence.
ACCESSION   DASNNU010000287 DASNNU010000000
VERSION     DASNNU010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.299"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..430)
                     /locus_tag="U8116_002903"
     CDS             complement(<1..430)
                     /locus_tag="U8116_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN8435811.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DASNNU010000288          450 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.300, whole genome shotgun sequence.
ACCESSION   DASNNU010000288 DASNNU010000000
VERSION     DASNNU010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.300"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            263..>450
                     /locus_tag="U8116_002904"
     CDS             263..>450
                     /locus_tag="U8116_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435812.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRT"
BASE COUNT          200 a           58 c           67 g          125 t
ORIGIN      
        1 attgtaaaat gaaaaaaaca ggacgagatt ttctctaatc taaaaataaa aaaagaattt
       61 taaggaaaaa cagagaaata aaatacttaa tataaatgga aagagaaaaa aataatcata
      121 cataggagaa aaaaaacaaa atagaaaaaa gtgattttct aataaattat tcatgtggaa
      181 atgtcatttt tactgtataa ttcatttgat tatccatttt ttattcatca aaattactga
      241 aaaaagaaag gaagaaataa atttgaagaa aacaaattct tcaggaaaac cgaaacgcga
      301 cttcactaaa ttatcgacac cccatacgta tgtcattatt tttggtgtag tcatctttgc
      361 atggatactg acttttgtcg ttccagcagg aaagttcagc acgcaagata ttgaatacaa
      421 agatgcaaat ggcgaaacaa gtactcgtac
//
LOCUS       DASNNU010000289          437 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.301, whole genome shotgun sequence.
ACCESSION   DASNNU010000289 DASNNU010000000
VERSION     DASNNU010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.301"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..437
                     /locus_tag="U8116_002905"
                     /pseudo
     CDS             1..437
                     /locus_tag="U8116_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          179 a           77 c           80 g          101 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattga
//
LOCUS       DASNNU010000290          418 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.302, whole genome shotgun sequence.
ACCESSION   DASNNU010000290 DASNNU010000000
VERSION     DASNNU010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.302"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..274
                     /locus_tag="U8116_002906"
     CDS             <1..274
                     /locus_tag="U8116_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435813.1"
                     /translation="PWHIRYVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKY
                     EIFYYPVTKNTTIHVPTNLRYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          152 a           65 c           79 g          122 t
ORIGIN      
        1 accatggcat attcgctatg ttggtttacc acatagtgcg attatgaaag aaaagaattt
       61 cgttctcgag gaatatatgg attacctaaa agaagaaaaa accatttctg ttagtgtaaa
      121 tggggaaaaa tatgagatct tttattatcc tgttactaaa aataccacca ttcatgtgcc
      181 gactaatctt cgttatgaga tatcaggaaa caatatagac ggtgtaattg tgacagtgtt
      241 tcccggatca acacatacta attcaaggag gtaaggatgg cggaatgaaa ccaacgaaat
      301 taatgaacag cattattgta ctagcacttt tggggtaacg ttagcttttt aatttaaaac
      361 ccacgttaac taggacattg ctatactaat gatacaactt aaacaaaaga attagagg
//
LOCUS       DASNNU010000291          416 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.303, whole genome shotgun sequence.
ACCESSION   DASNNU010000291 DASNNU010000000
VERSION     DASNNU010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.303"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          155 a           65 c           66 g          130 t
ORIGIN      
        1 agaaaacctc cagacttctt gatagctcaa gtatatcttg aggtgcattt gttagtaagg
       61 tactgttcta tggggcgctt actctaggag tgagcatttt tcgaaaaata ctaaaaattt
      121 ttcaaaataa aaaaaggcat acagacaata aactgtctat acgcctcaca gactctattc
      181 tatacatcta attttggctg tcctgcaaaa aatatgtgtt agcctccatc gaaagatcaa
      241 actttggaaa acatcattat aactttgctt ggtaagagct ttgctaaaac atgatatgac
      301 ttgtatacaa aatgtggagt gtattccaat aaataggaag gattacaaaa tttgaaacca
      361 aataagtaaa aaatactgtc actttgaatt aatttgaaat agtaaattct cattaa
//
LOCUS       DASNNU010000292          411 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.305, whole genome shotgun sequence.
ACCESSION   DASNNU010000292 DASNNU010000000
VERSION     DASNNU010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.305"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>411
                     /locus_tag="U8116_002907"
     rRNA            <1..>411
                     /locus_tag="U8116_002907"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DASNNU010000293          401 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.306, whole genome shotgun sequence.
ACCESSION   DASNNU010000293 DASNNU010000000
VERSION     DASNNU010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.306"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>401)
                     /locus_tag="U8116_002908"
     CDS             complement(<1..>401)
                     /locus_tag="U8116_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEN8435814.1"
                     /translation="FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHS
                     SDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKR
                     AHGNMHNGFLQVLVSSGIIAFICIYAFYVLN"
BASE COUNT          134 a           75 c           52 g          140 t
ORIGIN      
        1 tatttaacac atagaaagca tagatacata taaaagcaat tataccactt gatacaagta
       61 cttgaagaaa tccattatgc atgtttccat gtgctctttt tagttcattc acatctgtag
      121 tatcaagatc tacttttttc aaaaattttg catcgctatt cccatagaaa tcggtctctc
      181 cataaccaaa taacaaagtt ggtcttataa ctttaagcgc agctttccaa atcgtaaaac
      241 gaccagctga aatttcggag ttatcactag aatgctgtaa tattacttct ttttctggaa
      301 gtggagtttt atctgattca tttgactcta taacttctac ttttctatca atcgatacag
      361 gaattgttgc taaaaaatcc ttagcataat tagtaccaaa a
//
LOCUS       DASNNU010000294          397 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.307, whole genome shotgun sequence.
ACCESSION   DASNNU010000294 DASNNU010000000
VERSION     DASNNU010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.307"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            118..336
                     /locus_tag="U8116_002909"
                     /pseudo
     CDS             118..336
                     /locus_tag="U8116_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          134 a           57 c           70 g          136 t
ORIGIN      
        1 tcagttttga ggaaatgaaa ttctagatat tttttgctac atagagttac tttcaatatt
       61 ttttaacgta attaggcaga agtcactcat cctctatagg tttgtgatga aattgccatt
      121 gatccattta agtaagagca aaaaactgga ggtttatcag gtaattggtc taatggattt
      181 aagtatgcgg tcaccgtgta tagagttgct tatgagattc aagaaaataa agttattcca
      241 gtattgctat atggaacaca taacaatttt tatgaattag ccgagaacac tcgcaacttt
      301 attcgtgtag atgaatcggc ttcggttgag ttataacttt agctatatca atcaaccgac
      361 cgacatatgt taaatctata tataataaaa ataccta
//
LOCUS       DASNNU010000295          379 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.309, whole genome shotgun sequence.
ACCESSION   DASNNU010000295 DASNNU010000000
VERSION     DASNNU010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.309"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          129 a           70 c           79 g          101 t
ORIGIN      
        1 acttacgacg atacagaaga aatccaaaat gaacttgacc gtaaacgaat gccatatcaa
       61 cgatttgaaa ggaaaaaagg taaataacca tttataccga aaagtgagga tgtgtgaaat
      121 aagaacaaaa aaccttgaaa ttaagggaat ggttcttatt tcgcaccaat cgctccaacc
      181 gcccccgccg cttaggtggt gcgaaataag cgggcaaagc ccgataacct aagagaaaga
      241 aggagatcca tatgaataaa atcattcctc tattgttcgt actgtttgtt ggcgagatac
      301 ttatgcgtca tgctgcagct gtacatttaa acttcgtagt attcttagga atactggtat
      361 tttgtttgat cttttggcg
//
LOCUS       DASNNU010000296          379 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.310, whole genome shotgun sequence.
ACCESSION   DASNNU010000296 DASNNU010000000
VERSION     DASNNU010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.310"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="U8116_002910"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="U8116_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEN8435815.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          122 a           57 c           63 g          137 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atggaacaaa ataaaaaggc
      361 aatacgcctt agttgtcgg
//
LOCUS       DASNNU010000297          374 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.311, whole genome shotgun sequence.
ACCESSION   DASNNU010000297 DASNNU010000000
VERSION     DASNNU010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.311"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..132
                     /locus_tag="U8116_002911"
     CDS             <1..132
                     /locus_tag="U8116_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN8435816.1"
                     /translation="MPESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            133..>374
                     /locus_tag="U8116_002912"
     CDS             133..>374
                     /locus_tag="U8116_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN8435817.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELA"
BASE COUNT          141 a           47 c           77 g          109 t
ORIGIN      
        1 atgcctgaat ctgctaatga tatgtatgag aaggagttga aatatttctt tgaaaacgta
       61 gtaacaggca aagaaaactg gaatgattta tatcatgcaa acgaagtatt gaaaatagct
      121 gaggagaaat aaatggaaat tttatttact atttgtggta gagctgggtc taaggggttt
      181 aaaaataaaa atttgaaata tttggcagga aagcccctag tccatttcgc tttagcaatg
      241 atagatatct atcaacaaaa acatcctgaa cataattgca cgacttgttt gaatacggac
      301 agtaatgagt tagtagaatt gacgactaag ttaaatccgt ctgtgaaaat tgtagaacgt
      361 aaacctgaat tagc
//
LOCUS       DASNNU010000298          370 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.312, whole genome shotgun sequence.
ACCESSION   DASNNU010000298 DASNNU010000000
VERSION     DASNNU010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.312"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>370
                     /locus_tag="U8116_002913"
     CDS             82..>370
                     /locus_tag="U8116_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN8435818.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          132 a           59 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa
//
LOCUS       DASNNU010000299          368 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.313, whole genome shotgun sequence.
ACCESSION   DASNNU010000299 DASNNU010000000
VERSION     DASNNU010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.313"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..257
                     /locus_tag="U8116_002914"
     CDS             <1..257
                     /locus_tag="U8116_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435819.1"
                     /translation="SKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKKW
                     LVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          160 a           42 c           54 g          112 t
ORIGIN      
        1 tgtcaaaatt agaaaacaaa gaagaaaaag ttgtaaacaa aattgtttct gttgtcaata
       61 aactggataa agagttagac gaacttgata cgttatctga aaatccagaa aaaaaacata
      121 acctaaaaaa atggttagtt gaacgaaagg ctattcatga aattaaaaaa gttcttcatg
      181 aagctgataa atatgaaaaa tatgatgaaa aagaacttga caaagaattt aaagaaatca
      241 atgacttatt actataattg atttatacga ctcatttttg agtcgtcaga ctgcaaacaa
      301 cactctaaat ttaggagttt gtctacagtc tgaataaatt ttaaatttat tgtccataat
      361 gttattat
//
LOCUS       DASNNU010000300          365 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.314, whole genome shotgun sequence.
ACCESSION   DASNNU010000300 DASNNU010000000
VERSION     DASNNU010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.314"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>365
                     /locus_tag="U8116_002915"
     CDS             <1..>365
                     /locus_tag="U8116_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435820.1"
                     /translation="YTDAKWPEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKV
                     TTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEV
                     DVTVTDNRSDADKYEPTVEG"
BASE COUNT          150 a           54 c           83 g           78 t
ORIGIN      
        1 tatacagatg cgaaatggcc agaggaagca actcaaccaa ttacagcaga tgatacagaa
       61 tttgtatcaa gtgcaacaaa actggatgat aaatctgacg ctgacaaata taatcctgaa
      121 ggtcaaaaag tgactacaga attgaataag gaaccagacg catttgaggg aattaagaac
      181 aaagaagatt taccaaaaga tactaagtat acttggaaag aaaaagtaga tgttagcgca
      241 gctggaaata aaaaagggac tgttgtagtg acatatccag atggatcatc tgatgaagtt
      301 gaagtagatg ttacagtgac agacaatcgt tctgacgccg ataaatatga gccaacagta
      361 gaagg
//
LOCUS       DASNNU010000301          362 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.315, whole genome shotgun sequence.
ACCESSION   DASNNU010000301 DASNNU010000000
VERSION     DASNNU010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.315"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>362)
                     /locus_tag="U8116_002916"
     rRNA            complement(<1..>362)
                     /locus_tag="U8116_002916"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DASNNU010000302          358 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.denovo.316, whole genome shotgun sequence.
ACCESSION   DASNNU010000302 DASNNU010000000
VERSION     DASNNU010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.denovo.316"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..265)
                     /locus_tag="U8116_002917"
     CDS             complement(<1..265)
                     /locus_tag="U8116_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN8435821.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVP"
BASE COUNT          119 a           67 c           42 g          130 t
ORIGIN      
        1 ctggaacgtg tttcattgcg gcagcatgaa aaataatatc cacatcacga catgcataat
       61 caatcctttg agagtctctg atatcaccaa ttaaaaatct caaattagtg tgatttgaga
      121 gctcttgttg catataaaat tgtttttctt cacctctact ataaattctg atagcttttg
      181 gttcatattt taaaagctga cgaacaattt cacttccaat ggaaccagtt ccgccagtta
      241 ctaatattgt ttttccttta aacacttata tctcctccta tgaattatag tcatctataa
      301 ttcgattctt aatctttaaa aaatatataa ctaaactaat tatcaataat gatgtcaa
//
LOCUS       DASNNU010000303         7695 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.guided.111, whole genome shotgun sequence.
ACCESSION   DASNNU010000303 DASNNU010000000
VERSION     DASNNU010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.guided.111"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..608
                     /locus_tag="U8116_002918"
     CDS             <1..608
                     /locus_tag="U8116_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN8435822.1"
                     /translation="DFILYLKLCNRTKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAM
                     TDKAVKKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEV
                     ELKRDMVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFK
                     KLIREISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            738..1385
                     /locus_tag="U8116_002919"
     CDS             738..1385
                     /locus_tag="U8116_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HEN8435823.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(2078..3016)
                     /locus_tag="U8116_002920"
                     /pseudo
     CDS             complement(2078..3016)
                     /locus_tag="U8116_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(3016..3633)
                     /locus_tag="U8116_002921"
     CDS             complement(3016..3633)
                     /locus_tag="U8116_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN8435824.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(3647..3817)
                     /locus_tag="U8116_002922"
     CDS             complement(3647..3817)
                     /locus_tag="U8116_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435825.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(4165..5655)
                     /locus_tag="U8116_002923"
     CDS             complement(4165..5655)
                     /locus_tag="U8116_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN8435826.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(5698..5997)
                     /locus_tag="U8116_002924"
     CDS             complement(5698..5997)
                     /locus_tag="U8116_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435827.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(6363..6647)
                     /locus_tag="U8116_002925"
     CDS             complement(6363..6647)
                     /locus_tag="U8116_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8435828.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(7166..7651)
                     /gene="vanZ"
                     /locus_tag="U8116_002926"
     CDS             complement(7166..7651)
                     /gene="vanZ"
                     /locus_tag="U8116_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEN8435829.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT         2415 a         1285 c         1184 g         2811 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccaa acaaaatatt atcgactaca
       61 tggaagatga cggttttaca agattagact tagcttttga ttttgaagat gatttgagcg
      121 attactatgc aatgactgat aaagcagtta agaaaactgt tttttatggt cgtaatggca
      181 agccagaaac aaaatatttt ggtgtccgtg atagtgatag atttattaga atttataata
      241 aaaaacaaga acgtaaagat aacgcagatg ttgaagttgt gtctgaacat ttatggcgtg
      301 tagaagttga attaaaaaga gatatggttg attactggaa tgattgtttt aatgatttac
      361 acatcttgaa acctgcgtgg gctactttag aaaaaattaa tgagcaagct atggtttata
      421 ctttgttgca tgaagaaagt atgtggggaa agctaagtaa gaatactaag actaaattta
      481 aaaaattgat tagagaaata tctccaattg atttaacgga attaatgaaa tcgactttaa
      541 aagcgaacga aaaacaattg caaaagcaga ttgatttttg gcaacgtgaa tttaggtttt
      601 ggaagtaaaa taagttttat ttgataaaaa ttgctaattc agtataatta atatttacga
      661 ggtgacataa cgtatgaaaa aatcagagga ttattcctcc taaatataaa aatttaaaat
      721 ttaggaggaa gttatatatg acttttaata ttattgaatt agaaaattgg gatagaaaag
      781 aatattttga acactatttt aatcagcaaa ctacttatag cattactaaa gaaattgata
      841 ttactttgtt taaagatatg ataaaaaaga aaggatatga aatttatccc tctttaattt
      901 atgcaattat ggaagttgta aataaaaata aagtgtttag aacaggaatt aatagtgaga
      961 ataaattagg ttattgggat aagttaaatc ctttgtatac agtttttaat aagcaaactg
     1021 aaaaatttac taacatttgg actgaatctg ataaaaactt catttctttt tataataatt
     1081 ataaaaatga cttgcttgaa tataaagata aagaagaaat gtttcctaaa aaaccgatac
     1141 ctgaaaacac cataccgatt tcaatgattc cttggattga ttttagttca tttaatttaa
     1201 atattggtaa caatagcagc tttttattgc ctattattac gataggtaaa ttttatagtg
     1261 agaataataa aatttatata ccagttgctc tgcaacttca tcattctgta tgtgatggtt
     1321 accatgcttc actatttatg aatgaatttc aagatataat tcatagggta gatgattgga
     1381 tttagttttt agattttgaa agtgaattta attttataca cgtaagtgat cataaaattt
     1441 atgaacgtat aacaaccaca ttttttggtt gcttgtggtt ttgattttga atttggtttt
     1501 gaacttatgg actgatttat tcagtccatt ttttgtgctt gcacaaaaac tagcctcgca
     1561 gagcacacgc attaatgact tatgaaacgt agtaaataag tctagtgtgt tatactttac
     1621 ttggaagatg caccgaataa aaaatattga agaacaacta gcaaaagatt ttaaagagtt
     1681 attttatttt aagtctttat aacatgagtg aagcgaattt ttaaatttcg atagaaattt
     1741 ttacatcaaa aagccccctg tcaaaattga cgaagggggt tttttggcgc acgcttttcg
     1801 ttagaaatat acaagattga aaatcgtgta taagtgcgcc ctttgttttg aacttagcac
     1861 gttacatcaa ttttttaaaa tgatgtataa gtgcgccctt ttaaattttg agtgattata
     1921 ttttttgagt tagaaaaagg gattgggaaa atttcccaaa ataatttaaa aaataagcaa
     1981 aaattttcga tagagaatgt gctatttttt gtcaaaggtg tataccttga ctgtgcttgc
     2041 tgttacatta agtttatttt taagttatta aaaaagaaat agcttttaaa gtttggctcg
     2101 ctgtcgcttt ataaagctga ttgacttttg attgcaaact acttaaagaa aacaaactcg
     2161 gactattcgt tttcttctct ttggtttgaa catcagcaat tatcccctct tgattgccta
     2221 ttttagcttg tttagaagaa acaaaagcta aaagctcctc ttgggtttta aaacgctgtg
     2281 tggggcttag aacgccctta aacgaccctt ggtttacttt tatactagct tccacctcga
     2341 aaaaaggttc ttttttaaaa ttctctatgg cttcctggcg ctgaaaaata aggtataagg
     2401 tgggcgtttg aacacgtcct agtgaaaatg taccttgtac gcccttctgt tgtaaattta
     2461 acgtatacaa agggcttgcg ttcatgccga tcaaccaatc ggcaatttgg cgtgtttgcg
     2521 cttcttgata aaagggatag taattcattc caggttgcaa attttgaaaa ccgcttcgga
     2581 ttacatcttt ttctaagcta ttgatccata gtcttttaaa tgttttatct tttgaaaagg
     2641 catttgcttt atggataatc gaccaggcga tattttcacc ttctctgtcg ctatctgttg
     2701 caacaataat tgtatttgcc tttttgagaa gttctgcaac aattttaaac tgctttccct
     2761 tatcttttgc aacttcaaaa tcgtatcgat caggaaaaat cggcaaagat tcaagtttcc
     2821 aattttgcca cttttcgtca taatgacctg gttctgctaa ttccactaaa tgcccaaaac
     2881 caaaggtgat aaacgtttca tctgtaaata gtgggtcttt gatctcaaaa taaccgtctt
     2941 ttttggtgct ttgttttaaa gcacttgcgt aggctaatgc ctggcttggt ttttcagcta
     3001 aaataaccgt actcattaac tatccctctt ttcattgttt tttctttgat cgactgtcac
     3061 gttatatctt gctcgatacc ttctaaacgt tcggcgattg attccagttt gttcttcaac
     3121 ttctttatcg gataaaccat tcaaaaacaa atcgaaagca tgctttaaac gtggatcatt
     3181 ttctttaaat ttatgctgac gacctttgaa tttgcctttt ttcttagcaa tttcgattcc
     3241 ttgtgcctga cgttccttaa ttttttttcg ttctgattct gcttgatact tgtacaattc
     3301 aatgacaagg ctattaatca aacgccttaa attttcatct tcaataccat tcattgaggg
     3361 taaatttaag acttccaggg ttgccccctt aatttgaatt tgattcatca attctgttaa
     3421 ttctttatta tttcgtccta atcgatctaa ttcagtaaca ataacaatat ccccttcacg
     3481 aatatagtta agcatagctt gtaattgtgg gcgttcgacc gattgaccgc ttaatttgtc
     3541 tgaaaagacc ttagaaacgc cctgtaacgc ttgtaattgc cgatctaagt tctgttcttt
     3601 gctactgaca cgtgcataac caattttagc cattttcaac caacctctaa aattctctcg
     3661 gttgcaataa ccaatcagca atatctactt tttcaatttc aaattgctta tcagaaattg
     3721 tcttttcgta agcgataaaa tcttgcgcat attgttgctc attaaaaata gccaccactt
     3781 cgtcattttc taaaactcga taaataaatt ttttcatttt actcctccta ttatgcccaa
     3841 cttaaatgac ctattcacca agtcaattat actgctaaaa tcatattagg acaaataggt
     3901 atactctaat gacctataaa agatagaaaa ttaaaaaatc aagtgttcgc ttcgctctca
     3961 ctgcccctcg acgttttagt agcctttccc tcacttcgtt cagtccaagc caactaaaag
     4021 ttttcgggct actctctcct tctcccccta ataattaatt aaaatcttac tctgtatatt
     4081 tctgctaatc attcgctaaa cagcaaagaa aaaacaaaca cgtatcatag atataaatgt
     4141 aatggcatag tgcgggtttt attttcagcc tgtatcatag ctaaacaaat cgagttgtgt
     4201 gtccgtttta gggcgttctg ctagcttgtt taaagtctct tgaatgaatg tatgctctaa
     4261 gtcaaaagaa tttgtcagcg cctttatata gctttctttt tcttcttttt ttactttaat
     4321 gatcgatagc aacaatgatt taacactagc aagttgaatg ccaccatttc ttcctggttt
     4381 aatcttaaag aaaatttcct gattcgcctt cagtaccttc agcaatttat ctaatgtccg
     4441 ttcaggaatg cctagcactt ctctaatctc ttttttggtc gtcactaaat aaggcttgta
     4501 tacatcgctt ttttcgctaa tataagccat taaatcttct ttccattctg acaaatgaac
     4561 acgttgacgt tcgcttcttt ttttcttgaa tttaaaccac ccttgacgga caaataaatc
     4621 tttactggtt aaatcacttg atacccaagc tttgcaaaga atggtaatgt attccctatt
     4681 agccccttga tagttttctg aataggcact tctaacaatt ttgattactt ctttttcttc
     4741 taagggttga tctaatcgat tattaaactc aaacatatta tattcgcacg tttcgattga
     4801 atagcctgaa ctaaagtagg ctaaagagag ggtaaacatg acgttattac gccctattaa
     4861 acccttttct cctgaaaatt tcgtttcgtg caataagaga ttaaaccagg gttcatctac
     4921 ttgttttttg ccttctgtac cgcttaaaac cgttagactt gaacgagtaa agcccttatt
     4981 atctgtttgt ttgaaagacc aatcttgcca ttctttgaaa gaataacggt aattaggatc
     5041 aaaaaattct acattgtccg ttcttggtat gcgagcaata ccaaaatgat tacacgttag
     5101 atcaactggc aaagactttc caaaatattc tcggatattt tgcgaaatta ttttggctgc
     5161 tttgacagat ttaaattctg attttgaagt cacatagact ggcgtttcta aaacaaaata
     5221 tgcttgataa cctttatcag atttgataat catagtaggc ataaaaccta aatcaatagc
     5281 ggttgttaaa atatcgcttg ctgaaatagt ttcttttgcc gtgtgaatat caaaatcaat
     5341 aaagaaggta ttgatttgtc ttaaattgtt ttcagaatgt cctttcgtgt atgaacggtt
     5401 ttcgtctgca tacgttccat aacgataaac gttgggtgtc caatgtgtaa atgtatcttg
     5461 attttcttga atcgcttcct cggaagtcag aacaacacca cgaccgccaa tcatgcttga
     5521 ttttgagcga tacgcaaaaa tagccccttt gcttttacct ggcttggtag tgattgagcg
     5581 aattttacta tttttaaatt tgtactttaa caagccgtca tgaagcacag tttctacaac
     5641 aaaagggata ttcattcagc tgttctcctt tcctataaat cctataaaat aggttgttta
     5701 attaacttgg tttgcttttt cattcaactg tttcaatatt gcatgttttg aaaaagattt
     5761 ttttccttta taagtcaatt tttttccact aatcgaataa attattttgt tattttctat
     5821 taacttatat atataatctt ccccctccga agaaaaatac ttatctgatt ttgtttctaa
     5881 gtagatattt ctcttttcta actctttctt aaacgtttct agtgtataga tatttgctaa
     5941 ttttcttatc tccaataaac tattttttat ataagtttta cattcatcat gattcataca
     6001 aactccacct tctataaatg aatacaaaaa aagcaatcaa acgatttccg attgattgct
     6061 taacaattct taaattcagt agcttagata cttgaaaact ctctgatttc cctatataat
     6121 gatagtacgg ttatataccg tcttcaaaca aagttaatta aataacttct tacgagggaa
     6181 gagttcatct gactaactga taagcgttgg tttggcaatc ttatcgggct atgcatttat
     6241 aaaatgtcgt caaacatttt ataaatgtgt catggctctt ttttcgtttc tattcagttc
     6301 gttgtttcgt tatatctagt ataccgcttt taaaaaaaat aagcaacgat ttcgtgcatt
     6361 attcacacga agtcattgct tttttcttct tccatttcta aatccaatgt tacttgttct
     6421 gattctgttt ctggttctgg ttctgttggc tcatttggga ttaaatccac tactagcgtt
     6481 gagttagtta actttgcaat ttgttctagt gtttttatgg ttggatctga ttttcctgat
     6541 tctattcgtg aataatttga tctactcatt tctaattctt gggagaattt ttcttttgat
     6601 ttggtacctc tcatcttttt taagctttct ctaaatgcta gttccaagtt tctcccccct
     6661 ctttttgtgt aataaaattc acgctttatt taaattatag cttttaatat cttaataaac
     6721 aagtgattaa tcacttgtga tttgtgatta ttccaaaagt aggtaatttc accaaccttt
     6781 ttcaaaagtt tttatcgacg attattggac tggatcgtgt ttcctaaagc gattctaagc
     6841 gcatgtttaa ttttaaagtg ggtaattctc tttaaaacgt cctacaaaga ttcaaaaaaa
     6901 gcatggctgc tagtgggggt accgtcagga aaatgcggat ttacaacgtt aaggaaattt
     6961 attaaagaga ccaattcctc agcactaaaa ttgataattg ggctttcttt gttactatag
     7021 tgttaggtaa gctttttact ttatcaccgg ttttcccctc acttcacacc tacgggcgag
     7081 tttccccaca tcccgttttc catctaatcc aattcattca atatctttat gtgtatgaaa
     7141 gatcagattg cttgatttag acatcttaca cgtaatttat tcttaaatgg gtacggtaaa
     7201 cgagcaataa tacgagcaaa agtataccta caaaaataat aactctgtct aatttttttt
     7261 gattcatatg cttattgctt aaaccatata atttcagtcc aagaaagcct ccaacagtat
     7321 ttgtaattac atctgttatg tctgtcgctc caatagcgaa gataaattga attatttcaa
     7381 aagtaagact taaaaccagt acaaaagcaa acttaggtaa aaatccgatt tctttaaaat
     7441 tgacattcaa aagcaagcca aatggaataa agattataac attatctatc atctctctga
     7501 aattcccagt agcagtaaat ggagtcaagt taagacttct ttgatgataa ttaaatactg
     7561 ataaaatatt gtattgtaat ttgaataaca ctaaccagat tagtgtcact aaatataaag
     7621 ctagcaatcc tctagataat atttttccca atataatttc ctctaattct tttgtttaag
     7681 ttgtatcatt agtat
//
LOCUS       DASNNU010000304         6119 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.guided.121, whole genome shotgun sequence.
ACCESSION   DASNNU010000304 DASNNU010000000
VERSION     DASNNU010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.guided.121"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1025)
                     /locus_tag="U8116_002927"
     CDS             complement(<1..1025)
                     /locus_tag="U8116_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN8435830.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHIT"
     gene            complement(1104..1310)
                     /locus_tag="U8116_002928"
     CDS             complement(1104..1310)
                     /locus_tag="U8116_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEN8435831.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(1303..2445)
                     /locus_tag="U8116_002929"
     CDS             complement(1303..2445)
                     /locus_tag="U8116_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN8435832.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            2957..3592
                     /locus_tag="U8116_002930"
     CDS             2957..3592
                     /locus_tag="U8116_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN8435833.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            3746..3823
                     /locus_tag="U8116_002931"
                     /pseudo
     CDS             3746..3823
                     /locus_tag="U8116_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            4068..4184
                     /locus_tag="U8116_002932"
     CDS             4068..4184
                     /locus_tag="U8116_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEN8435834.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            4200..6119
                     /gene="tet(M)"
                     /locus_tag="U8116_002933"
     CDS             4200..6119
                     /gene="tet(M)"
                     /locus_tag="U8116_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEN8435835.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT         1832 a         1128 c         1211 g         1948 t
ORIGIN      
        1 gtgatatgtg gcaatggatt gtccttgtgg tgcttgttgt atttctttac catacggata
       61 aataaggcgt tataatcaat cgcaactttg ggcttgccct tatgattgac aaataggaag
      121 ttgccccgtc cgtctatcac aaatggttct gcctttggac gttttttcat aacctgttga
      181 aatgcctgta tcgtttcttt gcttaatggt acttgcctta ttccgctttt tgttttaggc
      241 gtttcaatat aatagccctg ttccttgctc tttagtaact ggtggtcgat aatcacaact
      301 tcattcttga aatcaatgtc ggctactgtc agtccgcaca gttccgaaat acgaagtcct
      361 gtctttaaca gtatcagcac atcatcataa tgcttgtgat acacattgtc cgtcttgatg
      421 aatgagagta aggcttgttc ctgttcctct gtcaatgcga ctttcttttt ggtatcattt
      481 tctaggactt cactcaactt gaaatcaaag ggatttttcc tcacataatc atcttgaatg
      541 gctatgtaga atgacgcttt taacgagcgt ttatggttgt taatggtatt gtaggaaaag
      601 cctttgtctt tcatgcgtaa cgcccattcc ttagcgtcag agggtttgat tgtatcaatg
      661 ctcctagcac ctaacttgtc ctctttcaat aaccgcatga gttgttcccg ttgtttctgt
      721 gtgcttttct tcacatttgc cctctgtgcg ttctgtttgg cataaagttg gcaaagcgtc
      781 attttcttgc cagtgctgtc gataccgtcc tcaatatccc gtcttatctg ctgttccagt
      841 tctcgaagtg agggtttttc ccgttttccc tttggtgtcg ggtctgtggg tgtcaatctc
      901 caagcataca catattttgt gtttccaaat gcgtctacat atttatataa gtattttctg
      961 tctgttcgtt ggctctctcc agtatgcagg atacgacctt tgttatcccg tcttttttct
     1021 ttcatggtgt ctgctccttt ccttgttgga aagagccttg atatgacttg tgttcatcat
     1081 aacacataca aggctcattt gcattagata gcgtccaatt tatcaatgac ctgttcaaac
     1141 tgtcggcgtt taatctgtat gcggttgcca ttcataatga gccagccagc gtccttgttt
     1201 tcctctgcca atcgtcttaa cttgttttct ccgatacgga aatactttga cgcttcttca
     1261 atggtaaggg tgtatttttc ccatacggga atatcctgtg cattactcat aaaacacccc
     1321 ctcactttcg attacgtctg ctatgtccgt acacatctgt tccacaagca tttcctgctt
     1381 ttcagagagg tgggtacatt catcaatcat tttcatgtag tttgagcctg tcagcctgtc
     1441 gatttcctct atcatcacga ttgacggcat gacctgtctt tccagccatt gcagagtgcg
     1501 ttccatattg acaggtacgg ggttcatttc aaaaggtaaa ctgtcatggg aaatgaatat
     1561 ctcccacagc ggatagtcgg gaaattgcac aaaatcagta ataaggtaaa acaccagccc
     1621 atgagggtta tatgtcaata acaattcctc aactgcctgt actgcttttg taccacgcag
     1681 acgaatttca aaacgattga taatatccgt atgtttcttt ttggttgcct gttccttttg
     1741 tttttcatac aggcagaagt attttgtgct tgcctttgaa ccgatataaa gggtgcttgc
     1801 taaattacgg ttttttccgc ttagttttcc accctgcaca ttttcataat ttttacagcg
     1861 acaatccgca ccgccatttc tgtatttttc agacaaaacg ggaatatcca acagaccaca
     1921 catatcattg attgccaagt caaaacgtcg gataacaccg tcacagtcaa gacagcggtt
     1981 taaaaatgaa taccaatctc tgttctgtgc ctgcaagaca tattccatgt tgcgtgagcc
     2041 tgcccctttt aattccaaga atacgcccat gtgttcgtca tcagaagcca tgaccttgat
     2101 ttctccgtag gcgtaatgct ctttatagcc ataataccca tagtcataat ggataaaata
     2161 atctgctttc atgccaagca cctttcggat aatttccaat gcgtcagtcg ttggataacg
     2221 gacagacaga taatcaacga gcagataaaa aggcttatcg cagatataat aattcagaca
     2281 acgcaggata atattttgtg tttccttgct ggcgttggct tttccgttct caaagcggtt
     2341 gatactatat cttgatacat gggtaagact tgcaagttct ccttgtgaaa gaccgcttgc
     2401 aagacgtttg ttcttcattt caagtgcaaa ttctaaattg ttcattgtat tcctccaatc
     2461 ccacaatagc cttgaaatac ctgtaaacct ctattcaaaa agtgtgatat gcactatctt
     2521 cttaaaactt ctcattacta ctcacaactt tgggattgta tggtttttga cttgtctttt
     2581 gtgaaatagt gcccccctgt tagataacgg gggcacagac aaggctcgca ggctcgccag
     2641 ccgacaaagc atttcttccc cttgacacaa ggtgtcatgg cggtaagcct gagaggcttc
     2701 cgccaccgtg tcagaaatac tttgtcagca agttctgttt tgccttttct aacaagttcc
     2761 atttcaaccg atattcatat ctacggattt aatcagatag gtatttcttt ttgaatgttt
     2821 ttatatttca ttctattgtg cttattcgat tacaagaaca tagtaattct tatatgctta
     2881 tttgtcaata tctttttttg cctttacaaa tcatattaag catgatagac ttataatagc
     2941 gaagatataa cgagatatgg agggttatgc aatgaaaata agccatttag gaaataatat
     3001 acagaccata agaaaattta ggggaatgaa acaacaggaa cttgcggaca aaatcggtat
     3061 caatatgcag agcctttcca agattgaaag agggttgaac tatcctgcct atgaaacgct
     3121 ggaaaagata atggaagtgc tggacgttac gcccaatgaa ttgttgtcgg gagaatggaa
     3181 gtatgtcaat caatctgaaa aggaagtctg tcagttttta agagccgaag aacgtctaaa
     3241 tgcagaactg aaacatggac actatgataa cttctttgac agcgaggaag aatggctgga
     3301 atatgagtta gaaaagttac gggaatatat tacagactat atcaacggga aaagcattga
     3361 agcgtccgac ctttacccaa tcaaagaatt tatccagcat ttgaagtttc aaaaattatt
     3421 agaccgctat gatgatttat acagtatgga tatgtttggg gaaagcatag agggacataa
     3481 gtataagaca ccttatcaag tcgtgaaaat gataaacccg aactcaaaag aagatatgga
     3541 actgttacga gaagtattaa ggaataacaa ttttgatgat gaggacgagt aacttttttc
     3601 ctcttgcgag aagatacagc aaacaaaaag cccgatcaag agtgcaaagc accaccaatg
     3661 gtggcaaagc tcttgacagg gctttttctt ttctgttgtg ggcaatcaag aggaagaaag
     3721 ggataaaatc tgatttgtgg taaatcaggc aaaggcaacg caggtatctc aatttgattt
     3781 ggtacttgaa aagaacggga gtaattggaa gattatagaa taacaaatat tggtacatta
     3841 ttacagctat tttgtaatca cgtactctct ttgataaaaa attggagatt cctttacaaa
     3901 tatgctctta cgtgctatta tttaagtatc tatttaaaag gagttaataa atatgcggca
     3961 aggtattctt aaataaactg tcaatttgat agtgggaaca aataattgga tgtccttttt
     4021 taggagggct tagttttttg tacccagttt aagaatacct ttatcatgtg attctaaagt
     4081 atccggagaa tatctgtatg ctttgtatgc ctatggttat gcataaaaat cccagtgata
     4141 aaagtattta tcactgggat ttttatgccc ttttgggttt ttgaatggag gaaaatcaca
     4201 tgaaaattat taatattgga gttttagctc atgttgatgc aggaaaaact accttaacag
     4261 aaagcttatt atataacagt ggagcgatta cagaattagg aagcgtggac aaaggtacaa
     4321 cgaggacgga taatacgctt ttagaacgtc agagaggaat tacaattcag acaggaataa
     4381 cctcttttca gtgggaaaat acgaaggtga acatcataga cacgccagga catatggatt
     4441 tcttagcaga agtatatcgt tcattatcag ttttagatgg ggcaattcta ctgatttctg
     4501 caaaagatgg cgtacaagca caaactcgta tattatttca tgcacttagg aaaatgggga
     4561 ttcccacaat cttttttatc aataagattg accaaaatgg aattgattta tcaacggttt
     4621 atcaggatat taaagagaaa ctttctgccg aaattgtaat caaacagaag gtagaactgt
     4681 atcctaatat gtgtgtgacg aactttaccg aatctgaaca atgggatacg gtaatagagg
     4741 gaaacgatga ccttttagag aaatatatgt ccggtaaatc attagaagca ttggaactcg
     4801 aacaagagga aagcataaga tttcagaatt gttctctgtt ccctctttat catggaagtg
     4861 caaaaagtaa tatagggatt gataacctta tagaagttat tactaataaa ttttattcat
     4921 caacacatcg aggtccgtct gaactttgcg gaaatgtttt caaaattgaa tatacaaaaa
     4981 aaagacaacg tcttgcatat atacgccttt atagtggagt actacattta cgagattcgg
     5041 ttagagtatc agaaaaagaa aaaataaaag ttacagaaat gtatacttca ataaatggtg
     5101 aattatgtaa gattgataga gcttattctg gagaaattgt tattttgcaa aatgagtttt
     5161 tgaagttaaa tagtgttctt ggagatacaa aactattgcc acagagaaaa aagattgaaa
     5221 atccgcaccc tctactacaa acaactgttg aaccgagtaa acctgaacag agagaaatgt
     5281 tgcttgatgc ccttttggaa atctcagata gtgatccgct tctacgatat tacgtggatt
     5341 ctacgacaca tgaaattata ctttctttct tagggaaagt acaaatggaa gtgattagtg
     5401 cactgttgca agaaaagtat catgtggaga tagaactaaa agagcctaca gtcatttata
     5461 tggagagacc gttaaaaaat gcagaatata ccattcacat cgaagtgccg ccaaatcctt
     5521 tctgggcttc cattggttta tctgtatcac cgcttccgtt gggaagtgga atgcagtatg
     5581 agagctcggt ttctcttgga tacttaaatc aatcgtttca aaatgcagtt atggagggga
     5641 tacgctatgg ctgtgaacaa ggattgtatg gttggaatgt gacggactgt aaaatctgtt
     5701 ttaagtatgg cttatactat agccctgtta gtaccccagc agattttcgg atgcttgctc
     5761 ctattgtatt ggaacaagtc ttaaaaaaag ctggaacaga attgttagag ccatatctta
     5821 gttttaaaat ttatgcgcca caggaatatc tttcacgagc atacaacgat gctcctaaat
     5881 attgtgcgaa catcgtagac actcaattga aaaataatga ggtcattctt agtggagaaa
     5941 tccctgctcg gtgtattcaa gaatatcgta gtgatttaac tttctttaca aatggacgta
     6001 gtgtttgttt aacagagtta aaagggtacc atgttactac cggtgaacct gtttgccagc
     6061 cccgtcgtcc aaatagtcgg atagataaag tacgatatat gttcaataaa ataacttag
//
LOCUS       DASNNU010000305         5863 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.guided.123, whole genome shotgun sequence.
ACCESSION   DASNNU010000305 DASNNU010000000
VERSION     DASNNU010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.guided.123"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            382..846
                     /locus_tag="U8116_002934"
     CDS             382..846
                     /locus_tag="U8116_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN8435836.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            830..1459
                     /locus_tag="U8116_002935"
     CDS             830..1459
                     /locus_tag="U8116_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN8435837.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1813..3291
                     /gene="msr(C)"
                     /locus_tag="U8116_002936"
     CDS             1813..3291
                     /gene="msr(C)"
                     /locus_tag="U8116_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN8435838.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3338..3784
                     /locus_tag="U8116_002937"
     CDS             3338..3784
                     /locus_tag="U8116_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN8435839.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3983..4498)
                     /locus_tag="U8116_002938"
     CDS             complement(3983..4498)
                     /locus_tag="U8116_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN8435840.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4615..5817
                     /locus_tag="U8116_002939"
     CDS             4615..5817
                     /locus_tag="U8116_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN8435841.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1815 a          977 c         1237 g         1834 t
ORIGIN      
        1 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
       61 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
      121 tcggaaattt ttggcggtgt aaaattatct actgcagaag agctatcttc tatagtttga
      181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
      241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
      301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
      361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
      421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
      481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
      541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
      601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
      661 gaaaaagccc agaagaaaat caagcattgt gaaatggacc ataaagatac aggttccgtg
      721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
      781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
      841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
      901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
      961 tgtcaatatg aaaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
     1021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
     1081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tacaaccaag cttacgaatc
     1141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
     1201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
     1261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
     1321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
     1381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
     1441 gatcactatg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
     1501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
     1561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
     1621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
     1681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
     1741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
     1801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
     1861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
     1921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
     1981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
     2041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
     2101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
     2161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
     2221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
     2281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
     2341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
     2401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
     2461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
     2521 caaatcagac tggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
     2581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
     2641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
     2701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
     2761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
     2821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
     2881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
     2941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
     3001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
     3061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
     3121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
     3181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
     3241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
     3301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
     3361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
     3421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
     3481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
     3541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
     3601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
     3661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
     3721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
     3781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
     3841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
     3901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
     3961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
     4021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
     4081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
     4141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
     4201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
     4261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
     4321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
     4381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
     4441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
     4501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
     4561 tgtgcatatt gacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
     4621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
     4681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
     4741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
     4801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
     4861 cggatcggtt tgaaaaaaac attgatgaca ggattgttta tcatcgttgt attcgctggt
     4921 cttggcggat tatctgggaa tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc
     4981 gggaatgctt tatttgtctc tactgcttta gcaacgatcg tcagtatttt agttggtaac
     5041 acagaaaaag caatcatgat gtatgaagca gcacttggct gcggaatggc tgttggccct
     5101 ctagtcggtg gggtcttggg cagtggttca tggcgttatc cattttttgg ggtagcattt
     5161 ttgatgtttt gcggttttat agccctagcg gtacttttac ctgaaactga gaaaccaaag
     5221 agaaaagtag gcctttggga aggtgtcaaa gcgttaagca atcataaact tcgttcagtt
     5281 ggactaatcg cattgttata taatttcgga ttctttactt tacttgcgta tgcgccattt
     5341 ttattgattg gctattctga attagaagtc ggttttgtat tttttggctg gggtgtgctt
     5401 ctggctattg cttcgatttt cgttgcaccg gctcttgagc ggaagtttac gacttatcgg
     5461 acaattttag cagcatttgt cttattcatt atttgtttgc tgttattagg agctggaact
     5521 tctatgccag tccttgtacc gatcagcatt gtgttagcag gatttttcca aggaattatc
     5581 aatacactgt tgacgacgat tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg
     5641 tccagctata gttttgtccg ctttttcggt ggagctttag caccatttat tgcaggtgtg
     5701 attggtgaac atattggtga aagctatgcg ttttattttg caggtgccat tgttcttatc
     5761 agtgtatgtt gtatcattta tcaccgtaat tattttgcta caaatgaaga agaataagaa
     5821 atcagaggtt gtgaaaaagc tgtcctgcat caagtcataa gaa
//
LOCUS       DASNNU010000306         4339 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.guided.144, whole genome shotgun sequence.
ACCESSION   DASNNU010000306 DASNNU010000000
VERSION     DASNNU010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.guided.144"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            246..1214
                     /locus_tag="U8116_002940"
     CDS             246..1214
                     /locus_tag="U8116_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN8435842.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1207..2238
                     /gene="vanA"
                     /locus_tag="U8116_002941"
     CDS             1207..2238
                     /gene="vanA"
                     /locus_tag="U8116_002941"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN8435843.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2244..2852
                     /locus_tag="U8116_002942"
     CDS             2244..2852
                     /locus_tag="U8116_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEN8435844.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3280..4191
                     /locus_tag="U8116_002943"
     CDS             3280..4191
                     /locus_tag="U8116_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEN8435845.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1368 a          773 c         1006 g         1192 t
ORIGIN      
        1 gatgctggtg tcatccacca acatcaaaaa gtatagagcc ataatttttt aggaaaatct
       61 caaggttatc tttacttttt cttaggaaat taacaattta atattaagaa acggctcgtt
      121 cttacacggt agacttaata ccgtaagaac gagccgtttt cgttcttcag agaaagattt
      181 gacaagatta ccattggcat ccccgtttta tttggtgcct ttcacagaaa gggttggtct
      241 taattatgaa taacatcggc attactgttt atggatgtga gcaggatgag gcagatgcat
      301 tccatgctct ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc aacgtgtcgg
      361 aatccaacgc caaatccgcg cctttcaatc aatgtatcag tgtgggacat aaatcagaga
      421 tttccgcctc tattcttctt gcgctgaaga gagccggtgt gaaatatatt tctacccgaa
      481 gcatcggctg caatcatata gatacaactg ctgctaagag aatgggcatc actgtcgaca
      541 atgtggcgta ctcgccggat agcgttgccg attatactat gatgctaatt cttatggcag
      601 tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg ttggacagcg
      661 accgtggcaa ggtactcagc gacatgacag ttggtgtggt gggaacgggc cagataggca
      721 aagcggttat tgagcggctg cgaggatttg gatgtaaagt gttggcttat agtcgcagcc
      781 gaagtataga ggtaaactat gtaccgtttg atgagttgct gcaaaatagc gatatcgtta
      841 cgcttcatgt gccgctcaat acggatacgc actatattat cagccacgaa caaatacaga
      901 gaatgaagca aggagcattt cttatcaata ctgggcgcgg tccacttgta gatacctatg
      961 agttggttaa agcattagaa aacgggaaac tgggcggtgc cgcattggat gtattggaag
     1021 gagaggaaga gtttttctac tctgattgca cccaaaaacc aattgataat caatttttac
     1081 ttaaacttca aagaatgcct aacgtgataa tcacaccgca tacggcctat tataccgagc
     1141 aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg tttggatttt gaaaggagac
     1201 aggagcatga atagaataaa agttgcaata ctgtttgggg gttgctcaga ggagcatgac
     1261 gtatcggtaa aatctgcaat agagatagcc gctaacatta ataaagaaaa atacgagccg
     1321 ttatacattg gaattacgaa atctggtgta tggaaaatgt gcgaaaaacc ttgcgcggaa
     1381 tgggaaaacg acaattgcta ttcagctgta ctctcgccgg ataaaaaaat gcacggatta
     1441 cttgttaaaa agaaccatga atatgaaatc aaccatgttg atgtagcatt ttcagctttg
     1501 catggcaagt caggtgaaga tggatccata caaggtctgt ttgaattgtc cggtatccct
     1561 tttgtaggct gcgatattca aagctcagca atttgtatgg acaaatcgtt gacatacatc
     1621 gttgcgaaaa atgctgggat agctactccc gccttttggg ttattaataa agatgatagg
     1681 ccggtggcag ctacgtttac ctatcctgtt tttgttaagc cggcgcgttc aggctcatcc
     1741 ttcggtgtga aaaaagtcaa tagcgcggac gaattggact acgcaattga atcggcaaga
     1801 caatatgaca gcaaaatctt aattgagcag gctgtttcgg gctgtgaggt cggttgtgcg
     1861 gtattgggaa acagtgccgc gttagctgtt ggcgaggtgg accaaatcag gctgcagtac
     1921 ggaatctttc gtattcatca ggaagtcgag ccggaaaaag gctctgaaaa cgcagttata
     1981 accgttcccg cagacctttc agcagaggag cgaggacgga tacaggaaac ggcaaaaaaa
     2041 atatataaag cgctcggctg tagaggtcta gcccgtgtgg atatgttttt acaagataac
     2101 ggccgcattg tactgaacga agtcaatact ctgcccggtt tcacgtcata cagtcgttat
     2161 ccccgtatga tggccgctgc aggtattgca cttcccgaac tgattgaccg cttgatcgta
     2221 ttagcgttaa aggggtgata agcatggaaa taggatttac ttttttagat gaaatagtac
     2281 acggtgttcg ttgggacgct aaatatgcca cttgggataa tttcaccgga aaaccggttg
     2341 acggttatga agtaaatcgc attgtaggga catacgagtt ggctgaatcg cttttgaagg
     2401 caaaagaact ggctgctacc caagggtacg gattgcttct atgggacggt taccgtccta
     2461 atcgtgctgt aaactgtttt atgcaatggg ctgcacagcc ggaaaataac ctgacaaagg
     2521 aaagttatta tcccaatatt gaccgaactg agatgatttc aaaaggatac gtggcttcaa
     2581 aatcaagcca tagccgcggc agtgccattg atcttacgct ttatcgatta gacacgggtg
     2641 agcttgtacc aatggggagc cgatttgatt ttatggatga acgctctcat catgcggcaa
     2701 atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt gcgctccatc atggaaaaca
     2761 gtgggtttga agcatatagc ctcgaatggt ggcactatgt attaagagac gaaccatacc
     2821 ccaatagcta ttttgatttc cccgttaaat aaacttttaa ccgttgcacg gacaaactat
     2881 ataagctaac tctttcggca ggaaacccga cgtatgtaac tggttcttag ggaatttata
     2941 tatagtagat agtattgaag atgtaaggca gagcgatatt gcggtcatta tctgcgtgcg
     3001 ctgcggcaag atagcctgat aataagactg atcgcataga ggggtggtat ttcacaccgc
     3061 ccattgtcaa caggcagttc agcctcgtta aattcagcat gggtatcact tatgaaaatt
     3121 catctacatt ggtgataata gtaaatccag tagggcgaaa taattgactg taatttacgg
     3181 ggcaaaacgg cacaatctca aacgagattg tgccgtttaa ggggaagatt ctagaaatat
     3241 ttcatacttc caactatata gttaaggagg agactgaaaa tgaagaagtt gtttttttta
     3301 ttgttattgt tattcttaat atacttaggt tatgactacg ttaatgaagc actgttttct
     3361 caggaaaaag tcgaatttca aaattatgat caaaatccca aagaacattt agaaaatagt
     3421 gggacttctg aaaataccca agagaaaaca attacagaag aacaggttta tcaaggaaat
     3481 ctgctattaa tcaatagtaa atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg
     3541 aatttatcta aacatgacga attaataaat ggatacgggt tgcttgatag taatatttat
     3601 atgtcaaaag aaatagcaca aaaattttca gagatggtca atgatgctgt aaagggtggc
     3661 gttagtcatt ttattattaa tagtggctat cgagactttg atgagcaaag tgtgctttac
     3721 caagaaatgg gggctgagta tgccttacca gcaggttata gtgagcataa ttcaggttta
     3781 tcactagatg taggatcaag cttgacgaaa atggaacgag cccctgaagg aaagtggata
     3841 gaagaaaatg cttggaaata cgggttcatt ttacgttatc cagaggacaa aacagagtta
     3901 acaggaattc aatatgaacc atggcatatt cgctatgttg gtttaccaca tagtgcgatt
     3961 atgaaagaaa agaatttcgt tctcgaggaa tatatggatt acctaaaaga agaaaaaacc
     4021 atttctgtta gtgtaaatgg ggaaaaatat gagatctttt attatcctgt tactaaaaat
     4081 accaccattc atgtgccgac taatcttcgt tatgagatat caggaaacaa tatagacggt
     4141 gtaattgtga cagtgtttcc cggatcaaca catactaatt caaggaggta aggatggcgg
     4201 aatgaaacca acgaaattaa tgaacagcat tattgtacta gcacttttgg ggtaacgtta
     4261 gctttttaat ttaaaaccca cgttaactag gacattgcta tactaatgat acaacttaaa
     4321 caaaagaatt agaggaaat
//
LOCUS       DASNNU010000307         4261 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.guided.148, whole genome shotgun sequence.
ACCESSION   DASNNU010000307 DASNNU010000000
VERSION     DASNNU010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.guided.148"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            104..796
                     /locus_tag="U8116_002944"
     CDS             104..796
                     /locus_tag="U8116_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN8435846.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1123..1620
                     /gene="dfrG"
                     /locus_tag="U8116_002945"
     CDS             1123..1620
                     /gene="dfrG"
                     /locus_tag="U8116_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN8435847.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1692..3644)
                     /locus_tag="U8116_002946"
     CDS             complement(1692..3644)
                     /locus_tag="U8116_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN8435848.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3651..3887)
                     /locus_tag="U8116_002947"
     CDS             complement(3651..3887)
                     /locus_tag="U8116_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435849.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1436 a          719 c          707 g         1399 t
ORIGIN      
        1 gtatttatat taaaattaca tatgtaattt aaaaacgctt gacattgtgt ttgagatcac
       61 ttacttttag catgtaaaag tattggatta aggagctatg caaatgaaaa agaaatttat
      121 tactggtggg attgtcgttg catttctatg tctaattggc tacacaggca cgcggtacgc
      181 aaatgaaatt attttgtggg gtggagagac agatattgat acaatcaatg agaatctgac
      241 acagcttgat catacattga cagataaaga gcaagcgatt acagatctta ccgctcgact
      301 agcacaaaaa gaacagacat tgcaagattc aaaggcagat gtccaagaat accaaacaaa
      361 agtaaatgaa ttagaaaacc aaaaaaatca atcggcagca gaaaaacaaa acttagaaag
      421 tcaattagtc agcaaggatg cacaactaca aagcaaacaa aatgaaatca atgtcaaact
      481 agatgagatc aaccaaaaaa atgatgaaat caatcatctg aatgcagatt ggacagcaag
      541 actacaagaa gcacaagcaa aactttcaga attacaaaat actgtaaatg gtttgaatca
      601 acaaatcgca acactgacac aagaaaaaac aaatgtagaa actcagttaa cagatacaca
      661 aaagaaatta gacgaagcaa ctgctgaaaa ctcaagcttg aaacaatata ttcaaaagtt
      721 agaaaaagca aaagatgaag tggaagatac agctgataaa tctcagcaaa ttgttgaaga
      781 acatttgcgt aaataatctt tcttaacaag taaacctaaa ttagtaaaga agcaacagta
      841 atctatacta agtagttcag ttttggagta caaaatgaca aaatgtactt caaaatggac
      901 tacttttttc attttaactt acataccaaa ttacatacaa aatgatttat aaaatgcaat
      961 tgaaaatgat aattgaatga aaatttgtga ttttgtggta ttatgataat ataacttata
     1021 gaaaaaattg cttattcaac taacgggcag gataatgaga aaaggttttt tgatagaaat
     1081 aagaaattag atggtaaatt agttaaaaag gctgtgttat aaatgaaagt ttctttgatt
     1141 gctgcgatgg ataagaatag agtgattggc aaagagaatg acattccttg gaggattccc
     1201 aaggactggg aatatgttaa aaatactaca aagggacatc cgataatatt aggtaggaag
     1261 aaccttgaat caatcggaag agccttacct gacagaagaa atattattct gacgagagat
     1321 aaggggttta cctttaatgg ttgtgaaatt gttcattcaa tagaagatgt ttttgagtta
     1381 tgtaaaaacg aagaagaaat ttttattttc ggaggagaac agatttataa tttgtttttc
     1441 ccttatgttg agaaaatgta catcacaaaa atacatcatg aattcgaagg agatactttt
     1501 tttccagaag tgaattatga ggaatggaat gaggtatttg cccaaaaagg gataaagaat
     1561 gataaaaatc cgtataacta ctattttcat gtatatgaaa gaaaaaactt attgagttaa
     1621 cgggtgcgtt aattgaagaa gaacagtaaa gataaagcac aagagcttgg gattactcca
     1681 agctcttttt gtcatttgaa ataaattata tctttccaat caaattgttt atctgtaatc
     1741 cctaatcttt gagcaggtgt caaaaccttt ttatccgctg atttaaaagg gcgacaaaag
     1801 ttgtagtaag ttcttagaat tgtaatcgca aattgagcat acttcggatt gaagttagcg
     1861 taaatatagc tcttcttatc accacgagct gttgttaatg gtctttcaag agatgatata
     1921 taacgtctta tctgttgaat aaagctattg gtcgaatggt cattaacatt taatagcatc
     1981 tttgcgattt cgtttggttc cagagcagat aaatccgttc tacaatcaac tgaataaaat
     2041 cctttatcct tagtggctaa tggatgtttg atagggttat cggcatattt gcgatatctt
     2101 tctccatctt tactaatagc ctcttcgtgg aattgatgac ttgttagtaa ttcagttaga
     2161 taatgagatg ccaaagtccg tagaaacttt gttttgaatc ctctgatatc tccccaatcg
     2221 agcagttctg cttttgcttg tccgaattct tttaagcatt gttttctgct tttcgtcttg
     2281 ttaacttggc aaataaaatg atgtgcatcg ctcaacctta attctttcgt gaatactcta
     2341 taaaaggcgt ttctaataga gaagtcatca tcactaatca ttcgccattc gtcggcatta
     2401 accatttgtt taattaacca atagtgagca gttgtagtat aaggagcatc aacatttaaa
     2461 ccatcgacaa atctactcct attagtaatt ttccctaatt cgtgaagata agcattccta
     2521 ttttcgctat cgttagcgga tggttcttgt ggataataag accaatctaa cctatcattt
     2581 ttacgactaa aagtattgag atggtcttct ttgaattttc ttgtatcttc atttaattca
     2641 tccattgaaa tattccaatc ataggcaaca tcagaacgga acacatatct tgataagacc
     2701 tcagcagata caactatata cgtttgcata ttaaggtctt caaaccccgt atatttcttt
     2761 gaaccttgtc ctttcttacg gacgttattt aggtagtagt gcatcttgtc cgtattaagc
     2821 cacatctcgt taaatttctt tgtttgaagg ggttgtgtct catatcgttc taaaaactcc
     2881 aaacaacgcc tatacagcca ctccagctta tgatagtagg ttccaactcc aattcctaaa
     2941 atatcacaag tacgactgac tgaaaccctt ccaacaacca tcttagcgaa cattggaaga
     3001 atggtgttct tttgttggtg ataagtagtg gtttcttctc tttttggtag aacattagtg
     3061 aacttcttac acgccttaca ttgatagcgt tgagatttgc ccctactttt cccccttttg
     3121 taaaattgct tcggctcatt gaatggggtt gattcttctt ccgagcagcc ttctttatga
     3181 aattgataat ctggctcaac atcttggatg ctattgattt ctataaggcg tttaatttct
     3241 tcaacaacag accaattaga taaaggggtt actgtacagt tttgcgatac acctctgcca
     3301 ataggattgt cattgcagta cataccttta tgacctttat ctttggaact accagtcatt
     3361 ttatatcgac ttggtttccc ttttacatct ttgaatttgt gctgttcttt cccaaaatta
     3421 caacaaaacg gattgatgca gtaattatat tgaatgggat attcagaacc gttccattta
     3481 aaagacgttg gcaagaacaa cattttgtta taccttgttg caaatcgttt tggagttaat
     3541 aaatcatatt gtttagccct atcttttaat tcttcgtttg aaatcggaat tttaacatct
     3601 acaatttcat cttccttcgt ggctaatctt ttaagcttgg tcattgtcta tcaacccact
     3661 ctatctttga tacccatttt ttcaacaatc cgcttattac ttcgcttgtt ggcaatccat
     3721 tcaataaact tcttatcgtc taaaatgttc aataagaatt catccactgc ttcgctttca
     3781 gtacgctctg catattcagc gtattgctta actatcaccc ttacttgctc tgatatttcc
     3841 caatccacag acttagcgtt cttatttttt ggctcaagaa attgcatagg aaaatgcacc
     3901 cctttttgct tagtaaattg taaccatttt aacacaccaa tttgcattcc tacaataatt
     3961 atttcacgtt cgcattttta tatacatttt aacttacatt ttgtggtgta aattaacaac
     4021 aaacgttgat ttaacaataa aaaacgagta tcaaattcag ttgcaatttg atactcattt
     4081 aggtttacat tttcactttt tgtactccaa aactgaacta cttaaatcta tactgctgcc
     4141 tctttttttt agtacatgtc cttcatcttc gcaggaagtg ccaatatgat tttaagaaag
     4201 gtaatagaca aaaagtcaaa aagataaaac agagtttctt ctttacaaag ggaatgtatg
     4261 t
//
LOCUS       DASNNU010000308         3291 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.guided.161, whole genome shotgun sequence.
ACCESSION   DASNNU010000308 DASNNU010000000
VERSION     DASNNU010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.guided.161"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            24..752
                     /locus_tag="U8116_002948"
     CDS             24..752
                     /locus_tag="U8116_002948"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN8435850.1"
                     /translation="MLRGGSGCSDGGLGLLQSLGASLEGYTEGNPLLSTKKLHFDRRN
                     VLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFWE
                     VDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVIITGEGRIDQ
                     QTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAME
                     HRQTLEKTKILGQTLSRFFTILPK"
     gene            1010..2512
                     /gene="eat(A)"
                     /locus_tag="U8116_002949"
     CDS             1010..2512
                     /gene="eat(A)"
                     /locus_tag="U8116_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN8435851.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2540..3106
                     /locus_tag="U8116_002950"
     CDS             2540..3106
                     /locus_tag="U8116_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN8435852.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         1127 a          557 c          722 g          885 t
ORIGIN      
        1 ataaaaacta ttagacaaat gatattttac gaggaggaag cgggtgtagc gatggtggtc
       61 taggattact ccaatccttg ggtgcttctt tagaagggta cacagaagga aatcctttat
      121 tatctactaa aaaacttcat tttgatcgaa gaaacgttct tgtttctaaa gtaaaattga
      181 ttatcgctgc agatgttacc aatccatata caggtcaaca aggagctgct tggcaatttg
      241 gaagacaaaa aggaggaata gatacgacgc tagctttctt agataaacaa gcaggaaaga
      301 ttgctcagca aataaaagat ttctgggagg tggatttaga tgatattcca ggaagtggag
      361 cagcaggcgg tattggtgga gccctttttt tgttgggagc gaagatggtt tctggtttcg
      421 atttagtcag ccaaatcact ggattggaaa aaaagatcga acaagcagat gtcatcatta
      481 caggagaagg gcggatcgat cagcaaacga tccacggaaa agttccatac ggtgtagcag
      541 tgcttgcaaa aaaatatcga aaaccaataa tcgctatttg cggaagtaga gaccaagagc
      601 tagaaggact ttctgatttt ctcccaattg tcttaagtat tcatttgggt ccaataggtt
      661 tagaagaagc aatggaacac aggcaaacat tagaaaaaac aaaaatctta ggacagacac
      721 ttagcagatt tttcaccatc ctcccgaagt agttgacaaa atcattttct ttatgctatc
      781 gtaatttcaa atataggaca aagtgaggga tccgtgcatt gaaaaattaa gtcgattgat
      841 gaaagctgca ttcagactgt accttgtgga gacaaggaag cagtgtgcag attttcggga
      901 ttggcttggt tttttcgatg tatgggtctt tttgtgtgta tccaaccctt gcatcctctt
      961 tagccagttc tgaaaatagg actggtcttt ttttacgagg tgatggaaaa tgtctaaaat
     1021 cgaaataaaa aatctgacat tcggctacga cagccaaggc acattattat ttgaacaagc
     1081 aaatctaaat tttgacacac aatggaaact aggacttatc ggacgaaacg gtcgaggaaa
     1141 gacaacttta ctgaatattc tacaaaacaa actaccttat caagggcaag taatccatca
     1201 gcaagaattt gcctatttcc cgcaacagac aaaagataaa gaacgtttaa cctattacgt
     1261 gttaaatgat attacggatt ttgagatatg ggaaatcgaa agagagctcc aattgatgca
     1321 aacagatcct gaaatcttat ggagagaatt cagcacacta tcggggggag agaagacaaa
     1381 agtcctactg gcacttttat ttgtggatga cactcatttc ccgttaatcg atgaaccaac
     1441 gaatcatttg gatatctctg gtagaaaaca agtagcggct tatttgaaaa agaaaaaaca
     1501 aggcttcatc gtggtcagcc atgaccgggg atttatcgat gaagtagtgg accatgtttt
     1561 agcaatcgaa aaaagtcaac tggaacttta tcaagggaat ttctctatct atgaagaaca
     1621 gaaaaaactt cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa aagaagtcag
     1681 taggctaaag aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag aaggagataa
     1741 aacaaagaaa caagtcggat tcatcgatac tgaatctaga cgagtgaata aaggagcagt
     1801 gggtgctgat gctgcacgga cgatgaaacg atccaaagca atcgtgaatc ggatggagac
     1861 ccagatcagc gagaaagaaa aactattaaa agatatcgaa tatatcgatt ctttgacgat
     1921 gaatagccaa gcgtctcacc ataagcgact tttaagcgta gaagatcttc aattagggta
     1981 tgaaaatctg ttattcgagc caattcattt tacaatcgag cctcatcagc gggtggcgat
     2041 ttcaggtcct aacggtgcag gaaagtcatc cattatccat tatcttctgg gggcattcaa
     2101 cggcaaggtt ataggagaaa aataccagcc aaaacatctg agcattagtt atgcaagcca
     2161 aaattatgaa gacaatcgag gaacgttggc ggaatttgca gagaaaaacc aagtagacta
     2221 ccaagcattt ttgaacaacc tccgaaagct tgggatggaa agagatgttt ttcataacaa
     2281 gatcgagcag atgagtatgg gccaacggaa aaaagtggaa ttggctaaat ctttatcaca
     2341 gccagctgaa ctatatacat gggatgaacc attgaattat ttggatgtct tcaatcaaga
     2401 acaattagaa caactgatct tgaacgtgaa acctgccatg ttactagtgg aacatgatca
     2461 aaccttcctg gataaagtat ctactgagat tatttctctt gagagaatct aacccttgta
     2521 atctcaagaa caacaaggta tggttaaggg gaactttgcc aacgaaggag agaatcacat
     2581 gctttatgta acgagacacg gagaaacaac ttggaacgca caaggattag tttgcggacg
     2641 ggcagatgtg ccgctgactg aaaaaggaca gatgcaggca cagaaactag cagaaaaggt
     2701 agtagatttg cctgttccaa tcacgaaaat catccattcc cctcttcaac gagcaagaga
     2761 tacagcacaa gcagttgctg atcgtctcag cttgccgctg accgttgatg aacgattggt
     2821 agaaatggat tttggggatt atgatggact gccaagcaaa gatgagaact ttcaaaaagc
     2881 acgtttagcg ttcgctgtac gattcccaaa tggggagtca gtcttagatg tgtatgcacg
     2941 gatcgttcct ttattgaaag aatgcatcga agacgaagaa aatgtctatc tgttagtttg
     3001 ccataatgcc ttgataagag tgatcaatgc ttattttcat ccgatgccta atgaggggtt
     3061 ctttacattc atggtagata atacagaatt ggtttcttat gagtaattat gtttttgtgt
     3121 tatttaaata aaatatttca aggcactgct tttgcttagc agtgcctttt ttaggttatg
     3181 acaattcttt tgataatata aactcaacct ttagaaatga aatttaatta ttttaataaa
     3241 agtattgaaa ttttgtttct ttagatatat actctagtca tagaaatgaa g
//
LOCUS       DASNNU010000309         2556 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.guided.174, whole genome shotgun sequence.
ACCESSION   DASNNU010000309 DASNNU010000000
VERSION     DASNNU010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.guided.174"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..458
                     /locus_tag="U8116_002951"
     CDS             <1..458
                     /locus_tag="U8116_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN8435853.1"
                     /translation="LFDSRFKTLQQNLQEDDIIYVTDLTRITRSTQDLFELIDNIRDK
                     KASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKG
                     RLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            672..1367
                     /gene="vanR"
                     /locus_tag="U8116_002952"
     CDS             672..1367
                     /gene="vanR"
                     /locus_tag="U8116_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN8435854.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1345..2499
                     /locus_tag="U8116_002953"
     CDS             1345..2499
                     /locus_tag="U8116_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN8435855.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVQITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          882 a          454 c          536 g          684 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacct acaggaagat gacatcattt
       61 atgttacaga cttaactcga atcactcgta gtacacaaga tctatttgaa ttaatcgata
      121 acatacgaga taaaaaggca agtttaaaat cactaaaaga tacatggctt gatttatcag
      181 aagataatcc atacagccaa ttcttaatta ctgtaatggc tggtgttaac caattagagc
      241 gagatcttat tcggatgaga caacgtgaag ggattgaatt ggctaagaaa gaaggaaagt
      301 ttaaaggtcg attaaagaag tatcataaaa atcacgcagg aatgaattat gcggtaaagc
      361 tatataaaga aggaaatatg actgtaaatc aaatttgtga aattactaat gtatctaggg
      421 cttcattata caggaaatta tcagaagtga ataattagcc attctgtatt ccgctaatgg
      481 gcaatatttt taaagaagaa aaggaaacta taaaatatta acagcctcct agcgatgccg
      541 aaaagccctt tgataaaaaa agaatcatca tcttaagaaa ttcttagtca tttattatgt
      601 aaatgcttat aaattcggcc ctataatctg ataaattatt aagggcaaac ttatgtgaaa
      661 gggtgataac tatgagcgat aaaatactta ttgtggatga tgaacatgaa attgccgatt
      721 tggttgaatt atacttaaaa aacgagaatt atacggtttt caaatactat accgccaaag
      781 aagcattgga atgtatagac aagtctgaga ttgaccttgc catattggac atcatgcttc
      841 ccggcacaag cggccttact atctgtcaaa aaataaggga caagcacacc tatccgatta
      901 tcatgctgac cgggaaagat acagaggtag ataaaattac agggttaaca atcggcgcgg
      961 atgattatat aacgaagccc tttcgcccac tggagttaat tgctcgggta aaggcccagt
     1021 tgcgccgata caaaaaattc agtggagtaa aggagcagaa cgaaaatgtt atcgtccact
     1081 ccggccttgt cattaatgtt aacacccatg agtgttatct gaacgagaag cagttatccc
     1141 ttactcccac cgagttttca atactgcgaa tcctctgtga aaacaagggg aatgtggtta
     1201 gctccgagct gctatttcat gagatatggg gcgacgaata tttcagcaag agcaacaaca
     1261 ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa cgacaccatt gataatccga
     1321 aatatataaa aacggtatgg ggggttggtt ataaaattga aaaataaaaa aaacgactat
     1381 tccaaactag aacgaaaact ttacatgtat atcgttgcaa ttgttgtggt agcaattgta
     1441 ttcgtgttgt atattcgttc aatgatccga gggaaacttg gggattggat cttaagtatt
     1501 ttggaaaaca aatatgactt aaatcacctg gacgcgatga aattatatca atattccata
     1561 cggaacaata tagatatctt tatttatgtg gcgattgtca ttagtattct tattctatgt
     1621 cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga taaataccgg cattgatgta
     1681 cttattcaga acgaagataa acaaattgag ctttctgcgg aaatggatgt tatggaacaa
     1741 aagctcaaca cattaaaacg gactctggaa aagcgagagc aggatgcaaa gctggccgaa
     1801 caaagaaaaa atgacgttgt tatgtacttg gcgcacgata ttaaaacgcc ccttacatcc
     1861 attatcggtt atttgagcct gcttgacgag gctccagaca tgccggtaga tcaaaaggca
     1921 aagtatgtgc aaatcacgtt ggacaaagcg tatcgactcg aacagctaat cgacgagttt
     1981 tttgagatta cacggtataa cctacaaacg ataacgctaa caaaaacgca catagaccta
     2041 tactatatgc tggtgcagat gaccgatgaa ttttatcctc agctttccgc acatggaaaa
     2101 caggcggtta ttcacgcccc cgaggatctg accgtgtccg gcgaccctga taaactcgcg
     2161 agagtcttta acaacatttt gaaaaacgcc gctgcataca gtgaggataa cagcatcatt
     2221 gacattaccg cgggcctctc cggggatgtg gtgtcaatcg aattcaagaa cactggaagc
     2281 atcccaaaag ataagctagc tgccatattt gaaaagttct ataggctgga caatgctcgt
     2341 tcttccgata cgggtggcgc gggacttgga ttggcgattg caaaagaaat tattgttcag
     2401 catggagggc agatttacgc ggaaagcaat gataactata cgacgtttag ggtagagctt
     2461 ccagcgatgc cagacttggt tgataaaagg aggtcctaag agatgtatat aattttggct
     2521 ctttgtcaaa tgatgttggt aaagagaaat cttaga
//
LOCUS       DASNNU010000310         1346 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.guided.222, whole genome shotgun sequence.
ACCESSION   DASNNU010000310 DASNNU010000000
VERSION     DASNNU010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.guided.222"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            630..1178
                     /gene="aac(6')"
                     /locus_tag="U8116_002954"
     CDS             630..1178
                     /gene="aac(6')"
                     /locus_tag="U8116_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN8435856.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          467 a          201 c          265 g          413 t
ORIGIN      
        1 tctttgacga ttccatttgc tttttcatac gcatctgttc caatggtata atttggtaag
       61 aaaacacttt gactcatatt catttccttc tttcttcgtt gaaacgggtt aaaattttaa
      121 agagtgatcg atctgactgc ggctgtagat ggagtagagg tgaacctaag acaggataag
      181 tgatgaataa ggtggcatga acaccttgaa atcatcaata agtatcctaa aaacgaacaa
      241 gtacaaaaac attgaaaacg acaataatga atttttgttc ctcttagtat ttctaccgat
      301 tccctttgct tttttgtaat tatctaaaaa ttctaaaaat taagattatt atatgaaaac
      361 aggttttatt tgtcaattca taattggttt tttttaaagc aaaagataat ctatatcatg
      421 atgaacaaac agagtttttt cctccaattg atggctttcc tgatctttcc tattttgaat
      481 aaagtgtttc tgtataattt ttttatttta taagtaagat aaataatgaa agaatagaga
      541 tactgattgg tagctaaaga tttttgagcg agaacatttc gttttttcaa aaggactgtt
      601 agaataagca agaacaaggg ggaagacgta tgataatcag tgaatttgac cgtaataatc
      661 cagtattgaa agatcagctt tctgatttac tgagactgac ttggccggaa gaatatggag
      721 acagctcggc agaagaagta gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg
      781 tagaccaaga tgagttagta ggatttattg gtgcaatccc tcaatacggt atcacaggtt
      841 gggaattgca tccattagtt gtagaaagct cccgacgaaa gaaccaaata ggtactcgat
      901 tagtcaatta cttagaaaaa gaagtagctt ccagaggagg aatcacgatt tatttaggta
      961 cggatgattt agaccatgga acaacgttaa gtcaaacgga cctgtatgag catacatttg
     1021 ataaagtggc ttccatccag aaccttcgtg aacatccgta tgaattctat gaaaaattag
     1081 gttataaaat cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg gatatttgga
     1141 tggcaaaaac gattattcct cgaccaaatt ctcaataata aaaagaggtt gtgacagaag
     1201 taatcagctt caagaaataa gaaggaattt acgaaaatta cttcttgaat ttttgtgaaa
     1261 ttccaactta tttccgaaga agctactttt ggaacaccgt gtaccagagg ttgtgaaaaa
     1321 gtcgcttagt ttcgagtagt aagaaa
//
LOCUS       DASNNU010000311          943 bp    DNA     linear   BCT 19-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate V4734
            SAMN38505789-rid20627643.guided.244, whole genome shotgun sequence.
ACCESSION   DASNNU010000311 DASNNU010000000
VERSION     DASNNU010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38505789
            Sequence Read Archive: SRR26992693
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002035364.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002035364.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/20/2023 00:00:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 24 of 59
            Pseudo Genes (internal stop)      :: 16 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38505789-rid20627643.guided.244"
                     /isolate="V4734"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2022-06-17"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(628..918)
                     /locus_tag="U8116_002955"
     CDS             complement(628..918)
                     /locus_tag="U8116_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN8435857.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          285 a          198 c          143 g          317 t
ORIGIN      
        1 acttaattgt taaatacctt atttgatgca aaagaaatct acgatagtca acaaaaaaat
       61 tttcattata tccagaaaaa tttttcttag ttaaggacct atggattgca gtaatgcaga
      121 attcgtcgaa taaactgcca aagacataca atcatattgc ttttaaaatt gacgaacaag
      181 atatagattc gtttgtctca aaaatccaaa tgttaggttt aactgtcgaa ccaggacgtt
      241 ctcgtgtgaa aggcgaggct tcttcaattt acttttacga ctatgataat cacctcttcg
      301 agttacatac cggtactctc caggaacgtt taaaatccta caattccact acctgagact
      361 agtaatgact agtctctttt tatggcctct gatcccccca ttgagcgaat actaataggg
      421 cttcgaattg atctgttcaa tctttaagca atatccctca aatgagtttg gaaagtttag
      481 tgattcatca caaatactta aatacatgta gttatatata gattgaggtt cttgtgagcg
      541 tatgctcgtg tgggttcaaa tcccatcgtc tgcatccatc tttccaaaac aagcggctag
      601 gacatttttg tctcagccgc ttgttttcta gtttattctt tcatcttgtc ggaccttttt
      661 tcttgggtat agacttttct catattctac cacaattttg ggtttcccta acacatgata
      721 ggatctcaca acaaattgct ttctattatt ataataacct gtaacgacta acttcatccc
      781 ttcgtctact tcagctagga agttcaaact atgggaagca atcaaacaat tgaccccatt
      841 taaactgaaa cggatcaacg gtctggcact aacttgcaaa gtacggatct tatctactgt
      901 tccatatttt ttctccataa ccttcacctc aataccatta tac
//
